dec-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dec-1
Entrez Gene ID	31691
Full Name	defective chorion 1
Synonyms	39051,CG2175,Dmel\CG2175,Fc,Fc1,Fcp7C,dec,dec1,dec[[1]],fs(1)11-549,fs(1)12-1873,fs(1)12-2514,fs(1)12-3512,fs(1)12-365,fs(1)12-3907,fs(1)12-403,fs(1)12-4860,fs(1)13-453,fs(1)13C-57,fs(1)13C-73,fs(1)14-963,fs(1)14A-114,fs(1)14E19,fs(1)1501,fs(1)384,fs(1)5,fs(1)A257,fs(1)A384,fs(1)C1,fs(1)M102,fs(1)M1021
General protein information	Preferred Names defective chorion 1 Names CG2175 gene product from transcript CG2175-RG CG2175-PA CG2175-PB CG2175-PC CG2175-PE CG2175-PF CG2175-PG dec-1-PA dec-1-PB dec-1-PC dec-1-PE dec-1-PF dec-1-PG defective chorion follicle cell protein 7C
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 7C1-7C1\|1-20.7 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_167133.2	NP_727203.1	defective chorion 1, isoform C
NM_078517.3	NP_511072.2	defective chorion 1, isoform A
NM_001258641.1	NP_001245570.1	defective chorion 1, isoform E
NM_001258642.1	NP_001245571.1	defective chorion 1, isoform F
NM_001258640.2	NP_001245569.1	defective chorion 1, isoform G
NM_167132.2	NP_727202.1	defective chorion 1, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dominant female sterility was not associated with larger deletions that included the fc106 N terminus, suggesting that abnormal juxtaposition of N- and C-terminal sequences in the aberrant proprotein interfered with endogenous DEC-1 proteins.
Title: A mutant dec-1 transgene induces dominant female sterility in Drosophila melanogaster.

Capsulin shared sequence homology with eggshell proteins encoded by the Drosophila dec-1 gene.
Title: Aplysia capsulin is localized to egg capsules and egg cordon sheaths and shares sequence homology with Drosophila dec-1 gene products.

Expression of a fc177 cDNA in dec-1 null mutants was sufficient to restore spaces within the endochorion layer. Fc177 may function as a scaffolding protein akin to those utilized in viral morphogenesis.
Title: fc177, a minor dec-1 proprotein, is necessary to prevent ectopic aggregation of the endochorion during eggshell assembly in Drosophila.

The following dec-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dec-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01037	NM_167132.2 Latest version!	Drosophila melanogaster defective chorion 1 (dec-1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa01038	NM_078517.3 Latest version!	Drosophila melanogaster defective chorion 1 (dec-1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa01039	NM_167133.2 Latest version!	Drosophila melanogaster defective chorion 1 (dec-1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa03016	NM_001258641.1 Latest version!	Drosophila melanogaster defective chorion 1 (dec-1), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa03017	NM_001258640.2 Latest version!	Drosophila melanogaster defective chorion 1 (dec-1), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa03018	NM_001258642.1 Latest version!	Drosophila melanogaster defective chorion 1 (dec-1), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dec-1 ( NM_167132.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727202.1 Drosophila melanogaster defective chorion 1 (dec-1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01037
Clone ID Related Accession (Same CDS sequence)	NM_167132.2
Accession Version	NM_167132.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	defective chorion 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On May 8, 2012 this sequence version replaced NM_167132.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGATTGT TTAGCCTTCT GCCGCTCCTG GCGCTTCTTG TCGTCCAGGC TGCCGGACAA 
AGTGAAGTAA CCTCTGATGA TCCTGCAACG GATGCTGGAT CAACGACCAA TTCCACCACG 
GACACCAAAC CAAGGATTCC CAGTCAGGAT GAGATCCTTG GCCAGATGCC GTCCATTAAT 
CCCATCCGCA CTGGCAATCC CCAGATGGAC GCATTTTACA TGATGTTCCC GGCGTTGGGC 
AGCCTGCTCA AGTGGGGCAG CCTTTTCCCA GCCTACTCGA TTCTGGGCGC CATTCCCGAC 
AATCTACAGC CTACAGCGGC AGCCTCCAAG GTGGTCCTTG TCCTGGCCGA TGATGCGACG 
GCCAAGACTC GAGTTGCCCG TCAAAATCCG CCACCAAATC CACTTGGTCA GCTAATGAAT 
TGGCCCGCTC TGCCGCAGGA CTTCCAACTG CCTTCCATGG ATCTGGGACC GCAAGTGGGC 
TCGTTTTTGG CCCAACTGCC TGCTATGCCT ACCGTGCCTG GTCTTCTGGG TGCCGCTGCT 
CCAGTTCCTG CTCCAGCTCC TGCTCCCGCT GCTGCTCCTC CTCCGGCGCC AGCTCCAGCT 
GCAGATCCTC CGGCAGCACC TGTTCCAGAT GCACCCCAAC CAGCCATACT GGGACAAGCC 
GCTCTGCAGA ACGCTTTCAC CTTCTTTAAC CCGGCTAACT TTGATGCCTC CAGTCTTCTG 
GGCCAGAGTG TACCCACATT TGCTCCTCCA AATCTTGATT TCGTCGCTCA AATGCAAAGG 
CAATTCTTCC CAGGAATGAC ACCGGCACAA CCTGCTGCCG CTGGCACGGA TGCCCAGGCC 
TCCGACATTT CCGAGGTAAG GGTACGTCCT GAGGATCCGT ATTCGCAAGA GGCACAGATG 
AAGATCAAAT CGGCACTTGA AATGGAGCAG GAGAGGCAAC AACAGGCTCA GGTCAAGGAT 
CAGGAGCAAG TGCCTCTCCT CTGGTTCCGA ATGCCCACAA CTCAGAATCA GGATGCGACT 
GAAGAAAAGA CTCTGGAGGA TCTGCGGGTC GAGGCCAAAT TGAGGGCATT CGAGCGCCAG 
GTGATAGCCG AATTGAGAAT GCTGCAGAAG ATCGAACTTA TGGCTAAGCA GATGAGGTCC 
AGCGCCGCAG CTCAAAACGG AGATTCACCC TACAGAATCA GTTATCCACT GAGTCGGACA 
CCCATTCACA AGATCACCCG TGCTGATATT GAGCAAGCCC TTCGGGATGA TTATGTCCGC 
CGATTGGTCA ACAAGGAAGC GCAACGAAGG GCTAGAAATT CCGGCATAAA CACCCAGAAG 
GCAAATGCCT TGAAGCGACA GGCCAAATCC CAGGATCAGA CTCTGTCCAA GGAGGATATC 
GTCCAGATTA TGGCGTATGC CTATCGCATG GCCAACGAAC AGATGGAGAG CGAGAAGGGC 
AAGCAGGACA AGGTTTACGC GGCCTACAGG ACGGAACAGA ATCCAATGAT GATGCAGCAA 
AGACAATGGT CGGAGGAGCA GGCCAAGATC CAACAGAATC AACAGCAGAT CCAGCAAAAT 
CCAATGATGA TGCAGCAACG ACAATGGTCG GAGGAGCAGG CCAAGATCCA ACAGAATCAA 
CAGCAGATCC AGCAAAATCC AATGATGATG CAGCAACGAC AATGGTCGGA GGAGCAGGCC 
AAGATCCAAC AGAATCAACA GCAGATCCAG CAAAATCCAA TGATGATGCA GCAAAGACAA 
TGGTCGGAGG AGCAGGCTAA GATCCAACAG AATCAACAGC AGATCCAGCA AAATCCAATG 
ATGGTGCAGC AAAGACAATG GTCGGAGGAG CAGGCCAAGA TCCAACAGAA TCAACAGCAG 
ATCCAGCAAA ATCCAATGAT GATGCAGCAA AGACAATGGT CGGAGGAGCA GGCCAAGATT 
CAGCATGATC AACAGATGGC ACAACAGATG GCACAGCAAG GTCTCATGAT GACGGAGCAG 
AGGCAAAGGC AGTGGTCAGA AGATCAGGCC AAAATTCAGC AGGCTCAACA GATGGCCCAA 
CAGACACCCA TGATGATGCC ACAGATGCAA CAAAGGCAGT GGACAGAGGA TCCCCAAATG 
GTTCAGCAGA TGCAACAGAG GCAGTGGGCA GAAGATCAGA CCAGGATGCA AATGGCCCAA 
CAGAATCCAA TGATGCAACA GCAGCGACAG ATGGCAGAGA ATCCGCAAAT GATGCAGCAA 
AGGCAATGGT CCGAGGAGCA GACCAAAATC GAACAGGCTC AGCAGATGGC ACAACAGAAT 
CAAATGATGA TGCAGCAGAT GCAGCAAAGA CAATGGTCGG AGGATCAGGC CCAGATTCAA 
CAGCAGCAGA GACAGATGAT GCAGCAGACC CCCATGATGA TGAAGGAGCG CCAGTGGGCG 
GAGGAGAACC CCCAGTCTGT CCAGCAGCAA GGGCCCATGA TGATGCAGCA GCAGATGCCA 
TCGATGATGC AGCGTGAAGT AGAGGACGAA GATAATAAGG CAGAGGATGA CTTAGTTGGC 
GAGGCGGGAC CCCAAATGCC GGAGAACGAA GGCACCGCCA GGCACAAAGT CGATGCCCTG 
GGAGTTGGCG GCAACAAGCG CAAGAAGTCC AAGTCCAAGT CGGCGCCGCC AACGGTAATT 
AACTATTACT ATGCGGCACC CCAGCGTCCG GTGGTTCAGA GTTACGGAAC AAGCTACGGC 
GGAGGTGGCT ATGGATCGAA TGCCTATGGT GTTCCTCGTC CAGTCAATTC GTATCAAAGT 
CAAGGCTACA GGGCTGCCGT GGGTAACGAT GAGGTAGATG AGATGTTGCG CCAGCACCAG 
ACAATGGCCA GGACAATAAA CCCGAAGCAA CCAGGCGAAG TCGGTGGATC GGAGAGCCAG 
AAGAGCAACT CTAATCCTCC AACGACGTTG ACACCAGCTC CACAAGAGCA ACCGCAAGAG 
CATCGAGTCC ACAAAAGTCC ATCATCAGCA CCATCCGAAA CCGAAATCGA AAACGCACCA 
TCATCTGACC CCCAAGTGGG TTCCATTTTC ACCTACGGCG AGGGATTGCT GCATCCGTTT 
ATGGGTCTGC TGCCGGTGGA GAGGCCCGAT GATCCGTGGA ACCAGAAGCC ATACGATCCT 
CACCATCCTC TCTACACGGG CGGTGGGAGC TACGATGCCT ATCTGAGGGA CGGTCGCCAT 
CGAAGGGACA CGCACATCAT GGGTCAAGGT ACGCAGCATG GCATCCTGAC ACCCGGCATG 
TTGGAGAGAC TTCTTCGGAT CAAGATGGAT TTCCAGCGTA GATTTCCCCA TTTGTACAAG 
GGTATGCTGA ATCACCATAC GAATCTTACC CGTGTAGAGG TACAGCCTCC GGTTCTGGGC 
AAGATTTCTA AGCCGAAAAC TAAGACCAAG CCCAAGAATG AGGACGAACC CGTCTTCGAA 
TTGGGAGCCG CTGAGCGTAG CCTGTTCGAG GACGAGACCA ACGATTCTTT AGAGAAAGAT 
CCCGAACCAG AACCGGATGA GGAGGATGAC CGGGATGTGG AAGAGCCCAG CGAGAGCAGT 
GAACCTAGGG GATTCAGCAG CAAAAAAAGC CGGGATGAGA ACGACATCGA TTACTTCAAT 
TTCGATGACG ATGATGTTGA TGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727202.1
CDS	76..3702
Translation	MRLFSLLPLLALLVVQAAGQSEVTSDDPATDAGSTTNSTTDTKPRIPSQDEILGQMPSINPIRTGNPQMDAFYMMFPALGSLLKWGSLFPAYSILGAIPDNLQPTAAASKVVLVLADDATAKTRVARQNPPPNPLGQLMNWPALPQDFQLPSMDLGPQVGSFLAQLPAMPTVPGLLGAAAPVPAPAPAPAAAPPPAPAPAADPPAAPVPDAPQPAILGQAALQNAFTFFNPANFDASSLLGQSVPTFAPPNLDFVAQMQRQFFPGMTPAQPAAAGTDAQASDISEVRVRPEDPYSQEAQMKIKSALEMEQERQQQAQVKDQEQVPLLWFRMPTTQNQDATEEKTLEDLRVEAKLRAFERQVIAELRMLQKIELMAKQMRSSAAAQNGDSPYRISYPLSRTPIHKITRADIEQALRDDYVRRLVNKEAQRRARNSGINTQKANALKRQAKSQDQTLSKEDIVQIMAYAYRMANEQMESEKGKQDKVYAAYRTEQNPMMMQQRQWSEEQAKIQQNQQQIQQNPMMMQQRQWSEEQAKIQQNQQQIQQNPMMMQQRQWSEEQAKIQQNQQQIQQNPMMMQQRQWSEEQAKIQQNQQQIQQNPMMVQQRQWSEEQAKIQQNQQQIQQNPMMMQQRQWSEEQAKIQHDQQMAQQMAQQGLMMTEQRQRQWSEDQAKIQQAQQMAQQTPMMMPQMQQRQWTEDPQMVQQMQQRQWAEDQTRMQMAQQNPMMQQQRQMAENPQMMQQRQWSEEQTKIEQAQQMAQQNQMMMQQMQQRQWSEDQAQIQQQQRQMMQQTPMMMKERQWAEENPQSVQQQGPMMMQQQMPSMMQREVEDEDNKAEDDLVGEAGPQMPENEGTARHKVDALGVGGNKRKKSKSKSAPPTVINYYYAAPQRPVVQSYGTSYGGGGYGSNAYGVPRPVNSYQSQGYRAAVGNDEVDEMLRQHQTMARTINPKQPGEVGGSESQKSNSNPPTTLTPAPQEQPQEHRVHKSPSSAPSETEIENAPSSDPQVGSIFTYGEGLLHPFMGLLPVERPDDPWNQKPYDPHHPLYTGGGSYDAYLRDGRHRRDTHIMGQGTQHGILTPGMLERLLRIKMDFQRRFPHLYKGMLNHHTNLTRVEVQPPVLGKISKPKTKTKPKNEDEPVFELGAAERSLFEDETNDSLEKDPEPEPDEEDDRDVEEPSESSEPRGFSSKKSRDENDIDYFNFDDDDVDD

Target ORF information:

RefSeq Version	NM_167132.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster defective chorion 1 (dec-1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167132.2

ORF Insert Sequence:

ATGAGATTGT TTAGCCTTCT GCCGCTCCTG GCGCTTCTTG TCGTCCAGGC TGCCGGACAA 
AGTGAAGTAA CCTCTGATGA TCCTGCAACG GATGCTGGAT CAACGACCAA TTCCACCACG 
GACACCAAAC CAAGGATTCC CAGTCAGGAT GAGATCCTTG GCCAGATGCC GTCCATTAAT 
CCCATCCGCA CTGGCAATCC CCAGATGGAC GCATTTTACA TGATGTTCCC GGCGTTGGGC 
AGCCTGCTCA AGTGGGGCAG CCTTTTCCCA GCCTACTCGA TTCTGGGCGC CATTCCCGAC 
AATCTACAGC CTACAGCGGC AGCCTCCAAG GTGGTCCTTG TCCTGGCCGA TGATGCGACG 
GCCAAGACTC GAGTTGCCCG TCAAAATCCG CCACCAAATC CACTTGGTCA GCTAATGAAT 
TGGCCCGCTC TGCCGCAGGA CTTCCAACTG CCTTCCATGG ATCTGGGACC GCAAGTGGGC 
TCGTTTTTGG CCCAACTGCC TGCTATGCCT ACCGTGCCTG GTCTTCTGGG TGCCGCTGCT 
CCAGTTCCTG CTCCAGCTCC TGCTCCCGCT GCTGCTCCTC CTCCGGCGCC AGCTCCAGCT 
GCAGATCCTC CGGCAGCACC TGTTCCAGAT GCACCCCAAC CAGCCATACT GGGACAAGCC 
GCTCTGCAGA ACGCTTTCAC CTTCTTTAAC CCGGCTAACT TTGATGCCTC CAGTCTTCTG 
GGCCAGAGTG TACCCACATT TGCTCCTCCA AATCTTGATT TCGTCGCTCA AATGCAAAGG 
CAATTCTTCC CAGGAATGAC ACCGGCACAA CCTGCTGCCG CTGGCACGGA TGCCCAGGCC 
TCCGACATTT CCGAGGTAAG GGTACGTCCT GAGGATCCGT ATTCGCAAGA GGCACAGATG 
AAGATCAAAT CGGCACTTGA AATGGAGCAG GAGAGGCAAC AACAGGCTCA GGTCAAGGAT 
CAGGAGCAAG TGCCTCTCCT CTGGTTCCGA ATGCCCACAA CTCAGAATCA GGATGCGACT 
GAAGAAAAGA CTCTGGAGGA TCTGCGGGTC GAGGCCAAAT TGAGGGCATT CGAGCGCCAG 
GTGATAGCCG AATTGAGAAT GCTGCAGAAG ATCGAACTTA TGGCTAAGCA GATGAGGTCC 
AGCGCCGCAG CTCAAAACGG AGATTCACCC TACAGAATCA GTTATCCACT GAGTCGGACA 
CCCATTCACA AGATCACCCG TGCTGATATT GAGCAAGCCC TTCGGGATGA TTATGTCCGC 
CGATTGGTCA ACAAGGAAGC GCAACGAAGG GCTAGAAATT CCGGCATAAA CACCCAGAAG 
GCAAATGCCT TGAAGCGACA GGCCAAATCC CAGGATCAGA CTCTGTCCAA GGAGGATATC 
GTCCAGATTA TGGCGTATGC CTATCGCATG GCCAACGAAC AGATGGAGAG CGAGAAGGGC 
AAGCAGGACA AGGTTTACGC GGCCTACAGG ACGGAACAGA ATCCAATGAT GATGCAGCAA 
AGACAATGGT CGGAGGAGCA GGCCAAGATC CAACAGAATC AACAGCAGAT CCAGCAAAAT 
CCAATGATGA TGCAGCAACG ACAATGGTCG GAGGAGCAGG CCAAGATCCA ACAGAATCAA 
CAGCAGATCC AGCAAAATCC AATGATGATG CAGCAACGAC AATGGTCGGA GGAGCAGGCC 
AAGATCCAAC AGAATCAACA GCAGATCCAG CAAAATCCAA TGATGATGCA GCAAAGACAA 
TGGTCGGAGG AGCAGGCTAA GATCCAACAG AATCAACAGC AGATCCAGCA AAATCCAATG 
ATGGTGCAGC AAAGACAATG GTCGGAGGAG CAGGCCAAGA TCCAACAGAA TCAACAGCAG 
ATCCAGCAAA ATCCAATGAT GATGCAGCAA AGACAATGGT CGGAGGAGCA GGCCAAGATT 
CAGCATGATC AACAGATGGC ACAACAGATG GCACAGCAAG GTCTCATGAT GACGGAGCAG 
AGGCAAAGGC AGTGGTCAGA AGATCAGGCC AAAATTCAGC AGGCTCAACA GATGGCCCAA 
CAGACACCCA TGATGATGCC ACAGATGCAA CAAAGGCAGT GGACAGAGGA TCCCCAAATG 
GTTCAGCAGA TGCAACAGAG GCAGTGGGCA GAAGATCAGA CCAGGATGCA AATGGCCCAA 
CAGAATCCAA TGATGCAACA GCAGCGACAG ATGGCAGAGA ATCCGCAAAT GATGCAGCAA 
AGGCAATGGT CCGAGGAGCA GACCAAAATC GAACAGGCTC AGCAGATGGC ACAACAGAAT 
CAAATGATGA TGCAGCAGAT GCAGCAAAGA CAATGGTCGG AGGATCAGGC CCAGATTCAA 
CAGCAGCAGA GACAGATGAT GCAGCAGACC CCCATGATGA TGAAGGAGCG CCAGTGGGCG 
GAGGAGAACC CCCAGTCTGT CCAGCAGCAA GGGCCCATGA TGATGCAGCA GCAGATGCCA 
TCGATGATGC AGCGTGAAGT AGAGGACGAA GATAATAAGG CAGAGGATGA CTTAGTTGGC 
GAGGCGGGAC CCCAAATGCC GGAGAACGAA GGCACCGCCA GGCACAAAGT CGATGCCCTG 
GGAGTTGGCG GCAACAAGCG CAAGAAGTCC AAGTCCAAGT CGGCGCCGCC AACGGTAATT 
AACTATTACT ATGCGGCACC CCAGCGTCCG GTGGTTCAGA GTTACGGAAC AAGCTACGGC 
GGAGGTGGCT ATGGATCGAA TGCCTATGGT GTTCCTCGTC CAGTCAATTC GTATCAAAGT 
CAAGGCTACA GGGCTGCCGT GGGTAACGAT GAGGTAGATG AGATGTTGCG CCAGCACCAG 
ACAATGGCCA GGACAATAAA CCCGAAGCAA CCAGGCGAAG TCGGTGGATC GGAGAGCCAG 
AAGAGCAACT CTAATCCTCC AACGACGTTG ACACCAGCTC CACAAGAGCA ACCGCAAGAG 
CATCGAGTCC ACAAAAGTCC ATCATCAGCA CCATCCGAAA CCGAAATCGA AAACGCACCA 
TCATCTGACC CCCAAGTGGG TTCCATTTTC ACCTACGGCG AGGGATTGCT GCATCCGTTT 
ATGGGTCTGC TGCCGGTGGA GAGGCCCGAT GATCCGTGGA ACCAGAAGCC ATACGATCCT 
CACCATCCTC TCTACACGGG CGGTGGGAGC TACGATGCCT ATCTGAGGGA CGGTCGCCAT 
CGAAGGGACA CGCACATCAT GGGTCAAGGT ACGCAGCATG GCATCCTGAC ACCCGGCATG 
TTGGAGAGAC TTCTTCGGAT CAAGATGGAT TTCCAGCGTA GATTTCCCCA TTTGTACAAG 
GGTATGCTGA ATCACCATAC GAATCTTACC CGTGTAGAGG TACAGCCTCC GGTTCTGGGC 
AAGATTTCTA AGCCGAAAAC TAAGACCAAG CCCAAGAATG AGGACGAACC CGTCTTCGAA 
TTGGGAGCCG CTGAGCGTAG CCTGTTCGAG GACGAGACCA ACGATTCTTT AGAGAAAGAT 
CCCGAACCAG AACCGGATGA GGAGGATGAC CGGGATGTGG AAGAGCCCAG CGAGAGCAGT 
GAACCTAGGG GATTCAGCAG CAAAAAAAGC CGGGATGAGA ACGACATCGA TTACTTCAAT 
TTCGATGACG ATGATGTTGA TGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dec-1 ( NM_078517.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511072.2 Drosophila melanogaster defective chorion 1 (dec-1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa01038
Clone ID Related Accession (Same CDS sequence)	NM_078517.3
Accession Version	NM_078517.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	defective chorion 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On May 8, 2012 this sequence version replaced NM_078517.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGATTGT TTAGCCTTCT GCCGCTCCTG GCGCTTCTTG TCGTCCAGGC TGCCGGACAA 
AGTGAAGTAA CCTCTGATGA TCCTGCAACG GATGCTGGAT CAACGACCAA TTCCACCACG 
GACACCAAAC CAAGGATTCC CAGTCAGGAT GAGATCCTTG GCCAGATGCC GTCCATTAAT 
CCCATCCGCA CTGGCAATCC CCAGATGGAC GCATTTTACA TGATGTTCCC GGCGTTGGGC 
AGCCTGCTCA AGTGGGGCAG CCTTTTCCCA GCCTACTCGA TTCTGGGCGC CATTCCCGAC 
AATCTACAGC CTACAGCGGC AGCCTCCAAG GTGGTCCTTG TCCTGGCCGA TGATGCGACG 
GCCAAGACTC GAGTTGCCCG TCAAAATCCG CCACCAAATC CACTTGGTCA GCTAATGAAT 
TGGCCCGCTC TGCCGCAGGA CTTCCAACTG CCTTCCATGG ATCTGGGACC GCAAGTGGGC 
TCGTTTTTGG CCCAACTGCC TGCTATGCCT ACCGTGCCTG GTCTTCTGGG TGCCGCTGCT 
CCAGTTCCTG CTCCAGCTCC TGCTCCCGCT GCTGCTCCTC CTCCGGCGCC AGCTCCAGCT 
GCAGATCCTC CGGCAGCACC TGTTCCAGAT GCACCCCAAC CAGCCATACT GGGACAAGCC 
GCTCTGCAGA ACGCTTTCAC CTTCTTTAAC CCGGCTAACT TTGATGCCTC CAGTCTTCTG 
GGCCAGAGTG TACCCACATT TGCTCCTCCA AATCTTGATT TCGTCGCTCA AATGCAAAGG 
CAATTCTTCC CAGGAATGAC ACCGGCACAA CCTGCTGCCG CTGGCACGGA TGCCCAGGCC 
TCCGACATTT CCGAGGTAAG GGTACGTCCT GAGGATCCGT ATTCGCAAGA GGCACAGATG 
AAGATCAAAT CGGCACTTGA AATGGAGCAG GAGAGGCAAC AACAGGCTCA GGTCAAGGAT 
CAGGAGCAAG TGCCTCTCCT CTGGTTCCGA ATGCCCACAA CTCAGAATCA GGATGCGACT 
GAAGAAAAGA CTCTGGAGGA TCTGCGGGTC GAGGCCAAAT TGAGGGCATT CGAGCGCCAG 
GTGATAGCCG AATTGAGAAT GCTGCAGAAG ATCGAACTTA TGGCTAAGCA GATGAGGTCC 
AGCGCCGCAG CTCAAAACGG AGATTCACCC TACAGAATCA GTTATCCACT GAGTCGGACA 
CCCATTCACA AGATCACCCG TGCTGATATT GAGCAAGCCC TTCGGGATGA TTATGTCCGC 
CGATTGGTCA ACAAGGAAGC GCAACGAAGG GCTAGAAATT CCGGCATAAA CACCCAGAAG 
GCAAATGCCT TGAAGCGACA GGCCAAATCC CAGGATCAGA CTCTGTCCAA GGAGGATATC 
GTCCAGATTA TGGCGTATGC CTATCGCATG GCCAACGAAC AGATGGAGAG CGAGAAGGGC 
AAGCAGGACA AGGTTTACGC GGCCTACAGG ACGGAACAGA ATCCAATGAT GATGCAGCAA 
AGACAATGGT CGGAGGAGCA GGCCAAGATC CAACAGAATC AACAGCAGAT CCAGCAAAAT 
CCAATGATGA TGCAGCAACG ACAATGGTCG GAGGAGCAGG CCAAGATCCA ACAGAATCAA 
CAGCAGATCC AGCAAAATCC AATGATGATG CAGCAACGAC AATGGTCGGA GGAGCAGGCC 
AAGATCCAAC AGAATCAACA GCAGATCCAG CAAAATCCAA TGATGATGCA GCAAAGACAA 
TGGTCGGAGG AGCAGGCTAA GATCCAACAG AATCAACAGC AGATCCAGCA AAATCCAATG 
ATGGTGCAGC AAAGACAATG GTCGGAGGAG CAGGCCAAGA TCCAACAGAA TCAACAGCAG 
ATCCAGCAAA ATCCAATGAT GATGCAGCAA AGACAATGGT CGGAGGAGCA GGCCAAGATT 
CAGCATGATC AACAGATGGC ACAACAGATG GCACAGCAAG GTCTCATGAT GACGGAGCAG 
AGGCAAAGGC AGTGGTCAGA AGATCAGGCC AAAATTCAGC AGGCTCAACA GATGGCCCAA 
CAGACACCCA TGATGATGCC ACAGATGCAA CAAAGGCAGT GGACAGAGGA TCCCCAAATG 
GTTCAGCAGA TGCAACAGAG GCAGTGGGCA GAAGATCAGA CCAGGATGCA AATGGCCCAA 
CAGAATCCAA TGATGCAACA GCAGCGACAG ATGGCAGAGA ATCCGCAAAT GATGCAGCAA 
AGGCAATGGT CCGAGGAGCA GACCAAAATC GAACAGGCTC AGCAGATGGC ACAACAGAAT 
CAAATGATGA TGCAGCAGAT GCAGCAAAGA CAATGGTCGG AGGATCAGGC CCAGATTCAA 
CAGCAGCAGA GACAGATGAT GCAGCAGACC CCCATGATGA TGAAGGAGCG CCAGTGGGCG 
GAGGAGAACC CCCAGTCTGT CCAGCAGCAA GGGCCCATGA TGATGCAGCA GCAGATGCCA 
TCGATGATGC AGCGTGAAGT AGAGGACGAA GATAATAAGG CAGAGGATGA CTTAGTTGGC 
GAGGCGGGAC CCCAAATGCC GGAGAACGAA GGCACCGCCA GGCACAAAGT CGATGCCCTG 
GGAGTTGGCG GCAACAAGCG CAAGAAGTCC AAGTCCAAGT CGGCGCCGCC AACGGTAATT 
AACTATTACT ATGCGGCACC CCAGCGTCCG GTGGTTCAGA GTTACGGAAC AAGCTACGGC 
GGAGGTGGCT ATGGATCGAA TGCCTATGGT GTTCCTCGTC CAGTCAATTC GTATCAAAGT 
CAAGGCTACA GGGCTGCCGT GGGTAACGAT GAGGTAGATG AGATGTTGCG CCAGCACCAG 
ACAATGGCCA GGACAATAAA CCCGAAGCAA CCAGGCGAAG TCGGTGGATC GGAGAGCCAG 
AAGAGCAACT CTAATCCTCC AACGACGTTG ACACCAGCTC CACAAGAGCA ACCGCAAGAG 
CATCGAGTCC ACAAAAGGTT AGCACATTTC CACAGGTTTG GGCGAGAGGC AGGGTTAAAT 
GCGACCACAT CCAAGGGTTG CGGATGTGGC AGGTTGGATT GTCTGTGCGG CAGGAGTTGT 
CGATGCGGAA GAAGAGGCTT GGAATCTAGA GTGGTTTCTA GTAGAACTTC GGGAACTTGC 
CAGTGCAAAG CCAGTCACCG GAACAAGCGT AGCGTTGAAT ATGGAACCTT GGAGACCATC 
GATGAGGGTT CTCTTAACGA ACTTAGAAGA GAATATAAAC TGGGACTAAA AGAGATCACG 
CTGAGTCCAG ATGAAGATCC CGCCGAGGCC CTGATGCGCT ACAATGCGGC CTCCATTCGC 
GAAGCTTTGG AGCGAGCCAG TATGGAACCG CTGGAGATCG GTGGGGATCA GTATGAAGAG 
GATGCGCAAC AGGAGCCCAT GGAGGAGGAG CAACTACAGC ATGATCCCAA CACAGAGCCC 
CAGTACAATC ATAAGGACTT TGTGCGGCTA ACCACTTCCA CAGCATCTCC GATCACAAGT 
ACCACAGAAG CTGCCACTCC AACAGGCAGT GACTCTACTT CAGAAGCAAC TGTGACACCT 
GAAGTCACCA CCACCACTAG CACATCCACA ACAACAACAA CCGAAAGCAC CAAAGATGAG 
GGACTGGACA TGCAGCAAGA CTCACAGGCT GAGGCTGAGA GTTCTCATGT CACCAAGTCG 
ATATCGAAGC AGGAGGCCGA GATTCATCAG CTGCACAGTA TTGTGGAGGA GCTGAAGAAT 
GAGATCCTTA AACTGAATTT GCGTTGCAGC ACGATAATAT CAAACAATGT GGCCAAAGAA 
CCGGTCACAG AGAAGAACCC ACCCGTGGTG GAGGAACCAT CCAAGCAGGA GGATAAGCCA 
AAGGTGGAGG AGAAAGTGAT TGCCGAAGAG CAAGCCCCAG TGGAGCAGGA GGAAGAGCTG 
GAAGAGGACG AGGATAGTAC CTCTATTAGT ACGACCACAG AGACTCCTTC ACCAAGTGGT 
AGTTACTCAA CAAAACCTGG ACTTTCCCTT GGATCGCCTA GAGTGGATGA ACAAAGTGGC 
TCGTCCAATA AACTGGACTA CGACGACGAT AACAATTGGC AACGCATTCT GGCCAATCGT 
GGCTATGACA CGGACTACCT AACCAAATCC CATGAGCGTC AGTTTGCCCA GGGTCAAAAC 
CTGGAGATGC CCAAGAACTG CAATTACGAT GGAAATGGCA GCCAAGAGTA TGGCCCCTAT 
CCGGAGTTCC AAGCAGATGA ACCCAGCACG GATACGGAGG GCAAGGCTAA AAGGGCTTTA 
AGTGTGAAAC AACAGGCGCA GCTGCTCAAT GCGGCCCTAA ATGATAGTGG AAGCGATTCG 
AGCGATGGCA CCACCACCAC CACCACACCC TCTCCGTACG CGATGAGGGG AAAGTTTGTG 
AGACGGAGGA GCACGGCAAG AAGGGTTCCC ATTCCAAAGA TAGGAAAGGC GAGCGACGAG 
GTATGGGTAC GATCTCCTCG TCAAGCGAAA ATGCCCCAAC GACCCAAGAA GTCCATGTCC 
AAGCCGAAAA AGCAGAGCTC GCAGGTGACT ACGCAGGCCA CCGTGAGCAG CACTAAGCTG 
GACAGTTTGG TGGATGTGCT CAAGGATCTG GTGCGCCTGC AAATTCAAAA GGAGAAAAAA 
TCCAGCCTAT TAAGAACCCA GAGCAATAAC CTTTCGAAAA CCAAACCCAA ATCTATTAAG 
CCCGTCAAAG TCATCAAGCG AAAAAGGTTG CGCAGGAGGC AACACAAGTC TATTGCCACG 
ACCATCAGGA GTCCCATTCA AACGAAAGCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511072.2
CDS	76..4848
Translation	MRLFSLLPLLALLVVQAAGQSEVTSDDPATDAGSTTNSTTDTKPRIPSQDEILGQMPSINPIRTGNPQMDAFYMMFPALGSLLKWGSLFPAYSILGAIPDNLQPTAAASKVVLVLADDATAKTRVARQNPPPNPLGQLMNWPALPQDFQLPSMDLGPQVGSFLAQLPAMPTVPGLLGAAAPVPAPAPAPAAAPPPAPAPAADPPAAPVPDAPQPAILGQAALQNAFTFFNPANFDASSLLGQSVPTFAPPNLDFVAQMQRQFFPGMTPAQPAAAGTDAQASDISEVRVRPEDPYSQEAQMKIKSALEMEQERQQQAQVKDQEQVPLLWFRMPTTQNQDATEEKTLEDLRVEAKLRAFERQVIAELRMLQKIELMAKQMRSSAAAQNGDSPYRISYPLSRTPIHKITRADIEQALRDDYVRRLVNKEAQRRARNSGINTQKANALKRQAKSQDQTLSKEDIVQIMAYAYRMANEQMESEKGKQDKVYAAYRTEQNPMMMQQRQWSEEQAKIQQNQQQIQQNPMMMQQRQWSEEQAKIQQNQQQIQQNPMMMQQRQWSEEQAKIQQNQQQIQQNPMMMQQRQWSEEQAKIQQNQQQIQQNPMMVQQRQWSEEQAKIQQNQQQIQQNPMMMQQRQWSEEQAKIQHDQQMAQQMAQQGLMMTEQRQRQWSEDQAKIQQAQQMAQQTPMMMPQMQQRQWTEDPQMVQQMQQRQWAEDQTRMQMAQQNPMMQQQRQMAENPQMMQQRQWSEEQTKIEQAQQMAQQNQMMMQQMQQRQWSEDQAQIQQQQRQMMQQTPMMMKERQWAEENPQSVQQQGPMMMQQQMPSMMQREVEDEDNKAEDDLVGEAGPQMPENEGTARHKVDALGVGGNKRKKSKSKSAPPTVINYYYAAPQRPVVQSYGTSYGGGGYGSNAYGVPRPVNSYQSQGYRAAVGNDEVDEMLRQHQTMARTINPKQPGEVGGSESQKSNSNPPTTLTPAPQEQPQEHRVHKRLAHFHRFGREAGLNATTSKGCGCGRLDCLCGRSCRCGRRGLESRVVSSRTSGTCQCKASHRNKRSVEYGTLETIDEGSLNELRREYKLGLKEITLSPDEDPAEALMRYNAASIREALERASMEPLEIGGDQYEEDAQQEPMEEEQLQHDPNTEPQYNHKDFVRLTTSTASPITSTTEAATPTGSDSTSEATVTPEVTTTTSTSTTTTTESTKDEGLDMQQDSQAEAESSHVTKSISKQEAEIHQLHSIVEELKNEILKLNLRCSTIISNNVAKEPVTEKNPPVVEEPSKQEDKPKVEEKVIAEEQAPVEQEEELEEDEDSTSISTTTETPSPSGSYSTKPGLSLGSPRVDEQSGSSNKLDYDDDNNWQRILANRGYDTDYLTKSHERQFAQGQNLEMPKNCNYDGNGSQEYGPYPEFQADEPSTDTEGKAKRALSVKQQAQLLNAALNDSGSDSSDGTTTTTTPSPYAMRGKFVRRRSTARRVPIPKIGKASDEVWVRSPRQAKMPQRPKKSMSKPKKQSSQVTTQATVSSTKLDSLVDVLKDLVRLQIQKEKKSSLLRTQSNNLSKTKPKSIKPVKVIKRKRLRRRQHKSIATTIRSPIQTKA

Target ORF information:

RefSeq Version	NM_078517.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster defective chorion 1 (dec-1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078517.3

ORF Insert Sequence:

ATGAGATTGT TTAGCCTTCT GCCGCTCCTG GCGCTTCTTG TCGTCCAGGC TGCCGGACAA 
AGTGAAGTAA CCTCTGATGA TCCTGCAACG GATGCTGGAT CAACGACCAA TTCCACCACG 
GACACCAAAC CAAGGATTCC CAGTCAGGAT GAGATCCTTG GCCAGATGCC GTCCATTAAT 
CCCATCCGCA CTGGCAATCC CCAGATGGAC GCATTTTACA TGATGTTCCC GGCGTTGGGC 
AGCCTGCTCA AGTGGGGCAG CCTTTTCCCA GCCTACTCGA TTCTGGGCGC CATTCCCGAC 
AATCTACAGC CTACAGCGGC AGCCTCCAAG GTGGTCCTTG TCCTGGCCGA TGATGCGACG 
GCCAAGACTC GAGTTGCCCG TCAAAATCCG CCACCAAATC CACTTGGTCA GCTAATGAAT 
TGGCCCGCTC TGCCGCAGGA CTTCCAACTG CCTTCCATGG ATCTGGGACC GCAAGTGGGC 
TCGTTTTTGG CCCAACTGCC TGCTATGCCT ACCGTGCCTG GTCTTCTGGG TGCCGCTGCT 
CCAGTTCCTG CTCCAGCTCC TGCTCCCGCT GCTGCTCCTC CTCCGGCGCC AGCTCCAGCT 
GCAGATCCTC CGGCAGCACC TGTTCCAGAT GCACCCCAAC CAGCCATACT GGGACAAGCC 
GCTCTGCAGA ACGCTTTCAC CTTCTTTAAC CCGGCTAACT TTGATGCCTC CAGTCTTCTG 
GGCCAGAGTG TACCCACATT TGCTCCTCCA AATCTTGATT TCGTCGCTCA AATGCAAAGG 
CAATTCTTCC CAGGAATGAC ACCGGCACAA CCTGCTGCCG CTGGCACGGA TGCCCAGGCC 
TCCGACATTT CCGAGGTAAG GGTACGTCCT GAGGATCCGT ATTCGCAAGA GGCACAGATG 
AAGATCAAAT CGGCACTTGA AATGGAGCAG GAGAGGCAAC AACAGGCTCA GGTCAAGGAT 
CAGGAGCAAG TGCCTCTCCT CTGGTTCCGA ATGCCCACAA CTCAGAATCA GGATGCGACT 
GAAGAAAAGA CTCTGGAGGA TCTGCGGGTC GAGGCCAAAT TGAGGGCATT CGAGCGCCAG 
GTGATAGCCG AATTGAGAAT GCTGCAGAAG ATCGAACTTA TGGCTAAGCA GATGAGGTCC 
AGCGCCGCAG CTCAAAACGG AGATTCACCC TACAGAATCA GTTATCCACT GAGTCGGACA 
CCCATTCACA AGATCACCCG TGCTGATATT GAGCAAGCCC TTCGGGATGA TTATGTCCGC 
CGATTGGTCA ACAAGGAAGC GCAACGAAGG GCTAGAAATT CCGGCATAAA CACCCAGAAG 
GCAAATGCCT TGAAGCGACA GGCCAAATCC CAGGATCAGA CTCTGTCCAA GGAGGATATC 
GTCCAGATTA TGGCGTATGC CTATCGCATG GCCAACGAAC AGATGGAGAG CGAGAAGGGC 
AAGCAGGACA AGGTTTACGC GGCCTACAGG ACGGAACAGA ATCCAATGAT GATGCAGCAA 
AGACAATGGT CGGAGGAGCA GGCCAAGATC CAACAGAATC AACAGCAGAT CCAGCAAAAT 
CCAATGATGA TGCAGCAACG ACAATGGTCG GAGGAGCAGG CCAAGATCCA ACAGAATCAA 
CAGCAGATCC AGCAAAATCC AATGATGATG CAGCAACGAC AATGGTCGGA GGAGCAGGCC 
AAGATCCAAC AGAATCAACA GCAGATCCAG CAAAATCCAA TGATGATGCA GCAAAGACAA 
TGGTCGGAGG AGCAGGCTAA GATCCAACAG AATCAACAGC AGATCCAGCA AAATCCAATG 
ATGGTGCAGC AAAGACAATG GTCGGAGGAG CAGGCCAAGA TCCAACAGAA TCAACAGCAG 
ATCCAGCAAA ATCCAATGAT GATGCAGCAA AGACAATGGT CGGAGGAGCA GGCCAAGATT 
CAGCATGATC AACAGATGGC ACAACAGATG GCACAGCAAG GTCTCATGAT GACGGAGCAG 
AGGCAAAGGC AGTGGTCAGA AGATCAGGCC AAAATTCAGC AGGCTCAACA GATGGCCCAA 
CAGACACCCA TGATGATGCC ACAGATGCAA CAAAGGCAGT GGACAGAGGA TCCCCAAATG 
GTTCAGCAGA TGCAACAGAG GCAGTGGGCA GAAGATCAGA CCAGGATGCA AATGGCCCAA 
CAGAATCCAA TGATGCAACA GCAGCGACAG ATGGCAGAGA ATCCGCAAAT GATGCAGCAA 
AGGCAATGGT CCGAGGAGCA GACCAAAATC GAACAGGCTC AGCAGATGGC ACAACAGAAT 
CAAATGATGA TGCAGCAGAT GCAGCAAAGA CAATGGTCGG AGGATCAGGC CCAGATTCAA 
CAGCAGCAGA GACAGATGAT GCAGCAGACC CCCATGATGA TGAAGGAGCG CCAGTGGGCG 
GAGGAGAACC CCCAGTCTGT CCAGCAGCAA GGGCCCATGA TGATGCAGCA GCAGATGCCA 
TCGATGATGC AGCGTGAAGT AGAGGACGAA GATAATAAGG CAGAGGATGA CTTAGTTGGC 
GAGGCGGGAC CCCAAATGCC GGAGAACGAA GGCACCGCCA GGCACAAAGT CGATGCCCTG 
GGAGTTGGCG GCAACAAGCG CAAGAAGTCC AAGTCCAAGT CGGCGCCGCC AACGGTAATT 
AACTATTACT ATGCGGCACC CCAGCGTCCG GTGGTTCAGA GTTACGGAAC AAGCTACGGC 
GGAGGTGGCT ATGGATCGAA TGCCTATGGT GTTCCTCGTC CAGTCAATTC GTATCAAAGT 
CAAGGCTACA GGGCTGCCGT GGGTAACGAT GAGGTAGATG AGATGTTGCG CCAGCACCAG 
ACAATGGCCA GGACAATAAA CCCGAAGCAA CCAGGCGAAG TCGGTGGATC GGAGAGCCAG 
AAGAGCAACT CTAATCCTCC AACGACGTTG ACACCAGCTC CACAAGAGCA ACCGCAAGAG 
CATCGAGTCC ACAAAAGGTT AGCACATTTC CACAGGTTTG GGCGAGAGGC AGGGTTAAAT 
GCGACCACAT CCAAGGGTTG CGGATGTGGC AGGTTGGATT GTCTGTGCGG CAGGAGTTGT 
CGATGCGGAA GAAGAGGCTT GGAATCTAGA GTGGTTTCTA GTAGAACTTC GGGAACTTGC 
CAGTGCAAAG CCAGTCACCG GAACAAGCGT AGCGTTGAAT ATGGAACCTT GGAGACCATC 
GATGAGGGTT CTCTTAACGA ACTTAGAAGA GAATATAAAC TGGGACTAAA AGAGATCACG 
CTGAGTCCAG ATGAAGATCC CGCCGAGGCC CTGATGCGCT ACAATGCGGC CTCCATTCGC 
GAAGCTTTGG AGCGAGCCAG TATGGAACCG CTGGAGATCG GTGGGGATCA GTATGAAGAG 
GATGCGCAAC AGGAGCCCAT GGAGGAGGAG CAACTACAGC ATGATCCCAA CACAGAGCCC 
CAGTACAATC ATAAGGACTT TGTGCGGCTA ACCACTTCCA CAGCATCTCC GATCACAAGT 
ACCACAGAAG CTGCCACTCC AACAGGCAGT GACTCTACTT CAGAAGCAAC TGTGACACCT 
GAAGTCACCA CCACCACTAG CACATCCACA ACAACAACAA CCGAAAGCAC CAAAGATGAG 
GGACTGGACA TGCAGCAAGA CTCACAGGCT GAGGCTGAGA GTTCTCATGT CACCAAGTCG 
ATATCGAAGC AGGAGGCCGA GATTCATCAG CTGCACAGTA TTGTGGAGGA GCTGAAGAAT 
GAGATCCTTA AACTGAATTT GCGTTGCAGC ACGATAATAT CAAACAATGT GGCCAAAGAA 
CCGGTCACAG AGAAGAACCC ACCCGTGGTG GAGGAACCAT CCAAGCAGGA GGATAAGCCA 
AAGGTGGAGG AGAAAGTGAT TGCCGAAGAG CAAGCCCCAG TGGAGCAGGA GGAAGAGCTG 
GAAGAGGACG AGGATAGTAC CTCTATTAGT ACGACCACAG AGACTCCTTC ACCAAGTGGT 
AGTTACTCAA CAAAACCTGG ACTTTCCCTT GGATCGCCTA GAGTGGATGA ACAAAGTGGC 
TCGTCCAATA AACTGGACTA CGACGACGAT AACAATTGGC AACGCATTCT GGCCAATCGT 
GGCTATGACA CGGACTACCT AACCAAATCC CATGAGCGTC AGTTTGCCCA GGGTCAAAAC 
CTGGAGATGC CCAAGAACTG CAATTACGAT GGAAATGGCA GCCAAGAGTA TGGCCCCTAT 
CCGGAGTTCC AAGCAGATGA ACCCAGCACG GATACGGAGG GCAAGGCTAA AAGGGCTTTA 
AGTGTGAAAC AACAGGCGCA GCTGCTCAAT GCGGCCCTAA ATGATAGTGG AAGCGATTCG 
AGCGATGGCA CCACCACCAC CACCACACCC TCTCCGTACG CGATGAGGGG AAAGTTTGTG 
AGACGGAGGA GCACGGCAAG AAGGGTTCCC ATTCCAAAGA TAGGAAAGGC GAGCGACGAG 
GTATGGGTAC GATCTCCTCG TCAAGCGAAA ATGCCCCAAC GACCCAAGAA GTCCATGTCC 
AAGCCGAAAA AGCAGAGCTC GCAGGTGACT ACGCAGGCCA CCGTGAGCAG CACTAAGCTG 
GACAGTTTGG TGGATGTGCT CAAGGATCTG GTGCGCCTGC AAATTCAAAA GGAGAAAAAA 
TCCAGCCTAT TAAGAACCCA GAGCAATAAC CTTTCGAAAA CCAAACCCAA ATCTATTAAG 
CCCGTCAAAG TCATCAAGCG AAAAAGGTTG CGCAGGAGGC AACACAAGTC TATTGCCACG 
ACCATCAGGA GTCCCATTCA AACGAAAGCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dec-1 ( NM_167133.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727203.1 Drosophila melanogaster defective chorion 1 (dec-1), transcript variant C, mRNA.

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CloneID	OFa01039
Clone ID Related Accession (Same CDS sequence)	NM_167133.2
Accession Version	NM_167133.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	defective chorion 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On May 8, 2012 this sequence version replaced NM_167133.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGATTGT TTAGCCTTCT GCCGCTCCTG GCGCTTCTTG TCGTCCAGGC TGCCGGACAA 
AGTGAAGTAA CCTCTGATGA TCCTGCAACG GATGCTGGAT CAACGACCAA TTCCACCACG 
GACACCAAAC CAAGGATTCC CAGTCAGGAT GAGATCCTTG GCCAGATGCC GTCCATTAAT 
CCCATCCGCA CTGGCAATCC CCAGATGGAC GCATTTTACA TGATGTTCCC GGCGTTGGGC 
AGCCTGCTCA AGTGGGGCAG CCTTTTCCCA GCCTACTCGA TTCTGGGCGC CATTCCCGAC 
AATCTACAGC CTACAGCGGC AGCCTCCAAG GTGGTCCTTG TCCTGGCCGA TGATGCGACG 
GCCAAGACTC GAGTTGCCCG TCAAAATCCG CCACCAAATC CACTTGGTCA GCTAATGAAT 
TGGCCCGCTC TGCCGCAGGA CTTCCAACTG CCTTCCATGG ATCTGGGACC GCAAGTGGGC 
TCGTTTTTGG CCCAACTGCC TGCTATGCCT ACCGTGCCTG GTCTTCTGGG TGCCGCTGCT 
CCAGTTCCTG CTCCAGCTCC TGCTCCCGCT GCTGCTCCTC CTCCGGCGCC AGCTCCAGCT 
GCAGATCCTC CGGCAGCACC TGTTCCAGAT GCACCCCAAC CAGCCATACT GGGACAAGCC 
GCTCTGCAGA ACGCTTTCAC CTTCTTTAAC CCGGCTAACT TTGATGCCTC CAGTCTTCTG 
GGCCAGAGTG TACCCACATT TGCTCCTCCA AATCTTGATT TCGTCGCTCA AATGCAAAGG 
CAATTCTTCC CAGGAATGAC ACCGGCACAA CCTGCTGCCG CTGGCACGGA TGCCCAGGCC 
TCCGACATTT CCGAGGTAAG GGTACGTCCT GAGGATCCGT ATTCGCAAGA GGCACAGATG 
AAGATCAAAT CGGCACTTGA AATGGAGCAG GAGAGGCAAC AACAGGCTCA GGTCAAGGAT 
CAGGAGCAAG TGCCTCTCCT CTGGTTCCGA ATGCCCACAA CTCAGAATCA GGATGCGACT 
GAAGAAAAGA CTCTGGAGGA TCTGCGGGTC GAGGCCAAAT TGAGGGCATT CGAGCGCCAG 
GTGATAGCCG AATTGAGAAT GCTGCAGAAG ATCGAACTTA TGGCTAAGCA GATGAGGTCC 
AGCGCCGCAG CTCAAAACGG AGATTCACCC TACAGAATCA GTTATCCACT GAGTCGGACA 
CCCATTCACA AGATCACCCG TGCTGATATT GAGCAAGCCC TTCGGGATGA TTATGTCCGC 
CGATTGGTCA ACAAGGAAGC GCAACGAAGG GCTAGAAATT CCGGCATAAA CACCCAGAAG 
GCAAATGCCT TGAAGCGACA GGCCAAATCC CAGGATCAGA CTCTGTCCAA GGAGGATATC 
GTCCAGATTA TGGCGTATGC CTATCGCATG GCCAACGAAC AGATGGAGAG CGAGAAGGGC 
AAGCAGGACA AGGTTTACGC GGCCTACAGG ACGGAACAGA ATCCAATGAT GATGCAGCAA 
AGACAATGGT CGGAGGAGCA GGCCAAGATC CAACAGAATC AACAGCAGAT CCAGCAAAAT 
CCAATGATGA TGCAGCAACG ACAATGGTCG GAGGAGCAGG CCAAGATCCA ACAGAATCAA 
CAGCAGATCC AGCAAAATCC AATGATGATG CAGCAACGAC AATGGTCGGA GGAGCAGGCC 
AAGATCCAAC AGAATCAACA GCAGATCCAG CAAAATCCAA TGATGATGCA GCAAAGACAA 
TGGTCGGAGG AGCAGGCTAA GATCCAACAG AATCAACAGC AGATCCAGCA AAATCCAATG 
ATGGTGCAGC AAAGACAATG GTCGGAGGAG CAGGCCAAGA TCCAACAGAA TCAACAGCAG 
ATCCAGCAAA ATCCAATGAT GATGCAGCAA AGACAATGGT CGGAGGAGCA GGCCAAGATT 
CAGCATGATC AACAGATGGC ACAACAGATG GCACAGCAAG GTCTCATGAT GACGGAGCAG 
AGGCAAAGGC AGTGGTCAGA AGATCAGGCC AAAATTCAGC AGGCTCAACA GATGGCCCAA 
CAGACACCCA TGATGATGCC ACAGATGCAA CAAAGGCAGT GGACAGAGGA TCCCCAAATG 
GTTCAGCAGA TGCAACAGAG GCAGTGGGCA GAAGATCAGA CCAGGATGCA AATGGCCCAA 
CAGAATCCAA TGATGCAACA GCAGCGACAG ATGGCAGAGA ATCCGCAAAT GATGCAGCAA 
AGGCAATGGT CCGAGGAGCA GACCAAAATC GAACAGGCTC AGCAGATGGC ACAACAGAAT 
CAAATGATGA TGCAGCAGAT GCAGCAAAGA CAATGGTCGG AGGATCAGGC CCAGATTCAA 
CAGCAGCAGA GACAGATGAT GCAGCAGACC CCCATGATGA TGAAGGAGCG CCAGTGGGCG 
GAGGAGAACC CCCAGTCTGT CCAGCAGCAA GGGCCCATGA TGATGCAGCA GCAGATGCCA 
TCGATGATGC AGCGTGAAGT AGAGGACGAA GATAATAAGG CAGAGGATGA CTTAGTTGGC 
GAGGCGGGAC CCCAAATGCC GGAGAACGAA GGCACCGCCA GGCACAAAGT CGATGCCCTG 
GGAGTTGGCG GCAACAAGCG CAAGAAGTCC AAGTCCAAGT CGGCGCCGCC AACGGTAATT 
AACTATTACT ATGCGGCACC CCAGCGTCCG GTGGTTCAGA GTTACGGAAC AAGCTACGGC 
GGAGGTGGCT ATGGATCGAA TGCCTATGGT GTTCCTCGTC CAGTCAATTC GTATCAAAGT 
CAAGGCTACA GGGCTGCCGT GGGTAACGAT GAGGTAGATG AGATGTTGCG CCAGCACCAG 
ACAATGGCCA GGGCGACGCA TTTCAGACAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727203.1
CDS	76..2928
Translation	MRLFSLLPLLALLVVQAAGQSEVTSDDPATDAGSTTNSTTDTKPRIPSQDEILGQMPSINPIRTGNPQMDAFYMMFPALGSLLKWGSLFPAYSILGAIPDNLQPTAAASKVVLVLADDATAKTRVARQNPPPNPLGQLMNWPALPQDFQLPSMDLGPQVGSFLAQLPAMPTVPGLLGAAAPVPAPAPAPAAAPPPAPAPAADPPAAPVPDAPQPAILGQAALQNAFTFFNPANFDASSLLGQSVPTFAPPNLDFVAQMQRQFFPGMTPAQPAAAGTDAQASDISEVRVRPEDPYSQEAQMKIKSALEMEQERQQQAQVKDQEQVPLLWFRMPTTQNQDATEEKTLEDLRVEAKLRAFERQVIAELRMLQKIELMAKQMRSSAAAQNGDSPYRISYPLSRTPIHKITRADIEQALRDDYVRRLVNKEAQRRARNSGINTQKANALKRQAKSQDQTLSKEDIVQIMAYAYRMANEQMESEKGKQDKVYAAYRTEQNPMMMQQRQWSEEQAKIQQNQQQIQQNPMMMQQRQWSEEQAKIQQNQQQIQQNPMMMQQRQWSEEQAKIQQNQQQIQQNPMMMQQRQWSEEQAKIQQNQQQIQQNPMMVQQRQWSEEQAKIQQNQQQIQQNPMMMQQRQWSEEQAKIQHDQQMAQQMAQQGLMMTEQRQRQWSEDQAKIQQAQQMAQQTPMMMPQMQQRQWTEDPQMVQQMQQRQWAEDQTRMQMAQQNPMMQQQRQMAENPQMMQQRQWSEEQTKIEQAQQMAQQNQMMMQQMQQRQWSEDQAQIQQQQRQMMQQTPMMMKERQWAEENPQSVQQQGPMMMQQQMPSMMQREVEDEDNKAEDDLVGEAGPQMPENEGTARHKVDALGVGGNKRKKSKSKSAPPTVINYYYAAPQRPVVQSYGTSYGGGGYGSNAYGVPRPVNSYQSQGYRAAVGNDEVDEMLRQHQTMARATHFRQ

Target ORF information:

RefSeq Version	NM_167133.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster defective chorion 1 (dec-1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167133.2

ORF Insert Sequence:

ATGAGATTGT TTAGCCTTCT GCCGCTCCTG GCGCTTCTTG TCGTCCAGGC TGCCGGACAA 
AGTGAAGTAA CCTCTGATGA TCCTGCAACG GATGCTGGAT CAACGACCAA TTCCACCACG 
GACACCAAAC CAAGGATTCC CAGTCAGGAT GAGATCCTTG GCCAGATGCC GTCCATTAAT 
CCCATCCGCA CTGGCAATCC CCAGATGGAC GCATTTTACA TGATGTTCCC GGCGTTGGGC 
AGCCTGCTCA AGTGGGGCAG CCTTTTCCCA GCCTACTCGA TTCTGGGCGC CATTCCCGAC 
AATCTACAGC CTACAGCGGC AGCCTCCAAG GTGGTCCTTG TCCTGGCCGA TGATGCGACG 
GCCAAGACTC GAGTTGCCCG TCAAAATCCG CCACCAAATC CACTTGGTCA GCTAATGAAT 
TGGCCCGCTC TGCCGCAGGA CTTCCAACTG CCTTCCATGG ATCTGGGACC GCAAGTGGGC 
TCGTTTTTGG CCCAACTGCC TGCTATGCCT ACCGTGCCTG GTCTTCTGGG TGCCGCTGCT 
CCAGTTCCTG CTCCAGCTCC TGCTCCCGCT GCTGCTCCTC CTCCGGCGCC AGCTCCAGCT 
GCAGATCCTC CGGCAGCACC TGTTCCAGAT GCACCCCAAC CAGCCATACT GGGACAAGCC 
GCTCTGCAGA ACGCTTTCAC CTTCTTTAAC CCGGCTAACT TTGATGCCTC CAGTCTTCTG 
GGCCAGAGTG TACCCACATT TGCTCCTCCA AATCTTGATT TCGTCGCTCA AATGCAAAGG 
CAATTCTTCC CAGGAATGAC ACCGGCACAA CCTGCTGCCG CTGGCACGGA TGCCCAGGCC 
TCCGACATTT CCGAGGTAAG GGTACGTCCT GAGGATCCGT ATTCGCAAGA GGCACAGATG 
AAGATCAAAT CGGCACTTGA AATGGAGCAG GAGAGGCAAC AACAGGCTCA GGTCAAGGAT 
CAGGAGCAAG TGCCTCTCCT CTGGTTCCGA ATGCCCACAA CTCAGAATCA GGATGCGACT 
GAAGAAAAGA CTCTGGAGGA TCTGCGGGTC GAGGCCAAAT TGAGGGCATT CGAGCGCCAG 
GTGATAGCCG AATTGAGAAT GCTGCAGAAG ATCGAACTTA TGGCTAAGCA GATGAGGTCC 
AGCGCCGCAG CTCAAAACGG AGATTCACCC TACAGAATCA GTTATCCACT GAGTCGGACA 
CCCATTCACA AGATCACCCG TGCTGATATT GAGCAAGCCC TTCGGGATGA TTATGTCCGC 
CGATTGGTCA ACAAGGAAGC GCAACGAAGG GCTAGAAATT CCGGCATAAA CACCCAGAAG 
GCAAATGCCT TGAAGCGACA GGCCAAATCC CAGGATCAGA CTCTGTCCAA GGAGGATATC 
GTCCAGATTA TGGCGTATGC CTATCGCATG GCCAACGAAC AGATGGAGAG CGAGAAGGGC 
AAGCAGGACA AGGTTTACGC GGCCTACAGG ACGGAACAGA ATCCAATGAT GATGCAGCAA 
AGACAATGGT CGGAGGAGCA GGCCAAGATC CAACAGAATC AACAGCAGAT CCAGCAAAAT 
CCAATGATGA TGCAGCAACG ACAATGGTCG GAGGAGCAGG CCAAGATCCA ACAGAATCAA 
CAGCAGATCC AGCAAAATCC AATGATGATG CAGCAACGAC AATGGTCGGA GGAGCAGGCC 
AAGATCCAAC AGAATCAACA GCAGATCCAG CAAAATCCAA TGATGATGCA GCAAAGACAA 
TGGTCGGAGG AGCAGGCTAA GATCCAACAG AATCAACAGC AGATCCAGCA AAATCCAATG 
ATGGTGCAGC AAAGACAATG GTCGGAGGAG CAGGCCAAGA TCCAACAGAA TCAACAGCAG 
ATCCAGCAAA ATCCAATGAT GATGCAGCAA AGACAATGGT CGGAGGAGCA GGCCAAGATT 
CAGCATGATC AACAGATGGC ACAACAGATG GCACAGCAAG GTCTCATGAT GACGGAGCAG 
AGGCAAAGGC AGTGGTCAGA AGATCAGGCC AAAATTCAGC AGGCTCAACA GATGGCCCAA 
CAGACACCCA TGATGATGCC ACAGATGCAA CAAAGGCAGT GGACAGAGGA TCCCCAAATG 
GTTCAGCAGA TGCAACAGAG GCAGTGGGCA GAAGATCAGA CCAGGATGCA AATGGCCCAA 
CAGAATCCAA TGATGCAACA GCAGCGACAG ATGGCAGAGA ATCCGCAAAT GATGCAGCAA 
AGGCAATGGT CCGAGGAGCA GACCAAAATC GAACAGGCTC AGCAGATGGC ACAACAGAAT 
CAAATGATGA TGCAGCAGAT GCAGCAAAGA CAATGGTCGG AGGATCAGGC CCAGATTCAA 
CAGCAGCAGA GACAGATGAT GCAGCAGACC CCCATGATGA TGAAGGAGCG CCAGTGGGCG 
GAGGAGAACC CCCAGTCTGT CCAGCAGCAA GGGCCCATGA TGATGCAGCA GCAGATGCCA 
TCGATGATGC AGCGTGAAGT AGAGGACGAA GATAATAAGG CAGAGGATGA CTTAGTTGGC 
GAGGCGGGAC CCCAAATGCC GGAGAACGAA GGCACCGCCA GGCACAAAGT CGATGCCCTG 
GGAGTTGGCG GCAACAAGCG CAAGAAGTCC AAGTCCAAGT CGGCGCCGCC AACGGTAATT 
AACTATTACT ATGCGGCACC CCAGCGTCCG GTGGTTCAGA GTTACGGAAC AAGCTACGGC 
GGAGGTGGCT ATGGATCGAA TGCCTATGGT GTTCCTCGTC CAGTCAATTC GTATCAAAGT 
CAAGGCTACA GGGCTGCCGT GGGTAACGAT GAGGTAGATG AGATGTTGCG CCAGCACCAG 
ACAATGGCCA GGGCGACGCA TTTCAGACAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dec-1 ( NM_001258641.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245570.1 Drosophila melanogaster defective chorion 1 (dec-1), transcript variant E, mRNA.

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CloneID	OFa03016
Clone ID Related Accession (Same CDS sequence)	NM_001258641.1
Accession Version	NM_001258641.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	defective chorion 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGATTGT TTAGCCTTCT GCCGCTCCTG GCGCTTCTTG TCGTCCAGGC TGCCGGACAA 
AGTGAAGTAA CCTCTGATGA TCCTGCAACG GATGCTGGAT CAACGACCAA TTCCACCACG 
GACACCAAAC CAAGGATTCC CAGTCAGGAT GAGATGGACG CATTTTACAT GATGTTCCCG 
GCGTTGGGCA GCCTGCTCAA GTGGGGCAGC CTTTTCCCAG CCTACTCGAT TCTGGGCGCC 
ATTCCCGACA ATCTACAGCC TACAGCGGCA GCCTCCAAGG TGGTCCTTGT CCTGGCCGAT 
GATGCGACGG CCAAGACTCG AGTTGCCCGT CAAAATCCGC CACCAAATCC ACTTGGTCAG 
CTAATGAATT GGCCCGCTCT GCCGCAGGAC TTCCAACTGC CTTCCATGGA TCTGGGACCG 
CAAGTGGGCT CGTTTTTGGC CCAACTGCCT GCTATGCCTA CCGTGCCTGG TCTTCTGGGT 
GCCGCTGCTC CAGTTCCTGC TCCAGCTCCT GCTCCCGCTG CTGCTCCTCC TCCGGCGCCA 
GCTCCAGCTG CAGATCCTCC GGCAGCACCT GTTCCAGATG CACCCCAACC AGCCATACTG 
GGACAAGCCG CTCTGCAGAA CGCTTTCACC TTCTTTAACC CGGCTAACTT TGATGCCTCC 
AGTCTTCTGG GCCAGAGTGT ACCCACATTT GCTCCTCCAA ATCTTGATTT CGTCGCTCAA 
ATGCAAAGGC AATTCTTCCC AGGAATGACA CCGGCACAAC CTGCTGCCGC TGGCACGGAT 
GCCCAGGCCT CCGACATTTC CGAGGTAAGG GTACGTCCTG AGGATCCGTA TTCGCAAGAG 
GCACAGATGA AGATCAAATC GGCACTTGAA ATGGAGCAGG AGAGGCAACA ACAGGCTCAG 
GTCAAGGATC AGGAGCAAGT GCCTCTCCTC TGGTTCCGAA TGCCCACAAC TCAGAATCAG 
GATGCGACTG AAGAAAAGAC TCTGGAGGAT CTGCGGGTCG AGGCCAAATT GAGGGCATTC 
GAGCGCCAGG TGATAGCCGA ATTGAGAATG CTGCAGAAGA TCGAACTTAT GGCTAAGCAG 
ATGAGGTCCA GCGCCGCAGC TCAAAACGGA GATTCACCCT ACAGAATCAG TTATCCACTG 
AGTCGGACAC CCATTCACAA GATCACCCGT GCTGATATTG AGCAAGCCCT TCGGGATGAT 
TATGTCCGCC GATTGGTCAA CAAGGAAGCG CAACGAAGGG CTAGAAATTC CGGCATAAAC 
ACCCAGAAGG CAAATGCCTT GAAGCGACAG GCCAAATCCC AGGATCAGAC TCTGTCCAAG 
GAGGATATCG TCCAGATTAT GGCGTATGCC TATCGCATGG CCAACGAACA GATGGAGAGC 
GAGAAGGGCA AGCAGGACAA GGTTTACGCG GCCTACAGGA CGGAACAGAA TCCAATGATG 
ATGCAGCAAA GACAATGGTC GGAGGAGCAG GCCAAGATCC AACAGAATCA ACAGCAGATC 
CAGCAAAATC CAATGATGAT GCAGCAACGA CAATGGTCGG AGGAGCAGGC CAAGATCCAA 
CAGAATCAAC AGCAGATCCA GCAAAATCCA ATGATGATGC AGCAACGACA ATGGTCGGAG 
GAGCAGGCCA AGATCCAACA GAATCAACAG CAGATCCAGC AAAATCCAAT GATGATGCAG 
CAAAGACAAT GGTCGGAGGA GCAGGCTAAG ATCCAACAGA ATCAACAGCA GATCCAGCAA 
AATCCAATGA TGGTGCAGCA AAGACAATGG TCGGAGGAGC AGGCCAAGAT CCAACAGAAT 
CAACAGCAGA TCCAGCAAAA TCCAATGATG ATGCAGCAAA GACAATGGTC GGAGGAGCAG 
GCCAAGATTC AGCATGATCA ACAGATGGCA CAACAGATGG CACAGCAAGG TCTCATGATG 
ACGGAGCAGA GGCAAAGGCA GTGGTCAGAA GATCAGGCCA AAATTCAGCA GGCTCAACAG 
ATGGCCCAAC AGACACCCAT GATGATGCCA CAGATGCAAC AAAGGCAGTG GACAGAGGAT 
CCCCAAATGG TTCAGCAGAT GCAACAGAGG CAGTGGGCAG AAGATCAGAC CAGGATGCAA 
ATGGCCCAAC AGAATCCAAT GATGCAACAG CAGCGACAGA TGGCAGAGAA TCCGCAAATG 
ATGCAGCAAA GGCAATGGTC CGAGGAGCAG ACCAAAATCG AACAGGCTCA GCAGATGGCA 
CAACAGAATC AAATGATGAT GCAGCAGATG CAGCAAAGAC AATGGTCGGA GGATCAGGCC 
CAGATTCAAC AGCAGCAGAG ACAGATGATG CAGCAGACCC CCATGATGAT GAAGGAGCGC 
CAGTGGGCGG AGGAGAACCC CCAGTCTGTC CAGCAGCAAG GGCCCATGAT GATGCAGCAG 
CAGATGCCAT CGATGATGCA GCGTGAAGTA GAGGACGAAG ATAATAAGGC AGAGGATGAC 
TTAGTTGGCG AGGCGGGACC CCAAATGCCG GAGAACGAAG GCACCGCCAG GCACAAAGTC 
GATGCCCTGG GAGTTGGCGG CAACAAGCGC AAGAAGTCCA AGTCCAAGTC GGCGCCGCCA 
ACGGTAATTA ACTATTACTA TGCGGCACCC CAGCGTCCGG TGGTTCAGAG TTACGGAACA 
AGCTACGGCG GAGGTGGCTA TGGATCGAAT GCCTATGGTG TTCCTCGTCC AGTCAATTCG 
TATCAAAGTC AAGGCTACAG GGCTGCCGTG GGTAACGATG AGGTAGATGA GATGTTGCGC 
CAGCACCAGA CAATGGCCAG GACAATAAAC CCGAAGCAAC CAGGCGAAGT CGGTGGATCG 
GAGAGCCAGA AGAGCAACTC TAATCCTCCA ACGACGTTGA CACCAGCTCC ACAAGAGCAA 
CCGCAAGAGC ATCGAGTCCA CAAAAGTCCA TCATCAGCAC CATCCGAAAC CGAAATCGAA 
AACGCACCAT CATCTGACCC CCAAGTGGGT TCCATTTTCA CCTACGGCGA GGGATTGCTG 
CATCCGTTTA TGGGTCTGCT GCCGGTGGAG AGGCCCGATG ATCCGTGGAA CCAGAAGCCA 
TACGATCCTC ACCATCCTCT CTACACGGGC GGTGGGAGCT ACGATGCCTA TCTGAGGGAC 
GGTCGCCATC GAAGGGACAC GCACATCATG GGTCAAGGTA CGCAGCATGG CATCCTGACA 
CCCGGCATGT TGGAGAGACT TCTTCGGATC AAGATGGATT TCCAGCGTAG ATTTCCCCAT 
TTGTACAAGG GTATGCTGAA TCACCATACG AATCTTACCC GTGTAGAGGT ACAGCCTCCG 
GTTCTGGGCA AGATTTCTAA GCCGAAAACT AAGACCAAGC CCAAGAATGA GGACGAACCC 
GTCTTCGAAT TGGGAGCCGC TGAGCGTAGC CTGTTCGAGG ACGAGACCAA CGATTCTTTA 
GAGAAAGATC CCGAACCAGA ACCGGATGAG GAGGATGACC GGGATGTGGA AGAGCCCAGC 
GAGAGCAGTG AACCTAGGGG ATTCAGCAGC AAAAAAAGCC GGGATGAGAA CGACATCGAT 
TACTTCAATT TCGATGACGA TGATGTTGAT GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245570.1
CDS	76..3651
Translation	MRLFSLLPLLALLVVQAAGQSEVTSDDPATDAGSTTNSTTDTKPRIPSQDEMDAFYMMFPALGSLLKWGSLFPAYSILGAIPDNLQPTAAASKVVLVLADDATAKTRVARQNPPPNPLGQLMNWPALPQDFQLPSMDLGPQVGSFLAQLPAMPTVPGLLGAAAPVPAPAPAPAAAPPPAPAPAADPPAAPVPDAPQPAILGQAALQNAFTFFNPANFDASSLLGQSVPTFAPPNLDFVAQMQRQFFPGMTPAQPAAAGTDAQASDISEVRVRPEDPYSQEAQMKIKSALEMEQERQQQAQVKDQEQVPLLWFRMPTTQNQDATEEKTLEDLRVEAKLRAFERQVIAELRMLQKIELMAKQMRSSAAAQNGDSPYRISYPLSRTPIHKITRADIEQALRDDYVRRLVNKEAQRRARNSGINTQKANALKRQAKSQDQTLSKEDIVQIMAYAYRMANEQMESEKGKQDKVYAAYRTEQNPMMMQQRQWSEEQAKIQQNQQQIQQNPMMMQQRQWSEEQAKIQQNQQQIQQNPMMMQQRQWSEEQAKIQQNQQQIQQNPMMMQQRQWSEEQAKIQQNQQQIQQNPMMVQQRQWSEEQAKIQQNQQQIQQNPMMMQQRQWSEEQAKIQHDQQMAQQMAQQGLMMTEQRQRQWSEDQAKIQQAQQMAQQTPMMMPQMQQRQWTEDPQMVQQMQQRQWAEDQTRMQMAQQNPMMQQQRQMAENPQMMQQRQWSEEQTKIEQAQQMAQQNQMMMQQMQQRQWSEDQAQIQQQQRQMMQQTPMMMKERQWAEENPQSVQQQGPMMMQQQMPSMMQREVEDEDNKAEDDLVGEAGPQMPENEGTARHKVDALGVGGNKRKKSKSKSAPPTVINYYYAAPQRPVVQSYGTSYGGGGYGSNAYGVPRPVNSYQSQGYRAAVGNDEVDEMLRQHQTMARTINPKQPGEVGGSESQKSNSNPPTTLTPAPQEQPQEHRVHKSPSSAPSETEIENAPSSDPQVGSIFTYGEGLLHPFMGLLPVERPDDPWNQKPYDPHHPLYTGGGSYDAYLRDGRHRRDTHIMGQGTQHGILTPGMLERLLRIKMDFQRRFPHLYKGMLNHHTNLTRVEVQPPVLGKISKPKTKTKPKNEDEPVFELGAAERSLFEDETNDSLEKDPEPEPDEEDDRDVEEPSESSEPRGFSSKKSRDENDIDYFNFDDDDVDD

Target ORF information:

RefSeq Version	NM_001258641.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster defective chorion 1 (dec-1), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258641.1

ORF Insert Sequence:

ATGAGATTGT TTAGCCTTCT GCCGCTCCTG GCGCTTCTTG TCGTCCAGGC TGCCGGACAA 
AGTGAAGTAA CCTCTGATGA TCCTGCAACG GATGCTGGAT CAACGACCAA TTCCACCACG 
GACACCAAAC CAAGGATTCC CAGTCAGGAT GAGATGGACG CATTTTACAT GATGTTCCCG 
GCGTTGGGCA GCCTGCTCAA GTGGGGCAGC CTTTTCCCAG CCTACTCGAT TCTGGGCGCC 
ATTCCCGACA ATCTACAGCC TACAGCGGCA GCCTCCAAGG TGGTCCTTGT CCTGGCCGAT 
GATGCGACGG CCAAGACTCG AGTTGCCCGT CAAAATCCGC CACCAAATCC ACTTGGTCAG 
CTAATGAATT GGCCCGCTCT GCCGCAGGAC TTCCAACTGC CTTCCATGGA TCTGGGACCG 
CAAGTGGGCT CGTTTTTGGC CCAACTGCCT GCTATGCCTA CCGTGCCTGG TCTTCTGGGT 
GCCGCTGCTC CAGTTCCTGC TCCAGCTCCT GCTCCCGCTG CTGCTCCTCC TCCGGCGCCA 
GCTCCAGCTG CAGATCCTCC GGCAGCACCT GTTCCAGATG CACCCCAACC AGCCATACTG 
GGACAAGCCG CTCTGCAGAA CGCTTTCACC TTCTTTAACC CGGCTAACTT TGATGCCTCC 
AGTCTTCTGG GCCAGAGTGT ACCCACATTT GCTCCTCCAA ATCTTGATTT CGTCGCTCAA 
ATGCAAAGGC AATTCTTCCC AGGAATGACA CCGGCACAAC CTGCTGCCGC TGGCACGGAT 
GCCCAGGCCT CCGACATTTC CGAGGTAAGG GTACGTCCTG AGGATCCGTA TTCGCAAGAG 
GCACAGATGA AGATCAAATC GGCACTTGAA ATGGAGCAGG AGAGGCAACA ACAGGCTCAG 
GTCAAGGATC AGGAGCAAGT GCCTCTCCTC TGGTTCCGAA TGCCCACAAC TCAGAATCAG 
GATGCGACTG AAGAAAAGAC TCTGGAGGAT CTGCGGGTCG AGGCCAAATT GAGGGCATTC 
GAGCGCCAGG TGATAGCCGA ATTGAGAATG CTGCAGAAGA TCGAACTTAT GGCTAAGCAG 
ATGAGGTCCA GCGCCGCAGC TCAAAACGGA GATTCACCCT ACAGAATCAG TTATCCACTG 
AGTCGGACAC CCATTCACAA GATCACCCGT GCTGATATTG AGCAAGCCCT TCGGGATGAT 
TATGTCCGCC GATTGGTCAA CAAGGAAGCG CAACGAAGGG CTAGAAATTC CGGCATAAAC 
ACCCAGAAGG CAAATGCCTT GAAGCGACAG GCCAAATCCC AGGATCAGAC TCTGTCCAAG 
GAGGATATCG TCCAGATTAT GGCGTATGCC TATCGCATGG CCAACGAACA GATGGAGAGC 
GAGAAGGGCA AGCAGGACAA GGTTTACGCG GCCTACAGGA CGGAACAGAA TCCAATGATG 
ATGCAGCAAA GACAATGGTC GGAGGAGCAG GCCAAGATCC AACAGAATCA ACAGCAGATC 
CAGCAAAATC CAATGATGAT GCAGCAACGA CAATGGTCGG AGGAGCAGGC CAAGATCCAA 
CAGAATCAAC AGCAGATCCA GCAAAATCCA ATGATGATGC AGCAACGACA ATGGTCGGAG 
GAGCAGGCCA AGATCCAACA GAATCAACAG CAGATCCAGC AAAATCCAAT GATGATGCAG 
CAAAGACAAT GGTCGGAGGA GCAGGCTAAG ATCCAACAGA ATCAACAGCA GATCCAGCAA 
AATCCAATGA TGGTGCAGCA AAGACAATGG TCGGAGGAGC AGGCCAAGAT CCAACAGAAT 
CAACAGCAGA TCCAGCAAAA TCCAATGATG ATGCAGCAAA GACAATGGTC GGAGGAGCAG 
GCCAAGATTC AGCATGATCA ACAGATGGCA CAACAGATGG CACAGCAAGG TCTCATGATG 
ACGGAGCAGA GGCAAAGGCA GTGGTCAGAA GATCAGGCCA AAATTCAGCA GGCTCAACAG 
ATGGCCCAAC AGACACCCAT GATGATGCCA CAGATGCAAC AAAGGCAGTG GACAGAGGAT 
CCCCAAATGG TTCAGCAGAT GCAACAGAGG CAGTGGGCAG AAGATCAGAC CAGGATGCAA 
ATGGCCCAAC AGAATCCAAT GATGCAACAG CAGCGACAGA TGGCAGAGAA TCCGCAAATG 
ATGCAGCAAA GGCAATGGTC CGAGGAGCAG ACCAAAATCG AACAGGCTCA GCAGATGGCA 
CAACAGAATC AAATGATGAT GCAGCAGATG CAGCAAAGAC AATGGTCGGA GGATCAGGCC 
CAGATTCAAC AGCAGCAGAG ACAGATGATG CAGCAGACCC CCATGATGAT GAAGGAGCGC 
CAGTGGGCGG AGGAGAACCC CCAGTCTGTC CAGCAGCAAG GGCCCATGAT GATGCAGCAG 
CAGATGCCAT CGATGATGCA GCGTGAAGTA GAGGACGAAG ATAATAAGGC AGAGGATGAC 
TTAGTTGGCG AGGCGGGACC CCAAATGCCG GAGAACGAAG GCACCGCCAG GCACAAAGTC 
GATGCCCTGG GAGTTGGCGG CAACAAGCGC AAGAAGTCCA AGTCCAAGTC GGCGCCGCCA 
ACGGTAATTA ACTATTACTA TGCGGCACCC CAGCGTCCGG TGGTTCAGAG TTACGGAACA 
AGCTACGGCG GAGGTGGCTA TGGATCGAAT GCCTATGGTG TTCCTCGTCC AGTCAATTCG 
TATCAAAGTC AAGGCTACAG GGCTGCCGTG GGTAACGATG AGGTAGATGA GATGTTGCGC 
CAGCACCAGA CAATGGCCAG GACAATAAAC CCGAAGCAAC CAGGCGAAGT CGGTGGATCG 
GAGAGCCAGA AGAGCAACTC TAATCCTCCA ACGACGTTGA CACCAGCTCC ACAAGAGCAA 
CCGCAAGAGC ATCGAGTCCA CAAAAGTCCA TCATCAGCAC CATCCGAAAC CGAAATCGAA 
AACGCACCAT CATCTGACCC CCAAGTGGGT TCCATTTTCA CCTACGGCGA GGGATTGCTG 
CATCCGTTTA TGGGTCTGCT GCCGGTGGAG AGGCCCGATG ATCCGTGGAA CCAGAAGCCA 
TACGATCCTC ACCATCCTCT CTACACGGGC GGTGGGAGCT ACGATGCCTA TCTGAGGGAC 
GGTCGCCATC GAAGGGACAC GCACATCATG GGTCAAGGTA CGCAGCATGG CATCCTGACA 
CCCGGCATGT TGGAGAGACT TCTTCGGATC AAGATGGATT TCCAGCGTAG ATTTCCCCAT 
TTGTACAAGG GTATGCTGAA TCACCATACG AATCTTACCC GTGTAGAGGT ACAGCCTCCG 
GTTCTGGGCA AGATTTCTAA GCCGAAAACT AAGACCAAGC CCAAGAATGA GGACGAACCC 
GTCTTCGAAT TGGGAGCCGC TGAGCGTAGC CTGTTCGAGG ACGAGACCAA CGATTCTTTA 
GAGAAAGATC CCGAACCAGA ACCGGATGAG GAGGATGACC GGGATGTGGA AGAGCCCAGC 
GAGAGCAGTG AACCTAGGGG ATTCAGCAGC AAAAAAAGCC GGGATGAGAA CGACATCGAT 
TACTTCAATT TCGATGACGA TGATGTTGAT GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dec-1 ( NM_001258640.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245569.1 Drosophila melanogaster defective chorion 1 (dec-1), transcript variant G, mRNA.

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CloneID	OFa03017
Clone ID Related Accession (Same CDS sequence)	NM_001258640.2
Accession Version	NM_001258640.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	defective chorion 1, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258640.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGATTGT TTAGCCTTCT GCCGCTCCTG GCGCTTCTTG TCGTCCAGGC TGCCGGACAA 
AGTGAAGTAA CCTCTGATGA TCCTGCAACG GATGCTGGAT CAACGACCAA TTCCACCACG 
GACACCAAAC CAAGGATTCC CAGTCAGGAT GAGATGGACG CATTTTACAT GATGTTCCCG 
GCGTTGGGCA GCCTGCTCAA GTGGGGCAGC CTTTTCCCAG CCTACTCGAT TCTGGGCGCC 
ATTCCCGACA ATCTACAGCC TACAGCGGCA GCCTCCAAGG TGGTCCTTGT CCTGGCCGAT 
GATGCGACGG CCAAGACTCG AGTTGCCCGT CAAAATCCGC CACCAAATCC ACTTGGTCAG 
CTAATGAATT GGCCCGCTCT GCCGCAGGAC TTCCAACTGC CTTCCATGGA TCTGGGACCG 
CAAGTGGGCT CGTTTTTGGC CCAACTGCCT GCTATGCCTA CCGTGCCTGG TCTTCTGGGT 
GCCGCTGCTC CAGTTCCTGC TCCAGCTCCT GCTCCCGCTG CTGCTCCTCC TCCGGCGCCA 
GCTCCAGCTG CAGATCCTCC GGCAGCACCT GTTCCAGATG CACCCCAACC AGCCATACTG 
GGACAAGCCG CTCTGCAGAA CGCTTTCACC TTCTTTAACC CGGCTAACTT TGATGCCTCC 
AGTCTTCTGG GCCAGAGTGT ACCCACATTT GCTCCTCCAA ATCTTGATTT CGTCGCTCAA 
ATGCAAAGGC AATTCTTCCC AGGAATGACA CCGGCACAAC CTGCTGCCGC TGGCACGGAT 
GCCCAGGCCT CCGACATTTC CGAGGTAAGG GTACGTCCTG AGGATCCGTA TTCGCAAGAG 
GCACAGATGA AGATCAAATC GGCACTTGAA ATGGAGCAGG AGAGGCAACA ACAGGCTCAG 
GTCAAGGATC AGGAGCAAGT GCCTCTCCTC TGGTTCCGAA TGCCCACAAC TCAGAATCAG 
GATGCGACTG AAGAAAAGAC TCTGGAGGAT CTGCGGGTCG AGGCCAAATT GAGGGCATTC 
GAGCGCCAGG TGATAGCCGA ATTGAGAATG CTGCAGAAGA TCGAACTTAT GGCTAAGCAG 
ATGAGGTCCA GCGCCGCAGC TCAAAACGGA GATTCACCCT ACAGAATCAG TTATCCACTG 
AGTCGGACAC CCATTCACAA GATCACCCGT GCTGATATTG AGCAAGCCCT TCGGGATGAT 
TATGTCCGCC GATTGGTCAA CAAGGAAGCG CAACGAAGGG CTAGAAATTC CGGCATAAAC 
ACCCAGAAGG CAAATGCCTT GAAGCGACAG GCCAAATCCC AGGATCAGAC TCTGTCCAAG 
GAGGATATCG TCCAGATTAT GGCGTATGCC TATCGCATGG CCAACGAACA GATGGAGAGC 
GAGAAGGGCA AGCAGGACAA GGTTTACGCG GCCTACAGGA CGGAACAGAA TCCAATGATG 
ATGCAGCAAA GACAATGGTC GGAGGAGCAG GCCAAGATCC AACAGAATCA ACAGCAGATC 
CAGCAAAATC CAATGATGAT GCAGCAACGA CAATGGTCGG AGGAGCAGGC CAAGATCCAA 
CAGAATCAAC AGCAGATCCA GCAAAATCCA ATGATGATGC AGCAACGACA ATGGTCGGAG 
GAGCAGGCCA AGATCCAACA GAATCAACAG CAGATCCAGC AAAATCCAAT GATGATGCAG 
CAAAGACAAT GGTCGGAGGA GCAGGCTAAG ATCCAACAGA ATCAACAGCA GATCCAGCAA 
AATCCAATGA TGGTGCAGCA AAGACAATGG TCGGAGGAGC AGGCCAAGAT CCAACAGAAT 
CAACAGCAGA TCCAGCAAAA TCCAATGATG ATGCAGCAAA GACAATGGTC GGAGGAGCAG 
GCCAAGATTC AGCATGATCA ACAGATGGCA CAACAGATGG CACAGCAAGG TCTCATGATG 
ACGGAGCAGA GGCAAAGGCA GTGGTCAGAA GATCAGGCCA AAATTCAGCA GGCTCAACAG 
ATGGCCCAAC AGACACCCAT GATGATGCCA CAGATGCAAC AAAGGCAGTG GACAGAGGAT 
CCCCAAATGG TTCAGCAGAT GCAACAGAGG CAGTGGGCAG AAGATCAGAC CAGGATGCAA 
ATGGCCCAAC AGAATCCAAT GATGCAACAG CAGCGACAGA TGGCAGAGAA TCCGCAAATG 
ATGCAGCAAA GGCAATGGTC CGAGGAGCAG ACCAAAATCG AACAGGCTCA GCAGATGGCA 
CAACAGAATC AAATGATGAT GCAGCAGATG CAGCAAAGAC AATGGTCGGA GGATCAGGCC 
CAGATTCAAC AGCAGCAGAG ACAGATGATG CAGCAGACCC CCATGATGAT GAAGGAGCGC 
CAGTGGGCGG AGGAGAACCC CCAGTCTGTC CAGCAGCAAG GGCCCATGAT GATGCAGCAG 
CAGATGCCAT CGATGATGCA GCGTGAAGTA GAGGACGAAG ATAATAAGGC AGAGGATGAC 
TTAGTTGGCG AGGCGGGACC CCAAATGCCG GAGAACGAAG GCACCGCCAG GCACAAAGTC 
GATGCCCTGG GAGTTGGCGG CAACAAGCGC AAGAAGTCCA AGTCCAAGTC GGCGCCGCCA 
ACGGTAATTA ACTATTACTA TGCGGCACCC CAGCGTCCGG TGGTTCAGAG TTACGGAACA 
AGCTACGGCG GAGGTGGCTA TGGATCGAAT GCCTATGGTG TTCCTCGTCC AGTCAATTCG 
TATCAAAGTC AAGGCTACAG GGCTGCCGTG GGTAACGATG AGGTAGATGA GATGTTGCGC 
CAGCACCAGA CAATGGCCAG GACAATAAAC CCGAAGCAAC CAGGCGAAGT CGGTGGATCG 
GAGAGCCAGA AGAGCAACTC TAATCCTCCA ACGACGTTGA CACCAGCTCC ACAAGAGCAA 
CCGCAAGAGC ATCGAGTCCA CAAAAGGTTA GCACATTTCC ACAGGTTTGG GCGAGAGGCA 
GGGTTAAATG CGACCACATC CAAGGGTTGC GGATGTGGCA GGTTGGATTG TCTGTGCGGC 
AGGAGTTGTC GATGCGGAAG AAGAGGCTTG GAATCTAGAG TGGTTTCTAG TAGAACTTCG 
GGAACTTGCC AGTGCAAAGC CAGTCACCGG AACAAGCGTA GCGTTGAATA TGGAACCTTG 
GAGACCATCG ATGAGGGTTC TCTTAACGAA CTTAGAAGAG AATATAAACT GGGACTAAAA 
GAGATCACGC TGAGTCCAGA TGAAGATCCC GCCGAGGCCC TGATGCGCTA CAATGCGGCC 
TCCATTCGCG AAGCTTTGGA GCGAGCCAGT ATGGAACCGC TGGAGATCGG TGGGGATCAG 
TATGAAGAGG ATGCGCAACA GGAGCCCATG GAGGAGGAGC AACTACAGCA TGATCCCAAC 
ACAGAGCCCC AGTACAATCA TAAGGACTTT GTGCGGCTAA CCACTTCCAC AGCATCTCCG 
ATCACAAGTA CCACAGAAGC TGCCACTCCA ACAGGCAGTG ACTCTACTTC AGAAGCAACT 
GTGACACCTG AAGTCACCAC CACCACTAGC ACATCCACAA CAACAACAAC CGAAAGCACC 
AAAGATGAGG GACTGGACAT GCAGCAAGAC TCACAGGCTG AGGCTGAGAG TTCTCATGTC 
ACCAAGTCGA TATCGAAGCA GGAGGCCGAG ATTCATCAGC TGCACAGTAT TGTGGAGGAG 
CTGAAGAATG AGATCCTTAA ACTGAATTTG CGTTGCAGCA CGATAATATC AAACAATGTG 
GCCAAAGAAC CGGTCACAGA GAAGAACCCA CCCGTGGTGG AGGAACCATC CAAGCAGGAG 
GATAAGCCAA AGGTGGAGGA GAAAGTGATT GCCGAAGAGC AAGCCCCAGT GGAGCAGGAG 
GAAGAGCTGG AAGAGGACGA GGATAGTACC TCTATTAGTA CGACCACAGA GACTCCTTCA 
CCAAGTGGTA GTTACTCAAC AAAACCTGGA CTTTCCCTTG GATCGCCTAG AGTGGATGAA 
CAAAGTGGCT CGTCCAATAA ACTGGACTAC GACGACGATA ACAATTGGCA ACGCATTCTG 
GCCAATCGTG GCTATGACAC GGACTACCTA ACCAAATCCC ATGAGCGTCA GTTTGCCCAG 
GGTCAAAACC TGGAGATGCC CAAGAACTGC AATTACGATG GAAATGGCAG CCAAGAGTAT 
GGCCCCTATC CGGAGTTCCA AGCAGATGAA CCCAGCACGG ATACGGAGGG CAAGGCTAAA 
AGGGCTTTAA GTGTGAAACA ACAGGCGCAG CTGCTCAATG CGGCCCTAAA TGATAGTGGA 
AGCGATTCGA GCGATGGCAC CACCACCACC ACCACACCCT CTCCGTACGC GATGAGGGGA 
AAGTTTGTGA GACGGAGGAG CACGGCAAGA AGGGTTCCCA TTCCAAAGAT AGGAAAGGCG 
AGCGACGAGG TATGGGTACG ATCTCCTCGT CAAGCGAAAA TGCCCCAACG ACCCAAGAAG 
TCCATGTCCA AGCCGAAAAA GCAGAGCTCG CAGGTGACTA CGCAGGCCAC CGTGAGCAGC 
ACTAAGCTGG ACAGTTTGGT GGATGTGCTC AAGGATCTGG TGCGCCTGCA AATTCAAAAG 
GAGAAAAAAT CCAGCCTATT AAGAACCCAG AGCAATAACC TTTCGAAAAC CAAACCCAAA 
TCTATTAAGC CCGTCAAAGT CATCAAGCGA AAAAGGTTGC GCAGGAGGCA ACACAAGTCT 
ATTGCCACGA CCATCAGGAG TCCCATTCAA ACGAAAGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245569.1
CDS	76..4797
Translation	MRLFSLLPLLALLVVQAAGQSEVTSDDPATDAGSTTNSTTDTKPRIPSQDEMDAFYMMFPALGSLLKWGSLFPAYSILGAIPDNLQPTAAASKVVLVLADDATAKTRVARQNPPPNPLGQLMNWPALPQDFQLPSMDLGPQVGSFLAQLPAMPTVPGLLGAAAPVPAPAPAPAAAPPPAPAPAADPPAAPVPDAPQPAILGQAALQNAFTFFNPANFDASSLLGQSVPTFAPPNLDFVAQMQRQFFPGMTPAQPAAAGTDAQASDISEVRVRPEDPYSQEAQMKIKSALEMEQERQQQAQVKDQEQVPLLWFRMPTTQNQDATEEKTLEDLRVEAKLRAFERQVIAELRMLQKIELMAKQMRSSAAAQNGDSPYRISYPLSRTPIHKITRADIEQALRDDYVRRLVNKEAQRRARNSGINTQKANALKRQAKSQDQTLSKEDIVQIMAYAYRMANEQMESEKGKQDKVYAAYRTEQNPMMMQQRQWSEEQAKIQQNQQQIQQNPMMMQQRQWSEEQAKIQQNQQQIQQNPMMMQQRQWSEEQAKIQQNQQQIQQNPMMMQQRQWSEEQAKIQQNQQQIQQNPMMVQQRQWSEEQAKIQQNQQQIQQNPMMMQQRQWSEEQAKIQHDQQMAQQMAQQGLMMTEQRQRQWSEDQAKIQQAQQMAQQTPMMMPQMQQRQWTEDPQMVQQMQQRQWAEDQTRMQMAQQNPMMQQQRQMAENPQMMQQRQWSEEQTKIEQAQQMAQQNQMMMQQMQQRQWSEDQAQIQQQQRQMMQQTPMMMKERQWAEENPQSVQQQGPMMMQQQMPSMMQREVEDEDNKAEDDLVGEAGPQMPENEGTARHKVDALGVGGNKRKKSKSKSAPPTVINYYYAAPQRPVVQSYGTSYGGGGYGSNAYGVPRPVNSYQSQGYRAAVGNDEVDEMLRQHQTMARTINPKQPGEVGGSESQKSNSNPPTTLTPAPQEQPQEHRVHKRLAHFHRFGREAGLNATTSKGCGCGRLDCLCGRSCRCGRRGLESRVVSSRTSGTCQCKASHRNKRSVEYGTLETIDEGSLNELRREYKLGLKEITLSPDEDPAEALMRYNAASIREALERASMEPLEIGGDQYEEDAQQEPMEEEQLQHDPNTEPQYNHKDFVRLTTSTASPITSTTEAATPTGSDSTSEATVTPEVTTTTSTSTTTTTESTKDEGLDMQQDSQAEAESSHVTKSISKQEAEIHQLHSIVEELKNEILKLNLRCSTIISNNVAKEPVTEKNPPVVEEPSKQEDKPKVEEKVIAEEQAPVEQEEELEEDEDSTSISTTTETPSPSGSYSTKPGLSLGSPRVDEQSGSSNKLDYDDDNNWQRILANRGYDTDYLTKSHERQFAQGQNLEMPKNCNYDGNGSQEYGPYPEFQADEPSTDTEGKAKRALSVKQQAQLLNAALNDSGSDSSDGTTTTTTPSPYAMRGKFVRRRSTARRVPIPKIGKASDEVWVRSPRQAKMPQRPKKSMSKPKKQSSQVTTQATVSSTKLDSLVDVLKDLVRLQIQKEKKSSLLRTQSNNLSKTKPKSIKPVKVIKRKRLRRRQHKSIATTIRSPIQTKA

Target ORF information:

RefSeq Version	NM_001258640.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster defective chorion 1 (dec-1), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258640.2

ORF Insert Sequence:

ATGAGATTGT TTAGCCTTCT GCCGCTCCTG GCGCTTCTTG TCGTCCAGGC TGCCGGACAA 
AGTGAAGTAA CCTCTGATGA TCCTGCAACG GATGCTGGAT CAACGACCAA TTCCACCACG 
GACACCAAAC CAAGGATTCC CAGTCAGGAT GAGATGGACG CATTTTACAT GATGTTCCCG 
GCGTTGGGCA GCCTGCTCAA GTGGGGCAGC CTTTTCCCAG CCTACTCGAT TCTGGGCGCC 
ATTCCCGACA ATCTACAGCC TACAGCGGCA GCCTCCAAGG TGGTCCTTGT CCTGGCCGAT 
GATGCGACGG CCAAGACTCG AGTTGCCCGT CAAAATCCGC CACCAAATCC ACTTGGTCAG 
CTAATGAATT GGCCCGCTCT GCCGCAGGAC TTCCAACTGC CTTCCATGGA TCTGGGACCG 
CAAGTGGGCT CGTTTTTGGC CCAACTGCCT GCTATGCCTA CCGTGCCTGG TCTTCTGGGT 
GCCGCTGCTC CAGTTCCTGC TCCAGCTCCT GCTCCCGCTG CTGCTCCTCC TCCGGCGCCA 
GCTCCAGCTG CAGATCCTCC GGCAGCACCT GTTCCAGATG CACCCCAACC AGCCATACTG 
GGACAAGCCG CTCTGCAGAA CGCTTTCACC TTCTTTAACC CGGCTAACTT TGATGCCTCC 
AGTCTTCTGG GCCAGAGTGT ACCCACATTT GCTCCTCCAA ATCTTGATTT CGTCGCTCAA 
ATGCAAAGGC AATTCTTCCC AGGAATGACA CCGGCACAAC CTGCTGCCGC TGGCACGGAT 
GCCCAGGCCT CCGACATTTC CGAGGTAAGG GTACGTCCTG AGGATCCGTA TTCGCAAGAG 
GCACAGATGA AGATCAAATC GGCACTTGAA ATGGAGCAGG AGAGGCAACA ACAGGCTCAG 
GTCAAGGATC AGGAGCAAGT GCCTCTCCTC TGGTTCCGAA TGCCCACAAC TCAGAATCAG 
GATGCGACTG AAGAAAAGAC TCTGGAGGAT CTGCGGGTCG AGGCCAAATT GAGGGCATTC 
GAGCGCCAGG TGATAGCCGA ATTGAGAATG CTGCAGAAGA TCGAACTTAT GGCTAAGCAG 
ATGAGGTCCA GCGCCGCAGC TCAAAACGGA GATTCACCCT ACAGAATCAG TTATCCACTG 
AGTCGGACAC CCATTCACAA GATCACCCGT GCTGATATTG AGCAAGCCCT TCGGGATGAT 
TATGTCCGCC GATTGGTCAA CAAGGAAGCG CAACGAAGGG CTAGAAATTC CGGCATAAAC 
ACCCAGAAGG CAAATGCCTT GAAGCGACAG GCCAAATCCC AGGATCAGAC TCTGTCCAAG 
GAGGATATCG TCCAGATTAT GGCGTATGCC TATCGCATGG CCAACGAACA GATGGAGAGC 
GAGAAGGGCA AGCAGGACAA GGTTTACGCG GCCTACAGGA CGGAACAGAA TCCAATGATG 
ATGCAGCAAA GACAATGGTC GGAGGAGCAG GCCAAGATCC AACAGAATCA ACAGCAGATC 
CAGCAAAATC CAATGATGAT GCAGCAACGA CAATGGTCGG AGGAGCAGGC CAAGATCCAA 
CAGAATCAAC AGCAGATCCA GCAAAATCCA ATGATGATGC AGCAACGACA ATGGTCGGAG 
GAGCAGGCCA AGATCCAACA GAATCAACAG CAGATCCAGC AAAATCCAAT GATGATGCAG 
CAAAGACAAT GGTCGGAGGA GCAGGCTAAG ATCCAACAGA ATCAACAGCA GATCCAGCAA 
AATCCAATGA TGGTGCAGCA AAGACAATGG TCGGAGGAGC AGGCCAAGAT CCAACAGAAT 
CAACAGCAGA TCCAGCAAAA TCCAATGATG ATGCAGCAAA GACAATGGTC GGAGGAGCAG 
GCCAAGATTC AGCATGATCA ACAGATGGCA CAACAGATGG CACAGCAAGG TCTCATGATG 
ACGGAGCAGA GGCAAAGGCA GTGGTCAGAA GATCAGGCCA AAATTCAGCA GGCTCAACAG 
ATGGCCCAAC AGACACCCAT GATGATGCCA CAGATGCAAC AAAGGCAGTG GACAGAGGAT 
CCCCAAATGG TTCAGCAGAT GCAACAGAGG CAGTGGGCAG AAGATCAGAC CAGGATGCAA 
ATGGCCCAAC AGAATCCAAT GATGCAACAG CAGCGACAGA TGGCAGAGAA TCCGCAAATG 
ATGCAGCAAA GGCAATGGTC CGAGGAGCAG ACCAAAATCG AACAGGCTCA GCAGATGGCA 
CAACAGAATC AAATGATGAT GCAGCAGATG CAGCAAAGAC AATGGTCGGA GGATCAGGCC 
CAGATTCAAC AGCAGCAGAG ACAGATGATG CAGCAGACCC CCATGATGAT GAAGGAGCGC 
CAGTGGGCGG AGGAGAACCC CCAGTCTGTC CAGCAGCAAG GGCCCATGAT GATGCAGCAG 
CAGATGCCAT CGATGATGCA GCGTGAAGTA GAGGACGAAG ATAATAAGGC AGAGGATGAC 
TTAGTTGGCG AGGCGGGACC CCAAATGCCG GAGAACGAAG GCACCGCCAG GCACAAAGTC 
GATGCCCTGG GAGTTGGCGG CAACAAGCGC AAGAAGTCCA AGTCCAAGTC GGCGCCGCCA 
ACGGTAATTA ACTATTACTA TGCGGCACCC CAGCGTCCGG TGGTTCAGAG TTACGGAACA 
AGCTACGGCG GAGGTGGCTA TGGATCGAAT GCCTATGGTG TTCCTCGTCC AGTCAATTCG 
TATCAAAGTC AAGGCTACAG GGCTGCCGTG GGTAACGATG AGGTAGATGA GATGTTGCGC 
CAGCACCAGA CAATGGCCAG GACAATAAAC CCGAAGCAAC CAGGCGAAGT CGGTGGATCG 
GAGAGCCAGA AGAGCAACTC TAATCCTCCA ACGACGTTGA CACCAGCTCC ACAAGAGCAA 
CCGCAAGAGC ATCGAGTCCA CAAAAGGTTA GCACATTTCC ACAGGTTTGG GCGAGAGGCA 
GGGTTAAATG CGACCACATC CAAGGGTTGC GGATGTGGCA GGTTGGATTG TCTGTGCGGC 
AGGAGTTGTC GATGCGGAAG AAGAGGCTTG GAATCTAGAG TGGTTTCTAG TAGAACTTCG 
GGAACTTGCC AGTGCAAAGC CAGTCACCGG AACAAGCGTA GCGTTGAATA TGGAACCTTG 
GAGACCATCG ATGAGGGTTC TCTTAACGAA CTTAGAAGAG AATATAAACT GGGACTAAAA 
GAGATCACGC TGAGTCCAGA TGAAGATCCC GCCGAGGCCC TGATGCGCTA CAATGCGGCC 
TCCATTCGCG AAGCTTTGGA GCGAGCCAGT ATGGAACCGC TGGAGATCGG TGGGGATCAG 
TATGAAGAGG ATGCGCAACA GGAGCCCATG GAGGAGGAGC AACTACAGCA TGATCCCAAC 
ACAGAGCCCC AGTACAATCA TAAGGACTTT GTGCGGCTAA CCACTTCCAC AGCATCTCCG 
ATCACAAGTA CCACAGAAGC TGCCACTCCA ACAGGCAGTG ACTCTACTTC AGAAGCAACT 
GTGACACCTG AAGTCACCAC CACCACTAGC ACATCCACAA CAACAACAAC CGAAAGCACC 
AAAGATGAGG GACTGGACAT GCAGCAAGAC TCACAGGCTG AGGCTGAGAG TTCTCATGTC 
ACCAAGTCGA TATCGAAGCA GGAGGCCGAG ATTCATCAGC TGCACAGTAT TGTGGAGGAG 
CTGAAGAATG AGATCCTTAA ACTGAATTTG CGTTGCAGCA CGATAATATC AAACAATGTG 
GCCAAAGAAC CGGTCACAGA GAAGAACCCA CCCGTGGTGG AGGAACCATC CAAGCAGGAG 
GATAAGCCAA AGGTGGAGGA GAAAGTGATT GCCGAAGAGC AAGCCCCAGT GGAGCAGGAG 
GAAGAGCTGG AAGAGGACGA GGATAGTACC TCTATTAGTA CGACCACAGA GACTCCTTCA 
CCAAGTGGTA GTTACTCAAC AAAACCTGGA CTTTCCCTTG GATCGCCTAG AGTGGATGAA 
CAAAGTGGCT CGTCCAATAA ACTGGACTAC GACGACGATA ACAATTGGCA ACGCATTCTG 
GCCAATCGTG GCTATGACAC GGACTACCTA ACCAAATCCC ATGAGCGTCA GTTTGCCCAG 
GGTCAAAACC TGGAGATGCC CAAGAACTGC AATTACGATG GAAATGGCAG CCAAGAGTAT 
GGCCCCTATC CGGAGTTCCA AGCAGATGAA CCCAGCACGG ATACGGAGGG CAAGGCTAAA 
AGGGCTTTAA GTGTGAAACA ACAGGCGCAG CTGCTCAATG CGGCCCTAAA TGATAGTGGA 
AGCGATTCGA GCGATGGCAC CACCACCACC ACCACACCCT CTCCGTACGC GATGAGGGGA 
AAGTTTGTGA GACGGAGGAG CACGGCAAGA AGGGTTCCCA TTCCAAAGAT AGGAAAGGCG 
AGCGACGAGG TATGGGTACG ATCTCCTCGT CAAGCGAAAA TGCCCCAACG ACCCAAGAAG 
TCCATGTCCA AGCCGAAAAA GCAGAGCTCG CAGGTGACTA CGCAGGCCAC CGTGAGCAGC 
ACTAAGCTGG ACAGTTTGGT GGATGTGCTC AAGGATCTGG TGCGCCTGCA AATTCAAAAG 
GAGAAAAAAT CCAGCCTATT AAGAACCCAG AGCAATAACC TTTCGAAAAC CAAACCCAAA 
TCTATTAAGC CCGTCAAAGT CATCAAGCGA AAAAGGTTGC GCAGGAGGCA ACACAAGTCT 
ATTGCCACGA CCATCAGGAG TCCCATTCAA ACGAAAGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dec-1 ( NM_001258642.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245571.1 Drosophila melanogaster defective chorion 1 (dec-1), transcript variant F, mRNA.

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CloneID	OFa03018
Clone ID Related Accession (Same CDS sequence)	NM_001258642.1
Accession Version	NM_001258642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	defective chorion 1, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGATTGT TTAGCCTTCT GCCGCTCCTG GCGCTTCTTG TCGTCCAGGC TGCCGGACAA 
AGTGAAGTAA CCTCTGATGA TCCTGCAACG GATGCTGGAT CAACGACCAA TTCCACCACG 
GACACCAAAC CAAGGATTCC CAGTCAGGAT GAGATCCTTG GCCAGATGCC GTCCATTAAT 
CCCATCCGCA CTGGCAATCC CCAGATGGAC GCATTTTACA TGATGTTCCC GGCGTTGGGC 
AGCCTGCTCA AGTGGGGCAG CCTTTTCCCA GCCTACTCGA TTCTGGGCGC CATTCCCGAC 
AATCTACAGC CTACAGCGGC AGCCTCCAAG GTGGTCCTTG TCCTGGCCGA TGATGCGACG 
GCCAAGACTC GAGTTGCCCG TCAAAATCCG CCACCAAATC CACTTGGTCA GCTAATGAAT 
TGGCCCGCTC TGCCGCAGGA CTTCCAACTG CCTTCCATGG ATCTGGGACC GCAAGTGGGC 
TCGTTTTTGG CCCAACTGCC TGCTATGCCT ACCGTGCCTG GTCTTCTGGG TGCCGCTGCT 
CCAGTTCCTG CTCCAGCTCC TGCTCCCGCT GCTGCTCCTC CTCCGGCGCC AGCTCCAGCT 
GCAGATCCTC CGGCAGCACC TGTTCCAGAT GCACCCCAAC CAGCCATACT GGGACAAGCC 
GCTCTGCAGA ACGCTTTCAC CTTCTTTAAC CCGGCTAACT TTGATGCCTC CAGTCTTCTG 
GGCCAGAGTG TACCCACATT TGCTCCTCCA AATCTTGATT TCGTCGCTCA AATGCAAAGG 
CAATTCTTCC CAGGAATGAC ACCGGCACAA CCTGCTGCCG CTGGCACGGA TGCCCAGGCC 
TCCGACATTT CCGAGGTAAG GGTACGTCCT GAGGATCCGT ATTCGCAAGA GGCACAGATG 
AAGATCAAAT CGGCACTTGA AATGGAGCAG GAGAGGCAAC AACAGGCTCA GGTCAAGGAT 
CAGGAGCAAG TGCCTCTCCT CTGGTTCCGA ATGCCCACAA CTCAGAATCA GGATGCGACT 
GAAGAAAAGA CTCTGGAGGA TCTGCGGGTC GAGGCCAAAT TGAGGGCATT CGAGCGCCAG 
GTGATAGCCG AATTGAGAAT GCTGCAGAAG ATCGAACTTA TGGCTAAGCA GATGAGGTCC 
AGCGCCGCAG CTCAAAACGG AGATTCACCC TACAGAATCA GTTATCCACT GAGTCGGACA 
CCCATTCACA AGATCACCCG TGCTGATATT GAGCAAGCCC TTCGGGATGA TTATGTCCGC 
CGATTGGTCA ACAAGGAAGC GCAACGAAGG GCTAGAAATT CCGGCATAAA CACCCAGAAG 
GCAAATGCCT TGAAGCGACA GGCCAAATCC CAGGATCAGA CTCTGTCCAA GGAGGATATC 
GTCCAGATTA TGGCGTATGC CTATCGCATG GCCAACGAAC AGATGGAGAG CGAGAAGGGC 
AAGCAGGACA AGGTTTACGC GGCCTACAGG ACGGAACAGA ATCCAATGAT GATGCAGCAA 
AGACAATGGT CGGAGGAGCA GGCCAAGATC CAACAGAATC AACAGCAGAT CCAGCAAAAT 
CCAATGATGA TGCAGCAACG ACAATGGTCG GAGGAGCAGG CCAAGATCCA ACAGAATCAA 
CAGCAGATCC AGCAAAATCC AATGATGATG CAGCAACGAC AATGGTCGGA GGAGCAGGCC 
AAGATCCAAC AGAATCAACA GCAGATCCAG CAAAATCCAA TGATGATGCA GCAAAGACAA 
TGGTCGGAGG AGCAGGCTAA GATCCAACAG AATCAACAGC AGATCCAGCA AAATCCAATG 
ATGGTGCAGC AAAGACAATG GTCGGAGGAG CAGGCCAAGA TCCAACAGAA TCAACAGCAG 
ATCCAGCAAA ATCCAATGAT GATGCAGCAA AGACAATGGT CGGAGGAGCA GGCCAAGATT 
CAGCATGATC AACAGATGGC ACAACAGATG GCACAGCAAG GTCTCATGAT GACGGAGCAG 
AGGCAAAGGC AGTGGTCAGA AGATCAGGCC AAAATTCAGC AGGCTCAACA GATGGCCCAA 
CAGACACCCA TGATGATGCC ACAGATGCAA CAAAGGCAGT GGACAGAGGA TCCCCAAATG 
GTTCAGCAGA TGCAACAGAG GCAGTGGGCA GAAGATCAGA CCAGGATGCA AATGGCCCAA 
CAGAATCCAA TGATGCAACA GCAGCGACAG ATGGCAGAGA ATCCGCAAAT GATGCAGCAA 
AGGCAATGGT CCGAGGAGCA GACCAAAATC GAACAGGCTC AGCAGATGGC ACAACAGAAT 
CAAATGATGA TGCAGCAGAT GCAGCAAAGA CAATGGTCGG AGGATCAGGC CCAGATTCAA 
CAGCAGCAGA GACAGATGAT GCAGCAGACC CCCATGATGA TGAAGGAGCG CCAGTGGGCG 
GAGGAGAACC CCCAGTCTGT CCAGCAGCAA GGGCCCATGA TGATGCAGCA GCAGATGCCA 
TCGATGATGC AGCGTGAAGT AGAGGACGAA GATAATAAGG CAGAGGATGA CTTAGTTGGC 
GAGGCGGGAC CCCAAATGCC GGAGAACGAA GGCACCGCCA GGCACAAAGT CGATGCCCTG 
GGAGTTGGCG GCAACAAGCG CAAGAAGTCC AAGTCCAAGT CGGCGCCGCC AACGGTAATT 
AACTATTACT ATGCGGCACC CCAGCGTCCG GTGGTTCAGA GTTACGGAAC AAGCTACGGC 
GGAGGTGGCT ATGGATCGAA TGCCTATGGT GTTCCTCGTC CAGTCAATTC GTATCAAAGT 
CAAGGCTACA GGGCTGCCGT GGGTAACGAT GAGGTAGATG AGATGTTGCG CCAGCACCAG 
ACAATGGCCA GGTCCATCAT CAGCACCATC CGAAACCGAA ATCGAAAACG CACCATCATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245571.1
CDS	76..2958
Translation	MRLFSLLPLLALLVVQAAGQSEVTSDDPATDAGSTTNSTTDTKPRIPSQDEILGQMPSINPIRTGNPQMDAFYMMFPALGSLLKWGSLFPAYSILGAIPDNLQPTAAASKVVLVLADDATAKTRVARQNPPPNPLGQLMNWPALPQDFQLPSMDLGPQVGSFLAQLPAMPTVPGLLGAAAPVPAPAPAPAAAPPPAPAPAADPPAAPVPDAPQPAILGQAALQNAFTFFNPANFDASSLLGQSVPTFAPPNLDFVAQMQRQFFPGMTPAQPAAAGTDAQASDISEVRVRPEDPYSQEAQMKIKSALEMEQERQQQAQVKDQEQVPLLWFRMPTTQNQDATEEKTLEDLRVEAKLRAFERQVIAELRMLQKIELMAKQMRSSAAAQNGDSPYRISYPLSRTPIHKITRADIEQALRDDYVRRLVNKEAQRRARNSGINTQKANALKRQAKSQDQTLSKEDIVQIMAYAYRMANEQMESEKGKQDKVYAAYRTEQNPMMMQQRQWSEEQAKIQQNQQQIQQNPMMMQQRQWSEEQAKIQQNQQQIQQNPMMMQQRQWSEEQAKIQQNQQQIQQNPMMMQQRQWSEEQAKIQQNQQQIQQNPMMVQQRQWSEEQAKIQQNQQQIQQNPMMMQQRQWSEEQAKIQHDQQMAQQMAQQGLMMTEQRQRQWSEDQAKIQQAQQMAQQTPMMMPQMQQRQWTEDPQMVQQMQQRQWAEDQTRMQMAQQNPMMQQQRQMAENPQMMQQRQWSEEQTKIEQAQQMAQQNQMMMQQMQQRQWSEDQAQIQQQQRQMMQQTPMMMKERQWAEENPQSVQQQGPMMMQQQMPSMMQREVEDEDNKAEDDLVGEAGPQMPENEGTARHKVDALGVGGNKRKKSKSKSAPPTVINYYYAAPQRPVVQSYGTSYGGGGYGSNAYGVPRPVNSYQSQGYRAAVGNDEVDEMLRQHQTMARSIISTIRNRNRKRTII

Target ORF information:

RefSeq Version	NM_001258642.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster defective chorion 1 (dec-1), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258642.1

ORF Insert Sequence:

ATGAGATTGT TTAGCCTTCT GCCGCTCCTG GCGCTTCTTG TCGTCCAGGC TGCCGGACAA 
AGTGAAGTAA CCTCTGATGA TCCTGCAACG GATGCTGGAT CAACGACCAA TTCCACCACG 
GACACCAAAC CAAGGATTCC CAGTCAGGAT GAGATCCTTG GCCAGATGCC GTCCATTAAT 
CCCATCCGCA CTGGCAATCC CCAGATGGAC GCATTTTACA TGATGTTCCC GGCGTTGGGC 
AGCCTGCTCA AGTGGGGCAG CCTTTTCCCA GCCTACTCGA TTCTGGGCGC CATTCCCGAC 
AATCTACAGC CTACAGCGGC AGCCTCCAAG GTGGTCCTTG TCCTGGCCGA TGATGCGACG 
GCCAAGACTC GAGTTGCCCG TCAAAATCCG CCACCAAATC CACTTGGTCA GCTAATGAAT 
TGGCCCGCTC TGCCGCAGGA CTTCCAACTG CCTTCCATGG ATCTGGGACC GCAAGTGGGC 
TCGTTTTTGG CCCAACTGCC TGCTATGCCT ACCGTGCCTG GTCTTCTGGG TGCCGCTGCT 
CCAGTTCCTG CTCCAGCTCC TGCTCCCGCT GCTGCTCCTC CTCCGGCGCC AGCTCCAGCT 
GCAGATCCTC CGGCAGCACC TGTTCCAGAT GCACCCCAAC CAGCCATACT GGGACAAGCC 
GCTCTGCAGA ACGCTTTCAC CTTCTTTAAC CCGGCTAACT TTGATGCCTC CAGTCTTCTG 
GGCCAGAGTG TACCCACATT TGCTCCTCCA AATCTTGATT TCGTCGCTCA AATGCAAAGG 
CAATTCTTCC CAGGAATGAC ACCGGCACAA CCTGCTGCCG CTGGCACGGA TGCCCAGGCC 
TCCGACATTT CCGAGGTAAG GGTACGTCCT GAGGATCCGT ATTCGCAAGA GGCACAGATG 
AAGATCAAAT CGGCACTTGA AATGGAGCAG GAGAGGCAAC AACAGGCTCA GGTCAAGGAT 
CAGGAGCAAG TGCCTCTCCT CTGGTTCCGA ATGCCCACAA CTCAGAATCA GGATGCGACT 
GAAGAAAAGA CTCTGGAGGA TCTGCGGGTC GAGGCCAAAT TGAGGGCATT CGAGCGCCAG 
GTGATAGCCG AATTGAGAAT GCTGCAGAAG ATCGAACTTA TGGCTAAGCA GATGAGGTCC 
AGCGCCGCAG CTCAAAACGG AGATTCACCC TACAGAATCA GTTATCCACT GAGTCGGACA 
CCCATTCACA AGATCACCCG TGCTGATATT GAGCAAGCCC TTCGGGATGA TTATGTCCGC 
CGATTGGTCA ACAAGGAAGC GCAACGAAGG GCTAGAAATT CCGGCATAAA CACCCAGAAG 
GCAAATGCCT TGAAGCGACA GGCCAAATCC CAGGATCAGA CTCTGTCCAA GGAGGATATC 
GTCCAGATTA TGGCGTATGC CTATCGCATG GCCAACGAAC AGATGGAGAG CGAGAAGGGC 
AAGCAGGACA AGGTTTACGC GGCCTACAGG ACGGAACAGA ATCCAATGAT GATGCAGCAA 
AGACAATGGT CGGAGGAGCA GGCCAAGATC CAACAGAATC AACAGCAGAT CCAGCAAAAT 
CCAATGATGA TGCAGCAACG ACAATGGTCG GAGGAGCAGG CCAAGATCCA ACAGAATCAA 
CAGCAGATCC AGCAAAATCC AATGATGATG CAGCAACGAC AATGGTCGGA GGAGCAGGCC 
AAGATCCAAC AGAATCAACA GCAGATCCAG CAAAATCCAA TGATGATGCA GCAAAGACAA 
TGGTCGGAGG AGCAGGCTAA GATCCAACAG AATCAACAGC AGATCCAGCA AAATCCAATG 
ATGGTGCAGC AAAGACAATG GTCGGAGGAG CAGGCCAAGA TCCAACAGAA TCAACAGCAG 
ATCCAGCAAA ATCCAATGAT GATGCAGCAA AGACAATGGT CGGAGGAGCA GGCCAAGATT 
CAGCATGATC AACAGATGGC ACAACAGATG GCACAGCAAG GTCTCATGAT GACGGAGCAG 
AGGCAAAGGC AGTGGTCAGA AGATCAGGCC AAAATTCAGC AGGCTCAACA GATGGCCCAA 
CAGACACCCA TGATGATGCC ACAGATGCAA CAAAGGCAGT GGACAGAGGA TCCCCAAATG 
GTTCAGCAGA TGCAACAGAG GCAGTGGGCA GAAGATCAGA CCAGGATGCA AATGGCCCAA 
CAGAATCCAA TGATGCAACA GCAGCGACAG ATGGCAGAGA ATCCGCAAAT GATGCAGCAA 
AGGCAATGGT CCGAGGAGCA GACCAAAATC GAACAGGCTC AGCAGATGGC ACAACAGAAT 
CAAATGATGA TGCAGCAGAT GCAGCAAAGA CAATGGTCGG AGGATCAGGC CCAGATTCAA 
CAGCAGCAGA GACAGATGAT GCAGCAGACC CCCATGATGA TGAAGGAGCG CCAGTGGGCG 
GAGGAGAACC CCCAGTCTGT CCAGCAGCAA GGGCCCATGA TGATGCAGCA GCAGATGCCA 
TCGATGATGC AGCGTGAAGT AGAGGACGAA GATAATAAGG CAGAGGATGA CTTAGTTGGC 
GAGGCGGGAC CCCAAATGCC GGAGAACGAA GGCACCGCCA GGCACAAAGT CGATGCCCTG 
GGAGTTGGCG GCAACAAGCG CAAGAAGTCC AAGTCCAAGT CGGCGCCGCC AACGGTAATT 
AACTATTACT ATGCGGCACC CCAGCGTCCG GTGGTTCAGA GTTACGGAAC AAGCTACGGC 
GGAGGTGGCT ATGGATCGAA TGCCTATGGT GTTCCTCGTC CAGTCAATTC GTATCAAAGT 
CAAGGCTACA GGGCTGCCGT GGGTAACGAT GAGGTAGATG AGATGTTGCG CCAGCACCAG 
ACAATGGCCA GGTCCATCAT CAGCACCATC CGAAACCGAA ATCGAAAACG CACCATCATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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dec-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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