pon cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pon
Entrez Gene ID	31394
Full Name	partner of numb
Synonyms	CG3346,Dmel\CG3346,PON,Pon
General protein information	Preferred Names partner of numb Names CG3346 gene product from transcript CG3346-RA CG3346-PA pon-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 4C10-4C10

mRNA and Protein(s)

mRNA	Protein	Name
NM_080333.3	NP_525072.2	partner of numb

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pon overexpression alters polar protein distribution is relevant because Pon is often used as a polarity marker in dividing progenitors.
Title: Overexpression of partner of numb induces asymmetric distribution of the PI4P 5-Kinase Skittles in mitotic sensory organ precursor cells in Drosophila.

Polo directly phosphorylates Partner of Numb (Pon), an adaptor protein for Numb, and this phosphorylation event is important for Pon to localize Numb
Title: Polo inhibits progenitor self-renewal and regulates Numb asymmetry by phosphorylating Pon.

Numb and Pon rapidly exchange between a cytoplasmic pool and the cell cortex and that preferential recruitment from the cytoplasm is responsible for their asymmetric distribution during mitosis.
Title: Quantitative analysis of protein dynamics during asymmetric cell division.

The following pon gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pon cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00790	NM_080333.3 Latest version!	Drosophila melanogaster partner of numb (pon), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pon ( NM_080333.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_525072.2 Drosophila melanogaster partner of numb (pon), mRNA.

Price & Availability↑

CloneID	OFa00790
Clone ID Related Accession (Same CDS sequence)	NM_080333.3
Accession Version	NM_080333.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	partner of numb
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_080333.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGGAGA CGAAGAGCAT AGCTGCATTG GCTTTCACGG AGACGCCGCG CAAACGCAAA 
AGGGAGACGC TCTATAAAAA TGCTGCCCCG CCCCCGAATC CGCAGCAACA ACCCCCAGAA 
AAGGACGCAC AGGATGCAGC TGTCGCGTCG ACAGAATCCT GCTTCACCAA CGCCGCCTTC 
AGTTCCACGC CCAAGAAGCT AATGGTGGCT CGTCGTCGTT TGGCCAAGGA GAATAGCCAA 
CCCAATCCGT TTCCCGGCTT GGAGGTCAAG TCCATAGCTG ATCTGGCCCA GAAGCAGCAG 
CACCAGCAGC ATCATCAGCA GCAGCAACAA CAACAACAAA AGCTACCCAC GAATCCTTTT 
GAGGTCCTGC GCCAGCCGCC CAAGAAGAAG AAGCGCGAGC ATGCCTGCTT CGAGAATCCT 
GGCCTGAATT TGGAGCTGCC CGAGAAGCAG TTCAATCCAT ATGAGGTCGT CCGCTCGGCA 
ACCACGCCCG CCAAGGGCTT CGTGAATCCT GCCCTGAATT TGCGCGGCAG TGATGCGCCG 
GCCTCGCTCA ATCCCTTCGA GATCCATCGG CCCAGCGAGG CGTCCGAGGC AGCCTGCAAT 
CCCTCGGGTG TGGCCAATCC GGCACTGGCC GATAGGGATT CGGAGGAGCA GCTGCCCACC 
AGCCTGAAGA TCGGCTTGCC ATTCACGCCA ACGCTGGGCT GTCGCATCGA TTTTCACGGC 
ATGTCCCTTA CGCAGCTAAC GCCTAGCAAG CTGCTGGCCG AGAAGCTGGT CTTTTCGCCC 
GTTCCGGCGC CCAAACGATC GCTGGGCGCC ATCAGCGAGG AGTCGTCTAT GGACATTGGC 
AAGGAGCTGG ATCGCTATCA ACTGGAATTG GAGAACAGCA TCAACGAGGC GAAGCTACGG 
AAGAATGGCG TGCTGGTGGA TAGAGAGTTG CCCAGGAATA GCTTGGAGGT GGAACTGCCC 
AAGAACACCA AGGTCTCCTT GGTCATGGAG ACCAATACGC AGGAGCTGAT GATGCAGGAA 
GTGGTGACGG TGGACACTCA AGTGGAGAGG CGTCTGGTTT GCACCAGACG ACGCACACTA 
ACCGAGATTA GCGAGGCTTC CGAGGTGGAA GAGGACACGG AGAAGCTGCT CGAGCAGGAC 
CGCGAAGCAG AGGTGATTCT CGAGCAGGAG AAGATTCTAG AGCAGGAGAT GGTCTCAGAG 
CGGGAACGAC ATTTAACGAG GGAGAAGCAA TTGAAGCAGG AGAAACTCCT AGAGAGGGAA 
AAACATTTGG AGCGAGAGAA GCTGCAGGAG AAGCTGCACG AGCAGCTCAG GGAAAAGCTC 
CAGGAGAGGG CCAAGCATCT GGAGAAGGAG AAGCTGGAGG AGGAGGAGCT CCTGGAAAGG 
CAGCTAGAAG AGGAACGGGA AAAGGAGCCA ACCGACGGCG ATGTGGCCTA TGCCTCCGAA 
TCCGAATCCG ACGACGAGCC CGATGATCTA GACTTCAAGG CACCCGCGCG CTTCGTTCGC 
GCCTACAGAC CCGCCGCTCT GCCCTCCAAG GCGGCCAGCA AGGAGTCGCT GCAGTCCATC 
GGATCAAGCA AGTCCGCCAA ATCGGCAGAA GTTAAGCCCA GTATCGGAAT GAAAGGCATG 
ATAAGAAAGT CCATTCGAAG ACTGATGCAT CCCACGTCGC ACACCACGCC ATCCGAGGTA 
AAGTCCGAGG ACAAGGATGA ACATGGACAT GGACAGGGCC ATGGACAGCA CAACATACTC 
AATAGCATTC GGCACAGCCT GCGCAGGAGG CCACAAAAGG CGGCCGAGTT GGAGGAGCAA 
ATGGAGCCCG TTCTCGCCGA CGTCTCCATT ATCGACACCA GCGAACGGAC CATGAAGCTC 
CGCTCGAGCG TTGCCCAAAC CGAGTACATG ACCATCGAGC AGCTGACCAA CGAGAAGAAG 
CACTCACTGC GGAACAGCAT TAGGCGGTCC ACGCGGGACG TGCTGCGCCA TGTGTTCCAC 
AAGAGTCACG ATGCCTATGC AACCGCCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_525072.2
CDS	136..2148
Translation	MLETKSIAALAFTETPRKRKRETLYKNAAPPPNPQQQPPEKDAQDAAVASTESCFTNAAFSSTPKKLMVARRRLAKENSQPNPFPGLEVKSIADLAQKQQHQQHHQQQQQQQQKLPTNPFEVLRQPPKKKKREHACFENPGLNLELPEKQFNPYEVVRSATTPAKGFVNPALNLRGSDAPASLNPFEIHRPSEASEAACNPSGVANPALADRDSEEQLPTSLKIGLPFTPTLGCRIDFHGMSLTQLTPSKLLAEKLVFSPVPAPKRSLGAISEESSMDIGKELDRYQLELENSINEAKLRKNGVLVDRELPRNSLEVELPKNTKVSLVMETNTQELMMQEVVTVDTQVERRLVCTRRRTLTEISEASEVEEDTEKLLEQDREAEVILEQEKILEQEMVSERERHLTREKQLKQEKLLEREKHLEREKLQEKLHEQLREKLQERAKHLEKEKLEEEELLERQLEEEREKEPTDGDVAYASESESDDEPDDLDFKAPARFVRAYRPAALPSKAASKESLQSIGSSKSAKSAEVKPSIGMKGMIRKSIRRLMHPTSHTTPSEVKSEDKDEHGHGQGHGQHNILNSIRHSLRRRPQKAAELEEQMEPVLADVSIIDTSERTMKLRSSVAQTEYMTIEQLTNEKKHSLRNSIRRSTRDVLRHVFHKSHDAYATAK

Target ORF information:

RefSeq Version	NM_080333.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster partner of numb (pon), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080333.3

ORF Insert Sequence:

ATGCTGGAGA CGAAGAGCAT AGCTGCATTG GCTTTCACGG AGACGCCGCG CAAACGCAAA 
AGGGAGACGC TCTATAAAAA TGCTGCCCCG CCCCCGAATC CGCAGCAACA ACCCCCAGAA 
AAGGACGCAC AGGATGCAGC TGTCGCGTCG ACAGAATCCT GCTTCACCAA CGCCGCCTTC 
AGTTCCACGC CCAAGAAGCT AATGGTGGCT CGTCGTCGTT TGGCCAAGGA GAATAGCCAA 
CCCAATCCGT TTCCCGGCTT GGAGGTCAAG TCCATAGCTG ATCTGGCCCA GAAGCAGCAG 
CACCAGCAGC ATCATCAGCA GCAGCAACAA CAACAACAAA AGCTACCCAC GAATCCTTTT 
GAGGTCCTGC GCCAGCCGCC CAAGAAGAAG AAGCGCGAGC ATGCCTGCTT CGAGAATCCT 
GGCCTGAATT TGGAGCTGCC CGAGAAGCAG TTCAATCCAT ATGAGGTCGT CCGCTCGGCA 
ACCACGCCCG CCAAGGGCTT CGTGAATCCT GCCCTGAATT TGCGCGGCAG TGATGCGCCG 
GCCTCGCTCA ATCCCTTCGA GATCCATCGG CCCAGCGAGG CGTCCGAGGC AGCCTGCAAT 
CCCTCGGGTG TGGCCAATCC GGCACTGGCC GATAGGGATT CGGAGGAGCA GCTGCCCACC 
AGCCTGAAGA TCGGCTTGCC ATTCACGCCA ACGCTGGGCT GTCGCATCGA TTTTCACGGC 
ATGTCCCTTA CGCAGCTAAC GCCTAGCAAG CTGCTGGCCG AGAAGCTGGT CTTTTCGCCC 
GTTCCGGCGC CCAAACGATC GCTGGGCGCC ATCAGCGAGG AGTCGTCTAT GGACATTGGC 
AAGGAGCTGG ATCGCTATCA ACTGGAATTG GAGAACAGCA TCAACGAGGC GAAGCTACGG 
AAGAATGGCG TGCTGGTGGA TAGAGAGTTG CCCAGGAATA GCTTGGAGGT GGAACTGCCC 
AAGAACACCA AGGTCTCCTT GGTCATGGAG ACCAATACGC AGGAGCTGAT GATGCAGGAA 
GTGGTGACGG TGGACACTCA AGTGGAGAGG CGTCTGGTTT GCACCAGACG ACGCACACTA 
ACCGAGATTA GCGAGGCTTC CGAGGTGGAA GAGGACACGG AGAAGCTGCT CGAGCAGGAC 
CGCGAAGCAG AGGTGATTCT CGAGCAGGAG AAGATTCTAG AGCAGGAGAT GGTCTCAGAG 
CGGGAACGAC ATTTAACGAG GGAGAAGCAA TTGAAGCAGG AGAAACTCCT AGAGAGGGAA 
AAACATTTGG AGCGAGAGAA GCTGCAGGAG AAGCTGCACG AGCAGCTCAG GGAAAAGCTC 
CAGGAGAGGG CCAAGCATCT GGAGAAGGAG AAGCTGGAGG AGGAGGAGCT CCTGGAAAGG 
CAGCTAGAAG AGGAACGGGA AAAGGAGCCA ACCGACGGCG ATGTGGCCTA TGCCTCCGAA 
TCCGAATCCG ACGACGAGCC CGATGATCTA GACTTCAAGG CACCCGCGCG CTTCGTTCGC 
GCCTACAGAC CCGCCGCTCT GCCCTCCAAG GCGGCCAGCA AGGAGTCGCT GCAGTCCATC 
GGATCAAGCA AGTCCGCCAA ATCGGCAGAA GTTAAGCCCA GTATCGGAAT GAAAGGCATG 
ATAAGAAAGT CCATTCGAAG ACTGATGCAT CCCACGTCGC ACACCACGCC ATCCGAGGTA 
AAGTCCGAGG ACAAGGATGA ACATGGACAT GGACAGGGCC ATGGACAGCA CAACATACTC 
AATAGCATTC GGCACAGCCT GCGCAGGAGG CCACAAAAGG CGGCCGAGTT GGAGGAGCAA 
ATGGAGCCCG TTCTCGCCGA CGTCTCCATT ATCGACACCA GCGAACGGAC CATGAAGCTC 
CGCTCGAGCG TTGCCCAAAC CGAGTACATG ACCATCGAGC AGCTGACCAA CGAGAAGAAG 
CACTCACTGC GGAACAGCAT TAGGCGGTCC ACGCGGGACG TGCTGCGCCA TGTGTTCCAC 
AAGAGTCACG ATGCCTATGC AACCGCCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pon cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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