sprt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	sprt
Entrez Gene ID	246397
Full Name	sprite
Synonyms	CG30023,CG9026,Dmel\CG30023
General protein information	Preferred Names sprite Names CG30023 gene product from transcript CG30023-RA CG30023-PA sprt-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 47F8-47F8

mRNA and Protein(s)

mRNA	Protein	Name
NM_136839.3	NP_610683.1	sprite

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	sprt	NP_610683.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005235	XP_314138.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sprt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sprt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11562	NM_136839.3 Latest version!	Drosophila melanogaster sprite (sprt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sprt ( NM_136839.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610683.1 Drosophila melanogaster sprite (sprt), mRNA.

Price & Availability↑

CloneID	OFa11562
Clone ID Related Accession (Same CDS sequence)	NM_136839.3
Accession Version	NM_136839.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sprite
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136839.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACACCG GAAGTGTGGT TAGTGAACCG ATTTCCGCGC ATCATCGCGC CTTCGCCAAA 
CAGAAGTCCG CCTCCATGAC CATTCTCAAT CCGAAGGGTT GTGGCAGTGC CGTCAAGTAC 
AGTCTGCACT CGACTGCAGA GACGAACCAG GAGACTGCCA GTACCACGAC CACCTCTACC 
CATTGTTCCA CAACTGGAAG AAGGCGCAAG GGTGGCTCCC TGGGCGGTAC TCTCTCCTCG 
CGATCGACGC GCAGCGAATC CAAGGAATTG CGATCGGCTC TTCAGGATCG GGAGGCTGTC 
ATCCAGAATC TGCGTATACA GCTTTGTCTG GGCAAACTGC CACGTCCCAC GGGACCACCG 
CTGGATGAGT CCGAGAAGCC GGCGGCTGAG CAAAAACTAA ACAGACTGAA GACGGAGGCG 
GAGAATAAGC GAATTAAGAT CAAGAACCTG AAGAGTGCCC TGGAGAAGCT GGACATCACA 
GACAACATAG ACATCCGCAT CCGTCAAGCC GAATTGGAAT ACGCACTCGG CAGAGAAGAA 
CTCCAGATGC TCTCGATTGT GGAGGAGGCT CGCGCCTTGC AGGCACGTCT GGAAAAGTCC 
AAGCCCGAGG CGCAGACCCT CTACAACATC ATCAGCTCTG GAGTCTCCCT GAGTCTGCAC 
GCCGTGCATG CCACCTCGGG AAGATGGGCA GTTCAACAGA GGACAGACCA GCCGGGTTTC 
TACGTAGAAT GGGCTTTAGA GGGTGATGGC TTGTACAAGG GCGATCGCAT TTTGGAGATC 
AACGGACGAC TGGTAACCGG AAAATCCAAG GAAGAGCTGC AGAAGCAAGT AGGAAACTCT 
GGCAAGTGTC AGATGGTTGT ACTCCGCAAG AAGTCCGTAC CTATACCACA AAAGCAACTA 
GACCAGGAGA AGGAGAACAA CATGAGGCTG CAGCATCGCA TATCGTACCT GGAAGAGCAG 
GTGCGAGAAC TGCAGACAGT CAAGGAGCAT CATCCATCGC AACAGCAGCA GCAGCAGCAG 
CAGCAACAGC AAAATCCACA GCAAAATCCA CAGCAAAATC CACAGCAGAC ATCCTCCAAT 
CAATCTCAGC ATGTGACCAG CATCAGCATA TCATCACCTC CATCAACACC GCCCGAGAAA 
CCGTTGATCT TTCAGCGGGG CAACTATATT ACCACTTTGG TGGGGGGCAA ACCCATCGAG 
CTGATGGGTG ACGACATGGC CTCGACACAA ACCCCTCCCG GACATGTAAC CAAAACACTG 
ATCAAGGAGA ATACCCACAT CGATTTGAGA GATCGTCTGT CGCACATGTA CTCCATGCCC 
TCCAAATCGC TGTCCGCCTC GAAGATCTCG ATTAATAGCG ATTCTGCCTA TATGCAGCAC 
CATCGACGTG ATAAGGAGCG CCATCGCGAT CGCCAGCACC GGGAACGCCC CAGAGATCCT 
CTCCAGCAAC AGCAGCAACT GCATCGCGAA CATCTCATGA GCACGCACGC ACGCAGTGTA 
GAGCACCTGA ATCATTACAA TGGACTGGAT CGTCGCCGAG ACACGCCCCG ACAAAGTGAG 
TCGAACACAC TTCGAGCCCG CCTGCCCAGC GATATGAGGA GTGTAAAATC CCTGGATTTC 
GATAGCGAAA ACGAATCGAA GCCTGCGACT GAGACGCGTA CCACTAGACC CACTCCGCCC 
AAGAAACCCC TCCGGCTATC GCTGCAACGA GCCCAAAGTC TGCAGACCGT GGAGCTGCAC 
CCCTGTGCGG ATATCGAACG AAAGCGGCCC ATGAAGAGGG TCCACCACAA CAACAACAAC 
AACAACAAAG GTGGAGAATC ACCGACTGGA GAGGGCCAGA CCATGCTCAT CGAGAACTGC 
AGTCCCATGC ACACGGCTTC CCTCGGCAGG CACAAGCTCA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610683.1
CDS	573..2477
Translation	MDTGSVVSEPISAHHRAFAKQKSASMTILNPKGCGSAVKYSLHSTAETNQETASTTTTSTHCSTTGRRRKGGSLGGTLSSRSTRSESKELRSALQDREAVIQNLRIQLCLGKLPRPTGPPLDESEKPAAEQKLNRLKTEAENKRIKIKNLKSALEKLDITDNIDIRIRQAELEYALGREELQMLSIVEEARALQARLEKSKPEAQTLYNIISSGVSLSLHAVHATSGRWAVQQRTDQPGFYVEWALEGDGLYKGDRILEINGRLVTGKSKEELQKQVGNSGKCQMVVLRKKSVPIPQKQLDQEKENNMRLQHRISYLEEQVRELQTVKEHHPSQQQQQQQQQQQNPQQNPQQNPQQTSSNQSQHVTSISISSPPSTPPEKPLIFQRGNYITTLVGGKPIELMGDDMASTQTPPGHVTKTLIKENTHIDLRDRLSHMYSMPSKSLSASKISINSDSAYMQHHRRDKERHRDRQHRERPRDPLQQQQQLHREHLMSTHARSVEHLNHYNGLDRRRDTPRQSESNTLRARLPSDMRSVKSLDFDSENESKPATETRTTRPTPPKKPLRLSLQRAQSLQTVELHPCADIERKRPMKRVHHNNNNNNKGGESPTGEGQTMLIENCSPMHTASLGRHKLI

Target ORF information:

RefSeq Version	NM_136839.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sprite (sprt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136839.3

ORF Insert Sequence:

ATGGACACCG GAAGTGTGGT TAGTGAACCG ATTTCCGCGC ATCATCGCGC CTTCGCCAAA 
CAGAAGTCCG CCTCCATGAC CATTCTCAAT CCGAAGGGTT GTGGCAGTGC CGTCAAGTAC 
AGTCTGCACT CGACTGCAGA GACGAACCAG GAGACTGCCA GTACCACGAC CACCTCTACC 
CATTGTTCCA CAACTGGAAG AAGGCGCAAG GGTGGCTCCC TGGGCGGTAC TCTCTCCTCG 
CGATCGACGC GCAGCGAATC CAAGGAATTG CGATCGGCTC TTCAGGATCG GGAGGCTGTC 
ATCCAGAATC TGCGTATACA GCTTTGTCTG GGCAAACTGC CACGTCCCAC GGGACCACCG 
CTGGATGAGT CCGAGAAGCC GGCGGCTGAG CAAAAACTAA ACAGACTGAA GACGGAGGCG 
GAGAATAAGC GAATTAAGAT CAAGAACCTG AAGAGTGCCC TGGAGAAGCT GGACATCACA 
GACAACATAG ACATCCGCAT CCGTCAAGCC GAATTGGAAT ACGCACTCGG CAGAGAAGAA 
CTCCAGATGC TCTCGATTGT GGAGGAGGCT CGCGCCTTGC AGGCACGTCT GGAAAAGTCC 
AAGCCCGAGG CGCAGACCCT CTACAACATC ATCAGCTCTG GAGTCTCCCT GAGTCTGCAC 
GCCGTGCATG CCACCTCGGG AAGATGGGCA GTTCAACAGA GGACAGACCA GCCGGGTTTC 
TACGTAGAAT GGGCTTTAGA GGGTGATGGC TTGTACAAGG GCGATCGCAT TTTGGAGATC 
AACGGACGAC TGGTAACCGG AAAATCCAAG GAAGAGCTGC AGAAGCAAGT AGGAAACTCT 
GGCAAGTGTC AGATGGTTGT ACTCCGCAAG AAGTCCGTAC CTATACCACA AAAGCAACTA 
GACCAGGAGA AGGAGAACAA CATGAGGCTG CAGCATCGCA TATCGTACCT GGAAGAGCAG 
GTGCGAGAAC TGCAGACAGT CAAGGAGCAT CATCCATCGC AACAGCAGCA GCAGCAGCAG 
CAGCAACAGC AAAATCCACA GCAAAATCCA CAGCAAAATC CACAGCAGAC ATCCTCCAAT 
CAATCTCAGC ATGTGACCAG CATCAGCATA TCATCACCTC CATCAACACC GCCCGAGAAA 
CCGTTGATCT TTCAGCGGGG CAACTATATT ACCACTTTGG TGGGGGGCAA ACCCATCGAG 
CTGATGGGTG ACGACATGGC CTCGACACAA ACCCCTCCCG GACATGTAAC CAAAACACTG 
ATCAAGGAGA ATACCCACAT CGATTTGAGA GATCGTCTGT CGCACATGTA CTCCATGCCC 
TCCAAATCGC TGTCCGCCTC GAAGATCTCG ATTAATAGCG ATTCTGCCTA TATGCAGCAC 
CATCGACGTG ATAAGGAGCG CCATCGCGAT CGCCAGCACC GGGAACGCCC CAGAGATCCT 
CTCCAGCAAC AGCAGCAACT GCATCGCGAA CATCTCATGA GCACGCACGC ACGCAGTGTA 
GAGCACCTGA ATCATTACAA TGGACTGGAT CGTCGCCGAG ACACGCCCCG ACAAAGTGAG 
TCGAACACAC TTCGAGCCCG CCTGCCCAGC GATATGAGGA GTGTAAAATC CCTGGATTTC 
GATAGCGAAA ACGAATCGAA GCCTGCGACT GAGACGCGTA CCACTAGACC CACTCCGCCC 
AAGAAACCCC TCCGGCTATC GCTGCAACGA GCCCAAAGTC TGCAGACCGT GGAGCTGCAC 
CCCTGTGCGG ATATCGAACG AAAGCGGCCC ATGAAGAGGG TCCACCACAA CAACAACAAC 
AACAACAAAG GTGGAGAATC ACCGACTGGA GAGGGCCAGA CCATGCTCAT CGAGAACTGC 
AGTCCCATGC ACACGGCTTC CCTCGGCAGG CACAAGCTCA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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sprt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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