cup cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	cup
Entrez Gene ID	33934
Full Name	CG11181 gene product from transcript CG11181-RC
Synonyms	CG11181,CUP,Cup,DmCup,Dmel\CG11181,fs(1)cup,fs(2)cup,i239
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 26F6-26F7\|2-23 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_078769.3	NP_523493.3	cup, isoform B
NM_001298758.1	NP_001285687.1	cup, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

loss of cup disrupts the localization of both the oskar mRNA and its associated proteins to the posterior pole of the developing oocyte.
Title: Cup regulates oskar mRNA stability during oogenesis.

cup mutants have increased numbers of satellite boutons and EPSP. cup genetically interacts with EndoA and Dap160.
Title: The translational regulator Cup controls NMJ presynaptic terminal morphology.

The structure provides insight into the mechanism by which Cup disrupts eIF4E-eIF4G interaction and has broader implications for understanding the role of 4E-BPs in translational regulation.
Title: Crystal structure of a minimal eIF4E-Cup complex reveals a general mechanism of eIF4E regulation in translational repression.

Cup promotes the accumulation of the germ plasm determinants at the posterior pole of the oocyte.
Title: The translational repressor Cup is required for germ cell development in Drosophila.

Cup blocks the precocious activation of the orb autoregulatory loop
Title: Cup blocks the precocious activation of the orb autoregulatory loop.

The following cup gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cup cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16313	NM_001298758.1 Latest version!	Drosophila melanogaster cup, transcript variant C (cup), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa16313	NM_078769.3 Latest version!	Drosophila melanogaster cup, transcript variant B (cup), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cup ( NM_078769.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523493.3 Drosophila melanogaster cup, transcript variant B (cup), mRNA.

Price & Availability↑

CloneID	OFa16313
Clone ID Related Accession (Same CDS sequence)	NM_078769.3 , NM_001298758.1
Accession Version	NM_078769.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cup, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_078769.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAATGG CCGAAGCTGA GCAAGAAAAT GGCGCTGGCG CCCTGAAAAT TGCCACCAAT 
GCGGGTGCAA CCGATCGTCC AGCGCATCAG CAGCTTCCGC TGCCAGTCGA AGATCAGCAG 
GATGAGGTGC TGACCCCCGC TGAGAAGGGC AAGTTCGAGT ACCCGCCCCC TCCACCGCCA 
CCAACTCCAG TCCAGGCTCC GCTAGCCACC AAGGCCACCG CTCTGAATGC CAGTCAAGAG 
CATGACGACG ACGAGGCCAA CTCCGAGAAG TGGGAGGATC CGTGCGCTCC GCCCCCGCCT 
CCTCCGCTGC CGACTAGCGC TTTCCTGGCC ACCGGACTGG GCTACCTCAA GCTGCCCGCC 
TTCAAGCTGA AGGACGCACT GGAGAAGGCC ATCACCAAGT TGGAGGCCAA CAAGCGGACG 
CTAAAGGCCA GCCCTGAGTC TTCTCGTTCC ATCAAAAACA AGAATGTGGT GGCCCTGGAG 
ATGTTACCCA GGCGCTCAAA CCCAGAAACG ATCGGCGATG GATCCATGTT GGCATCCACA 
TCCACGGCTG TTATGCTGCA GACCAAAAAG CCCGCCGTTA TTGTGGAGAT GGAGAGGCGT 
TGTAAGATCA TCAATTTACT GGCCAAACAG AATCAAATCC TCGAATCCAT TTCGGGGGAG 
GCCATTCCGA TGCATGGACC CTCGAAGCAT CTGCACGAGG ACGAGGGTCT GACCCTGCAA 
GTTCTCTCGG CCCGAGCATC CACACCGTAC ACACAGCCCT CCAGCATGTT GTCCTGCACG 
GCCGTTAGTT GTGACCTGGA GCACGATTCG CCGCGGAAGC AAGTGGCCAG CAAGGAGGCC 
GTGCCGGAGC AGCAGTCTAG TCAGGTGCAG CAAAAGCGTC CACCCAGCAC AGGCATTCAC 
AAGCCAGGAA GTCTGCGAGC ACCCAAGGCT GTTCGTCCTA CTACTGCTCC GGTGGTCAGC 
AGCAAGCCGG TCAAGAGCTA TACCCGCTCC CGCCTGATGG ACATTCGCAA CGGGATGTTC 
AATGCCCTGA TGCACAGGTC CAAGGAGAGC TTTGTGATGC CTCGCATCGC CACCTGCGAC 
GACATTGAGT TGGAGGGAAG GCTTCGCCGC ATGAATATTT GGCGCACCTC GGACGGCACA 
AGATTCCGGA CCCGCTCCAC CACAGCTAAC TTAAACATGA ACAACAACAA CAACAATGAG 
TGCATGCCTG CCTTCTTCAA AAACAAAAAT AAGCCGAACC TAATCAGTGA CGAGTCCATC 
ATCCAGAGCC AACCGCCCCA GCCGCAAACG GAGTTCCAAG ATCCGGCCAT TGTTAACCAA 
AGACGCATTG GCAGCGGTCG CTTAAACCAT TCCAAGTGGG GTTACAACGA CGAAGACTAC 
CACTCGTACC ACAATGGCAA GTCGCAGCAT ATGGAGGAGG TCAACTCGAA GAACAGCAAA 
AACATGACGG TGCTGCAGTT CTTCGACAAC GGCGAGATCA GCAGTCAGCC TCAAAGACGG 
CCGAACACTC CTGTGATGGG GATGTCTATC AATCGATCCG AGAACGACAC CCTGCACTCG 
AACGAGTCCA GTGAAGATTT GAGTCGCGCC AACGAGAACT ATGTAAAGCG CGTAATGTCT 
GGATTCCTGG TTGTATCGAA GCCCAAGTCA CGAGATGTCG AGGATAGACA TCACCGGCGG 
TACAGGAACC AGAACGAGGA GCCTGAGTGG TTTAGCTGTG GACCCACGTC TAGACTGGAC 
ACTATAGAGT TGTGTGGGTT CGATGAGGAC GAGGAGAAAA TGCTAAAGGA GGGCAACAAA 
AACCACGGAT TAGGCGAAAC AGAGCGAGAA ACGTCAAAAC AAAAGATGGA CCATAAGTAC 
AAATGGACGC ATGCGGAGCC GATGGGTCGA AGCAAATACA TGCCCAAACA CGACACTAAC 
AATAATCATA ACGTGGAGAA CATGAATAAT GTTATGGCTA CCGAGCATCA GCAACAAAAG 
GAAGAGAAAC GCCCAGGCAG TGGCCGTTCG TTTCAGTTTG ATAAGTTCAA TCAAAGCCAA 
CAGAATTACG AGAGTAGCAG TTATGTTAAT CACCAACAGC CGCCGCAAAC GCAGCCTCAG 
CAAATGCAGC AGCAGTCGAA CACAAACACG AACAACTCTA AATTCATGTC ATTCTTTGCC 
AATGAGGGGA ACTCCTCGTC TTCTTCGCTG AACGAGTTTT TCAAGCAGGC CATTAACCAG 
GGTCATGGCA ACAATCCGGA GCAGCCCAAA TCGTTGGGCC ACATCGGACA GATGCCTTCG 
GTGGATCAGC TGGAGGCCAA ATGGCGTCGA AACTCACTGA ACAATGTTGG CGAGACTGCC 
AACAAGCAGA CTGATAACTT CCAGAAGCTA ATTGGGTCTC TTTCTTCGGC TAAGCCACAG 
TCACAGGCGG TTGGCTATGA TGCCATCTCC AACTTTATAA TGCAGCAACA GCAGTACCAG 
CAGCAGCAAC AGAAGCAGCA CCTCATCATA CAGCAGCAGC AGCAACATAC CGCCTTTTTG 
GCGAGCCTGC AGCTGAAGGC GATCCTCGGC CGGGCCGACA CCCAATTGCT ACTGCTCCGT 
CTGACTAAAG GGGAAATATC CAAGCACGGA TTGCTGGTGC AGTTGGCCAA CCCACGCTTG 
ACGGACATGG ATCGCGAGGC CATCACTGCT GTATTGCAGT TCACCAACAC CCAGCAGCAG 
CAGCAGCAGC ACAAGCAGCA GCTGGACATG CTCTCAAGCA CGGTCATCGC CAGCCAGTTG 
CAGAATCTCC ACAACTTGGC CATTGTCCAG CAGACTCTTG CAGCCAGGCA GCAGCCGCAG 
CATAATCCAC AGACGCAGGC GCCGCACCAA CTGTCGCAGG AGGACTTGCA AGCGCATGCC 
AATGTGATAA TGCGAAACGC CGTAATGAAG CGAAAAATTG AGGAGCAGAC CTCGAAGCTT 
ATCAATGGCG GTGCCAAGCA TCAGGCACAG CAGCAATATC TAAACCGTGG CCAGCAACGC 
CAGGCTCGAC CAGATGCCAA CTCGAATGCC TTGCTCCATG CCTTGATCTC TGGCGGTGGC 
AATAACCACG CCTCTGGTTA TCCTATGAAT GGACAGCCGC AGAAGCATCA TTCCAATTTG 
AGATTCGGTG ATAACCAAAA CTTCCAATCT TTTGAGTCCA ACCAGCCGCA CTTCGCCACG 
CAGTACAAGC AGCAATACCA ACAGTCTCAG CAGCAACATC CCCATCAGCA GCCCCAACAG 
CTGAATTCGC TTCATCAGAA CAACGCTGGA GCTGTCAATA GCTTCAATAA GGCCCAAATG 
CAAGCTCAAT CAGCTATTTC CATGCTGCCC AACAGCGGGG ATGAGTTTCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523493.3
CDS	183..3536
Translation	MQMAEAEQENGAGALKIATNAGATDRPAHQQLPLPVEDQQDEVLTPAEKGKFEYPPPPPPPTPVQAPLATKATALNASQEHDDDEANSEKWEDPCAPPPPPPLPTSAFLATGLGYLKLPAFKLKDALEKAITKLEANKRTLKASPESSRSIKNKNVVALEMLPRRSNPETIGDGSMLASTSTAVMLQTKKPAVIVEMERRCKIINLLAKQNQILESISGEAIPMHGPSKHLHEDEGLTLQVLSARASTPYTQPSSMLSCTAVSCDLEHDSPRKQVASKEAVPEQQSSQVQQKRPPSTGIHKPGSLRAPKAVRPTTAPVVSSKPVKSYTRSRLMDIRNGMFNALMHRSKESFVMPRIATCDDIELEGRLRRMNIWRTSDGTRFRTRSTTANLNMNNNNNNECMPAFFKNKNKPNLISDESIIQSQPPQPQTEFQDPAIVNQRRIGSGRLNHSKWGYNDEDYHSYHNGKSQHMEEVNSKNSKNMTVLQFFDNGEISSQPQRRPNTPVMGMSINRSENDTLHSNESSEDLSRANENYVKRVMSGFLVVSKPKSRDVEDRHHRRYRNQNEEPEWFSCGPTSRLDTIELCGFDEDEEKMLKEGNKNHGLGETERETSKQKMDHKYKWTHAEPMGRSKYMPKHDTNNNHNVENMNNVMATEHQQQKEEKRPGSGRSFQFDKFNQSQQNYESSSYVNHQQPPQTQPQQMQQQSNTNTNNSKFMSFFANEGNSSSSSLNEFFKQAINQGHGNNPEQPKSLGHIGQMPSVDQLEAKWRRNSLNNVGETANKQTDNFQKLIGSLSSAKPQSQAVGYDAISNFIMQQQQYQQQQQKQHLIIQQQQQHTAFLASLQLKAILGRADTQLLLLRLTKGEISKHGLLVQLANPRLTDMDREAITAVLQFTNTQQQQQQHKQQLDMLSSTVIASQLQNLHNLAIVQQTLAARQQPQHNPQTQAPHQLSQEDLQAHANVIMRNAVMKRKIEEQTSKLINGGAKHQAQQQYLNRGQQRQARPDANSNALLHALISGGGNNHASGYPMNGQPQKHHSNLRFGDNQNFQSFESNQPHFATQYKQQYQQSQQQHPHQQPQQLNSLHQNNAGAVNSFNKAQMQAQSAISMLPNSGDEFH

Target ORF information:

RefSeq Version	NM_078769.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cup, transcript variant B (cup), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078769.3

ORF Insert Sequence:

ATGCAAATGG CCGAAGCTGA GCAAGAAAAT GGCGCTGGCG CCCTGAAAAT TGCCACCAAT 
GCGGGTGCAA CCGATCGTCC AGCGCATCAG CAGCTTCCGC TGCCAGTCGA AGATCAGCAG 
GATGAGGTGC TGACCCCCGC TGAGAAGGGC AAGTTCGAGT ACCCGCCCCC TCCACCGCCA 
CCAACTCCAG TCCAGGCTCC GCTAGCCACC AAGGCCACCG CTCTGAATGC CAGTCAAGAG 
CATGACGACG ACGAGGCCAA CTCCGAGAAG TGGGAGGATC CGTGCGCTCC GCCCCCGCCT 
CCTCCGCTGC CGACTAGCGC TTTCCTGGCC ACCGGACTGG GCTACCTCAA GCTGCCCGCC 
TTCAAGCTGA AGGACGCACT GGAGAAGGCC ATCACCAAGT TGGAGGCCAA CAAGCGGACG 
CTAAAGGCCA GCCCTGAGTC TTCTCGTTCC ATCAAAAACA AGAATGTGGT GGCCCTGGAG 
ATGTTACCCA GGCGCTCAAA CCCAGAAACG ATCGGCGATG GATCCATGTT GGCATCCACA 
TCCACGGCTG TTATGCTGCA GACCAAAAAG CCCGCCGTTA TTGTGGAGAT GGAGAGGCGT 
TGTAAGATCA TCAATTTACT GGCCAAACAG AATCAAATCC TCGAATCCAT TTCGGGGGAG 
GCCATTCCGA TGCATGGACC CTCGAAGCAT CTGCACGAGG ACGAGGGTCT GACCCTGCAA 
GTTCTCTCGG CCCGAGCATC CACACCGTAC ACACAGCCCT CCAGCATGTT GTCCTGCACG 
GCCGTTAGTT GTGACCTGGA GCACGATTCG CCGCGGAAGC AAGTGGCCAG CAAGGAGGCC 
GTGCCGGAGC AGCAGTCTAG TCAGGTGCAG CAAAAGCGTC CACCCAGCAC AGGCATTCAC 
AAGCCAGGAA GTCTGCGAGC ACCCAAGGCT GTTCGTCCTA CTACTGCTCC GGTGGTCAGC 
AGCAAGCCGG TCAAGAGCTA TACCCGCTCC CGCCTGATGG ACATTCGCAA CGGGATGTTC 
AATGCCCTGA TGCACAGGTC CAAGGAGAGC TTTGTGATGC CTCGCATCGC CACCTGCGAC 
GACATTGAGT TGGAGGGAAG GCTTCGCCGC ATGAATATTT GGCGCACCTC GGACGGCACA 
AGATTCCGGA CCCGCTCCAC CACAGCTAAC TTAAACATGA ACAACAACAA CAACAATGAG 
TGCATGCCTG CCTTCTTCAA AAACAAAAAT AAGCCGAACC TAATCAGTGA CGAGTCCATC 
ATCCAGAGCC AACCGCCCCA GCCGCAAACG GAGTTCCAAG ATCCGGCCAT TGTTAACCAA 
AGACGCATTG GCAGCGGTCG CTTAAACCAT TCCAAGTGGG GTTACAACGA CGAAGACTAC 
CACTCGTACC ACAATGGCAA GTCGCAGCAT ATGGAGGAGG TCAACTCGAA GAACAGCAAA 
AACATGACGG TGCTGCAGTT CTTCGACAAC GGCGAGATCA GCAGTCAGCC TCAAAGACGG 
CCGAACACTC CTGTGATGGG GATGTCTATC AATCGATCCG AGAACGACAC CCTGCACTCG 
AACGAGTCCA GTGAAGATTT GAGTCGCGCC AACGAGAACT ATGTAAAGCG CGTAATGTCT 
GGATTCCTGG TTGTATCGAA GCCCAAGTCA CGAGATGTCG AGGATAGACA TCACCGGCGG 
TACAGGAACC AGAACGAGGA GCCTGAGTGG TTTAGCTGTG GACCCACGTC TAGACTGGAC 
ACTATAGAGT TGTGTGGGTT CGATGAGGAC GAGGAGAAAA TGCTAAAGGA GGGCAACAAA 
AACCACGGAT TAGGCGAAAC AGAGCGAGAA ACGTCAAAAC AAAAGATGGA CCATAAGTAC 
AAATGGACGC ATGCGGAGCC GATGGGTCGA AGCAAATACA TGCCCAAACA CGACACTAAC 
AATAATCATA ACGTGGAGAA CATGAATAAT GTTATGGCTA CCGAGCATCA GCAACAAAAG 
GAAGAGAAAC GCCCAGGCAG TGGCCGTTCG TTTCAGTTTG ATAAGTTCAA TCAAAGCCAA 
CAGAATTACG AGAGTAGCAG TTATGTTAAT CACCAACAGC CGCCGCAAAC GCAGCCTCAG 
CAAATGCAGC AGCAGTCGAA CACAAACACG AACAACTCTA AATTCATGTC ATTCTTTGCC 
AATGAGGGGA ACTCCTCGTC TTCTTCGCTG AACGAGTTTT TCAAGCAGGC CATTAACCAG 
GGTCATGGCA ACAATCCGGA GCAGCCCAAA TCGTTGGGCC ACATCGGACA GATGCCTTCG 
GTGGATCAGC TGGAGGCCAA ATGGCGTCGA AACTCACTGA ACAATGTTGG CGAGACTGCC 
AACAAGCAGA CTGATAACTT CCAGAAGCTA ATTGGGTCTC TTTCTTCGGC TAAGCCACAG 
TCACAGGCGG TTGGCTATGA TGCCATCTCC AACTTTATAA TGCAGCAACA GCAGTACCAG 
CAGCAGCAAC AGAAGCAGCA CCTCATCATA CAGCAGCAGC AGCAACATAC CGCCTTTTTG 
GCGAGCCTGC AGCTGAAGGC GATCCTCGGC CGGGCCGACA CCCAATTGCT ACTGCTCCGT 
CTGACTAAAG GGGAAATATC CAAGCACGGA TTGCTGGTGC AGTTGGCCAA CCCACGCTTG 
ACGGACATGG ATCGCGAGGC CATCACTGCT GTATTGCAGT TCACCAACAC CCAGCAGCAG 
CAGCAGCAGC ACAAGCAGCA GCTGGACATG CTCTCAAGCA CGGTCATCGC CAGCCAGTTG 
CAGAATCTCC ACAACTTGGC CATTGTCCAG CAGACTCTTG CAGCCAGGCA GCAGCCGCAG 
CATAATCCAC AGACGCAGGC GCCGCACCAA CTGTCGCAGG AGGACTTGCA AGCGCATGCC 
AATGTGATAA TGCGAAACGC CGTAATGAAG CGAAAAATTG AGGAGCAGAC CTCGAAGCTT 
ATCAATGGCG GTGCCAAGCA TCAGGCACAG CAGCAATATC TAAACCGTGG CCAGCAACGC 
CAGGCTCGAC CAGATGCCAA CTCGAATGCC TTGCTCCATG CCTTGATCTC TGGCGGTGGC 
AATAACCACG CCTCTGGTTA TCCTATGAAT GGACAGCCGC AGAAGCATCA TTCCAATTTG 
AGATTCGGTG ATAACCAAAA CTTCCAATCT TTTGAGTCCA ACCAGCCGCA CTTCGCCACG 
CAGTACAAGC AGCAATACCA ACAGTCTCAG CAGCAACATC CCCATCAGCA GCCCCAACAG 
CTGAATTCGC TTCATCAGAA CAACGCTGGA GCTGTCAATA GCTTCAATAA GGCCCAAATG 
CAAGCTCAAT CAGCTATTTC CATGCTGCCC AACAGCGGGG ATGAGTTTCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cup ( NM_001298758.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285687.1 Drosophila melanogaster cup, transcript variant C (cup), mRNA.

Price & Availability↑

CloneID	OFa16313
Clone ID Related Accession (Same CDS sequence)	NM_078769.3 , NM_001298758.1
Accession Version	NM_001298758.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cup, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAATGG CCGAAGCTGA GCAAGAAAAT GGCGCTGGCG CCCTGAAAAT TGCCACCAAT 
GCGGGTGCAA CCGATCGTCC AGCGCATCAG CAGCTTCCGC TGCCAGTCGA AGATCAGCAG 
GATGAGGTGC TGACCCCCGC TGAGAAGGGC AAGTTCGAGT ACCCGCCCCC TCCACCGCCA 
CCAACTCCAG TCCAGGCTCC GCTAGCCACC AAGGCCACCG CTCTGAATGC CAGTCAAGAG 
CATGACGACG ACGAGGCCAA CTCCGAGAAG TGGGAGGATC CGTGCGCTCC GCCCCCGCCT 
CCTCCGCTGC CGACTAGCGC TTTCCTGGCC ACCGGACTGG GCTACCTCAA GCTGCCCGCC 
TTCAAGCTGA AGGACGCACT GGAGAAGGCC ATCACCAAGT TGGAGGCCAA CAAGCGGACG 
CTAAAGGCCA GCCCTGAGTC TTCTCGTTCC ATCAAAAACA AGAATGTGGT GGCCCTGGAG 
ATGTTACCCA GGCGCTCAAA CCCAGAAACG ATCGGCGATG GATCCATGTT GGCATCCACA 
TCCACGGCTG TTATGCTGCA GACCAAAAAG CCCGCCGTTA TTGTGGAGAT GGAGAGGCGT 
TGTAAGATCA TCAATTTACT GGCCAAACAG AATCAAATCC TCGAATCCAT TTCGGGGGAG 
GCCATTCCGA TGCATGGACC CTCGAAGCAT CTGCACGAGG ACGAGGGTCT GACCCTGCAA 
GTTCTCTCGG CCCGAGCATC CACACCGTAC ACACAGCCCT CCAGCATGTT GTCCTGCACG 
GCCGTTAGTT GTGACCTGGA GCACGATTCG CCGCGGAAGC AAGTGGCCAG CAAGGAGGCC 
GTGCCGGAGC AGCAGTCTAG TCAGGTGCAG CAAAAGCGTC CACCCAGCAC AGGCATTCAC 
AAGCCAGGAA GTCTGCGAGC ACCCAAGGCT GTTCGTCCTA CTACTGCTCC GGTGGTCAGC 
AGCAAGCCGG TCAAGAGCTA TACCCGCTCC CGCCTGATGG ACATTCGCAA CGGGATGTTC 
AATGCCCTGA TGCACAGGTC CAAGGAGAGC TTTGTGATGC CTCGCATCGC CACCTGCGAC 
GACATTGAGT TGGAGGGAAG GCTTCGCCGC ATGAATATTT GGCGCACCTC GGACGGCACA 
AGATTCCGGA CCCGCTCCAC CACAGCTAAC TTAAACATGA ACAACAACAA CAACAATGAG 
TGCATGCCTG CCTTCTTCAA AAACAAAAAT AAGCCGAACC TAATCAGTGA CGAGTCCATC 
ATCCAGAGCC AACCGCCCCA GCCGCAAACG GAGTTCCAAG ATCCGGCCAT TGTTAACCAA 
AGACGCATTG GCAGCGGTCG CTTAAACCAT TCCAAGTGGG GTTACAACGA CGAAGACTAC 
CACTCGTACC ACAATGGCAA GTCGCAGCAT ATGGAGGAGG TCAACTCGAA GAACAGCAAA 
AACATGACGG TGCTGCAGTT CTTCGACAAC GGCGAGATCA GCAGTCAGCC TCAAAGACGG 
CCGAACACTC CTGTGATGGG GATGTCTATC AATCGATCCG AGAACGACAC CCTGCACTCG 
AACGAGTCCA GTGAAGATTT GAGTCGCGCC AACGAGAACT ATGTAAAGCG CGTAATGTCT 
GGATTCCTGG TTGTATCGAA GCCCAAGTCA CGAGATGTCG AGGATAGACA TCACCGGCGG 
TACAGGAACC AGAACGAGGA GCCTGAGTGG TTTAGCTGTG GACCCACGTC TAGACTGGAC 
ACTATAGAGT TGTGTGGGTT CGATGAGGAC GAGGAGAAAA TGCTAAAGGA GGGCAACAAA 
AACCACGGAT TAGGCGAAAC AGAGCGAGAA ACGTCAAAAC AAAAGATGGA CCATAAGTAC 
AAATGGACGC ATGCGGAGCC GATGGGTCGA AGCAAATACA TGCCCAAACA CGACACTAAC 
AATAATCATA ACGTGGAGAA CATGAATAAT GTTATGGCTA CCGAGCATCA GCAACAAAAG 
GAAGAGAAAC GCCCAGGCAG TGGCCGTTCG TTTCAGTTTG ATAAGTTCAA TCAAAGCCAA 
CAGAATTACG AGAGTAGCAG TTATGTTAAT CACCAACAGC CGCCGCAAAC GCAGCCTCAG 
CAAATGCAGC AGCAGTCGAA CACAAACACG AACAACTCTA AATTCATGTC ATTCTTTGCC 
AATGAGGGGA ACTCCTCGTC TTCTTCGCTG AACGAGTTTT TCAAGCAGGC CATTAACCAG 
GGTCATGGCA ACAATCCGGA GCAGCCCAAA TCGTTGGGCC ACATCGGACA GATGCCTTCG 
GTGGATCAGC TGGAGGCCAA ATGGCGTCGA AACTCACTGA ACAATGTTGG CGAGACTGCC 
AACAAGCAGA CTGATAACTT CCAGAAGCTA ATTGGGTCTC TTTCTTCGGC TAAGCCACAG 
TCACAGGCGG TTGGCTATGA TGCCATCTCC AACTTTATAA TGCAGCAACA GCAGTACCAG 
CAGCAGCAAC AGAAGCAGCA CCTCATCATA CAGCAGCAGC AGCAACATAC CGCCTTTTTG 
GCGAGCCTGC AGCTGAAGGC GATCCTCGGC CGGGCCGACA CCCAATTGCT ACTGCTCCGT 
CTGACTAAAG GGGAAATATC CAAGCACGGA TTGCTGGTGC AGTTGGCCAA CCCACGCTTG 
ACGGACATGG ATCGCGAGGC CATCACTGCT GTATTGCAGT TCACCAACAC CCAGCAGCAG 
CAGCAGCAGC ACAAGCAGCA GCTGGACATG CTCTCAAGCA CGGTCATCGC CAGCCAGTTG 
CAGAATCTCC ACAACTTGGC CATTGTCCAG CAGACTCTTG CAGCCAGGCA GCAGCCGCAG 
CATAATCCAC AGACGCAGGC GCCGCACCAA CTGTCGCAGG AGGACTTGCA AGCGCATGCC 
AATGTGATAA TGCGAAACGC CGTAATGAAG CGAAAAATTG AGGAGCAGAC CTCGAAGCTT 
ATCAATGGCG GTGCCAAGCA TCAGGCACAG CAGCAATATC TAAACCGTGG CCAGCAACGC 
CAGGCTCGAC CAGATGCCAA CTCGAATGCC TTGCTCCATG CCTTGATCTC TGGCGGTGGC 
AATAACCACG CCTCTGGTTA TCCTATGAAT GGACAGCCGC AGAAGCATCA TTCCAATTTG 
AGATTCGGTG ATAACCAAAA CTTCCAATCT TTTGAGTCCA ACCAGCCGCA CTTCGCCACG 
CAGTACAAGC AGCAATACCA ACAGTCTCAG CAGCAACATC CCCATCAGCA GCCCCAACAG 
CTGAATTCGC TTCATCAGAA CAACGCTGGA GCTGTCAATA GCTTCAATAA GGCCCAAATG 
CAAGCTCAAT CAGCTATTTC CATGCTGCCC AACAGCGGGG ATGAGTTTCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285687.1
CDS	487..3840
Translation	MQMAEAEQENGAGALKIATNAGATDRPAHQQLPLPVEDQQDEVLTPAEKGKFEYPPPPPPPTPVQAPLATKATALNASQEHDDDEANSEKWEDPCAPPPPPPLPTSAFLATGLGYLKLPAFKLKDALEKAITKLEANKRTLKASPESSRSIKNKNVVALEMLPRRSNPETIGDGSMLASTSTAVMLQTKKPAVIVEMERRCKIINLLAKQNQILESISGEAIPMHGPSKHLHEDEGLTLQVLSARASTPYTQPSSMLSCTAVSCDLEHDSPRKQVASKEAVPEQQSSQVQQKRPPSTGIHKPGSLRAPKAVRPTTAPVVSSKPVKSYTRSRLMDIRNGMFNALMHRSKESFVMPRIATCDDIELEGRLRRMNIWRTSDGTRFRTRSTTANLNMNNNNNNECMPAFFKNKNKPNLISDESIIQSQPPQPQTEFQDPAIVNQRRIGSGRLNHSKWGYNDEDYHSYHNGKSQHMEEVNSKNSKNMTVLQFFDNGEISSQPQRRPNTPVMGMSINRSENDTLHSNESSEDLSRANENYVKRVMSGFLVVSKPKSRDVEDRHHRRYRNQNEEPEWFSCGPTSRLDTIELCGFDEDEEKMLKEGNKNHGLGETERETSKQKMDHKYKWTHAEPMGRSKYMPKHDTNNNHNVENMNNVMATEHQQQKEEKRPGSGRSFQFDKFNQSQQNYESSSYVNHQQPPQTQPQQMQQQSNTNTNNSKFMSFFANEGNSSSSSLNEFFKQAINQGHGNNPEQPKSLGHIGQMPSVDQLEAKWRRNSLNNVGETANKQTDNFQKLIGSLSSAKPQSQAVGYDAISNFIMQQQQYQQQQQKQHLIIQQQQQHTAFLASLQLKAILGRADTQLLLLRLTKGEISKHGLLVQLANPRLTDMDREAITAVLQFTNTQQQQQQHKQQLDMLSSTVIASQLQNLHNLAIVQQTLAARQQPQHNPQTQAPHQLSQEDLQAHANVIMRNAVMKRKIEEQTSKLINGGAKHQAQQQYLNRGQQRQARPDANSNALLHALISGGGNNHASGYPMNGQPQKHHSNLRFGDNQNFQSFESNQPHFATQYKQQYQQSQQQHPHQQPQQLNSLHQNNAGAVNSFNKAQMQAQSAISMLPNSGDEFH

Target ORF information:

RefSeq Version	NM_001298758.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cup, transcript variant C (cup), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298758.1

ORF Insert Sequence:

ATGCAAATGG CCGAAGCTGA GCAAGAAAAT GGCGCTGGCG CCCTGAAAAT TGCCACCAAT 
GCGGGTGCAA CCGATCGTCC AGCGCATCAG CAGCTTCCGC TGCCAGTCGA AGATCAGCAG 
GATGAGGTGC TGACCCCCGC TGAGAAGGGC AAGTTCGAGT ACCCGCCCCC TCCACCGCCA 
CCAACTCCAG TCCAGGCTCC GCTAGCCACC AAGGCCACCG CTCTGAATGC CAGTCAAGAG 
CATGACGACG ACGAGGCCAA CTCCGAGAAG TGGGAGGATC CGTGCGCTCC GCCCCCGCCT 
CCTCCGCTGC CGACTAGCGC TTTCCTGGCC ACCGGACTGG GCTACCTCAA GCTGCCCGCC 
TTCAAGCTGA AGGACGCACT GGAGAAGGCC ATCACCAAGT TGGAGGCCAA CAAGCGGACG 
CTAAAGGCCA GCCCTGAGTC TTCTCGTTCC ATCAAAAACA AGAATGTGGT GGCCCTGGAG 
ATGTTACCCA GGCGCTCAAA CCCAGAAACG ATCGGCGATG GATCCATGTT GGCATCCACA 
TCCACGGCTG TTATGCTGCA GACCAAAAAG CCCGCCGTTA TTGTGGAGAT GGAGAGGCGT 
TGTAAGATCA TCAATTTACT GGCCAAACAG AATCAAATCC TCGAATCCAT TTCGGGGGAG 
GCCATTCCGA TGCATGGACC CTCGAAGCAT CTGCACGAGG ACGAGGGTCT GACCCTGCAA 
GTTCTCTCGG CCCGAGCATC CACACCGTAC ACACAGCCCT CCAGCATGTT GTCCTGCACG 
GCCGTTAGTT GTGACCTGGA GCACGATTCG CCGCGGAAGC AAGTGGCCAG CAAGGAGGCC 
GTGCCGGAGC AGCAGTCTAG TCAGGTGCAG CAAAAGCGTC CACCCAGCAC AGGCATTCAC 
AAGCCAGGAA GTCTGCGAGC ACCCAAGGCT GTTCGTCCTA CTACTGCTCC GGTGGTCAGC 
AGCAAGCCGG TCAAGAGCTA TACCCGCTCC CGCCTGATGG ACATTCGCAA CGGGATGTTC 
AATGCCCTGA TGCACAGGTC CAAGGAGAGC TTTGTGATGC CTCGCATCGC CACCTGCGAC 
GACATTGAGT TGGAGGGAAG GCTTCGCCGC ATGAATATTT GGCGCACCTC GGACGGCACA 
AGATTCCGGA CCCGCTCCAC CACAGCTAAC TTAAACATGA ACAACAACAA CAACAATGAG 
TGCATGCCTG CCTTCTTCAA AAACAAAAAT AAGCCGAACC TAATCAGTGA CGAGTCCATC 
ATCCAGAGCC AACCGCCCCA GCCGCAAACG GAGTTCCAAG ATCCGGCCAT TGTTAACCAA 
AGACGCATTG GCAGCGGTCG CTTAAACCAT TCCAAGTGGG GTTACAACGA CGAAGACTAC 
CACTCGTACC ACAATGGCAA GTCGCAGCAT ATGGAGGAGG TCAACTCGAA GAACAGCAAA 
AACATGACGG TGCTGCAGTT CTTCGACAAC GGCGAGATCA GCAGTCAGCC TCAAAGACGG 
CCGAACACTC CTGTGATGGG GATGTCTATC AATCGATCCG AGAACGACAC CCTGCACTCG 
AACGAGTCCA GTGAAGATTT GAGTCGCGCC AACGAGAACT ATGTAAAGCG CGTAATGTCT 
GGATTCCTGG TTGTATCGAA GCCCAAGTCA CGAGATGTCG AGGATAGACA TCACCGGCGG 
TACAGGAACC AGAACGAGGA GCCTGAGTGG TTTAGCTGTG GACCCACGTC TAGACTGGAC 
ACTATAGAGT TGTGTGGGTT CGATGAGGAC GAGGAGAAAA TGCTAAAGGA GGGCAACAAA 
AACCACGGAT TAGGCGAAAC AGAGCGAGAA ACGTCAAAAC AAAAGATGGA CCATAAGTAC 
AAATGGACGC ATGCGGAGCC GATGGGTCGA AGCAAATACA TGCCCAAACA CGACACTAAC 
AATAATCATA ACGTGGAGAA CATGAATAAT GTTATGGCTA CCGAGCATCA GCAACAAAAG 
GAAGAGAAAC GCCCAGGCAG TGGCCGTTCG TTTCAGTTTG ATAAGTTCAA TCAAAGCCAA 
CAGAATTACG AGAGTAGCAG TTATGTTAAT CACCAACAGC CGCCGCAAAC GCAGCCTCAG 
CAAATGCAGC AGCAGTCGAA CACAAACACG AACAACTCTA AATTCATGTC ATTCTTTGCC 
AATGAGGGGA ACTCCTCGTC TTCTTCGCTG AACGAGTTTT TCAAGCAGGC CATTAACCAG 
GGTCATGGCA ACAATCCGGA GCAGCCCAAA TCGTTGGGCC ACATCGGACA GATGCCTTCG 
GTGGATCAGC TGGAGGCCAA ATGGCGTCGA AACTCACTGA ACAATGTTGG CGAGACTGCC 
AACAAGCAGA CTGATAACTT CCAGAAGCTA ATTGGGTCTC TTTCTTCGGC TAAGCCACAG 
TCACAGGCGG TTGGCTATGA TGCCATCTCC AACTTTATAA TGCAGCAACA GCAGTACCAG 
CAGCAGCAAC AGAAGCAGCA CCTCATCATA CAGCAGCAGC AGCAACATAC CGCCTTTTTG 
GCGAGCCTGC AGCTGAAGGC GATCCTCGGC CGGGCCGACA CCCAATTGCT ACTGCTCCGT 
CTGACTAAAG GGGAAATATC CAAGCACGGA TTGCTGGTGC AGTTGGCCAA CCCACGCTTG 
ACGGACATGG ATCGCGAGGC CATCACTGCT GTATTGCAGT TCACCAACAC CCAGCAGCAG 
CAGCAGCAGC ACAAGCAGCA GCTGGACATG CTCTCAAGCA CGGTCATCGC CAGCCAGTTG 
CAGAATCTCC ACAACTTGGC CATTGTCCAG CAGACTCTTG CAGCCAGGCA GCAGCCGCAG 
CATAATCCAC AGACGCAGGC GCCGCACCAA CTGTCGCAGG AGGACTTGCA AGCGCATGCC 
AATGTGATAA TGCGAAACGC CGTAATGAAG CGAAAAATTG AGGAGCAGAC CTCGAAGCTT 
ATCAATGGCG GTGCCAAGCA TCAGGCACAG CAGCAATATC TAAACCGTGG CCAGCAACGC 
CAGGCTCGAC CAGATGCCAA CTCGAATGCC TTGCTCCATG CCTTGATCTC TGGCGGTGGC 
AATAACCACG CCTCTGGTTA TCCTATGAAT GGACAGCCGC AGAAGCATCA TTCCAATTTG 
AGATTCGGTG ATAACCAAAA CTTCCAATCT TTTGAGTCCA ACCAGCCGCA CTTCGCCACG 
CAGTACAAGC AGCAATACCA ACAGTCTCAG CAGCAACATC CCCATCAGCA GCCCCAACAG 
CTGAATTCGC TTCATCAGAA CAACGCTGGA GCTGTCAATA GCTTCAATAA GGCCCAAATG 
CAAGCTCAAT CAGCTATTTC CATGCTGCCC AACAGCGGGG ATGAGTTTCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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cup cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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