Npc1b cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Npc1b
Entrez Gene ID	33072
Full Name	Niemann-Pick type C-1b
Synonyms	151,4,7,AF2/19,CG12092,Dmel\CG12092,Dmel_CG11233,NPC1b,Q256,R-9-28,R9-28,dncp1b,l(1)19Ec,l(1)2-3,l(1)R-9-28,l151,l152,lN5,lt2-14a,npc1b
General protein information	Preferred Names Niemann-Pick type C-1b Names CG12092 gene product from transcript CG12092-RA CG12092-PA NPC1b Niemann-Pick Npc1b-PA lethal (1) 19Ec section 4 section 7
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 19E4-19E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_134573.3	NP_608417.2	Niemann-Pick type C-1b

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Npc1b	NP_608417.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000579	XP_310493.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings show that NPC1b is expressed in the midgut epithelium and is essential for growth during larval development; findings suggest that NPC1b plays an early role in sterol absorption
Title: Drosophila NPC1b promotes an early step in sterol absorption from the midgut epithelium.

The following Npc1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Npc1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03240	NM_134573.3 Latest version!	Drosophila melanogaster Niemann-Pick type C-1b (Npc1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Npc1b ( NM_134573.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608417.2 Drosophila melanogaster Niemann-Pick type C-1b (Npc1b), mRNA.

Price & Availability↑

CloneID	OFa03240
Clone ID Related Accession (Same CDS sequence)	NM_134573.3
Accession Version	NM_134573.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Niemann-Pick type C-1b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_134573.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAGTGA TTTTTGCAAC TATATGGCTT ATAGCCGGAG CTTGGTCGCA AAGCGCAGAG 
CAGTTGGGAT GCATTTGGTA TGGTCAGAGT CACATGATAG GTGCCCATTG GGTGAATAAG 
GGCGACACGA ATCCAGCGAG ACCTCTGAAT AGCCCAACTT CAGAAGCCAT ATTCGCCAAG 
CGATGTCCAA TGTTGTACAA GGAGTACAAA GGTGAAAGTG GGGAGGATGA GCTCAGTCTG 
TGCTGCGATG CGGCCCAAAT CGAGACCATG GAATCTGGAT TATCACAAGC AGATGGTGTC 
TTCTCCAGAT GTCCCACCTG CACAAGAAAC ATGGCCTTGA CCGTATGCGC CATGACCTGT 
GCCAAGAACC ACACTCTCTT CTTGACCGCC TACAATGACA CAAACGATGC AGGAGTGGAC 
TATGTGAAAT ACATTGACTA TCGACTTACA GATGATACGG TATCGAAGAT CTACAACAGC 
TGCATTGGTA TACAGCACAC GCAAACCGGA CGTCCTGCCA TGGATCTGGG CTGTGGCAGC 
TACAATGCCA AGACCTGCAA CTACCGGAGA TGGTACGAGT TCATGGGCGA CGTTTCCGGC 
GACTACGTGC CGTTCCAGAT CAACTACAAA TGGTCGGAGG ACGCGGAGGA GGGTTCCAAT 
GAGATATACC TCGATCTGAG CCCCCTGAAG TGCGGCGAGA GTTACGAGGA CAGCTACGCG 
TGCGCCTGCA TCGATTGCGA GGAGTCCTGC CCGTTGACCG ATGCTCCTAC GGGTCCCGAC 
GAGCTGTGGA AGATCGCAGG ATTGTATGGA GTCACCTTTA TTCTGGCATT AATCATCGCC 
TGCGCCCTTT CGTTCTTTAT TTTCTGGGGT GCTTTTGGCA AGACATCGGC TCCTAGTGTC 
TGCATGCCCA CCCTCTTCGG CGAGTTTTTC TACCATGGCT TCCGCATCTG GGGCACCTTT 
TGTGCCAAAC ACCCGGTCAT AGTTCTGGCA CTCTGTTCCT GGGCCATCGC CGGACTGTCC 
TTCGGCATTC GGTACATGAC CATCACTACG GATCCTGTGG AACTATGGGC AGGCGAGGAA 
AGTCAGACGA GGATCGAGAA GGACTATTTC GATCAGCACT TCGGCCCGTT CTATCGCACC 
AACCAGATGT TCGTAAAGGC CGTCAATCAG ACATATTTCA CCCACGAAAC TTCCAATGGT 
GTCCTGAACT TTGGTCCAGC CTTCGAGTAT AACTTCCTAA AGGAAGTCTT CGAGCTGCAG 
GACTCTATTA TGAAATTGGG TATGGCGGAT AATGAGGGCC TCGATAAGAT CTGCTATGCC 
CCTGTCTTAA TGGCTGGTGA AACGCCCACA GTCGATCGCT GCGCAATCCA GTCGGTTTAT 
GGATATTTCC AGCACGATAT GGATCGTTTT GAGAACTCTT ATGTCGATAG CAATAACTAT 
ACCATTAACT ACCTCAATCA ATTGGAGGAT TGTCTTCGAG TCCCCATGAT GGAGGATTGC 
TTTGGAACAT TTGGCGGACC CATTGAGCCT GGAATTGCAG TCGGTGGCAT GCCTAAGGTG 
GCGGTGGGTG AGGATCCGGA CTACATGCTG GCCACCGGAC TAGTGCTCAC CTTCTTGGGT 
CGGAACTATA ACGACGAGTC CAAGCTGGAG CCGAATATGA AGTGGGAAAA GCTATTCGTG 
GACTTCTTGC GGGACTACAA GAGCGATCGA CTGGACATCG CCTACATGGC CGAGAGATCC 
ATCCAGGATG CGATTGTGGA ATTATCCGAG GGAGAAGTGA GCACTGTGGT CATCAGCTAT 
GTGGTGATGT TCGTGTACGT GGCCATAGCT TTGGGTCACA TTCGCAGCTG TCGGGGCTTC 
CTGAGGGAGT CTCGGATAAT GCTGGCCATC GGAGGCATAG TCATTGTCTT GGCATCAGTG 
GTGTGCAGCT TGGGCTTCTG GGGTTACCTC GATGTAACCA CCACAATGCT GGCGATCGAA 
GTGATTCCGT TTTTGGTGCT CGCTGTTGGA GTGGATAATA TCTTCATAAT GGTGCATACG 
TACCAGAGAC TGGATCATTC GAAATTTAAG ACCACACATG AGGCAATCGG AGAGGCCATT 
GGCCAGGTGG GTCCATCGAT ACTCCAGACG GCGGGTAGCG AAATGGCCTG CTTTGCCATC 
GGGTGTATTT CGGATATGCC CGCTGTAAAA ACCTTCGCCA TGTATGCCGC CATCGCCATT 
CTCCTGGACT TTCTGCTCCA GATCACCGCG TTTGTGGCCC TGATGGCCAT CGATGAGAAG 
CGTTACTTGG ACGGACGACT GGACATGTTG TGCTGTGTAA AGAGTGGTGG AAAGAAGATC 
AATGACGAGG ATGGCGATGG CGTGGATAGA CCCAAGGAGG TCGGCTTGCT GGAGACCTTG 
TTCAAGAACT TCTATTCCCC CTTCCTACTG TCGAAGCCCG TTAAGGTGTC CGTGCTGCTG 
ATCTTCACCG TGATTACCTG CCTTTCGCTT ATGGTGACGC CCTCTATTGA GAAGGGTCTA 
GATCAAGAGA TGTCCATGCC GAAAAACTCC CATGTGGTCA AGTATTTCCG CTACATGGTC 
GACCTGCTAG CGATGGGAGC TCCGGTCTAC TGGGTCCTCA AGCCCGGACT CAATTACTCC 
GAACCGCTGC AACAGAATCT CATATGCGGC GGAGTCGAGT GCAATAACAA CTCACTGTCC 
GTCCAGCTTT ACACGCAGGC CCAGTACCCG GAAATTACGT CATTGGCCCG ACCGGCCTCT 
TCCTGGCTGG ATGACTATAT CGATTGGCTG GCCATCAGCG ATTGCTGCAA GTACAACGTC 
ACCACCGGCG GATTTTGTTC CAGCAACTCC AAAAGTGAAG ACTGTCTGCC CTGTGAACGA 
GGATTTACTG AGAATGGCCT AAGACCTGAT GCCGAAACCT TCAACAAGTA CATTCCCTAT 
TTTCTATTCG ATCTGCCAGA TGCCGAGTGT GCCAAGGCTG GTAGAGCTTC ATACGCTGAT 
GCCGTGATCT ACACCATCGA CGATGTGGGA ATGTCCACTG TACAGGACAG CTACTTCATG 
CAATATTCCA CAACATCGAC CACCTCAGAA GAATTCTATT CGCAGCTGCG CGAAGTGCGA 
AGAATTTCTG GGGAGATAAA TGCGATGTTC AAAGAGAATA ACGTGGACGC GGAGATATTC 
GCGTACTGTG TTTTCTACAT CTACTACGAG CAGTACCTGA CGATCTGGGG AGATGCAATG 
TTTTCGCTAG GAATGTCCTT GGTGGCCATA TTCTTGGTCA CTCTATTGAT CACTGGTCTG 
GACATCACAT CAACGTTCAT CGTTCTCTTC ATGGTGATCT GCATATTGAT CAATATGCTG 
GGCATGATGT GGGCGTGGAG CATTAATCTT AATGCCATAT CGCTGGTGAA TCTGGTGGTT 
TGCGTGGGCA TTGGAGTTGA GTTTGTGGCG CATATAGTGC GGTCCTTTAA AAGGGCGGAA 
GGAACTGCCC AGGAGCGGGC ACGCCACTCC CTTAATGTAA CCGGAAGTAG TGTCCTCTCC 
GGCATAACTC TCACCAAATT CGCGGGCATC GTTGTCCTGG GCTTCTCCAA TTCGCAAATC 
TTCCAGGTGT TCTACTTCCG GATGTATCTG GGCATTGTCC TTATTGGAGC AGCCCACGGC 
TTGATCCTGT TGCCTGTTCT CCTCAGTCTC CTGGGTCCAC CACAAAAATT GGCCAGAAGC 
TCAGGAGCCG AGCCCACCGC ATCTATCACC ATCACAACCA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608417.2
CDS	85..3849
Translation	MKVIFATIWLIAGAWSQSAEQLGCIWYGQSHMIGAHWVNKGDTNPARPLNSPTSEAIFAKRCPMLYKEYKGESGEDELSLCCDAAQIETMESGLSQADGVFSRCPTCTRNMALTVCAMTCAKNHTLFLTAYNDTNDAGVDYVKYIDYRLTDDTVSKIYNSCIGIQHTQTGRPAMDLGCGSYNAKTCNYRRWYEFMGDVSGDYVPFQINYKWSEDAEEGSNEIYLDLSPLKCGESYEDSYACACIDCEESCPLTDAPTGPDELWKIAGLYGVTFILALIIACALSFFIFWGAFGKTSAPSVCMPTLFGEFFYHGFRIWGTFCAKHPVIVLALCSWAIAGLSFGIRYMTITTDPVELWAGEESQTRIEKDYFDQHFGPFYRTNQMFVKAVNQTYFTHETSNGVLNFGPAFEYNFLKEVFELQDSIMKLGMADNEGLDKICYAPVLMAGETPTVDRCAIQSVYGYFQHDMDRFENSYVDSNNYTINYLNQLEDCLRVPMMEDCFGTFGGPIEPGIAVGGMPKVAVGEDPDYMLATGLVLTFLGRNYNDESKLEPNMKWEKLFVDFLRDYKSDRLDIAYMAERSIQDAIVELSEGEVSTVVISYVVMFVYVAIALGHIRSCRGFLRESRIMLAIGGIVIVLASVVCSLGFWGYLDVTTTMLAIEVIPFLVLAVGVDNIFIMVHTYQRLDHSKFKTTHEAIGEAIGQVGPSILQTAGSEMACFAIGCISDMPAVKTFAMYAAIAILLDFLLQITAFVALMAIDEKRYLDGRLDMLCCVKSGGKKINDEDGDGVDRPKEVGLLETLFKNFYSPFLLSKPVKVSVLLIFTVITCLSLMVTPSIEKGLDQEMSMPKNSHVVKYFRYMVDLLAMGAPVYWVLKPGLNYSEPLQQNLICGGVECNNNSLSVQLYTQAQYPEITSLARPASSWLDDYIDWLAISDCCKYNVTTGGFCSSNSKSEDCLPCERGFTENGLRPDAETFNKYIPYFLFDLPDAECAKAGRASYADAVIYTIDDVGMSTVQDSYFMQYSTTSTTSEEFYSQLREVRRISGEINAMFKENNVDAEIFAYCVFYIYYEQYLTIWGDAMFSLGMSLVAIFLVTLLITGLDITSTFIVLFMVICILINMLGMMWAWSINLNAISLVNLVVCVGIGVEFVAHIVRSFKRAEGTAQERARHSLNVTGSSVLSGITLTKFAGIVVLGFSNSQIFQVFYFRMYLGIVLIGAAHGLILLPVLLSLLGPPQKLARSSGAEPTASITITTN

Target ORF information:

RefSeq Version	NM_134573.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Niemann-Pick type C-1b (Npc1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134573.3

ORF Insert Sequence:

ATGAAAGTGA TTTTTGCAAC TATATGGCTT ATAGCCGGAG CTTGGTCGCA AAGCGCAGAG 
CAGTTGGGAT GCATTTGGTA TGGTCAGAGT CACATGATAG GTGCCCATTG GGTGAATAAG 
GGCGACACGA ATCCAGCGAG ACCTCTGAAT AGCCCAACTT CAGAAGCCAT ATTCGCCAAG 
CGATGTCCAA TGTTGTACAA GGAGTACAAA GGTGAAAGTG GGGAGGATGA GCTCAGTCTG 
TGCTGCGATG CGGCCCAAAT CGAGACCATG GAATCTGGAT TATCACAAGC AGATGGTGTC 
TTCTCCAGAT GTCCCACCTG CACAAGAAAC ATGGCCTTGA CCGTATGCGC CATGACCTGT 
GCCAAGAACC ACACTCTCTT CTTGACCGCC TACAATGACA CAAACGATGC AGGAGTGGAC 
TATGTGAAAT ACATTGACTA TCGACTTACA GATGATACGG TATCGAAGAT CTACAACAGC 
TGCATTGGTA TACAGCACAC GCAAACCGGA CGTCCTGCCA TGGATCTGGG CTGTGGCAGC 
TACAATGCCA AGACCTGCAA CTACCGGAGA TGGTACGAGT TCATGGGCGA CGTTTCCGGC 
GACTACGTGC CGTTCCAGAT CAACTACAAA TGGTCGGAGG ACGCGGAGGA GGGTTCCAAT 
GAGATATACC TCGATCTGAG CCCCCTGAAG TGCGGCGAGA GTTACGAGGA CAGCTACGCG 
TGCGCCTGCA TCGATTGCGA GGAGTCCTGC CCGTTGACCG ATGCTCCTAC GGGTCCCGAC 
GAGCTGTGGA AGATCGCAGG ATTGTATGGA GTCACCTTTA TTCTGGCATT AATCATCGCC 
TGCGCCCTTT CGTTCTTTAT TTTCTGGGGT GCTTTTGGCA AGACATCGGC TCCTAGTGTC 
TGCATGCCCA CCCTCTTCGG CGAGTTTTTC TACCATGGCT TCCGCATCTG GGGCACCTTT 
TGTGCCAAAC ACCCGGTCAT AGTTCTGGCA CTCTGTTCCT GGGCCATCGC CGGACTGTCC 
TTCGGCATTC GGTACATGAC CATCACTACG GATCCTGTGG AACTATGGGC AGGCGAGGAA 
AGTCAGACGA GGATCGAGAA GGACTATTTC GATCAGCACT TCGGCCCGTT CTATCGCACC 
AACCAGATGT TCGTAAAGGC CGTCAATCAG ACATATTTCA CCCACGAAAC TTCCAATGGT 
GTCCTGAACT TTGGTCCAGC CTTCGAGTAT AACTTCCTAA AGGAAGTCTT CGAGCTGCAG 
GACTCTATTA TGAAATTGGG TATGGCGGAT AATGAGGGCC TCGATAAGAT CTGCTATGCC 
CCTGTCTTAA TGGCTGGTGA AACGCCCACA GTCGATCGCT GCGCAATCCA GTCGGTTTAT 
GGATATTTCC AGCACGATAT GGATCGTTTT GAGAACTCTT ATGTCGATAG CAATAACTAT 
ACCATTAACT ACCTCAATCA ATTGGAGGAT TGTCTTCGAG TCCCCATGAT GGAGGATTGC 
TTTGGAACAT TTGGCGGACC CATTGAGCCT GGAATTGCAG TCGGTGGCAT GCCTAAGGTG 
GCGGTGGGTG AGGATCCGGA CTACATGCTG GCCACCGGAC TAGTGCTCAC CTTCTTGGGT 
CGGAACTATA ACGACGAGTC CAAGCTGGAG CCGAATATGA AGTGGGAAAA GCTATTCGTG 
GACTTCTTGC GGGACTACAA GAGCGATCGA CTGGACATCG CCTACATGGC CGAGAGATCC 
ATCCAGGATG CGATTGTGGA ATTATCCGAG GGAGAAGTGA GCACTGTGGT CATCAGCTAT 
GTGGTGATGT TCGTGTACGT GGCCATAGCT TTGGGTCACA TTCGCAGCTG TCGGGGCTTC 
CTGAGGGAGT CTCGGATAAT GCTGGCCATC GGAGGCATAG TCATTGTCTT GGCATCAGTG 
GTGTGCAGCT TGGGCTTCTG GGGTTACCTC GATGTAACCA CCACAATGCT GGCGATCGAA 
GTGATTCCGT TTTTGGTGCT CGCTGTTGGA GTGGATAATA TCTTCATAAT GGTGCATACG 
TACCAGAGAC TGGATCATTC GAAATTTAAG ACCACACATG AGGCAATCGG AGAGGCCATT 
GGCCAGGTGG GTCCATCGAT ACTCCAGACG GCGGGTAGCG AAATGGCCTG CTTTGCCATC 
GGGTGTATTT CGGATATGCC CGCTGTAAAA ACCTTCGCCA TGTATGCCGC CATCGCCATT 
CTCCTGGACT TTCTGCTCCA GATCACCGCG TTTGTGGCCC TGATGGCCAT CGATGAGAAG 
CGTTACTTGG ACGGACGACT GGACATGTTG TGCTGTGTAA AGAGTGGTGG AAAGAAGATC 
AATGACGAGG ATGGCGATGG CGTGGATAGA CCCAAGGAGG TCGGCTTGCT GGAGACCTTG 
TTCAAGAACT TCTATTCCCC CTTCCTACTG TCGAAGCCCG TTAAGGTGTC CGTGCTGCTG 
ATCTTCACCG TGATTACCTG CCTTTCGCTT ATGGTGACGC CCTCTATTGA GAAGGGTCTA 
GATCAAGAGA TGTCCATGCC GAAAAACTCC CATGTGGTCA AGTATTTCCG CTACATGGTC 
GACCTGCTAG CGATGGGAGC TCCGGTCTAC TGGGTCCTCA AGCCCGGACT CAATTACTCC 
GAACCGCTGC AACAGAATCT CATATGCGGC GGAGTCGAGT GCAATAACAA CTCACTGTCC 
GTCCAGCTTT ACACGCAGGC CCAGTACCCG GAAATTACGT CATTGGCCCG ACCGGCCTCT 
TCCTGGCTGG ATGACTATAT CGATTGGCTG GCCATCAGCG ATTGCTGCAA GTACAACGTC 
ACCACCGGCG GATTTTGTTC CAGCAACTCC AAAAGTGAAG ACTGTCTGCC CTGTGAACGA 
GGATTTACTG AGAATGGCCT AAGACCTGAT GCCGAAACCT TCAACAAGTA CATTCCCTAT 
TTTCTATTCG ATCTGCCAGA TGCCGAGTGT GCCAAGGCTG GTAGAGCTTC ATACGCTGAT 
GCCGTGATCT ACACCATCGA CGATGTGGGA ATGTCCACTG TACAGGACAG CTACTTCATG 
CAATATTCCA CAACATCGAC CACCTCAGAA GAATTCTATT CGCAGCTGCG CGAAGTGCGA 
AGAATTTCTG GGGAGATAAA TGCGATGTTC AAAGAGAATA ACGTGGACGC GGAGATATTC 
GCGTACTGTG TTTTCTACAT CTACTACGAG CAGTACCTGA CGATCTGGGG AGATGCAATG 
TTTTCGCTAG GAATGTCCTT GGTGGCCATA TTCTTGGTCA CTCTATTGAT CACTGGTCTG 
GACATCACAT CAACGTTCAT CGTTCTCTTC ATGGTGATCT GCATATTGAT CAATATGCTG 
GGCATGATGT GGGCGTGGAG CATTAATCTT AATGCCATAT CGCTGGTGAA TCTGGTGGTT 
TGCGTGGGCA TTGGAGTTGA GTTTGTGGCG CATATAGTGC GGTCCTTTAA AAGGGCGGAA 
GGAACTGCCC AGGAGCGGGC ACGCCACTCC CTTAATGTAA CCGGAAGTAG TGTCCTCTCC 
GGCATAACTC TCACCAAATT CGCGGGCATC GTTGTCCTGG GCTTCTCCAA TTCGCAAATC 
TTCCAGGTGT TCTACTTCCG GATGTATCTG GGCATTGTCC TTATTGGAGC AGCCCACGGC 
TTGATCCTGT TGCCTGTTCT CCTCAGTCTC CTGGGTCCAC CACAAAAATT GGCCAGAAGC 
TCAGGAGCCG AGCCCACCGC ATCTATCACC ATCACAACCA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Npc1b cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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