RapGAP1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	RapGAP1
Entrez Gene ID	34032
Full Name	Rap GTPase activating protein 1
Synonyms	CG13789,CG13790,CG13791,CG33529,CG34374,CG44086,CG6682,Dmel\CG44086,Dmel_CG13790,Dmel_CG13791,Dmel_CG33529,Dmel_CG34374,FBpp0111515,Q9VM02_DROME,RapGap1,Rapgap1,rapgap1
General protein information	Preferred Names Rap GTPase activating protein 1 Names CG44086 gene product from transcript CG44086-RH CG44086-PD CG44086-PE CG44086-PF CG44086-PH CG44086-PI RapGAP1-PD RapGAP1-PE RapGAP1-PF RapGAP1-PH RapGAP1-PI Rapgap1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 28B1-28B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001103644.3	NP_001097114.2	Rap GTPase activating protein 1, isoform H
NM_144299.3	NP_652556.2	Rap GTPase activating protein 1, isoform D
NM_001014478.2	NP_001014478.1	Rap GTPase activating protein 1, isoform E
NM_001273279.1	NP_001260208.1	Rap GTPase activating protein 1, isoform I
NM_001014479.2	NP_001014479.1	Rap GTPase activating protein 1, isoform F

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Rapgap1	NP_001014479.1
Gallus gallus (chicken)	LOC395647	NP_990174.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009533	XP_310156.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RapGAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RapGAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14043	NM_001273279.1 Latest version!	Drosophila melanogaster Rap GTPase activating protein 1 (RapGAP1), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa14043	NM_144299.3 Latest version!	Drosophila melanogaster Rap GTPase activating protein 1 (RapGAP1), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa16368	NM_001103644.3 Latest version!	Drosophila melanogaster Rap GTPase activating protein 1 (RapGAP1), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa16369	NM_001014479.2 Latest version!	Drosophila melanogaster Rap GTPase activating protein 1 (RapGAP1), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa16370	NM_001014478.2 Latest version!	Drosophila melanogaster Rap GTPase activating protein 1 (RapGAP1), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RapGAP1 ( NM_144299.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_652556.2 Drosophila melanogaster Rap GTPase activating protein 1 (RapGAP1), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa14043
Clone ID Related Accession (Same CDS sequence)	NM_144299.3 , NM_001273279.1
Accession Version	NM_144299.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rap GTPase activating protein 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_144299.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGAAGA TACACATGCT GGAGCAGGTC GTCAACACGG AAAATGTCTG CGAGAATGCC 
CAGGAGCTAT CACCAAAGCG CAAATTGGCG GGTCAGGGGC AAGTGGGTGG TGGCAGCTCC 
TCCTCCAACG GAGGCACGCC CACCGTCTCG GCCACCTCGT CGCCCCACCA CCAGCCAACG 
CCCCCCCAGC GGCACATTCA CACGGGCGGT GGCAAGAGCG CCAGCCAAGG ATCATCACCA 
GCTGGACGTC AGCATGCCTT TATGGCCAAC ACACAGAAGC AGCAACCGGC GGGCGGAGGA 
AGTGGCTCTG GCAGCGTCAC CCACAGTCCG GAACGCCTGC GCGGAGCAAC CACAGCCACC 
CAGGATCTCT TCGAGCTGCT CGAGCGTGTG CAGTGCTCGC GACTCGACGA CCAACGCTGC 
GTCCTGCCCG CATACTTTTC TCAGAACCAC CATCGCGGCA GTAATGCGAG TGAGGAGCGC 
GTGCCCCAGC CACACTCACC CCACGACAGT CACGCCCATC CGCACAACGG TGGGCTGCAG 
CACTCGGGAT CGCGGGCAAC ACTGCGCCAC TCGATGAATC ACTCGAGCAG CTCCACAGTC 
TCGGGCTCCG CCTGCAGCTC GACGCCCGCC TCCCCGCAAC TGAGTGGGGT GGTCAGCGCC 
TCTGGATGCC AGCTGCTGTC CAACGGACAC CACTACCAAT CTCAGTCGTC GGTGAGCTCC 
AACTCATCGA TCGTTTCGGC CATTCCCGCC TCGCAGAGAT TGCTGGAGGA GGCGCTGGCC 
AAACCAGCAC CGTACCCCAT GATCTTGCTG CCACTGCACG GTGGTTACTG GATGGATGGC 
ACCGAACATG AGTGCGGCTA CGATGCGAGA GGAAATCCTC TGCTACCACA GACTACCTGG 
ATGGCAAAGT TCGAGACGGA CGATACGGCC AAGTGCTACA GGAGATTCTA TGCCGCCAGG 
GAGCACTCGA ATTTGATTGG ACTGGATGAG CAGTTGGGAC CGATTTTGCT TTCTATTAAA 
ACGGAAAATG TAGCCAACCA GGAGCACATG CGAATCCTGA TGAGATTGAG AACTGGTACG 
ATGCATGAGC TTCTACCGGT ATCATGCCTA TTACCACAGC CAAGTCCGGC AAAAATGGCG 
CATACACTGA ACGAAAATAT CACAGTCGAG CATTTCATGC CGATTTTGTG CCCCAAGGCC 
TCGCAACTCA TCAGTGTATA CGACGAGCAT GTTCTCGTTT CGCACTTCAA GTTTGGAGTG 
CTCTACCAAA GATATGGACA GACCACCGAG GAGGAACTTT TCGGTAACCA GCAGACATCA 
CCAGCTTTCG ACGAATTCTT GGATGTCTTG GGTCAAAGGA TACGTCTGAA GGATCACAAG 
GGCTACAGGG GTGGGCTTGA CATTCAGAAT GGTCACACGG GCGACACAGC TGTCTATGAG 
GTCTTCAAGG AGCGAGAGAT CATGTTCCAT GTATCCACAC TATTGCCGCA CACCGAAGGC 
GATCCACAGC AGCTCCAGAG GAAGCGACAC ATTGGCAATG ACATTGTGGC CATCGTTTTT 
CAGGAGACTA ACACTCCATT CTCACCCGAC ATGATCGCCA GTCACTTTCT GCACGCTTTC 
ATCGTGGTCC AGCCCATTGA GCCGAATACA CCACATACCC GGTACAAGGT CAGCGTAACC 
GCCAGGGATG ATGTTCCCTT CTTTGGTCCC ACTCTGCCGA ATCCCGCAGT TTTCCGCAAG 
GGACAGGAGT TCAAGGAGTT CATCTTGACC AAGCTGATCA ATGCTGAGAA TGCGTGCTAC 
AAAGCTGAGA AGTTTGCAAA GTTGGAACAG CGCACCAGGA CCTCTCTGCT CCAGAATTTA 
TGCGAGGAAC TGCGGGAGAA GACTCGCGAC TTTCTGGGAA CCGATCTTTC CCAGACGTCG 
GCAGGAAGTC CTACACCCGA GACCCCGAAA GCAGAAAGTG GTAGTGGCGG CAATGCCGGC 
TCCCGCTTTA TCGATACGGT GAAGAAGGCT TTAATCATGA GGGTGCGCTC ACAGAGCGTG 
GACACCGGAA ATGGTCATGG ACCTGGACAA ACGGATAAAT TCAGTGTGAA CACGAAGCTG 
GGCACATTGA ATTCCAAGAA GGAGACCCAT CCCGAAATGC AGACACCCAA TCTAAATACC 
TGCAGTCGCA CGGCCTCCAA GTCCTCATCC AAGTCGAAGT CATCGAACGA GTCCACTTCC 
TCCTCCCCGG ACATTACCTC TCGCCAGGCG AACAACAACA ATACCAATTC TATAGGTGGA 
CACCTCCCCC AAAATGGAAC CGGCGGGGGC GTGGTAGCAG CGGCTGTGAG TGCCGCCCAA 
AACGGAGTGG TCGCAGTGGC CACCATGTCG GAAACGAGCG ATGATTCCAG TCTGAATAGC 
GTGGATCTCG ATCCAATGAT GGCGCACTTG GACGGCGGAG CCACCTACAT TGACAGTGAT 
ACTGGGCTAG AAAGCATGAG TTCGGCAGAG GCCACCACCA AGGCGTGCTC CCTGTGCTTA 
GATGGTGTCC AGTCCACCAT AATGGGTAGT TCCCCCAGCA ACGAGACGAT CGTGAAGATC 
GAGAATCTGC GCCAGGAGGT CACACGTTTG AAGTGCGACA AGCTGGATCT ACTCAGACAG 
AATGTGACCT GTCAGCGCGA CATCAAGCGA CTTCGGGAGC GGGAACTTTC CCTTCAAGGC 
GATCTATCGG CAGCTGGCAG AGAAATCCTA CGACTGCGGG ATCTCCTCAA GGAATATATG 
CCCGATACAG CTTCAGCACC ACTCTCCATA TCGCCCATCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_652556.2
CDS	524..3325
Translation	MLKIHMLEQVVNTENVCENAQELSPKRKLAGQGQVGGGSSSSNGGTPTVSATSSPHHQPTPPQRHIHTGGGKSASQGSSPAGRQHAFMANTQKQQPAGGGSGSGSVTHSPERLRGATTATQDLFELLERVQCSRLDDQRCVLPAYFSQNHHRGSNASEERVPQPHSPHDSHAHPHNGGLQHSGSRATLRHSMNHSSSSTVSGSACSSTPASPQLSGVVSASGCQLLSNGHHYQSQSSVSSNSSIVSAIPASQRLLEEALAKPAPYPMILLPLHGGYWMDGTEHECGYDARGNPLLPQTTWMAKFETDDTAKCYRRFYAAREHSNLIGLDEQLGPILLSIKTENVANQEHMRILMRLRTGTMHELLPVSCLLPQPSPAKMAHTLNENITVEHFMPILCPKASQLISVYDEHVLVSHFKFGVLYQRYGQTTEEELFGNQQTSPAFDEFLDVLGQRIRLKDHKGYRGGLDIQNGHTGDTAVYEVFKEREIMFHVSTLLPHTEGDPQQLQRKRHIGNDIVAIVFQETNTPFSPDMIASHFLHAFIVVQPIEPNTPHTRYKVSVTARDDVPFFGPTLPNPAVFRKGQEFKEFILTKLINAENACYKAEKFAKLEQRTRTSLLQNLCEELREKTRDFLGTDLSQTSAGSPTPETPKAESGSGGNAGSRFIDTVKKALIMRVRSQSVDTGNGHGPGQTDKFSVNTKLGTLNSKKETHPEMQTPNLNTCSRTASKSSSKSKSSNESTSSSPDITSRQANNNNTNSIGGHLPQNGTGGGVVAAAVSAAQNGVVAVATMSETSDDSSLNSVDLDPMMAHLDGGATYIDSDTGLESMSSAEATTKACSLCLDGVQSTIMGSSPSNETIVKIENLRQEVTRLKCDKLDLLRQNVTCQRDIKRLRERELSLQGDLSAAGREILRLRDLLKEYMPDTASAPLSISPI

Target ORF information:

RefSeq Version	NM_144299.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rap GTPase activating protein 1 (RapGAP1), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144299.3

ORF Insert Sequence:

ATGCTGAAGA TACACATGCT GGAGCAGGTC GTCAACACGG AAAATGTCTG CGAGAATGCC 
CAGGAGCTAT CACCAAAGCG CAAATTGGCG GGTCAGGGGC AAGTGGGTGG TGGCAGCTCC 
TCCTCCAACG GAGGCACGCC CACCGTCTCG GCCACCTCGT CGCCCCACCA CCAGCCAACG 
CCCCCCCAGC GGCACATTCA CACGGGCGGT GGCAAGAGCG CCAGCCAAGG ATCATCACCA 
GCTGGACGTC AGCATGCCTT TATGGCCAAC ACACAGAAGC AGCAACCGGC GGGCGGAGGA 
AGTGGCTCTG GCAGCGTCAC CCACAGTCCG GAACGCCTGC GCGGAGCAAC CACAGCCACC 
CAGGATCTCT TCGAGCTGCT CGAGCGTGTG CAGTGCTCGC GACTCGACGA CCAACGCTGC 
GTCCTGCCCG CATACTTTTC TCAGAACCAC CATCGCGGCA GTAATGCGAG TGAGGAGCGC 
GTGCCCCAGC CACACTCACC CCACGACAGT CACGCCCATC CGCACAACGG TGGGCTGCAG 
CACTCGGGAT CGCGGGCAAC ACTGCGCCAC TCGATGAATC ACTCGAGCAG CTCCACAGTC 
TCGGGCTCCG CCTGCAGCTC GACGCCCGCC TCCCCGCAAC TGAGTGGGGT GGTCAGCGCC 
TCTGGATGCC AGCTGCTGTC CAACGGACAC CACTACCAAT CTCAGTCGTC GGTGAGCTCC 
AACTCATCGA TCGTTTCGGC CATTCCCGCC TCGCAGAGAT TGCTGGAGGA GGCGCTGGCC 
AAACCAGCAC CGTACCCCAT GATCTTGCTG CCACTGCACG GTGGTTACTG GATGGATGGC 
ACCGAACATG AGTGCGGCTA CGATGCGAGA GGAAATCCTC TGCTACCACA GACTACCTGG 
ATGGCAAAGT TCGAGACGGA CGATACGGCC AAGTGCTACA GGAGATTCTA TGCCGCCAGG 
GAGCACTCGA ATTTGATTGG ACTGGATGAG CAGTTGGGAC CGATTTTGCT TTCTATTAAA 
ACGGAAAATG TAGCCAACCA GGAGCACATG CGAATCCTGA TGAGATTGAG AACTGGTACG 
ATGCATGAGC TTCTACCGGT ATCATGCCTA TTACCACAGC CAAGTCCGGC AAAAATGGCG 
CATACACTGA ACGAAAATAT CACAGTCGAG CATTTCATGC CGATTTTGTG CCCCAAGGCC 
TCGCAACTCA TCAGTGTATA CGACGAGCAT GTTCTCGTTT CGCACTTCAA GTTTGGAGTG 
CTCTACCAAA GATATGGACA GACCACCGAG GAGGAACTTT TCGGTAACCA GCAGACATCA 
CCAGCTTTCG ACGAATTCTT GGATGTCTTG GGTCAAAGGA TACGTCTGAA GGATCACAAG 
GGCTACAGGG GTGGGCTTGA CATTCAGAAT GGTCACACGG GCGACACAGC TGTCTATGAG 
GTCTTCAAGG AGCGAGAGAT CATGTTCCAT GTATCCACAC TATTGCCGCA CACCGAAGGC 
GATCCACAGC AGCTCCAGAG GAAGCGACAC ATTGGCAATG ACATTGTGGC CATCGTTTTT 
CAGGAGACTA ACACTCCATT CTCACCCGAC ATGATCGCCA GTCACTTTCT GCACGCTTTC 
ATCGTGGTCC AGCCCATTGA GCCGAATACA CCACATACCC GGTACAAGGT CAGCGTAACC 
GCCAGGGATG ATGTTCCCTT CTTTGGTCCC ACTCTGCCGA ATCCCGCAGT TTTCCGCAAG 
GGACAGGAGT TCAAGGAGTT CATCTTGACC AAGCTGATCA ATGCTGAGAA TGCGTGCTAC 
AAAGCTGAGA AGTTTGCAAA GTTGGAACAG CGCACCAGGA CCTCTCTGCT CCAGAATTTA 
TGCGAGGAAC TGCGGGAGAA GACTCGCGAC TTTCTGGGAA CCGATCTTTC CCAGACGTCG 
GCAGGAAGTC CTACACCCGA GACCCCGAAA GCAGAAAGTG GTAGTGGCGG CAATGCCGGC 
TCCCGCTTTA TCGATACGGT GAAGAAGGCT TTAATCATGA GGGTGCGCTC ACAGAGCGTG 
GACACCGGAA ATGGTCATGG ACCTGGACAA ACGGATAAAT TCAGTGTGAA CACGAAGCTG 
GGCACATTGA ATTCCAAGAA GGAGACCCAT CCCGAAATGC AGACACCCAA TCTAAATACC 
TGCAGTCGCA CGGCCTCCAA GTCCTCATCC AAGTCGAAGT CATCGAACGA GTCCACTTCC 
TCCTCCCCGG ACATTACCTC TCGCCAGGCG AACAACAACA ATACCAATTC TATAGGTGGA 
CACCTCCCCC AAAATGGAAC CGGCGGGGGC GTGGTAGCAG CGGCTGTGAG TGCCGCCCAA 
AACGGAGTGG TCGCAGTGGC CACCATGTCG GAAACGAGCG ATGATTCCAG TCTGAATAGC 
GTGGATCTCG ATCCAATGAT GGCGCACTTG GACGGCGGAG CCACCTACAT TGACAGTGAT 
ACTGGGCTAG AAAGCATGAG TTCGGCAGAG GCCACCACCA AGGCGTGCTC CCTGTGCTTA 
GATGGTGTCC AGTCCACCAT AATGGGTAGT TCCCCCAGCA ACGAGACGAT CGTGAAGATC 
GAGAATCTGC GCCAGGAGGT CACACGTTTG AAGTGCGACA AGCTGGATCT ACTCAGACAG 
AATGTGACCT GTCAGCGCGA CATCAAGCGA CTTCGGGAGC GGGAACTTTC CCTTCAAGGC 
GATCTATCGG CAGCTGGCAG AGAAATCCTA CGACTGCGGG ATCTCCTCAA GGAATATATG 
CCCGATACAG CTTCAGCACC ACTCTCCATA TCGCCCATCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RapGAP1 ( NM_001273279.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260208.1 Drosophila melanogaster Rap GTPase activating protein 1 (RapGAP1), transcript variant I, mRNA.

Price & Availability↑

CloneID	OFa14043
Clone ID Related Accession (Same CDS sequence)	NM_144299.3 , NM_001273279.1
Accession Version	NM_001273279.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rap GTPase activating protein 1, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGAAGA TACACATGCT GGAGCAGGTC GTCAACACGG AAAATGTCTG CGAGAATGCC 
CAGGAGCTAT CACCAAAGCG CAAATTGGCG GGTCAGGGGC AAGTGGGTGG TGGCAGCTCC 
TCCTCCAACG GAGGCACGCC CACCGTCTCG GCCACCTCGT CGCCCCACCA CCAGCCAACG 
CCCCCCCAGC GGCACATTCA CACGGGCGGT GGCAAGAGCG CCAGCCAAGG ATCATCACCA 
GCTGGACGTC AGCATGCCTT TATGGCCAAC ACACAGAAGC AGCAACCGGC GGGCGGAGGA 
AGTGGCTCTG GCAGCGTCAC CCACAGTCCG GAACGCCTGC GCGGAGCAAC CACAGCCACC 
CAGGATCTCT TCGAGCTGCT CGAGCGTGTG CAGTGCTCGC GACTCGACGA CCAACGCTGC 
GTCCTGCCCG CATACTTTTC TCAGAACCAC CATCGCGGCA GTAATGCGAG TGAGGAGCGC 
GTGCCCCAGC CACACTCACC CCACGACAGT CACGCCCATC CGCACAACGG TGGGCTGCAG 
CACTCGGGAT CGCGGGCAAC ACTGCGCCAC TCGATGAATC ACTCGAGCAG CTCCACAGTC 
TCGGGCTCCG CCTGCAGCTC GACGCCCGCC TCCCCGCAAC TGAGTGGGGT GGTCAGCGCC 
TCTGGATGCC AGCTGCTGTC CAACGGACAC CACTACCAAT CTCAGTCGTC GGTGAGCTCC 
AACTCATCGA TCGTTTCGGC CATTCCCGCC TCGCAGAGAT TGCTGGAGGA GGCGCTGGCC 
AAACCAGCAC CGTACCCCAT GATCTTGCTG CCACTGCACG GTGGTTACTG GATGGATGGC 
ACCGAACATG AGTGCGGCTA CGATGCGAGA GGAAATCCTC TGCTACCACA GACTACCTGG 
ATGGCAAAGT TCGAGACGGA CGATACGGCC AAGTGCTACA GGAGATTCTA TGCCGCCAGG 
GAGCACTCGA ATTTGATTGG ACTGGATGAG CAGTTGGGAC CGATTTTGCT TTCTATTAAA 
ACGGAAAATG TAGCCAACCA GGAGCACATG CGAATCCTGA TGAGATTGAG AACTGGTACG 
ATGCATGAGC TTCTACCGGT ATCATGCCTA TTACCACAGC CAAGTCCGGC AAAAATGGCG 
CATACACTGA ACGAAAATAT CACAGTCGAG CATTTCATGC CGATTTTGTG CCCCAAGGCC 
TCGCAACTCA TCAGTGTATA CGACGAGCAT GTTCTCGTTT CGCACTTCAA GTTTGGAGTG 
CTCTACCAAA GATATGGACA GACCACCGAG GAGGAACTTT TCGGTAACCA GCAGACATCA 
CCAGCTTTCG ACGAATTCTT GGATGTCTTG GGTCAAAGGA TACGTCTGAA GGATCACAAG 
GGCTACAGGG GTGGGCTTGA CATTCAGAAT GGTCACACGG GCGACACAGC TGTCTATGAG 
GTCTTCAAGG AGCGAGAGAT CATGTTCCAT GTATCCACAC TATTGCCGCA CACCGAAGGC 
GATCCACAGC AGCTCCAGAG GAAGCGACAC ATTGGCAATG ACATTGTGGC CATCGTTTTT 
CAGGAGACTA ACACTCCATT CTCACCCGAC ATGATCGCCA GTCACTTTCT GCACGCTTTC 
ATCGTGGTCC AGCCCATTGA GCCGAATACA CCACATACCC GGTACAAGGT CAGCGTAACC 
GCCAGGGATG ATGTTCCCTT CTTTGGTCCC ACTCTGCCGA ATCCCGCAGT TTTCCGCAAG 
GGACAGGAGT TCAAGGAGTT CATCTTGACC AAGCTGATCA ATGCTGAGAA TGCGTGCTAC 
AAAGCTGAGA AGTTTGCAAA GTTGGAACAG CGCACCAGGA CCTCTCTGCT CCAGAATTTA 
TGCGAGGAAC TGCGGGAGAA GACTCGCGAC TTTCTGGGAA CCGATCTTTC CCAGACGTCG 
GCAGGAAGTC CTACACCCGA GACCCCGAAA GCAGAAAGTG GTAGTGGCGG CAATGCCGGC 
TCCCGCTTTA TCGATACGGT GAAGAAGGCT TTAATCATGA GGGTGCGCTC ACAGAGCGTG 
GACACCGGAA ATGGTCATGG ACCTGGACAA ACGGATAAAT TCAGTGTGAA CACGAAGCTG 
GGCACATTGA ATTCCAAGAA GGAGACCCAT CCCGAAATGC AGACACCCAA TCTAAATACC 
TGCAGTCGCA CGGCCTCCAA GTCCTCATCC AAGTCGAAGT CATCGAACGA GTCCACTTCC 
TCCTCCCCGG ACATTACCTC TCGCCAGGCG AACAACAACA ATACCAATTC TATAGGTGGA 
CACCTCCCCC AAAATGGAAC CGGCGGGGGC GTGGTAGCAG CGGCTGTGAG TGCCGCCCAA 
AACGGAGTGG TCGCAGTGGC CACCATGTCG GAAACGAGCG ATGATTCCAG TCTGAATAGC 
GTGGATCTCG ATCCAATGAT GGCGCACTTG GACGGCGGAG CCACCTACAT TGACAGTGAT 
ACTGGGCTAG AAAGCATGAG TTCGGCAGAG GCCACCACCA AGGCGTGCTC CCTGTGCTTA 
GATGGTGTCC AGTCCACCAT AATGGGTAGT TCCCCCAGCA ACGAGACGAT CGTGAAGATC 
GAGAATCTGC GCCAGGAGGT CACACGTTTG AAGTGCGACA AGCTGGATCT ACTCAGACAG 
AATGTGACCT GTCAGCGCGA CATCAAGCGA CTTCGGGAGC GGGAACTTTC CCTTCAAGGC 
GATCTATCGG CAGCTGGCAG AGAAATCCTA CGACTGCGGG ATCTCCTCAA GGAATATATG 
CCCGATACAG CTTCAGCACC ACTCTCCATA TCGCCCATCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260208.1
CDS	441..3242
Translation	MLKIHMLEQVVNTENVCENAQELSPKRKLAGQGQVGGGSSSSNGGTPTVSATSSPHHQPTPPQRHIHTGGGKSASQGSSPAGRQHAFMANTQKQQPAGGGSGSGSVTHSPERLRGATTATQDLFELLERVQCSRLDDQRCVLPAYFSQNHHRGSNASEERVPQPHSPHDSHAHPHNGGLQHSGSRATLRHSMNHSSSSTVSGSACSSTPASPQLSGVVSASGCQLLSNGHHYQSQSSVSSNSSIVSAIPASQRLLEEALAKPAPYPMILLPLHGGYWMDGTEHECGYDARGNPLLPQTTWMAKFETDDTAKCYRRFYAAREHSNLIGLDEQLGPILLSIKTENVANQEHMRILMRLRTGTMHELLPVSCLLPQPSPAKMAHTLNENITVEHFMPILCPKASQLISVYDEHVLVSHFKFGVLYQRYGQTTEEELFGNQQTSPAFDEFLDVLGQRIRLKDHKGYRGGLDIQNGHTGDTAVYEVFKEREIMFHVSTLLPHTEGDPQQLQRKRHIGNDIVAIVFQETNTPFSPDMIASHFLHAFIVVQPIEPNTPHTRYKVSVTARDDVPFFGPTLPNPAVFRKGQEFKEFILTKLINAENACYKAEKFAKLEQRTRTSLLQNLCEELREKTRDFLGTDLSQTSAGSPTPETPKAESGSGGNAGSRFIDTVKKALIMRVRSQSVDTGNGHGPGQTDKFSVNTKLGTLNSKKETHPEMQTPNLNTCSRTASKSSSKSKSSNESTSSSPDITSRQANNNNTNSIGGHLPQNGTGGGVVAAAVSAAQNGVVAVATMSETSDDSSLNSVDLDPMMAHLDGGATYIDSDTGLESMSSAEATTKACSLCLDGVQSTIMGSSPSNETIVKIENLRQEVTRLKCDKLDLLRQNVTCQRDIKRLRERELSLQGDLSAAGREILRLRDLLKEYMPDTASAPLSISPI

Target ORF information:

RefSeq Version	NM_001273279.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rap GTPase activating protein 1 (RapGAP1), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273279.1

ORF Insert Sequence:

ATGCTGAAGA TACACATGCT GGAGCAGGTC GTCAACACGG AAAATGTCTG CGAGAATGCC 
CAGGAGCTAT CACCAAAGCG CAAATTGGCG GGTCAGGGGC AAGTGGGTGG TGGCAGCTCC 
TCCTCCAACG GAGGCACGCC CACCGTCTCG GCCACCTCGT CGCCCCACCA CCAGCCAACG 
CCCCCCCAGC GGCACATTCA CACGGGCGGT GGCAAGAGCG CCAGCCAAGG ATCATCACCA 
GCTGGACGTC AGCATGCCTT TATGGCCAAC ACACAGAAGC AGCAACCGGC GGGCGGAGGA 
AGTGGCTCTG GCAGCGTCAC CCACAGTCCG GAACGCCTGC GCGGAGCAAC CACAGCCACC 
CAGGATCTCT TCGAGCTGCT CGAGCGTGTG CAGTGCTCGC GACTCGACGA CCAACGCTGC 
GTCCTGCCCG CATACTTTTC TCAGAACCAC CATCGCGGCA GTAATGCGAG TGAGGAGCGC 
GTGCCCCAGC CACACTCACC CCACGACAGT CACGCCCATC CGCACAACGG TGGGCTGCAG 
CACTCGGGAT CGCGGGCAAC ACTGCGCCAC TCGATGAATC ACTCGAGCAG CTCCACAGTC 
TCGGGCTCCG CCTGCAGCTC GACGCCCGCC TCCCCGCAAC TGAGTGGGGT GGTCAGCGCC 
TCTGGATGCC AGCTGCTGTC CAACGGACAC CACTACCAAT CTCAGTCGTC GGTGAGCTCC 
AACTCATCGA TCGTTTCGGC CATTCCCGCC TCGCAGAGAT TGCTGGAGGA GGCGCTGGCC 
AAACCAGCAC CGTACCCCAT GATCTTGCTG CCACTGCACG GTGGTTACTG GATGGATGGC 
ACCGAACATG AGTGCGGCTA CGATGCGAGA GGAAATCCTC TGCTACCACA GACTACCTGG 
ATGGCAAAGT TCGAGACGGA CGATACGGCC AAGTGCTACA GGAGATTCTA TGCCGCCAGG 
GAGCACTCGA ATTTGATTGG ACTGGATGAG CAGTTGGGAC CGATTTTGCT TTCTATTAAA 
ACGGAAAATG TAGCCAACCA GGAGCACATG CGAATCCTGA TGAGATTGAG AACTGGTACG 
ATGCATGAGC TTCTACCGGT ATCATGCCTA TTACCACAGC CAAGTCCGGC AAAAATGGCG 
CATACACTGA ACGAAAATAT CACAGTCGAG CATTTCATGC CGATTTTGTG CCCCAAGGCC 
TCGCAACTCA TCAGTGTATA CGACGAGCAT GTTCTCGTTT CGCACTTCAA GTTTGGAGTG 
CTCTACCAAA GATATGGACA GACCACCGAG GAGGAACTTT TCGGTAACCA GCAGACATCA 
CCAGCTTTCG ACGAATTCTT GGATGTCTTG GGTCAAAGGA TACGTCTGAA GGATCACAAG 
GGCTACAGGG GTGGGCTTGA CATTCAGAAT GGTCACACGG GCGACACAGC TGTCTATGAG 
GTCTTCAAGG AGCGAGAGAT CATGTTCCAT GTATCCACAC TATTGCCGCA CACCGAAGGC 
GATCCACAGC AGCTCCAGAG GAAGCGACAC ATTGGCAATG ACATTGTGGC CATCGTTTTT 
CAGGAGACTA ACACTCCATT CTCACCCGAC ATGATCGCCA GTCACTTTCT GCACGCTTTC 
ATCGTGGTCC AGCCCATTGA GCCGAATACA CCACATACCC GGTACAAGGT CAGCGTAACC 
GCCAGGGATG ATGTTCCCTT CTTTGGTCCC ACTCTGCCGA ATCCCGCAGT TTTCCGCAAG 
GGACAGGAGT TCAAGGAGTT CATCTTGACC AAGCTGATCA ATGCTGAGAA TGCGTGCTAC 
AAAGCTGAGA AGTTTGCAAA GTTGGAACAG CGCACCAGGA CCTCTCTGCT CCAGAATTTA 
TGCGAGGAAC TGCGGGAGAA GACTCGCGAC TTTCTGGGAA CCGATCTTTC CCAGACGTCG 
GCAGGAAGTC CTACACCCGA GACCCCGAAA GCAGAAAGTG GTAGTGGCGG CAATGCCGGC 
TCCCGCTTTA TCGATACGGT GAAGAAGGCT TTAATCATGA GGGTGCGCTC ACAGAGCGTG 
GACACCGGAA ATGGTCATGG ACCTGGACAA ACGGATAAAT TCAGTGTGAA CACGAAGCTG 
GGCACATTGA ATTCCAAGAA GGAGACCCAT CCCGAAATGC AGACACCCAA TCTAAATACC 
TGCAGTCGCA CGGCCTCCAA GTCCTCATCC AAGTCGAAGT CATCGAACGA GTCCACTTCC 
TCCTCCCCGG ACATTACCTC TCGCCAGGCG AACAACAACA ATACCAATTC TATAGGTGGA 
CACCTCCCCC AAAATGGAAC CGGCGGGGGC GTGGTAGCAG CGGCTGTGAG TGCCGCCCAA 
AACGGAGTGG TCGCAGTGGC CACCATGTCG GAAACGAGCG ATGATTCCAG TCTGAATAGC 
GTGGATCTCG ATCCAATGAT GGCGCACTTG GACGGCGGAG CCACCTACAT TGACAGTGAT 
ACTGGGCTAG AAAGCATGAG TTCGGCAGAG GCCACCACCA AGGCGTGCTC CCTGTGCTTA 
GATGGTGTCC AGTCCACCAT AATGGGTAGT TCCCCCAGCA ACGAGACGAT CGTGAAGATC 
GAGAATCTGC GCCAGGAGGT CACACGTTTG AAGTGCGACA AGCTGGATCT ACTCAGACAG 
AATGTGACCT GTCAGCGCGA CATCAAGCGA CTTCGGGAGC GGGAACTTTC CCTTCAAGGC 
GATCTATCGG CAGCTGGCAG AGAAATCCTA CGACTGCGGG ATCTCCTCAA GGAATATATG 
CCCGATACAG CTTCAGCACC ACTCTCCATA TCGCCCATCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RapGAP1 ( NM_001103644.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097114.2 Drosophila melanogaster Rap GTPase activating protein 1 (RapGAP1), transcript variant H, mRNA.

Price & Availability↑

CloneID	OFa16368
Clone ID Related Accession (Same CDS sequence)	NM_001103644.3
Accession Version	NM_001103644.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rap GTPase activating protein 1, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001103644.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTTCCT ACAGGACATT GGTGGATCAT GGCCATCCGA TTATAGTGGG AAGCAGTGAA 
ATATCGCTGG CCCCGAGTTC GGCAGCCAGT TCGCCCAAGC CCCTACACCG GATGATCAAG 
TACTGGCGCA ACAGTTCCGG AAAAATTCCG GGTCTCCGCA AAAGCGAGAG TTTCGCCGAG 
TATCGTCGCC ATTCATCCAA CTCGGCCACA ATTTCGGGAG GATCAGGGGG CAGATCGAGC 
ACTTCGAGTG CCAGGCAATT GCAATACCAG CGACTGGAGA TGGAAAGCTG CGAGAATATA 
GATATGCTGA CAGAACCACT AAGACGTCAG CATGCCTTTA TGGCCAACAC ACAGAAGCAG 
CAACCGGCGG GCGGAGGAAG TGGCTCTGGC AGCGTCACCC ACAGTCCGGA ACGCCTGCGC 
GGAGCAACCA CAGCCACCCA GGATCTCTTC GAGCTGCTCG AGCGTGTGCA GTGCTCGCGA 
CTCGACGACC AACGCTGCGT CCTGCCCGCA TACTTTTCTC AGAACCACCA TCGCGGCAGT 
AATGCGAGTG AGGAGCGCGT GCCCCAGCCA CACTCACCCC ACGACAGTCA CGCCCATCCG 
CACAACGGTG GGCTGCAGCA CTCGGGATCG CGGGCAACAC TGCGCCACTC GATGAATCAC 
TCGAGCAGCT CCACAGTCTC GGGCTCCGCC TGCAGCTCGA CGCCCGCCTC CCCGCAACTG 
AGTGGGGTGG TCAGCGCCTC TGGATGCCAG CTGCTGTCCA ACGGACACCA CTACCAATCT 
CAGTCGTCGG TGAGCTCCAA CTCATCGATC GTTTCGGCCA TTCCCGCCTC GCAGAGATTG 
CTGGAGGAGG CGCTGGCCAA ACCAGCACCG TACCCCATGA TCTTGCTGCC ACTGCACGGT 
GGTTACTGGA TGGATGGCAC CGAACATGAG TGCGGCTACG ATGCGAGAGG AAATCCTCTG 
CTACCACAGA CTACCTGGAT GGCAAAGTTC GAGACGGACG ATACGGCCAA GTGCTACAGG 
AGATTCTATG CCGCCAGGGA GCACTCGAAT TTGATTGGAC TGGATGAGCA GTTGGGACCG 
ATTTTGCTTT CTATTAAAAC GGAAAATGTA GCCAACCAGG AGCACATGCG AATCCTGATG 
AGATTGAGAA CTGGTACGAT GCATGAGCTT CTACCGGTAT CATGCCTATT ACCACAGCCA 
AGTCCGGCAA AAATGGCGCA TACACTGAAC GAAAATATCA CAGTCGAGCA TTTCATGCCG 
ATTTTGTGCC CCAAGGCCTC GCAACTCATC AGTGTATACG ACGAGCATGT TCTCGTTTCG 
CACTTCAAGT TTGGAGTGCT CTACCAAAGA TATGGACAGA CCACCGAGGA GGAACTTTTC 
GGTAACCAGC AGACATCACC AGCTTTCGAC GAATTCTTGG ATGTCTTGGG TCAAAGGATA 
CGTCTGAAGG ATCACAAGGG CTACAGGGGT GGGCTTGACA TTCAGAATGG TCACACGGGC 
GACACAGCTG TCTATGAGGT CTTCAAGGAG CGAGAGATCA TGTTCCATGT ATCCACACTA 
TTGCCGCACA CCGAAGGCGA TCCACAGCAG CTCCAGAGGA AGCGACACAT TGGCAATGAC 
ATTGTGGCCA TCGTTTTTCA GGAGACTAAC ACTCCATTCT CACCCGACAT GATCGCCAGT 
CACTTTCTGC ACGCTTTCAT CGTGGTCCAG CCCATTGAGC CGAATACACC ACATACCCGG 
TACAAGGTCA GCGTAACCGC CAGGGATGAT GTTCCCTTCT TTGGTCCCAC TCTGCCGAAT 
CCCGCAGTTT TCCGCAAGGG ACAGGAGTTC AAGGAGTTCA TCTTGACCAA GCTGATCAAT 
GCTGAGAATG CGTGCTACAA AGCTGAGAAG TTTGCAAAGT TGGAACAGCG CACCAGGACC 
TCTCTGCTCC AGAATTTATG CGAGGAACTG CGGGAGAAGA CTCGCGACTT TCTGGGAACC 
GATCTTTCCC AGACGTCGGC AGGAAGTCCT ACACCCGAGA CCCCGAAAGC AGAAAGTGGT 
AGTGGCGGCA ATGCCGGCTC CCGCTTTATC GATACGGTGA AGAAGGCTTT AATCATGAGG 
GTGCGCTCAC AGAGCGTGGA CACCGGAAAT GGTCATGGAC CTGGACAAAC GGATAAATTC 
AGTGTGAACA CGAAGCTGGG CACATTGAAT TCCAAGAAGG AGACCCATCC CGAAATGCAG 
ACACCCAATC TAAATACCTG CAGTCGCACG GCCTCCAAGT CCTCATCCAA GTCGAAGTCA 
TCGAACGAGT CCACTTCCTC CTCCCCGGAC ATTACCTCTC GCCAGGCGAA CAACAACAAT 
ACCAATTCTA TAGGTGGACA CCTCCCCCAA AATGGAACCG GCGGGGGCGT GGTAGCAGCG 
GCTGTGAGTG CCGCCCAAAA CGGAGTGGTC GCAGTGGCCA CCATGTCGGA AACGAGCGAT 
GATTCCAGTC TGAATAGCGT GGATCTCGAT CCAATGATGG CGCACTTGGA CGGCGGAGCC 
ACCTACATTG ACAGTGATAC TGGGCTAGAA AGCATGAGTT CGGCAGAGGC CACCACCAAG 
GCGTGCTCCC TGTGCTTAGA TGGTGTCCAG TCCACCATAA TGGGTAGTTC CCCCAGCAAC 
GAGACGATCG TGAAGATCGA GAATCTGCGC CAGGAGGTCA CACGTTTGAA GTGCGACAAG 
CTGGATCTAC TCAGACAGAA TGTGACCTGT CAGCGCGACA TCAAGCGACT TCGGGAGCGG 
GAACTTTCCC TTCAAGGCGA TCTATCGGCA GCTGGCAGAG AAATCCTACG ACTGCGGGAT 
CTCCTCAAGG AATATATGCC CGATACAGCT TCAGCACCAC TCTCCATATC GCCCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097114.2
CDS	422..3301
Translation	MSSYRTLVDHGHPIIVGSSEISLAPSSAASSPKPLHRMIKYWRNSSGKIPGLRKSESFAEYRRHSSNSATISGGSGGRSSTSSARQLQYQRLEMESCENIDMLTEPLRRQHAFMANTQKQQPAGGGSGSGSVTHSPERLRGATTATQDLFELLERVQCSRLDDQRCVLPAYFSQNHHRGSNASEERVPQPHSPHDSHAHPHNGGLQHSGSRATLRHSMNHSSSSTVSGSACSSTPASPQLSGVVSASGCQLLSNGHHYQSQSSVSSNSSIVSAIPASQRLLEEALAKPAPYPMILLPLHGGYWMDGTEHECGYDARGNPLLPQTTWMAKFETDDTAKCYRRFYAAREHSNLIGLDEQLGPILLSIKTENVANQEHMRILMRLRTGTMHELLPVSCLLPQPSPAKMAHTLNENITVEHFMPILCPKASQLISVYDEHVLVSHFKFGVLYQRYGQTTEEELFGNQQTSPAFDEFLDVLGQRIRLKDHKGYRGGLDIQNGHTGDTAVYEVFKEREIMFHVSTLLPHTEGDPQQLQRKRHIGNDIVAIVFQETNTPFSPDMIASHFLHAFIVVQPIEPNTPHTRYKVSVTARDDVPFFGPTLPNPAVFRKGQEFKEFILTKLINAENACYKAEKFAKLEQRTRTSLLQNLCEELREKTRDFLGTDLSQTSAGSPTPETPKAESGSGGNAGSRFIDTVKKALIMRVRSQSVDTGNGHGPGQTDKFSVNTKLGTLNSKKETHPEMQTPNLNTCSRTASKSSSKSKSSNESTSSSPDITSRQANNNNTNSIGGHLPQNGTGGGVVAAAVSAAQNGVVAVATMSETSDDSSLNSVDLDPMMAHLDGGATYIDSDTGLESMSSAEATTKACSLCLDGVQSTIMGSSPSNETIVKIENLRQEVTRLKCDKLDLLRQNVTCQRDIKRLRERELSLQGDLSAAGREILRLRDLLKEYMPDTASAPLSISPI

Target ORF information:

RefSeq Version	NM_001103644.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rap GTPase activating protein 1 (RapGAP1), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103644.3

ORF Insert Sequence:

ATGAGTTCCT ACAGGACATT GGTGGATCAT GGCCATCCGA TTATAGTGGG AAGCAGTGAA 
ATATCGCTGG CCCCGAGTTC GGCAGCCAGT TCGCCCAAGC CCCTACACCG GATGATCAAG 
TACTGGCGCA ACAGTTCCGG AAAAATTCCG GGTCTCCGCA AAAGCGAGAG TTTCGCCGAG 
TATCGTCGCC ATTCATCCAA CTCGGCCACA ATTTCGGGAG GATCAGGGGG CAGATCGAGC 
ACTTCGAGTG CCAGGCAATT GCAATACCAG CGACTGGAGA TGGAAAGCTG CGAGAATATA 
GATATGCTGA CAGAACCACT AAGACGTCAG CATGCCTTTA TGGCCAACAC ACAGAAGCAG 
CAACCGGCGG GCGGAGGAAG TGGCTCTGGC AGCGTCACCC ACAGTCCGGA ACGCCTGCGC 
GGAGCAACCA CAGCCACCCA GGATCTCTTC GAGCTGCTCG AGCGTGTGCA GTGCTCGCGA 
CTCGACGACC AACGCTGCGT CCTGCCCGCA TACTTTTCTC AGAACCACCA TCGCGGCAGT 
AATGCGAGTG AGGAGCGCGT GCCCCAGCCA CACTCACCCC ACGACAGTCA CGCCCATCCG 
CACAACGGTG GGCTGCAGCA CTCGGGATCG CGGGCAACAC TGCGCCACTC GATGAATCAC 
TCGAGCAGCT CCACAGTCTC GGGCTCCGCC TGCAGCTCGA CGCCCGCCTC CCCGCAACTG 
AGTGGGGTGG TCAGCGCCTC TGGATGCCAG CTGCTGTCCA ACGGACACCA CTACCAATCT 
CAGTCGTCGG TGAGCTCCAA CTCATCGATC GTTTCGGCCA TTCCCGCCTC GCAGAGATTG 
CTGGAGGAGG CGCTGGCCAA ACCAGCACCG TACCCCATGA TCTTGCTGCC ACTGCACGGT 
GGTTACTGGA TGGATGGCAC CGAACATGAG TGCGGCTACG ATGCGAGAGG AAATCCTCTG 
CTACCACAGA CTACCTGGAT GGCAAAGTTC GAGACGGACG ATACGGCCAA GTGCTACAGG 
AGATTCTATG CCGCCAGGGA GCACTCGAAT TTGATTGGAC TGGATGAGCA GTTGGGACCG 
ATTTTGCTTT CTATTAAAAC GGAAAATGTA GCCAACCAGG AGCACATGCG AATCCTGATG 
AGATTGAGAA CTGGTACGAT GCATGAGCTT CTACCGGTAT CATGCCTATT ACCACAGCCA 
AGTCCGGCAA AAATGGCGCA TACACTGAAC GAAAATATCA CAGTCGAGCA TTTCATGCCG 
ATTTTGTGCC CCAAGGCCTC GCAACTCATC AGTGTATACG ACGAGCATGT TCTCGTTTCG 
CACTTCAAGT TTGGAGTGCT CTACCAAAGA TATGGACAGA CCACCGAGGA GGAACTTTTC 
GGTAACCAGC AGACATCACC AGCTTTCGAC GAATTCTTGG ATGTCTTGGG TCAAAGGATA 
CGTCTGAAGG ATCACAAGGG CTACAGGGGT GGGCTTGACA TTCAGAATGG TCACACGGGC 
GACACAGCTG TCTATGAGGT CTTCAAGGAG CGAGAGATCA TGTTCCATGT ATCCACACTA 
TTGCCGCACA CCGAAGGCGA TCCACAGCAG CTCCAGAGGA AGCGACACAT TGGCAATGAC 
ATTGTGGCCA TCGTTTTTCA GGAGACTAAC ACTCCATTCT CACCCGACAT GATCGCCAGT 
CACTTTCTGC ACGCTTTCAT CGTGGTCCAG CCCATTGAGC CGAATACACC ACATACCCGG 
TACAAGGTCA GCGTAACCGC CAGGGATGAT GTTCCCTTCT TTGGTCCCAC TCTGCCGAAT 
CCCGCAGTTT TCCGCAAGGG ACAGGAGTTC AAGGAGTTCA TCTTGACCAA GCTGATCAAT 
GCTGAGAATG CGTGCTACAA AGCTGAGAAG TTTGCAAAGT TGGAACAGCG CACCAGGACC 
TCTCTGCTCC AGAATTTATG CGAGGAACTG CGGGAGAAGA CTCGCGACTT TCTGGGAACC 
GATCTTTCCC AGACGTCGGC AGGAAGTCCT ACACCCGAGA CCCCGAAAGC AGAAAGTGGT 
AGTGGCGGCA ATGCCGGCTC CCGCTTTATC GATACGGTGA AGAAGGCTTT AATCATGAGG 
GTGCGCTCAC AGAGCGTGGA CACCGGAAAT GGTCATGGAC CTGGACAAAC GGATAAATTC 
AGTGTGAACA CGAAGCTGGG CACATTGAAT TCCAAGAAGG AGACCCATCC CGAAATGCAG 
ACACCCAATC TAAATACCTG CAGTCGCACG GCCTCCAAGT CCTCATCCAA GTCGAAGTCA 
TCGAACGAGT CCACTTCCTC CTCCCCGGAC ATTACCTCTC GCCAGGCGAA CAACAACAAT 
ACCAATTCTA TAGGTGGACA CCTCCCCCAA AATGGAACCG GCGGGGGCGT GGTAGCAGCG 
GCTGTGAGTG CCGCCCAAAA CGGAGTGGTC GCAGTGGCCA CCATGTCGGA AACGAGCGAT 
GATTCCAGTC TGAATAGCGT GGATCTCGAT CCAATGATGG CGCACTTGGA CGGCGGAGCC 
ACCTACATTG ACAGTGATAC TGGGCTAGAA AGCATGAGTT CGGCAGAGGC CACCACCAAG 
GCGTGCTCCC TGTGCTTAGA TGGTGTCCAG TCCACCATAA TGGGTAGTTC CCCCAGCAAC 
GAGACGATCG TGAAGATCGA GAATCTGCGC CAGGAGGTCA CACGTTTGAA GTGCGACAAG 
CTGGATCTAC TCAGACAGAA TGTGACCTGT CAGCGCGACA TCAAGCGACT TCGGGAGCGG 
GAACTTTCCC TTCAAGGCGA TCTATCGGCA GCTGGCAGAG AAATCCTACG ACTGCGGGAT 
CTCCTCAAGG AATATATGCC CGATACAGCT TCAGCACCAC TCTCCATATC GCCCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RapGAP1 ( NM_001014479.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014479.1 Drosophila melanogaster Rap GTPase activating protein 1 (RapGAP1), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa16369
Clone ID Related Accession (Same CDS sequence)	NM_001014479.2
Accession Version	NM_001014479.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rap GTPase activating protein 1, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001014479.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAATATT TAACGAATAC ACACAAGGAA TCCTACACAT TGGTGGCACG CAAACGCTAC 
ATGAAGAATC TGCTACGTCA GCATGCCTTT ATGGCCAACA CACAGAAGCA GCAACCGGCG 
GGCGGAGGAA GTGGCTCTGG CAGCGTCACC CACAGTCCGG AACGCCTGCG CGGAGCAACC 
ACAGCCACCC AGGATCTCTT CGAGCTGCTC GAGCGTGTGC AGTGCTCGCG ACTCGACGAC 
CAACGCTGCG TCCTGCCCGC ATACTTTTCT CAGAACCACC ATCGCGGCAG TAATGCGAGT 
GAGGAGCGCG TGCCCCAGCC ACACTCACCC CACGACAGTC ACGCCCATCC GCACAACGGT 
GGGCTGCAGC ACTCGGGATC GCGGGCAACA CTGCGCCACT CGATGAATCA CTCGAGCAGC 
TCCACAGTCT CGGGCTCCGC CTGCAGCTCG ACGCCCGCCT CCCCGCAACT GAGTGGGGTG 
GTCAGCGCCT CTGGATGCCA GCTGCTGTCC AACGGACACC ACTACCAATC TCAGTCGTCG 
GTGAGCTCCA ACTCATCGAT CGTTTCGGCC ATTCCCGCCT CGCAGAGATT GCTGGAGGAG 
GCGCTGGCCA AACCAGCACC GTACCCCATG ATCTTGCTGC CACTGCACGG TGGTTACTGG 
ATGGATGGCA CCGAACATGA GTGCGGCTAC GATGCGAGAG GAAATCCTCT GCTACCACAG 
ACTACCTGGA TGGCAAAGTT CGAGACGGAC GATACGGCCA AGTGCTACAG GAGATTCTAT 
GCCGCCAGGG AGCACTCGAA TTTGATTGGA CTGGATGAGC AGTTGGGACC GATTTTGCTT 
TCTATTAAAA CGGAAAATGT AGCCAACCAG GAGCACATGC GAATCCTGAT GAGATTGAGA 
ACTGGTACGA TGCATGAGCT TCTACCGGTA TCATGCCTAT TACCACAGCC AAGTCCGGCA 
AAAATGGCGC ATACACTGAA CGAAAATATC ACAGTCGAGC ATTTCATGCC GATTTTGTGC 
CCCAAGGCCT CGCAACTCAT CAGTGTATAC GACGAGCATG TTCTCGTTTC GCACTTCAAG 
TTTGGAGTGC TCTACCAAAG ATATGGACAG ACCACCGAGG AGGAACTTTT CGGTAACCAG 
CAGACATCAC CAGCTTTCGA CGAATTCTTG GATGTCTTGG GTCAAAGGAT ACGTCTGAAG 
GATCACAAGG GCTACAGGGG TGGGCTTGAC ATTCAGAATG GTCACACGGG CGACACAGCT 
GTCTATGAGG TCTTCAAGGA GCGAGAGATC ATGTTCCATG TATCCACACT ATTGCCGCAC 
ACCGAAGGCG ATCCACAGCA GCTCCAGAGG AAGCGACACA TTGGCAATGA CATTGTGGCC 
ATCGTTTTTC AGGAGACTAA CACTCCATTC TCACCCGACA TGATCGCCAG TCACTTTCTG 
CACGCTTTCA TCGTGGTCCA GCCCATTGAG CCGAATACAC CACATACCCG GTACAAGGTC 
AGCGTAACCG CCAGGGATGA TGTTCCCTTC TTTGGTCCCA CTCTGCCGAA TCCCGCAGTT 
TTCCGCAAGG GACAGGAGTT CAAGGAGTTC ATCTTGACCA AGCTGATCAA TGCTGAGAAT 
GCGTGCTACA AAGCTGAGAA GTTTGCAAAG TTGGAACAGC GCACCAGGAC CTCTCTGCTC 
CAGAATTTAT GCGAGGAACT GCGGGAGAAG ACTCGCGACT TTCTGGGAAC CGATCTTTCC 
CAGACGTCGG CAGGAAGTCC TACACCCGAG ACCCCGAAAG CAGAAAGTGG TAGTGGCGGC 
AATGCCGGCT CCCGCTTTAT CGATACGGTG AAGAAGGCTT TAATCATGAG GGTGCGCTCA 
CAGAGCGTGG ACACCGGAAA TGGTCATGGA CCTGGACAAA CGGATAAATT CAGTGTGAAC 
ACGAAGCTGG GCACATTGAA TTCCAAGAAG GAGACCCATC CCGAAATGCA GACACCCAAT 
CTAAATACCT GCAGTCGCAC GGCCTCCAAG TCCTCATCCA AGTCGAAGTC ATCGAACGAG 
TCCACTTCCT CCTCCCCGGA CATTACCTCT CGCCAGGCGA ACAACAACAA TACCAATTCT 
ATAGGTGGAC ACCTCCCCCA AAATGGAACC GGCGGGGGCG TGGTAGCAGC GGCTGTGAGT 
GCCGCCCAAA ACGGAGTGGT CGCAGTGGCC ACCATGTCGG AAACGAGCGA TGATTCCAGT 
CTGAATAGCG TGGATCTCGA TCCAATGATG GCGCACTTGG ACGGCGGAGC CACCTACATT 
GACAGTGATA CTGGGCTAGA AAGCATGAGT TCGGCAGAGG CCACCACCAA GGCGTGCTCC 
CTGTGCTTAG ATGGTGTCCA GTCCACCATA ATGGGTAGTT CCCCCAGCAA CGAGACGATC 
GTGAAGATCG AGAATCTGCG CCAGGAGGTC ACACGTTTGA AGTGCGACAA GCTGGATCTA 
CTCAGACAGA ATGTGACCTG TCAGCGCGAC ATCAAGCGAC TTCGGGAGCG GGAACTTTCC 
CTTCAAGGCG ATCTATCGGC AGCTGGCAGA GAAATCCTAC GACTGCGGGA TCTCCTCAAG 
GAATATATGC CCGATACAGC TTCAGCACCA CTCTCCATAT CGCCCATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014479.1
CDS	372..3002
Translation	MKYLTNTHKESYTLVARKRYMKNLLRQHAFMANTQKQQPAGGGSGSGSVTHSPERLRGATTATQDLFELLERVQCSRLDDQRCVLPAYFSQNHHRGSNASEERVPQPHSPHDSHAHPHNGGLQHSGSRATLRHSMNHSSSSTVSGSACSSTPASPQLSGVVSASGCQLLSNGHHYQSQSSVSSNSSIVSAIPASQRLLEEALAKPAPYPMILLPLHGGYWMDGTEHECGYDARGNPLLPQTTWMAKFETDDTAKCYRRFYAAREHSNLIGLDEQLGPILLSIKTENVANQEHMRILMRLRTGTMHELLPVSCLLPQPSPAKMAHTLNENITVEHFMPILCPKASQLISVYDEHVLVSHFKFGVLYQRYGQTTEEELFGNQQTSPAFDEFLDVLGQRIRLKDHKGYRGGLDIQNGHTGDTAVYEVFKEREIMFHVSTLLPHTEGDPQQLQRKRHIGNDIVAIVFQETNTPFSPDMIASHFLHAFIVVQPIEPNTPHTRYKVSVTARDDVPFFGPTLPNPAVFRKGQEFKEFILTKLINAENACYKAEKFAKLEQRTRTSLLQNLCEELREKTRDFLGTDLSQTSAGSPTPETPKAESGSGGNAGSRFIDTVKKALIMRVRSQSVDTGNGHGPGQTDKFSVNTKLGTLNSKKETHPEMQTPNLNTCSRTASKSSSKSKSSNESTSSSPDITSRQANNNNTNSIGGHLPQNGTGGGVVAAAVSAAQNGVVAVATMSETSDDSSLNSVDLDPMMAHLDGGATYIDSDTGLESMSSAEATTKACSLCLDGVQSTIMGSSPSNETIVKIENLRQEVTRLKCDKLDLLRQNVTCQRDIKRLRERELSLQGDLSAAGREILRLRDLLKEYMPDTASAPLSISPI

Target ORF information:

RefSeq Version	NM_001014479.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rap GTPase activating protein 1 (RapGAP1), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014479.2

ORF Insert Sequence:

ATGAAATATT TAACGAATAC ACACAAGGAA TCCTACACAT TGGTGGCACG CAAACGCTAC 
ATGAAGAATC TGCTACGTCA GCATGCCTTT ATGGCCAACA CACAGAAGCA GCAACCGGCG 
GGCGGAGGAA GTGGCTCTGG CAGCGTCACC CACAGTCCGG AACGCCTGCG CGGAGCAACC 
ACAGCCACCC AGGATCTCTT CGAGCTGCTC GAGCGTGTGC AGTGCTCGCG ACTCGACGAC 
CAACGCTGCG TCCTGCCCGC ATACTTTTCT CAGAACCACC ATCGCGGCAG TAATGCGAGT 
GAGGAGCGCG TGCCCCAGCC ACACTCACCC CACGACAGTC ACGCCCATCC GCACAACGGT 
GGGCTGCAGC ACTCGGGATC GCGGGCAACA CTGCGCCACT CGATGAATCA CTCGAGCAGC 
TCCACAGTCT CGGGCTCCGC CTGCAGCTCG ACGCCCGCCT CCCCGCAACT GAGTGGGGTG 
GTCAGCGCCT CTGGATGCCA GCTGCTGTCC AACGGACACC ACTACCAATC TCAGTCGTCG 
GTGAGCTCCA ACTCATCGAT CGTTTCGGCC ATTCCCGCCT CGCAGAGATT GCTGGAGGAG 
GCGCTGGCCA AACCAGCACC GTACCCCATG ATCTTGCTGC CACTGCACGG TGGTTACTGG 
ATGGATGGCA CCGAACATGA GTGCGGCTAC GATGCGAGAG GAAATCCTCT GCTACCACAG 
ACTACCTGGA TGGCAAAGTT CGAGACGGAC GATACGGCCA AGTGCTACAG GAGATTCTAT 
GCCGCCAGGG AGCACTCGAA TTTGATTGGA CTGGATGAGC AGTTGGGACC GATTTTGCTT 
TCTATTAAAA CGGAAAATGT AGCCAACCAG GAGCACATGC GAATCCTGAT GAGATTGAGA 
ACTGGTACGA TGCATGAGCT TCTACCGGTA TCATGCCTAT TACCACAGCC AAGTCCGGCA 
AAAATGGCGC ATACACTGAA CGAAAATATC ACAGTCGAGC ATTTCATGCC GATTTTGTGC 
CCCAAGGCCT CGCAACTCAT CAGTGTATAC GACGAGCATG TTCTCGTTTC GCACTTCAAG 
TTTGGAGTGC TCTACCAAAG ATATGGACAG ACCACCGAGG AGGAACTTTT CGGTAACCAG 
CAGACATCAC CAGCTTTCGA CGAATTCTTG GATGTCTTGG GTCAAAGGAT ACGTCTGAAG 
GATCACAAGG GCTACAGGGG TGGGCTTGAC ATTCAGAATG GTCACACGGG CGACACAGCT 
GTCTATGAGG TCTTCAAGGA GCGAGAGATC ATGTTCCATG TATCCACACT ATTGCCGCAC 
ACCGAAGGCG ATCCACAGCA GCTCCAGAGG AAGCGACACA TTGGCAATGA CATTGTGGCC 
ATCGTTTTTC AGGAGACTAA CACTCCATTC TCACCCGACA TGATCGCCAG TCACTTTCTG 
CACGCTTTCA TCGTGGTCCA GCCCATTGAG CCGAATACAC CACATACCCG GTACAAGGTC 
AGCGTAACCG CCAGGGATGA TGTTCCCTTC TTTGGTCCCA CTCTGCCGAA TCCCGCAGTT 
TTCCGCAAGG GACAGGAGTT CAAGGAGTTC ATCTTGACCA AGCTGATCAA TGCTGAGAAT 
GCGTGCTACA AAGCTGAGAA GTTTGCAAAG TTGGAACAGC GCACCAGGAC CTCTCTGCTC 
CAGAATTTAT GCGAGGAACT GCGGGAGAAG ACTCGCGACT TTCTGGGAAC CGATCTTTCC 
CAGACGTCGG CAGGAAGTCC TACACCCGAG ACCCCGAAAG CAGAAAGTGG TAGTGGCGGC 
AATGCCGGCT CCCGCTTTAT CGATACGGTG AAGAAGGCTT TAATCATGAG GGTGCGCTCA 
CAGAGCGTGG ACACCGGAAA TGGTCATGGA CCTGGACAAA CGGATAAATT CAGTGTGAAC 
ACGAAGCTGG GCACATTGAA TTCCAAGAAG GAGACCCATC CCGAAATGCA GACACCCAAT 
CTAAATACCT GCAGTCGCAC GGCCTCCAAG TCCTCATCCA AGTCGAAGTC ATCGAACGAG 
TCCACTTCCT CCTCCCCGGA CATTACCTCT CGCCAGGCGA ACAACAACAA TACCAATTCT 
ATAGGTGGAC ACCTCCCCCA AAATGGAACC GGCGGGGGCG TGGTAGCAGC GGCTGTGAGT 
GCCGCCCAAA ACGGAGTGGT CGCAGTGGCC ACCATGTCGG AAACGAGCGA TGATTCCAGT 
CTGAATAGCG TGGATCTCGA TCCAATGATG GCGCACTTGG ACGGCGGAGC CACCTACATT 
GACAGTGATA CTGGGCTAGA AAGCATGAGT TCGGCAGAGG CCACCACCAA GGCGTGCTCC 
CTGTGCTTAG ATGGTGTCCA GTCCACCATA ATGGGTAGTT CCCCCAGCAA CGAGACGATC 
GTGAAGATCG AGAATCTGCG CCAGGAGGTC ACACGTTTGA AGTGCGACAA GCTGGATCTA 
CTCAGACAGA ATGTGACCTG TCAGCGCGAC ATCAAGCGAC TTCGGGAGCG GGAACTTTCC 
CTTCAAGGCG ATCTATCGGC AGCTGGCAGA GAAATCCTAC GACTGCGGGA TCTCCTCAAG 
GAATATATGC CCGATACAGC TTCAGCACCA CTCTCCATAT CGCCCATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RapGAP1 ( NM_001014478.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014478.1 Drosophila melanogaster Rap GTPase activating protein 1 (RapGAP1), transcript variant E, mRNA.

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CloneID	OFa16370
Clone ID Related Accession (Same CDS sequence)	NM_001014478.2
Accession Version	NM_001014478.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rap GTPase activating protein 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001014478.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGGGTAT TGAGATGGCG CGATTCCCCC GGATCGCGGA TATCGGGCTC AATTGGCAAT 
AGCAGCGGCA ACAGCAACAA CAATCACCAC CACAGCAGCA ATAGCAACAG CAGCAGCGGC 
AACAACAACG CTCATCAGCA GCAACACAAC AACAATCACA TCAACAATAA TATGAACAAC 
AACAACAATC GCGAGAACCA CCATCGCGGC AGTAATGCGA GTGAGGAGCG CGTGCCCCAG 
CCACACTCAC CCCACGACAG TCACGCCCAT CCGCACAACG GTGGGCTGCA GCACTCGGGA 
TCGCGGGCAA CACTGCGCCA CTCGATGAAT CACTCGAGCA GCTCCACAGT CTCGGGCTCC 
GCCTGCAGCT CGACGCCCGC CTCCCCGCAA CTGAGTGGGG TGGTCAGCGC CTCTGGATGC 
CAGCTGCTGT CCAACGGACA CCACTACCAA TCTCAGTCGT CGGTGAGCTC CAACTCATCG 
ATCGTTTCGG CCATTCCCGC CTCGCAGAGA TTGCTGGAGG AGGCGCTGGC CAAACCAGCA 
CCGTACCCCA TGATCTTGCT GCCACTGCAC GGTGGTTACT GGATGGATGG CACCGAACAT 
GAGTGCGGCT ACGATGCGAG AGGAAATCCT CTGCTACCAC AGACTACCTG GATGGCAAAG 
TTCGAGACGG ACGATACGGC CAAGTGCTAC AGGAGATTCT ATGCCGCCAG GGAGCACTCG 
AATTTGATTG GACTGGATGA GCAGTTGGGA CCGATTTTGC TTTCTATTAA AACGGAAAAT 
GTAGCCAACC AGGAGCACAT GCGAATCCTG ATGAGATTGA GAACTGGTAC GATGCATGAG 
CTTCTACCGG TATCATGCCT ATTACCACAG CCAAGTCCGG CAAAAATGGC GCATACACTG 
AACGAAAATA TCACAGTCGA GCATTTCATG CCGATTTTGT GCCCCAAGGC CTCGCAACTC 
ATCAGTGTAT ACGACGAGCA TGTTCTCGTT TCGCACTTCA AGTTTGGAGT GCTCTACCAA 
AGATATGGAC AGACCACCGA GGAGGAACTT TTCGGTAACC AGCAGACATC ACCAGCTTTC 
GACGAATTCT TGGATGTCTT GGGTCAAAGG ATACGTCTGA AGGATCACAA GGGCTACAGG 
GGTGGGCTTG ACATTCAGAA TGGTCACACG GGCGACACAG CTGTCTATGA GGTCTTCAAG 
GAGCGAGAGA TCATGTTCCA TGTATCCACA CTATTGCCGC ACACCGAAGG CGATCCACAG 
CAGCTCCAGA GGAAGCGACA CATTGGCAAT GACATTGTGG CCATCGTTTT TCAGGAGACT 
AACACTCCAT TCTCACCCGA CATGATCGCC AGTCACTTTC TGCACGCTTT CATCGTGGTC 
CAGCCCATTG AGCCGAATAC ACCACATACC CGGTACAAGG TCAGCGTAAC CGCCAGGGAT 
GATGTTCCCT TCTTTGGTCC CACTCTGCCG AATCCCGCAG TTTTCCGCAA GGGACAGGAG 
TTCAAGGAGT TCATCTTGAC CAAGCTGATC AATGCTGAGA ATGCGTGCTA CAAAGCTGAG 
AAGTTTGCAA AGTTGGAACA GCGCACCAGG ACCTCTCTGC TCCAGAATTT ATGCGAGGAA 
CTGCGGGAGA AGACTCGCGA CTTTCTGGGA ACCGATCTTT CCCAGACGTC GGCAGGAAGT 
CCTACACCCG AGACCCCGAA AGCAGAAAGT GGTAGTGGCG GCAATGCCGG CTCCCGCTTT 
ATCGATACGG TGAAGAAGGC TTTAATCATG AGGGTGCGCT CACAGAGCGT GGACACCGGA 
AATGGTCATG GACCTGGACA AACGGATAAA TTCAGTGTGA ACACGAAGCT GGGCACATTG 
AATTCCAAGA AGGAGACCCA TCCCGAAATG CAGACACCCA ATCTAAATAC CTGCAGTCGC 
ACGGCCTCCA AGTCCTCATC CAAGTCGAAG TCATCGAACG AGTCCACTTC CTCCTCCCCG 
GACATTACCT CTCGCCAGGC GAACAACAAC AATACCAATT CTATAGGTGG ACACCTCCCC 
CAAAATGGAA CCGGCGGGGG CGTGGTAGCA GCGGCTGTGA GTGCCGCCCA AAACGGAGTG 
GTCGCAGTGG CCACCATGTC GGAAACGAGC GATGATTCCA GTCTGAATAG CGTGGATCTC 
GATCCAATGA TGGCGCACTT GGACGGCGGA GCCACCTACA TTGACAGTGA TACTGGGCTA 
GAAAGCATGA GTTCGGCAGA GGCCACCACC AAGGCGTGCT CCCTGTGCTT AGATGGTGTC 
CAGTCCACCA TAATGGGTAG TTCCCCCAGC AACGAGACGA TCGTGAAGAT CGAGAATCTG 
CGCCAGGAGG TCACACGTTT GAAGTGCGAC AAGCTGGATC TACTCAGACA GAATGTGACC 
TGTCAGCGCG ACATCAAGCG ACTTCGGGAG CGGGAACTTT CCCTTCAAGG CGATCTATCG 
GCAGCTGGCA GAGAAATCCT ACGACTGCGG GATCTCCTCA AGGAATATAT GCCCGATACA 
GCTTCAGCAC CACTCTCCAT ATCGCCCATC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014478.1
CDS	1179..3731
Translation	MGVLRWRDSPGSRISGSIGNSSGNSNNNHHHSSNSNSSSGNNNAHQQQHNNNHINNNMNNNNNRENHHRGSNASEERVPQPHSPHDSHAHPHNGGLQHSGSRATLRHSMNHSSSSTVSGSACSSTPASPQLSGVVSASGCQLLSNGHHYQSQSSVSSNSSIVSAIPASQRLLEEALAKPAPYPMILLPLHGGYWMDGTEHECGYDARGNPLLPQTTWMAKFETDDTAKCYRRFYAAREHSNLIGLDEQLGPILLSIKTENVANQEHMRILMRLRTGTMHELLPVSCLLPQPSPAKMAHTLNENITVEHFMPILCPKASQLISVYDEHVLVSHFKFGVLYQRYGQTTEEELFGNQQTSPAFDEFLDVLGQRIRLKDHKGYRGGLDIQNGHTGDTAVYEVFKEREIMFHVSTLLPHTEGDPQQLQRKRHIGNDIVAIVFQETNTPFSPDMIASHFLHAFIVVQPIEPNTPHTRYKVSVTARDDVPFFGPTLPNPAVFRKGQEFKEFILTKLINAENACYKAEKFAKLEQRTRTSLLQNLCEELREKTRDFLGTDLSQTSAGSPTPETPKAESGSGGNAGSRFIDTVKKALIMRVRSQSVDTGNGHGPGQTDKFSVNTKLGTLNSKKETHPEMQTPNLNTCSRTASKSSSKSKSSNESTSSSPDITSRQANNNNTNSIGGHLPQNGTGGGVVAAAVSAAQNGVVAVATMSETSDDSSLNSVDLDPMMAHLDGGATYIDSDTGLESMSSAEATTKACSLCLDGVQSTIMGSSPSNETIVKIENLRQEVTRLKCDKLDLLRQNVTCQRDIKRLRERELSLQGDLSAAGREILRLRDLLKEYMPDTASAPLSISPI

Target ORF information:

RefSeq Version	NM_001014478.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rap GTPase activating protein 1 (RapGAP1), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014478.2

ORF Insert Sequence:

ATGGGGGTAT TGAGATGGCG CGATTCCCCC GGATCGCGGA TATCGGGCTC AATTGGCAAT 
AGCAGCGGCA ACAGCAACAA CAATCACCAC CACAGCAGCA ATAGCAACAG CAGCAGCGGC 
AACAACAACG CTCATCAGCA GCAACACAAC AACAATCACA TCAACAATAA TATGAACAAC 
AACAACAATC GCGAGAACCA CCATCGCGGC AGTAATGCGA GTGAGGAGCG CGTGCCCCAG 
CCACACTCAC CCCACGACAG TCACGCCCAT CCGCACAACG GTGGGCTGCA GCACTCGGGA 
TCGCGGGCAA CACTGCGCCA CTCGATGAAT CACTCGAGCA GCTCCACAGT CTCGGGCTCC 
GCCTGCAGCT CGACGCCCGC CTCCCCGCAA CTGAGTGGGG TGGTCAGCGC CTCTGGATGC 
CAGCTGCTGT CCAACGGACA CCACTACCAA TCTCAGTCGT CGGTGAGCTC CAACTCATCG 
ATCGTTTCGG CCATTCCCGC CTCGCAGAGA TTGCTGGAGG AGGCGCTGGC CAAACCAGCA 
CCGTACCCCA TGATCTTGCT GCCACTGCAC GGTGGTTACT GGATGGATGG CACCGAACAT 
GAGTGCGGCT ACGATGCGAG AGGAAATCCT CTGCTACCAC AGACTACCTG GATGGCAAAG 
TTCGAGACGG ACGATACGGC CAAGTGCTAC AGGAGATTCT ATGCCGCCAG GGAGCACTCG 
AATTTGATTG GACTGGATGA GCAGTTGGGA CCGATTTTGC TTTCTATTAA AACGGAAAAT 
GTAGCCAACC AGGAGCACAT GCGAATCCTG ATGAGATTGA GAACTGGTAC GATGCATGAG 
CTTCTACCGG TATCATGCCT ATTACCACAG CCAAGTCCGG CAAAAATGGC GCATACACTG 
AACGAAAATA TCACAGTCGA GCATTTCATG CCGATTTTGT GCCCCAAGGC CTCGCAACTC 
ATCAGTGTAT ACGACGAGCA TGTTCTCGTT TCGCACTTCA AGTTTGGAGT GCTCTACCAA 
AGATATGGAC AGACCACCGA GGAGGAACTT TTCGGTAACC AGCAGACATC ACCAGCTTTC 
GACGAATTCT TGGATGTCTT GGGTCAAAGG ATACGTCTGA AGGATCACAA GGGCTACAGG 
GGTGGGCTTG ACATTCAGAA TGGTCACACG GGCGACACAG CTGTCTATGA GGTCTTCAAG 
GAGCGAGAGA TCATGTTCCA TGTATCCACA CTATTGCCGC ACACCGAAGG CGATCCACAG 
CAGCTCCAGA GGAAGCGACA CATTGGCAAT GACATTGTGG CCATCGTTTT TCAGGAGACT 
AACACTCCAT TCTCACCCGA CATGATCGCC AGTCACTTTC TGCACGCTTT CATCGTGGTC 
CAGCCCATTG AGCCGAATAC ACCACATACC CGGTACAAGG TCAGCGTAAC CGCCAGGGAT 
GATGTTCCCT TCTTTGGTCC CACTCTGCCG AATCCCGCAG TTTTCCGCAA GGGACAGGAG 
TTCAAGGAGT TCATCTTGAC CAAGCTGATC AATGCTGAGA ATGCGTGCTA CAAAGCTGAG 
AAGTTTGCAA AGTTGGAACA GCGCACCAGG ACCTCTCTGC TCCAGAATTT ATGCGAGGAA 
CTGCGGGAGA AGACTCGCGA CTTTCTGGGA ACCGATCTTT CCCAGACGTC GGCAGGAAGT 
CCTACACCCG AGACCCCGAA AGCAGAAAGT GGTAGTGGCG GCAATGCCGG CTCCCGCTTT 
ATCGATACGG TGAAGAAGGC TTTAATCATG AGGGTGCGCT CACAGAGCGT GGACACCGGA 
AATGGTCATG GACCTGGACA AACGGATAAA TTCAGTGTGA ACACGAAGCT GGGCACATTG 
AATTCCAAGA AGGAGACCCA TCCCGAAATG CAGACACCCA ATCTAAATAC CTGCAGTCGC 
ACGGCCTCCA AGTCCTCATC CAAGTCGAAG TCATCGAACG AGTCCACTTC CTCCTCCCCG 
GACATTACCT CTCGCCAGGC GAACAACAAC AATACCAATT CTATAGGTGG ACACCTCCCC 
CAAAATGGAA CCGGCGGGGG CGTGGTAGCA GCGGCTGTGA GTGCCGCCCA AAACGGAGTG 
GTCGCAGTGG CCACCATGTC GGAAACGAGC GATGATTCCA GTCTGAATAG CGTGGATCTC 
GATCCAATGA TGGCGCACTT GGACGGCGGA GCCACCTACA TTGACAGTGA TACTGGGCTA 
GAAAGCATGA GTTCGGCAGA GGCCACCACC AAGGCGTGCT CCCTGTGCTT AGATGGTGTC 
CAGTCCACCA TAATGGGTAG TTCCCCCAGC AACGAGACGA TCGTGAAGAT CGAGAATCTG 
CGCCAGGAGG TCACACGTTT GAAGTGCGAC AAGCTGGATC TACTCAGACA GAATGTGACC 
TGTCAGCGCG ACATCAAGCG ACTTCGGGAG CGGGAACTTT CCCTTCAAGG CGATCTATCG 
GCAGCTGGCA GAGAAATCCT ACGACTGCGG GATCTCCTCA AGGAATATAT GCCCGATACA 
GCTTCAGCAC CACTCTCCAT ATCGCCCATC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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RapGAP1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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