ed cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ed
Entrez Gene ID	33619
Full Name	echinoid
Synonyms	1X5,CG12676,CG15424,CG16842,CT13476,CT35484,Dmel\CG12676,E(Elp)24D,E(Elp)[24D],Ed,l(2)k01102
General protein information	Preferred Names echinoid Names CG12676 gene product from transcript CG12676-RB CG12676-PA CG12676-PB CG12676-PC ed-PA ed-PB ed-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 24D4-24D6\|2-11.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_078745.3	NP_523469.2	echinoid, isoform A
NM_001273084.1	NP_001260013.1	echinoid, isoform B
NM_001273085.1	NP_001260014.1	echinoid, isoform C

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	igcm-1	NP_508166.1
Drosophila melanogaster (fruit fly)	ed	NP_001260013.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010186	XP_319363.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Together, our results demonstrate that transcriptional repression of Ed followed by Smurf-mediated downregulation of pretranslated Ed in amnioserosa regulates the establishment of a taut leading edge during dorsal closure
Title: Smurf Downregulates Echinoid in the Amnioserosa To Regulate

Data propose that Ed-mediated cell sorting is driven both by differential Ed adhesion that induces cell segregation with a jagged border and by actomyosin cable assembly at the interface that smoothens this border.
Title: Differential adhesion and actomyosin cable collaborate to drive Echinoid-mediated cell sorting.

The planar polarized distribution of Echinoid in the DME cells thus provides a spatial cue that polarizes the DME cell actin cytoskeleton, defining the epidermal leading edge and establishing its contractile properties.
Title: Asymmetric distribution of Echinoid defines the epidermal leading edge during Drosophila dorsal closure.

Data show that tracheal-specific expression of Echinoid rescues cell fusion defects, indicating that Ed acts in the trachea to control fusion cell fate.
Title: Echinoid regulates tracheal morphology and fusion cell fate in Drosophila.

Co-endocytosis of Ed and Fmi provides a link between apicobasal polarity and planar cell polarity.
Title: Echinoid regulates Flamingo endocytosis to control ommatidial rotation in the Drosophila eye.

The following ed gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ed cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13703	NM_078745.3 Latest version!	Drosophila melanogaster echinoid (ed), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa13703	NM_001273084.1 Latest version!	Drosophila melanogaster echinoid (ed), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa16200	NM_001273085.1 Latest version!	Drosophila melanogaster echinoid (ed), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ed ( NM_078745.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523469.2 Drosophila melanogaster echinoid (ed), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13703
Clone ID Related Accession (Same CDS sequence)	NM_078745.3 , NM_001273084.1
Accession Version	NM_078745.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	echinoid, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_078745.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGGCGAA AAACTACCAC GAAAGGGACG GCAATAGTCA ATAGCAGGAG TGCGAGGAGA 
GCAGCAACGA CTATATGCAA CTTTTTTCTC ATCGCACTCG TAGCTCTGAG TTCCACGACT 
CTGGCCGATG AATCCATTGA CACACGTGAG AATGCTGACT TAACCCTCAA GTGCCGGTTT 
AACGACAAGT ATGAGGCGAA CGATTTCTCT TTCTTCTGGA CCCGCTGGAC AGCGAATCCC 
GCCCAATTCG ATAACGTAGC CATCGGTGAA GTGCAGTTGA GTTCCGGCTA TAGACTCGAC 
TTTCAACCGG AGCGTGGCAT TTACGATCTG CAGATCAAGA ATGTGTCCTA TAACCGTGAC 
AATGGACGCT TCGAGTGCCG CATCAAGGCC AAGGGAACGG GCGCAGACGT CCATCAGGAG 
TTCCACAATC TCACGGTGCT CACGCCACCT CATCCACCCG TGATCTCACC CGGAAACATA 
GCCGTGGCCA CCGAGGACAA GCCCATGGAG CTGACATGCA GCAGCATCGG TGGTTCCCCC 
GATCCCACCA TAACTTGGTA TCGCGAGGGT TCGAATACGC CCCTGCCAGC GACGGTGTTA 
AAGGGCGGTA CCAAGGATCA GCCCACCAAT GCCACGCTAT CCATAATTCC TCGCAGAGAA 
GATGACGGTG CCAAGTACAA GTGTGTAGTC AGAAATCGTG CCATGAACGA GGGAAAGCGA 
TTGGAGGCCA CTGCCACATT GAATGTTAAC TATTACCCCC GAGTCGAGGT TGGACCCGAG 
AATCCTTTGC GGGTGGAACG TGACCGGACT GCCAAACTGG AGTGTAATGT GGATGCCAAG 
CCGAAGGTTC CCAATGTCCG TTGGAATCGC AATGGTCGGT TCATCAGCTC CTCATTAGTT 
CACACGATCC ATCGGGTGAG TGTTCAAGAT GCCGGCAAGT ATACCTGCAT TGCGGACAAC 
GGTCTTGGAA AGACCGGAGA ACAGGAGCTC ATCCTGGATA TCCTGTATCC ACCCATGGTC 
GTGATAGAGT CCAAGACGCG AGAGGCCGAA GAGGGCGATA CAGTGACCAT TCGCTGCAAT 
GTCACAGCCA ATCCTGCACC CGTGACCATT GAGTGGCTCA AGGAGAACAG TCCCGACTTC 
CGCTACAATG GCGACGTACT GACCCTAACT TCCGTGCGAG CCGATCATGC CGGAAACTAC 
ATCTGCCGTG CCGTGAACAT AATGCAGAGC CAGGGAATGG AGCGCAGTGA ACGAGTGGGT 
AACTCCACGG TGGCCCTGCT AGTTCGCCAT CGACCAGGAC AGGCTTACAT TACACCCAAT 
AAGCCCGTGG TTCATGTGGG TAATGGTGTC ACCTTGACCT GCTCCGCTAA TCCGCCAGGC 
TGGCCAGTGC CCCAGTACAG ATGGTTCAGG GATATGGATG GAGAGTTCTC CAGCACGCAG 
AAAATCCTTG CACAAGGCCC TCAGTACTCG ATACCCAAAG CTCACTTGGG TAACGAGGGT 
AAATACCATT GTCATGCTGT AAATGAACTG GGCATCGGAA TGATGGCCAC CATTGTCCTG 
GAGATCCACC AGCCTCCGCA GTTCTTGGCC AAGCTACAGC AGCATATGAC TCGCAGAGTG 
GCCGATACGG ATTATACGGT CACGTGCAGT GCCAAGGGAA AGCCAGCACC CAGTGTTAAA 
TGGCTGAAGG ACGCAGTGGA AATCCTGCCC GAGGAAAATC TCTACGAAGT GCAGACCAAT 
CCCGATCAGG GCCTCAACGG CATGGTCACG GTTCAGAGTC AACTTAAGTT CCGAGGAAAG 
GCCAGACCGA ATGGAAACGC TTTAGTGCCT GGAGATCGTG GCTTGTACAC CTGCTTGTAT 
CAGAACGAGG TCAACTCGGC CAACTCGTCC ATGCAACTGA GGATCGAACA TGAGCCCATT 
GTACTCCATC AGTATAACAA GGTGGCCTTT GATATCAGGG AGACAGCCGA GGTGGTTTGC 
AAGGTTCAGG CCTATCCCAA GCCCGAGTTC CAATGGCAGT TTGGCAATAA TCCATCGCCC 
CTGACCATGT CCTCCGATGG TCACTACGAG ATTAGTACCA CCACCGATAA CAACGACATC 
TATACGTCCG TGCTCAAGAT CAACTCGCTG ACTCATTCGG ATTACGGAGA ATACACCTGC 
CGGGTGGCCA ACACCCTGGA TACGATTCGA GCTCCCATTA GACTGCAGCA GAAAGGACCC 
CCAGAGAAAC CAACAAATCT GCGAGCCACC GAGGTGGGTC ACAATTACGT ATCACTGAGC 
TGGGATCCCG GATTCGATGG AGGTCTGAGC AAGACCAAGT TCTTTGTTAG CTATCGCCGT 
GTGGCCATGC CAAGGGAAGA ACAATTGATA CCGGATTGCG CCACTTTGGC CAACTCGAAT 
TCGGCTTGGG TGGAAGTCGA TTGCCAGCGG GATATTCCCT GCAAGGTGAC AGCCCTGGAG 
CAACACCAGA GTTATGCCTT TAAGGTCAAG GCTCTGAATC CCAAGAGCGA TTCACCGTAC 
TCGAGTGAAA TCATGGTGAC GACAAAGGTC AGCAGGATTC CGCCACCATT GCAGGTGACC 
TATGAGCCGA ATACTCGAAC TCTCGGCATC GATGTGGGGG CCACGTGCTT GAACCTGGTG 
GCCGTTGTGG AGTCCATGGT GAATGCAGAT TCTCCGATGG CCGCCTGGGA GGTGGTGACC 
ACCATGGATA ACCTGCAGCT GTCCGGAAAT GGACCCACCC ACAAGGAGAA GATCATCGAC 
AGAATAATTG GAGCGCGGCG AGTTGGCGGA GGACGTGCTC TGGGGCACAC CATCAGCGAG 
GACGAGGATG ACAATGGTTT GAACTCGCTG GCCCTCGAGG ACGAAAACAG TCCCACTGTG 
CGGGTCAAAT TGTGTCTGAG GACCAATCCA GAGCACTGCG GCGATTACAC GGACGCAGAA 
ATTGGCAGGC CGTATATAGC GGAGGCTAAT GCCCTGGCCA CGCCCACTCT CATTGCGATA 
ATCGTGTCCT GCGTGGTCTT TGCCCTCTTC GCTGGCCTAA TCCTGATGTT CTGCCGCTGC 
AAGCGCAATC AATCGAAGAA GAGCGCCGCC GCCAAAGATT ACGAGATGGA CTCGGTGCGT 
CCTTCGATTG TGGCCGCTCA GCAAAACCAG GCGCCACCAC CCTACTACCC CGCCTCTGGG 
CTGGACAACA AGGCATTGGA ACACTCAATG GATCTCGCCC TCAGCATGGA GGATCAGAAA 
ACGGCACTAT ACGCCACCCA GAATGGATAT AGCTATCATC CGGGAAGTGG GGTGGTGGGC 
GTTGGCATGG GCGGCGGCGT TGTGGGAGTG GGTGTTGGCG GCAGCGTGGT CAGCGGAATG 
GGCGGCGGAG TGGGCGGAAT CGGAGGCAGT GGCGTCGGCG TCAATGGAAT ACCTGGACTC 
AGCGCACACA CGATGCCCGG AAATGAATGG GTCAACATGG GCTACATGGA GAACAACTAT 
TCGAATTCGA ACAACGGCGG CAGTGTCAAC TCGCAGGACT CACTGTGGCA GGTGAAGATG 
TCAGCCGCGG CGGTGGGCAA CCAGCAGGGC ATGGTGCAGG CCCCAATGAA TCAGTATGTG 
GAACAGCAGC CCGCCTACGG ATACGATCCG TTGACCCATG GTGGCTATGG GGCGGTGGAC 
GACTACGCTC CCTATCCGCA CCTCACGGCC ACGCCCAGCC AGGTGGGCGA TGAGTACCAC 
AACTTGCGCA ACAGCCAGAA TCCGTCCCGG CAGGACTACT GCAGCGATCC CTATGCATCC 
GTGCAAAAGC CCAAAAAGCG AGTCGATCAG CATTTAGATT CACCATATCA CGACGTAAGC 
GGTCTGCCCA ATCCCTACAA CATGGAGCAT TTGGAACAGG ACGAGGTCCT TCCCCCGCAG 
CAGCACATGT CCTTGAGCTA CGACGACAGC TTCGAGGGCG AATATTCGAC AACGCCGAAT 
GCCAGAAACC GTCGCGTCAT ACGCGAGATT ATTGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523469.2
CDS	433..4431
Translation	MRRKTTTKGTAIVNSRSARRAATTICNFFLIALVALSSTTLADESIDTRENADLTLKCRFNDKYEANDFSFFWTRWTANPAQFDNVAIGEVQLSSGYRLDFQPERGIYDLQIKNVSYNRDNGRFECRIKAKGTGADVHQEFHNLTVLTPPHPPVISPGNIAVATEDKPMELTCSSIGGSPDPTITWYREGSNTPLPATVLKGGTKDQPTNATLSIIPRREDDGAKYKCVVRNRAMNEGKRLEATATLNVNYYPRVEVGPENPLRVERDRTAKLECNVDAKPKVPNVRWNRNGRFISSSLVHTIHRVSVQDAGKYTCIADNGLGKTGEQELILDILYPPMVVIESKTREAEEGDTVTIRCNVTANPAPVTIEWLKENSPDFRYNGDVLTLTSVRADHAGNYICRAVNIMQSQGMERSERVGNSTVALLVRHRPGQAYITPNKPVVHVGNGVTLTCSANPPGWPVPQYRWFRDMDGEFSSTQKILAQGPQYSIPKAHLGNEGKYHCHAVNELGIGMMATIVLEIHQPPQFLAKLQQHMTRRVADTDYTVTCSAKGKPAPSVKWLKDAVEILPEENLYEVQTNPDQGLNGMVTVQSQLKFRGKARPNGNALVPGDRGLYTCLYQNEVNSANSSMQLRIEHEPIVLHQYNKVAFDIRETAEVVCKVQAYPKPEFQWQFGNNPSPLTMSSDGHYEISTTTDNNDIYTSVLKINSLTHSDYGEYTCRVANTLDTIRAPIRLQQKGPPEKPTNLRATEVGHNYVSLSWDPGFDGGLSKTKFFVSYRRVAMPREEQLIPDCATLANSNSAWVEVDCQRDIPCKVTALEQHQSYAFKVKALNPKSDSPYSSEIMVTTKVSRIPPPLQVTYEPNTRTLGIDVGATCLNLVAVVESMVNADSPMAAWEVVTTMDNLQLSGNGPTHKEKIIDRIIGARRVGGGRALGHTISEDEDDNGLNSLALEDENSPTVRVKLCLRTNPEHCGDYTDAEIGRPYIAEANALATPTLIAIIVSCVVFALFAGLILMFCRCKRNQSKKSAAAKDYEMDSVRPSIVAAQQNQAPPPYYPASGLDNKALEHSMDLALSMEDQKTALYATQNGYSYHPGSGVVGVGMGGGVVGVGVGGSVVSGMGGGVGGIGGSGVGVNGIPGLSAHTMPGNEWVNMGYMENNYSNSNNGGSVNSQDSLWQVKMSAAAVGNQQGMVQAPMNQYVEQQPAYGYDPLTHGGYGAVDDYAPYPHLTATPSQVGDEYHNLRNSQNPSRQDYCSDPYASVQKPKKRVDQHLDSPYHDVSGLPNPYNMEHLEQDEVLPPQQHMSLSYDDSFEGEYSTTPNARNRRVIREIIV

Target ORF information:

RefSeq Version	NM_078745.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster echinoid (ed), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078745.3

ORF Insert Sequence:

ATGAGGCGAA AAACTACCAC GAAAGGGACG GCAATAGTCA ATAGCAGGAG TGCGAGGAGA 
GCAGCAACGA CTATATGCAA CTTTTTTCTC ATCGCACTCG TAGCTCTGAG TTCCACGACT 
CTGGCCGATG AATCCATTGA CACACGTGAG AATGCTGACT TAACCCTCAA GTGCCGGTTT 
AACGACAAGT ATGAGGCGAA CGATTTCTCT TTCTTCTGGA CCCGCTGGAC AGCGAATCCC 
GCCCAATTCG ATAACGTAGC CATCGGTGAA GTGCAGTTGA GTTCCGGCTA TAGACTCGAC 
TTTCAACCGG AGCGTGGCAT TTACGATCTG CAGATCAAGA ATGTGTCCTA TAACCGTGAC 
AATGGACGCT TCGAGTGCCG CATCAAGGCC AAGGGAACGG GCGCAGACGT CCATCAGGAG 
TTCCACAATC TCACGGTGCT CACGCCACCT CATCCACCCG TGATCTCACC CGGAAACATA 
GCCGTGGCCA CCGAGGACAA GCCCATGGAG CTGACATGCA GCAGCATCGG TGGTTCCCCC 
GATCCCACCA TAACTTGGTA TCGCGAGGGT TCGAATACGC CCCTGCCAGC GACGGTGTTA 
AAGGGCGGTA CCAAGGATCA GCCCACCAAT GCCACGCTAT CCATAATTCC TCGCAGAGAA 
GATGACGGTG CCAAGTACAA GTGTGTAGTC AGAAATCGTG CCATGAACGA GGGAAAGCGA 
TTGGAGGCCA CTGCCACATT GAATGTTAAC TATTACCCCC GAGTCGAGGT TGGACCCGAG 
AATCCTTTGC GGGTGGAACG TGACCGGACT GCCAAACTGG AGTGTAATGT GGATGCCAAG 
CCGAAGGTTC CCAATGTCCG TTGGAATCGC AATGGTCGGT TCATCAGCTC CTCATTAGTT 
CACACGATCC ATCGGGTGAG TGTTCAAGAT GCCGGCAAGT ATACCTGCAT TGCGGACAAC 
GGTCTTGGAA AGACCGGAGA ACAGGAGCTC ATCCTGGATA TCCTGTATCC ACCCATGGTC 
GTGATAGAGT CCAAGACGCG AGAGGCCGAA GAGGGCGATA CAGTGACCAT TCGCTGCAAT 
GTCACAGCCA ATCCTGCACC CGTGACCATT GAGTGGCTCA AGGAGAACAG TCCCGACTTC 
CGCTACAATG GCGACGTACT GACCCTAACT TCCGTGCGAG CCGATCATGC CGGAAACTAC 
ATCTGCCGTG CCGTGAACAT AATGCAGAGC CAGGGAATGG AGCGCAGTGA ACGAGTGGGT 
AACTCCACGG TGGCCCTGCT AGTTCGCCAT CGACCAGGAC AGGCTTACAT TACACCCAAT 
AAGCCCGTGG TTCATGTGGG TAATGGTGTC ACCTTGACCT GCTCCGCTAA TCCGCCAGGC 
TGGCCAGTGC CCCAGTACAG ATGGTTCAGG GATATGGATG GAGAGTTCTC CAGCACGCAG 
AAAATCCTTG CACAAGGCCC TCAGTACTCG ATACCCAAAG CTCACTTGGG TAACGAGGGT 
AAATACCATT GTCATGCTGT AAATGAACTG GGCATCGGAA TGATGGCCAC CATTGTCCTG 
GAGATCCACC AGCCTCCGCA GTTCTTGGCC AAGCTACAGC AGCATATGAC TCGCAGAGTG 
GCCGATACGG ATTATACGGT CACGTGCAGT GCCAAGGGAA AGCCAGCACC CAGTGTTAAA 
TGGCTGAAGG ACGCAGTGGA AATCCTGCCC GAGGAAAATC TCTACGAAGT GCAGACCAAT 
CCCGATCAGG GCCTCAACGG CATGGTCACG GTTCAGAGTC AACTTAAGTT CCGAGGAAAG 
GCCAGACCGA ATGGAAACGC TTTAGTGCCT GGAGATCGTG GCTTGTACAC CTGCTTGTAT 
CAGAACGAGG TCAACTCGGC CAACTCGTCC ATGCAACTGA GGATCGAACA TGAGCCCATT 
GTACTCCATC AGTATAACAA GGTGGCCTTT GATATCAGGG AGACAGCCGA GGTGGTTTGC 
AAGGTTCAGG CCTATCCCAA GCCCGAGTTC CAATGGCAGT TTGGCAATAA TCCATCGCCC 
CTGACCATGT CCTCCGATGG TCACTACGAG ATTAGTACCA CCACCGATAA CAACGACATC 
TATACGTCCG TGCTCAAGAT CAACTCGCTG ACTCATTCGG ATTACGGAGA ATACACCTGC 
CGGGTGGCCA ACACCCTGGA TACGATTCGA GCTCCCATTA GACTGCAGCA GAAAGGACCC 
CCAGAGAAAC CAACAAATCT GCGAGCCACC GAGGTGGGTC ACAATTACGT ATCACTGAGC 
TGGGATCCCG GATTCGATGG AGGTCTGAGC AAGACCAAGT TCTTTGTTAG CTATCGCCGT 
GTGGCCATGC CAAGGGAAGA ACAATTGATA CCGGATTGCG CCACTTTGGC CAACTCGAAT 
TCGGCTTGGG TGGAAGTCGA TTGCCAGCGG GATATTCCCT GCAAGGTGAC AGCCCTGGAG 
CAACACCAGA GTTATGCCTT TAAGGTCAAG GCTCTGAATC CCAAGAGCGA TTCACCGTAC 
TCGAGTGAAA TCATGGTGAC GACAAAGGTC AGCAGGATTC CGCCACCATT GCAGGTGACC 
TATGAGCCGA ATACTCGAAC TCTCGGCATC GATGTGGGGG CCACGTGCTT GAACCTGGTG 
GCCGTTGTGG AGTCCATGGT GAATGCAGAT TCTCCGATGG CCGCCTGGGA GGTGGTGACC 
ACCATGGATA ACCTGCAGCT GTCCGGAAAT GGACCCACCC ACAAGGAGAA GATCATCGAC 
AGAATAATTG GAGCGCGGCG AGTTGGCGGA GGACGTGCTC TGGGGCACAC CATCAGCGAG 
GACGAGGATG ACAATGGTTT GAACTCGCTG GCCCTCGAGG ACGAAAACAG TCCCACTGTG 
CGGGTCAAAT TGTGTCTGAG GACCAATCCA GAGCACTGCG GCGATTACAC GGACGCAGAA 
ATTGGCAGGC CGTATATAGC GGAGGCTAAT GCCCTGGCCA CGCCCACTCT CATTGCGATA 
ATCGTGTCCT GCGTGGTCTT TGCCCTCTTC GCTGGCCTAA TCCTGATGTT CTGCCGCTGC 
AAGCGCAATC AATCGAAGAA GAGCGCCGCC GCCAAAGATT ACGAGATGGA CTCGGTGCGT 
CCTTCGATTG TGGCCGCTCA GCAAAACCAG GCGCCACCAC CCTACTACCC CGCCTCTGGG 
CTGGACAACA AGGCATTGGA ACACTCAATG GATCTCGCCC TCAGCATGGA GGATCAGAAA 
ACGGCACTAT ACGCCACCCA GAATGGATAT AGCTATCATC CGGGAAGTGG GGTGGTGGGC 
GTTGGCATGG GCGGCGGCGT TGTGGGAGTG GGTGTTGGCG GCAGCGTGGT CAGCGGAATG 
GGCGGCGGAG TGGGCGGAAT CGGAGGCAGT GGCGTCGGCG TCAATGGAAT ACCTGGACTC 
AGCGCACACA CGATGCCCGG AAATGAATGG GTCAACATGG GCTACATGGA GAACAACTAT 
TCGAATTCGA ACAACGGCGG CAGTGTCAAC TCGCAGGACT CACTGTGGCA GGTGAAGATG 
TCAGCCGCGG CGGTGGGCAA CCAGCAGGGC ATGGTGCAGG CCCCAATGAA TCAGTATGTG 
GAACAGCAGC CCGCCTACGG ATACGATCCG TTGACCCATG GTGGCTATGG GGCGGTGGAC 
GACTACGCTC CCTATCCGCA CCTCACGGCC ACGCCCAGCC AGGTGGGCGA TGAGTACCAC 
AACTTGCGCA ACAGCCAGAA TCCGTCCCGG CAGGACTACT GCAGCGATCC CTATGCATCC 
GTGCAAAAGC CCAAAAAGCG AGTCGATCAG CATTTAGATT CACCATATCA CGACGTAAGC 
GGTCTGCCCA ATCCCTACAA CATGGAGCAT TTGGAACAGG ACGAGGTCCT TCCCCCGCAG 
CAGCACATGT CCTTGAGCTA CGACGACAGC TTCGAGGGCG AATATTCGAC AACGCCGAAT 
GCCAGAAACC GTCGCGTCAT ACGCGAGATT ATTGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ed ( NM_001273084.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260013.1 Drosophila melanogaster echinoid (ed), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13703
Clone ID Related Accession (Same CDS sequence)	NM_078745.3 , NM_001273084.1
Accession Version	NM_001273084.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	echinoid, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGGCGAA AAACTACCAC GAAAGGGACG GCAATAGTCA ATAGCAGGAG TGCGAGGAGA 
GCAGCAACGA CTATATGCAA CTTTTTTCTC ATCGCACTCG TAGCTCTGAG TTCCACGACT 
CTGGCCGATG AATCCATTGA CACACGTGAG AATGCTGACT TAACCCTCAA GTGCCGGTTT 
AACGACAAGT ATGAGGCGAA CGATTTCTCT TTCTTCTGGA CCCGCTGGAC AGCGAATCCC 
GCCCAATTCG ATAACGTAGC CATCGGTGAA GTGCAGTTGA GTTCCGGCTA TAGACTCGAC 
TTTCAACCGG AGCGTGGCAT TTACGATCTG CAGATCAAGA ATGTGTCCTA TAACCGTGAC 
AATGGACGCT TCGAGTGCCG CATCAAGGCC AAGGGAACGG GCGCAGACGT CCATCAGGAG 
TTCCACAATC TCACGGTGCT CACGCCACCT CATCCACCCG TGATCTCACC CGGAAACATA 
GCCGTGGCCA CCGAGGACAA GCCCATGGAG CTGACATGCA GCAGCATCGG TGGTTCCCCC 
GATCCCACCA TAACTTGGTA TCGCGAGGGT TCGAATACGC CCCTGCCAGC GACGGTGTTA 
AAGGGCGGTA CCAAGGATCA GCCCACCAAT GCCACGCTAT CCATAATTCC TCGCAGAGAA 
GATGACGGTG CCAAGTACAA GTGTGTAGTC AGAAATCGTG CCATGAACGA GGGAAAGCGA 
TTGGAGGCCA CTGCCACATT GAATGTTAAC TATTACCCCC GAGTCGAGGT TGGACCCGAG 
AATCCTTTGC GGGTGGAACG TGACCGGACT GCCAAACTGG AGTGTAATGT GGATGCCAAG 
CCGAAGGTTC CCAATGTCCG TTGGAATCGC AATGGTCGGT TCATCAGCTC CTCATTAGTT 
CACACGATCC ATCGGGTGAG TGTTCAAGAT GCCGGCAAGT ATACCTGCAT TGCGGACAAC 
GGTCTTGGAA AGACCGGAGA ACAGGAGCTC ATCCTGGATA TCCTGTATCC ACCCATGGTC 
GTGATAGAGT CCAAGACGCG AGAGGCCGAA GAGGGCGATA CAGTGACCAT TCGCTGCAAT 
GTCACAGCCA ATCCTGCACC CGTGACCATT GAGTGGCTCA AGGAGAACAG TCCCGACTTC 
CGCTACAATG GCGACGTACT GACCCTAACT TCCGTGCGAG CCGATCATGC CGGAAACTAC 
ATCTGCCGTG CCGTGAACAT AATGCAGAGC CAGGGAATGG AGCGCAGTGA ACGAGTGGGT 
AACTCCACGG TGGCCCTGCT AGTTCGCCAT CGACCAGGAC AGGCTTACAT TACACCCAAT 
AAGCCCGTGG TTCATGTGGG TAATGGTGTC ACCTTGACCT GCTCCGCTAA TCCGCCAGGC 
TGGCCAGTGC CCCAGTACAG ATGGTTCAGG GATATGGATG GAGAGTTCTC CAGCACGCAG 
AAAATCCTTG CACAAGGCCC TCAGTACTCG ATACCCAAAG CTCACTTGGG TAACGAGGGT 
AAATACCATT GTCATGCTGT AAATGAACTG GGCATCGGAA TGATGGCCAC CATTGTCCTG 
GAGATCCACC AGCCTCCGCA GTTCTTGGCC AAGCTACAGC AGCATATGAC TCGCAGAGTG 
GCCGATACGG ATTATACGGT CACGTGCAGT GCCAAGGGAA AGCCAGCACC CAGTGTTAAA 
TGGCTGAAGG ACGCAGTGGA AATCCTGCCC GAGGAAAATC TCTACGAAGT GCAGACCAAT 
CCCGATCAGG GCCTCAACGG CATGGTCACG GTTCAGAGTC AACTTAAGTT CCGAGGAAAG 
GCCAGACCGA ATGGAAACGC TTTAGTGCCT GGAGATCGTG GCTTGTACAC CTGCTTGTAT 
CAGAACGAGG TCAACTCGGC CAACTCGTCC ATGCAACTGA GGATCGAACA TGAGCCCATT 
GTACTCCATC AGTATAACAA GGTGGCCTTT GATATCAGGG AGACAGCCGA GGTGGTTTGC 
AAGGTTCAGG CCTATCCCAA GCCCGAGTTC CAATGGCAGT TTGGCAATAA TCCATCGCCC 
CTGACCATGT CCTCCGATGG TCACTACGAG ATTAGTACCA CCACCGATAA CAACGACATC 
TATACGTCCG TGCTCAAGAT CAACTCGCTG ACTCATTCGG ATTACGGAGA ATACACCTGC 
CGGGTGGCCA ACACCCTGGA TACGATTCGA GCTCCCATTA GACTGCAGCA GAAAGGACCC 
CCAGAGAAAC CAACAAATCT GCGAGCCACC GAGGTGGGTC ACAATTACGT ATCACTGAGC 
TGGGATCCCG GATTCGATGG AGGTCTGAGC AAGACCAAGT TCTTTGTTAG CTATCGCCGT 
GTGGCCATGC CAAGGGAAGA ACAATTGATA CCGGATTGCG CCACTTTGGC CAACTCGAAT 
TCGGCTTGGG TGGAAGTCGA TTGCCAGCGG GATATTCCCT GCAAGGTGAC AGCCCTGGAG 
CAACACCAGA GTTATGCCTT TAAGGTCAAG GCTCTGAATC CCAAGAGCGA TTCACCGTAC 
TCGAGTGAAA TCATGGTGAC GACAAAGGTC AGCAGGATTC CGCCACCATT GCAGGTGACC 
TATGAGCCGA ATACTCGAAC TCTCGGCATC GATGTGGGGG CCACGTGCTT GAACCTGGTG 
GCCGTTGTGG AGTCCATGGT GAATGCAGAT TCTCCGATGG CCGCCTGGGA GGTGGTGACC 
ACCATGGATA ACCTGCAGCT GTCCGGAAAT GGACCCACCC ACAAGGAGAA GATCATCGAC 
AGAATAATTG GAGCGCGGCG AGTTGGCGGA GGACGTGCTC TGGGGCACAC CATCAGCGAG 
GACGAGGATG ACAATGGTTT GAACTCGCTG GCCCTCGAGG ACGAAAACAG TCCCACTGTG 
CGGGTCAAAT TGTGTCTGAG GACCAATCCA GAGCACTGCG GCGATTACAC GGACGCAGAA 
ATTGGCAGGC CGTATATAGC GGAGGCTAAT GCCCTGGCCA CGCCCACTCT CATTGCGATA 
ATCGTGTCCT GCGTGGTCTT TGCCCTCTTC GCTGGCCTAA TCCTGATGTT CTGCCGCTGC 
AAGCGCAATC AATCGAAGAA GAGCGCCGCC GCCAAAGATT ACGAGATGGA CTCGGTGCGT 
CCTTCGATTG TGGCCGCTCA GCAAAACCAG GCGCCACCAC CCTACTACCC CGCCTCTGGG 
CTGGACAACA AGGCATTGGA ACACTCAATG GATCTCGCCC TCAGCATGGA GGATCAGAAA 
ACGGCACTAT ACGCCACCCA GAATGGATAT AGCTATCATC CGGGAAGTGG GGTGGTGGGC 
GTTGGCATGG GCGGCGGCGT TGTGGGAGTG GGTGTTGGCG GCAGCGTGGT CAGCGGAATG 
GGCGGCGGAG TGGGCGGAAT CGGAGGCAGT GGCGTCGGCG TCAATGGAAT ACCTGGACTC 
AGCGCACACA CGATGCCCGG AAATGAATGG GTCAACATGG GCTACATGGA GAACAACTAT 
TCGAATTCGA ACAACGGCGG CAGTGTCAAC TCGCAGGACT CACTGTGGCA GGTGAAGATG 
TCAGCCGCGG CGGTGGGCAA CCAGCAGGGC ATGGTGCAGG CCCCAATGAA TCAGTATGTG 
GAACAGCAGC CCGCCTACGG ATACGATCCG TTGACCCATG GTGGCTATGG GGCGGTGGAC 
GACTACGCTC CCTATCCGCA CCTCACGGCC ACGCCCAGCC AGGTGGGCGA TGAGTACCAC 
AACTTGCGCA ACAGCCAGAA TCCGTCCCGG CAGGACTACT GCAGCGATCC CTATGCATCC 
GTGCAAAAGC CCAAAAAGCG AGTCGATCAG CATTTAGATT CACCATATCA CGACGTAAGC 
GGTCTGCCCA ATCCCTACAA CATGGAGCAT TTGGAACAGG ACGAGGTCCT TCCCCCGCAG 
CAGCACATGT CCTTGAGCTA CGACGACAGC TTCGAGGGCG AATATTCGAC AACGCCGAAT 
GCCAGAAACC GTCGCGTCAT ACGCGAGATT ATTGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260013.1
CDS	433..4431
Translation	MRRKTTTKGTAIVNSRSARRAATTICNFFLIALVALSSTTLADESIDTRENADLTLKCRFNDKYEANDFSFFWTRWTANPAQFDNVAIGEVQLSSGYRLDFQPERGIYDLQIKNVSYNRDNGRFECRIKAKGTGADVHQEFHNLTVLTPPHPPVISPGNIAVATEDKPMELTCSSIGGSPDPTITWYREGSNTPLPATVLKGGTKDQPTNATLSIIPRREDDGAKYKCVVRNRAMNEGKRLEATATLNVNYYPRVEVGPENPLRVERDRTAKLECNVDAKPKVPNVRWNRNGRFISSSLVHTIHRVSVQDAGKYTCIADNGLGKTGEQELILDILYPPMVVIESKTREAEEGDTVTIRCNVTANPAPVTIEWLKENSPDFRYNGDVLTLTSVRADHAGNYICRAVNIMQSQGMERSERVGNSTVALLVRHRPGQAYITPNKPVVHVGNGVTLTCSANPPGWPVPQYRWFRDMDGEFSSTQKILAQGPQYSIPKAHLGNEGKYHCHAVNELGIGMMATIVLEIHQPPQFLAKLQQHMTRRVADTDYTVTCSAKGKPAPSVKWLKDAVEILPEENLYEVQTNPDQGLNGMVTVQSQLKFRGKARPNGNALVPGDRGLYTCLYQNEVNSANSSMQLRIEHEPIVLHQYNKVAFDIRETAEVVCKVQAYPKPEFQWQFGNNPSPLTMSSDGHYEISTTTDNNDIYTSVLKINSLTHSDYGEYTCRVANTLDTIRAPIRLQQKGPPEKPTNLRATEVGHNYVSLSWDPGFDGGLSKTKFFVSYRRVAMPREEQLIPDCATLANSNSAWVEVDCQRDIPCKVTALEQHQSYAFKVKALNPKSDSPYSSEIMVTTKVSRIPPPLQVTYEPNTRTLGIDVGATCLNLVAVVESMVNADSPMAAWEVVTTMDNLQLSGNGPTHKEKIIDRIIGARRVGGGRALGHTISEDEDDNGLNSLALEDENSPTVRVKLCLRTNPEHCGDYTDAEIGRPYIAEANALATPTLIAIIVSCVVFALFAGLILMFCRCKRNQSKKSAAAKDYEMDSVRPSIVAAQQNQAPPPYYPASGLDNKALEHSMDLALSMEDQKTALYATQNGYSYHPGSGVVGVGMGGGVVGVGVGGSVVSGMGGGVGGIGGSGVGVNGIPGLSAHTMPGNEWVNMGYMENNYSNSNNGGSVNSQDSLWQVKMSAAAVGNQQGMVQAPMNQYVEQQPAYGYDPLTHGGYGAVDDYAPYPHLTATPSQVGDEYHNLRNSQNPSRQDYCSDPYASVQKPKKRVDQHLDSPYHDVSGLPNPYNMEHLEQDEVLPPQQHMSLSYDDSFEGEYSTTPNARNRRVIREIIV

Target ORF information:

RefSeq Version	NM_001273084.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster echinoid (ed), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273084.1

ORF Insert Sequence:

ATGAGGCGAA AAACTACCAC GAAAGGGACG GCAATAGTCA ATAGCAGGAG TGCGAGGAGA 
GCAGCAACGA CTATATGCAA CTTTTTTCTC ATCGCACTCG TAGCTCTGAG TTCCACGACT 
CTGGCCGATG AATCCATTGA CACACGTGAG AATGCTGACT TAACCCTCAA GTGCCGGTTT 
AACGACAAGT ATGAGGCGAA CGATTTCTCT TTCTTCTGGA CCCGCTGGAC AGCGAATCCC 
GCCCAATTCG ATAACGTAGC CATCGGTGAA GTGCAGTTGA GTTCCGGCTA TAGACTCGAC 
TTTCAACCGG AGCGTGGCAT TTACGATCTG CAGATCAAGA ATGTGTCCTA TAACCGTGAC 
AATGGACGCT TCGAGTGCCG CATCAAGGCC AAGGGAACGG GCGCAGACGT CCATCAGGAG 
TTCCACAATC TCACGGTGCT CACGCCACCT CATCCACCCG TGATCTCACC CGGAAACATA 
GCCGTGGCCA CCGAGGACAA GCCCATGGAG CTGACATGCA GCAGCATCGG TGGTTCCCCC 
GATCCCACCA TAACTTGGTA TCGCGAGGGT TCGAATACGC CCCTGCCAGC GACGGTGTTA 
AAGGGCGGTA CCAAGGATCA GCCCACCAAT GCCACGCTAT CCATAATTCC TCGCAGAGAA 
GATGACGGTG CCAAGTACAA GTGTGTAGTC AGAAATCGTG CCATGAACGA GGGAAAGCGA 
TTGGAGGCCA CTGCCACATT GAATGTTAAC TATTACCCCC GAGTCGAGGT TGGACCCGAG 
AATCCTTTGC GGGTGGAACG TGACCGGACT GCCAAACTGG AGTGTAATGT GGATGCCAAG 
CCGAAGGTTC CCAATGTCCG TTGGAATCGC AATGGTCGGT TCATCAGCTC CTCATTAGTT 
CACACGATCC ATCGGGTGAG TGTTCAAGAT GCCGGCAAGT ATACCTGCAT TGCGGACAAC 
GGTCTTGGAA AGACCGGAGA ACAGGAGCTC ATCCTGGATA TCCTGTATCC ACCCATGGTC 
GTGATAGAGT CCAAGACGCG AGAGGCCGAA GAGGGCGATA CAGTGACCAT TCGCTGCAAT 
GTCACAGCCA ATCCTGCACC CGTGACCATT GAGTGGCTCA AGGAGAACAG TCCCGACTTC 
CGCTACAATG GCGACGTACT GACCCTAACT TCCGTGCGAG CCGATCATGC CGGAAACTAC 
ATCTGCCGTG CCGTGAACAT AATGCAGAGC CAGGGAATGG AGCGCAGTGA ACGAGTGGGT 
AACTCCACGG TGGCCCTGCT AGTTCGCCAT CGACCAGGAC AGGCTTACAT TACACCCAAT 
AAGCCCGTGG TTCATGTGGG TAATGGTGTC ACCTTGACCT GCTCCGCTAA TCCGCCAGGC 
TGGCCAGTGC CCCAGTACAG ATGGTTCAGG GATATGGATG GAGAGTTCTC CAGCACGCAG 
AAAATCCTTG CACAAGGCCC TCAGTACTCG ATACCCAAAG CTCACTTGGG TAACGAGGGT 
AAATACCATT GTCATGCTGT AAATGAACTG GGCATCGGAA TGATGGCCAC CATTGTCCTG 
GAGATCCACC AGCCTCCGCA GTTCTTGGCC AAGCTACAGC AGCATATGAC TCGCAGAGTG 
GCCGATACGG ATTATACGGT CACGTGCAGT GCCAAGGGAA AGCCAGCACC CAGTGTTAAA 
TGGCTGAAGG ACGCAGTGGA AATCCTGCCC GAGGAAAATC TCTACGAAGT GCAGACCAAT 
CCCGATCAGG GCCTCAACGG CATGGTCACG GTTCAGAGTC AACTTAAGTT CCGAGGAAAG 
GCCAGACCGA ATGGAAACGC TTTAGTGCCT GGAGATCGTG GCTTGTACAC CTGCTTGTAT 
CAGAACGAGG TCAACTCGGC CAACTCGTCC ATGCAACTGA GGATCGAACA TGAGCCCATT 
GTACTCCATC AGTATAACAA GGTGGCCTTT GATATCAGGG AGACAGCCGA GGTGGTTTGC 
AAGGTTCAGG CCTATCCCAA GCCCGAGTTC CAATGGCAGT TTGGCAATAA TCCATCGCCC 
CTGACCATGT CCTCCGATGG TCACTACGAG ATTAGTACCA CCACCGATAA CAACGACATC 
TATACGTCCG TGCTCAAGAT CAACTCGCTG ACTCATTCGG ATTACGGAGA ATACACCTGC 
CGGGTGGCCA ACACCCTGGA TACGATTCGA GCTCCCATTA GACTGCAGCA GAAAGGACCC 
CCAGAGAAAC CAACAAATCT GCGAGCCACC GAGGTGGGTC ACAATTACGT ATCACTGAGC 
TGGGATCCCG GATTCGATGG AGGTCTGAGC AAGACCAAGT TCTTTGTTAG CTATCGCCGT 
GTGGCCATGC CAAGGGAAGA ACAATTGATA CCGGATTGCG CCACTTTGGC CAACTCGAAT 
TCGGCTTGGG TGGAAGTCGA TTGCCAGCGG GATATTCCCT GCAAGGTGAC AGCCCTGGAG 
CAACACCAGA GTTATGCCTT TAAGGTCAAG GCTCTGAATC CCAAGAGCGA TTCACCGTAC 
TCGAGTGAAA TCATGGTGAC GACAAAGGTC AGCAGGATTC CGCCACCATT GCAGGTGACC 
TATGAGCCGA ATACTCGAAC TCTCGGCATC GATGTGGGGG CCACGTGCTT GAACCTGGTG 
GCCGTTGTGG AGTCCATGGT GAATGCAGAT TCTCCGATGG CCGCCTGGGA GGTGGTGACC 
ACCATGGATA ACCTGCAGCT GTCCGGAAAT GGACCCACCC ACAAGGAGAA GATCATCGAC 
AGAATAATTG GAGCGCGGCG AGTTGGCGGA GGACGTGCTC TGGGGCACAC CATCAGCGAG 
GACGAGGATG ACAATGGTTT GAACTCGCTG GCCCTCGAGG ACGAAAACAG TCCCACTGTG 
CGGGTCAAAT TGTGTCTGAG GACCAATCCA GAGCACTGCG GCGATTACAC GGACGCAGAA 
ATTGGCAGGC CGTATATAGC GGAGGCTAAT GCCCTGGCCA CGCCCACTCT CATTGCGATA 
ATCGTGTCCT GCGTGGTCTT TGCCCTCTTC GCTGGCCTAA TCCTGATGTT CTGCCGCTGC 
AAGCGCAATC AATCGAAGAA GAGCGCCGCC GCCAAAGATT ACGAGATGGA CTCGGTGCGT 
CCTTCGATTG TGGCCGCTCA GCAAAACCAG GCGCCACCAC CCTACTACCC CGCCTCTGGG 
CTGGACAACA AGGCATTGGA ACACTCAATG GATCTCGCCC TCAGCATGGA GGATCAGAAA 
ACGGCACTAT ACGCCACCCA GAATGGATAT AGCTATCATC CGGGAAGTGG GGTGGTGGGC 
GTTGGCATGG GCGGCGGCGT TGTGGGAGTG GGTGTTGGCG GCAGCGTGGT CAGCGGAATG 
GGCGGCGGAG TGGGCGGAAT CGGAGGCAGT GGCGTCGGCG TCAATGGAAT ACCTGGACTC 
AGCGCACACA CGATGCCCGG AAATGAATGG GTCAACATGG GCTACATGGA GAACAACTAT 
TCGAATTCGA ACAACGGCGG CAGTGTCAAC TCGCAGGACT CACTGTGGCA GGTGAAGATG 
TCAGCCGCGG CGGTGGGCAA CCAGCAGGGC ATGGTGCAGG CCCCAATGAA TCAGTATGTG 
GAACAGCAGC CCGCCTACGG ATACGATCCG TTGACCCATG GTGGCTATGG GGCGGTGGAC 
GACTACGCTC CCTATCCGCA CCTCACGGCC ACGCCCAGCC AGGTGGGCGA TGAGTACCAC 
AACTTGCGCA ACAGCCAGAA TCCGTCCCGG CAGGACTACT GCAGCGATCC CTATGCATCC 
GTGCAAAAGC CCAAAAAGCG AGTCGATCAG CATTTAGATT CACCATATCA CGACGTAAGC 
GGTCTGCCCA ATCCCTACAA CATGGAGCAT TTGGAACAGG ACGAGGTCCT TCCCCCGCAG 
CAGCACATGT CCTTGAGCTA CGACGACAGC TTCGAGGGCG AATATTCGAC AACGCCGAAT 
GCCAGAAACC GTCGCGTCAT ACGCGAGATT ATTGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ed ( NM_001273085.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260014.1 Drosophila melanogaster echinoid (ed), transcript variant C, mRNA.

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CloneID	OFa16200
Clone ID Related Accession (Same CDS sequence)	NM_001273085.1
Accession Version	NM_001273085.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	echinoid, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGGCGAA AAACTACCAC GAAAGGGACG GCAATAGTCA ATAGCAGGAG TGCGAGGAGA 
GCAGCAACGA CTATATGCAA CTTTTTTCTC ATCGCACTCG TAGCTCTGAG TTCCACGACT 
CTGGCCGATG AATCCATTGA CACACGTGAG AATGCTGACT TAACCCTCAA GTGCCGGTTT 
AACGACAAGT ATGAGGCGAA CGATTTCTCT TTCTTCTGGA CCCGCTGGAC AGCGAATCCC 
GCCCAATTCG ATAACGTAGC CATCGGTGAA GTGCAGTTGA GTTCCGGCTA TAGACTCGAC 
TTTCAACCGG AGCGTGGCAT TTACGATCTG CAGATCAAGA ATGTGTCCTA TAACCGTGAC 
AATGGACGCT TCGAGTGCCG CATCAAGGCC AAGGGAACGG GCGCAGACGT CCATCAGGAG 
TTCCACAATC TCACGGTGCT CACGCCACCT CATCCACCCG TGATCTCACC CGGAAACATA 
GCCGTGGCCA CCGAGGACAA GCCCATGGAG CTGACATGCA GCAGCATCGG TGGTTCCCCC 
GATCCCACCA TAACTTGGTA TCGCGAGGGT TCGAATACGC CCCTGCCAGC GACGGTGTTA 
AAGGGCGGTA CCAAGGATCA GCCCACCAAT GCCACGCTAT CCATAATTCC TCGCAGAGAA 
GATGACGGTG CCAAGTACAA GTGTGTAGTC AGAAATCGTG CCATGAACGA GGGAAAGCGA 
TTGGAGGCCA CTGCCACATT GAATGTTAAC TATTACCCCC GAGTCGAGGT TGGACCCGAG 
AATCCTTTGC GGGTGGAACG TGACCGGACT GCCAAACTGG AGTGTAATGT GGATGCCAAG 
CCGAAGGTTC CCAATGTCCG TTGGAATCGC AATGGTCGGT TCATCAGCTC CTCATTAGTT 
CACACGATCC ATCGGGTGAG TGTTCAAGAT GCCGGCAAGT ATACCTGCAT TGCGGACAAC 
GGTCTTGGAA AGACCGGAGA ACAGGAGCTC ATCCTGGATA TCCTGTATCC ACCCATGGTC 
GTGATAGAGT CCAAGACGCG AGAGGCCGAA GAGGGCGATA CAGTGACCAT TCGCTGCAAT 
GTCACAGCCA ATCCTGCACC CGTGACCATT GAGTGGCTCA AGGAGAACAG TCCCGACTTC 
CGCTACAATG GCGACGTACT GACCCTAACT TCCGTGCGAG CCGATCATGC CGGAAACTAC 
ATCTGCCGTG CCGTGAACAT AATGCAGAGC CAGGGAATGG AGCGCAGTGA ACGAGTGGGT 
AACTCCACGG TGGCCCTGCT AGTTCGCCAT CGACCAGGAC AGGCTTACAT TACACCCAAT 
AAGCCCGTGG TTCATGTGGG TAATGGTGTC ACCTTGACCT GCTCCGCTAA TCCGCCAGGC 
TGGCCAGTGC CCCAGTACAG ATGGTTCAGG GATATGGATG GAGAGTTCTC CAGCACGCAG 
AAAATCCTTG CACAAGGCCC TCAGTACTCG ATACCCAAAG CTCACTTGGG TAACGAGGGT 
AAATACCATT GTCATGCTGT AAATGAACTG GGCATCGGAA TGATGGCCAC CATTGTCCTG 
GAGATCCACC AGCCTCCGCA GTTCTTGGCC AAGCTACAGC AGCATATGAC TCGCAGAGTG 
GCCGATACGG ATTATACGGT CACGTGCAGT GCCAAGGGAA AGCCAGCACC CAGTGTTAAA 
TGGCTGAAGG ACGCAGTGGA AATCCTGCCC GAGGAAAATC TCTACGAAGT GCAGACCAAT 
CCCGATCAGG GCCTCAACGG CATGGTCACG GTTCAGAGTC AACTTAAGTT CCGAGGAAAG 
GCCAGACCGA ATGGAAACGC TTTAGTGCCT GGAGATCGTG GCTTGTACAC CTGCTTGTAT 
CAGAACGAGG TCAACTCGGC CAACTCGTCC ATGCAACTGA GGATCGAACA TGAGCCCATT 
GTACTCCATC AGTATAACAA GGTGGCCTTT GATATCAGGG AGACAGCCGA GGTGGTTTGC 
AAGGTTCAGG CCTATCCCAA GCCCGAGTTC CAATGGCAGT TTGGCAATAA TCCATCGCCC 
CTGACCATGT CCTCCGATGG TCACTACGAG ATTAGTACCA CCACCGATAA CAACGACATC 
TATACGTCCG TGCTCAAGAT CAACTCGCTG ACTCATTCGG ATTACGGAGA ATACACCTGC 
CGGGTGGCCA ACACCCTGGA TACGATTCGA GCTCCCATTA GACTGCAGCA GAAAGGACCC 
CCAGAGAAAC CAACAAATCT GCGAGCCACC GAGGTGGGTC ACAATTACGT ATCACTGAGC 
TGGGATCCCG GATTCGATGG AGGTCTGAGC AAGACCAAGT TCTTTGTTAG CTATCGCCGT 
GTGGCCATGC CAAGGGAAGA ACAATTGATA CCGGATTGCG CCACTTTGGC CAACTCGAAT 
TCGGCTTGGG TGGAAGTCGA TTGCCAGCGG GATATTCCCT GCAAGGTGAC AGCCCTGGAG 
CAACACCAGA GTTATGCCTT TAAGGTCAAG GCTCTGAATC CCAAGAGCGA TTCACCGTAC 
TCGAGTGAAA TCATGGTGAC GACAAAGGTC AGCAGGATTC CGCCACCATT GCAGGTGACC 
TATGAGCCGA ATACTCGAAC TCTCGGCATC GATGTGGGGG CCACGTGCTT GAACCTGGTG 
GCCGTTGTGG AGTCCATGGT GAATGCAGAT TCTCCGATGG CCGCCTGGGA GGTGGTGACC 
ACCATGGATA ACCTGCAGCT GTCCGGAAAT GGACCCACCC ACAAGGAGAA GATCATCGAC 
AGAATAATTG GAGCGCGGCG AGTTGGCGGA GGACGTGCTC TGGGGCACAC CATCAGCGAG 
GACGAGGATG ACAATGGTTT GAACTCGCTG GCCCTCGAGG ACGAAAACAG TCCCACTGTG 
CGGGTCAAAT TGTGTCTGAG GACCAATCCA GAGCACTGCG GCGATTACAC GGACGCAGAA 
ATTGGCAGGC CGTATATAGC GGAGGCTAAT GCCCTGGCCA CGCCCACTCT CATTGCGATA 
ATCGTGTCCT GCGTGGTCTT TGCCCTCTTC GCTGGCCTAA TCCTGATGTT CTGCCGCTGC 
AAGCGCAATC AATCGAAGAA GAGCGCCGCC GCCAAAGATT ACGAGATGGA CTCGGTGCGT 
CCTTCGATTG TGGCCGCTCA GCAAAACCAG GCGCCACCAC CCTACTACCC CGCCTCTGGG 
CTGGACAACA AGGCATTGGA ACACTCAATG GATCTCGCCC TCAGCATGGA GGATCAGAAA 
ACGGCACTAT ACGCCACCCA GAATGGATAT AGCTATCATC CGGGAAGTGG GGTGGTGGGC 
GTTGGCATGG GCGGCGGCGT TGTGGGAGTG GGTGTTGGCG GCAGCGTGGT CAGCGGAATG 
GGCGGCGGAG TGGGCGGAAT CGGAGGCAGT GGCGTCGGCG TCAATGGAAT ACCTGGACTC 
AGCGCACACA CGATGCCCGG AAATGAATGG GTCAACATGG GCTACATGGA GAACAACTAT 
TCGAATTCGA ACAACGGCGG CAGTGTCAAC TCGCAGGACT CACTGTGGCA GGTGAAGATG 
TCAGCCGCGG CGGTGGGCAA CCAGCAGGGC ATGGTGCAGG CCCCAATGAA TCAGTATGTG 
GAACAGCAGC CCGCCTACGG ATACGATCCG TTGACCCATG GTGGCTATGG GGCGGTGGAC 
GACTACGCTC CCTATCCGCA CCTCACGGCC ACGCCCAGCC AGGTGGGCGA TGAGTACCAC 
AACTTGCGCA ACAGCCAGAA TCCGTCCCGG CAGGACTACT GCAGCGATCC CTATGCATCC 
GTGCAAAAGC CCAAAAAGCG AGTCGATCAG CATTTAGCGG ACATTATACA GGATGTTACG 
CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260014.1
CDS	433..4278
Translation	MRRKTTTKGTAIVNSRSARRAATTICNFFLIALVALSSTTLADESIDTRENADLTLKCRFNDKYEANDFSFFWTRWTANPAQFDNVAIGEVQLSSGYRLDFQPERGIYDLQIKNVSYNRDNGRFECRIKAKGTGADVHQEFHNLTVLTPPHPPVISPGNIAVATEDKPMELTCSSIGGSPDPTITWYREGSNTPLPATVLKGGTKDQPTNATLSIIPRREDDGAKYKCVVRNRAMNEGKRLEATATLNVNYYPRVEVGPENPLRVERDRTAKLECNVDAKPKVPNVRWNRNGRFISSSLVHTIHRVSVQDAGKYTCIADNGLGKTGEQELILDILYPPMVVIESKTREAEEGDTVTIRCNVTANPAPVTIEWLKENSPDFRYNGDVLTLTSVRADHAGNYICRAVNIMQSQGMERSERVGNSTVALLVRHRPGQAYITPNKPVVHVGNGVTLTCSANPPGWPVPQYRWFRDMDGEFSSTQKILAQGPQYSIPKAHLGNEGKYHCHAVNELGIGMMATIVLEIHQPPQFLAKLQQHMTRRVADTDYTVTCSAKGKPAPSVKWLKDAVEILPEENLYEVQTNPDQGLNGMVTVQSQLKFRGKARPNGNALVPGDRGLYTCLYQNEVNSANSSMQLRIEHEPIVLHQYNKVAFDIRETAEVVCKVQAYPKPEFQWQFGNNPSPLTMSSDGHYEISTTTDNNDIYTSVLKINSLTHSDYGEYTCRVANTLDTIRAPIRLQQKGPPEKPTNLRATEVGHNYVSLSWDPGFDGGLSKTKFFVSYRRVAMPREEQLIPDCATLANSNSAWVEVDCQRDIPCKVTALEQHQSYAFKVKALNPKSDSPYSSEIMVTTKVSRIPPPLQVTYEPNTRTLGIDVGATCLNLVAVVESMVNADSPMAAWEVVTTMDNLQLSGNGPTHKEKIIDRIIGARRVGGGRALGHTISEDEDDNGLNSLALEDENSPTVRVKLCLRTNPEHCGDYTDAEIGRPYIAEANALATPTLIAIIVSCVVFALFAGLILMFCRCKRNQSKKSAAAKDYEMDSVRPSIVAAQQNQAPPPYYPASGLDNKALEHSMDLALSMEDQKTALYATQNGYSYHPGSGVVGVGMGGGVVGVGVGGSVVSGMGGGVGGIGGSGVGVNGIPGLSAHTMPGNEWVNMGYMENNYSNSNNGGSVNSQDSLWQVKMSAAAVGNQQGMVQAPMNQYVEQQPAYGYDPLTHGGYGAVDDYAPYPHLTATPSQVGDEYHNLRNSQNPSRQDYCSDPYASVQKPKKRVDQHLADIIQDVTL

Target ORF information:

RefSeq Version	NM_001273085.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster echinoid (ed), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273085.1

ORF Insert Sequence:

ATGAGGCGAA AAACTACCAC GAAAGGGACG GCAATAGTCA ATAGCAGGAG TGCGAGGAGA 
GCAGCAACGA CTATATGCAA CTTTTTTCTC ATCGCACTCG TAGCTCTGAG TTCCACGACT 
CTGGCCGATG AATCCATTGA CACACGTGAG AATGCTGACT TAACCCTCAA GTGCCGGTTT 
AACGACAAGT ATGAGGCGAA CGATTTCTCT TTCTTCTGGA CCCGCTGGAC AGCGAATCCC 
GCCCAATTCG ATAACGTAGC CATCGGTGAA GTGCAGTTGA GTTCCGGCTA TAGACTCGAC 
TTTCAACCGG AGCGTGGCAT TTACGATCTG CAGATCAAGA ATGTGTCCTA TAACCGTGAC 
AATGGACGCT TCGAGTGCCG CATCAAGGCC AAGGGAACGG GCGCAGACGT CCATCAGGAG 
TTCCACAATC TCACGGTGCT CACGCCACCT CATCCACCCG TGATCTCACC CGGAAACATA 
GCCGTGGCCA CCGAGGACAA GCCCATGGAG CTGACATGCA GCAGCATCGG TGGTTCCCCC 
GATCCCACCA TAACTTGGTA TCGCGAGGGT TCGAATACGC CCCTGCCAGC GACGGTGTTA 
AAGGGCGGTA CCAAGGATCA GCCCACCAAT GCCACGCTAT CCATAATTCC TCGCAGAGAA 
GATGACGGTG CCAAGTACAA GTGTGTAGTC AGAAATCGTG CCATGAACGA GGGAAAGCGA 
TTGGAGGCCA CTGCCACATT GAATGTTAAC TATTACCCCC GAGTCGAGGT TGGACCCGAG 
AATCCTTTGC GGGTGGAACG TGACCGGACT GCCAAACTGG AGTGTAATGT GGATGCCAAG 
CCGAAGGTTC CCAATGTCCG TTGGAATCGC AATGGTCGGT TCATCAGCTC CTCATTAGTT 
CACACGATCC ATCGGGTGAG TGTTCAAGAT GCCGGCAAGT ATACCTGCAT TGCGGACAAC 
GGTCTTGGAA AGACCGGAGA ACAGGAGCTC ATCCTGGATA TCCTGTATCC ACCCATGGTC 
GTGATAGAGT CCAAGACGCG AGAGGCCGAA GAGGGCGATA CAGTGACCAT TCGCTGCAAT 
GTCACAGCCA ATCCTGCACC CGTGACCATT GAGTGGCTCA AGGAGAACAG TCCCGACTTC 
CGCTACAATG GCGACGTACT GACCCTAACT TCCGTGCGAG CCGATCATGC CGGAAACTAC 
ATCTGCCGTG CCGTGAACAT AATGCAGAGC CAGGGAATGG AGCGCAGTGA ACGAGTGGGT 
AACTCCACGG TGGCCCTGCT AGTTCGCCAT CGACCAGGAC AGGCTTACAT TACACCCAAT 
AAGCCCGTGG TTCATGTGGG TAATGGTGTC ACCTTGACCT GCTCCGCTAA TCCGCCAGGC 
TGGCCAGTGC CCCAGTACAG ATGGTTCAGG GATATGGATG GAGAGTTCTC CAGCACGCAG 
AAAATCCTTG CACAAGGCCC TCAGTACTCG ATACCCAAAG CTCACTTGGG TAACGAGGGT 
AAATACCATT GTCATGCTGT AAATGAACTG GGCATCGGAA TGATGGCCAC CATTGTCCTG 
GAGATCCACC AGCCTCCGCA GTTCTTGGCC AAGCTACAGC AGCATATGAC TCGCAGAGTG 
GCCGATACGG ATTATACGGT CACGTGCAGT GCCAAGGGAA AGCCAGCACC CAGTGTTAAA 
TGGCTGAAGG ACGCAGTGGA AATCCTGCCC GAGGAAAATC TCTACGAAGT GCAGACCAAT 
CCCGATCAGG GCCTCAACGG CATGGTCACG GTTCAGAGTC AACTTAAGTT CCGAGGAAAG 
GCCAGACCGA ATGGAAACGC TTTAGTGCCT GGAGATCGTG GCTTGTACAC CTGCTTGTAT 
CAGAACGAGG TCAACTCGGC CAACTCGTCC ATGCAACTGA GGATCGAACA TGAGCCCATT 
GTACTCCATC AGTATAACAA GGTGGCCTTT GATATCAGGG AGACAGCCGA GGTGGTTTGC 
AAGGTTCAGG CCTATCCCAA GCCCGAGTTC CAATGGCAGT TTGGCAATAA TCCATCGCCC 
CTGACCATGT CCTCCGATGG TCACTACGAG ATTAGTACCA CCACCGATAA CAACGACATC 
TATACGTCCG TGCTCAAGAT CAACTCGCTG ACTCATTCGG ATTACGGAGA ATACACCTGC 
CGGGTGGCCA ACACCCTGGA TACGATTCGA GCTCCCATTA GACTGCAGCA GAAAGGACCC 
CCAGAGAAAC CAACAAATCT GCGAGCCACC GAGGTGGGTC ACAATTACGT ATCACTGAGC 
TGGGATCCCG GATTCGATGG AGGTCTGAGC AAGACCAAGT TCTTTGTTAG CTATCGCCGT 
GTGGCCATGC CAAGGGAAGA ACAATTGATA CCGGATTGCG CCACTTTGGC CAACTCGAAT 
TCGGCTTGGG TGGAAGTCGA TTGCCAGCGG GATATTCCCT GCAAGGTGAC AGCCCTGGAG 
CAACACCAGA GTTATGCCTT TAAGGTCAAG GCTCTGAATC CCAAGAGCGA TTCACCGTAC 
TCGAGTGAAA TCATGGTGAC GACAAAGGTC AGCAGGATTC CGCCACCATT GCAGGTGACC 
TATGAGCCGA ATACTCGAAC TCTCGGCATC GATGTGGGGG CCACGTGCTT GAACCTGGTG 
GCCGTTGTGG AGTCCATGGT GAATGCAGAT TCTCCGATGG CCGCCTGGGA GGTGGTGACC 
ACCATGGATA ACCTGCAGCT GTCCGGAAAT GGACCCACCC ACAAGGAGAA GATCATCGAC 
AGAATAATTG GAGCGCGGCG AGTTGGCGGA GGACGTGCTC TGGGGCACAC CATCAGCGAG 
GACGAGGATG ACAATGGTTT GAACTCGCTG GCCCTCGAGG ACGAAAACAG TCCCACTGTG 
CGGGTCAAAT TGTGTCTGAG GACCAATCCA GAGCACTGCG GCGATTACAC GGACGCAGAA 
ATTGGCAGGC CGTATATAGC GGAGGCTAAT GCCCTGGCCA CGCCCACTCT CATTGCGATA 
ATCGTGTCCT GCGTGGTCTT TGCCCTCTTC GCTGGCCTAA TCCTGATGTT CTGCCGCTGC 
AAGCGCAATC AATCGAAGAA GAGCGCCGCC GCCAAAGATT ACGAGATGGA CTCGGTGCGT 
CCTTCGATTG TGGCCGCTCA GCAAAACCAG GCGCCACCAC CCTACTACCC CGCCTCTGGG 
CTGGACAACA AGGCATTGGA ACACTCAATG GATCTCGCCC TCAGCATGGA GGATCAGAAA 
ACGGCACTAT ACGCCACCCA GAATGGATAT AGCTATCATC CGGGAAGTGG GGTGGTGGGC 
GTTGGCATGG GCGGCGGCGT TGTGGGAGTG GGTGTTGGCG GCAGCGTGGT CAGCGGAATG 
GGCGGCGGAG TGGGCGGAAT CGGAGGCAGT GGCGTCGGCG TCAATGGAAT ACCTGGACTC 
AGCGCACACA CGATGCCCGG AAATGAATGG GTCAACATGG GCTACATGGA GAACAACTAT 
TCGAATTCGA ACAACGGCGG CAGTGTCAAC TCGCAGGACT CACTGTGGCA GGTGAAGATG 
TCAGCCGCGG CGGTGGGCAA CCAGCAGGGC ATGGTGCAGG CCCCAATGAA TCAGTATGTG 
GAACAGCAGC CCGCCTACGG ATACGATCCG TTGACCCATG GTGGCTATGG GGCGGTGGAC 
GACTACGCTC CCTATCCGCA CCTCACGGCC ACGCCCAGCC AGGTGGGCGA TGAGTACCAC 
AACTTGCGCA ACAGCCAGAA TCCGTCCCGG CAGGACTACT GCAGCGATCC CTATGCATCC 
GTGCAAAAGC CCAAAAAGCG AGTCGATCAG CATTTAGCGG ACATTATACA GGATGTTACG 
CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ed cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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