ed cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following ed gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ed cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa13703 NM_078745.3
Latest version!
Drosophila melanogaster echinoid (ed), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa13703 NM_001273084.1
Latest version!
Drosophila melanogaster echinoid (ed), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa16200 NM_001273085.1
Latest version!
Drosophila melanogaster echinoid (ed), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone 40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa13703
    Clone ID Related Accession (Same CDS sequence) NM_078745.3 , NM_001273084.1
    Accession Version NM_078745.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product echinoid, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_078745.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGGCGAA AAACTACCAC GAAAGGGACG GCAATAGTCA ATAGCAGGAG TGCGAGGAGA 
    GCAGCAACGA CTATATGCAA CTTTTTTCTC ATCGCACTCG TAGCTCTGAG TTCCACGACT
    CTGGCCGATG AATCCATTGA CACACGTGAG AATGCTGACT TAACCCTCAA GTGCCGGTTT
    AACGACAAGT ATGAGGCGAA CGATTTCTCT TTCTTCTGGA CCCGCTGGAC AGCGAATCCC
    GCCCAATTCG ATAACGTAGC CATCGGTGAA GTGCAGTTGA GTTCCGGCTA TAGACTCGAC
    TTTCAACCGG AGCGTGGCAT TTACGATCTG CAGATCAAGA ATGTGTCCTA TAACCGTGAC
    AATGGACGCT TCGAGTGCCG CATCAAGGCC AAGGGAACGG GCGCAGACGT CCATCAGGAG
    TTCCACAATC TCACGGTGCT CACGCCACCT CATCCACCCG TGATCTCACC CGGAAACATA
    GCCGTGGCCA CCGAGGACAA GCCCATGGAG CTGACATGCA GCAGCATCGG TGGTTCCCCC
    GATCCCACCA TAACTTGGTA TCGCGAGGGT TCGAATACGC CCCTGCCAGC GACGGTGTTA
    AAGGGCGGTA CCAAGGATCA GCCCACCAAT GCCACGCTAT CCATAATTCC TCGCAGAGAA
    GATGACGGTG CCAAGTACAA GTGTGTAGTC AGAAATCGTG CCATGAACGA GGGAAAGCGA
    TTGGAGGCCA CTGCCACATT GAATGTTAAC TATTACCCCC GAGTCGAGGT TGGACCCGAG
    AATCCTTTGC GGGTGGAACG TGACCGGACT GCCAAACTGG AGTGTAATGT GGATGCCAAG
    CCGAAGGTTC CCAATGTCCG TTGGAATCGC AATGGTCGGT TCATCAGCTC CTCATTAGTT
    CACACGATCC ATCGGGTGAG TGTTCAAGAT GCCGGCAAGT ATACCTGCAT TGCGGACAAC
    GGTCTTGGAA AGACCGGAGA ACAGGAGCTC ATCCTGGATA TCCTGTATCC ACCCATGGTC
    GTGATAGAGT CCAAGACGCG AGAGGCCGAA GAGGGCGATA CAGTGACCAT TCGCTGCAAT
    GTCACAGCCA ATCCTGCACC CGTGACCATT GAGTGGCTCA AGGAGAACAG TCCCGACTTC
    CGCTACAATG GCGACGTACT GACCCTAACT TCCGTGCGAG CCGATCATGC CGGAAACTAC
    ATCTGCCGTG CCGTGAACAT AATGCAGAGC CAGGGAATGG AGCGCAGTGA ACGAGTGGGT
    AACTCCACGG TGGCCCTGCT AGTTCGCCAT CGACCAGGAC AGGCTTACAT TACACCCAAT
    AAGCCCGTGG TTCATGTGGG TAATGGTGTC ACCTTGACCT GCTCCGCTAA TCCGCCAGGC
    TGGCCAGTGC CCCAGTACAG ATGGTTCAGG GATATGGATG GAGAGTTCTC CAGCACGCAG
    AAAATCCTTG CACAAGGCCC TCAGTACTCG ATACCCAAAG CTCACTTGGG TAACGAGGGT
    AAATACCATT GTCATGCTGT AAATGAACTG GGCATCGGAA TGATGGCCAC CATTGTCCTG
    GAGATCCACC AGCCTCCGCA GTTCTTGGCC AAGCTACAGC AGCATATGAC TCGCAGAGTG
    GCCGATACGG ATTATACGGT CACGTGCAGT GCCAAGGGAA AGCCAGCACC CAGTGTTAAA
    TGGCTGAAGG ACGCAGTGGA AATCCTGCCC GAGGAAAATC TCTACGAAGT GCAGACCAAT
    CCCGATCAGG GCCTCAACGG CATGGTCACG GTTCAGAGTC AACTTAAGTT CCGAGGAAAG
    GCCAGACCGA ATGGAAACGC TTTAGTGCCT GGAGATCGTG GCTTGTACAC CTGCTTGTAT
    CAGAACGAGG TCAACTCGGC CAACTCGTCC ATGCAACTGA GGATCGAACA TGAGCCCATT
    GTACTCCATC AGTATAACAA GGTGGCCTTT GATATCAGGG AGACAGCCGA GGTGGTTTGC
    AAGGTTCAGG CCTATCCCAA GCCCGAGTTC CAATGGCAGT TTGGCAATAA TCCATCGCCC
    CTGACCATGT CCTCCGATGG TCACTACGAG ATTAGTACCA CCACCGATAA CAACGACATC
    TATACGTCCG TGCTCAAGAT CAACTCGCTG ACTCATTCGG ATTACGGAGA ATACACCTGC
    CGGGTGGCCA ACACCCTGGA TACGATTCGA GCTCCCATTA GACTGCAGCA GAAAGGACCC
    CCAGAGAAAC CAACAAATCT GCGAGCCACC GAGGTGGGTC ACAATTACGT ATCACTGAGC
    TGGGATCCCG GATTCGATGG AGGTCTGAGC AAGACCAAGT TCTTTGTTAG CTATCGCCGT
    GTGGCCATGC CAAGGGAAGA ACAATTGATA CCGGATTGCG CCACTTTGGC CAACTCGAAT
    TCGGCTTGGG TGGAAGTCGA TTGCCAGCGG GATATTCCCT GCAAGGTGAC AGCCCTGGAG
    CAACACCAGA GTTATGCCTT TAAGGTCAAG GCTCTGAATC CCAAGAGCGA TTCACCGTAC
    TCGAGTGAAA TCATGGTGAC GACAAAGGTC AGCAGGATTC CGCCACCATT GCAGGTGACC
    TATGAGCCGA ATACTCGAAC TCTCGGCATC GATGTGGGGG CCACGTGCTT GAACCTGGTG
    GCCGTTGTGG AGTCCATGGT GAATGCAGAT TCTCCGATGG CCGCCTGGGA GGTGGTGACC
    ACCATGGATA ACCTGCAGCT GTCCGGAAAT GGACCCACCC ACAAGGAGAA GATCATCGAC
    AGAATAATTG GAGCGCGGCG AGTTGGCGGA GGACGTGCTC TGGGGCACAC CATCAGCGAG
    GACGAGGATG ACAATGGTTT GAACTCGCTG GCCCTCGAGG ACGAAAACAG TCCCACTGTG
    CGGGTCAAAT TGTGTCTGAG GACCAATCCA GAGCACTGCG GCGATTACAC GGACGCAGAA
    ATTGGCAGGC CGTATATAGC GGAGGCTAAT GCCCTGGCCA CGCCCACTCT CATTGCGATA
    ATCGTGTCCT GCGTGGTCTT TGCCCTCTTC GCTGGCCTAA TCCTGATGTT CTGCCGCTGC
    AAGCGCAATC AATCGAAGAA GAGCGCCGCC GCCAAAGATT ACGAGATGGA CTCGGTGCGT
    CCTTCGATTG TGGCCGCTCA GCAAAACCAG GCGCCACCAC CCTACTACCC CGCCTCTGGG
    CTGGACAACA AGGCATTGGA ACACTCAATG GATCTCGCCC TCAGCATGGA GGATCAGAAA
    ACGGCACTAT ACGCCACCCA GAATGGATAT AGCTATCATC CGGGAAGTGG GGTGGTGGGC
    GTTGGCATGG GCGGCGGCGT TGTGGGAGTG GGTGTTGGCG GCAGCGTGGT CAGCGGAATG
    GGCGGCGGAG TGGGCGGAAT CGGAGGCAGT GGCGTCGGCG TCAATGGAAT ACCTGGACTC
    AGCGCACACA CGATGCCCGG AAATGAATGG GTCAACATGG GCTACATGGA GAACAACTAT
    TCGAATTCGA ACAACGGCGG CAGTGTCAAC TCGCAGGACT CACTGTGGCA GGTGAAGATG
    TCAGCCGCGG CGGTGGGCAA CCAGCAGGGC ATGGTGCAGG CCCCAATGAA TCAGTATGTG
    GAACAGCAGC CCGCCTACGG ATACGATCCG TTGACCCATG GTGGCTATGG GGCGGTGGAC
    GACTACGCTC CCTATCCGCA CCTCACGGCC ACGCCCAGCC AGGTGGGCGA TGAGTACCAC
    AACTTGCGCA ACAGCCAGAA TCCGTCCCGG CAGGACTACT GCAGCGATCC CTATGCATCC
    GTGCAAAAGC CCAAAAAGCG AGTCGATCAG CATTTAGATT CACCATATCA CGACGTAAGC
    GGTCTGCCCA ATCCCTACAA CATGGAGCAT TTGGAACAGG ACGAGGTCCT TCCCCCGCAG
    CAGCACATGT CCTTGAGCTA CGACGACAGC TTCGAGGGCG AATATTCGAC AACGCCGAAT
    GCCAGAAACC GTCGCGTCAT ACGCGAGATT ATTGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_523469.2
    CDS433..4431
    Translation

    Target ORF information:

    RefSeq Version NM_078745.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster echinoid (ed), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_078745.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGGCGAA AAACTACCAC GAAAGGGACG GCAATAGTCA ATAGCAGGAG TGCGAGGAGA 
    GCAGCAACGA CTATATGCAA CTTTTTTCTC ATCGCACTCG TAGCTCTGAG TTCCACGACT
    CTGGCCGATG AATCCATTGA CACACGTGAG AATGCTGACT TAACCCTCAA GTGCCGGTTT
    AACGACAAGT ATGAGGCGAA CGATTTCTCT TTCTTCTGGA CCCGCTGGAC AGCGAATCCC
    GCCCAATTCG ATAACGTAGC CATCGGTGAA GTGCAGTTGA GTTCCGGCTA TAGACTCGAC
    TTTCAACCGG AGCGTGGCAT TTACGATCTG CAGATCAAGA ATGTGTCCTA TAACCGTGAC
    AATGGACGCT TCGAGTGCCG CATCAAGGCC AAGGGAACGG GCGCAGACGT CCATCAGGAG
    TTCCACAATC TCACGGTGCT CACGCCACCT CATCCACCCG TGATCTCACC CGGAAACATA
    GCCGTGGCCA CCGAGGACAA GCCCATGGAG CTGACATGCA GCAGCATCGG TGGTTCCCCC
    GATCCCACCA TAACTTGGTA TCGCGAGGGT TCGAATACGC CCCTGCCAGC GACGGTGTTA
    AAGGGCGGTA CCAAGGATCA GCCCACCAAT GCCACGCTAT CCATAATTCC TCGCAGAGAA
    GATGACGGTG CCAAGTACAA GTGTGTAGTC AGAAATCGTG CCATGAACGA GGGAAAGCGA
    TTGGAGGCCA CTGCCACATT GAATGTTAAC TATTACCCCC GAGTCGAGGT TGGACCCGAG
    AATCCTTTGC GGGTGGAACG TGACCGGACT GCCAAACTGG AGTGTAATGT GGATGCCAAG
    CCGAAGGTTC CCAATGTCCG TTGGAATCGC AATGGTCGGT TCATCAGCTC CTCATTAGTT
    CACACGATCC ATCGGGTGAG TGTTCAAGAT GCCGGCAAGT ATACCTGCAT TGCGGACAAC
    GGTCTTGGAA AGACCGGAGA ACAGGAGCTC ATCCTGGATA TCCTGTATCC ACCCATGGTC
    GTGATAGAGT CCAAGACGCG AGAGGCCGAA GAGGGCGATA CAGTGACCAT TCGCTGCAAT
    GTCACAGCCA ATCCTGCACC CGTGACCATT GAGTGGCTCA AGGAGAACAG TCCCGACTTC
    CGCTACAATG GCGACGTACT GACCCTAACT TCCGTGCGAG CCGATCATGC CGGAAACTAC
    ATCTGCCGTG CCGTGAACAT AATGCAGAGC CAGGGAATGG AGCGCAGTGA ACGAGTGGGT
    AACTCCACGG TGGCCCTGCT AGTTCGCCAT CGACCAGGAC AGGCTTACAT TACACCCAAT
    AAGCCCGTGG TTCATGTGGG TAATGGTGTC ACCTTGACCT GCTCCGCTAA TCCGCCAGGC
    TGGCCAGTGC CCCAGTACAG ATGGTTCAGG GATATGGATG GAGAGTTCTC CAGCACGCAG
    AAAATCCTTG CACAAGGCCC TCAGTACTCG ATACCCAAAG CTCACTTGGG TAACGAGGGT
    AAATACCATT GTCATGCTGT AAATGAACTG GGCATCGGAA TGATGGCCAC CATTGTCCTG
    GAGATCCACC AGCCTCCGCA GTTCTTGGCC AAGCTACAGC AGCATATGAC TCGCAGAGTG
    GCCGATACGG ATTATACGGT CACGTGCAGT GCCAAGGGAA AGCCAGCACC CAGTGTTAAA
    TGGCTGAAGG ACGCAGTGGA AATCCTGCCC GAGGAAAATC TCTACGAAGT GCAGACCAAT
    CCCGATCAGG GCCTCAACGG CATGGTCACG GTTCAGAGTC AACTTAAGTT CCGAGGAAAG
    GCCAGACCGA ATGGAAACGC TTTAGTGCCT GGAGATCGTG GCTTGTACAC CTGCTTGTAT
    CAGAACGAGG TCAACTCGGC CAACTCGTCC ATGCAACTGA GGATCGAACA TGAGCCCATT
    GTACTCCATC AGTATAACAA GGTGGCCTTT GATATCAGGG AGACAGCCGA GGTGGTTTGC
    AAGGTTCAGG CCTATCCCAA GCCCGAGTTC CAATGGCAGT TTGGCAATAA TCCATCGCCC
    CTGACCATGT CCTCCGATGG TCACTACGAG ATTAGTACCA CCACCGATAA CAACGACATC
    TATACGTCCG TGCTCAAGAT CAACTCGCTG ACTCATTCGG ATTACGGAGA ATACACCTGC
    CGGGTGGCCA ACACCCTGGA TACGATTCGA GCTCCCATTA GACTGCAGCA GAAAGGACCC
    CCAGAGAAAC CAACAAATCT GCGAGCCACC GAGGTGGGTC ACAATTACGT ATCACTGAGC
    TGGGATCCCG GATTCGATGG AGGTCTGAGC AAGACCAAGT TCTTTGTTAG CTATCGCCGT
    GTGGCCATGC CAAGGGAAGA ACAATTGATA CCGGATTGCG CCACTTTGGC CAACTCGAAT
    TCGGCTTGGG TGGAAGTCGA TTGCCAGCGG GATATTCCCT GCAAGGTGAC AGCCCTGGAG
    CAACACCAGA GTTATGCCTT TAAGGTCAAG GCTCTGAATC CCAAGAGCGA TTCACCGTAC
    TCGAGTGAAA TCATGGTGAC GACAAAGGTC AGCAGGATTC CGCCACCATT GCAGGTGACC
    TATGAGCCGA ATACTCGAAC TCTCGGCATC GATGTGGGGG CCACGTGCTT GAACCTGGTG
    GCCGTTGTGG AGTCCATGGT GAATGCAGAT TCTCCGATGG CCGCCTGGGA GGTGGTGACC
    ACCATGGATA ACCTGCAGCT GTCCGGAAAT GGACCCACCC ACAAGGAGAA GATCATCGAC
    AGAATAATTG GAGCGCGGCG AGTTGGCGGA GGACGTGCTC TGGGGCACAC CATCAGCGAG
    GACGAGGATG ACAATGGTTT GAACTCGCTG GCCCTCGAGG ACGAAAACAG TCCCACTGTG
    CGGGTCAAAT TGTGTCTGAG GACCAATCCA GAGCACTGCG GCGATTACAC GGACGCAGAA
    ATTGGCAGGC CGTATATAGC GGAGGCTAAT GCCCTGGCCA CGCCCACTCT CATTGCGATA
    ATCGTGTCCT GCGTGGTCTT TGCCCTCTTC GCTGGCCTAA TCCTGATGTT CTGCCGCTGC
    AAGCGCAATC AATCGAAGAA GAGCGCCGCC GCCAAAGATT ACGAGATGGA CTCGGTGCGT
    CCTTCGATTG TGGCCGCTCA GCAAAACCAG GCGCCACCAC CCTACTACCC CGCCTCTGGG
    CTGGACAACA AGGCATTGGA ACACTCAATG GATCTCGCCC TCAGCATGGA GGATCAGAAA
    ACGGCACTAT ACGCCACCCA GAATGGATAT AGCTATCATC CGGGAAGTGG GGTGGTGGGC
    GTTGGCATGG GCGGCGGCGT TGTGGGAGTG GGTGTTGGCG GCAGCGTGGT CAGCGGAATG
    GGCGGCGGAG TGGGCGGAAT CGGAGGCAGT GGCGTCGGCG TCAATGGAAT ACCTGGACTC
    AGCGCACACA CGATGCCCGG AAATGAATGG GTCAACATGG GCTACATGGA GAACAACTAT
    TCGAATTCGA ACAACGGCGG CAGTGTCAAC TCGCAGGACT CACTGTGGCA GGTGAAGATG
    TCAGCCGCGG CGGTGGGCAA CCAGCAGGGC ATGGTGCAGG CCCCAATGAA TCAGTATGTG
    GAACAGCAGC CCGCCTACGG ATACGATCCG TTGACCCATG GTGGCTATGG GGCGGTGGAC
    GACTACGCTC CCTATCCGCA CCTCACGGCC ACGCCCAGCC AGGTGGGCGA TGAGTACCAC
    AACTTGCGCA ACAGCCAGAA TCCGTCCCGG CAGGACTACT GCAGCGATCC CTATGCATCC
    GTGCAAAAGC CCAAAAAGCG AGTCGATCAG CATTTAGATT CACCATATCA CGACGTAAGC
    GGTCTGCCCA ATCCCTACAA CATGGAGCAT TTGGAACAGG ACGAGGTCCT TCCCCCGCAG
    CAGCACATGT CCTTGAGCTA CGACGACAGC TTCGAGGGCG AATATTCGAC AACGCCGAAT
    GCCAGAAACC GTCGCGTCAT ACGCGAGATT ATTGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa13703
    Clone ID Related Accession (Same CDS sequence) NM_078745.3 , NM_001273084.1
    Accession Version NM_001273084.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product echinoid, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGGCGAA AAACTACCAC GAAAGGGACG GCAATAGTCA ATAGCAGGAG TGCGAGGAGA 
    GCAGCAACGA CTATATGCAA CTTTTTTCTC ATCGCACTCG TAGCTCTGAG TTCCACGACT
    CTGGCCGATG AATCCATTGA CACACGTGAG AATGCTGACT TAACCCTCAA GTGCCGGTTT
    AACGACAAGT ATGAGGCGAA CGATTTCTCT TTCTTCTGGA CCCGCTGGAC AGCGAATCCC
    GCCCAATTCG ATAACGTAGC CATCGGTGAA GTGCAGTTGA GTTCCGGCTA TAGACTCGAC
    TTTCAACCGG AGCGTGGCAT TTACGATCTG CAGATCAAGA ATGTGTCCTA TAACCGTGAC
    AATGGACGCT TCGAGTGCCG CATCAAGGCC AAGGGAACGG GCGCAGACGT CCATCAGGAG
    TTCCACAATC TCACGGTGCT CACGCCACCT CATCCACCCG TGATCTCACC CGGAAACATA
    GCCGTGGCCA CCGAGGACAA GCCCATGGAG CTGACATGCA GCAGCATCGG TGGTTCCCCC
    GATCCCACCA TAACTTGGTA TCGCGAGGGT TCGAATACGC CCCTGCCAGC GACGGTGTTA
    AAGGGCGGTA CCAAGGATCA GCCCACCAAT GCCACGCTAT CCATAATTCC TCGCAGAGAA
    GATGACGGTG CCAAGTACAA GTGTGTAGTC AGAAATCGTG CCATGAACGA GGGAAAGCGA
    TTGGAGGCCA CTGCCACATT GAATGTTAAC TATTACCCCC GAGTCGAGGT TGGACCCGAG
    AATCCTTTGC GGGTGGAACG TGACCGGACT GCCAAACTGG AGTGTAATGT GGATGCCAAG
    CCGAAGGTTC CCAATGTCCG TTGGAATCGC AATGGTCGGT TCATCAGCTC CTCATTAGTT
    CACACGATCC ATCGGGTGAG TGTTCAAGAT GCCGGCAAGT ATACCTGCAT TGCGGACAAC
    GGTCTTGGAA AGACCGGAGA ACAGGAGCTC ATCCTGGATA TCCTGTATCC ACCCATGGTC
    GTGATAGAGT CCAAGACGCG AGAGGCCGAA GAGGGCGATA CAGTGACCAT TCGCTGCAAT
    GTCACAGCCA ATCCTGCACC CGTGACCATT GAGTGGCTCA AGGAGAACAG TCCCGACTTC
    CGCTACAATG GCGACGTACT GACCCTAACT TCCGTGCGAG CCGATCATGC CGGAAACTAC
    ATCTGCCGTG CCGTGAACAT AATGCAGAGC CAGGGAATGG AGCGCAGTGA ACGAGTGGGT
    AACTCCACGG TGGCCCTGCT AGTTCGCCAT CGACCAGGAC AGGCTTACAT TACACCCAAT
    AAGCCCGTGG TTCATGTGGG TAATGGTGTC ACCTTGACCT GCTCCGCTAA TCCGCCAGGC
    TGGCCAGTGC CCCAGTACAG ATGGTTCAGG GATATGGATG GAGAGTTCTC CAGCACGCAG
    AAAATCCTTG CACAAGGCCC TCAGTACTCG ATACCCAAAG CTCACTTGGG TAACGAGGGT
    AAATACCATT GTCATGCTGT AAATGAACTG GGCATCGGAA TGATGGCCAC CATTGTCCTG
    GAGATCCACC AGCCTCCGCA GTTCTTGGCC AAGCTACAGC AGCATATGAC TCGCAGAGTG
    GCCGATACGG ATTATACGGT CACGTGCAGT GCCAAGGGAA AGCCAGCACC CAGTGTTAAA
    TGGCTGAAGG ACGCAGTGGA AATCCTGCCC GAGGAAAATC TCTACGAAGT GCAGACCAAT
    CCCGATCAGG GCCTCAACGG CATGGTCACG GTTCAGAGTC AACTTAAGTT CCGAGGAAAG
    GCCAGACCGA ATGGAAACGC TTTAGTGCCT GGAGATCGTG GCTTGTACAC CTGCTTGTAT
    CAGAACGAGG TCAACTCGGC CAACTCGTCC ATGCAACTGA GGATCGAACA TGAGCCCATT
    GTACTCCATC AGTATAACAA GGTGGCCTTT GATATCAGGG AGACAGCCGA GGTGGTTTGC
    AAGGTTCAGG CCTATCCCAA GCCCGAGTTC CAATGGCAGT TTGGCAATAA TCCATCGCCC
    CTGACCATGT CCTCCGATGG TCACTACGAG ATTAGTACCA CCACCGATAA CAACGACATC
    TATACGTCCG TGCTCAAGAT CAACTCGCTG ACTCATTCGG ATTACGGAGA ATACACCTGC
    CGGGTGGCCA ACACCCTGGA TACGATTCGA GCTCCCATTA GACTGCAGCA GAAAGGACCC
    CCAGAGAAAC CAACAAATCT GCGAGCCACC GAGGTGGGTC ACAATTACGT ATCACTGAGC
    TGGGATCCCG GATTCGATGG AGGTCTGAGC AAGACCAAGT TCTTTGTTAG CTATCGCCGT
    GTGGCCATGC CAAGGGAAGA ACAATTGATA CCGGATTGCG CCACTTTGGC CAACTCGAAT
    TCGGCTTGGG TGGAAGTCGA TTGCCAGCGG GATATTCCCT GCAAGGTGAC AGCCCTGGAG
    CAACACCAGA GTTATGCCTT TAAGGTCAAG GCTCTGAATC CCAAGAGCGA TTCACCGTAC
    TCGAGTGAAA TCATGGTGAC GACAAAGGTC AGCAGGATTC CGCCACCATT GCAGGTGACC
    TATGAGCCGA ATACTCGAAC TCTCGGCATC GATGTGGGGG CCACGTGCTT GAACCTGGTG
    GCCGTTGTGG AGTCCATGGT GAATGCAGAT TCTCCGATGG CCGCCTGGGA GGTGGTGACC
    ACCATGGATA ACCTGCAGCT GTCCGGAAAT GGACCCACCC ACAAGGAGAA GATCATCGAC
    AGAATAATTG GAGCGCGGCG AGTTGGCGGA GGACGTGCTC TGGGGCACAC CATCAGCGAG
    GACGAGGATG ACAATGGTTT GAACTCGCTG GCCCTCGAGG ACGAAAACAG TCCCACTGTG
    CGGGTCAAAT TGTGTCTGAG GACCAATCCA GAGCACTGCG GCGATTACAC GGACGCAGAA
    ATTGGCAGGC CGTATATAGC GGAGGCTAAT GCCCTGGCCA CGCCCACTCT CATTGCGATA
    ATCGTGTCCT GCGTGGTCTT TGCCCTCTTC GCTGGCCTAA TCCTGATGTT CTGCCGCTGC
    AAGCGCAATC AATCGAAGAA GAGCGCCGCC GCCAAAGATT ACGAGATGGA CTCGGTGCGT
    CCTTCGATTG TGGCCGCTCA GCAAAACCAG GCGCCACCAC CCTACTACCC CGCCTCTGGG
    CTGGACAACA AGGCATTGGA ACACTCAATG GATCTCGCCC TCAGCATGGA GGATCAGAAA
    ACGGCACTAT ACGCCACCCA GAATGGATAT AGCTATCATC CGGGAAGTGG GGTGGTGGGC
    GTTGGCATGG GCGGCGGCGT TGTGGGAGTG GGTGTTGGCG GCAGCGTGGT CAGCGGAATG
    GGCGGCGGAG TGGGCGGAAT CGGAGGCAGT GGCGTCGGCG TCAATGGAAT ACCTGGACTC
    AGCGCACACA CGATGCCCGG AAATGAATGG GTCAACATGG GCTACATGGA GAACAACTAT
    TCGAATTCGA ACAACGGCGG CAGTGTCAAC TCGCAGGACT CACTGTGGCA GGTGAAGATG
    TCAGCCGCGG CGGTGGGCAA CCAGCAGGGC ATGGTGCAGG CCCCAATGAA TCAGTATGTG
    GAACAGCAGC CCGCCTACGG ATACGATCCG TTGACCCATG GTGGCTATGG GGCGGTGGAC
    GACTACGCTC CCTATCCGCA CCTCACGGCC ACGCCCAGCC AGGTGGGCGA TGAGTACCAC
    AACTTGCGCA ACAGCCAGAA TCCGTCCCGG CAGGACTACT GCAGCGATCC CTATGCATCC
    GTGCAAAAGC CCAAAAAGCG AGTCGATCAG CATTTAGATT CACCATATCA CGACGTAAGC
    GGTCTGCCCA ATCCCTACAA CATGGAGCAT TTGGAACAGG ACGAGGTCCT TCCCCCGCAG
    CAGCACATGT CCTTGAGCTA CGACGACAGC TTCGAGGGCG AATATTCGAC AACGCCGAAT
    GCCAGAAACC GTCGCGTCAT ACGCGAGATT ATTGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260013.1
    CDS433..4431
    Translation

    Target ORF information:

    RefSeq Version NM_001273084.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster echinoid (ed), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273084.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGGCGAA AAACTACCAC GAAAGGGACG GCAATAGTCA ATAGCAGGAG TGCGAGGAGA 
    GCAGCAACGA CTATATGCAA CTTTTTTCTC ATCGCACTCG TAGCTCTGAG TTCCACGACT
    CTGGCCGATG AATCCATTGA CACACGTGAG AATGCTGACT TAACCCTCAA GTGCCGGTTT
    AACGACAAGT ATGAGGCGAA CGATTTCTCT TTCTTCTGGA CCCGCTGGAC AGCGAATCCC
    GCCCAATTCG ATAACGTAGC CATCGGTGAA GTGCAGTTGA GTTCCGGCTA TAGACTCGAC
    TTTCAACCGG AGCGTGGCAT TTACGATCTG CAGATCAAGA ATGTGTCCTA TAACCGTGAC
    AATGGACGCT TCGAGTGCCG CATCAAGGCC AAGGGAACGG GCGCAGACGT CCATCAGGAG
    TTCCACAATC TCACGGTGCT CACGCCACCT CATCCACCCG TGATCTCACC CGGAAACATA
    GCCGTGGCCA CCGAGGACAA GCCCATGGAG CTGACATGCA GCAGCATCGG TGGTTCCCCC
    GATCCCACCA TAACTTGGTA TCGCGAGGGT TCGAATACGC CCCTGCCAGC GACGGTGTTA
    AAGGGCGGTA CCAAGGATCA GCCCACCAAT GCCACGCTAT CCATAATTCC TCGCAGAGAA
    GATGACGGTG CCAAGTACAA GTGTGTAGTC AGAAATCGTG CCATGAACGA GGGAAAGCGA
    TTGGAGGCCA CTGCCACATT GAATGTTAAC TATTACCCCC GAGTCGAGGT TGGACCCGAG
    AATCCTTTGC GGGTGGAACG TGACCGGACT GCCAAACTGG AGTGTAATGT GGATGCCAAG
    CCGAAGGTTC CCAATGTCCG TTGGAATCGC AATGGTCGGT TCATCAGCTC CTCATTAGTT
    CACACGATCC ATCGGGTGAG TGTTCAAGAT GCCGGCAAGT ATACCTGCAT TGCGGACAAC
    GGTCTTGGAA AGACCGGAGA ACAGGAGCTC ATCCTGGATA TCCTGTATCC ACCCATGGTC
    GTGATAGAGT CCAAGACGCG AGAGGCCGAA GAGGGCGATA CAGTGACCAT TCGCTGCAAT
    GTCACAGCCA ATCCTGCACC CGTGACCATT GAGTGGCTCA AGGAGAACAG TCCCGACTTC
    CGCTACAATG GCGACGTACT GACCCTAACT TCCGTGCGAG CCGATCATGC CGGAAACTAC
    ATCTGCCGTG CCGTGAACAT AATGCAGAGC CAGGGAATGG AGCGCAGTGA ACGAGTGGGT
    AACTCCACGG TGGCCCTGCT AGTTCGCCAT CGACCAGGAC AGGCTTACAT TACACCCAAT
    AAGCCCGTGG TTCATGTGGG TAATGGTGTC ACCTTGACCT GCTCCGCTAA TCCGCCAGGC
    TGGCCAGTGC CCCAGTACAG ATGGTTCAGG GATATGGATG GAGAGTTCTC CAGCACGCAG
    AAAATCCTTG CACAAGGCCC TCAGTACTCG ATACCCAAAG CTCACTTGGG TAACGAGGGT
    AAATACCATT GTCATGCTGT AAATGAACTG GGCATCGGAA TGATGGCCAC CATTGTCCTG
    GAGATCCACC AGCCTCCGCA GTTCTTGGCC AAGCTACAGC AGCATATGAC TCGCAGAGTG
    GCCGATACGG ATTATACGGT CACGTGCAGT GCCAAGGGAA AGCCAGCACC CAGTGTTAAA
    TGGCTGAAGG ACGCAGTGGA AATCCTGCCC GAGGAAAATC TCTACGAAGT GCAGACCAAT
    CCCGATCAGG GCCTCAACGG CATGGTCACG GTTCAGAGTC AACTTAAGTT CCGAGGAAAG
    GCCAGACCGA ATGGAAACGC TTTAGTGCCT GGAGATCGTG GCTTGTACAC CTGCTTGTAT
    CAGAACGAGG TCAACTCGGC CAACTCGTCC ATGCAACTGA GGATCGAACA TGAGCCCATT
    GTACTCCATC AGTATAACAA GGTGGCCTTT GATATCAGGG AGACAGCCGA GGTGGTTTGC
    AAGGTTCAGG CCTATCCCAA GCCCGAGTTC CAATGGCAGT TTGGCAATAA TCCATCGCCC
    CTGACCATGT CCTCCGATGG TCACTACGAG ATTAGTACCA CCACCGATAA CAACGACATC
    TATACGTCCG TGCTCAAGAT CAACTCGCTG ACTCATTCGG ATTACGGAGA ATACACCTGC
    CGGGTGGCCA ACACCCTGGA TACGATTCGA GCTCCCATTA GACTGCAGCA GAAAGGACCC
    CCAGAGAAAC CAACAAATCT GCGAGCCACC GAGGTGGGTC ACAATTACGT ATCACTGAGC
    TGGGATCCCG GATTCGATGG AGGTCTGAGC AAGACCAAGT TCTTTGTTAG CTATCGCCGT
    GTGGCCATGC CAAGGGAAGA ACAATTGATA CCGGATTGCG CCACTTTGGC CAACTCGAAT
    TCGGCTTGGG TGGAAGTCGA TTGCCAGCGG GATATTCCCT GCAAGGTGAC AGCCCTGGAG
    CAACACCAGA GTTATGCCTT TAAGGTCAAG GCTCTGAATC CCAAGAGCGA TTCACCGTAC
    TCGAGTGAAA TCATGGTGAC GACAAAGGTC AGCAGGATTC CGCCACCATT GCAGGTGACC
    TATGAGCCGA ATACTCGAAC TCTCGGCATC GATGTGGGGG CCACGTGCTT GAACCTGGTG
    GCCGTTGTGG AGTCCATGGT GAATGCAGAT TCTCCGATGG CCGCCTGGGA GGTGGTGACC
    ACCATGGATA ACCTGCAGCT GTCCGGAAAT GGACCCACCC ACAAGGAGAA GATCATCGAC
    AGAATAATTG GAGCGCGGCG AGTTGGCGGA GGACGTGCTC TGGGGCACAC CATCAGCGAG
    GACGAGGATG ACAATGGTTT GAACTCGCTG GCCCTCGAGG ACGAAAACAG TCCCACTGTG
    CGGGTCAAAT TGTGTCTGAG GACCAATCCA GAGCACTGCG GCGATTACAC GGACGCAGAA
    ATTGGCAGGC CGTATATAGC GGAGGCTAAT GCCCTGGCCA CGCCCACTCT CATTGCGATA
    ATCGTGTCCT GCGTGGTCTT TGCCCTCTTC GCTGGCCTAA TCCTGATGTT CTGCCGCTGC
    AAGCGCAATC AATCGAAGAA GAGCGCCGCC GCCAAAGATT ACGAGATGGA CTCGGTGCGT
    CCTTCGATTG TGGCCGCTCA GCAAAACCAG GCGCCACCAC CCTACTACCC CGCCTCTGGG
    CTGGACAACA AGGCATTGGA ACACTCAATG GATCTCGCCC TCAGCATGGA GGATCAGAAA
    ACGGCACTAT ACGCCACCCA GAATGGATAT AGCTATCATC CGGGAAGTGG GGTGGTGGGC
    GTTGGCATGG GCGGCGGCGT TGTGGGAGTG GGTGTTGGCG GCAGCGTGGT CAGCGGAATG
    GGCGGCGGAG TGGGCGGAAT CGGAGGCAGT GGCGTCGGCG TCAATGGAAT ACCTGGACTC
    AGCGCACACA CGATGCCCGG AAATGAATGG GTCAACATGG GCTACATGGA GAACAACTAT
    TCGAATTCGA ACAACGGCGG CAGTGTCAAC TCGCAGGACT CACTGTGGCA GGTGAAGATG
    TCAGCCGCGG CGGTGGGCAA CCAGCAGGGC ATGGTGCAGG CCCCAATGAA TCAGTATGTG
    GAACAGCAGC CCGCCTACGG ATACGATCCG TTGACCCATG GTGGCTATGG GGCGGTGGAC
    GACTACGCTC CCTATCCGCA CCTCACGGCC ACGCCCAGCC AGGTGGGCGA TGAGTACCAC
    AACTTGCGCA ACAGCCAGAA TCCGTCCCGG CAGGACTACT GCAGCGATCC CTATGCATCC
    GTGCAAAAGC CCAAAAAGCG AGTCGATCAG CATTTAGATT CACCATATCA CGACGTAAGC
    GGTCTGCCCA ATCCCTACAA CATGGAGCAT TTGGAACAGG ACGAGGTCCT TCCCCCGCAG
    CAGCACATGT CCTTGAGCTA CGACGACAGC TTCGAGGGCG AATATTCGAC AACGCCGAAT
    GCCAGAAACC GTCGCGTCAT ACGCGAGATT ATTGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16200
    Clone ID Related Accession (Same CDS sequence) NM_001273085.1
    Accession Version NM_001273085.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product echinoid, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGGCGAA AAACTACCAC GAAAGGGACG GCAATAGTCA ATAGCAGGAG TGCGAGGAGA 
    GCAGCAACGA CTATATGCAA CTTTTTTCTC ATCGCACTCG TAGCTCTGAG TTCCACGACT
    CTGGCCGATG AATCCATTGA CACACGTGAG AATGCTGACT TAACCCTCAA GTGCCGGTTT
    AACGACAAGT ATGAGGCGAA CGATTTCTCT TTCTTCTGGA CCCGCTGGAC AGCGAATCCC
    GCCCAATTCG ATAACGTAGC CATCGGTGAA GTGCAGTTGA GTTCCGGCTA TAGACTCGAC
    TTTCAACCGG AGCGTGGCAT TTACGATCTG CAGATCAAGA ATGTGTCCTA TAACCGTGAC
    AATGGACGCT TCGAGTGCCG CATCAAGGCC AAGGGAACGG GCGCAGACGT CCATCAGGAG
    TTCCACAATC TCACGGTGCT CACGCCACCT CATCCACCCG TGATCTCACC CGGAAACATA
    GCCGTGGCCA CCGAGGACAA GCCCATGGAG CTGACATGCA GCAGCATCGG TGGTTCCCCC
    GATCCCACCA TAACTTGGTA TCGCGAGGGT TCGAATACGC CCCTGCCAGC GACGGTGTTA
    AAGGGCGGTA CCAAGGATCA GCCCACCAAT GCCACGCTAT CCATAATTCC TCGCAGAGAA
    GATGACGGTG CCAAGTACAA GTGTGTAGTC AGAAATCGTG CCATGAACGA GGGAAAGCGA
    TTGGAGGCCA CTGCCACATT GAATGTTAAC TATTACCCCC GAGTCGAGGT TGGACCCGAG
    AATCCTTTGC GGGTGGAACG TGACCGGACT GCCAAACTGG AGTGTAATGT GGATGCCAAG
    CCGAAGGTTC CCAATGTCCG TTGGAATCGC AATGGTCGGT TCATCAGCTC CTCATTAGTT
    CACACGATCC ATCGGGTGAG TGTTCAAGAT GCCGGCAAGT ATACCTGCAT TGCGGACAAC
    GGTCTTGGAA AGACCGGAGA ACAGGAGCTC ATCCTGGATA TCCTGTATCC ACCCATGGTC
    GTGATAGAGT CCAAGACGCG AGAGGCCGAA GAGGGCGATA CAGTGACCAT TCGCTGCAAT
    GTCACAGCCA ATCCTGCACC CGTGACCATT GAGTGGCTCA AGGAGAACAG TCCCGACTTC
    CGCTACAATG GCGACGTACT GACCCTAACT TCCGTGCGAG CCGATCATGC CGGAAACTAC
    ATCTGCCGTG CCGTGAACAT AATGCAGAGC CAGGGAATGG AGCGCAGTGA ACGAGTGGGT
    AACTCCACGG TGGCCCTGCT AGTTCGCCAT CGACCAGGAC AGGCTTACAT TACACCCAAT
    AAGCCCGTGG TTCATGTGGG TAATGGTGTC ACCTTGACCT GCTCCGCTAA TCCGCCAGGC
    TGGCCAGTGC CCCAGTACAG ATGGTTCAGG GATATGGATG GAGAGTTCTC CAGCACGCAG
    AAAATCCTTG CACAAGGCCC TCAGTACTCG ATACCCAAAG CTCACTTGGG TAACGAGGGT
    AAATACCATT GTCATGCTGT AAATGAACTG GGCATCGGAA TGATGGCCAC CATTGTCCTG
    GAGATCCACC AGCCTCCGCA GTTCTTGGCC AAGCTACAGC AGCATATGAC TCGCAGAGTG
    GCCGATACGG ATTATACGGT CACGTGCAGT GCCAAGGGAA AGCCAGCACC CAGTGTTAAA
    TGGCTGAAGG ACGCAGTGGA AATCCTGCCC GAGGAAAATC TCTACGAAGT GCAGACCAAT
    CCCGATCAGG GCCTCAACGG CATGGTCACG GTTCAGAGTC AACTTAAGTT CCGAGGAAAG
    GCCAGACCGA ATGGAAACGC TTTAGTGCCT GGAGATCGTG GCTTGTACAC CTGCTTGTAT
    CAGAACGAGG TCAACTCGGC CAACTCGTCC ATGCAACTGA GGATCGAACA TGAGCCCATT
    GTACTCCATC AGTATAACAA GGTGGCCTTT GATATCAGGG AGACAGCCGA GGTGGTTTGC
    AAGGTTCAGG CCTATCCCAA GCCCGAGTTC CAATGGCAGT TTGGCAATAA TCCATCGCCC
    CTGACCATGT CCTCCGATGG TCACTACGAG ATTAGTACCA CCACCGATAA CAACGACATC
    TATACGTCCG TGCTCAAGAT CAACTCGCTG ACTCATTCGG ATTACGGAGA ATACACCTGC
    CGGGTGGCCA ACACCCTGGA TACGATTCGA GCTCCCATTA GACTGCAGCA GAAAGGACCC
    CCAGAGAAAC CAACAAATCT GCGAGCCACC GAGGTGGGTC ACAATTACGT ATCACTGAGC
    TGGGATCCCG GATTCGATGG AGGTCTGAGC AAGACCAAGT TCTTTGTTAG CTATCGCCGT
    GTGGCCATGC CAAGGGAAGA ACAATTGATA CCGGATTGCG CCACTTTGGC CAACTCGAAT
    TCGGCTTGGG TGGAAGTCGA TTGCCAGCGG GATATTCCCT GCAAGGTGAC AGCCCTGGAG
    CAACACCAGA GTTATGCCTT TAAGGTCAAG GCTCTGAATC CCAAGAGCGA TTCACCGTAC
    TCGAGTGAAA TCATGGTGAC GACAAAGGTC AGCAGGATTC CGCCACCATT GCAGGTGACC
    TATGAGCCGA ATACTCGAAC TCTCGGCATC GATGTGGGGG CCACGTGCTT GAACCTGGTG
    GCCGTTGTGG AGTCCATGGT GAATGCAGAT TCTCCGATGG CCGCCTGGGA GGTGGTGACC
    ACCATGGATA ACCTGCAGCT GTCCGGAAAT GGACCCACCC ACAAGGAGAA GATCATCGAC
    AGAATAATTG GAGCGCGGCG AGTTGGCGGA GGACGTGCTC TGGGGCACAC CATCAGCGAG
    GACGAGGATG ACAATGGTTT GAACTCGCTG GCCCTCGAGG ACGAAAACAG TCCCACTGTG
    CGGGTCAAAT TGTGTCTGAG GACCAATCCA GAGCACTGCG GCGATTACAC GGACGCAGAA
    ATTGGCAGGC CGTATATAGC GGAGGCTAAT GCCCTGGCCA CGCCCACTCT CATTGCGATA
    ATCGTGTCCT GCGTGGTCTT TGCCCTCTTC GCTGGCCTAA TCCTGATGTT CTGCCGCTGC
    AAGCGCAATC AATCGAAGAA GAGCGCCGCC GCCAAAGATT ACGAGATGGA CTCGGTGCGT
    CCTTCGATTG TGGCCGCTCA GCAAAACCAG GCGCCACCAC CCTACTACCC CGCCTCTGGG
    CTGGACAACA AGGCATTGGA ACACTCAATG GATCTCGCCC TCAGCATGGA GGATCAGAAA
    ACGGCACTAT ACGCCACCCA GAATGGATAT AGCTATCATC CGGGAAGTGG GGTGGTGGGC
    GTTGGCATGG GCGGCGGCGT TGTGGGAGTG GGTGTTGGCG GCAGCGTGGT CAGCGGAATG
    GGCGGCGGAG TGGGCGGAAT CGGAGGCAGT GGCGTCGGCG TCAATGGAAT ACCTGGACTC
    AGCGCACACA CGATGCCCGG AAATGAATGG GTCAACATGG GCTACATGGA GAACAACTAT
    TCGAATTCGA ACAACGGCGG CAGTGTCAAC TCGCAGGACT CACTGTGGCA GGTGAAGATG
    TCAGCCGCGG CGGTGGGCAA CCAGCAGGGC ATGGTGCAGG CCCCAATGAA TCAGTATGTG
    GAACAGCAGC CCGCCTACGG ATACGATCCG TTGACCCATG GTGGCTATGG GGCGGTGGAC
    GACTACGCTC CCTATCCGCA CCTCACGGCC ACGCCCAGCC AGGTGGGCGA TGAGTACCAC
    AACTTGCGCA ACAGCCAGAA TCCGTCCCGG CAGGACTACT GCAGCGATCC CTATGCATCC
    GTGCAAAAGC CCAAAAAGCG AGTCGATCAG CATTTAGCGG ACATTATACA GGATGTTACG
    CTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260014.1
    CDS433..4278
    Translation

    Target ORF information:

    RefSeq Version NM_001273085.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster echinoid (ed), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273085.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGGCGAA AAACTACCAC GAAAGGGACG GCAATAGTCA ATAGCAGGAG TGCGAGGAGA 
    GCAGCAACGA CTATATGCAA CTTTTTTCTC ATCGCACTCG TAGCTCTGAG TTCCACGACT
    CTGGCCGATG AATCCATTGA CACACGTGAG AATGCTGACT TAACCCTCAA GTGCCGGTTT
    AACGACAAGT ATGAGGCGAA CGATTTCTCT TTCTTCTGGA CCCGCTGGAC AGCGAATCCC
    GCCCAATTCG ATAACGTAGC CATCGGTGAA GTGCAGTTGA GTTCCGGCTA TAGACTCGAC
    TTTCAACCGG AGCGTGGCAT TTACGATCTG CAGATCAAGA ATGTGTCCTA TAACCGTGAC
    AATGGACGCT TCGAGTGCCG CATCAAGGCC AAGGGAACGG GCGCAGACGT CCATCAGGAG
    TTCCACAATC TCACGGTGCT CACGCCACCT CATCCACCCG TGATCTCACC CGGAAACATA
    GCCGTGGCCA CCGAGGACAA GCCCATGGAG CTGACATGCA GCAGCATCGG TGGTTCCCCC
    GATCCCACCA TAACTTGGTA TCGCGAGGGT TCGAATACGC CCCTGCCAGC GACGGTGTTA
    AAGGGCGGTA CCAAGGATCA GCCCACCAAT GCCACGCTAT CCATAATTCC TCGCAGAGAA
    GATGACGGTG CCAAGTACAA GTGTGTAGTC AGAAATCGTG CCATGAACGA GGGAAAGCGA
    TTGGAGGCCA CTGCCACATT GAATGTTAAC TATTACCCCC GAGTCGAGGT TGGACCCGAG
    AATCCTTTGC GGGTGGAACG TGACCGGACT GCCAAACTGG AGTGTAATGT GGATGCCAAG
    CCGAAGGTTC CCAATGTCCG TTGGAATCGC AATGGTCGGT TCATCAGCTC CTCATTAGTT
    CACACGATCC ATCGGGTGAG TGTTCAAGAT GCCGGCAAGT ATACCTGCAT TGCGGACAAC
    GGTCTTGGAA AGACCGGAGA ACAGGAGCTC ATCCTGGATA TCCTGTATCC ACCCATGGTC
    GTGATAGAGT CCAAGACGCG AGAGGCCGAA GAGGGCGATA CAGTGACCAT TCGCTGCAAT
    GTCACAGCCA ATCCTGCACC CGTGACCATT GAGTGGCTCA AGGAGAACAG TCCCGACTTC
    CGCTACAATG GCGACGTACT GACCCTAACT TCCGTGCGAG CCGATCATGC CGGAAACTAC
    ATCTGCCGTG CCGTGAACAT AATGCAGAGC CAGGGAATGG AGCGCAGTGA ACGAGTGGGT
    AACTCCACGG TGGCCCTGCT AGTTCGCCAT CGACCAGGAC AGGCTTACAT TACACCCAAT
    AAGCCCGTGG TTCATGTGGG TAATGGTGTC ACCTTGACCT GCTCCGCTAA TCCGCCAGGC
    TGGCCAGTGC CCCAGTACAG ATGGTTCAGG GATATGGATG GAGAGTTCTC CAGCACGCAG
    AAAATCCTTG CACAAGGCCC TCAGTACTCG ATACCCAAAG CTCACTTGGG TAACGAGGGT
    AAATACCATT GTCATGCTGT AAATGAACTG GGCATCGGAA TGATGGCCAC CATTGTCCTG
    GAGATCCACC AGCCTCCGCA GTTCTTGGCC AAGCTACAGC AGCATATGAC TCGCAGAGTG
    GCCGATACGG ATTATACGGT CACGTGCAGT GCCAAGGGAA AGCCAGCACC CAGTGTTAAA
    TGGCTGAAGG ACGCAGTGGA AATCCTGCCC GAGGAAAATC TCTACGAAGT GCAGACCAAT
    CCCGATCAGG GCCTCAACGG CATGGTCACG GTTCAGAGTC AACTTAAGTT CCGAGGAAAG
    GCCAGACCGA ATGGAAACGC TTTAGTGCCT GGAGATCGTG GCTTGTACAC CTGCTTGTAT
    CAGAACGAGG TCAACTCGGC CAACTCGTCC ATGCAACTGA GGATCGAACA TGAGCCCATT
    GTACTCCATC AGTATAACAA GGTGGCCTTT GATATCAGGG AGACAGCCGA GGTGGTTTGC
    AAGGTTCAGG CCTATCCCAA GCCCGAGTTC CAATGGCAGT TTGGCAATAA TCCATCGCCC
    CTGACCATGT CCTCCGATGG TCACTACGAG ATTAGTACCA CCACCGATAA CAACGACATC
    TATACGTCCG TGCTCAAGAT CAACTCGCTG ACTCATTCGG ATTACGGAGA ATACACCTGC
    CGGGTGGCCA ACACCCTGGA TACGATTCGA GCTCCCATTA GACTGCAGCA GAAAGGACCC
    CCAGAGAAAC CAACAAATCT GCGAGCCACC GAGGTGGGTC ACAATTACGT ATCACTGAGC
    TGGGATCCCG GATTCGATGG AGGTCTGAGC AAGACCAAGT TCTTTGTTAG CTATCGCCGT
    GTGGCCATGC CAAGGGAAGA ACAATTGATA CCGGATTGCG CCACTTTGGC CAACTCGAAT
    TCGGCTTGGG TGGAAGTCGA TTGCCAGCGG GATATTCCCT GCAAGGTGAC AGCCCTGGAG
    CAACACCAGA GTTATGCCTT TAAGGTCAAG GCTCTGAATC CCAAGAGCGA TTCACCGTAC
    TCGAGTGAAA TCATGGTGAC GACAAAGGTC AGCAGGATTC CGCCACCATT GCAGGTGACC
    TATGAGCCGA ATACTCGAAC TCTCGGCATC GATGTGGGGG CCACGTGCTT GAACCTGGTG
    GCCGTTGTGG AGTCCATGGT GAATGCAGAT TCTCCGATGG CCGCCTGGGA GGTGGTGACC
    ACCATGGATA ACCTGCAGCT GTCCGGAAAT GGACCCACCC ACAAGGAGAA GATCATCGAC
    AGAATAATTG GAGCGCGGCG AGTTGGCGGA GGACGTGCTC TGGGGCACAC CATCAGCGAG
    GACGAGGATG ACAATGGTTT GAACTCGCTG GCCCTCGAGG ACGAAAACAG TCCCACTGTG
    CGGGTCAAAT TGTGTCTGAG GACCAATCCA GAGCACTGCG GCGATTACAC GGACGCAGAA
    ATTGGCAGGC CGTATATAGC GGAGGCTAAT GCCCTGGCCA CGCCCACTCT CATTGCGATA
    ATCGTGTCCT GCGTGGTCTT TGCCCTCTTC GCTGGCCTAA TCCTGATGTT CTGCCGCTGC
    AAGCGCAATC AATCGAAGAA GAGCGCCGCC GCCAAAGATT ACGAGATGGA CTCGGTGCGT
    CCTTCGATTG TGGCCGCTCA GCAAAACCAG GCGCCACCAC CCTACTACCC CGCCTCTGGG
    CTGGACAACA AGGCATTGGA ACACTCAATG GATCTCGCCC TCAGCATGGA GGATCAGAAA
    ACGGCACTAT ACGCCACCCA GAATGGATAT AGCTATCATC CGGGAAGTGG GGTGGTGGGC
    GTTGGCATGG GCGGCGGCGT TGTGGGAGTG GGTGTTGGCG GCAGCGTGGT CAGCGGAATG
    GGCGGCGGAG TGGGCGGAAT CGGAGGCAGT GGCGTCGGCG TCAATGGAAT ACCTGGACTC
    AGCGCACACA CGATGCCCGG AAATGAATGG GTCAACATGG GCTACATGGA GAACAACTAT
    TCGAATTCGA ACAACGGCGG CAGTGTCAAC TCGCAGGACT CACTGTGGCA GGTGAAGATG
    TCAGCCGCGG CGGTGGGCAA CCAGCAGGGC ATGGTGCAGG CCCCAATGAA TCAGTATGTG
    GAACAGCAGC CCGCCTACGG ATACGATCCG TTGACCCATG GTGGCTATGG GGCGGTGGAC
    GACTACGCTC CCTATCCGCA CCTCACGGCC ACGCCCAGCC AGGTGGGCGA TGAGTACCAC
    AACTTGCGCA ACAGCCAGAA TCCGTCCCGG CAGGACTACT GCAGCGATCC CTATGCATCC
    GTGCAAAAGC CCAAAAAGCG AGTCGATCAG CATTTAGCGG ACATTATACA GGATGTTACG
    CTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.