Ocrl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ocrl
Entrez Gene ID	31157
Full Name	Oculocerebrorenal syndrome of Lowe
Synonyms	CG3573,Dmel\CG3573,EG:86E4.5,dOCRL,ocrl
General protein information	Preferred Names Oculocerebrorenal syndrome of Lowe Names CG3573 gene product from transcript CG3573-RA CG3573-PA CG3573-PB Ocrl-PA Ocrl-PB inositol 5-phosphatase inositol polyphosphate 5-phosphatase oculocerebrorenal syndrome of lowe oculocerebrorenal syndrome of lowe 1 ortholog oculocerebrorenal syndrome of lowe ortholog
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 2B16-2B16

mRNA and Protein(s)

mRNA	Protein	Name
NM_130606.3	NP_569962.2	oculocerebrorenal syndrome of lowe, isoform A
NM_001272224.1	NP_001259153.1	oculocerebrorenal syndrome of lowe, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	OCRL	NP_000267.2
Pan troglodytes (chimpanzee)	OCRL	XP_003317719.2
Rattus norvegicus (Norway rat)	Ocrl	NP_001101726.2
Canis lupus familiaris (dog)	OCRL	XP_852376.2
Mus musculus (house mouse)	Ocrl	NP_796189.2
Xenopus tropicalis (tropical clawed frog)	ocrl	XP_004919252.1
Gallus gallus (chicken)	OCRL	XP_004940632.1
Danio rerio (zebrafish)	LOC100329312	XP_003199958.1
	OCRL	XP_002806438.1
Danio rerio (zebrafish)	ocrl	NP_001071232.1
Drosophila melanogaster (fruit fly)	Ocrl	NP_569962.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010556	XP_558090.3
Bos taurus (cattle)	OCRL	NP_001095661.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

docrl ( phosphatidylinositol-5-phosphatase OCRL)regulation of endosomal traffic maintains hemocytes in a poised, but quiescent state, suggesting mechanisms by which endosomal misregulation of signaling may contribute to symptoms of Lowe syndrome
Title: dOCRL maintains immune cell quiescence by regulating endosomal traffic.

The following Ocrl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ocrl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00534	NM_130606.3 Latest version!	Drosophila melanogaster oculocerebrorenal syndrome of lowe, transcript variant A (Ocrl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa00534	NM_001272224.1 Latest version!	Drosophila melanogaster oculocerebrorenal syndrome of lowe, transcript variant B (Ocrl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ocrl ( NM_130606.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_569962.2 Drosophila melanogaster oculocerebrorenal syndrome of lowe, transcript variant A (Ocrl), mRNA.

Price & Availability↑

CloneID	OFa00534
Clone ID Related Accession (Same CDS sequence)	NM_130606.3 , NM_001272224.1
Accession Version	NM_130606.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	oculocerebrorenal syndrome of lowe, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_130606.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACACGT TGAGCGAGGC AGTGGCCAAT GGAACCGCGA CTGCGGCCAC CAGGACGACC 
AAGGACATTG TCAAGGAGCG CTTCAAGGAG GACGAGACCA TAGAATACAT ATTCGAGGCG 
TATCAGATCA AAGGACCAGA GTACTCCAAC CGTCTTCTGG CCCTGGTTTC ATCGCAGTCG 
GGCGGCACGT TTGCCATCAT CGCGTTCTCC TACTTGCGCA CTCCATTGTC CTCCGCGAAC 
GAGCTGATCA TCAACAAGGT GTTCGCAATC GACCACAATT TCCAGTTGCG ACAGGACAGC 
AAGAGCTCGA TCACCACGCA ACAGTTTGAC CTGTCCACAG CCGAGGATGG CCCCATCAAG 
TACTACTACT ACGCCACGGA GAGCCACCAC TACGAGGAGT TTGTGGCCAA GGTGATTTCC 
TTCAAGAGCA CCATGGCGCA GCATGATCCC GAGACGGTGC TTAACTTCAG GTGGCTGAAT 
GACTACCGGC AAATCGGAGA AGTGAAGCAG GAGCTGAAGA AGCGGGAGAG CGAGTACATT 
GTCTACAAGG ACATAATCAT CTACTGCGCC ACTTGGAATG TGAACAACAA GACCTGCAGC 
GACAGCAACA ATCCACTGCG AGCCTGGCTG GCTTGCAGTG AGAAGCCGCC GGATATCTAC 
GCGATTGGAC TGCAGGAGCT AGATACTCCC ACAAAGGCTA TGTTGAACTC CACCCAGGTG 
CAGGCCATCG AGAAGCAATG GATCGATAAG ATGATGGACA GCGTCCACCC TGACGTAGAG 
TACGAAATCC TGATGTCGCA CCGCCTGGTG GCCACCATGC TGACGGTCAT CGTGCGCAAG 
CAGCTGCGGC AGCACATCAT ACGCTGCCGA CCAAAGTCGG TGGCCCGCGG CATCTTCAAT 
ACGCTAGGCA ACAAGGGCGG TGTCGCCATT AGCCTGCAGC TCAACGAGGG CAACATCTGC 
TTTGTGAACT CCCACCTGGC CGCCCACATG GGCTACGTGG AGGAGCGGAA TCAGGACTAC 
AACGCTATCG TGGAGGGCAT ACGCTTCGAT GATGGCCGAA CAATCAGTGA CCATGATCAC 
ATCTTCTGGG TCGGCGATTT GAATTACCGC ATCCAGGAGC CGCCCGGCCA ACAGCGACCA 
GGACCACTGA GCGATGCCCA GACATACGAG CTGCTACTGC AGTACGACCA GCTACGTCAG 
GAGATGCGGC GTGGAAAGTG CTTTGAGGGC TACACCGAGG GCGAGATCAA GTTCCGGCCG 
ACGTACAAGT ATGATCCGGG CACGGACAAC TACGACAGCA GCGAGAAGCA GCGGGCGCCG 
GCCTACTGCG ATCGAGTGCT CTGGAAGGGC ACGCGCATCG AGCAGCTGGC CTACAACAGC 
ATCATGGAGA TTCGGCAGAG CGATCACAAG CCCGTGTACG CCGTGTTCCA GGTCAAGGTC 
AAGACTCGCG ATGAGGTCAA GTACAAACGG GTGCAGGAGG AGGTGCTGAA GGCAGTGGAC 
AAGCGGGAGA ACGACAACCA GCCACAGATC AATGTCGAGA AGACGGTCAT CGATTTCGGG 
ACTGTGCGAT TTAACGAGCC GAGCACACGG GACTTCAACG TCTATAACAA CTGCCCACTA 
CCTGTGGACT TCAGTTTCAA AGAGAAGGAT ATACACGCCA TCTGCGAGCC ATGGCTGCAC 
GTCGATCCGC GCCAAGACTC GCTGCTGATC GACTCCGCCC GAAGCATTCG GCTGAAAATG 
AACGCCAATG TGCGCACCAT TGCCGGCTTG CTTCGCAAGA TCCGTGCTAG CGACAACTTC 
GATATTCTCA TCCTGCACGT GGAGAACGGG CGTGACATCT TCATCACGGT TACGGGCGAC 
TATCAGCCCA GTTGCTTTGG CCTTTCCATG GAGACGATGT GCCGCACCGA CCGTCCATTA 
AGCGAGTATT CCCAAGATCA GATTAAGCAA CTCATGAATG ACGAATCACC GGAATACCGT 
GTGACGATGC CCCGGGAATT CTTTCTGCTG ATCGACTATC TGTACAGGCA GGGCTCCAAG 
CAGGTGGGCG CCTTCCCGTC CTACGACTCG CGTCTCTCCC TCGGCGCTCA ATTTAACTCG 
GTGCGCGATT GGTTGGACAC CTGGTCGGAT GATCCGTTCC CTGCCAACGC AGAGACGGCT 
GCGCAGGCGC TGCTGCTTTT GCTGGATCTG CCGGAGCATG CTCTGCTGGA GCCGGTGGTC 
GAGAATCTGC TGGAGTGCAC CAACAAGTCG CAGGCTATGG ACTACATTTC GCTGTTAAGT 
CCCCCCAAGC GCAATGTTTT CATGCACCTT TGTATGTTCC TGCGCGCGGG CATCGAGAGT 
CAGTTCTATG ATCTGCATCA AGTCGCATCC ACCTTTGGCC GCATTCTGTT GCGAAGCACC 
GAGCGTGCCG CCTGGATGGA CTACCACAGC CGATGCATAC AGTTCATGCG GCTCTTCATC 
GACACCGACG TCGAGGCAAT GGGCAACGGC AATGAGGGCG CCGGAACTGG AACAGGCTCG 
GGATCTGGGA CCCGGGCGGG TCTGCAGGCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569962.2
CDS	778..3330
Translation	MDTLSEAVANGTATAATRTTKDIVKERFKEDETIEYIFEAYQIKGPEYSNRLLALVSSQSGGTFAIIAFSYLRTPLSSANELIINKVFAIDHNFQLRQDSKSSITTQQFDLSTAEDGPIKYYYYATESHHYEEFVAKVISFKSTMAQHDPETVLNFRWLNDYRQIGEVKQELKKRESEYIVYKDIIIYCATWNVNNKTCSDSNNPLRAWLACSEKPPDIYAIGLQELDTPTKAMLNSTQVQAIEKQWIDKMMDSVHPDVEYEILMSHRLVATMLTVIVRKQLRQHIIRCRPKSVARGIFNTLGNKGGVAISLQLNEGNICFVNSHLAAHMGYVEERNQDYNAIVEGIRFDDGRTISDHDHIFWVGDLNYRIQEPPGQQRPGPLSDAQTYELLLQYDQLRQEMRRGKCFEGYTEGEIKFRPTYKYDPGTDNYDSSEKQRAPAYCDRVLWKGTRIEQLAYNSIMEIRQSDHKPVYAVFQVKVKTRDEVKYKRVQEEVLKAVDKRENDNQPQINVEKTVIDFGTVRFNEPSTRDFNVYNNCPLPVDFSFKEKDIHAICEPWLHVDPRQDSLLIDSARSIRLKMNANVRTIAGLLRKIRASDNFDILILHVENGRDIFITVTGDYQPSCFGLSMETMCRTDRPLSEYSQDQIKQLMNDESPEYRVTMPREFFLLIDYLYRQGSKQVGAFPSYDSRLSLGAQFNSVRDWLDTWSDDPFPANAETAAQALLLLLDLPEHALLEPVVENLLECTNKSQAMDYISLLSPPKRNVFMHLCMFLRAGIESQFYDLHQVASTFGRILLRSTERAAWMDYHSRCIQFMRLFIDTDVEAMGNGNEGAGTGTGSGSGTRAGLQA

Target ORF information:

RefSeq Version	NM_130606.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster oculocerebrorenal syndrome of lowe, transcript variant A (Ocrl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130606.3

ORF Insert Sequence:

ATGGACACGT TGAGCGAGGC AGTGGCCAAT GGAACCGCGA CTGCGGCCAC CAGGACGACC 
AAGGACATTG TCAAGGAGCG CTTCAAGGAG GACGAGACCA TAGAATACAT ATTCGAGGCG 
TATCAGATCA AAGGACCAGA GTACTCCAAC CGTCTTCTGG CCCTGGTTTC ATCGCAGTCG 
GGCGGCACGT TTGCCATCAT CGCGTTCTCC TACTTGCGCA CTCCATTGTC CTCCGCGAAC 
GAGCTGATCA TCAACAAGGT GTTCGCAATC GACCACAATT TCCAGTTGCG ACAGGACAGC 
AAGAGCTCGA TCACCACGCA ACAGTTTGAC CTGTCCACAG CCGAGGATGG CCCCATCAAG 
TACTACTACT ACGCCACGGA GAGCCACCAC TACGAGGAGT TTGTGGCCAA GGTGATTTCC 
TTCAAGAGCA CCATGGCGCA GCATGATCCC GAGACGGTGC TTAACTTCAG GTGGCTGAAT 
GACTACCGGC AAATCGGAGA AGTGAAGCAG GAGCTGAAGA AGCGGGAGAG CGAGTACATT 
GTCTACAAGG ACATAATCAT CTACTGCGCC ACTTGGAATG TGAACAACAA GACCTGCAGC 
GACAGCAACA ATCCACTGCG AGCCTGGCTG GCTTGCAGTG AGAAGCCGCC GGATATCTAC 
GCGATTGGAC TGCAGGAGCT AGATACTCCC ACAAAGGCTA TGTTGAACTC CACCCAGGTG 
CAGGCCATCG AGAAGCAATG GATCGATAAG ATGATGGACA GCGTCCACCC TGACGTAGAG 
TACGAAATCC TGATGTCGCA CCGCCTGGTG GCCACCATGC TGACGGTCAT CGTGCGCAAG 
CAGCTGCGGC AGCACATCAT ACGCTGCCGA CCAAAGTCGG TGGCCCGCGG CATCTTCAAT 
ACGCTAGGCA ACAAGGGCGG TGTCGCCATT AGCCTGCAGC TCAACGAGGG CAACATCTGC 
TTTGTGAACT CCCACCTGGC CGCCCACATG GGCTACGTGG AGGAGCGGAA TCAGGACTAC 
AACGCTATCG TGGAGGGCAT ACGCTTCGAT GATGGCCGAA CAATCAGTGA CCATGATCAC 
ATCTTCTGGG TCGGCGATTT GAATTACCGC ATCCAGGAGC CGCCCGGCCA ACAGCGACCA 
GGACCACTGA GCGATGCCCA GACATACGAG CTGCTACTGC AGTACGACCA GCTACGTCAG 
GAGATGCGGC GTGGAAAGTG CTTTGAGGGC TACACCGAGG GCGAGATCAA GTTCCGGCCG 
ACGTACAAGT ATGATCCGGG CACGGACAAC TACGACAGCA GCGAGAAGCA GCGGGCGCCG 
GCCTACTGCG ATCGAGTGCT CTGGAAGGGC ACGCGCATCG AGCAGCTGGC CTACAACAGC 
ATCATGGAGA TTCGGCAGAG CGATCACAAG CCCGTGTACG CCGTGTTCCA GGTCAAGGTC 
AAGACTCGCG ATGAGGTCAA GTACAAACGG GTGCAGGAGG AGGTGCTGAA GGCAGTGGAC 
AAGCGGGAGA ACGACAACCA GCCACAGATC AATGTCGAGA AGACGGTCAT CGATTTCGGG 
ACTGTGCGAT TTAACGAGCC GAGCACACGG GACTTCAACG TCTATAACAA CTGCCCACTA 
CCTGTGGACT TCAGTTTCAA AGAGAAGGAT ATACACGCCA TCTGCGAGCC ATGGCTGCAC 
GTCGATCCGC GCCAAGACTC GCTGCTGATC GACTCCGCCC GAAGCATTCG GCTGAAAATG 
AACGCCAATG TGCGCACCAT TGCCGGCTTG CTTCGCAAGA TCCGTGCTAG CGACAACTTC 
GATATTCTCA TCCTGCACGT GGAGAACGGG CGTGACATCT TCATCACGGT TACGGGCGAC 
TATCAGCCCA GTTGCTTTGG CCTTTCCATG GAGACGATGT GCCGCACCGA CCGTCCATTA 
AGCGAGTATT CCCAAGATCA GATTAAGCAA CTCATGAATG ACGAATCACC GGAATACCGT 
GTGACGATGC CCCGGGAATT CTTTCTGCTG ATCGACTATC TGTACAGGCA GGGCTCCAAG 
CAGGTGGGCG CCTTCCCGTC CTACGACTCG CGTCTCTCCC TCGGCGCTCA ATTTAACTCG 
GTGCGCGATT GGTTGGACAC CTGGTCGGAT GATCCGTTCC CTGCCAACGC AGAGACGGCT 
GCGCAGGCGC TGCTGCTTTT GCTGGATCTG CCGGAGCATG CTCTGCTGGA GCCGGTGGTC 
GAGAATCTGC TGGAGTGCAC CAACAAGTCG CAGGCTATGG ACTACATTTC GCTGTTAAGT 
CCCCCCAAGC GCAATGTTTT CATGCACCTT TGTATGTTCC TGCGCGCGGG CATCGAGAGT 
CAGTTCTATG ATCTGCATCA AGTCGCATCC ACCTTTGGCC GCATTCTGTT GCGAAGCACC 
GAGCGTGCCG CCTGGATGGA CTACCACAGC CGATGCATAC AGTTCATGCG GCTCTTCATC 
GACACCGACG TCGAGGCAAT GGGCAACGGC AATGAGGGCG CCGGAACTGG AACAGGCTCG 
GGATCTGGGA CCCGGGCGGG TCTGCAGGCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ocrl ( NM_001272224.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259153.1 Drosophila melanogaster oculocerebrorenal syndrome of lowe, transcript variant B (Ocrl), mRNA.

Price & Availability↑

CloneID	OFa00534
Clone ID Related Accession (Same CDS sequence)	NM_130606.3 , NM_001272224.1
Accession Version	NM_001272224.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	oculocerebrorenal syndrome of lowe, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACACGT TGAGCGAGGC AGTGGCCAAT GGAACCGCGA CTGCGGCCAC CAGGACGACC 
AAGGACATTG TCAAGGAGCG CTTCAAGGAG GACGAGACCA TAGAATACAT ATTCGAGGCG 
TATCAGATCA AAGGACCAGA GTACTCCAAC CGTCTTCTGG CCCTGGTTTC ATCGCAGTCG 
GGCGGCACGT TTGCCATCAT CGCGTTCTCC TACTTGCGCA CTCCATTGTC CTCCGCGAAC 
GAGCTGATCA TCAACAAGGT GTTCGCAATC GACCACAATT TCCAGTTGCG ACAGGACAGC 
AAGAGCTCGA TCACCACGCA ACAGTTTGAC CTGTCCACAG CCGAGGATGG CCCCATCAAG 
TACTACTACT ACGCCACGGA GAGCCACCAC TACGAGGAGT TTGTGGCCAA GGTGATTTCC 
TTCAAGAGCA CCATGGCGCA GCATGATCCC GAGACGGTGC TTAACTTCAG GTGGCTGAAT 
GACTACCGGC AAATCGGAGA AGTGAAGCAG GAGCTGAAGA AGCGGGAGAG CGAGTACATT 
GTCTACAAGG ACATAATCAT CTACTGCGCC ACTTGGAATG TGAACAACAA GACCTGCAGC 
GACAGCAACA ATCCACTGCG AGCCTGGCTG GCTTGCAGTG AGAAGCCGCC GGATATCTAC 
GCGATTGGAC TGCAGGAGCT AGATACTCCC ACAAAGGCTA TGTTGAACTC CACCCAGGTG 
CAGGCCATCG AGAAGCAATG GATCGATAAG ATGATGGACA GCGTCCACCC TGACGTAGAG 
TACGAAATCC TGATGTCGCA CCGCCTGGTG GCCACCATGC TGACGGTCAT CGTGCGCAAG 
CAGCTGCGGC AGCACATCAT ACGCTGCCGA CCAAAGTCGG TGGCCCGCGG CATCTTCAAT 
ACGCTAGGCA ACAAGGGCGG TGTCGCCATT AGCCTGCAGC TCAACGAGGG CAACATCTGC 
TTTGTGAACT CCCACCTGGC CGCCCACATG GGCTACGTGG AGGAGCGGAA TCAGGACTAC 
AACGCTATCG TGGAGGGCAT ACGCTTCGAT GATGGCCGAA CAATCAGTGA CCATGATCAC 
ATCTTCTGGG TCGGCGATTT GAATTACCGC ATCCAGGAGC CGCCCGGCCA ACAGCGACCA 
GGACCACTGA GCGATGCCCA GACATACGAG CTGCTACTGC AGTACGACCA GCTACGTCAG 
GAGATGCGGC GTGGAAAGTG CTTTGAGGGC TACACCGAGG GCGAGATCAA GTTCCGGCCG 
ACGTACAAGT ATGATCCGGG CACGGACAAC TACGACAGCA GCGAGAAGCA GCGGGCGCCG 
GCCTACTGCG ATCGAGTGCT CTGGAAGGGC ACGCGCATCG AGCAGCTGGC CTACAACAGC 
ATCATGGAGA TTCGGCAGAG CGATCACAAG CCCGTGTACG CCGTGTTCCA GGTCAAGGTC 
AAGACTCGCG ATGAGGTCAA GTACAAACGG GTGCAGGAGG AGGTGCTGAA GGCAGTGGAC 
AAGCGGGAGA ACGACAACCA GCCACAGATC AATGTCGAGA AGACGGTCAT CGATTTCGGG 
ACTGTGCGAT TTAACGAGCC GAGCACACGG GACTTCAACG TCTATAACAA CTGCCCACTA 
CCTGTGGACT TCAGTTTCAA AGAGAAGGAT ATACACGCCA TCTGCGAGCC ATGGCTGCAC 
GTCGATCCGC GCCAAGACTC GCTGCTGATC GACTCCGCCC GAAGCATTCG GCTGAAAATG 
AACGCCAATG TGCGCACCAT TGCCGGCTTG CTTCGCAAGA TCCGTGCTAG CGACAACTTC 
GATATTCTCA TCCTGCACGT GGAGAACGGG CGTGACATCT TCATCACGGT TACGGGCGAC 
TATCAGCCCA GTTGCTTTGG CCTTTCCATG GAGACGATGT GCCGCACCGA CCGTCCATTA 
AGCGAGTATT CCCAAGATCA GATTAAGCAA CTCATGAATG ACGAATCACC GGAATACCGT 
GTGACGATGC CCCGGGAATT CTTTCTGCTG ATCGACTATC TGTACAGGCA GGGCTCCAAG 
CAGGTGGGCG CCTTCCCGTC CTACGACTCG CGTCTCTCCC TCGGCGCTCA ATTTAACTCG 
GTGCGCGATT GGTTGGACAC CTGGTCGGAT GATCCGTTCC CTGCCAACGC AGAGACGGCT 
GCGCAGGCGC TGCTGCTTTT GCTGGATCTG CCGGAGCATG CTCTGCTGGA GCCGGTGGTC 
GAGAATCTGC TGGAGTGCAC CAACAAGTCG CAGGCTATGG ACTACATTTC GCTGTTAAGT 
CCCCCCAAGC GCAATGTTTT CATGCACCTT TGTATGTTCC TGCGCGCGGG CATCGAGAGT 
CAGTTCTATG ATCTGCATCA AGTCGCATCC ACCTTTGGCC GCATTCTGTT GCGAAGCACC 
GAGCGTGCCG CCTGGATGGA CTACCACAGC CGATGCATAC AGTTCATGCG GCTCTTCATC 
GACACCGACG TCGAGGCAAT GGGCAACGGC AATGAGGGCG CCGGAACTGG AACAGGCTCG 
GGATCTGGGA CCCGGGCGGG TCTGCAGGCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259153.1
CDS	709..3261
Translation	MDTLSEAVANGTATAATRTTKDIVKERFKEDETIEYIFEAYQIKGPEYSNRLLALVSSQSGGTFAIIAFSYLRTPLSSANELIINKVFAIDHNFQLRQDSKSSITTQQFDLSTAEDGPIKYYYYATESHHYEEFVAKVISFKSTMAQHDPETVLNFRWLNDYRQIGEVKQELKKRESEYIVYKDIIIYCATWNVNNKTCSDSNNPLRAWLACSEKPPDIYAIGLQELDTPTKAMLNSTQVQAIEKQWIDKMMDSVHPDVEYEILMSHRLVATMLTVIVRKQLRQHIIRCRPKSVARGIFNTLGNKGGVAISLQLNEGNICFVNSHLAAHMGYVEERNQDYNAIVEGIRFDDGRTISDHDHIFWVGDLNYRIQEPPGQQRPGPLSDAQTYELLLQYDQLRQEMRRGKCFEGYTEGEIKFRPTYKYDPGTDNYDSSEKQRAPAYCDRVLWKGTRIEQLAYNSIMEIRQSDHKPVYAVFQVKVKTRDEVKYKRVQEEVLKAVDKRENDNQPQINVEKTVIDFGTVRFNEPSTRDFNVYNNCPLPVDFSFKEKDIHAICEPWLHVDPRQDSLLIDSARSIRLKMNANVRTIAGLLRKIRASDNFDILILHVENGRDIFITVTGDYQPSCFGLSMETMCRTDRPLSEYSQDQIKQLMNDESPEYRVTMPREFFLLIDYLYRQGSKQVGAFPSYDSRLSLGAQFNSVRDWLDTWSDDPFPANAETAAQALLLLLDLPEHALLEPVVENLLECTNKSQAMDYISLLSPPKRNVFMHLCMFLRAGIESQFYDLHQVASTFGRILLRSTERAAWMDYHSRCIQFMRLFIDTDVEAMGNGNEGAGTGTGSGSGTRAGLQA

Target ORF information:

RefSeq Version	NM_001272224.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster oculocerebrorenal syndrome of lowe, transcript variant B (Ocrl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272224.1

ORF Insert Sequence:

ATGGACACGT TGAGCGAGGC AGTGGCCAAT GGAACCGCGA CTGCGGCCAC CAGGACGACC 
AAGGACATTG TCAAGGAGCG CTTCAAGGAG GACGAGACCA TAGAATACAT ATTCGAGGCG 
TATCAGATCA AAGGACCAGA GTACTCCAAC CGTCTTCTGG CCCTGGTTTC ATCGCAGTCG 
GGCGGCACGT TTGCCATCAT CGCGTTCTCC TACTTGCGCA CTCCATTGTC CTCCGCGAAC 
GAGCTGATCA TCAACAAGGT GTTCGCAATC GACCACAATT TCCAGTTGCG ACAGGACAGC 
AAGAGCTCGA TCACCACGCA ACAGTTTGAC CTGTCCACAG CCGAGGATGG CCCCATCAAG 
TACTACTACT ACGCCACGGA GAGCCACCAC TACGAGGAGT TTGTGGCCAA GGTGATTTCC 
TTCAAGAGCA CCATGGCGCA GCATGATCCC GAGACGGTGC TTAACTTCAG GTGGCTGAAT 
GACTACCGGC AAATCGGAGA AGTGAAGCAG GAGCTGAAGA AGCGGGAGAG CGAGTACATT 
GTCTACAAGG ACATAATCAT CTACTGCGCC ACTTGGAATG TGAACAACAA GACCTGCAGC 
GACAGCAACA ATCCACTGCG AGCCTGGCTG GCTTGCAGTG AGAAGCCGCC GGATATCTAC 
GCGATTGGAC TGCAGGAGCT AGATACTCCC ACAAAGGCTA TGTTGAACTC CACCCAGGTG 
CAGGCCATCG AGAAGCAATG GATCGATAAG ATGATGGACA GCGTCCACCC TGACGTAGAG 
TACGAAATCC TGATGTCGCA CCGCCTGGTG GCCACCATGC TGACGGTCAT CGTGCGCAAG 
CAGCTGCGGC AGCACATCAT ACGCTGCCGA CCAAAGTCGG TGGCCCGCGG CATCTTCAAT 
ACGCTAGGCA ACAAGGGCGG TGTCGCCATT AGCCTGCAGC TCAACGAGGG CAACATCTGC 
TTTGTGAACT CCCACCTGGC CGCCCACATG GGCTACGTGG AGGAGCGGAA TCAGGACTAC 
AACGCTATCG TGGAGGGCAT ACGCTTCGAT GATGGCCGAA CAATCAGTGA CCATGATCAC 
ATCTTCTGGG TCGGCGATTT GAATTACCGC ATCCAGGAGC CGCCCGGCCA ACAGCGACCA 
GGACCACTGA GCGATGCCCA GACATACGAG CTGCTACTGC AGTACGACCA GCTACGTCAG 
GAGATGCGGC GTGGAAAGTG CTTTGAGGGC TACACCGAGG GCGAGATCAA GTTCCGGCCG 
ACGTACAAGT ATGATCCGGG CACGGACAAC TACGACAGCA GCGAGAAGCA GCGGGCGCCG 
GCCTACTGCG ATCGAGTGCT CTGGAAGGGC ACGCGCATCG AGCAGCTGGC CTACAACAGC 
ATCATGGAGA TTCGGCAGAG CGATCACAAG CCCGTGTACG CCGTGTTCCA GGTCAAGGTC 
AAGACTCGCG ATGAGGTCAA GTACAAACGG GTGCAGGAGG AGGTGCTGAA GGCAGTGGAC 
AAGCGGGAGA ACGACAACCA GCCACAGATC AATGTCGAGA AGACGGTCAT CGATTTCGGG 
ACTGTGCGAT TTAACGAGCC GAGCACACGG GACTTCAACG TCTATAACAA CTGCCCACTA 
CCTGTGGACT TCAGTTTCAA AGAGAAGGAT ATACACGCCA TCTGCGAGCC ATGGCTGCAC 
GTCGATCCGC GCCAAGACTC GCTGCTGATC GACTCCGCCC GAAGCATTCG GCTGAAAATG 
AACGCCAATG TGCGCACCAT TGCCGGCTTG CTTCGCAAGA TCCGTGCTAG CGACAACTTC 
GATATTCTCA TCCTGCACGT GGAGAACGGG CGTGACATCT TCATCACGGT TACGGGCGAC 
TATCAGCCCA GTTGCTTTGG CCTTTCCATG GAGACGATGT GCCGCACCGA CCGTCCATTA 
AGCGAGTATT CCCAAGATCA GATTAAGCAA CTCATGAATG ACGAATCACC GGAATACCGT 
GTGACGATGC CCCGGGAATT CTTTCTGCTG ATCGACTATC TGTACAGGCA GGGCTCCAAG 
CAGGTGGGCG CCTTCCCGTC CTACGACTCG CGTCTCTCCC TCGGCGCTCA ATTTAACTCG 
GTGCGCGATT GGTTGGACAC CTGGTCGGAT GATCCGTTCC CTGCCAACGC AGAGACGGCT 
GCGCAGGCGC TGCTGCTTTT GCTGGATCTG CCGGAGCATG CTCTGCTGGA GCCGGTGGTC 
GAGAATCTGC TGGAGTGCAC CAACAAGTCG CAGGCTATGG ACTACATTTC GCTGTTAAGT 
CCCCCCAAGC GCAATGTTTT CATGCACCTT TGTATGTTCC TGCGCGCGGG CATCGAGAGT 
CAGTTCTATG ATCTGCATCA AGTCGCATCC ACCTTTGGCC GCATTCTGTT GCGAAGCACC 
GAGCGTGCCG CCTGGATGGA CTACCACAGC CGATGCATAC AGTTCATGCG GCTCTTCATC 
GACACCGACG TCGAGGCAAT GGGCAACGGC AATGAGGGCG CCGGAACTGG AACAGGCTCG 
GGATCTGGGA CCCGGGCGGG TCTGCAGGCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ocrl cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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