Sp1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sp1
Entrez Gene ID	31913
Full Name	CG1343 gene product from transcript CG1343-RE
Synonyms	CG1343,D-Sp1,D-Sp8,D.Sp1,Dmel\CG1343,SP1,dSp1,sp1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 9A1-9A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_167200.4	NP_727360.1	Sp1, isoform B
NM_001103457.4	NP_001096927.2	Sp1, isoform D
NM_001272459.1	NP_001259388.1	Sp1, isoform F
NM_132351.3	NP_572579.3	Sp1, isoform E

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	sp8a	NP_998406.2
Drosophila melanogaster (fruit fly)	Sp1	NP_001259388.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These studies establish a role for Sp1 in leg-to-wing transdetermination.
Title: Sp1 modifies leg-to-wing transdetermination in Drosophila.

By simultaneously removing the function of two Sp8 orthologs, buttonhead (btd) and Sp1, during Drosophila embryogenesis, we find that adult leg development is completely abolished.
Title: Non-redundant selector and growth-promoting functions of two sister genes, buttonhead and Sp1, in Drosophila leg development.

Results show that buttonhead and Sp1 induce the formation and are required for the growth of the ventral imaginal discs.
Title: The role of buttonhead and Sp1 in the development of the ventral imaginal discs of Drosophila.

The following Sp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01184	NM_001272459.1 Latest version!	Drosophila melanogaster Sp1, transcript variant F (Sp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa03368	NM_132351.3 Latest version!	Drosophila melanogaster Sp1, transcript variant E (Sp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa03369	NM_001103457.4 Latest version!	Drosophila melanogaster Sp1, transcript variant D (Sp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa03370	NM_167200.4 Latest version!	Drosophila melanogaster Sp1, transcript variant B (Sp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sp1 ( NM_001272459.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259388.1 Drosophila melanogaster Sp1, transcript variant F (Sp1), mRNA.

Price & Availability↑

CloneID	OFa01184
Clone ID Related Accession (Same CDS sequence)	NM_001272459.1
Accession Version	NM_001272459.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sp1, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTAACGG ACATGACACC GACCGCCGGT CAGCTCTACG GATCCCAGAT TCCGGCCATG 
GGCATGAACA TCAGCAATAT CGCCACACAC TTGCAGAAGC CACAAGTTAA TCCGGATCAT 
CCCAGCTTGA GAGGAACTCC GCTGGCCATG CTGGCCGCCC AGTGCAACAA GCTATCCAAC 
AAATCCCCAC CCCCGCTGGC CGACGCCGCT GTGGGCAAGG GCTTCCATCC GTGGAAGAAG 
AGTCCCAATT CGCCGGCAGC CGGATCCAGT GGCTCCTCCG GCGGTGGTGG CGGCGGCGGT 
GGCAGTAGCG CCGGCCAGCA CTCGCCCTGC GCCATCTCAG CGGCCAGCTC GTCCAGCTCG 
AGCGGATCAA GCGGAGGACA GTCCTCCAGG AGCCTCTCAT CCAGCGCCAG CACCATGGTT 
AACATCACAG CTAGTCGCCC CCTGGCCTCC TCCTGTGCGG CGGTGGGCGG GGGCTCCACC 
GGCTCCTCCT CCTCCGCCTC TGGATCACAG TCATCCTCGA CGGCATCTGC GGTGGCAGCG 
GCCTACGGCG GTGATTTGTA CTTTCCGAAC ACATCCACCT CGAACATGGA CAACCATCAC 
ATGCATCAGG GTCTCCTGGG TAAAGTGGAG GCGGGTGCGG CTGCCTTTGG CGGTGTCTAT 
TCGCGTCATC CGTACGATTG GCCCTTCAAT GCGGTTACCC ACAAGGAGGC GGCCAGCGTG 
AATTCCGGCT GGTGGGACAT GCACAGTGCC GCCGGATCCT GGCTGGATAT GGGTGGTGCG 
GGCATGCACT CAACAATGGC CAATTATGCG AGTGAGAACT ATAGCTCCGC ATTGAGTCAT 
TCGCTGTTGG GATCGGGACA ACATTTGCTG CAGGACACCT ACAAGAGCAT GTTGCCCGGC 
CAGGGAGTTG GCGTTGGTGT GGGCGTTGGC ATGGGTGGCT TCTCCCTGCC TCACAGTTCA 
CCATCGGCGG CAGCAGCAGC TGCGGCCACA GCGGCAGCGG CGGCGGGTGG CTCCCCCCAG 
GGTGGTTCAC CATCGACACC ATCACCCAGA TCCCAGAGGC GTTATGCCGG ACGGGCCACA 
TGCGATTGCC CGAATTGCCA GGAGGCGGAA CGCCTGGGTC CCGCTGGCGT TCATCTGCGC 
AAAAAGAACA TCCATTCGTG TCACATACCT GGCTGTGGCA AGGTGTATGG AAAGACGTCA 
CATCTGAAGG CTCACCTGCG GTGGCACACC GGCGAGCGTC CCTTTGTGTG CAACTGGCTG 
TTCTGCGGCA AGCGTTTCAC ACGCTCCGAC GAGCTGCAAC GGCATTTGAG GACGCACACC 
GGCGAGAAGC GCTTTGCATG CCCCGTGTGC AATAAGCGCT TTATGCGCAG CGACCATTTG 
GCCAAGCACG TGAAGACGCA CAATGGCACG GCCAATCACC AGGCCAATGG CCACAATGGT 
GGGCTGAAGA AGGGCTCCTC GGAGTCGTGT AGCGACTCGG AGGAGGCCGC CAACCAGTCG 
GGCGAGTCCA ACGGGCTGGG CGGCGTGGGC AGTGGCCCAC AGACGGGCGG GGCCGGTGGC 
GGCGGGGGTG GCTCTAGTTC GGGCAATGGT GCTGGTACGT TAGCGGTATC GGGATCGGTG 
ACCACCGGAG CGGGAGCTGG ATCCGGTACG GGTAGCTCCA ATTCCAATTC CAATTCCGGT 
GGCTCAAGCG GTTCCGTTAG CGGTTCCGTA TCCGGTTCTG GCAGCCATCC CGGCACCCCG 
ACCTCGCTGC ACGCGCACAG CGCCAATGGC ACGTCCAGCA GCCTGCTGGG CGGCGGTCTG 
CACCTGGCCA CGCCGCACCA GATGGTCGCC GCGGGCGGTT CGCCGGTGAT GCTTCACCAG 
CAGCAGCAGC AGCAGCAACA TCAGCAGCAA CAGCAGCAAC AGCAGCAGCA GCAGCATCAA 
CAGCAGCAAC AGCAGCAACA TCAACAGCAG CAGCAGCAAC ATCAGCAGCA GCAGCAGCAA 
CACCACCAGC AACAGCAGCA GCATCATCAC CAGCAATTTG GCCAGCAGCA GCATCCCCAT 
GCCCATCACC TGCACCACCA TGCCCACCAC GCGCACCACC TGGCCACCGG CGGCTCACCC 
GGTCTGGATC CCAGCTCCCT CGTGGATATA AAGCCACCGA TGGTTTGAGG ATCGCTGGGA 
CCCTTCCTGT TCACCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259388.1
CDS	917..3097
Translation	MLTDMTPTAGQLYGSQIPAMGMNISNIATHLQKPQVNPDHPSLRGTPLAMLAAQCNKLSNKSPPPLADAAVGKGFHPWKKSPNSPAAGSSGSSGGGGGGGGSSAGQHSPCAISAASSSSSSGSSGGQSSRSLSSSASTMVNITASRPLASSCAAVGGGSTGSSSSASGSQSSSTASAVAAAYGGDLYFPNTSTSNMDNHHMHQGLLGKVEAGAAAFGGVYSRHPYDWPFNAVTHKEAASVNSGWWDMHSAAGSWLDMGGAGMHSTMANYASENYSSALSHSLLGSGQHLLQDTYKSMLPGQGVGVGVGVGMGGFSLPHSSPSAAAAAAATAAAAAGGSPQGGSPSTPSPRSQRRYAGRATCDCPNCQEAERLGPAGVHLRKKNIHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLAKHVKTHNGTANHQANGHNGGLKKGSSESCSDSEEAANQSGESNGLGGVGSGPQTGGAGGGGGGSSSGNGAGTLAVSGSVTTGAGAGSGTGSSNSNSNSGGSSGSVSGSVSGSGSHPGTPTSLHAHSANGTSSSLLGGGLHLATPHQMVAAGGSPVMLHQQQQQQQHQQQQQQQQQQQHQQQQQQQHQQQQQQHQQQQQQHHQQQQQHHHQQFGQQQHPHAHHLHHHAHHAHHLATGGSPGLDPSSLVDIKPPMV*GSLGPFLFTN

Target ORF information:

RefSeq Version	NM_001272459.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sp1, transcript variant F (Sp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272459.1

ORF Insert Sequence:

ATGTTAACGG ACATGACACC GACCGCCGGT CAGCTCTACG GATCCCAGAT TCCGGCCATG 
GGCATGAACA TCAGCAATAT CGCCACACAC TTGCAGAAGC CACAAGTTAA TCCGGATCAT 
CCCAGCTTGA GAGGAACTCC GCTGGCCATG CTGGCCGCCC AGTGCAACAA GCTATCCAAC 
AAATCCCCAC CCCCGCTGGC CGACGCCGCT GTGGGCAAGG GCTTCCATCC GTGGAAGAAG 
AGTCCCAATT CGCCGGCAGC CGGATCCAGT GGCTCCTCCG GCGGTGGTGG CGGCGGCGGT 
GGCAGTAGCG CCGGCCAGCA CTCGCCCTGC GCCATCTCAG CGGCCAGCTC GTCCAGCTCG 
AGCGGATCAA GCGGAGGACA GTCCTCCAGG AGCCTCTCAT CCAGCGCCAG CACCATGGTT 
AACATCACAG CTAGTCGCCC CCTGGCCTCC TCCTGTGCGG CGGTGGGCGG GGGCTCCACC 
GGCTCCTCCT CCTCCGCCTC TGGATCACAG TCATCCTCGA CGGCATCTGC GGTGGCAGCG 
GCCTACGGCG GTGATTTGTA CTTTCCGAAC ACATCCACCT CGAACATGGA CAACCATCAC 
ATGCATCAGG GTCTCCTGGG TAAAGTGGAG GCGGGTGCGG CTGCCTTTGG CGGTGTCTAT 
TCGCGTCATC CGTACGATTG GCCCTTCAAT GCGGTTACCC ACAAGGAGGC GGCCAGCGTG 
AATTCCGGCT GGTGGGACAT GCACAGTGCC GCCGGATCCT GGCTGGATAT GGGTGGTGCG 
GGCATGCACT CAACAATGGC CAATTATGCG AGTGAGAACT ATAGCTCCGC ATTGAGTCAT 
TCGCTGTTGG GATCGGGACA ACATTTGCTG CAGGACACCT ACAAGAGCAT GTTGCCCGGC 
CAGGGAGTTG GCGTTGGTGT GGGCGTTGGC ATGGGTGGCT TCTCCCTGCC TCACAGTTCA 
CCATCGGCGG CAGCAGCAGC TGCGGCCACA GCGGCAGCGG CGGCGGGTGG CTCCCCCCAG 
GGTGGTTCAC CATCGACACC ATCACCCAGA TCCCAGAGGC GTTATGCCGG ACGGGCCACA 
TGCGATTGCC CGAATTGCCA GGAGGCGGAA CGCCTGGGTC CCGCTGGCGT TCATCTGCGC 
AAAAAGAACA TCCATTCGTG TCACATACCT GGCTGTGGCA AGGTGTATGG AAAGACGTCA 
CATCTGAAGG CTCACCTGCG GTGGCACACC GGCGAGCGTC CCTTTGTGTG CAACTGGCTG 
TTCTGCGGCA AGCGTTTCAC ACGCTCCGAC GAGCTGCAAC GGCATTTGAG GACGCACACC 
GGCGAGAAGC GCTTTGCATG CCCCGTGTGC AATAAGCGCT TTATGCGCAG CGACCATTTG 
GCCAAGCACG TGAAGACGCA CAATGGCACG GCCAATCACC AGGCCAATGG CCACAATGGT 
GGGCTGAAGA AGGGCTCCTC GGAGTCGTGT AGCGACTCGG AGGAGGCCGC CAACCAGTCG 
GGCGAGTCCA ACGGGCTGGG CGGCGTGGGC AGTGGCCCAC AGACGGGCGG GGCCGGTGGC 
GGCGGGGGTG GCTCTAGTTC GGGCAATGGT GCTGGTACGT TAGCGGTATC GGGATCGGTG 
ACCACCGGAG CGGGAGCTGG ATCCGGTACG GGTAGCTCCA ATTCCAATTC CAATTCCGGT 
GGCTCAAGCG GTTCCGTTAG CGGTTCCGTA TCCGGTTCTG GCAGCCATCC CGGCACCCCG 
ACCTCGCTGC ACGCGCACAG CGCCAATGGC ACGTCCAGCA GCCTGCTGGG CGGCGGTCTG 
CACCTGGCCA CGCCGCACCA GATGGTCGCC GCGGGCGGTT CGCCGGTGAT GCTTCACCAG 
CAGCAGCAGC AGCAGCAACA TCAGCAGCAA CAGCAGCAAC AGCAGCAGCA GCAGCATCAA 
CAGCAGCAAC AGCAGCAACA TCAACAGCAG CAGCAGCAAC ATCAGCAGCA GCAGCAGCAA 
CACCACCAGC AACAGCAGCA GCATCATCAC CAGCAATTTG GCCAGCAGCA GCATCCCCAT 
GCCCATCACC TGCACCACCA TGCCCACCAC GCGCACCACC TGGCCACCGG CGGCTCACCC 
GGTCTGGATC CCAGCTCCCT CGTGGATATA AAGCCACCGA TGGTTTGAGG ATCGCTGGGA 
CCCTTCCTGT TCACCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sp1 ( NM_132351.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572579.3 Drosophila melanogaster Sp1, transcript variant E (Sp1), mRNA.

Price & Availability↑

CloneID	OFa03368
Clone ID Related Accession (Same CDS sequence)	NM_132351.3
Accession Version	NM_132351.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sp1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132351.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTAACGG ACATGACACC GACCGCCGGT CAGCTCTACG GATCCCAGAT TCCGGCCATG 
GGCATGAACA TCAGCAATAT CGCCACACAC TTGCAGAAGC CACAAGTTAA TCCGGATCAT 
CCCAGCTTGA GAGGAACTCC GCTGGCCATG CTGGCCGCCC AGTGCAACAA GCTATCCAAC 
AAATCCCCAC CCCCGCTGGC CGACGCCGCT GTGGGCAAGG GCTTCCATCC GTGGAAGAAG 
AGTCCCAATT CGCCGGCAGC CGGATCCAGT GGCTCCTCCG GCGGTGGTGG CGGCGGCGGT 
GGCAGTAGCG CCGGCCAGCA CTCGCCCTGC GCCATCTCAG CGGCCAGCTC GTCCAGCTCG 
AGCGGATCAA GCGGAGGACA GTCCTCCAGG AGCCTCTCAT CCAGCGCCAG CACCATGGTT 
AACATCACAG CTAGCATCTA TCCTTACAGT CGCCCCCTGG CCTCCTCCTG TGCGGCGGTG 
GGCGGGGGCT CCACCGGCTC CTCCTCCTCC GCCTCTGGAT CACAGTCATC CTCGACGGCA 
TCTGCGGTGG CAGCGGCCTA CGGCGGTGAT TTGTACTTTC CGAACACATC CACCTCGAAC 
ATGGACAACC ATCACATGCA TCAGGGTCTC CTGGGTAAAG TGGAGGCGGG TGCGGCTGCC 
TTTGGCGGTG TCTATTCGCG TCATCCGTAC GATTGGCCCT TCAATGCGGT TACCCACAAG 
GAGGCGGCCA GCGTGAATTC CGGCTGGTGG GACATGCACA GTGCCGCCGG ATCCTGGCTG 
GATATGGGTG GTGCGGGCAT GCACTCAACA ATGGCCAATT ATGCGAGTGA GAACTATAGC 
TCCGCATTGA GTCATTCGCT GTTGGGATCG GGACAACATT TGCTGCAGGA CACCTACAAG 
AGCATGTTGC CCGGCCAGGG AGTTGGCGTT GGTGTGGGCG TTGGCATGGG TGGCTTCTCC 
CTGCCTCACA GTTCACCATC GGCGGCAGCA GCAGCTGCGG CCACAGCGGC AGCGGCGGCG 
GGTGGCTCCC CCCAGGGTGG TTCACCATCG ACACCATCAC CCAGATCCCA GAGGCGTTAT 
GCCGGACGGG CCACATGCGA TTGCCCGAAT TGCCAGGAGG CGGAACGCCT GGGTCCCGCT 
GGCGTTCATC TGCGCAAAAA GAACATCCAT TCGTGTCACA TACCTGGCTG TGGCAAGGTG 
TATGGAAAGA CGTCACATCT GAAGGCTCAC CTGCGGTGGC ACACCGGCGA GCGTCCCTTT 
GTGTGCAACT GGCTGTTCTG CGGCAAGCGT TTCACACGCT CCGACGAGCT GCAACGGCAT 
TTGAGGACGC ACACCGGCGA GAAGCGCTTT GCATGCCCCG TGTGCAATAA GCGCTTTATG 
CGCAGCGACC ATTTGGCCAA GCACGTGAAG ACGCACAATG GCACGGCCAA TCACCAGGCC 
AATGGCCACA ATGGTGGGCT GAAGAAGGGC TCCTCGGAGT CGTGTAGCGA CTCGGAGGAG 
GCCGCCAACC AGTCGGGCGA GTCCAACGGG CTGGGCGGCG TGGGCAGTGG CCCACAGACG 
GGCGGGGCCG GTGGCGGCGG GGGTGGCTCT AGTTCGGGCA ATGGTGCTGG TACGTTAGCG 
GTATCGGGAT CGGTGACCAC CGGAGCGGGA GCTGGATCCG GTACGGGTAG CTCCAATTCC 
AATTCCAATT CCGGTGGCTC AAGCGGTTCC GTTAGCGGTT CCGTATCCGG TTCTGGCAGC 
CATCCCGGCA CCCCGACCTC GCTGCACGCG CACAGCGCCA ATGGCACGTC CAGCAGCCTG 
CTGGGCGGCG GTCTGCACCT GGCCACGCCG CACCAGATGG TCGCCGCGGG CGGTTCGCCG 
GTGATGCTTC ACCAGCAGCA GCAGCAGCAG CAACATCAGC AGCAACAGCA GCAACAGCAG 
CAGCAGCAGC ATCAACAGCA GCAACAGCAG CAACATCAAC AGCAGCAGCA GCAACATCAG 
CAGCAGCAGC AGCAACACCA CCAGCAACAG CAGCAGCATC ATCACCAGCA ATTTGGCCAG 
CAGCAGCATC CCCATGCCCA TCACCTGCAC CACCATGCCC ACCACGCGCA CCACCTGGCC 
ACCGGCGGCT CACCCGGTCT GGATCCCAGC TCCCTCGTGG ATATAAAGCC ACCGATGGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572579.3
CDS	501..2663
Translation	MLTDMTPTAGQLYGSQIPAMGMNISNIATHLQKPQVNPDHPSLRGTPLAMLAAQCNKLSNKSPPPLADAAVGKGFHPWKKSPNSPAAGSSGSSGGGGGGGGSSAGQHSPCAISAASSSSSSGSSGGQSSRSLSSSASTMVNITASIYPYSRPLASSCAAVGGGSTGSSSSASGSQSSSTASAVAAAYGGDLYFPNTSTSNMDNHHMHQGLLGKVEAGAAAFGGVYSRHPYDWPFNAVTHKEAASVNSGWWDMHSAAGSWLDMGGAGMHSTMANYASENYSSALSHSLLGSGQHLLQDTYKSMLPGQGVGVGVGVGMGGFSLPHSSPSAAAAAAATAAAAAGGSPQGGSPSTPSPRSQRRYAGRATCDCPNCQEAERLGPAGVHLRKKNIHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLAKHVKTHNGTANHQANGHNGGLKKGSSESCSDSEEAANQSGESNGLGGVGSGPQTGGAGGGGGGSSSGNGAGTLAVSGSVTTGAGAGSGTGSSNSNSNSGGSSGSVSGSVSGSGSHPGTPTSLHAHSANGTSSSLLGGGLHLATPHQMVAAGGSPVMLHQQQQQQQHQQQQQQQQQQQHQQQQQQQHQQQQQQHQQQQQQHHQQQQQHHHQQFGQQQHPHAHHLHHHAHHAHHLATGGSPGLDPSSLVDIKPPMV

Target ORF information:

RefSeq Version	NM_132351.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sp1, transcript variant E (Sp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132351.3

ORF Insert Sequence:

ATGTTAACGG ACATGACACC GACCGCCGGT CAGCTCTACG GATCCCAGAT TCCGGCCATG 
GGCATGAACA TCAGCAATAT CGCCACACAC TTGCAGAAGC CACAAGTTAA TCCGGATCAT 
CCCAGCTTGA GAGGAACTCC GCTGGCCATG CTGGCCGCCC AGTGCAACAA GCTATCCAAC 
AAATCCCCAC CCCCGCTGGC CGACGCCGCT GTGGGCAAGG GCTTCCATCC GTGGAAGAAG 
AGTCCCAATT CGCCGGCAGC CGGATCCAGT GGCTCCTCCG GCGGTGGTGG CGGCGGCGGT 
GGCAGTAGCG CCGGCCAGCA CTCGCCCTGC GCCATCTCAG CGGCCAGCTC GTCCAGCTCG 
AGCGGATCAA GCGGAGGACA GTCCTCCAGG AGCCTCTCAT CCAGCGCCAG CACCATGGTT 
AACATCACAG CTAGCATCTA TCCTTACAGT CGCCCCCTGG CCTCCTCCTG TGCGGCGGTG 
GGCGGGGGCT CCACCGGCTC CTCCTCCTCC GCCTCTGGAT CACAGTCATC CTCGACGGCA 
TCTGCGGTGG CAGCGGCCTA CGGCGGTGAT TTGTACTTTC CGAACACATC CACCTCGAAC 
ATGGACAACC ATCACATGCA TCAGGGTCTC CTGGGTAAAG TGGAGGCGGG TGCGGCTGCC 
TTTGGCGGTG TCTATTCGCG TCATCCGTAC GATTGGCCCT TCAATGCGGT TACCCACAAG 
GAGGCGGCCA GCGTGAATTC CGGCTGGTGG GACATGCACA GTGCCGCCGG ATCCTGGCTG 
GATATGGGTG GTGCGGGCAT GCACTCAACA ATGGCCAATT ATGCGAGTGA GAACTATAGC 
TCCGCATTGA GTCATTCGCT GTTGGGATCG GGACAACATT TGCTGCAGGA CACCTACAAG 
AGCATGTTGC CCGGCCAGGG AGTTGGCGTT GGTGTGGGCG TTGGCATGGG TGGCTTCTCC 
CTGCCTCACA GTTCACCATC GGCGGCAGCA GCAGCTGCGG CCACAGCGGC AGCGGCGGCG 
GGTGGCTCCC CCCAGGGTGG TTCACCATCG ACACCATCAC CCAGATCCCA GAGGCGTTAT 
GCCGGACGGG CCACATGCGA TTGCCCGAAT TGCCAGGAGG CGGAACGCCT GGGTCCCGCT 
GGCGTTCATC TGCGCAAAAA GAACATCCAT TCGTGTCACA TACCTGGCTG TGGCAAGGTG 
TATGGAAAGA CGTCACATCT GAAGGCTCAC CTGCGGTGGC ACACCGGCGA GCGTCCCTTT 
GTGTGCAACT GGCTGTTCTG CGGCAAGCGT TTCACACGCT CCGACGAGCT GCAACGGCAT 
TTGAGGACGC ACACCGGCGA GAAGCGCTTT GCATGCCCCG TGTGCAATAA GCGCTTTATG 
CGCAGCGACC ATTTGGCCAA GCACGTGAAG ACGCACAATG GCACGGCCAA TCACCAGGCC 
AATGGCCACA ATGGTGGGCT GAAGAAGGGC TCCTCGGAGT CGTGTAGCGA CTCGGAGGAG 
GCCGCCAACC AGTCGGGCGA GTCCAACGGG CTGGGCGGCG TGGGCAGTGG CCCACAGACG 
GGCGGGGCCG GTGGCGGCGG GGGTGGCTCT AGTTCGGGCA ATGGTGCTGG TACGTTAGCG 
GTATCGGGAT CGGTGACCAC CGGAGCGGGA GCTGGATCCG GTACGGGTAG CTCCAATTCC 
AATTCCAATT CCGGTGGCTC AAGCGGTTCC GTTAGCGGTT CCGTATCCGG TTCTGGCAGC 
CATCCCGGCA CCCCGACCTC GCTGCACGCG CACAGCGCCA ATGGCACGTC CAGCAGCCTG 
CTGGGCGGCG GTCTGCACCT GGCCACGCCG CACCAGATGG TCGCCGCGGG CGGTTCGCCG 
GTGATGCTTC ACCAGCAGCA GCAGCAGCAG CAACATCAGC AGCAACAGCA GCAACAGCAG 
CAGCAGCAGC ATCAACAGCA GCAACAGCAG CAACATCAAC AGCAGCAGCA GCAACATCAG 
CAGCAGCAGC AGCAACACCA CCAGCAACAG CAGCAGCATC ATCACCAGCA ATTTGGCCAG 
CAGCAGCATC CCCATGCCCA TCACCTGCAC CACCATGCCC ACCACGCGCA CCACCTGGCC 
ACCGGCGGCT CACCCGGTCT GGATCCCAGC TCCCTCGTGG ATATAAAGCC ACCGATGGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sp1 ( NM_001103457.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001096927.2 Drosophila melanogaster Sp1, transcript variant D (Sp1), mRNA.

Price & Availability↑

CloneID	OFa03369
Clone ID Related Accession (Same CDS sequence)	NM_001103457.4
Accession Version	NM_001103457.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sp1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001103457.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTAACGG ACATGACACC GACCGCCGGT CAGCTCTACG GATCCCAGAT TCCGGCCATG 
GGCATGAACA TCAGCAATAT CGCCACACAC TTGCAGAAGC CACAAGTTAA TCCGGATCAT 
CCCAGCTTGA GAGGAACTCC GCTGGCCATG CTGGCCGCCC AGTGCAACAA GCTATCCAAC 
AAATCCCCAC CCCCGCTGGC CGACGCCGCT GTGGGCAAGG GCTTCCATCC GTGGAAGAAG 
AGTCCCAATT CGCCGGCAGC CGGATCCAGT GGCTCCTCCG GCGGTGGTGG CGGCGGCGGT 
GGCAGTAGCG CCGGCCAGCA CTCGCCCTGC GCCATCTCAG CGGCCAGCTC GTCCAGCTCG 
AGCGGATCAA GCGGAGGACA GTCCTCCAGG AGCCTCTCAT CCAGCGCCAG CACCATGGTT 
AACATCACAG CTAGTCGCCC CCTGGCCTCC TCCTGTGCGG CGGTGGGCGG GGGCTCCACC 
GGCTCCTCCT CCTCCGCCTC TGGATCACAG TCATCCTCGA CGGCATCTGC GGTGGCAGCG 
GCCTACGGCG GTGATTTGTA CTTTCCGAAC ACATCCACCT CGAACATGGA CAACCATCAC 
ATGCATCAGG GTCTCCTGGG TAAAGTGGAG GCGGGTGCGG CTGCCTTTGG CGGTGTCTAT 
TCGCGTCATC CGTACGATTG GCCCTTCAAT GCGGTTACCC ACAAGGAGGC GGCCAGCGTG 
AATTCCGGCT GGTGGGACAT GCACAGTGCC GCCGGATCCT GGCTGGATAT GGGTGGTGCG 
GGCATGCACT CAACAATGGC CAATTATGCG AGTGAGAACT ATAGCTCCGC ATTGAGTCAT 
TCGCTGTTGG GATCGGGACA ACATTTGCTG CAGGACACCT ACAAGAGCAT GTTGCCCGGC 
CAGGGAGTTG GCGTTGGTGT GGGCGTTGGC ATGGGTGGCT TCTCCCTGCC TCACAGTTCA 
CCATCGGCGG CAGCAGCAGC TGCGGCCACA GCGGCAGCGG CGGCGGGTGG CTCCCCCCAG 
GGTGGTTCAC CATCGACACC ATCACCCAGA TCCCAGAGGC GTTATGCCGG ACGGGCCACA 
TGCGATTGCC CGAATTGCCA GGAGGCGGAA CGCCTGGGTC CCGCTGGCGT TCATCTGCGC 
AAAAAGAACA TCCATTCGTG TCACATACCT GGCTGTGGCA AGGTGTATGG AAAGACGTCA 
CATCTGAAGG CTCACCTGCG GTGGCACACC GGCGAGCGTC CCTTTGTGTG CAACTGGCTG 
TTCTGCGGCA AGCGTTTCAC ACGCTCCGAC GAGCTGCAAC GGCATTTGAG GACGCACACC 
GGCGAGAAGC GCTTTGCATG CCCCGTGTGC AATAAGCGCT TTATGCGCAG CGACCATTTG 
GCCAAGCACG TGAAGACGCA CAATGGCACG GCCAATCACC AGGCCAATGG CCACAATGGT 
GGGCTGAAGA AGGGCTCCTC GGAGTCGTGT AGCGACTCGG AGGAGGCCGC CAACCAGTCG 
GGCGAGTCCA ACGGGCTGGG CGGCGTGGGC AGTGGCCCAC AGACGGGCGG GGCCGGTGGC 
GGCGGGGGTG GCTCTAGTTC GGGCAATGGT GCTGGTACGT TAGCGGTATC GGGATCGGTG 
ACCACCGGAG CGGGAGCTGG ATCCGGTACG GGTAGCTCCA ATTCCAATTC CAATTCCGGT 
GGCTCAAGCG GTTCCGTTAG CGGTTCCGTA TCCGGTTCTG GCAGCCATCC CGGCACCCCG 
ACCTCGCTGC ACGCGCACAG CGCCAATGGC ACGTCCAGCA GCCTGCTGGG CGGCGGTCTG 
CACCTGGCCA CGCCGCACCA GATGGTCGCC GCGGGCGGTT CGCCGGTGAT GCTTCACCAG 
CAGCAGCAGC AGCAGCAACA TCAGCAGCAA CAGCAGCAAC AGCAGCAGCA GCAGCATCAA 
CAGCAGCAAC AGCAGCAACA TCAACAGCAG CAGCAGCAAC ATCAGCAGCA GCAGCAGCAA 
CACCACCAGC AACAGCAGCA GCATCATCAC CAGCAATTTG GCCAGCAGCA GCATCCCCAT 
GCCCATCACC TGCACCACCA TGCCCACCAC GCGCACCACC TGGCCACCGG CGGCTCACCC 
GGTCTGGATC CCAGCTCCCT CGTGGATATA AAGCCACCGA TGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096927.2
CDS	917..3064
Translation	MLTDMTPTAGQLYGSQIPAMGMNISNIATHLQKPQVNPDHPSLRGTPLAMLAAQCNKLSNKSPPPLADAAVGKGFHPWKKSPNSPAAGSSGSSGGGGGGGGSSAGQHSPCAISAASSSSSSGSSGGQSSRSLSSSASTMVNITASRPLASSCAAVGGGSTGSSSSASGSQSSSTASAVAAAYGGDLYFPNTSTSNMDNHHMHQGLLGKVEAGAAAFGGVYSRHPYDWPFNAVTHKEAASVNSGWWDMHSAAGSWLDMGGAGMHSTMANYASENYSSALSHSLLGSGQHLLQDTYKSMLPGQGVGVGVGVGMGGFSLPHSSPSAAAAAAATAAAAAGGSPQGGSPSTPSPRSQRRYAGRATCDCPNCQEAERLGPAGVHLRKKNIHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLAKHVKTHNGTANHQANGHNGGLKKGSSESCSDSEEAANQSGESNGLGGVGSGPQTGGAGGGGGGSSSGNGAGTLAVSGSVTTGAGAGSGTGSSNSNSNSGGSSGSVSGSVSGSGSHPGTPTSLHAHSANGTSSSLLGGGLHLATPHQMVAAGGSPVMLHQQQQQQQHQQQQQQQQQQQHQQQQQQQHQQQQQQHQQQQQQHHQQQQQHHHQQFGQQQHPHAHHLHHHAHHAHHLATGGSPGLDPSSLVDIKPPMV

Target ORF information:

RefSeq Version	NM_001103457.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sp1, transcript variant D (Sp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103457.4

ORF Insert Sequence:

ATGTTAACGG ACATGACACC GACCGCCGGT CAGCTCTACG GATCCCAGAT TCCGGCCATG 
GGCATGAACA TCAGCAATAT CGCCACACAC TTGCAGAAGC CACAAGTTAA TCCGGATCAT 
CCCAGCTTGA GAGGAACTCC GCTGGCCATG CTGGCCGCCC AGTGCAACAA GCTATCCAAC 
AAATCCCCAC CCCCGCTGGC CGACGCCGCT GTGGGCAAGG GCTTCCATCC GTGGAAGAAG 
AGTCCCAATT CGCCGGCAGC CGGATCCAGT GGCTCCTCCG GCGGTGGTGG CGGCGGCGGT 
GGCAGTAGCG CCGGCCAGCA CTCGCCCTGC GCCATCTCAG CGGCCAGCTC GTCCAGCTCG 
AGCGGATCAA GCGGAGGACA GTCCTCCAGG AGCCTCTCAT CCAGCGCCAG CACCATGGTT 
AACATCACAG CTAGTCGCCC CCTGGCCTCC TCCTGTGCGG CGGTGGGCGG GGGCTCCACC 
GGCTCCTCCT CCTCCGCCTC TGGATCACAG TCATCCTCGA CGGCATCTGC GGTGGCAGCG 
GCCTACGGCG GTGATTTGTA CTTTCCGAAC ACATCCACCT CGAACATGGA CAACCATCAC 
ATGCATCAGG GTCTCCTGGG TAAAGTGGAG GCGGGTGCGG CTGCCTTTGG CGGTGTCTAT 
TCGCGTCATC CGTACGATTG GCCCTTCAAT GCGGTTACCC ACAAGGAGGC GGCCAGCGTG 
AATTCCGGCT GGTGGGACAT GCACAGTGCC GCCGGATCCT GGCTGGATAT GGGTGGTGCG 
GGCATGCACT CAACAATGGC CAATTATGCG AGTGAGAACT ATAGCTCCGC ATTGAGTCAT 
TCGCTGTTGG GATCGGGACA ACATTTGCTG CAGGACACCT ACAAGAGCAT GTTGCCCGGC 
CAGGGAGTTG GCGTTGGTGT GGGCGTTGGC ATGGGTGGCT TCTCCCTGCC TCACAGTTCA 
CCATCGGCGG CAGCAGCAGC TGCGGCCACA GCGGCAGCGG CGGCGGGTGG CTCCCCCCAG 
GGTGGTTCAC CATCGACACC ATCACCCAGA TCCCAGAGGC GTTATGCCGG ACGGGCCACA 
TGCGATTGCC CGAATTGCCA GGAGGCGGAA CGCCTGGGTC CCGCTGGCGT TCATCTGCGC 
AAAAAGAACA TCCATTCGTG TCACATACCT GGCTGTGGCA AGGTGTATGG AAAGACGTCA 
CATCTGAAGG CTCACCTGCG GTGGCACACC GGCGAGCGTC CCTTTGTGTG CAACTGGCTG 
TTCTGCGGCA AGCGTTTCAC ACGCTCCGAC GAGCTGCAAC GGCATTTGAG GACGCACACC 
GGCGAGAAGC GCTTTGCATG CCCCGTGTGC AATAAGCGCT TTATGCGCAG CGACCATTTG 
GCCAAGCACG TGAAGACGCA CAATGGCACG GCCAATCACC AGGCCAATGG CCACAATGGT 
GGGCTGAAGA AGGGCTCCTC GGAGTCGTGT AGCGACTCGG AGGAGGCCGC CAACCAGTCG 
GGCGAGTCCA ACGGGCTGGG CGGCGTGGGC AGTGGCCCAC AGACGGGCGG GGCCGGTGGC 
GGCGGGGGTG GCTCTAGTTC GGGCAATGGT GCTGGTACGT TAGCGGTATC GGGATCGGTG 
ACCACCGGAG CGGGAGCTGG ATCCGGTACG GGTAGCTCCA ATTCCAATTC CAATTCCGGT 
GGCTCAAGCG GTTCCGTTAG CGGTTCCGTA TCCGGTTCTG GCAGCCATCC CGGCACCCCG 
ACCTCGCTGC ACGCGCACAG CGCCAATGGC ACGTCCAGCA GCCTGCTGGG CGGCGGTCTG 
CACCTGGCCA CGCCGCACCA GATGGTCGCC GCGGGCGGTT CGCCGGTGAT GCTTCACCAG 
CAGCAGCAGC AGCAGCAACA TCAGCAGCAA CAGCAGCAAC AGCAGCAGCA GCAGCATCAA 
CAGCAGCAAC AGCAGCAACA TCAACAGCAG CAGCAGCAAC ATCAGCAGCA GCAGCAGCAA 
CACCACCAGC AACAGCAGCA GCATCATCAC CAGCAATTTG GCCAGCAGCA GCATCCCCAT 
GCCCATCACC TGCACCACCA TGCCCACCAC GCGCACCACC TGGCCACCGG CGGCTCACCC 
GGTCTGGATC CCAGCTCCCT CGTGGATATA AAGCCACCGA TGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sp1 ( NM_167200.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727360.1 Drosophila melanogaster Sp1, transcript variant B (Sp1), mRNA.

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CloneID	OFa03370
Clone ID Related Accession (Same CDS sequence)	NM_167200.4
Accession Version	NM_167200.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sp1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_167200.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAACC ATCACATGCA TCAGGGTCTC CTGGGTAAAG TGGAGGCGGG TGCGGCTGCC 
TTTGGCGGTG TCTATTCGCG TCATCCGTAC GATTGGCCCT TCAATGCGGT TACCCACAAG 
GAGGCGGCCA GCGTGAATTC CGGCTGGTGG GACATGCACA GTGCCGCCGG ATCCTGGCTG 
GATATGGGTG GTGCGGGCAT GCACTCAACA ATGGCCAATT ATGCGAGTGA GAACTATAGC 
TCCGCATTGA GTCATTCGCT GTTGGGATCG GGACAACATT TGCTGCAGGA CACCTACAAG 
AGCATGTTGC CCGGCCAGGG AGTTGGCGTT GGTGTGGGCG TTGGCATGGG TGGCTTCTCC 
CTGCCTCACA GTTCACCATC GGCGGCAGCA GCAGCTGCGG CCACAGCGGC AGCGGCGGCG 
GGTGGCTCCC CCCAGGGTGG TTCACCATCG ACACCATCAC CCAGATCCCA GAGGCGTTAT 
GCCGGACGGG CCACATGCGA TTGCCCGAAT TGCCAGGAGG CGGAACGCCT GGGTCCCGCT 
GGCGTTCATC TGCGCAAAAA GAACATCCAT TCGTGTCACA TACCTGGCTG TGGCAAGGTG 
TATGGAAAGA CGTCACATCT GAAGGCTCAC CTGCGGTGGC ACACCGGCGA GCGTCCCTTT 
GTGTGCAACT GGCTGTTCTG CGGCAAGCGT TTCACACGCT CCGACGAGCT GCAACGGCAT 
TTGAGGACGC ACACCGGCGA GAAGCGCTTT GCATGCCCCG TGTGCAATAA GCGCTTTATG 
CGCAGCGACC ATTTGGCCAA GCACGTGAAG ACGCACAATG GCACGGCCAA TCACCAGGCC 
AATGGCCACA ATGGTGGGCT GAAGAAGGGC TCCTCGGAGT CGTGTAGCGA CTCGGAGGAG 
GCCGCCAACC AGTCGGGCGA GTCCAACGGG CTGGGCGGCG TGGGCAGTGG CCCACAGACG 
GGCGGGGCCG GTGGCGGCGG GGGTGGCTCT AGTTCGGGCA ATGGTGCTGG TACGTTAGCG 
GTATCGGGAT CGGTGACCAC CGGAGCGGGA GCTGGATCCG GTACGGGTAG CTCCAATTCC 
AATTCCAATT CCGGTGGCTC AAGCGGTTCC GTTAGCGGTT CCGTATCCGG TTCTGGCAGC 
CATCCCGGCA CCCCGACCTC GCTGCACGCG CACAGCGCCA ATGGCACGTC CAGCAGCCTG 
CTGGGCGGCG GTCTGCACCT GGCCACGCCG CACCAGATGG TCGCCGCGGG CGGTTCGCCG 
GTGATGCTTC ACCAGCAGCA GCAGCAGCAG CAACATCAGC AGCAACAGCA GCAACAGCAG 
CAGCAGCAGC ATCAACAGCA GCAACAGCAG CAACATCAAC AGCAGCAGCA GCAACATCAG 
CAGCAGCAGC AGCAACACCA CCAGCAACAG CAGCAGCATC ATCACCAGCA ATTTGGCCAG 
CAGCAGCATC CCCATGCCCA TCACCTGCAC CACCATGCCC ACCACGCGCA CCACCTGGCC 
ACCGGCGGCT CACCCGGTCT GGATCCCAGC TCCCTCGTGG ATATAAAGCC ACCGATGGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727360.1
CDS	1308..2870
Translation	MDNHHMHQGLLGKVEAGAAAFGGVYSRHPYDWPFNAVTHKEAASVNSGWWDMHSAAGSWLDMGGAGMHSTMANYASENYSSALSHSLLGSGQHLLQDTYKSMLPGQGVGVGVGVGMGGFSLPHSSPSAAAAAAATAAAAAGGSPQGGSPSTPSPRSQRRYAGRATCDCPNCQEAERLGPAGVHLRKKNIHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLAKHVKTHNGTANHQANGHNGGLKKGSSESCSDSEEAANQSGESNGLGGVGSGPQTGGAGGGGGGSSSGNGAGTLAVSGSVTTGAGAGSGTGSSNSNSNSGGSSGSVSGSVSGSGSHPGTPTSLHAHSANGTSSSLLGGGLHLATPHQMVAAGGSPVMLHQQQQQQQHQQQQQQQQQQQHQQQQQQQHQQQQQQHQQQQQQHHQQQQQHHHQQFGQQQHPHAHHLHHHAHHAHHLATGGSPGLDPSSLVDIKPPMV

Target ORF information:

RefSeq Version	NM_167200.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sp1, transcript variant B (Sp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167200.4

ORF Insert Sequence:

ATGGACAACC ATCACATGCA TCAGGGTCTC CTGGGTAAAG TGGAGGCGGG TGCGGCTGCC 
TTTGGCGGTG TCTATTCGCG TCATCCGTAC GATTGGCCCT TCAATGCGGT TACCCACAAG 
GAGGCGGCCA GCGTGAATTC CGGCTGGTGG GACATGCACA GTGCCGCCGG ATCCTGGCTG 
GATATGGGTG GTGCGGGCAT GCACTCAACA ATGGCCAATT ATGCGAGTGA GAACTATAGC 
TCCGCATTGA GTCATTCGCT GTTGGGATCG GGACAACATT TGCTGCAGGA CACCTACAAG 
AGCATGTTGC CCGGCCAGGG AGTTGGCGTT GGTGTGGGCG TTGGCATGGG TGGCTTCTCC 
CTGCCTCACA GTTCACCATC GGCGGCAGCA GCAGCTGCGG CCACAGCGGC AGCGGCGGCG 
GGTGGCTCCC CCCAGGGTGG TTCACCATCG ACACCATCAC CCAGATCCCA GAGGCGTTAT 
GCCGGACGGG CCACATGCGA TTGCCCGAAT TGCCAGGAGG CGGAACGCCT GGGTCCCGCT 
GGCGTTCATC TGCGCAAAAA GAACATCCAT TCGTGTCACA TACCTGGCTG TGGCAAGGTG 
TATGGAAAGA CGTCACATCT GAAGGCTCAC CTGCGGTGGC ACACCGGCGA GCGTCCCTTT 
GTGTGCAACT GGCTGTTCTG CGGCAAGCGT TTCACACGCT CCGACGAGCT GCAACGGCAT 
TTGAGGACGC ACACCGGCGA GAAGCGCTTT GCATGCCCCG TGTGCAATAA GCGCTTTATG 
CGCAGCGACC ATTTGGCCAA GCACGTGAAG ACGCACAATG GCACGGCCAA TCACCAGGCC 
AATGGCCACA ATGGTGGGCT GAAGAAGGGC TCCTCGGAGT CGTGTAGCGA CTCGGAGGAG 
GCCGCCAACC AGTCGGGCGA GTCCAACGGG CTGGGCGGCG TGGGCAGTGG CCCACAGACG 
GGCGGGGCCG GTGGCGGCGG GGGTGGCTCT AGTTCGGGCA ATGGTGCTGG TACGTTAGCG 
GTATCGGGAT CGGTGACCAC CGGAGCGGGA GCTGGATCCG GTACGGGTAG CTCCAATTCC 
AATTCCAATT CCGGTGGCTC AAGCGGTTCC GTTAGCGGTT CCGTATCCGG TTCTGGCAGC 
CATCCCGGCA CCCCGACCTC GCTGCACGCG CACAGCGCCA ATGGCACGTC CAGCAGCCTG 
CTGGGCGGCG GTCTGCACCT GGCCACGCCG CACCAGATGG TCGCCGCGGG CGGTTCGCCG 
GTGATGCTTC ACCAGCAGCA GCAGCAGCAG CAACATCAGC AGCAACAGCA GCAACAGCAG 
CAGCAGCAGC ATCAACAGCA GCAACAGCAG CAACATCAAC AGCAGCAGCA GCAACATCAG 
CAGCAGCAGC AGCAACACCA CCAGCAACAG CAGCAGCATC ATCACCAGCA ATTTGGCCAG 
CAGCAGCATC CCCATGCCCA TCACCTGCAC CACCATGCCC ACCACGCGCA CCACCTGGCC 
ACCGGCGGCT CACCCGGTCT GGATCCCAGC TCCCTCGTGG ATATAAAGCC ACCGATGGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sp1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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