Ubr3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ubr3
Entrez Gene ID	31713
Full Name	Ubr3 ubiquitin ligase
Synonyms	CG1530,CG1531,CG42593,Dmel\CG42593,Dmel_CG1530,Dmel_CG1531,ubr3
General protein information	Preferred Names Ubr3 ubiquitin ligase Names CG42593 gene product from transcript CG42593-RA CG42593-PA CG42593-PB CG42593-PC Ubr3-PA Ubr3-PB Ubr3-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 7C8-7C9

mRNA and Protein(s)

mRNA	Protein	Name
NM_132200.4	NP_572428.3	Ubr3 ubiquitin ligase, isoform A
NM_001272388.1	NP_001259317.1	Ubr3 ubiquitin ligase, isoform B
NM_001272389.1	NP_001259318.1	Ubr3 ubiquitin ligase, isoform C

Pathways from BioSystems

Homologies

Bos taurus (cattle)	UBR3	XP_005202447.1
Mus musculus (house mouse)	Ubr3	NP_808451.2
Drosophila melanogaster (fruit fly)	CG42593	NP_001259318.1
	UBR3	XP_001099162.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000384	XP_310723.5
Homo sapiens (human)	UBR3	NP_742067.3
Pan troglodytes (chimpanzee)	UBR3	XP_003309461.1
Canis lupus familiaris (dog)	UBR3	XP_005640460.1
Rattus norvegicus (Norway rat)	Ubr3	XP_006234401.1
Gallus gallus (chicken)	ZNF650	XP_004942807.1
Danio rerio (zebrafish)	ubr3	XP_001919629.4
Xenopus tropicalis (tropical clawed frog)	ubr3	XP_002934668.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ubr3 negatively regulates the mono-ubiquitination of non-muscle Myosin II, a protein associated with hearing loss in humans.
Title: The E3 ligase Ubr3 regulates Usher syndrome and MYH9 disorder proteins in the auditory organs of Drosophila and mammals.

We identified Ubr3, an E3 ubiquitin ligase, as a novel, positive regulator of Hh signaling in Drosophila and vertebrates. Hh signaling regulates the Ubr3-mediated poly-ubiquitination and degradation of Cos2, a central component of Hh signaling. In developing Drosophila eye discs, loss of ubr3 leads to a delayed differentiation of photoreceptors and a reduction in Hh signaling.
Title: Ubr3, a Novel Modulator of Hh Signaling Affects the Degradation of Costal-2 and Kif7 through Poly-ubiquitination.

Ubr3 interacts with DIAP1 and positively regulates DIAP1 activity, possibly by maintaining its active conformation in the apoptosis pathway.
Title: Ubr3 E3 ligase regulates apoptosis by controlling the activity of DIAP1 in Drosophila.

The following Ubr3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ubr3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01058	NM_132200.4 Latest version!	Drosophila melanogaster Ubr3 ubiquitin ligase (Ubr3), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03020	NM_001272388.1 Latest version!	Drosophila melanogaster Ubr3 ubiquitin ligase (Ubr3), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03021	NM_001272389.1 Latest version!	Drosophila melanogaster Ubr3 ubiquitin ligase (Ubr3), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ubr3 ( NM_132200.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572428.3 Drosophila melanogaster Ubr3 ubiquitin ligase (Ubr3), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa01058
Clone ID Related Accession (Same CDS sequence)	NM_132200.4
Accession Version	NM_132200.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ubr3 ubiquitin ligase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_132200.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGAGG ATGACAACCT GTCCAATGCC GACATAAGCA TAGATGACGA GGAGGTGGTG 
CGGGAGCAGA CCCACCATCC GCCCATGCAA GAGGATCAGG AGCTGGATAA CGAAGATGGG 
TCATCCGACG TGGATCTGTC TTCGGTGTAC GTCGTGCCAC CCATAGTCTC GGCACCATCG 
CCCGCATCAG TGCGCATCGC TGGTGGATCC ACAATTGGTG GCGGAGGGGT CGCAGCTGGA 
GCAGTTCCAA CAACCACCGA TAACTCGCAT TCCCCCTTCC ACGATTGGGA TTCCAGTGTG 
GCGGCCGCTG CTGCTCCACC GCCGCCACCG CCCAGGACAG GACCAACTAC AACTACAAGC 
TCCGGCACAG CCGCGGAATC GGGAGCCGCG TCCGCCTTAT CCGACGATGC CGGCGCCAAG 
CCAAGTTTCT TCTTTGGCGC CTCCTCGTTC AGTTTGCGCA GCCGCAAGGA GGTGGCCGCC 
CTGATCAACA CGGAATGCTG CCGTGGCAGC CCCACACCCG ATCTCGACTC CATAATGGAT 
ACCCTATTCA ATCCGGGGAC GCCCATCGAC AATCTGGACA ACATCGAGTG GATACGATGG 
CTCATTGCCG GCGGACGGAC GCCGCAGGAG TTTGTTAAAA TTGTGCGCAG CTATGACAAC 
CACGCCAAAT GCGGTCTGGT ATGGGTACCG CATGTGGTGG CCTACAGGTG CCGCACTTGC 
GGCATATCGC CCTGCATGTC CATATGCCGG GACTGCTTCA AGAAGGGCAA CCACACGAAC 
CACGACTTCA ATATGTTCCT GTCACAGGCG GGCGGTGCCT GCGATTGTGG TGACACCTCG 
GTCATGAAGG CGGAGGGTTT TTGCAGTGAT CATGGCATCA ATAATCGGGT GAATCGCGAT 
CCGGTGCCAA ACAATCTTTT GGCAGTGGCC GAGGCCATAA TGCCCAAGCT GCTCTTCCGC 
CTGCTGCAGC ACTTCCGTGA GCACAGCGAC ACCCCACTGG AGGTGCAAGC GATAACGTCG 
TACTCCTGCG AGGAGTTCGC CAACATGCTG ATCGATCTGA ACAACATGGG CGAAATAATG 
CGCAAAGTGA TGACGCGCAC GCTGATAAAT CCGGAGGTGT ATGCCTTCTT CATGGAAGCA 
CCGTGCCAGG ACACCCGGAA TGGACGCTTC TTGAAGGCGA ACCGAGAGAA GTACGAGGAT 
GCCGTCAATA GATTCCCAAA CCCAGAGCCA CCCGATGAGT ACAGAGATCT GCCCGCGCTG 
GGCGACAAAT TGGTCCACAC CACGCTACTC GAAGAGTTCA TCTTCTGGAC ATTTAAGTTC 
GAGTTTCCAC AGACTCTGGT CTGCTTTCTG CTGAACATGC TGCCTGATCA GGATTACAAG 
GAACACCTCA CCCGCACATT TGTGATGCAC TACAGCCGCA TACCGTCCGT GTTGGAAATG 
TCCCGTGATC CGGACACGCT CAGCAACCGG GTGGTCCACA TGAGCGTCCA ACTCTTCTCT 
AACGAAAGTC TGGCCCTCAA GATGGTCAAC GAGCTGTCGC TGCTGCACGT AATGATTATT 
AGTCTGAAGC TAATGATGTC CAAGATACTC ATACAGAATA CATTGCATGA TCCCAACAAG 
AATTTTCATT TCGTCATCGA CTGCACGCGT CAGGTGATGA AGGACCACTG CTACTGGCCA 
CTAGTCTCGG ACTTTAACAA CGTCCTTTCA CACGAATCGG TGGCCCTGGT CTTTCTGCGG 
GACGACAATC TCATCGATAT GTGGTTCCAG TTTCTCCAGA TGCTCCAGGG CATGAATGTC 
AATGTGCGCG AGACGGCTTC TCATGTGGAA TTCGAGCCAA ATAGCTACTA TGCGGCGTTC 
TCCTGTGAGC TTGAGGCCAG TGCCTATCCC ATGTGGTCGA TTATCTCGCA TCTGCAGGAT 
GGCACACATG CCCATCTGGC AAAGAAGATC ATCAACTACT GTGTGACGAC GCTGCACGAG 
TGGCTAGACT CTATTTACTT TATGGAGGCG CGTCTATCTA TGGAGGAGAT GATGCAGGCT 
TCGTTCCACT TTCCCCTGCA TCGTTATCTG GCTGCCTTTG TTTGCCAGGC GGTAACTAAA 
ATGGGAATCA GTCTGAACGA TGTGCTGCCC TCACGCCCCT ATCTGCTGCC CCTGCTGATG 
ATCCATCCAC TTCGCGTCCA GAGTTTTTTC TACGAGATCC TGGCCGGAAA ATGGGTGCGC 
AATGGTCTCC AAATCAAGGG ACAAGCGATG ACCTATATCC AGGCGAACTT CTGCAACTCG 
ATGGCCGACA TGGACCTGTT CTTCCTGCAA ATCTGTGCCA CCAACCTGCC GCAGTACTTC 
TTTCTGCAGA ACACCATCGA GTTGTTCGAC GTAGGTCAAT GGCTGGAGAC AGCTCCCTTG 
AAGCAGCCTC AAAAGGCGGA GCAATCCTCG ATGCTGGAGG GATTCCTAAC CTTCTTGGCC 
ACCCTGGTGA CGAGTCGCAC TAATCTGGGA AACGATGAAG CCACCCAATG CATTATTGAG 
ATCAGTGCCT TGTTGGCTAC CGAGAACAAG ACGCATTCGC AGCTCCTTGA ACTAATGCCG 
GAGCGGTCGG GCAATGTGCA TACCAAAAAC TTTGAGACCT TCCTTAAGAA GCTATCGGTT 
TACAAGGCTC CGTCGAGTGG GAGCGAGAAC CTGGAGCAGG GTCTCTTCAC GCCCATCGAC 
GAGGTGTGGG AGAAGCACTA CGATCCGCTG CACGTCCTTT TGCGAGCAGT TCATCGTCGA 
GACTTTCAAT CATCGCTGGA CCGCTTTACA AACTATGTGA AGAGCAAGGA TAAGATGCCG 
GCAAGTGGTA ACCTATGGCC ACCCTTCCGA TTGCCTCATG CTCTGCCCGC AACGAGCTCC 
TTCAGTGATC CCTGCAAGAT TTTGAACTCG CGCATCTTTC ACTCCACCAT CCTGTCGATA 
TTTTTCCGTG CAGTTCACAC ACGTGATGTT TCCGAGCATC TGCTGGCTCT GGCTGTGTTC 
CTCTTAGAGA TTGCTGTGGA AACAAGTGAT GATGTTGGCA GTGGAACTGG AGATCGGGCC 
CCTCCTGCGC TGGCTGCTGT GGTGGAGTCA TCTAGCGGGC CGGGCTATCA TGGCTACGGC 
ACGGGACGGC ATGAGCCGCC CAAGCTTTTC CACTGCTATC CCACGGACAA TCTAAGCTGC 
AATCTGCGCC ACGTGGTCAA GAAAGTGTCG CTGAAGTCAC GCGATCCGCA AGTGATCACC 
TCCAGCTATC GGTCGAACCC ATTTTACTCA GATCTGGACT TTGAGGTTGA AGCGGATCCA 
GAGCAATCGA TGCGCATGAT TGGTCAAGGG GACCCCGAAG GAGATGAGGC AACGGGTGGA 
GCTGGACTGG GAATGGGAGC TCATAGCAGA AGGAATGTCT CTCAGGCGCT GGTGCCGATG 
AGAGTGCCCG GCATGGAGGT CGCCCTACCA CCGGATCTTT CAGTAGTTGC CGAAACCGGT 
GTGGTTATCC GCCAGGATAG CAACGAAGAT GATCTACTGA GAGAAGGCAA TCATGCCATG 
GATATGAGTC CACCGGCTGC TCTCGATTTC CACTTTCCGC TGCAGCAGAT CACTTTGCCG 
GAGAGCGGAA TGGAGGTGGC TATTCGGAGA GATCTTCTGC TAGCCGAAAC GAATAATATG 
GGCGCAATTG CAGGTGGAGC TGGAGGTGGC GCCAATGCAT CGGCCACACC ACCAGCAGGG 
GCTTCCAATG AGATGTTCTC GCCTACCACA CCCACAGGCA GCGGTATGCT CCTGCCTTTC 
CAGCGGGTTC AGCCTGTAGC CGTACCCAGT AGCGGTAATA TGGATATTGT ACCATCAAAT 
GCCATGGGTG CCGGCGGAAG TTTCACATCC GGCGTAAGTG GTAGCGGCAC TGGACGTAGA 
ATGAACTACG AAACGGGTGG AGCACGTAAG CGATCCGTGG ACATTGCCAT CGGTGGCAGC 
AATAAGGATG AGCTGCATTT GGACGAAAGC ATTTTGTCCC TGCTACTAAA GCTTCATTCT 
CAGCTCTCAG GCACACTAGA TAGCTTCTCG TTGAGCGACG GTGAAGATCA ATCATCTGAC 
GACGATTCGA CCATGGATGT GGACTGTAAC GAAGCCAGCA CCTCCATGGC AGCAGCGGAG 
AGCACTGCCC TGGCTGAGAG AGGACGCAGT AAGCGCAACT ACAAGAACAT TCATGTATCC 
TCCTCGCGCA TCGGTGACGG ACCATTCTTT ATTGGTAATC TGCTGCGAAA GATCGCCAAG 
CAGGATGAGC AGTGCGCCCA GAGCATCGAC GACATCCGTG CCAGACTCTG GCCGAATCAG 
CGTGAAAAAC AAGCAGAGGC GAAAGCACGC GAGGCCAAGG AGAAGGAGGA GCGCCGCAAA 
AAAGCACGTG AGCGCCAGCA GAAGATGATG CAAGACTTCG CCAACAAGCA GAAGCTCTTC 
ATGCAATCCG CTGCAGCGTC ATCCAGTGGC ATGGGCTACG GACCGGAGGA CGAGGACGAT 
GAGGAGTTGT ACGAGGAGCA GCCGCGTGAG AAAGAGTACG ATTGCATAAT CTGCAATTGC 
ACTACGCCCA GTACCGAATC GAATCCTATC GGCTTGGTCG TTCTGGTCGA GTCCAGCGGC 
ATTGTTGGTC ATCGGCGGCG CATCGCCGAG CGATTGCCGC TGCCGATAAA CGCGGAGGAT 
GAGTCCCGAC TGGCGCACAC CACTCGACTG GCGGCTGAAT TCAACAGGCG CACGGAACTG 
CTCAGTCTGA AGTTTGGCGA TGAGTCCTGG TATCTGTCCA ATAATATGGC CTACGACAAC 
GGTGTACATG TGCAGTCGTG TGGCCACCAC GTTCATCTCA GTTGCCTAGA GGCATACCTT 
AAGACGCTGT ACACCACCCA ACGTCAGCCA GTTCAGGATC GTGGCGAGTT CTACTGTCCC 
GTGTGCCGAC AGCTCTCAAA CTCGGTGCTG CCGCTGAGTC CACAACTGGA CAGACCCACG 
CACCTGGTCC GCTCTGGTAA TCAGCCATTC GAACGTCTGG TCGCCGATCT AACAGATCTG 
ATTAAAGAGA ACGAAACGAT ACCGCAGCCA ACAAAACTGA CAGAGGCCAT GGGCCATGCC 
ATGGAGGTGA TGACAAACAT TGCCCAGCGC AAGGTTAAAT GCTCCTCGAT AACGTTCCGC 
AAGCTGTTCA TTTTCGTTAC CTCGATTGCG CGTACAAACC TGGAGGCGGA GATCATCCAG 
CGGGGCGGAT CACTATGTAC AGCGAATGCC ACGCGGTACA AGCCAAAGCG GGAATGCATC 
GTACCGCTAC TGCACGTGCT ATCGGTACAT GTGCGTGTCT TGGTTGAATG GCCGCTGTGG 
AGCAGCTGGG CCTCGTTAGC TGGCCTGCCC GTTACCGCTA CGGAACCGTT GCCGGCGCAC 
TGCCTCGAAT TGATTCCCAG CATATTAGCG GATCCCATTG CTCTGCTGCT CAAGTTTATC 
CTGCTGGCGC CACTGCAACT CGATCAGGAT TACTTTACGT GCATGGTGAA AGTCATGTAC 
AATCTGCTGT ACTATCAAAT TGTGGTTCAG CTGTGCGTTA CTCTCACCGA TTTAGAGTGC 
GATCACATTG TCAAAGTTTA CGGCAGCACT AGCGTTGGCA GTGACAACTC TGCTGCGGAA 
TCCCAACAGC AGGAGTCTGC CGCCGGGACA ACGAACAACC GTCGCCGTGC TGGACAACAG 
CAGCAGAGCT CCTCGCAGCT AGGAAAGGCC ATGGCCCTGG TGCTGAGCCA GACAAATGAC 
CTAGTTCACT TGCGACGCGA CTGCATACCC AGTACCAGCA GCTCGGCGGC AGCGTCGGCG 
GCTGGTTCCT CCTCCACAAC GTCCACAAAC CATGGAGCTA GCGCAGCCAC AGCCTCCTCT 
GCCACGACGA TCGAGGTTAA TCTGAAGTCC ATGGAATTAC AGCTGCAGGC GCTATGCTTG 
CCGTTTTTGC GAGTAGCTGC CCTGCTGCGC CAGCATCTCT ATCGCCATGA GATGCCGGAG 
ATCTCGGCAC CGGGACTGGA ATTTGTGCGC CTTGTTTACT ATTTGGAACT CGTAACAGAT 
TCTATGGACT GGGATTGCTT TAATGCCAGC AAGGGGCTAT GCTTTATTCC CGGCACTGAG 
ACAACGCTGC CCCAGTTCTG GTGCCAGCAG TTGATGGAGG TGCGTCCGCC GGCCGATACG 
GTCAGAGAAT TGGTGCTCAT CAATCAGCAT TCGTTGTGGC AGCAGCCGCG ACTTCTTGAA 
TTGCCGCGCG AGTACGAACG CTTGTTCACG TACTACCATG AGCGGCCGTG CCTCAATTGC 
TATAAAGTGC CCAAGGAGAG CTCCATCTGC CTGCTGTGCG GGACGATCGT ATGCCTCAAG 
CAGAACTGCT GCGCGGAGAA TGACTGCTGC GAGGCTGTTC GCCATACGCT ATCCTGTGGC 
GGTGGCATAG GCATCTTTTT GGTGGTCACC TCCACGTACA TCATTGTTAT CCGCGGACGA 
CGCGCCTGCC TTTGGGGCTC ACTCTATCTG GACGACTTCG ATGAGGAGGA TCGCGATCTC 
AAGCGCGGCA AACCTTTGTA TTTGAGCAAA GACCGCTTCA ATCTCCTGGA GTCGCAGTGG 
CTGTCCCATA AGTTTGCTCA TACAAAACAC ACATGGGTGT TTCACCGGGA TCTCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572428.3
CDS	1074..7733
Translation	MDEDDNLSNADISIDDEEVVREQTHHPPMQEDQELDNEDGSSDVDLSSVYVVPPIVSAPSPASVRIAGGSTIGGGGVAAGAVPTTTDNSHSPFHDWDSSVAAAAAPPPPPPRTGPTTTTSSGTAAESGAASALSDDAGAKPSFFFGASSFSLRSRKEVAALINTECCRGSPTPDLDSIMDTLFNPGTPIDNLDNIEWIRWLIAGGRTPQEFVKIVRSYDNHAKCGLVWVPHVVAYRCRTCGISPCMSICRDCFKKGNHTNHDFNMFLSQAGGACDCGDTSVMKAEGFCSDHGINNRVNRDPVPNNLLAVAEAIMPKLLFRLLQHFREHSDTPLEVQAITSYSCEEFANMLIDLNNMGEIMRKVMTRTLINPEVYAFFMEAPCQDTRNGRFLKANREKYEDAVNRFPNPEPPDEYRDLPALGDKLVHTTLLEEFIFWTFKFEFPQTLVCFLLNMLPDQDYKEHLTRTFVMHYSRIPSVLEMSRDPDTLSNRVVHMSVQLFSNESLALKMVNELSLLHVMIISLKLMMSKILIQNTLHDPNKNFHFVIDCTRQVMKDHCYWPLVSDFNNVLSHESVALVFLRDDNLIDMWFQFLQMLQGMNVNVRETASHVEFEPNSYYAAFSCELEASAYPMWSIISHLQDGTHAHLAKKIINYCVTTLHEWLDSIYFMEARLSMEEMMQASFHFPLHRYLAAFVCQAVTKMGISLNDVLPSRPYLLPLLMIHPLRVQSFFYEILAGKWVRNGLQIKGQAMTYIQANFCNSMADMDLFFLQICATNLPQYFFLQNTIELFDVGQWLETAPLKQPQKAEQSSMLEGFLTFLATLVTSRTNLGNDEATQCIIEISALLATENKTHSQLLELMPERSGNVHTKNFETFLKKLSVYKAPSSGSENLEQGLFTPIDEVWEKHYDPLHVLLRAVHRRDFQSSLDRFTNYVKSKDKMPASGNLWPPFRLPHALPATSSFSDPCKILNSRIFHSTILSIFFRAVHTRDVSEHLLALAVFLLEIAVETSDDVGSGTGDRAPPALAAVVESSSGPGYHGYGTGRHEPPKLFHCYPTDNLSCNLRHVVKKVSLKSRDPQVITSSYRSNPFYSDLDFEVEADPEQSMRMIGQGDPEGDEATGGAGLGMGAHSRRNVSQALVPMRVPGMEVALPPDLSVVAETGVVIRQDSNEDDLLREGNHAMDMSPPAALDFHFPLQQITLPESGMEVAIRRDLLLAETNNMGAIAGGAGGGANASATPPAGASNEMFSPTTPTGSGMLLPFQRVQPVAVPSSGNMDIVPSNAMGAGGSFTSGVSGSGTGRRMNYETGGARKRSVDIAIGGSNKDELHLDESILSLLLKLHSQLSGTLDSFSLSDGEDQSSDDDSTMDVDCNEASTSMAAAESTALAERGRSKRNYKNIHVSSSRIGDGPFFIGNLLRKIAKQDEQCAQSIDDIRARLWPNQREKQAEAKAREAKEKEERRKKARERQQKMMQDFANKQKLFMQSAAASSSGMGYGPEDEDDEELYEEQPREKEYDCIICNCTTPSTESNPIGLVVLVESSGIVGHRRRIAERLPLPINAEDESRLAHTTRLAAEFNRRTELLSLKFGDESWYLSNNMAYDNGVHVQSCGHHVHLSCLEAYLKTLYTTQRQPVQDRGEFYCPVCRQLSNSVLPLSPQLDRPTHLVRSGNQPFERLVADLTDLIKENETIPQPTKLTEAMGHAMEVMTNIAQRKVKCSSITFRKLFIFVTSIARTNLEAEIIQRGGSLCTANATRYKPKRECIVPLLHVLSVHVRVLVEWPLWSSWASLAGLPVTATEPLPAHCLELIPSILADPIALLLKFILLAPLQLDQDYFTCMVKVMYNLLYYQIVVQLCVTLTDLECDHIVKVYGSTSVGSDNSAAESQQQESAAGTTNNRRRAGQQQQSSSQLGKAMALVLSQTNDLVHLRRDCIPSTSSSAAASAAGSSSTTSTNHGASAATASSATTIEVNLKSMELQLQALCLPFLRVAALLRQHLYRHEMPEISAPGLEFVRLVYYLELVTDSMDWDCFNASKGLCFIPGTETTLPQFWCQQLMEVRPPADTVRELVLINQHSLWQQPRLLELPREYERLFTYYHERPCLNCYKVPKESSICLLCGTIVCLKQNCCAENDCCEAVRHTLSCGGGIGIFLVVTSTYIIVIRGRRACLWGSLYLDDFDEEDRDLKRGKPLYLSKDRFNLLESQWLSHKFAHTKHTWVFHRDLL

Target ORF information:

RefSeq Version	NM_132200.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ubr3 ubiquitin ligase (Ubr3), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132200.4

ORF Insert Sequence:

ATGGACGAGG ATGACAACCT GTCCAATGCC GACATAAGCA TAGATGACGA GGAGGTGGTG 
CGGGAGCAGA CCCACCATCC GCCCATGCAA GAGGATCAGG AGCTGGATAA CGAAGATGGG 
TCATCCGACG TGGATCTGTC TTCGGTGTAC GTCGTGCCAC CCATAGTCTC GGCACCATCG 
CCCGCATCAG TGCGCATCGC TGGTGGATCC ACAATTGGTG GCGGAGGGGT CGCAGCTGGA 
GCAGTTCCAA CAACCACCGA TAACTCGCAT TCCCCCTTCC ACGATTGGGA TTCCAGTGTG 
GCGGCCGCTG CTGCTCCACC GCCGCCACCG CCCAGGACAG GACCAACTAC AACTACAAGC 
TCCGGCACAG CCGCGGAATC GGGAGCCGCG TCCGCCTTAT CCGACGATGC CGGCGCCAAG 
CCAAGTTTCT TCTTTGGCGC CTCCTCGTTC AGTTTGCGCA GCCGCAAGGA GGTGGCCGCC 
CTGATCAACA CGGAATGCTG CCGTGGCAGC CCCACACCCG ATCTCGACTC CATAATGGAT 
ACCCTATTCA ATCCGGGGAC GCCCATCGAC AATCTGGACA ACATCGAGTG GATACGATGG 
CTCATTGCCG GCGGACGGAC GCCGCAGGAG TTTGTTAAAA TTGTGCGCAG CTATGACAAC 
CACGCCAAAT GCGGTCTGGT ATGGGTACCG CATGTGGTGG CCTACAGGTG CCGCACTTGC 
GGCATATCGC CCTGCATGTC CATATGCCGG GACTGCTTCA AGAAGGGCAA CCACACGAAC 
CACGACTTCA ATATGTTCCT GTCACAGGCG GGCGGTGCCT GCGATTGTGG TGACACCTCG 
GTCATGAAGG CGGAGGGTTT TTGCAGTGAT CATGGCATCA ATAATCGGGT GAATCGCGAT 
CCGGTGCCAA ACAATCTTTT GGCAGTGGCC GAGGCCATAA TGCCCAAGCT GCTCTTCCGC 
CTGCTGCAGC ACTTCCGTGA GCACAGCGAC ACCCCACTGG AGGTGCAAGC GATAACGTCG 
TACTCCTGCG AGGAGTTCGC CAACATGCTG ATCGATCTGA ACAACATGGG CGAAATAATG 
CGCAAAGTGA TGACGCGCAC GCTGATAAAT CCGGAGGTGT ATGCCTTCTT CATGGAAGCA 
CCGTGCCAGG ACACCCGGAA TGGACGCTTC TTGAAGGCGA ACCGAGAGAA GTACGAGGAT 
GCCGTCAATA GATTCCCAAA CCCAGAGCCA CCCGATGAGT ACAGAGATCT GCCCGCGCTG 
GGCGACAAAT TGGTCCACAC CACGCTACTC GAAGAGTTCA TCTTCTGGAC ATTTAAGTTC 
GAGTTTCCAC AGACTCTGGT CTGCTTTCTG CTGAACATGC TGCCTGATCA GGATTACAAG 
GAACACCTCA CCCGCACATT TGTGATGCAC TACAGCCGCA TACCGTCCGT GTTGGAAATG 
TCCCGTGATC CGGACACGCT CAGCAACCGG GTGGTCCACA TGAGCGTCCA ACTCTTCTCT 
AACGAAAGTC TGGCCCTCAA GATGGTCAAC GAGCTGTCGC TGCTGCACGT AATGATTATT 
AGTCTGAAGC TAATGATGTC CAAGATACTC ATACAGAATA CATTGCATGA TCCCAACAAG 
AATTTTCATT TCGTCATCGA CTGCACGCGT CAGGTGATGA AGGACCACTG CTACTGGCCA 
CTAGTCTCGG ACTTTAACAA CGTCCTTTCA CACGAATCGG TGGCCCTGGT CTTTCTGCGG 
GACGACAATC TCATCGATAT GTGGTTCCAG TTTCTCCAGA TGCTCCAGGG CATGAATGTC 
AATGTGCGCG AGACGGCTTC TCATGTGGAA TTCGAGCCAA ATAGCTACTA TGCGGCGTTC 
TCCTGTGAGC TTGAGGCCAG TGCCTATCCC ATGTGGTCGA TTATCTCGCA TCTGCAGGAT 
GGCACACATG CCCATCTGGC AAAGAAGATC ATCAACTACT GTGTGACGAC GCTGCACGAG 
TGGCTAGACT CTATTTACTT TATGGAGGCG CGTCTATCTA TGGAGGAGAT GATGCAGGCT 
TCGTTCCACT TTCCCCTGCA TCGTTATCTG GCTGCCTTTG TTTGCCAGGC GGTAACTAAA 
ATGGGAATCA GTCTGAACGA TGTGCTGCCC TCACGCCCCT ATCTGCTGCC CCTGCTGATG 
ATCCATCCAC TTCGCGTCCA GAGTTTTTTC TACGAGATCC TGGCCGGAAA ATGGGTGCGC 
AATGGTCTCC AAATCAAGGG ACAAGCGATG ACCTATATCC AGGCGAACTT CTGCAACTCG 
ATGGCCGACA TGGACCTGTT CTTCCTGCAA ATCTGTGCCA CCAACCTGCC GCAGTACTTC 
TTTCTGCAGA ACACCATCGA GTTGTTCGAC GTAGGTCAAT GGCTGGAGAC AGCTCCCTTG 
AAGCAGCCTC AAAAGGCGGA GCAATCCTCG ATGCTGGAGG GATTCCTAAC CTTCTTGGCC 
ACCCTGGTGA CGAGTCGCAC TAATCTGGGA AACGATGAAG CCACCCAATG CATTATTGAG 
ATCAGTGCCT TGTTGGCTAC CGAGAACAAG ACGCATTCGC AGCTCCTTGA ACTAATGCCG 
GAGCGGTCGG GCAATGTGCA TACCAAAAAC TTTGAGACCT TCCTTAAGAA GCTATCGGTT 
TACAAGGCTC CGTCGAGTGG GAGCGAGAAC CTGGAGCAGG GTCTCTTCAC GCCCATCGAC 
GAGGTGTGGG AGAAGCACTA CGATCCGCTG CACGTCCTTT TGCGAGCAGT TCATCGTCGA 
GACTTTCAAT CATCGCTGGA CCGCTTTACA AACTATGTGA AGAGCAAGGA TAAGATGCCG 
GCAAGTGGTA ACCTATGGCC ACCCTTCCGA TTGCCTCATG CTCTGCCCGC AACGAGCTCC 
TTCAGTGATC CCTGCAAGAT TTTGAACTCG CGCATCTTTC ACTCCACCAT CCTGTCGATA 
TTTTTCCGTG CAGTTCACAC ACGTGATGTT TCCGAGCATC TGCTGGCTCT GGCTGTGTTC 
CTCTTAGAGA TTGCTGTGGA AACAAGTGAT GATGTTGGCA GTGGAACTGG AGATCGGGCC 
CCTCCTGCGC TGGCTGCTGT GGTGGAGTCA TCTAGCGGGC CGGGCTATCA TGGCTACGGC 
ACGGGACGGC ATGAGCCGCC CAAGCTTTTC CACTGCTATC CCACGGACAA TCTAAGCTGC 
AATCTGCGCC ACGTGGTCAA GAAAGTGTCG CTGAAGTCAC GCGATCCGCA AGTGATCACC 
TCCAGCTATC GGTCGAACCC ATTTTACTCA GATCTGGACT TTGAGGTTGA AGCGGATCCA 
GAGCAATCGA TGCGCATGAT TGGTCAAGGG GACCCCGAAG GAGATGAGGC AACGGGTGGA 
GCTGGACTGG GAATGGGAGC TCATAGCAGA AGGAATGTCT CTCAGGCGCT GGTGCCGATG 
AGAGTGCCCG GCATGGAGGT CGCCCTACCA CCGGATCTTT CAGTAGTTGC CGAAACCGGT 
GTGGTTATCC GCCAGGATAG CAACGAAGAT GATCTACTGA GAGAAGGCAA TCATGCCATG 
GATATGAGTC CACCGGCTGC TCTCGATTTC CACTTTCCGC TGCAGCAGAT CACTTTGCCG 
GAGAGCGGAA TGGAGGTGGC TATTCGGAGA GATCTTCTGC TAGCCGAAAC GAATAATATG 
GGCGCAATTG CAGGTGGAGC TGGAGGTGGC GCCAATGCAT CGGCCACACC ACCAGCAGGG 
GCTTCCAATG AGATGTTCTC GCCTACCACA CCCACAGGCA GCGGTATGCT CCTGCCTTTC 
CAGCGGGTTC AGCCTGTAGC CGTACCCAGT AGCGGTAATA TGGATATTGT ACCATCAAAT 
GCCATGGGTG CCGGCGGAAG TTTCACATCC GGCGTAAGTG GTAGCGGCAC TGGACGTAGA 
ATGAACTACG AAACGGGTGG AGCACGTAAG CGATCCGTGG ACATTGCCAT CGGTGGCAGC 
AATAAGGATG AGCTGCATTT GGACGAAAGC ATTTTGTCCC TGCTACTAAA GCTTCATTCT 
CAGCTCTCAG GCACACTAGA TAGCTTCTCG TTGAGCGACG GTGAAGATCA ATCATCTGAC 
GACGATTCGA CCATGGATGT GGACTGTAAC GAAGCCAGCA CCTCCATGGC AGCAGCGGAG 
AGCACTGCCC TGGCTGAGAG AGGACGCAGT AAGCGCAACT ACAAGAACAT TCATGTATCC 
TCCTCGCGCA TCGGTGACGG ACCATTCTTT ATTGGTAATC TGCTGCGAAA GATCGCCAAG 
CAGGATGAGC AGTGCGCCCA GAGCATCGAC GACATCCGTG CCAGACTCTG GCCGAATCAG 
CGTGAAAAAC AAGCAGAGGC GAAAGCACGC GAGGCCAAGG AGAAGGAGGA GCGCCGCAAA 
AAAGCACGTG AGCGCCAGCA GAAGATGATG CAAGACTTCG CCAACAAGCA GAAGCTCTTC 
ATGCAATCCG CTGCAGCGTC ATCCAGTGGC ATGGGCTACG GACCGGAGGA CGAGGACGAT 
GAGGAGTTGT ACGAGGAGCA GCCGCGTGAG AAAGAGTACG ATTGCATAAT CTGCAATTGC 
ACTACGCCCA GTACCGAATC GAATCCTATC GGCTTGGTCG TTCTGGTCGA GTCCAGCGGC 
ATTGTTGGTC ATCGGCGGCG CATCGCCGAG CGATTGCCGC TGCCGATAAA CGCGGAGGAT 
GAGTCCCGAC TGGCGCACAC CACTCGACTG GCGGCTGAAT TCAACAGGCG CACGGAACTG 
CTCAGTCTGA AGTTTGGCGA TGAGTCCTGG TATCTGTCCA ATAATATGGC CTACGACAAC 
GGTGTACATG TGCAGTCGTG TGGCCACCAC GTTCATCTCA GTTGCCTAGA GGCATACCTT 
AAGACGCTGT ACACCACCCA ACGTCAGCCA GTTCAGGATC GTGGCGAGTT CTACTGTCCC 
GTGTGCCGAC AGCTCTCAAA CTCGGTGCTG CCGCTGAGTC CACAACTGGA CAGACCCACG 
CACCTGGTCC GCTCTGGTAA TCAGCCATTC GAACGTCTGG TCGCCGATCT AACAGATCTG 
ATTAAAGAGA ACGAAACGAT ACCGCAGCCA ACAAAACTGA CAGAGGCCAT GGGCCATGCC 
ATGGAGGTGA TGACAAACAT TGCCCAGCGC AAGGTTAAAT GCTCCTCGAT AACGTTCCGC 
AAGCTGTTCA TTTTCGTTAC CTCGATTGCG CGTACAAACC TGGAGGCGGA GATCATCCAG 
CGGGGCGGAT CACTATGTAC AGCGAATGCC ACGCGGTACA AGCCAAAGCG GGAATGCATC 
GTACCGCTAC TGCACGTGCT ATCGGTACAT GTGCGTGTCT TGGTTGAATG GCCGCTGTGG 
AGCAGCTGGG CCTCGTTAGC TGGCCTGCCC GTTACCGCTA CGGAACCGTT GCCGGCGCAC 
TGCCTCGAAT TGATTCCCAG CATATTAGCG GATCCCATTG CTCTGCTGCT CAAGTTTATC 
CTGCTGGCGC CACTGCAACT CGATCAGGAT TACTTTACGT GCATGGTGAA AGTCATGTAC 
AATCTGCTGT ACTATCAAAT TGTGGTTCAG CTGTGCGTTA CTCTCACCGA TTTAGAGTGC 
GATCACATTG TCAAAGTTTA CGGCAGCACT AGCGTTGGCA GTGACAACTC TGCTGCGGAA 
TCCCAACAGC AGGAGTCTGC CGCCGGGACA ACGAACAACC GTCGCCGTGC TGGACAACAG 
CAGCAGAGCT CCTCGCAGCT AGGAAAGGCC ATGGCCCTGG TGCTGAGCCA GACAAATGAC 
CTAGTTCACT TGCGACGCGA CTGCATACCC AGTACCAGCA GCTCGGCGGC AGCGTCGGCG 
GCTGGTTCCT CCTCCACAAC GTCCACAAAC CATGGAGCTA GCGCAGCCAC AGCCTCCTCT 
GCCACGACGA TCGAGGTTAA TCTGAAGTCC ATGGAATTAC AGCTGCAGGC GCTATGCTTG 
CCGTTTTTGC GAGTAGCTGC CCTGCTGCGC CAGCATCTCT ATCGCCATGA GATGCCGGAG 
ATCTCGGCAC CGGGACTGGA ATTTGTGCGC CTTGTTTACT ATTTGGAACT CGTAACAGAT 
TCTATGGACT GGGATTGCTT TAATGCCAGC AAGGGGCTAT GCTTTATTCC CGGCACTGAG 
ACAACGCTGC CCCAGTTCTG GTGCCAGCAG TTGATGGAGG TGCGTCCGCC GGCCGATACG 
GTCAGAGAAT TGGTGCTCAT CAATCAGCAT TCGTTGTGGC AGCAGCCGCG ACTTCTTGAA 
TTGCCGCGCG AGTACGAACG CTTGTTCACG TACTACCATG AGCGGCCGTG CCTCAATTGC 
TATAAAGTGC CCAAGGAGAG CTCCATCTGC CTGCTGTGCG GGACGATCGT ATGCCTCAAG 
CAGAACTGCT GCGCGGAGAA TGACTGCTGC GAGGCTGTTC GCCATACGCT ATCCTGTGGC 
GGTGGCATAG GCATCTTTTT GGTGGTCACC TCCACGTACA TCATTGTTAT CCGCGGACGA 
CGCGCCTGCC TTTGGGGCTC ACTCTATCTG GACGACTTCG ATGAGGAGGA TCGCGATCTC 
AAGCGCGGCA AACCTTTGTA TTTGAGCAAA GACCGCTTCA ATCTCCTGGA GTCGCAGTGG 
CTGTCCCATA AGTTTGCTCA TACAAAACAC ACATGGGTGT TTCACCGGGA TCTCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ubr3 ( NM_001272388.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259317.1 Drosophila melanogaster Ubr3 ubiquitin ligase (Ubr3), transcript variant B, mRNA.

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CloneID	OFa03020
Clone ID Related Accession (Same CDS sequence)	NM_001272388.1
Accession Version	NM_001272388.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ubr3 ubiquitin ligase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGAGG ATGACAACCT GTCCAATGCC GACATAAGCA TAGATGACGA GGAGGTGGTG 
CGGGAGCAGA CCCACCATCC GCCCATGCAA GAGGATCAGG AGCTGGATAA CGAAGATGGG 
TCATCCGACG TGGATCTGTC TTCGGTGTAC GTCGTGCCAC CCATAGTCTC GGCACCATCG 
CCCGCATCAG TGCGCATCGC TGGTGGATCC ACAATTGGTG GCGGAGGGGT CGCAGCTGGA 
GCAGTTCCAA CAACCACCGA TAACTCGCAT TCCCCCTTCC ACGATTGGGA TTCCAGTGTG 
GCGGCCGCTG CTGCTCCACC GCCGCCACCG CCCAGGACAG GACCAACTAC AACTACAAGC 
TCCGGCACAG CCGCGGAATC GGGAGCCGCG TCCGCCTTAT CCGACGATGC CGGCGCCAAG 
CCAAGTTTCT TCTTTGGCGC CTCCTCGTTC AGTTTGCGCA GCCGCAAGGA GGTGGCCGCC 
CTGATCAACA CGGAATGCTG CCGTGGCAGC CCCACACCCG ATCTCGACTC CATAATGGAT 
ACCCTATTCA ATCCGGGGAC GCCCATCGAC AATCTGGACA ACATCGAGTG GATACGATGG 
CTCATTGCCG GCGGACGGAC GCCGCAGGAG TTTGTTAAAA TTGTGCGCAG CTATGACAAC 
CACGCCAAAT GCGGTCTGGT ATGGGTACCG CATGTGGTGG CCTACAGGTG CCGCACTTGC 
GGCATATCGC CCTGCATGTC CATATGCCGG GACTGCTTCA AGAAGGGCAA CCACACGAAC 
CACGACTTCA ATATGTTCCT GTCACAGGCG GGCGGTGCCT GCGATTGTGG TGACACCTCG 
GTCATGAAGG CGGAGGGTTT TTGCAGTGAT CATGGCATCA ATAATCGGGT GAATCGCGAT 
CCGGTGCCAA ACAATCTTTT GGCAGTGGCC GAGGCCATAA TGCCCAAGCT GCTCTTCCGC 
CTGCTGCAGC ACTTCCGTGA GCACAGCGAC ACCCCACTGG AGGTGCAAGC GATAACGTCG 
TACTCCTGCG AGGAGTTCGC CAACATGCTG ATCGATCTGA ACAACATGGG CGAAATAATG 
CGCAAAGTGA TGACGCGCAC GCTGATAAAT CCGGAGGTGT ATGCCTTCTT CATGGAAGCA 
CCGTGCCAGG ACACCCGGAA TGGACGCTTC TTGAAGGCGA ACCGAGAGAA GTACGAGGAT 
GCCGTCAATA GATTCCCAAA CCCAGAGCCA CCCGATGAGT ACAGAGATCT GCCCGCGCTG 
GGCGACAAAT TGGTCCACAC CACGCTACTC GAAGAGTTCA TCTTCTGGAC ATTTAAGTTC 
GAGTTTCCAC AGACTCTGGT CTGCTTTCTG CTGAACATGC TGCCTGATCA GGATTACAAG 
GAACACCTCA CCCGCACATT TGTGATGCAC TACAGCCGCA TACCGTCCGT GTTGGAAATG 
TCCCGTGATC CGGACACGCT CAGCAACCGG GTGGTCCACA TGAGCGTCCA ACTCTTCTCT 
AACGAAAGTC TGGCCCTCAA GATGGTCAAC GAGCTGTCGC TGCTGCACGT AATGATTATT 
AGTCTGAAGC TAATGATGTC CAAGATACTC ATACAGAATA CATTGCATGA TCCCAACAAG 
AATTTTCATT TCGTCATCGA CTGCACGCGT CAGGTGATGA AGGACCACTG CTACTGGCCA 
CTAGTCTCGG ACTTTAACAA CGTCCTTTCA CACGAATCGG TGGCCCTGGT CTTTCTGCGG 
GACGACAATC TCATCGATAT GTGGTTCCAG TTTCTCCAGA TGCTCCAGGG CATGAATGTC 
AATGTGCGCG AGACGGCTTC TCATGTGGAA TTCGAGCCAA ATAGCTACTA TGCGGCGTTC 
TCCTGTGAGC TTGAGGCCAG TGCCTATCCC ATGTGGTCGA TTATCTCGCA TCTGCAGGAT 
GGCACACATG CCCATCTGGC AAAGAAGATC ATCAACTACT GTGTGACGAC GCTGCACGAG 
TGGCTAGACT CTATTTACTT TATGGAGGCG CGTCTATCTA TGGAGGAGAT GATGCAGGCT 
TCGTTCCACT TTCCCCTGCA TCGTTATCTG GCTGCCTTTG TTTGCCAGGC GGTAACTAAA 
ATGGGAATCA GTCTGAACGA TGTGCTGCCC TCACGCCCCT ATCTGCTGCC CCTGCTGATG 
ATCCATCCAC TTCGCGTCCA GAGTTTTTTC TACGAGATCC TGGCCGGAAA ATGGGTGCGC 
AATGGTCTCC AAATCAAGGG ACAAGCGATG ACCTATATCC AGGCGAACTT CTGCAACTCG 
ATGGCCGACA TGGACCTGTT CTTCCTGCAA ATCTGTGCCA CCAACCTGCC GCAGTACTTC 
TTTCTGCAGA ACACCATCGA GTTGTTCGAC GTAGGTCAAT GGCTGGAGAC AGCTCCCTTG 
AAGCAGCCTC AAAAGGCGGA GCAATCCTCG ATGCTGGAGG GATTCCTAAC CTTCTTGGCC 
ACCCTGGTGA CGAGTCGCAC TAATCTGGGA AACGATGAAG CCACCCAATG CATTATTGAG 
ATCAGTGCCT TGTTGGCTAC CGAGAACAAG ACGCATTCGC AGCTCCTTGA ACTAATGCCG 
GAGCGGTCGG GCAATGTGCA TACCAAAAAC TTTGAGACCT TCCTTAAGAA GCTATCGGTT 
TACAAGGCTC CGTCGAGTGG GAGCGAGAAC CTGGAGCAGG GTCTCTTCAC GCCCATCGAC 
GAGGTGTGGG AGAAGCACTA CGATCCGCTG CACGTCCTTT TGCGAGCAGT TCATCGTCGA 
GACTTTCAAT CATCGCTGGA CCGCTTTACA AACTATGTGA AGAGCAAGGA TAAGATGCCG 
GCAAGTGGTA ACCTATGGCC ACCCTTCCGA TTGCCTCATG CTCTGCCCGC AACGAGCTCC 
TTCAGTGATC CCTGCAAGAT TTTGAACTCG CGCATCTTTC ACTCCACCAT CCTGTCGATA 
TTTTTCCGTG CAGTTCACAC ACGTGATGTT TCCGAGCATC TGCTGGCTCT GGCTGTGTTC 
CTCTTAGAGA TTGCTGTGGA AACAAGTGAT GATGTTGGCA GTGGAACTGG AGATCGGGCC 
CCTCCTGCGC TGGCTGCTGT GGTGGAGTCA TCTAGCGGGC CGGGCTATCA TGGCTACGGC 
ACGGGACGGC ATGAGCCGCC CAAGCTTTTC CACTGCTATC CCACGGACAA TCTAAGCTGC 
AATCTGCGCC ACGTGGTCAA GAAAGTGTCG CTGAAGTCAC GCGATCCGCA AGTGATCACC 
TCCAGCTATC GGTCGAACCC ATTTTACTCA GATCTGGACT TTGAGGTTGA AGCGGATCCA 
GAGCAATCGA TGCGCATGAT TGGTCAAGGG GACCCCGAAG GAGATGAGGC AACGGGTGGA 
GCTGGACTGG GAATGGGAGC TCATAGCAGA AGGAATGTCT CTCAGGCGCT GGTGCCGATG 
AGAGTGCCCG GCATGGAGGT CGCCCTACCA CCGGATCTTT CAGTAGTTGC CGAAACCGGT 
GTGGTTATCC GCCAGGATAG CAACGAAGAT GATCTACTGA GAGAAGGCAA TCATGCCATG 
GATATGAGTC CACCGGCTGC TCTCGATTTC CACTTTCCGC TGCAGCAGAT CACTTTGCCG 
GAGAGCGGAA TGGAGGTGGC TATTCGGAGA GATCTTCTGC TAGCCGAAAC GAATAATATG 
GGCGCAATTG CAGGTGGAGC TGGAGGTGGC GCCAATGCAT CGGCCACACC ACCAGCAGGG 
GCTTCCAATG AGATGTTCTC GCCTACCACA CCCACAGGCA GCGGTATGCT CCTGCCTTTC 
CAGCGGGTTC AGCCTGTAGC CGTACCCAGT AGCGGTAATA TGGATATTGT ACCATCAAAT 
GCCATGGGTG CCGGCGGAAG TTTCACATCC GGCGTAAGTG GTAGCGGCAC TGGACGTAGA 
ATGAACTACG AAACGGGTGG AGCACGTAAG CGATCCGTGG ACATTGCCAT CGGTGGCAGC 
AATAAGGATG AGCTGCATTT GGACGAAAGC ATTTTGTCCC TGCTACTAAA GCTTCATTCT 
CAGCTCTCAG GCACACTAGA TAGCTTCTCG TTGAGCGACG GTGAAGATCA ATCATCTGAC 
GACGATTCGA CCATGGATGT GGACTGTAAC GAAGCCAGCA CCTCCATGGC AGCAGCGGAG 
AGCACTGCCC TGGCTGAGAG AGGACGCAGT AAGCGCAACT ACAAGAACAT TCATGTATCC 
TCCTCGCGCA TCGGTGACGG ACCATTCTTT ATTGGTAATC TGCTGCGAAA GATCGCCAAG 
CAGGATGAGC AGTGCGCCCA GAGCATCGAC GACATCCGTG CCAGACTCTG GCCGAATCAG 
CGTGAAAAAC AAGCAGAGGC GAAAGCACGC GAGGCCAAGG AGAAGGAGGA GCGCCGCAAA 
AAAGCACGTG AGCGCCAGCA GAAGATGATG CAAGACTTCG CCAACAAGCA GAAGCTCTTC 
ATGCAATCCG CTGCAGCGTC ATCCAGTGGC ATGGGCTACG GACCGGAGGA CGAGGACGAT 
GAGGAGTTGT ACGAGGAGCA GCCGCGTGAG AAAGAGTACG ATTGCATAAT CTGCAATTGC 
ACTACGCCCA GTACCGAATC GAATCCTATC GGCTTGGTCG TTCTGGTCGA GTCCAGCGGC 
ATTGTTGGTC ATCGGCGGCG CATCGCCGAG CGATTGCCGC TGCCGATAAA CGCGGAGGAT 
GAGTCCCGAC TGGCGCACAC CACTCGACTG GCGGCTGAAT TCAACAGGCG CACGGAACTG 
CTCAGTCTGA AGTTTGGCGA TGAGTCCTGG TATCTGTCCA ATAATATGGC CTACGACAAC 
GGTGTACATG TGCAGTCGTG TGGCCACCAC GTTCATCTCA GTTGCCTAGA GGCATACCTT 
AAGACGCTGT ACACCACCCA ACGTCAGCCA GTTCAGGATC GTGGCGAGTT CTACTGTCCC 
GTGTGCCGAC AGCTCTCAAA CTCGGTGCTG CCGCTGAGTC CACAACTGGA CAGACCCACG 
CACCTGGTCC GCTCTGGTAA TCAGCCATTC GAACGTCTGG TCGCCGATCT AACAGATCTG 
ATTAAAGAGA ACGAAACGAT ACCGCAGCCA ACAAAACTGA CAGAGGCCAT GGGCCATGCC 
ATGGAGGTGA TGACAAACAT TGCCCAGCGC AAGGTTAAAT GCTCCTCGAT AACGTTCCGC 
AAGCTGTTCA TTTTCGTTAC CTCGATTGCG CGTACAAACC TGGAGGCGGA GATCATCCAG 
CGGGGCGGAT CACTATGTAC AGCGAATGCC ACGCGGTACA AGCCAAAGCG GGAATGCATC 
GTACCGCTAC TGCACGTGCT ATCGGTACAT GTGCGTGTCT TGGTTGAATG GCCGCTGTGG 
AGCAGCTGGG CCTCGTTAGC TGGCCTGCCC GTTACCGCTA CGGAACCGTT GCCGGCGCAC 
TGCCTCGAAT TGATTCCCAG CATATTAGCG GATCCCATTG CTCTGCTGCT CAAGTTTATC 
CTGCTGGCGC CACTGCAACT CGATCAGGAT TACTTTACGT GCATGGTGAA AGTCATGTAC 
AATCTGCTGT ACTATCAAAT TGTGGTTCAG CTGTGCGTTA CTCTCACCGA TTTAGAGTGC 
GATCACATTG TCAAAGTTTA CGGCAGCACT AGCGTTGGCA GTGACAACTC TGCTGCGGAA 
TCCCAACAGC AGGAGTCTGC CGCCGGGACA ACGAACAACC GTCGCCGTGC TGGACAACAG 
CAGCAGAGCT CCTCGCAGCT AGGAAAGGCC ATGGCCCTGG TGCTGAGCCA GACAAATGAC 
CTAGTTCACT TGCGACGCGA CTGCATACCC AGTACCAGCA GCTCGGCGGC AGCGTCGGCG 
GCTGGTTCCT CCTCCACAAC GTCCACAAAC CATGGAGCTA GCGCAGCCAC AGCCTCCTCT 
GCCACGACGA TCGAGGTTAA TCTGAAGTCC ATGGAATTAC AGCTGCAGGC GCTATGCTTG 
CCGTTTTTGC GAGTAGCTGC CCTGCTGCGC CAGCATCTCT ATCGCCATGA GATGCCGGAG 
ATCTCGGCAC CGGGACTGGA ATTTGTGCGC CTTGTTTACT ATTTGGAACT CGTAACAGAT 
TCTATGGACT GGGATTGCTT TAATGCCAGC AAGGGGCTAT GCTTTATTCC CGGCACTGAG 
ACAACGCTGC CCCAGTTCTG GTGCCAGCAG TTGATGGAGG TGCGTCCGCC GGCCGATACG 
GTCAGAGAAT TGGTGCTCAT CAATCAGCAT TCGTTGTGGC AGCAGCCGCG ACTTCTTGAA 
TTGCCGCGCG AGTACGAACG CTTGTTCACG TACTACCATG AGCGGCCGTG CCTCAATTGC 
TATAAAGTGC CCAAGGAGAG CTCCATCTGC CTGCTGTGCG GGACGATCGT ATGCCTCAAG 
CAGAACTGCT GCGCGGAGAA TGACTGCTGC GAGGCTGTTC GCCATACGCT ATCCTGTGGC 
GGTGGCATAG GCATCTTTTT GGTGGTCACC TCCACGTACA TCATTGTTAT CCGCGGACGA 
CGCGCCTGCC TTTGGGGCTC ACTCTATCTG GACGACTTCG ATGAGGAGGA TCGCGATCTC 
AAGCGCGGCA AACCTTTGTA TTTGAGCAAA GACCGCTTCA ATCTCCTGGA GTCGCAGTGG 
CTGTCCCATA AGTTTGCTCA TACAAAACAC ACATGGGTGT TTCACCGGGA TCTCTTGTGA 
AATATGGAAG ATCTACTGCG CAGCATCCAG GAGCTGGTTG GCCAGATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259317.1
CDS	1074..7784
Translation	MDEDDNLSNADISIDDEEVVREQTHHPPMQEDQELDNEDGSSDVDLSSVYVVPPIVSAPSPASVRIAGGSTIGGGGVAAGAVPTTTDNSHSPFHDWDSSVAAAAAPPPPPPRTGPTTTTSSGTAAESGAASALSDDAGAKPSFFFGASSFSLRSRKEVAALINTECCRGSPTPDLDSIMDTLFNPGTPIDNLDNIEWIRWLIAGGRTPQEFVKIVRSYDNHAKCGLVWVPHVVAYRCRTCGISPCMSICRDCFKKGNHTNHDFNMFLSQAGGACDCGDTSVMKAEGFCSDHGINNRVNRDPVPNNLLAVAEAIMPKLLFRLLQHFREHSDTPLEVQAITSYSCEEFANMLIDLNNMGEIMRKVMTRTLINPEVYAFFMEAPCQDTRNGRFLKANREKYEDAVNRFPNPEPPDEYRDLPALGDKLVHTTLLEEFIFWTFKFEFPQTLVCFLLNMLPDQDYKEHLTRTFVMHYSRIPSVLEMSRDPDTLSNRVVHMSVQLFSNESLALKMVNELSLLHVMIISLKLMMSKILIQNTLHDPNKNFHFVIDCTRQVMKDHCYWPLVSDFNNVLSHESVALVFLRDDNLIDMWFQFLQMLQGMNVNVRETASHVEFEPNSYYAAFSCELEASAYPMWSIISHLQDGTHAHLAKKIINYCVTTLHEWLDSIYFMEARLSMEEMMQASFHFPLHRYLAAFVCQAVTKMGISLNDVLPSRPYLLPLLMIHPLRVQSFFYEILAGKWVRNGLQIKGQAMTYIQANFCNSMADMDLFFLQICATNLPQYFFLQNTIELFDVGQWLETAPLKQPQKAEQSSMLEGFLTFLATLVTSRTNLGNDEATQCIIEISALLATENKTHSQLLELMPERSGNVHTKNFETFLKKLSVYKAPSSGSENLEQGLFTPIDEVWEKHYDPLHVLLRAVHRRDFQSSLDRFTNYVKSKDKMPASGNLWPPFRLPHALPATSSFSDPCKILNSRIFHSTILSIFFRAVHTRDVSEHLLALAVFLLEIAVETSDDVGSGTGDRAPPALAAVVESSSGPGYHGYGTGRHEPPKLFHCYPTDNLSCNLRHVVKKVSLKSRDPQVITSSYRSNPFYSDLDFEVEADPEQSMRMIGQGDPEGDEATGGAGLGMGAHSRRNVSQALVPMRVPGMEVALPPDLSVVAETGVVIRQDSNEDDLLREGNHAMDMSPPAALDFHFPLQQITLPESGMEVAIRRDLLLAETNNMGAIAGGAGGGANASATPPAGASNEMFSPTTPTGSGMLLPFQRVQPVAVPSSGNMDIVPSNAMGAGGSFTSGVSGSGTGRRMNYETGGARKRSVDIAIGGSNKDELHLDESILSLLLKLHSQLSGTLDSFSLSDGEDQSSDDDSTMDVDCNEASTSMAAAESTALAERGRSKRNYKNIHVSSSRIGDGPFFIGNLLRKIAKQDEQCAQSIDDIRARLWPNQREKQAEAKAREAKEKEERRKKARERQQKMMQDFANKQKLFMQSAAASSSGMGYGPEDEDDEELYEEQPREKEYDCIICNCTTPSTESNPIGLVVLVESSGIVGHRRRIAERLPLPINAEDESRLAHTTRLAAEFNRRTELLSLKFGDESWYLSNNMAYDNGVHVQSCGHHVHLSCLEAYLKTLYTTQRQPVQDRGEFYCPVCRQLSNSVLPLSPQLDRPTHLVRSGNQPFERLVADLTDLIKENETIPQPTKLTEAMGHAMEVMTNIAQRKVKCSSITFRKLFIFVTSIARTNLEAEIIQRGGSLCTANATRYKPKRECIVPLLHVLSVHVRVLVEWPLWSSWASLAGLPVTATEPLPAHCLELIPSILADPIALLLKFILLAPLQLDQDYFTCMVKVMYNLLYYQIVVQLCVTLTDLECDHIVKVYGSTSVGSDNSAAESQQQESAAGTTNNRRRAGQQQQSSSQLGKAMALVLSQTNDLVHLRRDCIPSTSSSAAASAAGSSSTTSTNHGASAATASSATTIEVNLKSMELQLQALCLPFLRVAALLRQHLYRHEMPEISAPGLEFVRLVYYLELVTDSMDWDCFNASKGLCFIPGTETTLPQFWCQQLMEVRPPADTVRELVLINQHSLWQQPRLLELPREYERLFTYYHERPCLNCYKVPKESSICLLCGTIVCLKQNCCAENDCCEAVRHTLSCGGGIGIFLVVTSTYIIVIRGRRACLWGSLYLDDFDEEDRDLKRGKPLYLSKDRFNLLESQWLSHKFAHTKHTWVFHRDLL*NMEDLLRSIQELVGQI

Target ORF information:

RefSeq Version	NM_001272388.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ubr3 ubiquitin ligase (Ubr3), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272388.1

ORF Insert Sequence:

ATGGACGAGG ATGACAACCT GTCCAATGCC GACATAAGCA TAGATGACGA GGAGGTGGTG 
CGGGAGCAGA CCCACCATCC GCCCATGCAA GAGGATCAGG AGCTGGATAA CGAAGATGGG 
TCATCCGACG TGGATCTGTC TTCGGTGTAC GTCGTGCCAC CCATAGTCTC GGCACCATCG 
CCCGCATCAG TGCGCATCGC TGGTGGATCC ACAATTGGTG GCGGAGGGGT CGCAGCTGGA 
GCAGTTCCAA CAACCACCGA TAACTCGCAT TCCCCCTTCC ACGATTGGGA TTCCAGTGTG 
GCGGCCGCTG CTGCTCCACC GCCGCCACCG CCCAGGACAG GACCAACTAC AACTACAAGC 
TCCGGCACAG CCGCGGAATC GGGAGCCGCG TCCGCCTTAT CCGACGATGC CGGCGCCAAG 
CCAAGTTTCT TCTTTGGCGC CTCCTCGTTC AGTTTGCGCA GCCGCAAGGA GGTGGCCGCC 
CTGATCAACA CGGAATGCTG CCGTGGCAGC CCCACACCCG ATCTCGACTC CATAATGGAT 
ACCCTATTCA ATCCGGGGAC GCCCATCGAC AATCTGGACA ACATCGAGTG GATACGATGG 
CTCATTGCCG GCGGACGGAC GCCGCAGGAG TTTGTTAAAA TTGTGCGCAG CTATGACAAC 
CACGCCAAAT GCGGTCTGGT ATGGGTACCG CATGTGGTGG CCTACAGGTG CCGCACTTGC 
GGCATATCGC CCTGCATGTC CATATGCCGG GACTGCTTCA AGAAGGGCAA CCACACGAAC 
CACGACTTCA ATATGTTCCT GTCACAGGCG GGCGGTGCCT GCGATTGTGG TGACACCTCG 
GTCATGAAGG CGGAGGGTTT TTGCAGTGAT CATGGCATCA ATAATCGGGT GAATCGCGAT 
CCGGTGCCAA ACAATCTTTT GGCAGTGGCC GAGGCCATAA TGCCCAAGCT GCTCTTCCGC 
CTGCTGCAGC ACTTCCGTGA GCACAGCGAC ACCCCACTGG AGGTGCAAGC GATAACGTCG 
TACTCCTGCG AGGAGTTCGC CAACATGCTG ATCGATCTGA ACAACATGGG CGAAATAATG 
CGCAAAGTGA TGACGCGCAC GCTGATAAAT CCGGAGGTGT ATGCCTTCTT CATGGAAGCA 
CCGTGCCAGG ACACCCGGAA TGGACGCTTC TTGAAGGCGA ACCGAGAGAA GTACGAGGAT 
GCCGTCAATA GATTCCCAAA CCCAGAGCCA CCCGATGAGT ACAGAGATCT GCCCGCGCTG 
GGCGACAAAT TGGTCCACAC CACGCTACTC GAAGAGTTCA TCTTCTGGAC ATTTAAGTTC 
GAGTTTCCAC AGACTCTGGT CTGCTTTCTG CTGAACATGC TGCCTGATCA GGATTACAAG 
GAACACCTCA CCCGCACATT TGTGATGCAC TACAGCCGCA TACCGTCCGT GTTGGAAATG 
TCCCGTGATC CGGACACGCT CAGCAACCGG GTGGTCCACA TGAGCGTCCA ACTCTTCTCT 
AACGAAAGTC TGGCCCTCAA GATGGTCAAC GAGCTGTCGC TGCTGCACGT AATGATTATT 
AGTCTGAAGC TAATGATGTC CAAGATACTC ATACAGAATA CATTGCATGA TCCCAACAAG 
AATTTTCATT TCGTCATCGA CTGCACGCGT CAGGTGATGA AGGACCACTG CTACTGGCCA 
CTAGTCTCGG ACTTTAACAA CGTCCTTTCA CACGAATCGG TGGCCCTGGT CTTTCTGCGG 
GACGACAATC TCATCGATAT GTGGTTCCAG TTTCTCCAGA TGCTCCAGGG CATGAATGTC 
AATGTGCGCG AGACGGCTTC TCATGTGGAA TTCGAGCCAA ATAGCTACTA TGCGGCGTTC 
TCCTGTGAGC TTGAGGCCAG TGCCTATCCC ATGTGGTCGA TTATCTCGCA TCTGCAGGAT 
GGCACACATG CCCATCTGGC AAAGAAGATC ATCAACTACT GTGTGACGAC GCTGCACGAG 
TGGCTAGACT CTATTTACTT TATGGAGGCG CGTCTATCTA TGGAGGAGAT GATGCAGGCT 
TCGTTCCACT TTCCCCTGCA TCGTTATCTG GCTGCCTTTG TTTGCCAGGC GGTAACTAAA 
ATGGGAATCA GTCTGAACGA TGTGCTGCCC TCACGCCCCT ATCTGCTGCC CCTGCTGATG 
ATCCATCCAC TTCGCGTCCA GAGTTTTTTC TACGAGATCC TGGCCGGAAA ATGGGTGCGC 
AATGGTCTCC AAATCAAGGG ACAAGCGATG ACCTATATCC AGGCGAACTT CTGCAACTCG 
ATGGCCGACA TGGACCTGTT CTTCCTGCAA ATCTGTGCCA CCAACCTGCC GCAGTACTTC 
TTTCTGCAGA ACACCATCGA GTTGTTCGAC GTAGGTCAAT GGCTGGAGAC AGCTCCCTTG 
AAGCAGCCTC AAAAGGCGGA GCAATCCTCG ATGCTGGAGG GATTCCTAAC CTTCTTGGCC 
ACCCTGGTGA CGAGTCGCAC TAATCTGGGA AACGATGAAG CCACCCAATG CATTATTGAG 
ATCAGTGCCT TGTTGGCTAC CGAGAACAAG ACGCATTCGC AGCTCCTTGA ACTAATGCCG 
GAGCGGTCGG GCAATGTGCA TACCAAAAAC TTTGAGACCT TCCTTAAGAA GCTATCGGTT 
TACAAGGCTC CGTCGAGTGG GAGCGAGAAC CTGGAGCAGG GTCTCTTCAC GCCCATCGAC 
GAGGTGTGGG AGAAGCACTA CGATCCGCTG CACGTCCTTT TGCGAGCAGT TCATCGTCGA 
GACTTTCAAT CATCGCTGGA CCGCTTTACA AACTATGTGA AGAGCAAGGA TAAGATGCCG 
GCAAGTGGTA ACCTATGGCC ACCCTTCCGA TTGCCTCATG CTCTGCCCGC AACGAGCTCC 
TTCAGTGATC CCTGCAAGAT TTTGAACTCG CGCATCTTTC ACTCCACCAT CCTGTCGATA 
TTTTTCCGTG CAGTTCACAC ACGTGATGTT TCCGAGCATC TGCTGGCTCT GGCTGTGTTC 
CTCTTAGAGA TTGCTGTGGA AACAAGTGAT GATGTTGGCA GTGGAACTGG AGATCGGGCC 
CCTCCTGCGC TGGCTGCTGT GGTGGAGTCA TCTAGCGGGC CGGGCTATCA TGGCTACGGC 
ACGGGACGGC ATGAGCCGCC CAAGCTTTTC CACTGCTATC CCACGGACAA TCTAAGCTGC 
AATCTGCGCC ACGTGGTCAA GAAAGTGTCG CTGAAGTCAC GCGATCCGCA AGTGATCACC 
TCCAGCTATC GGTCGAACCC ATTTTACTCA GATCTGGACT TTGAGGTTGA AGCGGATCCA 
GAGCAATCGA TGCGCATGAT TGGTCAAGGG GACCCCGAAG GAGATGAGGC AACGGGTGGA 
GCTGGACTGG GAATGGGAGC TCATAGCAGA AGGAATGTCT CTCAGGCGCT GGTGCCGATG 
AGAGTGCCCG GCATGGAGGT CGCCCTACCA CCGGATCTTT CAGTAGTTGC CGAAACCGGT 
GTGGTTATCC GCCAGGATAG CAACGAAGAT GATCTACTGA GAGAAGGCAA TCATGCCATG 
GATATGAGTC CACCGGCTGC TCTCGATTTC CACTTTCCGC TGCAGCAGAT CACTTTGCCG 
GAGAGCGGAA TGGAGGTGGC TATTCGGAGA GATCTTCTGC TAGCCGAAAC GAATAATATG 
GGCGCAATTG CAGGTGGAGC TGGAGGTGGC GCCAATGCAT CGGCCACACC ACCAGCAGGG 
GCTTCCAATG AGATGTTCTC GCCTACCACA CCCACAGGCA GCGGTATGCT CCTGCCTTTC 
CAGCGGGTTC AGCCTGTAGC CGTACCCAGT AGCGGTAATA TGGATATTGT ACCATCAAAT 
GCCATGGGTG CCGGCGGAAG TTTCACATCC GGCGTAAGTG GTAGCGGCAC TGGACGTAGA 
ATGAACTACG AAACGGGTGG AGCACGTAAG CGATCCGTGG ACATTGCCAT CGGTGGCAGC 
AATAAGGATG AGCTGCATTT GGACGAAAGC ATTTTGTCCC TGCTACTAAA GCTTCATTCT 
CAGCTCTCAG GCACACTAGA TAGCTTCTCG TTGAGCGACG GTGAAGATCA ATCATCTGAC 
GACGATTCGA CCATGGATGT GGACTGTAAC GAAGCCAGCA CCTCCATGGC AGCAGCGGAG 
AGCACTGCCC TGGCTGAGAG AGGACGCAGT AAGCGCAACT ACAAGAACAT TCATGTATCC 
TCCTCGCGCA TCGGTGACGG ACCATTCTTT ATTGGTAATC TGCTGCGAAA GATCGCCAAG 
CAGGATGAGC AGTGCGCCCA GAGCATCGAC GACATCCGTG CCAGACTCTG GCCGAATCAG 
CGTGAAAAAC AAGCAGAGGC GAAAGCACGC GAGGCCAAGG AGAAGGAGGA GCGCCGCAAA 
AAAGCACGTG AGCGCCAGCA GAAGATGATG CAAGACTTCG CCAACAAGCA GAAGCTCTTC 
ATGCAATCCG CTGCAGCGTC ATCCAGTGGC ATGGGCTACG GACCGGAGGA CGAGGACGAT 
GAGGAGTTGT ACGAGGAGCA GCCGCGTGAG AAAGAGTACG ATTGCATAAT CTGCAATTGC 
ACTACGCCCA GTACCGAATC GAATCCTATC GGCTTGGTCG TTCTGGTCGA GTCCAGCGGC 
ATTGTTGGTC ATCGGCGGCG CATCGCCGAG CGATTGCCGC TGCCGATAAA CGCGGAGGAT 
GAGTCCCGAC TGGCGCACAC CACTCGACTG GCGGCTGAAT TCAACAGGCG CACGGAACTG 
CTCAGTCTGA AGTTTGGCGA TGAGTCCTGG TATCTGTCCA ATAATATGGC CTACGACAAC 
GGTGTACATG TGCAGTCGTG TGGCCACCAC GTTCATCTCA GTTGCCTAGA GGCATACCTT 
AAGACGCTGT ACACCACCCA ACGTCAGCCA GTTCAGGATC GTGGCGAGTT CTACTGTCCC 
GTGTGCCGAC AGCTCTCAAA CTCGGTGCTG CCGCTGAGTC CACAACTGGA CAGACCCACG 
CACCTGGTCC GCTCTGGTAA TCAGCCATTC GAACGTCTGG TCGCCGATCT AACAGATCTG 
ATTAAAGAGA ACGAAACGAT ACCGCAGCCA ACAAAACTGA CAGAGGCCAT GGGCCATGCC 
ATGGAGGTGA TGACAAACAT TGCCCAGCGC AAGGTTAAAT GCTCCTCGAT AACGTTCCGC 
AAGCTGTTCA TTTTCGTTAC CTCGATTGCG CGTACAAACC TGGAGGCGGA GATCATCCAG 
CGGGGCGGAT CACTATGTAC AGCGAATGCC ACGCGGTACA AGCCAAAGCG GGAATGCATC 
GTACCGCTAC TGCACGTGCT ATCGGTACAT GTGCGTGTCT TGGTTGAATG GCCGCTGTGG 
AGCAGCTGGG CCTCGTTAGC TGGCCTGCCC GTTACCGCTA CGGAACCGTT GCCGGCGCAC 
TGCCTCGAAT TGATTCCCAG CATATTAGCG GATCCCATTG CTCTGCTGCT CAAGTTTATC 
CTGCTGGCGC CACTGCAACT CGATCAGGAT TACTTTACGT GCATGGTGAA AGTCATGTAC 
AATCTGCTGT ACTATCAAAT TGTGGTTCAG CTGTGCGTTA CTCTCACCGA TTTAGAGTGC 
GATCACATTG TCAAAGTTTA CGGCAGCACT AGCGTTGGCA GTGACAACTC TGCTGCGGAA 
TCCCAACAGC AGGAGTCTGC CGCCGGGACA ACGAACAACC GTCGCCGTGC TGGACAACAG 
CAGCAGAGCT CCTCGCAGCT AGGAAAGGCC ATGGCCCTGG TGCTGAGCCA GACAAATGAC 
CTAGTTCACT TGCGACGCGA CTGCATACCC AGTACCAGCA GCTCGGCGGC AGCGTCGGCG 
GCTGGTTCCT CCTCCACAAC GTCCACAAAC CATGGAGCTA GCGCAGCCAC AGCCTCCTCT 
GCCACGACGA TCGAGGTTAA TCTGAAGTCC ATGGAATTAC AGCTGCAGGC GCTATGCTTG 
CCGTTTTTGC GAGTAGCTGC CCTGCTGCGC CAGCATCTCT ATCGCCATGA GATGCCGGAG 
ATCTCGGCAC CGGGACTGGA ATTTGTGCGC CTTGTTTACT ATTTGGAACT CGTAACAGAT 
TCTATGGACT GGGATTGCTT TAATGCCAGC AAGGGGCTAT GCTTTATTCC CGGCACTGAG 
ACAACGCTGC CCCAGTTCTG GTGCCAGCAG TTGATGGAGG TGCGTCCGCC GGCCGATACG 
GTCAGAGAAT TGGTGCTCAT CAATCAGCAT TCGTTGTGGC AGCAGCCGCG ACTTCTTGAA 
TTGCCGCGCG AGTACGAACG CTTGTTCACG TACTACCATG AGCGGCCGTG CCTCAATTGC 
TATAAAGTGC CCAAGGAGAG CTCCATCTGC CTGCTGTGCG GGACGATCGT ATGCCTCAAG 
CAGAACTGCT GCGCGGAGAA TGACTGCTGC GAGGCTGTTC GCCATACGCT ATCCTGTGGC 
GGTGGCATAG GCATCTTTTT GGTGGTCACC TCCACGTACA TCATTGTTAT CCGCGGACGA 
CGCGCCTGCC TTTGGGGCTC ACTCTATCTG GACGACTTCG ATGAGGAGGA TCGCGATCTC 
AAGCGCGGCA AACCTTTGTA TTTGAGCAAA GACCGCTTCA ATCTCCTGGA GTCGCAGTGG 
CTGTCCCATA AGTTTGCTCA TACAAAACAC ACATGGGTGT TTCACCGGGA TCTCTTGTGA 
AATATGGAAG ATCTACTGCG CAGCATCCAG GAGCTGGTTG GCCAGATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ubr3 ( NM_001272389.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259318.1 Drosophila melanogaster Ubr3 ubiquitin ligase (Ubr3), transcript variant C, mRNA.

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CloneID	OFa03021
Clone ID Related Accession (Same CDS sequence)	NM_001272389.1
Accession Version	NM_001272389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ubr3 ubiquitin ligase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGAGG ATGACAACCT GTCCAATGCC GACATAAGCA TAGATGACGA GGAGGTGGTG 
CGGGAGCAGA CCCACCATCC GCCCATGCAA GAGGATCAGG AGCTGGATAA CGAAGATGGG 
TCATCCGACG TGGATCTGTC TTCGGTGTAC GTCGTGCCAC CCATAGTCTC GGCACCATCG 
CCCGCATCAG TGCGCATCGC TGGTGGATCC ACAATTGGTG GCGGAGGGGT CGCAGCTGGA 
GCAGTTCCAA CAACCACCGA TAACTCGCAT TCCCCCTTCC ACGATTGGGA TTCCAGTGTG 
GCGGCCGCTG CTGCTCCACC GCCGCCACCG CCCAGGACAG GACCAACTAC AACTACAAGC 
TCCGGCACAG CCGCGGAATC GGGAGCCGCG TCCGCCTTAT CCGACGATGC CGGCGCCAAG 
CCAAGTTTCT TCTTTGGCGC CTCCTCGTTC AGTTTGCGCA GCCGCAAGGA GGTGGCCGCC 
CTGATCAACA CGGAATGCTG CCGTGGCAGC CCCACACCCG ATCTCGACTC CATAATGGAT 
ACCCTATTCA ATCCGGGGAC GCCCATCGAC AATCTGGACA ACATCGAGTG GATACGATGG 
CTCATTGCCG GCGGACGGAC GCCGCAGGAG TTTGTTAAAA TTGTGCGCAG CTATGACAAC 
CACGCCAAAT GCGGTCTGGT ATGGGTACCG CATGTGGTGG CCTACAGGTG CCGCACTTGC 
GGCATATCGC CCTGCATGTC CATATGCCGG GACTGCTTCA AGAAGGGCAA CCACACGAAC 
CACGACTTCA ATATGTTCCT GTCACAGGCG GGCGGTGCCT GCGATTGTGG TGACACCTCG 
GTCATGAAGG CGGAGGGTTT TTGCAGTGAT CATGGCATCA ATAATCGGGT GAATCGCGAT 
CCGGTGCCAA ACAATCTTTT GGCAGTGGCC GAGGCCATAA TGCCCAAGCT GCTCTTCCGC 
CTGCTGCAGC ACTTCCGTGA GCACAGCGAC ACCCCACTGG AGGTGCAAGC GATAACGTCG 
TACTCCTGCG AGGAGTTCGC CAACATGCTG ATCGATCTGA ACAACATGGG CGAAATAATG 
CGCAAAGTGA TGACGCGCAC GCTGATAAAT CCGGAGGTGT ATGCCTTCTT CATGGAAGCA 
CCGTGCCAGG ACACCCGGAA TGGACGCTTC TTGAAGGCGA ACCGAGAGAA GTACGAGGAT 
GCCGTCAATA GATTCCCAAA CCCAGAGCCA CCCGATGAGT ACAGAGATCT GCCCGCGCTG 
GGCGACAAAT TGGTCCACAC CACGCTACTC GAAGAGTTCA TCTTCTGGAC ATTTAAGTTC 
GAGTTTCCAC AGACTCTGGT CTGCTTTCTG CTGAACATGC TGCCTGATCA GGATTACAAG 
GAACACCTCA CCCGCACATT TGTGATGCAC TACAGCCGCA TACCGTCCGT GTTGGAAATG 
TCCCGTGATC CGGACACGCT CAGCAACCGG GTGGTCCACA TGAGCGTCCA ACTCTTCTCT 
AACGAAAGTC TGGCCCTCAA GATGGTCAAC GAGCTGTCGC TGCTGCACGT AATGATTATT 
AGTCTGAAGC TAATGATGTC CAAGATACTC ATACAGAATA CATTGCATGA TCCCAACAAG 
AATTTTCATT TCGTCATCGA CTGCACGCGT CAGGTGATGA AGGACCACTG CTACTGGCCA 
CTAGTCTCGG ACTTTAACAA CGTCCTTTCA CACGAATCGG TGGCCCTGGT CTTTCTGCGG 
GACGACAATC TCATCGATAT GTGGTTCCAG TTTCTCCAGA TGCTCCAGGG CATGAATGTC 
AATGTGCGCG AGACGGCTTC TCATGTGGAA TTCGAGCCAA ATAGCTACTA TGCGGCGTTC 
TCCTGTGAGC TTGAGGCCAG TGCCTATCCC ATGTGGTCGA TTATCTCGCA TCTGCAGGAT 
GGCACACATG CCCATCTGGC AAAGAAGATC ATCAACTACT GTGTGACGAC GCTGCACGAG 
TGGCTAGACT CTATTTACTT TATGGAGGCG CGTCTATCTA TGGAGGAGAT GATGCAGGCT 
TCGTTCCACT TTCCCCTGCA TCGTTATCTG GCTGCCTTTG TTTGCCAGGC GGTAACTAAA 
ATGGGAATCA GTCTGAACGA TGTGCTGCCC TCACGCCCCT ATCTGCTGCC CCTGCTGATG 
ATCCATCCAC TTCGCGTCCA GAGTTTTTTC TACGAGATCC TGGCCGGAAA ATGGGTGCGC 
AATGGTCTCC AAATCAAGGG ACAAGCGATG ACCTATATCC AGGCGAACTT CTGCAACTCG 
ATGGCCGACA TGGACCTGTT CTTCCTGCAA ATCTGTGCCA CCAACCTGCC GCAGTACTTC 
TTTCTGCAGA ACACCATCGA GTTGTTCGAC GTAGGTCAAT GGCTGGAGAC AGCTCCCTTG 
AAGCAGCCTC AAAAGGCGGA GCAATCCTCG ATGCTGGAGG GATTCCTAAC CTTCTTGGCC 
ACCCTGGTGA CGAGTCGCAC TAATCTGGGA AACGATGAAG CCACCCAATG CATTATTGAG 
ATCAGTGCCT TGTTGGCTAC CGAGAACAAG ACGCATTCGC AGCTCCTTGA ACTAATGCCG 
GAGCGGTCGG GCAATGTGCA TACCAAAAAC TTTGAGACCT TCCTTAAGAA GCTATCGGTT 
TACAAGGCTC CGTCGAGTGG GAGCGAGAAC CTGGAGCAGG GTCTCTTCAC GCCCATCGAC 
GAGGTGTGGG AGAAGCACTA CGATCCGCTG CACGTCCTTT TGCGAGCAGT TCATCGTCGA 
GACTTTCAAT CATCGCTGGA CCGCTTTACA AACTATGTGA AGAGCAAGGA TAAGATGCCG 
GCAAGTGGTA ACCTATGGCC ACCCTTCCGA TTGCCTCATG CTCTGCCCGC AACGAGCTCC 
TTCAGTGATC CCTGCAAGAT TTTGAACTCG CGCATCTTTC ACTCCACCAT CCTGTCGATA 
TTTTTCCGTG CAGTTCACAC ACGTGATGTT TCCGAGCATC TGCTGGCTCT GGCTGTGTTC 
CTCTTAGAGA TTGCTGTGGA AACAAGTGAT GATGTTGGCA GTGGAACTGG AGATCGGGCC 
CCTCCTGCGC TGGCTGCTGT GGTGGAGTCA TCTAGCGGGC CGGGCTATCA TGGCTACGGC 
ACGGGACGGC ATGAGCCGCC CAAGCTTTTC CACTGCTATC CCACGGACAA TCTAAGCTGC 
AATCTGCGCC ACGTGGTCAA GAAAGTGTCG CTGAAGTCAC GCGATCCGCA AGTGATCACC 
TCCAGCTATC GGTCGAACCC ATTTTACTCA GATCTGGACT TTGAGGTTGA AGCGGATCCA 
GAGCAATCGA TGCGCATGAT TGGTCAAGGG GACCCCGAAG GAGATGAGGC AACGGGTGGA 
GCTGGACTGG GAATGGGAGC TCATAGCAGA AGGAATGTCT CTCAGGCGCT GGTGCCGATG 
AGAGTGCCCG GCATGGAGGT CGCCCTACCA CCGGATCTTT CAGTAGTTGC CGAAACCGGT 
GTGGTTATCC GCCAGGATAG CAACGAAGAT GATCTACTGA GAGAAGGCAA TCATGCCATG 
GATATGAGTC CACCGGCTGC TCTCGATTTC CACTTTCCGC TGCAGCAGAT CACTTTGCCG 
GAGAGCGGAA TGGAGGTGGC TATTCGGAGA GATCTTCTGC TAGCCGAAAC GAATAATATG 
GGCGCAATTG CAGGTGGAGC TGGAGGTGGC GCCAATGCAT CGGCCACACC ACCAGCAGGG 
GCTTCCAATG AGATGTTCTC GCCTACCACA CCCACAGGCA GCGGTATGCT CCTGCCTTTC 
CAGCGGGTTC AGCCTGTAGC CGTACCCAGT AGCGGTAATA TGGATATTGT ACCATCAAAT 
GCCATGGGTG CCGGCGGAAG TTTCACATCC GGCGTAAGTG GTAGCGGCAC TGGACGTAGA 
ATGAACTACG AAACGGGTGG AGCACGTAAG CGATCCGTGG ACATTGCCAT CGGTGGCAGC 
AATAAGGATG AGCTGCATTT GGACGAAAGC ATTTTGTCCC TGCTACTAAA GCTTCATTCT 
CAGCTCTCAG GCACACTAGA TAGCTTCTCG TTGAGCGACG GTGAAGATCA ATCATCTGAC 
GACGATTCGA CCATGGATGT GGACTGTAAC GAAGCCAGCA CCTCCATGGC AGCAGCGGAG 
AGCACTGCCC TGGCTGAGAG AGGACGCAGT AAGCGCAACT ACAAGAACAT TCATGTATCC 
TCCTCGCGCA TCGGTGACGG ACCATTCTTT ATTGGTAATC TGCTGCGAAA GATCGCCAAG 
CAGGATGAGC AGTGCGCCCA GAGCATCGAC GACATCCGTG CCAGACTCTG GCCGAATCAG 
CGTGAAAAAC AAGCAGAGGC GAAAGCACGC GAGGCCAAGG AGAAGGAGGA GCGCCGCAAA 
AAAGCACGTG AGCGCCAGCA GAAGATGATG CAAGACTTCG CCAACAAGCA GAAGCTCTTC 
ATGCAATCCG CTGCAGCGTC ATCCAGTGGC ATGGGCTACG GACCGGAGGA CGAGGACGAT 
GAGGAGTTGT ACGAGGAGCA GCCGCGTGAG AAAGAGTACG ATTGCATAAT CTGCAATTGC 
ACTACGCCCA GTACCGAATC GAATCCTATC GGCTTGGTCG TTCTGGTCGA GTCCAGCGGC 
ATTGTTGGTC ATCGGCGGCG CATCGCCGAG CGATTGCCGC TGCCGATAAA CGCGGAGGAT 
GAGTCCCGAC TGGCGCACAC CACTCGACTG GCGGCTGAAT TCAACAGGCG CACGGAACTG 
CTCAGTCTGA AGTTTGGCGA TGAGTCCTGG TATCTGTCCA ATAATATGGC CTACGACAAC 
GGTGTACATG TGCAGTCGTG TGGCCACCAC GTTCATCTCA GTTGCCTAGA GGCATACCTT 
AAGACGCTGT ACACCACCCA ACGTCAGCCA GTTCAGGATC GTGGCGAGTT CTACTGTCCC 
GTGTGCCGAC AGCTCTCAAA CTCGGTGCTG CCGCTGAGTC CACAACTGGA CAGACCCACG 
CACCTGGTCC GCTCTGGTAA TCAGCCATTC GAACGTCTGG TCGCCGATCT AACAGATCTG 
ATTAAAGAGA ACGAAACGAT ACCGCCAACA AAACTGACAG AGGCCATGGG CCATGCCATG 
GAGGTGATGA CAAACATTGC CCAGCGCAAG GTTAAATGCT CCTCGATAAC GTTCCGCAAG 
CTGTTCATTT TCGTTACCTC GATTGCGCGT ACAAACCTGG AGGCGGAGAT CATCCAGCGG 
GGCGGATCAC TATGTACAGC GAATGCCACG CGGTACAAGC CAAAGCGGGA ATGCATCGTA 
CCGCTACTGC ACGTGCTATC GGTACATGTG CGTGTCTTGG TTGAATGGCC GCTGTGGAGC 
AGCTGGGCCT CGTTAGCTGG CCTGCCCGTT ACCGCTACGG AACCGTTGCC GGCGCACTGC 
CTCGAATTGA TTCCCAGCAT ATTAGCGGAT CCCATTGCTC TGCTGCTCAA GTTTATCCTG 
CTGGCGCCAC TGCAACTCGA TCAGGATTAC TTTACGTGCA TGGTGAAAGT CATGTACAAT 
CTGCTGTACT ATCAAATTGT GGTTCAGCTG TGCGTTACTC TCACCGATTT AGAGTGCGAT 
CACATTGTCA AAGTTTACGG CAGCACTAGC GTTGGCAGTG ACAACTCTGC TGCGGAATCC 
CAACAGCAGG AGTCTGCCGC CGGGACAACG AACAACCGTC GCCGTGCTGG ACAACAGCAG 
CAGAGCTCCT CGCAGCTAGG AAAGGCCATG GCCCTGGTGC TGAGCCAGAC AAATGACCTA 
GTTCACTTGC GACGCGACTG CATACCCAGT ACCAGCAGCT CGGCGGCAGC GTCGGCGGCT 
GGTTCCTCCT CCACAACGTC CACAAACCAT GGAGCTAGCG CAGCCACAGC CTCCTCTGCC 
ACGACGATCG AGGTTAATCT GAAGTCCATG GAATTACAGC TGCAGGCGCT ATGCTTGCCG 
TTTTTGCGAG TAGCTGCCCT GCTGCGCCAG CATCTCTATC GCCATGAGAT GCCGGAGATC 
TCGGCACCGG GACTGGAATT TGTGCGCCTT GTTTACTATT TGGAACTCGT AACAGATTCT 
ATGGACTGGG ATTGCTTTAA TGCCAGCAAG GGGCTATGCT TTATTCCCGG CACTGAGACA 
ACGCTGCCCC AGTTCTGGTG CCAGCAGTTG ATGGAGGTGC GTCCGCCGGC CGATACGGTC 
AGAGAATTGG TGCTCATCAA TCAGCATTCG TTGTGGCAGC AGCCGCGACT TCTTGAATTG 
CCGCGCGAGT ACGAACGCTT GTTCACGTAC TACCATGAGC GGCCGTGCCT CAATTGCTAT 
AAAGTGCCCA AGGAGAGCTC CATCTGCCTG CTGTGCGGGA CGATCGTATG CCTCAAGCAG 
AACTGCTGCG CGGAGAATGA CTGCTGCGAG GCTGTTCGCC ATACGCTATC CTGTGGCGGT 
GGCATAGGCA TCTTTTTGGT GGTCACCTCC ACGTACATCA TTGTTATCCG CGGACGACGC 
GCCTGCCTTT GGGGCTCACT CTATCTGGAC GACTTCGATG AGGAGGATCG CGATCTCAAG 
CGCGGCAAAC CTTTGTATTT GAGCAAAGAC CGCTTCAATC TCCTGGAGTC GCAGTGGCTG 
TCCCATAAGT TTGCTCATAC AAAACACACA TGGGTGTTTC ACCGGGATCT CTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259318.1
CDS	1014..7670
Translation	MDEDDNLSNADISIDDEEVVREQTHHPPMQEDQELDNEDGSSDVDLSSVYVVPPIVSAPSPASVRIAGGSTIGGGGVAAGAVPTTTDNSHSPFHDWDSSVAAAAAPPPPPPRTGPTTTTSSGTAAESGAASALSDDAGAKPSFFFGASSFSLRSRKEVAALINTECCRGSPTPDLDSIMDTLFNPGTPIDNLDNIEWIRWLIAGGRTPQEFVKIVRSYDNHAKCGLVWVPHVVAYRCRTCGISPCMSICRDCFKKGNHTNHDFNMFLSQAGGACDCGDTSVMKAEGFCSDHGINNRVNRDPVPNNLLAVAEAIMPKLLFRLLQHFREHSDTPLEVQAITSYSCEEFANMLIDLNNMGEIMRKVMTRTLINPEVYAFFMEAPCQDTRNGRFLKANREKYEDAVNRFPNPEPPDEYRDLPALGDKLVHTTLLEEFIFWTFKFEFPQTLVCFLLNMLPDQDYKEHLTRTFVMHYSRIPSVLEMSRDPDTLSNRVVHMSVQLFSNESLALKMVNELSLLHVMIISLKLMMSKILIQNTLHDPNKNFHFVIDCTRQVMKDHCYWPLVSDFNNVLSHESVALVFLRDDNLIDMWFQFLQMLQGMNVNVRETASHVEFEPNSYYAAFSCELEASAYPMWSIISHLQDGTHAHLAKKIINYCVTTLHEWLDSIYFMEARLSMEEMMQASFHFPLHRYLAAFVCQAVTKMGISLNDVLPSRPYLLPLLMIHPLRVQSFFYEILAGKWVRNGLQIKGQAMTYIQANFCNSMADMDLFFLQICATNLPQYFFLQNTIELFDVGQWLETAPLKQPQKAEQSSMLEGFLTFLATLVTSRTNLGNDEATQCIIEISALLATENKTHSQLLELMPERSGNVHTKNFETFLKKLSVYKAPSSGSENLEQGLFTPIDEVWEKHYDPLHVLLRAVHRRDFQSSLDRFTNYVKSKDKMPASGNLWPPFRLPHALPATSSFSDPCKILNSRIFHSTILSIFFRAVHTRDVSEHLLALAVFLLEIAVETSDDVGSGTGDRAPPALAAVVESSSGPGYHGYGTGRHEPPKLFHCYPTDNLSCNLRHVVKKVSLKSRDPQVITSSYRSNPFYSDLDFEVEADPEQSMRMIGQGDPEGDEATGGAGLGMGAHSRRNVSQALVPMRVPGMEVALPPDLSVVAETGVVIRQDSNEDDLLREGNHAMDMSPPAALDFHFPLQQITLPESGMEVAIRRDLLLAETNNMGAIAGGAGGGANASATPPAGASNEMFSPTTPTGSGMLLPFQRVQPVAVPSSGNMDIVPSNAMGAGGSFTSGVSGSGTGRRMNYETGGARKRSVDIAIGGSNKDELHLDESILSLLLKLHSQLSGTLDSFSLSDGEDQSSDDDSTMDVDCNEASTSMAAAESTALAERGRSKRNYKNIHVSSSRIGDGPFFIGNLLRKIAKQDEQCAQSIDDIRARLWPNQREKQAEAKAREAKEKEERRKKARERQQKMMQDFANKQKLFMQSAAASSSGMGYGPEDEDDEELYEEQPREKEYDCIICNCTTPSTESNPIGLVVLVESSGIVGHRRRIAERLPLPINAEDESRLAHTTRLAAEFNRRTELLSLKFGDESWYLSNNMAYDNGVHVQSCGHHVHLSCLEAYLKTLYTTQRQPVQDRGEFYCPVCRQLSNSVLPLSPQLDRPTHLVRSGNQPFERLVADLTDLIKENETIPPTKLTEAMGHAMEVMTNIAQRKVKCSSITFRKLFIFVTSIARTNLEAEIIQRGGSLCTANATRYKPKRECIVPLLHVLSVHVRVLVEWPLWSSWASLAGLPVTATEPLPAHCLELIPSILADPIALLLKFILLAPLQLDQDYFTCMVKVMYNLLYYQIVVQLCVTLTDLECDHIVKVYGSTSVGSDNSAAESQQQESAAGTTNNRRRAGQQQQSSSQLGKAMALVLSQTNDLVHLRRDCIPSTSSSAAASAAGSSSTTSTNHGASAATASSATTIEVNLKSMELQLQALCLPFLRVAALLRQHLYRHEMPEISAPGLEFVRLVYYLELVTDSMDWDCFNASKGLCFIPGTETTLPQFWCQQLMEVRPPADTVRELVLINQHSLWQQPRLLELPREYERLFTYYHERPCLNCYKVPKESSICLLCGTIVCLKQNCCAENDCCEAVRHTLSCGGGIGIFLVVTSTYIIVIRGRRACLWGSLYLDDFDEEDRDLKRGKPLYLSKDRFNLLESQWLSHKFAHTKHTWVFHRDLL

Target ORF information:

RefSeq Version	NM_001272389.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ubr3 ubiquitin ligase (Ubr3), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272389.1

ORF Insert Sequence:

ATGGACGAGG ATGACAACCT GTCCAATGCC GACATAAGCA TAGATGACGA GGAGGTGGTG 
CGGGAGCAGA CCCACCATCC GCCCATGCAA GAGGATCAGG AGCTGGATAA CGAAGATGGG 
TCATCCGACG TGGATCTGTC TTCGGTGTAC GTCGTGCCAC CCATAGTCTC GGCACCATCG 
CCCGCATCAG TGCGCATCGC TGGTGGATCC ACAATTGGTG GCGGAGGGGT CGCAGCTGGA 
GCAGTTCCAA CAACCACCGA TAACTCGCAT TCCCCCTTCC ACGATTGGGA TTCCAGTGTG 
GCGGCCGCTG CTGCTCCACC GCCGCCACCG CCCAGGACAG GACCAACTAC AACTACAAGC 
TCCGGCACAG CCGCGGAATC GGGAGCCGCG TCCGCCTTAT CCGACGATGC CGGCGCCAAG 
CCAAGTTTCT TCTTTGGCGC CTCCTCGTTC AGTTTGCGCA GCCGCAAGGA GGTGGCCGCC 
CTGATCAACA CGGAATGCTG CCGTGGCAGC CCCACACCCG ATCTCGACTC CATAATGGAT 
ACCCTATTCA ATCCGGGGAC GCCCATCGAC AATCTGGACA ACATCGAGTG GATACGATGG 
CTCATTGCCG GCGGACGGAC GCCGCAGGAG TTTGTTAAAA TTGTGCGCAG CTATGACAAC 
CACGCCAAAT GCGGTCTGGT ATGGGTACCG CATGTGGTGG CCTACAGGTG CCGCACTTGC 
GGCATATCGC CCTGCATGTC CATATGCCGG GACTGCTTCA AGAAGGGCAA CCACACGAAC 
CACGACTTCA ATATGTTCCT GTCACAGGCG GGCGGTGCCT GCGATTGTGG TGACACCTCG 
GTCATGAAGG CGGAGGGTTT TTGCAGTGAT CATGGCATCA ATAATCGGGT GAATCGCGAT 
CCGGTGCCAA ACAATCTTTT GGCAGTGGCC GAGGCCATAA TGCCCAAGCT GCTCTTCCGC 
CTGCTGCAGC ACTTCCGTGA GCACAGCGAC ACCCCACTGG AGGTGCAAGC GATAACGTCG 
TACTCCTGCG AGGAGTTCGC CAACATGCTG ATCGATCTGA ACAACATGGG CGAAATAATG 
CGCAAAGTGA TGACGCGCAC GCTGATAAAT CCGGAGGTGT ATGCCTTCTT CATGGAAGCA 
CCGTGCCAGG ACACCCGGAA TGGACGCTTC TTGAAGGCGA ACCGAGAGAA GTACGAGGAT 
GCCGTCAATA GATTCCCAAA CCCAGAGCCA CCCGATGAGT ACAGAGATCT GCCCGCGCTG 
GGCGACAAAT TGGTCCACAC CACGCTACTC GAAGAGTTCA TCTTCTGGAC ATTTAAGTTC 
GAGTTTCCAC AGACTCTGGT CTGCTTTCTG CTGAACATGC TGCCTGATCA GGATTACAAG 
GAACACCTCA CCCGCACATT TGTGATGCAC TACAGCCGCA TACCGTCCGT GTTGGAAATG 
TCCCGTGATC CGGACACGCT CAGCAACCGG GTGGTCCACA TGAGCGTCCA ACTCTTCTCT 
AACGAAAGTC TGGCCCTCAA GATGGTCAAC GAGCTGTCGC TGCTGCACGT AATGATTATT 
AGTCTGAAGC TAATGATGTC CAAGATACTC ATACAGAATA CATTGCATGA TCCCAACAAG 
AATTTTCATT TCGTCATCGA CTGCACGCGT CAGGTGATGA AGGACCACTG CTACTGGCCA 
CTAGTCTCGG ACTTTAACAA CGTCCTTTCA CACGAATCGG TGGCCCTGGT CTTTCTGCGG 
GACGACAATC TCATCGATAT GTGGTTCCAG TTTCTCCAGA TGCTCCAGGG CATGAATGTC 
AATGTGCGCG AGACGGCTTC TCATGTGGAA TTCGAGCCAA ATAGCTACTA TGCGGCGTTC 
TCCTGTGAGC TTGAGGCCAG TGCCTATCCC ATGTGGTCGA TTATCTCGCA TCTGCAGGAT 
GGCACACATG CCCATCTGGC AAAGAAGATC ATCAACTACT GTGTGACGAC GCTGCACGAG 
TGGCTAGACT CTATTTACTT TATGGAGGCG CGTCTATCTA TGGAGGAGAT GATGCAGGCT 
TCGTTCCACT TTCCCCTGCA TCGTTATCTG GCTGCCTTTG TTTGCCAGGC GGTAACTAAA 
ATGGGAATCA GTCTGAACGA TGTGCTGCCC TCACGCCCCT ATCTGCTGCC CCTGCTGATG 
ATCCATCCAC TTCGCGTCCA GAGTTTTTTC TACGAGATCC TGGCCGGAAA ATGGGTGCGC 
AATGGTCTCC AAATCAAGGG ACAAGCGATG ACCTATATCC AGGCGAACTT CTGCAACTCG 
ATGGCCGACA TGGACCTGTT CTTCCTGCAA ATCTGTGCCA CCAACCTGCC GCAGTACTTC 
TTTCTGCAGA ACACCATCGA GTTGTTCGAC GTAGGTCAAT GGCTGGAGAC AGCTCCCTTG 
AAGCAGCCTC AAAAGGCGGA GCAATCCTCG ATGCTGGAGG GATTCCTAAC CTTCTTGGCC 
ACCCTGGTGA CGAGTCGCAC TAATCTGGGA AACGATGAAG CCACCCAATG CATTATTGAG 
ATCAGTGCCT TGTTGGCTAC CGAGAACAAG ACGCATTCGC AGCTCCTTGA ACTAATGCCG 
GAGCGGTCGG GCAATGTGCA TACCAAAAAC TTTGAGACCT TCCTTAAGAA GCTATCGGTT 
TACAAGGCTC CGTCGAGTGG GAGCGAGAAC CTGGAGCAGG GTCTCTTCAC GCCCATCGAC 
GAGGTGTGGG AGAAGCACTA CGATCCGCTG CACGTCCTTT TGCGAGCAGT TCATCGTCGA 
GACTTTCAAT CATCGCTGGA CCGCTTTACA AACTATGTGA AGAGCAAGGA TAAGATGCCG 
GCAAGTGGTA ACCTATGGCC ACCCTTCCGA TTGCCTCATG CTCTGCCCGC AACGAGCTCC 
TTCAGTGATC CCTGCAAGAT TTTGAACTCG CGCATCTTTC ACTCCACCAT CCTGTCGATA 
TTTTTCCGTG CAGTTCACAC ACGTGATGTT TCCGAGCATC TGCTGGCTCT GGCTGTGTTC 
CTCTTAGAGA TTGCTGTGGA AACAAGTGAT GATGTTGGCA GTGGAACTGG AGATCGGGCC 
CCTCCTGCGC TGGCTGCTGT GGTGGAGTCA TCTAGCGGGC CGGGCTATCA TGGCTACGGC 
ACGGGACGGC ATGAGCCGCC CAAGCTTTTC CACTGCTATC CCACGGACAA TCTAAGCTGC 
AATCTGCGCC ACGTGGTCAA GAAAGTGTCG CTGAAGTCAC GCGATCCGCA AGTGATCACC 
TCCAGCTATC GGTCGAACCC ATTTTACTCA GATCTGGACT TTGAGGTTGA AGCGGATCCA 
GAGCAATCGA TGCGCATGAT TGGTCAAGGG GACCCCGAAG GAGATGAGGC AACGGGTGGA 
GCTGGACTGG GAATGGGAGC TCATAGCAGA AGGAATGTCT CTCAGGCGCT GGTGCCGATG 
AGAGTGCCCG GCATGGAGGT CGCCCTACCA CCGGATCTTT CAGTAGTTGC CGAAACCGGT 
GTGGTTATCC GCCAGGATAG CAACGAAGAT GATCTACTGA GAGAAGGCAA TCATGCCATG 
GATATGAGTC CACCGGCTGC TCTCGATTTC CACTTTCCGC TGCAGCAGAT CACTTTGCCG 
GAGAGCGGAA TGGAGGTGGC TATTCGGAGA GATCTTCTGC TAGCCGAAAC GAATAATATG 
GGCGCAATTG CAGGTGGAGC TGGAGGTGGC GCCAATGCAT CGGCCACACC ACCAGCAGGG 
GCTTCCAATG AGATGTTCTC GCCTACCACA CCCACAGGCA GCGGTATGCT CCTGCCTTTC 
CAGCGGGTTC AGCCTGTAGC CGTACCCAGT AGCGGTAATA TGGATATTGT ACCATCAAAT 
GCCATGGGTG CCGGCGGAAG TTTCACATCC GGCGTAAGTG GTAGCGGCAC TGGACGTAGA 
ATGAACTACG AAACGGGTGG AGCACGTAAG CGATCCGTGG ACATTGCCAT CGGTGGCAGC 
AATAAGGATG AGCTGCATTT GGACGAAAGC ATTTTGTCCC TGCTACTAAA GCTTCATTCT 
CAGCTCTCAG GCACACTAGA TAGCTTCTCG TTGAGCGACG GTGAAGATCA ATCATCTGAC 
GACGATTCGA CCATGGATGT GGACTGTAAC GAAGCCAGCA CCTCCATGGC AGCAGCGGAG 
AGCACTGCCC TGGCTGAGAG AGGACGCAGT AAGCGCAACT ACAAGAACAT TCATGTATCC 
TCCTCGCGCA TCGGTGACGG ACCATTCTTT ATTGGTAATC TGCTGCGAAA GATCGCCAAG 
CAGGATGAGC AGTGCGCCCA GAGCATCGAC GACATCCGTG CCAGACTCTG GCCGAATCAG 
CGTGAAAAAC AAGCAGAGGC GAAAGCACGC GAGGCCAAGG AGAAGGAGGA GCGCCGCAAA 
AAAGCACGTG AGCGCCAGCA GAAGATGATG CAAGACTTCG CCAACAAGCA GAAGCTCTTC 
ATGCAATCCG CTGCAGCGTC ATCCAGTGGC ATGGGCTACG GACCGGAGGA CGAGGACGAT 
GAGGAGTTGT ACGAGGAGCA GCCGCGTGAG AAAGAGTACG ATTGCATAAT CTGCAATTGC 
ACTACGCCCA GTACCGAATC GAATCCTATC GGCTTGGTCG TTCTGGTCGA GTCCAGCGGC 
ATTGTTGGTC ATCGGCGGCG CATCGCCGAG CGATTGCCGC TGCCGATAAA CGCGGAGGAT 
GAGTCCCGAC TGGCGCACAC CACTCGACTG GCGGCTGAAT TCAACAGGCG CACGGAACTG 
CTCAGTCTGA AGTTTGGCGA TGAGTCCTGG TATCTGTCCA ATAATATGGC CTACGACAAC 
GGTGTACATG TGCAGTCGTG TGGCCACCAC GTTCATCTCA GTTGCCTAGA GGCATACCTT 
AAGACGCTGT ACACCACCCA ACGTCAGCCA GTTCAGGATC GTGGCGAGTT CTACTGTCCC 
GTGTGCCGAC AGCTCTCAAA CTCGGTGCTG CCGCTGAGTC CACAACTGGA CAGACCCACG 
CACCTGGTCC GCTCTGGTAA TCAGCCATTC GAACGTCTGG TCGCCGATCT AACAGATCTG 
ATTAAAGAGA ACGAAACGAT ACCGCCAACA AAACTGACAG AGGCCATGGG CCATGCCATG 
GAGGTGATGA CAAACATTGC CCAGCGCAAG GTTAAATGCT CCTCGATAAC GTTCCGCAAG 
CTGTTCATTT TCGTTACCTC GATTGCGCGT ACAAACCTGG AGGCGGAGAT CATCCAGCGG 
GGCGGATCAC TATGTACAGC GAATGCCACG CGGTACAAGC CAAAGCGGGA ATGCATCGTA 
CCGCTACTGC ACGTGCTATC GGTACATGTG CGTGTCTTGG TTGAATGGCC GCTGTGGAGC 
AGCTGGGCCT CGTTAGCTGG CCTGCCCGTT ACCGCTACGG AACCGTTGCC GGCGCACTGC 
CTCGAATTGA TTCCCAGCAT ATTAGCGGAT CCCATTGCTC TGCTGCTCAA GTTTATCCTG 
CTGGCGCCAC TGCAACTCGA TCAGGATTAC TTTACGTGCA TGGTGAAAGT CATGTACAAT 
CTGCTGTACT ATCAAATTGT GGTTCAGCTG TGCGTTACTC TCACCGATTT AGAGTGCGAT 
CACATTGTCA AAGTTTACGG CAGCACTAGC GTTGGCAGTG ACAACTCTGC TGCGGAATCC 
CAACAGCAGG AGTCTGCCGC CGGGACAACG AACAACCGTC GCCGTGCTGG ACAACAGCAG 
CAGAGCTCCT CGCAGCTAGG AAAGGCCATG GCCCTGGTGC TGAGCCAGAC AAATGACCTA 
GTTCACTTGC GACGCGACTG CATACCCAGT ACCAGCAGCT CGGCGGCAGC GTCGGCGGCT 
GGTTCCTCCT CCACAACGTC CACAAACCAT GGAGCTAGCG CAGCCACAGC CTCCTCTGCC 
ACGACGATCG AGGTTAATCT GAAGTCCATG GAATTACAGC TGCAGGCGCT ATGCTTGCCG 
TTTTTGCGAG TAGCTGCCCT GCTGCGCCAG CATCTCTATC GCCATGAGAT GCCGGAGATC 
TCGGCACCGG GACTGGAATT TGTGCGCCTT GTTTACTATT TGGAACTCGT AACAGATTCT 
ATGGACTGGG ATTGCTTTAA TGCCAGCAAG GGGCTATGCT TTATTCCCGG CACTGAGACA 
ACGCTGCCCC AGTTCTGGTG CCAGCAGTTG ATGGAGGTGC GTCCGCCGGC CGATACGGTC 
AGAGAATTGG TGCTCATCAA TCAGCATTCG TTGTGGCAGC AGCCGCGACT TCTTGAATTG 
CCGCGCGAGT ACGAACGCTT GTTCACGTAC TACCATGAGC GGCCGTGCCT CAATTGCTAT 
AAAGTGCCCA AGGAGAGCTC CATCTGCCTG CTGTGCGGGA CGATCGTATG CCTCAAGCAG 
AACTGCTGCG CGGAGAATGA CTGCTGCGAG GCTGTTCGCC ATACGCTATC CTGTGGCGGT 
GGCATAGGCA TCTTTTTGGT GGTCACCTCC ACGTACATCA TTGTTATCCG CGGACGACGC 
GCCTGCCTTT GGGGCTCACT CTATCTGGAC GACTTCGATG AGGAGGATCG CGATCTCAAG 
CGCGGCAAAC CTTTGTATTT GAGCAAAGAC CGCTTCAATC TCCTGGAGTC GCAGTGGCTG 
TCCCATAAGT TTGCTCATAC AAAACACACA TGGGTGTTTC ACCGGGATCT CTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ubr3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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