THADA cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	THADA
Entrez Gene ID	33001
Full Name	CG15618 gene product from transcript CG15618-RB
Synonyms	Dmel\CG15618
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 18F4-18F4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001258841.1	NP_001245770.1	THADA, isoform C
NM_134517.3	NP_608361.3	THADA, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following THADA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THADA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03179	NM_001258841.1 Latest version!	Drosophila melanogaster THADA, transcript variant C (THADA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa02190	NM_134517.3 Latest version!	Drosophila melanogaster THADA, transcript variant B (THADA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

THADA ( NM_001258841.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245770.1 Drosophila melanogaster THADA, transcript variant C (THADA), mRNA.

Price & Availability↑

CloneID	OFa03179
Clone ID Related Accession (Same CDS sequence)	NM_001258841.1
Accession Version	NM_001258841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	THADA, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACGATC TGAACCTGCG CGTGGCCGCC TTGAAGGTGT GTGCTCACCC CAAGAGATTT 
GCCGAGCTGC GCGACGCCCT TGTGCCACTG CCCAACACGT GGATTGCCGC GCCACATGAC 
TTTGTGCTCC AGTTCGCGGC GGCCAAGAGC AGTGCGGAGC AGGTGCAGGT GGTCAAGGAT 
GTCTTTTACG GGGAGCAGGC GCAAGATCAT GAAGATGTAG CCCACTTCCT GGCGGATCTC 
CTGCTGGCCA GTCCCCTGAA GCACGCCGTG CGCAACCAGC TGACCAAGTA CGACTCTTCT 
CGGACAATGC GCTGGCCAAA CAGAATGCGT CGCCACATCG CAGGCACTCG AAGGAGCACC 
TGCTGGAGGC TCTGCAGCAG TCGCTCGGCG AAATGGCCAA CAGTCTCGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245770.1
CDS	67..480
Translation	MNDLNLRVAALKVCAHPKRFAELRDALVPLPNTWIAAPHDFVLQFAAAKSSAEQVQVVKDVFYGEQAQDHEDVAHFLADLLLASPLKHAVRNQLTKYDSSRTMRWPNRMRRHIAGTRRSTCWRLCSSRSAKWPTVSQ

Target ORF information:

RefSeq Version	NM_001258841.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster THADA, transcript variant C (THADA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258841.1

ORF Insert Sequence:

ATGAACGATC TGAACCTGCG CGTGGCCGCC TTGAAGGTGT GTGCTCACCC CAAGAGATTT 
GCCGAGCTGC GCGACGCCCT TGTGCCACTG CCCAACACGT GGATTGCCGC GCCACATGAC 
TTTGTGCTCC AGTTCGCGGC GGCCAAGAGC AGTGCGGAGC AGGTGCAGGT GGTCAAGGAT 
GTCTTTTACG GGGAGCAGGC GCAAGATCAT GAAGATGTAG CCCACTTCCT GGCGGATCTC 
CTGCTGGCCA GTCCCCTGAA GCACGCCGTG CGCAACCAGC TGACCAAGTA CGACTCTTCT 
CGGACAATGC GCTGGCCAAA CAGAATGCGT CGCCACATCG CAGGCACTCG AAGGAGCACC 
TGCTGGAGGC TCTGCAGCAG TCGCTCGGCG AAATGGCCAA CAGTCTCGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THADA ( NM_134517.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608361.3 Drosophila melanogaster THADA, transcript variant B (THADA), mRNA.

Price & Availability↑

CloneID	OFa02190
Clone ID Related Accession (Same CDS sequence)	NM_134517.3
Accession Version	NM_134517.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	THADA, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 26, 2009 this sequence version replaced NM_134517.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACGATC TGAACCTGCG CGTGGCCGCC TTGAAGGTGT GTGCTCACCC CAAGAGATTT 
GCCGAGCTGC GCGACGCCCT TGTGCCACTG CCCAACACGT GGATTGCCGC GCCACATGAC 
TTTGTGCTCC AGTTCGCGGC GGCCAAGAGC AGTGCGGAGC AGGTGCAGGT GGTCAAGGAT 
GTCTTTTACG GGGAGCAGGC GCAAGATCAT GAAGATGTAG CCCACTTCCT GGCGGATCTC 
CTGCTGGCCA GTCCCCTGAA GCACGCCGTG CGCAACCAGC TGACCAAACT CTTCTCGGAC 
AATGCGCTGG CCAAACAGAA TGCGTCGCCA CATCGCAGGC ACTCGAAGGA GCACCTGCTG 
GAGGCTCTGC AGCAGTCGCT CGGCGAAATG GCCAACAGTC TCGCAGTGAT CACGCCGCCT 
GTCAGCCACG AGCGCACCAA CGATGTCTTC GTGTCCGCCA ACGCCTGCCT GCAGAACTTT 
CCCTTTGGAC GGGAGGCCCT GGGCAAACAG GTGCATCGCT TTGCGCCGCT GCTAACTACC 
GCCTTGGAGC GCTACTGGGC GGACATTTGT GACCCTACGC TGGAACTCTC GCCAACGCGT 
CGCAACGAGC TCTACTTGTA CGTGCAGAAT GCGCTGCGGT TCTTGGTTAG TCTGCTGGCC 
GAGTGGAGCG ACAAGTTGCG CCTGTTCGAG GATCAGCGGT TTCCGGGCAC GTCGAATGCG 
GTGGCCCAAA AGGTGGCTCG CTACTACGAC ACTCCGTGGG ACGTACGCTC CATTGCCGCC 
CTGCTGATCG GTCACCTGGC TCGGTTTTCC GGAACATTCA AGGCGTATGT CGAAGACTGC 
TCGAGGCCAA AGGCAGAGCA GGATGTTCCC ATACAGATGG CCGCCCTGCT CGTCCTGCGG 
CCCGTCGATT ATACAGAAAA CGCTACACTA GCACTGGCTA TTCTGAAGAG GATCGTGGCC 
GTCAGCGAGC TGAAAAGCAC CGTGACCAAT CTGCTTGTCT TCCTTTCCAA GCATCTGTTC 
ATTTACTCCA AATCGCTGGG CGAGATGCAC GCCCATCTTC CTGACGACCA GAAGTTGCTA 
TACCAACGGA TCTTGGCACA GCTGCAGGTG TTCGCCCTGC AGAATATCTC CAGTGACACG 
GACTCCGTGC GCCACATGAG CAGCGCCCTG CTGCACCAGG TGCTGCAGCA CGCCCAGGCC 
GCCGGCCAGG AGGAGCTCTT CCAAGTCGTC TATCGCCAGT TTGAAGATCG TGCTGCATAC 
CTAAATGCCA GTTGCATGGC ACTGGAGCAG CTGGTTGCGG TGGCCGGAGT CAGCAAGTCC 
ATCGAGAACT GTCCTTCGCT GTTCGGAGTC ATATTCCCGC GCCATCTGGG CTGTGAGGAT 
TGTGTGGATG CCCTGTTCAA AGCCATGATG GTATCGGCCC ACAAGACGGA ACCCTTCGCC 
GAGTGGCAGA GCCGGTGGTT CGGTCTGCTT TTGGCGGCAA TCCGCGTGCC GGAGAAGCGC 
CGCCAGGTCA TTGAGGAGCT GATCGCACAG GCCGTACAGC TGGAGCCAAC GAGACTGGCG 
CAAGTGCTCC TGCCAGACGA CCGGCTGCCG CTTAGCTGCA AGCTGGCGGC GATTCTGGGC 
GTGAGGCAAC TCAGCGCCAG GCGGCAGAAT CTGTTGCGTG GCATGAAAGA GGAGGTGGAA 
CAGGCCCTCA TCGGACTGGA CGACCACACG CGCCTACTGG CGCTGCGATT TCTGGTGGAG 
ACGCCGCGTC CCAGCGAACT TTTAAATGCC GACCAAATGG GCGCCATTGA ACTTTACGTG 
CGGCACAATG CCAACAATCC TAGCGCCCAT CTGCGGCAGC TAGGCTACGG TCTGCTGCAG 
AAGGCCCTCA AGAGAGTCCA TTTCGGCCTG GTCGAGTACC GGAAGAGCCG CACGCCGGCG 
TCGCAGGAGG TGCTCCAGTT CCTAATACGG CTTATCCGCA CTCTCGCGCA GAATCTGTTT 
CCCACGGCGA ACTACGGACG ACGCTGGCTT TCGCTGCGCT TGCTGCGCGA TTGCCTGGAG 
TTAAGCGAGA TGGTGGGCAT AACGTTCAGC GAGTTGGGAA TAGAGCTGCC CACAGAGGCA 
CTGATGGCCT GCTTGGGCGA CAGCTACGAG CACAACAAAG TGCTGGCCGC TCAGCTGCTG 
GAGCGATTTC AATCCCATTC CCTCTTCAAG CCGGATGAGA TGATCGAGCT GCTGCTCTCC 
CTCAGGCCAT CGGACTCCGC CACCGGAGCC TTCCAGCTGC AGGTCTATTG CAAAGCCAGT 
CGAGTGCAAA GCGAGATGCC CACGCCAACG CACGGTGGCA CAATCCACGA GCCGCTTACC 
TTCAGGGCAT TGCAGTGGTG CCTGCAGCAC TTGAGGGAGG GCCTGCGTCT CGCCCAGCTT 
GATCTTGGCG AGGCGGCCAA GCTGAACCCT TTGTACGGCC TGCTTTTTGC CAGCCGGCAT 
CTGCTGCAGC AACTCAAACT CAAGGAACTG GCGAAAGAGC CTCAGTGGAG GCAGTACATA 
GACGAGCTAG TGACCATGTG CCTGGCAGTC AGCAGCGTAG TGCTGCCTGT GGTCAGCAGC 
GCTTCCCCCG AAGGCCACCT GCCCGAAACC TGCGACCAGG AGACGGATCA GCCGCTGACC 
AACGTGCTCG ACCGTCAGTT GAGCCGCGAG GAACTGCTGC AGGTGCGCAC GACGCCCCAG 
ATGATACTGC TGTGCGCTTG GCGCAGCAGC AAGGAGGTGT GCCTCATCCT GGGCGAGCTT 
GTCCAGCGCG CACCACTGGA GGAGGAGGAG GATGAGGAGC AGCAGCAGCA GCAGGGCGAC 
TTCCTGCTCA GCCGTGCTCA GCTGGAGGCC ATCGGGGAGC ATTTCCTGCA ACTGCTGGCC 
GAGACGAAGC ATCGCGGCGC CTTCGAGCAG GCCTACGTGG GATTCACGAT GCTGTGCCGC 
CGCTTCTGGC ACAGCGAGTC GGTGCGTCTC AACCAGCTGC CCGGCCAGTG GGTCGACGAG 
GCCATGGCGA TGGTCAGCGG CCAGGAGGAG TGGGCAGGCA AGGGTGCCCG CCTGTGCGCT 
ACGCGCCGCA GTGCCGGAAT GCCCTTCATG CTGCAGGCGC TGGTCGGCAC GGAGCTCAAG 
CTGGGCACCC ATGCCACTCT CTATCGCTGC ATGAACCGCC TGCTCGAGGT GTGTGAGCGC 
CGGACAGGTG GAGCCGCTGG TATCACCGCC CGCAGCCACG CCCTCAACAT CATGCGCGCC 
CTCTTCCGCA GCAGCGAACT GGCCGAGCTG GTCACCGAGT TCATGGCCCG CGGCATTCAG 
TGCGCCCTCG ACGGACTGCT GCTCGCGGAG GAGTGGGCGG AGCGCAATAG CGCCACTCTG 
CTGCTGGCCG CCCTCATCGT TCGCGTCTTT GGCGTGGAGC GTGCCCGCCT GGAGACCGGT 
GAGCTGCACG TGCGCAACCG CATGACGGGA CGCATCTTCT TTACGCGCTA TCCGCAGCTC 
TTCGACTACT TCCACGCCGC CCTGCAGCGC GAGTCGGAGC AGATGGACGC CGGCGGCGGG 
GGCAGCGAGA ACGCGAGCGG GAAGCGGCGG CAGGCGGTCC AGCTGGAGGC CATGCTGCTG 
ATGCTCAGCC GGCTGTATCC GTCCTCGCTG GAGGGCGCGG AGTCAACCTT AAATCTCAGC 
GAGTTTGTGC CCTTCCTGAT AAGGATCTGC CACTCCCACG ATCTGATGAC ACGTGAAATG 
GCTGCCCTGG TGGTGGCCAA CTTTGTCACC CAGGAACAAG CGCTGGCCGA GATCAGGCGC 
ATCGTCGTGG AACTTAAGGC ACTGCAGCTA CGGCTAAAGA ACACCGAGGC AGCTAACACC 
AAGCTAAACA CCAATGTCCT GCACGGCCAG TTGCTTCTGC TGCTGCATCT ACACCGCTTG 
GTTCGCTGGA CACGTCCTTC GTTGACCAGA ATGCAGCTAC ACACCCTCGC CGAACTGGCA 
GCTCCGCTCC TACAGCACGA CGCGTGTGCA TTCAGTGCGC TGGTGGCGGT GATGGTGGCT 
GCCATGGAGG ATGCCGTGGA GCCCGGCTTG CTGGATTTCC AGCTGCTGGA GCAGATTGGC 
GTCGTTTATC TGCTGAATCA TAAGGAGGTC CAAAGCCGTT GCCAGCAGCT GGGTATCTCC 
AACCGTTTCT ATCAGATCTT TGGCCTCCAT CTGCACCGTC TACGCGGCAT TTCCCAGGGA 
ATAGTGCTGC ACATCGTCGA GGATCTGGCC GAAACCATCT GGGCTCTGGA CGAGCTGAAA 
GTGGAGCTGT GGCTGTACAT TCTTCTACAG AGATCCCTAA GCGAGCAGAA TTCGCTGGTC 
AGTGAGCAGG ATATCGAGCA CTTTGAGTTT TCACGCGACA TTCGCCGATA TTTCGAGACC 
CTTAGTCGTG AGCAGCGGGA GGAGGTGGGC CAGGAACTGT ATGAATCGCC GGCTGTTCGC 
TCGAGCGTTC TGCACATGAT GCACATGATC AAAAGCTCAA AGAACAGCTG TTGGAGCCTT 
CAGCTGGCCG GTCGCCTGGC CGCCTTGCAA ACCCTGCTTC GAGATCCGGG ACTGGAACTG 
AATCAGTTAG TCCAGCGCTG CTCAGAGGAG CACTCCACCC ACCAGGAGGC GGGCTTGCTG 
CTCGGCCTGC GTAGGCTGAT CGGCGAAAGC AAGATGCTGG AGCGAAAGCA CTGGCTGCCC 
ATGCTGAACT ACGCGCAGCG ACTTGTGCAT CCTGGCCAAC CGGTCTATCT TCGCCATCAG 
GCTGCCGAGC TGTGCGATTC GCTGGCTCGC AATCACCTGC GTGATCAACT GGTTGCAGGT 
ACCACAGATG TGGACATTGG GTTGGTCGGA CGCTTTAGTG GACTGGTGCT GCTGCTGCTG 
CACGACGACG CGGAGTGGGT GCGTCATCGG GCTGTCCAGC TGGTGTGCGG CGCTGGACTG 
CGGACGAGGA GTGGGGCTGG ACAGGAGCAA GAGCAGTCTG CCATGGCGCC ACTCATCCTG 
CCCAGTGCCT TGATACCACC ATTCTTGGAC ACCATGATCG GTAAGCTGAC CTTCGATGAC 
TTCAACATGG TACAGCGCCT GGTTGACATC ATCGCTGAGC CCTTCACTAC CGCCGATGCA 
ATGGAGCTGT TCGACAAGCA GGAGAACAAC CATTACTGCG AAAGGAACCA CGTTTTAACC 
GAGCTATGGG ACGCTCGTGG ACGAGCAGAT CCACGTGCAA GAACACCAAC AATACCGACG 
GGGTATCAAA TATTTAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608361.3
CDS	67..5307
Translation	MNDLNLRVAALKVCAHPKRFAELRDALVPLPNTWIAAPHDFVLQFAAAKSSAEQVQVVKDVFYGEQAQDHEDVAHFLADLLLASPLKHAVRNQLTKLFSDNALAKQNASPHRRHSKEHLLEALQQSLGEMANSLAVITPPVSHERTNDVFVSANACLQNFPFGREALGKQVHRFAPLLTTALERYWADICDPTLELSPTRRNELYLYVQNALRFLVSLLAEWSDKLRLFEDQRFPGTSNAVAQKVARYYDTPWDVRSIAALLIGHLARFSGTFKAYVEDCSRPKAEQDVPIQMAALLVLRPVDYTENATLALAILKRIVAVSELKSTVTNLLVFLSKHLFIYSKSLGEMHAHLPDDQKLLYQRILAQLQVFALQNISSDTDSVRHMSSALLHQVLQHAQAAGQEELFQVVYRQFEDRAAYLNASCMALEQLVAVAGVSKSIENCPSLFGVIFPRHLGCEDCVDALFKAMMVSAHKTEPFAEWQSRWFGLLLAAIRVPEKRRQVIEELIAQAVQLEPTRLAQVLLPDDRLPLSCKLAAILGVRQLSARRQNLLRGMKEEVEQALIGLDDHTRLLALRFLVETPRPSELLNADQMGAIELYVRHNANNPSAHLRQLGYGLLQKALKRVHFGLVEYRKSRTPASQEVLQFLIRLIRTLAQNLFPTANYGRRWLSLRLLRDCLELSEMVGITFSELGIELPTEALMACLGDSYEHNKVLAAQLLERFQSHSLFKPDEMIELLLSLRPSDSATGAFQLQVYCKASRVQSEMPTPTHGGTIHEPLTFRALQWCLQHLREGLRLAQLDLGEAAKLNPLYGLLFASRHLLQQLKLKELAKEPQWRQYIDELVTMCLAVSSVVLPVVSSASPEGHLPETCDQETDQPLTNVLDRQLSREELLQVRTTPQMILLCAWRSSKEVCLILGELVQRAPLEEEEDEEQQQQQGDFLLSRAQLEAIGEHFLQLLAETKHRGAFEQAYVGFTMLCRRFWHSESVRLNQLPGQWVDEAMAMVSGQEEWAGKGARLCATRRSAGMPFMLQALVGTELKLGTHATLYRCMNRLLEVCERRTGGAAGITARSHALNIMRALFRSSELAELVTEFMARGIQCALDGLLLAEEWAERNSATLLLAALIVRVFGVERARLETGELHVRNRMTGRIFFTRYPQLFDYFHAALQRESEQMDAGGGGSENASGKRRQAVQLEAMLLMLSRLYPSSLEGAESTLNLSEFVPFLIRICHSHDLMTREMAALVVANFVTQEQALAEIRRIVVELKALQLRLKNTEAANTKLNTNVLHGQLLLLLHLHRLVRWTRPSLTRMQLHTLAELAAPLLQHDACAFSALVAVMVAAMEDAVEPGLLDFQLLEQIGVVYLLNHKEVQSRCQQLGISNRFYQIFGLHLHRLRGISQGIVLHIVEDLAETIWALDELKVELWLYILLQRSLSEQNSLVSEQDIEHFEFSRDIRRYFETLSREQREEVGQELYESPAVRSSVLHMMHMIKSSKNSCWSLQLAGRLAALQTLLRDPGLELNQLVQRCSEEHSTHQEAGLLLGLRRLIGESKMLERKHWLPMLNYAQRLVHPGQPVYLRHQAAELCDSLARNHLRDQLVAGTTDVDIGLVGRFSGLVLLLLHDDAEWVRHRAVQLVCGAGLRTRSGAGQEQEQSAMAPLILPSALIPPFLDTMIGKLTFDDFNMVQRLVDIIAEPFTTADAMELFDKQENNHYCERNHVLTELWDARGRADPRARTPTIPTGYQIFK

Target ORF information:

RefSeq Version	NM_134517.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster THADA, transcript variant B (THADA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134517.3

ORF Insert Sequence:

ATGAACGATC TGAACCTGCG CGTGGCCGCC TTGAAGGTGT GTGCTCACCC CAAGAGATTT 
GCCGAGCTGC GCGACGCCCT TGTGCCACTG CCCAACACGT GGATTGCCGC GCCACATGAC 
TTTGTGCTCC AGTTCGCGGC GGCCAAGAGC AGTGCGGAGC AGGTGCAGGT GGTCAAGGAT 
GTCTTTTACG GGGAGCAGGC GCAAGATCAT GAAGATGTAG CCCACTTCCT GGCGGATCTC 
CTGCTGGCCA GTCCCCTGAA GCACGCCGTG CGCAACCAGC TGACCAAACT CTTCTCGGAC 
AATGCGCTGG CCAAACAGAA TGCGTCGCCA CATCGCAGGC ACTCGAAGGA GCACCTGCTG 
GAGGCTCTGC AGCAGTCGCT CGGCGAAATG GCCAACAGTC TCGCAGTGAT CACGCCGCCT 
GTCAGCCACG AGCGCACCAA CGATGTCTTC GTGTCCGCCA ACGCCTGCCT GCAGAACTTT 
CCCTTTGGAC GGGAGGCCCT GGGCAAACAG GTGCATCGCT TTGCGCCGCT GCTAACTACC 
GCCTTGGAGC GCTACTGGGC GGACATTTGT GACCCTACGC TGGAACTCTC GCCAACGCGT 
CGCAACGAGC TCTACTTGTA CGTGCAGAAT GCGCTGCGGT TCTTGGTTAG TCTGCTGGCC 
GAGTGGAGCG ACAAGTTGCG CCTGTTCGAG GATCAGCGGT TTCCGGGCAC GTCGAATGCG 
GTGGCCCAAA AGGTGGCTCG CTACTACGAC ACTCCGTGGG ACGTACGCTC CATTGCCGCC 
CTGCTGATCG GTCACCTGGC TCGGTTTTCC GGAACATTCA AGGCGTATGT CGAAGACTGC 
TCGAGGCCAA AGGCAGAGCA GGATGTTCCC ATACAGATGG CCGCCCTGCT CGTCCTGCGG 
CCCGTCGATT ATACAGAAAA CGCTACACTA GCACTGGCTA TTCTGAAGAG GATCGTGGCC 
GTCAGCGAGC TGAAAAGCAC CGTGACCAAT CTGCTTGTCT TCCTTTCCAA GCATCTGTTC 
ATTTACTCCA AATCGCTGGG CGAGATGCAC GCCCATCTTC CTGACGACCA GAAGTTGCTA 
TACCAACGGA TCTTGGCACA GCTGCAGGTG TTCGCCCTGC AGAATATCTC CAGTGACACG 
GACTCCGTGC GCCACATGAG CAGCGCCCTG CTGCACCAGG TGCTGCAGCA CGCCCAGGCC 
GCCGGCCAGG AGGAGCTCTT CCAAGTCGTC TATCGCCAGT TTGAAGATCG TGCTGCATAC 
CTAAATGCCA GTTGCATGGC ACTGGAGCAG CTGGTTGCGG TGGCCGGAGT CAGCAAGTCC 
ATCGAGAACT GTCCTTCGCT GTTCGGAGTC ATATTCCCGC GCCATCTGGG CTGTGAGGAT 
TGTGTGGATG CCCTGTTCAA AGCCATGATG GTATCGGCCC ACAAGACGGA ACCCTTCGCC 
GAGTGGCAGA GCCGGTGGTT CGGTCTGCTT TTGGCGGCAA TCCGCGTGCC GGAGAAGCGC 
CGCCAGGTCA TTGAGGAGCT GATCGCACAG GCCGTACAGC TGGAGCCAAC GAGACTGGCG 
CAAGTGCTCC TGCCAGACGA CCGGCTGCCG CTTAGCTGCA AGCTGGCGGC GATTCTGGGC 
GTGAGGCAAC TCAGCGCCAG GCGGCAGAAT CTGTTGCGTG GCATGAAAGA GGAGGTGGAA 
CAGGCCCTCA TCGGACTGGA CGACCACACG CGCCTACTGG CGCTGCGATT TCTGGTGGAG 
ACGCCGCGTC CCAGCGAACT TTTAAATGCC GACCAAATGG GCGCCATTGA ACTTTACGTG 
CGGCACAATG CCAACAATCC TAGCGCCCAT CTGCGGCAGC TAGGCTACGG TCTGCTGCAG 
AAGGCCCTCA AGAGAGTCCA TTTCGGCCTG GTCGAGTACC GGAAGAGCCG CACGCCGGCG 
TCGCAGGAGG TGCTCCAGTT CCTAATACGG CTTATCCGCA CTCTCGCGCA GAATCTGTTT 
CCCACGGCGA ACTACGGACG ACGCTGGCTT TCGCTGCGCT TGCTGCGCGA TTGCCTGGAG 
TTAAGCGAGA TGGTGGGCAT AACGTTCAGC GAGTTGGGAA TAGAGCTGCC CACAGAGGCA 
CTGATGGCCT GCTTGGGCGA CAGCTACGAG CACAACAAAG TGCTGGCCGC TCAGCTGCTG 
GAGCGATTTC AATCCCATTC CCTCTTCAAG CCGGATGAGA TGATCGAGCT GCTGCTCTCC 
CTCAGGCCAT CGGACTCCGC CACCGGAGCC TTCCAGCTGC AGGTCTATTG CAAAGCCAGT 
CGAGTGCAAA GCGAGATGCC CACGCCAACG CACGGTGGCA CAATCCACGA GCCGCTTACC 
TTCAGGGCAT TGCAGTGGTG CCTGCAGCAC TTGAGGGAGG GCCTGCGTCT CGCCCAGCTT 
GATCTTGGCG AGGCGGCCAA GCTGAACCCT TTGTACGGCC TGCTTTTTGC CAGCCGGCAT 
CTGCTGCAGC AACTCAAACT CAAGGAACTG GCGAAAGAGC CTCAGTGGAG GCAGTACATA 
GACGAGCTAG TGACCATGTG CCTGGCAGTC AGCAGCGTAG TGCTGCCTGT GGTCAGCAGC 
GCTTCCCCCG AAGGCCACCT GCCCGAAACC TGCGACCAGG AGACGGATCA GCCGCTGACC 
AACGTGCTCG ACCGTCAGTT GAGCCGCGAG GAACTGCTGC AGGTGCGCAC GACGCCCCAG 
ATGATACTGC TGTGCGCTTG GCGCAGCAGC AAGGAGGTGT GCCTCATCCT GGGCGAGCTT 
GTCCAGCGCG CACCACTGGA GGAGGAGGAG GATGAGGAGC AGCAGCAGCA GCAGGGCGAC 
TTCCTGCTCA GCCGTGCTCA GCTGGAGGCC ATCGGGGAGC ATTTCCTGCA ACTGCTGGCC 
GAGACGAAGC ATCGCGGCGC CTTCGAGCAG GCCTACGTGG GATTCACGAT GCTGTGCCGC 
CGCTTCTGGC ACAGCGAGTC GGTGCGTCTC AACCAGCTGC CCGGCCAGTG GGTCGACGAG 
GCCATGGCGA TGGTCAGCGG CCAGGAGGAG TGGGCAGGCA AGGGTGCCCG CCTGTGCGCT 
ACGCGCCGCA GTGCCGGAAT GCCCTTCATG CTGCAGGCGC TGGTCGGCAC GGAGCTCAAG 
CTGGGCACCC ATGCCACTCT CTATCGCTGC ATGAACCGCC TGCTCGAGGT GTGTGAGCGC 
CGGACAGGTG GAGCCGCTGG TATCACCGCC CGCAGCCACG CCCTCAACAT CATGCGCGCC 
CTCTTCCGCA GCAGCGAACT GGCCGAGCTG GTCACCGAGT TCATGGCCCG CGGCATTCAG 
TGCGCCCTCG ACGGACTGCT GCTCGCGGAG GAGTGGGCGG AGCGCAATAG CGCCACTCTG 
CTGCTGGCCG CCCTCATCGT TCGCGTCTTT GGCGTGGAGC GTGCCCGCCT GGAGACCGGT 
GAGCTGCACG TGCGCAACCG CATGACGGGA CGCATCTTCT TTACGCGCTA TCCGCAGCTC 
TTCGACTACT TCCACGCCGC CCTGCAGCGC GAGTCGGAGC AGATGGACGC CGGCGGCGGG 
GGCAGCGAGA ACGCGAGCGG GAAGCGGCGG CAGGCGGTCC AGCTGGAGGC CATGCTGCTG 
ATGCTCAGCC GGCTGTATCC GTCCTCGCTG GAGGGCGCGG AGTCAACCTT AAATCTCAGC 
GAGTTTGTGC CCTTCCTGAT AAGGATCTGC CACTCCCACG ATCTGATGAC ACGTGAAATG 
GCTGCCCTGG TGGTGGCCAA CTTTGTCACC CAGGAACAAG CGCTGGCCGA GATCAGGCGC 
ATCGTCGTGG AACTTAAGGC ACTGCAGCTA CGGCTAAAGA ACACCGAGGC AGCTAACACC 
AAGCTAAACA CCAATGTCCT GCACGGCCAG TTGCTTCTGC TGCTGCATCT ACACCGCTTG 
GTTCGCTGGA CACGTCCTTC GTTGACCAGA ATGCAGCTAC ACACCCTCGC CGAACTGGCA 
GCTCCGCTCC TACAGCACGA CGCGTGTGCA TTCAGTGCGC TGGTGGCGGT GATGGTGGCT 
GCCATGGAGG ATGCCGTGGA GCCCGGCTTG CTGGATTTCC AGCTGCTGGA GCAGATTGGC 
GTCGTTTATC TGCTGAATCA TAAGGAGGTC CAAAGCCGTT GCCAGCAGCT GGGTATCTCC 
AACCGTTTCT ATCAGATCTT TGGCCTCCAT CTGCACCGTC TACGCGGCAT TTCCCAGGGA 
ATAGTGCTGC ACATCGTCGA GGATCTGGCC GAAACCATCT GGGCTCTGGA CGAGCTGAAA 
GTGGAGCTGT GGCTGTACAT TCTTCTACAG AGATCCCTAA GCGAGCAGAA TTCGCTGGTC 
AGTGAGCAGG ATATCGAGCA CTTTGAGTTT TCACGCGACA TTCGCCGATA TTTCGAGACC 
CTTAGTCGTG AGCAGCGGGA GGAGGTGGGC CAGGAACTGT ATGAATCGCC GGCTGTTCGC 
TCGAGCGTTC TGCACATGAT GCACATGATC AAAAGCTCAA AGAACAGCTG TTGGAGCCTT 
CAGCTGGCCG GTCGCCTGGC CGCCTTGCAA ACCCTGCTTC GAGATCCGGG ACTGGAACTG 
AATCAGTTAG TCCAGCGCTG CTCAGAGGAG CACTCCACCC ACCAGGAGGC GGGCTTGCTG 
CTCGGCCTGC GTAGGCTGAT CGGCGAAAGC AAGATGCTGG AGCGAAAGCA CTGGCTGCCC 
ATGCTGAACT ACGCGCAGCG ACTTGTGCAT CCTGGCCAAC CGGTCTATCT TCGCCATCAG 
GCTGCCGAGC TGTGCGATTC GCTGGCTCGC AATCACCTGC GTGATCAACT GGTTGCAGGT 
ACCACAGATG TGGACATTGG GTTGGTCGGA CGCTTTAGTG GACTGGTGCT GCTGCTGCTG 
CACGACGACG CGGAGTGGGT GCGTCATCGG GCTGTCCAGC TGGTGTGCGG CGCTGGACTG 
CGGACGAGGA GTGGGGCTGG ACAGGAGCAA GAGCAGTCTG CCATGGCGCC ACTCATCCTG 
CCCAGTGCCT TGATACCACC ATTCTTGGAC ACCATGATCG GTAAGCTGAC CTTCGATGAC 
TTCAACATGG TACAGCGCCT GGTTGACATC ATCGCTGAGC CCTTCACTAC CGCCGATGCA 
ATGGAGCTGT TCGACAAGCA GGAGAACAAC CATTACTGCG AAAGGAACCA CGTTTTAACC 
GAGCTATGGG ACGCTCGTGG ACGAGCAGAT CCACGTGCAA GAACACCAAC AATACCGACG 
GGGTATCAAA TATTTAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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THADA cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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