UBR3 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010801932.2	XP_010800234.1	E3 ubiquitin-protein ligase UBR3 isoform X1
XM_015462259.1	XP_015317745.1	E3 ubiquitin-protein ligase UBR3 isoform X1
XM_015462260.1	XP_015317746.1	E3 ubiquitin-protein ligase UBR3 isoform X2
XM_015462261.1	XP_015317747.1	E3 ubiquitin-protein ligase UBR3 isoform X3
XM_015462262.1	XP_015317748.1	E3 ubiquitin-protein ligase UBR3 isoform X5
XM_010801933.3	XP_010800235.1	E3 ubiquitin-protein ligase UBR3 isoform X4
XM_010801935.3	XP_010800237.1	E3 ubiquitin-protein ligase UBR3 isoform X6
XM_010801935.2	XP_010800237.1	E3 ubiquitin-protein ligase UBR3 isoform X5
XM_010826431.2	XP_010824733.2	E3 ubiquitin-protein ligase UBR3 isoform X4
XM_005202390.3	XP_005202447.1	E3 ubiquitin-protein ligase UBR3 isoform X2
XM_010801934.2	XP_010800236.1	E3 ubiquitin-protein ligase UBR3 isoform X4
XM_010801934.3	XP_010800236.1	E3 ubiquitin-protein ligase UBR3 isoform X5
XM_010801933.2	XP_010800235.1	E3 ubiquitin-protein ligase UBR3 isoform X3
XM_010801932.3	XP_010800234.1	E3 ubiquitin-protein ligase UBR3 isoform X1
XM_024980234.1	XP_024836002.1	E3 ubiquitin-protein ligase UBR3 isoform X2
XM_005202390.4	XP_005202447.1	E3 ubiquitin-protein ligase UBR3 isoform X3
XM_024980246.1	XP_024836014.1	E3 ubiquitin-protein ligase UBR3 isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa227668	XM_024980234.1 Latest version!	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa227669	XM_024980246.1 Latest version!	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa16371	XM_015462260.1 Latest version!	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa16371	XM_005202390.4 Latest version!	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa53811	XM_015462259.1 Latest version!	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa53811	XM_010801932.3 Latest version!	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa53812	XM_010801933.3 Latest version!	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa53812	XM_015462261.1 Latest version!	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa53813	XM_010801934.3 Latest version!	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa53813	XM_010826431.2 Latest version!	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa53814	XM_015462262.1 Latest version!	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa53814	XM_010801935.3 Latest version!	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
View Old Accession Versions >>
OBa53814	XM_010801935.2	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa53813	XM_010801934.2	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa53812	XM_010801933.2	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa53811	XM_010801932.2	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa16371	XM_005202390.3	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

UBR3 ( XM_024980234.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836002.1 Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa227668
Clone ID Related Accession (Same CDS sequence)	XM_024980234.1
Accession Version	XM_024980234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase UBR3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCTGAT GTTCCATCAG AGAAAGACCT TAATAAAGTC 
CTTCAGCTTT TGGAACCTCA AATTTCCTTT TTAGAAGATC TAACTAAAAT GGGAGGAGCA 
ATGCGATCTG TTCTTACTCA GGTTTTAACA AACCAACAAA ACTACAAAGA TCTGACTTCT 
GGTCTTGGAG AAAATGCTTG TGCAAGGAAG AGTCATGAAA AATACCTTAT AGCTTTGAAG 
AGCTCTGGAC TTACATATCC TGAGGATAAA CTTGTATACG GTATACAAGA GCCATCTGCT 
GGCGCTAGCA CTCTGGCAGT TCAAGGTTTT GCAAGTGCAG CAGGAACATT GGGACAACTA 
GATTCTTCAG ATGAGGAGGA TCAGGATGGG AGTCAAGGTC TGGGCAAAAG AAAGAGGGTC 
AAACTAAGCA GTAACACCAA AGATCAATCC ATAATGGATG TTTTGAAACA TAAAAGTTTC 
TTAGAAGAGC TATTGTTTTG GACAATAAAG TATGAATTTC CGCAGAAGAT GGTGACTTTC 
TTACTCAACA TGCTTCCAGA TCAAGAGTAT AAGGTTGCTT TTACAAAAAC TTTTGTTCAG 
CATTATGCTT TCATTATGAA GACACTGAAG AAAAGTCATG AATCGGACAC TATGTCTAAC 
AGAATTGTGC ATATTAGTGT TCAGTTGTTC AGCAATGAAG AGCTAGCCAG ACAGGTAACA 
GAAGAATGTC AGCTGCTGGA TATTATGGTC ACTGTGCTGT TATACATGAT GGAAAGTTGC 
CTTATTAAAA GTGAGCTACA AGATGAAGAA AATAGTTTAC ACGTTGTAGT GAATTGTGGA 
GAAGCATTGC TGAAGAATAA CACTTATTGG CCTCTTGTCA GTGATTTTAT TAATATCCTT 
TCTCATCAAA GTGTGGCTAA GAGATTTTTG GAGGACCATG GTTTGTTAGT GACATGGATG 
AACTTTGTAT CTTTCTTTCA AGGTATGAAC TTAAATAAGC GAGAACTAAA TGAGCATGTG 
GAGTTTGAAT CTCAGACCTA CTATGCTGCC TTTGCTGCTG AACTTGAAGC CTGTGCACAG 
CCAATGTGGG GACTCTTATC ACACTGTAAA GTTAGGGAAA CTCAAGAATA TACCCGAAAT 
GTTGTTAGAT ATTGCCTTGA AGCTCTTCAA GACTGGTTTG ATGCTATTAA CTTCGTAGAT 
GAGCCAGCAC CTAACCAAGT TACTTTTCAT CTGCCACTAC ATCGTTATTA TGCTATGTTT 
TTGAGTAAGG CTGTGAAATG TCAAGAACTA GATTTGGATT CTGTTTTACC AGATCAGGAA 
ATGTTAATGA AACTAATGAT TCACCCACTC CAAATTCAGG CAAGTCTTGC TGAAATCCAT 
AGCAATATGT GGGTAAGAAA TGGTCTGCAG ATCAAAGGAC AAGCCATGAC CTATGTCCAG 
TCTCATTTCT GTAATTCCAT GATTGATCCT GACATTTACC TGTTACAGGT TTGTGCATCT 
AGACTTGATC CGGATTATTT TATTTCATCT GTCTTTGAAA GATTTAAGGT AGTGGATTTG 
TTGACAATGG CTTCACAACA TCAAAACACA GTACTTGATG CAGAGCATGA GAGATCCATG 
TTAGAAGGCG CTCTTACATT TCTTGTGATT CTTTTGAGTC TTCGTTTACA TTTAGGAATG 
TCTGATGATG AGATTCTGAG AGCAGAGATG GTAGCCCAGC TGTGTATGAA TGACAGAACA 
CACAGTTCAT TGCTGGACCT CATTCCAGAA AATCCAAATC CTAAAAGTGG TATTATTCCT 
GGAAGTTATA GCTTTGAATC AGTTTTATCA GCAGTAGCTG ATTTTAAGGC TCCTGTTTTT 
GAACCTGGAG GTTCTATGCA ACAAGGCATG TATACTCCTA AAGCTGAAGT CTGGGATCAT 
GAATTTGACC CCGTCATGGT CATTCTTCGA ACAGTTTACC GTAGAGACGT GCAGTCTGCA 
ATGGACAGAT ACACAGCATT TTTAAAACAG AGTGGAAAAT TCCCTGGAAA TCCTTGGCCA 
CCCTATAAGA AAAGGACACC ACTCCATCCC AGCTATAAAG GTCTCATGAG ACTTTTGCAC 
TGTAAAACTT TACACATTGT GCTATTCACT CTGCTTTACA AGATTTTGAT GGATCATCAA 
AATCTTTCAG AACATGTACT CTGTATGGTT TTATATCTGA TTGAATTAGG ACTTGAAAAT 
TCAGCTGAAG AGGAATCAGA TGAAGAGGCA TCAGCATGTG GACCTGAACG TTGTCATGAC 
AGTTGGTTTC CTGGTAGTAA CTTAGTATCG AATATGCGAC ACTTTATAAA TTATGTTAGA 
GTGAGAGTTC CAGAGACTGC TCCAGAAGTG AAGAGAGACT CACTTGCAAG TACCAGCTCT 
GATAGCTTGG GATCTTTACA AAGTTCACGT CGGCCTAAAG TTCTTCAAAG AGAGAATTCT 
GGTACAGCTC AGGTTTTCAG CTTAGTAGCA GAGCGCAGAA AGAAGTTTCA AGAGATCATC 
AATCGCAGTA ACAGTGAAGC AAATCAAGTG GTTCGTCCTA AAACTTCAAG TAAATGGTCT 
GCACCTGGTT CAGCTCCACA GTTAACTACA GCCATTTTGG AAATTAAAGA AAGTATATTG 
TCTTTGTTAA TTAAACTTCA CCACAAACTC TCAGGAAAAC AAAATTCCTA CTATCCTCCT 
TGGCTTGATG ACATAGAAAT TTTAATCCAA CCAGAAATTC CTAAATATAG TCATGGAGAT 
GGTATAACTG CAGTAGAAAG AATTTTACTA AAAGCTGCAT TGCAAAGCAG AATGAACAAA 
CGCATCATTG AAGAGATATG TAGAAAAGTG ACCCCTCCTG TACCACCCAA AAAGATTACT 
GCAGCAGAGA AGAAAACACT GGACAAAGAA GAAAGGCGAC AAAAAGCTAG AGAAAGACAG 
CAGAAATTGC TTGCAGAGTT TGCATCACGA CAGAAAAGCT TCATGGAAAC TGCCATGGAT 
GTTGATTCTC CAGAGAGTGA TATTCCTATG GAGATCACCA CAGCAGAACC TCAAGTTTCT 
GAGGCAGTAT ATGATTGTGT TATTTGTGGA CAGAGTGGGC CCTCCTCTGA AGACAGACCT 
ACGGGATTGG TTGTCCTGTT ACAAGCATCC TCAGTTTTGG GGCAGTGCCG TGACAACATT 
GAGCCAAAAA AGTTGCCTAT CTCTGAAGAG GAGCAGATAT ACCCCTGGGA TACATGTGCA 
GCAGTTCATG ACGTGAGGCT CTCATTATTA CAACGTTATT TTAAGGATAG TTCTTGTCTC 
TTGGCAGTAT CAATTGGCTG GGAAGGAGGT GTTTATGTAC AAACCTGTGG CCACACATTA 
CATATCGATT GCCATAAATC TTACATGGAA TCATTACGGA ATGACCAGGT TCTTCAGGGC 
TTCTCAGTGG ACAAAGGAGA ATTCACATGT CCTCTCTGTA GGCAGTTTGC TAACAGTGTT 
CTTCCTTGTT ATCCTGGAAG CAATGTTGAA AACCACCTTT GGCAGCGTCC TAGTAACAAA 
AGCATACAAG ATCTCATAAA GGAAGTGGAG GAGCTGCAAG GACGTCCGGG AGCTTTCCCA 
GTAAGCATCA GTTCAGAAAC CAACTTAAGT AAAGAAATGG AATCTGTAAT GAAAGATATA 
AAAAATACCA CTCAGAAGAA ATACAGAGAT TATAGCAAGA CTCCAGGCTC ACCAGATAAT 
GATTTTCTCT TTATGTATTC TGTTGCTAGA ACCAACTTGG AACTTGAATT AATTCATCGA 
GGAGGGAATT TATGTTCAGG TGGTGCAAGT ACAGCTGGTA AAAGGTCCTG TTTAAATCAG 
CTGTTCCATG TATTAGCCTT GCACATGCGA CTTTATAGCA TTGATTCTGA GTATAATCCT 
TGGAGAAAGC TCACACAGTT ACTAGAAGAG ATGAGTTCGC AACTGGGAAA TGAGGAACAA 
CAGCCTGAGG TTCCAATTCT TTATCATGAT GTTACATCCC TTTTGCTCAT CCAGATCTTA 
ATGATGCCTC AACCCTTACA CAAAGACCAC TTCACCTGCA TTGTGAAGGT GCTTTTTACC 
CTACTGTATA CACAAGGTCT TGTGGCACTT TCAGTTAAAT GCAATGAGGA AGATAGGTCA 
GCCTGGAAAC ACACAGGAGC CCTCAAAAAG AATACTTGTG ATGCAGAAAA GTCTTATGAA 
GTGTTATTGA GCTTTGTGAT AAGTGAACTG TCTAAAGGAA AGTTATACCA TGAAGAAGGA 
ACTCAGGAGT GTGCAACGAT TATCCCTGTT GCTTGGTCTC CTGAATCCAT GGAAAAATAC 
TTACAGGACT TCTGCTTACC ATTTCTCAGA ATCACCAGCC TTCTTCAGCA CCACCTTTTT 
GGGGAAGATT TACCTAGCTG CCAGGAAGAA GAAGAATTTT CAGTTCTTTC CAGCTGCCTG 
GGACTTCTGC CAACATTCTA CCAAACAGAA CATCCATTCA TCAGTGCCTC CTGTCTGGAT 
TGGCCAGTTC CGGCATTTGA TATTATATCT CAGTGGTGTT TTGAGATAAA TTCATTTACT 
GAAAGACATG CTGAACAAGG AAAGGCGTTG CTTATCCAAG AGTCAAAATG GAAATTGCCA 
CACCTTCTAC AGTTGCCTGA AAATTATAAC ACCATTTTCC AGTACTACCA CAGAAAAACC 
TGTAGTGTCT GCACCAAGGT TCCTAAAGAC CCTGCCGTTT GCCTTGTTTG TGGTACTTTT 
GTATGCCTGA AAGGACTTTG CTGCAAGCAA CAAAGTTACT GTGAATGTGT ATTGCATTCT 
CAGAACTGTG GTGCTGGAAC AGGGATTTTC CTTTTGATCA ATGCATCAGT GATTATCATC 
ATTCGAGGCC ACCGCTTCTG CCTCTGGGGT TCTGTGTATT TGGATGCTCA TGGAGAGGAA 
GACCGAGATC TTAGGCGAGG CAAGCCTCTC TACATTTGTA AAGAAAGATA CAAAGTTCTT 
GAACAACAGT GGATTTCTCA TACTTTTGAT CACATCAATA AAAGATGGGG TCCACATTAC 
AATGGGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836002.1
CDS	50..5761
Translation	MAAAAAAAVGGPQPPQAEVPAPGLALDKAATAAHLKAALSRPDNRAGAEELQALLERVLSAERPLAAAADGEEAAAAGGGGGPGAAEEEALEWCKCLLAGGGGYDEFCAAVRAYDPAALCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGDHTGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHQIKSSSNIPCVPKDLLMMSEFVLPRFIFCLIQYLREGYNEPADVPSEKDLNKVLQLLEPQISFLEDLTKMGGAMRSVLTQVLTNQQNYKDLTSGLGENACARKSHEKYLIALKSSGLTYPEDKLVYGIQEPSAGASTLAVQGFASAAGTLGQLDSSDEEDQDGSQGLGKRKRVKLSSNTKDQSIMDVLKHKSFLEELLFWTIKYEFPQKMVTFLLNMLPDQEYKVAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHGLLVTWMNFVSFFQGMNLNKRELNEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVRETQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAVKCQELDLDSVLPDQEMLMKLMIHPLQIQASLAEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNTVLDAEHERSMLEGALTFLVILLSLRLHLGMSDDEILRAEMVAQLCMNDRTHSSLLDLIPENPNPKSGIIPGSYSFESVLSAVADFKAPVFEPGGSMQQGMYTPKAEVWDHEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGKFPGNPWPPYKKRTPLHPSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNLSEHVLCMVLYLIELGLENSAEEESDEEASACGPERCHDSWFPGSNLVSNMRHFINYVRVRVPETAPEVKRDSLASTSSDSLGSLQSSRRPKVLQRENSGTAQVFSLVAERRKKFQEIINRSNSEANQVVRPKTSSKWSAPGSAPQLTTAILEIKESILSLLIKLHHKLSGKQNSYYPPWLDDIEILIQPEIPKYSHGDGITAVERILLKAALQSRMNKRIIEEICRKVTPPVPPKKITAAEKKTLDKEERRQKARERQQKLLAEFASRQKSFMETAMDVDSPESDIPMEITTAEPQVSEAVYDCVICGQSGPSSEDRPTGLVVLLQASSVLGQCRDNIEPKKLPISEEEQIYPWDTCAAVHDVRLSLLQRYFKDSSCLLAVSIGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCYPGSNVENHLWQRPSNKSIQDLIKEVEELQGRPGAFPVSISSETNLSKEMESVMKDIKNTTQKKYRDYSKTPGSPDNDFLFMYSVARTNLELELIHRGGNLCSGGASTAGKRSCLNQLFHVLALHMRLYSIDSEYNPWRKLTQLLEEMSSQLGNEEQQPEVPILYHDVTSLLLIQILMMPQPLHKDHFTCIVKVLFTLLYTQGLVALSVKCNEEDRSAWKHTGALKKNTCDAEKSYEVLLSFVISELSKGKLYHEEGTQECATIIPVAWSPESMEKYLQDFCLPFLRITSLLQHHLFGEDLPSCQEEEEFSVLSSCLGLLPTFYQTEHPFISASCLDWPVPAFDIISQWCFEINSFTERHAEQGKALLIQESKWKLPHLLQLPENYNTIFQYYHRKTCSVCTKVPKDPAVCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYKVLEQQWISHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_024980234.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980234.1

ORF Insert Sequence:

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCTGAT GTTCCATCAG AGAAAGACCT TAATAAAGTC 
CTTCAGCTTT TGGAACCTCA AATTTCCTTT TTAGAAGATC TAACTAAAAT GGGAGGAGCA 
ATGCGATCTG TTCTTACTCA GGTTTTAACA AACCAACAAA ACTACAAAGA TCTGACTTCT 
GGTCTTGGAG AAAATGCTTG TGCAAGGAAG AGTCATGAAA AATACCTTAT AGCTTTGAAG 
AGCTCTGGAC TTACATATCC TGAGGATAAA CTTGTATACG GTATACAAGA GCCATCTGCT 
GGCGCTAGCA CTCTGGCAGT TCAAGGTTTT GCAAGTGCAG CAGGAACATT GGGACAACTA 
GATTCTTCAG ATGAGGAGGA TCAGGATGGG AGTCAAGGTC TGGGCAAAAG AAAGAGGGTC 
AAACTAAGCA GTAACACCAA AGATCAATCC ATAATGGATG TTTTGAAACA TAAAAGTTTC 
TTAGAAGAGC TATTGTTTTG GACAATAAAG TATGAATTTC CGCAGAAGAT GGTGACTTTC 
TTACTCAACA TGCTTCCAGA TCAAGAGTAT AAGGTTGCTT TTACAAAAAC TTTTGTTCAG 
CATTATGCTT TCATTATGAA GACACTGAAG AAAAGTCATG AATCGGACAC TATGTCTAAC 
AGAATTGTGC ATATTAGTGT TCAGTTGTTC AGCAATGAAG AGCTAGCCAG ACAGGTAACA 
GAAGAATGTC AGCTGCTGGA TATTATGGTC ACTGTGCTGT TATACATGAT GGAAAGTTGC 
CTTATTAAAA GTGAGCTACA AGATGAAGAA AATAGTTTAC ACGTTGTAGT GAATTGTGGA 
GAAGCATTGC TGAAGAATAA CACTTATTGG CCTCTTGTCA GTGATTTTAT TAATATCCTT 
TCTCATCAAA GTGTGGCTAA GAGATTTTTG GAGGACCATG GTTTGTTAGT GACATGGATG 
AACTTTGTAT CTTTCTTTCA AGGTATGAAC TTAAATAAGC GAGAACTAAA TGAGCATGTG 
GAGTTTGAAT CTCAGACCTA CTATGCTGCC TTTGCTGCTG AACTTGAAGC CTGTGCACAG 
CCAATGTGGG GACTCTTATC ACACTGTAAA GTTAGGGAAA CTCAAGAATA TACCCGAAAT 
GTTGTTAGAT ATTGCCTTGA AGCTCTTCAA GACTGGTTTG ATGCTATTAA CTTCGTAGAT 
GAGCCAGCAC CTAACCAAGT TACTTTTCAT CTGCCACTAC ATCGTTATTA TGCTATGTTT 
TTGAGTAAGG CTGTGAAATG TCAAGAACTA GATTTGGATT CTGTTTTACC AGATCAGGAA 
ATGTTAATGA AACTAATGAT TCACCCACTC CAAATTCAGG CAAGTCTTGC TGAAATCCAT 
AGCAATATGT GGGTAAGAAA TGGTCTGCAG ATCAAAGGAC AAGCCATGAC CTATGTCCAG 
TCTCATTTCT GTAATTCCAT GATTGATCCT GACATTTACC TGTTACAGGT TTGTGCATCT 
AGACTTGATC CGGATTATTT TATTTCATCT GTCTTTGAAA GATTTAAGGT AGTGGATTTG 
TTGACAATGG CTTCACAACA TCAAAACACA GTACTTGATG CAGAGCATGA GAGATCCATG 
TTAGAAGGCG CTCTTACATT TCTTGTGATT CTTTTGAGTC TTCGTTTACA TTTAGGAATG 
TCTGATGATG AGATTCTGAG AGCAGAGATG GTAGCCCAGC TGTGTATGAA TGACAGAACA 
CACAGTTCAT TGCTGGACCT CATTCCAGAA AATCCAAATC CTAAAAGTGG TATTATTCCT 
GGAAGTTATA GCTTTGAATC AGTTTTATCA GCAGTAGCTG ATTTTAAGGC TCCTGTTTTT 
GAACCTGGAG GTTCTATGCA ACAAGGCATG TATACTCCTA AAGCTGAAGT CTGGGATCAT 
GAATTTGACC CCGTCATGGT CATTCTTCGA ACAGTTTACC GTAGAGACGT GCAGTCTGCA 
ATGGACAGAT ACACAGCATT TTTAAAACAG AGTGGAAAAT TCCCTGGAAA TCCTTGGCCA 
CCCTATAAGA AAAGGACACC ACTCCATCCC AGCTATAAAG GTCTCATGAG ACTTTTGCAC 
TGTAAAACTT TACACATTGT GCTATTCACT CTGCTTTACA AGATTTTGAT GGATCATCAA 
AATCTTTCAG AACATGTACT CTGTATGGTT TTATATCTGA TTGAATTAGG ACTTGAAAAT 
TCAGCTGAAG AGGAATCAGA TGAAGAGGCA TCAGCATGTG GACCTGAACG TTGTCATGAC 
AGTTGGTTTC CTGGTAGTAA CTTAGTATCG AATATGCGAC ACTTTATAAA TTATGTTAGA 
GTGAGAGTTC CAGAGACTGC TCCAGAAGTG AAGAGAGACT CACTTGCAAG TACCAGCTCT 
GATAGCTTGG GATCTTTACA AAGTTCACGT CGGCCTAAAG TTCTTCAAAG AGAGAATTCT 
GGTACAGCTC AGGTTTTCAG CTTAGTAGCA GAGCGCAGAA AGAAGTTTCA AGAGATCATC 
AATCGCAGTA ACAGTGAAGC AAATCAAGTG GTTCGTCCTA AAACTTCAAG TAAATGGTCT 
GCACCTGGTT CAGCTCCACA GTTAACTACA GCCATTTTGG AAATTAAAGA AAGTATATTG 
TCTTTGTTAA TTAAACTTCA CCACAAACTC TCAGGAAAAC AAAATTCCTA CTATCCTCCT 
TGGCTTGATG ACATAGAAAT TTTAATCCAA CCAGAAATTC CTAAATATAG TCATGGAGAT 
GGTATAACTG CAGTAGAAAG AATTTTACTA AAAGCTGCAT TGCAAAGCAG AATGAACAAA 
CGCATCATTG AAGAGATATG TAGAAAAGTG ACCCCTCCTG TACCACCCAA AAAGATTACT 
GCAGCAGAGA AGAAAACACT GGACAAAGAA GAAAGGCGAC AAAAAGCTAG AGAAAGACAG 
CAGAAATTGC TTGCAGAGTT TGCATCACGA CAGAAAAGCT TCATGGAAAC TGCCATGGAT 
GTTGATTCTC CAGAGAGTGA TATTCCTATG GAGATCACCA CAGCAGAACC TCAAGTTTCT 
GAGGCAGTAT ATGATTGTGT TATTTGTGGA CAGAGTGGGC CCTCCTCTGA AGACAGACCT 
ACGGGATTGG TTGTCCTGTT ACAAGCATCC TCAGTTTTGG GGCAGTGCCG TGACAACATT 
GAGCCAAAAA AGTTGCCTAT CTCTGAAGAG GAGCAGATAT ACCCCTGGGA TACATGTGCA 
GCAGTTCATG ACGTGAGGCT CTCATTATTA CAACGTTATT TTAAGGATAG TTCTTGTCTC 
TTGGCAGTAT CAATTGGCTG GGAAGGAGGT GTTTATGTAC AAACCTGTGG CCACACATTA 
CATATCGATT GCCATAAATC TTACATGGAA TCATTACGGA ATGACCAGGT TCTTCAGGGC 
TTCTCAGTGG ACAAAGGAGA ATTCACATGT CCTCTCTGTA GGCAGTTTGC TAACAGTGTT 
CTTCCTTGTT ATCCTGGAAG CAATGTTGAA AACCACCTTT GGCAGCGTCC TAGTAACAAA 
AGCATACAAG ATCTCATAAA GGAAGTGGAG GAGCTGCAAG GACGTCCGGG AGCTTTCCCA 
GTAAGCATCA GTTCAGAAAC CAACTTAAGT AAAGAAATGG AATCTGTAAT GAAAGATATA 
AAAAATACCA CTCAGAAGAA ATACAGAGAT TATAGCAAGA CTCCAGGCTC ACCAGATAAT 
GATTTTCTCT TTATGTATTC TGTTGCTAGA ACCAACTTGG AACTTGAATT AATTCATCGA 
GGAGGGAATT TATGTTCAGG TGGTGCAAGT ACAGCTGGTA AAAGGTCCTG TTTAAATCAG 
CTGTTCCATG TATTAGCCTT GCACATGCGA CTTTATAGCA TTGATTCTGA GTATAATCCT 
TGGAGAAAGC TCACACAGTT ACTAGAAGAG ATGAGTTCGC AACTGGGAAA TGAGGAACAA 
CAGCCTGAGG TTCCAATTCT TTATCATGAT GTTACATCCC TTTTGCTCAT CCAGATCTTA 
ATGATGCCTC AACCCTTACA CAAAGACCAC TTCACCTGCA TTGTGAAGGT GCTTTTTACC 
CTACTGTATA CACAAGGTCT TGTGGCACTT TCAGTTAAAT GCAATGAGGA AGATAGGTCA 
GCCTGGAAAC ACACAGGAGC CCTCAAAAAG AATACTTGTG ATGCAGAAAA GTCTTATGAA 
GTGTTATTGA GCTTTGTGAT AAGTGAACTG TCTAAAGGAA AGTTATACCA TGAAGAAGGA 
ACTCAGGAGT GTGCAACGAT TATCCCTGTT GCTTGGTCTC CTGAATCCAT GGAAAAATAC 
TTACAGGACT TCTGCTTACC ATTTCTCAGA ATCACCAGCC TTCTTCAGCA CCACCTTTTT 
GGGGAAGATT TACCTAGCTG CCAGGAAGAA GAAGAATTTT CAGTTCTTTC CAGCTGCCTG 
GGACTTCTGC CAACATTCTA CCAAACAGAA CATCCATTCA TCAGTGCCTC CTGTCTGGAT 
TGGCCAGTTC CGGCATTTGA TATTATATCT CAGTGGTGTT TTGAGATAAA TTCATTTACT 
GAAAGACATG CTGAACAAGG AAAGGCGTTG CTTATCCAAG AGTCAAAATG GAAATTGCCA 
CACCTTCTAC AGTTGCCTGA AAATTATAAC ACCATTTTCC AGTACTACCA CAGAAAAACC 
TGTAGTGTCT GCACCAAGGT TCCTAAAGAC CCTGCCGTTT GCCTTGTTTG TGGTACTTTT 
GTATGCCTGA AAGGACTTTG CTGCAAGCAA CAAAGTTACT GTGAATGTGT ATTGCATTCT 
CAGAACTGTG GTGCTGGAAC AGGGATTTTC CTTTTGATCA ATGCATCAGT GATTATCATC 
ATTCGAGGCC ACCGCTTCTG CCTCTGGGGT TCTGTGTATT TGGATGCTCA TGGAGAGGAA 
GACCGAGATC TTAGGCGAGG CAAGCCTCTC TACATTTGTA AAGAAAGATA CAAAGTTCTT 
GAACAACAGT GGATTTCTCA TACTTTTGAT CACATCAATA AAAGATGGGG TCCACATTAC 
AATGGGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR3 ( XM_024980246.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836014.1 Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X7, mRNA.

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CloneID	OBa227669
Clone ID Related Accession (Same CDS sequence)	XM_024980246.1
Accession Version	XM_024980246.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase UBR3 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCTGAT GTTCCATCAG AGAAAGACCT TAATAAAGTC 
CTTCAGCTTT TGGAACCTCA AATTTCCTTT TTAGAAGATC TAACTAAAAT GGGAGGAGCA 
ATGCGATCTG TTCTTACTCA GGTTTTAACA AACCAACAAA ACTACAAAGA TCTGACTTCT 
GGTCTTGGAG AAAATGCTTG TGCAAGGAAG AGTCATGAAA AATACCTTAT AGCTTTGAAG 
AGCTCTGGAC TTACATATCC TGAGGATAAA CTTGTATACG GTATACAAGA GCCATCTGCT 
GGCGCTAGCA CTCTGGCAGT TCAAGGTTTT GCAAGTGCAG CAGGAACATT GGGACAACTA 
GATTCTTCAG ATGAGGAGGA TCAGGATGGG AGTCAAGGTC TGGGCAAAAG AAAGAGGGTC 
AAACTAAGCA GTAACACCAA AGATCAATCC ATAATGGATG TTTTGAAACA TAAAAGTTTC 
TTAGAAGAGC TATTGTTTTG GACAATAAAG TATGAATTTC CGCAGAAGAT GGTGACTTTC 
TTACTCAACA TGCTTCCAGA TCAAGAGTAT AAGGTTGCTT TTACAAAAAC TTTTGTTCAG 
CATTATGCTT TCATTATGAA GACACTGAAG AAAAGTCATG AATCGGACAC TATGTCTAAC 
AGAATTGTGC ATATTAGTGT TCAGTTGTTC AGCAATGAAG AGCTAGCCAG ACAGGTAACA 
GAAGAATGTC AGCTGCTGGA TATTATGGTC ACTGTGCTGT TATACATGAT GGAAAGTTGC 
CTTATTAAAA GTGAGCTACA AGATGAAGAA AATAGTTTAC ACGTTGTAGT GAATTGTGGA 
GAAGCATTGC TGAAGAATAA CACTTATTGG CCTCTTGTCA GTGATTTTAT TAATATCCTT 
TCTCATCAAA GTGTGGCTAA GAGATTTTTG GAGGACCATG GTTTGTTAGT GACATGGATG 
AACTTTGTAT CTTTCTTTCA AGGTATGAAC TTAAATAAGC GAGAACTAAA TGAGCATGTG 
GAGTTTGAAT CTCAGACCTA CTATGCTGCC TTTGCTGCTG AACTTGAAGC CTGTGCACAG 
CCAATGTGGG GACTCTTATC ACACTGTAAA GTTAGGGAAA CTCAAGAATA TACCCGAAAT 
GTTGTTAGAT ATTGCCTTGA AGCTCTTCAA GACTGGTTTG ATGCTATTAA CTTCGTAGAT 
GAGCCAGCAC CTAACCAAGT TACTTTTCAT CTGCCACTAC ATCGTTATTA TGCTATGTTT 
TTGAGTAAGG CTGTGAAATG TCAAGAACTA GATTTGGATT CTGTTTTACC AGATCAGGAA 
ATGTTAATGA AACTAATGAT TCACCCACTC CAAATTCAGG CAAGTCTTGC TGAAATCCAT 
AGCAATATGT GGGTAAGAAA TGGTCTGCAG ATCAAAGGAC AAGCCATGAC CTATGTCCAG 
TCTCATTTCT GTAATTCCAT GATTGATCCT GACATTTACC TGTTACAGGT TTGTGCATCT 
AGACTTGATC CGGATTATTT TATTTCATCT GTCTTTGAAA GATTTAAGGT AGTGGATTTG 
TTGACAATGG CTTCACAACA TCAAAACACA GTACTTGATG CAGAGCATGA GAGATCCATG 
TTAGAAGGCG CTCTTACATT TCTTGTGATT CTTTTGAGTC TTCGTTTACA TTTAGGAATG 
TCTGATGATG AGATTCTGAG AGCAGAGATG GTAGCCCAGC TGTGTATGAA TGACAGAACA 
CACAGTTCAT TGCTGGACCT CATTCCAGAA AATCCAAATC CTAAAAGTGG TATTATTCCT 
GGAAGTTATA GCTTTGAATC AGTTTTATCA GCAGTAGCTG ATTTTAAGGC TCCTGTTTTT 
GAACCTGGAG GTTCTATGCA ACAAGGCATG TATACTCCTA AAGCTGAAGT CTGGGATCAT 
GAATTTGACC CCGTCATGGT CATTCTTCGA ACAGTTTACC GTAGAGACGT GCAGTCTGCA 
ATGGACAGAT ACACAGCATT TTTAAAACAG AGTGGAAAAT TCCCTGGAAA TCCTTGGCCA 
CCCTATAAGA AAAGGACACC ACTCCATCCC AGCTATAAAG GTCTCATGAG ACTTTTGCAC 
TGTAAAACTT TACACATTGT GCTATTCACT CTGCTTTACA AGATTTTGAT GGATCATCAA 
AATCTTTCAG AACATGTACT CTGTATGGTT TTATATCTGA TTGAATTAGG ACTTGAAAAT 
TCAGCTGAAG AGGAATCAGA TGAAGAGGCA TCAGCATGTG GACCTGAACG TTGTCATGAC 
AGTTGGTTTC CTGGTAGTAA CTTAGTATCG AATATGCGAC ACTTTATAAA TTATGTTAGA 
GTGAGAGTTC CAGAGACTGC TCCAGAAGTG AAGAGAGACT CACTTGCAAG TACCAGCTCT 
GATAGCTTGG GATCTTTACA AAATTCTGGT ACAGCTCAGG TTTTCAGCTT AGTAGCAGAG 
CGCAGAAAGA AGTTTCAAGA GATCATCAAT CGCAGTAACA GTGAAGCAAA TCAAGTGGTT 
CGTCCTAAAA CTTCAAGTAA ATGGTCTGCA CCTGGTTCAG CTCCACAGTT AACTACAGCC 
ATTTTGGAAA TTAAAGAAAG TATATTGTCT TTGTTAATTA AACTTCACCA CAAACTCTCA 
GGAAAACAAA ATTCCTACTA TCCTCCTTGG CTTGATGACA TAGAAATTTT AATCCAACCA 
GAAATTCCTA AATATAGTCA TGGAGATGGT ATAACTGCAG TAGAAAGAAT TTTACTAAAA 
GCTGCATTGC AAAGCAGAAT GAACAAACGC ATCATTGAAG AGATATGTAG AAAAGTGACC 
CCTCCTGTAC CACCCAAAAA GATTACTGCA GCAGAGAAGA AAACACTGGA CAAAGAAGAA 
AGGCGACAAA AAGCTAGAGA AAGACAGCAG AAATTGCTTG CAGAGTTTGC ATCACGACAG 
AAAAGCTTCA TGGAAACTGC CATGGATGTT GATTCTCCAG AGAGTGATAT TCCTATGGAG 
ATCACCACAG CAGAACCTCA AGTTTCTGAG GCAGTATATG ATTGTGTTAT TTGTGGACAG 
AGTGGGCCCT CCTCTGAAGA CAGACCTACG GGATTGGTTG TCCTGTTACA AGCATCCTCA 
GTTTTGGGGC AGTGCCGTGA CAACATTGAG CCAAAAAAGT TGCCTATCTC TGAAGAGGAG 
CAGATATACC CCTGGGATAC ATGTGCAGCA GTTCATGACG TGAGGCTCTC ATTATTACAA 
CGTTATTTTA AGGATAGTTC TTGTCTCTTG GCAGTATCAA TTGGCTGGGA AGGAGGTGTT 
TATGTACAAA CCTGTGGCCA CACATTACAT ATCGATTGCC ATAAATCTTA CATGGAATCA 
TTACGGAATG ACCAGGTTCT TCAGGGCTTC TCAGTGGACA AAGGAGAATT CACATGTCCT 
CTCTGTAGGC AGTTTGCTAA CAGTGTTCTT CCTTGTTATC CTGGAAGCAA TGTTGAAAAC 
CACCTTTGGC AGCGTCCTAG TAACAAAAGC ATACAAGATC TCATAAAGGA AGTGGAGGAG 
CTGCAAGGAC GTCCGGGAGC TTTCCCAGTA AGCATCAGTT CAGAAACCAA CTTAAGTAAA 
GAAATGGAAT CTGTAATGAA AGATATAAAA AATACCACTC AGAAGAAATA CAGAGATTAT 
AGCAAGACTC CAGGCTCACC AGATAATGAT TTTCTCTTTA TGTATTCTGT TGCTAGAACC 
AACTTGGAAC TTGAATTAAT TCATCGAGGA GGGAATTTAT GTTCAGGTGG TGCAAGTACA 
GCTGGTAAAA GGTCCTGTTT AAATCAGCTG TTCCATGTAT TAGCCTTGCA CATGCGACTT 
TATAGCATTG ATTCTGAGTA TAATCCTTGG AGAAAGCTCA CACAGTTACT AGAAGAGATG 
AGTTCGCAAC TGGGAAATGA GGAACAACAG CCTGAGGTTC CAATTCTTTA TCATGATGTT 
ACATCCCTTT TGCTCATCCA GATCTTAATG ATGCCTCAAC CCTTACACAA AGACCACTTC 
ACCTGCATTG TGAAGGTGCT TTTTACCCTA CTGTATACAC AAGGTCTTGT GGCACTTTCA 
GTTAAATGCA ATGAGGAAGA TAGGTCAGCC TGGAAACACA CAGGAGCCCT CAAAAAGAAT 
ACTTGTGATG CAGAAAAGTC TTATGAAGTG TTATTGAGCT TTGTGATAAG TGAACTGTCT 
AAAGGAAAGT TATACCATGA AGAAGGAACT CAGGAGTGTG CAACGATTAT CCCTGTTGCT 
TGGTCTCCTG AATCCATGGA AAAATACTTA CAGGACTTCT GCTTACCATT TCTCAGAATC 
ACCAGCCTTC TTCAGCACCA CCTTTTTGGG GAAGATTTAC CTAGCTGCCA GGAAGAAGAA 
GAATTTTCAG TTCTTTCCAG CTGCCTGGGA CTTCTGCCAA CATTCTACCA AACAGAACAT 
CCATTCATCA GTGCCTCCTG TCTGGATTGG CCAGTTCCGG CATTTGATAT TATATCTCAG 
TGGTGTTTTG AGATAAATTC ATTTACTGAA AGACATGCTG AACAAGGAAA GGCGTTGCTT 
ATCCAAGAGT CAAAATGGAA ATTGCCACAC CTTCTACAGT TGCCTGAAAA TTATAACACC 
ATTTTCCAGT ACTACCACAG AAAAACCTGT AGTGTCTGCA CCAAGGTTCC TAAAGACCCT 
GCCGTTTGCC TTGTTTGTGG TACTTTTGTA TGCCTGAAAG GACTTTGCTG CAAGCAACAA 
AGTTACTGTG AATGTGTATT GCATTCTCAG AACTGTGGTG CTGGAACAGG GATTTTCCTT 
TTGATCAATG CATCAGTGAT TATCATCATT CGAGGCCACC GCTTCTGCCT CTGGGGTTCT 
GTGTATTTGG ATGCTCATGG AGAGGAAGAC CGAGATCTTA GGCGAGGCAA GCCTCTCTAC 
ATTTGTAAAG AAAGATACAA AGTTCTTGAA CAACAGTGGA TTTCTCATAC TTTTGATCAC 
ATCAATAAAA GATGGGGTCC ACATTACAAT GGGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836014.1
CDS	49..5727
Translation	MAAAAAAAVGGPQPPQAEVPAPGLALDKAATAAHLKAALSRPDNRAGAEELQALLERVLSAERPLAAAADGEEAAAAGGGGGPGAAEEEALEWCKCLLAGGGGYDEFCAAVRAYDPAALCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGDHTGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHQIKSSSNIPCVPKDLLMMSEFVLPRFIFCLIQYLREGYNEPADVPSEKDLNKVLQLLEPQISFLEDLTKMGGAMRSVLTQVLTNQQNYKDLTSGLGENACARKSHEKYLIALKSSGLTYPEDKLVYGIQEPSAGASTLAVQGFASAAGTLGQLDSSDEEDQDGSQGLGKRKRVKLSSNTKDQSIMDVLKHKSFLEELLFWTIKYEFPQKMVTFLLNMLPDQEYKVAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHGLLVTWMNFVSFFQGMNLNKRELNEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVRETQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAVKCQELDLDSVLPDQEMLMKLMIHPLQIQASLAEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNTVLDAEHERSMLEGALTFLVILLSLRLHLGMSDDEILRAEMVAQLCMNDRTHSSLLDLIPENPNPKSGIIPGSYSFESVLSAVADFKAPVFEPGGSMQQGMYTPKAEVWDHEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGKFPGNPWPPYKKRTPLHPSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNLSEHVLCMVLYLIELGLENSAEEESDEEASACGPERCHDSWFPGSNLVSNMRHFINYVRVRVPETAPEVKRDSLASTSSDSLGSLQNSGTAQVFSLVAERRKKFQEIINRSNSEANQVVRPKTSSKWSAPGSAPQLTTAILEIKESILSLLIKLHHKLSGKQNSYYPPWLDDIEILIQPEIPKYSHGDGITAVERILLKAALQSRMNKRIIEEICRKVTPPVPPKKITAAEKKTLDKEERRQKARERQQKLLAEFASRQKSFMETAMDVDSPESDIPMEITTAEPQVSEAVYDCVICGQSGPSSEDRPTGLVVLLQASSVLGQCRDNIEPKKLPISEEEQIYPWDTCAAVHDVRLSLLQRYFKDSSCLLAVSIGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCYPGSNVENHLWQRPSNKSIQDLIKEVEELQGRPGAFPVSISSETNLSKEMESVMKDIKNTTQKKYRDYSKTPGSPDNDFLFMYSVARTNLELELIHRGGNLCSGGASTAGKRSCLNQLFHVLALHMRLYSIDSEYNPWRKLTQLLEEMSSQLGNEEQQPEVPILYHDVTSLLLIQILMMPQPLHKDHFTCIVKVLFTLLYTQGLVALSVKCNEEDRSAWKHTGALKKNTCDAEKSYEVLLSFVISELSKGKLYHEEGTQECATIIPVAWSPESMEKYLQDFCLPFLRITSLLQHHLFGEDLPSCQEEEEFSVLSSCLGLLPTFYQTEHPFISASCLDWPVPAFDIISQWCFEINSFTERHAEQGKALLIQESKWKLPHLLQLPENYNTIFQYYHRKTCSVCTKVPKDPAVCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYKVLEQQWISHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_024980246.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980246.1

ORF Insert Sequence:

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCTGAT GTTCCATCAG AGAAAGACCT TAATAAAGTC 
CTTCAGCTTT TGGAACCTCA AATTTCCTTT TTAGAAGATC TAACTAAAAT GGGAGGAGCA 
ATGCGATCTG TTCTTACTCA GGTTTTAACA AACCAACAAA ACTACAAAGA TCTGACTTCT 
GGTCTTGGAG AAAATGCTTG TGCAAGGAAG AGTCATGAAA AATACCTTAT AGCTTTGAAG 
AGCTCTGGAC TTACATATCC TGAGGATAAA CTTGTATACG GTATACAAGA GCCATCTGCT 
GGCGCTAGCA CTCTGGCAGT TCAAGGTTTT GCAAGTGCAG CAGGAACATT GGGACAACTA 
GATTCTTCAG ATGAGGAGGA TCAGGATGGG AGTCAAGGTC TGGGCAAAAG AAAGAGGGTC 
AAACTAAGCA GTAACACCAA AGATCAATCC ATAATGGATG TTTTGAAACA TAAAAGTTTC 
TTAGAAGAGC TATTGTTTTG GACAATAAAG TATGAATTTC CGCAGAAGAT GGTGACTTTC 
TTACTCAACA TGCTTCCAGA TCAAGAGTAT AAGGTTGCTT TTACAAAAAC TTTTGTTCAG 
CATTATGCTT TCATTATGAA GACACTGAAG AAAAGTCATG AATCGGACAC TATGTCTAAC 
AGAATTGTGC ATATTAGTGT TCAGTTGTTC AGCAATGAAG AGCTAGCCAG ACAGGTAACA 
GAAGAATGTC AGCTGCTGGA TATTATGGTC ACTGTGCTGT TATACATGAT GGAAAGTTGC 
CTTATTAAAA GTGAGCTACA AGATGAAGAA AATAGTTTAC ACGTTGTAGT GAATTGTGGA 
GAAGCATTGC TGAAGAATAA CACTTATTGG CCTCTTGTCA GTGATTTTAT TAATATCCTT 
TCTCATCAAA GTGTGGCTAA GAGATTTTTG GAGGACCATG GTTTGTTAGT GACATGGATG 
AACTTTGTAT CTTTCTTTCA AGGTATGAAC TTAAATAAGC GAGAACTAAA TGAGCATGTG 
GAGTTTGAAT CTCAGACCTA CTATGCTGCC TTTGCTGCTG AACTTGAAGC CTGTGCACAG 
CCAATGTGGG GACTCTTATC ACACTGTAAA GTTAGGGAAA CTCAAGAATA TACCCGAAAT 
GTTGTTAGAT ATTGCCTTGA AGCTCTTCAA GACTGGTTTG ATGCTATTAA CTTCGTAGAT 
GAGCCAGCAC CTAACCAAGT TACTTTTCAT CTGCCACTAC ATCGTTATTA TGCTATGTTT 
TTGAGTAAGG CTGTGAAATG TCAAGAACTA GATTTGGATT CTGTTTTACC AGATCAGGAA 
ATGTTAATGA AACTAATGAT TCACCCACTC CAAATTCAGG CAAGTCTTGC TGAAATCCAT 
AGCAATATGT GGGTAAGAAA TGGTCTGCAG ATCAAAGGAC AAGCCATGAC CTATGTCCAG 
TCTCATTTCT GTAATTCCAT GATTGATCCT GACATTTACC TGTTACAGGT TTGTGCATCT 
AGACTTGATC CGGATTATTT TATTTCATCT GTCTTTGAAA GATTTAAGGT AGTGGATTTG 
TTGACAATGG CTTCACAACA TCAAAACACA GTACTTGATG CAGAGCATGA GAGATCCATG 
TTAGAAGGCG CTCTTACATT TCTTGTGATT CTTTTGAGTC TTCGTTTACA TTTAGGAATG 
TCTGATGATG AGATTCTGAG AGCAGAGATG GTAGCCCAGC TGTGTATGAA TGACAGAACA 
CACAGTTCAT TGCTGGACCT CATTCCAGAA AATCCAAATC CTAAAAGTGG TATTATTCCT 
GGAAGTTATA GCTTTGAATC AGTTTTATCA GCAGTAGCTG ATTTTAAGGC TCCTGTTTTT 
GAACCTGGAG GTTCTATGCA ACAAGGCATG TATACTCCTA AAGCTGAAGT CTGGGATCAT 
GAATTTGACC CCGTCATGGT CATTCTTCGA ACAGTTTACC GTAGAGACGT GCAGTCTGCA 
ATGGACAGAT ACACAGCATT TTTAAAACAG AGTGGAAAAT TCCCTGGAAA TCCTTGGCCA 
CCCTATAAGA AAAGGACACC ACTCCATCCC AGCTATAAAG GTCTCATGAG ACTTTTGCAC 
TGTAAAACTT TACACATTGT GCTATTCACT CTGCTTTACA AGATTTTGAT GGATCATCAA 
AATCTTTCAG AACATGTACT CTGTATGGTT TTATATCTGA TTGAATTAGG ACTTGAAAAT 
TCAGCTGAAG AGGAATCAGA TGAAGAGGCA TCAGCATGTG GACCTGAACG TTGTCATGAC 
AGTTGGTTTC CTGGTAGTAA CTTAGTATCG AATATGCGAC ACTTTATAAA TTATGTTAGA 
GTGAGAGTTC CAGAGACTGC TCCAGAAGTG AAGAGAGACT CACTTGCAAG TACCAGCTCT 
GATAGCTTGG GATCTTTACA AAATTCTGGT ACAGCTCAGG TTTTCAGCTT AGTAGCAGAG 
CGCAGAAAGA AGTTTCAAGA GATCATCAAT CGCAGTAACA GTGAAGCAAA TCAAGTGGTT 
CGTCCTAAAA CTTCAAGTAA ATGGTCTGCA CCTGGTTCAG CTCCACAGTT AACTACAGCC 
ATTTTGGAAA TTAAAGAAAG TATATTGTCT TTGTTAATTA AACTTCACCA CAAACTCTCA 
GGAAAACAAA ATTCCTACTA TCCTCCTTGG CTTGATGACA TAGAAATTTT AATCCAACCA 
GAAATTCCTA AATATAGTCA TGGAGATGGT ATAACTGCAG TAGAAAGAAT TTTACTAAAA 
GCTGCATTGC AAAGCAGAAT GAACAAACGC ATCATTGAAG AGATATGTAG AAAAGTGACC 
CCTCCTGTAC CACCCAAAAA GATTACTGCA GCAGAGAAGA AAACACTGGA CAAAGAAGAA 
AGGCGACAAA AAGCTAGAGA AAGACAGCAG AAATTGCTTG CAGAGTTTGC ATCACGACAG 
AAAAGCTTCA TGGAAACTGC CATGGATGTT GATTCTCCAG AGAGTGATAT TCCTATGGAG 
ATCACCACAG CAGAACCTCA AGTTTCTGAG GCAGTATATG ATTGTGTTAT TTGTGGACAG 
AGTGGGCCCT CCTCTGAAGA CAGACCTACG GGATTGGTTG TCCTGTTACA AGCATCCTCA 
GTTTTGGGGC AGTGCCGTGA CAACATTGAG CCAAAAAAGT TGCCTATCTC TGAAGAGGAG 
CAGATATACC CCTGGGATAC ATGTGCAGCA GTTCATGACG TGAGGCTCTC ATTATTACAA 
CGTTATTTTA AGGATAGTTC TTGTCTCTTG GCAGTATCAA TTGGCTGGGA AGGAGGTGTT 
TATGTACAAA CCTGTGGCCA CACATTACAT ATCGATTGCC ATAAATCTTA CATGGAATCA 
TTACGGAATG ACCAGGTTCT TCAGGGCTTC TCAGTGGACA AAGGAGAATT CACATGTCCT 
CTCTGTAGGC AGTTTGCTAA CAGTGTTCTT CCTTGTTATC CTGGAAGCAA TGTTGAAAAC 
CACCTTTGGC AGCGTCCTAG TAACAAAAGC ATACAAGATC TCATAAAGGA AGTGGAGGAG 
CTGCAAGGAC GTCCGGGAGC TTTCCCAGTA AGCATCAGTT CAGAAACCAA CTTAAGTAAA 
GAAATGGAAT CTGTAATGAA AGATATAAAA AATACCACTC AGAAGAAATA CAGAGATTAT 
AGCAAGACTC CAGGCTCACC AGATAATGAT TTTCTCTTTA TGTATTCTGT TGCTAGAACC 
AACTTGGAAC TTGAATTAAT TCATCGAGGA GGGAATTTAT GTTCAGGTGG TGCAAGTACA 
GCTGGTAAAA GGTCCTGTTT AAATCAGCTG TTCCATGTAT TAGCCTTGCA CATGCGACTT 
TATAGCATTG ATTCTGAGTA TAATCCTTGG AGAAAGCTCA CACAGTTACT AGAAGAGATG 
AGTTCGCAAC TGGGAAATGA GGAACAACAG CCTGAGGTTC CAATTCTTTA TCATGATGTT 
ACATCCCTTT TGCTCATCCA GATCTTAATG ATGCCTCAAC CCTTACACAA AGACCACTTC 
ACCTGCATTG TGAAGGTGCT TTTTACCCTA CTGTATACAC AAGGTCTTGT GGCACTTTCA 
GTTAAATGCA ATGAGGAAGA TAGGTCAGCC TGGAAACACA CAGGAGCCCT CAAAAAGAAT 
ACTTGTGATG CAGAAAAGTC TTATGAAGTG TTATTGAGCT TTGTGATAAG TGAACTGTCT 
AAAGGAAAGT TATACCATGA AGAAGGAACT CAGGAGTGTG CAACGATTAT CCCTGTTGCT 
TGGTCTCCTG AATCCATGGA AAAATACTTA CAGGACTTCT GCTTACCATT TCTCAGAATC 
ACCAGCCTTC TTCAGCACCA CCTTTTTGGG GAAGATTTAC CTAGCTGCCA GGAAGAAGAA 
GAATTTTCAG TTCTTTCCAG CTGCCTGGGA CTTCTGCCAA CATTCTACCA AACAGAACAT 
CCATTCATCA GTGCCTCCTG TCTGGATTGG CCAGTTCCGG CATTTGATAT TATATCTCAG 
TGGTGTTTTG AGATAAATTC ATTTACTGAA AGACATGCTG AACAAGGAAA GGCGTTGCTT 
ATCCAAGAGT CAAAATGGAA ATTGCCACAC CTTCTACAGT TGCCTGAAAA TTATAACACC 
ATTTTCCAGT ACTACCACAG AAAAACCTGT AGTGTCTGCA CCAAGGTTCC TAAAGACCCT 
GCCGTTTGCC TTGTTTGTGG TACTTTTGTA TGCCTGAAAG GACTTTGCTG CAAGCAACAA 
AGTTACTGTG AATGTGTATT GCATTCTCAG AACTGTGGTG CTGGAACAGG GATTTTCCTT 
TTGATCAATG CATCAGTGAT TATCATCATT CGAGGCCACC GCTTCTGCCT CTGGGGTTCT 
GTGTATTTGG ATGCTCATGG AGAGGAAGAC CGAGATCTTA GGCGAGGCAA GCCTCTCTAC 
ATTTGTAAAG AAAGATACAA AGTTCTTGAA CAACAGTGGA TTTCTCATAC TTTTGATCAC 
ATCAATAAAA GATGGGGTCC ACATTACAAT GGGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR3 ( XM_015462260.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317746.1 Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X2, mRNA.

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CloneID	OBa16371
Clone ID Related Accession (Same CDS sequence)	XM_015462260.1 , XM_005202390.3 , XM_005202390.4
Accession Version	XM_015462260.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase UBR3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644584.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAGTTCA CGTCGGCCTA AAGTTCTTCA AAGAGAGAAT 
TCTGGTACAG CTCAGGTTTT CAGCTTAGTA GCAGAGCGCA GAAAGAAGTT TCAAGAGATC 
ATCAATCGCA GTAACAGTGA AGCAAATCAA GTGGTTCGTC CTAAAACTTC AAGTAAATGG 
TCTGCACCTG GTTCAGCTCC ACAGTTAACT ACAGCCATTT TGGAAATTAA AGAAAGTATA 
TTGTCTTTGT TAATTAAACT TCACCACAAA CTCTCAGGAA AACAAAATTC CTACTATCCT 
CCTTGGCTTG ATGACATAGA AATTTTAATC CAACCAGAAA TTCCTAAATA TAGTCATGGA 
GATGGTATAA CTGCAGTAGA AAGAATTTTA CTAAAAGCTG CATTGCAAAG CAGAATGAAC 
AAACGCATCA TTGAAGAGAT ATGTAGAAAA GTGACCCCTC CTGTACCACC CAAAAAGATT 
ACTGCAGCAG AGAAGAAAAC ACTGGACAAA GAAGAAAGGC GACAAAAAGC TAGAGAAAGA 
CAGCAGAAAT TGCTTGCAGA GTTTGCATCA CGACAGAAAA GCTTCATGGA AACTGCCATG 
GATGTTGATT CTCCAGAGAG TGATATTCCT ATGGAGATCA CCACAGCAGA ACCTCAAGTT 
TCTGAGGCAG TATATGATTG TGTTATTTGT GGACAGAGTG GGCCCTCCTC TGAAGACAGA 
CCTACGGGAT TGGTTGTCCT GTTACAAGCA TCCTCAGTTT TGGGGCAGTG CCGTGACAAC 
ATTGAGCCAA AAAAGTTGCC TATCTCTGAA GAGGAGCAGA TATACCCCTG GGATACATGT 
GCAGCAGTTC ATGACGTGAG GCTCTCATTA TTACAACGTT ATTTTAAGGA TAGTTCTTGT 
CTCTTGGCAG TATCAATTGG CTGGGAAGGA GGTGTTTATG TACAAACCTG TGGCCACACA 
TTACATATCG ATTGCCATAA ATCTTACATG GAATCATTAC GGAATGACCA GGTTCTTCAG 
GGCTTCTCAG TGGACAAAGG AGAATTCACA TGTCCTCTCT GTAGGCAGTT TGCTAACAGT 
GTTCTTCCTT GTTATCCTGG AAGCAATGTT GAAAACCACC TTTGGCAGCG TCCTAGTAAC 
AAAAGCATAC AAGATCTCAT AAAGGAAGTG GAGGAGCTGC AAGGACGTCC GGGAGCTTTC 
CCATCAGAAA CCAACTTAAG TAAAGAAATG GAATCTGTAA TGAAAGATAT AAAAAATACC 
ACTCAGAAGA AATACAGAGA TTATAGCAAG ACTCCAGGCT CACCAGATAA TGATTTTCTC 
TTTATGTATT CTGTTGCTAG AACCAACTTG GAACTTGAAT TAATTCATCG AGGAGGGAAT 
TTATGTTCAG GTGGTGCAAG TACAGCTGGT AAAAGGTCCT GTTTAAATCA GCTGTTCCAT 
GTATTAGCCT TGCACATGCG ACTTTATAGC ATTGATTCTG AGTATAATCC TTGGAGAAAG 
CTCACACAGT TACTAGAAGA GATGAGTTCG CAACTGGGAA ATGAGGAACA ACAGCCTGAG 
GTTCCAATTC TTTATCATGA TGTTACATCC CTTTTGCTCA TCCAGATCTT AATGATGCCT 
CAACCCTTAC ACAAAGACCA CTTCACCTGC ATTGTGAAGG TGCTTTTTAC CCTACTGTAT 
ACACAAGGTC TTGTGGCACT TTCAGTTAAA TGCAATGAGG AAGATAGGTC AGCCTGGAAA 
CACACAGGAG CCCTCAAAAA GAATACTTGT GATGCAGAAA AGTCTTATGA AGTGTTATTG 
AGCTTTGTGA TAAGTGAACT GTCTAAAGGA AAGTTATACC ATGAAGAAGG AACTCAGGAG 
TGTGCAACGA TTATCCCTGT TGCTTGGTCT CCTGAATCCA TGGAAAAATA CTTACAGGAC 
TTCTGCTTAC CATTTCTCAG AATCACCAGC CTTCTTCAGC ACCACCTTTT TGGGGAAGAT 
TTACCTAGCT GCCAGGAAGA AGAAGAATTT TCAGTTCTTT CCAGCTGCCT GGGACTTCTG 
CCAACATTCT ACCAAACAGA ACATCCATTC ATCAGTGCCT CCTGTCTGGA TTGGCCAGTT 
CCGGCATTTG ATATTATATC TCAGTGGTGT TTTGAGATAA ATTCATTTAC TGAAAGACAT 
GCTGAACAAG GAAAGGCGTT GCTTATCCAA GAGTCAAAAT GGAAATTGCC ACACCTTCTA 
CAGTTGCCTG AAAATTATAA CACCATTTTC CAGTACTACC ACAGAAAAAC CTGTAGTGTC 
TGCACCAAGG TTCCTAAAGA CCCTGCCGTT TGCCTTGTTT GTGGTACTTT TGTATGCCTG 
AAAGGACTTT GCTGCAAGCA ACAAAGTTAC TGTGAATGTG TATTGCATTC TCAGAACTGT 
GGTGCTGGAA CAGGGATTTT CCTTTTGATC AATGCATCAG TGATTATCAT CATTCGAGGC 
CACCGCTTCT GCCTCTGGGG TTCTGTGTAT TTGGATGCTC ATGGAGAGGA AGACCGAGAT 
CTTAGGCGAG GCAAGCCTCT CTACATTTGT AAAGAAAGAT ACAAAGTTCT TGAACAACAG 
TGGATTTCTC ATACTTTTGA TCACATCAAT AAAAGATGGG GTCCACATTA CAATGGGCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317746.1
CDS	40..5742
Translation	MAAAAAAAVGGPQPPQAEVPAPGLALDKAATAAHLKAALSRPDNRAGAEELQALLERVLSAERPLAAAADGEEAAAAGGGGGPGAAEEEALEWCKCLLAGGGGYDEFCAAVRAYDPAALCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGDHTGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHQIKSSSNIPCVPKDLLMMSEFVLPRFIFCLIQYLREGYNEPAADVPSEKDLNKVLQLLEPQISFLEDLTKMGGAMRSVLTQVLTNQQNYKDLTSGLGENACARKSHEKYLIALKSSGLTYPEDKLVYGIQEPSAGASTLAVQGFASAAGTLGQLDSSDEEDQDGSQGLGKRKRVKLSSNTKDQSIMDVLKHKSFLEELLFWTIKYEFPQKMVTFLLNMLPDQEYKVAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHGLLVTWMNFVSFFQGMNLNKRELNEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVRETQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAVKCQELDLDSVLPDQEMLMKLMIHPLQIQASLAEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNTVLDAEHERSMLEGALTFLVILLSLRLHLGMSDDEILRAEMVAQLCMNDRTHSSLLDLIPENPNPKSGIIPGSYSFESVLSAVADFKAPVFEPGGSMQQGMYTPKAEVWDHEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGKFPGNPWPPYKKRTPLHPSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNLSEHVLCMVLYLIELGLENSAEEESDEEASACGPERCHDSWFPGSNLVSNMRHFINYVRVRVPETAPEVKRDSLASTSSDSLGSLQSSRRPKVLQRENSGTAQVFSLVAERRKKFQEIINRSNSEANQVVRPKTSSKWSAPGSAPQLTTAILEIKESILSLLIKLHHKLSGKQNSYYPPWLDDIEILIQPEIPKYSHGDGITAVERILLKAALQSRMNKRIIEEICRKVTPPVPPKKITAAEKKTLDKEERRQKARERQQKLLAEFASRQKSFMETAMDVDSPESDIPMEITTAEPQVSEAVYDCVICGQSGPSSEDRPTGLVVLLQASSVLGQCRDNIEPKKLPISEEEQIYPWDTCAAVHDVRLSLLQRYFKDSSCLLAVSIGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCYPGSNVENHLWQRPSNKSIQDLIKEVEELQGRPGAFPSETNLSKEMESVMKDIKNTTQKKYRDYSKTPGSPDNDFLFMYSVARTNLELELIHRGGNLCSGGASTAGKRSCLNQLFHVLALHMRLYSIDSEYNPWRKLTQLLEEMSSQLGNEEQQPEVPILYHDVTSLLLIQILMMPQPLHKDHFTCIVKVLFTLLYTQGLVALSVKCNEEDRSAWKHTGALKKNTCDAEKSYEVLLSFVISELSKGKLYHEEGTQECATIIPVAWSPESMEKYLQDFCLPFLRITSLLQHHLFGEDLPSCQEEEEFSVLSSCLGLLPTFYQTEHPFISASCLDWPVPAFDIISQWCFEINSFTERHAEQGKALLIQESKWKLPHLLQLPENYNTIFQYYHRKTCSVCTKVPKDPAVCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYKVLEQQWISHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_015462260.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462260.1

ORF Insert Sequence:

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAGTTCA CGTCGGCCTA AAGTTCTTCA AAGAGAGAAT 
TCTGGTACAG CTCAGGTTTT CAGCTTAGTA GCAGAGCGCA GAAAGAAGTT TCAAGAGATC 
ATCAATCGCA GTAACAGTGA AGCAAATCAA GTGGTTCGTC CTAAAACTTC AAGTAAATGG 
TCTGCACCTG GTTCAGCTCC ACAGTTAACT ACAGCCATTT TGGAAATTAA AGAAAGTATA 
TTGTCTTTGT TAATTAAACT TCACCACAAA CTCTCAGGAA AACAAAATTC CTACTATCCT 
CCTTGGCTTG ATGACATAGA AATTTTAATC CAACCAGAAA TTCCTAAATA TAGTCATGGA 
GATGGTATAA CTGCAGTAGA AAGAATTTTA CTAAAAGCTG CATTGCAAAG CAGAATGAAC 
AAACGCATCA TTGAAGAGAT ATGTAGAAAA GTGACCCCTC CTGTACCACC CAAAAAGATT 
ACTGCAGCAG AGAAGAAAAC ACTGGACAAA GAAGAAAGGC GACAAAAAGC TAGAGAAAGA 
CAGCAGAAAT TGCTTGCAGA GTTTGCATCA CGACAGAAAA GCTTCATGGA AACTGCCATG 
GATGTTGATT CTCCAGAGAG TGATATTCCT ATGGAGATCA CCACAGCAGA ACCTCAAGTT 
TCTGAGGCAG TATATGATTG TGTTATTTGT GGACAGAGTG GGCCCTCCTC TGAAGACAGA 
CCTACGGGAT TGGTTGTCCT GTTACAAGCA TCCTCAGTTT TGGGGCAGTG CCGTGACAAC 
ATTGAGCCAA AAAAGTTGCC TATCTCTGAA GAGGAGCAGA TATACCCCTG GGATACATGT 
GCAGCAGTTC ATGACGTGAG GCTCTCATTA TTACAACGTT ATTTTAAGGA TAGTTCTTGT 
CTCTTGGCAG TATCAATTGG CTGGGAAGGA GGTGTTTATG TACAAACCTG TGGCCACACA 
TTACATATCG ATTGCCATAA ATCTTACATG GAATCATTAC GGAATGACCA GGTTCTTCAG 
GGCTTCTCAG TGGACAAAGG AGAATTCACA TGTCCTCTCT GTAGGCAGTT TGCTAACAGT 
GTTCTTCCTT GTTATCCTGG AAGCAATGTT GAAAACCACC TTTGGCAGCG TCCTAGTAAC 
AAAAGCATAC AAGATCTCAT AAAGGAAGTG GAGGAGCTGC AAGGACGTCC GGGAGCTTTC 
CCATCAGAAA CCAACTTAAG TAAAGAAATG GAATCTGTAA TGAAAGATAT AAAAAATACC 
ACTCAGAAGA AATACAGAGA TTATAGCAAG ACTCCAGGCT CACCAGATAA TGATTTTCTC 
TTTATGTATT CTGTTGCTAG AACCAACTTG GAACTTGAAT TAATTCATCG AGGAGGGAAT 
TTATGTTCAG GTGGTGCAAG TACAGCTGGT AAAAGGTCCT GTTTAAATCA GCTGTTCCAT 
GTATTAGCCT TGCACATGCG ACTTTATAGC ATTGATTCTG AGTATAATCC TTGGAGAAAG 
CTCACACAGT TACTAGAAGA GATGAGTTCG CAACTGGGAA ATGAGGAACA ACAGCCTGAG 
GTTCCAATTC TTTATCATGA TGTTACATCC CTTTTGCTCA TCCAGATCTT AATGATGCCT 
CAACCCTTAC ACAAAGACCA CTTCACCTGC ATTGTGAAGG TGCTTTTTAC CCTACTGTAT 
ACACAAGGTC TTGTGGCACT TTCAGTTAAA TGCAATGAGG AAGATAGGTC AGCCTGGAAA 
CACACAGGAG CCCTCAAAAA GAATACTTGT GATGCAGAAA AGTCTTATGA AGTGTTATTG 
AGCTTTGTGA TAAGTGAACT GTCTAAAGGA AAGTTATACC ATGAAGAAGG AACTCAGGAG 
TGTGCAACGA TTATCCCTGT TGCTTGGTCT CCTGAATCCA TGGAAAAATA CTTACAGGAC 
TTCTGCTTAC CATTTCTCAG AATCACCAGC CTTCTTCAGC ACCACCTTTT TGGGGAAGAT 
TTACCTAGCT GCCAGGAAGA AGAAGAATTT TCAGTTCTTT CCAGCTGCCT GGGACTTCTG 
CCAACATTCT ACCAAACAGA ACATCCATTC ATCAGTGCCT CCTGTCTGGA TTGGCCAGTT 
CCGGCATTTG ATATTATATC TCAGTGGTGT TTTGAGATAA ATTCATTTAC TGAAAGACAT 
GCTGAACAAG GAAAGGCGTT GCTTATCCAA GAGTCAAAAT GGAAATTGCC ACACCTTCTA 
CAGTTGCCTG AAAATTATAA CACCATTTTC CAGTACTACC ACAGAAAAAC CTGTAGTGTC 
TGCACCAAGG TTCCTAAAGA CCCTGCCGTT TGCCTTGTTT GTGGTACTTT TGTATGCCTG 
AAAGGACTTT GCTGCAAGCA ACAAAGTTAC TGTGAATGTG TATTGCATTC TCAGAACTGT 
GGTGCTGGAA CAGGGATTTT CCTTTTGATC AATGCATCAG TGATTATCAT CATTCGAGGC 
CACCGCTTCT GCCTCTGGGG TTCTGTGTAT TTGGATGCTC ATGGAGAGGA AGACCGAGAT 
CTTAGGCGAG GCAAGCCTCT CTACATTTGT AAAGAAAGAT ACAAAGTTCT TGAACAACAG 
TGGATTTCTC ATACTTTTGA TCACATCAAT AAAAGATGGG GTCCACATTA CAATGGGCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR3 ( XM_005202390.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202447.1 Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X2, mRNA.

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CloneID	OBa16371
Clone ID Related Accession (Same CDS sequence)	XM_015462260.1 , XM_005202390.3 , XM_005202390.4
Accession Version	XM_005202390.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase UBR3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202390.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAGTTCA CGTCGGCCTA AAGTTCTTCA AAGAGAGAAT 
TCTGGTACAG CTCAGGTTTT CAGCTTAGTA GCAGAGCGCA GAAAGAAGTT TCAAGAGATC 
ATCAATCGCA GTAACAGTGA AGCAAATCAA GTGGTTCGTC CTAAAACTTC AAGTAAATGG 
TCTGCACCTG GTTCAGCTCC ACAGTTAACT ACAGCCATTT TGGAAATTAA AGAAAGTATA 
TTGTCTTTGT TAATTAAACT TCACCACAAA CTCTCAGGAA AACAAAATTC CTACTATCCT 
CCTTGGCTTG ATGACATAGA AATTTTAATC CAACCAGAAA TTCCTAAATA TAGTCATGGA 
GATGGTATAA CTGCAGTAGA AAGAATTTTA CTAAAAGCTG CATTGCAAAG CAGAATGAAC 
AAACGCATCA TTGAAGAGAT ATGTAGAAAA GTGACCCCTC CTGTACCACC CAAAAAGATT 
ACTGCAGCAG AGAAGAAAAC ACTGGACAAA GAAGAAAGGC GACAAAAAGC TAGAGAAAGA 
CAGCAGAAAT TGCTTGCAGA GTTTGCATCA CGACAGAAAA GCTTCATGGA AACTGCCATG 
GATGTTGATT CTCCAGAGAG TGATATTCCT ATGGAGATCA CCACAGCAGA ACCTCAAGTT 
TCTGAGGCAG TATATGATTG TGTTATTTGT GGACAGAGTG GGCCCTCCTC TGAAGACAGA 
CCTACGGGAT TGGTTGTCCT GTTACAAGCA TCCTCAGTTT TGGGGCAGTG CCGTGACAAC 
ATTGAGCCAA AAAAGTTGCC TATCTCTGAA GAGGAGCAGA TATACCCCTG GGATACATGT 
GCAGCAGTTC ATGACGTGAG GCTCTCATTA TTACAACGTT ATTTTAAGGA TAGTTCTTGT 
CTCTTGGCAG TATCAATTGG CTGGGAAGGA GGTGTTTATG TACAAACCTG TGGCCACACA 
TTACATATCG ATTGCCATAA ATCTTACATG GAATCATTAC GGAATGACCA GGTTCTTCAG 
GGCTTCTCAG TGGACAAAGG AGAATTCACA TGTCCTCTCT GTAGGCAGTT TGCTAACAGT 
GTTCTTCCTT GTTATCCTGG AAGCAATGTT GAAAACCACC TTTGGCAGCG TCCTAGTAAC 
AAAAGCATAC AAGATCTCAT AAAGGAAGTG GAGGAGCTGC AAGGACGTCC GGGAGCTTTC 
CCATCAGAAA CCAACTTAAG TAAAGAAATG GAATCTGTAA TGAAAGATAT AAAAAATACC 
ACTCAGAAGA AATACAGAGA TTATAGCAAG ACTCCAGGCT CACCAGATAA TGATTTTCTC 
TTTATGTATT CTGTTGCTAG AACCAACTTG GAACTTGAAT TAATTCATCG AGGAGGGAAT 
TTATGTTCAG GTGGTGCAAG TACAGCTGGT AAAAGGTCCT GTTTAAATCA GCTGTTCCAT 
GTATTAGCCT TGCACATGCG ACTTTATAGC ATTGATTCTG AGTATAATCC TTGGAGAAAG 
CTCACACAGT TACTAGAAGA GATGAGTTCG CAACTGGGAA ATGAGGAACA ACAGCCTGAG 
GTTCCAATTC TTTATCATGA TGTTACATCC CTTTTGCTCA TCCAGATCTT AATGATGCCT 
CAACCCTTAC ACAAAGACCA CTTCACCTGC ATTGTGAAGG TGCTTTTTAC CCTACTGTAT 
ACACAAGGTC TTGTGGCACT TTCAGTTAAA TGCAATGAGG AAGATAGGTC AGCCTGGAAA 
CACACAGGAG CCCTCAAAAA GAATACTTGT GATGCAGAAA AGTCTTATGA AGTGTTATTG 
AGCTTTGTGA TAAGTGAACT GTCTAAAGGA AAGTTATACC ATGAAGAAGG AACTCAGGAG 
TGTGCAACGA TTATCCCTGT TGCTTGGTCT CCTGAATCCA TGGAAAAATA CTTACAGGAC 
TTCTGCTTAC CATTTCTCAG AATCACCAGC CTTCTTCAGC ACCACCTTTT TGGGGAAGAT 
TTACCTAGCT GCCAGGAAGA AGAAGAATTT TCAGTTCTTT CCAGCTGCCT GGGACTTCTG 
CCAACATTCT ACCAAACAGA ACATCCATTC ATCAGTGCCT CCTGTCTGGA TTGGCCAGTT 
CCGGCATTTG ATATTATATC TCAGTGGTGT TTTGAGATAA ATTCATTTAC TGAAAGACAT 
GCTGAACAAG GAAAGGCGTT GCTTATCCAA GAGTCAAAAT GGAAATTGCC ACACCTTCTA 
CAGTTGCCTG AAAATTATAA CACCATTTTC CAGTACTACC ACAGAAAAAC CTGTAGTGTC 
TGCACCAAGG TTCCTAAAGA CCCTGCCGTT TGCCTTGTTT GTGGTACTTT TGTATGCCTG 
AAAGGACTTT GCTGCAAGCA ACAAAGTTAC TGTGAATGTG TATTGCATTC TCAGAACTGT 
GGTGCTGGAA CAGGGATTTT CCTTTTGATC AATGCATCAG TGATTATCAT CATTCGAGGC 
CACCGCTTCT GCCTCTGGGG TTCTGTGTAT TTGGATGCTC ATGGAGAGGA AGACCGAGAT 
CTTAGGCGAG GCAAGCCTCT CTACATTTGT AAAGAAAGAT ACAAAGTTCT TGAACAACAG 
TGGATTTCTC ATACTTTTGA TCACATCAAT AAAAGATGGG GTCCACATTA CAATGGGCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202447.1
CDS	40..5742
Translation	MAAAAAAAVGGPQPPQAEVPAPGLALDKAATAAHLKAALSRPDNRAGAEELQALLERVLSAERPLAAAADGEEAAAAGGGGGPGAAEEEALEWCKCLLAGGGGYDEFCAAVRAYDPAALCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGDHTGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHQIKSSSNIPCVPKDLLMMSEFVLPRFIFCLIQYLREGYNEPAADVPSEKDLNKVLQLLEPQISFLEDLTKMGGAMRSVLTQVLTNQQNYKDLTSGLGENACARKSHEKYLIALKSSGLTYPEDKLVYGIQEPSAGASTLAVQGFASAAGTLGQLDSSDEEDQDGSQGLGKRKRVKLSSNTKDQSIMDVLKHKSFLEELLFWTIKYEFPQKMVTFLLNMLPDQEYKVAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHGLLVTWMNFVSFFQGMNLNKRELNEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVRETQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAVKCQELDLDSVLPDQEMLMKLMIHPLQIQASLAEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNTVLDAEHERSMLEGALTFLVILLSLRLHLGMSDDEILRAEMVAQLCMNDRTHSSLLDLIPENPNPKSGIIPGSYSFESVLSAVADFKAPVFEPGGSMQQGMYTPKAEVWDHEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGKFPGNPWPPYKKRTPLHPSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNLSEHVLCMVLYLIELGLENSAEEESDEEASACGPERCHDSWFPGSNLVSNMRHFINYVRVRVPETAPEVKRDSLASTSSDSLGSLQSSRRPKVLQRENSGTAQVFSLVAERRKKFQEIINRSNSEANQVVRPKTSSKWSAPGSAPQLTTAILEIKESILSLLIKLHHKLSGKQNSYYPPWLDDIEILIQPEIPKYSHGDGITAVERILLKAALQSRMNKRIIEEICRKVTPPVPPKKITAAEKKTLDKEERRQKARERQQKLLAEFASRQKSFMETAMDVDSPESDIPMEITTAEPQVSEAVYDCVICGQSGPSSEDRPTGLVVLLQASSVLGQCRDNIEPKKLPISEEEQIYPWDTCAAVHDVRLSLLQRYFKDSSCLLAVSIGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCYPGSNVENHLWQRPSNKSIQDLIKEVEELQGRPGAFPSETNLSKEMESVMKDIKNTTQKKYRDYSKTPGSPDNDFLFMYSVARTNLELELIHRGGNLCSGGASTAGKRSCLNQLFHVLALHMRLYSIDSEYNPWRKLTQLLEEMSSQLGNEEQQPEVPILYHDVTSLLLIQILMMPQPLHKDHFTCIVKVLFTLLYTQGLVALSVKCNEEDRSAWKHTGALKKNTCDAEKSYEVLLSFVISELSKGKLYHEEGTQECATIIPVAWSPESMEKYLQDFCLPFLRITSLLQHHLFGEDLPSCQEEEEFSVLSSCLGLLPTFYQTEHPFISASCLDWPVPAFDIISQWCFEINSFTERHAEQGKALLIQESKWKLPHLLQLPENYNTIFQYYHRKTCSVCTKVPKDPAVCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYKVLEQQWISHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_005202390.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202390.3

ORF Insert Sequence:

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAGTTCA CGTCGGCCTA AAGTTCTTCA AAGAGAGAAT 
TCTGGTACAG CTCAGGTTTT CAGCTTAGTA GCAGAGCGCA GAAAGAAGTT TCAAGAGATC 
ATCAATCGCA GTAACAGTGA AGCAAATCAA GTGGTTCGTC CTAAAACTTC AAGTAAATGG 
TCTGCACCTG GTTCAGCTCC ACAGTTAACT ACAGCCATTT TGGAAATTAA AGAAAGTATA 
TTGTCTTTGT TAATTAAACT TCACCACAAA CTCTCAGGAA AACAAAATTC CTACTATCCT 
CCTTGGCTTG ATGACATAGA AATTTTAATC CAACCAGAAA TTCCTAAATA TAGTCATGGA 
GATGGTATAA CTGCAGTAGA AAGAATTTTA CTAAAAGCTG CATTGCAAAG CAGAATGAAC 
AAACGCATCA TTGAAGAGAT ATGTAGAAAA GTGACCCCTC CTGTACCACC CAAAAAGATT 
ACTGCAGCAG AGAAGAAAAC ACTGGACAAA GAAGAAAGGC GACAAAAAGC TAGAGAAAGA 
CAGCAGAAAT TGCTTGCAGA GTTTGCATCA CGACAGAAAA GCTTCATGGA AACTGCCATG 
GATGTTGATT CTCCAGAGAG TGATATTCCT ATGGAGATCA CCACAGCAGA ACCTCAAGTT 
TCTGAGGCAG TATATGATTG TGTTATTTGT GGACAGAGTG GGCCCTCCTC TGAAGACAGA 
CCTACGGGAT TGGTTGTCCT GTTACAAGCA TCCTCAGTTT TGGGGCAGTG CCGTGACAAC 
ATTGAGCCAA AAAAGTTGCC TATCTCTGAA GAGGAGCAGA TATACCCCTG GGATACATGT 
GCAGCAGTTC ATGACGTGAG GCTCTCATTA TTACAACGTT ATTTTAAGGA TAGTTCTTGT 
CTCTTGGCAG TATCAATTGG CTGGGAAGGA GGTGTTTATG TACAAACCTG TGGCCACACA 
TTACATATCG ATTGCCATAA ATCTTACATG GAATCATTAC GGAATGACCA GGTTCTTCAG 
GGCTTCTCAG TGGACAAAGG AGAATTCACA TGTCCTCTCT GTAGGCAGTT TGCTAACAGT 
GTTCTTCCTT GTTATCCTGG AAGCAATGTT GAAAACCACC TTTGGCAGCG TCCTAGTAAC 
AAAAGCATAC AAGATCTCAT AAAGGAAGTG GAGGAGCTGC AAGGACGTCC GGGAGCTTTC 
CCATCAGAAA CCAACTTAAG TAAAGAAATG GAATCTGTAA TGAAAGATAT AAAAAATACC 
ACTCAGAAGA AATACAGAGA TTATAGCAAG ACTCCAGGCT CACCAGATAA TGATTTTCTC 
TTTATGTATT CTGTTGCTAG AACCAACTTG GAACTTGAAT TAATTCATCG AGGAGGGAAT 
TTATGTTCAG GTGGTGCAAG TACAGCTGGT AAAAGGTCCT GTTTAAATCA GCTGTTCCAT 
GTATTAGCCT TGCACATGCG ACTTTATAGC ATTGATTCTG AGTATAATCC TTGGAGAAAG 
CTCACACAGT TACTAGAAGA GATGAGTTCG CAACTGGGAA ATGAGGAACA ACAGCCTGAG 
GTTCCAATTC TTTATCATGA TGTTACATCC CTTTTGCTCA TCCAGATCTT AATGATGCCT 
CAACCCTTAC ACAAAGACCA CTTCACCTGC ATTGTGAAGG TGCTTTTTAC CCTACTGTAT 
ACACAAGGTC TTGTGGCACT TTCAGTTAAA TGCAATGAGG AAGATAGGTC AGCCTGGAAA 
CACACAGGAG CCCTCAAAAA GAATACTTGT GATGCAGAAA AGTCTTATGA AGTGTTATTG 
AGCTTTGTGA TAAGTGAACT GTCTAAAGGA AAGTTATACC ATGAAGAAGG AACTCAGGAG 
TGTGCAACGA TTATCCCTGT TGCTTGGTCT CCTGAATCCA TGGAAAAATA CTTACAGGAC 
TTCTGCTTAC CATTTCTCAG AATCACCAGC CTTCTTCAGC ACCACCTTTT TGGGGAAGAT 
TTACCTAGCT GCCAGGAAGA AGAAGAATTT TCAGTTCTTT CCAGCTGCCT GGGACTTCTG 
CCAACATTCT ACCAAACAGA ACATCCATTC ATCAGTGCCT CCTGTCTGGA TTGGCCAGTT 
CCGGCATTTG ATATTATATC TCAGTGGTGT TTTGAGATAA ATTCATTTAC TGAAAGACAT 
GCTGAACAAG GAAAGGCGTT GCTTATCCAA GAGTCAAAAT GGAAATTGCC ACACCTTCTA 
CAGTTGCCTG AAAATTATAA CACCATTTTC CAGTACTACC ACAGAAAAAC CTGTAGTGTC 
TGCACCAAGG TTCCTAAAGA CCCTGCCGTT TGCCTTGTTT GTGGTACTTT TGTATGCCTG 
AAAGGACTTT GCTGCAAGCA ACAAAGTTAC TGTGAATGTG TATTGCATTC TCAGAACTGT 
GGTGCTGGAA CAGGGATTTT CCTTTTGATC AATGCATCAG TGATTATCAT CATTCGAGGC 
CACCGCTTCT GCCTCTGGGG TTCTGTGTAT TTGGATGCTC ATGGAGAGGA AGACCGAGAT 
CTTAGGCGAG GCAAGCCTCT CTACATTTGT AAAGAAAGAT ACAAAGTTCT TGAACAACAG 
TGGATTTCTC ATACTTTTGA TCACATCAAT AAAAGATGGG GTCCACATTA CAATGGGCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR3 ( XM_005202390.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202447.1 Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa16371
Clone ID Related Accession (Same CDS sequence)	XM_015462260.1 , XM_005202390.3 , XM_005202390.4
Accession Version	XM_005202390.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase UBR3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202390.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAGTTCA CGTCGGCCTA AAGTTCTTCA AAGAGAGAAT 
TCTGGTACAG CTCAGGTTTT CAGCTTAGTA GCAGAGCGCA GAAAGAAGTT TCAAGAGATC 
ATCAATCGCA GTAACAGTGA AGCAAATCAA GTGGTTCGTC CTAAAACTTC AAGTAAATGG 
TCTGCACCTG GTTCAGCTCC ACAGTTAACT ACAGCCATTT TGGAAATTAA AGAAAGTATA 
TTGTCTTTGT TAATTAAACT TCACCACAAA CTCTCAGGAA AACAAAATTC CTACTATCCT 
CCTTGGCTTG ATGACATAGA AATTTTAATC CAACCAGAAA TTCCTAAATA TAGTCATGGA 
GATGGTATAA CTGCAGTAGA AAGAATTTTA CTAAAAGCTG CATTGCAAAG CAGAATGAAC 
AAACGCATCA TTGAAGAGAT ATGTAGAAAA GTGACCCCTC CTGTACCACC CAAAAAGATT 
ACTGCAGCAG AGAAGAAAAC ACTGGACAAA GAAGAAAGGC GACAAAAAGC TAGAGAAAGA 
CAGCAGAAAT TGCTTGCAGA GTTTGCATCA CGACAGAAAA GCTTCATGGA AACTGCCATG 
GATGTTGATT CTCCAGAGAG TGATATTCCT ATGGAGATCA CCACAGCAGA ACCTCAAGTT 
TCTGAGGCAG TATATGATTG TGTTATTTGT GGACAGAGTG GGCCCTCCTC TGAAGACAGA 
CCTACGGGAT TGGTTGTCCT GTTACAAGCA TCCTCAGTTT TGGGGCAGTG CCGTGACAAC 
ATTGAGCCAA AAAAGTTGCC TATCTCTGAA GAGGAGCAGA TATACCCCTG GGATACATGT 
GCAGCAGTTC ATGACGTGAG GCTCTCATTA TTACAACGTT ATTTTAAGGA TAGTTCTTGT 
CTCTTGGCAG TATCAATTGG CTGGGAAGGA GGTGTTTATG TACAAACCTG TGGCCACACA 
TTACATATCG ATTGCCATAA ATCTTACATG GAATCATTAC GGAATGACCA GGTTCTTCAG 
GGCTTCTCAG TGGACAAAGG AGAATTCACA TGTCCTCTCT GTAGGCAGTT TGCTAACAGT 
GTTCTTCCTT GTTATCCTGG AAGCAATGTT GAAAACCACC TTTGGCAGCG TCCTAGTAAC 
AAAAGCATAC AAGATCTCAT AAAGGAAGTG GAGGAGCTGC AAGGACGTCC GGGAGCTTTC 
CCATCAGAAA CCAACTTAAG TAAAGAAATG GAATCTGTAA TGAAAGATAT AAAAAATACC 
ACTCAGAAGA AATACAGAGA TTATAGCAAG ACTCCAGGCT CACCAGATAA TGATTTTCTC 
TTTATGTATT CTGTTGCTAG AACCAACTTG GAACTTGAAT TAATTCATCG AGGAGGGAAT 
TTATGTTCAG GTGGTGCAAG TACAGCTGGT AAAAGGTCCT GTTTAAATCA GCTGTTCCAT 
GTATTAGCCT TGCACATGCG ACTTTATAGC ATTGATTCTG AGTATAATCC TTGGAGAAAG 
CTCACACAGT TACTAGAAGA GATGAGTTCG CAACTGGGAA ATGAGGAACA ACAGCCTGAG 
GTTCCAATTC TTTATCATGA TGTTACATCC CTTTTGCTCA TCCAGATCTT AATGATGCCT 
CAACCCTTAC ACAAAGACCA CTTCACCTGC ATTGTGAAGG TGCTTTTTAC CCTACTGTAT 
ACACAAGGTC TTGTGGCACT TTCAGTTAAA TGCAATGAGG AAGATAGGTC AGCCTGGAAA 
CACACAGGAG CCCTCAAAAA GAATACTTGT GATGCAGAAA AGTCTTATGA AGTGTTATTG 
AGCTTTGTGA TAAGTGAACT GTCTAAAGGA AAGTTATACC ATGAAGAAGG AACTCAGGAG 
TGTGCAACGA TTATCCCTGT TGCTTGGTCT CCTGAATCCA TGGAAAAATA CTTACAGGAC 
TTCTGCTTAC CATTTCTCAG AATCACCAGC CTTCTTCAGC ACCACCTTTT TGGGGAAGAT 
TTACCTAGCT GCCAGGAAGA AGAAGAATTT TCAGTTCTTT CCAGCTGCCT GGGACTTCTG 
CCAACATTCT ACCAAACAGA ACATCCATTC ATCAGTGCCT CCTGTCTGGA TTGGCCAGTT 
CCGGCATTTG ATATTATATC TCAGTGGTGT TTTGAGATAA ATTCATTTAC TGAAAGACAT 
GCTGAACAAG GAAAGGCGTT GCTTATCCAA GAGTCAAAAT GGAAATTGCC ACACCTTCTA 
CAGTTGCCTG AAAATTATAA CACCATTTTC CAGTACTACC ACAGAAAAAC CTGTAGTGTC 
TGCACCAAGG TTCCTAAAGA CCCTGCCGTT TGCCTTGTTT GTGGTACTTT TGTATGCCTG 
AAAGGACTTT GCTGCAAGCA ACAAAGTTAC TGTGAATGTG TATTGCATTC TCAGAACTGT 
GGTGCTGGAA CAGGGATTTT CCTTTTGATC AATGCATCAG TGATTATCAT CATTCGAGGC 
CACCGCTTCT GCCTCTGGGG TTCTGTGTAT TTGGATGCTC ATGGAGAGGA AGACCGAGAT 
CTTAGGCGAG GCAAGCCTCT CTACATTTGT AAAGAAAGAT ACAAAGTTCT TGAACAACAG 
TGGATTTCTC ATACTTTTGA TCACATCAAT AAAAGATGGG GTCCACATTA CAATGGGCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202447.1
CDS	49..5751
Translation	MAAAAAAAVGGPQPPQAEVPAPGLALDKAATAAHLKAALSRPDNRAGAEELQALLERVLSAERPLAAAADGEEAAAAGGGGGPGAAEEEALEWCKCLLAGGGGYDEFCAAVRAYDPAALCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGDHTGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHQIKSSSNIPCVPKDLLMMSEFVLPRFIFCLIQYLREGYNEPAADVPSEKDLNKVLQLLEPQISFLEDLTKMGGAMRSVLTQVLTNQQNYKDLTSGLGENACARKSHEKYLIALKSSGLTYPEDKLVYGIQEPSAGASTLAVQGFASAAGTLGQLDSSDEEDQDGSQGLGKRKRVKLSSNTKDQSIMDVLKHKSFLEELLFWTIKYEFPQKMVTFLLNMLPDQEYKVAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHGLLVTWMNFVSFFQGMNLNKRELNEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVRETQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAVKCQELDLDSVLPDQEMLMKLMIHPLQIQASLAEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNTVLDAEHERSMLEGALTFLVILLSLRLHLGMSDDEILRAEMVAQLCMNDRTHSSLLDLIPENPNPKSGIIPGSYSFESVLSAVADFKAPVFEPGGSMQQGMYTPKAEVWDHEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGKFPGNPWPPYKKRTPLHPSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNLSEHVLCMVLYLIELGLENSAEEESDEEASACGPERCHDSWFPGSNLVSNMRHFINYVRVRVPETAPEVKRDSLASTSSDSLGSLQSSRRPKVLQRENSGTAQVFSLVAERRKKFQEIINRSNSEANQVVRPKTSSKWSAPGSAPQLTTAILEIKESILSLLIKLHHKLSGKQNSYYPPWLDDIEILIQPEIPKYSHGDGITAVERILLKAALQSRMNKRIIEEICRKVTPPVPPKKITAAEKKTLDKEERRQKARERQQKLLAEFASRQKSFMETAMDVDSPESDIPMEITTAEPQVSEAVYDCVICGQSGPSSEDRPTGLVVLLQASSVLGQCRDNIEPKKLPISEEEQIYPWDTCAAVHDVRLSLLQRYFKDSSCLLAVSIGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCYPGSNVENHLWQRPSNKSIQDLIKEVEELQGRPGAFPSETNLSKEMESVMKDIKNTTQKKYRDYSKTPGSPDNDFLFMYSVARTNLELELIHRGGNLCSGGASTAGKRSCLNQLFHVLALHMRLYSIDSEYNPWRKLTQLLEEMSSQLGNEEQQPEVPILYHDVTSLLLIQILMMPQPLHKDHFTCIVKVLFTLLYTQGLVALSVKCNEEDRSAWKHTGALKKNTCDAEKSYEVLLSFVISELSKGKLYHEEGTQECATIIPVAWSPESMEKYLQDFCLPFLRITSLLQHHLFGEDLPSCQEEEEFSVLSSCLGLLPTFYQTEHPFISASCLDWPVPAFDIISQWCFEINSFTERHAEQGKALLIQESKWKLPHLLQLPENYNTIFQYYHRKTCSVCTKVPKDPAVCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYKVLEQQWISHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_005202390.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202390.4

ORF Insert Sequence:

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAGTTCA CGTCGGCCTA AAGTTCTTCA AAGAGAGAAT 
TCTGGTACAG CTCAGGTTTT CAGCTTAGTA GCAGAGCGCA GAAAGAAGTT TCAAGAGATC 
ATCAATCGCA GTAACAGTGA AGCAAATCAA GTGGTTCGTC CTAAAACTTC AAGTAAATGG 
TCTGCACCTG GTTCAGCTCC ACAGTTAACT ACAGCCATTT TGGAAATTAA AGAAAGTATA 
TTGTCTTTGT TAATTAAACT TCACCACAAA CTCTCAGGAA AACAAAATTC CTACTATCCT 
CCTTGGCTTG ATGACATAGA AATTTTAATC CAACCAGAAA TTCCTAAATA TAGTCATGGA 
GATGGTATAA CTGCAGTAGA AAGAATTTTA CTAAAAGCTG CATTGCAAAG CAGAATGAAC 
AAACGCATCA TTGAAGAGAT ATGTAGAAAA GTGACCCCTC CTGTACCACC CAAAAAGATT 
ACTGCAGCAG AGAAGAAAAC ACTGGACAAA GAAGAAAGGC GACAAAAAGC TAGAGAAAGA 
CAGCAGAAAT TGCTTGCAGA GTTTGCATCA CGACAGAAAA GCTTCATGGA AACTGCCATG 
GATGTTGATT CTCCAGAGAG TGATATTCCT ATGGAGATCA CCACAGCAGA ACCTCAAGTT 
TCTGAGGCAG TATATGATTG TGTTATTTGT GGACAGAGTG GGCCCTCCTC TGAAGACAGA 
CCTACGGGAT TGGTTGTCCT GTTACAAGCA TCCTCAGTTT TGGGGCAGTG CCGTGACAAC 
ATTGAGCCAA AAAAGTTGCC TATCTCTGAA GAGGAGCAGA TATACCCCTG GGATACATGT 
GCAGCAGTTC ATGACGTGAG GCTCTCATTA TTACAACGTT ATTTTAAGGA TAGTTCTTGT 
CTCTTGGCAG TATCAATTGG CTGGGAAGGA GGTGTTTATG TACAAACCTG TGGCCACACA 
TTACATATCG ATTGCCATAA ATCTTACATG GAATCATTAC GGAATGACCA GGTTCTTCAG 
GGCTTCTCAG TGGACAAAGG AGAATTCACA TGTCCTCTCT GTAGGCAGTT TGCTAACAGT 
GTTCTTCCTT GTTATCCTGG AAGCAATGTT GAAAACCACC TTTGGCAGCG TCCTAGTAAC 
AAAAGCATAC AAGATCTCAT AAAGGAAGTG GAGGAGCTGC AAGGACGTCC GGGAGCTTTC 
CCATCAGAAA CCAACTTAAG TAAAGAAATG GAATCTGTAA TGAAAGATAT AAAAAATACC 
ACTCAGAAGA AATACAGAGA TTATAGCAAG ACTCCAGGCT CACCAGATAA TGATTTTCTC 
TTTATGTATT CTGTTGCTAG AACCAACTTG GAACTTGAAT TAATTCATCG AGGAGGGAAT 
TTATGTTCAG GTGGTGCAAG TACAGCTGGT AAAAGGTCCT GTTTAAATCA GCTGTTCCAT 
GTATTAGCCT TGCACATGCG ACTTTATAGC ATTGATTCTG AGTATAATCC TTGGAGAAAG 
CTCACACAGT TACTAGAAGA GATGAGTTCG CAACTGGGAA ATGAGGAACA ACAGCCTGAG 
GTTCCAATTC TTTATCATGA TGTTACATCC CTTTTGCTCA TCCAGATCTT AATGATGCCT 
CAACCCTTAC ACAAAGACCA CTTCACCTGC ATTGTGAAGG TGCTTTTTAC CCTACTGTAT 
ACACAAGGTC TTGTGGCACT TTCAGTTAAA TGCAATGAGG AAGATAGGTC AGCCTGGAAA 
CACACAGGAG CCCTCAAAAA GAATACTTGT GATGCAGAAA AGTCTTATGA AGTGTTATTG 
AGCTTTGTGA TAAGTGAACT GTCTAAAGGA AAGTTATACC ATGAAGAAGG AACTCAGGAG 
TGTGCAACGA TTATCCCTGT TGCTTGGTCT CCTGAATCCA TGGAAAAATA CTTACAGGAC 
TTCTGCTTAC CATTTCTCAG AATCACCAGC CTTCTTCAGC ACCACCTTTT TGGGGAAGAT 
TTACCTAGCT GCCAGGAAGA AGAAGAATTT TCAGTTCTTT CCAGCTGCCT GGGACTTCTG 
CCAACATTCT ACCAAACAGA ACATCCATTC ATCAGTGCCT CCTGTCTGGA TTGGCCAGTT 
CCGGCATTTG ATATTATATC TCAGTGGTGT TTTGAGATAA ATTCATTTAC TGAAAGACAT 
GCTGAACAAG GAAAGGCGTT GCTTATCCAA GAGTCAAAAT GGAAATTGCC ACACCTTCTA 
CAGTTGCCTG AAAATTATAA CACCATTTTC CAGTACTACC ACAGAAAAAC CTGTAGTGTC 
TGCACCAAGG TTCCTAAAGA CCCTGCCGTT TGCCTTGTTT GTGGTACTTT TGTATGCCTG 
AAAGGACTTT GCTGCAAGCA ACAAAGTTAC TGTGAATGTG TATTGCATTC TCAGAACTGT 
GGTGCTGGAA CAGGGATTTT CCTTTTGATC AATGCATCAG TGATTATCAT CATTCGAGGC 
CACCGCTTCT GCCTCTGGGG TTCTGTGTAT TTGGATGCTC ATGGAGAGGA AGACCGAGAT 
CTTAGGCGAG GCAAGCCTCT CTACATTTGT AAAGAAAGAT ACAAAGTTCT TGAACAACAG 
TGGATTTCTC ATACTTTTGA TCACATCAAT AAAAGATGGG GTCCACATTA CAATGGGCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR3 ( XM_010801932.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800234.1 Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa53811
Clone ID Related Accession (Same CDS sequence)	XM_010801932.2 , XM_015462259.1 , XM_010801932.3
Accession Version	XM_010801932.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase UBR3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801932.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAGTTCA CGTCGGCCTA AAGTTCTTCA AAGAGAGAAT 
TCTGGTACAG CTCAGGTTTT CAGCTTAGTA GCAGAGCGCA GAAAGAAGTT TCAAGAGATC 
ATCAATCGCA GTAACAGTGA AGCAAATCAA GTGGTTCGTC CTAAAACTTC AAGTAAATGG 
TCTGCACCTG GTTCAGCTCC ACAGTTAACT ACAGCCATTT TGGAAATTAA AGAAAGTATA 
TTGTCTTTGT TAATTAAACT TCACCACAAA CTCTCAGGAA AACAAAATTC CTACTATCCT 
CCTTGGCTTG ATGACATAGA AATTTTAATC CAACCAGAAA TTCCTAAATA TAGTCATGGA 
GATGGTATAA CTGCAGTAGA AAGAATTTTA CTAAAAGCTG CATTGCAAAG CAGAATGAAC 
AAACGCATCA TTGAAGAGAT ATGTAGAAAA GTGACCCCTC CTGTACCACC CAAAAAGATT 
ACTGCAGCAG AGAAGAAAAC ACTGGACAAA GAAGAAAGGC GACAAAAAGC TAGAGAAAGA 
CAGCAGAAAT TGCTTGCAGA GTTTGCATCA CGACAGAAAA GCTTCATGGA AACTGCCATG 
GATGTTGATT CTCCAGAGAG TGATATTCCT ATGGAGATCA CCACAGCAGA ACCTCAAGTT 
TCTGAGGCAG TATATGATTG TGTTATTTGT GGACAGAGTG GGCCCTCCTC TGAAGACAGA 
CCTACGGGAT TGGTTGTCCT GTTACAAGCA TCCTCAGTTT TGGGGCAGTG CCGTGACAAC 
ATTGAGCCAA AAAAGTTGCC TATCTCTGAA GAGGAGCAGA TATACCCCTG GGATACATGT 
GCAGCAGTTC ATGACGTGAG GCTCTCATTA TTACAACGTT ATTTTAAGGA TAGTTCTTGT 
CTCTTGGCAG TATCAATTGG CTGGGAAGGA GGTGTTTATG TACAAACCTG TGGCCACACA 
TTACATATCG ATTGCCATAA ATCTTACATG GAATCATTAC GGAATGACCA GGTTCTTCAG 
GGCTTCTCAG TGGACAAAGG AGAATTCACA TGTCCTCTCT GTAGGCAGTT TGCTAACAGT 
GTTCTTCCTT GTTATCCTGG AAGCAATGTT GAAAACCACC TTTGGCAGCG TCCTAGTAAC 
AAAAGCATAC AAGATCTCAT AAAGGAAGTG GAGGAGCTGC AAGGACGTCC GGGAGCTTTC 
CCAGTAAGCA TCAGTTCAGA AACCAACTTA AGTAAAGAAA TGGAATCTGT AATGAAAGAT 
ATAAAAAATA CCACTCAGAA GAAATACAGA GATTATAGCA AGACTCCAGG CTCACCAGAT 
AATGATTTTC TCTTTATGTA TTCTGTTGCT AGAACCAACT TGGAACTTGA ATTAATTCAT 
CGAGGAGGGA ATTTATGTTC AGGTGGTGCA AGTACAGCTG GTAAAAGGTC CTGTTTAAAT 
CAGCTGTTCC ATGTATTAGC CTTGCACATG CGACTTTATA GCATTGATTC TGAGTATAAT 
CCTTGGAGAA AGCTCACACA GTTACTAGAA GAGATGAGTT CGCAACTGGG AAATGAGGAA 
CAACAGCCTG AGGTTCCAAT TCTTTATCAT GATGTTACAT CCCTTTTGCT CATCCAGATC 
TTAATGATGC CTCAACCCTT ACACAAAGAC CACTTCACCT GCATTGTGAA GGTGCTTTTT 
ACCCTACTGT ATACACAAGG TCTTGTGGCA CTTTCAGTTA AATGCAATGA GGAAGATAGG 
TCAGCCTGGA AACACACAGG AGCCCTCAAA AAGAATACTT GTGATGCAGA AAAGTCTTAT 
GAAGTGTTAT TGAGCTTTGT GATAAGTGAA CTGTCTAAAG GAAAGTTATA CCATGAAGAA 
GGAACTCAGG AGTGTGCAAC GATTATCCCT GTTGCTTGGT CTCCTGAATC CATGGAAAAA 
TACTTACAGG ACTTCTGCTT ACCATTTCTC AGAATCACCA GCCTTCTTCA GCACCACCTT 
TTTGGGGAAG ATTTACCTAG CTGCCAGGAA GAAGAAGAAT TTTCAGTTCT TTCCAGCTGC 
CTGGGACTTC TGCCAACATT CTACCAAACA GAACATCCAT TCATCAGTGC CTCCTGTCTG 
GATTGGCCAG TTCCGGCATT TGATATTATA TCTCAGTGGT GTTTTGAGAT AAATTCATTT 
ACTGAAAGAC ATGCTGAACA AGGAAAGGCG TTGCTTATCC AAGAGTCAAA ATGGAAATTG 
CCACACCTTC TACAGTTGCC TGAAAATTAT AACACCATTT TCCAGTACTA CCACAGAAAA 
ACCTGTAGTG TCTGCACCAA GGTTCCTAAA GACCCTGCCG TTTGCCTTGT TTGTGGTACT 
TTTGTATGCC TGAAAGGACT TTGCTGCAAG CAACAAAGTT ACTGTGAATG TGTATTGCAT 
TCTCAGAACT GTGGTGCTGG AACAGGGATT TTCCTTTTGA TCAATGCATC AGTGATTATC 
ATCATTCGAG GCCACCGCTT CTGCCTCTGG GGTTCTGTGT ATTTGGATGC TCATGGAGAG 
GAAGACCGAG ATCTTAGGCG AGGCAAGCCT CTCTACATTT GTAAAGAAAG ATACAAAGTT 
CTTGAACAAC AGTGGATTTC TCATACTTTT GATCACATCA ATAAAAGATG GGGTCCACAT 
TACAATGGGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800234.1
CDS	41..5755
Translation	MAAAAAAAVGGPQPPQAEVPAPGLALDKAATAAHLKAALSRPDNRAGAEELQALLERVLSAERPLAAAADGEEAAAAGGGGGPGAAEEEALEWCKCLLAGGGGYDEFCAAVRAYDPAALCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGDHTGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHQIKSSSNIPCVPKDLLMMSEFVLPRFIFCLIQYLREGYNEPAADVPSEKDLNKVLQLLEPQISFLEDLTKMGGAMRSVLTQVLTNQQNYKDLTSGLGENACARKSHEKYLIALKSSGLTYPEDKLVYGIQEPSAGASTLAVQGFASAAGTLGQLDSSDEEDQDGSQGLGKRKRVKLSSNTKDQSIMDVLKHKSFLEELLFWTIKYEFPQKMVTFLLNMLPDQEYKVAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHGLLVTWMNFVSFFQGMNLNKRELNEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVRETQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAVKCQELDLDSVLPDQEMLMKLMIHPLQIQASLAEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNTVLDAEHERSMLEGALTFLVILLSLRLHLGMSDDEILRAEMVAQLCMNDRTHSSLLDLIPENPNPKSGIIPGSYSFESVLSAVADFKAPVFEPGGSMQQGMYTPKAEVWDHEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGKFPGNPWPPYKKRTPLHPSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNLSEHVLCMVLYLIELGLENSAEEESDEEASACGPERCHDSWFPGSNLVSNMRHFINYVRVRVPETAPEVKRDSLASTSSDSLGSLQSSRRPKVLQRENSGTAQVFSLVAERRKKFQEIINRSNSEANQVVRPKTSSKWSAPGSAPQLTTAILEIKESILSLLIKLHHKLSGKQNSYYPPWLDDIEILIQPEIPKYSHGDGITAVERILLKAALQSRMNKRIIEEICRKVTPPVPPKKITAAEKKTLDKEERRQKARERQQKLLAEFASRQKSFMETAMDVDSPESDIPMEITTAEPQVSEAVYDCVICGQSGPSSEDRPTGLVVLLQASSVLGQCRDNIEPKKLPISEEEQIYPWDTCAAVHDVRLSLLQRYFKDSSCLLAVSIGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCYPGSNVENHLWQRPSNKSIQDLIKEVEELQGRPGAFPVSISSETNLSKEMESVMKDIKNTTQKKYRDYSKTPGSPDNDFLFMYSVARTNLELELIHRGGNLCSGGASTAGKRSCLNQLFHVLALHMRLYSIDSEYNPWRKLTQLLEEMSSQLGNEEQQPEVPILYHDVTSLLLIQILMMPQPLHKDHFTCIVKVLFTLLYTQGLVALSVKCNEEDRSAWKHTGALKKNTCDAEKSYEVLLSFVISELSKGKLYHEEGTQECATIIPVAWSPESMEKYLQDFCLPFLRITSLLQHHLFGEDLPSCQEEEEFSVLSSCLGLLPTFYQTEHPFISASCLDWPVPAFDIISQWCFEINSFTERHAEQGKALLIQESKWKLPHLLQLPENYNTIFQYYHRKTCSVCTKVPKDPAVCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYKVLEQQWISHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_010801932.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801932.2

ORF Insert Sequence:

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAGTTCA CGTCGGCCTA AAGTTCTTCA AAGAGAGAAT 
TCTGGTACAG CTCAGGTTTT CAGCTTAGTA GCAGAGCGCA GAAAGAAGTT TCAAGAGATC 
ATCAATCGCA GTAACAGTGA AGCAAATCAA GTGGTTCGTC CTAAAACTTC AAGTAAATGG 
TCTGCACCTG GTTCAGCTCC ACAGTTAACT ACAGCCATTT TGGAAATTAA AGAAAGTATA 
TTGTCTTTGT TAATTAAACT TCACCACAAA CTCTCAGGAA AACAAAATTC CTACTATCCT 
CCTTGGCTTG ATGACATAGA AATTTTAATC CAACCAGAAA TTCCTAAATA TAGTCATGGA 
GATGGTATAA CTGCAGTAGA AAGAATTTTA CTAAAAGCTG CATTGCAAAG CAGAATGAAC 
AAACGCATCA TTGAAGAGAT ATGTAGAAAA GTGACCCCTC CTGTACCACC CAAAAAGATT 
ACTGCAGCAG AGAAGAAAAC ACTGGACAAA GAAGAAAGGC GACAAAAAGC TAGAGAAAGA 
CAGCAGAAAT TGCTTGCAGA GTTTGCATCA CGACAGAAAA GCTTCATGGA AACTGCCATG 
GATGTTGATT CTCCAGAGAG TGATATTCCT ATGGAGATCA CCACAGCAGA ACCTCAAGTT 
TCTGAGGCAG TATATGATTG TGTTATTTGT GGACAGAGTG GGCCCTCCTC TGAAGACAGA 
CCTACGGGAT TGGTTGTCCT GTTACAAGCA TCCTCAGTTT TGGGGCAGTG CCGTGACAAC 
ATTGAGCCAA AAAAGTTGCC TATCTCTGAA GAGGAGCAGA TATACCCCTG GGATACATGT 
GCAGCAGTTC ATGACGTGAG GCTCTCATTA TTACAACGTT ATTTTAAGGA TAGTTCTTGT 
CTCTTGGCAG TATCAATTGG CTGGGAAGGA GGTGTTTATG TACAAACCTG TGGCCACACA 
TTACATATCG ATTGCCATAA ATCTTACATG GAATCATTAC GGAATGACCA GGTTCTTCAG 
GGCTTCTCAG TGGACAAAGG AGAATTCACA TGTCCTCTCT GTAGGCAGTT TGCTAACAGT 
GTTCTTCCTT GTTATCCTGG AAGCAATGTT GAAAACCACC TTTGGCAGCG TCCTAGTAAC 
AAAAGCATAC AAGATCTCAT AAAGGAAGTG GAGGAGCTGC AAGGACGTCC GGGAGCTTTC 
CCAGTAAGCA TCAGTTCAGA AACCAACTTA AGTAAAGAAA TGGAATCTGT AATGAAAGAT 
ATAAAAAATA CCACTCAGAA GAAATACAGA GATTATAGCA AGACTCCAGG CTCACCAGAT 
AATGATTTTC TCTTTATGTA TTCTGTTGCT AGAACCAACT TGGAACTTGA ATTAATTCAT 
CGAGGAGGGA ATTTATGTTC AGGTGGTGCA AGTACAGCTG GTAAAAGGTC CTGTTTAAAT 
CAGCTGTTCC ATGTATTAGC CTTGCACATG CGACTTTATA GCATTGATTC TGAGTATAAT 
CCTTGGAGAA AGCTCACACA GTTACTAGAA GAGATGAGTT CGCAACTGGG AAATGAGGAA 
CAACAGCCTG AGGTTCCAAT TCTTTATCAT GATGTTACAT CCCTTTTGCT CATCCAGATC 
TTAATGATGC CTCAACCCTT ACACAAAGAC CACTTCACCT GCATTGTGAA GGTGCTTTTT 
ACCCTACTGT ATACACAAGG TCTTGTGGCA CTTTCAGTTA AATGCAATGA GGAAGATAGG 
TCAGCCTGGA AACACACAGG AGCCCTCAAA AAGAATACTT GTGATGCAGA AAAGTCTTAT 
GAAGTGTTAT TGAGCTTTGT GATAAGTGAA CTGTCTAAAG GAAAGTTATA CCATGAAGAA 
GGAACTCAGG AGTGTGCAAC GATTATCCCT GTTGCTTGGT CTCCTGAATC CATGGAAAAA 
TACTTACAGG ACTTCTGCTT ACCATTTCTC AGAATCACCA GCCTTCTTCA GCACCACCTT 
TTTGGGGAAG ATTTACCTAG CTGCCAGGAA GAAGAAGAAT TTTCAGTTCT TTCCAGCTGC 
CTGGGACTTC TGCCAACATT CTACCAAACA GAACATCCAT TCATCAGTGC CTCCTGTCTG 
GATTGGCCAG TTCCGGCATT TGATATTATA TCTCAGTGGT GTTTTGAGAT AAATTCATTT 
ACTGAAAGAC ATGCTGAACA AGGAAAGGCG TTGCTTATCC AAGAGTCAAA ATGGAAATTG 
CCACACCTTC TACAGTTGCC TGAAAATTAT AACACCATTT TCCAGTACTA CCACAGAAAA 
ACCTGTAGTG TCTGCACCAA GGTTCCTAAA GACCCTGCCG TTTGCCTTGT TTGTGGTACT 
TTTGTATGCC TGAAAGGACT TTGCTGCAAG CAACAAAGTT ACTGTGAATG TGTATTGCAT 
TCTCAGAACT GTGGTGCTGG AACAGGGATT TTCCTTTTGA TCAATGCATC AGTGATTATC 
ATCATTCGAG GCCACCGCTT CTGCCTCTGG GGTTCTGTGT ATTTGGATGC TCATGGAGAG 
GAAGACCGAG ATCTTAGGCG AGGCAAGCCT CTCTACATTT GTAAAGAAAG ATACAAAGTT 
CTTGAACAAC AGTGGATTTC TCATACTTTT GATCACATCA ATAAAAGATG GGGTCCACAT 
TACAATGGGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR3 ( XM_015462259.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317745.1 Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X1, mRNA.

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CloneID	OBa53811
Clone ID Related Accession (Same CDS sequence)	XM_010801932.2 , XM_015462259.1 , XM_010801932.3
Accession Version	XM_015462259.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase UBR3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644584.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAGTTCA CGTCGGCCTA AAGTTCTTCA AAGAGAGAAT 
TCTGGTACAG CTCAGGTTTT CAGCTTAGTA GCAGAGCGCA GAAAGAAGTT TCAAGAGATC 
ATCAATCGCA GTAACAGTGA AGCAAATCAA GTGGTTCGTC CTAAAACTTC AAGTAAATGG 
TCTGCACCTG GTTCAGCTCC ACAGTTAACT ACAGCCATTT TGGAAATTAA AGAAAGTATA 
TTGTCTTTGT TAATTAAACT TCACCACAAA CTCTCAGGAA AACAAAATTC CTACTATCCT 
CCTTGGCTTG ATGACATAGA AATTTTAATC CAACCAGAAA TTCCTAAATA TAGTCATGGA 
GATGGTATAA CTGCAGTAGA AAGAATTTTA CTAAAAGCTG CATTGCAAAG CAGAATGAAC 
AAACGCATCA TTGAAGAGAT ATGTAGAAAA GTGACCCCTC CTGTACCACC CAAAAAGATT 
ACTGCAGCAG AGAAGAAAAC ACTGGACAAA GAAGAAAGGC GACAAAAAGC TAGAGAAAGA 
CAGCAGAAAT TGCTTGCAGA GTTTGCATCA CGACAGAAAA GCTTCATGGA AACTGCCATG 
GATGTTGATT CTCCAGAGAG TGATATTCCT ATGGAGATCA CCACAGCAGA ACCTCAAGTT 
TCTGAGGCAG TATATGATTG TGTTATTTGT GGACAGAGTG GGCCCTCCTC TGAAGACAGA 
CCTACGGGAT TGGTTGTCCT GTTACAAGCA TCCTCAGTTT TGGGGCAGTG CCGTGACAAC 
ATTGAGCCAA AAAAGTTGCC TATCTCTGAA GAGGAGCAGA TATACCCCTG GGATACATGT 
GCAGCAGTTC ATGACGTGAG GCTCTCATTA TTACAACGTT ATTTTAAGGA TAGTTCTTGT 
CTCTTGGCAG TATCAATTGG CTGGGAAGGA GGTGTTTATG TACAAACCTG TGGCCACACA 
TTACATATCG ATTGCCATAA ATCTTACATG GAATCATTAC GGAATGACCA GGTTCTTCAG 
GGCTTCTCAG TGGACAAAGG AGAATTCACA TGTCCTCTCT GTAGGCAGTT TGCTAACAGT 
GTTCTTCCTT GTTATCCTGG AAGCAATGTT GAAAACCACC TTTGGCAGCG TCCTAGTAAC 
AAAAGCATAC AAGATCTCAT AAAGGAAGTG GAGGAGCTGC AAGGACGTCC GGGAGCTTTC 
CCAGTAAGCA TCAGTTCAGA AACCAACTTA AGTAAAGAAA TGGAATCTGT AATGAAAGAT 
ATAAAAAATA CCACTCAGAA GAAATACAGA GATTATAGCA AGACTCCAGG CTCACCAGAT 
AATGATTTTC TCTTTATGTA TTCTGTTGCT AGAACCAACT TGGAACTTGA ATTAATTCAT 
CGAGGAGGGA ATTTATGTTC AGGTGGTGCA AGTACAGCTG GTAAAAGGTC CTGTTTAAAT 
CAGCTGTTCC ATGTATTAGC CTTGCACATG CGACTTTATA GCATTGATTC TGAGTATAAT 
CCTTGGAGAA AGCTCACACA GTTACTAGAA GAGATGAGTT CGCAACTGGG AAATGAGGAA 
CAACAGCCTG AGGTTCCAAT TCTTTATCAT GATGTTACAT CCCTTTTGCT CATCCAGATC 
TTAATGATGC CTCAACCCTT ACACAAAGAC CACTTCACCT GCATTGTGAA GGTGCTTTTT 
ACCCTACTGT ATACACAAGG TCTTGTGGCA CTTTCAGTTA AATGCAATGA GGAAGATAGG 
TCAGCCTGGA AACACACAGG AGCCCTCAAA AAGAATACTT GTGATGCAGA AAAGTCTTAT 
GAAGTGTTAT TGAGCTTTGT GATAAGTGAA CTGTCTAAAG GAAAGTTATA CCATGAAGAA 
GGAACTCAGG AGTGTGCAAC GATTATCCCT GTTGCTTGGT CTCCTGAATC CATGGAAAAA 
TACTTACAGG ACTTCTGCTT ACCATTTCTC AGAATCACCA GCCTTCTTCA GCACCACCTT 
TTTGGGGAAG ATTTACCTAG CTGCCAGGAA GAAGAAGAAT TTTCAGTTCT TTCCAGCTGC 
CTGGGACTTC TGCCAACATT CTACCAAACA GAACATCCAT TCATCAGTGC CTCCTGTCTG 
GATTGGCCAG TTCCGGCATT TGATATTATA TCTCAGTGGT GTTTTGAGAT AAATTCATTT 
ACTGAAAGAC ATGCTGAACA AGGAAAGGCG TTGCTTATCC AAGAGTCAAA ATGGAAATTG 
CCACACCTTC TACAGTTGCC TGAAAATTAT AACACCATTT TCCAGTACTA CCACAGAAAA 
ACCTGTAGTG TCTGCACCAA GGTTCCTAAA GACCCTGCCG TTTGCCTTGT TTGTGGTACT 
TTTGTATGCC TGAAAGGACT TTGCTGCAAG CAACAAAGTT ACTGTGAATG TGTATTGCAT 
TCTCAGAACT GTGGTGCTGG AACAGGGATT TTCCTTTTGA TCAATGCATC AGTGATTATC 
ATCATTCGAG GCCACCGCTT CTGCCTCTGG GGTTCTGTGT ATTTGGATGC TCATGGAGAG 
GAAGACCGAG ATCTTAGGCG AGGCAAGCCT CTCTACATTT GTAAAGAAAG ATACAAAGTT 
CTTGAACAAC AGTGGATTTC TCATACTTTT GATCACATCA ATAAAAGATG GGGTCCACAT 
TACAATGGGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317745.1
CDS	41..5755
Translation	MAAAAAAAVGGPQPPQAEVPAPGLALDKAATAAHLKAALSRPDNRAGAEELQALLERVLSAERPLAAAADGEEAAAAGGGGGPGAAEEEALEWCKCLLAGGGGYDEFCAAVRAYDPAALCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGDHTGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHQIKSSSNIPCVPKDLLMMSEFVLPRFIFCLIQYLREGYNEPAADVPSEKDLNKVLQLLEPQISFLEDLTKMGGAMRSVLTQVLTNQQNYKDLTSGLGENACARKSHEKYLIALKSSGLTYPEDKLVYGIQEPSAGASTLAVQGFASAAGTLGQLDSSDEEDQDGSQGLGKRKRVKLSSNTKDQSIMDVLKHKSFLEELLFWTIKYEFPQKMVTFLLNMLPDQEYKVAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHGLLVTWMNFVSFFQGMNLNKRELNEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVRETQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAVKCQELDLDSVLPDQEMLMKLMIHPLQIQASLAEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNTVLDAEHERSMLEGALTFLVILLSLRLHLGMSDDEILRAEMVAQLCMNDRTHSSLLDLIPENPNPKSGIIPGSYSFESVLSAVADFKAPVFEPGGSMQQGMYTPKAEVWDHEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGKFPGNPWPPYKKRTPLHPSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNLSEHVLCMVLYLIELGLENSAEEESDEEASACGPERCHDSWFPGSNLVSNMRHFINYVRVRVPETAPEVKRDSLASTSSDSLGSLQSSRRPKVLQRENSGTAQVFSLVAERRKKFQEIINRSNSEANQVVRPKTSSKWSAPGSAPQLTTAILEIKESILSLLIKLHHKLSGKQNSYYPPWLDDIEILIQPEIPKYSHGDGITAVERILLKAALQSRMNKRIIEEICRKVTPPVPPKKITAAEKKTLDKEERRQKARERQQKLLAEFASRQKSFMETAMDVDSPESDIPMEITTAEPQVSEAVYDCVICGQSGPSSEDRPTGLVVLLQASSVLGQCRDNIEPKKLPISEEEQIYPWDTCAAVHDVRLSLLQRYFKDSSCLLAVSIGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCYPGSNVENHLWQRPSNKSIQDLIKEVEELQGRPGAFPVSISSETNLSKEMESVMKDIKNTTQKKYRDYSKTPGSPDNDFLFMYSVARTNLELELIHRGGNLCSGGASTAGKRSCLNQLFHVLALHMRLYSIDSEYNPWRKLTQLLEEMSSQLGNEEQQPEVPILYHDVTSLLLIQILMMPQPLHKDHFTCIVKVLFTLLYTQGLVALSVKCNEEDRSAWKHTGALKKNTCDAEKSYEVLLSFVISELSKGKLYHEEGTQECATIIPVAWSPESMEKYLQDFCLPFLRITSLLQHHLFGEDLPSCQEEEEFSVLSSCLGLLPTFYQTEHPFISASCLDWPVPAFDIISQWCFEINSFTERHAEQGKALLIQESKWKLPHLLQLPENYNTIFQYYHRKTCSVCTKVPKDPAVCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYKVLEQQWISHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_015462259.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462259.1

ORF Insert Sequence:

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAGTTCA CGTCGGCCTA AAGTTCTTCA AAGAGAGAAT 
TCTGGTACAG CTCAGGTTTT CAGCTTAGTA GCAGAGCGCA GAAAGAAGTT TCAAGAGATC 
ATCAATCGCA GTAACAGTGA AGCAAATCAA GTGGTTCGTC CTAAAACTTC AAGTAAATGG 
TCTGCACCTG GTTCAGCTCC ACAGTTAACT ACAGCCATTT TGGAAATTAA AGAAAGTATA 
TTGTCTTTGT TAATTAAACT TCACCACAAA CTCTCAGGAA AACAAAATTC CTACTATCCT 
CCTTGGCTTG ATGACATAGA AATTTTAATC CAACCAGAAA TTCCTAAATA TAGTCATGGA 
GATGGTATAA CTGCAGTAGA AAGAATTTTA CTAAAAGCTG CATTGCAAAG CAGAATGAAC 
AAACGCATCA TTGAAGAGAT ATGTAGAAAA GTGACCCCTC CTGTACCACC CAAAAAGATT 
ACTGCAGCAG AGAAGAAAAC ACTGGACAAA GAAGAAAGGC GACAAAAAGC TAGAGAAAGA 
CAGCAGAAAT TGCTTGCAGA GTTTGCATCA CGACAGAAAA GCTTCATGGA AACTGCCATG 
GATGTTGATT CTCCAGAGAG TGATATTCCT ATGGAGATCA CCACAGCAGA ACCTCAAGTT 
TCTGAGGCAG TATATGATTG TGTTATTTGT GGACAGAGTG GGCCCTCCTC TGAAGACAGA 
CCTACGGGAT TGGTTGTCCT GTTACAAGCA TCCTCAGTTT TGGGGCAGTG CCGTGACAAC 
ATTGAGCCAA AAAAGTTGCC TATCTCTGAA GAGGAGCAGA TATACCCCTG GGATACATGT 
GCAGCAGTTC ATGACGTGAG GCTCTCATTA TTACAACGTT ATTTTAAGGA TAGTTCTTGT 
CTCTTGGCAG TATCAATTGG CTGGGAAGGA GGTGTTTATG TACAAACCTG TGGCCACACA 
TTACATATCG ATTGCCATAA ATCTTACATG GAATCATTAC GGAATGACCA GGTTCTTCAG 
GGCTTCTCAG TGGACAAAGG AGAATTCACA TGTCCTCTCT GTAGGCAGTT TGCTAACAGT 
GTTCTTCCTT GTTATCCTGG AAGCAATGTT GAAAACCACC TTTGGCAGCG TCCTAGTAAC 
AAAAGCATAC AAGATCTCAT AAAGGAAGTG GAGGAGCTGC AAGGACGTCC GGGAGCTTTC 
CCAGTAAGCA TCAGTTCAGA AACCAACTTA AGTAAAGAAA TGGAATCTGT AATGAAAGAT 
ATAAAAAATA CCACTCAGAA GAAATACAGA GATTATAGCA AGACTCCAGG CTCACCAGAT 
AATGATTTTC TCTTTATGTA TTCTGTTGCT AGAACCAACT TGGAACTTGA ATTAATTCAT 
CGAGGAGGGA ATTTATGTTC AGGTGGTGCA AGTACAGCTG GTAAAAGGTC CTGTTTAAAT 
CAGCTGTTCC ATGTATTAGC CTTGCACATG CGACTTTATA GCATTGATTC TGAGTATAAT 
CCTTGGAGAA AGCTCACACA GTTACTAGAA GAGATGAGTT CGCAACTGGG AAATGAGGAA 
CAACAGCCTG AGGTTCCAAT TCTTTATCAT GATGTTACAT CCCTTTTGCT CATCCAGATC 
TTAATGATGC CTCAACCCTT ACACAAAGAC CACTTCACCT GCATTGTGAA GGTGCTTTTT 
ACCCTACTGT ATACACAAGG TCTTGTGGCA CTTTCAGTTA AATGCAATGA GGAAGATAGG 
TCAGCCTGGA AACACACAGG AGCCCTCAAA AAGAATACTT GTGATGCAGA AAAGTCTTAT 
GAAGTGTTAT TGAGCTTTGT GATAAGTGAA CTGTCTAAAG GAAAGTTATA CCATGAAGAA 
GGAACTCAGG AGTGTGCAAC GATTATCCCT GTTGCTTGGT CTCCTGAATC CATGGAAAAA 
TACTTACAGG ACTTCTGCTT ACCATTTCTC AGAATCACCA GCCTTCTTCA GCACCACCTT 
TTTGGGGAAG ATTTACCTAG CTGCCAGGAA GAAGAAGAAT TTTCAGTTCT TTCCAGCTGC 
CTGGGACTTC TGCCAACATT CTACCAAACA GAACATCCAT TCATCAGTGC CTCCTGTCTG 
GATTGGCCAG TTCCGGCATT TGATATTATA TCTCAGTGGT GTTTTGAGAT AAATTCATTT 
ACTGAAAGAC ATGCTGAACA AGGAAAGGCG TTGCTTATCC AAGAGTCAAA ATGGAAATTG 
CCACACCTTC TACAGTTGCC TGAAAATTAT AACACCATTT TCCAGTACTA CCACAGAAAA 
ACCTGTAGTG TCTGCACCAA GGTTCCTAAA GACCCTGCCG TTTGCCTTGT TTGTGGTACT 
TTTGTATGCC TGAAAGGACT TTGCTGCAAG CAACAAAGTT ACTGTGAATG TGTATTGCAT 
TCTCAGAACT GTGGTGCTGG AACAGGGATT TTCCTTTTGA TCAATGCATC AGTGATTATC 
ATCATTCGAG GCCACCGCTT CTGCCTCTGG GGTTCTGTGT ATTTGGATGC TCATGGAGAG 
GAAGACCGAG ATCTTAGGCG AGGCAAGCCT CTCTACATTT GTAAAGAAAG ATACAAAGTT 
CTTGAACAAC AGTGGATTTC TCATACTTTT GATCACATCA ATAAAAGATG GGGTCCACAT 
TACAATGGGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR3 ( XM_010801932.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800234.1 Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X1, mRNA.

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CloneID	OBa53811
Clone ID Related Accession (Same CDS sequence)	XM_010801932.2 , XM_015462259.1 , XM_010801932.3
Accession Version	XM_010801932.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase UBR3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801932.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAGTTCA CGTCGGCCTA AAGTTCTTCA AAGAGAGAAT 
TCTGGTACAG CTCAGGTTTT CAGCTTAGTA GCAGAGCGCA GAAAGAAGTT TCAAGAGATC 
ATCAATCGCA GTAACAGTGA AGCAAATCAA GTGGTTCGTC CTAAAACTTC AAGTAAATGG 
TCTGCACCTG GTTCAGCTCC ACAGTTAACT ACAGCCATTT TGGAAATTAA AGAAAGTATA 
TTGTCTTTGT TAATTAAACT TCACCACAAA CTCTCAGGAA AACAAAATTC CTACTATCCT 
CCTTGGCTTG ATGACATAGA AATTTTAATC CAACCAGAAA TTCCTAAATA TAGTCATGGA 
GATGGTATAA CTGCAGTAGA AAGAATTTTA CTAAAAGCTG CATTGCAAAG CAGAATGAAC 
AAACGCATCA TTGAAGAGAT ATGTAGAAAA GTGACCCCTC CTGTACCACC CAAAAAGATT 
ACTGCAGCAG AGAAGAAAAC ACTGGACAAA GAAGAAAGGC GACAAAAAGC TAGAGAAAGA 
CAGCAGAAAT TGCTTGCAGA GTTTGCATCA CGACAGAAAA GCTTCATGGA AACTGCCATG 
GATGTTGATT CTCCAGAGAG TGATATTCCT ATGGAGATCA CCACAGCAGA ACCTCAAGTT 
TCTGAGGCAG TATATGATTG TGTTATTTGT GGACAGAGTG GGCCCTCCTC TGAAGACAGA 
CCTACGGGAT TGGTTGTCCT GTTACAAGCA TCCTCAGTTT TGGGGCAGTG CCGTGACAAC 
ATTGAGCCAA AAAAGTTGCC TATCTCTGAA GAGGAGCAGA TATACCCCTG GGATACATGT 
GCAGCAGTTC ATGACGTGAG GCTCTCATTA TTACAACGTT ATTTTAAGGA TAGTTCTTGT 
CTCTTGGCAG TATCAATTGG CTGGGAAGGA GGTGTTTATG TACAAACCTG TGGCCACACA 
TTACATATCG ATTGCCATAA ATCTTACATG GAATCATTAC GGAATGACCA GGTTCTTCAG 
GGCTTCTCAG TGGACAAAGG AGAATTCACA TGTCCTCTCT GTAGGCAGTT TGCTAACAGT 
GTTCTTCCTT GTTATCCTGG AAGCAATGTT GAAAACCACC TTTGGCAGCG TCCTAGTAAC 
AAAAGCATAC AAGATCTCAT AAAGGAAGTG GAGGAGCTGC AAGGACGTCC GGGAGCTTTC 
CCAGTAAGCA TCAGTTCAGA AACCAACTTA AGTAAAGAAA TGGAATCTGT AATGAAAGAT 
ATAAAAAATA CCACTCAGAA GAAATACAGA GATTATAGCA AGACTCCAGG CTCACCAGAT 
AATGATTTTC TCTTTATGTA TTCTGTTGCT AGAACCAACT TGGAACTTGA ATTAATTCAT 
CGAGGAGGGA ATTTATGTTC AGGTGGTGCA AGTACAGCTG GTAAAAGGTC CTGTTTAAAT 
CAGCTGTTCC ATGTATTAGC CTTGCACATG CGACTTTATA GCATTGATTC TGAGTATAAT 
CCTTGGAGAA AGCTCACACA GTTACTAGAA GAGATGAGTT CGCAACTGGG AAATGAGGAA 
CAACAGCCTG AGGTTCCAAT TCTTTATCAT GATGTTACAT CCCTTTTGCT CATCCAGATC 
TTAATGATGC CTCAACCCTT ACACAAAGAC CACTTCACCT GCATTGTGAA GGTGCTTTTT 
ACCCTACTGT ATACACAAGG TCTTGTGGCA CTTTCAGTTA AATGCAATGA GGAAGATAGG 
TCAGCCTGGA AACACACAGG AGCCCTCAAA AAGAATACTT GTGATGCAGA AAAGTCTTAT 
GAAGTGTTAT TGAGCTTTGT GATAAGTGAA CTGTCTAAAG GAAAGTTATA CCATGAAGAA 
GGAACTCAGG AGTGTGCAAC GATTATCCCT GTTGCTTGGT CTCCTGAATC CATGGAAAAA 
TACTTACAGG ACTTCTGCTT ACCATTTCTC AGAATCACCA GCCTTCTTCA GCACCACCTT 
TTTGGGGAAG ATTTACCTAG CTGCCAGGAA GAAGAAGAAT TTTCAGTTCT TTCCAGCTGC 
CTGGGACTTC TGCCAACATT CTACCAAACA GAACATCCAT TCATCAGTGC CTCCTGTCTG 
GATTGGCCAG TTCCGGCATT TGATATTATA TCTCAGTGGT GTTTTGAGAT AAATTCATTT 
ACTGAAAGAC ATGCTGAACA AGGAAAGGCG TTGCTTATCC AAGAGTCAAA ATGGAAATTG 
CCACACCTTC TACAGTTGCC TGAAAATTAT AACACCATTT TCCAGTACTA CCACAGAAAA 
ACCTGTAGTG TCTGCACCAA GGTTCCTAAA GACCCTGCCG TTTGCCTTGT TTGTGGTACT 
TTTGTATGCC TGAAAGGACT TTGCTGCAAG CAACAAAGTT ACTGTGAATG TGTATTGCAT 
TCTCAGAACT GTGGTGCTGG AACAGGGATT TTCCTTTTGA TCAATGCATC AGTGATTATC 
ATCATTCGAG GCCACCGCTT CTGCCTCTGG GGTTCTGTGT ATTTGGATGC TCATGGAGAG 
GAAGACCGAG ATCTTAGGCG AGGCAAGCCT CTCTACATTT GTAAAGAAAG ATACAAAGTT 
CTTGAACAAC AGTGGATTTC TCATACTTTT GATCACATCA ATAAAAGATG GGGTCCACAT 
TACAATGGGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800234.1
CDS	49..5763
Translation	MAAAAAAAVGGPQPPQAEVPAPGLALDKAATAAHLKAALSRPDNRAGAEELQALLERVLSAERPLAAAADGEEAAAAGGGGGPGAAEEEALEWCKCLLAGGGGYDEFCAAVRAYDPAALCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGDHTGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHQIKSSSNIPCVPKDLLMMSEFVLPRFIFCLIQYLREGYNEPAADVPSEKDLNKVLQLLEPQISFLEDLTKMGGAMRSVLTQVLTNQQNYKDLTSGLGENACARKSHEKYLIALKSSGLTYPEDKLVYGIQEPSAGASTLAVQGFASAAGTLGQLDSSDEEDQDGSQGLGKRKRVKLSSNTKDQSIMDVLKHKSFLEELLFWTIKYEFPQKMVTFLLNMLPDQEYKVAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHGLLVTWMNFVSFFQGMNLNKRELNEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVRETQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAVKCQELDLDSVLPDQEMLMKLMIHPLQIQASLAEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNTVLDAEHERSMLEGALTFLVILLSLRLHLGMSDDEILRAEMVAQLCMNDRTHSSLLDLIPENPNPKSGIIPGSYSFESVLSAVADFKAPVFEPGGSMQQGMYTPKAEVWDHEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGKFPGNPWPPYKKRTPLHPSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNLSEHVLCMVLYLIELGLENSAEEESDEEASACGPERCHDSWFPGSNLVSNMRHFINYVRVRVPETAPEVKRDSLASTSSDSLGSLQSSRRPKVLQRENSGTAQVFSLVAERRKKFQEIINRSNSEANQVVRPKTSSKWSAPGSAPQLTTAILEIKESILSLLIKLHHKLSGKQNSYYPPWLDDIEILIQPEIPKYSHGDGITAVERILLKAALQSRMNKRIIEEICRKVTPPVPPKKITAAEKKTLDKEERRQKARERQQKLLAEFASRQKSFMETAMDVDSPESDIPMEITTAEPQVSEAVYDCVICGQSGPSSEDRPTGLVVLLQASSVLGQCRDNIEPKKLPISEEEQIYPWDTCAAVHDVRLSLLQRYFKDSSCLLAVSIGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCYPGSNVENHLWQRPSNKSIQDLIKEVEELQGRPGAFPVSISSETNLSKEMESVMKDIKNTTQKKYRDYSKTPGSPDNDFLFMYSVARTNLELELIHRGGNLCSGGASTAGKRSCLNQLFHVLALHMRLYSIDSEYNPWRKLTQLLEEMSSQLGNEEQQPEVPILYHDVTSLLLIQILMMPQPLHKDHFTCIVKVLFTLLYTQGLVALSVKCNEEDRSAWKHTGALKKNTCDAEKSYEVLLSFVISELSKGKLYHEEGTQECATIIPVAWSPESMEKYLQDFCLPFLRITSLLQHHLFGEDLPSCQEEEEFSVLSSCLGLLPTFYQTEHPFISASCLDWPVPAFDIISQWCFEINSFTERHAEQGKALLIQESKWKLPHLLQLPENYNTIFQYYHRKTCSVCTKVPKDPAVCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYKVLEQQWISHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_010801932.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801932.3

ORF Insert Sequence:

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAGTTCA CGTCGGCCTA AAGTTCTTCA AAGAGAGAAT 
TCTGGTACAG CTCAGGTTTT CAGCTTAGTA GCAGAGCGCA GAAAGAAGTT TCAAGAGATC 
ATCAATCGCA GTAACAGTGA AGCAAATCAA GTGGTTCGTC CTAAAACTTC AAGTAAATGG 
TCTGCACCTG GTTCAGCTCC ACAGTTAACT ACAGCCATTT TGGAAATTAA AGAAAGTATA 
TTGTCTTTGT TAATTAAACT TCACCACAAA CTCTCAGGAA AACAAAATTC CTACTATCCT 
CCTTGGCTTG ATGACATAGA AATTTTAATC CAACCAGAAA TTCCTAAATA TAGTCATGGA 
GATGGTATAA CTGCAGTAGA AAGAATTTTA CTAAAAGCTG CATTGCAAAG CAGAATGAAC 
AAACGCATCA TTGAAGAGAT ATGTAGAAAA GTGACCCCTC CTGTACCACC CAAAAAGATT 
ACTGCAGCAG AGAAGAAAAC ACTGGACAAA GAAGAAAGGC GACAAAAAGC TAGAGAAAGA 
CAGCAGAAAT TGCTTGCAGA GTTTGCATCA CGACAGAAAA GCTTCATGGA AACTGCCATG 
GATGTTGATT CTCCAGAGAG TGATATTCCT ATGGAGATCA CCACAGCAGA ACCTCAAGTT 
TCTGAGGCAG TATATGATTG TGTTATTTGT GGACAGAGTG GGCCCTCCTC TGAAGACAGA 
CCTACGGGAT TGGTTGTCCT GTTACAAGCA TCCTCAGTTT TGGGGCAGTG CCGTGACAAC 
ATTGAGCCAA AAAAGTTGCC TATCTCTGAA GAGGAGCAGA TATACCCCTG GGATACATGT 
GCAGCAGTTC ATGACGTGAG GCTCTCATTA TTACAACGTT ATTTTAAGGA TAGTTCTTGT 
CTCTTGGCAG TATCAATTGG CTGGGAAGGA GGTGTTTATG TACAAACCTG TGGCCACACA 
TTACATATCG ATTGCCATAA ATCTTACATG GAATCATTAC GGAATGACCA GGTTCTTCAG 
GGCTTCTCAG TGGACAAAGG AGAATTCACA TGTCCTCTCT GTAGGCAGTT TGCTAACAGT 
GTTCTTCCTT GTTATCCTGG AAGCAATGTT GAAAACCACC TTTGGCAGCG TCCTAGTAAC 
AAAAGCATAC AAGATCTCAT AAAGGAAGTG GAGGAGCTGC AAGGACGTCC GGGAGCTTTC 
CCAGTAAGCA TCAGTTCAGA AACCAACTTA AGTAAAGAAA TGGAATCTGT AATGAAAGAT 
ATAAAAAATA CCACTCAGAA GAAATACAGA GATTATAGCA AGACTCCAGG CTCACCAGAT 
AATGATTTTC TCTTTATGTA TTCTGTTGCT AGAACCAACT TGGAACTTGA ATTAATTCAT 
CGAGGAGGGA ATTTATGTTC AGGTGGTGCA AGTACAGCTG GTAAAAGGTC CTGTTTAAAT 
CAGCTGTTCC ATGTATTAGC CTTGCACATG CGACTTTATA GCATTGATTC TGAGTATAAT 
CCTTGGAGAA AGCTCACACA GTTACTAGAA GAGATGAGTT CGCAACTGGG AAATGAGGAA 
CAACAGCCTG AGGTTCCAAT TCTTTATCAT GATGTTACAT CCCTTTTGCT CATCCAGATC 
TTAATGATGC CTCAACCCTT ACACAAAGAC CACTTCACCT GCATTGTGAA GGTGCTTTTT 
ACCCTACTGT ATACACAAGG TCTTGTGGCA CTTTCAGTTA AATGCAATGA GGAAGATAGG 
TCAGCCTGGA AACACACAGG AGCCCTCAAA AAGAATACTT GTGATGCAGA AAAGTCTTAT 
GAAGTGTTAT TGAGCTTTGT GATAAGTGAA CTGTCTAAAG GAAAGTTATA CCATGAAGAA 
GGAACTCAGG AGTGTGCAAC GATTATCCCT GTTGCTTGGT CTCCTGAATC CATGGAAAAA 
TACTTACAGG ACTTCTGCTT ACCATTTCTC AGAATCACCA GCCTTCTTCA GCACCACCTT 
TTTGGGGAAG ATTTACCTAG CTGCCAGGAA GAAGAAGAAT TTTCAGTTCT TTCCAGCTGC 
CTGGGACTTC TGCCAACATT CTACCAAACA GAACATCCAT TCATCAGTGC CTCCTGTCTG 
GATTGGCCAG TTCCGGCATT TGATATTATA TCTCAGTGGT GTTTTGAGAT AAATTCATTT 
ACTGAAAGAC ATGCTGAACA AGGAAAGGCG TTGCTTATCC AAGAGTCAAA ATGGAAATTG 
CCACACCTTC TACAGTTGCC TGAAAATTAT AACACCATTT TCCAGTACTA CCACAGAAAA 
ACCTGTAGTG TCTGCACCAA GGTTCCTAAA GACCCTGCCG TTTGCCTTGT TTGTGGTACT 
TTTGTATGCC TGAAAGGACT TTGCTGCAAG CAACAAAGTT ACTGTGAATG TGTATTGCAT 
TCTCAGAACT GTGGTGCTGG AACAGGGATT TTCCTTTTGA TCAATGCATC AGTGATTATC 
ATCATTCGAG GCCACCGCTT CTGCCTCTGG GGTTCTGTGT ATTTGGATGC TCATGGAGAG 
GAAGACCGAG ATCTTAGGCG AGGCAAGCCT CTCTACATTT GTAAAGAAAG ATACAAAGTT 
CTTGAACAAC AGTGGATTTC TCATACTTTT GATCACATCA ATAAAAGATG GGGTCCACAT 
TACAATGGGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR3 ( XM_015462261.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317747.1 Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X3, mRNA.

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CloneID	OBa53812
Clone ID Related Accession (Same CDS sequence)	XM_015462261.1 , XM_010801933.3 , XM_010801933.2
Accession Version	XM_015462261.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase UBR3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644584.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAATTCT GGTACAGCTC AGGTTTTCAG CTTAGTAGCA 
GAGCGCAGAA AGAAGTTTCA AGAGATCATC AATCGCAGTA ACAGTGAAGC AAATCAAGTG 
GTTCGTCCTA AAACTTCAAG TAAATGGTCT GCACCTGGTT CAGCTCCACA GTTAACTACA 
GCCATTTTGG AAATTAAAGA AAGTATATTG TCTTTGTTAA TTAAACTTCA CCACAAACTC 
TCAGGAAAAC AAAATTCCTA CTATCCTCCT TGGCTTGATG ACATAGAAAT TTTAATCCAA 
CCAGAAATTC CTAAATATAG TCATGGAGAT GGTATAACTG CAGTAGAAAG AATTTTACTA 
AAAGCTGCAT TGCAAAGCAG AATGAACAAA CGCATCATTG AAGAGATATG TAGAAAAGTG 
ACCCCTCCTG TACCACCCAA AAAGATTACT GCAGCAGAGA AGAAAACACT GGACAAAGAA 
GAAAGGCGAC AAAAAGCTAG AGAAAGACAG CAGAAATTGC TTGCAGAGTT TGCATCACGA 
CAGAAAAGCT TCATGGAAAC TGCCATGGAT GTTGATTCTC CAGAGAGTGA TATTCCTATG 
GAGATCACCA CAGCAGAACC TCAAGTTTCT GAGGCAGTAT ATGATTGTGT TATTTGTGGA 
CAGAGTGGGC CCTCCTCTGA AGACAGACCT ACGGGATTGG TTGTCCTGTT ACAAGCATCC 
TCAGTTTTGG GGCAGTGCCG TGACAACATT GAGCCAAAAA AGTTGCCTAT CTCTGAAGAG 
GAGCAGATAT ACCCCTGGGA TACATGTGCA GCAGTTCATG ACGTGAGGCT CTCATTATTA 
CAACGTTATT TTAAGGATAG TTCTTGTCTC TTGGCAGTAT CAATTGGCTG GGAAGGAGGT 
GTTTATGTAC AAACCTGTGG CCACACATTA CATATCGATT GCCATAAATC TTACATGGAA 
TCATTACGGA ATGACCAGGT TCTTCAGGGC TTCTCAGTGG ACAAAGGAGA ATTCACATGT 
CCTCTCTGTA GGCAGTTTGC TAACAGTGTT CTTCCTTGTT ATCCTGGAAG CAATGTTGAA 
AACCACCTTT GGCAGCGTCC TAGTAACAAA AGCATACAAG ATCTCATAAA GGAAGTGGAG 
GAGCTGCAAG GACGTCCGGG AGCTTTCCCA GTAAGCATCA GTTCAGAAAC CAACTTAAGT 
AAAGAAATGG AATCTGTAAT GAAAGATATA AAAAATACCA CTCAGAAGAA ATACAGAGAT 
TATAGCAAGA CTCCAGGCTC ACCAGATAAT GATTTTCTCT TTATGTATTC TGTTGCTAGA 
ACCAACTTGG AACTTGAATT AATTCATCGA GGAGGGAATT TATGTTCAGG TGGTGCAAGT 
ACAGCTGGTA AAAGGTCCTG TTTAAATCAG CTGTTCCATG TATTAGCCTT GCACATGCGA 
CTTTATAGCA TTGATTCTGA GTATAATCCT TGGAGAAAGC TCACACAGTT ACTAGAAGAG 
ATGAGTTCGC AACTGGGAAA TGAGGAACAA CAGCCTGAGG TTCCAATTCT TTATCATGAT 
GTTACATCCC TTTTGCTCAT CCAGATCTTA ATGATGCCTC AACCCTTACA CAAAGACCAC 
TTCACCTGCA TTGTGAAGGT GCTTTTTACC CTACTGTATA CACAAGGTCT TGTGGCACTT 
TCAGTTAAAT GCAATGAGGA AGATAGGTCA GCCTGGAAAC ACACAGGAGC CCTCAAAAAG 
AATACTTGTG ATGCAGAAAA GTCTTATGAA GTGTTATTGA GCTTTGTGAT AAGTGAACTG 
TCTAAAGGAA AGTTATACCA TGAAGAAGGA ACTCAGGAGT GTGCAACGAT TATCCCTGTT 
GCTTGGTCTC CTGAATCCAT GGAAAAATAC TTACAGGACT TCTGCTTACC ATTTCTCAGA 
ATCACCAGCC TTCTTCAGCA CCACCTTTTT GGGGAAGATT TACCTAGCTG CCAGGAAGAA 
GAAGAATTTT CAGTTCTTTC CAGCTGCCTG GGACTTCTGC CAACATTCTA CCAAACAGAA 
CATCCATTCA TCAGTGCCTC CTGTCTGGAT TGGCCAGTTC CGGCATTTGA TATTATATCT 
CAGTGGTGTT TTGAGATAAA TTCATTTACT GAAAGACATG CTGAACAAGG AAAGGCGTTG 
CTTATCCAAG AGTCAAAATG GAAATTGCCA CACCTTCTAC AGTTGCCTGA AAATTATAAC 
ACCATTTTCC AGTACTACCA CAGAAAAACC TGTAGTGTCT GCACCAAGGT TCCTAAAGAC 
CCTGCCGTTT GCCTTGTTTG TGGTACTTTT GTATGCCTGA AAGGACTTTG CTGCAAGCAA 
CAAAGTTACT GTGAATGTGT ATTGCATTCT CAGAACTGTG GTGCTGGAAC AGGGATTTTC 
CTTTTGATCA ATGCATCAGT GATTATCATC ATTCGAGGCC ACCGCTTCTG CCTCTGGGGT 
TCTGTGTATT TGGATGCTCA TGGAGAGGAA GACCGAGATC TTAGGCGAGG CAAGCCTCTC 
TACATTTGTA AAGAAAGATA CAAAGTTCTT GAACAACAGT GGATTTCTCA TACTTTTGAT 
CACATCAATA AAAGATGGGG TCCACATTAC AATGGGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317747.1
CDS	40..5721
Translation	MAAAAAAAVGGPQPPQAEVPAPGLALDKAATAAHLKAALSRPDNRAGAEELQALLERVLSAERPLAAAADGEEAAAAGGGGGPGAAEEEALEWCKCLLAGGGGYDEFCAAVRAYDPAALCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGDHTGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHQIKSSSNIPCVPKDLLMMSEFVLPRFIFCLIQYLREGYNEPAADVPSEKDLNKVLQLLEPQISFLEDLTKMGGAMRSVLTQVLTNQQNYKDLTSGLGENACARKSHEKYLIALKSSGLTYPEDKLVYGIQEPSAGASTLAVQGFASAAGTLGQLDSSDEEDQDGSQGLGKRKRVKLSSNTKDQSIMDVLKHKSFLEELLFWTIKYEFPQKMVTFLLNMLPDQEYKVAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHGLLVTWMNFVSFFQGMNLNKRELNEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVRETQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAVKCQELDLDSVLPDQEMLMKLMIHPLQIQASLAEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNTVLDAEHERSMLEGALTFLVILLSLRLHLGMSDDEILRAEMVAQLCMNDRTHSSLLDLIPENPNPKSGIIPGSYSFESVLSAVADFKAPVFEPGGSMQQGMYTPKAEVWDHEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGKFPGNPWPPYKKRTPLHPSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNLSEHVLCMVLYLIELGLENSAEEESDEEASACGPERCHDSWFPGSNLVSNMRHFINYVRVRVPETAPEVKRDSLASTSSDSLGSLQNSGTAQVFSLVAERRKKFQEIINRSNSEANQVVRPKTSSKWSAPGSAPQLTTAILEIKESILSLLIKLHHKLSGKQNSYYPPWLDDIEILIQPEIPKYSHGDGITAVERILLKAALQSRMNKRIIEEICRKVTPPVPPKKITAAEKKTLDKEERRQKARERQQKLLAEFASRQKSFMETAMDVDSPESDIPMEITTAEPQVSEAVYDCVICGQSGPSSEDRPTGLVVLLQASSVLGQCRDNIEPKKLPISEEEQIYPWDTCAAVHDVRLSLLQRYFKDSSCLLAVSIGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCYPGSNVENHLWQRPSNKSIQDLIKEVEELQGRPGAFPVSISSETNLSKEMESVMKDIKNTTQKKYRDYSKTPGSPDNDFLFMYSVARTNLELELIHRGGNLCSGGASTAGKRSCLNQLFHVLALHMRLYSIDSEYNPWRKLTQLLEEMSSQLGNEEQQPEVPILYHDVTSLLLIQILMMPQPLHKDHFTCIVKVLFTLLYTQGLVALSVKCNEEDRSAWKHTGALKKNTCDAEKSYEVLLSFVISELSKGKLYHEEGTQECATIIPVAWSPESMEKYLQDFCLPFLRITSLLQHHLFGEDLPSCQEEEEFSVLSSCLGLLPTFYQTEHPFISASCLDWPVPAFDIISQWCFEINSFTERHAEQGKALLIQESKWKLPHLLQLPENYNTIFQYYHRKTCSVCTKVPKDPAVCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYKVLEQQWISHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_015462261.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462261.1

ORF Insert Sequence:

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAATTCT GGTACAGCTC AGGTTTTCAG CTTAGTAGCA 
GAGCGCAGAA AGAAGTTTCA AGAGATCATC AATCGCAGTA ACAGTGAAGC AAATCAAGTG 
GTTCGTCCTA AAACTTCAAG TAAATGGTCT GCACCTGGTT CAGCTCCACA GTTAACTACA 
GCCATTTTGG AAATTAAAGA AAGTATATTG TCTTTGTTAA TTAAACTTCA CCACAAACTC 
TCAGGAAAAC AAAATTCCTA CTATCCTCCT TGGCTTGATG ACATAGAAAT TTTAATCCAA 
CCAGAAATTC CTAAATATAG TCATGGAGAT GGTATAACTG CAGTAGAAAG AATTTTACTA 
AAAGCTGCAT TGCAAAGCAG AATGAACAAA CGCATCATTG AAGAGATATG TAGAAAAGTG 
ACCCCTCCTG TACCACCCAA AAAGATTACT GCAGCAGAGA AGAAAACACT GGACAAAGAA 
GAAAGGCGAC AAAAAGCTAG AGAAAGACAG CAGAAATTGC TTGCAGAGTT TGCATCACGA 
CAGAAAAGCT TCATGGAAAC TGCCATGGAT GTTGATTCTC CAGAGAGTGA TATTCCTATG 
GAGATCACCA CAGCAGAACC TCAAGTTTCT GAGGCAGTAT ATGATTGTGT TATTTGTGGA 
CAGAGTGGGC CCTCCTCTGA AGACAGACCT ACGGGATTGG TTGTCCTGTT ACAAGCATCC 
TCAGTTTTGG GGCAGTGCCG TGACAACATT GAGCCAAAAA AGTTGCCTAT CTCTGAAGAG 
GAGCAGATAT ACCCCTGGGA TACATGTGCA GCAGTTCATG ACGTGAGGCT CTCATTATTA 
CAACGTTATT TTAAGGATAG TTCTTGTCTC TTGGCAGTAT CAATTGGCTG GGAAGGAGGT 
GTTTATGTAC AAACCTGTGG CCACACATTA CATATCGATT GCCATAAATC TTACATGGAA 
TCATTACGGA ATGACCAGGT TCTTCAGGGC TTCTCAGTGG ACAAAGGAGA ATTCACATGT 
CCTCTCTGTA GGCAGTTTGC TAACAGTGTT CTTCCTTGTT ATCCTGGAAG CAATGTTGAA 
AACCACCTTT GGCAGCGTCC TAGTAACAAA AGCATACAAG ATCTCATAAA GGAAGTGGAG 
GAGCTGCAAG GACGTCCGGG AGCTTTCCCA GTAAGCATCA GTTCAGAAAC CAACTTAAGT 
AAAGAAATGG AATCTGTAAT GAAAGATATA AAAAATACCA CTCAGAAGAA ATACAGAGAT 
TATAGCAAGA CTCCAGGCTC ACCAGATAAT GATTTTCTCT TTATGTATTC TGTTGCTAGA 
ACCAACTTGG AACTTGAATT AATTCATCGA GGAGGGAATT TATGTTCAGG TGGTGCAAGT 
ACAGCTGGTA AAAGGTCCTG TTTAAATCAG CTGTTCCATG TATTAGCCTT GCACATGCGA 
CTTTATAGCA TTGATTCTGA GTATAATCCT TGGAGAAAGC TCACACAGTT ACTAGAAGAG 
ATGAGTTCGC AACTGGGAAA TGAGGAACAA CAGCCTGAGG TTCCAATTCT TTATCATGAT 
GTTACATCCC TTTTGCTCAT CCAGATCTTA ATGATGCCTC AACCCTTACA CAAAGACCAC 
TTCACCTGCA TTGTGAAGGT GCTTTTTACC CTACTGTATA CACAAGGTCT TGTGGCACTT 
TCAGTTAAAT GCAATGAGGA AGATAGGTCA GCCTGGAAAC ACACAGGAGC CCTCAAAAAG 
AATACTTGTG ATGCAGAAAA GTCTTATGAA GTGTTATTGA GCTTTGTGAT AAGTGAACTG 
TCTAAAGGAA AGTTATACCA TGAAGAAGGA ACTCAGGAGT GTGCAACGAT TATCCCTGTT 
GCTTGGTCTC CTGAATCCAT GGAAAAATAC TTACAGGACT TCTGCTTACC ATTTCTCAGA 
ATCACCAGCC TTCTTCAGCA CCACCTTTTT GGGGAAGATT TACCTAGCTG CCAGGAAGAA 
GAAGAATTTT CAGTTCTTTC CAGCTGCCTG GGACTTCTGC CAACATTCTA CCAAACAGAA 
CATCCATTCA TCAGTGCCTC CTGTCTGGAT TGGCCAGTTC CGGCATTTGA TATTATATCT 
CAGTGGTGTT TTGAGATAAA TTCATTTACT GAAAGACATG CTGAACAAGG AAAGGCGTTG 
CTTATCCAAG AGTCAAAATG GAAATTGCCA CACCTTCTAC AGTTGCCTGA AAATTATAAC 
ACCATTTTCC AGTACTACCA CAGAAAAACC TGTAGTGTCT GCACCAAGGT TCCTAAAGAC 
CCTGCCGTTT GCCTTGTTTG TGGTACTTTT GTATGCCTGA AAGGACTTTG CTGCAAGCAA 
CAAAGTTACT GTGAATGTGT ATTGCATTCT CAGAACTGTG GTGCTGGAAC AGGGATTTTC 
CTTTTGATCA ATGCATCAGT GATTATCATC ATTCGAGGCC ACCGCTTCTG CCTCTGGGGT 
TCTGTGTATT TGGATGCTCA TGGAGAGGAA GACCGAGATC TTAGGCGAGG CAAGCCTCTC 
TACATTTGTA AAGAAAGATA CAAAGTTCTT GAACAACAGT GGATTTCTCA TACTTTTGAT 
CACATCAATA AAAGATGGGG TCCACATTAC AATGGGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR3 ( XM_010801933.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800235.1 Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X4, mRNA.

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CloneID	OBa53812
Clone ID Related Accession (Same CDS sequence)	XM_015462261.1 , XM_010801933.3 , XM_010801933.2
Accession Version	XM_010801933.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase UBR3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801933.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAATTCT GGTACAGCTC AGGTTTTCAG CTTAGTAGCA 
GAGCGCAGAA AGAAGTTTCA AGAGATCATC AATCGCAGTA ACAGTGAAGC AAATCAAGTG 
GTTCGTCCTA AAACTTCAAG TAAATGGTCT GCACCTGGTT CAGCTCCACA GTTAACTACA 
GCCATTTTGG AAATTAAAGA AAGTATATTG TCTTTGTTAA TTAAACTTCA CCACAAACTC 
TCAGGAAAAC AAAATTCCTA CTATCCTCCT TGGCTTGATG ACATAGAAAT TTTAATCCAA 
CCAGAAATTC CTAAATATAG TCATGGAGAT GGTATAACTG CAGTAGAAAG AATTTTACTA 
AAAGCTGCAT TGCAAAGCAG AATGAACAAA CGCATCATTG AAGAGATATG TAGAAAAGTG 
ACCCCTCCTG TACCACCCAA AAAGATTACT GCAGCAGAGA AGAAAACACT GGACAAAGAA 
GAAAGGCGAC AAAAAGCTAG AGAAAGACAG CAGAAATTGC TTGCAGAGTT TGCATCACGA 
CAGAAAAGCT TCATGGAAAC TGCCATGGAT GTTGATTCTC CAGAGAGTGA TATTCCTATG 
GAGATCACCA CAGCAGAACC TCAAGTTTCT GAGGCAGTAT ATGATTGTGT TATTTGTGGA 
CAGAGTGGGC CCTCCTCTGA AGACAGACCT ACGGGATTGG TTGTCCTGTT ACAAGCATCC 
TCAGTTTTGG GGCAGTGCCG TGACAACATT GAGCCAAAAA AGTTGCCTAT CTCTGAAGAG 
GAGCAGATAT ACCCCTGGGA TACATGTGCA GCAGTTCATG ACGTGAGGCT CTCATTATTA 
CAACGTTATT TTAAGGATAG TTCTTGTCTC TTGGCAGTAT CAATTGGCTG GGAAGGAGGT 
GTTTATGTAC AAACCTGTGG CCACACATTA CATATCGATT GCCATAAATC TTACATGGAA 
TCATTACGGA ATGACCAGGT TCTTCAGGGC TTCTCAGTGG ACAAAGGAGA ATTCACATGT 
CCTCTCTGTA GGCAGTTTGC TAACAGTGTT CTTCCTTGTT ATCCTGGAAG CAATGTTGAA 
AACCACCTTT GGCAGCGTCC TAGTAACAAA AGCATACAAG ATCTCATAAA GGAAGTGGAG 
GAGCTGCAAG GACGTCCGGG AGCTTTCCCA GTAAGCATCA GTTCAGAAAC CAACTTAAGT 
AAAGAAATGG AATCTGTAAT GAAAGATATA AAAAATACCA CTCAGAAGAA ATACAGAGAT 
TATAGCAAGA CTCCAGGCTC ACCAGATAAT GATTTTCTCT TTATGTATTC TGTTGCTAGA 
ACCAACTTGG AACTTGAATT AATTCATCGA GGAGGGAATT TATGTTCAGG TGGTGCAAGT 
ACAGCTGGTA AAAGGTCCTG TTTAAATCAG CTGTTCCATG TATTAGCCTT GCACATGCGA 
CTTTATAGCA TTGATTCTGA GTATAATCCT TGGAGAAAGC TCACACAGTT ACTAGAAGAG 
ATGAGTTCGC AACTGGGAAA TGAGGAACAA CAGCCTGAGG TTCCAATTCT TTATCATGAT 
GTTACATCCC TTTTGCTCAT CCAGATCTTA ATGATGCCTC AACCCTTACA CAAAGACCAC 
TTCACCTGCA TTGTGAAGGT GCTTTTTACC CTACTGTATA CACAAGGTCT TGTGGCACTT 
TCAGTTAAAT GCAATGAGGA AGATAGGTCA GCCTGGAAAC ACACAGGAGC CCTCAAAAAG 
AATACTTGTG ATGCAGAAAA GTCTTATGAA GTGTTATTGA GCTTTGTGAT AAGTGAACTG 
TCTAAAGGAA AGTTATACCA TGAAGAAGGA ACTCAGGAGT GTGCAACGAT TATCCCTGTT 
GCTTGGTCTC CTGAATCCAT GGAAAAATAC TTACAGGACT TCTGCTTACC ATTTCTCAGA 
ATCACCAGCC TTCTTCAGCA CCACCTTTTT GGGGAAGATT TACCTAGCTG CCAGGAAGAA 
GAAGAATTTT CAGTTCTTTC CAGCTGCCTG GGACTTCTGC CAACATTCTA CCAAACAGAA 
CATCCATTCA TCAGTGCCTC CTGTCTGGAT TGGCCAGTTC CGGCATTTGA TATTATATCT 
CAGTGGTGTT TTGAGATAAA TTCATTTACT GAAAGACATG CTGAACAAGG AAAGGCGTTG 
CTTATCCAAG AGTCAAAATG GAAATTGCCA CACCTTCTAC AGTTGCCTGA AAATTATAAC 
ACCATTTTCC AGTACTACCA CAGAAAAACC TGTAGTGTCT GCACCAAGGT TCCTAAAGAC 
CCTGCCGTTT GCCTTGTTTG TGGTACTTTT GTATGCCTGA AAGGACTTTG CTGCAAGCAA 
CAAAGTTACT GTGAATGTGT ATTGCATTCT CAGAACTGTG GTGCTGGAAC AGGGATTTTC 
CTTTTGATCA ATGCATCAGT GATTATCATC ATTCGAGGCC ACCGCTTCTG CCTCTGGGGT 
TCTGTGTATT TGGATGCTCA TGGAGAGGAA GACCGAGATC TTAGGCGAGG CAAGCCTCTC 
TACATTTGTA AAGAAAGATA CAAAGTTCTT GAACAACAGT GGATTTCTCA TACTTTTGAT 
CACATCAATA AAAGATGGGG TCCACATTAC AATGGGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800235.1
CDS	49..5730
Translation	MAAAAAAAVGGPQPPQAEVPAPGLALDKAATAAHLKAALSRPDNRAGAEELQALLERVLSAERPLAAAADGEEAAAAGGGGGPGAAEEEALEWCKCLLAGGGGYDEFCAAVRAYDPAALCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGDHTGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHQIKSSSNIPCVPKDLLMMSEFVLPRFIFCLIQYLREGYNEPAADVPSEKDLNKVLQLLEPQISFLEDLTKMGGAMRSVLTQVLTNQQNYKDLTSGLGENACARKSHEKYLIALKSSGLTYPEDKLVYGIQEPSAGASTLAVQGFASAAGTLGQLDSSDEEDQDGSQGLGKRKRVKLSSNTKDQSIMDVLKHKSFLEELLFWTIKYEFPQKMVTFLLNMLPDQEYKVAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHGLLVTWMNFVSFFQGMNLNKRELNEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVRETQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAVKCQELDLDSVLPDQEMLMKLMIHPLQIQASLAEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNTVLDAEHERSMLEGALTFLVILLSLRLHLGMSDDEILRAEMVAQLCMNDRTHSSLLDLIPENPNPKSGIIPGSYSFESVLSAVADFKAPVFEPGGSMQQGMYTPKAEVWDHEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGKFPGNPWPPYKKRTPLHPSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNLSEHVLCMVLYLIELGLENSAEEESDEEASACGPERCHDSWFPGSNLVSNMRHFINYVRVRVPETAPEVKRDSLASTSSDSLGSLQNSGTAQVFSLVAERRKKFQEIINRSNSEANQVVRPKTSSKWSAPGSAPQLTTAILEIKESILSLLIKLHHKLSGKQNSYYPPWLDDIEILIQPEIPKYSHGDGITAVERILLKAALQSRMNKRIIEEICRKVTPPVPPKKITAAEKKTLDKEERRQKARERQQKLLAEFASRQKSFMETAMDVDSPESDIPMEITTAEPQVSEAVYDCVICGQSGPSSEDRPTGLVVLLQASSVLGQCRDNIEPKKLPISEEEQIYPWDTCAAVHDVRLSLLQRYFKDSSCLLAVSIGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCYPGSNVENHLWQRPSNKSIQDLIKEVEELQGRPGAFPVSISSETNLSKEMESVMKDIKNTTQKKYRDYSKTPGSPDNDFLFMYSVARTNLELELIHRGGNLCSGGASTAGKRSCLNQLFHVLALHMRLYSIDSEYNPWRKLTQLLEEMSSQLGNEEQQPEVPILYHDVTSLLLIQILMMPQPLHKDHFTCIVKVLFTLLYTQGLVALSVKCNEEDRSAWKHTGALKKNTCDAEKSYEVLLSFVISELSKGKLYHEEGTQECATIIPVAWSPESMEKYLQDFCLPFLRITSLLQHHLFGEDLPSCQEEEEFSVLSSCLGLLPTFYQTEHPFISASCLDWPVPAFDIISQWCFEINSFTERHAEQGKALLIQESKWKLPHLLQLPENYNTIFQYYHRKTCSVCTKVPKDPAVCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYKVLEQQWISHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_010801933.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801933.3

ORF Insert Sequence:

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAATTCT GGTACAGCTC AGGTTTTCAG CTTAGTAGCA 
GAGCGCAGAA AGAAGTTTCA AGAGATCATC AATCGCAGTA ACAGTGAAGC AAATCAAGTG 
GTTCGTCCTA AAACTTCAAG TAAATGGTCT GCACCTGGTT CAGCTCCACA GTTAACTACA 
GCCATTTTGG AAATTAAAGA AAGTATATTG TCTTTGTTAA TTAAACTTCA CCACAAACTC 
TCAGGAAAAC AAAATTCCTA CTATCCTCCT TGGCTTGATG ACATAGAAAT TTTAATCCAA 
CCAGAAATTC CTAAATATAG TCATGGAGAT GGTATAACTG CAGTAGAAAG AATTTTACTA 
AAAGCTGCAT TGCAAAGCAG AATGAACAAA CGCATCATTG AAGAGATATG TAGAAAAGTG 
ACCCCTCCTG TACCACCCAA AAAGATTACT GCAGCAGAGA AGAAAACACT GGACAAAGAA 
GAAAGGCGAC AAAAAGCTAG AGAAAGACAG CAGAAATTGC TTGCAGAGTT TGCATCACGA 
CAGAAAAGCT TCATGGAAAC TGCCATGGAT GTTGATTCTC CAGAGAGTGA TATTCCTATG 
GAGATCACCA CAGCAGAACC TCAAGTTTCT GAGGCAGTAT ATGATTGTGT TATTTGTGGA 
CAGAGTGGGC CCTCCTCTGA AGACAGACCT ACGGGATTGG TTGTCCTGTT ACAAGCATCC 
TCAGTTTTGG GGCAGTGCCG TGACAACATT GAGCCAAAAA AGTTGCCTAT CTCTGAAGAG 
GAGCAGATAT ACCCCTGGGA TACATGTGCA GCAGTTCATG ACGTGAGGCT CTCATTATTA 
CAACGTTATT TTAAGGATAG TTCTTGTCTC TTGGCAGTAT CAATTGGCTG GGAAGGAGGT 
GTTTATGTAC AAACCTGTGG CCACACATTA CATATCGATT GCCATAAATC TTACATGGAA 
TCATTACGGA ATGACCAGGT TCTTCAGGGC TTCTCAGTGG ACAAAGGAGA ATTCACATGT 
CCTCTCTGTA GGCAGTTTGC TAACAGTGTT CTTCCTTGTT ATCCTGGAAG CAATGTTGAA 
AACCACCTTT GGCAGCGTCC TAGTAACAAA AGCATACAAG ATCTCATAAA GGAAGTGGAG 
GAGCTGCAAG GACGTCCGGG AGCTTTCCCA GTAAGCATCA GTTCAGAAAC CAACTTAAGT 
AAAGAAATGG AATCTGTAAT GAAAGATATA AAAAATACCA CTCAGAAGAA ATACAGAGAT 
TATAGCAAGA CTCCAGGCTC ACCAGATAAT GATTTTCTCT TTATGTATTC TGTTGCTAGA 
ACCAACTTGG AACTTGAATT AATTCATCGA GGAGGGAATT TATGTTCAGG TGGTGCAAGT 
ACAGCTGGTA AAAGGTCCTG TTTAAATCAG CTGTTCCATG TATTAGCCTT GCACATGCGA 
CTTTATAGCA TTGATTCTGA GTATAATCCT TGGAGAAAGC TCACACAGTT ACTAGAAGAG 
ATGAGTTCGC AACTGGGAAA TGAGGAACAA CAGCCTGAGG TTCCAATTCT TTATCATGAT 
GTTACATCCC TTTTGCTCAT CCAGATCTTA ATGATGCCTC AACCCTTACA CAAAGACCAC 
TTCACCTGCA TTGTGAAGGT GCTTTTTACC CTACTGTATA CACAAGGTCT TGTGGCACTT 
TCAGTTAAAT GCAATGAGGA AGATAGGTCA GCCTGGAAAC ACACAGGAGC CCTCAAAAAG 
AATACTTGTG ATGCAGAAAA GTCTTATGAA GTGTTATTGA GCTTTGTGAT AAGTGAACTG 
TCTAAAGGAA AGTTATACCA TGAAGAAGGA ACTCAGGAGT GTGCAACGAT TATCCCTGTT 
GCTTGGTCTC CTGAATCCAT GGAAAAATAC TTACAGGACT TCTGCTTACC ATTTCTCAGA 
ATCACCAGCC TTCTTCAGCA CCACCTTTTT GGGGAAGATT TACCTAGCTG CCAGGAAGAA 
GAAGAATTTT CAGTTCTTTC CAGCTGCCTG GGACTTCTGC CAACATTCTA CCAAACAGAA 
CATCCATTCA TCAGTGCCTC CTGTCTGGAT TGGCCAGTTC CGGCATTTGA TATTATATCT 
CAGTGGTGTT TTGAGATAAA TTCATTTACT GAAAGACATG CTGAACAAGG AAAGGCGTTG 
CTTATCCAAG AGTCAAAATG GAAATTGCCA CACCTTCTAC AGTTGCCTGA AAATTATAAC 
ACCATTTTCC AGTACTACCA CAGAAAAACC TGTAGTGTCT GCACCAAGGT TCCTAAAGAC 
CCTGCCGTTT GCCTTGTTTG TGGTACTTTT GTATGCCTGA AAGGACTTTG CTGCAAGCAA 
CAAAGTTACT GTGAATGTGT ATTGCATTCT CAGAACTGTG GTGCTGGAAC AGGGATTTTC 
CTTTTGATCA ATGCATCAGT GATTATCATC ATTCGAGGCC ACCGCTTCTG CCTCTGGGGT 
TCTGTGTATT TGGATGCTCA TGGAGAGGAA GACCGAGATC TTAGGCGAGG CAAGCCTCTC 
TACATTTGTA AAGAAAGATA CAAAGTTCTT GAACAACAGT GGATTTCTCA TACTTTTGAT 
CACATCAATA AAAGATGGGG TCCACATTAC AATGGGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR3 ( XM_010801933.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800235.1 Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X3, mRNA.

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CloneID	OBa53812
Clone ID Related Accession (Same CDS sequence)	XM_015462261.1 , XM_010801933.3 , XM_010801933.2
Accession Version	XM_010801933.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase UBR3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801933.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAATTCT GGTACAGCTC AGGTTTTCAG CTTAGTAGCA 
GAGCGCAGAA AGAAGTTTCA AGAGATCATC AATCGCAGTA ACAGTGAAGC AAATCAAGTG 
GTTCGTCCTA AAACTTCAAG TAAATGGTCT GCACCTGGTT CAGCTCCACA GTTAACTACA 
GCCATTTTGG AAATTAAAGA AAGTATATTG TCTTTGTTAA TTAAACTTCA CCACAAACTC 
TCAGGAAAAC AAAATTCCTA CTATCCTCCT TGGCTTGATG ACATAGAAAT TTTAATCCAA 
CCAGAAATTC CTAAATATAG TCATGGAGAT GGTATAACTG CAGTAGAAAG AATTTTACTA 
AAAGCTGCAT TGCAAAGCAG AATGAACAAA CGCATCATTG AAGAGATATG TAGAAAAGTG 
ACCCCTCCTG TACCACCCAA AAAGATTACT GCAGCAGAGA AGAAAACACT GGACAAAGAA 
GAAAGGCGAC AAAAAGCTAG AGAAAGACAG CAGAAATTGC TTGCAGAGTT TGCATCACGA 
CAGAAAAGCT TCATGGAAAC TGCCATGGAT GTTGATTCTC CAGAGAGTGA TATTCCTATG 
GAGATCACCA CAGCAGAACC TCAAGTTTCT GAGGCAGTAT ATGATTGTGT TATTTGTGGA 
CAGAGTGGGC CCTCCTCTGA AGACAGACCT ACGGGATTGG TTGTCCTGTT ACAAGCATCC 
TCAGTTTTGG GGCAGTGCCG TGACAACATT GAGCCAAAAA AGTTGCCTAT CTCTGAAGAG 
GAGCAGATAT ACCCCTGGGA TACATGTGCA GCAGTTCATG ACGTGAGGCT CTCATTATTA 
CAACGTTATT TTAAGGATAG TTCTTGTCTC TTGGCAGTAT CAATTGGCTG GGAAGGAGGT 
GTTTATGTAC AAACCTGTGG CCACACATTA CATATCGATT GCCATAAATC TTACATGGAA 
TCATTACGGA ATGACCAGGT TCTTCAGGGC TTCTCAGTGG ACAAAGGAGA ATTCACATGT 
CCTCTCTGTA GGCAGTTTGC TAACAGTGTT CTTCCTTGTT ATCCTGGAAG CAATGTTGAA 
AACCACCTTT GGCAGCGTCC TAGTAACAAA AGCATACAAG ATCTCATAAA GGAAGTGGAG 
GAGCTGCAAG GACGTCCGGG AGCTTTCCCA GTAAGCATCA GTTCAGAAAC CAACTTAAGT 
AAAGAAATGG AATCTGTAAT GAAAGATATA AAAAATACCA CTCAGAAGAA ATACAGAGAT 
TATAGCAAGA CTCCAGGCTC ACCAGATAAT GATTTTCTCT TTATGTATTC TGTTGCTAGA 
ACCAACTTGG AACTTGAATT AATTCATCGA GGAGGGAATT TATGTTCAGG TGGTGCAAGT 
ACAGCTGGTA AAAGGTCCTG TTTAAATCAG CTGTTCCATG TATTAGCCTT GCACATGCGA 
CTTTATAGCA TTGATTCTGA GTATAATCCT TGGAGAAAGC TCACACAGTT ACTAGAAGAG 
ATGAGTTCGC AACTGGGAAA TGAGGAACAA CAGCCTGAGG TTCCAATTCT TTATCATGAT 
GTTACATCCC TTTTGCTCAT CCAGATCTTA ATGATGCCTC AACCCTTACA CAAAGACCAC 
TTCACCTGCA TTGTGAAGGT GCTTTTTACC CTACTGTATA CACAAGGTCT TGTGGCACTT 
TCAGTTAAAT GCAATGAGGA AGATAGGTCA GCCTGGAAAC ACACAGGAGC CCTCAAAAAG 
AATACTTGTG ATGCAGAAAA GTCTTATGAA GTGTTATTGA GCTTTGTGAT AAGTGAACTG 
TCTAAAGGAA AGTTATACCA TGAAGAAGGA ACTCAGGAGT GTGCAACGAT TATCCCTGTT 
GCTTGGTCTC CTGAATCCAT GGAAAAATAC TTACAGGACT TCTGCTTACC ATTTCTCAGA 
ATCACCAGCC TTCTTCAGCA CCACCTTTTT GGGGAAGATT TACCTAGCTG CCAGGAAGAA 
GAAGAATTTT CAGTTCTTTC CAGCTGCCTG GGACTTCTGC CAACATTCTA CCAAACAGAA 
CATCCATTCA TCAGTGCCTC CTGTCTGGAT TGGCCAGTTC CGGCATTTGA TATTATATCT 
CAGTGGTGTT TTGAGATAAA TTCATTTACT GAAAGACATG CTGAACAAGG AAAGGCGTTG 
CTTATCCAAG AGTCAAAATG GAAATTGCCA CACCTTCTAC AGTTGCCTGA AAATTATAAC 
ACCATTTTCC AGTACTACCA CAGAAAAACC TGTAGTGTCT GCACCAAGGT TCCTAAAGAC 
CCTGCCGTTT GCCTTGTTTG TGGTACTTTT GTATGCCTGA AAGGACTTTG CTGCAAGCAA 
CAAAGTTACT GTGAATGTGT ATTGCATTCT CAGAACTGTG GTGCTGGAAC AGGGATTTTC 
CTTTTGATCA ATGCATCAGT GATTATCATC ATTCGAGGCC ACCGCTTCTG CCTCTGGGGT 
TCTGTGTATT TGGATGCTCA TGGAGAGGAA GACCGAGATC TTAGGCGAGG CAAGCCTCTC 
TACATTTGTA AAGAAAGATA CAAAGTTCTT GAACAACAGT GGATTTCTCA TACTTTTGAT 
CACATCAATA AAAGATGGGG TCCACATTAC AATGGGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800235.1
CDS	40..5721
Translation	MAAAAAAAVGGPQPPQAEVPAPGLALDKAATAAHLKAALSRPDNRAGAEELQALLERVLSAERPLAAAADGEEAAAAGGGGGPGAAEEEALEWCKCLLAGGGGYDEFCAAVRAYDPAALCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGDHTGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHQIKSSSNIPCVPKDLLMMSEFVLPRFIFCLIQYLREGYNEPAADVPSEKDLNKVLQLLEPQISFLEDLTKMGGAMRSVLTQVLTNQQNYKDLTSGLGENACARKSHEKYLIALKSSGLTYPEDKLVYGIQEPSAGASTLAVQGFASAAGTLGQLDSSDEEDQDGSQGLGKRKRVKLSSNTKDQSIMDVLKHKSFLEELLFWTIKYEFPQKMVTFLLNMLPDQEYKVAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHGLLVTWMNFVSFFQGMNLNKRELNEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVRETQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAVKCQELDLDSVLPDQEMLMKLMIHPLQIQASLAEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNTVLDAEHERSMLEGALTFLVILLSLRLHLGMSDDEILRAEMVAQLCMNDRTHSSLLDLIPENPNPKSGIIPGSYSFESVLSAVADFKAPVFEPGGSMQQGMYTPKAEVWDHEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGKFPGNPWPPYKKRTPLHPSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNLSEHVLCMVLYLIELGLENSAEEESDEEASACGPERCHDSWFPGSNLVSNMRHFINYVRVRVPETAPEVKRDSLASTSSDSLGSLQNSGTAQVFSLVAERRKKFQEIINRSNSEANQVVRPKTSSKWSAPGSAPQLTTAILEIKESILSLLIKLHHKLSGKQNSYYPPWLDDIEILIQPEIPKYSHGDGITAVERILLKAALQSRMNKRIIEEICRKVTPPVPPKKITAAEKKTLDKEERRQKARERQQKLLAEFASRQKSFMETAMDVDSPESDIPMEITTAEPQVSEAVYDCVICGQSGPSSEDRPTGLVVLLQASSVLGQCRDNIEPKKLPISEEEQIYPWDTCAAVHDVRLSLLQRYFKDSSCLLAVSIGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCYPGSNVENHLWQRPSNKSIQDLIKEVEELQGRPGAFPVSISSETNLSKEMESVMKDIKNTTQKKYRDYSKTPGSPDNDFLFMYSVARTNLELELIHRGGNLCSGGASTAGKRSCLNQLFHVLALHMRLYSIDSEYNPWRKLTQLLEEMSSQLGNEEQQPEVPILYHDVTSLLLIQILMMPQPLHKDHFTCIVKVLFTLLYTQGLVALSVKCNEEDRSAWKHTGALKKNTCDAEKSYEVLLSFVISELSKGKLYHEEGTQECATIIPVAWSPESMEKYLQDFCLPFLRITSLLQHHLFGEDLPSCQEEEEFSVLSSCLGLLPTFYQTEHPFISASCLDWPVPAFDIISQWCFEINSFTERHAEQGKALLIQESKWKLPHLLQLPENYNTIFQYYHRKTCSVCTKVPKDPAVCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYKVLEQQWISHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_010801933.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801933.2

ORF Insert Sequence:

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAATTCT GGTACAGCTC AGGTTTTCAG CTTAGTAGCA 
GAGCGCAGAA AGAAGTTTCA AGAGATCATC AATCGCAGTA ACAGTGAAGC AAATCAAGTG 
GTTCGTCCTA AAACTTCAAG TAAATGGTCT GCACCTGGTT CAGCTCCACA GTTAACTACA 
GCCATTTTGG AAATTAAAGA AAGTATATTG TCTTTGTTAA TTAAACTTCA CCACAAACTC 
TCAGGAAAAC AAAATTCCTA CTATCCTCCT TGGCTTGATG ACATAGAAAT TTTAATCCAA 
CCAGAAATTC CTAAATATAG TCATGGAGAT GGTATAACTG CAGTAGAAAG AATTTTACTA 
AAAGCTGCAT TGCAAAGCAG AATGAACAAA CGCATCATTG AAGAGATATG TAGAAAAGTG 
ACCCCTCCTG TACCACCCAA AAAGATTACT GCAGCAGAGA AGAAAACACT GGACAAAGAA 
GAAAGGCGAC AAAAAGCTAG AGAAAGACAG CAGAAATTGC TTGCAGAGTT TGCATCACGA 
CAGAAAAGCT TCATGGAAAC TGCCATGGAT GTTGATTCTC CAGAGAGTGA TATTCCTATG 
GAGATCACCA CAGCAGAACC TCAAGTTTCT GAGGCAGTAT ATGATTGTGT TATTTGTGGA 
CAGAGTGGGC CCTCCTCTGA AGACAGACCT ACGGGATTGG TTGTCCTGTT ACAAGCATCC 
TCAGTTTTGG GGCAGTGCCG TGACAACATT GAGCCAAAAA AGTTGCCTAT CTCTGAAGAG 
GAGCAGATAT ACCCCTGGGA TACATGTGCA GCAGTTCATG ACGTGAGGCT CTCATTATTA 
CAACGTTATT TTAAGGATAG TTCTTGTCTC TTGGCAGTAT CAATTGGCTG GGAAGGAGGT 
GTTTATGTAC AAACCTGTGG CCACACATTA CATATCGATT GCCATAAATC TTACATGGAA 
TCATTACGGA ATGACCAGGT TCTTCAGGGC TTCTCAGTGG ACAAAGGAGA ATTCACATGT 
CCTCTCTGTA GGCAGTTTGC TAACAGTGTT CTTCCTTGTT ATCCTGGAAG CAATGTTGAA 
AACCACCTTT GGCAGCGTCC TAGTAACAAA AGCATACAAG ATCTCATAAA GGAAGTGGAG 
GAGCTGCAAG GACGTCCGGG AGCTTTCCCA GTAAGCATCA GTTCAGAAAC CAACTTAAGT 
AAAGAAATGG AATCTGTAAT GAAAGATATA AAAAATACCA CTCAGAAGAA ATACAGAGAT 
TATAGCAAGA CTCCAGGCTC ACCAGATAAT GATTTTCTCT TTATGTATTC TGTTGCTAGA 
ACCAACTTGG AACTTGAATT AATTCATCGA GGAGGGAATT TATGTTCAGG TGGTGCAAGT 
ACAGCTGGTA AAAGGTCCTG TTTAAATCAG CTGTTCCATG TATTAGCCTT GCACATGCGA 
CTTTATAGCA TTGATTCTGA GTATAATCCT TGGAGAAAGC TCACACAGTT ACTAGAAGAG 
ATGAGTTCGC AACTGGGAAA TGAGGAACAA CAGCCTGAGG TTCCAATTCT TTATCATGAT 
GTTACATCCC TTTTGCTCAT CCAGATCTTA ATGATGCCTC AACCCTTACA CAAAGACCAC 
TTCACCTGCA TTGTGAAGGT GCTTTTTACC CTACTGTATA CACAAGGTCT TGTGGCACTT 
TCAGTTAAAT GCAATGAGGA AGATAGGTCA GCCTGGAAAC ACACAGGAGC CCTCAAAAAG 
AATACTTGTG ATGCAGAAAA GTCTTATGAA GTGTTATTGA GCTTTGTGAT AAGTGAACTG 
TCTAAAGGAA AGTTATACCA TGAAGAAGGA ACTCAGGAGT GTGCAACGAT TATCCCTGTT 
GCTTGGTCTC CTGAATCCAT GGAAAAATAC TTACAGGACT TCTGCTTACC ATTTCTCAGA 
ATCACCAGCC TTCTTCAGCA CCACCTTTTT GGGGAAGATT TACCTAGCTG CCAGGAAGAA 
GAAGAATTTT CAGTTCTTTC CAGCTGCCTG GGACTTCTGC CAACATTCTA CCAAACAGAA 
CATCCATTCA TCAGTGCCTC CTGTCTGGAT TGGCCAGTTC CGGCATTTGA TATTATATCT 
CAGTGGTGTT TTGAGATAAA TTCATTTACT GAAAGACATG CTGAACAAGG AAAGGCGTTG 
CTTATCCAAG AGTCAAAATG GAAATTGCCA CACCTTCTAC AGTTGCCTGA AAATTATAAC 
ACCATTTTCC AGTACTACCA CAGAAAAACC TGTAGTGTCT GCACCAAGGT TCCTAAAGAC 
CCTGCCGTTT GCCTTGTTTG TGGTACTTTT GTATGCCTGA AAGGACTTTG CTGCAAGCAA 
CAAAGTTACT GTGAATGTGT ATTGCATTCT CAGAACTGTG GTGCTGGAAC AGGGATTTTC 
CTTTTGATCA ATGCATCAGT GATTATCATC ATTCGAGGCC ACCGCTTCTG CCTCTGGGGT 
TCTGTGTATT TGGATGCTCA TGGAGAGGAA GACCGAGATC TTAGGCGAGG CAAGCCTCTC 
TACATTTGTA AAGAAAGATA CAAAGTTCTT GAACAACAGT GGATTTCTCA TACTTTTGAT 
CACATCAATA AAAGATGGGG TCCACATTAC AATGGGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR3 ( XM_010826431.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010824733.2 Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X4, mRNA.

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CloneID	OBa53813
Clone ID Related Accession (Same CDS sequence)	XM_010826431.2 , XM_010801934.2 , XM_010801934.3
Accession Version	XM_010826431.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase UBR3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644584.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826431.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAATTCT GGTACAGCTC AGGTTTTCAG CTTAGTAGCA 
GAGCGCAGAA AGAAGTTTCA AGAGATCATC AATCGCAGTA ACAGTGAAGC AAATCAAGTG 
GTTCGTCCTA AAACTTCAAG TAAATGGTCT GCACCTGGTT CAGCTCCACA GTTAACTACA 
GCCATTTTGG AAATTAAAGA AAGTATATTG TCTTTGTTAA TTAAACTTCA CCACAAACTC 
TCAGGAAAAC AAAATTCCTA CTATCCTCCT TGGCTTGATG ACATAGAAAT TTTAATCCAA 
CCAGAAATTC CTAAATATAG TCATGGAGAT GGTATAACTG CAGTAGAAAG AATTTTACTA 
AAAGCTGCAT TGCAAAGCAG AATGAACAAA CGCATCATTG AAGAGATATG TAGAAAAGTG 
ACCCCTCCTG TACCACCCAA AAAGATTACT GCAGCAGAGA AGAAAACACT GGACAAAGAA 
GAAAGGCGAC AAAAAGCTAG AGAAAGACAG CAGAAATTGC TTGCAGAGTT TGCATCACGA 
CAGAAAAGCT TCATGGAAAC TGCCATGGAT GTTGATTCTC CAGAGAGTGA TATTCCTATG 
GAGATCACCA CAGCAGAACC TCAAGTTTCT GAGGCAGTAT ATGATTGTGT TATTTGTGGA 
CAGAGTGGGC CCTCCTCTGA AGACAGACCT ACGGGATTGG TTGTCCTGTT ACAAGCATCC 
TCAGTTTTGG GGCAGTGCCG TGACAACATT GAGCCAAAAA AGTTGCCTAT CTCTGAAGAG 
GAGCAGATAT ACCCCTGGGA TACATGTGCA GCAGTTCATG ACGTGAGGCT CTCATTATTA 
CAACGTTATT TTAAGGATAG TTCTTGTCTC TTGGCAGTAT CAATTGGCTG GGAAGGAGGT 
GTTTATGTAC AAACCTGTGG CCACACATTA CATATCGATT GCCATAAATC TTACATGGAA 
TCATTACGGA ATGACCAGGT TCTTCAGGGC TTCTCAGTGG ACAAAGGAGA ATTCACATGT 
CCTCTCTGTA GGCAGTTTGC TAACAGTGTT CTTCCTTGTT ATCCTGGAAG CAATGTTGAA 
AACCACCTTT GGCAGCGTCC TAGTAACAAA AGCATACAAG ATCTCATAAA GGAAGTGGAG 
GAGCTGCAAG GACGTCCGGG AGCTTTCCCA TCAGAAACCA ACTTAAGTAA AGAAATGGAA 
TCTGTAATGA AAGATATAAA AAATACCACT CAGAAGAAAT ACAGAGATTA TAGCAAGACT 
CCAGGCTCAC CAGATAATGA TTTTCTCTTT ATGTATTCTG TTGCTAGAAC CAACTTGGAA 
CTTGAATTAA TTCATCGAGG AGGGAATTTA TGTTCAGGTG GTGCAAGTAC AGCTGGTAAA 
AGGTCCTGTT TAAATCAGCT GTTCCATGTA TTAGCCTTGC ACATGCGACT TTATAGCATT 
GATTCTGAGT ATAATCCTTG GAGAAAGCTC ACACAGTTAC TAGAAGAGAT GAGTTCGCAA 
CTGGGAAATG AGGAACAACA GCCTGAGGTT CCAATTCTTT ATCATGATGT TACATCCCTT 
TTGCTCATCC AGATCTTAAT GATGCCTCAA CCCTTACACA AAGACCACTT CACCTGCATT 
GTGAAGGTGC TTTTTACCCT ACTGTATACA CAAGGTCTTG TGGCACTTTC AGTTAAATGC 
AATGAGGAAG ATAGGTCAGC CTGGAAACAC ACAGGAGCCC TCAAAAAGAA TACTTGTGAT 
GCAGAAAAGT CTTATGAAGT GTTATTGAGC TTTGTGATAA GTGAACTGTC TAAAGGAAAG 
TTATACCATG AAGAAGGAAC TCAGGAGTGT GCAACGATTA TCCCTGTTGC TTGGTCTCCT 
GAATCCATGG AAAAATACTT ACAGGACTTC TGCTTACCAT TTCTCAGAAT CACCAGCCTT 
CTTCAGCACC ACCTTTTTGG GGAAGATTTA CCTAGCTGCC AGGAAGAAGA AGAATTTTCA 
GTTCTTTCCA GCTGCCTGGG ACTTCTGCCA ACATTCTACC AAACAGAACA TCCATTCATC 
AGTGCCTCCT GTCTGGATTG GCCAGTTCCG GCATTTGATA TTATATCTCA GTGGTGTTTT 
GAGATAAATT CATTTACTGA AAGACATGCT GAACAAGGAA AGGCGTTGCT TATCCAAGAG 
TCAAAATGGA AATTGCCACA CCTTCTACAG TTGCCTGAAA ATTATAACAC CATTTTCCAG 
TACTACCACA GAAAAACCTG TAGTGTCTGC ACCAAGGTTC CTAAAGACCC TGCCGTTTGC 
CTTGTTTGTG GTACTTTTGT ATGCCTGAAA GGACTTTGCT GCAAGCAACA AAGTTACTGT 
GAATGTGTAT TGCATTCTCA GAACTGTGGT GCTGGAACAG GGATTTTCCT TTTGATCAAT 
GCATCAGTGA TTATCATCAT TCGAGGCCAC CGCTTCTGCC TCTGGGGTTC TGTGTATTTG 
GATGCTCATG GAGAGGAAGA CCGAGATCTT AGGCGAGGCA AGCCTCTCTA CATTTGTAAA 
GAAAGATACA AAGTTCTTGA ACAACAGTGG ATTTCTCATA CTTTTGATCA CATCAATAAA 
AGATGGGGTC CACATTACAA TGGGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010824733.2
CDS	40..5709
Translation	MAAAAAAAVGGPQPPQAEVPAPGLALDKAATAAHLKAALSRPDNRAGAEELQALLERVLSAERPLAAAADGEEAAAAGGGGGPGAAEEEALEWCKCLLAGGGGYDEFCAAVRAYDPAALCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGDHTGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHQIKSSSNIPCVPKDLLMMSEFVLPRFIFCLIQYLREGYNEPAADVPSEKDLNKVLQLLEPQISFLEDLTKMGGAMRSVLTQVLTNQQNYKDLTSGLGENACARKSHEKYLIALKSSGLTYPEDKLVYGIQEPSAGASTLAVQGFASAAGTLGQLDSSDEEDQDGSQGLGKRKRVKLSSNTKDQSIMDVLKHKSFLEELLFWTIKYEFPQKMVTFLLNMLPDQEYKVAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHGLLVTWMNFVSFFQGMNLNKRELNEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVRETQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAVKCQELDLDSVLPDQEMLMKLMIHPLQIQASLAEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNTVLDAEHERSMLEGALTFLVILLSLRLHLGMSDDEILRAEMVAQLCMNDRTHSSLLDLIPENPNPKSGIIPGSYSFESVLSAVADFKAPVFEPGGSMQQGMYTPKAEVWDHEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGKFPGNPWPPYKKRTPLHPSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNLSEHVLCMVLYLIELGLENSAEEESDEEASACGPERCHDSWFPGSNLVSNMRHFINYVRVRVPETAPEVKRDSLASTSSDSLGSLQNSGTAQVFSLVAERRKKFQEIINRSNSEANQVVRPKTSSKWSAPGSAPQLTTAILEIKESILSLLIKLHHKLSGKQNSYYPPWLDDIEILIQPEIPKYSHGDGITAVERILLKAALQSRMNKRIIEEICRKVTPPVPPKKITAAEKKTLDKEERRQKARERQQKLLAEFASRQKSFMETAMDVDSPESDIPMEITTAEPQVSEAVYDCVICGQSGPSSEDRPTGLVVLLQASSVLGQCRDNIEPKKLPISEEEQIYPWDTCAAVHDVRLSLLQRYFKDSSCLLAVSIGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCYPGSNVENHLWQRPSNKSIQDLIKEVEELQGRPGAFPSETNLSKEMESVMKDIKNTTQKKYRDYSKTPGSPDNDFLFMYSVARTNLELELIHRGGNLCSGGASTAGKRSCLNQLFHVLALHMRLYSIDSEYNPWRKLTQLLEEMSSQLGNEEQQPEVPILYHDVTSLLLIQILMMPQPLHKDHFTCIVKVLFTLLYTQGLVALSVKCNEEDRSAWKHTGALKKNTCDAEKSYEVLLSFVISELSKGKLYHEEGTQECATIIPVAWSPESMEKYLQDFCLPFLRITSLLQHHLFGEDLPSCQEEEEFSVLSSCLGLLPTFYQTEHPFISASCLDWPVPAFDIISQWCFEINSFTERHAEQGKALLIQESKWKLPHLLQLPENYNTIFQYYHRKTCSVCTKVPKDPAVCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYKVLEQQWISHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_010826431.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010826431.2

ORF Insert Sequence:

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAATTCT GGTACAGCTC AGGTTTTCAG CTTAGTAGCA 
GAGCGCAGAA AGAAGTTTCA AGAGATCATC AATCGCAGTA ACAGTGAAGC AAATCAAGTG 
GTTCGTCCTA AAACTTCAAG TAAATGGTCT GCACCTGGTT CAGCTCCACA GTTAACTACA 
GCCATTTTGG AAATTAAAGA AAGTATATTG TCTTTGTTAA TTAAACTTCA CCACAAACTC 
TCAGGAAAAC AAAATTCCTA CTATCCTCCT TGGCTTGATG ACATAGAAAT TTTAATCCAA 
CCAGAAATTC CTAAATATAG TCATGGAGAT GGTATAACTG CAGTAGAAAG AATTTTACTA 
AAAGCTGCAT TGCAAAGCAG AATGAACAAA CGCATCATTG AAGAGATATG TAGAAAAGTG 
ACCCCTCCTG TACCACCCAA AAAGATTACT GCAGCAGAGA AGAAAACACT GGACAAAGAA 
GAAAGGCGAC AAAAAGCTAG AGAAAGACAG CAGAAATTGC TTGCAGAGTT TGCATCACGA 
CAGAAAAGCT TCATGGAAAC TGCCATGGAT GTTGATTCTC CAGAGAGTGA TATTCCTATG 
GAGATCACCA CAGCAGAACC TCAAGTTTCT GAGGCAGTAT ATGATTGTGT TATTTGTGGA 
CAGAGTGGGC CCTCCTCTGA AGACAGACCT ACGGGATTGG TTGTCCTGTT ACAAGCATCC 
TCAGTTTTGG GGCAGTGCCG TGACAACATT GAGCCAAAAA AGTTGCCTAT CTCTGAAGAG 
GAGCAGATAT ACCCCTGGGA TACATGTGCA GCAGTTCATG ACGTGAGGCT CTCATTATTA 
CAACGTTATT TTAAGGATAG TTCTTGTCTC TTGGCAGTAT CAATTGGCTG GGAAGGAGGT 
GTTTATGTAC AAACCTGTGG CCACACATTA CATATCGATT GCCATAAATC TTACATGGAA 
TCATTACGGA ATGACCAGGT TCTTCAGGGC TTCTCAGTGG ACAAAGGAGA ATTCACATGT 
CCTCTCTGTA GGCAGTTTGC TAACAGTGTT CTTCCTTGTT ATCCTGGAAG CAATGTTGAA 
AACCACCTTT GGCAGCGTCC TAGTAACAAA AGCATACAAG ATCTCATAAA GGAAGTGGAG 
GAGCTGCAAG GACGTCCGGG AGCTTTCCCA TCAGAAACCA ACTTAAGTAA AGAAATGGAA 
TCTGTAATGA AAGATATAAA AAATACCACT CAGAAGAAAT ACAGAGATTA TAGCAAGACT 
CCAGGCTCAC CAGATAATGA TTTTCTCTTT ATGTATTCTG TTGCTAGAAC CAACTTGGAA 
CTTGAATTAA TTCATCGAGG AGGGAATTTA TGTTCAGGTG GTGCAAGTAC AGCTGGTAAA 
AGGTCCTGTT TAAATCAGCT GTTCCATGTA TTAGCCTTGC ACATGCGACT TTATAGCATT 
GATTCTGAGT ATAATCCTTG GAGAAAGCTC ACACAGTTAC TAGAAGAGAT GAGTTCGCAA 
CTGGGAAATG AGGAACAACA GCCTGAGGTT CCAATTCTTT ATCATGATGT TACATCCCTT 
TTGCTCATCC AGATCTTAAT GATGCCTCAA CCCTTACACA AAGACCACTT CACCTGCATT 
GTGAAGGTGC TTTTTACCCT ACTGTATACA CAAGGTCTTG TGGCACTTTC AGTTAAATGC 
AATGAGGAAG ATAGGTCAGC CTGGAAACAC ACAGGAGCCC TCAAAAAGAA TACTTGTGAT 
GCAGAAAAGT CTTATGAAGT GTTATTGAGC TTTGTGATAA GTGAACTGTC TAAAGGAAAG 
TTATACCATG AAGAAGGAAC TCAGGAGTGT GCAACGATTA TCCCTGTTGC TTGGTCTCCT 
GAATCCATGG AAAAATACTT ACAGGACTTC TGCTTACCAT TTCTCAGAAT CACCAGCCTT 
CTTCAGCACC ACCTTTTTGG GGAAGATTTA CCTAGCTGCC AGGAAGAAGA AGAATTTTCA 
GTTCTTTCCA GCTGCCTGGG ACTTCTGCCA ACATTCTACC AAACAGAACA TCCATTCATC 
AGTGCCTCCT GTCTGGATTG GCCAGTTCCG GCATTTGATA TTATATCTCA GTGGTGTTTT 
GAGATAAATT CATTTACTGA AAGACATGCT GAACAAGGAA AGGCGTTGCT TATCCAAGAG 
TCAAAATGGA AATTGCCACA CCTTCTACAG TTGCCTGAAA ATTATAACAC CATTTTCCAG 
TACTACCACA GAAAAACCTG TAGTGTCTGC ACCAAGGTTC CTAAAGACCC TGCCGTTTGC 
CTTGTTTGTG GTACTTTTGT ATGCCTGAAA GGACTTTGCT GCAAGCAACA AAGTTACTGT 
GAATGTGTAT TGCATTCTCA GAACTGTGGT GCTGGAACAG GGATTTTCCT TTTGATCAAT 
GCATCAGTGA TTATCATCAT TCGAGGCCAC CGCTTCTGCC TCTGGGGTTC TGTGTATTTG 
GATGCTCATG GAGAGGAAGA CCGAGATCTT AGGCGAGGCA AGCCTCTCTA CATTTGTAAA 
GAAAGATACA AAGTTCTTGA ACAACAGTGG ATTTCTCATA CTTTTGATCA CATCAATAAA 
AGATGGGGTC CACATTACAA TGGGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR3 ( XM_010801934.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800236.1 Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X4, mRNA.

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CloneID	OBa53813
Clone ID Related Accession (Same CDS sequence)	XM_010826431.2 , XM_010801934.2 , XM_010801934.3
Accession Version	XM_010801934.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase UBR3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103836.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801934.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAATTCT GGTACAGCTC AGGTTTTCAG CTTAGTAGCA 
GAGCGCAGAA AGAAGTTTCA AGAGATCATC AATCGCAGTA ACAGTGAAGC AAATCAAGTG 
GTTCGTCCTA AAACTTCAAG TAAATGGTCT GCACCTGGTT CAGCTCCACA GTTAACTACA 
GCCATTTTGG AAATTAAAGA AAGTATATTG TCTTTGTTAA TTAAACTTCA CCACAAACTC 
TCAGGAAAAC AAAATTCCTA CTATCCTCCT TGGCTTGATG ACATAGAAAT TTTAATCCAA 
CCAGAAATTC CTAAATATAG TCATGGAGAT GGTATAACTG CAGTAGAAAG AATTTTACTA 
AAAGCTGCAT TGCAAAGCAG AATGAACAAA CGCATCATTG AAGAGATATG TAGAAAAGTG 
ACCCCTCCTG TACCACCCAA AAAGATTACT GCAGCAGAGA AGAAAACACT GGACAAAGAA 
GAAAGGCGAC AAAAAGCTAG AGAAAGACAG CAGAAATTGC TTGCAGAGTT TGCATCACGA 
CAGAAAAGCT TCATGGAAAC TGCCATGGAT GTTGATTCTC CAGAGAGTGA TATTCCTATG 
GAGATCACCA CAGCAGAACC TCAAGTTTCT GAGGCAGTAT ATGATTGTGT TATTTGTGGA 
CAGAGTGGGC CCTCCTCTGA AGACAGACCT ACGGGATTGG TTGTCCTGTT ACAAGCATCC 
TCAGTTTTGG GGCAGTGCCG TGACAACATT GAGCCAAAAA AGTTGCCTAT CTCTGAAGAG 
GAGCAGATAT ACCCCTGGGA TACATGTGCA GCAGTTCATG ACGTGAGGCT CTCATTATTA 
CAACGTTATT TTAAGGATAG TTCTTGTCTC TTGGCAGTAT CAATTGGCTG GGAAGGAGGT 
GTTTATGTAC AAACCTGTGG CCACACATTA CATATCGATT GCCATAAATC TTACATGGAA 
TCATTACGGA ATGACCAGGT TCTTCAGGGC TTCTCAGTGG ACAAAGGAGA ATTCACATGT 
CCTCTCTGTA GGCAGTTTGC TAACAGTGTT CTTCCTTGTT ATCCTGGAAG CAATGTTGAA 
AACCACCTTT GGCAGCGTCC TAGTAACAAA AGCATACAAG ATCTCATAAA GGAAGTGGAG 
GAGCTGCAAG GACGTCCGGG AGCTTTCCCA TCAGAAACCA ACTTAAGTAA AGAAATGGAA 
TCTGTAATGA AAGATATAAA AAATACCACT CAGAAGAAAT ACAGAGATTA TAGCAAGACT 
CCAGGCTCAC CAGATAATGA TTTTCTCTTT ATGTATTCTG TTGCTAGAAC CAACTTGGAA 
CTTGAATTAA TTCATCGAGG AGGGAATTTA TGTTCAGGTG GTGCAAGTAC AGCTGGTAAA 
AGGTCCTGTT TAAATCAGCT GTTCCATGTA TTAGCCTTGC ACATGCGACT TTATAGCATT 
GATTCTGAGT ATAATCCTTG GAGAAAGCTC ACACAGTTAC TAGAAGAGAT GAGTTCGCAA 
CTGGGAAATG AGGAACAACA GCCTGAGGTT CCAATTCTTT ATCATGATGT TACATCCCTT 
TTGCTCATCC AGATCTTAAT GATGCCTCAA CCCTTACACA AAGACCACTT CACCTGCATT 
GTGAAGGTGC TTTTTACCCT ACTGTATACA CAAGGTCTTG TGGCACTTTC AGTTAAATGC 
AATGAGGAAG ATAGGTCAGC CTGGAAACAC ACAGGAGCCC TCAAAAAGAA TACTTGTGAT 
GCAGAAAAGT CTTATGAAGT GTTATTGAGC TTTGTGATAA GTGAACTGTC TAAAGGAAAG 
TTATACCATG AAGAAGGAAC TCAGGAGTGT GCAACGATTA TCCCTGTTGC TTGGTCTCCT 
GAATCCATGG AAAAATACTT ACAGGACTTC TGCTTACCAT TTCTCAGAAT CACCAGCCTT 
CTTCAGCACC ACCTTTTTGG GGAAGATTTA CCTAGCTGCC AGGAAGAAGA AGAATTTTCA 
GTTCTTTCCA GCTGCCTGGG ACTTCTGCCA ACATTCTACC AAACAGAACA TCCATTCATC 
AGTGCCTCCT GTCTGGATTG GCCAGTTCCG GCATTTGATA TTATATCTCA GTGGTGTTTT 
GAGATAAATT CATTTACTGA AAGACATGCT GAACAAGGAA AGGCGTTGCT TATCCAAGAG 
TCAAAATGGA AATTGCCACA CCTTCTACAG TTGCCTGAAA ATTATAACAC CATTTTCCAG 
TACTACCACA GAAAAACCTG TAGTGTCTGC ACCAAGGTTC CTAAAGACCC TGCCGTTTGC 
CTTGTTTGTG GTACTTTTGT ATGCCTGAAA GGACTTTGCT GCAAGCAACA AAGTTACTGT 
GAATGTGTAT TGCATTCTCA GAACTGTGGT GCTGGAACAG GGATTTTCCT TTTGATCAAT 
GCATCAGTGA TTATCATCAT TCGAGGCCAC CGCTTCTGCC TCTGGGGTTC TGTGTATTTG 
GATGCTCATG GAGAGGAAGA CCGAGATCTT AGGCGAGGCA AGCCTCTCTA CATTTGTAAA 
GAAAGATACA AAGTTCTTGA ACAACAGTGG ATTTCTCATA CTTTTGATCA CATCAATAAA 
AGATGGGGTC CACATTACAA TGGGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800236.1
CDS	40..5709
Translation	MAAAAAAAVGGPQPPQAEVPAPGLALDKAATAAHLKAALSRPDNRAGAEELQALLERVLSAERPLAAAADGEEAAAAGGGGGPGAAEEEALEWCKCLLAGGGGYDEFCAAVRAYDPAALCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGDHTGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHQIKSSSNIPCVPKDLLMMSEFVLPRFIFCLIQYLREGYNEPAADVPSEKDLNKVLQLLEPQISFLEDLTKMGGAMRSVLTQVLTNQQNYKDLTSGLGENACARKSHEKYLIALKSSGLTYPEDKLVYGIQEPSAGASTLAVQGFASAAGTLGQLDSSDEEDQDGSQGLGKRKRVKLSSNTKDQSIMDVLKHKSFLEELLFWTIKYEFPQKMVTFLLNMLPDQEYKVAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHGLLVTWMNFVSFFQGMNLNKRELNEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVRETQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAVKCQELDLDSVLPDQEMLMKLMIHPLQIQASLAEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNTVLDAEHERSMLEGALTFLVILLSLRLHLGMSDDEILRAEMVAQLCMNDRTHSSLLDLIPENPNPKSGIIPGSYSFESVLSAVADFKAPVFEPGGSMQQGMYTPKAEVWDHEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGKFPGNPWPPYKKRTPLHPSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNLSEHVLCMVLYLIELGLENSAEEESDEEASACGPERCHDSWFPGSNLVSNMRHFINYVRVRVPETAPEVKRDSLASTSSDSLGSLQNSGTAQVFSLVAERRKKFQEIINRSNSEANQVVRPKTSSKWSAPGSAPQLTTAILEIKESILSLLIKLHHKLSGKQNSYYPPWLDDIEILIQPEIPKYSHGDGITAVERILLKAALQSRMNKRIIEEICRKVTPPVPPKKITAAEKKTLDKEERRQKARERQQKLLAEFASRQKSFMETAMDVDSPESDIPMEITTAEPQVSEAVYDCVICGQSGPSSEDRPTGLVVLLQASSVLGQCRDNIEPKKLPISEEEQIYPWDTCAAVHDVRLSLLQRYFKDSSCLLAVSIGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCYPGSNVENHLWQRPSNKSIQDLIKEVEELQGRPGAFPSETNLSKEMESVMKDIKNTTQKKYRDYSKTPGSPDNDFLFMYSVARTNLELELIHRGGNLCSGGASTAGKRSCLNQLFHVLALHMRLYSIDSEYNPWRKLTQLLEEMSSQLGNEEQQPEVPILYHDVTSLLLIQILMMPQPLHKDHFTCIVKVLFTLLYTQGLVALSVKCNEEDRSAWKHTGALKKNTCDAEKSYEVLLSFVISELSKGKLYHEEGTQECATIIPVAWSPESMEKYLQDFCLPFLRITSLLQHHLFGEDLPSCQEEEEFSVLSSCLGLLPTFYQTEHPFISASCLDWPVPAFDIISQWCFEINSFTERHAEQGKALLIQESKWKLPHLLQLPENYNTIFQYYHRKTCSVCTKVPKDPAVCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYKVLEQQWISHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_010801934.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801934.2

ORF Insert Sequence:

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAATTCT GGTACAGCTC AGGTTTTCAG CTTAGTAGCA 
GAGCGCAGAA AGAAGTTTCA AGAGATCATC AATCGCAGTA ACAGTGAAGC AAATCAAGTG 
GTTCGTCCTA AAACTTCAAG TAAATGGTCT GCACCTGGTT CAGCTCCACA GTTAACTACA 
GCCATTTTGG AAATTAAAGA AAGTATATTG TCTTTGTTAA TTAAACTTCA CCACAAACTC 
TCAGGAAAAC AAAATTCCTA CTATCCTCCT TGGCTTGATG ACATAGAAAT TTTAATCCAA 
CCAGAAATTC CTAAATATAG TCATGGAGAT GGTATAACTG CAGTAGAAAG AATTTTACTA 
AAAGCTGCAT TGCAAAGCAG AATGAACAAA CGCATCATTG AAGAGATATG TAGAAAAGTG 
ACCCCTCCTG TACCACCCAA AAAGATTACT GCAGCAGAGA AGAAAACACT GGACAAAGAA 
GAAAGGCGAC AAAAAGCTAG AGAAAGACAG CAGAAATTGC TTGCAGAGTT TGCATCACGA 
CAGAAAAGCT TCATGGAAAC TGCCATGGAT GTTGATTCTC CAGAGAGTGA TATTCCTATG 
GAGATCACCA CAGCAGAACC TCAAGTTTCT GAGGCAGTAT ATGATTGTGT TATTTGTGGA 
CAGAGTGGGC CCTCCTCTGA AGACAGACCT ACGGGATTGG TTGTCCTGTT ACAAGCATCC 
TCAGTTTTGG GGCAGTGCCG TGACAACATT GAGCCAAAAA AGTTGCCTAT CTCTGAAGAG 
GAGCAGATAT ACCCCTGGGA TACATGTGCA GCAGTTCATG ACGTGAGGCT CTCATTATTA 
CAACGTTATT TTAAGGATAG TTCTTGTCTC TTGGCAGTAT CAATTGGCTG GGAAGGAGGT 
GTTTATGTAC AAACCTGTGG CCACACATTA CATATCGATT GCCATAAATC TTACATGGAA 
TCATTACGGA ATGACCAGGT TCTTCAGGGC TTCTCAGTGG ACAAAGGAGA ATTCACATGT 
CCTCTCTGTA GGCAGTTTGC TAACAGTGTT CTTCCTTGTT ATCCTGGAAG CAATGTTGAA 
AACCACCTTT GGCAGCGTCC TAGTAACAAA AGCATACAAG ATCTCATAAA GGAAGTGGAG 
GAGCTGCAAG GACGTCCGGG AGCTTTCCCA TCAGAAACCA ACTTAAGTAA AGAAATGGAA 
TCTGTAATGA AAGATATAAA AAATACCACT CAGAAGAAAT ACAGAGATTA TAGCAAGACT 
CCAGGCTCAC CAGATAATGA TTTTCTCTTT ATGTATTCTG TTGCTAGAAC CAACTTGGAA 
CTTGAATTAA TTCATCGAGG AGGGAATTTA TGTTCAGGTG GTGCAAGTAC AGCTGGTAAA 
AGGTCCTGTT TAAATCAGCT GTTCCATGTA TTAGCCTTGC ACATGCGACT TTATAGCATT 
GATTCTGAGT ATAATCCTTG GAGAAAGCTC ACACAGTTAC TAGAAGAGAT GAGTTCGCAA 
CTGGGAAATG AGGAACAACA GCCTGAGGTT CCAATTCTTT ATCATGATGT TACATCCCTT 
TTGCTCATCC AGATCTTAAT GATGCCTCAA CCCTTACACA AAGACCACTT CACCTGCATT 
GTGAAGGTGC TTTTTACCCT ACTGTATACA CAAGGTCTTG TGGCACTTTC AGTTAAATGC 
AATGAGGAAG ATAGGTCAGC CTGGAAACAC ACAGGAGCCC TCAAAAAGAA TACTTGTGAT 
GCAGAAAAGT CTTATGAAGT GTTATTGAGC TTTGTGATAA GTGAACTGTC TAAAGGAAAG 
TTATACCATG AAGAAGGAAC TCAGGAGTGT GCAACGATTA TCCCTGTTGC TTGGTCTCCT 
GAATCCATGG AAAAATACTT ACAGGACTTC TGCTTACCAT TTCTCAGAAT CACCAGCCTT 
CTTCAGCACC ACCTTTTTGG GGAAGATTTA CCTAGCTGCC AGGAAGAAGA AGAATTTTCA 
GTTCTTTCCA GCTGCCTGGG ACTTCTGCCA ACATTCTACC AAACAGAACA TCCATTCATC 
AGTGCCTCCT GTCTGGATTG GCCAGTTCCG GCATTTGATA TTATATCTCA GTGGTGTTTT 
GAGATAAATT CATTTACTGA AAGACATGCT GAACAAGGAA AGGCGTTGCT TATCCAAGAG 
TCAAAATGGA AATTGCCACA CCTTCTACAG TTGCCTGAAA ATTATAACAC CATTTTCCAG 
TACTACCACA GAAAAACCTG TAGTGTCTGC ACCAAGGTTC CTAAAGACCC TGCCGTTTGC 
CTTGTTTGTG GTACTTTTGT ATGCCTGAAA GGACTTTGCT GCAAGCAACA AAGTTACTGT 
GAATGTGTAT TGCATTCTCA GAACTGTGGT GCTGGAACAG GGATTTTCCT TTTGATCAAT 
GCATCAGTGA TTATCATCAT TCGAGGCCAC CGCTTCTGCC TCTGGGGTTC TGTGTATTTG 
GATGCTCATG GAGAGGAAGA CCGAGATCTT AGGCGAGGCA AGCCTCTCTA CATTTGTAAA 
GAAAGATACA AAGTTCTTGA ACAACAGTGG ATTTCTCATA CTTTTGATCA CATCAATAAA 
AGATGGGGTC CACATTACAA TGGGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR3 ( XM_010801934.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800236.1 Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X5, mRNA.

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CloneID	OBa53813
Clone ID Related Accession (Same CDS sequence)	XM_010826431.2 , XM_010801934.2 , XM_010801934.3
Accession Version	XM_010801934.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase UBR3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801934.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAATTCT GGTACAGCTC AGGTTTTCAG CTTAGTAGCA 
GAGCGCAGAA AGAAGTTTCA AGAGATCATC AATCGCAGTA ACAGTGAAGC AAATCAAGTG 
GTTCGTCCTA AAACTTCAAG TAAATGGTCT GCACCTGGTT CAGCTCCACA GTTAACTACA 
GCCATTTTGG AAATTAAAGA AAGTATATTG TCTTTGTTAA TTAAACTTCA CCACAAACTC 
TCAGGAAAAC AAAATTCCTA CTATCCTCCT TGGCTTGATG ACATAGAAAT TTTAATCCAA 
CCAGAAATTC CTAAATATAG TCATGGAGAT GGTATAACTG CAGTAGAAAG AATTTTACTA 
AAAGCTGCAT TGCAAAGCAG AATGAACAAA CGCATCATTG AAGAGATATG TAGAAAAGTG 
ACCCCTCCTG TACCACCCAA AAAGATTACT GCAGCAGAGA AGAAAACACT GGACAAAGAA 
GAAAGGCGAC AAAAAGCTAG AGAAAGACAG CAGAAATTGC TTGCAGAGTT TGCATCACGA 
CAGAAAAGCT TCATGGAAAC TGCCATGGAT GTTGATTCTC CAGAGAGTGA TATTCCTATG 
GAGATCACCA CAGCAGAACC TCAAGTTTCT GAGGCAGTAT ATGATTGTGT TATTTGTGGA 
CAGAGTGGGC CCTCCTCTGA AGACAGACCT ACGGGATTGG TTGTCCTGTT ACAAGCATCC 
TCAGTTTTGG GGCAGTGCCG TGACAACATT GAGCCAAAAA AGTTGCCTAT CTCTGAAGAG 
GAGCAGATAT ACCCCTGGGA TACATGTGCA GCAGTTCATG ACGTGAGGCT CTCATTATTA 
CAACGTTATT TTAAGGATAG TTCTTGTCTC TTGGCAGTAT CAATTGGCTG GGAAGGAGGT 
GTTTATGTAC AAACCTGTGG CCACACATTA CATATCGATT GCCATAAATC TTACATGGAA 
TCATTACGGA ATGACCAGGT TCTTCAGGGC TTCTCAGTGG ACAAAGGAGA ATTCACATGT 
CCTCTCTGTA GGCAGTTTGC TAACAGTGTT CTTCCTTGTT ATCCTGGAAG CAATGTTGAA 
AACCACCTTT GGCAGCGTCC TAGTAACAAA AGCATACAAG ATCTCATAAA GGAAGTGGAG 
GAGCTGCAAG GACGTCCGGG AGCTTTCCCA TCAGAAACCA ACTTAAGTAA AGAAATGGAA 
TCTGTAATGA AAGATATAAA AAATACCACT CAGAAGAAAT ACAGAGATTA TAGCAAGACT 
CCAGGCTCAC CAGATAATGA TTTTCTCTTT ATGTATTCTG TTGCTAGAAC CAACTTGGAA 
CTTGAATTAA TTCATCGAGG AGGGAATTTA TGTTCAGGTG GTGCAAGTAC AGCTGGTAAA 
AGGTCCTGTT TAAATCAGCT GTTCCATGTA TTAGCCTTGC ACATGCGACT TTATAGCATT 
GATTCTGAGT ATAATCCTTG GAGAAAGCTC ACACAGTTAC TAGAAGAGAT GAGTTCGCAA 
CTGGGAAATG AGGAACAACA GCCTGAGGTT CCAATTCTTT ATCATGATGT TACATCCCTT 
TTGCTCATCC AGATCTTAAT GATGCCTCAA CCCTTACACA AAGACCACTT CACCTGCATT 
GTGAAGGTGC TTTTTACCCT ACTGTATACA CAAGGTCTTG TGGCACTTTC AGTTAAATGC 
AATGAGGAAG ATAGGTCAGC CTGGAAACAC ACAGGAGCCC TCAAAAAGAA TACTTGTGAT 
GCAGAAAAGT CTTATGAAGT GTTATTGAGC TTTGTGATAA GTGAACTGTC TAAAGGAAAG 
TTATACCATG AAGAAGGAAC TCAGGAGTGT GCAACGATTA TCCCTGTTGC TTGGTCTCCT 
GAATCCATGG AAAAATACTT ACAGGACTTC TGCTTACCAT TTCTCAGAAT CACCAGCCTT 
CTTCAGCACC ACCTTTTTGG GGAAGATTTA CCTAGCTGCC AGGAAGAAGA AGAATTTTCA 
GTTCTTTCCA GCTGCCTGGG ACTTCTGCCA ACATTCTACC AAACAGAACA TCCATTCATC 
AGTGCCTCCT GTCTGGATTG GCCAGTTCCG GCATTTGATA TTATATCTCA GTGGTGTTTT 
GAGATAAATT CATTTACTGA AAGACATGCT GAACAAGGAA AGGCGTTGCT TATCCAAGAG 
TCAAAATGGA AATTGCCACA CCTTCTACAG TTGCCTGAAA ATTATAACAC CATTTTCCAG 
TACTACCACA GAAAAACCTG TAGTGTCTGC ACCAAGGTTC CTAAAGACCC TGCCGTTTGC 
CTTGTTTGTG GTACTTTTGT ATGCCTGAAA GGACTTTGCT GCAAGCAACA AAGTTACTGT 
GAATGTGTAT TGCATTCTCA GAACTGTGGT GCTGGAACAG GGATTTTCCT TTTGATCAAT 
GCATCAGTGA TTATCATCAT TCGAGGCCAC CGCTTCTGCC TCTGGGGTTC TGTGTATTTG 
GATGCTCATG GAGAGGAAGA CCGAGATCTT AGGCGAGGCA AGCCTCTCTA CATTTGTAAA 
GAAAGATACA AAGTTCTTGA ACAACAGTGG ATTTCTCATA CTTTTGATCA CATCAATAAA 
AGATGGGGTC CACATTACAA TGGGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800236.1
CDS	49..5718
Translation	MAAAAAAAVGGPQPPQAEVPAPGLALDKAATAAHLKAALSRPDNRAGAEELQALLERVLSAERPLAAAADGEEAAAAGGGGGPGAAEEEALEWCKCLLAGGGGYDEFCAAVRAYDPAALCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGDHTGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHQIKSSSNIPCVPKDLLMMSEFVLPRFIFCLIQYLREGYNEPAADVPSEKDLNKVLQLLEPQISFLEDLTKMGGAMRSVLTQVLTNQQNYKDLTSGLGENACARKSHEKYLIALKSSGLTYPEDKLVYGIQEPSAGASTLAVQGFASAAGTLGQLDSSDEEDQDGSQGLGKRKRVKLSSNTKDQSIMDVLKHKSFLEELLFWTIKYEFPQKMVTFLLNMLPDQEYKVAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHGLLVTWMNFVSFFQGMNLNKRELNEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVRETQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAVKCQELDLDSVLPDQEMLMKLMIHPLQIQASLAEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNTVLDAEHERSMLEGALTFLVILLSLRLHLGMSDDEILRAEMVAQLCMNDRTHSSLLDLIPENPNPKSGIIPGSYSFESVLSAVADFKAPVFEPGGSMQQGMYTPKAEVWDHEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGKFPGNPWPPYKKRTPLHPSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNLSEHVLCMVLYLIELGLENSAEEESDEEASACGPERCHDSWFPGSNLVSNMRHFINYVRVRVPETAPEVKRDSLASTSSDSLGSLQNSGTAQVFSLVAERRKKFQEIINRSNSEANQVVRPKTSSKWSAPGSAPQLTTAILEIKESILSLLIKLHHKLSGKQNSYYPPWLDDIEILIQPEIPKYSHGDGITAVERILLKAALQSRMNKRIIEEICRKVTPPVPPKKITAAEKKTLDKEERRQKARERQQKLLAEFASRQKSFMETAMDVDSPESDIPMEITTAEPQVSEAVYDCVICGQSGPSSEDRPTGLVVLLQASSVLGQCRDNIEPKKLPISEEEQIYPWDTCAAVHDVRLSLLQRYFKDSSCLLAVSIGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCYPGSNVENHLWQRPSNKSIQDLIKEVEELQGRPGAFPSETNLSKEMESVMKDIKNTTQKKYRDYSKTPGSPDNDFLFMYSVARTNLELELIHRGGNLCSGGASTAGKRSCLNQLFHVLALHMRLYSIDSEYNPWRKLTQLLEEMSSQLGNEEQQPEVPILYHDVTSLLLIQILMMPQPLHKDHFTCIVKVLFTLLYTQGLVALSVKCNEEDRSAWKHTGALKKNTCDAEKSYEVLLSFVISELSKGKLYHEEGTQECATIIPVAWSPESMEKYLQDFCLPFLRITSLLQHHLFGEDLPSCQEEEEFSVLSSCLGLLPTFYQTEHPFISASCLDWPVPAFDIISQWCFEINSFTERHAEQGKALLIQESKWKLPHLLQLPENYNTIFQYYHRKTCSVCTKVPKDPAVCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYKVLEQQWISHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_010801934.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801934.3

ORF Insert Sequence:

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAATTCT GGTACAGCTC AGGTTTTCAG CTTAGTAGCA 
GAGCGCAGAA AGAAGTTTCA AGAGATCATC AATCGCAGTA ACAGTGAAGC AAATCAAGTG 
GTTCGTCCTA AAACTTCAAG TAAATGGTCT GCACCTGGTT CAGCTCCACA GTTAACTACA 
GCCATTTTGG AAATTAAAGA AAGTATATTG TCTTTGTTAA TTAAACTTCA CCACAAACTC 
TCAGGAAAAC AAAATTCCTA CTATCCTCCT TGGCTTGATG ACATAGAAAT TTTAATCCAA 
CCAGAAATTC CTAAATATAG TCATGGAGAT GGTATAACTG CAGTAGAAAG AATTTTACTA 
AAAGCTGCAT TGCAAAGCAG AATGAACAAA CGCATCATTG AAGAGATATG TAGAAAAGTG 
ACCCCTCCTG TACCACCCAA AAAGATTACT GCAGCAGAGA AGAAAACACT GGACAAAGAA 
GAAAGGCGAC AAAAAGCTAG AGAAAGACAG CAGAAATTGC TTGCAGAGTT TGCATCACGA 
CAGAAAAGCT TCATGGAAAC TGCCATGGAT GTTGATTCTC CAGAGAGTGA TATTCCTATG 
GAGATCACCA CAGCAGAACC TCAAGTTTCT GAGGCAGTAT ATGATTGTGT TATTTGTGGA 
CAGAGTGGGC CCTCCTCTGA AGACAGACCT ACGGGATTGG TTGTCCTGTT ACAAGCATCC 
TCAGTTTTGG GGCAGTGCCG TGACAACATT GAGCCAAAAA AGTTGCCTAT CTCTGAAGAG 
GAGCAGATAT ACCCCTGGGA TACATGTGCA GCAGTTCATG ACGTGAGGCT CTCATTATTA 
CAACGTTATT TTAAGGATAG TTCTTGTCTC TTGGCAGTAT CAATTGGCTG GGAAGGAGGT 
GTTTATGTAC AAACCTGTGG CCACACATTA CATATCGATT GCCATAAATC TTACATGGAA 
TCATTACGGA ATGACCAGGT TCTTCAGGGC TTCTCAGTGG ACAAAGGAGA ATTCACATGT 
CCTCTCTGTA GGCAGTTTGC TAACAGTGTT CTTCCTTGTT ATCCTGGAAG CAATGTTGAA 
AACCACCTTT GGCAGCGTCC TAGTAACAAA AGCATACAAG ATCTCATAAA GGAAGTGGAG 
GAGCTGCAAG GACGTCCGGG AGCTTTCCCA TCAGAAACCA ACTTAAGTAA AGAAATGGAA 
TCTGTAATGA AAGATATAAA AAATACCACT CAGAAGAAAT ACAGAGATTA TAGCAAGACT 
CCAGGCTCAC CAGATAATGA TTTTCTCTTT ATGTATTCTG TTGCTAGAAC CAACTTGGAA 
CTTGAATTAA TTCATCGAGG AGGGAATTTA TGTTCAGGTG GTGCAAGTAC AGCTGGTAAA 
AGGTCCTGTT TAAATCAGCT GTTCCATGTA TTAGCCTTGC ACATGCGACT TTATAGCATT 
GATTCTGAGT ATAATCCTTG GAGAAAGCTC ACACAGTTAC TAGAAGAGAT GAGTTCGCAA 
CTGGGAAATG AGGAACAACA GCCTGAGGTT CCAATTCTTT ATCATGATGT TACATCCCTT 
TTGCTCATCC AGATCTTAAT GATGCCTCAA CCCTTACACA AAGACCACTT CACCTGCATT 
GTGAAGGTGC TTTTTACCCT ACTGTATACA CAAGGTCTTG TGGCACTTTC AGTTAAATGC 
AATGAGGAAG ATAGGTCAGC CTGGAAACAC ACAGGAGCCC TCAAAAAGAA TACTTGTGAT 
GCAGAAAAGT CTTATGAAGT GTTATTGAGC TTTGTGATAA GTGAACTGTC TAAAGGAAAG 
TTATACCATG AAGAAGGAAC TCAGGAGTGT GCAACGATTA TCCCTGTTGC TTGGTCTCCT 
GAATCCATGG AAAAATACTT ACAGGACTTC TGCTTACCAT TTCTCAGAAT CACCAGCCTT 
CTTCAGCACC ACCTTTTTGG GGAAGATTTA CCTAGCTGCC AGGAAGAAGA AGAATTTTCA 
GTTCTTTCCA GCTGCCTGGG ACTTCTGCCA ACATTCTACC AAACAGAACA TCCATTCATC 
AGTGCCTCCT GTCTGGATTG GCCAGTTCCG GCATTTGATA TTATATCTCA GTGGTGTTTT 
GAGATAAATT CATTTACTGA AAGACATGCT GAACAAGGAA AGGCGTTGCT TATCCAAGAG 
TCAAAATGGA AATTGCCACA CCTTCTACAG TTGCCTGAAA ATTATAACAC CATTTTCCAG 
TACTACCACA GAAAAACCTG TAGTGTCTGC ACCAAGGTTC CTAAAGACCC TGCCGTTTGC 
CTTGTTTGTG GTACTTTTGT ATGCCTGAAA GGACTTTGCT GCAAGCAACA AAGTTACTGT 
GAATGTGTAT TGCATTCTCA GAACTGTGGT GCTGGAACAG GGATTTTCCT TTTGATCAAT 
GCATCAGTGA TTATCATCAT TCGAGGCCAC CGCTTCTGCC TCTGGGGTTC TGTGTATTTG 
GATGCTCATG GAGAGGAAGA CCGAGATCTT AGGCGAGGCA AGCCTCTCTA CATTTGTAAA 
GAAAGATACA AAGTTCTTGA ACAACAGTGG ATTTCTCATA CTTTTGATCA CATCAATAAA 
AGATGGGGTC CACATTACAA TGGGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR3 ( XM_015462262.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317748.1 Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X5, mRNA.

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CloneID	OBa53814
Clone ID Related Accession (Same CDS sequence)	XM_015462262.1 , XM_010801935.3 , XM_010801935.2
Accession Version	XM_015462262.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase UBR3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644584.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAGTTCA CGTCGGCCTA AAGTTCTTCA AAGAGAGAAT 
TCTGGTACAG CTCAGGTTTT CAGCTTAGTA GCAGAGCGCA GAAAGAAGTT TCAAGAGATC 
ATCAATCGCA GTAACAGTGA AGCAAATCAA GTGGTTCGTC CTAAAACTTC AAGTAAATGG 
TCTGCACCTG GTTCAGCTCC ACAGTTAACT ACAGCCATTT TGGAAATTAA AGAAAGTATA 
TTGTCTTTGT TAATTAAACT TCACCACAAA CTCTCAGGAA AACAAAATTC CTACTATCCT 
CCTTGGCTTG ATGACATAGA AATTTTAATC CAACCAGAAA TTCCTAAATA TAGTCATGGA 
GATGGTATAA CTGCAGTAGA AAGAATTTTA CTAAAAGCTG CATTGCAAAG CAGAATGAAC 
AAACGCATCA TTGAAGAGAT ATGTAGAAAA GTGACCCCTC CTGTACCACC CAAAAAGATT 
ACTGCAGCAG AGAAGAAAAC ACTGGACAAA GAAGAAAGGC GACAAAAAGC TAGAGAAAGA 
CAGCAGAAAT TGCTTGCAGA GTTTGCATCA CGACAGAAAA GCTTCATGGA AACTGCCATG 
GATGTTGATT CTCCAGAGAG TGATATTCCT ATGGAGATCA CCACAGCAGA ACCTCAAGTT 
TCTGAGGCAG TATATGATTG TGTTATTTGT GGACAGAGTG GGCCCTCCTC TGAAGACAGA 
CCTACGGGAT TGGTTGTCCT GTTACAAGCA TCCTCAGTTT TGGGGCAGTG CCGTGACAAC 
ATTGAGCCAA AAAAGTTGCC TATCTCTGAA GAGGAGCAGA TATACCCCTG GGATACATGT 
GCAGCAGTTC ATGACGTGAG GCTCTCATTA TTACAACGTT ATTTTAAGGA TAGTTCTTGT 
CTCTTGGCAG TATCAATTGG CTGGGAAGGA GGTGTTTATG TACAAACCTG TGGCCACACA 
TTACATATCG ATTGCCATAA ATCTTACATG GAATCATTAC GGAATGACCA GGTTCTTCAG 
GGCTTCTCAG TGGACAAAGG AGAATTCACA TGTCCTCTCT GTAGGCAGTT TGCTAACAGT 
GTTCTTCCTT GTTATCCTGG AAGCAATGTT GAAAACCACC TTTGGCAGCG TCCTAGTAAC 
AAAAGCATAC AAGATCTCAT AAAGGAAGTG GAGGAGCTGC AAGGACGTCC GGGAGCTTTC 
CCAGTAAGCA TCAGTTCAGA AACCAACTTA AGTAAAGAAA TGGAATCTGT AATGAAAGAT 
ATAAAAAATA CCACTCAGAA GAAATACAGA GATTATAGCA AGACTCCAGG CTCACCAGAT 
AATGATTTTC TCTTTATGTA TTCTGTTGCT AGAACCAACT TGGAACTTGA ATTAATTCAT 
CGAGGAGGGA ATTTATGTTC AGGTGGTGCA AGTACAGCTG GTAAAAGGTC CTGTTTAAAA 
AAGGAAACTG AACTTCAGAG TGATTGCATT GCCAAGTCAT ACAGCGGAAA ATGTCAAAGC 
TTGCAGTTGA ACTCAGCTCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317748.1
CDS	47..4630
Translation	MAAAAAAAVGGPQPPQAEVPAPGLALDKAATAAHLKAALSRPDNRAGAEELQALLERVLSAERPLAAAADGEEAAAAGGGGGPGAAEEEALEWCKCLLAGGGGYDEFCAAVRAYDPAALCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGDHTGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHQIKSSSNIPCVPKDLLMMSEFVLPRFIFCLIQYLREGYNEPAADVPSEKDLNKVLQLLEPQISFLEDLTKMGGAMRSVLTQVLTNQQNYKDLTSGLGENACARKSHEKYLIALKSSGLTYPEDKLVYGIQEPSAGASTLAVQGFASAAGTLGQLDSSDEEDQDGSQGLGKRKRVKLSSNTKDQSIMDVLKHKSFLEELLFWTIKYEFPQKMVTFLLNMLPDQEYKVAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHGLLVTWMNFVSFFQGMNLNKRELNEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVRETQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAVKCQELDLDSVLPDQEMLMKLMIHPLQIQASLAEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNTVLDAEHERSMLEGALTFLVILLSLRLHLGMSDDEILRAEMVAQLCMNDRTHSSLLDLIPENPNPKSGIIPGSYSFESVLSAVADFKAPVFEPGGSMQQGMYTPKAEVWDHEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGKFPGNPWPPYKKRTPLHPSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNLSEHVLCMVLYLIELGLENSAEEESDEEASACGPERCHDSWFPGSNLVSNMRHFINYVRVRVPETAPEVKRDSLASTSSDSLGSLQSSRRPKVLQRENSGTAQVFSLVAERRKKFQEIINRSNSEANQVVRPKTSSKWSAPGSAPQLTTAILEIKESILSLLIKLHHKLSGKQNSYYPPWLDDIEILIQPEIPKYSHGDGITAVERILLKAALQSRMNKRIIEEICRKVTPPVPPKKITAAEKKTLDKEERRQKARERQQKLLAEFASRQKSFMETAMDVDSPESDIPMEITTAEPQVSEAVYDCVICGQSGPSSEDRPTGLVVLLQASSVLGQCRDNIEPKKLPISEEEQIYPWDTCAAVHDVRLSLLQRYFKDSSCLLAVSIGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCYPGSNVENHLWQRPSNKSIQDLIKEVEELQGRPGAFPVSISSETNLSKEMESVMKDIKNTTQKKYRDYSKTPGSPDNDFLFMYSVARTNLELELIHRGGNLCSGGASTAGKRSCLKKETELQSDCIAKSYSGKCQSLQLNSAL

Target ORF information:

RefSeq Version	XM_015462262.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462262.1

ORF Insert Sequence:

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAGTTCA CGTCGGCCTA AAGTTCTTCA AAGAGAGAAT 
TCTGGTACAG CTCAGGTTTT CAGCTTAGTA GCAGAGCGCA GAAAGAAGTT TCAAGAGATC 
ATCAATCGCA GTAACAGTGA AGCAAATCAA GTGGTTCGTC CTAAAACTTC AAGTAAATGG 
TCTGCACCTG GTTCAGCTCC ACAGTTAACT ACAGCCATTT TGGAAATTAA AGAAAGTATA 
TTGTCTTTGT TAATTAAACT TCACCACAAA CTCTCAGGAA AACAAAATTC CTACTATCCT 
CCTTGGCTTG ATGACATAGA AATTTTAATC CAACCAGAAA TTCCTAAATA TAGTCATGGA 
GATGGTATAA CTGCAGTAGA AAGAATTTTA CTAAAAGCTG CATTGCAAAG CAGAATGAAC 
AAACGCATCA TTGAAGAGAT ATGTAGAAAA GTGACCCCTC CTGTACCACC CAAAAAGATT 
ACTGCAGCAG AGAAGAAAAC ACTGGACAAA GAAGAAAGGC GACAAAAAGC TAGAGAAAGA 
CAGCAGAAAT TGCTTGCAGA GTTTGCATCA CGACAGAAAA GCTTCATGGA AACTGCCATG 
GATGTTGATT CTCCAGAGAG TGATATTCCT ATGGAGATCA CCACAGCAGA ACCTCAAGTT 
TCTGAGGCAG TATATGATTG TGTTATTTGT GGACAGAGTG GGCCCTCCTC TGAAGACAGA 
CCTACGGGAT TGGTTGTCCT GTTACAAGCA TCCTCAGTTT TGGGGCAGTG CCGTGACAAC 
ATTGAGCCAA AAAAGTTGCC TATCTCTGAA GAGGAGCAGA TATACCCCTG GGATACATGT 
GCAGCAGTTC ATGACGTGAG GCTCTCATTA TTACAACGTT ATTTTAAGGA TAGTTCTTGT 
CTCTTGGCAG TATCAATTGG CTGGGAAGGA GGTGTTTATG TACAAACCTG TGGCCACACA 
TTACATATCG ATTGCCATAA ATCTTACATG GAATCATTAC GGAATGACCA GGTTCTTCAG 
GGCTTCTCAG TGGACAAAGG AGAATTCACA TGTCCTCTCT GTAGGCAGTT TGCTAACAGT 
GTTCTTCCTT GTTATCCTGG AAGCAATGTT GAAAACCACC TTTGGCAGCG TCCTAGTAAC 
AAAAGCATAC AAGATCTCAT AAAGGAAGTG GAGGAGCTGC AAGGACGTCC GGGAGCTTTC 
CCAGTAAGCA TCAGTTCAGA AACCAACTTA AGTAAAGAAA TGGAATCTGT AATGAAAGAT 
ATAAAAAATA CCACTCAGAA GAAATACAGA GATTATAGCA AGACTCCAGG CTCACCAGAT 
AATGATTTTC TCTTTATGTA TTCTGTTGCT AGAACCAACT TGGAACTTGA ATTAATTCAT 
CGAGGAGGGA ATTTATGTTC AGGTGGTGCA AGTACAGCTG GTAAAAGGTC CTGTTTAAAA 
AAGGAAACTG AACTTCAGAG TGATTGCATT GCCAAGTCAT ACAGCGGAAA ATGTCAAAGC 
TTGCAGTTGA ACTCAGCTCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR3 ( XM_010801935.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800237.1 Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X6, mRNA.

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CloneID	OBa53814
Clone ID Related Accession (Same CDS sequence)	XM_015462262.1 , XM_010801935.3 , XM_010801935.2
Accession Version	XM_010801935.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase UBR3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801935.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAGTTCA CGTCGGCCTA AAGTTCTTCA AAGAGAGAAT 
TCTGGTACAG CTCAGGTTTT CAGCTTAGTA GCAGAGCGCA GAAAGAAGTT TCAAGAGATC 
ATCAATCGCA GTAACAGTGA AGCAAATCAA GTGGTTCGTC CTAAAACTTC AAGTAAATGG 
TCTGCACCTG GTTCAGCTCC ACAGTTAACT ACAGCCATTT TGGAAATTAA AGAAAGTATA 
TTGTCTTTGT TAATTAAACT TCACCACAAA CTCTCAGGAA AACAAAATTC CTACTATCCT 
CCTTGGCTTG ATGACATAGA AATTTTAATC CAACCAGAAA TTCCTAAATA TAGTCATGGA 
GATGGTATAA CTGCAGTAGA AAGAATTTTA CTAAAAGCTG CATTGCAAAG CAGAATGAAC 
AAACGCATCA TTGAAGAGAT ATGTAGAAAA GTGACCCCTC CTGTACCACC CAAAAAGATT 
ACTGCAGCAG AGAAGAAAAC ACTGGACAAA GAAGAAAGGC GACAAAAAGC TAGAGAAAGA 
CAGCAGAAAT TGCTTGCAGA GTTTGCATCA CGACAGAAAA GCTTCATGGA AACTGCCATG 
GATGTTGATT CTCCAGAGAG TGATATTCCT ATGGAGATCA CCACAGCAGA ACCTCAAGTT 
TCTGAGGCAG TATATGATTG TGTTATTTGT GGACAGAGTG GGCCCTCCTC TGAAGACAGA 
CCTACGGGAT TGGTTGTCCT GTTACAAGCA TCCTCAGTTT TGGGGCAGTG CCGTGACAAC 
ATTGAGCCAA AAAAGTTGCC TATCTCTGAA GAGGAGCAGA TATACCCCTG GGATACATGT 
GCAGCAGTTC ATGACGTGAG GCTCTCATTA TTACAACGTT ATTTTAAGGA TAGTTCTTGT 
CTCTTGGCAG TATCAATTGG CTGGGAAGGA GGTGTTTATG TACAAACCTG TGGCCACACA 
TTACATATCG ATTGCCATAA ATCTTACATG GAATCATTAC GGAATGACCA GGTTCTTCAG 
GGCTTCTCAG TGGACAAAGG AGAATTCACA TGTCCTCTCT GTAGGCAGTT TGCTAACAGT 
GTTCTTCCTT GTTATCCTGG AAGCAATGTT GAAAACCACC TTTGGCAGCG TCCTAGTAAC 
AAAAGCATAC AAGATCTCAT AAAGGAAGTG GAGGAGCTGC AAGGACGTCC GGGAGCTTTC 
CCAGTAAGCA TCAGTTCAGA AACCAACTTA AGTAAAGAAA TGGAATCTGT AATGAAAGAT 
ATAAAAAATA CCACTCAGAA GAAATACAGA GATTATAGCA AGACTCCAGG CTCACCAGAT 
AATGATTTTC TCTTTATGTA TTCTGTTGCT AGAACCAACT TGGAACTTGA ATTAATTCAT 
CGAGGAGGGA ATTTATGTTC AGGTGGTGCA AGTACAGCTG GTAAAAGGTC CTGTTTAAAA 
AAGGAAACTG AACTTCAGAG TGATTGCATT GCCAAGTCAT ACAGCGGAAA ATGTCAAAGC 
TTGCAGTTGA ACTCAGCTCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800237.1
CDS	53..4636
Translation	MAAAAAAAVGGPQPPQAEVPAPGLALDKAATAAHLKAALSRPDNRAGAEELQALLERVLSAERPLAAAADGEEAAAAGGGGGPGAAEEEALEWCKCLLAGGGGYDEFCAAVRAYDPAALCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGDHTGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHQIKSSSNIPCVPKDLLMMSEFVLPRFIFCLIQYLREGYNEPAADVPSEKDLNKVLQLLEPQISFLEDLTKMGGAMRSVLTQVLTNQQNYKDLTSGLGENACARKSHEKYLIALKSSGLTYPEDKLVYGIQEPSAGASTLAVQGFASAAGTLGQLDSSDEEDQDGSQGLGKRKRVKLSSNTKDQSIMDVLKHKSFLEELLFWTIKYEFPQKMVTFLLNMLPDQEYKVAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHGLLVTWMNFVSFFQGMNLNKRELNEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVRETQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAVKCQELDLDSVLPDQEMLMKLMIHPLQIQASLAEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNTVLDAEHERSMLEGALTFLVILLSLRLHLGMSDDEILRAEMVAQLCMNDRTHSSLLDLIPENPNPKSGIIPGSYSFESVLSAVADFKAPVFEPGGSMQQGMYTPKAEVWDHEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGKFPGNPWPPYKKRTPLHPSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNLSEHVLCMVLYLIELGLENSAEEESDEEASACGPERCHDSWFPGSNLVSNMRHFINYVRVRVPETAPEVKRDSLASTSSDSLGSLQSSRRPKVLQRENSGTAQVFSLVAERRKKFQEIINRSNSEANQVVRPKTSSKWSAPGSAPQLTTAILEIKESILSLLIKLHHKLSGKQNSYYPPWLDDIEILIQPEIPKYSHGDGITAVERILLKAALQSRMNKRIIEEICRKVTPPVPPKKITAAEKKTLDKEERRQKARERQQKLLAEFASRQKSFMETAMDVDSPESDIPMEITTAEPQVSEAVYDCVICGQSGPSSEDRPTGLVVLLQASSVLGQCRDNIEPKKLPISEEEQIYPWDTCAAVHDVRLSLLQRYFKDSSCLLAVSIGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCYPGSNVENHLWQRPSNKSIQDLIKEVEELQGRPGAFPVSISSETNLSKEMESVMKDIKNTTQKKYRDYSKTPGSPDNDFLFMYSVARTNLELELIHRGGNLCSGGASTAGKRSCLKKETELQSDCIAKSYSGKCQSLQLNSAL

Target ORF information:

RefSeq Version	XM_010801935.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801935.3

ORF Insert Sequence:

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAGTTCA CGTCGGCCTA AAGTTCTTCA AAGAGAGAAT 
TCTGGTACAG CTCAGGTTTT CAGCTTAGTA GCAGAGCGCA GAAAGAAGTT TCAAGAGATC 
ATCAATCGCA GTAACAGTGA AGCAAATCAA GTGGTTCGTC CTAAAACTTC AAGTAAATGG 
TCTGCACCTG GTTCAGCTCC ACAGTTAACT ACAGCCATTT TGGAAATTAA AGAAAGTATA 
TTGTCTTTGT TAATTAAACT TCACCACAAA CTCTCAGGAA AACAAAATTC CTACTATCCT 
CCTTGGCTTG ATGACATAGA AATTTTAATC CAACCAGAAA TTCCTAAATA TAGTCATGGA 
GATGGTATAA CTGCAGTAGA AAGAATTTTA CTAAAAGCTG CATTGCAAAG CAGAATGAAC 
AAACGCATCA TTGAAGAGAT ATGTAGAAAA GTGACCCCTC CTGTACCACC CAAAAAGATT 
ACTGCAGCAG AGAAGAAAAC ACTGGACAAA GAAGAAAGGC GACAAAAAGC TAGAGAAAGA 
CAGCAGAAAT TGCTTGCAGA GTTTGCATCA CGACAGAAAA GCTTCATGGA AACTGCCATG 
GATGTTGATT CTCCAGAGAG TGATATTCCT ATGGAGATCA CCACAGCAGA ACCTCAAGTT 
TCTGAGGCAG TATATGATTG TGTTATTTGT GGACAGAGTG GGCCCTCCTC TGAAGACAGA 
CCTACGGGAT TGGTTGTCCT GTTACAAGCA TCCTCAGTTT TGGGGCAGTG CCGTGACAAC 
ATTGAGCCAA AAAAGTTGCC TATCTCTGAA GAGGAGCAGA TATACCCCTG GGATACATGT 
GCAGCAGTTC ATGACGTGAG GCTCTCATTA TTACAACGTT ATTTTAAGGA TAGTTCTTGT 
CTCTTGGCAG TATCAATTGG CTGGGAAGGA GGTGTTTATG TACAAACCTG TGGCCACACA 
TTACATATCG ATTGCCATAA ATCTTACATG GAATCATTAC GGAATGACCA GGTTCTTCAG 
GGCTTCTCAG TGGACAAAGG AGAATTCACA TGTCCTCTCT GTAGGCAGTT TGCTAACAGT 
GTTCTTCCTT GTTATCCTGG AAGCAATGTT GAAAACCACC TTTGGCAGCG TCCTAGTAAC 
AAAAGCATAC AAGATCTCAT AAAGGAAGTG GAGGAGCTGC AAGGACGTCC GGGAGCTTTC 
CCAGTAAGCA TCAGTTCAGA AACCAACTTA AGTAAAGAAA TGGAATCTGT AATGAAAGAT 
ATAAAAAATA CCACTCAGAA GAAATACAGA GATTATAGCA AGACTCCAGG CTCACCAGAT 
AATGATTTTC TCTTTATGTA TTCTGTTGCT AGAACCAACT TGGAACTTGA ATTAATTCAT 
CGAGGAGGGA ATTTATGTTC AGGTGGTGCA AGTACAGCTG GTAAAAGGTC CTGTTTAAAA 
AAGGAAACTG AACTTCAGAG TGATTGCATT GCCAAGTCAT ACAGCGGAAA ATGTCAAAGC 
TTGCAGTTGA ACTCAGCTCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR3 ( XM_010801935.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800237.1 Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X5, mRNA.

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CloneID	OBa53814
Clone ID Related Accession (Same CDS sequence)	XM_015462262.1 , XM_010801935.3 , XM_010801935.2
Accession Version	XM_010801935.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase UBR3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801935.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAGTTCA CGTCGGCCTA AAGTTCTTCA AAGAGAGAAT 
TCTGGTACAG CTCAGGTTTT CAGCTTAGTA GCAGAGCGCA GAAAGAAGTT TCAAGAGATC 
ATCAATCGCA GTAACAGTGA AGCAAATCAA GTGGTTCGTC CTAAAACTTC AAGTAAATGG 
TCTGCACCTG GTTCAGCTCC ACAGTTAACT ACAGCCATTT TGGAAATTAA AGAAAGTATA 
TTGTCTTTGT TAATTAAACT TCACCACAAA CTCTCAGGAA AACAAAATTC CTACTATCCT 
CCTTGGCTTG ATGACATAGA AATTTTAATC CAACCAGAAA TTCCTAAATA TAGTCATGGA 
GATGGTATAA CTGCAGTAGA AAGAATTTTA CTAAAAGCTG CATTGCAAAG CAGAATGAAC 
AAACGCATCA TTGAAGAGAT ATGTAGAAAA GTGACCCCTC CTGTACCACC CAAAAAGATT 
ACTGCAGCAG AGAAGAAAAC ACTGGACAAA GAAGAAAGGC GACAAAAAGC TAGAGAAAGA 
CAGCAGAAAT TGCTTGCAGA GTTTGCATCA CGACAGAAAA GCTTCATGGA AACTGCCATG 
GATGTTGATT CTCCAGAGAG TGATATTCCT ATGGAGATCA CCACAGCAGA ACCTCAAGTT 
TCTGAGGCAG TATATGATTG TGTTATTTGT GGACAGAGTG GGCCCTCCTC TGAAGACAGA 
CCTACGGGAT TGGTTGTCCT GTTACAAGCA TCCTCAGTTT TGGGGCAGTG CCGTGACAAC 
ATTGAGCCAA AAAAGTTGCC TATCTCTGAA GAGGAGCAGA TATACCCCTG GGATACATGT 
GCAGCAGTTC ATGACGTGAG GCTCTCATTA TTACAACGTT ATTTTAAGGA TAGTTCTTGT 
CTCTTGGCAG TATCAATTGG CTGGGAAGGA GGTGTTTATG TACAAACCTG TGGCCACACA 
TTACATATCG ATTGCCATAA ATCTTACATG GAATCATTAC GGAATGACCA GGTTCTTCAG 
GGCTTCTCAG TGGACAAAGG AGAATTCACA TGTCCTCTCT GTAGGCAGTT TGCTAACAGT 
GTTCTTCCTT GTTATCCTGG AAGCAATGTT GAAAACCACC TTTGGCAGCG TCCTAGTAAC 
AAAAGCATAC AAGATCTCAT AAAGGAAGTG GAGGAGCTGC AAGGACGTCC GGGAGCTTTC 
CCAGTAAGCA TCAGTTCAGA AACCAACTTA AGTAAAGAAA TGGAATCTGT AATGAAAGAT 
ATAAAAAATA CCACTCAGAA GAAATACAGA GATTATAGCA AGACTCCAGG CTCACCAGAT 
AATGATTTTC TCTTTATGTA TTCTGTTGCT AGAACCAACT TGGAACTTGA ATTAATTCAT 
CGAGGAGGGA ATTTATGTTC AGGTGGTGCA AGTACAGCTG GTAAAAGGTC CTGTTTAAAA 
AAGGAAACTG AACTTCAGAG TGATTGCATT GCCAAGTCAT ACAGCGGAAA ATGTCAAAGC 
TTGCAGTTGA ACTCAGCTCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800237.1
CDS	47..4630
Translation	MAAAAAAAVGGPQPPQAEVPAPGLALDKAATAAHLKAALSRPDNRAGAEELQALLERVLSAERPLAAAADGEEAAAAGGGGGPGAAEEEALEWCKCLLAGGGGYDEFCAAVRAYDPAALCGLVWTANFVAYRCRTCGISPCMSLCAECFHQGDHTGHDFNMFRSQAGGACDCGDSNVMRESGFCKRHQIKSSSNIPCVPKDLLMMSEFVLPRFIFCLIQYLREGYNEPAADVPSEKDLNKVLQLLEPQISFLEDLTKMGGAMRSVLTQVLTNQQNYKDLTSGLGENACARKSHEKYLIALKSSGLTYPEDKLVYGIQEPSAGASTLAVQGFASAAGTLGQLDSSDEEDQDGSQGLGKRKRVKLSSNTKDQSIMDVLKHKSFLEELLFWTIKYEFPQKMVTFLLNMLPDQEYKVAFTKTFVQHYAFIMKTLKKSHESDTMSNRIVHISVQLFSNEELARQVTEECQLLDIMVTVLLYMMESCLIKSELQDEENSLHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHGLLVTWMNFVSFFQGMNLNKRELNEHVEFESQTYYAAFAAELEACAQPMWGLLSHCKVRETQEYTRNVVRYCLEALQDWFDAINFVDEPAPNQVTFHLPLHRYYAMFLSKAVKCQELDLDSVLPDQEMLMKLMIHPLQIQASLAEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNTVLDAEHERSMLEGALTFLVILLSLRLHLGMSDDEILRAEMVAQLCMNDRTHSSLLDLIPENPNPKSGIIPGSYSFESVLSAVADFKAPVFEPGGSMQQGMYTPKAEVWDHEFDPVMVILRTVYRRDVQSAMDRYTAFLKQSGKFPGNPWPPYKKRTPLHPSYKGLMRLLHCKTLHIVLFTLLYKILMDHQNLSEHVLCMVLYLIELGLENSAEEESDEEASACGPERCHDSWFPGSNLVSNMRHFINYVRVRVPETAPEVKRDSLASTSSDSLGSLQSSRRPKVLQRENSGTAQVFSLVAERRKKFQEIINRSNSEANQVVRPKTSSKWSAPGSAPQLTTAILEIKESILSLLIKLHHKLSGKQNSYYPPWLDDIEILIQPEIPKYSHGDGITAVERILLKAALQSRMNKRIIEEICRKVTPPVPPKKITAAEKKTLDKEERRQKARERQQKLLAEFASRQKSFMETAMDVDSPESDIPMEITTAEPQVSEAVYDCVICGQSGPSSEDRPTGLVVLLQASSVLGQCRDNIEPKKLPISEEEQIYPWDTCAAVHDVRLSLLQRYFKDSSCLLAVSIGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCYPGSNVENHLWQRPSNKSIQDLIKEVEELQGRPGAFPVSISSETNLSKEMESVMKDIKNTTQKKYRDYSKTPGSPDNDFLFMYSVARTNLELELIHRGGNLCSGGASTAGKRSCLKKETELQSDCIAKSYSGKCQSLQLNSAL

Target ORF information:

RefSeq Version	XM_010801935.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin protein ligase E3 component n-recognin 3 (putative) (UBR3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801935.2

ORF Insert Sequence:

ATGGCGGCGG CGGCCGCTGC GGCCGTCGGG GGGCCGCAGC CACCCCAAGC CGAGGTGCCT 
GCTCCCGGGC TGGCCCTGGA CAAAGCAGCC ACCGCCGCGC ACCTGAAAGC GGCCCTCAGC 
CGGCCGGACA ACCGGGCAGG TGCTGAGGAG CTGCAGGCGC TGCTGGAGCG GGTGCTGAGC 
GCCGAGCGGC CGCTGGCAGC GGCTGCGGAC GGCGAGGAGG CGGCGGCGGC CGGCGGCGGG 
GGCGGTCCGG GGGCGGCCGA GGAGGAGGCT CTGGAGTGGT GTAAGTGCCT TCTGGCGGGC 
GGCGGAGGCT ACGACGAGTT CTGCGCGGCG GTGCGGGCCT ACGACCCCGC GGCGCTATGC 
GGCCTGGTCT GGACTGCCAA CTTCGTGGCC TACCGCTGCC GGACGTGCGG CATCTCCCCC 
TGCATGTCGC TTTGCGCCGA GTGCTTCCAC CAGGGTGACC ACACCGGACA CGACTTCAAC 
ATGTTCCGTA GCCAGGCCGG GGGTGCCTGC GACTGCGGAG ACAGCAACGT GATGCGGGAG 
AGTGGGTTTT GCAAAAGGCA TCAAATTAAA TCAAGTTCAA ATATTCCTTG TGTCCCCAAA 
GACTTACTGA TGATGTCTGA ATTTGTTCTT CCAAGATTTA TTTTTTGTCT TATTCAGTAT 
TTAAGAGAAG GCTATAATGA ACCAGCAGCT GATGTTCCAT CAGAGAAAGA CCTTAATAAA 
GTCCTTCAGC TTTTGGAACC TCAAATTTCC TTTTTAGAAG ATCTAACTAA AATGGGAGGA 
GCAATGCGAT CTGTTCTTAC TCAGGTTTTA ACAAACCAAC AAAACTACAA AGATCTGACT 
TCTGGTCTTG GAGAAAATGC TTGTGCAAGG AAGAGTCATG AAAAATACCT TATAGCTTTG 
AAGAGCTCTG GACTTACATA TCCTGAGGAT AAACTTGTAT ACGGTATACA AGAGCCATCT 
GCTGGCGCTA GCACTCTGGC AGTTCAAGGT TTTGCAAGTG CAGCAGGAAC ATTGGGACAA 
CTAGATTCTT CAGATGAGGA GGATCAGGAT GGGAGTCAAG GTCTGGGCAA AAGAAAGAGG 
GTCAAACTAA GCAGTAACAC CAAAGATCAA TCCATAATGG ATGTTTTGAA ACATAAAAGT 
TTCTTAGAAG AGCTATTGTT TTGGACAATA AAGTATGAAT TTCCGCAGAA GATGGTGACT 
TTCTTACTCA ACATGCTTCC AGATCAAGAG TATAAGGTTG CTTTTACAAA AACTTTTGTT 
CAGCATTATG CTTTCATTAT GAAGACACTG AAGAAAAGTC ATGAATCGGA CACTATGTCT 
AACAGAATTG TGCATATTAG TGTTCAGTTG TTCAGCAATG AAGAGCTAGC CAGACAGGTA 
ACAGAAGAAT GTCAGCTGCT GGATATTATG GTCACTGTGC TGTTATACAT GATGGAAAGT 
TGCCTTATTA AAAGTGAGCT ACAAGATGAA GAAAATAGTT TACACGTTGT AGTGAATTGT 
GGAGAAGCAT TGCTGAAGAA TAACACTTAT TGGCCTCTTG TCAGTGATTT TATTAATATC 
CTTTCTCATC AAAGTGTGGC TAAGAGATTT TTGGAGGACC ATGGTTTGTT AGTGACATGG 
ATGAACTTTG TATCTTTCTT TCAAGGTATG AACTTAAATA AGCGAGAACT AAATGAGCAT 
GTGGAGTTTG AATCTCAGAC CTACTATGCT GCCTTTGCTG CTGAACTTGA AGCCTGTGCA 
CAGCCAATGT GGGGACTCTT ATCACACTGT AAAGTTAGGG AAACTCAAGA ATATACCCGA 
AATGTTGTTA GATATTGCCT TGAAGCTCTT CAAGACTGGT TTGATGCTAT TAACTTCGTA 
GATGAGCCAG CACCTAACCA AGTTACTTTT CATCTGCCAC TACATCGTTA TTATGCTATG 
TTTTTGAGTA AGGCTGTGAA ATGTCAAGAA CTAGATTTGG ATTCTGTTTT ACCAGATCAG 
GAAATGTTAA TGAAACTAAT GATTCACCCA CTCCAAATTC AGGCAAGTCT TGCTGAAATC 
CATAGCAATA TGTGGGTAAG AAATGGTCTG CAGATCAAAG GACAAGCCAT GACCTATGTC 
CAGTCTCATT TCTGTAATTC CATGATTGAT CCTGACATTT ACCTGTTACA GGTTTGTGCA 
TCTAGACTTG ATCCGGATTA TTTTATTTCA TCTGTCTTTG AAAGATTTAA GGTAGTGGAT 
TTGTTGACAA TGGCTTCACA ACATCAAAAC ACAGTACTTG ATGCAGAGCA TGAGAGATCC 
ATGTTAGAAG GCGCTCTTAC ATTTCTTGTG ATTCTTTTGA GTCTTCGTTT ACATTTAGGA 
ATGTCTGATG ATGAGATTCT GAGAGCAGAG ATGGTAGCCC AGCTGTGTAT GAATGACAGA 
ACACACAGTT CATTGCTGGA CCTCATTCCA GAAAATCCAA ATCCTAAAAG TGGTATTATT 
CCTGGAAGTT ATAGCTTTGA ATCAGTTTTA TCAGCAGTAG CTGATTTTAA GGCTCCTGTT 
TTTGAACCTG GAGGTTCTAT GCAACAAGGC ATGTATACTC CTAAAGCTGA AGTCTGGGAT 
CATGAATTTG ACCCCGTCAT GGTCATTCTT CGAACAGTTT ACCGTAGAGA CGTGCAGTCT 
GCAATGGACA GATACACAGC ATTTTTAAAA CAGAGTGGAA AATTCCCTGG AAATCCTTGG 
CCACCCTATA AGAAAAGGAC ACCACTCCAT CCCAGCTATA AAGGTCTCAT GAGACTTTTG 
CACTGTAAAA CTTTACACAT TGTGCTATTC ACTCTGCTTT ACAAGATTTT GATGGATCAT 
CAAAATCTTT CAGAACATGT ACTCTGTATG GTTTTATATC TGATTGAATT AGGACTTGAA 
AATTCAGCTG AAGAGGAATC AGATGAAGAG GCATCAGCAT GTGGACCTGA ACGTTGTCAT 
GACAGTTGGT TTCCTGGTAG TAACTTAGTA TCGAATATGC GACACTTTAT AAATTATGTT 
AGAGTGAGAG TTCCAGAGAC TGCTCCAGAA GTGAAGAGAG ACTCACTTGC AAGTACCAGC 
TCTGATAGCT TGGGATCTTT ACAAAGTTCA CGTCGGCCTA AAGTTCTTCA AAGAGAGAAT 
TCTGGTACAG CTCAGGTTTT CAGCTTAGTA GCAGAGCGCA GAAAGAAGTT TCAAGAGATC 
ATCAATCGCA GTAACAGTGA AGCAAATCAA GTGGTTCGTC CTAAAACTTC AAGTAAATGG 
TCTGCACCTG GTTCAGCTCC ACAGTTAACT ACAGCCATTT TGGAAATTAA AGAAAGTATA 
TTGTCTTTGT TAATTAAACT TCACCACAAA CTCTCAGGAA AACAAAATTC CTACTATCCT 
CCTTGGCTTG ATGACATAGA AATTTTAATC CAACCAGAAA TTCCTAAATA TAGTCATGGA 
GATGGTATAA CTGCAGTAGA AAGAATTTTA CTAAAAGCTG CATTGCAAAG CAGAATGAAC 
AAACGCATCA TTGAAGAGAT ATGTAGAAAA GTGACCCCTC CTGTACCACC CAAAAAGATT 
ACTGCAGCAG AGAAGAAAAC ACTGGACAAA GAAGAAAGGC GACAAAAAGC TAGAGAAAGA 
CAGCAGAAAT TGCTTGCAGA GTTTGCATCA CGACAGAAAA GCTTCATGGA AACTGCCATG 
GATGTTGATT CTCCAGAGAG TGATATTCCT ATGGAGATCA CCACAGCAGA ACCTCAAGTT 
TCTGAGGCAG TATATGATTG TGTTATTTGT GGACAGAGTG GGCCCTCCTC TGAAGACAGA 
CCTACGGGAT TGGTTGTCCT GTTACAAGCA TCCTCAGTTT TGGGGCAGTG CCGTGACAAC 
ATTGAGCCAA AAAAGTTGCC TATCTCTGAA GAGGAGCAGA TATACCCCTG GGATACATGT 
GCAGCAGTTC ATGACGTGAG GCTCTCATTA TTACAACGTT ATTTTAAGGA TAGTTCTTGT 
CTCTTGGCAG TATCAATTGG CTGGGAAGGA GGTGTTTATG TACAAACCTG TGGCCACACA 
TTACATATCG ATTGCCATAA ATCTTACATG GAATCATTAC GGAATGACCA GGTTCTTCAG 
GGCTTCTCAG TGGACAAAGG AGAATTCACA TGTCCTCTCT GTAGGCAGTT TGCTAACAGT 
GTTCTTCCTT GTTATCCTGG AAGCAATGTT GAAAACCACC TTTGGCAGCG TCCTAGTAAC 
AAAAGCATAC AAGATCTCAT AAAGGAAGTG GAGGAGCTGC AAGGACGTCC GGGAGCTTTC 
CCAGTAAGCA TCAGTTCAGA AACCAACTTA AGTAAAGAAA TGGAATCTGT AATGAAAGAT 
ATAAAAAATA CCACTCAGAA GAAATACAGA GATTATAGCA AGACTCCAGG CTCACCAGAT 
AATGATTTTC TCTTTATGTA TTCTGTTGCT AGAACCAACT TGGAACTTGA ATTAATTCAT 
CGAGGAGGGA ATTTATGTTC AGGTGGTGCA AGTACAGCTG GTAAAAGGTC CTGTTTAAAA 
AAGGAAACTG AACTTCAGAG TGATTGCATT GCCAAGTCAT ACAGCGGAAA ATGTCAAAGC 
TTGCAGTTGA ACTCAGCTCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	UBR3
Entrez Gene ID	537932
Full Name	ubiquitin protein ligase E3 component n-recognin 3 (putative)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Bos taurus (cattle)	UBR3	XP_005202447.1
Mus musculus (house mouse)	Ubr3	NP_808451.2
Drosophila melanogaster (fruit fly)	CG42593	NP_001259318.1
	UBR3	XP_001099162.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000384	XP_310723.5
Homo sapiens (human)	UBR3	NP_742067.3
Pan troglodytes (chimpanzee)	UBR3	XP_003309461.1
Canis lupus familiaris (dog)	UBR3	XP_005640460.1
Rattus norvegicus (Norway rat)	Ubr3	XP_006234401.1
Gallus gallus (chicken)	ZNF650	XP_004942807.1
Danio rerio (zebrafish)	ubr3	XP_001919629.4
Xenopus tropicalis (tropical clawed frog)	ubr3	XP_002934668.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

UBR3 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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