hspa1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	hspa1b
Entrez Gene ID	798846
Full Name	si:ch211-199o1.2
General protein information	Preferred Names si:ch211-199o1.2 Names uncharacterized protein LOC798846
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021478327.1	XP_021334002.1	uncharacterized protein LOC798846 isoform X1
NM_001100062.1	NP_001093532.1	uncharacterized protein LOC798846

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	zgc:174006	XP_005163979.1
Saccharomyces cerevisiae (baker's yeast)	SSA2	NP_013076.1
Neurospora crassa	NCU09602	XP_963366.1
Canis lupus familiaris (dog)	HSPA1L	XP_005627220.1
Mus musculus (house mouse)	Hspa1l	NP_038586.2
Eremothecium gossypii	AGOS_AFR114W	NP_985661.1
Danio rerio (zebrafish)	si:ch211-199o1.2	NP_001093532.1
Danio rerio (zebrafish)	hsp70	NP_571472.1
Arabidopsis thaliana (thale cress)	Hsp70b	NP_173055.1
Pan troglodytes (chimpanzee)	HSPA1L	XP_001160137.2
Rattus norvegicus (Norway rat)	Hspa1l	NP_997711.1
Saccharomyces cerevisiae (baker's yeast)	SSA1	NP_009396.2
Magnaporthe oryzae (rice blast fungus)	MGG_06958	XP_003709704.1
Homo sapiens (human)	HSPA1L	NP_005518.3
	LOC716633	XP_002803717.1
Bos taurus (cattle)	HSPA1L	NP_001161367.1
Danio rerio (zebrafish)	hsp70.2	XP_003198158.1
Danio rerio (zebrafish)	hsp70l	NP_001107061.1
Kluyveromyces lactis	KLLA0D04224g	XP_453252.1
Schizosaccharomyces pombe (fission yeast)	ssa1	NP_593704.1
Schizosaccharomyces pombe (fission yeast)	ssa2	NP_588421.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The ATPase-dependent chaperoning activity of Hsp90a regulates thick filament formation and integration during skeletal muscle myofibrillogenesis.
Hawkins TA, Haramis AP, Etard C, Prodromou C, Vaughan CK, Ashworth R, Ray S, Behra M, Holder N, Talbot WS, Pearl LH, Str?hle U, Wilson SW
Development (Cambridge, England)135(6)1147-56(2008 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following hspa1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hspa1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31189	NM_001100062.1 Latest version!	Danio rerio heat shock protein family A (Hsp70) member 1B (hspa1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa31189	XM_021478327.1 Latest version!	Danio rerio si:ch211-199o1.2 (si:ch211-199o1.2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hspa1b ( NM_001100062.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001093532.1 Danio rerio heat shock protein family A (Hsp70) member 1B (hspa1b), mRNA.

Price & Availability↑

CloneID	ODa31189
Clone ID Related Accession (Same CDS sequence)	XM_021478327.1 , NM_001100062.1
Accession Version	NM_001100062.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-15
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC798846
Comment	Comment: PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from BX470135.8. On Jul 26, 2007 this sequence version replaced XM_001336136.1. ##Evidence-Data-START## Transcript exon combination :: GFIL01035815.1, EB833577.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA898401, SAMEA898404 [ECO:0000348] ##Evidence-Data-END##

ATGGCTAAAG GTTTGTCTAT TGGCATTGAT CTGGGAACCA CTTACTCCTG TGTTGGAGTG 
TTCCAGCATG GCAAAGTGGA GATCATCGCT AACGATCAAG GAAACCGAAC CACTCCAAGC 
TATGTGGCTT TTACAGACAC AGAAAGACTC ATCGGAGATG CTGCCAAAAA CCAAGTCGCC 
ATGAACCCCA ACAACACCAT CTTTGACGCC AAACGACTGA TTGGCAGAAA GTTTGATGAT 
CAGGTTGTCC AGTCTGACAT GAAGCTTTGG CCATTTAAGG TCATCAGTGA AGATGGCAAA 
CCAAAAGTTC AAGTCGAGTA CAAAGGGGAA AACAAGACAT TCTACCCTGA GGAGATCTCA 
TCTATGGTCC TGGTCAAAAT GAGAGAGATT GCCGAAGCCT ACCTGGGTCA TAGAGTCAAT 
AATGCTGTCA TCACAGTCCC AGCGTACTTC AACGATTCAC AGAGGCAAGC GACCAAAGAC 
GCAGGGGTCA TTGCCGGATT GAACGTGCTG CGAATTATCA ATGAGCCCAC AGCAGCTGCC 
ATCGCCTACG GCCTGGACAA GGGCAGGAAG GGAGAGAGGA ATGTCCTGAT CTTCGACCTC 
GGCGGAGGCA CCTTTGATGT CTCCATCCTG ACGATTGAGG ATGGGATCTT TGAAGTGAAA 
GCCACAGCAG GGGACACTCA TCTGGGTGGA GAGGACTTCG ACAATCGCAT GGTGAAGCAC 
TTTGTTGAAG AGTTTAAGAG GAAGCACAAG AAGGACATCA GTCAGAATAA GAGAGCAGTA 
AGACGGCTGC GCACGGCCTG CGAGAGAGCT AAAAGGACCC TCTCATCTAG CTCTCAGGCT 
AGCATCGAGA TCGATTCCCT CTTTGAAGGC ATCGATTTCT ACACTTCCAT CACTAGGGCT 
CGCTTTGAAG AGCTTAACGC GGAGCTCTTT AGAGGCACGC TGGATCCAGT GGAAAAGGCT 
TTGAGAGATG CCAAAATGGA CAAGTCTCAG ATCCAAGATA TTGTTCTGGT TGGCGGCTCC 
ACCAGGATCC CCAAGATACA GAAGCTCCTG CAGGACTTCT TCAATGGAAG GGATCTCAAC 
AAAAGCATCA ACCCAGATGA GGCAGTGGCG TATGGAGCGG CGGTGCAGGC TGCCATCCTG 
ATGGGTGATA CCTCGGAAAA TGTCCAGGAC TTGTTGCTTC TGGATGTGGC TCCGTTGTCC 
CTGGGAATCG AGACGGCTGG AGGAGTCATG ACTGCACTCA TCAAACGTAA CACCACCATC 
CCCACCAAGC AGACGCAGAT CTTCTCCACA TATTCCGATA ACCAGCCGGG AGTCCTGATC 
CAGGTGTTTG AAGGAGAGAG GGCTATGACC AAAGATAACA ATTTGCTTGG CAAGTTTGAC 
CTTACTGGGA TCCCACCAGC ACCACGTGGT GTACCTCAAA TAGAGGTGAC CTTCGACATT 
GATGCCAATG GAATTCTTAA CGTATCCGCT GCCGACAAGA GCACGGGAAA AGAGAACAAA 
ATCACTATAA CCAATGACAA AGGTCGACTG AGCAAGGAGG ATATCGAGCG TATGGTGCAA 
GATGCGGAAA AGTACAAAGC CGAGGATGAA GTGCAGAGAG AGAAGATCGC AGCAAAGAAC 
GCTCTGGAGT CTTACGCCTT CAGTATGAAG AACACTGTTG AAGATGAGAA CCTGAGGGGA 
AAGATCAGCG AACAGGATAA GAAGAAGATC ATTGATAAGT GTACAGAGGT CGTTTCTTGG 
TTGGAGAACA ATCAGCTGGC TGAAAAGGAA GAGTATGAGC ATCATCAGAA GGAACTGGAA 
AGTGTGTGCA ATCCAATCAT CTCGCGGCTG TATCAGGGAG GAGCTCCTCC TGCAGGTGGA 
TGTGGATTCC AAGCCCAGCC TGAATCATCA CAGGGCCCCA CCATTGAAGA GGTGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093532.1
CDS	101..2020
Translation	MAKGLSIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNNTIFDAKRLIGRKFDDQVVQSDMKLWPFKVISEDGKPKVQVEYKGENKTFYPEEISSMVLVKMREIAEAYLGHRVNNAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDKGRKGERNVLIFDLGGGTFDVSILTIEDGIFEVKATAGDTHLGGEDFDNRMVKHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSSQASIEIDSLFEGIDFYTSITRARFEELNAELFRGTLDPVEKALRDAKMDKSQIQDIVLVGGSTRIPKIQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDTSENVQDLLLLDVAPLSLGIETAGGVMTALIKRNTTIPTKQTQIFSTYSDNQPGVLIQVFEGERAMTKDNNLLGKFDLTGIPPAPRGVPQIEVTFDIDANGILNVSAADKSTGKENKITITNDKGRLSKEDIERMVQDAEKYKAEDEVQREKIAAKNALESYAFSMKNTVEDENLRGKISEQDKKKIIDKCTEVVSWLENNQLAEKEEYEHHQKELESVCNPIISRLYQGGAPPAGGCGFQAQPESSQGPTIEEVD

Target ORF information:

RefSeq Version	NM_001100062.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio heat shock protein family A (Hsp70) member 1B (hspa1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100062.1

ORF Insert Sequence:

ATGGCTAAAG GTTTGTCTAT TGGCATTGAT CTGGGAACCA CTTACTCCTG TGTTGGAGTG 
TTCCAGCATG GCAAAGTGGA GATCATCGCT AACGATCAAG GAAACCGAAC CACTCCAAGC 
TATGTGGCTT TTACAGACAC AGAAAGACTC ATCGGAGATG CTGCCAAAAA CCAAGTCGCC 
ATGAACCCCA ACAACACCAT CTTTGACGCC AAACGACTGA TTGGCAGAAA GTTTGATGAT 
CAGGTTGTCC AGTCTGACAT GAAGCTTTGG CCATTTAAGG TCATCAGTGA AGATGGCAAA 
CCAAAAGTTC AAGTCGAGTA CAAAGGGGAA AACAAGACAT TCTACCCTGA GGAGATCTCA 
TCTATGGTCC TGGTCAAAAT GAGAGAGATT GCCGAAGCCT ACCTGGGTCA TAGAGTCAAT 
AATGCTGTCA TCACAGTCCC AGCGTACTTC AACGATTCAC AGAGGCAAGC GACCAAAGAC 
GCAGGGGTCA TTGCCGGATT GAACGTGCTG CGAATTATCA ATGAGCCCAC AGCAGCTGCC 
ATCGCCTACG GCCTGGACAA GGGCAGGAAG GGAGAGAGGA ATGTCCTGAT CTTCGACCTC 
GGCGGAGGCA CCTTTGATGT CTCCATCCTG ACGATTGAGG ATGGGATCTT TGAAGTGAAA 
GCCACAGCAG GGGACACTCA TCTGGGTGGA GAGGACTTCG ACAATCGCAT GGTGAAGCAC 
TTTGTTGAAG AGTTTAAGAG GAAGCACAAG AAGGACATCA GTCAGAATAA GAGAGCAGTA 
AGACGGCTGC GCACGGCCTG CGAGAGAGCT AAAAGGACCC TCTCATCTAG CTCTCAGGCT 
AGCATCGAGA TCGATTCCCT CTTTGAAGGC ATCGATTTCT ACACTTCCAT CACTAGGGCT 
CGCTTTGAAG AGCTTAACGC GGAGCTCTTT AGAGGCACGC TGGATCCAGT GGAAAAGGCT 
TTGAGAGATG CCAAAATGGA CAAGTCTCAG ATCCAAGATA TTGTTCTGGT TGGCGGCTCC 
ACCAGGATCC CCAAGATACA GAAGCTCCTG CAGGACTTCT TCAATGGAAG GGATCTCAAC 
AAAAGCATCA ACCCAGATGA GGCAGTGGCG TATGGAGCGG CGGTGCAGGC TGCCATCCTG 
ATGGGTGATA CCTCGGAAAA TGTCCAGGAC TTGTTGCTTC TGGATGTGGC TCCGTTGTCC 
CTGGGAATCG AGACGGCTGG AGGAGTCATG ACTGCACTCA TCAAACGTAA CACCACCATC 
CCCACCAAGC AGACGCAGAT CTTCTCCACA TATTCCGATA ACCAGCCGGG AGTCCTGATC 
CAGGTGTTTG AAGGAGAGAG GGCTATGACC AAAGATAACA ATTTGCTTGG CAAGTTTGAC 
CTTACTGGGA TCCCACCAGC ACCACGTGGT GTACCTCAAA TAGAGGTGAC CTTCGACATT 
GATGCCAATG GAATTCTTAA CGTATCCGCT GCCGACAAGA GCACGGGAAA AGAGAACAAA 
ATCACTATAA CCAATGACAA AGGTCGACTG AGCAAGGAGG ATATCGAGCG TATGGTGCAA 
GATGCGGAAA AGTACAAAGC CGAGGATGAA GTGCAGAGAG AGAAGATCGC AGCAAAGAAC 
GCTCTGGAGT CTTACGCCTT CAGTATGAAG AACACTGTTG AAGATGAGAA CCTGAGGGGA 
AAGATCAGCG AACAGGATAA GAAGAAGATC ATTGATAAGT GTACAGAGGT CGTTTCTTGG 
TTGGAGAACA ATCAGCTGGC TGAAAAGGAA GAGTATGAGC ATCATCAGAA GGAACTGGAA 
AGTGTGTGCA ATCCAATCAT CTCGCGGCTG TATCAGGGAG GAGCTCCTCC TGCAGGTGGA 
TGTGGATTCC AAGCCCAGCC TGAATCATCA CAGGGCCCCA CCATTGAAGA GGTGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hspa1b ( XM_021478327.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334002.1 Danio rerio si:ch211-199o1.2 (si:ch211-199o1.2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa31189
Clone ID Related Accession (Same CDS sequence)	XM_021478327.1 , NM_001100062.1
Accession Version	XM_021478327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC798846 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAAAG GTTTGTCTAT TGGCATTGAT CTGGGAACCA CTTACTCCTG TGTTGGAGTG 
TTCCAGCATG GCAAAGTGGA GATCATCGCT AACGATCAAG GAAACCGAAC CACTCCAAGC 
TATGTGGCTT TTACAGACAC AGAAAGACTC ATCGGAGATG CTGCCAAAAA CCAAGTCGCC 
ATGAACCCCA ACAACACCAT CTTTGACGCC AAACGACTGA TTGGCAGAAA GTTTGATGAT 
CAGGTTGTCC AGTCTGACAT GAAGCTTTGG CCATTTAAGG TCATCAGTGA AGATGGCAAA 
CCAAAAGTTC AAGTCGAGTA CAAAGGGGAA AACAAGACAT TCTACCCTGA GGAGATCTCA 
TCTATGGTCC TGGTCAAAAT GAGAGAGATT GCCGAAGCCT ACCTGGGTCA TAGAGTCAAT 
AATGCTGTCA TCACAGTCCC AGCGTACTTC AACGATTCAC AGAGGCAAGC GACCAAAGAC 
GCAGGGGTCA TTGCCGGATT GAACGTGCTG CGAATTATCA ATGAGCCCAC AGCAGCTGCC 
ATCGCCTACG GCCTGGACAA GGGCAGGAAG GGAGAGAGGA ATGTCCTGAT CTTCGACCTC 
GGCGGAGGCA CCTTTGATGT CTCCATCCTG ACGATTGAGG ATGGGATCTT TGAAGTGAAA 
GCCACAGCAG GGGACACTCA TCTGGGTGGA GAGGACTTCG ACAATCGCAT GGTGAAGCAC 
TTTGTTGAAG AGTTTAAGAG GAAGCACAAG AAGGACATCA GTCAGAATAA GAGAGCAGTA 
AGACGGCTGC GCACGGCCTG CGAGAGAGCT AAAAGGACCC TCTCATCTAG CTCTCAGGCT 
AGCATCGAGA TCGATTCCCT CTTTGAAGGC ATCGATTTCT ACACTTCCAT CACTAGGGCT 
CGCTTTGAAG AGCTTAACGC GGAGCTCTTT AGAGGCACGC TGGATCCAGT GGAAAAGGCT 
TTGAGAGATG CCAAAATGGA CAAGTCTCAG ATCCAAGATA TTGTTCTGGT TGGCGGCTCC 
ACCAGGATCC CCAAGATACA GAAGCTCCTG CAGGACTTCT TCAATGGAAG GGATCTCAAC 
AAAAGCATCA ACCCAGATGA GGCAGTGGCG TATGGAGCGG CGGTGCAGGC TGCCATCCTG 
ATGGGTGATA CCTCGGAAAA TGTCCAGGAC TTGTTGCTTC TGGATGTGGC TCCGTTGTCC 
CTGGGAATCG AGACGGCTGG AGGAGTCATG ACTGCACTCA TCAAACGTAA CACCACCATC 
CCCACCAAGC AGACGCAGAT CTTCTCCACA TATTCCGATA ACCAGCCGGG AGTCCTGATC 
CAGGTGTTTG AAGGAGAGAG GGCTATGACC AAAGATAACA ATTTGCTTGG CAAGTTTGAC 
CTTACTGGGA TCCCACCAGC ACCACGTGGT GTACCTCAAA TAGAGGTGAC CTTCGACATT 
GATGCCAATG GAATTCTTAA CGTATCCGCT GCCGACAAGA GCACGGGAAA AGAGAACAAA 
ATCACTATAA CCAATGACAA AGGTCGACTG AGCAAGGAGG ATATCGAGCG TATGGTGCAA 
GATGCGGAAA AGTACAAAGC CGAGGATGAA GTGCAGAGAG AGAAGATCGC AGCAAAGAAC 
GCTCTGGAGT CTTACGCCTT CAGTATGAAG AACACTGTTG AAGATGAGAA CCTGAGGGGA 
AAGATCAGCG AACAGGATAA GAAGAAGATC ATTGATAAGT GTACAGAGGT CGTTTCTTGG 
TTGGAGAACA ATCAGCTGGC TGAAAAGGAA GAGTATGAGC ATCATCAGAA GGAACTGGAA 
AGTGTGTGCA ATCCAATCAT CTCGCGGCTG TATCAGGGAG GAGCTCCTCC TGCAGGTGGA 
TGTGGATTCC AAGCCCAGCC TGAATCATCA CAGGGCCCCA CCATTGAAGA GGTGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334002.1
CDS	535..2454
Translation	MAKGLSIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNNTIFDAKRLIGRKFDDQVVQSDMKLWPFKVISEDGKPKVQVEYKGENKTFYPEEISSMVLVKMREIAEAYLGHRVNNAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDKGRKGERNVLIFDLGGGTFDVSILTIEDGIFEVKATAGDTHLGGEDFDNRMVKHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSSQASIEIDSLFEGIDFYTSITRARFEELNAELFRGTLDPVEKALRDAKMDKSQIQDIVLVGGSTRIPKIQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDTSENVQDLLLLDVAPLSLGIETAGGVMTALIKRNTTIPTKQTQIFSTYSDNQPGVLIQVFEGERAMTKDNNLLGKFDLTGIPPAPRGVPQIEVTFDIDANGILNVSAADKSTGKENKITITNDKGRLSKEDIERMVQDAEKYKAEDEVQREKIAAKNALESYAFSMKNTVEDENLRGKISEQDKKKIIDKCTEVVSWLENNQLAEKEEYEHHQKELESVCNPIISRLYQGGAPPAGGCGFQAQPESSQGPTIEEVD

Target ORF information:

RefSeq Version	XM_021478327.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-199o1.2 (si:ch211-199o1.2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478327.1

ORF Insert Sequence:

ATGGCTAAAG GTTTGTCTAT TGGCATTGAT CTGGGAACCA CTTACTCCTG TGTTGGAGTG 
TTCCAGCATG GCAAAGTGGA GATCATCGCT AACGATCAAG GAAACCGAAC CACTCCAAGC 
TATGTGGCTT TTACAGACAC AGAAAGACTC ATCGGAGATG CTGCCAAAAA CCAAGTCGCC 
ATGAACCCCA ACAACACCAT CTTTGACGCC AAACGACTGA TTGGCAGAAA GTTTGATGAT 
CAGGTTGTCC AGTCTGACAT GAAGCTTTGG CCATTTAAGG TCATCAGTGA AGATGGCAAA 
CCAAAAGTTC AAGTCGAGTA CAAAGGGGAA AACAAGACAT TCTACCCTGA GGAGATCTCA 
TCTATGGTCC TGGTCAAAAT GAGAGAGATT GCCGAAGCCT ACCTGGGTCA TAGAGTCAAT 
AATGCTGTCA TCACAGTCCC AGCGTACTTC AACGATTCAC AGAGGCAAGC GACCAAAGAC 
GCAGGGGTCA TTGCCGGATT GAACGTGCTG CGAATTATCA ATGAGCCCAC AGCAGCTGCC 
ATCGCCTACG GCCTGGACAA GGGCAGGAAG GGAGAGAGGA ATGTCCTGAT CTTCGACCTC 
GGCGGAGGCA CCTTTGATGT CTCCATCCTG ACGATTGAGG ATGGGATCTT TGAAGTGAAA 
GCCACAGCAG GGGACACTCA TCTGGGTGGA GAGGACTTCG ACAATCGCAT GGTGAAGCAC 
TTTGTTGAAG AGTTTAAGAG GAAGCACAAG AAGGACATCA GTCAGAATAA GAGAGCAGTA 
AGACGGCTGC GCACGGCCTG CGAGAGAGCT AAAAGGACCC TCTCATCTAG CTCTCAGGCT 
AGCATCGAGA TCGATTCCCT CTTTGAAGGC ATCGATTTCT ACACTTCCAT CACTAGGGCT 
CGCTTTGAAG AGCTTAACGC GGAGCTCTTT AGAGGCACGC TGGATCCAGT GGAAAAGGCT 
TTGAGAGATG CCAAAATGGA CAAGTCTCAG ATCCAAGATA TTGTTCTGGT TGGCGGCTCC 
ACCAGGATCC CCAAGATACA GAAGCTCCTG CAGGACTTCT TCAATGGAAG GGATCTCAAC 
AAAAGCATCA ACCCAGATGA GGCAGTGGCG TATGGAGCGG CGGTGCAGGC TGCCATCCTG 
ATGGGTGATA CCTCGGAAAA TGTCCAGGAC TTGTTGCTTC TGGATGTGGC TCCGTTGTCC 
CTGGGAATCG AGACGGCTGG AGGAGTCATG ACTGCACTCA TCAAACGTAA CACCACCATC 
CCCACCAAGC AGACGCAGAT CTTCTCCACA TATTCCGATA ACCAGCCGGG AGTCCTGATC 
CAGGTGTTTG AAGGAGAGAG GGCTATGACC AAAGATAACA ATTTGCTTGG CAAGTTTGAC 
CTTACTGGGA TCCCACCAGC ACCACGTGGT GTACCTCAAA TAGAGGTGAC CTTCGACATT 
GATGCCAATG GAATTCTTAA CGTATCCGCT GCCGACAAGA GCACGGGAAA AGAGAACAAA 
ATCACTATAA CCAATGACAA AGGTCGACTG AGCAAGGAGG ATATCGAGCG TATGGTGCAA 
GATGCGGAAA AGTACAAAGC CGAGGATGAA GTGCAGAGAG AGAAGATCGC AGCAAAGAAC 
GCTCTGGAGT CTTACGCCTT CAGTATGAAG AACACTGTTG AAGATGAGAA CCTGAGGGGA 
AAGATCAGCG AACAGGATAA GAAGAAGATC ATTGATAAGT GTACAGAGGT CGTTTCTTGG 
TTGGAGAACA ATCAGCTGGC TGAAAAGGAA GAGTATGAGC ATCATCAGAA GGAACTGGAA 
AGTGTGTGCA ATCCAATCAT CTCGCGGCTG TATCAGGGAG GAGCTCCTCC TGCAGGTGGA 
TGTGGATTCC AAGCCCAGCC TGAATCATCA CAGGGCCCCA CCATTGAAGA GGTGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The ATPase-dependent chaperoning activity of Hsp90a regulates thick filament formation and integration during skeletal muscle myofibrillogenesis.
Development (Cambridge, England)135(6)1147-56(2008 Mar)
Hawkins TA,Haramis AP,Etard C,Prodromou C,Vaughan CK,Ashworth R,Ray S,Behra M,Holder N,Talbot WS,Pearl LH,Str?hle U,Wilson SW

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hspa1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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