fred cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following fred gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fred cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa13700 NM_164569.4
Latest version!
Drosophila melanogaster friend of echinoid (fred), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa16205 NM_001258937.2
Latest version!
Drosophila melanogaster friend of echinoid (fred), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa21586 NM_134968.4
Latest version!
Drosophila melanogaster friend of echinoid (fred), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa13700
    Clone ID Related Accession (Same CDS sequence) NM_164569.4
    Accession Version NM_164569.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product friend of echinoid, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On May 8, 2012 this sequence version replaced NM_164569.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCGACCA TAAAGCTGCT CATTATCGGT CAACTTTGGC TCTCGATTGG CCTGATCTCC 
    GGGGACGATT CGCTGGACAC GCGTGAGGGC GTCGATCTGG TGCTCAAGTG CCGCTTCACC
    GAGCATTACG ACTCGACCGA CTTCACCTTC TACTGGGCGC GATGGACCTG CTGCCCCACA
    TTGTTCGAGA ACGTGGCCAT TGGCGATGTG CAGCTCAACT CGAATTATCG CTTGGACTTC
    CGACCCAGTC GCGGAATTTA CGATTTGCAA ATCAAAAACA CATCTTATAA TCGAGATAAT
    GGACGCTTTG AGTGCCGCAT CAAGGCGAAG GGAACGGGCG CGGATGTCCA TCAGGAGTTC
    TACAATCTGA CCGTGCTGAC GGCTCCACAT CCGCCGATGG TCACTCCCGG CAATTTAGCA
    GTGGCCACCG AAGAGAAGCC ATTGGAGTTG ACCTGCAGCA GTATTGGTGG CTCACCAGAT
    CCAATGATAA CCTGGTACCG AGAGGGCAGC ACAGTGCCAC TGCAGTCGTA TGCTCTGAAG
    GGCGGATCGA AGAACCACTA TACAAACGCC ACACTCCAAA TTGTTCCCCG ACGGGCCGAC
    GATGGCGCCA AGTACAAATG CGTCGTCTGG AATCGTGCCA TGCCCGAGGG TCATATGCTG
    GAGACCAGCG TCACGCTGAA TGTCAACTAC TATCCCCGAG TGGAGGTCGG ACCCCAGAAT
    CCTTTAAAAG TAGAACGGGA TCATGTGGCG AAACTGGACT GCAGAGTGGA TGCCAAACCG
    ATGGTTAGCA ATGTGCGATG GTCCCGAAAT GGTCAATATG TGAGTGCCAC GCCCACCCAC
    ACCATATATA GAGTAAATCG CCATCATGCC GGGAAGTACA CCTGCAGTGC GGACAACGGC
    TTGGGCAAAA CTGGCGAAAA AGATATAGTC TTGGATGTTC TATATCCGCC GATTGTGTTT
    ATCGAATCGA AAACGCACGA AGCGGAGGAA GGTGAAACGG TTTTGATTCG TTGCAATGTC
    ACCGCAAATC CATCGCCCAT CAATGTCGAA TGGCTAAAAG AAGGAGCTCC GGACTTCCGA
    TACACTGGCG AGCTCTTAAC CCTCGGTTCA GTGAGGGCTG AGCACGCCGG AAACTATATC
    TGCAGATCGG TGAACATTAT GCAACCGTTC AGTTCGAAAC GTGTAGAGGG TGTGGGCAAT
    TCCACGGTGG CTCTATTGGT TCGACATCGG CCGGGCCAGG CTTATATAAC TCCGAATAAG
    CCAGTCGTTC ATGTGGGCAA TGGTGTGACT CTAACCTGTT CAGCGAATCC GCCCGGATGG
    CCTGTACCCC AATACCGATG GTTCCGAGAT ATGGATGGTG ACATTGGAAA TACACAGAAA
    ATTTTAGCGC AAGGTCCACA GTACTCTATA CCGAAAGCTC ACTTGGGAAG CGAGGGCAAG
    TACCACTGTC ATGCGGTCAA CGAGTTGGGA ATTGGTAAAA TAGCCACCAT AATCTTAGAG
    GTACATCAGC CACCCCAGTT TCTGGCCAAA CTACAGCAAC ATATGACACG TCGGGTGGGC
    GATGTAGACT ATGCGGTCAC CTGCAGTGCC AAGGGAAAGC CTACACCGCA AATTCGATGG
    ATCAAAGATG GTACCGAGAT TCTGCCCACT CGCAAGATGT TTGATATAAG AACTACACCC
    ACGGATGCCG GTGGCGGAGT GGTGGCGGTG CAAAGTATAC TGAGATTCAG GGGTAAGGCT
    AGACCGAATG GAAACCAACT ACTTCCGAAC GATAGAGGTC TGTATACTTG CCTTTACGAA
    AACGACGTAA ATTCGGCCAA TTCTTCAATG CATCTGAGGA TCGAGCACGA ACCGATAGTC
    ATTCATCAGT ACAACAAAGT GGCCTACGAT CTTCGAGAAT CCGCTGAGGT TGTCTGCAGG
    GTTCAGGCCT ATCCGAAACC GGAGTTTCAA TGGCAATACG GCAACAATCC TTCGCCATTG
    ACCATGTCTT CGGATGGTCA TTATGAAATT AGTACGAGAA TGGAGAATAA TGATGTGTAC
    ACCTCCATTT TGAGGATAGC ACACTTGCAG CACTCTGATT ACGGAGAATA TATTTGCAGA
    GCTGTAAATC CTTTGGACAG CATAAGAGCA CCAATAAGAC TGCAACCCAA AGGATCGCCA
    GAGAAACCCA CCAATTTAAA GATCCTCGAA GTTGGTCACA ACTATGCCGT CCTAAACTGG
    ACACCGGGAT TTAATGGCGG CTTTATGAGC ACCAAATATC TGGTGAGCTA CAGGAGAGTG
    GCAACACCCA GGGAACAAAC CCTATCCGAT TGTTCAGGAA ATGGCTATAT ACCCAGCTAT
    CAGATCAGCA GTTCGTCCAG TAATTCCAAC CACGAATGGA TCGAATTCAA TTGCTTTAAG
    GAGAACCCCT GTAAACTCGC ACCATTGGAT CAACATCAGA GTTATATGTT TAAGGTTTAT
    GCCCTGAATT CGAAGGGCAC CTCGGGATAT TCGAATGAGA TATTGGCCAC CACCAAAGTC
    AGTAAAATTC CACCGCCTTT GCATGTTAGC TACGATCCCA ATTCACATGT TCTGGGCATC
    AACGTGGCCG CCACCTGTCT GTCCCTCATT GCGGTGGTGG AATCACTGGT AACCAGGGAT
    GCCACGGTGC CCATGTGGGA GATTGTGGAA ACCCTGACTT TGCTCCCGTC GGGCAGCGAA
    ACGACCTTCA AGGAGGCCAT CATCAATCAT GTGTCGCGTC CAGCCCACTA CACCACCGCC
    ACCACGTCCG GAAGGAGCCT TGGAGTGGGT GGTGGCTCCC ACCTGGGCGA GGATCGCACC
    ATGGCGTTGG CCGAGACCGC GGGACCCGGT CCAGTGGTGC GGGTCAAACT CTGTCTGAGA
    TCTAACCACG AGCACTGTGG TGCATATGCC AATGCAGAAA TTGGCAAGTC CTACATGCCG
    CACAAATCCT CCATGACGAC ATCTGCATTA GTGGCCATCA TCATCGCTTC GCTTTCTTTC
    GTCGTCTTTC TTGGTCTTCT CTACGCTTTT TGCCACTGCC GTAGGAAACA TGCCGCCAAA
    AAGGAGAGTA GCTCAGTAGG AGGAGGAGTT GGAGGAGGCA ACGCTAACGC CACGGCTAAT
    CCAGGATCTA CCGGTGCCAA AGAGTACGAC CTCGACTTGG ACGCCAGCCG AAGACCGTCG
    CTAAGTCAGG ATCCACAGCA ATCGCAACAG CAGCCACCGC CACCACCACC CTACTATCCC
    ACGGGAACTC TGGACTCCAA GGACATTGGC AATGGGAACG GCGGCATGGA GCTCACCCTC
    ACCGCCCTAC ACGATCCTGA TGAGCAGCTG AATATGCAAC AGCAGCAGCA CCACAGCAAC
    CACGGCCAGT ATCAACAGCC CAAAGCCATA CTGGGTATCT ACGGCGGAGT GGCTGGCTCT
    GGGGGCAACA ATTCCGGCGG ACAGCATCCA CATTCGAACG GCTACGGCTA TCATGTGACA
    AGTGCCATCG GCGTGGACAG CGACAGCTAT CAAGTGCTGC CCTCCGTTGC CAACTCAGCA
    GCCGGATCCC ACGGACATGG CAGTGGACAC GGACACGGAC TAGGAGCCGG AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_722954.3
    CDS547..4143
    Translation

    Target ORF information:

    RefSeq Version NM_164569.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster friend of echinoid (fred), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_164569.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCGACCA TAAAGCTGCT CATTATCGGT CAACTTTGGC TCTCGATTGG CCTGATCTCC 
    GGGGACGATT CGCTGGACAC GCGTGAGGGC GTCGATCTGG TGCTCAAGTG CCGCTTCACC
    GAGCATTACG ACTCGACCGA CTTCACCTTC TACTGGGCGC GATGGACCTG CTGCCCCACA
    TTGTTCGAGA ACGTGGCCAT TGGCGATGTG CAGCTCAACT CGAATTATCG CTTGGACTTC
    CGACCCAGTC GCGGAATTTA CGATTTGCAA ATCAAAAACA CATCTTATAA TCGAGATAAT
    GGACGCTTTG AGTGCCGCAT CAAGGCGAAG GGAACGGGCG CGGATGTCCA TCAGGAGTTC
    TACAATCTGA CCGTGCTGAC GGCTCCACAT CCGCCGATGG TCACTCCCGG CAATTTAGCA
    GTGGCCACCG AAGAGAAGCC ATTGGAGTTG ACCTGCAGCA GTATTGGTGG CTCACCAGAT
    CCAATGATAA CCTGGTACCG AGAGGGCAGC ACAGTGCCAC TGCAGTCGTA TGCTCTGAAG
    GGCGGATCGA AGAACCACTA TACAAACGCC ACACTCCAAA TTGTTCCCCG ACGGGCCGAC
    GATGGCGCCA AGTACAAATG CGTCGTCTGG AATCGTGCCA TGCCCGAGGG TCATATGCTG
    GAGACCAGCG TCACGCTGAA TGTCAACTAC TATCCCCGAG TGGAGGTCGG ACCCCAGAAT
    CCTTTAAAAG TAGAACGGGA TCATGTGGCG AAACTGGACT GCAGAGTGGA TGCCAAACCG
    ATGGTTAGCA ATGTGCGATG GTCCCGAAAT GGTCAATATG TGAGTGCCAC GCCCACCCAC
    ACCATATATA GAGTAAATCG CCATCATGCC GGGAAGTACA CCTGCAGTGC GGACAACGGC
    TTGGGCAAAA CTGGCGAAAA AGATATAGTC TTGGATGTTC TATATCCGCC GATTGTGTTT
    ATCGAATCGA AAACGCACGA AGCGGAGGAA GGTGAAACGG TTTTGATTCG TTGCAATGTC
    ACCGCAAATC CATCGCCCAT CAATGTCGAA TGGCTAAAAG AAGGAGCTCC GGACTTCCGA
    TACACTGGCG AGCTCTTAAC CCTCGGTTCA GTGAGGGCTG AGCACGCCGG AAACTATATC
    TGCAGATCGG TGAACATTAT GCAACCGTTC AGTTCGAAAC GTGTAGAGGG TGTGGGCAAT
    TCCACGGTGG CTCTATTGGT TCGACATCGG CCGGGCCAGG CTTATATAAC TCCGAATAAG
    CCAGTCGTTC ATGTGGGCAA TGGTGTGACT CTAACCTGTT CAGCGAATCC GCCCGGATGG
    CCTGTACCCC AATACCGATG GTTCCGAGAT ATGGATGGTG ACATTGGAAA TACACAGAAA
    ATTTTAGCGC AAGGTCCACA GTACTCTATA CCGAAAGCTC ACTTGGGAAG CGAGGGCAAG
    TACCACTGTC ATGCGGTCAA CGAGTTGGGA ATTGGTAAAA TAGCCACCAT AATCTTAGAG
    GTACATCAGC CACCCCAGTT TCTGGCCAAA CTACAGCAAC ATATGACACG TCGGGTGGGC
    GATGTAGACT ATGCGGTCAC CTGCAGTGCC AAGGGAAAGC CTACACCGCA AATTCGATGG
    ATCAAAGATG GTACCGAGAT TCTGCCCACT CGCAAGATGT TTGATATAAG AACTACACCC
    ACGGATGCCG GTGGCGGAGT GGTGGCGGTG CAAAGTATAC TGAGATTCAG GGGTAAGGCT
    AGACCGAATG GAAACCAACT ACTTCCGAAC GATAGAGGTC TGTATACTTG CCTTTACGAA
    AACGACGTAA ATTCGGCCAA TTCTTCAATG CATCTGAGGA TCGAGCACGA ACCGATAGTC
    ATTCATCAGT ACAACAAAGT GGCCTACGAT CTTCGAGAAT CCGCTGAGGT TGTCTGCAGG
    GTTCAGGCCT ATCCGAAACC GGAGTTTCAA TGGCAATACG GCAACAATCC TTCGCCATTG
    ACCATGTCTT CGGATGGTCA TTATGAAATT AGTACGAGAA TGGAGAATAA TGATGTGTAC
    ACCTCCATTT TGAGGATAGC ACACTTGCAG CACTCTGATT ACGGAGAATA TATTTGCAGA
    GCTGTAAATC CTTTGGACAG CATAAGAGCA CCAATAAGAC TGCAACCCAA AGGATCGCCA
    GAGAAACCCA CCAATTTAAA GATCCTCGAA GTTGGTCACA ACTATGCCGT CCTAAACTGG
    ACACCGGGAT TTAATGGCGG CTTTATGAGC ACCAAATATC TGGTGAGCTA CAGGAGAGTG
    GCAACACCCA GGGAACAAAC CCTATCCGAT TGTTCAGGAA ATGGCTATAT ACCCAGCTAT
    CAGATCAGCA GTTCGTCCAG TAATTCCAAC CACGAATGGA TCGAATTCAA TTGCTTTAAG
    GAGAACCCCT GTAAACTCGC ACCATTGGAT CAACATCAGA GTTATATGTT TAAGGTTTAT
    GCCCTGAATT CGAAGGGCAC CTCGGGATAT TCGAATGAGA TATTGGCCAC CACCAAAGTC
    AGTAAAATTC CACCGCCTTT GCATGTTAGC TACGATCCCA ATTCACATGT TCTGGGCATC
    AACGTGGCCG CCACCTGTCT GTCCCTCATT GCGGTGGTGG AATCACTGGT AACCAGGGAT
    GCCACGGTGC CCATGTGGGA GATTGTGGAA ACCCTGACTT TGCTCCCGTC GGGCAGCGAA
    ACGACCTTCA AGGAGGCCAT CATCAATCAT GTGTCGCGTC CAGCCCACTA CACCACCGCC
    ACCACGTCCG GAAGGAGCCT TGGAGTGGGT GGTGGCTCCC ACCTGGGCGA GGATCGCACC
    ATGGCGTTGG CCGAGACCGC GGGACCCGGT CCAGTGGTGC GGGTCAAACT CTGTCTGAGA
    TCTAACCACG AGCACTGTGG TGCATATGCC AATGCAGAAA TTGGCAAGTC CTACATGCCG
    CACAAATCCT CCATGACGAC ATCTGCATTA GTGGCCATCA TCATCGCTTC GCTTTCTTTC
    GTCGTCTTTC TTGGTCTTCT CTACGCTTTT TGCCACTGCC GTAGGAAACA TGCCGCCAAA
    AAGGAGAGTA GCTCAGTAGG AGGAGGAGTT GGAGGAGGCA ACGCTAACGC CACGGCTAAT
    CCAGGATCTA CCGGTGCCAA AGAGTACGAC CTCGACTTGG ACGCCAGCCG AAGACCGTCG
    CTAAGTCAGG ATCCACAGCA ATCGCAACAG CAGCCACCGC CACCACCACC CTACTATCCC
    ACGGGAACTC TGGACTCCAA GGACATTGGC AATGGGAACG GCGGCATGGA GCTCACCCTC
    ACCGCCCTAC ACGATCCTGA TGAGCAGCTG AATATGCAAC AGCAGCAGCA CCACAGCAAC
    CACGGCCAGT ATCAACAGCC CAAAGCCATA CTGGGTATCT ACGGCGGAGT GGCTGGCTCT
    GGGGGCAACA ATTCCGGCGG ACAGCATCCA CATTCGAACG GCTACGGCTA TCATGTGACA
    AGTGCCATCG GCGTGGACAG CGACAGCTAT CAAGTGCTGC CCTCCGTTGC CAACTCAGCA
    GCCGGATCCC ACGGACATGG CAGTGGACAC GGACACGGAC TAGGAGCCGG AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16205
    Clone ID Related Accession (Same CDS sequence) NM_001258937.2
    Accession Version NM_001258937.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product friend of echinoid, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001258937.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCGACCA TAAAGCTGCT CATTATCGGT CAACTTTGGC TCTCGATTGG CCTGATCTCC 
    GGGGACGATT CGCTGGACAC GCGTGAGGGC GTCGATCTGG TGCTCAAGTG CCGCTTCACC
    GAGCATTACG ACTCGACCGA CTTCACCTTC TACTGGGCGC GATGGACCTG CTGCCCCACA
    TTGTTCGAGA ACGTGGCCAT TGGCGATGTG CAGCTCAACT CGAATTATCG CTTGGACTTC
    CGACCCAGTC GCGGAATTTA CGATTTGCAA ATCAAAAACA CATCTTATAA TCGAGATAAT
    GGACGCTTTG AGTGCCGCAT CAAGGCGAAG GGAACGGGCG CGGATGTCCA TCAGGAGTTC
    TACAATCTGA CCGTGCTGAC GGCTCCACAT CCGCCGATGG TCACTCCCGG CAATTTAGCA
    GTGGCCACCG AAGAGAAGCC ATTGGAGTTG ACCTGCAGCA GTATTGGTGG CTCACCAGAT
    CCAATGATAA CCTGGTACCG AGAGGGCAGC ACAGTGCCAC TGCAGTCGTA TGCTCTGAAG
    GGCGGATCGA AGAACCACTA TACAAACGCC ACACTCCAAA TTGTTCCCCG ACGGGCCGAC
    GATGGCGCCA AGTACAAATG CGTCGTCTGG AATCGTGCCA TGCCCGAGGG TCATATGCTG
    GAGACCAGCG TCACGCTGAA TGTCAACTAC TATCCCCGAG TGGAGGTCGG ACCCCAGAAT
    CCTTTAAAAG TAGAACGGGA TCATGTGGCG AAACTGGACT GCAGAGTGGA TGCCAAACCG
    ATGGTTAGCA ATGTGCGATG GTCCCGAAAT GGTCAATATG TGAGTGCCAC GCCCACCCAC
    ACCATATATA GAGTAAATCG CCATCATGCC GGGAAGTACA CCTGCAGTGC GGACAACGGC
    TTGGGCAAAA CTGGCGAAAA AGATATAGTC TTGGATGTTC TATATCCGCC GATTGTGTTT
    ATCGAATCGA AAACGCACGA AGCGGAGGAA GGTGAAACGG TTTTGATTCG TTGCAATGTC
    ACCGCAAATC CATCGCCCAT CAATGTCGAA TGGCTAAAAG AAGGAGCTCC GGACTTCCGA
    TACACTGGCG AGCTCTTAAC CCTCGGTTCA GTGAGGGCTG AGCACGCCGG AAACTATATC
    TGCAGATCGG TGAACATTAT GCAACCGTTC AGTTCGAAAC GTGTAGAGGG TGTGGGCAAT
    TCCACGGTGG CTCTATTGGT TCGACATCGG CCGGGCCAGG CTTATATAAC TCCGAATAAG
    CCAGTCGTTC ATGTGGGCAA TGGTGTGACT CTAACCTGTT CAGCGAATCC GCCCGGATGG
    CCTGTACCCC AATACCGATG GTTCCGAGAT ATGGATGGTG ACATTGGAAA TACACAGAAA
    ATTTTAGCGC AAGGTCCACA GTACTCTATA CCGAAAGCTC ACTTGGGAAG CGAGGGCAAG
    TACCACTGTC ATGCGGTCAA CGAGTTGGGA ATTGGTAAAA TAGCCACCAT AATCTTAGAG
    GTACATCAGC CACCCCAGTT TCTGGCCAAA CTACAGCAAC ATATGACACG TCGGGTGGGC
    GATGTAGACT ATGCGGTCAC CTGCAGTGCC AAGGGAAAGC CTACACCGCA AATTCGATGG
    ATCAAAGATG GTACCGAGAT TCTGCCCACT CGCAAGATGT TTGATATAAG AACTACACCC
    ACGGATGCCG GTGGCGGAGT GGTGGCGGTG CAAAGTATAC TGAGATTCAG GGGTAAGGCT
    AGACCGAATG GAAACCAACT ACTTCCGAAC GATAGAGGTC TGTATACTTG CCTTTACGAA
    AACGACGTAA ATTCGGCCAA TTCTTCAATG CATCTGAGGA TCGAGCACGA ACCGATAGTC
    ATTCATCAGT ACAACAAAGT GGCCTACGAT CTTCGAGAAT CCGCTGAGGT TGTCTGCAGG
    GTTCAGGCCT ATCCGAAACC GGAGTTTCAA TGGCAATACG GCAACAATCC TTCGCCATTG
    ACCATGTCTT CGGATGGTCA TTATGAAATT AGTACGAGAA TGGAGAATAA TGATGTGTAC
    ACCTCCATTT TGAGGATAGC ACACTTGCAG CACTCTGATT ACGGAGAATA TATTTGCAGA
    GCTGTAAATC CTTTGGACAG CATAAGAGCA CCAATAAGAC TGCAACCCAA AGGATCGCCA
    GAGAAACCCA CCAATTTAAA GATCCTCGAA GTTGGTCACA ACTATGCCGT CCTAAACTGG
    ACACCGGGAT TTAATGGCGG CTTTATGAGC ACCAAATATC TGGTGAGCTA CAGGAGAGTG
    GCAACACCCA GGGAACAAAC CCTATCCGAT TGTTCAGGAA ATGGCTATAT ACCCAGCTAT
    CAGATCAGCA GTTCGTCCAG TAATTCCAAC CACGAATGGA TCGAATTCAA TTGCTTTAAG
    GAGAACCCCT GTAAACTCGC ACCATTGGAT CAACATCAGA GTTATATGTT TAAGGTTTAT
    GCCCTGAATT CGAAGGGCAC CTCGGGATAT TCGAATGAGA TATTGGCCAC CACCAAAGTC
    AGTAAAATTC CACCGCCTTT GCATGTTAGC TACGATCCCA ATTCACATGT TCTGGGCATC
    AACGTGGCCG CCACCTGTCT GTCCCTCATT GCGGTGGTGG AATCACTGGT AACCAGGGAT
    GCCACGGTGC CCATGTGGGA GATTGTGGAA ACCCTGACTT TGCTCCCGTC GGGCAGCGAA
    ACGACCTTCA AGGAGGCCAT CATCAATCAT GTGTCGCGTC CAGCCCACTA CACCACCGCC
    ACCACGTCCG GAAGGAGCCT TGGAGTGGGT GGTGGCTCCC ACCTGGGCGA GGATCGCACC
    ATGGCGTTGG CCGAGACCGC GGGACCCGGT CCAGTGGTGC GGGTCAAACT CTGTCTGAGA
    TCTAACCACG AGCACTGTGG TGCATATGCC AATGCAGAAA TTGGCAAGTC CTACATGCCG
    CACAAATCCT CCATGACGAC ATCTGCATTA GTGGCCATCA TCATCGCTTC GCTTTCTTTC
    GTCGTCTTTC TTGGTCTTCT CTACGCTTTT TGCCACTGCC GTAGGAAACA TGCCGCCAAA
    AAGGAGAGTA GCTCAGTAGG AGGAGGAGTT GGAGGAGGCA ACGCTAACGC CACGGCTAAT
    CCAGGATCTA CCGGTGCCAA AGAGTACGAC CTCGACTTGG ACGCCAGCCG AAGACCGTCG
    CTAAGTCAGG ATCCACAGCA ATCGCAACAG CAGCCACCGC CACCACCACC CTACTATCCC
    ACGGGAACTC TGGACTCCAA GGACATTGGC AATGGGAACG GCGGCATGGA GCTCACCCTC
    ACCGCCCTAC ACGATCCTGA TGAGCAGCTG AATATGCAAC AGCAGCAGCA CCACAGCAAC
    CACGGCCAGT ATCAACAGCC CAAAGCCATA CTGGGTATCT ACGGCGGAGT GGCTGGCTCT
    GGGGGCAACA ATTCCGGCGG ACAGCATCCA CATTCGAACG GCTACGGCTA TCATGTGACA
    AGTGCCATCG GCGTGGACAG CGACAGCTAT CAAGTGCTGC CCTCCGTTGC CAACTCAGCA
    GCCGGATCCC ACGGACATGG CAGTGGACAC GGACACGGAC TAGGAGCCGG AGAGTGGTCA
    AGCAGCAATT CGAACGAGAA TAACTACAGT AATGCCAGGG ATCTGTCGCA GGAGGCATTG
    TGGCGCCTCC AAATGGCCAC CGCCCAGTCG CAACAGATTT ACGTGGAGCG ACCACCGTCG
    GCATTCAGTG GCCTGGTCGA CTATAGTGGC TATTCGCCGC ACATCCCCAC GGTGACCAGT
    TCGCTCAGCC AACAGAGTTT CAGTCCAACG CAGCAATTAG CGCCCCACGA AATGCTGCAG
    GCCGCCCAGC GTTACGGAAC TTTGAGGAAA TCCGGAAAAC AACCGCCTCT TCCCCCGCAG
    CGAAAGGATA TGCAGCAGCA GGCGAAGCCA CCTCAGCAAA TGGCGGATAT AATACAGGAT
    TTGGCAAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001245866.1
    CDS547..4518
    Translation

    Target ORF information:

    RefSeq Version NM_001258937.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster friend of echinoid (fred), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001258937.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTCGACCA TAAAGCTGCT CATTATCGGT CAACTTTGGC TCTCGATTGG CCTGATCTCC 
    GGGGACGATT CGCTGGACAC GCGTGAGGGC GTCGATCTGG TGCTCAAGTG CCGCTTCACC
    GAGCATTACG ACTCGACCGA CTTCACCTTC TACTGGGCGC GATGGACCTG CTGCCCCACA
    TTGTTCGAGA ACGTGGCCAT TGGCGATGTG CAGCTCAACT CGAATTATCG CTTGGACTTC
    CGACCCAGTC GCGGAATTTA CGATTTGCAA ATCAAAAACA CATCTTATAA TCGAGATAAT
    GGACGCTTTG AGTGCCGCAT CAAGGCGAAG GGAACGGGCG CGGATGTCCA TCAGGAGTTC
    TACAATCTGA CCGTGCTGAC GGCTCCACAT CCGCCGATGG TCACTCCCGG CAATTTAGCA
    GTGGCCACCG AAGAGAAGCC ATTGGAGTTG ACCTGCAGCA GTATTGGTGG CTCACCAGAT
    CCAATGATAA CCTGGTACCG AGAGGGCAGC ACAGTGCCAC TGCAGTCGTA TGCTCTGAAG
    GGCGGATCGA AGAACCACTA TACAAACGCC ACACTCCAAA TTGTTCCCCG ACGGGCCGAC
    GATGGCGCCA AGTACAAATG CGTCGTCTGG AATCGTGCCA TGCCCGAGGG TCATATGCTG
    GAGACCAGCG TCACGCTGAA TGTCAACTAC TATCCCCGAG TGGAGGTCGG ACCCCAGAAT
    CCTTTAAAAG TAGAACGGGA TCATGTGGCG AAACTGGACT GCAGAGTGGA TGCCAAACCG
    ATGGTTAGCA ATGTGCGATG GTCCCGAAAT GGTCAATATG TGAGTGCCAC GCCCACCCAC
    ACCATATATA GAGTAAATCG CCATCATGCC GGGAAGTACA CCTGCAGTGC GGACAACGGC
    TTGGGCAAAA CTGGCGAAAA AGATATAGTC TTGGATGTTC TATATCCGCC GATTGTGTTT
    ATCGAATCGA AAACGCACGA AGCGGAGGAA GGTGAAACGG TTTTGATTCG TTGCAATGTC
    ACCGCAAATC CATCGCCCAT CAATGTCGAA TGGCTAAAAG AAGGAGCTCC GGACTTCCGA
    TACACTGGCG AGCTCTTAAC CCTCGGTTCA GTGAGGGCTG AGCACGCCGG AAACTATATC
    TGCAGATCGG TGAACATTAT GCAACCGTTC AGTTCGAAAC GTGTAGAGGG TGTGGGCAAT
    TCCACGGTGG CTCTATTGGT TCGACATCGG CCGGGCCAGG CTTATATAAC TCCGAATAAG
    CCAGTCGTTC ATGTGGGCAA TGGTGTGACT CTAACCTGTT CAGCGAATCC GCCCGGATGG
    CCTGTACCCC AATACCGATG GTTCCGAGAT ATGGATGGTG ACATTGGAAA TACACAGAAA
    ATTTTAGCGC AAGGTCCACA GTACTCTATA CCGAAAGCTC ACTTGGGAAG CGAGGGCAAG
    TACCACTGTC ATGCGGTCAA CGAGTTGGGA ATTGGTAAAA TAGCCACCAT AATCTTAGAG
    GTACATCAGC CACCCCAGTT TCTGGCCAAA CTACAGCAAC ATATGACACG TCGGGTGGGC
    GATGTAGACT ATGCGGTCAC CTGCAGTGCC AAGGGAAAGC CTACACCGCA AATTCGATGG
    ATCAAAGATG GTACCGAGAT TCTGCCCACT CGCAAGATGT TTGATATAAG AACTACACCC
    ACGGATGCCG GTGGCGGAGT GGTGGCGGTG CAAAGTATAC TGAGATTCAG GGGTAAGGCT
    AGACCGAATG GAAACCAACT ACTTCCGAAC GATAGAGGTC TGTATACTTG CCTTTACGAA
    AACGACGTAA ATTCGGCCAA TTCTTCAATG CATCTGAGGA TCGAGCACGA ACCGATAGTC
    ATTCATCAGT ACAACAAAGT GGCCTACGAT CTTCGAGAAT CCGCTGAGGT TGTCTGCAGG
    GTTCAGGCCT ATCCGAAACC GGAGTTTCAA TGGCAATACG GCAACAATCC TTCGCCATTG
    ACCATGTCTT CGGATGGTCA TTATGAAATT AGTACGAGAA TGGAGAATAA TGATGTGTAC
    ACCTCCATTT TGAGGATAGC ACACTTGCAG CACTCTGATT ACGGAGAATA TATTTGCAGA
    GCTGTAAATC CTTTGGACAG CATAAGAGCA CCAATAAGAC TGCAACCCAA AGGATCGCCA
    GAGAAACCCA CCAATTTAAA GATCCTCGAA GTTGGTCACA ACTATGCCGT CCTAAACTGG
    ACACCGGGAT TTAATGGCGG CTTTATGAGC ACCAAATATC TGGTGAGCTA CAGGAGAGTG
    GCAACACCCA GGGAACAAAC CCTATCCGAT TGTTCAGGAA ATGGCTATAT ACCCAGCTAT
    CAGATCAGCA GTTCGTCCAG TAATTCCAAC CACGAATGGA TCGAATTCAA TTGCTTTAAG
    GAGAACCCCT GTAAACTCGC ACCATTGGAT CAACATCAGA GTTATATGTT TAAGGTTTAT
    GCCCTGAATT CGAAGGGCAC CTCGGGATAT TCGAATGAGA TATTGGCCAC CACCAAAGTC
    AGTAAAATTC CACCGCCTTT GCATGTTAGC TACGATCCCA ATTCACATGT TCTGGGCATC
    AACGTGGCCG CCACCTGTCT GTCCCTCATT GCGGTGGTGG AATCACTGGT AACCAGGGAT
    GCCACGGTGC CCATGTGGGA GATTGTGGAA ACCCTGACTT TGCTCCCGTC GGGCAGCGAA
    ACGACCTTCA AGGAGGCCAT CATCAATCAT GTGTCGCGTC CAGCCCACTA CACCACCGCC
    ACCACGTCCG GAAGGAGCCT TGGAGTGGGT GGTGGCTCCC ACCTGGGCGA GGATCGCACC
    ATGGCGTTGG CCGAGACCGC GGGACCCGGT CCAGTGGTGC GGGTCAAACT CTGTCTGAGA
    TCTAACCACG AGCACTGTGG TGCATATGCC AATGCAGAAA TTGGCAAGTC CTACATGCCG
    CACAAATCCT CCATGACGAC ATCTGCATTA GTGGCCATCA TCATCGCTTC GCTTTCTTTC
    GTCGTCTTTC TTGGTCTTCT CTACGCTTTT TGCCACTGCC GTAGGAAACA TGCCGCCAAA
    AAGGAGAGTA GCTCAGTAGG AGGAGGAGTT GGAGGAGGCA ACGCTAACGC CACGGCTAAT
    CCAGGATCTA CCGGTGCCAA AGAGTACGAC CTCGACTTGG ACGCCAGCCG AAGACCGTCG
    CTAAGTCAGG ATCCACAGCA ATCGCAACAG CAGCCACCGC CACCACCACC CTACTATCCC
    ACGGGAACTC TGGACTCCAA GGACATTGGC AATGGGAACG GCGGCATGGA GCTCACCCTC
    ACCGCCCTAC ACGATCCTGA TGAGCAGCTG AATATGCAAC AGCAGCAGCA CCACAGCAAC
    CACGGCCAGT ATCAACAGCC CAAAGCCATA CTGGGTATCT ACGGCGGAGT GGCTGGCTCT
    GGGGGCAACA ATTCCGGCGG ACAGCATCCA CATTCGAACG GCTACGGCTA TCATGTGACA
    AGTGCCATCG GCGTGGACAG CGACAGCTAT CAAGTGCTGC CCTCCGTTGC CAACTCAGCA
    GCCGGATCCC ACGGACATGG CAGTGGACAC GGACACGGAC TAGGAGCCGG AGAGTGGTCA
    AGCAGCAATT CGAACGAGAA TAACTACAGT AATGCCAGGG ATCTGTCGCA GGAGGCATTG
    TGGCGCCTCC AAATGGCCAC CGCCCAGTCG CAACAGATTT ACGTGGAGCG ACCACCGTCG
    GCATTCAGTG GCCTGGTCGA CTATAGTGGC TATTCGCCGC ACATCCCCAC GGTGACCAGT
    TCGCTCAGCC AACAGAGTTT CAGTCCAACG CAGCAATTAG CGCCCCACGA AATGCTGCAG
    GCCGCCCAGC GTTACGGAAC TTTGAGGAAA TCCGGAAAAC AACCGCCTCT TCCCCCGCAG
    CGAAAGGATA TGCAGCAGCA GGCGAAGCCA CCTCAGCAAA TGGCGGATAT AATACAGGAT
    TTGGCAAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa21586
    Clone ID Related Accession (Same CDS sequence) NM_134968.4
    Accession Version NM_134968.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4344bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product friend of echinoid, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_134968.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCGACCA TAAAGCTGCT CATTATCGGT CAACTTTGGC TCTCGATTGG CCTGATCTCC 
    GGGGACGATT CGCTGGACAC GCGTGAGGGC GTCGATCTGG TGCTCAAGTG CCGCTTCACC
    GAGCATTACG ACTCGACCGA CTTCACCTTC TACTGGGCGC GATGGACCTG CTGCCCCACA
    TTGTTCGAGA ACGTGGCCAT TGGCGATGTG CAGCTCAACT CGAATTATCG CTTGGACTTC
    CGACCCAGTC GCGGAATTTA CGATTTGCAA ATCAAAAACA CATCTTATAA TCGAGATAAT
    GGACGCTTTG AGTGCCGCAT CAAGGCGAAG GGAACGGGCG CGGATGTCCA TCAGGAGTTC
    TACAATCTGA CCGTGCTGAC GGCTCCACAT CCGCCGATGG TCACTCCCGG CAATTTAGCA
    GTGGCCACCG AAGAGAAGCC ATTGGAGTTG ACCTGCAGCA GTATTGGTGG CTCACCAGAT
    CCAATGATAA CCTGGTACCG AGAGGGCAGC ACAGTGCCAC TGCAGTCGTA TGCTCTGAAG
    GGCGGATCGA AGAACCACTA TACAAACGCC ACACTCCAAA TTGTTCCCCG ACGGGCCGAC
    GATGGCGCCA AGTACAAATG CGTCGTCTGG AATCGTGCCA TGCCCGAGGG TCATATGCTG
    GAGACCAGCG TCACGCTGAA TGTCAACTAC TATCCCCGAG TGGAGGTCGG ACCCCAGAAT
    CCTTTAAAAG TAGAACGGGA TCATGTGGCG AAACTGGACT GCAGAGTGGA TGCCAAACCG
    ATGGTTAGCA ATGTGCGATG GTCCCGAAAT GGTCAATATG TGAGTGCCAC GCCCACCCAC
    ACCATATATA GAGTAAATCG CCATCATGCC GGGAAGTACA CCTGCAGTGC GGACAACGGC
    TTGGGCAAAA CTGGCGAAAA AGATATAGTC TTGGATGTTC TATATCCGCC GATTGTGTTT
    ATCGAATCGA AAACGCACGA AGCGGAGGAA GGTGAAACGG TTTTGATTCG TTGCAATGTC
    ACCGCAAATC CATCGCCCAT CAATGTCGAA TGGCTAAAAG AAGGAGCTCC GGACTTCCGA
    TACACTGGCG AGCTCTTAAC CCTCGGTTCA GTGAGGGCTG AGCACGCCGG AAACTATATC
    TGCAGATCGG TGAACATTAT GCAACCGTTC AGTTCGAAAC GTGTAGAGGG TGTGGGCAAT
    TCCACGGTGG CTCTATTGGT TCGACATCGG CCGGGCCAGG CTTATATAAC TCCGAATAAG
    CCAGTCGTTC ATGTGGGCAA TGGTGTGACT CTAACCTGTT CAGCGAATCC GCCCGGATGG
    CCTGTACCCC AATACCGATG GTTCCGAGAT ATGGATGGTG ACATTGGAAA TACACAGAAA
    ATTTTAGCGC AAGGTCCACA GTACTCTATA CCGAAAGCTC ACTTGGGAAG CGAGGGCAAG
    TACCACTGTC ATGCGGTCAA CGAGTTGGGA ATTGGTAAAA TAGCCACCAT AATCTTAGAG
    GTACATCAGC CACCCCAGTT TCTGGCCAAA CTACAGCAAC ATATGACACG TCGGGTGGGC
    GATGTAGACT ATGCGGTCAC CTGCAGTGCC AAGGGAAAGC CTACACCGCA AATTCGATGG
    ATCAAAGATG GTACCGAGAT TCTGCCCACT CGCAAGATGT TTGATATAAG AACTACACCC
    ACGGATGCCG GTGGCGGAGT GGTGGCGGTG CAAAGTATAC TGAGATTCAG GGGTAAGGCT
    AGACCGAATG GAAACCAACT ACTTCCGAAC GATAGAGGTC TGTATACTTG CCTTTACGAA
    AACGACGTAA ATTCGGCCAA TTCTTCAATG CATCTGAGGA TCGAGCACGA ACCGATAGTC
    ATTCATCAGT ACAACAAAGT GGCCTACGAT CTTCGAGAAT CCGCTGAGGT TGTCTGCAGG
    GTTCAGGCCT ATCCGAAACC GGAGTTTCAA TGGCAATACG GCAACAATCC TTCGCCATTG
    ACCATGTCTT CGGATGGTCA TTATGAAATT AGTACGAGAA TGGAGAATAA TGATGTGTAC
    ACCTCCATTT TGAGGATAGC ACACTTGCAG CACTCTGATT ACGGAGAATA TATTTGCAGA
    GCTGTAAATC CTTTGGACAG CATAAGAGCA CCAATAAGAC TGCAACCCAA AGGATCGCCA
    GAGAAACCCA CCAATTTAAA GATCCTCGAA GTTGGTCACA ACTATGCCGT CCTAAACTGG
    ACACCGGGAT TTAATGGCGG CTTTATGAGC ACCAAATATC TGGTGAGCTA CAGGAGAGTG
    GCAACACCCA GGGAACAAAC CCTATCCGAT TGTTCAGGAA ATGGCTATAT ACCCAGCTAT
    CAGATCAGCA GTTCGTCCAG TAATTCCAAC CACGAATGGA TCGAATTCAA TTGCTTTAAG
    GAGAACCCCT GTAAACTCGC ACCATTGGAT CAACATCAGA GTTATATGTT TAAGGTTTAT
    GCCCTGAATT CGAAGGGCAC CTCGGGATAT TCGAATGAGA TATTGGCCAC CACCAAAGTC
    AGTAAAATTC CACCGCCTTT GCATGTTAGC TACGATCCCA ATTCACATGT TCTGGGCATC
    AACGTGGCCG CCACCTGTCT GTCCCTCATT GCGGTGGTGG AATCACTGGT AACCAGGGAT
    GCCACGGTGC CCATGTGGGA GATTGTGGAA ACCCTGACTT TGCTCCCGTC GGGCAGCGAA
    ACGACCTTCA AGGAGGCCAT CATCAATCAT GTGTCGCGTC CAGCCCACTA CACCACCGCC
    ACCACGTCCG GAAGGAGCCT TGGAGTGGGT GGTGGCTCCC ACCTGGGCGA GGATCGCACC
    ATGGCGTTGG CCGAGACCGC GGGACCCGGT CCAGTGGTGC GGGTCAAACT CTGTCTGAGA
    TCTAACCACG AGCACTGTGG TGCATATGCC AATGCAGAAA TTGGCAAGTC CTACATGCCG
    CACAAATCCT CCATGACGAC ATCTGCATTA GTGGCCATCA TCATCGCTTC GCTTTCTTTC
    GTCGTCTTTC TTGGTCTTCT CTACGCTTTT TGCCACTGCC GTAGGAAACA TGCCGCCAAA
    AAGGAGAGTA GCTCAGTAGG AGGAGGAGTT GGAGGAGGCA ACGCTAACGC CACGGCTAAT
    CCAGGATCTA CCGGTGCCAA AGAGTACGAC CTCGACTTGG ACGCCAGCCG AAGACCGTCG
    CTAAGTCAGG ATCCACAGCA ATCGCAACAG CAGCCACCGC CACCACCACC CTACTATCCC
    ACGGGAACTC TGGACTCCAA GGACATTGGC AATGGGAACG GCGGCATGGA GCTCACCCTC
    ACCGCCCTAC ACGATCCTGA TGAGCAGCTG AATATGCAAC AGCAGCAGCA CCACAGCAAC
    CACGGCCAGT ATCAACAGCC CAAAGCCATA CTGGGTATCT ACGGCGGAGT GGCTGGCTCT
    GGGGGCAACA ATTCCGGCGG ACAGCATCCA CATTCGAACG GCTACGGCTA TCATGTGACA
    AGTGCCATCG GCGTGGACAG CGACAGCTAT CAAGTGCTGC CCTCCGTTGC CAACTCAGCA
    GCCGGATCCC ACGGACATGG CAGTGGACAC GGACACGGAC TAGGAGCCGG AGAGTCAGGA
    CCACTTGAGG CGACACCACC AACATGCAAC ATCAGCGGCG GCAGCAGCAG CAACAGCGGC
    ATTAACCCAA TGCAGCAGCA ACATTCAGCA CGCGCCAATC TAACGAACCA ACCCACCATC
    GCCACTGCCA GCTCCACCAA CAACTACAAC AACCACTTGA ACAACACCAA CATCGCCCAC
    ACCACCAACA ACACCAACAA CTGCACCACC CTGAAACGCG GCCACTTGGG AAATAGGGAG
    AGAGAGCGGG AAAGATGCCA GGTAACCGCA GCCACGGCGG CAACAACAAC GGCACTGGCA
    ACCACCATAA CAACAACATC TCGCAATGCC AAGGCAGCAA CAACAACAAC AACACTAGCC
    ATTACAGGGT CAAGCAGCAA TTCGAACGAG AATAACTACA GTAATGCCAG GGATCTGTCG
    CAGGAGGCAT TGTGGCGCCT CCAAATGGCC ACCGCCCAGT CGCAACAGAT TTACGTGGAG
    CGACCACCGT CGGCATTCAG TGGCCTGGTC GACTATAGTG GCTATTCGCC GCACATCCCC
    ACGGTGACCA GTTCGCTCAG CCAACAGAGT TTCAGTCCAA CGCAGCAATT AGCGCCCCAC
    GAAATGCTGC AGGCCGCCCA GCGTTACGGA ACTTTGAGGA AATCCGGAAA ACAACCGCCT
    CTTCCCCCGC AGCGAAAGGA TATGCAGCAG CAGGCGAAGC CACCTCAGCA AATGGCGGAT
    ATAATACAGG ATTTGGCAAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_608812.3
    CDS547..4890
    Translation

    Target ORF information:

    RefSeq Version NM_134968.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster friend of echinoid (fred), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_134968.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCGACCA TAAAGCTGCT CATTATCGGT CAACTTTGGC TCTCGATTGG CCTGATCTCC 
    GGGGACGATT CGCTGGACAC GCGTGAGGGC GTCGATCTGG TGCTCAAGTG CCGCTTCACC
    GAGCATTACG ACTCGACCGA CTTCACCTTC TACTGGGCGC GATGGACCTG CTGCCCCACA
    TTGTTCGAGA ACGTGGCCAT TGGCGATGTG CAGCTCAACT CGAATTATCG CTTGGACTTC
    CGACCCAGTC GCGGAATTTA CGATTTGCAA ATCAAAAACA CATCTTATAA TCGAGATAAT
    GGACGCTTTG AGTGCCGCAT CAAGGCGAAG GGAACGGGCG CGGATGTCCA TCAGGAGTTC
    TACAATCTGA CCGTGCTGAC GGCTCCACAT CCGCCGATGG TCACTCCCGG CAATTTAGCA
    GTGGCCACCG AAGAGAAGCC ATTGGAGTTG ACCTGCAGCA GTATTGGTGG CTCACCAGAT
    CCAATGATAA CCTGGTACCG AGAGGGCAGC ACAGTGCCAC TGCAGTCGTA TGCTCTGAAG
    GGCGGATCGA AGAACCACTA TACAAACGCC ACACTCCAAA TTGTTCCCCG ACGGGCCGAC
    GATGGCGCCA AGTACAAATG CGTCGTCTGG AATCGTGCCA TGCCCGAGGG TCATATGCTG
    GAGACCAGCG TCACGCTGAA TGTCAACTAC TATCCCCGAG TGGAGGTCGG ACCCCAGAAT
    CCTTTAAAAG TAGAACGGGA TCATGTGGCG AAACTGGACT GCAGAGTGGA TGCCAAACCG
    ATGGTTAGCA ATGTGCGATG GTCCCGAAAT GGTCAATATG TGAGTGCCAC GCCCACCCAC
    ACCATATATA GAGTAAATCG CCATCATGCC GGGAAGTACA CCTGCAGTGC GGACAACGGC
    TTGGGCAAAA CTGGCGAAAA AGATATAGTC TTGGATGTTC TATATCCGCC GATTGTGTTT
    ATCGAATCGA AAACGCACGA AGCGGAGGAA GGTGAAACGG TTTTGATTCG TTGCAATGTC
    ACCGCAAATC CATCGCCCAT CAATGTCGAA TGGCTAAAAG AAGGAGCTCC GGACTTCCGA
    TACACTGGCG AGCTCTTAAC CCTCGGTTCA GTGAGGGCTG AGCACGCCGG AAACTATATC
    TGCAGATCGG TGAACATTAT GCAACCGTTC AGTTCGAAAC GTGTAGAGGG TGTGGGCAAT
    TCCACGGTGG CTCTATTGGT TCGACATCGG CCGGGCCAGG CTTATATAAC TCCGAATAAG
    CCAGTCGTTC ATGTGGGCAA TGGTGTGACT CTAACCTGTT CAGCGAATCC GCCCGGATGG
    CCTGTACCCC AATACCGATG GTTCCGAGAT ATGGATGGTG ACATTGGAAA TACACAGAAA
    ATTTTAGCGC AAGGTCCACA GTACTCTATA CCGAAAGCTC ACTTGGGAAG CGAGGGCAAG
    TACCACTGTC ATGCGGTCAA CGAGTTGGGA ATTGGTAAAA TAGCCACCAT AATCTTAGAG
    GTACATCAGC CACCCCAGTT TCTGGCCAAA CTACAGCAAC ATATGACACG TCGGGTGGGC
    GATGTAGACT ATGCGGTCAC CTGCAGTGCC AAGGGAAAGC CTACACCGCA AATTCGATGG
    ATCAAAGATG GTACCGAGAT TCTGCCCACT CGCAAGATGT TTGATATAAG AACTACACCC
    ACGGATGCCG GTGGCGGAGT GGTGGCGGTG CAAAGTATAC TGAGATTCAG GGGTAAGGCT
    AGACCGAATG GAAACCAACT ACTTCCGAAC GATAGAGGTC TGTATACTTG CCTTTACGAA
    AACGACGTAA ATTCGGCCAA TTCTTCAATG CATCTGAGGA TCGAGCACGA ACCGATAGTC
    ATTCATCAGT ACAACAAAGT GGCCTACGAT CTTCGAGAAT CCGCTGAGGT TGTCTGCAGG
    GTTCAGGCCT ATCCGAAACC GGAGTTTCAA TGGCAATACG GCAACAATCC TTCGCCATTG
    ACCATGTCTT CGGATGGTCA TTATGAAATT AGTACGAGAA TGGAGAATAA TGATGTGTAC
    ACCTCCATTT TGAGGATAGC ACACTTGCAG CACTCTGATT ACGGAGAATA TATTTGCAGA
    GCTGTAAATC CTTTGGACAG CATAAGAGCA CCAATAAGAC TGCAACCCAA AGGATCGCCA
    GAGAAACCCA CCAATTTAAA GATCCTCGAA GTTGGTCACA ACTATGCCGT CCTAAACTGG
    ACACCGGGAT TTAATGGCGG CTTTATGAGC ACCAAATATC TGGTGAGCTA CAGGAGAGTG
    GCAACACCCA GGGAACAAAC CCTATCCGAT TGTTCAGGAA ATGGCTATAT ACCCAGCTAT
    CAGATCAGCA GTTCGTCCAG TAATTCCAAC CACGAATGGA TCGAATTCAA TTGCTTTAAG
    GAGAACCCCT GTAAACTCGC ACCATTGGAT CAACATCAGA GTTATATGTT TAAGGTTTAT
    GCCCTGAATT CGAAGGGCAC CTCGGGATAT TCGAATGAGA TATTGGCCAC CACCAAAGTC
    AGTAAAATTC CACCGCCTTT GCATGTTAGC TACGATCCCA ATTCACATGT TCTGGGCATC
    AACGTGGCCG CCACCTGTCT GTCCCTCATT GCGGTGGTGG AATCACTGGT AACCAGGGAT
    GCCACGGTGC CCATGTGGGA GATTGTGGAA ACCCTGACTT TGCTCCCGTC GGGCAGCGAA
    ACGACCTTCA AGGAGGCCAT CATCAATCAT GTGTCGCGTC CAGCCCACTA CACCACCGCC
    ACCACGTCCG GAAGGAGCCT TGGAGTGGGT GGTGGCTCCC ACCTGGGCGA GGATCGCACC
    ATGGCGTTGG CCGAGACCGC GGGACCCGGT CCAGTGGTGC GGGTCAAACT CTGTCTGAGA
    TCTAACCACG AGCACTGTGG TGCATATGCC AATGCAGAAA TTGGCAAGTC CTACATGCCG
    CACAAATCCT CCATGACGAC ATCTGCATTA GTGGCCATCA TCATCGCTTC GCTTTCTTTC
    GTCGTCTTTC TTGGTCTTCT CTACGCTTTT TGCCACTGCC GTAGGAAACA TGCCGCCAAA
    AAGGAGAGTA GCTCAGTAGG AGGAGGAGTT GGAGGAGGCA ACGCTAACGC CACGGCTAAT
    CCAGGATCTA CCGGTGCCAA AGAGTACGAC CTCGACTTGG ACGCCAGCCG AAGACCGTCG
    CTAAGTCAGG ATCCACAGCA ATCGCAACAG CAGCCACCGC CACCACCACC CTACTATCCC
    ACGGGAACTC TGGACTCCAA GGACATTGGC AATGGGAACG GCGGCATGGA GCTCACCCTC
    ACCGCCCTAC ACGATCCTGA TGAGCAGCTG AATATGCAAC AGCAGCAGCA CCACAGCAAC
    CACGGCCAGT ATCAACAGCC CAAAGCCATA CTGGGTATCT ACGGCGGAGT GGCTGGCTCT
    GGGGGCAACA ATTCCGGCGG ACAGCATCCA CATTCGAACG GCTACGGCTA TCATGTGACA
    AGTGCCATCG GCGTGGACAG CGACAGCTAT CAAGTGCTGC CCTCCGTTGC CAACTCAGCA
    GCCGGATCCC ACGGACATGG CAGTGGACAC GGACACGGAC TAGGAGCCGG AGAGTCAGGA
    CCACTTGAGG CGACACCACC AACATGCAAC ATCAGCGGCG GCAGCAGCAG CAACAGCGGC
    ATTAACCCAA TGCAGCAGCA ACATTCAGCA CGCGCCAATC TAACGAACCA ACCCACCATC
    GCCACTGCCA GCTCCACCAA CAACTACAAC AACCACTTGA ACAACACCAA CATCGCCCAC
    ACCACCAACA ACACCAACAA CTGCACCACC CTGAAACGCG GCCACTTGGG AAATAGGGAG
    AGAGAGCGGG AAAGATGCCA GGTAACCGCA GCCACGGCGG CAACAACAAC GGCACTGGCA
    ACCACCATAA CAACAACATC TCGCAATGCC AAGGCAGCAA CAACAACAAC AACACTAGCC
    ATTACAGGGT CAAGCAGCAA TTCGAACGAG AATAACTACA GTAATGCCAG GGATCTGTCG
    CAGGAGGCAT TGTGGCGCCT CCAAATGGCC ACCGCCCAGT CGCAACAGAT TTACGTGGAG
    CGACCACCGT CGGCATTCAG TGGCCTGGTC GACTATAGTG GCTATTCGCC GCACATCCCC
    ACGGTGACCA GTTCGCTCAG CCAACAGAGT TTCAGTCCAA CGCAGCAATT AGCGCCCCAC
    GAAATGCTGC AGGCCGCCCA GCGTTACGGA ACTTTGAGGA AATCCGGAAA ACAACCGCCT
    CTTCCCCCGC AGCGAAAGGA TATGCAGCAG CAGGCGAAGC CACCTCAGCA AATGGCGGAT
    ATAATACAGG ATTTGGCAAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.