Hmt4-20 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hmt4-20
Entrez Gene ID	31015
Full Name	Histone methyltransferase 4-20
Synonyms	BcDNA:LD36415,CG13363,Dmel\CG13363,EG:115C2.10,SUV4-20,Su(var)4-20,Suv4-20,Suv4.20,dSuv4-20,hmt4-20
General protein information	Preferred Names Histone methyltransferase 4-20 Names CG13363 gene product from transcript CG13363-RA CG13363-PA CG13363-PB Hmt4-20-PA Hmt4-20-PB Suv4-20 histone methyltransferase 4-20
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 1B13-1B14

mRNA and Protein(s)

mRNA	Protein	Name
NM_001258524.1	NP_001245453.1	histone methyltransferase 4-20, isoform B
NM_130497.4	NP_569853.1	histone methyltransferase 4-20, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The data indicate that Suv4-20 generates nearly ubiquitous dimethylation that facilitates the DNA damage response and selective trimethylation that is involved in heterochromatin formation.
Title: Preferential dimethylation of histone H4 lysine 20 by Suv4-20.

The following Hmt4-20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hmt4-20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00393	NM_130497.4 Latest version!	Drosophila melanogaster histone methyltransferase 4-20, transcript variant A (Hmt4-20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa00393	NM_001258524.1 Latest version!	Drosophila melanogaster histone methyltransferase 4-20, transcript variant B (Hmt4-20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hmt4-20 ( NM_001258524.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245453.1 Drosophila melanogaster histone methyltransferase 4-20, transcript variant B (Hmt4-20), mRNA.

Price & Availability↑

CloneID	OFa00393
Clone ID Related Accession (Same CDS sequence)	NM_001258524.1 , NM_130497.4
Accession Version	NM_001258524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	histone methyltransferase 4-20, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCGTCG GCTCTAACCA CACGCGGCGC GGTGAAACTG GCAGCAGATT TACAAACAGC 
AGCAGCTCCA GCAGCACAAG CGGTGGCCCC ACCGCTTCGG CCAGTAGCAC CACCAGCGTA 
ACGTCTTCAC TAGCCACCAA TTCCACGTCG ACATCGACGG CTGCGGCTCT GTTGTCGTCC 
ATGTCCCACA AGAGCCATGG CCCTCCGCCA TCCGCACCTC CATCTGCACA CCACCAGACG 
AACCAGCAGC ACCACCAGGT GGCCCACAGC CAGCCACATG CCACCCATTA CCAACAGACA 
AACCAGCACC CTAGCCACCA TCACCAGTCC CATCAGTCTT CGAACGGCAG TGGCGGAGGA 
TCTGCTGGAT CGGGATCTGG ATCTGGATCG GTGGTTAGTG GACTCAATGG TTGCAACGGC 
AGCGCCGTCA GCCGCCTATC GCAATCCACT GGGATGTCGC CGCGGGAACT GTCCGAGAAC 
GATGACCTGG CCACATCGCT GATCCTCGAT CCGCATCTTG GATTTCAGAC CCACAAGATG 
AACATACGCT TTCGGCCTCT TAAGGTGGAC ACGCAACAAC TGAAAGCGAT CGTGGACGAC 
TTTATCCACA CGCAGAACTA CGATATAGCC ATCCAGCGCA TATACGAGGG GCCGTGGATA 
CCGCGTCATC TCAAGAACAA GAACAAGATT GCCACCAAGC GGCTCCACGA TCATATCGTC 
CGATACCTGC GCGTCTTTGA CAAGGATAGC GGCTTTGCAA TCGAGGCCTG CTACAGATAC 
ACACTGGAAG AGCAACGGGG CGCCAAGATT AGCTCGACGA AGCGCTGGTC GAAGAACGAC 
AAGATCGAAT GCCTGGTTGG CTGCATCGCC GAGCTGACAG AGGCGGAGGA GGCCGCTCTG 
CTGCACTCTG GCAAAAATGA CTTCTCAGTA ATGTACAGTT GTCGCAAGAA CTGCGCCCAA 
TTGTGGCTGG GACCGGCTGC CTATATCAAC CACGATTGTC GCGCCAACTG CAAGTTTTTG 
GCCACCGGCA GGGACACAGC GTGTGTAAAG GTCCTGCGCG ACATTGAGGT AGGGGAGGAG 
ATAACCTGCT TCTACGGCGA GGACTTCTTT GGGGATTCCA ATCGCTATTG TGAGTGCGAA 
ACGTGCGAAC GCCGTGGGAC TGGAGCCTTC GCCGGGAAAG ATGATGGCCT GATGCTGGGG 
CTCAGTATGG GACTCGGCTT GGCCAGCAGT GGACCTGGTA ATAATGGCGG CTATCGTCTG 
AGGGAGACAG ACAATCGGAT TAACCGCATC AAGAGCCGAG CCAACTCCAC AAACAGCACC 
TCAAACAGCA ACAGTAATAC AAATGATTCC ACTGGCCCTA GCGAGACTAG TAGCACAAAT 
GGATTGGTGG CTTCTGGCGG AGCAGGAGGA GCCACTGGTG CCGCGATGCT GCCCACACCC 
TCGCAACAAT CAACTGGCGG CAAGGAGGCC ACAGCCGCCG TCTCTTTGCT CGAAAAGAAA 
CTTCCGAATG TTGTTGTTTC ACCGCTGACC ATGAAGGAGC TGCGCCAAAA GGGAATGACT 
AAGTACGATG CCGAGATGAT CATGGCCAAC GCAGCTTACC AGCAGCAGCA CCATCATCAG 
CATCATTTCC ATCACCACCA TCATCATCAT CACCATCACC ACAATCATGG CCAGCATGCA 
AGTACTGGAG CCGAGGCTAC AGCGGCTGTA CAGCAAATGG CGGCCATGCA GAAGCCAGGA 
GTTGGCGGAA CAGGAGCTGC AGGAAATGCT GGAGCAACGA CAGTGAGCAG CGTGGCAGCC 
GGAGCGGGCT CCGAGGTTAA TGGTGGTCGT TCCACCAGTC TACGCAAGTC TATGCGCGTT 
AACAGCACCA GCAGCAGCAT TTCCACCGCC TCCGCGGACG AGGTGATCGC ACCCGTGGTC 
GCGGCGAGCA TTTCGCTGCC CAGCAAGGCA CCGGTGGTTC TTATGCCGCG TTGCAAGCCA 
GCTCAGATGG CCATTGCTGC GCTGCACCAG AGCCAGCAGC GCCAGCTTAG ACGTAGCGAA 
CGCCAGAAGG AAAAGCTGAC GGACGGCGAG AGCAGCGACA CCAGCAGCGA GCAGCAAAAG 
AAAGAGCAGA AGCAGCAGGA TCACCAACTG CCGCAAAAAA TGTTCAGCCT GGCGGAGGAA 
CCACAACCAG AGAAGTCGGA GGAGAAGCAA CAGGAGCAGC AGAAGCGTGT GACGCGAAAT 
AGTGCGGGAA GAGTAGGATT GGTAGCCAGA TTAGCCACTG CCCATAACAA TAACATTGCA 
ACCACAACAA ATAGCAGCAG CAGCAGCAAC AAAGCAACAA CGATTACAAA TTGTAATAAT 
CATAATAGTA ATAATAGCAG TAGAATTAAT CATAATTCAA ATCTTAGCAG TAGACTTAGT 
GTTAAATCGA GGAAGCCAGC ACCAAGTGAG GCCTCGTCCA TACCCTCATC CACATCATCA 
GAAAATCAGC AGCAGCAGGC AACGCGTCGC AGCTGCAGCC CGACTCCCGC GTACAAGAAG 
AACCTTTTGG CCAGCTTTGA TCCCGATCCT CCCTCCACCC AAGGAATCAA GGAGCAGCTA 
AAGGACGAAT CAGTTACTTA TTCGCCGGTC AAGCAGAAAC GTAGCAGAAG GGCCGCTGCT 
CTGGCGGCAG CGCAAAGTAT CCACTGCGAA GCGTTGGGCG GATTCCCTAC CGGTTCAACT 
GGTAGCCAGC GAAAGAGGGC CCAGGCCGGA GAACCTACCA CCTCATGCTC CTCTACAACC 
ATCAGCAATG TTGAGCCCCT ACTGAAGACT CCTGAGCGCA GGCTGAAGCT AACGTTGCGC 
ATGAAACGCT CACCAATCCT AGACGAAGTA ATTGAGCTCG GAACGAGTCT GAGCAACGGA 
GGAGCTGGTC GAGGTGCACC AGGCTCCCAT CGGGAAGGGA CAGCAGGAGA GGGGTCAGTT 
AGAAGTGCGC TAAACCTAAC CGGCAGCAGC TCAAATGGTA TTGAATACGA GATACTGCGC 
ATGGAGGGTA TTTCAGAGCA CGGCAACGAT GACGATGAGG ACGAGGAAGA AGATGATGAA 
GAGCCAGCTG CCGAGGAAGA GGAGGAGCCG CCGCCGAAGG AGGAGCTGCA GCTGGTGAAC 
AAGAAGCAGA GAAAGAAGCA GCGCAGCCGG AGCAGGAGCA GCCAACGCAG ATCGCCGGCC 
CCAAGCAGTG TATATGGAAC ACCGCAGAAG AAGCGACTCC GCTTGATCTT TGGCAACGAA 
TCCCACACCA TAGACATTCC ACCAGCAGCA GCAGAGGGAT CGGGCAGCGG CTTGGATGAC 
CTAAACAGCA GTGGAGGCGG TGGCGACGAG TCCTTCAATG CCTCCTACGC CAGCAGCACC 
AGTCTGACAG TTAATACATC CTCAAGCAGT ACCAGCTCAT CCAGTGGAGT GGGAGGAGCT 
ACGTCCACCT CTGCAGAGCC AGTGGATTCG TCCACGGTTG GACCGCCCAT TGCTGCCCAA 
TCACCTTCAT CGACGACCAG CAGCTCCTTT CAATCGGCCT GCACATCGAC CACCAACAGC 
AACAGCTACT TTCCCAATGG CAAGCAACGA GCTGCTGGCG AGGACTCCTA TGCGATGCAC 
TACTACCAGC TGGGCAAATT TGCTGGAACA CCAAGTCCGG GACAGGGTCA GGCCATCGTG 
AGCAGCAGCA GTGGTTCGTC AGGAGGAGGA GGATCTGGAG CTGGCTTTCT GAGCATGCCC 
AAGCACACAT TCGGCACCTG TGCTCTCCTG GCCCCCACCA GTTTCGCCTG CCTACAGAAT 
CAGCCGCAGA TTAGCCAGCA GAAGACCAGC AGCGGCGGAG GAGCAGGAGT GGTGCCCACC 
TCCACTTCAA CGGGCGCAGT TACATCTCAC CATCATACAA ACAACCATCA CGGCCAGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245453.1
CDS	88..3990
Translation	MVVGSNHTRRGETGSRFTNSSSSSSTSGGPTASASSTTSVTSSLATNSTSTSTAAALLSSMSHKSHGPPPSAPPSAHHQTNQQHHQVAHSQPHATHYQQTNQHPSHHHQSHQSSNGSGGGSAGSGSGSGSVVSGLNGCNGSAVSRLSQSTGMSPRELSENDDLATSLILDPHLGFQTHKMNIRFRPLKVDTQQLKAIVDDFIHTQNYDIAIQRIYEGPWIPRHLKNKNKIATKRLHDHIVRYLRVFDKDSGFAIEACYRYTLEEQRGAKISSTKRWSKNDKIECLVGCIAELTEAEEAALLHSGKNDFSVMYSCRKNCAQLWLGPAAYINHDCRANCKFLATGRDTACVKVLRDIEVGEEITCFYGEDFFGDSNRYCECETCERRGTGAFAGKDDGLMLGLSMGLGLASSGPGNNGGYRLRETDNRINRIKSRANSTNSTSNSNSNTNDSTGPSETSSTNGLVASGGAGGATGAAMLPTPSQQSTGGKEATAAVSLLEKKLPNVVVSPLTMKELRQKGMTKYDAEMIMANAAYQQQHHHQHHFHHHHHHHHHHHNHGQHASTGAEATAAVQQMAAMQKPGVGGTGAAGNAGATTVSSVAAGAGSEVNGGRSTSLRKSMRVNSTSSSISTASADEVIAPVVAASISLPSKAPVVLMPRCKPAQMAIAALHQSQQRQLRRSERQKEKLTDGESSDTSSEQQKKEQKQQDHQLPQKMFSLAEEPQPEKSEEKQQEQQKRVTRNSAGRVGLVARLATAHNNNIATTTNSSSSSNKATTITNCNNHNSNNSSRINHNSNLSSRLSVKSRKPAPSEASSIPSSTSSENQQQQATRRSCSPTPAYKKNLLASFDPDPPSTQGIKEQLKDESVTYSPVKQKRSRRAAALAAAQSIHCEALGGFPTGSTGSQRKRAQAGEPTTSCSSTTISNVEPLLKTPERRLKLTLRMKRSPILDEVIELGTSLSNGGAGRGAPGSHREGTAGEGSVRSALNLTGSSSNGIEYEILRMEGISEHGNDDDEDEEEDDEEPAAEEEEEPPPKEELQLVNKKQRKKQRSRSRSSQRRSPAPSSVYGTPQKKRLRLIFGNESHTIDIPPAAAEGSGSGLDDLNSSGGGGDESFNASYASSTSLTVNTSSSSTSSSSGVGGATSTSAEPVDSSTVGPPIAAQSPSSTTSSSFQSACTSTTNSNSYFPNGKQRAAGEDSYAMHYYQLGKFAGTPSPGQGQAIVSSSSGSSGGGGSGAGFLSMPKHTFGTCALLAPTSFACLQNQPQISQQKTSSGGGAGVVPTSTSTGAVTSHHHTNNHHGQK

Target ORF information:

RefSeq Version	NM_001258524.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster histone methyltransferase 4-20, transcript variant B (Hmt4-20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258524.1

ORF Insert Sequence:

ATGGTCGTCG GCTCTAACCA CACGCGGCGC GGTGAAACTG GCAGCAGATT TACAAACAGC 
AGCAGCTCCA GCAGCACAAG CGGTGGCCCC ACCGCTTCGG CCAGTAGCAC CACCAGCGTA 
ACGTCTTCAC TAGCCACCAA TTCCACGTCG ACATCGACGG CTGCGGCTCT GTTGTCGTCC 
ATGTCCCACA AGAGCCATGG CCCTCCGCCA TCCGCACCTC CATCTGCACA CCACCAGACG 
AACCAGCAGC ACCACCAGGT GGCCCACAGC CAGCCACATG CCACCCATTA CCAACAGACA 
AACCAGCACC CTAGCCACCA TCACCAGTCC CATCAGTCTT CGAACGGCAG TGGCGGAGGA 
TCTGCTGGAT CGGGATCTGG ATCTGGATCG GTGGTTAGTG GACTCAATGG TTGCAACGGC 
AGCGCCGTCA GCCGCCTATC GCAATCCACT GGGATGTCGC CGCGGGAACT GTCCGAGAAC 
GATGACCTGG CCACATCGCT GATCCTCGAT CCGCATCTTG GATTTCAGAC CCACAAGATG 
AACATACGCT TTCGGCCTCT TAAGGTGGAC ACGCAACAAC TGAAAGCGAT CGTGGACGAC 
TTTATCCACA CGCAGAACTA CGATATAGCC ATCCAGCGCA TATACGAGGG GCCGTGGATA 
CCGCGTCATC TCAAGAACAA GAACAAGATT GCCACCAAGC GGCTCCACGA TCATATCGTC 
CGATACCTGC GCGTCTTTGA CAAGGATAGC GGCTTTGCAA TCGAGGCCTG CTACAGATAC 
ACACTGGAAG AGCAACGGGG CGCCAAGATT AGCTCGACGA AGCGCTGGTC GAAGAACGAC 
AAGATCGAAT GCCTGGTTGG CTGCATCGCC GAGCTGACAG AGGCGGAGGA GGCCGCTCTG 
CTGCACTCTG GCAAAAATGA CTTCTCAGTA ATGTACAGTT GTCGCAAGAA CTGCGCCCAA 
TTGTGGCTGG GACCGGCTGC CTATATCAAC CACGATTGTC GCGCCAACTG CAAGTTTTTG 
GCCACCGGCA GGGACACAGC GTGTGTAAAG GTCCTGCGCG ACATTGAGGT AGGGGAGGAG 
ATAACCTGCT TCTACGGCGA GGACTTCTTT GGGGATTCCA ATCGCTATTG TGAGTGCGAA 
ACGTGCGAAC GCCGTGGGAC TGGAGCCTTC GCCGGGAAAG ATGATGGCCT GATGCTGGGG 
CTCAGTATGG GACTCGGCTT GGCCAGCAGT GGACCTGGTA ATAATGGCGG CTATCGTCTG 
AGGGAGACAG ACAATCGGAT TAACCGCATC AAGAGCCGAG CCAACTCCAC AAACAGCACC 
TCAAACAGCA ACAGTAATAC AAATGATTCC ACTGGCCCTA GCGAGACTAG TAGCACAAAT 
GGATTGGTGG CTTCTGGCGG AGCAGGAGGA GCCACTGGTG CCGCGATGCT GCCCACACCC 
TCGCAACAAT CAACTGGCGG CAAGGAGGCC ACAGCCGCCG TCTCTTTGCT CGAAAAGAAA 
CTTCCGAATG TTGTTGTTTC ACCGCTGACC ATGAAGGAGC TGCGCCAAAA GGGAATGACT 
AAGTACGATG CCGAGATGAT CATGGCCAAC GCAGCTTACC AGCAGCAGCA CCATCATCAG 
CATCATTTCC ATCACCACCA TCATCATCAT CACCATCACC ACAATCATGG CCAGCATGCA 
AGTACTGGAG CCGAGGCTAC AGCGGCTGTA CAGCAAATGG CGGCCATGCA GAAGCCAGGA 
GTTGGCGGAA CAGGAGCTGC AGGAAATGCT GGAGCAACGA CAGTGAGCAG CGTGGCAGCC 
GGAGCGGGCT CCGAGGTTAA TGGTGGTCGT TCCACCAGTC TACGCAAGTC TATGCGCGTT 
AACAGCACCA GCAGCAGCAT TTCCACCGCC TCCGCGGACG AGGTGATCGC ACCCGTGGTC 
GCGGCGAGCA TTTCGCTGCC CAGCAAGGCA CCGGTGGTTC TTATGCCGCG TTGCAAGCCA 
GCTCAGATGG CCATTGCTGC GCTGCACCAG AGCCAGCAGC GCCAGCTTAG ACGTAGCGAA 
CGCCAGAAGG AAAAGCTGAC GGACGGCGAG AGCAGCGACA CCAGCAGCGA GCAGCAAAAG 
AAAGAGCAGA AGCAGCAGGA TCACCAACTG CCGCAAAAAA TGTTCAGCCT GGCGGAGGAA 
CCACAACCAG AGAAGTCGGA GGAGAAGCAA CAGGAGCAGC AGAAGCGTGT GACGCGAAAT 
AGTGCGGGAA GAGTAGGATT GGTAGCCAGA TTAGCCACTG CCCATAACAA TAACATTGCA 
ACCACAACAA ATAGCAGCAG CAGCAGCAAC AAAGCAACAA CGATTACAAA TTGTAATAAT 
CATAATAGTA ATAATAGCAG TAGAATTAAT CATAATTCAA ATCTTAGCAG TAGACTTAGT 
GTTAAATCGA GGAAGCCAGC ACCAAGTGAG GCCTCGTCCA TACCCTCATC CACATCATCA 
GAAAATCAGC AGCAGCAGGC AACGCGTCGC AGCTGCAGCC CGACTCCCGC GTACAAGAAG 
AACCTTTTGG CCAGCTTTGA TCCCGATCCT CCCTCCACCC AAGGAATCAA GGAGCAGCTA 
AAGGACGAAT CAGTTACTTA TTCGCCGGTC AAGCAGAAAC GTAGCAGAAG GGCCGCTGCT 
CTGGCGGCAG CGCAAAGTAT CCACTGCGAA GCGTTGGGCG GATTCCCTAC CGGTTCAACT 
GGTAGCCAGC GAAAGAGGGC CCAGGCCGGA GAACCTACCA CCTCATGCTC CTCTACAACC 
ATCAGCAATG TTGAGCCCCT ACTGAAGACT CCTGAGCGCA GGCTGAAGCT AACGTTGCGC 
ATGAAACGCT CACCAATCCT AGACGAAGTA ATTGAGCTCG GAACGAGTCT GAGCAACGGA 
GGAGCTGGTC GAGGTGCACC AGGCTCCCAT CGGGAAGGGA CAGCAGGAGA GGGGTCAGTT 
AGAAGTGCGC TAAACCTAAC CGGCAGCAGC TCAAATGGTA TTGAATACGA GATACTGCGC 
ATGGAGGGTA TTTCAGAGCA CGGCAACGAT GACGATGAGG ACGAGGAAGA AGATGATGAA 
GAGCCAGCTG CCGAGGAAGA GGAGGAGCCG CCGCCGAAGG AGGAGCTGCA GCTGGTGAAC 
AAGAAGCAGA GAAAGAAGCA GCGCAGCCGG AGCAGGAGCA GCCAACGCAG ATCGCCGGCC 
CCAAGCAGTG TATATGGAAC ACCGCAGAAG AAGCGACTCC GCTTGATCTT TGGCAACGAA 
TCCCACACCA TAGACATTCC ACCAGCAGCA GCAGAGGGAT CGGGCAGCGG CTTGGATGAC 
CTAAACAGCA GTGGAGGCGG TGGCGACGAG TCCTTCAATG CCTCCTACGC CAGCAGCACC 
AGTCTGACAG TTAATACATC CTCAAGCAGT ACCAGCTCAT CCAGTGGAGT GGGAGGAGCT 
ACGTCCACCT CTGCAGAGCC AGTGGATTCG TCCACGGTTG GACCGCCCAT TGCTGCCCAA 
TCACCTTCAT CGACGACCAG CAGCTCCTTT CAATCGGCCT GCACATCGAC CACCAACAGC 
AACAGCTACT TTCCCAATGG CAAGCAACGA GCTGCTGGCG AGGACTCCTA TGCGATGCAC 
TACTACCAGC TGGGCAAATT TGCTGGAACA CCAAGTCCGG GACAGGGTCA GGCCATCGTG 
AGCAGCAGCA GTGGTTCGTC AGGAGGAGGA GGATCTGGAG CTGGCTTTCT GAGCATGCCC 
AAGCACACAT TCGGCACCTG TGCTCTCCTG GCCCCCACCA GTTTCGCCTG CCTACAGAAT 
CAGCCGCAGA TTAGCCAGCA GAAGACCAGC AGCGGCGGAG GAGCAGGAGT GGTGCCCACC 
TCCACTTCAA CGGGCGCAGT TACATCTCAC CATCATACAA ACAACCATCA CGGCCAGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hmt4-20 ( NM_130497.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_569853.1 Drosophila melanogaster histone methyltransferase 4-20, transcript variant A (Hmt4-20), mRNA.

Price & Availability↑

CloneID	OFa00393
Clone ID Related Accession (Same CDS sequence)	NM_001258524.1 , NM_130497.4
Accession Version	NM_130497.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	histone methyltransferase 4-20, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_130497.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCGTCG GCTCTAACCA CACGCGGCGC GGTGAAACTG GCAGCAGATT TACAAACAGC 
AGCAGCTCCA GCAGCACAAG CGGTGGCCCC ACCGCTTCGG CCAGTAGCAC CACCAGCGTA 
ACGTCTTCAC TAGCCACCAA TTCCACGTCG ACATCGACGG CTGCGGCTCT GTTGTCGTCC 
ATGTCCCACA AGAGCCATGG CCCTCCGCCA TCCGCACCTC CATCTGCACA CCACCAGACG 
AACCAGCAGC ACCACCAGGT GGCCCACAGC CAGCCACATG CCACCCATTA CCAACAGACA 
AACCAGCACC CTAGCCACCA TCACCAGTCC CATCAGTCTT CGAACGGCAG TGGCGGAGGA 
TCTGCTGGAT CGGGATCTGG ATCTGGATCG GTGGTTAGTG GACTCAATGG TTGCAACGGC 
AGCGCCGTCA GCCGCCTATC GCAATCCACT GGGATGTCGC CGCGGGAACT GTCCGAGAAC 
GATGACCTGG CCACATCGCT GATCCTCGAT CCGCATCTTG GATTTCAGAC CCACAAGATG 
AACATACGCT TTCGGCCTCT TAAGGTGGAC ACGCAACAAC TGAAAGCGAT CGTGGACGAC 
TTTATCCACA CGCAGAACTA CGATATAGCC ATCCAGCGCA TATACGAGGG GCCGTGGATA 
CCGCGTCATC TCAAGAACAA GAACAAGATT GCCACCAAGC GGCTCCACGA TCATATCGTC 
CGATACCTGC GCGTCTTTGA CAAGGATAGC GGCTTTGCAA TCGAGGCCTG CTACAGATAC 
ACACTGGAAG AGCAACGGGG CGCCAAGATT AGCTCGACGA AGCGCTGGTC GAAGAACGAC 
AAGATCGAAT GCCTGGTTGG CTGCATCGCC GAGCTGACAG AGGCGGAGGA GGCCGCTCTG 
CTGCACTCTG GCAAAAATGA CTTCTCAGTA ATGTACAGTT GTCGCAAGAA CTGCGCCCAA 
TTGTGGCTGG GACCGGCTGC CTATATCAAC CACGATTGTC GCGCCAACTG CAAGTTTTTG 
GCCACCGGCA GGGACACAGC GTGTGTAAAG GTCCTGCGCG ACATTGAGGT AGGGGAGGAG 
ATAACCTGCT TCTACGGCGA GGACTTCTTT GGGGATTCCA ATCGCTATTG TGAGTGCGAA 
ACGTGCGAAC GCCGTGGGAC TGGAGCCTTC GCCGGGAAAG ATGATGGCCT GATGCTGGGG 
CTCAGTATGG GACTCGGCTT GGCCAGCAGT GGACCTGGTA ATAATGGCGG CTATCGTCTG 
AGGGAGACAG ACAATCGGAT TAACCGCATC AAGAGCCGAG CCAACTCCAC AAACAGCACC 
TCAAACAGCA ACAGTAATAC AAATGATTCC ACTGGCCCTA GCGAGACTAG TAGCACAAAT 
GGATTGGTGG CTTCTGGCGG AGCAGGAGGA GCCACTGGTG CCGCGATGCT GCCCACACCC 
TCGCAACAAT CAACTGGCGG CAAGGAGGCC ACAGCCGCCG TCTCTTTGCT CGAAAAGAAA 
CTTCCGAATG TTGTTGTTTC ACCGCTGACC ATGAAGGAGC TGCGCCAAAA GGGAATGACT 
AAGTACGATG CCGAGATGAT CATGGCCAAC GCAGCTTACC AGCAGCAGCA CCATCATCAG 
CATCATTTCC ATCACCACCA TCATCATCAT CACCATCACC ACAATCATGG CCAGCATGCA 
AGTACTGGAG CCGAGGCTAC AGCGGCTGTA CAGCAAATGG CGGCCATGCA GAAGCCAGGA 
GTTGGCGGAA CAGGAGCTGC AGGAAATGCT GGAGCAACGA CAGTGAGCAG CGTGGCAGCC 
GGAGCGGGCT CCGAGGTTAA TGGTGGTCGT TCCACCAGTC TACGCAAGTC TATGCGCGTT 
AACAGCACCA GCAGCAGCAT TTCCACCGCC TCCGCGGACG AGGTGATCGC ACCCGTGGTC 
GCGGCGAGCA TTTCGCTGCC CAGCAAGGCA CCGGTGGTTC TTATGCCGCG TTGCAAGCCA 
GCTCAGATGG CCATTGCTGC GCTGCACCAG AGCCAGCAGC GCCAGCTTAG ACGTAGCGAA 
CGCCAGAAGG AAAAGCTGAC GGACGGCGAG AGCAGCGACA CCAGCAGCGA GCAGCAAAAG 
AAAGAGCAGA AGCAGCAGGA TCACCAACTG CCGCAAAAAA TGTTCAGCCT GGCGGAGGAA 
CCACAACCAG AGAAGTCGGA GGAGAAGCAA CAGGAGCAGC AGAAGCGTGT GACGCGAAAT 
AGTGCGGGAA GAGTAGGATT GGTAGCCAGA TTAGCCACTG CCCATAACAA TAACATTGCA 
ACCACAACAA ATAGCAGCAG CAGCAGCAAC AAAGCAACAA CGATTACAAA TTGTAATAAT 
CATAATAGTA ATAATAGCAG TAGAATTAAT CATAATTCAA ATCTTAGCAG TAGACTTAGT 
GTTAAATCGA GGAAGCCAGC ACCAAGTGAG GCCTCGTCCA TACCCTCATC CACATCATCA 
GAAAATCAGC AGCAGCAGGC AACGCGTCGC AGCTGCAGCC CGACTCCCGC GTACAAGAAG 
AACCTTTTGG CCAGCTTTGA TCCCGATCCT CCCTCCACCC AAGGAATCAA GGAGCAGCTA 
AAGGACGAAT CAGTTACTTA TTCGCCGGTC AAGCAGAAAC GTAGCAGAAG GGCCGCTGCT 
CTGGCGGCAG CGCAAAGTAT CCACTGCGAA GCGTTGGGCG GATTCCCTAC CGGTTCAACT 
GGTAGCCAGC GAAAGAGGGC CCAGGCCGGA GAACCTACCA CCTCATGCTC CTCTACAACC 
ATCAGCAATG TTGAGCCCCT ACTGAAGACT CCTGAGCGCA GGCTGAAGCT AACGTTGCGC 
ATGAAACGCT CACCAATCCT AGACGAAGTA ATTGAGCTCG GAACGAGTCT GAGCAACGGA 
GGAGCTGGTC GAGGTGCACC AGGCTCCCAT CGGGAAGGGA CAGCAGGAGA GGGGTCAGTT 
AGAAGTGCGC TAAACCTAAC CGGCAGCAGC TCAAATGGTA TTGAATACGA GATACTGCGC 
ATGGAGGGTA TTTCAGAGCA CGGCAACGAT GACGATGAGG ACGAGGAAGA AGATGATGAA 
GAGCCAGCTG CCGAGGAAGA GGAGGAGCCG CCGCCGAAGG AGGAGCTGCA GCTGGTGAAC 
AAGAAGCAGA GAAAGAAGCA GCGCAGCCGG AGCAGGAGCA GCCAACGCAG ATCGCCGGCC 
CCAAGCAGTG TATATGGAAC ACCGCAGAAG AAGCGACTCC GCTTGATCTT TGGCAACGAA 
TCCCACACCA TAGACATTCC ACCAGCAGCA GCAGAGGGAT CGGGCAGCGG CTTGGATGAC 
CTAAACAGCA GTGGAGGCGG TGGCGACGAG TCCTTCAATG CCTCCTACGC CAGCAGCACC 
AGTCTGACAG TTAATACATC CTCAAGCAGT ACCAGCTCAT CCAGTGGAGT GGGAGGAGCT 
ACGTCCACCT CTGCAGAGCC AGTGGATTCG TCCACGGTTG GACCGCCCAT TGCTGCCCAA 
TCACCTTCAT CGACGACCAG CAGCTCCTTT CAATCGGCCT GCACATCGAC CACCAACAGC 
AACAGCTACT TTCCCAATGG CAAGCAACGA GCTGCTGGCG AGGACTCCTA TGCGATGCAC 
TACTACCAGC TGGGCAAATT TGCTGGAACA CCAAGTCCGG GACAGGGTCA GGCCATCGTG 
AGCAGCAGCA GTGGTTCGTC AGGAGGAGGA GGATCTGGAG CTGGCTTTCT GAGCATGCCC 
AAGCACACAT TCGGCACCTG TGCTCTCCTG GCCCCCACCA GTTTCGCCTG CCTACAGAAT 
CAGCCGCAGA TTAGCCAGCA GAAGACCAGC AGCGGCGGAG GAGCAGGAGT GGTGCCCACC 
TCCACTTCAA CGGGCGCAGT TACATCTCAC CATCATACAA ACAACCATCA CGGCCAGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569853.1
CDS	668..4570
Translation	MVVGSNHTRRGETGSRFTNSSSSSSTSGGPTASASSTTSVTSSLATNSTSTSTAAALLSSMSHKSHGPPPSAPPSAHHQTNQQHHQVAHSQPHATHYQQTNQHPSHHHQSHQSSNGSGGGSAGSGSGSGSVVSGLNGCNGSAVSRLSQSTGMSPRELSENDDLATSLILDPHLGFQTHKMNIRFRPLKVDTQQLKAIVDDFIHTQNYDIAIQRIYEGPWIPRHLKNKNKIATKRLHDHIVRYLRVFDKDSGFAIEACYRYTLEEQRGAKISSTKRWSKNDKIECLVGCIAELTEAEEAALLHSGKNDFSVMYSCRKNCAQLWLGPAAYINHDCRANCKFLATGRDTACVKVLRDIEVGEEITCFYGEDFFGDSNRYCECETCERRGTGAFAGKDDGLMLGLSMGLGLASSGPGNNGGYRLRETDNRINRIKSRANSTNSTSNSNSNTNDSTGPSETSSTNGLVASGGAGGATGAAMLPTPSQQSTGGKEATAAVSLLEKKLPNVVVSPLTMKELRQKGMTKYDAEMIMANAAYQQQHHHQHHFHHHHHHHHHHHNHGQHASTGAEATAAVQQMAAMQKPGVGGTGAAGNAGATTVSSVAAGAGSEVNGGRSTSLRKSMRVNSTSSSISTASADEVIAPVVAASISLPSKAPVVLMPRCKPAQMAIAALHQSQQRQLRRSERQKEKLTDGESSDTSSEQQKKEQKQQDHQLPQKMFSLAEEPQPEKSEEKQQEQQKRVTRNSAGRVGLVARLATAHNNNIATTTNSSSSSNKATTITNCNNHNSNNSSRINHNSNLSSRLSVKSRKPAPSEASSIPSSTSSENQQQQATRRSCSPTPAYKKNLLASFDPDPPSTQGIKEQLKDESVTYSPVKQKRSRRAAALAAAQSIHCEALGGFPTGSTGSQRKRAQAGEPTTSCSSTTISNVEPLLKTPERRLKLTLRMKRSPILDEVIELGTSLSNGGAGRGAPGSHREGTAGEGSVRSALNLTGSSSNGIEYEILRMEGISEHGNDDDEDEEEDDEEPAAEEEEEPPPKEELQLVNKKQRKKQRSRSRSSQRRSPAPSSVYGTPQKKRLRLIFGNESHTIDIPPAAAEGSGSGLDDLNSSGGGGDESFNASYASSTSLTVNTSSSSTSSSSGVGGATSTSAEPVDSSTVGPPIAAQSPSSTTSSSFQSACTSTTNSNSYFPNGKQRAAGEDSYAMHYYQLGKFAGTPSPGQGQAIVSSSSGSSGGGGSGAGFLSMPKHTFGTCALLAPTSFACLQNQPQISQQKTSSGGGAGVVPTSTSTGAVTSHHHTNNHHGQK

Target ORF information:

RefSeq Version	NM_130497.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster histone methyltransferase 4-20, transcript variant A (Hmt4-20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130497.4

ORF Insert Sequence:

ATGGTCGTCG GCTCTAACCA CACGCGGCGC GGTGAAACTG GCAGCAGATT TACAAACAGC 
AGCAGCTCCA GCAGCACAAG CGGTGGCCCC ACCGCTTCGG CCAGTAGCAC CACCAGCGTA 
ACGTCTTCAC TAGCCACCAA TTCCACGTCG ACATCGACGG CTGCGGCTCT GTTGTCGTCC 
ATGTCCCACA AGAGCCATGG CCCTCCGCCA TCCGCACCTC CATCTGCACA CCACCAGACG 
AACCAGCAGC ACCACCAGGT GGCCCACAGC CAGCCACATG CCACCCATTA CCAACAGACA 
AACCAGCACC CTAGCCACCA TCACCAGTCC CATCAGTCTT CGAACGGCAG TGGCGGAGGA 
TCTGCTGGAT CGGGATCTGG ATCTGGATCG GTGGTTAGTG GACTCAATGG TTGCAACGGC 
AGCGCCGTCA GCCGCCTATC GCAATCCACT GGGATGTCGC CGCGGGAACT GTCCGAGAAC 
GATGACCTGG CCACATCGCT GATCCTCGAT CCGCATCTTG GATTTCAGAC CCACAAGATG 
AACATACGCT TTCGGCCTCT TAAGGTGGAC ACGCAACAAC TGAAAGCGAT CGTGGACGAC 
TTTATCCACA CGCAGAACTA CGATATAGCC ATCCAGCGCA TATACGAGGG GCCGTGGATA 
CCGCGTCATC TCAAGAACAA GAACAAGATT GCCACCAAGC GGCTCCACGA TCATATCGTC 
CGATACCTGC GCGTCTTTGA CAAGGATAGC GGCTTTGCAA TCGAGGCCTG CTACAGATAC 
ACACTGGAAG AGCAACGGGG CGCCAAGATT AGCTCGACGA AGCGCTGGTC GAAGAACGAC 
AAGATCGAAT GCCTGGTTGG CTGCATCGCC GAGCTGACAG AGGCGGAGGA GGCCGCTCTG 
CTGCACTCTG GCAAAAATGA CTTCTCAGTA ATGTACAGTT GTCGCAAGAA CTGCGCCCAA 
TTGTGGCTGG GACCGGCTGC CTATATCAAC CACGATTGTC GCGCCAACTG CAAGTTTTTG 
GCCACCGGCA GGGACACAGC GTGTGTAAAG GTCCTGCGCG ACATTGAGGT AGGGGAGGAG 
ATAACCTGCT TCTACGGCGA GGACTTCTTT GGGGATTCCA ATCGCTATTG TGAGTGCGAA 
ACGTGCGAAC GCCGTGGGAC TGGAGCCTTC GCCGGGAAAG ATGATGGCCT GATGCTGGGG 
CTCAGTATGG GACTCGGCTT GGCCAGCAGT GGACCTGGTA ATAATGGCGG CTATCGTCTG 
AGGGAGACAG ACAATCGGAT TAACCGCATC AAGAGCCGAG CCAACTCCAC AAACAGCACC 
TCAAACAGCA ACAGTAATAC AAATGATTCC ACTGGCCCTA GCGAGACTAG TAGCACAAAT 
GGATTGGTGG CTTCTGGCGG AGCAGGAGGA GCCACTGGTG CCGCGATGCT GCCCACACCC 
TCGCAACAAT CAACTGGCGG CAAGGAGGCC ACAGCCGCCG TCTCTTTGCT CGAAAAGAAA 
CTTCCGAATG TTGTTGTTTC ACCGCTGACC ATGAAGGAGC TGCGCCAAAA GGGAATGACT 
AAGTACGATG CCGAGATGAT CATGGCCAAC GCAGCTTACC AGCAGCAGCA CCATCATCAG 
CATCATTTCC ATCACCACCA TCATCATCAT CACCATCACC ACAATCATGG CCAGCATGCA 
AGTACTGGAG CCGAGGCTAC AGCGGCTGTA CAGCAAATGG CGGCCATGCA GAAGCCAGGA 
GTTGGCGGAA CAGGAGCTGC AGGAAATGCT GGAGCAACGA CAGTGAGCAG CGTGGCAGCC 
GGAGCGGGCT CCGAGGTTAA TGGTGGTCGT TCCACCAGTC TACGCAAGTC TATGCGCGTT 
AACAGCACCA GCAGCAGCAT TTCCACCGCC TCCGCGGACG AGGTGATCGC ACCCGTGGTC 
GCGGCGAGCA TTTCGCTGCC CAGCAAGGCA CCGGTGGTTC TTATGCCGCG TTGCAAGCCA 
GCTCAGATGG CCATTGCTGC GCTGCACCAG AGCCAGCAGC GCCAGCTTAG ACGTAGCGAA 
CGCCAGAAGG AAAAGCTGAC GGACGGCGAG AGCAGCGACA CCAGCAGCGA GCAGCAAAAG 
AAAGAGCAGA AGCAGCAGGA TCACCAACTG CCGCAAAAAA TGTTCAGCCT GGCGGAGGAA 
CCACAACCAG AGAAGTCGGA GGAGAAGCAA CAGGAGCAGC AGAAGCGTGT GACGCGAAAT 
AGTGCGGGAA GAGTAGGATT GGTAGCCAGA TTAGCCACTG CCCATAACAA TAACATTGCA 
ACCACAACAA ATAGCAGCAG CAGCAGCAAC AAAGCAACAA CGATTACAAA TTGTAATAAT 
CATAATAGTA ATAATAGCAG TAGAATTAAT CATAATTCAA ATCTTAGCAG TAGACTTAGT 
GTTAAATCGA GGAAGCCAGC ACCAAGTGAG GCCTCGTCCA TACCCTCATC CACATCATCA 
GAAAATCAGC AGCAGCAGGC AACGCGTCGC AGCTGCAGCC CGACTCCCGC GTACAAGAAG 
AACCTTTTGG CCAGCTTTGA TCCCGATCCT CCCTCCACCC AAGGAATCAA GGAGCAGCTA 
AAGGACGAAT CAGTTACTTA TTCGCCGGTC AAGCAGAAAC GTAGCAGAAG GGCCGCTGCT 
CTGGCGGCAG CGCAAAGTAT CCACTGCGAA GCGTTGGGCG GATTCCCTAC CGGTTCAACT 
GGTAGCCAGC GAAAGAGGGC CCAGGCCGGA GAACCTACCA CCTCATGCTC CTCTACAACC 
ATCAGCAATG TTGAGCCCCT ACTGAAGACT CCTGAGCGCA GGCTGAAGCT AACGTTGCGC 
ATGAAACGCT CACCAATCCT AGACGAAGTA ATTGAGCTCG GAACGAGTCT GAGCAACGGA 
GGAGCTGGTC GAGGTGCACC AGGCTCCCAT CGGGAAGGGA CAGCAGGAGA GGGGTCAGTT 
AGAAGTGCGC TAAACCTAAC CGGCAGCAGC TCAAATGGTA TTGAATACGA GATACTGCGC 
ATGGAGGGTA TTTCAGAGCA CGGCAACGAT GACGATGAGG ACGAGGAAGA AGATGATGAA 
GAGCCAGCTG CCGAGGAAGA GGAGGAGCCG CCGCCGAAGG AGGAGCTGCA GCTGGTGAAC 
AAGAAGCAGA GAAAGAAGCA GCGCAGCCGG AGCAGGAGCA GCCAACGCAG ATCGCCGGCC 
CCAAGCAGTG TATATGGAAC ACCGCAGAAG AAGCGACTCC GCTTGATCTT TGGCAACGAA 
TCCCACACCA TAGACATTCC ACCAGCAGCA GCAGAGGGAT CGGGCAGCGG CTTGGATGAC 
CTAAACAGCA GTGGAGGCGG TGGCGACGAG TCCTTCAATG CCTCCTACGC CAGCAGCACC 
AGTCTGACAG TTAATACATC CTCAAGCAGT ACCAGCTCAT CCAGTGGAGT GGGAGGAGCT 
ACGTCCACCT CTGCAGAGCC AGTGGATTCG TCCACGGTTG GACCGCCCAT TGCTGCCCAA 
TCACCTTCAT CGACGACCAG CAGCTCCTTT CAATCGGCCT GCACATCGAC CACCAACAGC 
AACAGCTACT TTCCCAATGG CAAGCAACGA GCTGCTGGCG AGGACTCCTA TGCGATGCAC 
TACTACCAGC TGGGCAAATT TGCTGGAACA CCAAGTCCGG GACAGGGTCA GGCCATCGTG 
AGCAGCAGCA GTGGTTCGTC AGGAGGAGGA GGATCTGGAG CTGGCTTTCT GAGCATGCCC 
AAGCACACAT TCGGCACCTG TGCTCTCCTG GCCCCCACCA GTTTCGCCTG CCTACAGAAT 
CAGCCGCAGA TTAGCCAGCA GAAGACCAGC AGCGGCGGAG GAGCAGGAGT GGTGCCCACC 
TCCACTTCAA CGGGCGCAGT TACATCTCAC CATCATACAA ACAACCATCA CGGCCAGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Hmt4-20 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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