ubr3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ubr3
Entrez Gene ID	553247
Full Name	ubiquitin protein ligase E3 component n-recognin 3
Synonyms	fb72f06,wu:fb72f06
General protein information	Preferred Names ubiquitin protein ligase E3 component n-recognin 3 Names E3 ubiquitin-protein ligase ubr3 E3 ubiquitin-protein ligase UBR3 znf650
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021478823.1	XP_021334498.1	E3 ubiquitin-protein ligase UBR3 isoform X1
XM_021478824.1	XP_021334499.1	E3 ubiquitin-protein ligase UBR3 isoform X3
XM_017357738.2	XP_017213227.1	E3 ubiquitin-protein ligase UBR3 isoform X2
XM_017357738.1	XP_017213227.1	E3 ubiquitin-protein ligase UBR3
XM_021478825.1	XP_021334500.1	E3 ubiquitin-protein ligase UBR3 isoform X4

Pathways from BioSystems

Homologies

Bos taurus (cattle)	UBR3	XP_005202447.1
Mus musculus (house mouse)	Ubr3	NP_808451.2
Drosophila melanogaster (fruit fly)	CG42593	NP_001259318.1
	UBR3	XP_001099162.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000384	XP_310723.5
Homo sapiens (human)	UBR3	NP_742067.3
Pan troglodytes (chimpanzee)	UBR3	XP_003309461.1
Canis lupus familiaris (dog)	UBR3	XP_005640460.1
Rattus norvegicus (Norway rat)	Ubr3	XP_006234401.1
Gallus gallus (chicken)	ZNF650	XP_004942807.1
Danio rerio (zebrafish)	ubr3	XP_001919629.4
Xenopus tropicalis (tropical clawed frog)	ubr3	XP_002934668.2

Gene Ontology

The following ubr3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ubr3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa144037	XM_021478823.1 Latest version!	Danio rerio ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa144038	XM_021478824.1 Latest version!	Danio rerio ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa144039	XM_021478825.1 Latest version!	Danio rerio ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa60284	XM_017357738.2 Latest version!	Danio rerio ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
View Old Accession Versions >>
ODa60284	XM_017357738.1	Danio rerio ubiquitin protein ligase E3 component n-recognin 3 (ubr3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ubr3 ( XM_021478823.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334498.1 Danio rerio ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X1, mRNA.

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CloneID	ODa144037
Clone ID Related Accession (Same CDS sequence)	XM_021478823.1
Accession Version	XM_021478823.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase UBR3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG CGTCTCTGCT GCGCCGGGAC AAGAAATCCA CCGCCGCCCA TCTGAAGGCA 
GACCTCAAGC GGACTGACAA CAGTTCAGGC TTGCGGCAGC TGCAGGAACT GCTGGATAGC 
GTACTGAACC CGGAGCGGGG CTCGGACCCG GAGGCGCTGG ACTGGTGTAA ATGGCTGCTG 
GCCGGAGGGG ACGGTTTCGA CGAGTTCTGC CGGACCGTCC GATCCTACGA TAACGCCACC 
CTCTGCGGGC TGGTGTGGAC TGCCAACTTC GTGGCCTACC GTTGCCGGAC TTGCGGCATT 
TCCCCGTGCA TGTCGTTATG CGCGGAGTGC TTCAATAACG GCGACCACAC GGGCCATGAT 
TTTAACATGT TCCGGAGTCA GGCCGGCGGG GCCTGTGACT GCGGGGACGG GAACGTGATG 
AGAGAGAGCG GGTTTTGTAA CAGACATCGA TTAAAAACAG GAGAGAACGT TCCATCAGTC 
CCTCGAGATC TGCTGCTCAT GTCTGAAATG GTGCTGCCGC GGTTTATCAT CACTATCATT 
CAATATCTGA GAGACGGCTA CACTGAACCA GAGTCGGCCG CTGACAGAGA TCTTCAGAAG 
GTTCTGCAGC AGTTGGATCC ACACATCTCC TTCTTAGAAG AGCTCACCAA GATGGGCGGA 
GCCATGCGCA CCGTTCTCAC CAAGATCTTA ACCGATCAGC AGACCTTTAA AGAGCTCAGC 
ATGGGTCAAG AGGATAATGT TTATGCAAAG AAGAACTATG AGAAGTATTT GTCGGCATTG 
AAGAGCTCAG GTCTGGTGTC AGTGGAGGAG AAAGGAGCCG CTGGAGGAGC TGGAGACGGG 
ACATCAGATG CTGCAGCAGG GGCAGGAGCG CTCAGTCTAC TGGGAGCCAC TGCTACTGCT 
AGTCTGGATG ACTCCAGCAA AGAGGAGGAT CAGGATGGGC TGCAGGGTGT GGGTCAGAGG 
AAAAGAGTGA AGCTCAGTAG CAGTACCAAA GATCCATCCA TAATGGACAC GCTGAAACAT 
AAATGCTTTT TAGAAGAGTT ACTGTTTTGG ACTATTAAGT ATGAATTTCC ACAGAAGATG 
GTGACGTTTT TGCTCAACAT GTTGCCAGAT CAGGATTACA AGATCACCTT CACTAAAACA 
TTCGTACAAC ACTATGCCTT CATCATGAAG ACCCTCATGA AGAGTCACGA GTCAGACACC 
ATGTCCAACC GCATCGTGCA CATCAGCGTG CAGCTCTTCA GCAACGAGGA GCTGGCCAGA 
CACGTCACAG AGGAGTGCCA GCTACTGGAC ATCATGGTCA CCGTTCTCCT CTACATGATG 
GAGAGCTGCC TTATCAAGAG CGAACTGCAA GATGAGGAGA ATAATCGTCA TGTGGTGGTG 
AACTGTGGCG AAGCTCTGCT GAAGAATAAC ACCTACTGGC CTTTAGTTAG TGATTTTATT 
AATATCCTAT CACACCAAAG TGTGGCCAAG CGCTTCCTGG AGGATCATTC TCTGCTGCTG 
CTCTGGATGA GTTTTGTCTC CTTCTTTCAA GGAATGAATC TGAATAAGCG GGAGCTGAAT 
GAGCACGTGG AGTTTGAGTC TCAGACATAT TACGCAGCGT TTGCTGCTGA ACTGGAGGCC 
TGCGCTCAGC CCATGTGGGG TCTCCTCACA CACTGCAAAG TTAAAGAGAC CCAAGACTAC 
ACTAAGACAG TGGTGCGGTA CTGTCTGGAA ACTCTTCAGA TGTGGTTTGA TGCCATTGGG 
TTTGTAGATG AGCCTTCCCT CAACCAGCTG ACGTTTCATT TACCTCTTCA CCGATACTAC 
GCCATGTTCC TCAGCAAGGG AGTGAAGTGT CAAGGTCTGG ATTTAGACAG TTTGCTTCCT 
GATCAGGAGA TGCTGATGAA GATCATGGTT CACCCCCTAC AGATTCAGGC TAGCCTGTCT 
GAGATCCATA GTAACATGTG GGTGAGAAAT GGGCTGCAGA TTAAAGGTCA GGCCATGACC 
TATGTCCAGT CTCACTTCTG CAACTCCATG ATTGATCCGG ACATCTACCT CCTGCAGGTG 
TGCGCTTCTA GACTGGATCC AGATTACTTC ATTTCCTCAG TCTTCGAGAG GTTCAAGGTG 
GTGGATCTGC TGACGATGGC GTCTCAACAT CAGAACGCAG TGCTGGACTC TGAACAAGAG 
AGGCCCATGC TGGAGGGGGC GCTCACCTTC TTGGTCATAC TGTGCAGTCT CAGGATACAC 
TTAGGAATGT CTGACGATGA GATTTTGAGA GCCGAGATGG TGTCACAGTT GTGTATGAAT 
GATCGTACCC ACAGCTCTCT GCTGGATCTT ATCCCTGAGA ACCCGAACCC TAAGAGCGGT 
GTGGTGCCGG GCAGCTGTAG TTTTGAGGAG ATGCTGTCTG GTGTAGCGGA TTTTAAAGCC 
CCTGTGTTTG AGCCTGGAGG ATCCATGCAG CAGGGCATGT ACACACCTAA AGCGGAGGTT 
TGGGAAAAAG AGTTTGACCC TATAATGGTG ATTCTACGCA CCGTTTACCG CAGAGACGTG 
CAGTCGGCCA TGGATCGATA CTCAGCATTT TTGAAACAGT CAGGCGTTCA CACAGGGAAC 
CCTTGGCCAC CCTATAAGGA GAGGACTCCT TTGCATCCCT GCTATAAAGG ACTGGTGCGC 
CTGCTGCACT GCAAAACCTT ACATATCGTC ATCTTTACAC TGCTTTATAA GATATGGATG 
GATCATCAGA ACATGTCTGA GCATGTGTTG TGTATGGTGC TGTACCTGAT AGAGCTTGGA 
CTTGATAATC AGGTCCAGGA TGACAAAGTG GAAGAGGAGC CCTGTATCGA GGAGCACTGC 
CACGACAGCT GGTTCCCGGG CACCAGCCTG CTGTCTAACC TCCATCACGT CATCAACTTT 
GTGCGGGTTC GAGTGCCGGA GACGGCTCCG GAGGTGGAGA GGAAGCGAGA GAGGGAACGA 
GAGAGAGAAA CTCCACCCAG CACCAGTTCA GAGAGCGCCA CCTTTGGTCA GAGCTCCAGA 
AGGCTTACAG GAAATTGGAG AGAGAATCTG CGTGAAGCTC AGGTGTTCAG TCTGGTGGCG 
GAGCGCAGGA GAAAGTTCCA GGAGATCATC AACCGCAGCA ACCATGAGGC CAGTCAGGCA 
GTGCGCCCCA AGTCCTCAGC GTCCCGCTGG CTTCCACCGG GAACGCCTCC ACAGCTGGTG 
ACCGAGATCC TGGAGATACG CGAGAGCATG CTGTCGCTGC TGGTTAAACT GCACCAGAAA 
CTGTCGGCCA AGCAGAACTC TCTGTCTCTG AGCTGGTTGG CCGAGGTGGA TCCTGCCCAT 
CATGCGCACG GAGACGGTTT AACGGCCATC GAAAGAATCT TAGCCAAAGC TTCAGCTCGA 
AGCAGACACA GTAAAAGATG CCTACAGGAG ATCTGCGGGA AAGTGTGTCC ACCCATACCG 
CCAAAGAAGA TCAGTCCTGG AGATAAGAAG AGCATGGACA AGGAGGAGAG GCGTCAGCGC 
GCCAGAGAGA GGCAGCAGAA GCTGCTGGCG GAGTTTGCCT CCAGGCAGAA GAGCTTCATG 
GAGACTGCCA TGGATGTTGA GTCCCCTGAA GCAGATGCTG TAATGGACGT CAGCTCTGAG 
GAATCTTTGG ACTCTGAGGT TCTCTATGAC TGTGTGATCT GTGGTCAGAG TGGACCGTCC 
ACTGAAGACA GACCCACAGG ACTGGTGGTT CTGCTGCAGG CTTCGTCAGT TTTGGGTCAC 
CGTTGTCGTA GTGACATGCC AAAGCGATTG CCCACCACAG ACGAAGAACA CATCTACCCT 
GAGGACACAT GTGGAGCCAC ACACGATGTC AGGCTGTCCC TTATGCAACG CTATTTTAAA 
GATAGTTCCT GTCTGCAGTC TGTGTCTATT GGCTGGGACG GAGGGGTGTA TGTGCAGACG 
TGTGGCCACA CTCTGCATAT AGACTGTCAC AAGTCCTACA TGGAGTCCTT ACGGAATGAT 
CAGGTTCTTC AGGGGATTTC TGTGGATAAA GGCGAGTTCA CTTGTCCGCT TTGCCGCCAG 
TTTGCAAACA GTGTTTTACC TTGTCGTCCT GGACGAGGGA TGGAAACCGG AGCCTGGCAC 
GCACCCAGTA CCAAGAGCAT GTCTACACTG GTGAAAGAAG TAGAAGACCT CCAGGAGCAG 
CTGGGCATTT TCCCAGTAAG AGCCAGCATA AAGCAAAGAG ATCCCATTCT GATCGAGTCA 
AACCTGAGTA AAGAGATGGA ATCTGTTATA AAGGACATTA AAAACACCAC ACAGAAGAAA 
TACATGGATT ATGGGAAGAA CCCTGGGTCA CCTGACAATG ACTTCCTCTT CATGTACTCC 
GTGGCCAGAA CAAATCTGGA GCTTGAGTTG GTTCATCGTG GTGGGAATCT ATGCAGTGGA 
GGGGCCAGTG CAGCTGCCAA ACGCTCCTGT CTCAATCAGC TGTTTCACGT GCTGGCCATG 
CACATGCGGC TCTACAGCAT AGACTCGGCA TACAATCCAT GGACCAGACT CACACTGAGC 
ACGCAAAGCA GAGAGAATGA GTACTGTGAT GAAGAGCGTC CTGAGGTTCC CATGCTTTTC 
CGAGACGTCC CATCCCTCCT AATCATCTTC ATCCTCACCA TGCCTCAGCC TTTACGCAAA 
GAGCACTTCA CGTGTGTGGT GAAGGTGTTG TACAGTCTGC AGTTCACACA GGCTCTGGCT 
GCGCTCTCCA TCAGGTTCAG CCGTGAGGAG AGACTCGCCT GGAGCAACAC TGGAGCTGCC 
AAGAAGAACC TGCCAAACTC TGACAAATCA TGGGAGTCCC TCCTGGGTCA CATGATCTCC 
GAGCTGACGA AGGCCAAAGA CGTGTATGAC ACCAACTCTG AGGAAACATC CGCTTTGAGC 
TCCAGTGTTT GGTCTCCTCA GTCCATTGAG TTCAGGCTCC AGCAGTTCTG TCTGCCCTTC 
CTGAGACTCT CCTGTTTACT GCAGCACCAC CTGTATGGAG ACAGCTTACC CGGCTGCCTG 
GTGGAGGAGG AGTTCTCTCT GCTGACGCGC TGTCTGGGTT TAGCTGCTTC TGTTCAGTGC 
AGTGGATCCA TGAGCAGCGC AGCGTGTCTG GAGTGGAACA TCAGTGCTTT TGACTTGATC 
AGTCAGTGGT GCTCAGAAGT CGTCGCTCTG TCCGACACGC CCTCCCAGCA GTCTGCGTCT 
CTGTTAGGTC AGGACCCGCA GTGGGCTGCT CCTCGTCTCC TGCAGCTTCC CGACAATTAC 
AACACCATCT TCCAGTACTA CCACAGGAAA TCCTGCAGCA GCTGCGGAAA GACCCCCAAA 
GACCCTGCGC TGTGCCTGGT GTGCGGCGCG TTCGTCTGCC TGAAGGGTCA TTGCTGTAAG 
CAGCAGGGCG TCTGCGAGTG TGTGCTGCAC TCCCAGCACT GCGGAGCGGC GACTGGTATC 
TTCCTCCTGA TAAACGCATC CGTCATCATT ATCATCCGAG GGCATCGCTT CTGCCTGTGG 
GGGTCTGTGT ATCTGGATGC TCACGGAGAG GAAGACCGAG ACCTACGTCG AGGAAAGCCT 
CTGTATTTGT GCGAGGAGAG ATATCGTGTT CTGGAGCAGC AGTGGGTCGC TCACACCTTC 
GACCACATCA ACAAGCGCTG GGGTCCTCAT TACAACGGAC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334498.1
CDS	75..5699
Translation	MMAASLLRRDKKSTAAHLKADLKRTDNSSGLRQLQELLDSVLNPERGSDPEALDWCKWLLAGGDGFDEFCRTVRSYDNATLCGLVWTANFVAYRCRTCGISPCMSLCAECFNNGDHTGHDFNMFRSQAGGACDCGDGNVMRESGFCNRHRLKTGENVPSVPRDLLLMSEMVLPRFIITIIQYLRDGYTEPESAADRDLQKVLQQLDPHISFLEELTKMGGAMRTVLTKILTDQQTFKELSMGQEDNVYAKKNYEKYLSALKSSGLVSVEEKGAAGGAGDGTSDAAAGAGALSLLGATATASLDDSSKEEDQDGLQGVGQRKRVKLSSSTKDPSIMDTLKHKCFLEELLFWTIKYEFPQKMVTFLLNMLPDQDYKITFTKTFVQHYAFIMKTLMKSHESDTMSNRIVHISVQLFSNEELARHVTEECQLLDIMVTVLLYMMESCLIKSELQDEENNRHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHSLLLLWMSFVSFFQGMNLNKRELNEHVEFESQTYYAAFAAELEACAQPMWGLLTHCKVKETQDYTKTVVRYCLETLQMWFDAIGFVDEPSLNQLTFHLPLHRYYAMFLSKGVKCQGLDLDSLLPDQEMLMKIMVHPLQIQASLSEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNAVLDSEQERPMLEGALTFLVILCSLRIHLGMSDDEILRAEMVSQLCMNDRTHSSLLDLIPENPNPKSGVVPGSCSFEEMLSGVADFKAPVFEPGGSMQQGMYTPKAEVWEKEFDPIMVILRTVYRRDVQSAMDRYSAFLKQSGVHTGNPWPPYKERTPLHPCYKGLVRLLHCKTLHIVIFTLLYKIWMDHQNMSEHVLCMVLYLIELGLDNQVQDDKVEEEPCIEEHCHDSWFPGTSLLSNLHHVINFVRVRVPETAPEVERKRERERERETPPSTSSESATFGQSSRRLTGNWRENLREAQVFSLVAERRRKFQEIINRSNHEASQAVRPKSSASRWLPPGTPPQLVTEILEIRESMLSLLVKLHQKLSAKQNSLSLSWLAEVDPAHHAHGDGLTAIERILAKASARSRHSKRCLQEICGKVCPPIPPKKISPGDKKSMDKEERRQRARERQQKLLAEFASRQKSFMETAMDVESPEADAVMDVSSEESLDSEVLYDCVICGQSGPSTEDRPTGLVVLLQASSVLGHRCRSDMPKRLPTTDEEHIYPEDTCGATHDVRLSLMQRYFKDSSCLQSVSIGWDGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGISVDKGEFTCPLCRQFANSVLPCRPGRGMETGAWHAPSTKSMSTLVKEVEDLQEQLGIFPVRASIKQRDPILIESNLSKEMESVIKDIKNTTQKKYMDYGKNPGSPDNDFLFMYSVARTNLELELVHRGGNLCSGGASAAAKRSCLNQLFHVLAMHMRLYSIDSAYNPWTRLTLSTQSRENEYCDEERPEVPMLFRDVPSLLIIFILTMPQPLRKEHFTCVVKVLYSLQFTQALAALSIRFSREERLAWSNTGAAKKNLPNSDKSWESLLGHMISELTKAKDVYDTNSEETSALSSSVWSPQSIEFRLQQFCLPFLRLSCLLQHHLYGDSLPGCLVEEEFSLLTRCLGLAASVQCSGSMSSAACLEWNISAFDLISQWCSEVVALSDTPSQQSASLLGQDPQWAAPRLLQLPDNYNTIFQYYHRKSCSSCGKTPKDPALCLVCGAFVCLKGHCCKQQGVCECVLHSQHCGAATGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYLCEERYRVLEQQWVAHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_021478823.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478823.1

ORF Insert Sequence:

ATGATGGCGG CGTCTCTGCT GCGCCGGGAC AAGAAATCCA CCGCCGCCCA TCTGAAGGCA 
GACCTCAAGC GGACTGACAA CAGTTCAGGC TTGCGGCAGC TGCAGGAACT GCTGGATAGC 
GTACTGAACC CGGAGCGGGG CTCGGACCCG GAGGCGCTGG ACTGGTGTAA ATGGCTGCTG 
GCCGGAGGGG ACGGTTTCGA CGAGTTCTGC CGGACCGTCC GATCCTACGA TAACGCCACC 
CTCTGCGGGC TGGTGTGGAC TGCCAACTTC GTGGCCTACC GTTGCCGGAC TTGCGGCATT 
TCCCCGTGCA TGTCGTTATG CGCGGAGTGC TTCAATAACG GCGACCACAC GGGCCATGAT 
TTTAACATGT TCCGGAGTCA GGCCGGCGGG GCCTGTGACT GCGGGGACGG GAACGTGATG 
AGAGAGAGCG GGTTTTGTAA CAGACATCGA TTAAAAACAG GAGAGAACGT TCCATCAGTC 
CCTCGAGATC TGCTGCTCAT GTCTGAAATG GTGCTGCCGC GGTTTATCAT CACTATCATT 
CAATATCTGA GAGACGGCTA CACTGAACCA GAGTCGGCCG CTGACAGAGA TCTTCAGAAG 
GTTCTGCAGC AGTTGGATCC ACACATCTCC TTCTTAGAAG AGCTCACCAA GATGGGCGGA 
GCCATGCGCA CCGTTCTCAC CAAGATCTTA ACCGATCAGC AGACCTTTAA AGAGCTCAGC 
ATGGGTCAAG AGGATAATGT TTATGCAAAG AAGAACTATG AGAAGTATTT GTCGGCATTG 
AAGAGCTCAG GTCTGGTGTC AGTGGAGGAG AAAGGAGCCG CTGGAGGAGC TGGAGACGGG 
ACATCAGATG CTGCAGCAGG GGCAGGAGCG CTCAGTCTAC TGGGAGCCAC TGCTACTGCT 
AGTCTGGATG ACTCCAGCAA AGAGGAGGAT CAGGATGGGC TGCAGGGTGT GGGTCAGAGG 
AAAAGAGTGA AGCTCAGTAG CAGTACCAAA GATCCATCCA TAATGGACAC GCTGAAACAT 
AAATGCTTTT TAGAAGAGTT ACTGTTTTGG ACTATTAAGT ATGAATTTCC ACAGAAGATG 
GTGACGTTTT TGCTCAACAT GTTGCCAGAT CAGGATTACA AGATCACCTT CACTAAAACA 
TTCGTACAAC ACTATGCCTT CATCATGAAG ACCCTCATGA AGAGTCACGA GTCAGACACC 
ATGTCCAACC GCATCGTGCA CATCAGCGTG CAGCTCTTCA GCAACGAGGA GCTGGCCAGA 
CACGTCACAG AGGAGTGCCA GCTACTGGAC ATCATGGTCA CCGTTCTCCT CTACATGATG 
GAGAGCTGCC TTATCAAGAG CGAACTGCAA GATGAGGAGA ATAATCGTCA TGTGGTGGTG 
AACTGTGGCG AAGCTCTGCT GAAGAATAAC ACCTACTGGC CTTTAGTTAG TGATTTTATT 
AATATCCTAT CACACCAAAG TGTGGCCAAG CGCTTCCTGG AGGATCATTC TCTGCTGCTG 
CTCTGGATGA GTTTTGTCTC CTTCTTTCAA GGAATGAATC TGAATAAGCG GGAGCTGAAT 
GAGCACGTGG AGTTTGAGTC TCAGACATAT TACGCAGCGT TTGCTGCTGA ACTGGAGGCC 
TGCGCTCAGC CCATGTGGGG TCTCCTCACA CACTGCAAAG TTAAAGAGAC CCAAGACTAC 
ACTAAGACAG TGGTGCGGTA CTGTCTGGAA ACTCTTCAGA TGTGGTTTGA TGCCATTGGG 
TTTGTAGATG AGCCTTCCCT CAACCAGCTG ACGTTTCATT TACCTCTTCA CCGATACTAC 
GCCATGTTCC TCAGCAAGGG AGTGAAGTGT CAAGGTCTGG ATTTAGACAG TTTGCTTCCT 
GATCAGGAGA TGCTGATGAA GATCATGGTT CACCCCCTAC AGATTCAGGC TAGCCTGTCT 
GAGATCCATA GTAACATGTG GGTGAGAAAT GGGCTGCAGA TTAAAGGTCA GGCCATGACC 
TATGTCCAGT CTCACTTCTG CAACTCCATG ATTGATCCGG ACATCTACCT CCTGCAGGTG 
TGCGCTTCTA GACTGGATCC AGATTACTTC ATTTCCTCAG TCTTCGAGAG GTTCAAGGTG 
GTGGATCTGC TGACGATGGC GTCTCAACAT CAGAACGCAG TGCTGGACTC TGAACAAGAG 
AGGCCCATGC TGGAGGGGGC GCTCACCTTC TTGGTCATAC TGTGCAGTCT CAGGATACAC 
TTAGGAATGT CTGACGATGA GATTTTGAGA GCCGAGATGG TGTCACAGTT GTGTATGAAT 
GATCGTACCC ACAGCTCTCT GCTGGATCTT ATCCCTGAGA ACCCGAACCC TAAGAGCGGT 
GTGGTGCCGG GCAGCTGTAG TTTTGAGGAG ATGCTGTCTG GTGTAGCGGA TTTTAAAGCC 
CCTGTGTTTG AGCCTGGAGG ATCCATGCAG CAGGGCATGT ACACACCTAA AGCGGAGGTT 
TGGGAAAAAG AGTTTGACCC TATAATGGTG ATTCTACGCA CCGTTTACCG CAGAGACGTG 
CAGTCGGCCA TGGATCGATA CTCAGCATTT TTGAAACAGT CAGGCGTTCA CACAGGGAAC 
CCTTGGCCAC CCTATAAGGA GAGGACTCCT TTGCATCCCT GCTATAAAGG ACTGGTGCGC 
CTGCTGCACT GCAAAACCTT ACATATCGTC ATCTTTACAC TGCTTTATAA GATATGGATG 
GATCATCAGA ACATGTCTGA GCATGTGTTG TGTATGGTGC TGTACCTGAT AGAGCTTGGA 
CTTGATAATC AGGTCCAGGA TGACAAAGTG GAAGAGGAGC CCTGTATCGA GGAGCACTGC 
CACGACAGCT GGTTCCCGGG CACCAGCCTG CTGTCTAACC TCCATCACGT CATCAACTTT 
GTGCGGGTTC GAGTGCCGGA GACGGCTCCG GAGGTGGAGA GGAAGCGAGA GAGGGAACGA 
GAGAGAGAAA CTCCACCCAG CACCAGTTCA GAGAGCGCCA CCTTTGGTCA GAGCTCCAGA 
AGGCTTACAG GAAATTGGAG AGAGAATCTG CGTGAAGCTC AGGTGTTCAG TCTGGTGGCG 
GAGCGCAGGA GAAAGTTCCA GGAGATCATC AACCGCAGCA ACCATGAGGC CAGTCAGGCA 
GTGCGCCCCA AGTCCTCAGC GTCCCGCTGG CTTCCACCGG GAACGCCTCC ACAGCTGGTG 
ACCGAGATCC TGGAGATACG CGAGAGCATG CTGTCGCTGC TGGTTAAACT GCACCAGAAA 
CTGTCGGCCA AGCAGAACTC TCTGTCTCTG AGCTGGTTGG CCGAGGTGGA TCCTGCCCAT 
CATGCGCACG GAGACGGTTT AACGGCCATC GAAAGAATCT TAGCCAAAGC TTCAGCTCGA 
AGCAGACACA GTAAAAGATG CCTACAGGAG ATCTGCGGGA AAGTGTGTCC ACCCATACCG 
CCAAAGAAGA TCAGTCCTGG AGATAAGAAG AGCATGGACA AGGAGGAGAG GCGTCAGCGC 
GCCAGAGAGA GGCAGCAGAA GCTGCTGGCG GAGTTTGCCT CCAGGCAGAA GAGCTTCATG 
GAGACTGCCA TGGATGTTGA GTCCCCTGAA GCAGATGCTG TAATGGACGT CAGCTCTGAG 
GAATCTTTGG ACTCTGAGGT TCTCTATGAC TGTGTGATCT GTGGTCAGAG TGGACCGTCC 
ACTGAAGACA GACCCACAGG ACTGGTGGTT CTGCTGCAGG CTTCGTCAGT TTTGGGTCAC 
CGTTGTCGTA GTGACATGCC AAAGCGATTG CCCACCACAG ACGAAGAACA CATCTACCCT 
GAGGACACAT GTGGAGCCAC ACACGATGTC AGGCTGTCCC TTATGCAACG CTATTTTAAA 
GATAGTTCCT GTCTGCAGTC TGTGTCTATT GGCTGGGACG GAGGGGTGTA TGTGCAGACG 
TGTGGCCACA CTCTGCATAT AGACTGTCAC AAGTCCTACA TGGAGTCCTT ACGGAATGAT 
CAGGTTCTTC AGGGGATTTC TGTGGATAAA GGCGAGTTCA CTTGTCCGCT TTGCCGCCAG 
TTTGCAAACA GTGTTTTACC TTGTCGTCCT GGACGAGGGA TGGAAACCGG AGCCTGGCAC 
GCACCCAGTA CCAAGAGCAT GTCTACACTG GTGAAAGAAG TAGAAGACCT CCAGGAGCAG 
CTGGGCATTT TCCCAGTAAG AGCCAGCATA AAGCAAAGAG ATCCCATTCT GATCGAGTCA 
AACCTGAGTA AAGAGATGGA ATCTGTTATA AAGGACATTA AAAACACCAC ACAGAAGAAA 
TACATGGATT ATGGGAAGAA CCCTGGGTCA CCTGACAATG ACTTCCTCTT CATGTACTCC 
GTGGCCAGAA CAAATCTGGA GCTTGAGTTG GTTCATCGTG GTGGGAATCT ATGCAGTGGA 
GGGGCCAGTG CAGCTGCCAA ACGCTCCTGT CTCAATCAGC TGTTTCACGT GCTGGCCATG 
CACATGCGGC TCTACAGCAT AGACTCGGCA TACAATCCAT GGACCAGACT CACACTGAGC 
ACGCAAAGCA GAGAGAATGA GTACTGTGAT GAAGAGCGTC CTGAGGTTCC CATGCTTTTC 
CGAGACGTCC CATCCCTCCT AATCATCTTC ATCCTCACCA TGCCTCAGCC TTTACGCAAA 
GAGCACTTCA CGTGTGTGGT GAAGGTGTTG TACAGTCTGC AGTTCACACA GGCTCTGGCT 
GCGCTCTCCA TCAGGTTCAG CCGTGAGGAG AGACTCGCCT GGAGCAACAC TGGAGCTGCC 
AAGAAGAACC TGCCAAACTC TGACAAATCA TGGGAGTCCC TCCTGGGTCA CATGATCTCC 
GAGCTGACGA AGGCCAAAGA CGTGTATGAC ACCAACTCTG AGGAAACATC CGCTTTGAGC 
TCCAGTGTTT GGTCTCCTCA GTCCATTGAG TTCAGGCTCC AGCAGTTCTG TCTGCCCTTC 
CTGAGACTCT CCTGTTTACT GCAGCACCAC CTGTATGGAG ACAGCTTACC CGGCTGCCTG 
GTGGAGGAGG AGTTCTCTCT GCTGACGCGC TGTCTGGGTT TAGCTGCTTC TGTTCAGTGC 
AGTGGATCCA TGAGCAGCGC AGCGTGTCTG GAGTGGAACA TCAGTGCTTT TGACTTGATC 
AGTCAGTGGT GCTCAGAAGT CGTCGCTCTG TCCGACACGC CCTCCCAGCA GTCTGCGTCT 
CTGTTAGGTC AGGACCCGCA GTGGGCTGCT CCTCGTCTCC TGCAGCTTCC CGACAATTAC 
AACACCATCT TCCAGTACTA CCACAGGAAA TCCTGCAGCA GCTGCGGAAA GACCCCCAAA 
GACCCTGCGC TGTGCCTGGT GTGCGGCGCG TTCGTCTGCC TGAAGGGTCA TTGCTGTAAG 
CAGCAGGGCG TCTGCGAGTG TGTGCTGCAC TCCCAGCACT GCGGAGCGGC GACTGGTATC 
TTCCTCCTGA TAAACGCATC CGTCATCATT ATCATCCGAG GGCATCGCTT CTGCCTGTGG 
GGGTCTGTGT ATCTGGATGC TCACGGAGAG GAAGACCGAG ACCTACGTCG AGGAAAGCCT 
CTGTATTTGT GCGAGGAGAG ATATCGTGTT CTGGAGCAGC AGTGGGTCGC TCACACCTTC 
GACCACATCA ACAAGCGCTG GGGTCCTCAT TACAACGGAC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ubr3 ( XM_021478824.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334499.1 Danio rerio ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X3, mRNA.

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CloneID	ODa144038
Clone ID Related Accession (Same CDS sequence)	XM_021478824.1
Accession Version	XM_021478824.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase UBR3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG CGTCTCTGCT GCGCCGGGAC AAGAAATCCA CCGCCGCCCA TCTGAAGGCA 
GACCTCAAGC GGACTGACAA CAGTTCAGGC TTGCGGCAGC TGCAGGAACT GCTGGATAGC 
GTACTGAACC CGGAGCGGGG CTCGGACCCG GAGGCGCTGG ACTGGTGTAA ATGGCTGCTG 
GCCGGAGGGG ACGGTTTCGA CGAGTTCTGC CGGACCGTCC GATCCTACGA TAACGCCACC 
CTCTGCGGGC TGGTGTGGAC TGCCAACTTC GTGGCCTACC GTTGCCGGAC TTGCGGCATT 
TCCCCGTGCA TGTCGTTATG CGCGGAGTGC TTCAATAACG GCGACCACAC GGGCCATGAT 
TTTAACATGT TCCGGAGTCA GGCCGGCGGG GCCTGTGACT GCGGGGACGG GAACGTGATG 
AGAGAGAGCG GGTTTTGTAA CAGACATCGA TTAAAAACAG GAGAGAACGT TCCATCAGTC 
CCTCGAGATC TGCTGCTCAT GTCTGAAATG GTGCTGCCGC GGTTTATCAT CACTATCATT 
CAATATCTGA GAGACGGCTA CACTGAACCA GAGTCGGCCG CTGACAGAGA TCTTCAGAAG 
GTTCTGCAGC AGTTGGATCC ACACATCTCC TTCTTAGAAG AGCTCACCAA GATGGGCGGA 
GCCATGCGCA CCGTTCTCAC CAAGATCTTA ACCGATCAGC AGACCTTTAA AGAGCTCAGC 
ATGGGTCAAG AGGATAATGT TTATGCAAAG AAGAACTATG AGAAGTATTT GTCGGCATTG 
AAGAGCTCAG GTCTGGTGTC AGTGGAGGAG AAAGGAGCCG CTGGAGGAGC TGGAGACGGG 
ACATCAGATG CTGCAGCAGG GGCAGGAGCG CTCAGTCTAC TGGGAGCCAC TGCTACTGCT 
AGTCTGGATG ACTCCAGCAA AGAGGAGGAT CAGGATGGGC TGCAGGGTGT GGGTCAGAGG 
AAAAGAGTGA AGCTCAGTAG CAGTACCAAA GATCCATCCA TAATGGACAC GCTGAAACAT 
AAATGCTTTT TAGAAGAGTT ACTGTTTTGG ACTATTAAGT ATGAATTTCC ACAGAAGATG 
GTGACGTTTT TGCTCAACAT GTTGCCAGAT CAGGATTACA AGATCACCTT CACTAAAACA 
TTCGTACAAC ACTATGCCTT CATCATGAAG ACCCTCATGA AGAGTCACGA GTCAGACACC 
ATGTCCAACC GCATCGTGCA CATCAGCGTG CAGCTCTTCA GCAACGAGGA GCTGGCCAGA 
CACGTCACAG AGGAGTGCCA GCTACTGGAC ATCATGGTCA CCGTTCTCCT CTACATGATG 
GAGAGCTGCC TTATCAAGAG CGAACTGCAA GATGAGGAGA ATAATCGTCA TGTGGTGGTG 
AACTGTGGCG AAGCTCTGCT GAAGAATAAC ACCTACTGGC CTTTAGTTAG TGATTTTATT 
AATATCCTAT CACACCAAAG TGTGGCCAAG CGCTTCCTGG AGGATCATTC TCTGCTGCTG 
CTCTGGATGA GTTTTGTCTC CTTCTTTCAA GGAATGAATC TGAATAAGCG GGAGCTGAAT 
GAGCACGTGG AGTTTGAGTC TCAGACATAT TACGCAGCGT TTGCTGCTGA ACTGGAGGCC 
TGCGCTCAGC CCATGTGGGG TCTCCTCACA CACTGCAAAG TTAAAGAGAC CCAAGACTAC 
ACTAAGACAG TGGTGCGGTA CTGTCTGGAA ACTCTTCAGA TGTGGTTTGA TGCCATTGGG 
TTTGTAGATG AGCCTTCCCT CAACCAGCTG ACGTTTCATT TACCTCTTCA CCGATACTAC 
GCCATGTTCC TCAGCAAGGG AGTGAAGTGT CAAGGTCTGG ATTTAGACAG TTTGCTTCCT 
GATCAGGAGA TGCTGATGAA GATCATGGTT CACCCCCTAC AGATTCAGGC TAGCCTGTCT 
GAGATCCATA GTAACATGTG GGTGAGAAAT GGGCTGCAGA TTAAAGGTCA GGCCATGACC 
TATGTCCAGT CTCACTTCTG CAACTCCATG ATTGATCCGG ACATCTACCT CCTGCAGGTG 
TGCGCTTCTA GACTGGATCC AGATTACTTC ATTTCCTCAG TCTTCGAGAG GTTCAAGGTG 
GTGGATCTGC TGACGATGGC GTCTCAACAT CAGAACGCAG TGCTGGACTC TGAACAAGAG 
AGGCCCATGC TGGAGGGGGC GCTCACCTTC TTGGTCATAC TGTGCAGTCT CAGGATACAC 
TTAGGAATGT CTGACGATGA GATTTTGAGA GCCGAGATGG TGTCACAGTT GTGTATGAAT 
GATCGTACCC ACAGCTCTCT GCTGGATCTT ATCCCTGAGA ACCCGAACCC TAAGAGCGGT 
GTGGTGCCGG GCAGCTGTAG TTTTGAGGAG ATGCTGTCTG GTGTAGCGGA TTTTAAAGCC 
CCTGTGTTTG AGCCTGGAGG ATCCATGCAG CAGGGCATGT ACACACCTAA AGCGGAGGTT 
TGGGAAAAAG AGTTTGACCC TATAATGGTG ATTCTACGCA CCGTTTACCG CAGAGACGTG 
CAGTCGGCCA TGGATCGATA CTCAGCATTT TTGAAACAGT CAGGCGTTCA CACAGGGAAC 
CCTTGGCCAC CCTATAAGGA GAGGACTCCT TTGCATCCCT GCTATAAAGG ACTGGTGCGC 
CTGCTGCACT GCAAAACCTT ACATATCGTC ATCTTTACAC TGCTTTATAA GATATGGATG 
GATCATCAGA ACATGTCTGA GCATGTGTTG TGTATGGTGC TGTACCTGAT AGAGCTTGGA 
CTTGATAATC AGGTCCAGGA TGACAAAGTG GAAGAGGAGC CCTGTATCGA GGAGCACTGC 
CACGACAGCT GGTTCCCGGG CACCAGCCTG CTGTCTAACC TCCATCACGT CATCAACTTT 
GTGCGGGTTC GAGTGCCGGA GACGGCTCCG GAGGTGGAGA GGAAGCGAGA GAGGGAACGA 
GAGAGAGAAA CTCCACCCAG CACCAGTTCA GAGAGCGCCA CCTTTGGTCA GAGCTCCAGA 
AGGCTTACAG GAAATTGGAG AGAGAATCTG CGTGAAGCTC AGGTGTTCAG TCTGGTGGCG 
GAGCGCAGGA GAAAGTTCCA GGAGATCATC AACCGCAGCA ACCATGAGGC CAGTCAGGCA 
GTGCGCCCCA AGTCCTCAGC GTCCCGCTGG CTTCCACCGG GAACGCCTCC ACAGCTGGTG 
ACCGAGATCC TGGAGATACG CGAGAGCATG CTGTCGCTGC TGGTTAAACT GCACCAGAAA 
CTGTCGGCCA AGCAGAACTC TCTGTCTCTG AGCTGGTTGG CCGAGGTGGA TCCTGCCCAT 
CATGCGCACG GAGACGGTTT AACGGCCATC GAAAGAATCT TAGCCAAAGC TTCAGCTCGA 
AGCAGACACA GTAAAAGATG CCTACAGGAG ATCTGCGGGA AAGTGTGTCC ACCCATACCG 
CCAAAGAAGA TCAGTCCTGG AGATAAGAAG AGCATGGACA AGGAGGAGAG GCGTCAGCGC 
GCCAGAGAGA GGCAGCAGAA GCTGCTGGCG GAGTTTGCCT CCAGGCAGAA GAGCTTCATG 
GAGACTGCCA TGGATGTTGA GTCCCCTGAA GCAGATGCTG TAATGGACGT CAGCTCTGAG 
GAATCTTTGG ACTCTGAGGT TCTCTATGAC TGTGTGATCT GTGGTCAGAG TGGACCGTCC 
ACTGAAGACA GACCCACAGG ACTGGTGGTT CTGCTGCAGG CTTCGTCAGT TTTGGGTCAC 
CGTTGTCGTA GTGACATGCC AAAGCGATTG CCCACCACAG ACGAAGAACA CATCTACCCT 
GAGGACACAT GTGGAGCCAC ACACGATGTC AGGCTGTCCC TTATGCAACG CTATTTTAAA 
GATAGTTCCT GTCTGCAGTC TGTGTCTATT GGCTGGGACG GAGGGGTGTA TGTGCAGACG 
TGTGGCCACA CTCTGCATAT AGACTGTCAC AAGTCCTACA TGGAGTCCTT ACGGAATGAT 
CAGGTTCTTC AGGGGATTTC TGTGGATAAA GGCGAGTTCA CTTGTCCGCT TTGCCGCCAG 
TTTGCAAACA GTGTTTTACC TTGTCGTCCT GGACGAGGGA TGGAAACCGG AGCCTGGCAC 
GCACCCAGTA CCAAGAGCAT GTCTACACTG GTGAAAGAAG TAGAAGACCT CCAGGAGCAG 
CTGGGCATTT TCCCAATCGA GTCAAACCTG AGTAAAGAGA TGGAATCTGT TATAAAGGAC 
ATTAAAAACA CCACACAGAA GAAATACATG GATTATGGGA AGAACCCTGG GTCACCTGAC 
AATGACTTCC TCTTCATGTA CTCCGTGGCC AGAACAAATC TGGAGCTTGA GTTGGTTCAT 
CGTGGTGGGA ATCTATGCAG TGGAGGGGCC AGTGCAGCTG CCAAACGCTC CTGTCTCAAT 
CAGCTGTTTC ACGTGCTGGC CATGCACATG CGGCTCTACA GCATAGACTC GGCATACAAT 
CCATGGACCA GACTCACACT GAGCACGCAA AGCAGAGAGA ATGAGTACTG TGATGAAGAG 
CGTCCTGAGG TTCCCATGCT TTTCCGAGAC GTCCCATCCC TCCTAATCAT CTTCATCCTC 
ACCATGCCTC AGCCTTTACG CAAAGAGCAC TTCACGTGTG TGGTGAAGGT GTTGTACAGT 
CTGCAGTTCA CACAGGCTCT GGCTGCGCTC TCCATCAGGT TCAGCCGTGA GGAGAGACTC 
GCCTGGAGCA ACACTGGAGC TGCCAAGAAG AACCTGCCAA ACTCTGACAA ATCATGGGAG 
TCCCTCCTGG GTCACATGAT CTCCGAGCTG ACGAAGGCCA AAGACGTGTA TGACACCAAC 
TCTGAGGAAA CATCCGCTTT GAGCTCCAGT GTTTGGTCTC CTCAGTCCAT TGAGTTCAGG 
CTCCAGCAGT TCTGTCTGCC CTTCCTGAGA CTCTCCTGTT TACTGCAGCA CCACCTGTAT 
GGAGACAGCT TACCCGGCTG CCTGGTGGAG GAGGAGTTCT CTCTGCTGAC GCGCTGTCTG 
GGTTTAGCTG CTTCTGTTCA GTGCAGTGGA TCCATGAGCA GCGCAGCGTG TCTGGAGTGG 
AACATCAGTG CTTTTGACTT GATCAGTCAG TGGTGCTCAG AAGTCGTCGC TCTGTCCGAC 
ACGCCCTCCC AGCAGTCTGC GTCTCTGTTA GGTCAGGACC CGCAGTGGGC TGCTCCTCGT 
CTCCTGCAGC TTCCCGACAA TTACAACACC ATCTTCCAGT ACTACCACAG GAAATCCTGC 
AGCAGCTGCG GAAAGACCCC CAAAGACCCT GCGCTGTGCC TGGTGTGCGG CGCGTTCGTC 
TGCCTGAAGG GTCATTGCTG TAAGCAGCAG GGCGTCTGCG AGTGTGTGCT GCACTCCCAG 
CACTGCGGAG CGGCGACTGG TATCTTCCTC CTGATAAACG CATCCGTCAT CATTATCATC 
CGAGGGCATC GCTTCTGCCT GTGGGGGTCT GTGTATCTGG ATGCTCACGG AGAGGAAGAC 
CGAGACCTAC GTCGAGGAAA GCCTCTGTAT TTGTGCGAGG AGAGATATCG TGTTCTGGAG 
CAGCAGTGGG TCGCTCACAC CTTCGACCAC ATCAACAAGC GCTGGGGTCC TCATTACAAC 
GGACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334499.1
CDS	74..5662
Translation	MMAASLLRRDKKSTAAHLKADLKRTDNSSGLRQLQELLDSVLNPERGSDPEALDWCKWLLAGGDGFDEFCRTVRSYDNATLCGLVWTANFVAYRCRTCGISPCMSLCAECFNNGDHTGHDFNMFRSQAGGACDCGDGNVMRESGFCNRHRLKTGENVPSVPRDLLLMSEMVLPRFIITIIQYLRDGYTEPESAADRDLQKVLQQLDPHISFLEELTKMGGAMRTVLTKILTDQQTFKELSMGQEDNVYAKKNYEKYLSALKSSGLVSVEEKGAAGGAGDGTSDAAAGAGALSLLGATATASLDDSSKEEDQDGLQGVGQRKRVKLSSSTKDPSIMDTLKHKCFLEELLFWTIKYEFPQKMVTFLLNMLPDQDYKITFTKTFVQHYAFIMKTLMKSHESDTMSNRIVHISVQLFSNEELARHVTEECQLLDIMVTVLLYMMESCLIKSELQDEENNRHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHSLLLLWMSFVSFFQGMNLNKRELNEHVEFESQTYYAAFAAELEACAQPMWGLLTHCKVKETQDYTKTVVRYCLETLQMWFDAIGFVDEPSLNQLTFHLPLHRYYAMFLSKGVKCQGLDLDSLLPDQEMLMKIMVHPLQIQASLSEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNAVLDSEQERPMLEGALTFLVILCSLRIHLGMSDDEILRAEMVSQLCMNDRTHSSLLDLIPENPNPKSGVVPGSCSFEEMLSGVADFKAPVFEPGGSMQQGMYTPKAEVWEKEFDPIMVILRTVYRRDVQSAMDRYSAFLKQSGVHTGNPWPPYKERTPLHPCYKGLVRLLHCKTLHIVIFTLLYKIWMDHQNMSEHVLCMVLYLIELGLDNQVQDDKVEEEPCIEEHCHDSWFPGTSLLSNLHHVINFVRVRVPETAPEVERKRERERERETPPSTSSESATFGQSSRRLTGNWRENLREAQVFSLVAERRRKFQEIINRSNHEASQAVRPKSSASRWLPPGTPPQLVTEILEIRESMLSLLVKLHQKLSAKQNSLSLSWLAEVDPAHHAHGDGLTAIERILAKASARSRHSKRCLQEICGKVCPPIPPKKISPGDKKSMDKEERRQRARERQQKLLAEFASRQKSFMETAMDVESPEADAVMDVSSEESLDSEVLYDCVICGQSGPSTEDRPTGLVVLLQASSVLGHRCRSDMPKRLPTTDEEHIYPEDTCGATHDVRLSLMQRYFKDSSCLQSVSIGWDGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGISVDKGEFTCPLCRQFANSVLPCRPGRGMETGAWHAPSTKSMSTLVKEVEDLQEQLGIFPIESNLSKEMESVIKDIKNTTQKKYMDYGKNPGSPDNDFLFMYSVARTNLELELVHRGGNLCSGGASAAAKRSCLNQLFHVLAMHMRLYSIDSAYNPWTRLTLSTQSRENEYCDEERPEVPMLFRDVPSLLIIFILTMPQPLRKEHFTCVVKVLYSLQFTQALAALSIRFSREERLAWSNTGAAKKNLPNSDKSWESLLGHMISELTKAKDVYDTNSEETSALSSSVWSPQSIEFRLQQFCLPFLRLSCLLQHHLYGDSLPGCLVEEEFSLLTRCLGLAASVQCSGSMSSAACLEWNISAFDLISQWCSEVVALSDTPSQQSASLLGQDPQWAAPRLLQLPDNYNTIFQYYHRKSCSSCGKTPKDPALCLVCGAFVCLKGHCCKQQGVCECVLHSQHCGAATGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYLCEERYRVLEQQWVAHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_021478824.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478824.1

ORF Insert Sequence:

ATGATGGCGG CGTCTCTGCT GCGCCGGGAC AAGAAATCCA CCGCCGCCCA TCTGAAGGCA 
GACCTCAAGC GGACTGACAA CAGTTCAGGC TTGCGGCAGC TGCAGGAACT GCTGGATAGC 
GTACTGAACC CGGAGCGGGG CTCGGACCCG GAGGCGCTGG ACTGGTGTAA ATGGCTGCTG 
GCCGGAGGGG ACGGTTTCGA CGAGTTCTGC CGGACCGTCC GATCCTACGA TAACGCCACC 
CTCTGCGGGC TGGTGTGGAC TGCCAACTTC GTGGCCTACC GTTGCCGGAC TTGCGGCATT 
TCCCCGTGCA TGTCGTTATG CGCGGAGTGC TTCAATAACG GCGACCACAC GGGCCATGAT 
TTTAACATGT TCCGGAGTCA GGCCGGCGGG GCCTGTGACT GCGGGGACGG GAACGTGATG 
AGAGAGAGCG GGTTTTGTAA CAGACATCGA TTAAAAACAG GAGAGAACGT TCCATCAGTC 
CCTCGAGATC TGCTGCTCAT GTCTGAAATG GTGCTGCCGC GGTTTATCAT CACTATCATT 
CAATATCTGA GAGACGGCTA CACTGAACCA GAGTCGGCCG CTGACAGAGA TCTTCAGAAG 
GTTCTGCAGC AGTTGGATCC ACACATCTCC TTCTTAGAAG AGCTCACCAA GATGGGCGGA 
GCCATGCGCA CCGTTCTCAC CAAGATCTTA ACCGATCAGC AGACCTTTAA AGAGCTCAGC 
ATGGGTCAAG AGGATAATGT TTATGCAAAG AAGAACTATG AGAAGTATTT GTCGGCATTG 
AAGAGCTCAG GTCTGGTGTC AGTGGAGGAG AAAGGAGCCG CTGGAGGAGC TGGAGACGGG 
ACATCAGATG CTGCAGCAGG GGCAGGAGCG CTCAGTCTAC TGGGAGCCAC TGCTACTGCT 
AGTCTGGATG ACTCCAGCAA AGAGGAGGAT CAGGATGGGC TGCAGGGTGT GGGTCAGAGG 
AAAAGAGTGA AGCTCAGTAG CAGTACCAAA GATCCATCCA TAATGGACAC GCTGAAACAT 
AAATGCTTTT TAGAAGAGTT ACTGTTTTGG ACTATTAAGT ATGAATTTCC ACAGAAGATG 
GTGACGTTTT TGCTCAACAT GTTGCCAGAT CAGGATTACA AGATCACCTT CACTAAAACA 
TTCGTACAAC ACTATGCCTT CATCATGAAG ACCCTCATGA AGAGTCACGA GTCAGACACC 
ATGTCCAACC GCATCGTGCA CATCAGCGTG CAGCTCTTCA GCAACGAGGA GCTGGCCAGA 
CACGTCACAG AGGAGTGCCA GCTACTGGAC ATCATGGTCA CCGTTCTCCT CTACATGATG 
GAGAGCTGCC TTATCAAGAG CGAACTGCAA GATGAGGAGA ATAATCGTCA TGTGGTGGTG 
AACTGTGGCG AAGCTCTGCT GAAGAATAAC ACCTACTGGC CTTTAGTTAG TGATTTTATT 
AATATCCTAT CACACCAAAG TGTGGCCAAG CGCTTCCTGG AGGATCATTC TCTGCTGCTG 
CTCTGGATGA GTTTTGTCTC CTTCTTTCAA GGAATGAATC TGAATAAGCG GGAGCTGAAT 
GAGCACGTGG AGTTTGAGTC TCAGACATAT TACGCAGCGT TTGCTGCTGA ACTGGAGGCC 
TGCGCTCAGC CCATGTGGGG TCTCCTCACA CACTGCAAAG TTAAAGAGAC CCAAGACTAC 
ACTAAGACAG TGGTGCGGTA CTGTCTGGAA ACTCTTCAGA TGTGGTTTGA TGCCATTGGG 
TTTGTAGATG AGCCTTCCCT CAACCAGCTG ACGTTTCATT TACCTCTTCA CCGATACTAC 
GCCATGTTCC TCAGCAAGGG AGTGAAGTGT CAAGGTCTGG ATTTAGACAG TTTGCTTCCT 
GATCAGGAGA TGCTGATGAA GATCATGGTT CACCCCCTAC AGATTCAGGC TAGCCTGTCT 
GAGATCCATA GTAACATGTG GGTGAGAAAT GGGCTGCAGA TTAAAGGTCA GGCCATGACC 
TATGTCCAGT CTCACTTCTG CAACTCCATG ATTGATCCGG ACATCTACCT CCTGCAGGTG 
TGCGCTTCTA GACTGGATCC AGATTACTTC ATTTCCTCAG TCTTCGAGAG GTTCAAGGTG 
GTGGATCTGC TGACGATGGC GTCTCAACAT CAGAACGCAG TGCTGGACTC TGAACAAGAG 
AGGCCCATGC TGGAGGGGGC GCTCACCTTC TTGGTCATAC TGTGCAGTCT CAGGATACAC 
TTAGGAATGT CTGACGATGA GATTTTGAGA GCCGAGATGG TGTCACAGTT GTGTATGAAT 
GATCGTACCC ACAGCTCTCT GCTGGATCTT ATCCCTGAGA ACCCGAACCC TAAGAGCGGT 
GTGGTGCCGG GCAGCTGTAG TTTTGAGGAG ATGCTGTCTG GTGTAGCGGA TTTTAAAGCC 
CCTGTGTTTG AGCCTGGAGG ATCCATGCAG CAGGGCATGT ACACACCTAA AGCGGAGGTT 
TGGGAAAAAG AGTTTGACCC TATAATGGTG ATTCTACGCA CCGTTTACCG CAGAGACGTG 
CAGTCGGCCA TGGATCGATA CTCAGCATTT TTGAAACAGT CAGGCGTTCA CACAGGGAAC 
CCTTGGCCAC CCTATAAGGA GAGGACTCCT TTGCATCCCT GCTATAAAGG ACTGGTGCGC 
CTGCTGCACT GCAAAACCTT ACATATCGTC ATCTTTACAC TGCTTTATAA GATATGGATG 
GATCATCAGA ACATGTCTGA GCATGTGTTG TGTATGGTGC TGTACCTGAT AGAGCTTGGA 
CTTGATAATC AGGTCCAGGA TGACAAAGTG GAAGAGGAGC CCTGTATCGA GGAGCACTGC 
CACGACAGCT GGTTCCCGGG CACCAGCCTG CTGTCTAACC TCCATCACGT CATCAACTTT 
GTGCGGGTTC GAGTGCCGGA GACGGCTCCG GAGGTGGAGA GGAAGCGAGA GAGGGAACGA 
GAGAGAGAAA CTCCACCCAG CACCAGTTCA GAGAGCGCCA CCTTTGGTCA GAGCTCCAGA 
AGGCTTACAG GAAATTGGAG AGAGAATCTG CGTGAAGCTC AGGTGTTCAG TCTGGTGGCG 
GAGCGCAGGA GAAAGTTCCA GGAGATCATC AACCGCAGCA ACCATGAGGC CAGTCAGGCA 
GTGCGCCCCA AGTCCTCAGC GTCCCGCTGG CTTCCACCGG GAACGCCTCC ACAGCTGGTG 
ACCGAGATCC TGGAGATACG CGAGAGCATG CTGTCGCTGC TGGTTAAACT GCACCAGAAA 
CTGTCGGCCA AGCAGAACTC TCTGTCTCTG AGCTGGTTGG CCGAGGTGGA TCCTGCCCAT 
CATGCGCACG GAGACGGTTT AACGGCCATC GAAAGAATCT TAGCCAAAGC TTCAGCTCGA 
AGCAGACACA GTAAAAGATG CCTACAGGAG ATCTGCGGGA AAGTGTGTCC ACCCATACCG 
CCAAAGAAGA TCAGTCCTGG AGATAAGAAG AGCATGGACA AGGAGGAGAG GCGTCAGCGC 
GCCAGAGAGA GGCAGCAGAA GCTGCTGGCG GAGTTTGCCT CCAGGCAGAA GAGCTTCATG 
GAGACTGCCA TGGATGTTGA GTCCCCTGAA GCAGATGCTG TAATGGACGT CAGCTCTGAG 
GAATCTTTGG ACTCTGAGGT TCTCTATGAC TGTGTGATCT GTGGTCAGAG TGGACCGTCC 
ACTGAAGACA GACCCACAGG ACTGGTGGTT CTGCTGCAGG CTTCGTCAGT TTTGGGTCAC 
CGTTGTCGTA GTGACATGCC AAAGCGATTG CCCACCACAG ACGAAGAACA CATCTACCCT 
GAGGACACAT GTGGAGCCAC ACACGATGTC AGGCTGTCCC TTATGCAACG CTATTTTAAA 
GATAGTTCCT GTCTGCAGTC TGTGTCTATT GGCTGGGACG GAGGGGTGTA TGTGCAGACG 
TGTGGCCACA CTCTGCATAT AGACTGTCAC AAGTCCTACA TGGAGTCCTT ACGGAATGAT 
CAGGTTCTTC AGGGGATTTC TGTGGATAAA GGCGAGTTCA CTTGTCCGCT TTGCCGCCAG 
TTTGCAAACA GTGTTTTACC TTGTCGTCCT GGACGAGGGA TGGAAACCGG AGCCTGGCAC 
GCACCCAGTA CCAAGAGCAT GTCTACACTG GTGAAAGAAG TAGAAGACCT CCAGGAGCAG 
CTGGGCATTT TCCCAATCGA GTCAAACCTG AGTAAAGAGA TGGAATCTGT TATAAAGGAC 
ATTAAAAACA CCACACAGAA GAAATACATG GATTATGGGA AGAACCCTGG GTCACCTGAC 
AATGACTTCC TCTTCATGTA CTCCGTGGCC AGAACAAATC TGGAGCTTGA GTTGGTTCAT 
CGTGGTGGGA ATCTATGCAG TGGAGGGGCC AGTGCAGCTG CCAAACGCTC CTGTCTCAAT 
CAGCTGTTTC ACGTGCTGGC CATGCACATG CGGCTCTACA GCATAGACTC GGCATACAAT 
CCATGGACCA GACTCACACT GAGCACGCAA AGCAGAGAGA ATGAGTACTG TGATGAAGAG 
CGTCCTGAGG TTCCCATGCT TTTCCGAGAC GTCCCATCCC TCCTAATCAT CTTCATCCTC 
ACCATGCCTC AGCCTTTACG CAAAGAGCAC TTCACGTGTG TGGTGAAGGT GTTGTACAGT 
CTGCAGTTCA CACAGGCTCT GGCTGCGCTC TCCATCAGGT TCAGCCGTGA GGAGAGACTC 
GCCTGGAGCA ACACTGGAGC TGCCAAGAAG AACCTGCCAA ACTCTGACAA ATCATGGGAG 
TCCCTCCTGG GTCACATGAT CTCCGAGCTG ACGAAGGCCA AAGACGTGTA TGACACCAAC 
TCTGAGGAAA CATCCGCTTT GAGCTCCAGT GTTTGGTCTC CTCAGTCCAT TGAGTTCAGG 
CTCCAGCAGT TCTGTCTGCC CTTCCTGAGA CTCTCCTGTT TACTGCAGCA CCACCTGTAT 
GGAGACAGCT TACCCGGCTG CCTGGTGGAG GAGGAGTTCT CTCTGCTGAC GCGCTGTCTG 
GGTTTAGCTG CTTCTGTTCA GTGCAGTGGA TCCATGAGCA GCGCAGCGTG TCTGGAGTGG 
AACATCAGTG CTTTTGACTT GATCAGTCAG TGGTGCTCAG AAGTCGTCGC TCTGTCCGAC 
ACGCCCTCCC AGCAGTCTGC GTCTCTGTTA GGTCAGGACC CGCAGTGGGC TGCTCCTCGT 
CTCCTGCAGC TTCCCGACAA TTACAACACC ATCTTCCAGT ACTACCACAG GAAATCCTGC 
AGCAGCTGCG GAAAGACCCC CAAAGACCCT GCGCTGTGCC TGGTGTGCGG CGCGTTCGTC 
TGCCTGAAGG GTCATTGCTG TAAGCAGCAG GGCGTCTGCG AGTGTGTGCT GCACTCCCAG 
CACTGCGGAG CGGCGACTGG TATCTTCCTC CTGATAAACG CATCCGTCAT CATTATCATC 
CGAGGGCATC GCTTCTGCCT GTGGGGGTCT GTGTATCTGG ATGCTCACGG AGAGGAAGAC 
CGAGACCTAC GTCGAGGAAA GCCTCTGTAT TTGTGCGAGG AGAGATATCG TGTTCTGGAG 
CAGCAGTGGG TCGCTCACAC CTTCGACCAC ATCAACAAGC GCTGGGGTCC TCATTACAAC 
GGACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ubr3 ( XM_021478825.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334500.1 Danio rerio ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa144039
Clone ID Related Accession (Same CDS sequence)	XM_021478825.1
Accession Version	XM_021478825.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase UBR3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG CGTCTCTGCT GCGCCGGGAC AAGAAATCCA CCGCCGCCCA TCTGAAGGCA 
GACCTCAAGC GGACTGACAA CAGTTCAGGC TTGCGGCAGC TGCAGGAACT GCTGGATAGC 
GTACTGAACC CGGAGCGGGG CTCGGACCCG GAGGCGCTGG ACTGGTGTAA ATGGCTGCTG 
GCCGGAGGGG ACGGTTTCGA CGAGTTCTGC CGGACCGTCC GATCCTACGA TAACGCCACC 
CTCTGCGGGC TGGTGTGGAC TGCCAACTTC GTGGCCTACC GTTGCCGGAC TTGCGGCATT 
TCCCCGTGCA TGTCGTTATG CGCGGAGTGC TTCAATAACG GCGACCACAC GGGCCATGAT 
TTTAACATGT TCCGGAGTCA GGCCGGCGGG GCCTGTGACT GCGGGGACGG GAACGTGATG 
AGAGAGAGCG GGTTTTGTAA CAGACATCGA TTAAAAACAG GAGAGAACGT TCCATCAGTC 
CCTCGAGATC TGCTGCTCAT GTCTGAAATG GTGCTGCCGC GGTTTATCAT CACTATCATT 
CAATATCTGA GAGACGGCTA CACTGAACCA GAGTCGGCCG CTGACAGAGA TCTTCAGAAG 
GTTCTGCAGC AGTTGGATCC ACACATCTCC TTCTTAGAAG AGCTCACCAA GATGGGCGGA 
GCCATGCGCA CCGTTCTCAC CAAGATCTTA ACCGATCAGC AGACCTTTAA AGAGCTCAGC 
ATGGGTCAAG AGGATAATGT TTATGCAAAG AAGAACTATG AGAAGTATTT GTCGGCATTG 
AAGAGCTCAG GTCTGGTGTC AGTGGAGGAG AAAGGAGCCG CTGGAGGAGC TGGAGACGGG 
ACATCAGATG CTGCAGCAGG GGCAGGAGCG CTCAGTCTAC TGGGAGCCAC TGCTACTGCT 
AGTCTGGATG ACTCCAGCAA AGAGGAGGAT CAGGATGGGC TGCAGGGTGT GGGTCAGAGG 
AAAAGAGTGA AGCTCAGTAG CAGTACCAAA GATCCATCCA TAATGGACAC GCTGAAACAT 
AAATGCTTTT TAGAAGAGTT ACTGTTTTGG ACTATTAAGT ATGAATTTCC ACAGAAGATG 
GTGACGTTTT TGCTCAACAT GTTGCCAGAT CAGGATTACA AGATCACCTT CACTAAAACA 
TTCGTACAAC ACTATGCCTT CATCATGAAG ACCCTCATGA AGAGTCACGA GTCAGACACC 
ATGTCCAACC GCATCGTGCA CATCAGCGTG CAGCTCTTCA GCAACGAGGA GCTGGCCAGA 
CACGTCACAG AGGAGTGCCA GCTACTGGAC ATCATGGTCA CCGTTCTCCT CTACATGATG 
GAGAGCTGCC TTATCAAGAG CGAACTGCAA GATGAGGAGA ATAATCGTCA TGTGGTGGTG 
AACTGTGGCG AAGCTCTGCT GAAGAATAAC ACCTACTGGC CTTTAGTTAG TGATTTTATT 
AATATCCTAT CACACCAAAG TGTGGCCAAG CGCTTCCTGG AGGATCATTC TCTGCTGCTG 
CTCTGGATGA GTTTTGTCTC CTTCTTTCAA GGAATGAATC TGAATAAGCG GGAGCTGAAT 
GAGCACGTGG AGTTTGAGTC TCAGACATAT TACGCAGCGT TTGCTGCTGA ACTGGAGGCC 
TGCGCTCAGC CCATGTGGGG TCTCCTCACA CACTGCAAAG TTAAAGAGAC CCAAGACTAC 
ACTAAGACAG TGGTGCGGTA CTGTCTGGAA ACTCTTCAGA TGTGGTTTGA TGCCATTGGG 
TTTGTAGATG AGCCTTCCCT CAACCAGCTG ACGTTTCATT TACCTCTTCA CCGATACTAC 
GCCATGTTCC TCAGCAAGGG AGTGAAGTGT CAAGGTCTGG ATTTAGACAG TTTGCTTCCT 
GATCAGGAGA TGCTGATGAA GATCATGGTT CACCCCCTAC AGATTCAGGC TAGCCTGTCT 
GAGATCCATA GTAACATGTG GGTGAGAAAT GGGCTGCAGA TTAAAGGTCA GGCCATGACC 
TATGTCCAGT CTCACTTCTG CAACTCCATG ATTGATCCGG ACATCTACCT CCTGCAGGTG 
TGCGCTTCTA GACTGGATCC AGATTACTTC ATTTCCTCAG TCTTCGAGAG GTTCAAGGTG 
GTGGATCTGC TGACGATGGC GTCTCAACAT CAGAACGCAG TGCTGGACTC TGAACAAGAG 
AGGCCCATGC TGGAGGGGGC GCTCACCTTC TTGGTCATAC TGTGCAGTCT CAGGATACAC 
TTAGGAATGT CTGACGATGA GATTTTGAGA GCCGAGATGG TGTCACAGTT GTGTATGAAT 
GATCGTACCC ACAGCTCTCT GCTGGATCTT ATCCCTGAGA ACCCGAACCC TAAGAGCGGT 
GTGGTGCCGG GCAGCTGTAG TTTTGAGGAG ATGCTGTCTG GTGTAGCGGA TTTTAAAGCC 
CCTGTGTTTG AGCCTGGAGG ATCCATGCAG CAGGGCATGT ACACACCTAA AGCGGAGGTT 
TGGGAAAAAG AGTTTGACCC TATAATGGTG ATTCTACGCA CCGTTTACCG CAGAGACGTG 
CAGTCGGCCA TGGATCGATA CTCAGCATTT TTGAAACAGT CAGGCGTTCA CACAGGGAAC 
CCTTGGCCAC CCTATAAGGA GAGGACTCCT TTGCATCCCT GCTATAAAGG ACTGGTGCGC 
CTGCTGCACT GCAAAACCTT ACATATCGTC ATCTTTACAC TGCTTTATAA GATATGGATG 
GATCATCAGA ACATGTCTGA GCATGTGTTG TGTATGGTGC TGTACCTGAT AGAGCTTGGA 
CTTGATAATC AGGTCCAGGA TGACAAAGTG GAAGAGGAGC CCTGTATCGA GGAGCACTGC 
CACGACAGCT GGTTCCCGGG CACCAGCCTG CTGTCTAACC TCCATCACGT CATCAACTTT 
GTGCGGGTTC GAGTGCCGGA GACGGCTCCG GAGGTGGAGA GGAAGCGAGA GAGGGAACGA 
GAGAGAGAAA CTCCACCCAG CACCAGTTCA GAGAGCGCCA CCTTTGGTCA GAATCTGCGT 
GAAGCTCAGG TGTTCAGTCT GGTGGCGGAG CGCAGGAGAA AGTTCCAGGA GATCATCAAC 
CGCAGCAACC ATGAGGCCAG TCAGGCAGTG CGCCCCAAGT CCTCAGCGTC CCGCTGGCTT 
CCACCGGGAA CGCCTCCACA GCTGGTGACC GAGATCCTGG AGATACGCGA GAGCATGCTG 
TCGCTGCTGG TTAAACTGCA CCAGAAACTG TCGGCCAAGC AGAACTCTCT GTCTCTGAGC 
TGGTTGGCCG AGGTGGATCC TGCCCATCAT GCGCACGGAG ACGGTTTAAC GGCCATCGAA 
AGAATCTTAG CCAAAGCTTC AGCTCGAAGC AGACACAGTA AAAGATGCCT ACAGGAGATC 
TGCGGGAAAG TGTGTCCACC CATACCGCCA AAGAAGATCA GTCCTGGAGA TAAGAAGAGC 
ATGGACAAGG AGGAGAGGCG TCAGCGCGCC AGAGAGAGGC AGCAGAAGCT GCTGGCGGAG 
TTTGCCTCCA GGCAGAAGAG CTTCATGGAG ACTGCCATGG ATGTTGAGTC CCCTGAAGCA 
GATGCTGTAA TGGACGTCAG CTCTGAGGAA TCTTTGGACT CTGAGGTTCT CTATGACTGT 
GTGATCTGTG GTCAGAGTGG ACCGTCCACT GAAGACAGAC CCACAGGACT GGTGGTTCTG 
CTGCAGGCTT CGTCAGTTTT GGGTCACCGT TGTCGTAGTG ACATGCCAAA GCGATTGCCC 
ACCACAGACG AAGAACACAT CTACCCTGAG GACACATGTG GAGCCACACA CGATGTCAGG 
CTGTCCCTTA TGCAACGCTA TTTTAAAGAT AGTTCCTGTC TGCAGTCTGT GTCTATTGGC 
TGGGACGGAG GGGTGTATGT GCAGACGTGT GGCCACACTC TGCATATAGA CTGTCACAAG 
TCCTACATGG AGTCCTTACG GAATGATCAG GTTCTTCAGG GGATTTCTGT GGATAAAGGC 
GAGTTCACTT GTCCGCTTTG CCGCCAGTTT GCAAACAGTG TTTTACCTTG TCGTCCTGGA 
CGAGGGATGG AAACCGGAGC CTGGCACGCA CCCAGTACCA AGAGCATGTC TACACTGGTG 
AAAGAAGTAG AAGACCTCCA GGAGCAGCTG GGCATTTTCC CAATCGAGTC AAACCTGAGT 
AAAGAGATGG AATCTGTTAT AAAGGACATT AAAAACACCA CACAGAAGAA ATACATGGAT 
TATGGGAAGA ACCCTGGGTC ACCTGACAAT GACTTCCTCT TCATGTACTC CGTGGCCAGA 
ACAAATCTGG AGCTTGAGTT GGTTCATCGT GGTGGGAATC TATGCAGTGG AGGGGCCAGT 
GCAGCTGCCA AACGCTCCTG TCTCAATCAG CTGTTTCACG TGCTGGCCAT GCACATGCGG 
CTCTACAGCA TAGACTCGGC ATACAATCCA TGGACCAGAC TCACACTGAG CACGCAAAGC 
AGAGAGAATG AGTACTGTGA TGAAGAGCGT CCTGAGGTTC CCATGCTTTT CCGAGACGTC 
CCATCCCTCC TAATCATCTT CATCCTCACC ATGCCTCAGC CTTTACGCAA AGAGCACTTC 
ACGTGTGTGG TGAAGGTGTT GTACAGTCTG CAGTTCACAC AGGCTCTGGC TGCGCTCTCC 
ATCAGGTTCA GCCGTGAGGA GAGACTCGCC TGGAGCAACA CTGGAGCTGC CAAGAAGAAC 
CTGCCAAACT CTGACAAATC ATGGGAGTCC CTCCTGGGTC ACATGATCTC CGAGCTGACG 
AAGGCCAAAG ACGTGTATGA CACCAACTCT GAGGAAACAT CCGCTTTGAG CTCCAGTGTT 
TGGTCTCCTC AGTCCATTGA GTTCAGGCTC CAGCAGTTCT GTCTGCCCTT CCTGAGACTC 
TCCTGTTTAC TGCAGCACCA CCTGTATGGA GACAGCTTAC CCGGCTGCCT GGTGGAGGAG 
GAGTTCTCTC TGCTGACGCG CTGTCTGGGT TTAGCTGCTT CTGTTCAGTG CAGTGGATCC 
ATGAGCAGCG CAGCGTGTCT GGAGTGGAAC ATCAGTGCTT TTGACTTGAT CAGTCAGTGG 
TGCTCAGAAG TCGTCGCTCT GTCCGACACG CCCTCCCAGC AGTCTGCGTC TCTGTTAGGT 
CAGGACCCGC AGTGGGCTGC TCCTCGTCTC CTGCAGCTTC CCGACAATTA CAACACCATC 
TTCCAGTACT ACCACAGGAA ATCCTGCAGC AGCTGCGGAA AGACCCCCAA AGACCCTGCG 
CTGTGCCTGG TGTGCGGCGC GTTCGTCTGC CTGAAGGGTC ATTGCTGTAA GCAGCAGGGC 
GTCTGCGAGT GTGTGCTGCA CTCCCAGCAC TGCGGAGCGG CGACTGGTAT CTTCCTCCTG 
ATAAACGCAT CCGTCATCAT TATCATCCGA GGGCATCGCT TCTGCCTGTG GGGGTCTGTG 
TATCTGGATG CTCACGGAGA GGAAGACCGA GACCTACGTC GAGGAAAGCC TCTGTATTTG 
TGCGAGGAGA GATATCGTGT TCTGGAGCAG CAGTGGGTCG CTCACACCTT CGACCACATC 
AACAAGCGCT GGGGTCCTCA TTACAACGGA CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334500.1
CDS	74..5629
Translation	MMAASLLRRDKKSTAAHLKADLKRTDNSSGLRQLQELLDSVLNPERGSDPEALDWCKWLLAGGDGFDEFCRTVRSYDNATLCGLVWTANFVAYRCRTCGISPCMSLCAECFNNGDHTGHDFNMFRSQAGGACDCGDGNVMRESGFCNRHRLKTGENVPSVPRDLLLMSEMVLPRFIITIIQYLRDGYTEPESAADRDLQKVLQQLDPHISFLEELTKMGGAMRTVLTKILTDQQTFKELSMGQEDNVYAKKNYEKYLSALKSSGLVSVEEKGAAGGAGDGTSDAAAGAGALSLLGATATASLDDSSKEEDQDGLQGVGQRKRVKLSSSTKDPSIMDTLKHKCFLEELLFWTIKYEFPQKMVTFLLNMLPDQDYKITFTKTFVQHYAFIMKTLMKSHESDTMSNRIVHISVQLFSNEELARHVTEECQLLDIMVTVLLYMMESCLIKSELQDEENNRHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHSLLLLWMSFVSFFQGMNLNKRELNEHVEFESQTYYAAFAAELEACAQPMWGLLTHCKVKETQDYTKTVVRYCLETLQMWFDAIGFVDEPSLNQLTFHLPLHRYYAMFLSKGVKCQGLDLDSLLPDQEMLMKIMVHPLQIQASLSEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNAVLDSEQERPMLEGALTFLVILCSLRIHLGMSDDEILRAEMVSQLCMNDRTHSSLLDLIPENPNPKSGVVPGSCSFEEMLSGVADFKAPVFEPGGSMQQGMYTPKAEVWEKEFDPIMVILRTVYRRDVQSAMDRYSAFLKQSGVHTGNPWPPYKERTPLHPCYKGLVRLLHCKTLHIVIFTLLYKIWMDHQNMSEHVLCMVLYLIELGLDNQVQDDKVEEEPCIEEHCHDSWFPGTSLLSNLHHVINFVRVRVPETAPEVERKRERERERETPPSTSSESATFGQNLREAQVFSLVAERRRKFQEIINRSNHEASQAVRPKSSASRWLPPGTPPQLVTEILEIRESMLSLLVKLHQKLSAKQNSLSLSWLAEVDPAHHAHGDGLTAIERILAKASARSRHSKRCLQEICGKVCPPIPPKKISPGDKKSMDKEERRQRARERQQKLLAEFASRQKSFMETAMDVESPEADAVMDVSSEESLDSEVLYDCVICGQSGPSTEDRPTGLVVLLQASSVLGHRCRSDMPKRLPTTDEEHIYPEDTCGATHDVRLSLMQRYFKDSSCLQSVSIGWDGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGISVDKGEFTCPLCRQFANSVLPCRPGRGMETGAWHAPSTKSMSTLVKEVEDLQEQLGIFPIESNLSKEMESVIKDIKNTTQKKYMDYGKNPGSPDNDFLFMYSVARTNLELELVHRGGNLCSGGASAAAKRSCLNQLFHVLAMHMRLYSIDSAYNPWTRLTLSTQSRENEYCDEERPEVPMLFRDVPSLLIIFILTMPQPLRKEHFTCVVKVLYSLQFTQALAALSIRFSREERLAWSNTGAAKKNLPNSDKSWESLLGHMISELTKAKDVYDTNSEETSALSSSVWSPQSIEFRLQQFCLPFLRLSCLLQHHLYGDSLPGCLVEEEFSLLTRCLGLAASVQCSGSMSSAACLEWNISAFDLISQWCSEVVALSDTPSQQSASLLGQDPQWAAPRLLQLPDNYNTIFQYYHRKSCSSCGKTPKDPALCLVCGAFVCLKGHCCKQQGVCECVLHSQHCGAATGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYLCEERYRVLEQQWVAHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_021478825.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478825.1

ORF Insert Sequence:

ATGATGGCGG CGTCTCTGCT GCGCCGGGAC AAGAAATCCA CCGCCGCCCA TCTGAAGGCA 
GACCTCAAGC GGACTGACAA CAGTTCAGGC TTGCGGCAGC TGCAGGAACT GCTGGATAGC 
GTACTGAACC CGGAGCGGGG CTCGGACCCG GAGGCGCTGG ACTGGTGTAA ATGGCTGCTG 
GCCGGAGGGG ACGGTTTCGA CGAGTTCTGC CGGACCGTCC GATCCTACGA TAACGCCACC 
CTCTGCGGGC TGGTGTGGAC TGCCAACTTC GTGGCCTACC GTTGCCGGAC TTGCGGCATT 
TCCCCGTGCA TGTCGTTATG CGCGGAGTGC TTCAATAACG GCGACCACAC GGGCCATGAT 
TTTAACATGT TCCGGAGTCA GGCCGGCGGG GCCTGTGACT GCGGGGACGG GAACGTGATG 
AGAGAGAGCG GGTTTTGTAA CAGACATCGA TTAAAAACAG GAGAGAACGT TCCATCAGTC 
CCTCGAGATC TGCTGCTCAT GTCTGAAATG GTGCTGCCGC GGTTTATCAT CACTATCATT 
CAATATCTGA GAGACGGCTA CACTGAACCA GAGTCGGCCG CTGACAGAGA TCTTCAGAAG 
GTTCTGCAGC AGTTGGATCC ACACATCTCC TTCTTAGAAG AGCTCACCAA GATGGGCGGA 
GCCATGCGCA CCGTTCTCAC CAAGATCTTA ACCGATCAGC AGACCTTTAA AGAGCTCAGC 
ATGGGTCAAG AGGATAATGT TTATGCAAAG AAGAACTATG AGAAGTATTT GTCGGCATTG 
AAGAGCTCAG GTCTGGTGTC AGTGGAGGAG AAAGGAGCCG CTGGAGGAGC TGGAGACGGG 
ACATCAGATG CTGCAGCAGG GGCAGGAGCG CTCAGTCTAC TGGGAGCCAC TGCTACTGCT 
AGTCTGGATG ACTCCAGCAA AGAGGAGGAT CAGGATGGGC TGCAGGGTGT GGGTCAGAGG 
AAAAGAGTGA AGCTCAGTAG CAGTACCAAA GATCCATCCA TAATGGACAC GCTGAAACAT 
AAATGCTTTT TAGAAGAGTT ACTGTTTTGG ACTATTAAGT ATGAATTTCC ACAGAAGATG 
GTGACGTTTT TGCTCAACAT GTTGCCAGAT CAGGATTACA AGATCACCTT CACTAAAACA 
TTCGTACAAC ACTATGCCTT CATCATGAAG ACCCTCATGA AGAGTCACGA GTCAGACACC 
ATGTCCAACC GCATCGTGCA CATCAGCGTG CAGCTCTTCA GCAACGAGGA GCTGGCCAGA 
CACGTCACAG AGGAGTGCCA GCTACTGGAC ATCATGGTCA CCGTTCTCCT CTACATGATG 
GAGAGCTGCC TTATCAAGAG CGAACTGCAA GATGAGGAGA ATAATCGTCA TGTGGTGGTG 
AACTGTGGCG AAGCTCTGCT GAAGAATAAC ACCTACTGGC CTTTAGTTAG TGATTTTATT 
AATATCCTAT CACACCAAAG TGTGGCCAAG CGCTTCCTGG AGGATCATTC TCTGCTGCTG 
CTCTGGATGA GTTTTGTCTC CTTCTTTCAA GGAATGAATC TGAATAAGCG GGAGCTGAAT 
GAGCACGTGG AGTTTGAGTC TCAGACATAT TACGCAGCGT TTGCTGCTGA ACTGGAGGCC 
TGCGCTCAGC CCATGTGGGG TCTCCTCACA CACTGCAAAG TTAAAGAGAC CCAAGACTAC 
ACTAAGACAG TGGTGCGGTA CTGTCTGGAA ACTCTTCAGA TGTGGTTTGA TGCCATTGGG 
TTTGTAGATG AGCCTTCCCT CAACCAGCTG ACGTTTCATT TACCTCTTCA CCGATACTAC 
GCCATGTTCC TCAGCAAGGG AGTGAAGTGT CAAGGTCTGG ATTTAGACAG TTTGCTTCCT 
GATCAGGAGA TGCTGATGAA GATCATGGTT CACCCCCTAC AGATTCAGGC TAGCCTGTCT 
GAGATCCATA GTAACATGTG GGTGAGAAAT GGGCTGCAGA TTAAAGGTCA GGCCATGACC 
TATGTCCAGT CTCACTTCTG CAACTCCATG ATTGATCCGG ACATCTACCT CCTGCAGGTG 
TGCGCTTCTA GACTGGATCC AGATTACTTC ATTTCCTCAG TCTTCGAGAG GTTCAAGGTG 
GTGGATCTGC TGACGATGGC GTCTCAACAT CAGAACGCAG TGCTGGACTC TGAACAAGAG 
AGGCCCATGC TGGAGGGGGC GCTCACCTTC TTGGTCATAC TGTGCAGTCT CAGGATACAC 
TTAGGAATGT CTGACGATGA GATTTTGAGA GCCGAGATGG TGTCACAGTT GTGTATGAAT 
GATCGTACCC ACAGCTCTCT GCTGGATCTT ATCCCTGAGA ACCCGAACCC TAAGAGCGGT 
GTGGTGCCGG GCAGCTGTAG TTTTGAGGAG ATGCTGTCTG GTGTAGCGGA TTTTAAAGCC 
CCTGTGTTTG AGCCTGGAGG ATCCATGCAG CAGGGCATGT ACACACCTAA AGCGGAGGTT 
TGGGAAAAAG AGTTTGACCC TATAATGGTG ATTCTACGCA CCGTTTACCG CAGAGACGTG 
CAGTCGGCCA TGGATCGATA CTCAGCATTT TTGAAACAGT CAGGCGTTCA CACAGGGAAC 
CCTTGGCCAC CCTATAAGGA GAGGACTCCT TTGCATCCCT GCTATAAAGG ACTGGTGCGC 
CTGCTGCACT GCAAAACCTT ACATATCGTC ATCTTTACAC TGCTTTATAA GATATGGATG 
GATCATCAGA ACATGTCTGA GCATGTGTTG TGTATGGTGC TGTACCTGAT AGAGCTTGGA 
CTTGATAATC AGGTCCAGGA TGACAAAGTG GAAGAGGAGC CCTGTATCGA GGAGCACTGC 
CACGACAGCT GGTTCCCGGG CACCAGCCTG CTGTCTAACC TCCATCACGT CATCAACTTT 
GTGCGGGTTC GAGTGCCGGA GACGGCTCCG GAGGTGGAGA GGAAGCGAGA GAGGGAACGA 
GAGAGAGAAA CTCCACCCAG CACCAGTTCA GAGAGCGCCA CCTTTGGTCA GAATCTGCGT 
GAAGCTCAGG TGTTCAGTCT GGTGGCGGAG CGCAGGAGAA AGTTCCAGGA GATCATCAAC 
CGCAGCAACC ATGAGGCCAG TCAGGCAGTG CGCCCCAAGT CCTCAGCGTC CCGCTGGCTT 
CCACCGGGAA CGCCTCCACA GCTGGTGACC GAGATCCTGG AGATACGCGA GAGCATGCTG 
TCGCTGCTGG TTAAACTGCA CCAGAAACTG TCGGCCAAGC AGAACTCTCT GTCTCTGAGC 
TGGTTGGCCG AGGTGGATCC TGCCCATCAT GCGCACGGAG ACGGTTTAAC GGCCATCGAA 
AGAATCTTAG CCAAAGCTTC AGCTCGAAGC AGACACAGTA AAAGATGCCT ACAGGAGATC 
TGCGGGAAAG TGTGTCCACC CATACCGCCA AAGAAGATCA GTCCTGGAGA TAAGAAGAGC 
ATGGACAAGG AGGAGAGGCG TCAGCGCGCC AGAGAGAGGC AGCAGAAGCT GCTGGCGGAG 
TTTGCCTCCA GGCAGAAGAG CTTCATGGAG ACTGCCATGG ATGTTGAGTC CCCTGAAGCA 
GATGCTGTAA TGGACGTCAG CTCTGAGGAA TCTTTGGACT CTGAGGTTCT CTATGACTGT 
GTGATCTGTG GTCAGAGTGG ACCGTCCACT GAAGACAGAC CCACAGGACT GGTGGTTCTG 
CTGCAGGCTT CGTCAGTTTT GGGTCACCGT TGTCGTAGTG ACATGCCAAA GCGATTGCCC 
ACCACAGACG AAGAACACAT CTACCCTGAG GACACATGTG GAGCCACACA CGATGTCAGG 
CTGTCCCTTA TGCAACGCTA TTTTAAAGAT AGTTCCTGTC TGCAGTCTGT GTCTATTGGC 
TGGGACGGAG GGGTGTATGT GCAGACGTGT GGCCACACTC TGCATATAGA CTGTCACAAG 
TCCTACATGG AGTCCTTACG GAATGATCAG GTTCTTCAGG GGATTTCTGT GGATAAAGGC 
GAGTTCACTT GTCCGCTTTG CCGCCAGTTT GCAAACAGTG TTTTACCTTG TCGTCCTGGA 
CGAGGGATGG AAACCGGAGC CTGGCACGCA CCCAGTACCA AGAGCATGTC TACACTGGTG 
AAAGAAGTAG AAGACCTCCA GGAGCAGCTG GGCATTTTCC CAATCGAGTC AAACCTGAGT 
AAAGAGATGG AATCTGTTAT AAAGGACATT AAAAACACCA CACAGAAGAA ATACATGGAT 
TATGGGAAGA ACCCTGGGTC ACCTGACAAT GACTTCCTCT TCATGTACTC CGTGGCCAGA 
ACAAATCTGG AGCTTGAGTT GGTTCATCGT GGTGGGAATC TATGCAGTGG AGGGGCCAGT 
GCAGCTGCCA AACGCTCCTG TCTCAATCAG CTGTTTCACG TGCTGGCCAT GCACATGCGG 
CTCTACAGCA TAGACTCGGC ATACAATCCA TGGACCAGAC TCACACTGAG CACGCAAAGC 
AGAGAGAATG AGTACTGTGA TGAAGAGCGT CCTGAGGTTC CCATGCTTTT CCGAGACGTC 
CCATCCCTCC TAATCATCTT CATCCTCACC ATGCCTCAGC CTTTACGCAA AGAGCACTTC 
ACGTGTGTGG TGAAGGTGTT GTACAGTCTG CAGTTCACAC AGGCTCTGGC TGCGCTCTCC 
ATCAGGTTCA GCCGTGAGGA GAGACTCGCC TGGAGCAACA CTGGAGCTGC CAAGAAGAAC 
CTGCCAAACT CTGACAAATC ATGGGAGTCC CTCCTGGGTC ACATGATCTC CGAGCTGACG 
AAGGCCAAAG ACGTGTATGA CACCAACTCT GAGGAAACAT CCGCTTTGAG CTCCAGTGTT 
TGGTCTCCTC AGTCCATTGA GTTCAGGCTC CAGCAGTTCT GTCTGCCCTT CCTGAGACTC 
TCCTGTTTAC TGCAGCACCA CCTGTATGGA GACAGCTTAC CCGGCTGCCT GGTGGAGGAG 
GAGTTCTCTC TGCTGACGCG CTGTCTGGGT TTAGCTGCTT CTGTTCAGTG CAGTGGATCC 
ATGAGCAGCG CAGCGTGTCT GGAGTGGAAC ATCAGTGCTT TTGACTTGAT CAGTCAGTGG 
TGCTCAGAAG TCGTCGCTCT GTCCGACACG CCCTCCCAGC AGTCTGCGTC TCTGTTAGGT 
CAGGACCCGC AGTGGGCTGC TCCTCGTCTC CTGCAGCTTC CCGACAATTA CAACACCATC 
TTCCAGTACT ACCACAGGAA ATCCTGCAGC AGCTGCGGAA AGACCCCCAA AGACCCTGCG 
CTGTGCCTGG TGTGCGGCGC GTTCGTCTGC CTGAAGGGTC ATTGCTGTAA GCAGCAGGGC 
GTCTGCGAGT GTGTGCTGCA CTCCCAGCAC TGCGGAGCGG CGACTGGTAT CTTCCTCCTG 
ATAAACGCAT CCGTCATCAT TATCATCCGA GGGCATCGCT TCTGCCTGTG GGGGTCTGTG 
TATCTGGATG CTCACGGAGA GGAAGACCGA GACCTACGTC GAGGAAAGCC TCTGTATTTG 
TGCGAGGAGA GATATCGTGT TCTGGAGCAG CAGTGGGTCG CTCACACCTT CGACCACATC 
AACAAGCGCT GGGGTCCTCA TTACAACGGA CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ubr3 ( XM_017357738.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213227.1 Danio rerio ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa60284
Clone ID Related Accession (Same CDS sequence)	XM_017357738.2 , XM_017357738.1
Accession Version	XM_017357738.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase UBR3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357738.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG CGTCTCTGCT GCGCCGGGAC AAGAAATCCA CCGCCGCCCA TCTGAAGGCA 
GACCTCAAGC GGACTGACAA CAGTTCAGGC TTGCGGCAGC TGCAGGAACT GCTGGATAGC 
GTACTGAACC CGGAGCGGGG CTCGGACCCG GAGGCGCTGG ACTGGTGTAA ATGGCTGCTG 
GCCGGAGGGG ACGGTTTCGA CGAGTTCTGC CGGACCGTCC GATCCTACGA TAACGCCACC 
CTCTGCGGGC TGGTGTGGAC TGCCAACTTC GTGGCCTACC GTTGCCGGAC TTGCGGCATT 
TCCCCGTGCA TGTCGTTATG CGCGGAGTGC TTCAATAACG GCGACCACAC GGGCCATGAT 
TTTAACATGT TCCGGAGTCA GGCCGGCGGG GCCTGTGACT GCGGGGACGG GAACGTGATG 
AGAGAGAGCG GGTTTTGTAA CAGACATCGA TTAAAAACAG GAGAGAACGT TCCATCAGTC 
CCTCGAGATC TGCTGCTCAT GTCTGAAATG GTGCTGCCGC GGTTTATCAT CACTATCATT 
CAATATCTGA GAGACGGCTA CACTGAACCA GAGTCGGCCG CTGACAGAGA TCTTCAGAAG 
GTTCTGCAGC AGTTGGATCC ACACATCTCC TTCTTAGAAG AGCTCACCAA GATGGGCGGA 
GCCATGCGCA CCGTTCTCAC CAAGATCTTA ACCGATCAGC AGACCTTTAA AGAGCTCAGC 
ATGGGTCAAG AGGATAATGT TTATGCAAAG AAGAACTATG AGAAGTATTT GTCGGCATTG 
AAGAGCTCAG GTCTGGTGTC AGTGGAGGAG AAAGGAGCCG CTGGAGGAGC TGGAGACGGG 
ACATCAGATG CTGCAGCAGG GGCAGGAGCG CTCAGTCTAC TGGGAGCCAC TGCTACTGCT 
AGTCTGGATG ACTCCAGCAA AGAGGAGGAT CAGGATGGGC TGCAGGGTGT GGGTCAGAGG 
AAAAGAGTGA AGCTCAGTAG CAGTACCAAA GATCCATCCA TAATGGACAC GCTGAAACAT 
AAATGCTTTT TAGAAGAGTT ACTGTTTTGG ACTATTAAGT ATGAATTTCC ACAGAAGATG 
GTGACGTTTT TGCTCAACAT GTTGCCAGAT CAGGATTACA AGATCACCTT CACTAAAACA 
TTCGTACAAC ACTATGCCTT CATCATGAAG ACCCTCATGA AGAGTCACGA GTCAGACACC 
ATGTCCAACC GCATCGTGCA CATCAGCGTG CAGCTCTTCA GCAACGAGGA GCTGGCCAGA 
CACGTCACAG AGGAGTGCCA GCTACTGGAC ATCATGGTCA CCGTTCTCCT CTACATGATG 
GAGAGCTGCC TTATCAAGAG CGAACTGCAA GATGAGGAGA ATAATCGTCA TGTGGTGGTG 
AACTGTGGCG AAGCTCTGCT GAAGAATAAC ACCTACTGGC CTTTAGTTAG TGATTTTATT 
AATATCCTAT CACACCAAAG TGTGGCCAAG CGCTTCCTGG AGGATCATTC TCTGCTGCTG 
CTCTGGATGA GTTTTGTCTC CTTCTTTCAA GGAATGAATC TGAATAAGCG GGAGCTGAAT 
GAGCACGTGG AGTTTGAGTC TCAGACATAT TACGCAGCGT TTGCTGCTGA ACTGGAGGCC 
TGCGCTCAGC CCATGTGGGG TCTCCTCACA CACTGCAAAG TTAAAGAGAC CCAAGACTAC 
ACTAAGACAG TGGTGCGGTA CTGTCTGGAA ACTCTTCAGA TGTGGTTTGA TGCCATTGGG 
TTTGTAGATG AGCCTTCCCT CAACCAGCTG ACGTTTCATT TACCTCTTCA CCGATACTAC 
GCCATGTTCC TCAGCAAGGG AGTGAAGTGT CAAGGTCTGG ATTTAGACAG TTTGCTTCCT 
GATCAGGAGA TGCTGATGAA GATCATGGTT CACCCCCTAC AGATTCAGGC TAGCCTGTCT 
GAGATCCATA GTAACATGTG GGTGAGAAAT GGGCTGCAGA TTAAAGGTCA GGCCATGACC 
TATGTCCAGT CTCACTTCTG CAACTCCATG ATTGATCCGG ACATCTACCT CCTGCAGGTG 
TGCGCTTCTA GACTGGATCC AGATTACTTC ATTTCCTCAG TCTTCGAGAG GTTCAAGGTG 
GTGGATCTGC TGACGATGGC GTCTCAACAT CAGAACGCAG TGCTGGACTC TGAACAAGAG 
AGGCCCATGC TGGAGGGGGC GCTCACCTTC TTGGTCATAC TGTGCAGTCT CAGGATACAC 
TTAGGAATGT CTGACGATGA GATTTTGAGA GCCGAGATGG TGTCACAGTT GTGTATGAAT 
GATCGTACCC ACAGCTCTCT GCTGGATCTT ATCCCTGAGA ACCCGAACCC TAAGAGCGGT 
GTGGTGCCGG GCAGCTGTAG TTTTGAGGAG ATGCTGTCTG GTGTAGCGGA TTTTAAAGCC 
CCTGTGTTTG AGCCTGGAGG ATCCATGCAG CAGGGCATGT ACACACCTAA AGCGGAGGTT 
TGGGAAAAAG AGTTTGACCC TATAATGGTG ATTCTACGCA CCGTTTACCG CAGAGACGTG 
CAGTCGGCCA TGGATCGATA CTCAGCATTT TTGAAACAGT CAGGCGTTCA CACAGGGAAC 
CCTTGGCCAC CCTATAAGGA GAGGACTCCT TTGCATCCCT GCTATAAAGG ACTGGTGCGC 
CTGCTGCACT GCAAAACCTT ACATATCGTC ATCTTTACAC TGCTTTATAA GATATGGATG 
GATCATCAGA ACATGTCTGA GCATGTGTTG TGTATGGTGC TGTACCTGAT AGAGCTTGGA 
CTTGATAATC AGGTCCAGGA TGACAAAGTG GAAGAGGAGC CCTGTATCGA GGAGCACTGC 
CACGACAGCT GGTTCCCGGG CACCAGCCTG CTGTCTAACC TCCATCACGT CATCAACTTT 
GTGCGGGTTC GAGTGCCGGA GACGGCTCCG GAGGTGGAGA GGAAGCGAGA GAGGGAACGA 
GAGAGAGAAA CTCCACCCAG CACCAGTTCA GAGAGCGCCA CCTTTGGTCA GAATCTGCGT 
GAAGCTCAGG TGTTCAGTCT GGTGGCGGAG CGCAGGAGAA AGTTCCAGGA GATCATCAAC 
CGCAGCAACC ATGAGGCCAG TCAGGCAGTG CGCCCCAAGT CCTCAGCGTC CCGCTGGCTT 
CCACCGGGAA CGCCTCCACA GCTGGTGACC GAGATCCTGG AGATACGCGA GAGCATGCTG 
TCGCTGCTGG TTAAACTGCA CCAGAAACTG TCGGCCAAGC AGAACTCTCT GTCTCTGAGC 
TGGTTGGCCG AGGTGGATCC TGCCCATCAT GCGCACGGAG ACGGTTTAAC GGCCATCGAA 
AGAATCTTAG CCAAAGCTTC AGCTCGAAGC AGACACAGTA AAAGATGCCT ACAGGAGATC 
TGCGGGAAAG TGTGTCCACC CATACCGCCA AAGAAGATCA GTCCTGGAGA TAAGAAGAGC 
ATGGACAAGG AGGAGAGGCG TCAGCGCGCC AGAGAGAGGC AGCAGAAGCT GCTGGCGGAG 
TTTGCCTCCA GGCAGAAGAG CTTCATGGAG ACTGCCATGG ATGTTGAGTC CCCTGAAGCA 
GATGCTGTAA TGGACGTCAG CTCTGAGGAA TCTTTGGACT CTGAGGTTCT CTATGACTGT 
GTGATCTGTG GTCAGAGTGG ACCGTCCACT GAAGACAGAC CCACAGGACT GGTGGTTCTG 
CTGCAGGCTT CGTCAGTTTT GGGTCACCGT TGTCGTAGTG ACATGCCAAA GCGATTGCCC 
ACCACAGACG AAGAACACAT CTACCCTGAG GACACATGTG GAGCCACACA CGATGTCAGG 
CTGTCCCTTA TGCAACGCTA TTTTAAAGAT AGTTCCTGTC TGCAGTCTGT GTCTATTGGC 
TGGGACGGAG GGGTGTATGT GCAGACGTGT GGCCACACTC TGCATATAGA CTGTCACAAG 
TCCTACATGG AGTCCTTACG GAATGATCAG GTTCTTCAGG GGATTTCTGT GGATAAAGGC 
GAGTTCACTT GTCCGCTTTG CCGCCAGTTT GCAAACAGTG TTTTACCTTG TCGTCCTGGA 
CGAGGGATGG AAACCGGAGC CTGGCACGCA CCCAGTACCA AGAGCATGTC TACACTGGTG 
AAAGAAGTAG AAGACCTCCA GGAGCAGCTG GGCATTTTCC CAGTAAGAGC CAGCATAAAG 
CAAAGAGATC CCATTCTGAT CGAGTCAAAC CTGAGTAAAG AGATGGAATC TGTTATAAAG 
GACATTAAAA ACACCACACA GAAGAAATAC ATGGATTATG GGAAGAACCC TGGGTCACCT 
GACAATGACT TCCTCTTCAT GTACTCCGTG GCCAGAACAA ATCTGGAGCT TGAGTTGGTT 
CATCGTGGTG GGAATCTATG CAGTGGAGGG GCCAGTGCAG CTGCCAAACG CTCCTGTCTC 
AATCAGCTGT TTCACGTGCT GGCCATGCAC ATGCGGCTCT ACAGCATAGA CTCGGCATAC 
AATCCATGGA CCAGACTCAC ACTGAGCACG CAAAGCAGAG AGAATGAGTA CTGTGATGAA 
GAGCGTCCTG AGGTTCCCAT GCTTTTCCGA GACGTCCCAT CCCTCCTAAT CATCTTCATC 
CTCACCATGC CTCAGCCTTT ACGCAAAGAG CACTTCACGT GTGTGGTGAA GGTGTTGTAC 
AGTCTGCAGT TCACACAGGC TCTGGCTGCG CTCTCCATCA GGTTCAGCCG TGAGGAGAGA 
CTCGCCTGGA GCAACACTGG AGCTGCCAAG AAGAACCTGC CAAACTCTGA CAAATCATGG 
GAGTCCCTCC TGGGTCACAT GATCTCCGAG CTGACGAAGG CCAAAGACGT GTATGACACC 
AACTCTGAGG AAACATCCGC TTTGAGCTCC AGTGTTTGGT CTCCTCAGTC CATTGAGTTC 
AGGCTCCAGC AGTTCTGTCT GCCCTTCCTG AGACTCTCCT GTTTACTGCA GCACCACCTG 
TATGGAGACA GCTTACCCGG CTGCCTGGTG GAGGAGGAGT TCTCTCTGCT GACGCGCTGT 
CTGGGTTTAG CTGCTTCTGT TCAGTGCAGT GGATCCATGA GCAGCGCAGC GTGTCTGGAG 
TGGAACATCA GTGCTTTTGA CTTGATCAGT CAGTGGTGCT CAGAAGTCGT CGCTCTGTCC 
GACACGCCCT CCCAGCAGTC TGCGTCTCTG TTAGGTCAGG ACCCGCAGTG GGCTGCTCCT 
CGTCTCCTGC AGCTTCCCGA CAATTACAAC ACCATCTTCC AGTACTACCA CAGGAAATCC 
TGCAGCAGCT GCGGAAAGAC CCCCAAAGAC CCTGCGCTGT GCCTGGTGTG CGGCGCGTTC 
GTCTGCCTGA AGGGTCATTG CTGTAAGCAG CAGGGCGTCT GCGAGTGTGT GCTGCACTCC 
CAGCACTGCG GAGCGGCGAC TGGTATCTTC CTCCTGATAA ACGCATCCGT CATCATTATC 
ATCCGAGGGC ATCGCTTCTG CCTGTGGGGG TCTGTGTATC TGGATGCTCA CGGAGAGGAA 
GACCGAGACC TACGTCGAGG AAAGCCTCTG TATTTGTGCG AGGAGAGATA TCGTGTTCTG 
GAGCAGCAGT GGGTCGCTCA CACCTTCGAC CACATCAACA AGCGCTGGGG TCCTCATTAC 
AACGGACTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213227.1
CDS	74..5665
Translation	MMAASLLRRDKKSTAAHLKADLKRTDNSSGLRQLQELLDSVLNPERGSDPEALDWCKWLLAGGDGFDEFCRTVRSYDNATLCGLVWTANFVAYRCRTCGISPCMSLCAECFNNGDHTGHDFNMFRSQAGGACDCGDGNVMRESGFCNRHRLKTGENVPSVPRDLLLMSEMVLPRFIITIIQYLRDGYTEPESAADRDLQKVLQQLDPHISFLEELTKMGGAMRTVLTKILTDQQTFKELSMGQEDNVYAKKNYEKYLSALKSSGLVSVEEKGAAGGAGDGTSDAAAGAGALSLLGATATASLDDSSKEEDQDGLQGVGQRKRVKLSSSTKDPSIMDTLKHKCFLEELLFWTIKYEFPQKMVTFLLNMLPDQDYKITFTKTFVQHYAFIMKTLMKSHESDTMSNRIVHISVQLFSNEELARHVTEECQLLDIMVTVLLYMMESCLIKSELQDEENNRHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHSLLLLWMSFVSFFQGMNLNKRELNEHVEFESQTYYAAFAAELEACAQPMWGLLTHCKVKETQDYTKTVVRYCLETLQMWFDAIGFVDEPSLNQLTFHLPLHRYYAMFLSKGVKCQGLDLDSLLPDQEMLMKIMVHPLQIQASLSEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNAVLDSEQERPMLEGALTFLVILCSLRIHLGMSDDEILRAEMVSQLCMNDRTHSSLLDLIPENPNPKSGVVPGSCSFEEMLSGVADFKAPVFEPGGSMQQGMYTPKAEVWEKEFDPIMVILRTVYRRDVQSAMDRYSAFLKQSGVHTGNPWPPYKERTPLHPCYKGLVRLLHCKTLHIVIFTLLYKIWMDHQNMSEHVLCMVLYLIELGLDNQVQDDKVEEEPCIEEHCHDSWFPGTSLLSNLHHVINFVRVRVPETAPEVERKRERERERETPPSTSSESATFGQNLREAQVFSLVAERRRKFQEIINRSNHEASQAVRPKSSASRWLPPGTPPQLVTEILEIRESMLSLLVKLHQKLSAKQNSLSLSWLAEVDPAHHAHGDGLTAIERILAKASARSRHSKRCLQEICGKVCPPIPPKKISPGDKKSMDKEERRQRARERQQKLLAEFASRQKSFMETAMDVESPEADAVMDVSSEESLDSEVLYDCVICGQSGPSTEDRPTGLVVLLQASSVLGHRCRSDMPKRLPTTDEEHIYPEDTCGATHDVRLSLMQRYFKDSSCLQSVSIGWDGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGISVDKGEFTCPLCRQFANSVLPCRPGRGMETGAWHAPSTKSMSTLVKEVEDLQEQLGIFPVRASIKQRDPILIESNLSKEMESVIKDIKNTTQKKYMDYGKNPGSPDNDFLFMYSVARTNLELELVHRGGNLCSGGASAAAKRSCLNQLFHVLAMHMRLYSIDSAYNPWTRLTLSTQSRENEYCDEERPEVPMLFRDVPSLLIIFILTMPQPLRKEHFTCVVKVLYSLQFTQALAALSIRFSREERLAWSNTGAAKKNLPNSDKSWESLLGHMISELTKAKDVYDTNSEETSALSSSVWSPQSIEFRLQQFCLPFLRLSCLLQHHLYGDSLPGCLVEEEFSLLTRCLGLAASVQCSGSMSSAACLEWNISAFDLISQWCSEVVALSDTPSQQSASLLGQDPQWAAPRLLQLPDNYNTIFQYYHRKSCSSCGKTPKDPALCLVCGAFVCLKGHCCKQQGVCECVLHSQHCGAATGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYLCEERYRVLEQQWVAHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_017357738.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3 component n-recognin 3 (ubr3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357738.2

ORF Insert Sequence:

ATGATGGCGG CGTCTCTGCT GCGCCGGGAC AAGAAATCCA CCGCCGCCCA TCTGAAGGCA 
GACCTCAAGC GGACTGACAA CAGTTCAGGC TTGCGGCAGC TGCAGGAACT GCTGGATAGC 
GTACTGAACC CGGAGCGGGG CTCGGACCCG GAGGCGCTGG ACTGGTGTAA ATGGCTGCTG 
GCCGGAGGGG ACGGTTTCGA CGAGTTCTGC CGGACCGTCC GATCCTACGA TAACGCCACC 
CTCTGCGGGC TGGTGTGGAC TGCCAACTTC GTGGCCTACC GTTGCCGGAC TTGCGGCATT 
TCCCCGTGCA TGTCGTTATG CGCGGAGTGC TTCAATAACG GCGACCACAC GGGCCATGAT 
TTTAACATGT TCCGGAGTCA GGCCGGCGGG GCCTGTGACT GCGGGGACGG GAACGTGATG 
AGAGAGAGCG GGTTTTGTAA CAGACATCGA TTAAAAACAG GAGAGAACGT TCCATCAGTC 
CCTCGAGATC TGCTGCTCAT GTCTGAAATG GTGCTGCCGC GGTTTATCAT CACTATCATT 
CAATATCTGA GAGACGGCTA CACTGAACCA GAGTCGGCCG CTGACAGAGA TCTTCAGAAG 
GTTCTGCAGC AGTTGGATCC ACACATCTCC TTCTTAGAAG AGCTCACCAA GATGGGCGGA 
GCCATGCGCA CCGTTCTCAC CAAGATCTTA ACCGATCAGC AGACCTTTAA AGAGCTCAGC 
ATGGGTCAAG AGGATAATGT TTATGCAAAG AAGAACTATG AGAAGTATTT GTCGGCATTG 
AAGAGCTCAG GTCTGGTGTC AGTGGAGGAG AAAGGAGCCG CTGGAGGAGC TGGAGACGGG 
ACATCAGATG CTGCAGCAGG GGCAGGAGCG CTCAGTCTAC TGGGAGCCAC TGCTACTGCT 
AGTCTGGATG ACTCCAGCAA AGAGGAGGAT CAGGATGGGC TGCAGGGTGT GGGTCAGAGG 
AAAAGAGTGA AGCTCAGTAG CAGTACCAAA GATCCATCCA TAATGGACAC GCTGAAACAT 
AAATGCTTTT TAGAAGAGTT ACTGTTTTGG ACTATTAAGT ATGAATTTCC ACAGAAGATG 
GTGACGTTTT TGCTCAACAT GTTGCCAGAT CAGGATTACA AGATCACCTT CACTAAAACA 
TTCGTACAAC ACTATGCCTT CATCATGAAG ACCCTCATGA AGAGTCACGA GTCAGACACC 
ATGTCCAACC GCATCGTGCA CATCAGCGTG CAGCTCTTCA GCAACGAGGA GCTGGCCAGA 
CACGTCACAG AGGAGTGCCA GCTACTGGAC ATCATGGTCA CCGTTCTCCT CTACATGATG 
GAGAGCTGCC TTATCAAGAG CGAACTGCAA GATGAGGAGA ATAATCGTCA TGTGGTGGTG 
AACTGTGGCG AAGCTCTGCT GAAGAATAAC ACCTACTGGC CTTTAGTTAG TGATTTTATT 
AATATCCTAT CACACCAAAG TGTGGCCAAG CGCTTCCTGG AGGATCATTC TCTGCTGCTG 
CTCTGGATGA GTTTTGTCTC CTTCTTTCAA GGAATGAATC TGAATAAGCG GGAGCTGAAT 
GAGCACGTGG AGTTTGAGTC TCAGACATAT TACGCAGCGT TTGCTGCTGA ACTGGAGGCC 
TGCGCTCAGC CCATGTGGGG TCTCCTCACA CACTGCAAAG TTAAAGAGAC CCAAGACTAC 
ACTAAGACAG TGGTGCGGTA CTGTCTGGAA ACTCTTCAGA TGTGGTTTGA TGCCATTGGG 
TTTGTAGATG AGCCTTCCCT CAACCAGCTG ACGTTTCATT TACCTCTTCA CCGATACTAC 
GCCATGTTCC TCAGCAAGGG AGTGAAGTGT CAAGGTCTGG ATTTAGACAG TTTGCTTCCT 
GATCAGGAGA TGCTGATGAA GATCATGGTT CACCCCCTAC AGATTCAGGC TAGCCTGTCT 
GAGATCCATA GTAACATGTG GGTGAGAAAT GGGCTGCAGA TTAAAGGTCA GGCCATGACC 
TATGTCCAGT CTCACTTCTG CAACTCCATG ATTGATCCGG ACATCTACCT CCTGCAGGTG 
TGCGCTTCTA GACTGGATCC AGATTACTTC ATTTCCTCAG TCTTCGAGAG GTTCAAGGTG 
GTGGATCTGC TGACGATGGC GTCTCAACAT CAGAACGCAG TGCTGGACTC TGAACAAGAG 
AGGCCCATGC TGGAGGGGGC GCTCACCTTC TTGGTCATAC TGTGCAGTCT CAGGATACAC 
TTAGGAATGT CTGACGATGA GATTTTGAGA GCCGAGATGG TGTCACAGTT GTGTATGAAT 
GATCGTACCC ACAGCTCTCT GCTGGATCTT ATCCCTGAGA ACCCGAACCC TAAGAGCGGT 
GTGGTGCCGG GCAGCTGTAG TTTTGAGGAG ATGCTGTCTG GTGTAGCGGA TTTTAAAGCC 
CCTGTGTTTG AGCCTGGAGG ATCCATGCAG CAGGGCATGT ACACACCTAA AGCGGAGGTT 
TGGGAAAAAG AGTTTGACCC TATAATGGTG ATTCTACGCA CCGTTTACCG CAGAGACGTG 
CAGTCGGCCA TGGATCGATA CTCAGCATTT TTGAAACAGT CAGGCGTTCA CACAGGGAAC 
CCTTGGCCAC CCTATAAGGA GAGGACTCCT TTGCATCCCT GCTATAAAGG ACTGGTGCGC 
CTGCTGCACT GCAAAACCTT ACATATCGTC ATCTTTACAC TGCTTTATAA GATATGGATG 
GATCATCAGA ACATGTCTGA GCATGTGTTG TGTATGGTGC TGTACCTGAT AGAGCTTGGA 
CTTGATAATC AGGTCCAGGA TGACAAAGTG GAAGAGGAGC CCTGTATCGA GGAGCACTGC 
CACGACAGCT GGTTCCCGGG CACCAGCCTG CTGTCTAACC TCCATCACGT CATCAACTTT 
GTGCGGGTTC GAGTGCCGGA GACGGCTCCG GAGGTGGAGA GGAAGCGAGA GAGGGAACGA 
GAGAGAGAAA CTCCACCCAG CACCAGTTCA GAGAGCGCCA CCTTTGGTCA GAATCTGCGT 
GAAGCTCAGG TGTTCAGTCT GGTGGCGGAG CGCAGGAGAA AGTTCCAGGA GATCATCAAC 
CGCAGCAACC ATGAGGCCAG TCAGGCAGTG CGCCCCAAGT CCTCAGCGTC CCGCTGGCTT 
CCACCGGGAA CGCCTCCACA GCTGGTGACC GAGATCCTGG AGATACGCGA GAGCATGCTG 
TCGCTGCTGG TTAAACTGCA CCAGAAACTG TCGGCCAAGC AGAACTCTCT GTCTCTGAGC 
TGGTTGGCCG AGGTGGATCC TGCCCATCAT GCGCACGGAG ACGGTTTAAC GGCCATCGAA 
AGAATCTTAG CCAAAGCTTC AGCTCGAAGC AGACACAGTA AAAGATGCCT ACAGGAGATC 
TGCGGGAAAG TGTGTCCACC CATACCGCCA AAGAAGATCA GTCCTGGAGA TAAGAAGAGC 
ATGGACAAGG AGGAGAGGCG TCAGCGCGCC AGAGAGAGGC AGCAGAAGCT GCTGGCGGAG 
TTTGCCTCCA GGCAGAAGAG CTTCATGGAG ACTGCCATGG ATGTTGAGTC CCCTGAAGCA 
GATGCTGTAA TGGACGTCAG CTCTGAGGAA TCTTTGGACT CTGAGGTTCT CTATGACTGT 
GTGATCTGTG GTCAGAGTGG ACCGTCCACT GAAGACAGAC CCACAGGACT GGTGGTTCTG 
CTGCAGGCTT CGTCAGTTTT GGGTCACCGT TGTCGTAGTG ACATGCCAAA GCGATTGCCC 
ACCACAGACG AAGAACACAT CTACCCTGAG GACACATGTG GAGCCACACA CGATGTCAGG 
CTGTCCCTTA TGCAACGCTA TTTTAAAGAT AGTTCCTGTC TGCAGTCTGT GTCTATTGGC 
TGGGACGGAG GGGTGTATGT GCAGACGTGT GGCCACACTC TGCATATAGA CTGTCACAAG 
TCCTACATGG AGTCCTTACG GAATGATCAG GTTCTTCAGG GGATTTCTGT GGATAAAGGC 
GAGTTCACTT GTCCGCTTTG CCGCCAGTTT GCAAACAGTG TTTTACCTTG TCGTCCTGGA 
CGAGGGATGG AAACCGGAGC CTGGCACGCA CCCAGTACCA AGAGCATGTC TACACTGGTG 
AAAGAAGTAG AAGACCTCCA GGAGCAGCTG GGCATTTTCC CAGTAAGAGC CAGCATAAAG 
CAAAGAGATC CCATTCTGAT CGAGTCAAAC CTGAGTAAAG AGATGGAATC TGTTATAAAG 
GACATTAAAA ACACCACACA GAAGAAATAC ATGGATTATG GGAAGAACCC TGGGTCACCT 
GACAATGACT TCCTCTTCAT GTACTCCGTG GCCAGAACAA ATCTGGAGCT TGAGTTGGTT 
CATCGTGGTG GGAATCTATG CAGTGGAGGG GCCAGTGCAG CTGCCAAACG CTCCTGTCTC 
AATCAGCTGT TTCACGTGCT GGCCATGCAC ATGCGGCTCT ACAGCATAGA CTCGGCATAC 
AATCCATGGA CCAGACTCAC ACTGAGCACG CAAAGCAGAG AGAATGAGTA CTGTGATGAA 
GAGCGTCCTG AGGTTCCCAT GCTTTTCCGA GACGTCCCAT CCCTCCTAAT CATCTTCATC 
CTCACCATGC CTCAGCCTTT ACGCAAAGAG CACTTCACGT GTGTGGTGAA GGTGTTGTAC 
AGTCTGCAGT TCACACAGGC TCTGGCTGCG CTCTCCATCA GGTTCAGCCG TGAGGAGAGA 
CTCGCCTGGA GCAACACTGG AGCTGCCAAG AAGAACCTGC CAAACTCTGA CAAATCATGG 
GAGTCCCTCC TGGGTCACAT GATCTCCGAG CTGACGAAGG CCAAAGACGT GTATGACACC 
AACTCTGAGG AAACATCCGC TTTGAGCTCC AGTGTTTGGT CTCCTCAGTC CATTGAGTTC 
AGGCTCCAGC AGTTCTGTCT GCCCTTCCTG AGACTCTCCT GTTTACTGCA GCACCACCTG 
TATGGAGACA GCTTACCCGG CTGCCTGGTG GAGGAGGAGT TCTCTCTGCT GACGCGCTGT 
CTGGGTTTAG CTGCTTCTGT TCAGTGCAGT GGATCCATGA GCAGCGCAGC GTGTCTGGAG 
TGGAACATCA GTGCTTTTGA CTTGATCAGT CAGTGGTGCT CAGAAGTCGT CGCTCTGTCC 
GACACGCCCT CCCAGCAGTC TGCGTCTCTG TTAGGTCAGG ACCCGCAGTG GGCTGCTCCT 
CGTCTCCTGC AGCTTCCCGA CAATTACAAC ACCATCTTCC AGTACTACCA CAGGAAATCC 
TGCAGCAGCT GCGGAAAGAC CCCCAAAGAC CCTGCGCTGT GCCTGGTGTG CGGCGCGTTC 
GTCTGCCTGA AGGGTCATTG CTGTAAGCAG CAGGGCGTCT GCGAGTGTGT GCTGCACTCC 
CAGCACTGCG GAGCGGCGAC TGGTATCTTC CTCCTGATAA ACGCATCCGT CATCATTATC 
ATCCGAGGGC ATCGCTTCTG CCTGTGGGGG TCTGTGTATC TGGATGCTCA CGGAGAGGAA 
GACCGAGACC TACGTCGAGG AAAGCCTCTG TATTTGTGCG AGGAGAGATA TCGTGTTCTG 
GAGCAGCAGT GGGTCGCTCA CACCTTCGAC CACATCAACA AGCGCTGGGG TCCTCATTAC 
AACGGACTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ubr3 ( XM_017357738.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213227.1 Danio rerio ubiquitin protein ligase E3 component n-recognin 3 (ubr3), mRNA.

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CloneID	ODa60284
Clone ID Related Accession (Same CDS sequence)	XM_017357738.2 , XM_017357738.1
Accession Version	XM_017357738.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase UBR3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) and transcript sequence (GDQH01007031.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 142 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGATGGCGG CGTCTCTGCT GCGCCGGGAC AAGAAATCCA CCGCCGCCCA TCTGAAGGCA 
GACCTCAAGC GGACTGACAA CAGTTCAGGC TTGCGGCAGC TGCAGGAACT GCTGGATAGC 
GTACTGAACC CGGAGCGGGG CTCGGACCCG GAGGCGCTGG ACTGGTGTAA ATGGCTGCTG 
GCCGGAGGGG ACGGTTTCGA CGAGTTCTGC CGGACCGTCC GATCCTACGA TAACGCCACC 
CTCTGCGGGC TGGTGTGGAC TGCCAACTTC GTGGCCTACC GTTGCCGGAC TTGCGGCATT 
TCCCCGTGCA TGTCGTTATG CGCGGAGTGC TTCAATAACG GCGACCACAC GGGCCATGAT 
TTTAACATGT TCCGGAGTCA GGCCGGCGGG GCCTGTGACT GCGGGGACGG GAACGTGATG 
AGAGAGAGCG GGTTTTGTAA CAGACATCGA TTAAAAACAG GAGAGAACGT TCCATCAGTC 
CCTCGAGATC TGCTGCTCAT GTCTGAAATG GTGCTGCCGC GGTTTATCAT CACTATCATT 
CAATATCTGA GAGACGGCTA CACTGAACCA GAGTCGGCCG CTGACAGAGA TCTTCAGAAG 
GTTCTGCAGC AGTTGGATCC ACACATCTCC TTCTTAGAAG AGCTCACCAA GATGGGCGGA 
GCCATGCGCA CCGTTCTCAC CAAGATCTTA ACCGATCAGC AGACCTTTAA AGAGCTCAGC 
ATGGGTCAAG AGGATAATGT TTATGCAAAG AAGAACTATG AGAAGTATTT GTCGGCATTG 
AAGAGCTCAG GTCTGGTGTC AGTGGAGGAG AAAGGAGCCG CTGGAGGAGC TGGAGACGGG 
ACATCAGATG CTGCAGCAGG GGCAGGAGCG CTCAGTCTAC TGGGAGCCAC TGCTACTGCT 
AGTCTGGATG ACTCCAGCAA AGAGGAGGAT CAGGATGGGC TGCAGGGTGT GGGTCAGAGG 
AAAAGAGTGA AGCTCAGTAG CAGTACCAAA GATCCATCCA TAATGGACAC GCTGAAACAT 
AAATGCTTTT TAGAAGAGTT ACTGTTTTGG ACTATTAAGT ATGAATTTCC ACAGAAGATG 
GTGACGTTTT TGCTCAACAT GTTGCCAGAT CAGGATTACA AGATCACCTT CACTAAAACA 
TTCGTACAAC ACTATGCCTT CATCATGAAG ACCCTCATGA AGAGTCACGA GTCAGACACC 
ATGTCCAACC GCATCGTGCA CATCAGCGTG CAGCTCTTCA GCAACGAGGA GCTGGCCAGA 
CACGTCACAG AGGAGTGCCA GCTACTGGAC ATCATGGTCA CCGTTCTCCT CTACATGATG 
GAGAGCTGCC TTATCAAGAG CGAACTGCAA GATGAGGAGA ATAATCGTCA TGTGGTGGTG 
AACTGTGGCG AAGCTCTGCT GAAGAATAAC ACCTACTGGC CTTTAGTTAG TGATTTTATT 
AATATCCTAT CACACCAAAG TGTGGCCAAG CGCTTCCTGG AGGATCATTC TCTGCTGCTG 
CTCTGGATGA GTTTTGTCTC CTTCTTTCAA GGAATGAATC TGAATAAGCG GGAGCTGAAT 
GAGCACGTGG AGTTTGAGTC TCAGACATAT TACGCAGCGT TTGCTGCTGA ACTGGAGGCC 
TGCGCTCAGC CCATGTGGGG TCTCCTCACA CACTGCAAAG TTAAAGAGAC CCAAGACTAC 
ACTAAGACAG TGGTGCGGTA CTGTCTGGAA ACTCTTCAGA TGTGGTTTGA TGCCATTGGG 
TTTGTAGATG AGCCTTCCCT CAACCAGCTG ACGTTTCATT TACCTCTTCA CCGATACTAC 
GCCATGTTCC TCAGCAAGGG AGTGAAGTGT CAAGGTCTGG ATTTAGACAG TTTGCTTCCT 
GATCAGGAGA TGCTGATGAA GATCATGGTT CACCCCCTAC AGATTCAGGC TAGCCTGTCT 
GAGATCCATA GTAACATGTG GGTGAGAAAT GGGCTGCAGA TTAAAGGTCA GGCCATGACC 
TATGTCCAGT CTCACTTCTG CAACTCCATG ATTGATCCGG ACATCTACCT CCTGCAGGTG 
TGCGCTTCTA GACTGGATCC AGATTACTTC ATTTCCTCAG TCTTCGAGAG GTTCAAGGTG 
GTGGATCTGC TGACGATGGC GTCTCAACAT CAGAACGCAG TGCTGGACTC TGAACAAGAG 
AGGCCCATGC TGGAGGGGGC GCTCACCTTC TTGGTCATAC TGTGCAGTCT CAGGATACAC 
TTAGGAATGT CTGACGATGA GATTTTGAGA GCCGAGATGG TGTCACAGTT GTGTATGAAT 
GATCGTACCC ACAGCTCTCT GCTGGATCTT ATCCCTGAGA ACCCGAACCC TAAGAGCGGT 
GTGGTGCCGG GCAGCTGTAG TTTTGAGGAG ATGCTGTCTG GTGTAGCGGA TTTTAAAGCC 
CCTGTGTTTG AGCCTGGAGG ATCCATGCAG CAGGGCATGT ACACACCTAA AGCGGAGGTT 
TGGGAAAAAG AGTTTGACCC TATAATGGTG ATTCTACGCA CCGTTTACCG CAGAGACGTG 
CAGTCGGCCA TGGATCGATA CTCAGCATTT TTGAAACAGT CAGGCGTTCA CACAGGGAAC 
CCTTGGCCAC CCTATAAGGA GAGGACTCCT TTGCATCCCT GCTATAAAGG ACTGGTGCGC 
CTGCTGCACT GCAAAACCTT ACATATCGTC ATCTTTACAC TGCTTTATAA GATATGGATG 
GATCATCAGA ACATGTCTGA GCATGTGTTG TGTATGGTGC TGTACCTGAT AGAGCTTGGA 
CTTGATAATC AGGTCCAGGA TGACAAAGTG GAAGAGGAGC CCTGTATCGA GGAGCACTGC 
CACGACAGCT GGTTCCCGGG CACCAGCCTG CTGTCTAACC TCCATCACGT CATCAACTTT 
GTGCGGGTTC GAGTGCCGGA GACGGCTCCG GAGGTGGAGA GGAAGCGAGA GAGGGAACGA 
GAGAGAGAAA CTCCACCCAG CACCAGTTCA GAGAGCGCCA CCTTTGGTCA GAATCTGCGT 
GAAGCTCAGG TGTTCAGTCT GGTGGCGGAG CGCAGGAGAA AGTTCCAGGA GATCATCAAC 
CGCAGCAACC ATGAGGCCAG TCAGGCAGTG CGCCCCAAGT CCTCAGCGTC CCGCTGGCTT 
CCACCGGGAA CGCCTCCACA GCTGGTGACC GAGATCCTGG AGATACGCGA GAGCATGCTG 
TCGCTGCTGG TTAAACTGCA CCAGAAACTG TCGGCCAAGC AGAACTCTCT GTCTCTGAGC 
TGGTTGGCCG AGGTGGATCC TGCCCATCAT GCGCACGGAG ACGGTTTAAC GGCCATCGAA 
AGAATCTTAG CCAAAGCTTC AGCTCGAAGC AGACACAGTA AAAGATGCCT ACAGGAGATC 
TGCGGGAAAG TGTGTCCACC CATACCGCCA AAGAAGATCA GTCCTGGAGA TAAGAAGAGC 
ATGGACAAGG AGGAGAGGCG TCAGCGCGCC AGAGAGAGGC AGCAGAAGCT GCTGGCGGAG 
TTTGCCTCCA GGCAGAAGAG CTTCATGGAG ACTGCCATGG ATGTTGAGTC CCCTGAAGCA 
GATGCTGTAA TGGACGTCAG CTCTGAGGAA TCTTTGGACT CTGAGGTTCT CTATGACTGT 
GTGATCTGTG GTCAGAGTGG ACCGTCCACT GAAGACAGAC CCACAGGACT GGTGGTTCTG 
CTGCAGGCTT CGTCAGTTTT GGGTCACCGT TGTCGTAGTG ACATGCCAAA GCGATTGCCC 
ACCACAGACG AAGAACACAT CTACCCTGAG GACACATGTG GAGCCACACA CGATGTCAGG 
CTGTCCCTTA TGCAACGCTA TTTTAAAGAT AGTTCCTGTC TGCAGTCTGT GTCTATTGGC 
TGGGACGGAG GGGTGTATGT GCAGACGTGT GGCCACACTC TGCATATAGA CTGTCACAAG 
TCCTACATGG AGTCCTTACG GAATGATCAG GTTCTTCAGG GGATTTCTGT GGATAAAGGC 
GAGTTCACTT GTCCGCTTTG CCGCCAGTTT GCAAACAGTG TTTTACCTTG TCGTCCTGGA 
CGAGGGATGG AAACCGGAGC CTGGCACGCA CCCAGTACCA AGAGCATGTC TACACTGGTG 
AAAGAAGTAG AAGACCTCCA GGAGCAGCTG GGCATTTTCC CAGTAAGAGC CAGCATAAAG 
CAAAGAGATC CCATTCTGAT CGAGTCAAAC CTGAGTAAAG AGATGGAATC TGTTATAAAG 
GACATTAAAA ACACCACACA GAAGAAATAC ATGGATTATG GGAAGAACCC TGGGTCACCT 
GACAATGACT TCCTCTTCAT GTACTCCGTG GCCAGAACAA ATCTGGAGCT TGAGTTGGTT 
CATCGTGGTG GGAATCTATG CAGTGGAGGG GCCAGTGCAG CTGCCAAACG CTCCTGTCTC 
AATCAGCTGT TTCACGTGCT GGCCATGCAC ATGCGGCTCT ACAGCATAGA CTCGGCATAC 
AATCCATGGA CCAGACTCAC ACTGAGCACG CAAAGCAGAG AGAATGAGTA CTGTGATGAA 
GAGCGTCCTG AGGTTCCCAT GCTTTTCCGA GACGTCCCAT CCCTCCTAAT CATCTTCATC 
CTCACCATGC CTCAGCCTTT ACGCAAAGAG CACTTCACGT GTGTGGTGAA GGTGTTGTAC 
AGTCTGCAGT TCACACAGGC TCTGGCTGCG CTCTCCATCA GGTTCAGCCG TGAGGAGAGA 
CTCGCCTGGA GCAACACTGG AGCTGCCAAG AAGAACCTGC CAAACTCTGA CAAATCATGG 
GAGTCCCTCC TGGGTCACAT GATCTCCGAG CTGACGAAGG CCAAAGACGT GTATGACACC 
AACTCTGAGG AAACATCCGC TTTGAGCTCC AGTGTTTGGT CTCCTCAGTC CATTGAGTTC 
AGGCTCCAGC AGTTCTGTCT GCCCTTCCTG AGACTCTCCT GTTTACTGCA GCACCACCTG 
TATGGAGACA GCTTACCCGG CTGCCTGGTG GAGGAGGAGT TCTCTCTGCT GACGCGCTGT 
CTGGGTTTAG CTGCTTCTGT TCAGTGCAGT GGATCCATGA GCAGCGCAGC GTGTCTGGAG 
TGGAACATCA GTGCTTTTGA CTTGATCAGT CAGTGGTGCT CAGAAGTCGT CGCTCTGTCC 
GACACGCCCT CCCAGCAGTC TGCGTCTCTG TTAGGTCAGG ACCCGCAGTG GGCTGCTCCT 
CGTCTCCTGC AGCTTCCCGA CAATTACAAC ACCATCTTCC AGTACTACCA CAGGAAATCC 
TGCAGCAGCT GCGGAAAGAC CCCCAAAGAC CCTGCGCTGT GCCTGGTGTG CGGCGCGTTC 
GTCTGCCTGA AGGGTCATTG CTGTAAGCAG CAGGGCGTCT GCGAGTGTGT GCTGCACTCC 
CAGCACTGCG GAGCGGCGAC TGGTATCTTC CTCCTGATAA ACGCATCCGT CATCATTATC 
ATCCGAGGGC ATCGCTTCTG CCTGTGGGGG TCTGTGTATC TGGATGCTCA CGGAGAGGAA 
GACCGAGACC TACGTCGAGG AAAGCCTCTG TATTTGTGCG AGGAGAGATA TCGTGTTCTG 
GAGCAGCAGT GGGTCGCTCA CACCTTCGAC CACATCAACA AGCGCTGGGG TCCTCATTAC 
AACGGACTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213227.1
CDS	78..5669
Translation	MMAASLLRRDKKSTAAHLKADLKRTDNSSGLRQLQELLDSVLNPERGSDPEALDWCKWLLAGGDGFDEFCRTVRSYDNATLCGLVWTANFVAYRCRTCGISPCMSLCAECFNNGDHTGHDFNMFRSQAGGACDCGDGNVMRESGFCNRHRLKTGENVPSVPRDLLLMSEMVLPRFIITIIQYLRDGYTEPESAADRDLQKVLQQLDPHISFLEELTKMGGAMRTVLTKILTDQQTFKELSMGQEDNVYAKKNYEKYLSALKSSGLVSVEEKGAAGGAGDGTSDAAAGAGALSLLGATATASLDDSSKEEDQDGLQGVGQRKRVKLSSSTKDPSIMDTLKHKCFLEELLFWTIKYEFPQKMVTFLLNMLPDQDYKITFTKTFVQHYAFIMKTLMKSHESDTMSNRIVHISVQLFSNEELARHVTEECQLLDIMVTVLLYMMESCLIKSELQDEENNRHVVVNCGEALLKNNTYWPLVSDFINILSHQSVAKRFLEDHSLLLLWMSFVSFFQGMNLNKRELNEHVEFESQTYYAAFAAELEACAQPMWGLLTHCKVKETQDYTKTVVRYCLETLQMWFDAIGFVDEPSLNQLTFHLPLHRYYAMFLSKGVKCQGLDLDSLLPDQEMLMKIMVHPLQIQASLSEIHSNMWVRNGLQIKGQAMTYVQSHFCNSMIDPDIYLLQVCASRLDPDYFISSVFERFKVVDLLTMASQHQNAVLDSEQERPMLEGALTFLVILCSLRIHLGMSDDEILRAEMVSQLCMNDRTHSSLLDLIPENPNPKSGVVPGSCSFEEMLSGVADFKAPVFEPGGSMQQGMYTPKAEVWEKEFDPIMVILRTVYRRDVQSAMDRYSAFLKQSGVHTGNPWPPYKERTPLHPCYKGLVRLLHCKTLHIVIFTLLYKIWMDHQNMSEHVLCMVLYLIELGLDNQVQDDKVEEEPCIEEHCHDSWFPGTSLLSNLHHVINFVRVRVPETAPEVERKRERERERETPPSTSSESATFGQNLREAQVFSLVAERRRKFQEIINRSNHEASQAVRPKSSASRWLPPGTPPQLVTEILEIRESMLSLLVKLHQKLSAKQNSLSLSWLAEVDPAHHAHGDGLTAIERILAKASARSRHSKRCLQEICGKVCPPIPPKKISPGDKKSMDKEERRQRARERQQKLLAEFASRQKSFMETAMDVESPEADAVMDVSSEESLDSEVLYDCVICGQSGPSTEDRPTGLVVLLQASSVLGHRCRSDMPKRLPTTDEEHIYPEDTCGATHDVRLSLMQRYFKDSSCLQSVSIGWDGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGISVDKGEFTCPLCRQFANSVLPCRPGRGMETGAWHAPSTKSMSTLVKEVEDLQEQLGIFPVRASIKQRDPILIESNLSKEMESVIKDIKNTTQKKYMDYGKNPGSPDNDFLFMYSVARTNLELELVHRGGNLCSGGASAAAKRSCLNQLFHVLAMHMRLYSIDSAYNPWTRLTLSTQSRENEYCDEERPEVPMLFRDVPSLLIIFILTMPQPLRKEHFTCVVKVLYSLQFTQALAALSIRFSREERLAWSNTGAAKKNLPNSDKSWESLLGHMISELTKAKDVYDTNSEETSALSSSVWSPQSIEFRLQQFCLPFLRLSCLLQHHLYGDSLPGCLVEEEFSLLTRCLGLAASVQCSGSMSSAACLEWNISAFDLISQWCSEVVALSDTPSQQSASLLGQDPQWAAPRLLQLPDNYNTIFQYYHRKSCSSCGKTPKDPALCLVCGAFVCLKGHCCKQQGVCECVLHSQHCGAATGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYLCEERYRVLEQQWVAHTFDHINKRWGPHYNGL

Target ORF information:

RefSeq Version	XM_017357738.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3 component n-recognin 3 (ubr3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357738.1

ORF Insert Sequence:

ATGATGGCGG CGTCTCTGCT GCGCCGGGAC AAGAAATCCA CCGCCGCCCA TCTGAAGGCA 
GACCTCAAGC GGACTGACAA CAGTTCAGGC TTGCGGCAGC TGCAGGAACT GCTGGATAGC 
GTACTGAACC CGGAGCGGGG CTCGGACCCG GAGGCGCTGG ACTGGTGTAA ATGGCTGCTG 
GCCGGAGGGG ACGGTTTCGA CGAGTTCTGC CGGACCGTCC GATCCTACGA TAACGCCACC 
CTCTGCGGGC TGGTGTGGAC TGCCAACTTC GTGGCCTACC GTTGCCGGAC TTGCGGCATT 
TCCCCGTGCA TGTCGTTATG CGCGGAGTGC TTCAATAACG GCGACCACAC GGGCCATGAT 
TTTAACATGT TCCGGAGTCA GGCCGGCGGG GCCTGTGACT GCGGGGACGG GAACGTGATG 
AGAGAGAGCG GGTTTTGTAA CAGACATCGA TTAAAAACAG GAGAGAACGT TCCATCAGTC 
CCTCGAGATC TGCTGCTCAT GTCTGAAATG GTGCTGCCGC GGTTTATCAT CACTATCATT 
CAATATCTGA GAGACGGCTA CACTGAACCA GAGTCGGCCG CTGACAGAGA TCTTCAGAAG 
GTTCTGCAGC AGTTGGATCC ACACATCTCC TTCTTAGAAG AGCTCACCAA GATGGGCGGA 
GCCATGCGCA CCGTTCTCAC CAAGATCTTA ACCGATCAGC AGACCTTTAA AGAGCTCAGC 
ATGGGTCAAG AGGATAATGT TTATGCAAAG AAGAACTATG AGAAGTATTT GTCGGCATTG 
AAGAGCTCAG GTCTGGTGTC AGTGGAGGAG AAAGGAGCCG CTGGAGGAGC TGGAGACGGG 
ACATCAGATG CTGCAGCAGG GGCAGGAGCG CTCAGTCTAC TGGGAGCCAC TGCTACTGCT 
AGTCTGGATG ACTCCAGCAA AGAGGAGGAT CAGGATGGGC TGCAGGGTGT GGGTCAGAGG 
AAAAGAGTGA AGCTCAGTAG CAGTACCAAA GATCCATCCA TAATGGACAC GCTGAAACAT 
AAATGCTTTT TAGAAGAGTT ACTGTTTTGG ACTATTAAGT ATGAATTTCC ACAGAAGATG 
GTGACGTTTT TGCTCAACAT GTTGCCAGAT CAGGATTACA AGATCACCTT CACTAAAACA 
TTCGTACAAC ACTATGCCTT CATCATGAAG ACCCTCATGA AGAGTCACGA GTCAGACACC 
ATGTCCAACC GCATCGTGCA CATCAGCGTG CAGCTCTTCA GCAACGAGGA GCTGGCCAGA 
CACGTCACAG AGGAGTGCCA GCTACTGGAC ATCATGGTCA CCGTTCTCCT CTACATGATG 
GAGAGCTGCC TTATCAAGAG CGAACTGCAA GATGAGGAGA ATAATCGTCA TGTGGTGGTG 
AACTGTGGCG AAGCTCTGCT GAAGAATAAC ACCTACTGGC CTTTAGTTAG TGATTTTATT 
AATATCCTAT CACACCAAAG TGTGGCCAAG CGCTTCCTGG AGGATCATTC TCTGCTGCTG 
CTCTGGATGA GTTTTGTCTC CTTCTTTCAA GGAATGAATC TGAATAAGCG GGAGCTGAAT 
GAGCACGTGG AGTTTGAGTC TCAGACATAT TACGCAGCGT TTGCTGCTGA ACTGGAGGCC 
TGCGCTCAGC CCATGTGGGG TCTCCTCACA CACTGCAAAG TTAAAGAGAC CCAAGACTAC 
ACTAAGACAG TGGTGCGGTA CTGTCTGGAA ACTCTTCAGA TGTGGTTTGA TGCCATTGGG 
TTTGTAGATG AGCCTTCCCT CAACCAGCTG ACGTTTCATT TACCTCTTCA CCGATACTAC 
GCCATGTTCC TCAGCAAGGG AGTGAAGTGT CAAGGTCTGG ATTTAGACAG TTTGCTTCCT 
GATCAGGAGA TGCTGATGAA GATCATGGTT CACCCCCTAC AGATTCAGGC TAGCCTGTCT 
GAGATCCATA GTAACATGTG GGTGAGAAAT GGGCTGCAGA TTAAAGGTCA GGCCATGACC 
TATGTCCAGT CTCACTTCTG CAACTCCATG ATTGATCCGG ACATCTACCT CCTGCAGGTG 
TGCGCTTCTA GACTGGATCC AGATTACTTC ATTTCCTCAG TCTTCGAGAG GTTCAAGGTG 
GTGGATCTGC TGACGATGGC GTCTCAACAT CAGAACGCAG TGCTGGACTC TGAACAAGAG 
AGGCCCATGC TGGAGGGGGC GCTCACCTTC TTGGTCATAC TGTGCAGTCT CAGGATACAC 
TTAGGAATGT CTGACGATGA GATTTTGAGA GCCGAGATGG TGTCACAGTT GTGTATGAAT 
GATCGTACCC ACAGCTCTCT GCTGGATCTT ATCCCTGAGA ACCCGAACCC TAAGAGCGGT 
GTGGTGCCGG GCAGCTGTAG TTTTGAGGAG ATGCTGTCTG GTGTAGCGGA TTTTAAAGCC 
CCTGTGTTTG AGCCTGGAGG ATCCATGCAG CAGGGCATGT ACACACCTAA AGCGGAGGTT 
TGGGAAAAAG AGTTTGACCC TATAATGGTG ATTCTACGCA CCGTTTACCG CAGAGACGTG 
CAGTCGGCCA TGGATCGATA CTCAGCATTT TTGAAACAGT CAGGCGTTCA CACAGGGAAC 
CCTTGGCCAC CCTATAAGGA GAGGACTCCT TTGCATCCCT GCTATAAAGG ACTGGTGCGC 
CTGCTGCACT GCAAAACCTT ACATATCGTC ATCTTTACAC TGCTTTATAA GATATGGATG 
GATCATCAGA ACATGTCTGA GCATGTGTTG TGTATGGTGC TGTACCTGAT AGAGCTTGGA 
CTTGATAATC AGGTCCAGGA TGACAAAGTG GAAGAGGAGC CCTGTATCGA GGAGCACTGC 
CACGACAGCT GGTTCCCGGG CACCAGCCTG CTGTCTAACC TCCATCACGT CATCAACTTT 
GTGCGGGTTC GAGTGCCGGA GACGGCTCCG GAGGTGGAGA GGAAGCGAGA GAGGGAACGA 
GAGAGAGAAA CTCCACCCAG CACCAGTTCA GAGAGCGCCA CCTTTGGTCA GAATCTGCGT 
GAAGCTCAGG TGTTCAGTCT GGTGGCGGAG CGCAGGAGAA AGTTCCAGGA GATCATCAAC 
CGCAGCAACC ATGAGGCCAG TCAGGCAGTG CGCCCCAAGT CCTCAGCGTC CCGCTGGCTT 
CCACCGGGAA CGCCTCCACA GCTGGTGACC GAGATCCTGG AGATACGCGA GAGCATGCTG 
TCGCTGCTGG TTAAACTGCA CCAGAAACTG TCGGCCAAGC AGAACTCTCT GTCTCTGAGC 
TGGTTGGCCG AGGTGGATCC TGCCCATCAT GCGCACGGAG ACGGTTTAAC GGCCATCGAA 
AGAATCTTAG CCAAAGCTTC AGCTCGAAGC AGACACAGTA AAAGATGCCT ACAGGAGATC 
TGCGGGAAAG TGTGTCCACC CATACCGCCA AAGAAGATCA GTCCTGGAGA TAAGAAGAGC 
ATGGACAAGG AGGAGAGGCG TCAGCGCGCC AGAGAGAGGC AGCAGAAGCT GCTGGCGGAG 
TTTGCCTCCA GGCAGAAGAG CTTCATGGAG ACTGCCATGG ATGTTGAGTC CCCTGAAGCA 
GATGCTGTAA TGGACGTCAG CTCTGAGGAA TCTTTGGACT CTGAGGTTCT CTATGACTGT 
GTGATCTGTG GTCAGAGTGG ACCGTCCACT GAAGACAGAC CCACAGGACT GGTGGTTCTG 
CTGCAGGCTT CGTCAGTTTT GGGTCACCGT TGTCGTAGTG ACATGCCAAA GCGATTGCCC 
ACCACAGACG AAGAACACAT CTACCCTGAG GACACATGTG GAGCCACACA CGATGTCAGG 
CTGTCCCTTA TGCAACGCTA TTTTAAAGAT AGTTCCTGTC TGCAGTCTGT GTCTATTGGC 
TGGGACGGAG GGGTGTATGT GCAGACGTGT GGCCACACTC TGCATATAGA CTGTCACAAG 
TCCTACATGG AGTCCTTACG GAATGATCAG GTTCTTCAGG GGATTTCTGT GGATAAAGGC 
GAGTTCACTT GTCCGCTTTG CCGCCAGTTT GCAAACAGTG TTTTACCTTG TCGTCCTGGA 
CGAGGGATGG AAACCGGAGC CTGGCACGCA CCCAGTACCA AGAGCATGTC TACACTGGTG 
AAAGAAGTAG AAGACCTCCA GGAGCAGCTG GGCATTTTCC CAGTAAGAGC CAGCATAAAG 
CAAAGAGATC CCATTCTGAT CGAGTCAAAC CTGAGTAAAG AGATGGAATC TGTTATAAAG 
GACATTAAAA ACACCACACA GAAGAAATAC ATGGATTATG GGAAGAACCC TGGGTCACCT 
GACAATGACT TCCTCTTCAT GTACTCCGTG GCCAGAACAA ATCTGGAGCT TGAGTTGGTT 
CATCGTGGTG GGAATCTATG CAGTGGAGGG GCCAGTGCAG CTGCCAAACG CTCCTGTCTC 
AATCAGCTGT TTCACGTGCT GGCCATGCAC ATGCGGCTCT ACAGCATAGA CTCGGCATAC 
AATCCATGGA CCAGACTCAC ACTGAGCACG CAAAGCAGAG AGAATGAGTA CTGTGATGAA 
GAGCGTCCTG AGGTTCCCAT GCTTTTCCGA GACGTCCCAT CCCTCCTAAT CATCTTCATC 
CTCACCATGC CTCAGCCTTT ACGCAAAGAG CACTTCACGT GTGTGGTGAA GGTGTTGTAC 
AGTCTGCAGT TCACACAGGC TCTGGCTGCG CTCTCCATCA GGTTCAGCCG TGAGGAGAGA 
CTCGCCTGGA GCAACACTGG AGCTGCCAAG AAGAACCTGC CAAACTCTGA CAAATCATGG 
GAGTCCCTCC TGGGTCACAT GATCTCCGAG CTGACGAAGG CCAAAGACGT GTATGACACC 
AACTCTGAGG AAACATCCGC TTTGAGCTCC AGTGTTTGGT CTCCTCAGTC CATTGAGTTC 
AGGCTCCAGC AGTTCTGTCT GCCCTTCCTG AGACTCTCCT GTTTACTGCA GCACCACCTG 
TATGGAGACA GCTTACCCGG CTGCCTGGTG GAGGAGGAGT TCTCTCTGCT GACGCGCTGT 
CTGGGTTTAG CTGCTTCTGT TCAGTGCAGT GGATCCATGA GCAGCGCAGC GTGTCTGGAG 
TGGAACATCA GTGCTTTTGA CTTGATCAGT CAGTGGTGCT CAGAAGTCGT CGCTCTGTCC 
GACACGCCCT CCCAGCAGTC TGCGTCTCTG TTAGGTCAGG ACCCGCAGTG GGCTGCTCCT 
CGTCTCCTGC AGCTTCCCGA CAATTACAAC ACCATCTTCC AGTACTACCA CAGGAAATCC 
TGCAGCAGCT GCGGAAAGAC CCCCAAAGAC CCTGCGCTGT GCCTGGTGTG CGGCGCGTTC 
GTCTGCCTGA AGGGTCATTG CTGTAAGCAG CAGGGCGTCT GCGAGTGTGT GCTGCACTCC 
CAGCACTGCG GAGCGGCGAC TGGTATCTTC CTCCTGATAA ACGCATCCGT CATCATTATC 
ATCCGAGGGC ATCGCTTCTG CCTGTGGGGG TCTGTGTATC TGGATGCTCA CGGAGAGGAA 
GACCGAGACC TACGTCGAGG AAAGCCTCTG TATTTGTGCG AGGAGAGATA TCGTGTTCTG 
GAGCAGCAGT GGGTCGCTCA CACCTTCGAC CACATCAACA AGCGCTGGGG TCCTCATTAC 
AACGGACTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ubr3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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