fred cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	fred
Entrez Gene ID	33613
Full Name	friend of echinoid
Synonyms	CG12675,CG31774,CG3390,CG3393,CT11413,Dmel\CG31774,Fred
General protein information	Preferred Names friend of echinoid Names CG31774 gene product from transcript CG31774-RF CG31774-PF CG31774-PG CG31774-PH fred-PF fred-PG fred-PH
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 24C9-24D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_134968.4	NP_608812.3	friend of echinoid, isoform H
NM_164569.4	NP_722954.3	friend of echinoid, isoform G
NM_001258937.2	NP_001245866.1	friend of echinoid, isoform F

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

differential levels of Ed and Fred between stationary and rotating cells at the initiation of rotation create a permissive environment for cell movement, and uniform levels in these two populations later contribute to stopping the movement
Title: The cell adhesion molecules Echinoid and Friend of Echinoid coordinate cell adhesion and cell signaling to regulate the fidelity of ommatidial rotation in the Drosophila eye.

acts in close concert with the Notch signaling pathway
Title: The Drosophila IgC2 domain protein Friend-of-Echinoid, a paralogue of Echinoid, limits the number of sensory organ precursors in the wing disc and interacts with the Notch signaling pathway.

The following fred gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fred cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13700	NM_164569.4 Latest version!	Drosophila melanogaster friend of echinoid (fred), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa16205	NM_001258937.2 Latest version!	Drosophila melanogaster friend of echinoid (fred), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa21586	NM_134968.4 Latest version!	Drosophila melanogaster friend of echinoid (fred), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fred ( NM_164569.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_722954.3 Drosophila melanogaster friend of echinoid (fred), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa13700
Clone ID Related Accession (Same CDS sequence)	NM_164569.4
Accession Version	NM_164569.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	friend of echinoid, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On May 8, 2012 this sequence version replaced NM_164569.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGACCA TAAAGCTGCT CATTATCGGT CAACTTTGGC TCTCGATTGG CCTGATCTCC 
GGGGACGATT CGCTGGACAC GCGTGAGGGC GTCGATCTGG TGCTCAAGTG CCGCTTCACC 
GAGCATTACG ACTCGACCGA CTTCACCTTC TACTGGGCGC GATGGACCTG CTGCCCCACA 
TTGTTCGAGA ACGTGGCCAT TGGCGATGTG CAGCTCAACT CGAATTATCG CTTGGACTTC 
CGACCCAGTC GCGGAATTTA CGATTTGCAA ATCAAAAACA CATCTTATAA TCGAGATAAT 
GGACGCTTTG AGTGCCGCAT CAAGGCGAAG GGAACGGGCG CGGATGTCCA TCAGGAGTTC 
TACAATCTGA CCGTGCTGAC GGCTCCACAT CCGCCGATGG TCACTCCCGG CAATTTAGCA 
GTGGCCACCG AAGAGAAGCC ATTGGAGTTG ACCTGCAGCA GTATTGGTGG CTCACCAGAT 
CCAATGATAA CCTGGTACCG AGAGGGCAGC ACAGTGCCAC TGCAGTCGTA TGCTCTGAAG 
GGCGGATCGA AGAACCACTA TACAAACGCC ACACTCCAAA TTGTTCCCCG ACGGGCCGAC 
GATGGCGCCA AGTACAAATG CGTCGTCTGG AATCGTGCCA TGCCCGAGGG TCATATGCTG 
GAGACCAGCG TCACGCTGAA TGTCAACTAC TATCCCCGAG TGGAGGTCGG ACCCCAGAAT 
CCTTTAAAAG TAGAACGGGA TCATGTGGCG AAACTGGACT GCAGAGTGGA TGCCAAACCG 
ATGGTTAGCA ATGTGCGATG GTCCCGAAAT GGTCAATATG TGAGTGCCAC GCCCACCCAC 
ACCATATATA GAGTAAATCG CCATCATGCC GGGAAGTACA CCTGCAGTGC GGACAACGGC 
TTGGGCAAAA CTGGCGAAAA AGATATAGTC TTGGATGTTC TATATCCGCC GATTGTGTTT 
ATCGAATCGA AAACGCACGA AGCGGAGGAA GGTGAAACGG TTTTGATTCG TTGCAATGTC 
ACCGCAAATC CATCGCCCAT CAATGTCGAA TGGCTAAAAG AAGGAGCTCC GGACTTCCGA 
TACACTGGCG AGCTCTTAAC CCTCGGTTCA GTGAGGGCTG AGCACGCCGG AAACTATATC 
TGCAGATCGG TGAACATTAT GCAACCGTTC AGTTCGAAAC GTGTAGAGGG TGTGGGCAAT 
TCCACGGTGG CTCTATTGGT TCGACATCGG CCGGGCCAGG CTTATATAAC TCCGAATAAG 
CCAGTCGTTC ATGTGGGCAA TGGTGTGACT CTAACCTGTT CAGCGAATCC GCCCGGATGG 
CCTGTACCCC AATACCGATG GTTCCGAGAT ATGGATGGTG ACATTGGAAA TACACAGAAA 
ATTTTAGCGC AAGGTCCACA GTACTCTATA CCGAAAGCTC ACTTGGGAAG CGAGGGCAAG 
TACCACTGTC ATGCGGTCAA CGAGTTGGGA ATTGGTAAAA TAGCCACCAT AATCTTAGAG 
GTACATCAGC CACCCCAGTT TCTGGCCAAA CTACAGCAAC ATATGACACG TCGGGTGGGC 
GATGTAGACT ATGCGGTCAC CTGCAGTGCC AAGGGAAAGC CTACACCGCA AATTCGATGG 
ATCAAAGATG GTACCGAGAT TCTGCCCACT CGCAAGATGT TTGATATAAG AACTACACCC 
ACGGATGCCG GTGGCGGAGT GGTGGCGGTG CAAAGTATAC TGAGATTCAG GGGTAAGGCT 
AGACCGAATG GAAACCAACT ACTTCCGAAC GATAGAGGTC TGTATACTTG CCTTTACGAA 
AACGACGTAA ATTCGGCCAA TTCTTCAATG CATCTGAGGA TCGAGCACGA ACCGATAGTC 
ATTCATCAGT ACAACAAAGT GGCCTACGAT CTTCGAGAAT CCGCTGAGGT TGTCTGCAGG 
GTTCAGGCCT ATCCGAAACC GGAGTTTCAA TGGCAATACG GCAACAATCC TTCGCCATTG 
ACCATGTCTT CGGATGGTCA TTATGAAATT AGTACGAGAA TGGAGAATAA TGATGTGTAC 
ACCTCCATTT TGAGGATAGC ACACTTGCAG CACTCTGATT ACGGAGAATA TATTTGCAGA 
GCTGTAAATC CTTTGGACAG CATAAGAGCA CCAATAAGAC TGCAACCCAA AGGATCGCCA 
GAGAAACCCA CCAATTTAAA GATCCTCGAA GTTGGTCACA ACTATGCCGT CCTAAACTGG 
ACACCGGGAT TTAATGGCGG CTTTATGAGC ACCAAATATC TGGTGAGCTA CAGGAGAGTG 
GCAACACCCA GGGAACAAAC CCTATCCGAT TGTTCAGGAA ATGGCTATAT ACCCAGCTAT 
CAGATCAGCA GTTCGTCCAG TAATTCCAAC CACGAATGGA TCGAATTCAA TTGCTTTAAG 
GAGAACCCCT GTAAACTCGC ACCATTGGAT CAACATCAGA GTTATATGTT TAAGGTTTAT 
GCCCTGAATT CGAAGGGCAC CTCGGGATAT TCGAATGAGA TATTGGCCAC CACCAAAGTC 
AGTAAAATTC CACCGCCTTT GCATGTTAGC TACGATCCCA ATTCACATGT TCTGGGCATC 
AACGTGGCCG CCACCTGTCT GTCCCTCATT GCGGTGGTGG AATCACTGGT AACCAGGGAT 
GCCACGGTGC CCATGTGGGA GATTGTGGAA ACCCTGACTT TGCTCCCGTC GGGCAGCGAA 
ACGACCTTCA AGGAGGCCAT CATCAATCAT GTGTCGCGTC CAGCCCACTA CACCACCGCC 
ACCACGTCCG GAAGGAGCCT TGGAGTGGGT GGTGGCTCCC ACCTGGGCGA GGATCGCACC 
ATGGCGTTGG CCGAGACCGC GGGACCCGGT CCAGTGGTGC GGGTCAAACT CTGTCTGAGA 
TCTAACCACG AGCACTGTGG TGCATATGCC AATGCAGAAA TTGGCAAGTC CTACATGCCG 
CACAAATCCT CCATGACGAC ATCTGCATTA GTGGCCATCA TCATCGCTTC GCTTTCTTTC 
GTCGTCTTTC TTGGTCTTCT CTACGCTTTT TGCCACTGCC GTAGGAAACA TGCCGCCAAA 
AAGGAGAGTA GCTCAGTAGG AGGAGGAGTT GGAGGAGGCA ACGCTAACGC CACGGCTAAT 
CCAGGATCTA CCGGTGCCAA AGAGTACGAC CTCGACTTGG ACGCCAGCCG AAGACCGTCG 
CTAAGTCAGG ATCCACAGCA ATCGCAACAG CAGCCACCGC CACCACCACC CTACTATCCC 
ACGGGAACTC TGGACTCCAA GGACATTGGC AATGGGAACG GCGGCATGGA GCTCACCCTC 
ACCGCCCTAC ACGATCCTGA TGAGCAGCTG AATATGCAAC AGCAGCAGCA CCACAGCAAC 
CACGGCCAGT ATCAACAGCC CAAAGCCATA CTGGGTATCT ACGGCGGAGT GGCTGGCTCT 
GGGGGCAACA ATTCCGGCGG ACAGCATCCA CATTCGAACG GCTACGGCTA TCATGTGACA 
AGTGCCATCG GCGTGGACAG CGACAGCTAT CAAGTGCTGC CCTCCGTTGC CAACTCAGCA 
GCCGGATCCC ACGGACATGG CAGTGGACAC GGACACGGAC TAGGAGCCGG AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722954.3
CDS	547..4143
Translation	MSTIKLLIIGQLWLSIGLISGDDSLDTREGVDLVLKCRFTEHYDSTDFTFYWARWTCCPTLFENVAIGDVQLNSNYRLDFRPSRGIYDLQIKNTSYNRDNGRFECRIKAKGTGADVHQEFYNLTVLTAPHPPMVTPGNLAVATEEKPLELTCSSIGGSPDPMITWYREGSTVPLQSYALKGGSKNHYTNATLQIVPRRADDGAKYKCVVWNRAMPEGHMLETSVTLNVNYYPRVEVGPQNPLKVERDHVAKLDCRVDAKPMVSNVRWSRNGQYVSATPTHTIYRVNRHHAGKYTCSADNGLGKTGEKDIVLDVLYPPIVFIESKTHEAEEGETVLIRCNVTANPSPINVEWLKEGAPDFRYTGELLTLGSVRAEHAGNYICRSVNIMQPFSSKRVEGVGNSTVALLVRHRPGQAYITPNKPVVHVGNGVTLTCSANPPGWPVPQYRWFRDMDGDIGNTQKILAQGPQYSIPKAHLGSEGKYHCHAVNELGIGKIATIILEVHQPPQFLAKLQQHMTRRVGDVDYAVTCSAKGKPTPQIRWIKDGTEILPTRKMFDIRTTPTDAGGGVVAVQSILRFRGKARPNGNQLLPNDRGLYTCLYENDVNSANSSMHLRIEHEPIVIHQYNKVAYDLRESAEVVCRVQAYPKPEFQWQYGNNPSPLTMSSDGHYEISTRMENNDVYTSILRIAHLQHSDYGEYICRAVNPLDSIRAPIRLQPKGSPEKPTNLKILEVGHNYAVLNWTPGFNGGFMSTKYLVSYRRVATPREQTLSDCSGNGYIPSYQISSSSSNSNHEWIEFNCFKENPCKLAPLDQHQSYMFKVYALNSKGTSGYSNEILATTKVSKIPPPLHVSYDPNSHVLGINVAATCLSLIAVVESLVTRDATVPMWEIVETLTLLPSGSETTFKEAIINHVSRPAHYTTATTSGRSLGVGGGSHLGEDRTMALAETAGPGPVVRVKLCLRSNHEHCGAYANAEIGKSYMPHKSSMTTSALVAIIIASLSFVVFLGLLYAFCHCRRKHAAKKESSSVGGGVGGGNANATANPGSTGAKEYDLDLDASRRPSLSQDPQQSQQQPPPPPPYYPTGTLDSKDIGNGNGGMELTLTALHDPDEQLNMQQQQHHSNHGQYQQPKAILGIYGGVAGSGGNNSGGQHPHSNGYGYHVTSAIGVDSDSYQVLPSVANSAAGSHGHGSGHGHGLGAGE

Target ORF information:

RefSeq Version	NM_164569.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster friend of echinoid (fred), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164569.4

ORF Insert Sequence:

ATGTCGACCA TAAAGCTGCT CATTATCGGT CAACTTTGGC TCTCGATTGG CCTGATCTCC 
GGGGACGATT CGCTGGACAC GCGTGAGGGC GTCGATCTGG TGCTCAAGTG CCGCTTCACC 
GAGCATTACG ACTCGACCGA CTTCACCTTC TACTGGGCGC GATGGACCTG CTGCCCCACA 
TTGTTCGAGA ACGTGGCCAT TGGCGATGTG CAGCTCAACT CGAATTATCG CTTGGACTTC 
CGACCCAGTC GCGGAATTTA CGATTTGCAA ATCAAAAACA CATCTTATAA TCGAGATAAT 
GGACGCTTTG AGTGCCGCAT CAAGGCGAAG GGAACGGGCG CGGATGTCCA TCAGGAGTTC 
TACAATCTGA CCGTGCTGAC GGCTCCACAT CCGCCGATGG TCACTCCCGG CAATTTAGCA 
GTGGCCACCG AAGAGAAGCC ATTGGAGTTG ACCTGCAGCA GTATTGGTGG CTCACCAGAT 
CCAATGATAA CCTGGTACCG AGAGGGCAGC ACAGTGCCAC TGCAGTCGTA TGCTCTGAAG 
GGCGGATCGA AGAACCACTA TACAAACGCC ACACTCCAAA TTGTTCCCCG ACGGGCCGAC 
GATGGCGCCA AGTACAAATG CGTCGTCTGG AATCGTGCCA TGCCCGAGGG TCATATGCTG 
GAGACCAGCG TCACGCTGAA TGTCAACTAC TATCCCCGAG TGGAGGTCGG ACCCCAGAAT 
CCTTTAAAAG TAGAACGGGA TCATGTGGCG AAACTGGACT GCAGAGTGGA TGCCAAACCG 
ATGGTTAGCA ATGTGCGATG GTCCCGAAAT GGTCAATATG TGAGTGCCAC GCCCACCCAC 
ACCATATATA GAGTAAATCG CCATCATGCC GGGAAGTACA CCTGCAGTGC GGACAACGGC 
TTGGGCAAAA CTGGCGAAAA AGATATAGTC TTGGATGTTC TATATCCGCC GATTGTGTTT 
ATCGAATCGA AAACGCACGA AGCGGAGGAA GGTGAAACGG TTTTGATTCG TTGCAATGTC 
ACCGCAAATC CATCGCCCAT CAATGTCGAA TGGCTAAAAG AAGGAGCTCC GGACTTCCGA 
TACACTGGCG AGCTCTTAAC CCTCGGTTCA GTGAGGGCTG AGCACGCCGG AAACTATATC 
TGCAGATCGG TGAACATTAT GCAACCGTTC AGTTCGAAAC GTGTAGAGGG TGTGGGCAAT 
TCCACGGTGG CTCTATTGGT TCGACATCGG CCGGGCCAGG CTTATATAAC TCCGAATAAG 
CCAGTCGTTC ATGTGGGCAA TGGTGTGACT CTAACCTGTT CAGCGAATCC GCCCGGATGG 
CCTGTACCCC AATACCGATG GTTCCGAGAT ATGGATGGTG ACATTGGAAA TACACAGAAA 
ATTTTAGCGC AAGGTCCACA GTACTCTATA CCGAAAGCTC ACTTGGGAAG CGAGGGCAAG 
TACCACTGTC ATGCGGTCAA CGAGTTGGGA ATTGGTAAAA TAGCCACCAT AATCTTAGAG 
GTACATCAGC CACCCCAGTT TCTGGCCAAA CTACAGCAAC ATATGACACG TCGGGTGGGC 
GATGTAGACT ATGCGGTCAC CTGCAGTGCC AAGGGAAAGC CTACACCGCA AATTCGATGG 
ATCAAAGATG GTACCGAGAT TCTGCCCACT CGCAAGATGT TTGATATAAG AACTACACCC 
ACGGATGCCG GTGGCGGAGT GGTGGCGGTG CAAAGTATAC TGAGATTCAG GGGTAAGGCT 
AGACCGAATG GAAACCAACT ACTTCCGAAC GATAGAGGTC TGTATACTTG CCTTTACGAA 
AACGACGTAA ATTCGGCCAA TTCTTCAATG CATCTGAGGA TCGAGCACGA ACCGATAGTC 
ATTCATCAGT ACAACAAAGT GGCCTACGAT CTTCGAGAAT CCGCTGAGGT TGTCTGCAGG 
GTTCAGGCCT ATCCGAAACC GGAGTTTCAA TGGCAATACG GCAACAATCC TTCGCCATTG 
ACCATGTCTT CGGATGGTCA TTATGAAATT AGTACGAGAA TGGAGAATAA TGATGTGTAC 
ACCTCCATTT TGAGGATAGC ACACTTGCAG CACTCTGATT ACGGAGAATA TATTTGCAGA 
GCTGTAAATC CTTTGGACAG CATAAGAGCA CCAATAAGAC TGCAACCCAA AGGATCGCCA 
GAGAAACCCA CCAATTTAAA GATCCTCGAA GTTGGTCACA ACTATGCCGT CCTAAACTGG 
ACACCGGGAT TTAATGGCGG CTTTATGAGC ACCAAATATC TGGTGAGCTA CAGGAGAGTG 
GCAACACCCA GGGAACAAAC CCTATCCGAT TGTTCAGGAA ATGGCTATAT ACCCAGCTAT 
CAGATCAGCA GTTCGTCCAG TAATTCCAAC CACGAATGGA TCGAATTCAA TTGCTTTAAG 
GAGAACCCCT GTAAACTCGC ACCATTGGAT CAACATCAGA GTTATATGTT TAAGGTTTAT 
GCCCTGAATT CGAAGGGCAC CTCGGGATAT TCGAATGAGA TATTGGCCAC CACCAAAGTC 
AGTAAAATTC CACCGCCTTT GCATGTTAGC TACGATCCCA ATTCACATGT TCTGGGCATC 
AACGTGGCCG CCACCTGTCT GTCCCTCATT GCGGTGGTGG AATCACTGGT AACCAGGGAT 
GCCACGGTGC CCATGTGGGA GATTGTGGAA ACCCTGACTT TGCTCCCGTC GGGCAGCGAA 
ACGACCTTCA AGGAGGCCAT CATCAATCAT GTGTCGCGTC CAGCCCACTA CACCACCGCC 
ACCACGTCCG GAAGGAGCCT TGGAGTGGGT GGTGGCTCCC ACCTGGGCGA GGATCGCACC 
ATGGCGTTGG CCGAGACCGC GGGACCCGGT CCAGTGGTGC GGGTCAAACT CTGTCTGAGA 
TCTAACCACG AGCACTGTGG TGCATATGCC AATGCAGAAA TTGGCAAGTC CTACATGCCG 
CACAAATCCT CCATGACGAC ATCTGCATTA GTGGCCATCA TCATCGCTTC GCTTTCTTTC 
GTCGTCTTTC TTGGTCTTCT CTACGCTTTT TGCCACTGCC GTAGGAAACA TGCCGCCAAA 
AAGGAGAGTA GCTCAGTAGG AGGAGGAGTT GGAGGAGGCA ACGCTAACGC CACGGCTAAT 
CCAGGATCTA CCGGTGCCAA AGAGTACGAC CTCGACTTGG ACGCCAGCCG AAGACCGTCG 
CTAAGTCAGG ATCCACAGCA ATCGCAACAG CAGCCACCGC CACCACCACC CTACTATCCC 
ACGGGAACTC TGGACTCCAA GGACATTGGC AATGGGAACG GCGGCATGGA GCTCACCCTC 
ACCGCCCTAC ACGATCCTGA TGAGCAGCTG AATATGCAAC AGCAGCAGCA CCACAGCAAC 
CACGGCCAGT ATCAACAGCC CAAAGCCATA CTGGGTATCT ACGGCGGAGT GGCTGGCTCT 
GGGGGCAACA ATTCCGGCGG ACAGCATCCA CATTCGAACG GCTACGGCTA TCATGTGACA 
AGTGCCATCG GCGTGGACAG CGACAGCTAT CAAGTGCTGC CCTCCGTTGC CAACTCAGCA 
GCCGGATCCC ACGGACATGG CAGTGGACAC GGACACGGAC TAGGAGCCGG AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fred ( NM_001258937.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245866.1 Drosophila melanogaster friend of echinoid (fred), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa16205
Clone ID Related Accession (Same CDS sequence)	NM_001258937.2
Accession Version	NM_001258937.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	friend of echinoid, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001258937.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGACCA TAAAGCTGCT CATTATCGGT CAACTTTGGC TCTCGATTGG CCTGATCTCC 
GGGGACGATT CGCTGGACAC GCGTGAGGGC GTCGATCTGG TGCTCAAGTG CCGCTTCACC 
GAGCATTACG ACTCGACCGA CTTCACCTTC TACTGGGCGC GATGGACCTG CTGCCCCACA 
TTGTTCGAGA ACGTGGCCAT TGGCGATGTG CAGCTCAACT CGAATTATCG CTTGGACTTC 
CGACCCAGTC GCGGAATTTA CGATTTGCAA ATCAAAAACA CATCTTATAA TCGAGATAAT 
GGACGCTTTG AGTGCCGCAT CAAGGCGAAG GGAACGGGCG CGGATGTCCA TCAGGAGTTC 
TACAATCTGA CCGTGCTGAC GGCTCCACAT CCGCCGATGG TCACTCCCGG CAATTTAGCA 
GTGGCCACCG AAGAGAAGCC ATTGGAGTTG ACCTGCAGCA GTATTGGTGG CTCACCAGAT 
CCAATGATAA CCTGGTACCG AGAGGGCAGC ACAGTGCCAC TGCAGTCGTA TGCTCTGAAG 
GGCGGATCGA AGAACCACTA TACAAACGCC ACACTCCAAA TTGTTCCCCG ACGGGCCGAC 
GATGGCGCCA AGTACAAATG CGTCGTCTGG AATCGTGCCA TGCCCGAGGG TCATATGCTG 
GAGACCAGCG TCACGCTGAA TGTCAACTAC TATCCCCGAG TGGAGGTCGG ACCCCAGAAT 
CCTTTAAAAG TAGAACGGGA TCATGTGGCG AAACTGGACT GCAGAGTGGA TGCCAAACCG 
ATGGTTAGCA ATGTGCGATG GTCCCGAAAT GGTCAATATG TGAGTGCCAC GCCCACCCAC 
ACCATATATA GAGTAAATCG CCATCATGCC GGGAAGTACA CCTGCAGTGC GGACAACGGC 
TTGGGCAAAA CTGGCGAAAA AGATATAGTC TTGGATGTTC TATATCCGCC GATTGTGTTT 
ATCGAATCGA AAACGCACGA AGCGGAGGAA GGTGAAACGG TTTTGATTCG TTGCAATGTC 
ACCGCAAATC CATCGCCCAT CAATGTCGAA TGGCTAAAAG AAGGAGCTCC GGACTTCCGA 
TACACTGGCG AGCTCTTAAC CCTCGGTTCA GTGAGGGCTG AGCACGCCGG AAACTATATC 
TGCAGATCGG TGAACATTAT GCAACCGTTC AGTTCGAAAC GTGTAGAGGG TGTGGGCAAT 
TCCACGGTGG CTCTATTGGT TCGACATCGG CCGGGCCAGG CTTATATAAC TCCGAATAAG 
CCAGTCGTTC ATGTGGGCAA TGGTGTGACT CTAACCTGTT CAGCGAATCC GCCCGGATGG 
CCTGTACCCC AATACCGATG GTTCCGAGAT ATGGATGGTG ACATTGGAAA TACACAGAAA 
ATTTTAGCGC AAGGTCCACA GTACTCTATA CCGAAAGCTC ACTTGGGAAG CGAGGGCAAG 
TACCACTGTC ATGCGGTCAA CGAGTTGGGA ATTGGTAAAA TAGCCACCAT AATCTTAGAG 
GTACATCAGC CACCCCAGTT TCTGGCCAAA CTACAGCAAC ATATGACACG TCGGGTGGGC 
GATGTAGACT ATGCGGTCAC CTGCAGTGCC AAGGGAAAGC CTACACCGCA AATTCGATGG 
ATCAAAGATG GTACCGAGAT TCTGCCCACT CGCAAGATGT TTGATATAAG AACTACACCC 
ACGGATGCCG GTGGCGGAGT GGTGGCGGTG CAAAGTATAC TGAGATTCAG GGGTAAGGCT 
AGACCGAATG GAAACCAACT ACTTCCGAAC GATAGAGGTC TGTATACTTG CCTTTACGAA 
AACGACGTAA ATTCGGCCAA TTCTTCAATG CATCTGAGGA TCGAGCACGA ACCGATAGTC 
ATTCATCAGT ACAACAAAGT GGCCTACGAT CTTCGAGAAT CCGCTGAGGT TGTCTGCAGG 
GTTCAGGCCT ATCCGAAACC GGAGTTTCAA TGGCAATACG GCAACAATCC TTCGCCATTG 
ACCATGTCTT CGGATGGTCA TTATGAAATT AGTACGAGAA TGGAGAATAA TGATGTGTAC 
ACCTCCATTT TGAGGATAGC ACACTTGCAG CACTCTGATT ACGGAGAATA TATTTGCAGA 
GCTGTAAATC CTTTGGACAG CATAAGAGCA CCAATAAGAC TGCAACCCAA AGGATCGCCA 
GAGAAACCCA CCAATTTAAA GATCCTCGAA GTTGGTCACA ACTATGCCGT CCTAAACTGG 
ACACCGGGAT TTAATGGCGG CTTTATGAGC ACCAAATATC TGGTGAGCTA CAGGAGAGTG 
GCAACACCCA GGGAACAAAC CCTATCCGAT TGTTCAGGAA ATGGCTATAT ACCCAGCTAT 
CAGATCAGCA GTTCGTCCAG TAATTCCAAC CACGAATGGA TCGAATTCAA TTGCTTTAAG 
GAGAACCCCT GTAAACTCGC ACCATTGGAT CAACATCAGA GTTATATGTT TAAGGTTTAT 
GCCCTGAATT CGAAGGGCAC CTCGGGATAT TCGAATGAGA TATTGGCCAC CACCAAAGTC 
AGTAAAATTC CACCGCCTTT GCATGTTAGC TACGATCCCA ATTCACATGT TCTGGGCATC 
AACGTGGCCG CCACCTGTCT GTCCCTCATT GCGGTGGTGG AATCACTGGT AACCAGGGAT 
GCCACGGTGC CCATGTGGGA GATTGTGGAA ACCCTGACTT TGCTCCCGTC GGGCAGCGAA 
ACGACCTTCA AGGAGGCCAT CATCAATCAT GTGTCGCGTC CAGCCCACTA CACCACCGCC 
ACCACGTCCG GAAGGAGCCT TGGAGTGGGT GGTGGCTCCC ACCTGGGCGA GGATCGCACC 
ATGGCGTTGG CCGAGACCGC GGGACCCGGT CCAGTGGTGC GGGTCAAACT CTGTCTGAGA 
TCTAACCACG AGCACTGTGG TGCATATGCC AATGCAGAAA TTGGCAAGTC CTACATGCCG 
CACAAATCCT CCATGACGAC ATCTGCATTA GTGGCCATCA TCATCGCTTC GCTTTCTTTC 
GTCGTCTTTC TTGGTCTTCT CTACGCTTTT TGCCACTGCC GTAGGAAACA TGCCGCCAAA 
AAGGAGAGTA GCTCAGTAGG AGGAGGAGTT GGAGGAGGCA ACGCTAACGC CACGGCTAAT 
CCAGGATCTA CCGGTGCCAA AGAGTACGAC CTCGACTTGG ACGCCAGCCG AAGACCGTCG 
CTAAGTCAGG ATCCACAGCA ATCGCAACAG CAGCCACCGC CACCACCACC CTACTATCCC 
ACGGGAACTC TGGACTCCAA GGACATTGGC AATGGGAACG GCGGCATGGA GCTCACCCTC 
ACCGCCCTAC ACGATCCTGA TGAGCAGCTG AATATGCAAC AGCAGCAGCA CCACAGCAAC 
CACGGCCAGT ATCAACAGCC CAAAGCCATA CTGGGTATCT ACGGCGGAGT GGCTGGCTCT 
GGGGGCAACA ATTCCGGCGG ACAGCATCCA CATTCGAACG GCTACGGCTA TCATGTGACA 
AGTGCCATCG GCGTGGACAG CGACAGCTAT CAAGTGCTGC CCTCCGTTGC CAACTCAGCA 
GCCGGATCCC ACGGACATGG CAGTGGACAC GGACACGGAC TAGGAGCCGG AGAGTGGTCA 
AGCAGCAATT CGAACGAGAA TAACTACAGT AATGCCAGGG ATCTGTCGCA GGAGGCATTG 
TGGCGCCTCC AAATGGCCAC CGCCCAGTCG CAACAGATTT ACGTGGAGCG ACCACCGTCG 
GCATTCAGTG GCCTGGTCGA CTATAGTGGC TATTCGCCGC ACATCCCCAC GGTGACCAGT 
TCGCTCAGCC AACAGAGTTT CAGTCCAACG CAGCAATTAG CGCCCCACGA AATGCTGCAG 
GCCGCCCAGC GTTACGGAAC TTTGAGGAAA TCCGGAAAAC AACCGCCTCT TCCCCCGCAG 
CGAAAGGATA TGCAGCAGCA GGCGAAGCCA CCTCAGCAAA TGGCGGATAT AATACAGGAT 
TTGGCAAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245866.1
CDS	547..4518
Translation	MSTIKLLIIGQLWLSIGLISGDDSLDTREGVDLVLKCRFTEHYDSTDFTFYWARWTCCPTLFENVAIGDVQLNSNYRLDFRPSRGIYDLQIKNTSYNRDNGRFECRIKAKGTGADVHQEFYNLTVLTAPHPPMVTPGNLAVATEEKPLELTCSSIGGSPDPMITWYREGSTVPLQSYALKGGSKNHYTNATLQIVPRRADDGAKYKCVVWNRAMPEGHMLETSVTLNVNYYPRVEVGPQNPLKVERDHVAKLDCRVDAKPMVSNVRWSRNGQYVSATPTHTIYRVNRHHAGKYTCSADNGLGKTGEKDIVLDVLYPPIVFIESKTHEAEEGETVLIRCNVTANPSPINVEWLKEGAPDFRYTGELLTLGSVRAEHAGNYICRSVNIMQPFSSKRVEGVGNSTVALLVRHRPGQAYITPNKPVVHVGNGVTLTCSANPPGWPVPQYRWFRDMDGDIGNTQKILAQGPQYSIPKAHLGSEGKYHCHAVNELGIGKIATIILEVHQPPQFLAKLQQHMTRRVGDVDYAVTCSAKGKPTPQIRWIKDGTEILPTRKMFDIRTTPTDAGGGVVAVQSILRFRGKARPNGNQLLPNDRGLYTCLYENDVNSANSSMHLRIEHEPIVIHQYNKVAYDLRESAEVVCRVQAYPKPEFQWQYGNNPSPLTMSSDGHYEISTRMENNDVYTSILRIAHLQHSDYGEYICRAVNPLDSIRAPIRLQPKGSPEKPTNLKILEVGHNYAVLNWTPGFNGGFMSTKYLVSYRRVATPREQTLSDCSGNGYIPSYQISSSSSNSNHEWIEFNCFKENPCKLAPLDQHQSYMFKVYALNSKGTSGYSNEILATTKVSKIPPPLHVSYDPNSHVLGINVAATCLSLIAVVESLVTRDATVPMWEIVETLTLLPSGSETTFKEAIINHVSRPAHYTTATTSGRSLGVGGGSHLGEDRTMALAETAGPGPVVRVKLCLRSNHEHCGAYANAEIGKSYMPHKSSMTTSALVAIIIASLSFVVFLGLLYAFCHCRRKHAAKKESSSVGGGVGGGNANATANPGSTGAKEYDLDLDASRRPSLSQDPQQSQQQPPPPPPYYPTGTLDSKDIGNGNGGMELTLTALHDPDEQLNMQQQQHHSNHGQYQQPKAILGIYGGVAGSGGNNSGGQHPHSNGYGYHVTSAIGVDSDSYQVLPSVANSAAGSHGHGSGHGHGLGAGEWSSSNSNENNYSNARDLSQEALWRLQMATAQSQQIYVERPPSAFSGLVDYSGYSPHIPTVTSSLSQQSFSPTQQLAPHEMLQAAQRYGTLRKSGKQPPLPPQRKDMQQQAKPPQQMADIIQDLAN

Target ORF information:

RefSeq Version	NM_001258937.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster friend of echinoid (fred), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258937.2

ORF Insert Sequence:

ATGTCGACCA TAAAGCTGCT CATTATCGGT CAACTTTGGC TCTCGATTGG CCTGATCTCC 
GGGGACGATT CGCTGGACAC GCGTGAGGGC GTCGATCTGG TGCTCAAGTG CCGCTTCACC 
GAGCATTACG ACTCGACCGA CTTCACCTTC TACTGGGCGC GATGGACCTG CTGCCCCACA 
TTGTTCGAGA ACGTGGCCAT TGGCGATGTG CAGCTCAACT CGAATTATCG CTTGGACTTC 
CGACCCAGTC GCGGAATTTA CGATTTGCAA ATCAAAAACA CATCTTATAA TCGAGATAAT 
GGACGCTTTG AGTGCCGCAT CAAGGCGAAG GGAACGGGCG CGGATGTCCA TCAGGAGTTC 
TACAATCTGA CCGTGCTGAC GGCTCCACAT CCGCCGATGG TCACTCCCGG CAATTTAGCA 
GTGGCCACCG AAGAGAAGCC ATTGGAGTTG ACCTGCAGCA GTATTGGTGG CTCACCAGAT 
CCAATGATAA CCTGGTACCG AGAGGGCAGC ACAGTGCCAC TGCAGTCGTA TGCTCTGAAG 
GGCGGATCGA AGAACCACTA TACAAACGCC ACACTCCAAA TTGTTCCCCG ACGGGCCGAC 
GATGGCGCCA AGTACAAATG CGTCGTCTGG AATCGTGCCA TGCCCGAGGG TCATATGCTG 
GAGACCAGCG TCACGCTGAA TGTCAACTAC TATCCCCGAG TGGAGGTCGG ACCCCAGAAT 
CCTTTAAAAG TAGAACGGGA TCATGTGGCG AAACTGGACT GCAGAGTGGA TGCCAAACCG 
ATGGTTAGCA ATGTGCGATG GTCCCGAAAT GGTCAATATG TGAGTGCCAC GCCCACCCAC 
ACCATATATA GAGTAAATCG CCATCATGCC GGGAAGTACA CCTGCAGTGC GGACAACGGC 
TTGGGCAAAA CTGGCGAAAA AGATATAGTC TTGGATGTTC TATATCCGCC GATTGTGTTT 
ATCGAATCGA AAACGCACGA AGCGGAGGAA GGTGAAACGG TTTTGATTCG TTGCAATGTC 
ACCGCAAATC CATCGCCCAT CAATGTCGAA TGGCTAAAAG AAGGAGCTCC GGACTTCCGA 
TACACTGGCG AGCTCTTAAC CCTCGGTTCA GTGAGGGCTG AGCACGCCGG AAACTATATC 
TGCAGATCGG TGAACATTAT GCAACCGTTC AGTTCGAAAC GTGTAGAGGG TGTGGGCAAT 
TCCACGGTGG CTCTATTGGT TCGACATCGG CCGGGCCAGG CTTATATAAC TCCGAATAAG 
CCAGTCGTTC ATGTGGGCAA TGGTGTGACT CTAACCTGTT CAGCGAATCC GCCCGGATGG 
CCTGTACCCC AATACCGATG GTTCCGAGAT ATGGATGGTG ACATTGGAAA TACACAGAAA 
ATTTTAGCGC AAGGTCCACA GTACTCTATA CCGAAAGCTC ACTTGGGAAG CGAGGGCAAG 
TACCACTGTC ATGCGGTCAA CGAGTTGGGA ATTGGTAAAA TAGCCACCAT AATCTTAGAG 
GTACATCAGC CACCCCAGTT TCTGGCCAAA CTACAGCAAC ATATGACACG TCGGGTGGGC 
GATGTAGACT ATGCGGTCAC CTGCAGTGCC AAGGGAAAGC CTACACCGCA AATTCGATGG 
ATCAAAGATG GTACCGAGAT TCTGCCCACT CGCAAGATGT TTGATATAAG AACTACACCC 
ACGGATGCCG GTGGCGGAGT GGTGGCGGTG CAAAGTATAC TGAGATTCAG GGGTAAGGCT 
AGACCGAATG GAAACCAACT ACTTCCGAAC GATAGAGGTC TGTATACTTG CCTTTACGAA 
AACGACGTAA ATTCGGCCAA TTCTTCAATG CATCTGAGGA TCGAGCACGA ACCGATAGTC 
ATTCATCAGT ACAACAAAGT GGCCTACGAT CTTCGAGAAT CCGCTGAGGT TGTCTGCAGG 
GTTCAGGCCT ATCCGAAACC GGAGTTTCAA TGGCAATACG GCAACAATCC TTCGCCATTG 
ACCATGTCTT CGGATGGTCA TTATGAAATT AGTACGAGAA TGGAGAATAA TGATGTGTAC 
ACCTCCATTT TGAGGATAGC ACACTTGCAG CACTCTGATT ACGGAGAATA TATTTGCAGA 
GCTGTAAATC CTTTGGACAG CATAAGAGCA CCAATAAGAC TGCAACCCAA AGGATCGCCA 
GAGAAACCCA CCAATTTAAA GATCCTCGAA GTTGGTCACA ACTATGCCGT CCTAAACTGG 
ACACCGGGAT TTAATGGCGG CTTTATGAGC ACCAAATATC TGGTGAGCTA CAGGAGAGTG 
GCAACACCCA GGGAACAAAC CCTATCCGAT TGTTCAGGAA ATGGCTATAT ACCCAGCTAT 
CAGATCAGCA GTTCGTCCAG TAATTCCAAC CACGAATGGA TCGAATTCAA TTGCTTTAAG 
GAGAACCCCT GTAAACTCGC ACCATTGGAT CAACATCAGA GTTATATGTT TAAGGTTTAT 
GCCCTGAATT CGAAGGGCAC CTCGGGATAT TCGAATGAGA TATTGGCCAC CACCAAAGTC 
AGTAAAATTC CACCGCCTTT GCATGTTAGC TACGATCCCA ATTCACATGT TCTGGGCATC 
AACGTGGCCG CCACCTGTCT GTCCCTCATT GCGGTGGTGG AATCACTGGT AACCAGGGAT 
GCCACGGTGC CCATGTGGGA GATTGTGGAA ACCCTGACTT TGCTCCCGTC GGGCAGCGAA 
ACGACCTTCA AGGAGGCCAT CATCAATCAT GTGTCGCGTC CAGCCCACTA CACCACCGCC 
ACCACGTCCG GAAGGAGCCT TGGAGTGGGT GGTGGCTCCC ACCTGGGCGA GGATCGCACC 
ATGGCGTTGG CCGAGACCGC GGGACCCGGT CCAGTGGTGC GGGTCAAACT CTGTCTGAGA 
TCTAACCACG AGCACTGTGG TGCATATGCC AATGCAGAAA TTGGCAAGTC CTACATGCCG 
CACAAATCCT CCATGACGAC ATCTGCATTA GTGGCCATCA TCATCGCTTC GCTTTCTTTC 
GTCGTCTTTC TTGGTCTTCT CTACGCTTTT TGCCACTGCC GTAGGAAACA TGCCGCCAAA 
AAGGAGAGTA GCTCAGTAGG AGGAGGAGTT GGAGGAGGCA ACGCTAACGC CACGGCTAAT 
CCAGGATCTA CCGGTGCCAA AGAGTACGAC CTCGACTTGG ACGCCAGCCG AAGACCGTCG 
CTAAGTCAGG ATCCACAGCA ATCGCAACAG CAGCCACCGC CACCACCACC CTACTATCCC 
ACGGGAACTC TGGACTCCAA GGACATTGGC AATGGGAACG GCGGCATGGA GCTCACCCTC 
ACCGCCCTAC ACGATCCTGA TGAGCAGCTG AATATGCAAC AGCAGCAGCA CCACAGCAAC 
CACGGCCAGT ATCAACAGCC CAAAGCCATA CTGGGTATCT ACGGCGGAGT GGCTGGCTCT 
GGGGGCAACA ATTCCGGCGG ACAGCATCCA CATTCGAACG GCTACGGCTA TCATGTGACA 
AGTGCCATCG GCGTGGACAG CGACAGCTAT CAAGTGCTGC CCTCCGTTGC CAACTCAGCA 
GCCGGATCCC ACGGACATGG CAGTGGACAC GGACACGGAC TAGGAGCCGG AGAGTGGTCA 
AGCAGCAATT CGAACGAGAA TAACTACAGT AATGCCAGGG ATCTGTCGCA GGAGGCATTG 
TGGCGCCTCC AAATGGCCAC CGCCCAGTCG CAACAGATTT ACGTGGAGCG ACCACCGTCG 
GCATTCAGTG GCCTGGTCGA CTATAGTGGC TATTCGCCGC ACATCCCCAC GGTGACCAGT 
TCGCTCAGCC AACAGAGTTT CAGTCCAACG CAGCAATTAG CGCCCCACGA AATGCTGCAG 
GCCGCCCAGC GTTACGGAAC TTTGAGGAAA TCCGGAAAAC AACCGCCTCT TCCCCCGCAG 
CGAAAGGATA TGCAGCAGCA GGCGAAGCCA CCTCAGCAAA TGGCGGATAT AATACAGGAT 
TTGGCAAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fred ( NM_134968.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608812.3 Drosophila melanogaster friend of echinoid (fred), transcript variant H, mRNA.

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CloneID	OFa21586
Clone ID Related Accession (Same CDS sequence)	NM_134968.4
Accession Version	NM_134968.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	friend of echinoid, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_134968.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGACCA TAAAGCTGCT CATTATCGGT CAACTTTGGC TCTCGATTGG CCTGATCTCC 
GGGGACGATT CGCTGGACAC GCGTGAGGGC GTCGATCTGG TGCTCAAGTG CCGCTTCACC 
GAGCATTACG ACTCGACCGA CTTCACCTTC TACTGGGCGC GATGGACCTG CTGCCCCACA 
TTGTTCGAGA ACGTGGCCAT TGGCGATGTG CAGCTCAACT CGAATTATCG CTTGGACTTC 
CGACCCAGTC GCGGAATTTA CGATTTGCAA ATCAAAAACA CATCTTATAA TCGAGATAAT 
GGACGCTTTG AGTGCCGCAT CAAGGCGAAG GGAACGGGCG CGGATGTCCA TCAGGAGTTC 
TACAATCTGA CCGTGCTGAC GGCTCCACAT CCGCCGATGG TCACTCCCGG CAATTTAGCA 
GTGGCCACCG AAGAGAAGCC ATTGGAGTTG ACCTGCAGCA GTATTGGTGG CTCACCAGAT 
CCAATGATAA CCTGGTACCG AGAGGGCAGC ACAGTGCCAC TGCAGTCGTA TGCTCTGAAG 
GGCGGATCGA AGAACCACTA TACAAACGCC ACACTCCAAA TTGTTCCCCG ACGGGCCGAC 
GATGGCGCCA AGTACAAATG CGTCGTCTGG AATCGTGCCA TGCCCGAGGG TCATATGCTG 
GAGACCAGCG TCACGCTGAA TGTCAACTAC TATCCCCGAG TGGAGGTCGG ACCCCAGAAT 
CCTTTAAAAG TAGAACGGGA TCATGTGGCG AAACTGGACT GCAGAGTGGA TGCCAAACCG 
ATGGTTAGCA ATGTGCGATG GTCCCGAAAT GGTCAATATG TGAGTGCCAC GCCCACCCAC 
ACCATATATA GAGTAAATCG CCATCATGCC GGGAAGTACA CCTGCAGTGC GGACAACGGC 
TTGGGCAAAA CTGGCGAAAA AGATATAGTC TTGGATGTTC TATATCCGCC GATTGTGTTT 
ATCGAATCGA AAACGCACGA AGCGGAGGAA GGTGAAACGG TTTTGATTCG TTGCAATGTC 
ACCGCAAATC CATCGCCCAT CAATGTCGAA TGGCTAAAAG AAGGAGCTCC GGACTTCCGA 
TACACTGGCG AGCTCTTAAC CCTCGGTTCA GTGAGGGCTG AGCACGCCGG AAACTATATC 
TGCAGATCGG TGAACATTAT GCAACCGTTC AGTTCGAAAC GTGTAGAGGG TGTGGGCAAT 
TCCACGGTGG CTCTATTGGT TCGACATCGG CCGGGCCAGG CTTATATAAC TCCGAATAAG 
CCAGTCGTTC ATGTGGGCAA TGGTGTGACT CTAACCTGTT CAGCGAATCC GCCCGGATGG 
CCTGTACCCC AATACCGATG GTTCCGAGAT ATGGATGGTG ACATTGGAAA TACACAGAAA 
ATTTTAGCGC AAGGTCCACA GTACTCTATA CCGAAAGCTC ACTTGGGAAG CGAGGGCAAG 
TACCACTGTC ATGCGGTCAA CGAGTTGGGA ATTGGTAAAA TAGCCACCAT AATCTTAGAG 
GTACATCAGC CACCCCAGTT TCTGGCCAAA CTACAGCAAC ATATGACACG TCGGGTGGGC 
GATGTAGACT ATGCGGTCAC CTGCAGTGCC AAGGGAAAGC CTACACCGCA AATTCGATGG 
ATCAAAGATG GTACCGAGAT TCTGCCCACT CGCAAGATGT TTGATATAAG AACTACACCC 
ACGGATGCCG GTGGCGGAGT GGTGGCGGTG CAAAGTATAC TGAGATTCAG GGGTAAGGCT 
AGACCGAATG GAAACCAACT ACTTCCGAAC GATAGAGGTC TGTATACTTG CCTTTACGAA 
AACGACGTAA ATTCGGCCAA TTCTTCAATG CATCTGAGGA TCGAGCACGA ACCGATAGTC 
ATTCATCAGT ACAACAAAGT GGCCTACGAT CTTCGAGAAT CCGCTGAGGT TGTCTGCAGG 
GTTCAGGCCT ATCCGAAACC GGAGTTTCAA TGGCAATACG GCAACAATCC TTCGCCATTG 
ACCATGTCTT CGGATGGTCA TTATGAAATT AGTACGAGAA TGGAGAATAA TGATGTGTAC 
ACCTCCATTT TGAGGATAGC ACACTTGCAG CACTCTGATT ACGGAGAATA TATTTGCAGA 
GCTGTAAATC CTTTGGACAG CATAAGAGCA CCAATAAGAC TGCAACCCAA AGGATCGCCA 
GAGAAACCCA CCAATTTAAA GATCCTCGAA GTTGGTCACA ACTATGCCGT CCTAAACTGG 
ACACCGGGAT TTAATGGCGG CTTTATGAGC ACCAAATATC TGGTGAGCTA CAGGAGAGTG 
GCAACACCCA GGGAACAAAC CCTATCCGAT TGTTCAGGAA ATGGCTATAT ACCCAGCTAT 
CAGATCAGCA GTTCGTCCAG TAATTCCAAC CACGAATGGA TCGAATTCAA TTGCTTTAAG 
GAGAACCCCT GTAAACTCGC ACCATTGGAT CAACATCAGA GTTATATGTT TAAGGTTTAT 
GCCCTGAATT CGAAGGGCAC CTCGGGATAT TCGAATGAGA TATTGGCCAC CACCAAAGTC 
AGTAAAATTC CACCGCCTTT GCATGTTAGC TACGATCCCA ATTCACATGT TCTGGGCATC 
AACGTGGCCG CCACCTGTCT GTCCCTCATT GCGGTGGTGG AATCACTGGT AACCAGGGAT 
GCCACGGTGC CCATGTGGGA GATTGTGGAA ACCCTGACTT TGCTCCCGTC GGGCAGCGAA 
ACGACCTTCA AGGAGGCCAT CATCAATCAT GTGTCGCGTC CAGCCCACTA CACCACCGCC 
ACCACGTCCG GAAGGAGCCT TGGAGTGGGT GGTGGCTCCC ACCTGGGCGA GGATCGCACC 
ATGGCGTTGG CCGAGACCGC GGGACCCGGT CCAGTGGTGC GGGTCAAACT CTGTCTGAGA 
TCTAACCACG AGCACTGTGG TGCATATGCC AATGCAGAAA TTGGCAAGTC CTACATGCCG 
CACAAATCCT CCATGACGAC ATCTGCATTA GTGGCCATCA TCATCGCTTC GCTTTCTTTC 
GTCGTCTTTC TTGGTCTTCT CTACGCTTTT TGCCACTGCC GTAGGAAACA TGCCGCCAAA 
AAGGAGAGTA GCTCAGTAGG AGGAGGAGTT GGAGGAGGCA ACGCTAACGC CACGGCTAAT 
CCAGGATCTA CCGGTGCCAA AGAGTACGAC CTCGACTTGG ACGCCAGCCG AAGACCGTCG 
CTAAGTCAGG ATCCACAGCA ATCGCAACAG CAGCCACCGC CACCACCACC CTACTATCCC 
ACGGGAACTC TGGACTCCAA GGACATTGGC AATGGGAACG GCGGCATGGA GCTCACCCTC 
ACCGCCCTAC ACGATCCTGA TGAGCAGCTG AATATGCAAC AGCAGCAGCA CCACAGCAAC 
CACGGCCAGT ATCAACAGCC CAAAGCCATA CTGGGTATCT ACGGCGGAGT GGCTGGCTCT 
GGGGGCAACA ATTCCGGCGG ACAGCATCCA CATTCGAACG GCTACGGCTA TCATGTGACA 
AGTGCCATCG GCGTGGACAG CGACAGCTAT CAAGTGCTGC CCTCCGTTGC CAACTCAGCA 
GCCGGATCCC ACGGACATGG CAGTGGACAC GGACACGGAC TAGGAGCCGG AGAGTCAGGA 
CCACTTGAGG CGACACCACC AACATGCAAC ATCAGCGGCG GCAGCAGCAG CAACAGCGGC 
ATTAACCCAA TGCAGCAGCA ACATTCAGCA CGCGCCAATC TAACGAACCA ACCCACCATC 
GCCACTGCCA GCTCCACCAA CAACTACAAC AACCACTTGA ACAACACCAA CATCGCCCAC 
ACCACCAACA ACACCAACAA CTGCACCACC CTGAAACGCG GCCACTTGGG AAATAGGGAG 
AGAGAGCGGG AAAGATGCCA GGTAACCGCA GCCACGGCGG CAACAACAAC GGCACTGGCA 
ACCACCATAA CAACAACATC TCGCAATGCC AAGGCAGCAA CAACAACAAC AACACTAGCC 
ATTACAGGGT CAAGCAGCAA TTCGAACGAG AATAACTACA GTAATGCCAG GGATCTGTCG 
CAGGAGGCAT TGTGGCGCCT CCAAATGGCC ACCGCCCAGT CGCAACAGAT TTACGTGGAG 
CGACCACCGT CGGCATTCAG TGGCCTGGTC GACTATAGTG GCTATTCGCC GCACATCCCC 
ACGGTGACCA GTTCGCTCAG CCAACAGAGT TTCAGTCCAA CGCAGCAATT AGCGCCCCAC 
GAAATGCTGC AGGCCGCCCA GCGTTACGGA ACTTTGAGGA AATCCGGAAA ACAACCGCCT 
CTTCCCCCGC AGCGAAAGGA TATGCAGCAG CAGGCGAAGC CACCTCAGCA AATGGCGGAT 
ATAATACAGG ATTTGGCAAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608812.3
CDS	547..4890
Translation	MSTIKLLIIGQLWLSIGLISGDDSLDTREGVDLVLKCRFTEHYDSTDFTFYWARWTCCPTLFENVAIGDVQLNSNYRLDFRPSRGIYDLQIKNTSYNRDNGRFECRIKAKGTGADVHQEFYNLTVLTAPHPPMVTPGNLAVATEEKPLELTCSSIGGSPDPMITWYREGSTVPLQSYALKGGSKNHYTNATLQIVPRRADDGAKYKCVVWNRAMPEGHMLETSVTLNVNYYPRVEVGPQNPLKVERDHVAKLDCRVDAKPMVSNVRWSRNGQYVSATPTHTIYRVNRHHAGKYTCSADNGLGKTGEKDIVLDVLYPPIVFIESKTHEAEEGETVLIRCNVTANPSPINVEWLKEGAPDFRYTGELLTLGSVRAEHAGNYICRSVNIMQPFSSKRVEGVGNSTVALLVRHRPGQAYITPNKPVVHVGNGVTLTCSANPPGWPVPQYRWFRDMDGDIGNTQKILAQGPQYSIPKAHLGSEGKYHCHAVNELGIGKIATIILEVHQPPQFLAKLQQHMTRRVGDVDYAVTCSAKGKPTPQIRWIKDGTEILPTRKMFDIRTTPTDAGGGVVAVQSILRFRGKARPNGNQLLPNDRGLYTCLYENDVNSANSSMHLRIEHEPIVIHQYNKVAYDLRESAEVVCRVQAYPKPEFQWQYGNNPSPLTMSSDGHYEISTRMENNDVYTSILRIAHLQHSDYGEYICRAVNPLDSIRAPIRLQPKGSPEKPTNLKILEVGHNYAVLNWTPGFNGGFMSTKYLVSYRRVATPREQTLSDCSGNGYIPSYQISSSSSNSNHEWIEFNCFKENPCKLAPLDQHQSYMFKVYALNSKGTSGYSNEILATTKVSKIPPPLHVSYDPNSHVLGINVAATCLSLIAVVESLVTRDATVPMWEIVETLTLLPSGSETTFKEAIINHVSRPAHYTTATTSGRSLGVGGGSHLGEDRTMALAETAGPGPVVRVKLCLRSNHEHCGAYANAEIGKSYMPHKSSMTTSALVAIIIASLSFVVFLGLLYAFCHCRRKHAAKKESSSVGGGVGGGNANATANPGSTGAKEYDLDLDASRRPSLSQDPQQSQQQPPPPPPYYPTGTLDSKDIGNGNGGMELTLTALHDPDEQLNMQQQQHHSNHGQYQQPKAILGIYGGVAGSGGNNSGGQHPHSNGYGYHVTSAIGVDSDSYQVLPSVANSAAGSHGHGSGHGHGLGAGESGPLEATPPTCNISGGSSSNSGINPMQQQHSARANLTNQPTIATASSTNNYNNHLNNTNIAHTTNNTNNCTTLKRGHLGNRERERERCQVTAATAATTTALATTITTTSRNAKAATTTTTLAITGSSSNSNENNYSNARDLSQEALWRLQMATAQSQQIYVERPPSAFSGLVDYSGYSPHIPTVTSSLSQQSFSPTQQLAPHEMLQAAQRYGTLRKSGKQPPLPPQRKDMQQQAKPPQQMADIIQDLAN

Target ORF information:

RefSeq Version	NM_134968.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster friend of echinoid (fred), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134968.4

ORF Insert Sequence:

ATGTCGACCA TAAAGCTGCT CATTATCGGT CAACTTTGGC TCTCGATTGG CCTGATCTCC 
GGGGACGATT CGCTGGACAC GCGTGAGGGC GTCGATCTGG TGCTCAAGTG CCGCTTCACC 
GAGCATTACG ACTCGACCGA CTTCACCTTC TACTGGGCGC GATGGACCTG CTGCCCCACA 
TTGTTCGAGA ACGTGGCCAT TGGCGATGTG CAGCTCAACT CGAATTATCG CTTGGACTTC 
CGACCCAGTC GCGGAATTTA CGATTTGCAA ATCAAAAACA CATCTTATAA TCGAGATAAT 
GGACGCTTTG AGTGCCGCAT CAAGGCGAAG GGAACGGGCG CGGATGTCCA TCAGGAGTTC 
TACAATCTGA CCGTGCTGAC GGCTCCACAT CCGCCGATGG TCACTCCCGG CAATTTAGCA 
GTGGCCACCG AAGAGAAGCC ATTGGAGTTG ACCTGCAGCA GTATTGGTGG CTCACCAGAT 
CCAATGATAA CCTGGTACCG AGAGGGCAGC ACAGTGCCAC TGCAGTCGTA TGCTCTGAAG 
GGCGGATCGA AGAACCACTA TACAAACGCC ACACTCCAAA TTGTTCCCCG ACGGGCCGAC 
GATGGCGCCA AGTACAAATG CGTCGTCTGG AATCGTGCCA TGCCCGAGGG TCATATGCTG 
GAGACCAGCG TCACGCTGAA TGTCAACTAC TATCCCCGAG TGGAGGTCGG ACCCCAGAAT 
CCTTTAAAAG TAGAACGGGA TCATGTGGCG AAACTGGACT GCAGAGTGGA TGCCAAACCG 
ATGGTTAGCA ATGTGCGATG GTCCCGAAAT GGTCAATATG TGAGTGCCAC GCCCACCCAC 
ACCATATATA GAGTAAATCG CCATCATGCC GGGAAGTACA CCTGCAGTGC GGACAACGGC 
TTGGGCAAAA CTGGCGAAAA AGATATAGTC TTGGATGTTC TATATCCGCC GATTGTGTTT 
ATCGAATCGA AAACGCACGA AGCGGAGGAA GGTGAAACGG TTTTGATTCG TTGCAATGTC 
ACCGCAAATC CATCGCCCAT CAATGTCGAA TGGCTAAAAG AAGGAGCTCC GGACTTCCGA 
TACACTGGCG AGCTCTTAAC CCTCGGTTCA GTGAGGGCTG AGCACGCCGG AAACTATATC 
TGCAGATCGG TGAACATTAT GCAACCGTTC AGTTCGAAAC GTGTAGAGGG TGTGGGCAAT 
TCCACGGTGG CTCTATTGGT TCGACATCGG CCGGGCCAGG CTTATATAAC TCCGAATAAG 
CCAGTCGTTC ATGTGGGCAA TGGTGTGACT CTAACCTGTT CAGCGAATCC GCCCGGATGG 
CCTGTACCCC AATACCGATG GTTCCGAGAT ATGGATGGTG ACATTGGAAA TACACAGAAA 
ATTTTAGCGC AAGGTCCACA GTACTCTATA CCGAAAGCTC ACTTGGGAAG CGAGGGCAAG 
TACCACTGTC ATGCGGTCAA CGAGTTGGGA ATTGGTAAAA TAGCCACCAT AATCTTAGAG 
GTACATCAGC CACCCCAGTT TCTGGCCAAA CTACAGCAAC ATATGACACG TCGGGTGGGC 
GATGTAGACT ATGCGGTCAC CTGCAGTGCC AAGGGAAAGC CTACACCGCA AATTCGATGG 
ATCAAAGATG GTACCGAGAT TCTGCCCACT CGCAAGATGT TTGATATAAG AACTACACCC 
ACGGATGCCG GTGGCGGAGT GGTGGCGGTG CAAAGTATAC TGAGATTCAG GGGTAAGGCT 
AGACCGAATG GAAACCAACT ACTTCCGAAC GATAGAGGTC TGTATACTTG CCTTTACGAA 
AACGACGTAA ATTCGGCCAA TTCTTCAATG CATCTGAGGA TCGAGCACGA ACCGATAGTC 
ATTCATCAGT ACAACAAAGT GGCCTACGAT CTTCGAGAAT CCGCTGAGGT TGTCTGCAGG 
GTTCAGGCCT ATCCGAAACC GGAGTTTCAA TGGCAATACG GCAACAATCC TTCGCCATTG 
ACCATGTCTT CGGATGGTCA TTATGAAATT AGTACGAGAA TGGAGAATAA TGATGTGTAC 
ACCTCCATTT TGAGGATAGC ACACTTGCAG CACTCTGATT ACGGAGAATA TATTTGCAGA 
GCTGTAAATC CTTTGGACAG CATAAGAGCA CCAATAAGAC TGCAACCCAA AGGATCGCCA 
GAGAAACCCA CCAATTTAAA GATCCTCGAA GTTGGTCACA ACTATGCCGT CCTAAACTGG 
ACACCGGGAT TTAATGGCGG CTTTATGAGC ACCAAATATC TGGTGAGCTA CAGGAGAGTG 
GCAACACCCA GGGAACAAAC CCTATCCGAT TGTTCAGGAA ATGGCTATAT ACCCAGCTAT 
CAGATCAGCA GTTCGTCCAG TAATTCCAAC CACGAATGGA TCGAATTCAA TTGCTTTAAG 
GAGAACCCCT GTAAACTCGC ACCATTGGAT CAACATCAGA GTTATATGTT TAAGGTTTAT 
GCCCTGAATT CGAAGGGCAC CTCGGGATAT TCGAATGAGA TATTGGCCAC CACCAAAGTC 
AGTAAAATTC CACCGCCTTT GCATGTTAGC TACGATCCCA ATTCACATGT TCTGGGCATC 
AACGTGGCCG CCACCTGTCT GTCCCTCATT GCGGTGGTGG AATCACTGGT AACCAGGGAT 
GCCACGGTGC CCATGTGGGA GATTGTGGAA ACCCTGACTT TGCTCCCGTC GGGCAGCGAA 
ACGACCTTCA AGGAGGCCAT CATCAATCAT GTGTCGCGTC CAGCCCACTA CACCACCGCC 
ACCACGTCCG GAAGGAGCCT TGGAGTGGGT GGTGGCTCCC ACCTGGGCGA GGATCGCACC 
ATGGCGTTGG CCGAGACCGC GGGACCCGGT CCAGTGGTGC GGGTCAAACT CTGTCTGAGA 
TCTAACCACG AGCACTGTGG TGCATATGCC AATGCAGAAA TTGGCAAGTC CTACATGCCG 
CACAAATCCT CCATGACGAC ATCTGCATTA GTGGCCATCA TCATCGCTTC GCTTTCTTTC 
GTCGTCTTTC TTGGTCTTCT CTACGCTTTT TGCCACTGCC GTAGGAAACA TGCCGCCAAA 
AAGGAGAGTA GCTCAGTAGG AGGAGGAGTT GGAGGAGGCA ACGCTAACGC CACGGCTAAT 
CCAGGATCTA CCGGTGCCAA AGAGTACGAC CTCGACTTGG ACGCCAGCCG AAGACCGTCG 
CTAAGTCAGG ATCCACAGCA ATCGCAACAG CAGCCACCGC CACCACCACC CTACTATCCC 
ACGGGAACTC TGGACTCCAA GGACATTGGC AATGGGAACG GCGGCATGGA GCTCACCCTC 
ACCGCCCTAC ACGATCCTGA TGAGCAGCTG AATATGCAAC AGCAGCAGCA CCACAGCAAC 
CACGGCCAGT ATCAACAGCC CAAAGCCATA CTGGGTATCT ACGGCGGAGT GGCTGGCTCT 
GGGGGCAACA ATTCCGGCGG ACAGCATCCA CATTCGAACG GCTACGGCTA TCATGTGACA 
AGTGCCATCG GCGTGGACAG CGACAGCTAT CAAGTGCTGC CCTCCGTTGC CAACTCAGCA 
GCCGGATCCC ACGGACATGG CAGTGGACAC GGACACGGAC TAGGAGCCGG AGAGTCAGGA 
CCACTTGAGG CGACACCACC AACATGCAAC ATCAGCGGCG GCAGCAGCAG CAACAGCGGC 
ATTAACCCAA TGCAGCAGCA ACATTCAGCA CGCGCCAATC TAACGAACCA ACCCACCATC 
GCCACTGCCA GCTCCACCAA CAACTACAAC AACCACTTGA ACAACACCAA CATCGCCCAC 
ACCACCAACA ACACCAACAA CTGCACCACC CTGAAACGCG GCCACTTGGG AAATAGGGAG 
AGAGAGCGGG AAAGATGCCA GGTAACCGCA GCCACGGCGG CAACAACAAC GGCACTGGCA 
ACCACCATAA CAACAACATC TCGCAATGCC AAGGCAGCAA CAACAACAAC AACACTAGCC 
ATTACAGGGT CAAGCAGCAA TTCGAACGAG AATAACTACA GTAATGCCAG GGATCTGTCG 
CAGGAGGCAT TGTGGCGCCT CCAAATGGCC ACCGCCCAGT CGCAACAGAT TTACGTGGAG 
CGACCACCGT CGGCATTCAG TGGCCTGGTC GACTATAGTG GCTATTCGCC GCACATCCCC 
ACGGTGACCA GTTCGCTCAG CCAACAGAGT TTCAGTCCAA CGCAGCAATT AGCGCCCCAC 
GAAATGCTGC AGGCCGCCCA GCGTTACGGA ACTTTGAGGA AATCCGGAAA ACAACCGCCT 
CTTCCCCCGC AGCGAAAGGA TATGCAGCAG CAGGCGAAGC CACCTCAGCA AATGGCGGAT 
ATAATACAGG ATTTGGCAAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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fred cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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