zgc:171445 cDNA ORF clone, Danio rerio(zebrafish)

The following zgc:171445 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the zgc:171445 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa36414 NM_001113629.1
Latest version!
Danio rerio zgc:171445 (zgc:171445), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa36414
    Clone ID Related Accession (Same CDS sequence) NM_001113629.1
    Accession Version NM_001113629.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4380bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-07-02
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein LOC793035 precursor
    Comment Comment: PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from BC154439.1. On Jan 20, 2008 this sequence version replaced XM_001332142.1. ##Evidence-Data-START## Transcript exon combination :: BC154439.1, BC171612.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA3505370 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGATGA TGGAGATCAG TGTTTGGAAG TGGATCAATC TAGTCCTTTT CTTCGGTTTT 
    GTTGATGGTG CCAAGCTTAT TGTTGAAGCC CCAAAACCAA TAGAACCGTT GGTCAATGCT
    GATGAACAGA TCTCAGGGCC GCTCTTCCTG GTGACGTTTC CTTCATTGAT TGAGTCCGGC
    TCTGGTGCTA AACTGTGCGC TAGTCTTCTG AAGCCCCAAG GCAGTCTCAC AATAACCATT
    ACTCTGATAG ATGACAAAAG CGCAGAAACT CGACTCGTGC GTCAGACAAC CCAGAGGAAG
    CTTCACAGAT GTTTTAATTT CCAGGCTCCT CTTGTAAATG GAGATTCGGT GCAGACTGTG
    AGGGTGGTTG TTCAAGGACA GTCCTTCAAA ATGACTGAAG AGCGTAAAGT GGTGTTCAGG
    TCTTACCTGC CTCTGACCTT CATCCAGACA GACAAACCGC TCTACAATCC AGGACAGACA
    GTGAATTTCA GAGTCGTCAC CATGAGTGAT AAACTTGTGC CTCTTGACCA AATGTACAAT
    CTGGTGGTGT TGGAGGACTT TCGTAATATC CGGATAAATC AGTGGACAAA TGTTTCCTCC
    GTGAACTGGA TTTTGGAGCT TTCTCATGTT TTGATCCCAG AAGCTCAGGT TGGGACGTAC
    ACACTGAGGG CTTATATTGG TGACCGAATC ATCTCCCAGA GTTTTGATGT GAAACAATAC
    GTTTTACCCA AGTTTGATGT GACCGTAAAC ACACTGCAGA TCTATAGTGT TGCAGATGCG
    GGACTGAAAG TTGAGGCTTG TGGAAAGTAC ACATTTGGGC AGCCTGTACC TGGTCAAGTG
    CTAGTGCAAG TGTGCCGTCA GCCTCTTGCC TTCATTCAGG ATCCTAAAGT GACCAGCATT
    TGTCTGAATA AATCCACGAG GACGAACAAC ACTGGCTGTG CCTCTCTAAC ACTCGGTACA
    TCATCGTTTT TTGGAACCAG TTTTGAGAGT AACCTGCAAA ATTCCTTCAT TGTTAATGTC
    AACCTTACTG AGGAGGGAAC AGGCATTGTC ATGTCGAAAT CCACAACGGT GTCGATTACA
    TTTGAAGTTG GCAAGGTAAC ATTTGTGGAT CTCCCAAAGT TCTACAATTA TGGGTCAACG
    GTGAATGGCA AGATCTCCGT GTCTGATTTC AATGGGAACC CGATCTATAA CAAGGCCGTG
    TATCTCCTGG ACAGCAGCAT ATGGCCCAAC AAAGTGCTGT TCAATTTGAC CACGAACTAT
    AATGGATTTG CCAAGTTCTC CCTTGACACG ACTAGTTTTC CTCAAGCTGA TCTGAATCTT
    GTGGCAAGTG CAACTCCACA GTCTTATTTC AGTTACTATT CGCCATACTT CACTACAGAT
    TCACAGGTGG TTCAGCTTTC CCAAACTGAT CCTGACACCC CAACCTTTAG TGATCTGTCT
    ATAGTGAAGC TTGAGCAGCC GCTGAAGTGT GGCACCACTT ATTCAGTGAC TGTCAAGTAT
    TTATTTGTTG GAGAGACTGG CGACTACAGT GCTGACATCA TCTATATGGT CATGTCCAGA
    GGAGTGATCG TTCTGCATGG GTTTAAAACT GTTCAAGCTC GGGCTTCTAA TACCCTCACG
    AAGGGCACGG TGACGTTTGA TCTCTCAATT CTTGCCAATA TGGCTCCGAT GGTTCAGATT
    CTGGTGTACA GTGTTCTGCC CAGTCAGACT GTAGTGACCG GTAGTGCATC GTTTGACACG
    GAGAAGTGCT TCTCAAACCA GGTTTCTCTG CAGTTCTCTC CTGCTACAGC GGTTCCTGGT
    GAGGGAAGTA TTTTGACCGT CTCCGCTCAA GCCGGATCTC TGTGTGGCCT CAGTGCTATA
    GATCAGAGTG TTCTGATCAT GCAGTCAGGA GGACGTCTGA GCGCAGAGGC GGTGTTTAAC
    ATGCTCCCAC TGCAATCACT ATCGGATTAT CCATACGGTG CTGAAGATCA ACAAGACTGC
    CTAAATGTTC GACCCCGTCG AGCTGTTCCA ACAGATCAAG CTTATAACAC CTTCAAGAGT
    GTGGGGATGA AGATTGCAAC AAATCTGCCA GTTCGAGAGC CTGAGTGCCT GAAGTTCAAA
    GACCTGCTTT ACTATCGCAA CTTCCATAGG GTAATTCCTT TTCGGGGTAC TGGTTTTGCT
    ATGGCTGAAA TGGCTCCAGC TCCTTTAGCA ATGGCAGAAA TTGGAGGAAC TAGAGGTTCC
    TCTGTTGATG TGACCATCAG AACTTACTTT CCAGAAACCT GGATCTGGCA ACTTGCTACT
    GTGGGAGACT CTGGATCCAC ATCAGTTCCT CTCACGGTTC CTGATACGAC CACCACATGG
    GACACTGAAG CCTTCTGTCT CTCCTCCAAT GGTTTTGGTC TGGCTCCACC AGTTCTGCTG
    ACTTCCTTCC AGCCCTTCTT CCTAGAGCTC ACCCTGCCTT ACTCCATTAT CCGTGGGGAG
    GCTTTTGAGC TGGTGGCCAC GGTCTTCAAC TATCTGTTGC AATGCATCAA GATTCAAGTG
    ACTCCAACAC CGTCCTCCAA CTACACTCTA ACACCCATCA ATGATCAGTC TTCATCTACT
    CTCTCTACCA ATTGGAGAAA AACCATCAAA TGGGCTTTAA CTGCTTCTGT TATTGGAACC
    GTAAACGTGA CCATTAGTGC TGAGGCCAGT CCATCCCAGG AATTGTGCGG TGATCAGGAT
    GTGACAGTAC TATCAAGAGG ACGCATTGAC ATAGTCACTC GAAGTCTGCG GGTTCTGGCT
    GAAGGAGTGG AAAGGACCTT CACACGGAGT TGGTTACTCT GTCCAAAGGG AAGCATGCTT
    TCAGAGAGCG TGAAGATCAC ACTTCCAAAA AGTGTCATCC AGGGATCAGC CAGCTGCTCT
    GTATCAGTCA TTGGGGACAT CATGGGTCGT GCACTGAACA ACCTGGCCAA TTTGCTACAG
    ATGCCTTCTA GCTGTGGAGA ACAGAACATG ATCATTCTCG CTCCCAATGT TTACATCCTA
    CGGTATCTGA CAGTAACTGC ACAGCTCACT CCAGCCATCC AAGACACTGC CACGAGTTAT
    CTTCTGACTG GATATCAAGG AGAGCTGAAC TACAGGCACA GTGATGGTTC ATTCAGCACT
    TTTGGTTATG ATGCATCCAA TACATGGTTG ACTGCCTTTG TCATGAGGAC TTTTGGTCTA
    GCAAGGAGCT TCACCTACAT AGATCCCAAT GTTCTTAAGG GTGCAAAGGA CTGGTTGATT
    GGCACGCAGG GTTCAGATGG CTGTTTCGTG CAGCAGGGCA CTCTGTACCA CAATGACATG
    AAGGGTGGAG TTGGTGATAA CGTGACCATG ACAGGCTACA TTGTTGCATC ACTGCTTGAA
    ATAGGCGTCC CTGCCACGGA TCCTGTCATT ACGAAGGCTC TGTCGTTTTT GAGGCCTCTT
    GTTGGGAATC TGGGAAACGC CTATGTCACT GCTCTGCTGG CCTACACCTT CAGTCTGGCT
    GGAGAGACCA GCACTCGAGC ACAGCTTTTT AGTTCCTTGA GAAACACTGC CATTTCTGAA
    GGTACTACTC TGCACTGGTC TCAGACTACA TCTGGGGATA CTCTGGCAGT GGAGATCAGT
    GCATATGTGC TGTTAGCGGA TCTCACTGTA CAGCCTGTGA CCACTGCTAA TCTGGGCTAT
    GCTAACCGCA TTGTCAGCTG GATTGTGGCC CAGCAGAATC CCTATGGTGG TTTCTCCTCC
    ACTCAAGACA CTGTGGTGGC TCTTCAGGCC TTGGCTCTGT ATGCTGCTCA AGCGTTCACC
    CCTGGAGGCT CCAGCACAGT TACAGTTCAG TCCTCAGTGC CAGCAGGAGA CGTTTTCAAC
    TTTGCTGTGA CTCCCAACAA CCGCCTGCTG TACCAGAGAA ATTCACTGAA CAACTTCCCT
    GGCACATATA GTGTTGTAGC AAGGGGATCT GCCTGTGCTT CTGTGCAGGT TGCCTGTTTC
    TACAACATCC CAACTCCGGT TACAGTTGCC AGAACCTTGA GTGTTGTTGC TAAGGTGACC
    GGGGACTGCC AGGCTGCACC AGTCAATCTG ATGTTGACCT TCACAGTCAA ATACAATGGT
    CGAAAGCCAG CTACTAACAT GGTTCTGGTG GACATCAAGG TGTTATCAGG ATTCACTGCA
    GATACCTCAC TGCTTGGATC CCCACCTAAT TTTGCTCCAT TAGTGCAGAG GGTTGATTCT
    AATGGTGACC ATGTCCTTGT CTATCTACAA GAGGTTCCCA AAGGTGTTCC AGTGACCTTC
    AGTATACAGC TGACACAGAC TGTTGCAGTG CAGAATCTCA AGCCAGCGGT CATCAATATC
    TATGACTATT ACCAGAGAAG TGATAAATTT GAGACCACAT ACAAATCCCC CTGTCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001107101.1
    CDS93..4472
    Translation

    Target ORF information:

    RefSeq Version NM_001113629.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio zgc:171445 (zgc:171445), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001113629.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGATGA TGGAGATCAG TGTTTGGAAG TGGATCAATC TAGTCCTTTT CTTCGGTTTT 
    GTTGATGGTG CCAAGCTTAT TGTTGAAGCC CCAAAACCAA TAGAACCGTT GGTCAATGCT
    GATGAACAGA TCTCAGGGCC GCTCTTCCTG GTGACGTTTC CTTCATTGAT TGAGTCCGGC
    TCTGGTGCTA AACTGTGCGC TAGTCTTCTG AAGCCCCAAG GCAGTCTCAC AATAACCATT
    ACTCTGATAG ATGACAAAAG CGCAGAAACT CGACTCGTGC GTCAGACAAC CCAGAGGAAG
    CTTCACAGAT GTTTTAATTT CCAGGCTCCT CTTGTAAATG GAGATTCGGT GCAGACTGTG
    AGGGTGGTTG TTCAAGGACA GTCCTTCAAA ATGACTGAAG AGCGTAAAGT GGTGTTCAGG
    TCTTACCTGC CTCTGACCTT CATCCAGACA GACAAACCGC TCTACAATCC AGGACAGACA
    GTGAATTTCA GAGTCGTCAC CATGAGTGAT AAACTTGTGC CTCTTGACCA AATGTACAAT
    CTGGTGGTGT TGGAGGACTT TCGTAATATC CGGATAAATC AGTGGACAAA TGTTTCCTCC
    GTGAACTGGA TTTTGGAGCT TTCTCATGTT TTGATCCCAG AAGCTCAGGT TGGGACGTAC
    ACACTGAGGG CTTATATTGG TGACCGAATC ATCTCCCAGA GTTTTGATGT GAAACAATAC
    GTTTTACCCA AGTTTGATGT GACCGTAAAC ACACTGCAGA TCTATAGTGT TGCAGATGCG
    GGACTGAAAG TTGAGGCTTG TGGAAAGTAC ACATTTGGGC AGCCTGTACC TGGTCAAGTG
    CTAGTGCAAG TGTGCCGTCA GCCTCTTGCC TTCATTCAGG ATCCTAAAGT GACCAGCATT
    TGTCTGAATA AATCCACGAG GACGAACAAC ACTGGCTGTG CCTCTCTAAC ACTCGGTACA
    TCATCGTTTT TTGGAACCAG TTTTGAGAGT AACCTGCAAA ATTCCTTCAT TGTTAATGTC
    AACCTTACTG AGGAGGGAAC AGGCATTGTC ATGTCGAAAT CCACAACGGT GTCGATTACA
    TTTGAAGTTG GCAAGGTAAC ATTTGTGGAT CTCCCAAAGT TCTACAATTA TGGGTCAACG
    GTGAATGGCA AGATCTCCGT GTCTGATTTC AATGGGAACC CGATCTATAA CAAGGCCGTG
    TATCTCCTGG ACAGCAGCAT ATGGCCCAAC AAAGTGCTGT TCAATTTGAC CACGAACTAT
    AATGGATTTG CCAAGTTCTC CCTTGACACG ACTAGTTTTC CTCAAGCTGA TCTGAATCTT
    GTGGCAAGTG CAACTCCACA GTCTTATTTC AGTTACTATT CGCCATACTT CACTACAGAT
    TCACAGGTGG TTCAGCTTTC CCAAACTGAT CCTGACACCC CAACCTTTAG TGATCTGTCT
    ATAGTGAAGC TTGAGCAGCC GCTGAAGTGT GGCACCACTT ATTCAGTGAC TGTCAAGTAT
    TTATTTGTTG GAGAGACTGG CGACTACAGT GCTGACATCA TCTATATGGT CATGTCCAGA
    GGAGTGATCG TTCTGCATGG GTTTAAAACT GTTCAAGCTC GGGCTTCTAA TACCCTCACG
    AAGGGCACGG TGACGTTTGA TCTCTCAATT CTTGCCAATA TGGCTCCGAT GGTTCAGATT
    CTGGTGTACA GTGTTCTGCC CAGTCAGACT GTAGTGACCG GTAGTGCATC GTTTGACACG
    GAGAAGTGCT TCTCAAACCA GGTTTCTCTG CAGTTCTCTC CTGCTACAGC GGTTCCTGGT
    GAGGGAAGTA TTTTGACCGT CTCCGCTCAA GCCGGATCTC TGTGTGGCCT CAGTGCTATA
    GATCAGAGTG TTCTGATCAT GCAGTCAGGA GGACGTCTGA GCGCAGAGGC GGTGTTTAAC
    ATGCTCCCAC TGCAATCACT ATCGGATTAT CCATACGGTG CTGAAGATCA ACAAGACTGC
    CTAAATGTTC GACCCCGTCG AGCTGTTCCA ACAGATCAAG CTTATAACAC CTTCAAGAGT
    GTGGGGATGA AGATTGCAAC AAATCTGCCA GTTCGAGAGC CTGAGTGCCT GAAGTTCAAA
    GACCTGCTTT ACTATCGCAA CTTCCATAGG GTAATTCCTT TTCGGGGTAC TGGTTTTGCT
    ATGGCTGAAA TGGCTCCAGC TCCTTTAGCA ATGGCAGAAA TTGGAGGAAC TAGAGGTTCC
    TCTGTTGATG TGACCATCAG AACTTACTTT CCAGAAACCT GGATCTGGCA ACTTGCTACT
    GTGGGAGACT CTGGATCCAC ATCAGTTCCT CTCACGGTTC CTGATACGAC CACCACATGG
    GACACTGAAG CCTTCTGTCT CTCCTCCAAT GGTTTTGGTC TGGCTCCACC AGTTCTGCTG
    ACTTCCTTCC AGCCCTTCTT CCTAGAGCTC ACCCTGCCTT ACTCCATTAT CCGTGGGGAG
    GCTTTTGAGC TGGTGGCCAC GGTCTTCAAC TATCTGTTGC AATGCATCAA GATTCAAGTG
    ACTCCAACAC CGTCCTCCAA CTACACTCTA ACACCCATCA ATGATCAGTC TTCATCTACT
    CTCTCTACCA ATTGGAGAAA AACCATCAAA TGGGCTTTAA CTGCTTCTGT TATTGGAACC
    GTAAACGTGA CCATTAGTGC TGAGGCCAGT CCATCCCAGG AATTGTGCGG TGATCAGGAT
    GTGACAGTAC TATCAAGAGG ACGCATTGAC ATAGTCACTC GAAGTCTGCG GGTTCTGGCT
    GAAGGAGTGG AAAGGACCTT CACACGGAGT TGGTTACTCT GTCCAAAGGG AAGCATGCTT
    TCAGAGAGCG TGAAGATCAC ACTTCCAAAA AGTGTCATCC AGGGATCAGC CAGCTGCTCT
    GTATCAGTCA TTGGGGACAT CATGGGTCGT GCACTGAACA ACCTGGCCAA TTTGCTACAG
    ATGCCTTCTA GCTGTGGAGA ACAGAACATG ATCATTCTCG CTCCCAATGT TTACATCCTA
    CGGTATCTGA CAGTAACTGC ACAGCTCACT CCAGCCATCC AAGACACTGC CACGAGTTAT
    CTTCTGACTG GATATCAAGG AGAGCTGAAC TACAGGCACA GTGATGGTTC ATTCAGCACT
    TTTGGTTATG ATGCATCCAA TACATGGTTG ACTGCCTTTG TCATGAGGAC TTTTGGTCTA
    GCAAGGAGCT TCACCTACAT AGATCCCAAT GTTCTTAAGG GTGCAAAGGA CTGGTTGATT
    GGCACGCAGG GTTCAGATGG CTGTTTCGTG CAGCAGGGCA CTCTGTACCA CAATGACATG
    AAGGGTGGAG TTGGTGATAA CGTGACCATG ACAGGCTACA TTGTTGCATC ACTGCTTGAA
    ATAGGCGTCC CTGCCACGGA TCCTGTCATT ACGAAGGCTC TGTCGTTTTT GAGGCCTCTT
    GTTGGGAATC TGGGAAACGC CTATGTCACT GCTCTGCTGG CCTACACCTT CAGTCTGGCT
    GGAGAGACCA GCACTCGAGC ACAGCTTTTT AGTTCCTTGA GAAACACTGC CATTTCTGAA
    GGTACTACTC TGCACTGGTC TCAGACTACA TCTGGGGATA CTCTGGCAGT GGAGATCAGT
    GCATATGTGC TGTTAGCGGA TCTCACTGTA CAGCCTGTGA CCACTGCTAA TCTGGGCTAT
    GCTAACCGCA TTGTCAGCTG GATTGTGGCC CAGCAGAATC CCTATGGTGG TTTCTCCTCC
    ACTCAAGACA CTGTGGTGGC TCTTCAGGCC TTGGCTCTGT ATGCTGCTCA AGCGTTCACC
    CCTGGAGGCT CCAGCACAGT TACAGTTCAG TCCTCAGTGC CAGCAGGAGA CGTTTTCAAC
    TTTGCTGTGA CTCCCAACAA CCGCCTGCTG TACCAGAGAA ATTCACTGAA CAACTTCCCT
    GGCACATATA GTGTTGTAGC AAGGGGATCT GCCTGTGCTT CTGTGCAGGT TGCCTGTTTC
    TACAACATCC CAACTCCGGT TACAGTTGCC AGAACCTTGA GTGTTGTTGC TAAGGTGACC
    GGGGACTGCC AGGCTGCACC AGTCAATCTG ATGTTGACCT TCACAGTCAA ATACAATGGT
    CGAAAGCCAG CTACTAACAT GGTTCTGGTG GACATCAAGG TGTTATCAGG ATTCACTGCA
    GATACCTCAC TGCTTGGATC CCCACCTAAT TTTGCTCCAT TAGTGCAGAG GGTTGATTCT
    AATGGTGACC ATGTCCTTGT CTATCTACAA GAGGTTCCCA AAGGTGTTCC AGTGACCTTC
    AGTATACAGC TGACACAGAC TGTTGCAGTG CAGAATCTCA AGCCAGCGGT CATCAATATC
    TATGACTATT ACCAGAGAAG TGATAAATTT GAGACCACAT ACAAATCCCC CTGTCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.