Cngl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cngl
Entrez Gene ID	32468
Full Name	Cyclic nucleotide-gated ion channel-like
Synonyms	CG18210,CG43395,CG9176,CNGL,Dmel\CG43395,Dmel_CG18210,Dmel_CG9176,cngl,dmCNG3,mdcds_55060
General protein information	Preferred Names Cyclic nucleotide-gated ion channel-like Names CG43395 gene product from transcript CG43395-RD CG43395-PD CG43395-PE CG43395-PF CG43395-PI CG43395-PJ CNG channel-like Cngl-PD Cngl-PE Cngl-PF Cngl-PI Cngl-PJ cng-like cyclic nucleotide-gated ion channel-like
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 13C1-13C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001258762.3	NP_001245691.1	cyclic nucleotide-gated ion channel-like, isoform E
NM_167441.5	NP_727848.3	cyclic nucleotide-gated ion channel-like, isoform J
NM_132794.5	NP_573022.2	cyclic nucleotide-gated ion channel-like, isoform F
NM_001370020.1	NP_001356908.1	cyclic nucleotide-gated ion channel-like, isoform K
NM_001272642.2	NP_001259571.2	cyclic nucleotide-gated ion channel-like, isoform I
NM_001258761.3	NP_001245690.1	cyclic nucleotide-gated ion channel-like, isoform D

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	cngl	NP_001245691.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003349	XP_003436450.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cngl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cngl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01680	NM_132794.5 Latest version!	Drosophila melanogaster cyclic nucleotide-gated ion channel-like, transcript variant F (Cngl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa21480	NM_167441.5 Latest version!	Drosophila melanogaster cyclic nucleotide-gated ion channel-like, transcript variant J (Cngl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
OFa21481	NM_001272642.2 Latest version!	Drosophila melanogaster cyclic nucleotide-gated ion channel-like, transcript variant I (Cngl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
OFa87676	NM_001370020.1 Latest version!	Drosophila melanogaster cyclic nucleotide-gated ion channel-like, transcript variant K (Cngl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFa02727	NM_001258761.3 Latest version!	Drosophila melanogaster cyclic nucleotide-gated ion channel-like, transcript variant D (Cngl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa02728	NM_001258762.3 Latest version!	Drosophila melanogaster cyclic nucleotide-gated ion channel-like, transcript variant E (Cngl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cngl ( NM_132794.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573022.2 Drosophila melanogaster cyclic nucleotide-gated ion channel-like, transcript variant F (Cngl), mRNA.

Price & Availability↑

CloneID	OFa01680
Clone ID Related Accession (Same CDS sequence)	NM_132794.5
Accession Version	NM_132794.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cyclic nucleotide-gated ion channel-like, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132794.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAACTGA GCGTGAGCGC GTATCGGGCG CATGCATCCG CACACAAGAA GATCCTCTCC 
AGCGGCTATC ACTCACAGCT CAACTACGGC AGCACTGGAG CGGCAGCGGC ATCCTCCTCG 
TCGTCGGGGG CCACTGCAAC GGGCCTATAT ACGATGGAAG CGCACGAGCA CGAAGCGGGC 
AAGTTTGTCA AGGATGTGGC CCGTCAGGTG CACGACTGCA ACAGCGACAC GGATGTCATC 
AGTCCCACGG GCACCAGTAT CTCCGGTGGC GGAGGAGGCG GCGGAGCAGG CGGTGCCGGC 
GGATCCGGTG GTGCTGGCGG ACACAGCGAC GGTGGCTATC ACAAGCGGCA CCACCACAAG 
ATGGTCCGCG ATGACGACAA CAACAGTGCG GCGCACTCAT CGGACAGCAA GAGTCCCAGC 
CAGCGATCCG GCAGCTCGTC GCTAACCAGT GGGCAACTGC ACCACTGCGG CTGCCCCACC 
ACCACTAGTT TAAGGTATAC TGAGAGGGAT AGACTTATAC TGACGCCGGC ACAACCGCAT 
CAACATCCGC ATACGCATCC GCATCCGTAT CCGCATCCGC ACCAGGCCCG TCAGCGGCGC 
CAGGTGCGCC TCCATGTGAA CAGTCCGTCC TCCGACCAGA GCGCCAGCAC GCCGGTGGGT 
CCCAGGCCAT TGCCAGCGAT GAAGACCCTC TCGTCGTTCA TCAGCGAGCA TCCCGAGGAG 
CTTGTGGCAG CGGAGGATCA GTCCCGGAGT GGCGCCTTCG CCACCTCCTC GGCATCCGCA 
TCCGCAGCCG CCACTGCCAA TGCCTCCAAT GGCCAGCAGC AGACAACGAA GTGGAAGAGC 
CTGCGAGCCG TGATGGCCTA CTACTGCTCC CTGCGGCGCA TCAAGAGGAA CGTTGCCACC 
CACAAGTCCC ACTTTCAGTG TTCCGTAATT GCGTATCCGC GTTTTGCTAG CGTTAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573022.2
CDS	2250..3209
Translation	MKLSVSAYRAHASAHKKILSSGYHSQLNYGSTGAAAASSSSSGATATGLYTMEAHEHEAGKFVKDVARQVHDCNSDTDVISPTGTSISGGGGGGGAGGAGGSGGAGGHSDGGYHKRHHHKMVRDDDNNSAAHSSDSKSPSQRSGSSSLTSGQLHHCGCPTTTSLRYTERDRLILTPAQPHQHPHTHPHPYPHPHQARQRRQVRLHVNSPSSDQSASTPVGPRPLPAMKTLSSFISEHPEELVAAEDQSRSGAFATSSASASAAATANASNGQQQTTKWKSLRAVMAYYCSLRRIKRNVATHKSHFQCSVIAYPRFASVN

Target ORF information:

RefSeq Version	NM_132794.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cyclic nucleotide-gated ion channel-like, transcript variant F (Cngl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132794.5

ORF Insert Sequence:

ATGAAACTGA GCGTGAGCGC GTATCGGGCG CATGCATCCG CACACAAGAA GATCCTCTCC 
AGCGGCTATC ACTCACAGCT CAACTACGGC AGCACTGGAG CGGCAGCGGC ATCCTCCTCG 
TCGTCGGGGG CCACTGCAAC GGGCCTATAT ACGATGGAAG CGCACGAGCA CGAAGCGGGC 
AAGTTTGTCA AGGATGTGGC CCGTCAGGTG CACGACTGCA ACAGCGACAC GGATGTCATC 
AGTCCCACGG GCACCAGTAT CTCCGGTGGC GGAGGAGGCG GCGGAGCAGG CGGTGCCGGC 
GGATCCGGTG GTGCTGGCGG ACACAGCGAC GGTGGCTATC ACAAGCGGCA CCACCACAAG 
ATGGTCCGCG ATGACGACAA CAACAGTGCG GCGCACTCAT CGGACAGCAA GAGTCCCAGC 
CAGCGATCCG GCAGCTCGTC GCTAACCAGT GGGCAACTGC ACCACTGCGG CTGCCCCACC 
ACCACTAGTT TAAGGTATAC TGAGAGGGAT AGACTTATAC TGACGCCGGC ACAACCGCAT 
CAACATCCGC ATACGCATCC GCATCCGTAT CCGCATCCGC ACCAGGCCCG TCAGCGGCGC 
CAGGTGCGCC TCCATGTGAA CAGTCCGTCC TCCGACCAGA GCGCCAGCAC GCCGGTGGGT 
CCCAGGCCAT TGCCAGCGAT GAAGACCCTC TCGTCGTTCA TCAGCGAGCA TCCCGAGGAG 
CTTGTGGCAG CGGAGGATCA GTCCCGGAGT GGCGCCTTCG CCACCTCCTC GGCATCCGCA 
TCCGCAGCCG CCACTGCCAA TGCCTCCAAT GGCCAGCAGC AGACAACGAA GTGGAAGAGC 
CTGCGAGCCG TGATGGCCTA CTACTGCTCC CTGCGGCGCA TCAAGAGGAA CGTTGCCACC 
CACAAGTCCC ACTTTCAGTG TTCCGTAATT GCGTATCCGC GTTTTGCTAG CGTTAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cngl ( NM_167441.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727848.3 Drosophila melanogaster cyclic nucleotide-gated ion channel-like, transcript variant J (Cngl), mRNA.

Price & Availability↑

CloneID	OFa21480
Clone ID Related Accession (Same CDS sequence)	NM_167441.5
Accession Version	NM_167441.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cyclic nucleotide-gated ion channel-like, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167441.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAACTGA GCGTGAGCGC GTATCGGGCG CATGCATCCG CACACAAGAA GATCCTCTCC 
AGCGGCTATC ACTCACAGCT CAACTACGGC AGCACTGGAG CGGCAGCGGC ATCCTCCTCG 
TCGTCGGGGG CCACTGCAAC GGGCCTATAT ACGATGGAAG CGCACGAGCA CGAAGCGGGC 
AAGTTTGTCA AGGATGTGGC CCGTCAGGTG CACGACTGCA ACAGCGACAC GGATGTCATC 
AGTCCCACGG GCACCAGTAT CTCCGGTGGC GGAGGAGGCG GCGGAGCAGG CGGTGCCGGC 
GGATCCGGTG GTGCTGGCGG ACACAGCGAC GGTGGCTATC ACAAGCGGCA CCACCACAAG 
ATGGTCCGCG ATGACGACAA CAACAGTGCG GCGCACTCAT CGGACAGCAA GAGTCCCAGC 
CAGCGGAAAT CGCGTATCGG AGATGGAGCT TCAGATCCAG ATTCCGATTG GACACGATCG 
AACCAACGCT GGATGAAGCT ACGCACCACC GTGCAGATCT CATCGGCGAT ACAGAAGAAA 
CCACCGCTCA AGCGCGAAGA CTCCTTCCTG AAGCGTTTCT CGACTAGACA GATACCCGAA 
ACACAGGAAA CTGTTGAAGA TACGGGTTCA GAAAGTGCCT CTGGTGACGT CGACAAGAGT 
GTTAAACGAC GGCGACGCTA TCTGCAGAAA CGACGATCCG TTGTTAATCC AGATGAAAAT 
TTTTACTTCT ATTGGTTAAT GATGTTAACT GTATGTGTTC TATATAATCT ATGGACCCTA 
ATTGTGAGGC AGAGCTTTCC TGAACTGCAG CAATCTGTGC CCACGTTTTG GCTCATCTGC 
GATTCGATGA CAGATGTTGT ATTTATCCTA GATATAATAG TTCAATTACG CACAGGCTAT 
CTGGAGCAGG GCCTAATGGT ATACGACGAC AGGAAGCTGG CCTGTCACTA CGTTCACTCG 
CGCGACTTTA TCTTCGATAT GATAGCGCTG ATACCATTGG ATTTGCTGCA GCTCAAGATG 
GGCACACATC CGCTCTTGCG TTTTACGCGC TTTTTTAAAG TTTATCGATC TGTGAGATTT 
TATTACATCG TAGAAAGTAG AACAGTTTGG CCAAATTTAT GGCGTGTTGT TAACCTAATT 
CATATCCTGT TAATACTGGC ACACTGGTTC GGTTGTTTCT ATTTTTTACT CTCCGAGGCG 
GAGGGCTTTC AGGGCGACTG GGTGTATCCG TATCGACCCG GGGACTATGC CACCCTAACG 
CGAAAGTATC TGGGCAGCCT GTACTGGTCG ACCCTGACAC TGACGACCAT TGGGGACCTG 
CCCACACCCG AAACGAATGC AGAACCGAAC TTTTCGGTGG ACGGCCCACG ATCAATTCCG 
CGTCGTGGCA TTAAATCGCG CCTACTTCAA TTTGGCTCTA GCTATGGATA TATTTTTACG 
ATCGTTAGCT ATTTGATTGG TGTTTTTATC TTCGCCACCA TTGTGGGCCA AGTGGGCAAT 
GTGATAACGA ACCGAAATGC GAATCGCCTG GAGTTCGAGC GCCTTCTGGA TGGGGCCAAG 
ACCTATATGC GGCACCACAA GGTGCCCGGA GGGATGAAGC GGCGCGTGCT GCGATGGTAC 
GACTATAGCT GGTCCCGCGG CAGGATACAG GGTGGCGGTG ACATCAATAC CGCACTGGGC 
CTCCTGCCCG ACAAGCTGAA AACCGAATTG GCCTTACATG TCAACCTGAG CGTTCTGAAG 
AAGGTGACCA TATTCCAAGA GTGCCAGCCC GAGTTCCTTC ACGATCTCGT GCTCAAGATG 
AAGGCCTACA TCTTTACGCC GGGCGACTCC ATTTGCCGTA AGGGCGAGGT GGCCCGCGAG 
ATGTTCATCA TTGCCGATGG CATTCTGGAG GTGCTGAGCG AGACGGGCAA GGTCCTAACC 
ACCATGAAGG CTGGCGATTT TTTTGGCGAA ATCGGCATCC TCAATCTGGA CGGGCTTAAC 
AAACGCACAG CGGATGTGCG CTCCGTGGGC TACTCGGAGC TCTTCTCTCT GTCCCGCGAG 
GATGTCCTGG CGGCCATGAA GGACTACCCC GATGCCCAGG AGATTCTGCA GACCCTGGGC 
CGCAAGCGGC TAATGGAGGT GCGTTGCGTG AACAAAAAGT ACGCCAAGGC GCAGAGTGAC 
AAGGAGGCGG CCGCCTATGC GGCAGCTCAT CCGCACCACC ATCAGAGCCA TCACCAGGGT 
CAGGTGCACC AGAGCGACAG CAGCGAGAAC AGTGCCTCCA AGAAGATCGT GGACAAGCTG 
AAGCACGACG TCAAGGGATT CCGCAACGTG CTGAAGAAGT CCAGGACTTC GCGCAAAAGC 
GATGAATCCT TGGAGATGCA GCCACTGCAC AACACCTCGC CGCGCGGCAG CAAGATCCTG 
CTGAAGCGCA TGTCACGCGT TCGATCGGAC GAAAAGGATG CGGATAGCGC CGAGGCCAAG 
GACGAGTTGC ACGACAAGAC GCCCAGTCCG ATTGGGGCGG GACTGCCATT GCTGCAGCGC 
CTCCGGCTCC TCAAGGAGAA GCAGGAATCG ATCCAGGAGG AGCCCGAACG CGAGTTCAGC 
GAAGGCTTTC CCCTGATCCA GCGACTCCAG CAGTTGAAAA TTAAGAATGA GCCGCAGGCA 
AACGCCGCCG AACCCGGCGT CGTCATGGTC AACACACCGC CCAACATTAG CAGCAAAATT 
GATTCGCATT TCGCTGCCAG CCAGGCAGGT GCATCGCATC CTGGCCCAAA TGGCGGAGCC 
ATCGGCATCT CGGCGCCGGG ACAATCGCTG ACGGTGGCCC AGATCAAGCC CATCATGAAG 
GTGTCCTTCA AGCAGAAGAT CCAGCAGATG CAGGGTGGCG GCGGAGGAGC CAGCAGTTCA 
CCGGGTCCAA GCACGGGAGC CATAGCCAAG AAGGAGCCAC CCAAGTCACT GGCTCTGGTG 
GCCAAGCACG CTACATCCGA GGATCCCGTC GCCGCAGCCG GATTGGGTTT GGGATCTGGT 
TCTAGTTCCG GCATTGCCAC CATATCGAAG AGGGTAGTCA AACCTCAAAG CCATCGGATG 
GCTACGCCGC TGCAATCCGA CACCGACACC GATGGCACGC CCATCAAACC GTGGTCCAAG 
CTGAAACTGG CCACCCTGAT GTCCTCCAGC TACACGAGCT TAACCAACTG TTCGCCGGAT 
GATCTGGCCT CACCGCTGAA GAACTACTCG CTGAGCAACA TACCGCAGCA GATGGAGTCG 
CATACGCGAC CACGTCACCA TAGCGCCAGA AGTAGTTCGA GATCACCGCG TCATGGACAC 
CACCATCACC ATCATCATGG CCACCACCAG CACGGACAGC TTCAGGGGCC AAGCTCCACG 
TGCACCAACG GCTCCACGAC CAGCCAGGTG AACCAGGTGA CGGCGAGTGC AAAGCGTGAT 
TGCTTGCGTC TCCAGAAGCC GGAACAGCAC TTGCCCGACG GCGAACTGAC CGAACTGGGC 
GGCAATGGAC GGAGGAAGCT CTACCAAAGC GTGTTCGATC TGTCGCCGGA ATACTGCGGA 
CTGCCGTTTG TCAAGCGATT GAAGATCCTC AATGAGCGCC AGAAGCTTGC GGAACTGGAG 
CGGGCATTGC AAACCCGTAG CTTTAGCCTC GACTGCTCCA AGTCCGGGCC GGATAGCAAG 
GTGCCCATAA CGGAGTCCTT GTATCGATGT TATAGCGACA CTTCCGGCAT CTACTCGCAG 
TTCCTCAGCA CGTACGAATC AACCACCAGC TCCACCTCGA TATCCACCAA TACTTCCGCC 
TCTGCATCGG CATCCGCCTC CGCATCCGCA TCCGCATCGG ATAGCAACTC CAGGCAATTG 
CAATACGTAC CCCTGCCACT CAGTCCCGAA TCGAATGAGA CCATGGAGCG GCGCAAGCTG 
AAGAGCATAC TCAAGAAACT CCAGATGGGC GGTGGTCAGC AGGAGGAGGC GGGGCCGGGC 
ACGGGAACGG GAGCGGGAGC GGTAATGGGA ACGGGGTCAA AGGTCAACTC CAGTGCCAGC 
CAAAAGGGGC AGGGCCTGCC GGCGGAGCCG ACCTTGGAAG GCCCGCCCGC CAGTGCCAAG 
GAGGAGGAAT CTCCCTTCGG CGGTGCTGCG GCGATCGGTT CGGTGAATAA TGCTTGCGGT 
AACGGTAACG GTAACGGTAG CGTTAGTGGT AACGGTAACG GTAGCGGTAC GGTAAACGAT 
AGCAGTGCTA ATTCGAGTTC GCATCCGAGC AATAACGGCA ACAGTGCGTA TACGTGTCCT 
CCTCCACCGT CAGTGGCTTT TCCGTTTCCG GTACCCATTG CACCAGCATC CGGCTCAGTT 
CCACTACCAT TGGAGCCCGG AGTGTCGAAT GTCTGGTCGC CGACGTTGAC GTTGGTAACG 
CCCACGAATT CGCCGCAGGA GAGCTTCGCG TTTCCTGCCC AACTGCAGGG GGAGTTGGGC 
GGACACGGAA CCGGGCGCGG ACTCGTTGGT GGCGTTGGCA GCGCCTCAGC GTCGATGTTG 
CCAGCGGGAG CGGGAGCAAG TGCATCCGCA TCCGCATCCA CGTCCACGTC CGCCTCGTAT 
GTCGTCGAGT GCTCAGCGTC GTCGAATCAA ATCCTCCATG CCCAGTCCAC CACTGATTTG 
AATTTGAATT TGCAGCTCAG GCAGAACACG ACCAGTTCGA TGGGCGGACC GGGTCCAGCG 
CAACGGATTG AAGGCTTCCC GGAGGCACAG GACTACTTCA ATCAGATCCT GAACGGCATC 
AATCACGTGA TCAAGACGCA CATGAACGAG ATGCACTCCA AGTTCGAGAC GCAATTCTCC 
AGCATGGCTG GTGAGGTGCG ACGACGCGAC GCTATCATTG CCCAGCTGCA GCTCAAGCTG 
CGCTCCATAG AGGGCAAGTC CTCGGCGGCA CCGACATCCT CATCCTCCTC CGCACTGGTT 
CTGCCCATCA GCCGGCGCCA GAAGAAGATG CCCCAGCTGT CGGTGGACGA CGATGAGCCG 
CCGGAGGAGG AGGACAACAG CAGTTCGGGT TCTTCGGCGG AACTTTTGTT TATGCGAGGT 
GACTCCCTGG ACACGGTGTT CACCTCATCG CCGCCCATTC AAGGGGGCAG GCGCAGCACA 
TCGCCGGGTC CTAGTCGTCA TCATGCGGCC TCGGCTAACG CCCTGAACTG TCCGAGCAGC 
TACTACGGAG GACCCGGATC CCTGAGCTCA CGCCACGCCC AGAGCCACCC CAGCTTCTAT 
TCCGGCCAGG GAAATGGACG GAACAGCGGG CGCAGCAGTG GATATCGCGA GTGGAGCGGC 
GCAGCGGAGA GCGGACACCT GGTCAGTGGG AGTGGATCGA GCGGCGCGGT CATGGTGGCC 
GATGTCACGG GCAATCTGGC CCGCCTGTCG GACAGCGTGA TCCTCGACAT TGGCGAGAGT 
TCCAGTTCGA GCTCCTCGTC CAGCAAGATC AACATGGCCG AAGTGGATGA CGACGAGGAG 
GAGGAGGATC CGAGGGTGCG GGATGCTCGA AACGAGGAGG ACATAGACGA TGGCGAAGGT 
GGTTTGGCGA GCCACAACGA CTGGGAGGTG CAGATGCTGG CCGCCGAGAT GGAACGGCAG 
GAACGGAAGC GTGGTCACTC CCTATCCGAC AATCTGGGCG AACTGAAGCA CTGCAGCACG 
TTTCTGCGGC GACGCCGGAA GTTCAGCGAC ACGGAAACGG AATTTAGTGA GACGGACATG 
GAGGAGCAGT TGGCCAGATC GGGCAGCGGC CCGATGGAGC CAAGCGGATC GGCACCATCT 
AGCTCTGGAG CCGGTGGCTC CACATCCGCT GGACATCAGA GCGGCAGCCA GCGACCGAGG 
GCCTCCAGTC TGGATCAGTT CAATCTGCGC TACGGCATCG GGCGCGGAAT CTTTAAAGCA 
ATGAGCATCG ATCGTGATAA AGACAAGCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727848.3
CDS	2250..8162
Translation	MKLSVSAYRAHASAHKKILSSGYHSQLNYGSTGAAAASSSSSGATATGLYTMEAHEHEAGKFVKDVARQVHDCNSDTDVISPTGTSISGGGGGGGAGGAGGSGGAGGHSDGGYHKRHHHKMVRDDDNNSAAHSSDSKSPSQRKSRIGDGASDPDSDWTRSNQRWMKLRTTVQISSAIQKKPPLKREDSFLKRFSTRQIPETQETVEDTGSESASGDVDKSVKRRRRYLQKRRSVVNPDENFYFYWLMMLTVCVLYNLWTLIVRQSFPELQQSVPTFWLICDSMTDVVFILDIIVQLRTGYLEQGLMVYDDRKLACHYVHSRDFIFDMIALIPLDLLQLKMGTHPLLRFTRFFKVYRSVRFYYIVESRTVWPNLWRVVNLIHILLILAHWFGCFYFLLSEAEGFQGDWVYPYRPGDYATLTRKYLGSLYWSTLTLTTIGDLPTPETNAEPNFSVDGPRSIPRRGIKSRLLQFGSSYGYIFTIVSYLIGVFIFATIVGQVGNVITNRNANRLEFERLLDGAKTYMRHHKVPGGMKRRVLRWYDYSWSRGRIQGGGDINTALGLLPDKLKTELALHVNLSVLKKVTIFQECQPEFLHDLVLKMKAYIFTPGDSICRKGEVAREMFIIADGILEVLSETGKVLTTMKAGDFFGEIGILNLDGLNKRTADVRSVGYSELFSLSREDVLAAMKDYPDAQEILQTLGRKRLMEVRCVNKKYAKAQSDKEAAAYAAAHPHHHQSHHQGQVHQSDSSENSASKKIVDKLKHDVKGFRNVLKKSRTSRKSDESLEMQPLHNTSPRGSKILLKRMSRVRSDEKDADSAEAKDELHDKTPSPIGAGLPLLQRLRLLKEKQESIQEEPEREFSEGFPLIQRLQQLKIKNEPQANAAEPGVVMVNTPPNISSKIDSHFAASQAGASHPGPNGGAIGISAPGQSLTVAQIKPIMKVSFKQKIQQMQGGGGGASSSPGPSTGAIAKKEPPKSLALVAKHATSEDPVAAAGLGLGSGSSSGIATISKRVVKPQSHRMATPLQSDTDTDGTPIKPWSKLKLATLMSSSYTSLTNCSPDDLASPLKNYSLSNIPQQMESHTRPRHHSARSSSRSPRHGHHHHHHHGHHQHGQLQGPSSTCTNGSTTSQVNQVTASAKRDCLRLQKPEQHLPDGELTELGGNGRRKLYQSVFDLSPEYCGLPFVKRLKILNERQKLAELERALQTRSFSLDCSKSGPDSKVPITESLYRCYSDTSGIYSQFLSTYESTTSSTSISTNTSASASASASASASASDSNSRQLQYVPLPLSPESNETMERRKLKSILKKLQMGGGQQEEAGPGTGTGAGAVMGTGSKVNSSASQKGQGLPAEPTLEGPPASAKEEESPFGGAAAIGSVNNACGNGNGNGSVSGNGNGSGTVNDSSANSSSHPSNNGNSAYTCPPPPSVAFPFPVPIAPASGSVPLPLEPGVSNVWSPTLTLVTPTNSPQESFAFPAQLQGELGGHGTGRGLVGGVGSASASMLPAGAGASASASASTSTSASYVVECSASSNQILHAQSTTDLNLNLQLRQNTTSSMGGPGPAQRIEGFPEAQDYFNQILNGINHVIKTHMNEMHSKFETQFSSMAGEVRRRDAIIAQLQLKLRSIEGKSSAAPTSSSSSALVLPISRRQKKMPQLSVDDDEPPEEEDNSSSGSSAELLFMRGDSLDTVFTSSPPIQGGRRSTSPGPSRHHAASANALNCPSSYYGGPGSLSSRHAQSHPSFYSGQGNGRNSGRSSGYREWSGAAESGHLVSGSGSSGAVMVADVTGNLARLSDSVILDIGESSSSSSSSSKINMAEVDDDEEEEDPRVRDARNEEDIDDGEGGLASHNDWEVQMLAAEMERQERKRGHSLSDNLGELKHCSTFLRRRRKFSDTETEFSETDMEEQLARSGSGPMEPSGSAPSSSGAGGSTSAGHQSGSQRPRASSLDQFNLRYGIGRGIFKAMSIDRDKDKL

Target ORF information:

RefSeq Version	NM_167441.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cyclic nucleotide-gated ion channel-like, transcript variant J (Cngl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167441.5

ORF Insert Sequence:

ATGAAACTGA GCGTGAGCGC GTATCGGGCG CATGCATCCG CACACAAGAA GATCCTCTCC 
AGCGGCTATC ACTCACAGCT CAACTACGGC AGCACTGGAG CGGCAGCGGC ATCCTCCTCG 
TCGTCGGGGG CCACTGCAAC GGGCCTATAT ACGATGGAAG CGCACGAGCA CGAAGCGGGC 
AAGTTTGTCA AGGATGTGGC CCGTCAGGTG CACGACTGCA ACAGCGACAC GGATGTCATC 
AGTCCCACGG GCACCAGTAT CTCCGGTGGC GGAGGAGGCG GCGGAGCAGG CGGTGCCGGC 
GGATCCGGTG GTGCTGGCGG ACACAGCGAC GGTGGCTATC ACAAGCGGCA CCACCACAAG 
ATGGTCCGCG ATGACGACAA CAACAGTGCG GCGCACTCAT CGGACAGCAA GAGTCCCAGC 
CAGCGGAAAT CGCGTATCGG AGATGGAGCT TCAGATCCAG ATTCCGATTG GACACGATCG 
AACCAACGCT GGATGAAGCT ACGCACCACC GTGCAGATCT CATCGGCGAT ACAGAAGAAA 
CCACCGCTCA AGCGCGAAGA CTCCTTCCTG AAGCGTTTCT CGACTAGACA GATACCCGAA 
ACACAGGAAA CTGTTGAAGA TACGGGTTCA GAAAGTGCCT CTGGTGACGT CGACAAGAGT 
GTTAAACGAC GGCGACGCTA TCTGCAGAAA CGACGATCCG TTGTTAATCC AGATGAAAAT 
TTTTACTTCT ATTGGTTAAT GATGTTAACT GTATGTGTTC TATATAATCT ATGGACCCTA 
ATTGTGAGGC AGAGCTTTCC TGAACTGCAG CAATCTGTGC CCACGTTTTG GCTCATCTGC 
GATTCGATGA CAGATGTTGT ATTTATCCTA GATATAATAG TTCAATTACG CACAGGCTAT 
CTGGAGCAGG GCCTAATGGT ATACGACGAC AGGAAGCTGG CCTGTCACTA CGTTCACTCG 
CGCGACTTTA TCTTCGATAT GATAGCGCTG ATACCATTGG ATTTGCTGCA GCTCAAGATG 
GGCACACATC CGCTCTTGCG TTTTACGCGC TTTTTTAAAG TTTATCGATC TGTGAGATTT 
TATTACATCG TAGAAAGTAG AACAGTTTGG CCAAATTTAT GGCGTGTTGT TAACCTAATT 
CATATCCTGT TAATACTGGC ACACTGGTTC GGTTGTTTCT ATTTTTTACT CTCCGAGGCG 
GAGGGCTTTC AGGGCGACTG GGTGTATCCG TATCGACCCG GGGACTATGC CACCCTAACG 
CGAAAGTATC TGGGCAGCCT GTACTGGTCG ACCCTGACAC TGACGACCAT TGGGGACCTG 
CCCACACCCG AAACGAATGC AGAACCGAAC TTTTCGGTGG ACGGCCCACG ATCAATTCCG 
CGTCGTGGCA TTAAATCGCG CCTACTTCAA TTTGGCTCTA GCTATGGATA TATTTTTACG 
ATCGTTAGCT ATTTGATTGG TGTTTTTATC TTCGCCACCA TTGTGGGCCA AGTGGGCAAT 
GTGATAACGA ACCGAAATGC GAATCGCCTG GAGTTCGAGC GCCTTCTGGA TGGGGCCAAG 
ACCTATATGC GGCACCACAA GGTGCCCGGA GGGATGAAGC GGCGCGTGCT GCGATGGTAC 
GACTATAGCT GGTCCCGCGG CAGGATACAG GGTGGCGGTG ACATCAATAC CGCACTGGGC 
CTCCTGCCCG ACAAGCTGAA AACCGAATTG GCCTTACATG TCAACCTGAG CGTTCTGAAG 
AAGGTGACCA TATTCCAAGA GTGCCAGCCC GAGTTCCTTC ACGATCTCGT GCTCAAGATG 
AAGGCCTACA TCTTTACGCC GGGCGACTCC ATTTGCCGTA AGGGCGAGGT GGCCCGCGAG 
ATGTTCATCA TTGCCGATGG CATTCTGGAG GTGCTGAGCG AGACGGGCAA GGTCCTAACC 
ACCATGAAGG CTGGCGATTT TTTTGGCGAA ATCGGCATCC TCAATCTGGA CGGGCTTAAC 
AAACGCACAG CGGATGTGCG CTCCGTGGGC TACTCGGAGC TCTTCTCTCT GTCCCGCGAG 
GATGTCCTGG CGGCCATGAA GGACTACCCC GATGCCCAGG AGATTCTGCA GACCCTGGGC 
CGCAAGCGGC TAATGGAGGT GCGTTGCGTG AACAAAAAGT ACGCCAAGGC GCAGAGTGAC 
AAGGAGGCGG CCGCCTATGC GGCAGCTCAT CCGCACCACC ATCAGAGCCA TCACCAGGGT 
CAGGTGCACC AGAGCGACAG CAGCGAGAAC AGTGCCTCCA AGAAGATCGT GGACAAGCTG 
AAGCACGACG TCAAGGGATT CCGCAACGTG CTGAAGAAGT CCAGGACTTC GCGCAAAAGC 
GATGAATCCT TGGAGATGCA GCCACTGCAC AACACCTCGC CGCGCGGCAG CAAGATCCTG 
CTGAAGCGCA TGTCACGCGT TCGATCGGAC GAAAAGGATG CGGATAGCGC CGAGGCCAAG 
GACGAGTTGC ACGACAAGAC GCCCAGTCCG ATTGGGGCGG GACTGCCATT GCTGCAGCGC 
CTCCGGCTCC TCAAGGAGAA GCAGGAATCG ATCCAGGAGG AGCCCGAACG CGAGTTCAGC 
GAAGGCTTTC CCCTGATCCA GCGACTCCAG CAGTTGAAAA TTAAGAATGA GCCGCAGGCA 
AACGCCGCCG AACCCGGCGT CGTCATGGTC AACACACCGC CCAACATTAG CAGCAAAATT 
GATTCGCATT TCGCTGCCAG CCAGGCAGGT GCATCGCATC CTGGCCCAAA TGGCGGAGCC 
ATCGGCATCT CGGCGCCGGG ACAATCGCTG ACGGTGGCCC AGATCAAGCC CATCATGAAG 
GTGTCCTTCA AGCAGAAGAT CCAGCAGATG CAGGGTGGCG GCGGAGGAGC CAGCAGTTCA 
CCGGGTCCAA GCACGGGAGC CATAGCCAAG AAGGAGCCAC CCAAGTCACT GGCTCTGGTG 
GCCAAGCACG CTACATCCGA GGATCCCGTC GCCGCAGCCG GATTGGGTTT GGGATCTGGT 
TCTAGTTCCG GCATTGCCAC CATATCGAAG AGGGTAGTCA AACCTCAAAG CCATCGGATG 
GCTACGCCGC TGCAATCCGA CACCGACACC GATGGCACGC CCATCAAACC GTGGTCCAAG 
CTGAAACTGG CCACCCTGAT GTCCTCCAGC TACACGAGCT TAACCAACTG TTCGCCGGAT 
GATCTGGCCT CACCGCTGAA GAACTACTCG CTGAGCAACA TACCGCAGCA GATGGAGTCG 
CATACGCGAC CACGTCACCA TAGCGCCAGA AGTAGTTCGA GATCACCGCG TCATGGACAC 
CACCATCACC ATCATCATGG CCACCACCAG CACGGACAGC TTCAGGGGCC AAGCTCCACG 
TGCACCAACG GCTCCACGAC CAGCCAGGTG AACCAGGTGA CGGCGAGTGC AAAGCGTGAT 
TGCTTGCGTC TCCAGAAGCC GGAACAGCAC TTGCCCGACG GCGAACTGAC CGAACTGGGC 
GGCAATGGAC GGAGGAAGCT CTACCAAAGC GTGTTCGATC TGTCGCCGGA ATACTGCGGA 
CTGCCGTTTG TCAAGCGATT GAAGATCCTC AATGAGCGCC AGAAGCTTGC GGAACTGGAG 
CGGGCATTGC AAACCCGTAG CTTTAGCCTC GACTGCTCCA AGTCCGGGCC GGATAGCAAG 
GTGCCCATAA CGGAGTCCTT GTATCGATGT TATAGCGACA CTTCCGGCAT CTACTCGCAG 
TTCCTCAGCA CGTACGAATC AACCACCAGC TCCACCTCGA TATCCACCAA TACTTCCGCC 
TCTGCATCGG CATCCGCCTC CGCATCCGCA TCCGCATCGG ATAGCAACTC CAGGCAATTG 
CAATACGTAC CCCTGCCACT CAGTCCCGAA TCGAATGAGA CCATGGAGCG GCGCAAGCTG 
AAGAGCATAC TCAAGAAACT CCAGATGGGC GGTGGTCAGC AGGAGGAGGC GGGGCCGGGC 
ACGGGAACGG GAGCGGGAGC GGTAATGGGA ACGGGGTCAA AGGTCAACTC CAGTGCCAGC 
CAAAAGGGGC AGGGCCTGCC GGCGGAGCCG ACCTTGGAAG GCCCGCCCGC CAGTGCCAAG 
GAGGAGGAAT CTCCCTTCGG CGGTGCTGCG GCGATCGGTT CGGTGAATAA TGCTTGCGGT 
AACGGTAACG GTAACGGTAG CGTTAGTGGT AACGGTAACG GTAGCGGTAC GGTAAACGAT 
AGCAGTGCTA ATTCGAGTTC GCATCCGAGC AATAACGGCA ACAGTGCGTA TACGTGTCCT 
CCTCCACCGT CAGTGGCTTT TCCGTTTCCG GTACCCATTG CACCAGCATC CGGCTCAGTT 
CCACTACCAT TGGAGCCCGG AGTGTCGAAT GTCTGGTCGC CGACGTTGAC GTTGGTAACG 
CCCACGAATT CGCCGCAGGA GAGCTTCGCG TTTCCTGCCC AACTGCAGGG GGAGTTGGGC 
GGACACGGAA CCGGGCGCGG ACTCGTTGGT GGCGTTGGCA GCGCCTCAGC GTCGATGTTG 
CCAGCGGGAG CGGGAGCAAG TGCATCCGCA TCCGCATCCA CGTCCACGTC CGCCTCGTAT 
GTCGTCGAGT GCTCAGCGTC GTCGAATCAA ATCCTCCATG CCCAGTCCAC CACTGATTTG 
AATTTGAATT TGCAGCTCAG GCAGAACACG ACCAGTTCGA TGGGCGGACC GGGTCCAGCG 
CAACGGATTG AAGGCTTCCC GGAGGCACAG GACTACTTCA ATCAGATCCT GAACGGCATC 
AATCACGTGA TCAAGACGCA CATGAACGAG ATGCACTCCA AGTTCGAGAC GCAATTCTCC 
AGCATGGCTG GTGAGGTGCG ACGACGCGAC GCTATCATTG CCCAGCTGCA GCTCAAGCTG 
CGCTCCATAG AGGGCAAGTC CTCGGCGGCA CCGACATCCT CATCCTCCTC CGCACTGGTT 
CTGCCCATCA GCCGGCGCCA GAAGAAGATG CCCCAGCTGT CGGTGGACGA CGATGAGCCG 
CCGGAGGAGG AGGACAACAG CAGTTCGGGT TCTTCGGCGG AACTTTTGTT TATGCGAGGT 
GACTCCCTGG ACACGGTGTT CACCTCATCG CCGCCCATTC AAGGGGGCAG GCGCAGCACA 
TCGCCGGGTC CTAGTCGTCA TCATGCGGCC TCGGCTAACG CCCTGAACTG TCCGAGCAGC 
TACTACGGAG GACCCGGATC CCTGAGCTCA CGCCACGCCC AGAGCCACCC CAGCTTCTAT 
TCCGGCCAGG GAAATGGACG GAACAGCGGG CGCAGCAGTG GATATCGCGA GTGGAGCGGC 
GCAGCGGAGA GCGGACACCT GGTCAGTGGG AGTGGATCGA GCGGCGCGGT CATGGTGGCC 
GATGTCACGG GCAATCTGGC CCGCCTGTCG GACAGCGTGA TCCTCGACAT TGGCGAGAGT 
TCCAGTTCGA GCTCCTCGTC CAGCAAGATC AACATGGCCG AAGTGGATGA CGACGAGGAG 
GAGGAGGATC CGAGGGTGCG GGATGCTCGA AACGAGGAGG ACATAGACGA TGGCGAAGGT 
GGTTTGGCGA GCCACAACGA CTGGGAGGTG CAGATGCTGG CCGCCGAGAT GGAACGGCAG 
GAACGGAAGC GTGGTCACTC CCTATCCGAC AATCTGGGCG AACTGAAGCA CTGCAGCACG 
TTTCTGCGGC GACGCCGGAA GTTCAGCGAC ACGGAAACGG AATTTAGTGA GACGGACATG 
GAGGAGCAGT TGGCCAGATC GGGCAGCGGC CCGATGGAGC CAAGCGGATC GGCACCATCT 
AGCTCTGGAG CCGGTGGCTC CACATCCGCT GGACATCAGA GCGGCAGCCA GCGACCGAGG 
GCCTCCAGTC TGGATCAGTT CAATCTGCGC TACGGCATCG GGCGCGGAAT CTTTAAAGCA 
ATGAGCATCG ATCGTGATAA AGACAAGCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cngl ( NM_001272642.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259571.2 Drosophila melanogaster cyclic nucleotide-gated ion channel-like, transcript variant I (Cngl), mRNA.

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CloneID	OFa21481
Clone ID Related Accession (Same CDS sequence)	NM_001272642.2
Accession Version	NM_001272642.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cyclic nucleotide-gated ion channel-like, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272642.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAACTGA GCGTGAGCGC GTATCGGGCG CATGCATCCG CACACAAGAA GATCCTCTCC 
AGCGGCTATC ACTCACAGCT CAACTACGGC AGCACTGGAG CGGCAGCGGC ATCCTCCTCG 
TCGTCGGGGG CCACTGCAAC GGGCCTATAT ACGATGGAAG CGCACGAGCA CGAAGCGGGC 
AAGTTTGTCA AGGATGTGGC CCGTCAGGTG CACGACTGCA ACAGCGACAC GGATGTCATC 
AGTCCCACGG GCACCAGTAT CTCCGGTGGC GGAGGAGGCG GCGGAGCAGG CGGTGCCGGC 
GGATCCGGTG GTGCTGGCGG ACACAGCGAC GGTGGCTATC ACAAGCGGCA CCACCACAAG 
ATGGTCCGCG ATGACGACAA CAACAGTGCG GCGCACTCAT CGGACAGCAA GAGTCCCAGC 
CAGCGGAAAT CGCGTATCGG AGATGGAGCT TCAGATCCAG ATTCCGATTG GACACGATCG 
AACCAACGCT GGATGAAGCT ACGCACCACC GTGCAGATCT CATCGGCGAT ACAGAAGAAA 
CCACCGCTCA AGCGCGAAGA CTCCTTCCTG AAGCGTTTCT CGACTAGACA GATACCCGAA 
ACACAGGAAA CTGTTGAAGA TACGGGTTCA GAAAGTGCCT CTGGTGACGT CGACAAGAGT 
GTTAAACGAC GGCGACGCTA TCTGCAGAAA CGACGATCCG TTGTTAATCC AGATGAAAAT 
TTTTACTTCT ATTGGTTAAT GATGTTAACT GTATGTGTTC TATATAATCT ATGGACCCTA 
ATTGTGAGGC AGAGCTTTCC TGAACTGCAG CAATCTGTGC CCACGTTTTG GCTCATCTGC 
GATTCGATGA CAGATGTTGT ATTTATCCTA GATATAATAG TTCAATTACG CACAGGCTAT 
CTGGAGCAGG GCCTAATGGT ATACGACGAC AGGAAGCTGG CCTGTCACTA CGTTCACTCG 
CGCGACTTTA TCTTCGATAT GATAGCGCTG ATACCATTGG ATTTGCTGCA GCTCAAGATG 
GGCACACATC CGCTCTTGCG TTTTACGCGC TTTTTTAAAG TTTATCGATC TGTGAGATTT 
TATTACATCG TAGAAAGTAG AACAGTTTGG CCAAATTTAT GGCGTGTTGT TAACCTAATT 
CATATCCTGT TAATACTGGC ACACTGGTTC GGTTGTTTCT ATTTTTTACT CTCCGAGGCG 
GAGGGCTTTC AGGGCGACTG GGTGTATCCG TATCGACCCG GGGACTATGC CACCCTAACG 
CGAAAGTATC TGGGCAGCCT GTACTGGTCG ACCCTGACAC TGACGACCAT TGGGGACCTG 
CCCACACCCG AAACGAATGC AGAATATATT TTTACGATCG TTAGCTATTT GATTGGTGTT 
TTTATCTTCG CCACCATTGT GGGCCAAGTG GGCAATGTGA TAACGAACCG AAATGCGAAT 
CGCCTGGAGT TCGAGCGCCT TCTGGATGGG GCCAAGACCT ATATGCGGCA CCACAAGGTG 
CCCGGAGGGA TGAAGCGGCG CGTGCTGCGA TGGTACGACT ATAGCTGGTC CCGCGGCAGG 
ATACAGGGTG GCGGTGACAT CAATACCGCA CTGGGCCTCC TGCCCGACAA GCTGAAAACC 
GAATTGGCCT TACATGTCAA CCTGAGCGTT CTGAAGAAGG TGACCATATT CCAAGAGTGC 
CAGCCCGAGT TCCTTCACGA TCTCGTGCTC AAGATGAAGG CCTACATCTT TACGCCGGGC 
GACTCCATTT GCCGTAAGGG CGAGGTGGCC CGCGAGATGT TCATCATTGC CGATGGCATT 
CTGGAGGTGC TGAGCGAGAC GGGCAAGGTC CTAACCACCA TGAAGGCTGG CGATTTTTTT 
GGCGAAATCG GCATCCTCAA TCTGGACGGG CTTAACAAAC GCACAGCGGA TGTGCGCTCC 
GTGGGCTACT CGGAGCTCTT CTCTCTGTCC CGCGAGGATG TCCTGGCGGC CATGAAGGAC 
TACCCCGATG CCCAGGAGAT TCTGCAGACC CTGGGCCGCA AGCGGCTAAT GGAGGTGCGT 
TGCGTGAACA AAAAGTACGC CAAGGCGCAG AGTGACAAGG AGGCGGCCGC CTATGCGGCA 
GCTCATCCGC ACCACCATCA GAGCCATCAC CAGGGTCAGG TGCACCAGAG CGACAGCAGC 
GAGAACAGTG CCTCCAAGAA GATCGTGGAC AAGCTGAAGC ACGACGTCAA GGGATTCCGC 
AACGTGCTGA AGAAGTCCAG GACTTCGCGC AAAAGCGATG AATCCTTGGA GATGCAGCCA 
CTGCACAACA CCTCGCCGCG CGGCAGCAAG ATCCTGCTGA AGCGCATGTC ACGCGTTCGA 
TCGGACGAAA AGGATGCGGA TAGCGCCGAG GCCAAGGACG AGTTGCACGA CAAGACGCCC 
AGTCCGATTG GGGCGGGACT GCCATTGCTG CAGCGCCTCC GGCTCCTCAA GGAGAAGCAG 
GAATCGATCC AGGAGGAGCC CGAACGCGAG TTCAGCGAAG GCTTTCCCCT GATCCAGCGA 
CTCCAGCAGT TGAAAATTAA GAATGAGCCG CAGGCAAACG CCGCCGAACC CGGCGTCGTC 
ATGGTCAACA CACCGCCCAA CATTAGCAGC AAAATTGATT CGCATTTCGC TGCCAGCCAG 
GCAGGTGCAT CGCATCCTGG CCCAAATGGC GGAGCCATCG GCATCTCGGC GCCGGGACAA 
TCGCTGACGG TGGCCCAGAT CAAGCCCATC ATGAAGGTGT CCTTCAAGCA GAAGATCCAG 
CAGATGCAGG GTGGCGGCGG AGGAGCCAGC AGTTCACCGG GTCCAAGCAC GGGAGCCATA 
GCCAAGAAGG AGCCACCCAA GTCACTGGCT CTGGTGGCCA AGCACGCTAC ATCCGAGGAT 
CCCGTCGCCG CAGCCGGATT GGGTTTGGGA TCTGGTTCTA GTTCCGGCAT TGCCACCATA 
TCGAAGAGGG TAGTCAAACC TCAAAGCCAT CGGATGGCTA CGCCGCTGCA ATCCGACACC 
GACACCGATG GCACGCCCAT CAAACCGTGG TCCAAGCTGA AACTGGCCAC CCTGATGTCC 
TCCAGCTACA CGAGCTTAAC CAACTGTTCG CCGGATGATC TGGCCTCACC GCTGAAGAAC 
TACTCGCTGA GCAACATACC GCAGCAGATG GAGTCGCATA CGCGACCACG TCACCATAGC 
GCCAGAAGTA GTTCGAGATC ACCGCGTCAT GGACACCACC ATCACCATCA TCATGGCCAC 
CACCAGCACG GACAGCTTCA GGGGCCAAGC TCCACGTGCA CCAACGGCTC CACGACCAGC 
CAGGTGAACC AGGTGACGGC GAGTGCAAAG CGTGATTGCT TGCGTCTCCA GAAGCCGGAA 
CAGCACTTGC CCGACGGCGA ACTGACCGAA CTGGGCGGCA ATGGACGGAG GAAGCTCTAC 
CAAAGCGTGT TCGATCTGTC GCCGGAATAC TGCGGACTGC CGTTTGTCAA GCGATTGAAG 
ATCCTCAATG AGCGCCAGAA GCTTGCGGAA CTGGAGCGGG CATTGCAAAC CCGTAGCTTT 
AGCCTCGACT GCTCCAAGTC CGGGCCGGAT AGCAAGGTGC CCATAACGGA GTCCTTGTAT 
CGATGTTATA GCGACACTTC CGGCATCTAC TCGCAGTTCC TCAGCACGTA CGAATCAACC 
ACCAGCTCCA CCTCGATATC CACCAATACT TCCGCCTCTG CATCGGCATC CGCCTCCGCA 
TCCGCATCCG CATCGGATAG CAACTCCAGG CAATTGCAAT ACGTACCCCT GCCACTCAGT 
CCCGAATCGA ATGAGACCAT GGAGCGGCGC AAGCTGAAGA GCATACTCAA GAAACTCCAG 
ATGGGCGGTG GTCAGCAGGA GGAGGCGGGG CCGGGCACGG GAACGGGAGC GGGAGCGGTA 
ATGGGAACGG GGTCAAAGGT CAACTCCAGT GCCAGCCAAA AGGGGCAGGG CCTGCCGGCG 
GAGCCGACCT TGGAAGGCCC GCCCGCCAGT GCCAAGGAGG AGGAATCTCC CTTCGGCGGT 
GCTGCGGCGA TCGGTTCGGT GAATAATGCT TGCGGTAACG GTAACGGTAA CGGTAGCGTT 
AGTGGTAACG GTAACGGTAG CGGTACGGTA AACGATAGCA GTGCTAATTC GAGTTCGCAT 
CCGAGCAATA ACGGCAACAG TGCGTATACG TGTCCTCCTC CACCGTCAGT GGCTTTTCCG 
TTTCCGGTAC CCATTGCACC AGCATCCGGC TCAGTTCCAC TACCATTGGA GCCCGGAGTG 
TCGAATGTCT GGTCGCCGAC GTTGACGTTG GTAACGCCCA CGAATTCGCC GCAGGAGAGC 
TTCGCGTTTC CTGCCCAACT GCAGGGGGAG TTGGGCGGAC ACGGAACCGG GCGCGGACTC 
GTTGGTGGCG TTGGCAGCGC CTCAGCGTCG ATGTTGCCAG CGGGAGCGGG AGCAAGTGCA 
TCCGCATCCG CATCCACGTC CACGTCCGCC TCGTATGTCG TCGAGTGCTC AGCGTCGTCG 
AATCAAATCC TCCATGCCCA GTCCACCACT GATTTGAATT TGAATTTGCA GCTCAGGCAG 
AACACGACCA GTTCGATGGG CGGACCGGGT CCAGCGCAAC GGATTGAAGG CTTCCCGGAG 
GCACAGGACT ACTTCAATCA GATCCTGAAC GGCATCAATC ACGTGATCAA GACGCACATG 
AACGAGATGC ACTCCAAGTT CGAGACGCAA TTCTCCAGCA TGGCTGGTGA GGTGCGACGA 
CGCGACGCTA TCATTGCCCA GCTGCAGCTC AAGCTGCGCT CCATAGAGGG CAAGTCCTCG 
GCGGCACCGA CATCCTCATC CTCCTCCGCA CTGGTTCTGC CCATCAGCCG GCGCCAGAAG 
AAGATGCCCC AGCTGTCGGT GGACGACGAT GAGCCGCCGG AGGAGGAGGA CAACAGCAGT 
TCGGGTTCTT CGGCGGAACT TTTGTTTATG CGAGGTGACT CCCTGGACAC GGTGTTCACC 
TCATCGCCGC CCATTCAAGG GGGCAGGCGC AGCACATCGC CGGGTCCTAG TCGTCATCAT 
GCGGCCTCGG CTAACGCCCT GAACTGTCCG AGCAGCTACT ACGGAGGACC CGGATCCCTG 
AGCTCACGCC ACGCCCAGAG CCACCCCAGC TTCTATTCCG GCCAGGGAAA TGGACGGAAC 
AGCGGGCGCA GCAGTGGATA TCGCGAGTGG AGCGGCGCAG CGGAGAGCGG ACACCTGGTC 
AGTGGGAGTG GATCGAGCGG CGCGGTCATG GTGGCCGATG TCACGGGCAA TCTGGCCCGC 
CTGTCGGACA GCGTGATCCT CGACATTGGC GAGAGTTCCA GTTCGAGCTC CTCGTCCAGC 
AAGATCAACA TGGCCGAAGT GGATGACGAC GAGGAGGAGG AGGATCCGAG GGTGCGGGAT 
GCTCGAAACG AGGAGGACAT AGACGATGGC GAAGGTGGTT TGGCGAGCCA CAACGACTGG 
GAGGTGCAGA TGCTGGCCGC CGAGATGGAA CGGCAGGAAC GGAAGCGTGG TCACTCCCTA 
TCCGACAATC TGGGCGAACT GAAGCACTGC AGCACGTTTC TGCGGCGACG CCGGAAGTTC 
AGCGACACGG AAACGGAATT TAGTGAGACG GACATGGAGG AGCAGTTGGC CAGATCGGGC 
AGCGGCCCGA TGGAGCCAAG CGGATCGGCA CCATCTAGCT CTGGAGCCGG TGGCTCCACA 
TCCGCTGGAC ATCAGAGCGG CAGCCAGCGA CCGAGGGCCT CCAGTCTGGA TCAGTTCAAT 
CTGCGCTACG GCATCGGGCG CGGAATCTTT AAAGCAATGA GCATCGATCG TGATAAAGAC 
AAGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259571.2
CDS	1723..7551
Translation	MKLSVSAYRAHASAHKKILSSGYHSQLNYGSTGAAAASSSSSGATATGLYTMEAHEHEAGKFVKDVARQVHDCNSDTDVISPTGTSISGGGGGGGAGGAGGSGGAGGHSDGGYHKRHHHKMVRDDDNNSAAHSSDSKSPSQRKSRIGDGASDPDSDWTRSNQRWMKLRTTVQISSAIQKKPPLKREDSFLKRFSTRQIPETQETVEDTGSESASGDVDKSVKRRRRYLQKRRSVVNPDENFYFYWLMMLTVCVLYNLWTLIVRQSFPELQQSVPTFWLICDSMTDVVFILDIIVQLRTGYLEQGLMVYDDRKLACHYVHSRDFIFDMIALIPLDLLQLKMGTHPLLRFTRFFKVYRSVRFYYIVESRTVWPNLWRVVNLIHILLILAHWFGCFYFLLSEAEGFQGDWVYPYRPGDYATLTRKYLGSLYWSTLTLTTIGDLPTPETNAEYIFTIVSYLIGVFIFATIVGQVGNVITNRNANRLEFERLLDGAKTYMRHHKVPGGMKRRVLRWYDYSWSRGRIQGGGDINTALGLLPDKLKTELALHVNLSVLKKVTIFQECQPEFLHDLVLKMKAYIFTPGDSICRKGEVAREMFIIADGILEVLSETGKVLTTMKAGDFFGEIGILNLDGLNKRTADVRSVGYSELFSLSREDVLAAMKDYPDAQEILQTLGRKRLMEVRCVNKKYAKAQSDKEAAAYAAAHPHHHQSHHQGQVHQSDSSENSASKKIVDKLKHDVKGFRNVLKKSRTSRKSDESLEMQPLHNTSPRGSKILLKRMSRVRSDEKDADSAEAKDELHDKTPSPIGAGLPLLQRLRLLKEKQESIQEEPEREFSEGFPLIQRLQQLKIKNEPQANAAEPGVVMVNTPPNISSKIDSHFAASQAGASHPGPNGGAIGISAPGQSLTVAQIKPIMKVSFKQKIQQMQGGGGGASSSPGPSTGAIAKKEPPKSLALVAKHATSEDPVAAAGLGLGSGSSSGIATISKRVVKPQSHRMATPLQSDTDTDGTPIKPWSKLKLATLMSSSYTSLTNCSPDDLASPLKNYSLSNIPQQMESHTRPRHHSARSSSRSPRHGHHHHHHHGHHQHGQLQGPSSTCTNGSTTSQVNQVTASAKRDCLRLQKPEQHLPDGELTELGGNGRRKLYQSVFDLSPEYCGLPFVKRLKILNERQKLAELERALQTRSFSLDCSKSGPDSKVPITESLYRCYSDTSGIYSQFLSTYESTTSSTSISTNTSASASASASASASASDSNSRQLQYVPLPLSPESNETMERRKLKSILKKLQMGGGQQEEAGPGTGTGAGAVMGTGSKVNSSASQKGQGLPAEPTLEGPPASAKEEESPFGGAAAIGSVNNACGNGNGNGSVSGNGNGSGTVNDSSANSSSHPSNNGNSAYTCPPPPSVAFPFPVPIAPASGSVPLPLEPGVSNVWSPTLTLVTPTNSPQESFAFPAQLQGELGGHGTGRGLVGGVGSASASMLPAGAGASASASASTSTSASYVVECSASSNQILHAQSTTDLNLNLQLRQNTTSSMGGPGPAQRIEGFPEAQDYFNQILNGINHVIKTHMNEMHSKFETQFSSMAGEVRRRDAIIAQLQLKLRSIEGKSSAAPTSSSSSALVLPISRRQKKMPQLSVDDDEPPEEEDNSSSGSSAELLFMRGDSLDTVFTSSPPIQGGRRSTSPGPSRHHAASANALNCPSSYYGGPGSLSSRHAQSHPSFYSGQGNGRNSGRSSGYREWSGAAESGHLVSGSGSSGAVMVADVTGNLARLSDSVILDIGESSSSSSSSSKINMAEVDDDEEEEDPRVRDARNEEDIDDGEGGLASHNDWEVQMLAAEMERQERKRGHSLSDNLGELKHCSTFLRRRRKFSDTETEFSETDMEEQLARSGSGPMEPSGSAPSSSGAGGSTSAGHQSGSQRPRASSLDQFNLRYGIGRGIFKAMSIDRDKDKL

Target ORF information:

RefSeq Version	NM_001272642.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cyclic nucleotide-gated ion channel-like, transcript variant I (Cngl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272642.2

ORF Insert Sequence:

ATGAAACTGA GCGTGAGCGC GTATCGGGCG CATGCATCCG CACACAAGAA GATCCTCTCC 
AGCGGCTATC ACTCACAGCT CAACTACGGC AGCACTGGAG CGGCAGCGGC ATCCTCCTCG 
TCGTCGGGGG CCACTGCAAC GGGCCTATAT ACGATGGAAG CGCACGAGCA CGAAGCGGGC 
AAGTTTGTCA AGGATGTGGC CCGTCAGGTG CACGACTGCA ACAGCGACAC GGATGTCATC 
AGTCCCACGG GCACCAGTAT CTCCGGTGGC GGAGGAGGCG GCGGAGCAGG CGGTGCCGGC 
GGATCCGGTG GTGCTGGCGG ACACAGCGAC GGTGGCTATC ACAAGCGGCA CCACCACAAG 
ATGGTCCGCG ATGACGACAA CAACAGTGCG GCGCACTCAT CGGACAGCAA GAGTCCCAGC 
CAGCGGAAAT CGCGTATCGG AGATGGAGCT TCAGATCCAG ATTCCGATTG GACACGATCG 
AACCAACGCT GGATGAAGCT ACGCACCACC GTGCAGATCT CATCGGCGAT ACAGAAGAAA 
CCACCGCTCA AGCGCGAAGA CTCCTTCCTG AAGCGTTTCT CGACTAGACA GATACCCGAA 
ACACAGGAAA CTGTTGAAGA TACGGGTTCA GAAAGTGCCT CTGGTGACGT CGACAAGAGT 
GTTAAACGAC GGCGACGCTA TCTGCAGAAA CGACGATCCG TTGTTAATCC AGATGAAAAT 
TTTTACTTCT ATTGGTTAAT GATGTTAACT GTATGTGTTC TATATAATCT ATGGACCCTA 
ATTGTGAGGC AGAGCTTTCC TGAACTGCAG CAATCTGTGC CCACGTTTTG GCTCATCTGC 
GATTCGATGA CAGATGTTGT ATTTATCCTA GATATAATAG TTCAATTACG CACAGGCTAT 
CTGGAGCAGG GCCTAATGGT ATACGACGAC AGGAAGCTGG CCTGTCACTA CGTTCACTCG 
CGCGACTTTA TCTTCGATAT GATAGCGCTG ATACCATTGG ATTTGCTGCA GCTCAAGATG 
GGCACACATC CGCTCTTGCG TTTTACGCGC TTTTTTAAAG TTTATCGATC TGTGAGATTT 
TATTACATCG TAGAAAGTAG AACAGTTTGG CCAAATTTAT GGCGTGTTGT TAACCTAATT 
CATATCCTGT TAATACTGGC ACACTGGTTC GGTTGTTTCT ATTTTTTACT CTCCGAGGCG 
GAGGGCTTTC AGGGCGACTG GGTGTATCCG TATCGACCCG GGGACTATGC CACCCTAACG 
CGAAAGTATC TGGGCAGCCT GTACTGGTCG ACCCTGACAC TGACGACCAT TGGGGACCTG 
CCCACACCCG AAACGAATGC AGAATATATT TTTACGATCG TTAGCTATTT GATTGGTGTT 
TTTATCTTCG CCACCATTGT GGGCCAAGTG GGCAATGTGA TAACGAACCG AAATGCGAAT 
CGCCTGGAGT TCGAGCGCCT TCTGGATGGG GCCAAGACCT ATATGCGGCA CCACAAGGTG 
CCCGGAGGGA TGAAGCGGCG CGTGCTGCGA TGGTACGACT ATAGCTGGTC CCGCGGCAGG 
ATACAGGGTG GCGGTGACAT CAATACCGCA CTGGGCCTCC TGCCCGACAA GCTGAAAACC 
GAATTGGCCT TACATGTCAA CCTGAGCGTT CTGAAGAAGG TGACCATATT CCAAGAGTGC 
CAGCCCGAGT TCCTTCACGA TCTCGTGCTC AAGATGAAGG CCTACATCTT TACGCCGGGC 
GACTCCATTT GCCGTAAGGG CGAGGTGGCC CGCGAGATGT TCATCATTGC CGATGGCATT 
CTGGAGGTGC TGAGCGAGAC GGGCAAGGTC CTAACCACCA TGAAGGCTGG CGATTTTTTT 
GGCGAAATCG GCATCCTCAA TCTGGACGGG CTTAACAAAC GCACAGCGGA TGTGCGCTCC 
GTGGGCTACT CGGAGCTCTT CTCTCTGTCC CGCGAGGATG TCCTGGCGGC CATGAAGGAC 
TACCCCGATG CCCAGGAGAT TCTGCAGACC CTGGGCCGCA AGCGGCTAAT GGAGGTGCGT 
TGCGTGAACA AAAAGTACGC CAAGGCGCAG AGTGACAAGG AGGCGGCCGC CTATGCGGCA 
GCTCATCCGC ACCACCATCA GAGCCATCAC CAGGGTCAGG TGCACCAGAG CGACAGCAGC 
GAGAACAGTG CCTCCAAGAA GATCGTGGAC AAGCTGAAGC ACGACGTCAA GGGATTCCGC 
AACGTGCTGA AGAAGTCCAG GACTTCGCGC AAAAGCGATG AATCCTTGGA GATGCAGCCA 
CTGCACAACA CCTCGCCGCG CGGCAGCAAG ATCCTGCTGA AGCGCATGTC ACGCGTTCGA 
TCGGACGAAA AGGATGCGGA TAGCGCCGAG GCCAAGGACG AGTTGCACGA CAAGACGCCC 
AGTCCGATTG GGGCGGGACT GCCATTGCTG CAGCGCCTCC GGCTCCTCAA GGAGAAGCAG 
GAATCGATCC AGGAGGAGCC CGAACGCGAG TTCAGCGAAG GCTTTCCCCT GATCCAGCGA 
CTCCAGCAGT TGAAAATTAA GAATGAGCCG CAGGCAAACG CCGCCGAACC CGGCGTCGTC 
ATGGTCAACA CACCGCCCAA CATTAGCAGC AAAATTGATT CGCATTTCGC TGCCAGCCAG 
GCAGGTGCAT CGCATCCTGG CCCAAATGGC GGAGCCATCG GCATCTCGGC GCCGGGACAA 
TCGCTGACGG TGGCCCAGAT CAAGCCCATC ATGAAGGTGT CCTTCAAGCA GAAGATCCAG 
CAGATGCAGG GTGGCGGCGG AGGAGCCAGC AGTTCACCGG GTCCAAGCAC GGGAGCCATA 
GCCAAGAAGG AGCCACCCAA GTCACTGGCT CTGGTGGCCA AGCACGCTAC ATCCGAGGAT 
CCCGTCGCCG CAGCCGGATT GGGTTTGGGA TCTGGTTCTA GTTCCGGCAT TGCCACCATA 
TCGAAGAGGG TAGTCAAACC TCAAAGCCAT CGGATGGCTA CGCCGCTGCA ATCCGACACC 
GACACCGATG GCACGCCCAT CAAACCGTGG TCCAAGCTGA AACTGGCCAC CCTGATGTCC 
TCCAGCTACA CGAGCTTAAC CAACTGTTCG CCGGATGATC TGGCCTCACC GCTGAAGAAC 
TACTCGCTGA GCAACATACC GCAGCAGATG GAGTCGCATA CGCGACCACG TCACCATAGC 
GCCAGAAGTA GTTCGAGATC ACCGCGTCAT GGACACCACC ATCACCATCA TCATGGCCAC 
CACCAGCACG GACAGCTTCA GGGGCCAAGC TCCACGTGCA CCAACGGCTC CACGACCAGC 
CAGGTGAACC AGGTGACGGC GAGTGCAAAG CGTGATTGCT TGCGTCTCCA GAAGCCGGAA 
CAGCACTTGC CCGACGGCGA ACTGACCGAA CTGGGCGGCA ATGGACGGAG GAAGCTCTAC 
CAAAGCGTGT TCGATCTGTC GCCGGAATAC TGCGGACTGC CGTTTGTCAA GCGATTGAAG 
ATCCTCAATG AGCGCCAGAA GCTTGCGGAA CTGGAGCGGG CATTGCAAAC CCGTAGCTTT 
AGCCTCGACT GCTCCAAGTC CGGGCCGGAT AGCAAGGTGC CCATAACGGA GTCCTTGTAT 
CGATGTTATA GCGACACTTC CGGCATCTAC TCGCAGTTCC TCAGCACGTA CGAATCAACC 
ACCAGCTCCA CCTCGATATC CACCAATACT TCCGCCTCTG CATCGGCATC CGCCTCCGCA 
TCCGCATCCG CATCGGATAG CAACTCCAGG CAATTGCAAT ACGTACCCCT GCCACTCAGT 
CCCGAATCGA ATGAGACCAT GGAGCGGCGC AAGCTGAAGA GCATACTCAA GAAACTCCAG 
ATGGGCGGTG GTCAGCAGGA GGAGGCGGGG CCGGGCACGG GAACGGGAGC GGGAGCGGTA 
ATGGGAACGG GGTCAAAGGT CAACTCCAGT GCCAGCCAAA AGGGGCAGGG CCTGCCGGCG 
GAGCCGACCT TGGAAGGCCC GCCCGCCAGT GCCAAGGAGG AGGAATCTCC CTTCGGCGGT 
GCTGCGGCGA TCGGTTCGGT GAATAATGCT TGCGGTAACG GTAACGGTAA CGGTAGCGTT 
AGTGGTAACG GTAACGGTAG CGGTACGGTA AACGATAGCA GTGCTAATTC GAGTTCGCAT 
CCGAGCAATA ACGGCAACAG TGCGTATACG TGTCCTCCTC CACCGTCAGT GGCTTTTCCG 
TTTCCGGTAC CCATTGCACC AGCATCCGGC TCAGTTCCAC TACCATTGGA GCCCGGAGTG 
TCGAATGTCT GGTCGCCGAC GTTGACGTTG GTAACGCCCA CGAATTCGCC GCAGGAGAGC 
TTCGCGTTTC CTGCCCAACT GCAGGGGGAG TTGGGCGGAC ACGGAACCGG GCGCGGACTC 
GTTGGTGGCG TTGGCAGCGC CTCAGCGTCG ATGTTGCCAG CGGGAGCGGG AGCAAGTGCA 
TCCGCATCCG CATCCACGTC CACGTCCGCC TCGTATGTCG TCGAGTGCTC AGCGTCGTCG 
AATCAAATCC TCCATGCCCA GTCCACCACT GATTTGAATT TGAATTTGCA GCTCAGGCAG 
AACACGACCA GTTCGATGGG CGGACCGGGT CCAGCGCAAC GGATTGAAGG CTTCCCGGAG 
GCACAGGACT ACTTCAATCA GATCCTGAAC GGCATCAATC ACGTGATCAA GACGCACATG 
AACGAGATGC ACTCCAAGTT CGAGACGCAA TTCTCCAGCA TGGCTGGTGA GGTGCGACGA 
CGCGACGCTA TCATTGCCCA GCTGCAGCTC AAGCTGCGCT CCATAGAGGG CAAGTCCTCG 
GCGGCACCGA CATCCTCATC CTCCTCCGCA CTGGTTCTGC CCATCAGCCG GCGCCAGAAG 
AAGATGCCCC AGCTGTCGGT GGACGACGAT GAGCCGCCGG AGGAGGAGGA CAACAGCAGT 
TCGGGTTCTT CGGCGGAACT TTTGTTTATG CGAGGTGACT CCCTGGACAC GGTGTTCACC 
TCATCGCCGC CCATTCAAGG GGGCAGGCGC AGCACATCGC CGGGTCCTAG TCGTCATCAT 
GCGGCCTCGG CTAACGCCCT GAACTGTCCG AGCAGCTACT ACGGAGGACC CGGATCCCTG 
AGCTCACGCC ACGCCCAGAG CCACCCCAGC TTCTATTCCG GCCAGGGAAA TGGACGGAAC 
AGCGGGCGCA GCAGTGGATA TCGCGAGTGG AGCGGCGCAG CGGAGAGCGG ACACCTGGTC 
AGTGGGAGTG GATCGAGCGG CGCGGTCATG GTGGCCGATG TCACGGGCAA TCTGGCCCGC 
CTGTCGGACA GCGTGATCCT CGACATTGGC GAGAGTTCCA GTTCGAGCTC CTCGTCCAGC 
AAGATCAACA TGGCCGAAGT GGATGACGAC GAGGAGGAGG AGGATCCGAG GGTGCGGGAT 
GCTCGAAACG AGGAGGACAT AGACGATGGC GAAGGTGGTT TGGCGAGCCA CAACGACTGG 
GAGGTGCAGA TGCTGGCCGC CGAGATGGAA CGGCAGGAAC GGAAGCGTGG TCACTCCCTA 
TCCGACAATC TGGGCGAACT GAAGCACTGC AGCACGTTTC TGCGGCGACG CCGGAAGTTC 
AGCGACACGG AAACGGAATT TAGTGAGACG GACATGGAGG AGCAGTTGGC CAGATCGGGC 
AGCGGCCCGA TGGAGCCAAG CGGATCGGCA CCATCTAGCT CTGGAGCCGG TGGCTCCACA 
TCCGCTGGAC ATCAGAGCGG CAGCCAGCGA CCGAGGGCCT CCAGTCTGGA TCAGTTCAAT 
CTGCGCTACG GCATCGGGCG CGGAATCTTT AAAGCAATGA GCATCGATCG TGATAAAGAC 
AAGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cngl ( NM_001370020.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001356908.1 Drosophila melanogaster cyclic nucleotide-gated ion channel-like, transcript variant K (Cngl), mRNA.

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CloneID	OFa87676
Clone ID Related Accession (Same CDS sequence)	NM_001370020.1
Accession Version	NM_001370020.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cyclic nucleotide-gated ion channel-like, isoform K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAACTGA GCGTGAGCGC GTATCGGGCG CATGCATCCG CACACAAGAA GATCCTCTCC 
AGCGGCTATC ACTCACAGCT CAACTACGGC AGCACTGGAG CGGCAGCGGC ATCCTCCTCG 
TCGTCGGGGG CCACTGCAAC GGGCCTATAT ACGATGGAAG CGCACGAGCA CGAAGCGGGC 
AAGTTTGTCA AGGATGTGGC CCGTCAGGTG CACGACTGCA ACAGCGACAC GGATGTCATC 
AGTCCCACGG GCACCAGTAT CTCCGGTGGC GGAGGAGGCG GCGGAGCAGG CGGTGCCGGC 
GGATCCGGTG GTGCTGGCGG ACACAGCGAC GGTGGCTATC ACAAGCGGCA CCACCACAAG 
ATGGTCCGCG ATGACGACAA CAACAGTGCG GCGCACTCAT CGGACAGCAA GAGTCCCAGC 
CAGCGGAAAT CGCGTATCGG AGATGGAGCT TCAGATCCAG ATTCCGATTG GACACGATCG 
AACCAACGCT GGATGAAGCT ACGCACCACC GTGCAGATCT CATCGGCGAT ACAGAAGAAA 
CCACCGCTCA AGCGCGAAGA CTCCTTCCTG AAGCGTTTCT CGACTAGACA GATACCCGAA 
ACACAGGAAA CTGTTGAAGA TACGGGTTCA GAAAGTGCCT CTGGTGACGT CGACAAGAGT 
GTTAAACGAC GGCGACGCTA TCTGCAGAAA CGACGATCCG TTGTTAATCC AGATGAAAAT 
TTTTACTTCT ATTGGTTAAT GATGTTAACT GTATGTGTTC TATATAATCT ATGGACCCTA 
ATTGTGAGGC AGAGCTTTCC TGAACTGCAG CAATCTGTGC CCACGTTTTG GCTCATCTGC 
GATTCGATGA CAGATGTTGT ATTTATCCTA GATATAATAG TTCAATTACG CACAGGCTAT 
CTGGAGCAGG GCCTAATGGT ATACGACGAC AGGAAGCTGG CCTGTCACTA CGTTCACTCG 
CGCGACTTTA TCTTCGATAT GATAGCGCTG ATACCATTGG ATTTGCTGCA GCTCAAGATG 
GGCACACATC CGCTCTTGCG TTTTACGCGC TTTTTTAAAG TTTATCGATC TGTGAGATTT 
TATTACATCG TAGAAAGTAG AACAGTTTGG CCAAATTTAT GGCGTGTTGT TAACCTAATT 
CATATCCTGT TAATACTGGC ACACTGGTTC GGTTGTTTCT ATTTTTTACT CTCCGAGGCG 
GAGGGCTTTC AGGGCGACTG GGTGTATCCG TATCGACCCG GGGACTATGC CACCCTAACG 
CGAAAGTATC TGGGCAGCCT GTACTGGTCG ACCCTGACAC TGACGACCAT TGGGGACCTG 
CCCACACCCG AAACGAATGC AGAATATATT TTTACGATCG TTAGCTATTT GATTGGTGTT 
TTTATCTTCG CCACCATTGT GGGCCAAGTG GGCAATGTGA TAACGAACCG AAATGCGAAT 
CGCCTGGAGT TCGAGCGCCT TCTGGATGGG GCCAAGACCT ATATGCGGCA CCACAAGGTG 
CCCGGAGGGA TGAAGCGGCG CGTGCTGCGA TGGTACGACT ATAGCTGGTC CCGCGGCAGG 
ATACAGGGTG GCGGTGACAT CAATACCGCA CTGGGCCTCC TGCCCGACAA GCTGAAAACC 
GAATTGGCCT TACATGTCAA CCTGAGCGTT CTGAAGAAGG TGACCATATT CCAAGAGTGC 
CAGCCCGAGT TCCTTCACGA TCTCGTGCTC AAGATGAAGG CCTACATCTT TACGCCGGGC 
GACTCCATTT GCCGTAAGGG CGAGGTGGCC CGCGAGATGT TCATCATTGC CGATGGCATT 
CTGGAGGTGC TGAGCGAGAC GGGCAAGGTC CTAACCACCA TGAAGGCTGG CGATTTTTTT 
GGCGAAATCG GCATCCTCAA TCTGGACGGG CTTAACAAAC GCACAGCGGA TGTGCGCTCC 
GTGGGCTACT CGGAGCTCTT CTCTCTGTCC CGCGAGGATG TCCTGGCGGC CATGAAGGAC 
TACCCCGATG CCCAGGAGAT TCTGCAGACC CTGGGCCGCA AGCGGCTAAT GGAGGTGCGT 
TGCGTGAACA AAAAGTACGC CAAGGCGCAG AGTGACAAGG AGGCGGCCGC CTATGCGGCA 
GCTCATCCGC ACCACCATCA GAGCCATCAC CAGGGTCAGG TGCACCAGAG CGACAGCAGC 
GAGAACAGTG CCTCCAAGAA GATCGTGGAC AAGCTGAAGC ACGACGTCAA GGGATTCCGC 
AACGTGCTGA AGAAGTCCAG GACTTCGCGC AAAAGCGATG AATCCTTGGA GATGCAGCCA 
CTGCACAACA CCTCGCCGCG CGGCAGCAAG ATCCTGCTGA AGCGCATGTC ACGCGTTCGA 
TCGGACGAAA AGGATGCGGA TAGCGCCGAG GCCAAGGACG AGTTGCACGA CAAGACGCCC 
AGTCCGATTG GGGCGGGACT GCCATTGCTG CAGCGCCTCC GGCTCCTCAA GGAGAAGCAG 
GAATCGATCC AGGAGGAGCC CGAACGCGAG TTCAGCGAAG GCTTTCCCCT GATCCAGCGA 
CTCCAGCAGT TGAAAATTAA GAATGAGCCG CAGGCAAACG CCGCCGAACC CGGCGTCGTC 
ATGGTCAACA CACCGCCCAA CATTAGCAGC AAAATTGATT CGCATTTCGC TGCCAGCCAG 
GCAGGTGCAT CGCATCCTGG CCCAAATGGC GGAGCCATCG GCATCTCGGC GCCGGGACAA 
TCGCTGACGG TGGCCCAGAT CAAGCCCATC ATGAAGGTGT CCTTCAAGCA GAAGATCCAG 
CAGATGCAGG GTGGCGGCGG AGGAGCCAGC AGTTCACCGG GTCCAAGCAC GGGAGCCATA 
GCCAAGAAGG AGCCACCCAA GTCACTGGCT CTGGTGGCCA AGCACGCTAC ATCCGAGGAT 
CCCGTCGCCG CAGCCGGATT GGGTTTGGGA TCTGGTTCTA GTTCCGGCAT TGCCACCATA 
TCGAAGAGGG TAGTCAAACC TCAAAGCCAT CGGATGGCTA CGCCGCTGCA ATCCGACACC 
GACACCGATG GCACGCCCAT CAAACCGTGG TCCAAGCTGA AACTGGCCAC CCTGATGTCC 
TCCAGCTACA CGAGCTTAAC CAACTGTTCG CCGGATGATC TGGCCTCACC GCTGAAGAAC 
TACTCGCTGA GCAACATACC GCAGCAGATG GAGTCGCATA CGCGACCACG TCACCATAGC 
GCCAGAAGTA GTTCGAGATC ACCGCGTCAT GGACACCACC ATCACCATCA TCATGGCCAC 
CACCAGCACG GACAGCTTCA GGGGCCAAGC TCCACGTGCA CCAACGGCTC CACGACCAGC 
CAGGTGAACC AGGTGACGGC GAGTGCAAAG CGTGATTGCT TGCGTCTCCA GAAGCCGGAA 
CAGCACTTGC CCGACGGCGA ACTGACCGAA CTGGGCGGCA ATGGACGGAG GAAGCTCTAC 
CAAAGCGTGT TCGATCTGTC GCCGGAATAC TGCGGACTGC CGTTTGTCAA GCGATTGAAG 
ATCCTCAATG AGCGCCAGAA GCTTGCGGAA CTGGAGCGGG CATTGCAAAC CCGTAGCTTT 
AGCCTCGACT GCTCCAAGTC CGGGCCGGAT AGCAAGGTGC CCATAACGGA GTCCTTGTAT 
CGATGTTATA GCGACACTTC CGGCATCTAC TCGCAGTTCC TCAGCACGTA CGAATCAACC 
ACCAGCTCCA CCTCGATATC CACCAATACT TCCGCCTCTG CATCGGCATC CGCCTCCGCA 
TCCGCATCCG CATCGGATAG CAACTCCAGG CAATTGCAAT ACGTACCCCT GCCACTCAGT 
CCCGAATCGA ATGAGACCAT GGAGCGGCGC AAGCTGAAGA GCATACTCAA GAAACTCCAG 
ATGGGCGGTG GTCAGCAGGA GGAGGCGGGG CCGGGCACGG GAACGGGAGC GGGAGCGGTA 
ATGGGAACGG GGTCAAAGGT CAACTCCAGT GCCAGCCAAA AGGGGCAGGG CCTGCCGGCG 
GAGCCGACCT TGGAAGGCCC GCCCGCCAGT GCCAAGGAGG AGGAATCTCC CTTCGGCGGT 
GCTGCGGCGA TCGGTTCGGT GAATAATGCT TGCGGTAACG GTAACGGTAA CGGTAGCGTT 
AGTGGTAACG GTAACGGTAG CGGTACGGTA AACGATAGCA GTGCTAATTC GAGTTCGCAT 
CCGAGCAATA ACGGCAACAG TGCGTATACG TGTCCTCCTC CACCGTCAGT GGCTTTTCCG 
TTTCCGGTAC CCATTGCACC AGCATCCGGC TCAGTTCCAC TACCATTGGA GCCCGGAGTG 
TCGAATGTCT GGTCGCCGAC GTTGACGTTG GTAACGCCCA CGAATTCGCC GCAGGAGAGC 
TTCGCGTTTC CTGCCCAACT GCAGGGGGAG TTGGGCGGAC ACGGAACCGG GCGCGGACTC 
GTTGGTGGCG TTGGCAGCGC CTCAGCGTCG ATGTTGCCAG CGGGAGCGGG AGCAAGTGCA 
TCCGCATCCG CATCCACGTC CACGTCCGCC TCGTATGTCG TCGAGTGCTC AGCGTCGTCG 
AATCAAATCC TCCATGCCCA GTCCACCACT GATTTGAATT TGAATTTGCA GCTCAGGCAG 
AACACGACCA GTTCGATGGG CGGACCGGGT CCAGCGCAAC GGATTGAAGG CTTCCCGGAG 
GCACAGGACT ACTTCAATCA GATCCTGAAC GGCATCAATC ACGTGATCAA GACGCACATG 
AACGAGATGC ACTCCAAGTT CGAGACGCAA TTCTCCAGCA TGGCTGGTGA GGTGCGACGA 
CGCGACGCTA TCATTGCCCA GCTGCAGCTC AAGCTGCGCT CCATAGAGGG CAAGTCCTCG 
GCGGCACCGA CATCCTCATC CTCCTCCGCA CTGGTTCTGC CCATCAGCCG GCGCCAGAAG 
AAGATGCCCC AGCTGTCGGT GGACGACGAT GAGCCGCCGG AGGAGGAGGA CAACAGCAGT 
TCGGGTTCTT CGGCGGAACT TTTGTTTATG CGAGGTGACT CCCTGGACAC GGTGTTCACC 
TCATCGCCGC CCATTCAAGG GGGCAGGCGC AGCACATCGC CGGGTCCTAG TCGTCATCAT 
GCGGCCTCGG CTAACGCCCT GAACTGTCCG AGCAGCTACT ACGGAGGACC CGGATCCCTG 
AGCTCACGCC ACGCCCAGAG CCACCCCAGC TTCTATTCCG GCCAGGGAAA TGGACGGAAC 
AGCGGGCGCA GCAGTGGATA TCGCGAGTGG AGCGGCGCAG CGGAGAGCGG ACACCTGGTC 
AGTGGGAGTG GATCGAGCGG CGCGGTCATG GTGGCCGATG TCACGGGCAA TCTGGCCCGC 
CTGTCGGACA GCGTGATCCT CGACATTGGC GAGAGTTCCA GTTCGAGCTC CTCGTCCAGC 
AAGATCAACA TGGCCGAAGT GGATGACGAC GAGGAGGAGG AGGATCCGAG GGTGCGGGAT 
GCTCGAAACG AGGAGGACAT AGACGATGGC GAAGGTGGTT TGGCGAGCCA CAACGACTGG 
GAGGTGCAGA TGCTGGCCGC CGAGATGGAA CGGCAGGAAC GGAAGCGTGG TCACTCCCTA 
TCCGACAATC TGGGCGAACT GAAGCACTGC AGCACGTTTC TGCGGCGACG CCGGAAGTTC 
AGCGACACGG AAACGGAATT TAGTGAGACG GACATGGAGG AGCAGTTGGC CAGATCGGGC 
AGCGGCCCGA TGGAGCCAAG CGGATCGGCA CCATCTAGCT CTGGAGCCGG TGGCTCCACA 
TCCGCTGGAC ATCAGAGCGG CAGCCAGCGA CCGAGGGCCT CCAGTCTGGA TCAGTTCAAT 
CTGCGCTACG GCATCGGGCG CGGAATCTTT AAAGCAATGA GCATCGATCG TGATAAAGAC 
AAGCTTTGAG AACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356908.1
CDS	1723..7560
Translation	MKLSVSAYRAHASAHKKILSSGYHSQLNYGSTGAAAASSSSSGATATGLYTMEAHEHEAGKFVKDVARQVHDCNSDTDVISPTGTSISGGGGGGGAGGAGGSGGAGGHSDGGYHKRHHHKMVRDDDNNSAAHSSDSKSPSQRKSRIGDGASDPDSDWTRSNQRWMKLRTTVQISSAIQKKPPLKREDSFLKRFSTRQIPETQETVEDTGSESASGDVDKSVKRRRRYLQKRRSVVNPDENFYFYWLMMLTVCVLYNLWTLIVRQSFPELQQSVPTFWLICDSMTDVVFILDIIVQLRTGYLEQGLMVYDDRKLACHYVHSRDFIFDMIALIPLDLLQLKMGTHPLLRFTRFFKVYRSVRFYYIVESRTVWPNLWRVVNLIHILLILAHWFGCFYFLLSEAEGFQGDWVYPYRPGDYATLTRKYLGSLYWSTLTLTTIGDLPTPETNAEYIFTIVSYLIGVFIFATIVGQVGNVITNRNANRLEFERLLDGAKTYMRHHKVPGGMKRRVLRWYDYSWSRGRIQGGGDINTALGLLPDKLKTELALHVNLSVLKKVTIFQECQPEFLHDLVLKMKAYIFTPGDSICRKGEVAREMFIIADGILEVLSETGKVLTTMKAGDFFGEIGILNLDGLNKRTADVRSVGYSELFSLSREDVLAAMKDYPDAQEILQTLGRKRLMEVRCVNKKYAKAQSDKEAAAYAAAHPHHHQSHHQGQVHQSDSSENSASKKIVDKLKHDVKGFRNVLKKSRTSRKSDESLEMQPLHNTSPRGSKILLKRMSRVRSDEKDADSAEAKDELHDKTPSPIGAGLPLLQRLRLLKEKQESIQEEPEREFSEGFPLIQRLQQLKIKNEPQANAAEPGVVMVNTPPNISSKIDSHFAASQAGASHPGPNGGAIGISAPGQSLTVAQIKPIMKVSFKQKIQQMQGGGGGASSSPGPSTGAIAKKEPPKSLALVAKHATSEDPVAAAGLGLGSGSSSGIATISKRVVKPQSHRMATPLQSDTDTDGTPIKPWSKLKLATLMSSSYTSLTNCSPDDLASPLKNYSLSNIPQQMESHTRPRHHSARSSSRSPRHGHHHHHHHGHHQHGQLQGPSSTCTNGSTTSQVNQVTASAKRDCLRLQKPEQHLPDGELTELGGNGRRKLYQSVFDLSPEYCGLPFVKRLKILNERQKLAELERALQTRSFSLDCSKSGPDSKVPITESLYRCYSDTSGIYSQFLSTYESTTSSTSISTNTSASASASASASASASDSNSRQLQYVPLPLSPESNETMERRKLKSILKKLQMGGGQQEEAGPGTGTGAGAVMGTGSKVNSSASQKGQGLPAEPTLEGPPASAKEEESPFGGAAAIGSVNNACGNGNGNGSVSGNGNGSGTVNDSSANSSSHPSNNGNSAYTCPPPPSVAFPFPVPIAPASGSVPLPLEPGVSNVWSPTLTLVTPTNSPQESFAFPAQLQGELGGHGTGRGLVGGVGSASASMLPAGAGASASASASTSTSASYVVECSASSNQILHAQSTTDLNLNLQLRQNTTSSMGGPGPAQRIEGFPEAQDYFNQILNGINHVIKTHMNEMHSKFETQFSSMAGEVRRRDAIIAQLQLKLRSIEGKSSAAPTSSSSSALVLPISRRQKKMPQLSVDDDEPPEEEDNSSSGSSAELLFMRGDSLDTVFTSSPPIQGGRRSTSPGPSRHHAASANALNCPSSYYGGPGSLSSRHAQSHPSFYSGQGNGRNSGRSSGYREWSGAAESGHLVSGSGSSGAVMVADVTGNLARLSDSVILDIGESSSSSSSSSKINMAEVDDDEEEEDPRVRDARNEEDIDDGEGGLASHNDWEVQMLAAEMERQERKRGHSLSDNLGELKHCSTFLRRRRKFSDTETEFSETDMEEQLARSGSGPMEPSGSAPSSSGAGGSTSAGHQSGSQRPRASSLDQFNLRYGIGRGIFKAMSIDRDKDKL*EL

Target ORF information:

RefSeq Version	NM_001370020.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cyclic nucleotide-gated ion channel-like, transcript variant K (Cngl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370020.1

ORF Insert Sequence:

ATGAAACTGA GCGTGAGCGC GTATCGGGCG CATGCATCCG CACACAAGAA GATCCTCTCC 
AGCGGCTATC ACTCACAGCT CAACTACGGC AGCACTGGAG CGGCAGCGGC ATCCTCCTCG 
TCGTCGGGGG CCACTGCAAC GGGCCTATAT ACGATGGAAG CGCACGAGCA CGAAGCGGGC 
AAGTTTGTCA AGGATGTGGC CCGTCAGGTG CACGACTGCA ACAGCGACAC GGATGTCATC 
AGTCCCACGG GCACCAGTAT CTCCGGTGGC GGAGGAGGCG GCGGAGCAGG CGGTGCCGGC 
GGATCCGGTG GTGCTGGCGG ACACAGCGAC GGTGGCTATC ACAAGCGGCA CCACCACAAG 
ATGGTCCGCG ATGACGACAA CAACAGTGCG GCGCACTCAT CGGACAGCAA GAGTCCCAGC 
CAGCGGAAAT CGCGTATCGG AGATGGAGCT TCAGATCCAG ATTCCGATTG GACACGATCG 
AACCAACGCT GGATGAAGCT ACGCACCACC GTGCAGATCT CATCGGCGAT ACAGAAGAAA 
CCACCGCTCA AGCGCGAAGA CTCCTTCCTG AAGCGTTTCT CGACTAGACA GATACCCGAA 
ACACAGGAAA CTGTTGAAGA TACGGGTTCA GAAAGTGCCT CTGGTGACGT CGACAAGAGT 
GTTAAACGAC GGCGACGCTA TCTGCAGAAA CGACGATCCG TTGTTAATCC AGATGAAAAT 
TTTTACTTCT ATTGGTTAAT GATGTTAACT GTATGTGTTC TATATAATCT ATGGACCCTA 
ATTGTGAGGC AGAGCTTTCC TGAACTGCAG CAATCTGTGC CCACGTTTTG GCTCATCTGC 
GATTCGATGA CAGATGTTGT ATTTATCCTA GATATAATAG TTCAATTACG CACAGGCTAT 
CTGGAGCAGG GCCTAATGGT ATACGACGAC AGGAAGCTGG CCTGTCACTA CGTTCACTCG 
CGCGACTTTA TCTTCGATAT GATAGCGCTG ATACCATTGG ATTTGCTGCA GCTCAAGATG 
GGCACACATC CGCTCTTGCG TTTTACGCGC TTTTTTAAAG TTTATCGATC TGTGAGATTT 
TATTACATCG TAGAAAGTAG AACAGTTTGG CCAAATTTAT GGCGTGTTGT TAACCTAATT 
CATATCCTGT TAATACTGGC ACACTGGTTC GGTTGTTTCT ATTTTTTACT CTCCGAGGCG 
GAGGGCTTTC AGGGCGACTG GGTGTATCCG TATCGACCCG GGGACTATGC CACCCTAACG 
CGAAAGTATC TGGGCAGCCT GTACTGGTCG ACCCTGACAC TGACGACCAT TGGGGACCTG 
CCCACACCCG AAACGAATGC AGAATATATT TTTACGATCG TTAGCTATTT GATTGGTGTT 
TTTATCTTCG CCACCATTGT GGGCCAAGTG GGCAATGTGA TAACGAACCG AAATGCGAAT 
CGCCTGGAGT TCGAGCGCCT TCTGGATGGG GCCAAGACCT ATATGCGGCA CCACAAGGTG 
CCCGGAGGGA TGAAGCGGCG CGTGCTGCGA TGGTACGACT ATAGCTGGTC CCGCGGCAGG 
ATACAGGGTG GCGGTGACAT CAATACCGCA CTGGGCCTCC TGCCCGACAA GCTGAAAACC 
GAATTGGCCT TACATGTCAA CCTGAGCGTT CTGAAGAAGG TGACCATATT CCAAGAGTGC 
CAGCCCGAGT TCCTTCACGA TCTCGTGCTC AAGATGAAGG CCTACATCTT TACGCCGGGC 
GACTCCATTT GCCGTAAGGG CGAGGTGGCC CGCGAGATGT TCATCATTGC CGATGGCATT 
CTGGAGGTGC TGAGCGAGAC GGGCAAGGTC CTAACCACCA TGAAGGCTGG CGATTTTTTT 
GGCGAAATCG GCATCCTCAA TCTGGACGGG CTTAACAAAC GCACAGCGGA TGTGCGCTCC 
GTGGGCTACT CGGAGCTCTT CTCTCTGTCC CGCGAGGATG TCCTGGCGGC CATGAAGGAC 
TACCCCGATG CCCAGGAGAT TCTGCAGACC CTGGGCCGCA AGCGGCTAAT GGAGGTGCGT 
TGCGTGAACA AAAAGTACGC CAAGGCGCAG AGTGACAAGG AGGCGGCCGC CTATGCGGCA 
GCTCATCCGC ACCACCATCA GAGCCATCAC CAGGGTCAGG TGCACCAGAG CGACAGCAGC 
GAGAACAGTG CCTCCAAGAA GATCGTGGAC AAGCTGAAGC ACGACGTCAA GGGATTCCGC 
AACGTGCTGA AGAAGTCCAG GACTTCGCGC AAAAGCGATG AATCCTTGGA GATGCAGCCA 
CTGCACAACA CCTCGCCGCG CGGCAGCAAG ATCCTGCTGA AGCGCATGTC ACGCGTTCGA 
TCGGACGAAA AGGATGCGGA TAGCGCCGAG GCCAAGGACG AGTTGCACGA CAAGACGCCC 
AGTCCGATTG GGGCGGGACT GCCATTGCTG CAGCGCCTCC GGCTCCTCAA GGAGAAGCAG 
GAATCGATCC AGGAGGAGCC CGAACGCGAG TTCAGCGAAG GCTTTCCCCT GATCCAGCGA 
CTCCAGCAGT TGAAAATTAA GAATGAGCCG CAGGCAAACG CCGCCGAACC CGGCGTCGTC 
ATGGTCAACA CACCGCCCAA CATTAGCAGC AAAATTGATT CGCATTTCGC TGCCAGCCAG 
GCAGGTGCAT CGCATCCTGG CCCAAATGGC GGAGCCATCG GCATCTCGGC GCCGGGACAA 
TCGCTGACGG TGGCCCAGAT CAAGCCCATC ATGAAGGTGT CCTTCAAGCA GAAGATCCAG 
CAGATGCAGG GTGGCGGCGG AGGAGCCAGC AGTTCACCGG GTCCAAGCAC GGGAGCCATA 
GCCAAGAAGG AGCCACCCAA GTCACTGGCT CTGGTGGCCA AGCACGCTAC ATCCGAGGAT 
CCCGTCGCCG CAGCCGGATT GGGTTTGGGA TCTGGTTCTA GTTCCGGCAT TGCCACCATA 
TCGAAGAGGG TAGTCAAACC TCAAAGCCAT CGGATGGCTA CGCCGCTGCA ATCCGACACC 
GACACCGATG GCACGCCCAT CAAACCGTGG TCCAAGCTGA AACTGGCCAC CCTGATGTCC 
TCCAGCTACA CGAGCTTAAC CAACTGTTCG CCGGATGATC TGGCCTCACC GCTGAAGAAC 
TACTCGCTGA GCAACATACC GCAGCAGATG GAGTCGCATA CGCGACCACG TCACCATAGC 
GCCAGAAGTA GTTCGAGATC ACCGCGTCAT GGACACCACC ATCACCATCA TCATGGCCAC 
CACCAGCACG GACAGCTTCA GGGGCCAAGC TCCACGTGCA CCAACGGCTC CACGACCAGC 
CAGGTGAACC AGGTGACGGC GAGTGCAAAG CGTGATTGCT TGCGTCTCCA GAAGCCGGAA 
CAGCACTTGC CCGACGGCGA ACTGACCGAA CTGGGCGGCA ATGGACGGAG GAAGCTCTAC 
CAAAGCGTGT TCGATCTGTC GCCGGAATAC TGCGGACTGC CGTTTGTCAA GCGATTGAAG 
ATCCTCAATG AGCGCCAGAA GCTTGCGGAA CTGGAGCGGG CATTGCAAAC CCGTAGCTTT 
AGCCTCGACT GCTCCAAGTC CGGGCCGGAT AGCAAGGTGC CCATAACGGA GTCCTTGTAT 
CGATGTTATA GCGACACTTC CGGCATCTAC TCGCAGTTCC TCAGCACGTA CGAATCAACC 
ACCAGCTCCA CCTCGATATC CACCAATACT TCCGCCTCTG CATCGGCATC CGCCTCCGCA 
TCCGCATCCG CATCGGATAG CAACTCCAGG CAATTGCAAT ACGTACCCCT GCCACTCAGT 
CCCGAATCGA ATGAGACCAT GGAGCGGCGC AAGCTGAAGA GCATACTCAA GAAACTCCAG 
ATGGGCGGTG GTCAGCAGGA GGAGGCGGGG CCGGGCACGG GAACGGGAGC GGGAGCGGTA 
ATGGGAACGG GGTCAAAGGT CAACTCCAGT GCCAGCCAAA AGGGGCAGGG CCTGCCGGCG 
GAGCCGACCT TGGAAGGCCC GCCCGCCAGT GCCAAGGAGG AGGAATCTCC CTTCGGCGGT 
GCTGCGGCGA TCGGTTCGGT GAATAATGCT TGCGGTAACG GTAACGGTAA CGGTAGCGTT 
AGTGGTAACG GTAACGGTAG CGGTACGGTA AACGATAGCA GTGCTAATTC GAGTTCGCAT 
CCGAGCAATA ACGGCAACAG TGCGTATACG TGTCCTCCTC CACCGTCAGT GGCTTTTCCG 
TTTCCGGTAC CCATTGCACC AGCATCCGGC TCAGTTCCAC TACCATTGGA GCCCGGAGTG 
TCGAATGTCT GGTCGCCGAC GTTGACGTTG GTAACGCCCA CGAATTCGCC GCAGGAGAGC 
TTCGCGTTTC CTGCCCAACT GCAGGGGGAG TTGGGCGGAC ACGGAACCGG GCGCGGACTC 
GTTGGTGGCG TTGGCAGCGC CTCAGCGTCG ATGTTGCCAG CGGGAGCGGG AGCAAGTGCA 
TCCGCATCCG CATCCACGTC CACGTCCGCC TCGTATGTCG TCGAGTGCTC AGCGTCGTCG 
AATCAAATCC TCCATGCCCA GTCCACCACT GATTTGAATT TGAATTTGCA GCTCAGGCAG 
AACACGACCA GTTCGATGGG CGGACCGGGT CCAGCGCAAC GGATTGAAGG CTTCCCGGAG 
GCACAGGACT ACTTCAATCA GATCCTGAAC GGCATCAATC ACGTGATCAA GACGCACATG 
AACGAGATGC ACTCCAAGTT CGAGACGCAA TTCTCCAGCA TGGCTGGTGA GGTGCGACGA 
CGCGACGCTA TCATTGCCCA GCTGCAGCTC AAGCTGCGCT CCATAGAGGG CAAGTCCTCG 
GCGGCACCGA CATCCTCATC CTCCTCCGCA CTGGTTCTGC CCATCAGCCG GCGCCAGAAG 
AAGATGCCCC AGCTGTCGGT GGACGACGAT GAGCCGCCGG AGGAGGAGGA CAACAGCAGT 
TCGGGTTCTT CGGCGGAACT TTTGTTTATG CGAGGTGACT CCCTGGACAC GGTGTTCACC 
TCATCGCCGC CCATTCAAGG GGGCAGGCGC AGCACATCGC CGGGTCCTAG TCGTCATCAT 
GCGGCCTCGG CTAACGCCCT GAACTGTCCG AGCAGCTACT ACGGAGGACC CGGATCCCTG 
AGCTCACGCC ACGCCCAGAG CCACCCCAGC TTCTATTCCG GCCAGGGAAA TGGACGGAAC 
AGCGGGCGCA GCAGTGGATA TCGCGAGTGG AGCGGCGCAG CGGAGAGCGG ACACCTGGTC 
AGTGGGAGTG GATCGAGCGG CGCGGTCATG GTGGCCGATG TCACGGGCAA TCTGGCCCGC 
CTGTCGGACA GCGTGATCCT CGACATTGGC GAGAGTTCCA GTTCGAGCTC CTCGTCCAGC 
AAGATCAACA TGGCCGAAGT GGATGACGAC GAGGAGGAGG AGGATCCGAG GGTGCGGGAT 
GCTCGAAACG AGGAGGACAT AGACGATGGC GAAGGTGGTT TGGCGAGCCA CAACGACTGG 
GAGGTGCAGA TGCTGGCCGC CGAGATGGAA CGGCAGGAAC GGAAGCGTGG TCACTCCCTA 
TCCGACAATC TGGGCGAACT GAAGCACTGC AGCACGTTTC TGCGGCGACG CCGGAAGTTC 
AGCGACACGG AAACGGAATT TAGTGAGACG GACATGGAGG AGCAGTTGGC CAGATCGGGC 
AGCGGCCCGA TGGAGCCAAG CGGATCGGCA CCATCTAGCT CTGGAGCCGG TGGCTCCACA 
TCCGCTGGAC ATCAGAGCGG CAGCCAGCGA CCGAGGGCCT CCAGTCTGGA TCAGTTCAAT 
CTGCGCTACG GCATCGGGCG CGGAATCTTT AAAGCAATGA GCATCGATCG TGATAAAGAC 
AAGCTTTGAG AACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cngl ( NM_001258761.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245690.1 Drosophila melanogaster cyclic nucleotide-gated ion channel-like, transcript variant D (Cngl), mRNA.

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CloneID	OFa02727
Clone ID Related Accession (Same CDS sequence)	NM_001258761.3
Accession Version	NM_001258761.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cyclic nucleotide-gated ion channel-like, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258761.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAACTGA GCGTGAGCGC GTATCGGGCG CATGCATCCG CACACAAGAA GATCCTCTCC 
AGCGGCTATC ACTCACAGCT CAACTACGGC AGCACTGGAG CGGCAGCGGC ATCCTCCTCG 
TCGTCGGGGG CCACTGCAAC GGGCCTATAT ACGATGGAAG CGCACGAGCA CGAAGCGGGC 
AAGTTTGTCA AGGATGTGGC CCGTCAGGTG CACGACTGCA ACAGCGACAC GGATGTCATC 
AGTCCCACGG GCACCAGTAT CTCCGGTGGC GGAGGAGGCG GCGGAGCAGG CGGTGCCGGC 
GGATCCGGTG GTGCTGGCGG ACACAGCGAC GGTGGCTATC ACAAGCGGCA CCACCACAAG 
ATGGTCCGCG ATGACGACAA CAACAGTGCG GCGCACTCAT CGGACAGCAA GAGTCCCAGC 
CAGCGGACAC GATCGAACCA ACGCTGGATG AAGCTACGCA CCACCGTGCA GATCTCATCG 
GCGATACAGA AGAAACCACC GCTCAAGCGC GAAGACTCCT TCCTGAAGCG TTTCTCGACT 
AGACAGATAC CCGAAACACA GGAAACTGTT GAAGATACGG GTTCAGAAAG TGCCTCTGGT 
GACGTCGACA AGAGTGTTAA ACGACGGCGA CGCTATCTGC AGAAACGACG ATCCGTTGTT 
AATCCAGATG AAAATTTTTA CTTCTATTGG TTAATGATGT TAACTGTATG TGTTCTATAT 
AATCTATGGA CCCTAATTGT GAGGCAGAGC TTTCCTGAAC TGCAGCAATC TGTGCCCACG 
TTTTGGCTCA TCTGCGATTC GATGACAGAT GTTGTATTTA TCCTAGATAT AATAGTTCAA 
TTACGCACAG GCTATCTGGA GCAGGGCCTA ATGGTATACG ACGACAGGAA GCTGGCCTGT 
CACTACGTTC ACTCGCGCGA CTTTATCTTC GATATGATAG CGCTGATACC ATTGGATTTG 
CTGCAGCTCA AGATGGGCAC ACATCCGCTC TTGCGTTTTA CGCGCTTTTT TAAAGTTTAT 
CGATCTGTGA GATTTTATTA CATCGTAGAA AGTAGAACAG TTTGGCCAAA TTTATGGCGT 
GTTGTTAACC TAATTCATAT CCTGTTAATA CTGGCACACT GGTTCGGTTG TTTCTATTTT 
TTACTCTCCG AGGCGGAGGG CTTTCAGGGC GACTGGGTGT ATCCGTATCG ACCCGGGGAC 
TATGCCACCC TAACGCGAAA GTATCTGGGC AGCCTGTACT GGTCGACCCT GACACTGACG 
ACCATTGGGG ACCTGCCCAC ACCCGAAACG AATGCAGAAT ATATTTTTAC GATCGTTAGC 
TATTTGATTG GTGTTTTTAT CTTCGCCACC ATTGTGGGCC AAGTGGGCAA TGTGATAACG 
AACCGAAATG CGAATCGCCT GGAGTTCGAG CGCCTTCTGG ATGGGGCCAA GACCTATATG 
CGGCACCACA AGGTGCCCGG AGGGATGAAG CGGCGCGTGC TGCGATGGTA CGACTATAGC 
TGGTCCCGCG GCAGGATACA GGGTGGCGGT GACATCAATA CCGCACTGGG CCTCCTGCCC 
GACAAGCTGA AAACCGAATT GGCCTTACAT GTCAACCTGA GCGTTCTGAA GAAGGTGACC 
ATATTCCAAG AGTGCCAGCC CGAGTTCCTT CACGATCTCG TGCTCAAGAT GAAGGCCTAC 
ATCTTTACGC CGGGCGACTC CATTTGCCGT AAGGGCGAGG TGGCCCGCGA GATGTTCATC 
ATTGCCGATG GCATTCTGGA GGTGCTGAGC GAGACGGGCA AGGTCCTAAC CACCATGAAG 
GCTGGCGATT TTTTTGGCGA AATCGGCATC CTCAATCTGG ACGGGCTTAA CAAACGCACA 
GCGGATGTGC GCTCCGTGGG CTACTCGGAG CTCTTCTCTC TGTCCCGCGA GGATGTCCTG 
GCGGCCATGA AGGACTACCC CGATGCCCAG GAGATTCTGC AGACCCTGGG CCGCAAGCGG 
CTAATGGAGG TGCGTTGCGT GAACAAAAAG TACGCCAAGG CGCAGAGTGA CAAGGAGGCG 
GCCGCCTATG CGGCAGCTCA TCCGCACCAC CATCAGAGCC ATCACCAGGG TCAGGTGCAC 
CAGAGCGACA GCAGCGAGAA CAGTGCCTCC AAGAAGATCG TGGACAAGCT GAAGCACGAC 
GTCAAGGGAT TCCGCAACGT GCTGAAGAAG TCCAGGACTT CGCGCAAAAG CGATGAATCC 
TTGGAGATGC AGCCACTGCA CAACACCTCG CCGCGCGGCA GCAAGATCCT GCTGAAGCGC 
ATGTCACGCG TTCGATCGGA CGAAAAGGAT GCGGATAGCG CCGAGGCCAA GGACGAGTTG 
CACGACAAGA CGCCCAGTCC GATTGGGGCG GGACTGCCAT TGCTGCAGCG CCTCCGGCTC 
CTCAAGGAGA AGCAGGATCG CGAAGAGCGA GCTGTTAAGT CCACACCACC ACAGAAATCT 
CCACCTCACT CACATGTAAC GTGTACGTTA TCGCCGCAGG AATCGATCCA GGAGGAGCCC 
GAACGCGAGT TCAGCGAAGG CTTTCCCCTG ATCCAGCGAC TCCAGCAGTT GAAAATTAAG 
AATGAGCCGC AGGCAAACGC CGCCGAACCC GGCGTCGTCA TGGTCAACAC ACCGCCCAAC 
ATTAGCAGCA AAATTGATTC GCATTTCGCT GCCAGCCAGG CAGGTGCATC GCATCCTGGC 
CCAAATGGCG GAGCCATCGG CATCTCGGCG CCGGGACAAT CGCTGACGGT GGCCCAGATC 
AAGCCCATCA TGAAGGTGTC CTTCAAGCAG AAGATCCAGC AGATGCAGGG TGGCGGCGGA 
GGAGCCAGCA GTTCACCGGG TCCAAGCACG GGAGCCATAG CCAAGAAGGA GCCACCCAAG 
TCACTGGCTC TGGTGGCCAA GCACGCTACA TCCGAGGATC CCGTCGCCGC AGCCGGATTG 
GGTTTGGGAT CTGGTTCTAG TTCCGGCATT GCCACCATAT CGAAGAGGGT AGTCAAACCT 
CAAAGCCATC GGATGGCTAC GCCGCTGCAA TCCGACACCG ACACCGATGG CACGCCCATC 
AAACCGTGGT CCAAGCTGAA ACTGGCCACC CTGATGTCCT CCAGCTACAC GAGCTTAACC 
AACTGTTCGC CGGATGATCT GGCCTCACCG CTGAAGAACT ACTCGCTGAG CAACATACCG 
CAGCAGATGG AGTCGCATAC GCGACCACGT CACCATAGCG CCAGAAGTAG TTCGAGATCA 
CCGCGTCATG GACACCACCA TCACCATCAT CATGGCCACC ACCAGCACGG ACAGCTTCAG 
GGGCCAAGCT CCACGTGCAC CAACGGCTCC ACGACCAGCC AGGTGAACCA GGTGACGGCG 
AGTGCAAAGC GTGATTGCTT GCGTCTCCAG AAGCCGGAAC AGCACTTGCC CGACGGCGAA 
CTGACCGAAC TGGGCGGCAA TGGACGGAGG AAGCTCTACC AAAGCGTGTT CGATCTGTCG 
CCGGAATACT GCGGACTGCC GTTTGTCAAG CGATTGAAGA TCCTCAATGA GCGCCAGAAG 
CTTGCGGAAC TGGAGCGGGC ATTGCAAACC CGTAGCTTTA GCCTCGACTG CTCCAAGTCC 
GGGCCGGATA GCAAGGTGCC CATAACGGAG TCCTTGTATC GATGTTATAG CGACACTTCC 
GGCATCTACT CGCAGTTCCT CAGCACGTAC GAATCAACCA CCAGCTCCAC CTCGATATCC 
ACCAATACTT CCGCCTCTGC ATCGGCATCC GCCTCCGCAT CCGCATCCGC ATCGGATAGC 
AACTCCAGGC AATTGCAATA CGTACCCCTG CCACTCAGTC CCGAATCGAA TGAGACCATG 
GAGCGGCGCA AGCTGAAGAG CATACTCAAG AAACTCCAGA TGGGCGGTGG TCAGCAGGAG 
GAGGCGGGGC CGGGCACGGG AACGGGAGCG GGAGCGGTAA TGGGAACGGG GTCAAAGGTC 
AACTCCAGTG CCAGCCAAAA GGGGCAGGGC CTGCCGGCGG AGCCGACCTT GGAAGGCCCG 
CCCGCCAGTG CCAAGGAGGA GGAATCTCCC TTCGGCGGTG CTGCGGCGAT CGGTTCGGTG 
AATAATGCTT GCGGTAACGG TAACGGTAAC GGTAGCGTTA GTGGTAACGG TAACGGTAGC 
GGTACGGTAA ACGATAGCAG TGCTAATTCG AGTTCGCATC CGAGCAATAA CGGCAACAGT 
GCGTATACGT GTCCTCCTCC ACCGTCAGTG GCTTTTCCGT TTCCGGTACC CATTGCACCA 
GCATCCGGCT CAGTTCCACT ACCATTGGAG CCCGGAGTGT CGAATGTCTG GTCGCCGACG 
TTGACGTTGG TAACGCCCAC GAATTCGCCG CAGGAGAGCT TCGCGTTTCC TGCCCAACTG 
CAGGGGGAGT TGGGCGGACA CGGAACCGGG CGCGGACTCG TTGGTGGCGT TGGCAGCGCC 
TCAGCGTCGA TGTTGCCAGC GGGAGCGGGA GCAAGTGCAT CCGCATCCGC ATCCACGTCC 
ACGTCCGCCT CGTATGTCGT CGAGTGCTCA GCGTCGTCGA ATCAAATCCT CCATGCCCAG 
TCCACCACTG ATTTGAATTT GAATTTGCAG CTCAGGCAGA ACACGACCAG TTCGATGGGC 
GGACCGGGTC CAGCGCAACG GATTGAAGGC TTCCCGGAGG CACAGGACTA CTTCAATCAG 
ATCCTGAACG GCATCAATCA CGTGATCAAG ACGCACATGA ACGAGATGCA CTCCAAGTTC 
GAGACGCAAT TCTCCAGCAT GGCTGGTGAG GTGCGACGAC GCGACGCTAT CATTGCCCAG 
CTGCAGCTCA AGCTGCGCTC CATAGAGGGC AAGTCCTCGG CGGCACCGAC ATCCTCATCC 
TCCTCCGCAC TGGTTCTGCC CATCAGCCGG CGCCAGAAGA AGATGCCCCA GCTGTCGGTG 
GACGACGATG AGCCGCCGGA GGAGGAGGAC AACAGCAGTT CGGGTTCTTC GGCGGAACTT 
TTGTTTATGC GAGGTGACTC CCTGGACACG GTGTTCACCT CATCGCCGCC CATTCAAGGG 
GGCAGGCGCA GCACATCGCC GGGTCCTAGT CGTCATCATG CGGCCTCGGC TAACGCCCTG 
AACTGTCCGA GCAGCTACTA CGGAGGACCC GGATCCCTGA GCTCACGCCA CGCCCAGAGC 
CACCCCAGCT TCTATTCCGG CCAGGGAAAT GGACGGAACA GCGGGCGCAG CAGTGGATAT 
CGCGAGTGGA GCGGCGCAGC GGAGAGCGGA CACCTGGTCA GTGGGAGTGG ATCGAGCGGC 
GCGGTCATGG TGGCCGATGT CACGGGCAAT CTGGCCCGCC TGTCGGACAG CGTGATCCTC 
GACATTGGCG AGAGTTCCAG TTCGAGCTCC TCGTCCAGCA AGATCAACAT GGCCGAAGTG 
GATGACGACG AGGAGGAGGA GGATCCGAGG GTGCGGGATG CTCGAAACGA GGAGGACATA 
GACGATGGCG AAGGTGGTTT GGCGAGCCAC AACGACTGGG AGGTGCAGAT GCTGGCCGCC 
GAGATGGAAC GGCAGGAACG GAAGCGTGGT CACTCCCTAT CCGACAATCT GGGCGAACTG 
AAGCACTGCA GCACGTTTCT GCGGCGACGC CGGAAGTTCA GCGACACGGA AACGGAATTT 
AGTGAGACGG ACATGGAGGA GCAGTTGGCC AGATCGGGCA GCGGCCCGAT GGAGCCAAGC 
GGATCGGCAC CATCTAGCTC TGGAGCCGGT GGCTCCACAT CCGCTGGACA TCAGAGCGGC 
AGCCAGCGAC CGAGGGCCTC CAGTCTGGAT CAGTTCAATC TGCGCTACGG CATCGGGCGC 
GGAATCTTTA AAGCAATGAG CATCGATCGT GATAAAGACA AGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245690.1
CDS	2250..8117
Translation	MKLSVSAYRAHASAHKKILSSGYHSQLNYGSTGAAAASSSSSGATATGLYTMEAHEHEAGKFVKDVARQVHDCNSDTDVISPTGTSISGGGGGGGAGGAGGSGGAGGHSDGGYHKRHHHKMVRDDDNNSAAHSSDSKSPSQRTRSNQRWMKLRTTVQISSAIQKKPPLKREDSFLKRFSTRQIPETQETVEDTGSESASGDVDKSVKRRRRYLQKRRSVVNPDENFYFYWLMMLTVCVLYNLWTLIVRQSFPELQQSVPTFWLICDSMTDVVFILDIIVQLRTGYLEQGLMVYDDRKLACHYVHSRDFIFDMIALIPLDLLQLKMGTHPLLRFTRFFKVYRSVRFYYIVESRTVWPNLWRVVNLIHILLILAHWFGCFYFLLSEAEGFQGDWVYPYRPGDYATLTRKYLGSLYWSTLTLTTIGDLPTPETNAEYIFTIVSYLIGVFIFATIVGQVGNVITNRNANRLEFERLLDGAKTYMRHHKVPGGMKRRVLRWYDYSWSRGRIQGGGDINTALGLLPDKLKTELALHVNLSVLKKVTIFQECQPEFLHDLVLKMKAYIFTPGDSICRKGEVAREMFIIADGILEVLSETGKVLTTMKAGDFFGEIGILNLDGLNKRTADVRSVGYSELFSLSREDVLAAMKDYPDAQEILQTLGRKRLMEVRCVNKKYAKAQSDKEAAAYAAAHPHHHQSHHQGQVHQSDSSENSASKKIVDKLKHDVKGFRNVLKKSRTSRKSDESLEMQPLHNTSPRGSKILLKRMSRVRSDEKDADSAEAKDELHDKTPSPIGAGLPLLQRLRLLKEKQDREERAVKSTPPQKSPPHSHVTCTLSPQESIQEEPEREFSEGFPLIQRLQQLKIKNEPQANAAEPGVVMVNTPPNISSKIDSHFAASQAGASHPGPNGGAIGISAPGQSLTVAQIKPIMKVSFKQKIQQMQGGGGGASSSPGPSTGAIAKKEPPKSLALVAKHATSEDPVAAAGLGLGSGSSSGIATISKRVVKPQSHRMATPLQSDTDTDGTPIKPWSKLKLATLMSSSYTSLTNCSPDDLASPLKNYSLSNIPQQMESHTRPRHHSARSSSRSPRHGHHHHHHHGHHQHGQLQGPSSTCTNGSTTSQVNQVTASAKRDCLRLQKPEQHLPDGELTELGGNGRRKLYQSVFDLSPEYCGLPFVKRLKILNERQKLAELERALQTRSFSLDCSKSGPDSKVPITESLYRCYSDTSGIYSQFLSTYESTTSSTSISTNTSASASASASASASASDSNSRQLQYVPLPLSPESNETMERRKLKSILKKLQMGGGQQEEAGPGTGTGAGAVMGTGSKVNSSASQKGQGLPAEPTLEGPPASAKEEESPFGGAAAIGSVNNACGNGNGNGSVSGNGNGSGTVNDSSANSSSHPSNNGNSAYTCPPPPSVAFPFPVPIAPASGSVPLPLEPGVSNVWSPTLTLVTPTNSPQESFAFPAQLQGELGGHGTGRGLVGGVGSASASMLPAGAGASASASASTSTSASYVVECSASSNQILHAQSTTDLNLNLQLRQNTTSSMGGPGPAQRIEGFPEAQDYFNQILNGINHVIKTHMNEMHSKFETQFSSMAGEVRRRDAIIAQLQLKLRSIEGKSSAAPTSSSSSALVLPISRRQKKMPQLSVDDDEPPEEEDNSSSGSSAELLFMRGDSLDTVFTSSPPIQGGRRSTSPGPSRHHAASANALNCPSSYYGGPGSLSSRHAQSHPSFYSGQGNGRNSGRSSGYREWSGAAESGHLVSGSGSSGAVMVADVTGNLARLSDSVILDIGESSSSSSSSSKINMAEVDDDEEEEDPRVRDARNEEDIDDGEGGLASHNDWEVQMLAAEMERQERKRGHSLSDNLGELKHCSTFLRRRRKFSDTETEFSETDMEEQLARSGSGPMEPSGSAPSSSGAGGSTSAGHQSGSQRPRASSLDQFNLRYGIGRGIFKAMSIDRDKDKL

Target ORF information:

RefSeq Version	NM_001258761.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cyclic nucleotide-gated ion channel-like, transcript variant D (Cngl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258761.3

ORF Insert Sequence:

ATGAAACTGA GCGTGAGCGC GTATCGGGCG CATGCATCCG CACACAAGAA GATCCTCTCC 
AGCGGCTATC ACTCACAGCT CAACTACGGC AGCACTGGAG CGGCAGCGGC ATCCTCCTCG 
TCGTCGGGGG CCACTGCAAC GGGCCTATAT ACGATGGAAG CGCACGAGCA CGAAGCGGGC 
AAGTTTGTCA AGGATGTGGC CCGTCAGGTG CACGACTGCA ACAGCGACAC GGATGTCATC 
AGTCCCACGG GCACCAGTAT CTCCGGTGGC GGAGGAGGCG GCGGAGCAGG CGGTGCCGGC 
GGATCCGGTG GTGCTGGCGG ACACAGCGAC GGTGGCTATC ACAAGCGGCA CCACCACAAG 
ATGGTCCGCG ATGACGACAA CAACAGTGCG GCGCACTCAT CGGACAGCAA GAGTCCCAGC 
CAGCGGACAC GATCGAACCA ACGCTGGATG AAGCTACGCA CCACCGTGCA GATCTCATCG 
GCGATACAGA AGAAACCACC GCTCAAGCGC GAAGACTCCT TCCTGAAGCG TTTCTCGACT 
AGACAGATAC CCGAAACACA GGAAACTGTT GAAGATACGG GTTCAGAAAG TGCCTCTGGT 
GACGTCGACA AGAGTGTTAA ACGACGGCGA CGCTATCTGC AGAAACGACG ATCCGTTGTT 
AATCCAGATG AAAATTTTTA CTTCTATTGG TTAATGATGT TAACTGTATG TGTTCTATAT 
AATCTATGGA CCCTAATTGT GAGGCAGAGC TTTCCTGAAC TGCAGCAATC TGTGCCCACG 
TTTTGGCTCA TCTGCGATTC GATGACAGAT GTTGTATTTA TCCTAGATAT AATAGTTCAA 
TTACGCACAG GCTATCTGGA GCAGGGCCTA ATGGTATACG ACGACAGGAA GCTGGCCTGT 
CACTACGTTC ACTCGCGCGA CTTTATCTTC GATATGATAG CGCTGATACC ATTGGATTTG 
CTGCAGCTCA AGATGGGCAC ACATCCGCTC TTGCGTTTTA CGCGCTTTTT TAAAGTTTAT 
CGATCTGTGA GATTTTATTA CATCGTAGAA AGTAGAACAG TTTGGCCAAA TTTATGGCGT 
GTTGTTAACC TAATTCATAT CCTGTTAATA CTGGCACACT GGTTCGGTTG TTTCTATTTT 
TTACTCTCCG AGGCGGAGGG CTTTCAGGGC GACTGGGTGT ATCCGTATCG ACCCGGGGAC 
TATGCCACCC TAACGCGAAA GTATCTGGGC AGCCTGTACT GGTCGACCCT GACACTGACG 
ACCATTGGGG ACCTGCCCAC ACCCGAAACG AATGCAGAAT ATATTTTTAC GATCGTTAGC 
TATTTGATTG GTGTTTTTAT CTTCGCCACC ATTGTGGGCC AAGTGGGCAA TGTGATAACG 
AACCGAAATG CGAATCGCCT GGAGTTCGAG CGCCTTCTGG ATGGGGCCAA GACCTATATG 
CGGCACCACA AGGTGCCCGG AGGGATGAAG CGGCGCGTGC TGCGATGGTA CGACTATAGC 
TGGTCCCGCG GCAGGATACA GGGTGGCGGT GACATCAATA CCGCACTGGG CCTCCTGCCC 
GACAAGCTGA AAACCGAATT GGCCTTACAT GTCAACCTGA GCGTTCTGAA GAAGGTGACC 
ATATTCCAAG AGTGCCAGCC CGAGTTCCTT CACGATCTCG TGCTCAAGAT GAAGGCCTAC 
ATCTTTACGC CGGGCGACTC CATTTGCCGT AAGGGCGAGG TGGCCCGCGA GATGTTCATC 
ATTGCCGATG GCATTCTGGA GGTGCTGAGC GAGACGGGCA AGGTCCTAAC CACCATGAAG 
GCTGGCGATT TTTTTGGCGA AATCGGCATC CTCAATCTGG ACGGGCTTAA CAAACGCACA 
GCGGATGTGC GCTCCGTGGG CTACTCGGAG CTCTTCTCTC TGTCCCGCGA GGATGTCCTG 
GCGGCCATGA AGGACTACCC CGATGCCCAG GAGATTCTGC AGACCCTGGG CCGCAAGCGG 
CTAATGGAGG TGCGTTGCGT GAACAAAAAG TACGCCAAGG CGCAGAGTGA CAAGGAGGCG 
GCCGCCTATG CGGCAGCTCA TCCGCACCAC CATCAGAGCC ATCACCAGGG TCAGGTGCAC 
CAGAGCGACA GCAGCGAGAA CAGTGCCTCC AAGAAGATCG TGGACAAGCT GAAGCACGAC 
GTCAAGGGAT TCCGCAACGT GCTGAAGAAG TCCAGGACTT CGCGCAAAAG CGATGAATCC 
TTGGAGATGC AGCCACTGCA CAACACCTCG CCGCGCGGCA GCAAGATCCT GCTGAAGCGC 
ATGTCACGCG TTCGATCGGA CGAAAAGGAT GCGGATAGCG CCGAGGCCAA GGACGAGTTG 
CACGACAAGA CGCCCAGTCC GATTGGGGCG GGACTGCCAT TGCTGCAGCG CCTCCGGCTC 
CTCAAGGAGA AGCAGGATCG CGAAGAGCGA GCTGTTAAGT CCACACCACC ACAGAAATCT 
CCACCTCACT CACATGTAAC GTGTACGTTA TCGCCGCAGG AATCGATCCA GGAGGAGCCC 
GAACGCGAGT TCAGCGAAGG CTTTCCCCTG ATCCAGCGAC TCCAGCAGTT GAAAATTAAG 
AATGAGCCGC AGGCAAACGC CGCCGAACCC GGCGTCGTCA TGGTCAACAC ACCGCCCAAC 
ATTAGCAGCA AAATTGATTC GCATTTCGCT GCCAGCCAGG CAGGTGCATC GCATCCTGGC 
CCAAATGGCG GAGCCATCGG CATCTCGGCG CCGGGACAAT CGCTGACGGT GGCCCAGATC 
AAGCCCATCA TGAAGGTGTC CTTCAAGCAG AAGATCCAGC AGATGCAGGG TGGCGGCGGA 
GGAGCCAGCA GTTCACCGGG TCCAAGCACG GGAGCCATAG CCAAGAAGGA GCCACCCAAG 
TCACTGGCTC TGGTGGCCAA GCACGCTACA TCCGAGGATC CCGTCGCCGC AGCCGGATTG 
GGTTTGGGAT CTGGTTCTAG TTCCGGCATT GCCACCATAT CGAAGAGGGT AGTCAAACCT 
CAAAGCCATC GGATGGCTAC GCCGCTGCAA TCCGACACCG ACACCGATGG CACGCCCATC 
AAACCGTGGT CCAAGCTGAA ACTGGCCACC CTGATGTCCT CCAGCTACAC GAGCTTAACC 
AACTGTTCGC CGGATGATCT GGCCTCACCG CTGAAGAACT ACTCGCTGAG CAACATACCG 
CAGCAGATGG AGTCGCATAC GCGACCACGT CACCATAGCG CCAGAAGTAG TTCGAGATCA 
CCGCGTCATG GACACCACCA TCACCATCAT CATGGCCACC ACCAGCACGG ACAGCTTCAG 
GGGCCAAGCT CCACGTGCAC CAACGGCTCC ACGACCAGCC AGGTGAACCA GGTGACGGCG 
AGTGCAAAGC GTGATTGCTT GCGTCTCCAG AAGCCGGAAC AGCACTTGCC CGACGGCGAA 
CTGACCGAAC TGGGCGGCAA TGGACGGAGG AAGCTCTACC AAAGCGTGTT CGATCTGTCG 
CCGGAATACT GCGGACTGCC GTTTGTCAAG CGATTGAAGA TCCTCAATGA GCGCCAGAAG 
CTTGCGGAAC TGGAGCGGGC ATTGCAAACC CGTAGCTTTA GCCTCGACTG CTCCAAGTCC 
GGGCCGGATA GCAAGGTGCC CATAACGGAG TCCTTGTATC GATGTTATAG CGACACTTCC 
GGCATCTACT CGCAGTTCCT CAGCACGTAC GAATCAACCA CCAGCTCCAC CTCGATATCC 
ACCAATACTT CCGCCTCTGC ATCGGCATCC GCCTCCGCAT CCGCATCCGC ATCGGATAGC 
AACTCCAGGC AATTGCAATA CGTACCCCTG CCACTCAGTC CCGAATCGAA TGAGACCATG 
GAGCGGCGCA AGCTGAAGAG CATACTCAAG AAACTCCAGA TGGGCGGTGG TCAGCAGGAG 
GAGGCGGGGC CGGGCACGGG AACGGGAGCG GGAGCGGTAA TGGGAACGGG GTCAAAGGTC 
AACTCCAGTG CCAGCCAAAA GGGGCAGGGC CTGCCGGCGG AGCCGACCTT GGAAGGCCCG 
CCCGCCAGTG CCAAGGAGGA GGAATCTCCC TTCGGCGGTG CTGCGGCGAT CGGTTCGGTG 
AATAATGCTT GCGGTAACGG TAACGGTAAC GGTAGCGTTA GTGGTAACGG TAACGGTAGC 
GGTACGGTAA ACGATAGCAG TGCTAATTCG AGTTCGCATC CGAGCAATAA CGGCAACAGT 
GCGTATACGT GTCCTCCTCC ACCGTCAGTG GCTTTTCCGT TTCCGGTACC CATTGCACCA 
GCATCCGGCT CAGTTCCACT ACCATTGGAG CCCGGAGTGT CGAATGTCTG GTCGCCGACG 
TTGACGTTGG TAACGCCCAC GAATTCGCCG CAGGAGAGCT TCGCGTTTCC TGCCCAACTG 
CAGGGGGAGT TGGGCGGACA CGGAACCGGG CGCGGACTCG TTGGTGGCGT TGGCAGCGCC 
TCAGCGTCGA TGTTGCCAGC GGGAGCGGGA GCAAGTGCAT CCGCATCCGC ATCCACGTCC 
ACGTCCGCCT CGTATGTCGT CGAGTGCTCA GCGTCGTCGA ATCAAATCCT CCATGCCCAG 
TCCACCACTG ATTTGAATTT GAATTTGCAG CTCAGGCAGA ACACGACCAG TTCGATGGGC 
GGACCGGGTC CAGCGCAACG GATTGAAGGC TTCCCGGAGG CACAGGACTA CTTCAATCAG 
ATCCTGAACG GCATCAATCA CGTGATCAAG ACGCACATGA ACGAGATGCA CTCCAAGTTC 
GAGACGCAAT TCTCCAGCAT GGCTGGTGAG GTGCGACGAC GCGACGCTAT CATTGCCCAG 
CTGCAGCTCA AGCTGCGCTC CATAGAGGGC AAGTCCTCGG CGGCACCGAC ATCCTCATCC 
TCCTCCGCAC TGGTTCTGCC CATCAGCCGG CGCCAGAAGA AGATGCCCCA GCTGTCGGTG 
GACGACGATG AGCCGCCGGA GGAGGAGGAC AACAGCAGTT CGGGTTCTTC GGCGGAACTT 
TTGTTTATGC GAGGTGACTC CCTGGACACG GTGTTCACCT CATCGCCGCC CATTCAAGGG 
GGCAGGCGCA GCACATCGCC GGGTCCTAGT CGTCATCATG CGGCCTCGGC TAACGCCCTG 
AACTGTCCGA GCAGCTACTA CGGAGGACCC GGATCCCTGA GCTCACGCCA CGCCCAGAGC 
CACCCCAGCT TCTATTCCGG CCAGGGAAAT GGACGGAACA GCGGGCGCAG CAGTGGATAT 
CGCGAGTGGA GCGGCGCAGC GGAGAGCGGA CACCTGGTCA GTGGGAGTGG ATCGAGCGGC 
GCGGTCATGG TGGCCGATGT CACGGGCAAT CTGGCCCGCC TGTCGGACAG CGTGATCCTC 
GACATTGGCG AGAGTTCCAG TTCGAGCTCC TCGTCCAGCA AGATCAACAT GGCCGAAGTG 
GATGACGACG AGGAGGAGGA GGATCCGAGG GTGCGGGATG CTCGAAACGA GGAGGACATA 
GACGATGGCG AAGGTGGTTT GGCGAGCCAC AACGACTGGG AGGTGCAGAT GCTGGCCGCC 
GAGATGGAAC GGCAGGAACG GAAGCGTGGT CACTCCCTAT CCGACAATCT GGGCGAACTG 
AAGCACTGCA GCACGTTTCT GCGGCGACGC CGGAAGTTCA GCGACACGGA AACGGAATTT 
AGTGAGACGG ACATGGAGGA GCAGTTGGCC AGATCGGGCA GCGGCCCGAT GGAGCCAAGC 
GGATCGGCAC CATCTAGCTC TGGAGCCGGT GGCTCCACAT CCGCTGGACA TCAGAGCGGC 
AGCCAGCGAC CGAGGGCCTC CAGTCTGGAT CAGTTCAATC TGCGCTACGG CATCGGGCGC 
GGAATCTTTA AAGCAATGAG CATCGATCGT GATAAAGACA AGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cngl ( NM_001258762.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245691.1 Drosophila melanogaster cyclic nucleotide-gated ion channel-like, transcript variant E (Cngl), mRNA.

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CloneID	OFa02728
Clone ID Related Accession (Same CDS sequence)	NM_001258762.3
Accession Version	NM_001258762.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cyclic nucleotide-gated ion channel-like, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258762.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAACTGA GCGTGAGCGC GTATCGGGCG CATGCATCCG CACACAAGAA GATCCTCTCC 
AGCGGCTATC ACTCACAGCT CAACTACGGC AGCACTGGAG CGGCAGCGGC ATCCTCCTCG 
TCGTCGGGGG CCACTGCAAC GGGCCTATAT ACGATGGAAG CGCACGAGCA CGAAGCGGGC 
AAGTTTGTCA AGGATGTGGC CCGTCAGGTG CACGACTGCA ACAGCGACAC GGATGTCATC 
AGTCCCACGG GCACCAGTAT CTCCGGTGGC GGAGGAGGCG GCGGAGCAGG CGGTGCCGGC 
GGATCCGGTG GTGCTGGCGG ACACAGCGAC GGTGGCTATC ACAAGCGGCA CCACCACAAG 
ATGGTCCGCG ATGACGACAA CAACAGTGCG GCGCACTCAT CGGACAGCAA GAGTCCCAGC 
CAGCGGAAAT CGCGTATCGG AGATGGAGCT TCAGATCCAG ATTCCGATTG GACACGATCG 
AACCAACGCT GGATGAAGCT ACGCACCACC GTGCAGATCT CATCGGCGAT ACAGAAGAAA 
CCACCGCTCA AGCGCGAAGA CTCCTTCCTG AAGCGTTTCT CGACTAGACA GATACCCGAA 
ACACAGGAAA CTGTTGAAGA TACGGGTTCA GAAAGTGCCT CTGGTGACGT CGACAAGAGT 
GTTAAACGAC GGCGACGCTA TCTGCAGAAA CGACGATCCG TTGTTAATCC AGATGAAAAT 
TTTTACTTCT ATTGGTTAAT GATGTTAACT GTATGTGTTC TATATAATCT ATGGACCCTA 
ATTGTGAGGC AGAGCTTTCC TGAACTGCAG CAATCTGTGC CCACGTTTTG GCTCATCTGC 
GATTCGATGA CAGATGTTGT ATTTATCCTA GATATAATAG TTCAATTACG CACAGGCTAT 
CTGGAGCAGG GCCTAATGGT ATACGACGAC AGGAAGCTGG CCTGTCACTA CGTTCACTCG 
CGCGACTTTA TCTTCGATAT GATAGCGCTG ATACCATTGG ATTTGCTGCA GCTCAAGATG 
GGCACACATC CGCTCTTGCG TTTTACGCGC TTTTTTAAAG TTTATCGATC TGTGAGATTT 
TATTACATCG TAGAAAGTAG AACAGTTTGG CCAAATTTAT GGCGTGTTGT TAACCTAATT 
CATATCCTGT TAATACTGGC ACACTGGTTC GGTTGTTTCT ATTTTTTACT CTCCGAGGCG 
GAGGGCTTTC AGGGCGACTG GGTGTATCCG TATCGACCCG GGGACTATGC CACCCTAACG 
CGAAAGTATC TGGGCAGCCT GTACTGGTCG ACCCTGACAC TGACGACCAT TGGGGACCTG 
CCCACACCCG AAACGAATGC AGAATATATT TTTACGATCG TTAGCTATTT GATTGGTGTT 
TTTATCTTCG CCACCATTGT GGGCCAAGTG GGCAATGTGA TAACGAACCG AAATGCGAAT 
CGCCTGGAGT TCGAGCGCCT TCTGGATGGG GCCAAGACCT ATATGCGGCA CCACAAGGTG 
CCCGGAGGGA TGAAGCGGCG CGTGCTGCGA TGGTACGACT ATAGCTGGTC CCGCGGCAGG 
ATACAGGGTG GCGGTGACAT CAATACCGCA CTGGGCCTCC TGCCCGACAA GCTGAAAACC 
GAATTGGCCT TACATGTCAA CCTGAGCGTT CTGAAGAAGG TGACCATATT CCAAGAGTGC 
CAGCCCGAGT TCCTTCACGA TCTCGTGCTC AAGATGAAGG CCTACATCTT TACGCCGGGC 
GACTCCATTT GCCGTAAGGG CGAGGTGGCC CGCGAGATGT TCATCATTGC CGATGGCATT 
CTGGAGGTGC TGAGCGAGAC GGGCAAGGTC CTAACCACCA TGAAGGCTGG CGATTTTTTT 
GGCGAAATCG GCATCCTCAA TCTGGACGGG CTTAACAAAC GCACAGCGGA TGTGCGCTCC 
GTGGGCTACT CGGAGCTCTT CTCTCTGTCC CGCGAGGATG TCCTGGCGGC CATGAAGGAC 
TACCCCGATG CCCAGGAGAT TCTGCAGACC CTGGGCCGCA AGCGGCTAAT GGAGGTGCGT 
TGCGTGAACA AAAAGTACGC CAAGGCGCAG AGTGACAAGG AGGCGGCCGC CTATGCGGCA 
GCTCATCCGC ACCACCATCA GAGCCATCAC CAGGGTCAGG TGCACCAGAG CGACAGCAGC 
GAGAACAGTG CCTCCAAGAA GATCGTGGAC AAGCTGAAGC ACGACGTCAA GGGATTCCGC 
AACGTGCTGA AGAAGTCCAG GACTTCGCGC AAAAGCGATG AATCCTTGGA GATGCAGCCA 
CTGCACAACA CCTCGCCGCG CGGCAGCAAG ATCCTGCTGA AGCGCATGTC ACGCGTTCGA 
TCGGACGAAA AGGATGCGGA TAGCGCCGAG GCCAAGGACG AGTTGCACGA CAAGACGCCC 
AGTCCGATTG GGGCGGGACT GCCATTGCTG CAGCGCCTCC GGCTCCTCAA GGAGAAGCAG 
GATCGCGAAG AGCGAGCTGT TAAGTCCACA CCACCACAGA AATCTCCACC TCACTCACAT 
GTAACGTGTA CGTTATCGCC GCAGGAATCG ATCCAGGAGG AGCCCGAACG CGAGTTCAGC 
GAAGGCTTTC CCCTGATCCA GCGACTCCAG CAGTTGAAAA TTAAGAATGA GCCGCAGGCA 
AACGCCGCCG AACCCGGCGT CGTCATGGTC AACACACCGC CCAACATTAG CAGCAAAATT 
GATTCGCATT TCGCTGCCAG CCAGGCAGGT GCATCGCATC CTGGCCCAAA TGGCGGAGCC 
ATCGGCATCT CGGCGCCGGG ACAATCGCTG ACGGTGGCCC AGATCAAGCC CATCATGAAG 
GTGTCCTTCA AGCAGAAGAT CCAGCAGATG CAGGGTGGCG GCGGAGGAGC CAGCAGTTCA 
CCGGGTCCAA GCACGGGAGC CATAGCCAAG AAGGAGCCAC CCAAGTCACT GGCTCTGGTG 
GCCAAGCACG CTACATCCGA GGATCCCGTC GCCGCAGCCG GATTGGGTTT GGGATCTGGT 
TCTAGTTCCG GCATTGCCAC CATATCGAAG AGGGTAGTCA AACCTCAAAG CCATCGGATG 
GCTACGCCGC TGCAATCCGA CACCGACACC GATGGCACGC CCATCAAACC GTGGTCCAAG 
CTGAAACTGG CCACCCTGAT GTCCTCCAGC TACACGAGCT TAACCAACTG TTCGCCGGAT 
GATCTGGCCT CACCGCTGAA GAACTACTCG CTGAGCAACA TACCGCAGCA GATGGAGTCG 
CATACGCGAC CACGTCACCA TAGCGCCAGA AGTAGTTCGA GATCACCGCG TCATGGACAC 
CACCATCACC ATCATCATGG CCACCACCAG CACGGACAGC TTCAGGGGCC AAGCTCCACG 
TGCACCAACG GCTCCACGAC CAGCCAGGTG AACCAGGTGA CGGCGAGTGC AAAGCGTGAT 
TGCTTGCGTC TCCAGAAGCC GGAACAGCAC TTGCCCGACG GCGAACTGAC CGAACTGGGC 
GGCAATGGAC GGAGGAAGCT CTACCAAAGC GTGTTCGATC TGTCGCCGGA ATACTGCGGA 
CTGCCGTTTG TCAAGCGATT GAAGATCCTC AATGAGCGCC AGAAGCTTGC GGAACTGGAG 
CGGGCATTGC AAACCCGTAG CTTTAGCCTC GACTGCTCCA AGTCCGGGCC GGATAGCAAG 
GTGCCCATAA CGGAGTCCTT GTATCGATGT TATAGCGACA CTTCCGGCAT CTACTCGCAG 
TTCCTCAGCA CGTACGAATC AACCACCAGC TCCACCTCGA TATCCACCAA TACTTCCGCC 
TCTGCATCGG CATCCGCCTC CGCATCCGCA TCCGCATCGG ATAGCAACTC CAGGCAATTG 
CAATACGTAC CCCTGCCACT CAGTCCCGAA TCGAATGAGA CCATGGAGCG GCGCAAGCTG 
AAGAGCATAC TCAAGAAACT CCAGATGGGC GGTGGTCAGC AGGAGGAGGC GGGGCCGGGC 
ACGGGAACGG GAGCGGGAGC GGTAATGGGA ACGGGGTCAA AGGTCAACTC CAGTGCCAGC 
CAAAAGGGGC AGGGCCTGCC GGCGGAGCCG ACCTTGGAAG GCCCGCCCGC CAGTGCCAAG 
GAGGAGGAAT CTCCCTTCGG CGGTGCTGCG GCGATCGGTT CGGTGAATAA TGCTTGCGGT 
AACGGTAACG GTAACGGTAG CGTTAGTGGT AACGGTAACG GTAGCGGTAC GGTAAACGAT 
AGCAGTGCTA ATTCGAGTTC GCATCCGAGC AATAACGGCA ACAGTGCGTA TACGTGTCCT 
CCTCCACCGT CAGTGGCTTT TCCGTTTCCG GTACCCATTG CACCAGCATC CGGCTCAGTT 
CCACTACCAT TGGAGCCCGG AGTGTCGAAT GTCTGGTCGC CGACGTTGAC GTTGGTAACG 
CCCACGAATT CGCCGCAGGA GAGCTTCGCG TTTCCTGCCC AACTGCAGGG GGAGTTGGGC 
GGACACGGAA CCGGGCGCGG ACTCGTTGGT GGCGTTGGCA GCGCCTCAGC GTCGATGTTG 
CCAGCGGGAG CGGGAGCAAG TGCATCCGCA TCCGCATCCA CGTCCACGTC CGCCTCGTAT 
GTCGTCGAGT GCTCAGCGTC GTCGAATCAA ATCCTCCATG CCCAGTCCAC CACTGATTTG 
AATTTGAATT TGCAGCTCAG GCAGAACACG ACCAGTTCGA TGGGCGGACC GGGTCCAGCG 
CAACGGATTG AAGGCTTCCC GGAGGCACAG GACTACTTCA ATCAGATCCT GAACGGCATC 
AATCACGTGA TCAAGACGCA CATGAACGAG ATGCACTCCA AGTTCGAGAC GCAATTCTCC 
AGCATGGCTG GTGAGGTGCG ACGACGCGAC GCTATCATTG CCCAGCTGCA GCTCAAGCTG 
CGCTCCATAG AGGGCAAGTC CTCGGCGGCA CCGACATCCT CATCCTCCTC CGCACTGGTT 
CTGCCCATCA GCCGGCGCCA GAAGAAGATG CCCCAGCTGT CGGTGGACGA CGATGAGCCG 
CCGGAGGAGG AGGACAACAG CAGTTCGGGT TCTTCGGCGG AACTTTTGTT TATGCGAGGT 
GACTCCCTGG ACACGGTGTT CACCTCATCG CCGCCCATTC AAGGGGGCAG GCGCAGCACA 
TCGCCGGGTC CTAGTCGTCA TCATGCGGCC TCGGCTAACG CCCTGAACTG TCCGAGCAGC 
TACTACGGAG GACCCGGATC CCTGAGCTCA CGCCACGCCC AGAGCCACCC CAGCTTCTAT 
TCCGGCCAGG GAAATGGACG GAACAGCGGG CGCAGCAGTG GATATCGCGA GTGGAGCGGC 
GCAGCGGAGA GCGGACACCT GGTCAGTGGG AGTGGATCGA GCGGCGCGGT CATGGTGGCC 
GATGTCACGG GCAATCTGGC CCGCCTGTCG GACAGCGTGA TCCTCGACAT TGGCGAGAGT 
TCCAGTTCGA GCTCCTCGTC CAGCAAGATC AACATGGCCG AAGTGGATGA CGACGAGGAG 
GAGGAGGATC CGAGGGTGCG GGATGCTCGA AACGAGGAGG ACATAGACGA TGGCGAAGGT 
GGTTTGGCGA GCCACAACGA CTGGGAGGTG CAGATGCTGG CCGCCGAGAT GGAACGGCAG 
GAACGGAAGC GTGGTCACTC CCTATCCGAC AATCTGGGCG AACTGAAGCA CTGCAGCACG 
TTTCTGCGGC GACGCCGGAA GTTCAGCGAC ACGGAAACGG AATTTAGTGA GACGGACATG 
GAGGAGCAGT TGGCCAGATC GGGCAGCGGC CCGATGGAGC CAAGCGGATC GGCACCATCT 
AGCTCTGGAG CCGGTGGCTC CACATCCGCT GGACATCAGA GCGGCAGCCA GCGACCGAGG 
GCCTCCAGTC TGGATCAGTT CAATCTGCGC TACGGCATCG GGCGCGGAAT CTTTAAAGCA 
ATGAGCATCG ATCGTGATAA AGACAAGCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245691.1
CDS	2250..8162
Translation	MKLSVSAYRAHASAHKKILSSGYHSQLNYGSTGAAAASSSSSGATATGLYTMEAHEHEAGKFVKDVARQVHDCNSDTDVISPTGTSISGGGGGGGAGGAGGSGGAGGHSDGGYHKRHHHKMVRDDDNNSAAHSSDSKSPSQRKSRIGDGASDPDSDWTRSNQRWMKLRTTVQISSAIQKKPPLKREDSFLKRFSTRQIPETQETVEDTGSESASGDVDKSVKRRRRYLQKRRSVVNPDENFYFYWLMMLTVCVLYNLWTLIVRQSFPELQQSVPTFWLICDSMTDVVFILDIIVQLRTGYLEQGLMVYDDRKLACHYVHSRDFIFDMIALIPLDLLQLKMGTHPLLRFTRFFKVYRSVRFYYIVESRTVWPNLWRVVNLIHILLILAHWFGCFYFLLSEAEGFQGDWVYPYRPGDYATLTRKYLGSLYWSTLTLTTIGDLPTPETNAEYIFTIVSYLIGVFIFATIVGQVGNVITNRNANRLEFERLLDGAKTYMRHHKVPGGMKRRVLRWYDYSWSRGRIQGGGDINTALGLLPDKLKTELALHVNLSVLKKVTIFQECQPEFLHDLVLKMKAYIFTPGDSICRKGEVAREMFIIADGILEVLSETGKVLTTMKAGDFFGEIGILNLDGLNKRTADVRSVGYSELFSLSREDVLAAMKDYPDAQEILQTLGRKRLMEVRCVNKKYAKAQSDKEAAAYAAAHPHHHQSHHQGQVHQSDSSENSASKKIVDKLKHDVKGFRNVLKKSRTSRKSDESLEMQPLHNTSPRGSKILLKRMSRVRSDEKDADSAEAKDELHDKTPSPIGAGLPLLQRLRLLKEKQDREERAVKSTPPQKSPPHSHVTCTLSPQESIQEEPEREFSEGFPLIQRLQQLKIKNEPQANAAEPGVVMVNTPPNISSKIDSHFAASQAGASHPGPNGGAIGISAPGQSLTVAQIKPIMKVSFKQKIQQMQGGGGGASSSPGPSTGAIAKKEPPKSLALVAKHATSEDPVAAAGLGLGSGSSSGIATISKRVVKPQSHRMATPLQSDTDTDGTPIKPWSKLKLATLMSSSYTSLTNCSPDDLASPLKNYSLSNIPQQMESHTRPRHHSARSSSRSPRHGHHHHHHHGHHQHGQLQGPSSTCTNGSTTSQVNQVTASAKRDCLRLQKPEQHLPDGELTELGGNGRRKLYQSVFDLSPEYCGLPFVKRLKILNERQKLAELERALQTRSFSLDCSKSGPDSKVPITESLYRCYSDTSGIYSQFLSTYESTTSSTSISTNTSASASASASASASASDSNSRQLQYVPLPLSPESNETMERRKLKSILKKLQMGGGQQEEAGPGTGTGAGAVMGTGSKVNSSASQKGQGLPAEPTLEGPPASAKEEESPFGGAAAIGSVNNACGNGNGNGSVSGNGNGSGTVNDSSANSSSHPSNNGNSAYTCPPPPSVAFPFPVPIAPASGSVPLPLEPGVSNVWSPTLTLVTPTNSPQESFAFPAQLQGELGGHGTGRGLVGGVGSASASMLPAGAGASASASASTSTSASYVVECSASSNQILHAQSTTDLNLNLQLRQNTTSSMGGPGPAQRIEGFPEAQDYFNQILNGINHVIKTHMNEMHSKFETQFSSMAGEVRRRDAIIAQLQLKLRSIEGKSSAAPTSSSSSALVLPISRRQKKMPQLSVDDDEPPEEEDNSSSGSSAELLFMRGDSLDTVFTSSPPIQGGRRSTSPGPSRHHAASANALNCPSSYYGGPGSLSSRHAQSHPSFYSGQGNGRNSGRSSGYREWSGAAESGHLVSGSGSSGAVMVADVTGNLARLSDSVILDIGESSSSSSSSSKINMAEVDDDEEEEDPRVRDARNEEDIDDGEGGLASHNDWEVQMLAAEMERQERKRGHSLSDNLGELKHCSTFLRRRRKFSDTETEFSETDMEEQLARSGSGPMEPSGSAPSSSGAGGSTSAGHQSGSQRPRASSLDQFNLRYGIGRGIFKAMSIDRDKDKL

Target ORF information:

RefSeq Version	NM_001258762.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cyclic nucleotide-gated ion channel-like, transcript variant E (Cngl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258762.3

ORF Insert Sequence:

ATGAAACTGA GCGTGAGCGC GTATCGGGCG CATGCATCCG CACACAAGAA GATCCTCTCC 
AGCGGCTATC ACTCACAGCT CAACTACGGC AGCACTGGAG CGGCAGCGGC ATCCTCCTCG 
TCGTCGGGGG CCACTGCAAC GGGCCTATAT ACGATGGAAG CGCACGAGCA CGAAGCGGGC 
AAGTTTGTCA AGGATGTGGC CCGTCAGGTG CACGACTGCA ACAGCGACAC GGATGTCATC 
AGTCCCACGG GCACCAGTAT CTCCGGTGGC GGAGGAGGCG GCGGAGCAGG CGGTGCCGGC 
GGATCCGGTG GTGCTGGCGG ACACAGCGAC GGTGGCTATC ACAAGCGGCA CCACCACAAG 
ATGGTCCGCG ATGACGACAA CAACAGTGCG GCGCACTCAT CGGACAGCAA GAGTCCCAGC 
CAGCGGAAAT CGCGTATCGG AGATGGAGCT TCAGATCCAG ATTCCGATTG GACACGATCG 
AACCAACGCT GGATGAAGCT ACGCACCACC GTGCAGATCT CATCGGCGAT ACAGAAGAAA 
CCACCGCTCA AGCGCGAAGA CTCCTTCCTG AAGCGTTTCT CGACTAGACA GATACCCGAA 
ACACAGGAAA CTGTTGAAGA TACGGGTTCA GAAAGTGCCT CTGGTGACGT CGACAAGAGT 
GTTAAACGAC GGCGACGCTA TCTGCAGAAA CGACGATCCG TTGTTAATCC AGATGAAAAT 
TTTTACTTCT ATTGGTTAAT GATGTTAACT GTATGTGTTC TATATAATCT ATGGACCCTA 
ATTGTGAGGC AGAGCTTTCC TGAACTGCAG CAATCTGTGC CCACGTTTTG GCTCATCTGC 
GATTCGATGA CAGATGTTGT ATTTATCCTA GATATAATAG TTCAATTACG CACAGGCTAT 
CTGGAGCAGG GCCTAATGGT ATACGACGAC AGGAAGCTGG CCTGTCACTA CGTTCACTCG 
CGCGACTTTA TCTTCGATAT GATAGCGCTG ATACCATTGG ATTTGCTGCA GCTCAAGATG 
GGCACACATC CGCTCTTGCG TTTTACGCGC TTTTTTAAAG TTTATCGATC TGTGAGATTT 
TATTACATCG TAGAAAGTAG AACAGTTTGG CCAAATTTAT GGCGTGTTGT TAACCTAATT 
CATATCCTGT TAATACTGGC ACACTGGTTC GGTTGTTTCT ATTTTTTACT CTCCGAGGCG 
GAGGGCTTTC AGGGCGACTG GGTGTATCCG TATCGACCCG GGGACTATGC CACCCTAACG 
CGAAAGTATC TGGGCAGCCT GTACTGGTCG ACCCTGACAC TGACGACCAT TGGGGACCTG 
CCCACACCCG AAACGAATGC AGAATATATT TTTACGATCG TTAGCTATTT GATTGGTGTT 
TTTATCTTCG CCACCATTGT GGGCCAAGTG GGCAATGTGA TAACGAACCG AAATGCGAAT 
CGCCTGGAGT TCGAGCGCCT TCTGGATGGG GCCAAGACCT ATATGCGGCA CCACAAGGTG 
CCCGGAGGGA TGAAGCGGCG CGTGCTGCGA TGGTACGACT ATAGCTGGTC CCGCGGCAGG 
ATACAGGGTG GCGGTGACAT CAATACCGCA CTGGGCCTCC TGCCCGACAA GCTGAAAACC 
GAATTGGCCT TACATGTCAA CCTGAGCGTT CTGAAGAAGG TGACCATATT CCAAGAGTGC 
CAGCCCGAGT TCCTTCACGA TCTCGTGCTC AAGATGAAGG CCTACATCTT TACGCCGGGC 
GACTCCATTT GCCGTAAGGG CGAGGTGGCC CGCGAGATGT TCATCATTGC CGATGGCATT 
CTGGAGGTGC TGAGCGAGAC GGGCAAGGTC CTAACCACCA TGAAGGCTGG CGATTTTTTT 
GGCGAAATCG GCATCCTCAA TCTGGACGGG CTTAACAAAC GCACAGCGGA TGTGCGCTCC 
GTGGGCTACT CGGAGCTCTT CTCTCTGTCC CGCGAGGATG TCCTGGCGGC CATGAAGGAC 
TACCCCGATG CCCAGGAGAT TCTGCAGACC CTGGGCCGCA AGCGGCTAAT GGAGGTGCGT 
TGCGTGAACA AAAAGTACGC CAAGGCGCAG AGTGACAAGG AGGCGGCCGC CTATGCGGCA 
GCTCATCCGC ACCACCATCA GAGCCATCAC CAGGGTCAGG TGCACCAGAG CGACAGCAGC 
GAGAACAGTG CCTCCAAGAA GATCGTGGAC AAGCTGAAGC ACGACGTCAA GGGATTCCGC 
AACGTGCTGA AGAAGTCCAG GACTTCGCGC AAAAGCGATG AATCCTTGGA GATGCAGCCA 
CTGCACAACA CCTCGCCGCG CGGCAGCAAG ATCCTGCTGA AGCGCATGTC ACGCGTTCGA 
TCGGACGAAA AGGATGCGGA TAGCGCCGAG GCCAAGGACG AGTTGCACGA CAAGACGCCC 
AGTCCGATTG GGGCGGGACT GCCATTGCTG CAGCGCCTCC GGCTCCTCAA GGAGAAGCAG 
GATCGCGAAG AGCGAGCTGT TAAGTCCACA CCACCACAGA AATCTCCACC TCACTCACAT 
GTAACGTGTA CGTTATCGCC GCAGGAATCG ATCCAGGAGG AGCCCGAACG CGAGTTCAGC 
GAAGGCTTTC CCCTGATCCA GCGACTCCAG CAGTTGAAAA TTAAGAATGA GCCGCAGGCA 
AACGCCGCCG AACCCGGCGT CGTCATGGTC AACACACCGC CCAACATTAG CAGCAAAATT 
GATTCGCATT TCGCTGCCAG CCAGGCAGGT GCATCGCATC CTGGCCCAAA TGGCGGAGCC 
ATCGGCATCT CGGCGCCGGG ACAATCGCTG ACGGTGGCCC AGATCAAGCC CATCATGAAG 
GTGTCCTTCA AGCAGAAGAT CCAGCAGATG CAGGGTGGCG GCGGAGGAGC CAGCAGTTCA 
CCGGGTCCAA GCACGGGAGC CATAGCCAAG AAGGAGCCAC CCAAGTCACT GGCTCTGGTG 
GCCAAGCACG CTACATCCGA GGATCCCGTC GCCGCAGCCG GATTGGGTTT GGGATCTGGT 
TCTAGTTCCG GCATTGCCAC CATATCGAAG AGGGTAGTCA AACCTCAAAG CCATCGGATG 
GCTACGCCGC TGCAATCCGA CACCGACACC GATGGCACGC CCATCAAACC GTGGTCCAAG 
CTGAAACTGG CCACCCTGAT GTCCTCCAGC TACACGAGCT TAACCAACTG TTCGCCGGAT 
GATCTGGCCT CACCGCTGAA GAACTACTCG CTGAGCAACA TACCGCAGCA GATGGAGTCG 
CATACGCGAC CACGTCACCA TAGCGCCAGA AGTAGTTCGA GATCACCGCG TCATGGACAC 
CACCATCACC ATCATCATGG CCACCACCAG CACGGACAGC TTCAGGGGCC AAGCTCCACG 
TGCACCAACG GCTCCACGAC CAGCCAGGTG AACCAGGTGA CGGCGAGTGC AAAGCGTGAT 
TGCTTGCGTC TCCAGAAGCC GGAACAGCAC TTGCCCGACG GCGAACTGAC CGAACTGGGC 
GGCAATGGAC GGAGGAAGCT CTACCAAAGC GTGTTCGATC TGTCGCCGGA ATACTGCGGA 
CTGCCGTTTG TCAAGCGATT GAAGATCCTC AATGAGCGCC AGAAGCTTGC GGAACTGGAG 
CGGGCATTGC AAACCCGTAG CTTTAGCCTC GACTGCTCCA AGTCCGGGCC GGATAGCAAG 
GTGCCCATAA CGGAGTCCTT GTATCGATGT TATAGCGACA CTTCCGGCAT CTACTCGCAG 
TTCCTCAGCA CGTACGAATC AACCACCAGC TCCACCTCGA TATCCACCAA TACTTCCGCC 
TCTGCATCGG CATCCGCCTC CGCATCCGCA TCCGCATCGG ATAGCAACTC CAGGCAATTG 
CAATACGTAC CCCTGCCACT CAGTCCCGAA TCGAATGAGA CCATGGAGCG GCGCAAGCTG 
AAGAGCATAC TCAAGAAACT CCAGATGGGC GGTGGTCAGC AGGAGGAGGC GGGGCCGGGC 
ACGGGAACGG GAGCGGGAGC GGTAATGGGA ACGGGGTCAA AGGTCAACTC CAGTGCCAGC 
CAAAAGGGGC AGGGCCTGCC GGCGGAGCCG ACCTTGGAAG GCCCGCCCGC CAGTGCCAAG 
GAGGAGGAAT CTCCCTTCGG CGGTGCTGCG GCGATCGGTT CGGTGAATAA TGCTTGCGGT 
AACGGTAACG GTAACGGTAG CGTTAGTGGT AACGGTAACG GTAGCGGTAC GGTAAACGAT 
AGCAGTGCTA ATTCGAGTTC GCATCCGAGC AATAACGGCA ACAGTGCGTA TACGTGTCCT 
CCTCCACCGT CAGTGGCTTT TCCGTTTCCG GTACCCATTG CACCAGCATC CGGCTCAGTT 
CCACTACCAT TGGAGCCCGG AGTGTCGAAT GTCTGGTCGC CGACGTTGAC GTTGGTAACG 
CCCACGAATT CGCCGCAGGA GAGCTTCGCG TTTCCTGCCC AACTGCAGGG GGAGTTGGGC 
GGACACGGAA CCGGGCGCGG ACTCGTTGGT GGCGTTGGCA GCGCCTCAGC GTCGATGTTG 
CCAGCGGGAG CGGGAGCAAG TGCATCCGCA TCCGCATCCA CGTCCACGTC CGCCTCGTAT 
GTCGTCGAGT GCTCAGCGTC GTCGAATCAA ATCCTCCATG CCCAGTCCAC CACTGATTTG 
AATTTGAATT TGCAGCTCAG GCAGAACACG ACCAGTTCGA TGGGCGGACC GGGTCCAGCG 
CAACGGATTG AAGGCTTCCC GGAGGCACAG GACTACTTCA ATCAGATCCT GAACGGCATC 
AATCACGTGA TCAAGACGCA CATGAACGAG ATGCACTCCA AGTTCGAGAC GCAATTCTCC 
AGCATGGCTG GTGAGGTGCG ACGACGCGAC GCTATCATTG CCCAGCTGCA GCTCAAGCTG 
CGCTCCATAG AGGGCAAGTC CTCGGCGGCA CCGACATCCT CATCCTCCTC CGCACTGGTT 
CTGCCCATCA GCCGGCGCCA GAAGAAGATG CCCCAGCTGT CGGTGGACGA CGATGAGCCG 
CCGGAGGAGG AGGACAACAG CAGTTCGGGT TCTTCGGCGG AACTTTTGTT TATGCGAGGT 
GACTCCCTGG ACACGGTGTT CACCTCATCG CCGCCCATTC AAGGGGGCAG GCGCAGCACA 
TCGCCGGGTC CTAGTCGTCA TCATGCGGCC TCGGCTAACG CCCTGAACTG TCCGAGCAGC 
TACTACGGAG GACCCGGATC CCTGAGCTCA CGCCACGCCC AGAGCCACCC CAGCTTCTAT 
TCCGGCCAGG GAAATGGACG GAACAGCGGG CGCAGCAGTG GATATCGCGA GTGGAGCGGC 
GCAGCGGAGA GCGGACACCT GGTCAGTGGG AGTGGATCGA GCGGCGCGGT CATGGTGGCC 
GATGTCACGG GCAATCTGGC CCGCCTGTCG GACAGCGTGA TCCTCGACAT TGGCGAGAGT 
TCCAGTTCGA GCTCCTCGTC CAGCAAGATC AACATGGCCG AAGTGGATGA CGACGAGGAG 
GAGGAGGATC CGAGGGTGCG GGATGCTCGA AACGAGGAGG ACATAGACGA TGGCGAAGGT 
GGTTTGGCGA GCCACAACGA CTGGGAGGTG CAGATGCTGG CCGCCGAGAT GGAACGGCAG 
GAACGGAAGC GTGGTCACTC CCTATCCGAC AATCTGGGCG AACTGAAGCA CTGCAGCACG 
TTTCTGCGGC GACGCCGGAA GTTCAGCGAC ACGGAAACGG AATTTAGTGA GACGGACATG 
GAGGAGCAGT TGGCCAGATC GGGCAGCGGC CCGATGGAGC CAAGCGGATC GGCACCATCT 
AGCTCTGGAG CCGGTGGCTC CACATCCGCT GGACATCAGA GCGGCAGCCA GCGACCGAGG 
GCCTCCAGTC TGGATCAGTT CAATCTGCGC TACGGCATCG GGCGCGGAAT CTTTAAAGCA 
ATGAGCATCG ATCGTGATAA AGACAAGCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cngl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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