LOC109543170 cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109543170
Entrez Gene ID	109543170
Full Name	delta-1-pyrroline-5-carboxylate synthase
Gene Type	protein-coding
Organism	Dendroctonus ponderosae(mountain pine beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019912742.1	XP_019768301.1	delta-1-pyrroline-5-carboxylate synthase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Quantitative metabolome, proteome and transcriptome analysis of midgut and fat body tissues in the mountain pine beetle, Dendroctonus ponderosae Hopkins, and insights into pheromone biosynthesis.
Keeling CI, Li M, Sandhu HK, Henderson H, Yuen MM, Bohlmann J
Insect biochemistry and molecular biology70170-83(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC109543170 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109543170 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe47772	XM_019912742.1 Latest version!	Dendroctonus ponderosae delta-1-pyrroline-5-carboxylate synthase (LOC109543170), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109543170 ( XM_019912742.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019768301.1 Dendroctonus ponderosae delta-1-pyrroline-5-carboxylate synthase (LOC109543170), mRNA.

Price & Availability↑

CloneID	ODe47772
Clone ID Related Accession (Same CDS sequence)	XM_019912742.1
Accession Version	XM_019912742.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	delta-1-pyrroline-5-carboxylate synthase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017852312.1) and transcript sequence (GDAR01001493.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 376 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTTGGGTA GAAGTGTTAT GAATTCAGCC CCCCGATTCC TCAATCAGGG CTTCAATGGG 
GTTGGATTCT TCAAGATACC AGGCCAATTG GCTGCTTTGA GGCCGGTTTT GTTGGCAAAT 
TGTCGACAGG CCAGCACTAG TCCTAGATTA TGGATTCCAT CACTTTCACA TACCAACTTC 
ACAAAAGGGC TAGCAGAAAA ACGAGAAAAT ACGTTTACTG ACCGAAGCCA GTTGAAGTAT 
GCCCGAAGAC TCGTCGTGAA GCTGGGAAGT GCTGTGATAA CTCGAGAAGA CGAACATGGC 
TTGGCTTTGG GAAGATTGGC TTCCATTGTC GAGCAGGTTG CTGAATGCCA GAACGAAGGA 
AGAGAATGCA TAATGGTGAC CAGTGGTGCC GTTGCTTTCG GCAAACAGAA GCTGACGCAA 
GAATTGCTGA TGTCGCTGTC CATGAGGGAA ACTTTATCTC CAACTGACCA CACAAGAGAA 
GATGCCCCAA CTCTAGTGGA ACCTCGAGCG GCCGCAGCTG TAGGCCAATC GGGTCTAATG 
TCTTTATATG ATGCCATGTT TTCCCAATAT GGAGTCAAAA TTGCCCAAGT TCTAGTAACT 
AAAGCTGATT TCTACAATGA GGAAACTAGA AAGAACATAA TCAGCACTTT ATCTGAGCTG 
ATCAGTTTGA ACATCGTTCC AATCATTAAC ACCAACGACG CTGTATCTCC ACCTCCTCAA 
ATTGATGAAC CAATCGGAGC CAAACCGGGC AAACGAGGCA TTTCTCTGAA AGACAACGAT 
TCTCTGGCAG CGATGTTGGC AGCAGAAATT CAATCCGATT TGTTGATTTT GATGTCTGAT 
GTTGATGGCA TTTACAACAA ACCTCCTTGG GAACCAGGCG CGAGATTCCT TCACACGTTC 
TCTTCTGAAC AAAGGCAGAC CATCGAGTTT GGCCAGAAGA GTAAAGTTGG TACTGGAGGA 
ATGGACTCTA AAGTAAACAG TGCGATTTGG GCTTTGGATA GAGGAGTCTC TGTGGTTATA 
TGCAACGGAA TGGCTAAGGA GGCAGTAAAA ACAATCCTTT CTGGGCGGAA AATCGGCACT 
TTCTTCACAG ACTCTGCAAA CATTGGGGCT GTGCCAACTG AAATTGTTGC AGAAAATGCT 
CGAATTGGAG GTCGCATTCT CCAAACTCTT ACGCCCGAAC AAAGGGCCTC ATGCGTGCAT 
ACGTTGGCCG ATCTTTTGAT CTCCAAACAA TCGCAGATCC TGGACGCCAA TCAAAAGGAT 
CTACAAGAGG CAACTAGCTC TGGATTGGCC AAGCCTTTGT TATCCAGATT GTCGCTCACG 
CCAGCAAAGC TCAAGAATCT AGCAGTTGGG TTGAACCAGA TTGCCGAGTC GAGTCACAAC 
AACGTGGGGC GAGTCCTTAG AAGAACCAAA TTGGCGGAAG GACTAGACCT TAAGCAAATC 
ACTGTTCCCA TTGGCGTCCT TCTAGTCATT TTCGAATCCA GACCTGACAG TTTGCCTCAG 
GTAGCAGCCC TTGCTATTGC CTCAGGGAAT GGACTTTTAC TTAAAGGTGG AAAAGAAGCT 
GCCCACAGCA ACAAAGCTCT TATGGAGCTA GTAAAGGAAG CTTTAAGCAC TATTGGCGCA 
GGAAATGCAA TTTCTTTGGT TTCCACCAGA GAGGAAATTG GAGATCTTTT AAGCATGGAG 
CAACACATCG ATCTGATAAT CCCGAGAGGA TCCAGTGAAT TAGTTAGGAG CATTCAGCAG 
CAGTCCCAGC ACATTCCTGT TCTAGGTCAT GCAGAAGGTA TATGTCACGT TTTTGTTGAC 
AAAGATGCGG ATCTGAAAAA GGCTCTGAAA ATCATCAGGG ACGCCAAATG CGACTATCCA 
GCGGCTTGCA ATGCAATGGA AACTTTGTTG GTCCATGAAG ATTTGATGAG CACCAGTTTC 
TTCTCTGATG TATGCGATTT GTTGAAGAAG GAAGGTGTTA CCATTTACTC AGGTCCCGTA 
TTAAACCAGA TGCTCACTTT TGGACCCCCA TTGGCCAAGA GCATGAAGCA TGAATACGGA 
GCTCTCCAAT GCTGCATTGA AGTTGTAAAA GGCTTTGATG AAGCTATTGA ACACATTCAT 
ACTTATGGCA GCGGACACAC TGATGTTATT GTCACTGAAA ACAATAGTAG AGCAGAAGAC 
TTCCAAAAGA AAGTGGACAG CGCCTGTGTA TTCCACAATG CCAGCTCTCG GTTTGCTGAT 
GGGTTCCGAT TCGGCTTAGG TGCTGAGGTA GGAATTAGTA CTGCCAGGAT TCACGCACGA 
GGACCTGTAG GAGTTGAGGG ACTGCTGACC ACCAAATGGG TGCTAGATGG CGTTGATCAT 
GCAGCTTCGG ACTTTTCTGA AGAAGGTGGC CGCAAATGGC TACACGAATC GTTGCCTGTA 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019768301.1
CDS	517..2922
Translation	MLGRSVMNSAPRFLNQGFNGVGFFKIPGQLAALRPVLLANCRQASTSPRLWIPSLSHTNFTKGLAEKRENTFTDRSQLKYARRLVVKLGSAVITREDEHGLALGRLASIVEQVAECQNEGRECIMVTSGAVAFGKQKLTQELLMSLSMRETLSPTDHTREDAPTLVEPRAAAAVGQSGLMSLYDAMFSQYGVKIAQVLVTKADFYNEETRKNIISTLSELISLNIVPIINTNDAVSPPPQIDEPIGAKPGKRGISLKDNDSLAAMLAAEIQSDLLILMSDVDGIYNKPPWEPGARFLHTFSSEQRQTIEFGQKSKVGTGGMDSKVNSAIWALDRGVSVVICNGMAKEAVKTILSGRKIGTFFTDSANIGAVPTEIVAENARIGGRILQTLTPEQRASCVHTLADLLISKQSQILDANQKDLQEATSSGLAKPLLSRLSLTPAKLKNLAVGLNQIAESSHNNVGRVLRRTKLAEGLDLKQITVPIGVLLVIFESRPDSLPQVAALAIASGNGLLLKGGKEAAHSNKALMELVKEALSTIGAGNAISLVSTREEIGDLLSMEQHIDLIIPRGSSELVRSIQQQSQHIPVLGHAEGICHVFVDKDADLKKALKIIRDAKCDYPAACNAMETLLVHEDLMSTSFFSDVCDLLKKEGVTIYSGPVLNQMLTFGPPLAKSMKHEYGALQCCIEVVKGFDEAIEHIHTYGSGHTDVIVTENNSRAEDFQKKVDSACVFHNASSRFADGFRFGLGAEVGISTARIHARGPVGVEGLLTTKWVLDGVDHAASDFSEEGGRKWLHESLPVN

Target ORF information:

RefSeq Version	XM_019912742.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae delta-1-pyrroline-5-carboxylate synthase (LOC109543170), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019912742.1

ORF Insert Sequence:

ATGTTGGGTA GAAGTGTTAT GAATTCAGCC CCCCGATTCC TCAATCAGGG CTTCAATGGG 
GTTGGATTCT TCAAGATACC AGGCCAATTG GCTGCTTTGA GGCCGGTTTT GTTGGCAAAT 
TGTCGACAGG CCAGCACTAG TCCTAGATTA TGGATTCCAT CACTTTCACA TACCAACTTC 
ACAAAAGGGC TAGCAGAAAA ACGAGAAAAT ACGTTTACTG ACCGAAGCCA GTTGAAGTAT 
GCCCGAAGAC TCGTCGTGAA GCTGGGAAGT GCTGTGATAA CTCGAGAAGA CGAACATGGC 
TTGGCTTTGG GAAGATTGGC TTCCATTGTC GAGCAGGTTG CTGAATGCCA GAACGAAGGA 
AGAGAATGCA TAATGGTGAC CAGTGGTGCC GTTGCTTTCG GCAAACAGAA GCTGACGCAA 
GAATTGCTGA TGTCGCTGTC CATGAGGGAA ACTTTATCTC CAACTGACCA CACAAGAGAA 
GATGCCCCAA CTCTAGTGGA ACCTCGAGCG GCCGCAGCTG TAGGCCAATC GGGTCTAATG 
TCTTTATATG ATGCCATGTT TTCCCAATAT GGAGTCAAAA TTGCCCAAGT TCTAGTAACT 
AAAGCTGATT TCTACAATGA GGAAACTAGA AAGAACATAA TCAGCACTTT ATCTGAGCTG 
ATCAGTTTGA ACATCGTTCC AATCATTAAC ACCAACGACG CTGTATCTCC ACCTCCTCAA 
ATTGATGAAC CAATCGGAGC CAAACCGGGC AAACGAGGCA TTTCTCTGAA AGACAACGAT 
TCTCTGGCAG CGATGTTGGC AGCAGAAATT CAATCCGATT TGTTGATTTT GATGTCTGAT 
GTTGATGGCA TTTACAACAA ACCTCCTTGG GAACCAGGCG CGAGATTCCT TCACACGTTC 
TCTTCTGAAC AAAGGCAGAC CATCGAGTTT GGCCAGAAGA GTAAAGTTGG TACTGGAGGA 
ATGGACTCTA AAGTAAACAG TGCGATTTGG GCTTTGGATA GAGGAGTCTC TGTGGTTATA 
TGCAACGGAA TGGCTAAGGA GGCAGTAAAA ACAATCCTTT CTGGGCGGAA AATCGGCACT 
TTCTTCACAG ACTCTGCAAA CATTGGGGCT GTGCCAACTG AAATTGTTGC AGAAAATGCT 
CGAATTGGAG GTCGCATTCT CCAAACTCTT ACGCCCGAAC AAAGGGCCTC ATGCGTGCAT 
ACGTTGGCCG ATCTTTTGAT CTCCAAACAA TCGCAGATCC TGGACGCCAA TCAAAAGGAT 
CTACAAGAGG CAACTAGCTC TGGATTGGCC AAGCCTTTGT TATCCAGATT GTCGCTCACG 
CCAGCAAAGC TCAAGAATCT AGCAGTTGGG TTGAACCAGA TTGCCGAGTC GAGTCACAAC 
AACGTGGGGC GAGTCCTTAG AAGAACCAAA TTGGCGGAAG GACTAGACCT TAAGCAAATC 
ACTGTTCCCA TTGGCGTCCT TCTAGTCATT TTCGAATCCA GACCTGACAG TTTGCCTCAG 
GTAGCAGCCC TTGCTATTGC CTCAGGGAAT GGACTTTTAC TTAAAGGTGG AAAAGAAGCT 
GCCCACAGCA ACAAAGCTCT TATGGAGCTA GTAAAGGAAG CTTTAAGCAC TATTGGCGCA 
GGAAATGCAA TTTCTTTGGT TTCCACCAGA GAGGAAATTG GAGATCTTTT AAGCATGGAG 
CAACACATCG ATCTGATAAT CCCGAGAGGA TCCAGTGAAT TAGTTAGGAG CATTCAGCAG 
CAGTCCCAGC ACATTCCTGT TCTAGGTCAT GCAGAAGGTA TATGTCACGT TTTTGTTGAC 
AAAGATGCGG ATCTGAAAAA GGCTCTGAAA ATCATCAGGG ACGCCAAATG CGACTATCCA 
GCGGCTTGCA ATGCAATGGA AACTTTGTTG GTCCATGAAG ATTTGATGAG CACCAGTTTC 
TTCTCTGATG TATGCGATTT GTTGAAGAAG GAAGGTGTTA CCATTTACTC AGGTCCCGTA 
TTAAACCAGA TGCTCACTTT TGGACCCCCA TTGGCCAAGA GCATGAAGCA TGAATACGGA 
GCTCTCCAAT GCTGCATTGA AGTTGTAAAA GGCTTTGATG AAGCTATTGA ACACATTCAT 
ACTTATGGCA GCGGACACAC TGATGTTATT GTCACTGAAA ACAATAGTAG AGCAGAAGAC 
TTCCAAAAGA AAGTGGACAG CGCCTGTGTA TTCCACAATG CCAGCTCTCG GTTTGCTGAT 
GGGTTCCGAT TCGGCTTAGG TGCTGAGGTA GGAATTAGTA CTGCCAGGAT TCACGCACGA 
GGACCTGTAG GAGTTGAGGG ACTGCTGACC ACCAAATGGG TGCTAGATGG CGTTGATCAT 
GCAGCTTCGG ACTTTTCTGA AGAAGGTGGC CGCAAATGGC TACACGAATC GTTGCCTGTA 
AACTGA