mkl2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mkl2a
Entrez Gene ID	793956
Full Name	MKL/myocardin-like 2a
Synonyms	si:ch211-86m22.3
General protein information	Preferred Names MKL/myocardin-like 2a Names MKL/myocardin-like 2a MKL/myocardin-like protein 2 zmp:0000000928
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009306704.2	XP_009304979.1	MKL/myocardin-like protein 2 isoform X2
XM_009306703.2	XP_009304978.1	MKL/myocardin-like protein 2 isoform X1
XM_009306704.3	XP_009304979.1	MKL/myocardin-like protein 2 isoform X2
XM_009306703.3	XP_009304978.1	MKL/myocardin-like protein 2 isoform X1
XM_021480299.1	XP_021335974.1	MKL/myocardin-like protein 2 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MKL2	XP_001146929.1
Gallus gallus (chicken)	MKL2	XP_004945127.1
Mus musculus (house mouse)	Mkl2	NP_001116139.1
Homo sapiens (human)	MKL2	NP_054767.3
	MKL2	XP_002802462.1
Canis lupus familiaris (dog)	MKL2	XP_005621582.1
Danio rerio (zebrafish)	mkl2a	XP_001333839.2
Danio rerio (zebrafish)	LOC100329890	XP_002663991.2
Bos taurus (cattle)	MKL2	NP_001179222.1
Xenopus tropicalis (tropical clawed frog)	mkl2	NP_001072650.1
Danio rerio (zebrafish)	mkl2b	XP_685503.5

Gene Ontology

The following mkl2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mkl2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa148337	XM_021480299.1 Latest version!	Danio rerio MKL/myocardin-like 2a (mkl2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa17828	XM_009306703.3 Latest version!	Danio rerio MKL/myocardin-like 2a (mkl2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa17829	XM_009306704.3 Latest version!	Danio rerio MKL/myocardin-like 2a (mkl2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
ODa17829	XM_009306704.2	Danio rerio MKL/myocardin-like 2a (mkl2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa17828	XM_009306703.2	Danio rerio MKL/myocardin-like 2a (mkl2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mkl2a ( XM_021480299.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335974.1 Danio rerio MKL/myocardin-like 2a (mkl2a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa148337
Clone ID Related Accession (Same CDS sequence)	XM_021480299.1
Accession Version	XM_021480299.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	MKL/myocardin-like protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAC AGGGGGATGC TGCGATTGGG GGACTGTTGG CACCCAGCCC ACAGAGTGAG 
GCTGTGACCC ATGAGATGGG TGAACTGTCC CTGCACCCCA GCCAGAGGCT TCCTCCTCTC 
AATGAGCGCA AAAATGTTCT CCAGCTGAGG CTCCAGCAGA GACGCACCCG GGAGCAGCTC 
GTTGAACAGG GCATCATGCC ACCCCTGAAG AGCCCTGCAG CTTTCCATGA ACAGATTCGC 
AGCCTTGAGA GAGCCAGGAC TGAAAATTTC CTAAAGCACA AAATACGCAG CCGCCCAGAA 
AGGGCTGAGT TGGTCCGCAT GCACATCTTA CAAGAGACCT TGGCAGAGCC TTCACTGCAG 
GCCACCCAGT TGAGACTAAA GAGAGCTCGA CTGGCTGATG ACCTCAATGA GAAGATTGCA 
CAGCGCCCCG GCCCAATGGA GCTGGTGGAA AAGAACATCC TGCCAGTGGA CTCCAGCCTC 
AAGGAGGCCA TAATTGATGG TCAGATGCAG TACCCTAGAA CTCTAGAAGA TCTAATAGAT 
GAAGACAGTG GAGATGCTTT TTCACCAGAG CAGCCAGCCA GTCAGGAGTC CCAGGGATCT 
GCAGCCTCAC CTGCAGAGGT CAGGACTATG GAGGAGACCT CACCGCTGCC CAATAACTTG 
CTACAGCACT TTCCTGCAGT CACCACTACT TCGACAGATT TCCTCAAGCC CTTTTCCACC 
GTTGAACAAT CCGTTAGCCC TCCAGCTCCC ATACCACAAC TAGTCACAGT AGCCCCTGTG 
AAGTCGGGGC CCACCCTTGT AAAGCAGAGT CAGCCCAAGT TACCTGGAGA CAAGAGCCGC 
AGCAAGAAGA GCAAGGAGTC CAAGCCACGT GTGAAAAAGT TGAAATATCA TCAGTACATT 
CCCCCAGATC AGAAACAAGA ACCCGACCAA GCCCCGATGG ACTCCTCGTA TGCTCGTTTA 
CTACAACAAC AGCAGCTATT TTTGCAGCTA CAGATCCTGA GCCAACAGCA ACAGCAGCAC 
TACAACTACC AGACCATCCT TCCTGCACCT CTCAAGCCTG TGATTGAAGG CCAAAACAGC 
AGTGCCACAG TGGCTATCAA CAATGCCAAT AGCCTTCCTG CCTCGATTGT AGTTTCTCTG 
CCCACAACAA CACCTGTGCG GCCCAATAGT ACTCTGTCCA ACCGCAAAGC TGGCATATTA 
CCAGCCAACC TGGAGGAGAT GAAGGTGGCA GAGCTGAAAA TGGAGTTGAA GTTGCGTGGT 
CTTCCAGTAT CAGGAACAAA AACTGACCTT ATTGAACGGC TCAAGCCTTT CCAAGAAAAT 
CCTATCACTA CTGCACCCAA CAACTCCTCC ACAGGACCTA AAACACAGCC CTGCGGTACA 
CCTATGGAAG TTTCCAGCAC CACTACAACC ATCTCCCCCA TCCAACTCCC CTCTAATACT 
CTGAGTTCCA CTCCACCAGT GTCTCCCAGT TCCTCAGAGC TTCTCAGCAG GGAGGACATG 
TCCGTGGAAG GACAAGCAGA GACTCAAAAC CGCTGTTTAA TTAAAGCAAA TGCTGTTCCT 
GAGGAACAAG ATCGGAGACT GCACGAAAAG GAGCGACAGA TTGAGGAATT GCTGCGTAAA 
TTGGAGCAGG AGCAGAAACT GGTGGAAGAA CTAAAGATGC AGCTAGAAGT GGAGAAACGA 
AGTGGAGTTG TTCCATCTGT GGAAAATAAC AACGTTTCTG GCCCAAGCAT CATGTTGGCA 
TCTGTCAAAG CAGAAAACAC TGTCCCTCCC AACTGCAAAC TGAATTCACA CACTACAACA 
ACTCTGGTTA AGCTTGAGGA GGCCCATCCC AACCAGGTGA CAACAGCCCC GCTCCGTCAG 
TTCATTATCA GCCATCAGGG GGTTCCACAA GTCATCGGGC AGCCCCAAAC ACTTCTAACC 
ACTCAACATG GGGGCACTCA GATCCTTCTT CCAATGCCCC TGGCGAATAA TACCACTACC 
ATCCAGCTGC CTAATGCTAA TGTCAAACTA CAGCCAGTCT TGCAAGCTGT TTCTACACCA 
GGCACAGGCC TAATTCAGAC GCCACAGCTG CAGTCTACCA AAGCAGAAAG TCCTACTTCA 
CAACAGCTTC TCAGTCACAA CCACATAGTT CAGACCCTGT CTGTGGGCAC CACTGAGAGG 
TCAGGTTTCC AGCACAGTAC CAGTGAGAAC CAGGCAGGCC TGGAGATTCC TCAGTGTTTC 
CTCAGTAGCT CCCCAGAGAA CAGAACCTCA CCTCAGACGT CCCCCATCCC ATCATCCCTT 
ATCAATGGAC AACTAAACAA GGCCTCTTTT ATCCAGCAGT GCCCAGTTTT CAACCAGGCT 
CCCAAGAACA GAGAGCCACC CCGTTATGAA GAGGCCGTCA AACAAACACG CAGCCTCCAG 
AATCCCACCA TCTCGGAGGA CGGGCAACTG TGGGCGACAT CTTTGAAGCG ATTCAAACAG 
AAGGGGACAA GGCAACATCC GGCCACACAC CTGTCCCAGA AACAAGAGAA TGACATGGCA 
CATGTTCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335974.1
CDS	285..2816
Translation	MEEQGDAAIGGLLAPSPQSEAVTHEMGELSLHPSQRLPPLNERKNVLQLRLQQRRTREQLVEQGIMPPLKSPAAFHEQIRSLERARTENFLKHKIRSRPERAELVRMHILQETLAEPSLQATQLRLKRARLADDLNEKIAQRPGPMELVEKNILPVDSSLKEAIIDGQMQYPRTLEDLIDEDSGDAFSPEQPASQESQGSAASPAEVRTMEETSPLPNNLLQHFPAVTTTSTDFLKPFSTVEQSVSPPAPIPQLVTVAPVKSGPTLVKQSQPKLPGDKSRSKKSKESKPRVKKLKYHQYIPPDQKQEPDQAPMDSSYARLLQQQQLFLQLQILSQQQQQHYNYQTILPAPLKPVIEGQNSSATVAINNANSLPASIVVSLPTTTPVRPNSTLSNRKAGILPANLEEMKVAELKMELKLRGLPVSGTKTDLIERLKPFQENPITTAPNNSSTGPKTQPCGTPMEVSSTTTTISPIQLPSNTLSSTPPVSPSSSELLSREDMSVEGQAETQNRCLIKANAVPEEQDRRLHEKERQIEELLRKLEQEQKLVEELKMQLEVEKRSGVVPSVENNNVSGPSIMLASVKAENTVPPNCKLNSHTTTTLVKLEEAHPNQVTTAPLRQFIISHQGVPQVIGQPQTLLTTQHGGTQILLPMPLANNTTTIQLPNANVKLQPVLQAVSTPGTGLIQTPQLQSTKAESPTSQQLLSHNHIVQTLSVGTTERSGFQHSTSENQAGLEIPQCFLSSSPENRTSPQTSPIPSSLINGQLNKASFIQQCPVFNQAPKNREPPRYEEAVKQTRSLQNPTISEDGQLWATSLKRFKQKGTRQHPATHLSQKQENDMAHVL

Target ORF information:

RefSeq Version	XM_021480299.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio MKL/myocardin-like 2a (mkl2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480299.1

ORF Insert Sequence:

ATGGAGGAAC AGGGGGATGC TGCGATTGGG GGACTGTTGG CACCCAGCCC ACAGAGTGAG 
GCTGTGACCC ATGAGATGGG TGAACTGTCC CTGCACCCCA GCCAGAGGCT TCCTCCTCTC 
AATGAGCGCA AAAATGTTCT CCAGCTGAGG CTCCAGCAGA GACGCACCCG GGAGCAGCTC 
GTTGAACAGG GCATCATGCC ACCCCTGAAG AGCCCTGCAG CTTTCCATGA ACAGATTCGC 
AGCCTTGAGA GAGCCAGGAC TGAAAATTTC CTAAAGCACA AAATACGCAG CCGCCCAGAA 
AGGGCTGAGT TGGTCCGCAT GCACATCTTA CAAGAGACCT TGGCAGAGCC TTCACTGCAG 
GCCACCCAGT TGAGACTAAA GAGAGCTCGA CTGGCTGATG ACCTCAATGA GAAGATTGCA 
CAGCGCCCCG GCCCAATGGA GCTGGTGGAA AAGAACATCC TGCCAGTGGA CTCCAGCCTC 
AAGGAGGCCA TAATTGATGG TCAGATGCAG TACCCTAGAA CTCTAGAAGA TCTAATAGAT 
GAAGACAGTG GAGATGCTTT TTCACCAGAG CAGCCAGCCA GTCAGGAGTC CCAGGGATCT 
GCAGCCTCAC CTGCAGAGGT CAGGACTATG GAGGAGACCT CACCGCTGCC CAATAACTTG 
CTACAGCACT TTCCTGCAGT CACCACTACT TCGACAGATT TCCTCAAGCC CTTTTCCACC 
GTTGAACAAT CCGTTAGCCC TCCAGCTCCC ATACCACAAC TAGTCACAGT AGCCCCTGTG 
AAGTCGGGGC CCACCCTTGT AAAGCAGAGT CAGCCCAAGT TACCTGGAGA CAAGAGCCGC 
AGCAAGAAGA GCAAGGAGTC CAAGCCACGT GTGAAAAAGT TGAAATATCA TCAGTACATT 
CCCCCAGATC AGAAACAAGA ACCCGACCAA GCCCCGATGG ACTCCTCGTA TGCTCGTTTA 
CTACAACAAC AGCAGCTATT TTTGCAGCTA CAGATCCTGA GCCAACAGCA ACAGCAGCAC 
TACAACTACC AGACCATCCT TCCTGCACCT CTCAAGCCTG TGATTGAAGG CCAAAACAGC 
AGTGCCACAG TGGCTATCAA CAATGCCAAT AGCCTTCCTG CCTCGATTGT AGTTTCTCTG 
CCCACAACAA CACCTGTGCG GCCCAATAGT ACTCTGTCCA ACCGCAAAGC TGGCATATTA 
CCAGCCAACC TGGAGGAGAT GAAGGTGGCA GAGCTGAAAA TGGAGTTGAA GTTGCGTGGT 
CTTCCAGTAT CAGGAACAAA AACTGACCTT ATTGAACGGC TCAAGCCTTT CCAAGAAAAT 
CCTATCACTA CTGCACCCAA CAACTCCTCC ACAGGACCTA AAACACAGCC CTGCGGTACA 
CCTATGGAAG TTTCCAGCAC CACTACAACC ATCTCCCCCA TCCAACTCCC CTCTAATACT 
CTGAGTTCCA CTCCACCAGT GTCTCCCAGT TCCTCAGAGC TTCTCAGCAG GGAGGACATG 
TCCGTGGAAG GACAAGCAGA GACTCAAAAC CGCTGTTTAA TTAAAGCAAA TGCTGTTCCT 
GAGGAACAAG ATCGGAGACT GCACGAAAAG GAGCGACAGA TTGAGGAATT GCTGCGTAAA 
TTGGAGCAGG AGCAGAAACT GGTGGAAGAA CTAAAGATGC AGCTAGAAGT GGAGAAACGA 
AGTGGAGTTG TTCCATCTGT GGAAAATAAC AACGTTTCTG GCCCAAGCAT CATGTTGGCA 
TCTGTCAAAG CAGAAAACAC TGTCCCTCCC AACTGCAAAC TGAATTCACA CACTACAACA 
ACTCTGGTTA AGCTTGAGGA GGCCCATCCC AACCAGGTGA CAACAGCCCC GCTCCGTCAG 
TTCATTATCA GCCATCAGGG GGTTCCACAA GTCATCGGGC AGCCCCAAAC ACTTCTAACC 
ACTCAACATG GGGGCACTCA GATCCTTCTT CCAATGCCCC TGGCGAATAA TACCACTACC 
ATCCAGCTGC CTAATGCTAA TGTCAAACTA CAGCCAGTCT TGCAAGCTGT TTCTACACCA 
GGCACAGGCC TAATTCAGAC GCCACAGCTG CAGTCTACCA AAGCAGAAAG TCCTACTTCA 
CAACAGCTTC TCAGTCACAA CCACATAGTT CAGACCCTGT CTGTGGGCAC CACTGAGAGG 
TCAGGTTTCC AGCACAGTAC CAGTGAGAAC CAGGCAGGCC TGGAGATTCC TCAGTGTTTC 
CTCAGTAGCT CCCCAGAGAA CAGAACCTCA CCTCAGACGT CCCCCATCCC ATCATCCCTT 
ATCAATGGAC AACTAAACAA GGCCTCTTTT ATCCAGCAGT GCCCAGTTTT CAACCAGGCT 
CCCAAGAACA GAGAGCCACC CCGTTATGAA GAGGCCGTCA AACAAACACG CAGCCTCCAG 
AATCCCACCA TCTCGGAGGA CGGGCAACTG TGGGCGACAT CTTTGAAGCG ATTCAAACAG 
AAGGGGACAA GGCAACATCC GGCCACACAC CTGTCCCAGA AACAAGAGAA TGACATGGCA 
CATGTTCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mkl2a ( XM_009306703.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304978.1 Danio rerio MKL/myocardin-like 2a (mkl2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17828
Clone ID Related Accession (Same CDS sequence)	XM_009306703.2 , XM_009306703.3
Accession Version	XM_009306703.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	MKL/myocardin-like protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306703.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAC AGGGGGATGC TGCGATTGGG GGACTGTTGG CACCCAGCCC ACAGAGTGAG 
GCTGTGACCC ATGAGATGGG TGAACTGTCC CTGCACCCCA GCCAGAGGCT TCCTCCTCTC 
AATGAGCGCA AAAATGTTCT CCAGCTGAGG CTCCAGCAGA GACGCACCCG GGAGCAGCTC 
GTTGAACAGG GCATCATGCC ACCCCTGAAG AGCCCTGCAG CTTTCCATGA ACAGATTCGC 
AGCCTTGAGA GAGCCAGGAC TGAAAATTTC CTAAAGCACA AAATACGCAG CCGCCCAGAA 
AGGGCTGAGT TGGTCCGCAT GCACATCTTA CAAGAGACCT TGGCAGAGCC TTCACTGCAG 
GCCACCCAGT TGAGACTAAA GAGAGCTCGA CTGGCTGATG ACCTCAATGA GAAGATTGCA 
CAGCGCCCCG GCCCAATGGA GCTGGTGGAA AAGAACATCC TGCCAGTGGA CTCCAGCCTC 
AAGGAGGCCA TAATTGATGG TCAGATGCAG TACCCTAGAA CTCTAGAAGA TCTAATAGAT 
GAAGACAGTG GAGATGCTTT TTCACCAGAG CAGCCAGCCA GTCAGGAGTC CCAGGGATCT 
GCAGCCTCAC CTGCAGAGGT CAGGACTATG GAGGAGACCT CACCGCTGCC CAATAACTTG 
CTACAGCACT TTCCTGCAGT CACCACTACT TCGACAGATT TCCTCAAGCC CTTTTCCACC 
GTTGAACAAT CCGTTAGCCC TCCAGCTCCC ATACCACAAC TAGTCACAGT AGCCCCTGTG 
AAGTCGGGGC CCACCCTTGT AAAGCAGAGT CAGCCCAAGT TACCTGGAGA CAAGAGCCGC 
AGCAAGAAGA GCAAGGAGTC CAAGCCACGT GTGAAAAAGT TGAAATATCA TCAGTACATT 
CCCCCAGATC AGAAACAAGA ACCCGACCAA GCCCCGATGG ACTCCTCGTA TGCTCGTTTA 
CTACAACAAC AGCAGCTATT TTTGCAGCTA CAGATCCTGA GCCAACAGCA ACAGCAGCAC 
TACAACTACC AGACCATCCT TCCTGCACCT CTCAAGCCTG TGATTGAAGG CCAAAACAGC 
AGTGCCACAG TGGCTATCAA CAATGCCAAT AGCCTTCCTG CCTCGATTGT AGTTTCTCTG 
CCCACAACAA CACCTGTGCG GCCCAATAGT ACTCTGTCCA ACCGCAAAGC TGGCATATTA 
CCAGCCAACC TGGAGGAGAT GAAGGTGGCA GAGCTGAAAA TGGAGTTGAA GTTGCGTGGT 
CTTCCAGTAT CAGGAACAAA AACTGACCTT ATTGAACGGC TCAAGCCTTT CCAAGAAAAT 
CCTATCACTA CTGCACCCAA CAACTCCTCC ACAGGACCTA AAACACAGCC CTGCGGTACA 
CCTATGGAAG TTTCCAGCAC CACTACAACC ATCTCCCCCA TCCAACTCCC CTCTAATACT 
CTGAGTTCCA CTCCACCAGT GTCTCCCAGT TCCTCAGAGC TTCTCAGCAG GGAGGACATG 
TCCGTGGAAG GACAAGCAGA GACTCAAAAC CGCTGTTTAA TTAAAGCAAA TGCTGTTCCT 
GAGGAACAAG ATCGGAGACT GCACGAAAAG GAGCGACAGA TTGAGGAATT GCTGCGTAAA 
TTGGAGCAGG AGCAGAAACT GGTGGAAGAA CTAAAGATGC AGCTAGAAGT GGAGAAACGA 
AGTGGAGTTG TTCCATCTGT GGAAAATAAC AACGTTTCTG GCCCAAGCAT CATGTTGGCA 
TCTGTCAAAG CAGAAAACAC TGTCCCTCCC AACTGCAAAC TGAATTCACA CACTACAACA 
ACTCTGGTTA AGCTTGAGGA GGCCCATCCC AACCAGGTGA CAACAGCCCC GCTCCGTCAG 
TTCATTATCA GCCATCAGGG GGTTCCACAA GTCATCGGGC AGCCCCAAAC ACTTCTAACC 
ACTCAACATG GGGGCACTCA GATCCTTCTT CCAATGCCCC TGGCGAATAA TACCACTACC 
ATCCAGCTGC CTAATGCTAA TGTCAAACTA CAGCCAGTCT TGCAAGCTGT TTCTACACCA 
GGCACAGGCC TAATTCAGAC GCCACAGCTG CAGTCTACCA AAGCAGAAAG TCCTACTTCA 
CAACAGCTTC TCAGTCACAA CCACATAGTT CAGACCCTGT CTGTGGGCAC CACTGAGAGG 
TCAGGTTTCC AGCACAGTAC CAGTGAGAAC CAGGCAGGCC TGGAGATTCC TCAGTGTTTC 
CTCAGTAGCT CCCCAGAGAA CAGAACCTCA CCTCAGACGT CCCCCATCCC ATCATCCCTT 
ATCAATGGAC AACTAAACAA GGCCTCTTTT ATCCAGCAGT GCCCAGTTTT CAACCAGGCT 
CCCAAGAACA GAGAGCCACC CCGTTATGAA GAGGCCGTCA AACAAACACG CAGCCTCCAG 
AATCCCACCA TCTCGGAGTT TCCCACAGTG ACGAGTCAAC AGATGGATGA TCTTTTTGAC 
ATTTTAATAG AAAGTGGAGA GATTACACCC TTTAGTCAAC AGGACCCATC TGTGCCTAAG 
ATGATGCCAG TCACGGCCAG CATCACCACT CTACCTATTA GCACAGCCCT GTCCCGACCG 
CCCGCACAGG TGCAGATGGC ACCCCCTCCA ACCCTCATGG TGGAGGCCTT GCCCAGCCTG 
GCCTCCCTGG ACTCCGACAA CCAATTAGAG GCTCTGCTGG AGGGCACATT AGTCGGGGAC 
ACAGAGCCAG AGCAGAGGAC GTTGTGCCTG CTAGAAGAGC TCCAAAACCA GATGCTGGAC 
CAACCAAACT CCCCAATGGA CACGTCCGAG CTTGGTTTCA GCGACCCGCC CACTCAGTCC 
TCATTCAGTC TGCAAGACAC CGGCCTGGAC AACATGGAGT GGCTGGACCT AACTATGCCA 
GGGCCCATTG GTGGACTCAA CCCACTGGGC ATCGCCTCTG AATTCCTGGA CACTCATGAC 
CTGCAGCTCC ACTGGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304978.1
CDS	422..3382
Translation	MEEQGDAAIGGLLAPSPQSEAVTHEMGELSLHPSQRLPPLNERKNVLQLRLQQRRTREQLVEQGIMPPLKSPAAFHEQIRSLERARTENFLKHKIRSRPERAELVRMHILQETLAEPSLQATQLRLKRARLADDLNEKIAQRPGPMELVEKNILPVDSSLKEAIIDGQMQYPRTLEDLIDEDSGDAFSPEQPASQESQGSAASPAEVRTMEETSPLPNNLLQHFPAVTTTSTDFLKPFSTVEQSVSPPAPIPQLVTVAPVKSGPTLVKQSQPKLPGDKSRSKKSKESKPRVKKLKYHQYIPPDQKQEPDQAPMDSSYARLLQQQQLFLQLQILSQQQQQHYNYQTILPAPLKPVIEGQNSSATVAINNANSLPASIVVSLPTTTPVRPNSTLSNRKAGILPANLEEMKVAELKMELKLRGLPVSGTKTDLIERLKPFQENPITTAPNNSSTGPKTQPCGTPMEVSSTTTTISPIQLPSNTLSSTPPVSPSSSELLSREDMSVEGQAETQNRCLIKANAVPEEQDRRLHEKERQIEELLRKLEQEQKLVEELKMQLEVEKRSGVVPSVENNNVSGPSIMLASVKAENTVPPNCKLNSHTTTTLVKLEEAHPNQVTTAPLRQFIISHQGVPQVIGQPQTLLTTQHGGTQILLPMPLANNTTTIQLPNANVKLQPVLQAVSTPGTGLIQTPQLQSTKAESPTSQQLLSHNHIVQTLSVGTTERSGFQHSTSENQAGLEIPQCFLSSSPENRTSPQTSPIPSSLINGQLNKASFIQQCPVFNQAPKNREPPRYEEAVKQTRSLQNPTISEFPTVTSQQMDDLFDILIESGEITPFSQQDPSVPKMMPVTASITTLPISTALSRPPAQVQMAPPPTLMVEALPSLASLDSDNQLEALLEGTLVGDTEPEQRTLCLLEELQNQMLDQPNSPMDTSELGFSDPPTQSSFSLQDTGLDNMEWLDLTMPGPIGGLNPLGIASEFLDTHDLQLHWD

Target ORF information:

RefSeq Version	XM_009306703.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio MKL/myocardin-like 2a (mkl2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306703.2

ORF Insert Sequence:

ATGGAGGAAC AGGGGGATGC TGCGATTGGG GGACTGTTGG CACCCAGCCC ACAGAGTGAG 
GCTGTGACCC ATGAGATGGG TGAACTGTCC CTGCACCCCA GCCAGAGGCT TCCTCCTCTC 
AATGAGCGCA AAAATGTTCT CCAGCTGAGG CTCCAGCAGA GACGCACCCG GGAGCAGCTC 
GTTGAACAGG GCATCATGCC ACCCCTGAAG AGCCCTGCAG CTTTCCATGA ACAGATTCGC 
AGCCTTGAGA GAGCCAGGAC TGAAAATTTC CTAAAGCACA AAATACGCAG CCGCCCAGAA 
AGGGCTGAGT TGGTCCGCAT GCACATCTTA CAAGAGACCT TGGCAGAGCC TTCACTGCAG 
GCCACCCAGT TGAGACTAAA GAGAGCTCGA CTGGCTGATG ACCTCAATGA GAAGATTGCA 
CAGCGCCCCG GCCCAATGGA GCTGGTGGAA AAGAACATCC TGCCAGTGGA CTCCAGCCTC 
AAGGAGGCCA TAATTGATGG TCAGATGCAG TACCCTAGAA CTCTAGAAGA TCTAATAGAT 
GAAGACAGTG GAGATGCTTT TTCACCAGAG CAGCCAGCCA GTCAGGAGTC CCAGGGATCT 
GCAGCCTCAC CTGCAGAGGT CAGGACTATG GAGGAGACCT CACCGCTGCC CAATAACTTG 
CTACAGCACT TTCCTGCAGT CACCACTACT TCGACAGATT TCCTCAAGCC CTTTTCCACC 
GTTGAACAAT CCGTTAGCCC TCCAGCTCCC ATACCACAAC TAGTCACAGT AGCCCCTGTG 
AAGTCGGGGC CCACCCTTGT AAAGCAGAGT CAGCCCAAGT TACCTGGAGA CAAGAGCCGC 
AGCAAGAAGA GCAAGGAGTC CAAGCCACGT GTGAAAAAGT TGAAATATCA TCAGTACATT 
CCCCCAGATC AGAAACAAGA ACCCGACCAA GCCCCGATGG ACTCCTCGTA TGCTCGTTTA 
CTACAACAAC AGCAGCTATT TTTGCAGCTA CAGATCCTGA GCCAACAGCA ACAGCAGCAC 
TACAACTACC AGACCATCCT TCCTGCACCT CTCAAGCCTG TGATTGAAGG CCAAAACAGC 
AGTGCCACAG TGGCTATCAA CAATGCCAAT AGCCTTCCTG CCTCGATTGT AGTTTCTCTG 
CCCACAACAA CACCTGTGCG GCCCAATAGT ACTCTGTCCA ACCGCAAAGC TGGCATATTA 
CCAGCCAACC TGGAGGAGAT GAAGGTGGCA GAGCTGAAAA TGGAGTTGAA GTTGCGTGGT 
CTTCCAGTAT CAGGAACAAA AACTGACCTT ATTGAACGGC TCAAGCCTTT CCAAGAAAAT 
CCTATCACTA CTGCACCCAA CAACTCCTCC ACAGGACCTA AAACACAGCC CTGCGGTACA 
CCTATGGAAG TTTCCAGCAC CACTACAACC ATCTCCCCCA TCCAACTCCC CTCTAATACT 
CTGAGTTCCA CTCCACCAGT GTCTCCCAGT TCCTCAGAGC TTCTCAGCAG GGAGGACATG 
TCCGTGGAAG GACAAGCAGA GACTCAAAAC CGCTGTTTAA TTAAAGCAAA TGCTGTTCCT 
GAGGAACAAG ATCGGAGACT GCACGAAAAG GAGCGACAGA TTGAGGAATT GCTGCGTAAA 
TTGGAGCAGG AGCAGAAACT GGTGGAAGAA CTAAAGATGC AGCTAGAAGT GGAGAAACGA 
AGTGGAGTTG TTCCATCTGT GGAAAATAAC AACGTTTCTG GCCCAAGCAT CATGTTGGCA 
TCTGTCAAAG CAGAAAACAC TGTCCCTCCC AACTGCAAAC TGAATTCACA CACTACAACA 
ACTCTGGTTA AGCTTGAGGA GGCCCATCCC AACCAGGTGA CAACAGCCCC GCTCCGTCAG 
TTCATTATCA GCCATCAGGG GGTTCCACAA GTCATCGGGC AGCCCCAAAC ACTTCTAACC 
ACTCAACATG GGGGCACTCA GATCCTTCTT CCAATGCCCC TGGCGAATAA TACCACTACC 
ATCCAGCTGC CTAATGCTAA TGTCAAACTA CAGCCAGTCT TGCAAGCTGT TTCTACACCA 
GGCACAGGCC TAATTCAGAC GCCACAGCTG CAGTCTACCA AAGCAGAAAG TCCTACTTCA 
CAACAGCTTC TCAGTCACAA CCACATAGTT CAGACCCTGT CTGTGGGCAC CACTGAGAGG 
TCAGGTTTCC AGCACAGTAC CAGTGAGAAC CAGGCAGGCC TGGAGATTCC TCAGTGTTTC 
CTCAGTAGCT CCCCAGAGAA CAGAACCTCA CCTCAGACGT CCCCCATCCC ATCATCCCTT 
ATCAATGGAC AACTAAACAA GGCCTCTTTT ATCCAGCAGT GCCCAGTTTT CAACCAGGCT 
CCCAAGAACA GAGAGCCACC CCGTTATGAA GAGGCCGTCA AACAAACACG CAGCCTCCAG 
AATCCCACCA TCTCGGAGTT TCCCACAGTG ACGAGTCAAC AGATGGATGA TCTTTTTGAC 
ATTTTAATAG AAAGTGGAGA GATTACACCC TTTAGTCAAC AGGACCCATC TGTGCCTAAG 
ATGATGCCAG TCACGGCCAG CATCACCACT CTACCTATTA GCACAGCCCT GTCCCGACCG 
CCCGCACAGG TGCAGATGGC ACCCCCTCCA ACCCTCATGG TGGAGGCCTT GCCCAGCCTG 
GCCTCCCTGG ACTCCGACAA CCAATTAGAG GCTCTGCTGG AGGGCACATT AGTCGGGGAC 
ACAGAGCCAG AGCAGAGGAC GTTGTGCCTG CTAGAAGAGC TCCAAAACCA GATGCTGGAC 
CAACCAAACT CCCCAATGGA CACGTCCGAG CTTGGTTTCA GCGACCCGCC CACTCAGTCC 
TCATTCAGTC TGCAAGACAC CGGCCTGGAC AACATGGAGT GGCTGGACCT AACTATGCCA 
GGGCCCATTG GTGGACTCAA CCCACTGGGC ATCGCCTCTG AATTCCTGGA CACTCATGAC 
CTGCAGCTCC ACTGGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mkl2a ( XM_009306703.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304978.1 Danio rerio MKL/myocardin-like 2a (mkl2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17828
Clone ID Related Accession (Same CDS sequence)	XM_009306703.2 , XM_009306703.3
Accession Version	XM_009306703.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	MKL/myocardin-like protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306703.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAC AGGGGGATGC TGCGATTGGG GGACTGTTGG CACCCAGCCC ACAGAGTGAG 
GCTGTGACCC ATGAGATGGG TGAACTGTCC CTGCACCCCA GCCAGAGGCT TCCTCCTCTC 
AATGAGCGCA AAAATGTTCT CCAGCTGAGG CTCCAGCAGA GACGCACCCG GGAGCAGCTC 
GTTGAACAGG GCATCATGCC ACCCCTGAAG AGCCCTGCAG CTTTCCATGA ACAGATTCGC 
AGCCTTGAGA GAGCCAGGAC TGAAAATTTC CTAAAGCACA AAATACGCAG CCGCCCAGAA 
AGGGCTGAGT TGGTCCGCAT GCACATCTTA CAAGAGACCT TGGCAGAGCC TTCACTGCAG 
GCCACCCAGT TGAGACTAAA GAGAGCTCGA CTGGCTGATG ACCTCAATGA GAAGATTGCA 
CAGCGCCCCG GCCCAATGGA GCTGGTGGAA AAGAACATCC TGCCAGTGGA CTCCAGCCTC 
AAGGAGGCCA TAATTGATGG TCAGATGCAG TACCCTAGAA CTCTAGAAGA TCTAATAGAT 
GAAGACAGTG GAGATGCTTT TTCACCAGAG CAGCCAGCCA GTCAGGAGTC CCAGGGATCT 
GCAGCCTCAC CTGCAGAGGT CAGGACTATG GAGGAGACCT CACCGCTGCC CAATAACTTG 
CTACAGCACT TTCCTGCAGT CACCACTACT TCGACAGATT TCCTCAAGCC CTTTTCCACC 
GTTGAACAAT CCGTTAGCCC TCCAGCTCCC ATACCACAAC TAGTCACAGT AGCCCCTGTG 
AAGTCGGGGC CCACCCTTGT AAAGCAGAGT CAGCCCAAGT TACCTGGAGA CAAGAGCCGC 
AGCAAGAAGA GCAAGGAGTC CAAGCCACGT GTGAAAAAGT TGAAATATCA TCAGTACATT 
CCCCCAGATC AGAAACAAGA ACCCGACCAA GCCCCGATGG ACTCCTCGTA TGCTCGTTTA 
CTACAACAAC AGCAGCTATT TTTGCAGCTA CAGATCCTGA GCCAACAGCA ACAGCAGCAC 
TACAACTACC AGACCATCCT TCCTGCACCT CTCAAGCCTG TGATTGAAGG CCAAAACAGC 
AGTGCCACAG TGGCTATCAA CAATGCCAAT AGCCTTCCTG CCTCGATTGT AGTTTCTCTG 
CCCACAACAA CACCTGTGCG GCCCAATAGT ACTCTGTCCA ACCGCAAAGC TGGCATATTA 
CCAGCCAACC TGGAGGAGAT GAAGGTGGCA GAGCTGAAAA TGGAGTTGAA GTTGCGTGGT 
CTTCCAGTAT CAGGAACAAA AACTGACCTT ATTGAACGGC TCAAGCCTTT CCAAGAAAAT 
CCTATCACTA CTGCACCCAA CAACTCCTCC ACAGGACCTA AAACACAGCC CTGCGGTACA 
CCTATGGAAG TTTCCAGCAC CACTACAACC ATCTCCCCCA TCCAACTCCC CTCTAATACT 
CTGAGTTCCA CTCCACCAGT GTCTCCCAGT TCCTCAGAGC TTCTCAGCAG GGAGGACATG 
TCCGTGGAAG GACAAGCAGA GACTCAAAAC CGCTGTTTAA TTAAAGCAAA TGCTGTTCCT 
GAGGAACAAG ATCGGAGACT GCACGAAAAG GAGCGACAGA TTGAGGAATT GCTGCGTAAA 
TTGGAGCAGG AGCAGAAACT GGTGGAAGAA CTAAAGATGC AGCTAGAAGT GGAGAAACGA 
AGTGGAGTTG TTCCATCTGT GGAAAATAAC AACGTTTCTG GCCCAAGCAT CATGTTGGCA 
TCTGTCAAAG CAGAAAACAC TGTCCCTCCC AACTGCAAAC TGAATTCACA CACTACAACA 
ACTCTGGTTA AGCTTGAGGA GGCCCATCCC AACCAGGTGA CAACAGCCCC GCTCCGTCAG 
TTCATTATCA GCCATCAGGG GGTTCCACAA GTCATCGGGC AGCCCCAAAC ACTTCTAACC 
ACTCAACATG GGGGCACTCA GATCCTTCTT CCAATGCCCC TGGCGAATAA TACCACTACC 
ATCCAGCTGC CTAATGCTAA TGTCAAACTA CAGCCAGTCT TGCAAGCTGT TTCTACACCA 
GGCACAGGCC TAATTCAGAC GCCACAGCTG CAGTCTACCA AAGCAGAAAG TCCTACTTCA 
CAACAGCTTC TCAGTCACAA CCACATAGTT CAGACCCTGT CTGTGGGCAC CACTGAGAGG 
TCAGGTTTCC AGCACAGTAC CAGTGAGAAC CAGGCAGGCC TGGAGATTCC TCAGTGTTTC 
CTCAGTAGCT CCCCAGAGAA CAGAACCTCA CCTCAGACGT CCCCCATCCC ATCATCCCTT 
ATCAATGGAC AACTAAACAA GGCCTCTTTT ATCCAGCAGT GCCCAGTTTT CAACCAGGCT 
CCCAAGAACA GAGAGCCACC CCGTTATGAA GAGGCCGTCA AACAAACACG CAGCCTCCAG 
AATCCCACCA TCTCGGAGTT TCCCACAGTG ACGAGTCAAC AGATGGATGA TCTTTTTGAC 
ATTTTAATAG AAAGTGGAGA GATTACACCC TTTAGTCAAC AGGACCCATC TGTGCCTAAG 
ATGATGCCAG TCACGGCCAG CATCACCACT CTACCTATTA GCACAGCCCT GTCCCGACCG 
CCCGCACAGG TGCAGATGGC ACCCCCTCCA ACCCTCATGG TGGAGGCCTT GCCCAGCCTG 
GCCTCCCTGG ACTCCGACAA CCAATTAGAG GCTCTGCTGG AGGGCACATT AGTCGGGGAC 
ACAGAGCCAG AGCAGAGGAC GTTGTGCCTG CTAGAAGAGC TCCAAAACCA GATGCTGGAC 
CAACCAAACT CCCCAATGGA CACGTCCGAG CTTGGTTTCA GCGACCCGCC CACTCAGTCC 
TCATTCAGTC TGCAAGACAC CGGCCTGGAC AACATGGAGT GGCTGGACCT AACTATGCCA 
GGGCCCATTG GTGGACTCAA CCCACTGGGC ATCGCCTCTG AATTCCTGGA CACTCATGAC 
CTGCAGCTCC ACTGGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304978.1
CDS	285..3245
Translation	MEEQGDAAIGGLLAPSPQSEAVTHEMGELSLHPSQRLPPLNERKNVLQLRLQQRRTREQLVEQGIMPPLKSPAAFHEQIRSLERARTENFLKHKIRSRPERAELVRMHILQETLAEPSLQATQLRLKRARLADDLNEKIAQRPGPMELVEKNILPVDSSLKEAIIDGQMQYPRTLEDLIDEDSGDAFSPEQPASQESQGSAASPAEVRTMEETSPLPNNLLQHFPAVTTTSTDFLKPFSTVEQSVSPPAPIPQLVTVAPVKSGPTLVKQSQPKLPGDKSRSKKSKESKPRVKKLKYHQYIPPDQKQEPDQAPMDSSYARLLQQQQLFLQLQILSQQQQQHYNYQTILPAPLKPVIEGQNSSATVAINNANSLPASIVVSLPTTTPVRPNSTLSNRKAGILPANLEEMKVAELKMELKLRGLPVSGTKTDLIERLKPFQENPITTAPNNSSTGPKTQPCGTPMEVSSTTTTISPIQLPSNTLSSTPPVSPSSSELLSREDMSVEGQAETQNRCLIKANAVPEEQDRRLHEKERQIEELLRKLEQEQKLVEELKMQLEVEKRSGVVPSVENNNVSGPSIMLASVKAENTVPPNCKLNSHTTTTLVKLEEAHPNQVTTAPLRQFIISHQGVPQVIGQPQTLLTTQHGGTQILLPMPLANNTTTIQLPNANVKLQPVLQAVSTPGTGLIQTPQLQSTKAESPTSQQLLSHNHIVQTLSVGTTERSGFQHSTSENQAGLEIPQCFLSSSPENRTSPQTSPIPSSLINGQLNKASFIQQCPVFNQAPKNREPPRYEEAVKQTRSLQNPTISEFPTVTSQQMDDLFDILIESGEITPFSQQDPSVPKMMPVTASITTLPISTALSRPPAQVQMAPPPTLMVEALPSLASLDSDNQLEALLEGTLVGDTEPEQRTLCLLEELQNQMLDQPNSPMDTSELGFSDPPTQSSFSLQDTGLDNMEWLDLTMPGPIGGLNPLGIASEFLDTHDLQLHWD

Target ORF information:

RefSeq Version	XM_009306703.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio MKL/myocardin-like 2a (mkl2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306703.3

ORF Insert Sequence:

ATGGAGGAAC AGGGGGATGC TGCGATTGGG GGACTGTTGG CACCCAGCCC ACAGAGTGAG 
GCTGTGACCC ATGAGATGGG TGAACTGTCC CTGCACCCCA GCCAGAGGCT TCCTCCTCTC 
AATGAGCGCA AAAATGTTCT CCAGCTGAGG CTCCAGCAGA GACGCACCCG GGAGCAGCTC 
GTTGAACAGG GCATCATGCC ACCCCTGAAG AGCCCTGCAG CTTTCCATGA ACAGATTCGC 
AGCCTTGAGA GAGCCAGGAC TGAAAATTTC CTAAAGCACA AAATACGCAG CCGCCCAGAA 
AGGGCTGAGT TGGTCCGCAT GCACATCTTA CAAGAGACCT TGGCAGAGCC TTCACTGCAG 
GCCACCCAGT TGAGACTAAA GAGAGCTCGA CTGGCTGATG ACCTCAATGA GAAGATTGCA 
CAGCGCCCCG GCCCAATGGA GCTGGTGGAA AAGAACATCC TGCCAGTGGA CTCCAGCCTC 
AAGGAGGCCA TAATTGATGG TCAGATGCAG TACCCTAGAA CTCTAGAAGA TCTAATAGAT 
GAAGACAGTG GAGATGCTTT TTCACCAGAG CAGCCAGCCA GTCAGGAGTC CCAGGGATCT 
GCAGCCTCAC CTGCAGAGGT CAGGACTATG GAGGAGACCT CACCGCTGCC CAATAACTTG 
CTACAGCACT TTCCTGCAGT CACCACTACT TCGACAGATT TCCTCAAGCC CTTTTCCACC 
GTTGAACAAT CCGTTAGCCC TCCAGCTCCC ATACCACAAC TAGTCACAGT AGCCCCTGTG 
AAGTCGGGGC CCACCCTTGT AAAGCAGAGT CAGCCCAAGT TACCTGGAGA CAAGAGCCGC 
AGCAAGAAGA GCAAGGAGTC CAAGCCACGT GTGAAAAAGT TGAAATATCA TCAGTACATT 
CCCCCAGATC AGAAACAAGA ACCCGACCAA GCCCCGATGG ACTCCTCGTA TGCTCGTTTA 
CTACAACAAC AGCAGCTATT TTTGCAGCTA CAGATCCTGA GCCAACAGCA ACAGCAGCAC 
TACAACTACC AGACCATCCT TCCTGCACCT CTCAAGCCTG TGATTGAAGG CCAAAACAGC 
AGTGCCACAG TGGCTATCAA CAATGCCAAT AGCCTTCCTG CCTCGATTGT AGTTTCTCTG 
CCCACAACAA CACCTGTGCG GCCCAATAGT ACTCTGTCCA ACCGCAAAGC TGGCATATTA 
CCAGCCAACC TGGAGGAGAT GAAGGTGGCA GAGCTGAAAA TGGAGTTGAA GTTGCGTGGT 
CTTCCAGTAT CAGGAACAAA AACTGACCTT ATTGAACGGC TCAAGCCTTT CCAAGAAAAT 
CCTATCACTA CTGCACCCAA CAACTCCTCC ACAGGACCTA AAACACAGCC CTGCGGTACA 
CCTATGGAAG TTTCCAGCAC CACTACAACC ATCTCCCCCA TCCAACTCCC CTCTAATACT 
CTGAGTTCCA CTCCACCAGT GTCTCCCAGT TCCTCAGAGC TTCTCAGCAG GGAGGACATG 
TCCGTGGAAG GACAAGCAGA GACTCAAAAC CGCTGTTTAA TTAAAGCAAA TGCTGTTCCT 
GAGGAACAAG ATCGGAGACT GCACGAAAAG GAGCGACAGA TTGAGGAATT GCTGCGTAAA 
TTGGAGCAGG AGCAGAAACT GGTGGAAGAA CTAAAGATGC AGCTAGAAGT GGAGAAACGA 
AGTGGAGTTG TTCCATCTGT GGAAAATAAC AACGTTTCTG GCCCAAGCAT CATGTTGGCA 
TCTGTCAAAG CAGAAAACAC TGTCCCTCCC AACTGCAAAC TGAATTCACA CACTACAACA 
ACTCTGGTTA AGCTTGAGGA GGCCCATCCC AACCAGGTGA CAACAGCCCC GCTCCGTCAG 
TTCATTATCA GCCATCAGGG GGTTCCACAA GTCATCGGGC AGCCCCAAAC ACTTCTAACC 
ACTCAACATG GGGGCACTCA GATCCTTCTT CCAATGCCCC TGGCGAATAA TACCACTACC 
ATCCAGCTGC CTAATGCTAA TGTCAAACTA CAGCCAGTCT TGCAAGCTGT TTCTACACCA 
GGCACAGGCC TAATTCAGAC GCCACAGCTG CAGTCTACCA AAGCAGAAAG TCCTACTTCA 
CAACAGCTTC TCAGTCACAA CCACATAGTT CAGACCCTGT CTGTGGGCAC CACTGAGAGG 
TCAGGTTTCC AGCACAGTAC CAGTGAGAAC CAGGCAGGCC TGGAGATTCC TCAGTGTTTC 
CTCAGTAGCT CCCCAGAGAA CAGAACCTCA CCTCAGACGT CCCCCATCCC ATCATCCCTT 
ATCAATGGAC AACTAAACAA GGCCTCTTTT ATCCAGCAGT GCCCAGTTTT CAACCAGGCT 
CCCAAGAACA GAGAGCCACC CCGTTATGAA GAGGCCGTCA AACAAACACG CAGCCTCCAG 
AATCCCACCA TCTCGGAGTT TCCCACAGTG ACGAGTCAAC AGATGGATGA TCTTTTTGAC 
ATTTTAATAG AAAGTGGAGA GATTACACCC TTTAGTCAAC AGGACCCATC TGTGCCTAAG 
ATGATGCCAG TCACGGCCAG CATCACCACT CTACCTATTA GCACAGCCCT GTCCCGACCG 
CCCGCACAGG TGCAGATGGC ACCCCCTCCA ACCCTCATGG TGGAGGCCTT GCCCAGCCTG 
GCCTCCCTGG ACTCCGACAA CCAATTAGAG GCTCTGCTGG AGGGCACATT AGTCGGGGAC 
ACAGAGCCAG AGCAGAGGAC GTTGTGCCTG CTAGAAGAGC TCCAAAACCA GATGCTGGAC 
CAACCAAACT CCCCAATGGA CACGTCCGAG CTTGGTTTCA GCGACCCGCC CACTCAGTCC 
TCATTCAGTC TGCAAGACAC CGGCCTGGAC AACATGGAGT GGCTGGACCT AACTATGCCA 
GGGCCCATTG GTGGACTCAA CCCACTGGGC ATCGCCTCTG AATTCCTGGA CACTCATGAC 
CTGCAGCTCC ACTGGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mkl2a ( XM_009306704.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304979.1 Danio rerio MKL/myocardin-like 2a (mkl2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa17829
Clone ID Related Accession (Same CDS sequence)	XM_009306704.2 , XM_009306704.3
Accession Version	XM_009306704.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	MKL/myocardin-like protein 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306704.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAC AGGGGGATGC TGCGATTGGG GGACTGTTGG CACCCAGCCC ACAGAGTGAG 
GCTGTGACCC ATGAGATGGG TGAACTGTCC CTGCACCCCA GCCAGAGGCT TCCTCCTCTC 
AATGAGCGCA AAAATGTTCT CCAGCTGAGG CTCCAGCAGA GACGCACCCG GGAGCAGCTC 
GTTGAACAGG GCATCATGCC ACCCCTGAAG AGCCCTGCAG CTTTCCATGA ACAGATTCGC 
AGCCTTGAGA GAGCCAGGAC TGAAAATTTC CTAAAGCACA AAATACGCAG CCGCCCAGAA 
AGGGCTGAGT TGGTCCGCAT GCACATCTTA CAAGAGACCT TGGCAGAGCC TTCACTGCAG 
GCCACCCAGT TGAGACTAAA GAGAGCTCGA CTGGCTGATG ACCTCAATGA GAAGATTGCA 
CAGCGCCCCG GCCCAATGGA GCTGGTGGAA AAGAACATCC TGCCAGTGGA CTCCAGCCTC 
AAGGAGGCCA TAATTGATGG TCAGATGCAG TACCCTAGAA CTCTAGAAGA TCTAATAGAT 
GAAGACAGTG GAGATGCTTT TTCACCAGAG CAGCCAGCCA GTCAGGAGTC CCAGGGATCT 
GCAGCCTCAC CTGCAGAGGT CAGGACTATG GAGGAGACCT CACCGCTGCC CAATAACTTG 
CTACAGCACT TTCCTGCAGT CACCACTACT TCGACAGATT TCCTCAAGCC CTTTTCCACC 
GTTGAACAAT CCGTTAGCCC TCCAGCTCCC ATACCACAAC TAGTCACAGT AGCCCCTGTG 
AAGTCGGGGC CCACCCTTGT AAAGAGTCAG CCCAAGTTAC CTGGAGACAA GAGCCGCAGC 
AAGAAGAGCA AGGAGTCCAA GCCACGTGTG AAAAAGTTGA AATATCATCA GTACATTCCC 
CCAGATCAGA AACAAGAACC CGACCAAGCC CCGATGGACT CCTCGTATGC TCGTTTACTA 
CAACAACAGC AGCTATTTTT GCAGCTACAG ATCCTGAGCC AACAGCAACA GCAGCACTAC 
AACTACCAGA CCATCCTTCC TGCACCTCTC AAGCCTGTGA TTGAAGGCCA AAACAGCAGT 
GCCACAGTGG CTATCAACAA TGCCAATAGC CTTCCTGCCT CGATTGTAGT TTCTCTGCCC 
ACAACAACAC CTGTGCGGCC CAATAGTACT CTGTCCAACC GCAAAGCTGG CATATTACCA 
GCCAACCTGG AGGAGATGAA GGTGGCAGAG CTGAAAATGG AGTTGAAGTT GCGTGGTCTT 
CCAGTATCAG GAACAAAAAC TGACCTTATT GAACGGCTCA AGCCTTTCCA AGAAAATCCT 
ATCACTACTG CACCCAACAA CTCCTCCACA GGACCTAAAA CACAGCCCTG CGGTACACCT 
ATGGAAGTTT CCAGCACCAC TACAACCATC TCCCCCATCC AACTCCCCTC TAATACTCTG 
AGTTCCACTC CACCAGTGTC TCCCAGTTCC TCAGAGCTTC TCAGCAGGGA GGACATGTCC 
GTGGAAGGAC AAGCAGAGAC TCAAAACCGC TGTTTAATTA AAGCAAATGC TGTTCCTGAG 
GAACAAGATC GGAGACTGCA CGAAAAGGAG CGACAGATTG AGGAATTGCT GCGTAAATTG 
GAGCAGGAGC AGAAACTGGT GGAAGAACTA AAGATGCAGC TAGAAGTGGA GAAACGAAGT 
GGAGTTGTTC CATCTGTGGA AAATAACAAC GTTTCTGGCC CAAGCATCAT GTTGGCATCT 
GTCAAAGCAG AAAACACTGT CCCTCCCAAC TGCAAACTGA ATTCACACAC TACAACAACT 
CTGGTTAAGC TTGAGGAGGC CCATCCCAAC CAGGTGACAA CAGCCCCGCT CCGTCAGTTC 
ATTATCAGCC ATCAGGGGGT TCCACAAGTC ATCGGGCAGC CCCAAACACT TCTAACCACT 
CAACATGGGG GCACTCAGAT CCTTCTTCCA ATGCCCCTGG CGAATAATAC CACTACCATC 
CAGCTGCCTA ATGCTAATGT CAAACTACAG CCAGTCTTGC AAGCTGTTTC TACACCAGGC 
ACAGGCCTAA TTCAGACGCC ACAGCTGCAG TCTACCAAAG CAGAAAGTCC TACTTCACAA 
CAGCTTCTCA GTCACAACCA CATAGTTCAG ACCCTGTCTG TGGGCACCAC TGAGAGGTCA 
GGTTTCCAGC ACAGTACCAG TGAGAACCAG GCAGGCCTGG AGATTCCTCA GTGTTTCCTC 
AGTAGCTCCC CAGAGAACAG AACCTCACCT CAGACGTCCC CCATCCCATC ATCCCTTATC 
AATGGACAAC TAAACAAGGC CTCTTTTATC CAGCAGTGCC CAGTTTTCAA CCAGGCTCCC 
AAGAACAGAG AGCCACCCCG TTATGAAGAG GCCGTCAAAC AAACACGCAG CCTCCAGAAT 
CCCACCATCT CGGAGTTTCC CACAGTGACG AGTCAACAGA TGGATGATCT TTTTGACATT 
TTAATAGAAA GTGGAGAGAT TACACCCTTT AGTCAACAGG ACCCATCTGT GCCTAAGATG 
ATGCCAGTCA CGGCCAGCAT CACCACTCTA CCTATTAGCA CAGCCCTGTC CCGACCGCCC 
GCACAGGTGC AGATGGCACC CCCTCCAACC CTCATGGTGG AGGCCTTGCC CAGCCTGGCC 
TCCCTGGACT CCGACAACCA ATTAGAGGCT CTGCTGGAGG GCACATTAGT CGGGGACACA 
GAGCCAGAGC AGAGGACGTT GTGCCTGCTA GAAGAGCTCC AAAACCAGAT GCTGGACCAA 
CCAAACTCCC CAATGGACAC GTCCGAGCTT GGTTTCAGCG ACCCGCCCAC TCAGTCCTCA 
TTCAGTCTGC AAGACACCGG CCTGGACAAC ATGGAGTGGC TGGACCTAAC TATGCCAGGG 
CCCATTGGTG GACTCAACCC ACTGGGCATC GCCTCTGAAT TCCTGGACAC TCATGACCTG 
CAGCTCCACT GGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304979.1
CDS	422..3379
Translation	MEEQGDAAIGGLLAPSPQSEAVTHEMGELSLHPSQRLPPLNERKNVLQLRLQQRRTREQLVEQGIMPPLKSPAAFHEQIRSLERARTENFLKHKIRSRPERAELVRMHILQETLAEPSLQATQLRLKRARLADDLNEKIAQRPGPMELVEKNILPVDSSLKEAIIDGQMQYPRTLEDLIDEDSGDAFSPEQPASQESQGSAASPAEVRTMEETSPLPNNLLQHFPAVTTTSTDFLKPFSTVEQSVSPPAPIPQLVTVAPVKSGPTLVKSQPKLPGDKSRSKKSKESKPRVKKLKYHQYIPPDQKQEPDQAPMDSSYARLLQQQQLFLQLQILSQQQQQHYNYQTILPAPLKPVIEGQNSSATVAINNANSLPASIVVSLPTTTPVRPNSTLSNRKAGILPANLEEMKVAELKMELKLRGLPVSGTKTDLIERLKPFQENPITTAPNNSSTGPKTQPCGTPMEVSSTTTTISPIQLPSNTLSSTPPVSPSSSELLSREDMSVEGQAETQNRCLIKANAVPEEQDRRLHEKERQIEELLRKLEQEQKLVEELKMQLEVEKRSGVVPSVENNNVSGPSIMLASVKAENTVPPNCKLNSHTTTTLVKLEEAHPNQVTTAPLRQFIISHQGVPQVIGQPQTLLTTQHGGTQILLPMPLANNTTTIQLPNANVKLQPVLQAVSTPGTGLIQTPQLQSTKAESPTSQQLLSHNHIVQTLSVGTTERSGFQHSTSENQAGLEIPQCFLSSSPENRTSPQTSPIPSSLINGQLNKASFIQQCPVFNQAPKNREPPRYEEAVKQTRSLQNPTISEFPTVTSQQMDDLFDILIESGEITPFSQQDPSVPKMMPVTASITTLPISTALSRPPAQVQMAPPPTLMVEALPSLASLDSDNQLEALLEGTLVGDTEPEQRTLCLLEELQNQMLDQPNSPMDTSELGFSDPPTQSSFSLQDTGLDNMEWLDLTMPGPIGGLNPLGIASEFLDTHDLQLHWD

Target ORF information:

RefSeq Version	XM_009306704.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio MKL/myocardin-like 2a (mkl2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306704.2

ORF Insert Sequence:

ATGGAGGAAC AGGGGGATGC TGCGATTGGG GGACTGTTGG CACCCAGCCC ACAGAGTGAG 
GCTGTGACCC ATGAGATGGG TGAACTGTCC CTGCACCCCA GCCAGAGGCT TCCTCCTCTC 
AATGAGCGCA AAAATGTTCT CCAGCTGAGG CTCCAGCAGA GACGCACCCG GGAGCAGCTC 
GTTGAACAGG GCATCATGCC ACCCCTGAAG AGCCCTGCAG CTTTCCATGA ACAGATTCGC 
AGCCTTGAGA GAGCCAGGAC TGAAAATTTC CTAAAGCACA AAATACGCAG CCGCCCAGAA 
AGGGCTGAGT TGGTCCGCAT GCACATCTTA CAAGAGACCT TGGCAGAGCC TTCACTGCAG 
GCCACCCAGT TGAGACTAAA GAGAGCTCGA CTGGCTGATG ACCTCAATGA GAAGATTGCA 
CAGCGCCCCG GCCCAATGGA GCTGGTGGAA AAGAACATCC TGCCAGTGGA CTCCAGCCTC 
AAGGAGGCCA TAATTGATGG TCAGATGCAG TACCCTAGAA CTCTAGAAGA TCTAATAGAT 
GAAGACAGTG GAGATGCTTT TTCACCAGAG CAGCCAGCCA GTCAGGAGTC CCAGGGATCT 
GCAGCCTCAC CTGCAGAGGT CAGGACTATG GAGGAGACCT CACCGCTGCC CAATAACTTG 
CTACAGCACT TTCCTGCAGT CACCACTACT TCGACAGATT TCCTCAAGCC CTTTTCCACC 
GTTGAACAAT CCGTTAGCCC TCCAGCTCCC ATACCACAAC TAGTCACAGT AGCCCCTGTG 
AAGTCGGGGC CCACCCTTGT AAAGAGTCAG CCCAAGTTAC CTGGAGACAA GAGCCGCAGC 
AAGAAGAGCA AGGAGTCCAA GCCACGTGTG AAAAAGTTGA AATATCATCA GTACATTCCC 
CCAGATCAGA AACAAGAACC CGACCAAGCC CCGATGGACT CCTCGTATGC TCGTTTACTA 
CAACAACAGC AGCTATTTTT GCAGCTACAG ATCCTGAGCC AACAGCAACA GCAGCACTAC 
AACTACCAGA CCATCCTTCC TGCACCTCTC AAGCCTGTGA TTGAAGGCCA AAACAGCAGT 
GCCACAGTGG CTATCAACAA TGCCAATAGC CTTCCTGCCT CGATTGTAGT TTCTCTGCCC 
ACAACAACAC CTGTGCGGCC CAATAGTACT CTGTCCAACC GCAAAGCTGG CATATTACCA 
GCCAACCTGG AGGAGATGAA GGTGGCAGAG CTGAAAATGG AGTTGAAGTT GCGTGGTCTT 
CCAGTATCAG GAACAAAAAC TGACCTTATT GAACGGCTCA AGCCTTTCCA AGAAAATCCT 
ATCACTACTG CACCCAACAA CTCCTCCACA GGACCTAAAA CACAGCCCTG CGGTACACCT 
ATGGAAGTTT CCAGCACCAC TACAACCATC TCCCCCATCC AACTCCCCTC TAATACTCTG 
AGTTCCACTC CACCAGTGTC TCCCAGTTCC TCAGAGCTTC TCAGCAGGGA GGACATGTCC 
GTGGAAGGAC AAGCAGAGAC TCAAAACCGC TGTTTAATTA AAGCAAATGC TGTTCCTGAG 
GAACAAGATC GGAGACTGCA CGAAAAGGAG CGACAGATTG AGGAATTGCT GCGTAAATTG 
GAGCAGGAGC AGAAACTGGT GGAAGAACTA AAGATGCAGC TAGAAGTGGA GAAACGAAGT 
GGAGTTGTTC CATCTGTGGA AAATAACAAC GTTTCTGGCC CAAGCATCAT GTTGGCATCT 
GTCAAAGCAG AAAACACTGT CCCTCCCAAC TGCAAACTGA ATTCACACAC TACAACAACT 
CTGGTTAAGC TTGAGGAGGC CCATCCCAAC CAGGTGACAA CAGCCCCGCT CCGTCAGTTC 
ATTATCAGCC ATCAGGGGGT TCCACAAGTC ATCGGGCAGC CCCAAACACT TCTAACCACT 
CAACATGGGG GCACTCAGAT CCTTCTTCCA ATGCCCCTGG CGAATAATAC CACTACCATC 
CAGCTGCCTA ATGCTAATGT CAAACTACAG CCAGTCTTGC AAGCTGTTTC TACACCAGGC 
ACAGGCCTAA TTCAGACGCC ACAGCTGCAG TCTACCAAAG CAGAAAGTCC TACTTCACAA 
CAGCTTCTCA GTCACAACCA CATAGTTCAG ACCCTGTCTG TGGGCACCAC TGAGAGGTCA 
GGTTTCCAGC ACAGTACCAG TGAGAACCAG GCAGGCCTGG AGATTCCTCA GTGTTTCCTC 
AGTAGCTCCC CAGAGAACAG AACCTCACCT CAGACGTCCC CCATCCCATC ATCCCTTATC 
AATGGACAAC TAAACAAGGC CTCTTTTATC CAGCAGTGCC CAGTTTTCAA CCAGGCTCCC 
AAGAACAGAG AGCCACCCCG TTATGAAGAG GCCGTCAAAC AAACACGCAG CCTCCAGAAT 
CCCACCATCT CGGAGTTTCC CACAGTGACG AGTCAACAGA TGGATGATCT TTTTGACATT 
TTAATAGAAA GTGGAGAGAT TACACCCTTT AGTCAACAGG ACCCATCTGT GCCTAAGATG 
ATGCCAGTCA CGGCCAGCAT CACCACTCTA CCTATTAGCA CAGCCCTGTC CCGACCGCCC 
GCACAGGTGC AGATGGCACC CCCTCCAACC CTCATGGTGG AGGCCTTGCC CAGCCTGGCC 
TCCCTGGACT CCGACAACCA ATTAGAGGCT CTGCTGGAGG GCACATTAGT CGGGGACACA 
GAGCCAGAGC AGAGGACGTT GTGCCTGCTA GAAGAGCTCC AAAACCAGAT GCTGGACCAA 
CCAAACTCCC CAATGGACAC GTCCGAGCTT GGTTTCAGCG ACCCGCCCAC TCAGTCCTCA 
TTCAGTCTGC AAGACACCGG CCTGGACAAC ATGGAGTGGC TGGACCTAAC TATGCCAGGG 
CCCATTGGTG GACTCAACCC ACTGGGCATC GCCTCTGAAT TCCTGGACAC TCATGACCTG 
CAGCTCCACT GGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mkl2a ( XM_009306704.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304979.1 Danio rerio MKL/myocardin-like 2a (mkl2a), transcript variant X2, mRNA.

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CloneID	ODa17829
Clone ID Related Accession (Same CDS sequence)	XM_009306704.2 , XM_009306704.3
Accession Version	XM_009306704.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	MKL/myocardin-like protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306704.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAC AGGGGGATGC TGCGATTGGG GGACTGTTGG CACCCAGCCC ACAGAGTGAG 
GCTGTGACCC ATGAGATGGG TGAACTGTCC CTGCACCCCA GCCAGAGGCT TCCTCCTCTC 
AATGAGCGCA AAAATGTTCT CCAGCTGAGG CTCCAGCAGA GACGCACCCG GGAGCAGCTC 
GTTGAACAGG GCATCATGCC ACCCCTGAAG AGCCCTGCAG CTTTCCATGA ACAGATTCGC 
AGCCTTGAGA GAGCCAGGAC TGAAAATTTC CTAAAGCACA AAATACGCAG CCGCCCAGAA 
AGGGCTGAGT TGGTCCGCAT GCACATCTTA CAAGAGACCT TGGCAGAGCC TTCACTGCAG 
GCCACCCAGT TGAGACTAAA GAGAGCTCGA CTGGCTGATG ACCTCAATGA GAAGATTGCA 
CAGCGCCCCG GCCCAATGGA GCTGGTGGAA AAGAACATCC TGCCAGTGGA CTCCAGCCTC 
AAGGAGGCCA TAATTGATGG TCAGATGCAG TACCCTAGAA CTCTAGAAGA TCTAATAGAT 
GAAGACAGTG GAGATGCTTT TTCACCAGAG CAGCCAGCCA GTCAGGAGTC CCAGGGATCT 
GCAGCCTCAC CTGCAGAGGT CAGGACTATG GAGGAGACCT CACCGCTGCC CAATAACTTG 
CTACAGCACT TTCCTGCAGT CACCACTACT TCGACAGATT TCCTCAAGCC CTTTTCCACC 
GTTGAACAAT CCGTTAGCCC TCCAGCTCCC ATACCACAAC TAGTCACAGT AGCCCCTGTG 
AAGTCGGGGC CCACCCTTGT AAAGAGTCAG CCCAAGTTAC CTGGAGACAA GAGCCGCAGC 
AAGAAGAGCA AGGAGTCCAA GCCACGTGTG AAAAAGTTGA AATATCATCA GTACATTCCC 
CCAGATCAGA AACAAGAACC CGACCAAGCC CCGATGGACT CCTCGTATGC TCGTTTACTA 
CAACAACAGC AGCTATTTTT GCAGCTACAG ATCCTGAGCC AACAGCAACA GCAGCACTAC 
AACTACCAGA CCATCCTTCC TGCACCTCTC AAGCCTGTGA TTGAAGGCCA AAACAGCAGT 
GCCACAGTGG CTATCAACAA TGCCAATAGC CTTCCTGCCT CGATTGTAGT TTCTCTGCCC 
ACAACAACAC CTGTGCGGCC CAATAGTACT CTGTCCAACC GCAAAGCTGG CATATTACCA 
GCCAACCTGG AGGAGATGAA GGTGGCAGAG CTGAAAATGG AGTTGAAGTT GCGTGGTCTT 
CCAGTATCAG GAACAAAAAC TGACCTTATT GAACGGCTCA AGCCTTTCCA AGAAAATCCT 
ATCACTACTG CACCCAACAA CTCCTCCACA GGACCTAAAA CACAGCCCTG CGGTACACCT 
ATGGAAGTTT CCAGCACCAC TACAACCATC TCCCCCATCC AACTCCCCTC TAATACTCTG 
AGTTCCACTC CACCAGTGTC TCCCAGTTCC TCAGAGCTTC TCAGCAGGGA GGACATGTCC 
GTGGAAGGAC AAGCAGAGAC TCAAAACCGC TGTTTAATTA AAGCAAATGC TGTTCCTGAG 
GAACAAGATC GGAGACTGCA CGAAAAGGAG CGACAGATTG AGGAATTGCT GCGTAAATTG 
GAGCAGGAGC AGAAACTGGT GGAAGAACTA AAGATGCAGC TAGAAGTGGA GAAACGAAGT 
GGAGTTGTTC CATCTGTGGA AAATAACAAC GTTTCTGGCC CAAGCATCAT GTTGGCATCT 
GTCAAAGCAG AAAACACTGT CCCTCCCAAC TGCAAACTGA ATTCACACAC TACAACAACT 
CTGGTTAAGC TTGAGGAGGC CCATCCCAAC CAGGTGACAA CAGCCCCGCT CCGTCAGTTC 
ATTATCAGCC ATCAGGGGGT TCCACAAGTC ATCGGGCAGC CCCAAACACT TCTAACCACT 
CAACATGGGG GCACTCAGAT CCTTCTTCCA ATGCCCCTGG CGAATAATAC CACTACCATC 
CAGCTGCCTA ATGCTAATGT CAAACTACAG CCAGTCTTGC AAGCTGTTTC TACACCAGGC 
ACAGGCCTAA TTCAGACGCC ACAGCTGCAG TCTACCAAAG CAGAAAGTCC TACTTCACAA 
CAGCTTCTCA GTCACAACCA CATAGTTCAG ACCCTGTCTG TGGGCACCAC TGAGAGGTCA 
GGTTTCCAGC ACAGTACCAG TGAGAACCAG GCAGGCCTGG AGATTCCTCA GTGTTTCCTC 
AGTAGCTCCC CAGAGAACAG AACCTCACCT CAGACGTCCC CCATCCCATC ATCCCTTATC 
AATGGACAAC TAAACAAGGC CTCTTTTATC CAGCAGTGCC CAGTTTTCAA CCAGGCTCCC 
AAGAACAGAG AGCCACCCCG TTATGAAGAG GCCGTCAAAC AAACACGCAG CCTCCAGAAT 
CCCACCATCT CGGAGTTTCC CACAGTGACG AGTCAACAGA TGGATGATCT TTTTGACATT 
TTAATAGAAA GTGGAGAGAT TACACCCTTT AGTCAACAGG ACCCATCTGT GCCTAAGATG 
ATGCCAGTCA CGGCCAGCAT CACCACTCTA CCTATTAGCA CAGCCCTGTC CCGACCGCCC 
GCACAGGTGC AGATGGCACC CCCTCCAACC CTCATGGTGG AGGCCTTGCC CAGCCTGGCC 
TCCCTGGACT CCGACAACCA ATTAGAGGCT CTGCTGGAGG GCACATTAGT CGGGGACACA 
GAGCCAGAGC AGAGGACGTT GTGCCTGCTA GAAGAGCTCC AAAACCAGAT GCTGGACCAA 
CCAAACTCCC CAATGGACAC GTCCGAGCTT GGTTTCAGCG ACCCGCCCAC TCAGTCCTCA 
TTCAGTCTGC AAGACACCGG CCTGGACAAC ATGGAGTGGC TGGACCTAAC TATGCCAGGG 
CCCATTGGTG GACTCAACCC ACTGGGCATC GCCTCTGAAT TCCTGGACAC TCATGACCTG 
CAGCTCCACT GGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304979.1
CDS	285..3242
Translation	MEEQGDAAIGGLLAPSPQSEAVTHEMGELSLHPSQRLPPLNERKNVLQLRLQQRRTREQLVEQGIMPPLKSPAAFHEQIRSLERARTENFLKHKIRSRPERAELVRMHILQETLAEPSLQATQLRLKRARLADDLNEKIAQRPGPMELVEKNILPVDSSLKEAIIDGQMQYPRTLEDLIDEDSGDAFSPEQPASQESQGSAASPAEVRTMEETSPLPNNLLQHFPAVTTTSTDFLKPFSTVEQSVSPPAPIPQLVTVAPVKSGPTLVKSQPKLPGDKSRSKKSKESKPRVKKLKYHQYIPPDQKQEPDQAPMDSSYARLLQQQQLFLQLQILSQQQQQHYNYQTILPAPLKPVIEGQNSSATVAINNANSLPASIVVSLPTTTPVRPNSTLSNRKAGILPANLEEMKVAELKMELKLRGLPVSGTKTDLIERLKPFQENPITTAPNNSSTGPKTQPCGTPMEVSSTTTTISPIQLPSNTLSSTPPVSPSSSELLSREDMSVEGQAETQNRCLIKANAVPEEQDRRLHEKERQIEELLRKLEQEQKLVEELKMQLEVEKRSGVVPSVENNNVSGPSIMLASVKAENTVPPNCKLNSHTTTTLVKLEEAHPNQVTTAPLRQFIISHQGVPQVIGQPQTLLTTQHGGTQILLPMPLANNTTTIQLPNANVKLQPVLQAVSTPGTGLIQTPQLQSTKAESPTSQQLLSHNHIVQTLSVGTTERSGFQHSTSENQAGLEIPQCFLSSSPENRTSPQTSPIPSSLINGQLNKASFIQQCPVFNQAPKNREPPRYEEAVKQTRSLQNPTISEFPTVTSQQMDDLFDILIESGEITPFSQQDPSVPKMMPVTASITTLPISTALSRPPAQVQMAPPPTLMVEALPSLASLDSDNQLEALLEGTLVGDTEPEQRTLCLLEELQNQMLDQPNSPMDTSELGFSDPPTQSSFSLQDTGLDNMEWLDLTMPGPIGGLNPLGIASEFLDTHDLQLHWD

Target ORF information:

RefSeq Version	XM_009306704.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio MKL/myocardin-like 2a (mkl2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306704.3

ORF Insert Sequence:

ATGGAGGAAC AGGGGGATGC TGCGATTGGG GGACTGTTGG CACCCAGCCC ACAGAGTGAG 
GCTGTGACCC ATGAGATGGG TGAACTGTCC CTGCACCCCA GCCAGAGGCT TCCTCCTCTC 
AATGAGCGCA AAAATGTTCT CCAGCTGAGG CTCCAGCAGA GACGCACCCG GGAGCAGCTC 
GTTGAACAGG GCATCATGCC ACCCCTGAAG AGCCCTGCAG CTTTCCATGA ACAGATTCGC 
AGCCTTGAGA GAGCCAGGAC TGAAAATTTC CTAAAGCACA AAATACGCAG CCGCCCAGAA 
AGGGCTGAGT TGGTCCGCAT GCACATCTTA CAAGAGACCT TGGCAGAGCC TTCACTGCAG 
GCCACCCAGT TGAGACTAAA GAGAGCTCGA CTGGCTGATG ACCTCAATGA GAAGATTGCA 
CAGCGCCCCG GCCCAATGGA GCTGGTGGAA AAGAACATCC TGCCAGTGGA CTCCAGCCTC 
AAGGAGGCCA TAATTGATGG TCAGATGCAG TACCCTAGAA CTCTAGAAGA TCTAATAGAT 
GAAGACAGTG GAGATGCTTT TTCACCAGAG CAGCCAGCCA GTCAGGAGTC CCAGGGATCT 
GCAGCCTCAC CTGCAGAGGT CAGGACTATG GAGGAGACCT CACCGCTGCC CAATAACTTG 
CTACAGCACT TTCCTGCAGT CACCACTACT TCGACAGATT TCCTCAAGCC CTTTTCCACC 
GTTGAACAAT CCGTTAGCCC TCCAGCTCCC ATACCACAAC TAGTCACAGT AGCCCCTGTG 
AAGTCGGGGC CCACCCTTGT AAAGAGTCAG CCCAAGTTAC CTGGAGACAA GAGCCGCAGC 
AAGAAGAGCA AGGAGTCCAA GCCACGTGTG AAAAAGTTGA AATATCATCA GTACATTCCC 
CCAGATCAGA AACAAGAACC CGACCAAGCC CCGATGGACT CCTCGTATGC TCGTTTACTA 
CAACAACAGC AGCTATTTTT GCAGCTACAG ATCCTGAGCC AACAGCAACA GCAGCACTAC 
AACTACCAGA CCATCCTTCC TGCACCTCTC AAGCCTGTGA TTGAAGGCCA AAACAGCAGT 
GCCACAGTGG CTATCAACAA TGCCAATAGC CTTCCTGCCT CGATTGTAGT TTCTCTGCCC 
ACAACAACAC CTGTGCGGCC CAATAGTACT CTGTCCAACC GCAAAGCTGG CATATTACCA 
GCCAACCTGG AGGAGATGAA GGTGGCAGAG CTGAAAATGG AGTTGAAGTT GCGTGGTCTT 
CCAGTATCAG GAACAAAAAC TGACCTTATT GAACGGCTCA AGCCTTTCCA AGAAAATCCT 
ATCACTACTG CACCCAACAA CTCCTCCACA GGACCTAAAA CACAGCCCTG CGGTACACCT 
ATGGAAGTTT CCAGCACCAC TACAACCATC TCCCCCATCC AACTCCCCTC TAATACTCTG 
AGTTCCACTC CACCAGTGTC TCCCAGTTCC TCAGAGCTTC TCAGCAGGGA GGACATGTCC 
GTGGAAGGAC AAGCAGAGAC TCAAAACCGC TGTTTAATTA AAGCAAATGC TGTTCCTGAG 
GAACAAGATC GGAGACTGCA CGAAAAGGAG CGACAGATTG AGGAATTGCT GCGTAAATTG 
GAGCAGGAGC AGAAACTGGT GGAAGAACTA AAGATGCAGC TAGAAGTGGA GAAACGAAGT 
GGAGTTGTTC CATCTGTGGA AAATAACAAC GTTTCTGGCC CAAGCATCAT GTTGGCATCT 
GTCAAAGCAG AAAACACTGT CCCTCCCAAC TGCAAACTGA ATTCACACAC TACAACAACT 
CTGGTTAAGC TTGAGGAGGC CCATCCCAAC CAGGTGACAA CAGCCCCGCT CCGTCAGTTC 
ATTATCAGCC ATCAGGGGGT TCCACAAGTC ATCGGGCAGC CCCAAACACT TCTAACCACT 
CAACATGGGG GCACTCAGAT CCTTCTTCCA ATGCCCCTGG CGAATAATAC CACTACCATC 
CAGCTGCCTA ATGCTAATGT CAAACTACAG CCAGTCTTGC AAGCTGTTTC TACACCAGGC 
ACAGGCCTAA TTCAGACGCC ACAGCTGCAG TCTACCAAAG CAGAAAGTCC TACTTCACAA 
CAGCTTCTCA GTCACAACCA CATAGTTCAG ACCCTGTCTG TGGGCACCAC TGAGAGGTCA 
GGTTTCCAGC ACAGTACCAG TGAGAACCAG GCAGGCCTGG AGATTCCTCA GTGTTTCCTC 
AGTAGCTCCC CAGAGAACAG AACCTCACCT CAGACGTCCC CCATCCCATC ATCCCTTATC 
AATGGACAAC TAAACAAGGC CTCTTTTATC CAGCAGTGCC CAGTTTTCAA CCAGGCTCCC 
AAGAACAGAG AGCCACCCCG TTATGAAGAG GCCGTCAAAC AAACACGCAG CCTCCAGAAT 
CCCACCATCT CGGAGTTTCC CACAGTGACG AGTCAACAGA TGGATGATCT TTTTGACATT 
TTAATAGAAA GTGGAGAGAT TACACCCTTT AGTCAACAGG ACCCATCTGT GCCTAAGATG 
ATGCCAGTCA CGGCCAGCAT CACCACTCTA CCTATTAGCA CAGCCCTGTC CCGACCGCCC 
GCACAGGTGC AGATGGCACC CCCTCCAACC CTCATGGTGG AGGCCTTGCC CAGCCTGGCC 
TCCCTGGACT CCGACAACCA ATTAGAGGCT CTGCTGGAGG GCACATTAGT CGGGGACACA 
GAGCCAGAGC AGAGGACGTT GTGCCTGCTA GAAGAGCTCC AAAACCAGAT GCTGGACCAA 
CCAAACTCCC CAATGGACAC GTCCGAGCTT GGTTTCAGCG ACCCGCCCAC TCAGTCCTCA 
TTCAGTCTGC AAGACACCGG CCTGGACAAC ATGGAGTGGC TGGACCTAAC TATGCCAGGG 
CCCATTGGTG GACTCAACCC ACTGGGCATC GCCTCTGAAT TCCTGGACAC TCATGACCTG 
CAGCTCCACT GGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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mkl2a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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電話

FAX

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