ndst1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ndst1b
Entrez Gene ID	570459
Full Name	N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1b
Synonyms	fj47c03,ndst1,wu:fj47c03
General protein information	Preferred Names N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1b Names bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001324455.1	NP_001311384.1	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1
XM_009295591.2	XP_009293866.1	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 isoform X1
XM_009295591.3	XP_009293866.1	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 isoform X1
XM_017352818.1	XP_017208307.1	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

The NDST gene family in zebrafish: role of NDST1B in pharyngeal arch formation.
Filipek-G?rniok B, Carlsson P, Haitina T, Habicher J, Ledin J, Kjell?n L
PloS one10(3)e0119040(2015)

Evolution of the miR199-214 cluster and vertebrate skeletal development.
Desvignes T, Contreras A, Postlethwait JH
RNA biology11(4)281-94(2014)

Glycome and transcriptome regulation of vasculogenesis.
Harfouche R, Hentschel DM, Piecewicz S, Basu S, Print C, Eavarone D, Kiziltepe T, Sasisekharan R, Sengupta S
Circulation120(19)1883-1892(2009 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ndst1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ndst1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa45974	NM_001324455.1 Latest version!	Danio rerio N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1b (ndst1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa45974	XM_009295591.3 Latest version!	Danio rerio N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1b (ndst1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa45974	XM_017352818.1 Latest version!	Danio rerio N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1b (ndst1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa45974	XM_009295591.2	Danio rerio N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1b (ndst1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ndst1b ( NM_001324455.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001311384.1 Danio rerio N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1b (ndst1b), mRNA.

Price & Availability↑

CloneID	ODa45974
Clone ID Related Accession (Same CDS sequence)	NM_001324455.1 , XM_009295591.2 , XM_009295591.3 , XM_017352818.1
Accession Version	NM_001324455.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-23
Organism	Danio rerio(zebrafish)
Product	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX072577.14 and LO018008.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01024377.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGATGCTGA TGTGTGGACG TGTAAGGCGG CTCTTCCGTC AGCTCACGCT GCAGACCAGC 
CTGCTGCTGC TCTTTCTCTT TTGTATGGTC AGTGTCCTCA TCTCAGCCTA TTTCCTATAT 
GGCGTCAAAC GGGAGCTTGA ACCAGCTGGA GCCGGAGGTG TGGTTGTCCC TGAAGAAGGT 
ACAGCCGATT GGGAAGATCC TCGCGCAACT CCTTCCCCTC CTTCAGCAAG GGTTCTACCT 
GCCAGAACGG CCAAACCAGC AGACATGTCT CGAACTGATC CTGTAGTGCT TGTGTTCGTT 
GAGAGCTTGT ATTCCCAGTT GGGACAGGAC ATTGTTGCCA TCTTGGAATC TGGAAGATTT 
CGGTATCAGA CAGAGATTGC GCCTGGAAAA GGTGACATGC CAACACTCAC AGATAAGAAC 
CGGGGTCGAT TCACGTTGGT CATCTACGAG AACATCCTCA AGTATGTCAA CCTGGATGCC 
TGGAATAGAG AGTTGCTGGA TAAGTACTGT GTGGAGTACG GCGTTGGCAT CATTGGCTTT 
TTCAAGGCTA ATGAAAACAG TTTGCAGAGC GCCCAGCTTA AGGGCTTTCC TCTCTTCCTG 
CACTCCAATC TGGGTTTAAG GGACTGTAGT GTCAACCCAA AGTCTCCACT GCTGTTCATC 
ACCAAGGCCA GAGAAGTGGA GCATGGACCC TTACCTGGAG ATGACTGGAC TGTCTTCCAG 
TCCAACCATA CCACCTATGA GCCTGTGCTG TTAGCTCGGG GACGGAGTAC GGAGGCCAGC 
GGAGCTCCCG GACCCCTTCA TGCAGCTGTG GTTCAGGATT TGGGTCTTCA TGATGGCATC 
CAGCGGGTAC TCTTCGGCAA CAACCTCAAC TTTTGGCTGC ACAAACTGGT ATTGGTGGAC 
GCAGTGAGTT TCTTGACTGG AAAGCGCCTC TCGTTGGCCC TTGAGCGCTA CATACTGGTG 
GATATTGATG ACATCTTTGT TGGGAAGGAG GGAACACGGA TGAAGGTGTC TGATGTCAAG 
GCTCTACTGG AGACGCAGAA TGAACTTCGA AACCATGTGC CCAATTTCAC CTTCAACTTG 
GGATTCTCTG GAAAGTTCTT TCATGCGGGC ACAGATGAGG AGGATCTTGG AGATGACCTG 
CTTCTGTCTT ATAGTAAGGA GTTCTGGTGG TTCCCACACA TGTGGAGTCA CATGCAGCCG 
CATCTATTTC ACAACCAGTC GGTGCTGGCT GAACAAATGC TGCTCAACCA CAAGTTTGCA 
GAGGAGCATG GCATTCCTAC AAACATGGGC TATGCAGTGG CTCCCCATCA CTCTGGAGTG 
TATCCTATCC ATCTGCAGCT GTACGAGGCC TGGAAGAAAG TGTGGGGCAT CAAAGTGACC 
AGTACAGAGG AGTATCCGCA CCTCAAACCT GCACGCTTCC GCAGGGGCTT TGTACACAGT 
GGCATTAGTG TTTTACCCAG ACAGACTTGT GGTCTCTTTA CTCACACCAT CTTCTACAAT 
GAATATCCTG GAGGCTCTAA AGAATTGGAC AAACTCATAT ATGGAGGAGA GCTTTTTCTC 
ACTGTGCTAC TTAACCCAAT CAGCATCTTC ATGACCCACC TGTCCAACTA CGGAAATGAC 
CGCCTTGGCT TGTACACCTT TAAGAACCTG GTGAAGTTCG TCCAGATGTG GACCAATCTA 
AGCCTGCAGA CTCTCCCACC TGTTCAGCTG GCGCAGAAAT ACTTCCAGAT CTTCCCCGAA 
GAGAGAGACC CCATCTGGCA GGACCCCTGT GAGGACAAAA GGCATAAAGA CATCTGGTCT 
AAGGAAAAGA CCTGTGACCG TTTCCCCAAG CTGCTTATCA TTGGACCTCA GAAGACTGGA 
ACCACTGCCC TCTATCTCTT TCTGAGCATG CATTCAGATT TGACCAGTAA CTACCCCAGC 
AAAGAGACCT TCGAGGAAAT CCAGTTCTTC AACGGCCGCA ACTACCACAA AGGAATTGAC 
TGGTACATGG AACATTTCCC TCTACCTTCC AACACTAGCT CAGACTTCTA CTTTGAGAAA 
AGTGCCAACT ACTTTGACTC AGAGGTCGCA GCCCGGCGTG CAGCTGCTCT TCTGCCTAAA 
GCCAAAATCA TCACCATCCT TATCAACCCG GCTGACAGAG CCTATTCCTG GTATCAGCAC 
CAGAGAGCTC ACGATGATCC TGTGGCTCAG AAATATACAT TCCATGATGT CATCACTGCA 
GGCCGGGACG CGCCCATTAA ACTGAGGGTC CTGCAGAGCC GGTGTCTGGT GCCCGGGCTT 
TATGCCACAC ACCTACAGCG GTGGCTCACA CACTATCACC CGAGTCAGAT ATTGGTCCTA 
GATGGTCAGA TGCTGAGGAC AGAACCAGCT TCAGTAATGG ACAAAATCCA GAAGTTTTTA 
GGCCTAATAA ATACACTCAA CTACCACAAG ATACTTGCGT TCGATCCGAA GAAGGGCTTC 
TGGTGTCAGC TGCTGGATGG AGGCAAGACC AAATGCTTGG GGAAGAGTAA AGGGAGGAGA 
TACCCAGACA TGGATGTTGA TTCCCGAACC TTTCTGAGAG AGTATTACCA TGAGCACAAC 
ATTGAACTCT CCAAGCTGCT GTACAAAATG GGTCAGCCGT TGCCCAGCTG GCTAAGAGAG 
GAGCTACTCC ACAGCAGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001311384.1
CDS	738..3398
Translation	MMLMCGRVRRLFRQLTLQTSLLLLFLFCMVSVLISAYFLYGVKRELEPAGAGGVVVPEEGTADWEDPRATPSPPSARVLPARTAKPADMSRTDPVVLVFVESLYSQLGQDIVAILESGRFRYQTEIAPGKGDMPTLTDKNRGRFTLVIYENILKYVNLDAWNRELLDKYCVEYGVGIIGFFKANENSLQSAQLKGFPLFLHSNLGLRDCSVNPKSPLLFITKAREVEHGPLPGDDWTVFQSNHTTYEPVLLARGRSTEASGAPGPLHAAVVQDLGLHDGIQRVLFGNNLNFWLHKLVLVDAVSFLTGKRLSLALERYILVDIDDIFVGKEGTRMKVSDVKALLETQNELRNHVPNFTFNLGFSGKFFHAGTDEEDLGDDLLLSYSKEFWWFPHMWSHMQPHLFHNQSVLAEQMLLNHKFAEEHGIPTNMGYAVAPHHSGVYPIHLQLYEAWKKVWGIKVTSTEEYPHLKPARFRRGFVHSGISVLPRQTCGLFTHTIFYNEYPGGSKELDKLIYGGELFLTVLLNPISIFMTHLSNYGNDRLGLYTFKNLVKFVQMWTNLSLQTLPPVQLAQKYFQIFPEERDPIWQDPCEDKRHKDIWSKEKTCDRFPKLLIIGPQKTGTTALYLFLSMHSDLTSNYPSKETFEEIQFFNGRNYHKGIDWYMEHFPLPSNTSSDFYFEKSANYFDSEVAARRAAALLPKAKIITILINPADRAYSWYQHQRAHDDPVAQKYTFHDVITAGRDAPIKLRVLQSRCLVPGLYATHLQRWLTHYHPSQILVLDGQMLRTEPASVMDKIQKFLGLINTLNYHKILAFDPKKGFWCQLLDGGKTKCLGKSKGRRYPDMDVDSRTFLREYYHEHNIELSKLLYKMGQPLPSWLREELLHSR

Target ORF information:

RefSeq Version	NM_001324455.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1b (ndst1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324455.1

ORF Insert Sequence:

ATGATGCTGA TGTGTGGACG TGTAAGGCGG CTCTTCCGTC AGCTCACGCT GCAGACCAGC 
CTGCTGCTGC TCTTTCTCTT TTGTATGGTC AGTGTCCTCA TCTCAGCCTA TTTCCTATAT 
GGCGTCAAAC GGGAGCTTGA ACCAGCTGGA GCCGGAGGTG TGGTTGTCCC TGAAGAAGGT 
ACAGCCGATT GGGAAGATCC TCGCGCAACT CCTTCCCCTC CTTCAGCAAG GGTTCTACCT 
GCCAGAACGG CCAAACCAGC AGACATGTCT CGAACTGATC CTGTAGTGCT TGTGTTCGTT 
GAGAGCTTGT ATTCCCAGTT GGGACAGGAC ATTGTTGCCA TCTTGGAATC TGGAAGATTT 
CGGTATCAGA CAGAGATTGC GCCTGGAAAA GGTGACATGC CAACACTCAC AGATAAGAAC 
CGGGGTCGAT TCACGTTGGT CATCTACGAG AACATCCTCA AGTATGTCAA CCTGGATGCC 
TGGAATAGAG AGTTGCTGGA TAAGTACTGT GTGGAGTACG GCGTTGGCAT CATTGGCTTT 
TTCAAGGCTA ATGAAAACAG TTTGCAGAGC GCCCAGCTTA AGGGCTTTCC TCTCTTCCTG 
CACTCCAATC TGGGTTTAAG GGACTGTAGT GTCAACCCAA AGTCTCCACT GCTGTTCATC 
ACCAAGGCCA GAGAAGTGGA GCATGGACCC TTACCTGGAG ATGACTGGAC TGTCTTCCAG 
TCCAACCATA CCACCTATGA GCCTGTGCTG TTAGCTCGGG GACGGAGTAC GGAGGCCAGC 
GGAGCTCCCG GACCCCTTCA TGCAGCTGTG GTTCAGGATT TGGGTCTTCA TGATGGCATC 
CAGCGGGTAC TCTTCGGCAA CAACCTCAAC TTTTGGCTGC ACAAACTGGT ATTGGTGGAC 
GCAGTGAGTT TCTTGACTGG AAAGCGCCTC TCGTTGGCCC TTGAGCGCTA CATACTGGTG 
GATATTGATG ACATCTTTGT TGGGAAGGAG GGAACACGGA TGAAGGTGTC TGATGTCAAG 
GCTCTACTGG AGACGCAGAA TGAACTTCGA AACCATGTGC CCAATTTCAC CTTCAACTTG 
GGATTCTCTG GAAAGTTCTT TCATGCGGGC ACAGATGAGG AGGATCTTGG AGATGACCTG 
CTTCTGTCTT ATAGTAAGGA GTTCTGGTGG TTCCCACACA TGTGGAGTCA CATGCAGCCG 
CATCTATTTC ACAACCAGTC GGTGCTGGCT GAACAAATGC TGCTCAACCA CAAGTTTGCA 
GAGGAGCATG GCATTCCTAC AAACATGGGC TATGCAGTGG CTCCCCATCA CTCTGGAGTG 
TATCCTATCC ATCTGCAGCT GTACGAGGCC TGGAAGAAAG TGTGGGGCAT CAAAGTGACC 
AGTACAGAGG AGTATCCGCA CCTCAAACCT GCACGCTTCC GCAGGGGCTT TGTACACAGT 
GGCATTAGTG TTTTACCCAG ACAGACTTGT GGTCTCTTTA CTCACACCAT CTTCTACAAT 
GAATATCCTG GAGGCTCTAA AGAATTGGAC AAACTCATAT ATGGAGGAGA GCTTTTTCTC 
ACTGTGCTAC TTAACCCAAT CAGCATCTTC ATGACCCACC TGTCCAACTA CGGAAATGAC 
CGCCTTGGCT TGTACACCTT TAAGAACCTG GTGAAGTTCG TCCAGATGTG GACCAATCTA 
AGCCTGCAGA CTCTCCCACC TGTTCAGCTG GCGCAGAAAT ACTTCCAGAT CTTCCCCGAA 
GAGAGAGACC CCATCTGGCA GGACCCCTGT GAGGACAAAA GGCATAAAGA CATCTGGTCT 
AAGGAAAAGA CCTGTGACCG TTTCCCCAAG CTGCTTATCA TTGGACCTCA GAAGACTGGA 
ACCACTGCCC TCTATCTCTT TCTGAGCATG CATTCAGATT TGACCAGTAA CTACCCCAGC 
AAAGAGACCT TCGAGGAAAT CCAGTTCTTC AACGGCCGCA ACTACCACAA AGGAATTGAC 
TGGTACATGG AACATTTCCC TCTACCTTCC AACACTAGCT CAGACTTCTA CTTTGAGAAA 
AGTGCCAACT ACTTTGACTC AGAGGTCGCA GCCCGGCGTG CAGCTGCTCT TCTGCCTAAA 
GCCAAAATCA TCACCATCCT TATCAACCCG GCTGACAGAG CCTATTCCTG GTATCAGCAC 
CAGAGAGCTC ACGATGATCC TGTGGCTCAG AAATATACAT TCCATGATGT CATCACTGCA 
GGCCGGGACG CGCCCATTAA ACTGAGGGTC CTGCAGAGCC GGTGTCTGGT GCCCGGGCTT 
TATGCCACAC ACCTACAGCG GTGGCTCACA CACTATCACC CGAGTCAGAT ATTGGTCCTA 
GATGGTCAGA TGCTGAGGAC AGAACCAGCT TCAGTAATGG ACAAAATCCA GAAGTTTTTA 
GGCCTAATAA ATACACTCAA CTACCACAAG ATACTTGCGT TCGATCCGAA GAAGGGCTTC 
TGGTGTCAGC TGCTGGATGG AGGCAAGACC AAATGCTTGG GGAAGAGTAA AGGGAGGAGA 
TACCCAGACA TGGATGTTGA TTCCCGAACC TTTCTGAGAG AGTATTACCA TGAGCACAAC 
ATTGAACTCT CCAAGCTGCT GTACAAAATG GGTCAGCCGT TGCCCAGCTG GCTAAGAGAG 
GAGCTACTCC ACAGCAGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ndst1b ( XM_009295591.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293866.1 Danio rerio N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1b (ndst1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa45974
Clone ID Related Accession (Same CDS sequence)	NM_001324455.1 , XM_009295591.2 , XM_009295591.3 , XM_017352818.1
Accession Version	XM_009295591.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295591.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTGA TGTGTGGACG TGTAAGGCGG CTCTTCCGTC AGCTCACGCT GCAGACCAGC 
CTGCTGCTGC TCTTTCTCTT TTGTATGGTC AGTGTCCTCA TCTCAGCCTA TTTCCTATAT 
GGCGTCAAAC GGGAGCTTGA ACCAGCTGGA GCCGGAGGTG TGGTTGTCCC TGAAGAAGGT 
ACAGCCGATT GGGAAGATCC TCGCGCAACT CCTTCCCCTC CTTCAGCAAG GGTTCTACCT 
GCCAGAACGG CCAAACCAGC AGACATGTCT CGAACTGATC CTGTAGTGCT TGTGTTCGTT 
GAGAGCTTGT ATTCCCAGTT GGGACAGGAC ATTGTTGCCA TCTTGGAATC TGGAAGATTT 
CGGTATCAGA CAGAGATTGC GCCTGGAAAA GGTGACATGC CAACACTCAC AGATAAGAAC 
CGGGGTCGAT TCACGTTGGT CATCTACGAG AACATCCTCA AGTATGTCAA CCTGGATGCC 
TGGAATAGAG AGTTGCTGGA TAAGTACTGT GTGGAGTACG GCGTTGGCAT CATTGGCTTT 
TTCAAGGCTA ATGAAAACAG TTTGCAGAGC GCCCAGCTTA AGGGCTTTCC TCTCTTCCTG 
CACTCCAATC TGGGTTTAAG GGACTGTAGT GTCAACCCAA AGTCTCCACT GCTGTTCATC 
ACCAAGGCCA GAGAAGTGGA GCATGGACCC TTACCTGGAG ATGACTGGAC TGTCTTCCAG 
TCCAACCATA CCACCTATGA GCCTGTGCTG TTAGCTCGGG GACGGAGTAC GGAGGCCAGC 
GGAGCTCCCG GACCCCTTCA TGCAGCTGTG GTTCAGGATT TGGGTCTTCA TGATGGCATC 
CAGCGGGTAC TCTTCGGCAA CAACCTCAAC TTTTGGCTGC ACAAACTGGT ATTGGTGGAC 
GCAGTGAGTT TCTTGACTGG AAAGCGCCTC TCGTTGGCCC TTGAGCGCTA CATACTGGTG 
GATATTGATG ACATCTTTGT TGGGAAGGAG GGAACACGGA TGAAGGTGTC TGATGTCAAG 
GCTCTACTGG AGACGCAGAA TGAACTTCGA AACCATGTGC CCAATTTCAC CTTCAACTTG 
GGATTCTCTG GAAAGTTCTT TCATGCGGGC ACAGATGAGG AGGATCTTGG AGATGACCTG 
CTTCTGTCTT ATAGTAAGGA GTTCTGGTGG TTCCCACACA TGTGGAGTCA CATGCAGCCG 
CATCTATTTC ACAACCAGTC GGTGCTGGCT GAACAAATGC TGCTCAACCA CAAGTTTGCA 
GAGGAGCATG GCATTCCTAC AAACATGGGC TATGCAGTGG CTCCCCATCA CTCTGGAGTG 
TATCCTATCC ATCTGCAGCT GTACGAGGCC TGGAAGAAAG TGTGGGGCAT CAAAGTGACC 
AGTACAGAGG AGTATCCGCA CCTCAAACCT GCACGCTTCC GCAGGGGCTT TGTACACAGT 
GGCATTAGTG TTTTACCCAG ACAGACTTGT GGTCTCTTTA CTCACACCAT CTTCTACAAT 
GAATATCCTG GAGGCTCTAA AGAATTGGAC AAACTCATAT ATGGAGGAGA GCTTTTTCTC 
ACTGTGCTAC TTAACCCAAT CAGCATCTTC ATGACCCACC TGTCCAACTA CGGAAATGAC 
CGCCTTGGCT TGTACACCTT TAAGAACCTG GTGAAGTTCG TCCAGATGTG GACCAATCTA 
AGCCTGCAGA CTCTCCCACC TGTTCAGCTG GCGCAGAAAT ACTTCCAGAT CTTCCCCGAA 
GAGAGAGACC CCATCTGGCA GGACCCCTGT GAGGACAAAA GGCATAAAGA CATCTGGTCT 
AAGGAAAAGA CCTGTGACCG TTTCCCCAAG CTGCTTATCA TTGGACCTCA GAAGACTGGA 
ACCACTGCCC TCTATCTCTT TCTGAGCATG CATTCAGATT TGACCAGTAA CTACCCCAGC 
AAAGAGACCT TCGAGGAAAT CCAGTTCTTC AACGGCCGCA ACTACCACAA AGGAATTGAC 
TGGTACATGG AACATTTCCC TCTACCTTCC AACACTAGCT CAGACTTCTA CTTTGAGAAA 
AGTGCCAACT ACTTTGACTC AGAGGTCGCA GCCCGGCGTG CAGCTGCTCT TCTGCCTAAA 
GCCAAAATCA TCACCATCCT TATCAACCCG GCTGACAGAG CCTATTCCTG GTATCAGCAC 
CAGAGAGCTC ACGATGATCC TGTGGCTCAG AAATATACAT TCCATGATGT CATCACTGCA 
GGCCGGGACG CGCCCATTAA ACTGAGGGTC CTGCAGAGCC GGTGTCTGGT GCCCGGGCTT 
TATGCCACAC ACCTACAGCG GTGGCTCACA CACTATCACC CGAGTCAGAT ATTGGTCCTA 
GATGGTCAGA TGCTGAGGAC AGAACCAGCT TCAGTAATGG ACAAAATCCA GAAGTTTTTA 
GGCCTAATAA ATACACTCAA CTACCACAAG ATACTTGCGT TCGATCCGAA GAAGGGCTTC 
TGGTGTCAGC TGCTGGATGG AGGCAAGACC AAATGCTTGG GGAAGAGTAA AGGGAGGAGA 
TACCCAGACA TGGATGTTGA TTCCCGAACC TTTCTGAGAG AGTATTACCA TGAGCACAAC 
ATTGAACTCT CCAAGCTGCT GTACAAAATG GGTCAGCCGT TGCCCAGCTG GCTAAGAGAG 
GAGCTACTCC ACAGCAGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293866.1
CDS	1009..3669
Translation	MMLMCGRVRRLFRQLTLQTSLLLLFLFCMVSVLISAYFLYGVKRELEPAGAGGVVVPEEGTADWEDPRATPSPPSARVLPARTAKPADMSRTDPVVLVFVESLYSQLGQDIVAILESGRFRYQTEIAPGKGDMPTLTDKNRGRFTLVIYENILKYVNLDAWNRELLDKYCVEYGVGIIGFFKANENSLQSAQLKGFPLFLHSNLGLRDCSVNPKSPLLFITKAREVEHGPLPGDDWTVFQSNHTTYEPVLLARGRSTEASGAPGPLHAAVVQDLGLHDGIQRVLFGNNLNFWLHKLVLVDAVSFLTGKRLSLALERYILVDIDDIFVGKEGTRMKVSDVKALLETQNELRNHVPNFTFNLGFSGKFFHAGTDEEDLGDDLLLSYSKEFWWFPHMWSHMQPHLFHNQSVLAEQMLLNHKFAEEHGIPTNMGYAVAPHHSGVYPIHLQLYEAWKKVWGIKVTSTEEYPHLKPARFRRGFVHSGISVLPRQTCGLFTHTIFYNEYPGGSKELDKLIYGGELFLTVLLNPISIFMTHLSNYGNDRLGLYTFKNLVKFVQMWTNLSLQTLPPVQLAQKYFQIFPEERDPIWQDPCEDKRHKDIWSKEKTCDRFPKLLIIGPQKTGTTALYLFLSMHSDLTSNYPSKETFEEIQFFNGRNYHKGIDWYMEHFPLPSNTSSDFYFEKSANYFDSEVAARRAAALLPKAKIITILINPADRAYSWYQHQRAHDDPVAQKYTFHDVITAGRDAPIKLRVLQSRCLVPGLYATHLQRWLTHYHPSQILVLDGQMLRTEPASVMDKIQKFLGLINTLNYHKILAFDPKKGFWCQLLDGGKTKCLGKSKGRRYPDMDVDSRTFLREYYHEHNIELSKLLYKMGQPLPSWLREELLHSR

Target ORF information:

RefSeq Version	XM_009295591.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1b (ndst1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295591.2

ORF Insert Sequence:

ATGATGCTGA TGTGTGGACG TGTAAGGCGG CTCTTCCGTC AGCTCACGCT GCAGACCAGC 
CTGCTGCTGC TCTTTCTCTT TTGTATGGTC AGTGTCCTCA TCTCAGCCTA TTTCCTATAT 
GGCGTCAAAC GGGAGCTTGA ACCAGCTGGA GCCGGAGGTG TGGTTGTCCC TGAAGAAGGT 
ACAGCCGATT GGGAAGATCC TCGCGCAACT CCTTCCCCTC CTTCAGCAAG GGTTCTACCT 
GCCAGAACGG CCAAACCAGC AGACATGTCT CGAACTGATC CTGTAGTGCT TGTGTTCGTT 
GAGAGCTTGT ATTCCCAGTT GGGACAGGAC ATTGTTGCCA TCTTGGAATC TGGAAGATTT 
CGGTATCAGA CAGAGATTGC GCCTGGAAAA GGTGACATGC CAACACTCAC AGATAAGAAC 
CGGGGTCGAT TCACGTTGGT CATCTACGAG AACATCCTCA AGTATGTCAA CCTGGATGCC 
TGGAATAGAG AGTTGCTGGA TAAGTACTGT GTGGAGTACG GCGTTGGCAT CATTGGCTTT 
TTCAAGGCTA ATGAAAACAG TTTGCAGAGC GCCCAGCTTA AGGGCTTTCC TCTCTTCCTG 
CACTCCAATC TGGGTTTAAG GGACTGTAGT GTCAACCCAA AGTCTCCACT GCTGTTCATC 
ACCAAGGCCA GAGAAGTGGA GCATGGACCC TTACCTGGAG ATGACTGGAC TGTCTTCCAG 
TCCAACCATA CCACCTATGA GCCTGTGCTG TTAGCTCGGG GACGGAGTAC GGAGGCCAGC 
GGAGCTCCCG GACCCCTTCA TGCAGCTGTG GTTCAGGATT TGGGTCTTCA TGATGGCATC 
CAGCGGGTAC TCTTCGGCAA CAACCTCAAC TTTTGGCTGC ACAAACTGGT ATTGGTGGAC 
GCAGTGAGTT TCTTGACTGG AAAGCGCCTC TCGTTGGCCC TTGAGCGCTA CATACTGGTG 
GATATTGATG ACATCTTTGT TGGGAAGGAG GGAACACGGA TGAAGGTGTC TGATGTCAAG 
GCTCTACTGG AGACGCAGAA TGAACTTCGA AACCATGTGC CCAATTTCAC CTTCAACTTG 
GGATTCTCTG GAAAGTTCTT TCATGCGGGC ACAGATGAGG AGGATCTTGG AGATGACCTG 
CTTCTGTCTT ATAGTAAGGA GTTCTGGTGG TTCCCACACA TGTGGAGTCA CATGCAGCCG 
CATCTATTTC ACAACCAGTC GGTGCTGGCT GAACAAATGC TGCTCAACCA CAAGTTTGCA 
GAGGAGCATG GCATTCCTAC AAACATGGGC TATGCAGTGG CTCCCCATCA CTCTGGAGTG 
TATCCTATCC ATCTGCAGCT GTACGAGGCC TGGAAGAAAG TGTGGGGCAT CAAAGTGACC 
AGTACAGAGG AGTATCCGCA CCTCAAACCT GCACGCTTCC GCAGGGGCTT TGTACACAGT 
GGCATTAGTG TTTTACCCAG ACAGACTTGT GGTCTCTTTA CTCACACCAT CTTCTACAAT 
GAATATCCTG GAGGCTCTAA AGAATTGGAC AAACTCATAT ATGGAGGAGA GCTTTTTCTC 
ACTGTGCTAC TTAACCCAAT CAGCATCTTC ATGACCCACC TGTCCAACTA CGGAAATGAC 
CGCCTTGGCT TGTACACCTT TAAGAACCTG GTGAAGTTCG TCCAGATGTG GACCAATCTA 
AGCCTGCAGA CTCTCCCACC TGTTCAGCTG GCGCAGAAAT ACTTCCAGAT CTTCCCCGAA 
GAGAGAGACC CCATCTGGCA GGACCCCTGT GAGGACAAAA GGCATAAAGA CATCTGGTCT 
AAGGAAAAGA CCTGTGACCG TTTCCCCAAG CTGCTTATCA TTGGACCTCA GAAGACTGGA 
ACCACTGCCC TCTATCTCTT TCTGAGCATG CATTCAGATT TGACCAGTAA CTACCCCAGC 
AAAGAGACCT TCGAGGAAAT CCAGTTCTTC AACGGCCGCA ACTACCACAA AGGAATTGAC 
TGGTACATGG AACATTTCCC TCTACCTTCC AACACTAGCT CAGACTTCTA CTTTGAGAAA 
AGTGCCAACT ACTTTGACTC AGAGGTCGCA GCCCGGCGTG CAGCTGCTCT TCTGCCTAAA 
GCCAAAATCA TCACCATCCT TATCAACCCG GCTGACAGAG CCTATTCCTG GTATCAGCAC 
CAGAGAGCTC ACGATGATCC TGTGGCTCAG AAATATACAT TCCATGATGT CATCACTGCA 
GGCCGGGACG CGCCCATTAA ACTGAGGGTC CTGCAGAGCC GGTGTCTGGT GCCCGGGCTT 
TATGCCACAC ACCTACAGCG GTGGCTCACA CACTATCACC CGAGTCAGAT ATTGGTCCTA 
GATGGTCAGA TGCTGAGGAC AGAACCAGCT TCAGTAATGG ACAAAATCCA GAAGTTTTTA 
GGCCTAATAA ATACACTCAA CTACCACAAG ATACTTGCGT TCGATCCGAA GAAGGGCTTC 
TGGTGTCAGC TGCTGGATGG AGGCAAGACC AAATGCTTGG GGAAGAGTAA AGGGAGGAGA 
TACCCAGACA TGGATGTTGA TTCCCGAACC TTTCTGAGAG AGTATTACCA TGAGCACAAC 
ATTGAACTCT CCAAGCTGCT GTACAAAATG GGTCAGCCGT TGCCCAGCTG GCTAAGAGAG 
GAGCTACTCC ACAGCAGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ndst1b ( XM_009295591.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293866.1 Danio rerio N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1b (ndst1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa45974
Clone ID Related Accession (Same CDS sequence)	NM_001324455.1 , XM_009295591.2 , XM_009295591.3 , XM_017352818.1
Accession Version	XM_009295591.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295591.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTGA TGTGTGGACG TGTAAGGCGG CTCTTCCGTC AGCTCACGCT GCAGACCAGC 
CTGCTGCTGC TCTTTCTCTT TTGTATGGTC AGTGTCCTCA TCTCAGCCTA TTTCCTATAT 
GGCGTCAAAC GGGAGCTTGA ACCAGCTGGA GCCGGAGGTG TGGTTGTCCC TGAAGAAGGT 
ACAGCCGATT GGGAAGATCC TCGCGCAACT CCTTCCCCTC CTTCAGCAAG GGTTCTACCT 
GCCAGAACGG CCAAACCAGC AGACATGTCT CGAACTGATC CTGTAGTGCT TGTGTTCGTT 
GAGAGCTTGT ATTCCCAGTT GGGACAGGAC ATTGTTGCCA TCTTGGAATC TGGAAGATTT 
CGGTATCAGA CAGAGATTGC GCCTGGAAAA GGTGACATGC CAACACTCAC AGATAAGAAC 
CGGGGTCGAT TCACGTTGGT CATCTACGAG AACATCCTCA AGTATGTCAA CCTGGATGCC 
TGGAATAGAG AGTTGCTGGA TAAGTACTGT GTGGAGTACG GCGTTGGCAT CATTGGCTTT 
TTCAAGGCTA ATGAAAACAG TTTGCAGAGC GCCCAGCTTA AGGGCTTTCC TCTCTTCCTG 
CACTCCAATC TGGGTTTAAG GGACTGTAGT GTCAACCCAA AGTCTCCACT GCTGTTCATC 
ACCAAGGCCA GAGAAGTGGA GCATGGACCC TTACCTGGAG ATGACTGGAC TGTCTTCCAG 
TCCAACCATA CCACCTATGA GCCTGTGCTG TTAGCTCGGG GACGGAGTAC GGAGGCCAGC 
GGAGCTCCCG GACCCCTTCA TGCAGCTGTG GTTCAGGATT TGGGTCTTCA TGATGGCATC 
CAGCGGGTAC TCTTCGGCAA CAACCTCAAC TTTTGGCTGC ACAAACTGGT ATTGGTGGAC 
GCAGTGAGTT TCTTGACTGG AAAGCGCCTC TCGTTGGCCC TTGAGCGCTA CATACTGGTG 
GATATTGATG ACATCTTTGT TGGGAAGGAG GGAACACGGA TGAAGGTGTC TGATGTCAAG 
GCTCTACTGG AGACGCAGAA TGAACTTCGA AACCATGTGC CCAATTTCAC CTTCAACTTG 
GGATTCTCTG GAAAGTTCTT TCATGCGGGC ACAGATGAGG AGGATCTTGG AGATGACCTG 
CTTCTGTCTT ATAGTAAGGA GTTCTGGTGG TTCCCACACA TGTGGAGTCA CATGCAGCCG 
CATCTATTTC ACAACCAGTC GGTGCTGGCT GAACAAATGC TGCTCAACCA CAAGTTTGCA 
GAGGAGCATG GCATTCCTAC AAACATGGGC TATGCAGTGG CTCCCCATCA CTCTGGAGTG 
TATCCTATCC ATCTGCAGCT GTACGAGGCC TGGAAGAAAG TGTGGGGCAT CAAAGTGACC 
AGTACAGAGG AGTATCCGCA CCTCAAACCT GCACGCTTCC GCAGGGGCTT TGTACACAGT 
GGCATTAGTG TTTTACCCAG ACAGACTTGT GGTCTCTTTA CTCACACCAT CTTCTACAAT 
GAATATCCTG GAGGCTCTAA AGAATTGGAC AAACTCATAT ATGGAGGAGA GCTTTTTCTC 
ACTGTGCTAC TTAACCCAAT CAGCATCTTC ATGACCCACC TGTCCAACTA CGGAAATGAC 
CGCCTTGGCT TGTACACCTT TAAGAACCTG GTGAAGTTCG TCCAGATGTG GACCAATCTA 
AGCCTGCAGA CTCTCCCACC TGTTCAGCTG GCGCAGAAAT ACTTCCAGAT CTTCCCCGAA 
GAGAGAGACC CCATCTGGCA GGACCCCTGT GAGGACAAAA GGCATAAAGA CATCTGGTCT 
AAGGAAAAGA CCTGTGACCG TTTCCCCAAG CTGCTTATCA TTGGACCTCA GAAGACTGGA 
ACCACTGCCC TCTATCTCTT TCTGAGCATG CATTCAGATT TGACCAGTAA CTACCCCAGC 
AAAGAGACCT TCGAGGAAAT CCAGTTCTTC AACGGCCGCA ACTACCACAA AGGAATTGAC 
TGGTACATGG AACATTTCCC TCTACCTTCC AACACTAGCT CAGACTTCTA CTTTGAGAAA 
AGTGCCAACT ACTTTGACTC AGAGGTCGCA GCCCGGCGTG CAGCTGCTCT TCTGCCTAAA 
GCCAAAATCA TCACCATCCT TATCAACCCG GCTGACAGAG CCTATTCCTG GTATCAGCAC 
CAGAGAGCTC ACGATGATCC TGTGGCTCAG AAATATACAT TCCATGATGT CATCACTGCA 
GGCCGGGACG CGCCCATTAA ACTGAGGGTC CTGCAGAGCC GGTGTCTGGT GCCCGGGCTT 
TATGCCACAC ACCTACAGCG GTGGCTCACA CACTATCACC CGAGTCAGAT ATTGGTCCTA 
GATGGTCAGA TGCTGAGGAC AGAACCAGCT TCAGTAATGG ACAAAATCCA GAAGTTTTTA 
GGCCTAATAA ATACACTCAA CTACCACAAG ATACTTGCGT TCGATCCGAA GAAGGGCTTC 
TGGTGTCAGC TGCTGGATGG AGGCAAGACC AAATGCTTGG GGAAGAGTAA AGGGAGGAGA 
TACCCAGACA TGGATGTTGA TTCCCGAACC TTTCTGAGAG AGTATTACCA TGAGCACAAC 
ATTGAACTCT CCAAGCTGCT GTACAAAATG GGTCAGCCGT TGCCCAGCTG GCTAAGAGAG 
GAGCTACTCC ACAGCAGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293866.1
CDS	1017..3677
Translation	MMLMCGRVRRLFRQLTLQTSLLLLFLFCMVSVLISAYFLYGVKRELEPAGAGGVVVPEEGTADWEDPRATPSPPSARVLPARTAKPADMSRTDPVVLVFVESLYSQLGQDIVAILESGRFRYQTEIAPGKGDMPTLTDKNRGRFTLVIYENILKYVNLDAWNRELLDKYCVEYGVGIIGFFKANENSLQSAQLKGFPLFLHSNLGLRDCSVNPKSPLLFITKAREVEHGPLPGDDWTVFQSNHTTYEPVLLARGRSTEASGAPGPLHAAVVQDLGLHDGIQRVLFGNNLNFWLHKLVLVDAVSFLTGKRLSLALERYILVDIDDIFVGKEGTRMKVSDVKALLETQNELRNHVPNFTFNLGFSGKFFHAGTDEEDLGDDLLLSYSKEFWWFPHMWSHMQPHLFHNQSVLAEQMLLNHKFAEEHGIPTNMGYAVAPHHSGVYPIHLQLYEAWKKVWGIKVTSTEEYPHLKPARFRRGFVHSGISVLPRQTCGLFTHTIFYNEYPGGSKELDKLIYGGELFLTVLLNPISIFMTHLSNYGNDRLGLYTFKNLVKFVQMWTNLSLQTLPPVQLAQKYFQIFPEERDPIWQDPCEDKRHKDIWSKEKTCDRFPKLLIIGPQKTGTTALYLFLSMHSDLTSNYPSKETFEEIQFFNGRNYHKGIDWYMEHFPLPSNTSSDFYFEKSANYFDSEVAARRAAALLPKAKIITILINPADRAYSWYQHQRAHDDPVAQKYTFHDVITAGRDAPIKLRVLQSRCLVPGLYATHLQRWLTHYHPSQILVLDGQMLRTEPASVMDKIQKFLGLINTLNYHKILAFDPKKGFWCQLLDGGKTKCLGKSKGRRYPDMDVDSRTFLREYYHEHNIELSKLLYKMGQPLPSWLREELLHSR

Target ORF information:

RefSeq Version	XM_009295591.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1b (ndst1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295591.3

ORF Insert Sequence:

ATGATGCTGA TGTGTGGACG TGTAAGGCGG CTCTTCCGTC AGCTCACGCT GCAGACCAGC 
CTGCTGCTGC TCTTTCTCTT TTGTATGGTC AGTGTCCTCA TCTCAGCCTA TTTCCTATAT 
GGCGTCAAAC GGGAGCTTGA ACCAGCTGGA GCCGGAGGTG TGGTTGTCCC TGAAGAAGGT 
ACAGCCGATT GGGAAGATCC TCGCGCAACT CCTTCCCCTC CTTCAGCAAG GGTTCTACCT 
GCCAGAACGG CCAAACCAGC AGACATGTCT CGAACTGATC CTGTAGTGCT TGTGTTCGTT 
GAGAGCTTGT ATTCCCAGTT GGGACAGGAC ATTGTTGCCA TCTTGGAATC TGGAAGATTT 
CGGTATCAGA CAGAGATTGC GCCTGGAAAA GGTGACATGC CAACACTCAC AGATAAGAAC 
CGGGGTCGAT TCACGTTGGT CATCTACGAG AACATCCTCA AGTATGTCAA CCTGGATGCC 
TGGAATAGAG AGTTGCTGGA TAAGTACTGT GTGGAGTACG GCGTTGGCAT CATTGGCTTT 
TTCAAGGCTA ATGAAAACAG TTTGCAGAGC GCCCAGCTTA AGGGCTTTCC TCTCTTCCTG 
CACTCCAATC TGGGTTTAAG GGACTGTAGT GTCAACCCAA AGTCTCCACT GCTGTTCATC 
ACCAAGGCCA GAGAAGTGGA GCATGGACCC TTACCTGGAG ATGACTGGAC TGTCTTCCAG 
TCCAACCATA CCACCTATGA GCCTGTGCTG TTAGCTCGGG GACGGAGTAC GGAGGCCAGC 
GGAGCTCCCG GACCCCTTCA TGCAGCTGTG GTTCAGGATT TGGGTCTTCA TGATGGCATC 
CAGCGGGTAC TCTTCGGCAA CAACCTCAAC TTTTGGCTGC ACAAACTGGT ATTGGTGGAC 
GCAGTGAGTT TCTTGACTGG AAAGCGCCTC TCGTTGGCCC TTGAGCGCTA CATACTGGTG 
GATATTGATG ACATCTTTGT TGGGAAGGAG GGAACACGGA TGAAGGTGTC TGATGTCAAG 
GCTCTACTGG AGACGCAGAA TGAACTTCGA AACCATGTGC CCAATTTCAC CTTCAACTTG 
GGATTCTCTG GAAAGTTCTT TCATGCGGGC ACAGATGAGG AGGATCTTGG AGATGACCTG 
CTTCTGTCTT ATAGTAAGGA GTTCTGGTGG TTCCCACACA TGTGGAGTCA CATGCAGCCG 
CATCTATTTC ACAACCAGTC GGTGCTGGCT GAACAAATGC TGCTCAACCA CAAGTTTGCA 
GAGGAGCATG GCATTCCTAC AAACATGGGC TATGCAGTGG CTCCCCATCA CTCTGGAGTG 
TATCCTATCC ATCTGCAGCT GTACGAGGCC TGGAAGAAAG TGTGGGGCAT CAAAGTGACC 
AGTACAGAGG AGTATCCGCA CCTCAAACCT GCACGCTTCC GCAGGGGCTT TGTACACAGT 
GGCATTAGTG TTTTACCCAG ACAGACTTGT GGTCTCTTTA CTCACACCAT CTTCTACAAT 
GAATATCCTG GAGGCTCTAA AGAATTGGAC AAACTCATAT ATGGAGGAGA GCTTTTTCTC 
ACTGTGCTAC TTAACCCAAT CAGCATCTTC ATGACCCACC TGTCCAACTA CGGAAATGAC 
CGCCTTGGCT TGTACACCTT TAAGAACCTG GTGAAGTTCG TCCAGATGTG GACCAATCTA 
AGCCTGCAGA CTCTCCCACC TGTTCAGCTG GCGCAGAAAT ACTTCCAGAT CTTCCCCGAA 
GAGAGAGACC CCATCTGGCA GGACCCCTGT GAGGACAAAA GGCATAAAGA CATCTGGTCT 
AAGGAAAAGA CCTGTGACCG TTTCCCCAAG CTGCTTATCA TTGGACCTCA GAAGACTGGA 
ACCACTGCCC TCTATCTCTT TCTGAGCATG CATTCAGATT TGACCAGTAA CTACCCCAGC 
AAAGAGACCT TCGAGGAAAT CCAGTTCTTC AACGGCCGCA ACTACCACAA AGGAATTGAC 
TGGTACATGG AACATTTCCC TCTACCTTCC AACACTAGCT CAGACTTCTA CTTTGAGAAA 
AGTGCCAACT ACTTTGACTC AGAGGTCGCA GCCCGGCGTG CAGCTGCTCT TCTGCCTAAA 
GCCAAAATCA TCACCATCCT TATCAACCCG GCTGACAGAG CCTATTCCTG GTATCAGCAC 
CAGAGAGCTC ACGATGATCC TGTGGCTCAG AAATATACAT TCCATGATGT CATCACTGCA 
GGCCGGGACG CGCCCATTAA ACTGAGGGTC CTGCAGAGCC GGTGTCTGGT GCCCGGGCTT 
TATGCCACAC ACCTACAGCG GTGGCTCACA CACTATCACC CGAGTCAGAT ATTGGTCCTA 
GATGGTCAGA TGCTGAGGAC AGAACCAGCT TCAGTAATGG ACAAAATCCA GAAGTTTTTA 
GGCCTAATAA ATACACTCAA CTACCACAAG ATACTTGCGT TCGATCCGAA GAAGGGCTTC 
TGGTGTCAGC TGCTGGATGG AGGCAAGACC AAATGCTTGG GGAAGAGTAA AGGGAGGAGA 
TACCCAGACA TGGATGTTGA TTCCCGAACC TTTCTGAGAG AGTATTACCA TGAGCACAAC 
ATTGAACTCT CCAAGCTGCT GTACAAAATG GGTCAGCCGT TGCCCAGCTG GCTAAGAGAG 
GAGCTACTCC ACAGCAGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ndst1b ( XM_017352818.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208307.1 Danio rerio N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1b (ndst1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa45974
Clone ID Related Accession (Same CDS sequence)	NM_001324455.1 , XM_009295591.2 , XM_009295591.3 , XM_017352818.1
Accession Version	XM_017352818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTGA TGTGTGGACG TGTAAGGCGG CTCTTCCGTC AGCTCACGCT GCAGACCAGC 
CTGCTGCTGC TCTTTCTCTT TTGTATGGTC AGTGTCCTCA TCTCAGCCTA TTTCCTATAT 
GGCGTCAAAC GGGAGCTTGA ACCAGCTGGA GCCGGAGGTG TGGTTGTCCC TGAAGAAGGT 
ACAGCCGATT GGGAAGATCC TCGCGCAACT CCTTCCCCTC CTTCAGCAAG GGTTCTACCT 
GCCAGAACGG CCAAACCAGC AGACATGTCT CGAACTGATC CTGTAGTGCT TGTGTTCGTT 
GAGAGCTTGT ATTCCCAGTT GGGACAGGAC ATTGTTGCCA TCTTGGAATC TGGAAGATTT 
CGGTATCAGA CAGAGATTGC GCCTGGAAAA GGTGACATGC CAACACTCAC AGATAAGAAC 
CGGGGTCGAT TCACGTTGGT CATCTACGAG AACATCCTCA AGTATGTCAA CCTGGATGCC 
TGGAATAGAG AGTTGCTGGA TAAGTACTGT GTGGAGTACG GCGTTGGCAT CATTGGCTTT 
TTCAAGGCTA ATGAAAACAG TTTGCAGAGC GCCCAGCTTA AGGGCTTTCC TCTCTTCCTG 
CACTCCAATC TGGGTTTAAG GGACTGTAGT GTCAACCCAA AGTCTCCACT GCTGTTCATC 
ACCAAGGCCA GAGAAGTGGA GCATGGACCC TTACCTGGAG ATGACTGGAC TGTCTTCCAG 
TCCAACCATA CCACCTATGA GCCTGTGCTG TTAGCTCGGG GACGGAGTAC GGAGGCCAGC 
GGAGCTCCCG GACCCCTTCA TGCAGCTGTG GTTCAGGATT TGGGTCTTCA TGATGGCATC 
CAGCGGGTAC TCTTCGGCAA CAACCTCAAC TTTTGGCTGC ACAAACTGGT ATTGGTGGAC 
GCAGTGAGTT TCTTGACTGG AAAGCGCCTC TCGTTGGCCC TTGAGCGCTA CATACTGGTG 
GATATTGATG ACATCTTTGT TGGGAAGGAG GGAACACGGA TGAAGGTGTC TGATGTCAAG 
GCTCTACTGG AGACGCAGAA TGAACTTCGA AACCATGTGC CCAATTTCAC CTTCAACTTG 
GGATTCTCTG GAAAGTTCTT TCATGCGGGC ACAGATGAGG AGGATCTTGG AGATGACCTG 
CTTCTGTCTT ATAGTAAGGA GTTCTGGTGG TTCCCACACA TGTGGAGTCA CATGCAGCCG 
CATCTATTTC ACAACCAGTC GGTGCTGGCT GAACAAATGC TGCTCAACCA CAAGTTTGCA 
GAGGAGCATG GCATTCCTAC AAACATGGGC TATGCAGTGG CTCCCCATCA CTCTGGAGTG 
TATCCTATCC ATCTGCAGCT GTACGAGGCC TGGAAGAAAG TGTGGGGCAT CAAAGTGACC 
AGTACAGAGG AGTATCCGCA CCTCAAACCT GCACGCTTCC GCAGGGGCTT TGTACACAGT 
GGCATTAGTG TTTTACCCAG ACAGACTTGT GGTCTCTTTA CTCACACCAT CTTCTACAAT 
GAATATCCTG GAGGCTCTAA AGAATTGGAC AAACTCATAT ATGGAGGAGA GCTTTTTCTC 
ACTGTGCTAC TTAACCCAAT CAGCATCTTC ATGACCCACC TGTCCAACTA CGGAAATGAC 
CGCCTTGGCT TGTACACCTT TAAGAACCTG GTGAAGTTCG TCCAGATGTG GACCAATCTA 
AGCCTGCAGA CTCTCCCACC TGTTCAGCTG GCGCAGAAAT ACTTCCAGAT CTTCCCCGAA 
GAGAGAGACC CCATCTGGCA GGACCCCTGT GAGGACAAAA GGCATAAAGA CATCTGGTCT 
AAGGAAAAGA CCTGTGACCG TTTCCCCAAG CTGCTTATCA TTGGACCTCA GAAGACTGGA 
ACCACTGCCC TCTATCTCTT TCTGAGCATG CATTCAGATT TGACCAGTAA CTACCCCAGC 
AAAGAGACCT TCGAGGAAAT CCAGTTCTTC AACGGCCGCA ACTACCACAA AGGAATTGAC 
TGGTACATGG AACATTTCCC TCTACCTTCC AACACTAGCT CAGACTTCTA CTTTGAGAAA 
AGTGCCAACT ACTTTGACTC AGAGGTCGCA GCCCGGCGTG CAGCTGCTCT TCTGCCTAAA 
GCCAAAATCA TCACCATCCT TATCAACCCG GCTGACAGAG CCTATTCCTG GTATCAGCAC 
CAGAGAGCTC ACGATGATCC TGTGGCTCAG AAATATACAT TCCATGATGT CATCACTGCA 
GGCCGGGACG CGCCCATTAA ACTGAGGGTC CTGCAGAGCC GGTGTCTGGT GCCCGGGCTT 
TATGCCACAC ACCTACAGCG GTGGCTCACA CACTATCACC CGAGTCAGAT ATTGGTCCTA 
GATGGTCAGA TGCTGAGGAC AGAACCAGCT TCAGTAATGG ACAAAATCCA GAAGTTTTTA 
GGCCTAATAA ATACACTCAA CTACCACAAG ATACTTGCGT TCGATCCGAA GAAGGGCTTC 
TGGTGTCAGC TGCTGGATGG AGGCAAGACC AAATGCTTGG GGAAGAGTAA AGGGAGGAGA 
TACCCAGACA TGGATGTTGA TTCCCGAACC TTTCTGAGAG AGTATTACCA TGAGCACAAC 
ATTGAACTCT CCAAGCTGCT GTACAAAATG GGTCAGCCGT TGCCCAGCTG GCTAAGAGAG 
GAGCTACTCC ACAGCAGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208307.1
CDS	857..3517
Translation	MMLMCGRVRRLFRQLTLQTSLLLLFLFCMVSVLISAYFLYGVKRELEPAGAGGVVVPEEGTADWEDPRATPSPPSARVLPARTAKPADMSRTDPVVLVFVESLYSQLGQDIVAILESGRFRYQTEIAPGKGDMPTLTDKNRGRFTLVIYENILKYVNLDAWNRELLDKYCVEYGVGIIGFFKANENSLQSAQLKGFPLFLHSNLGLRDCSVNPKSPLLFITKAREVEHGPLPGDDWTVFQSNHTTYEPVLLARGRSTEASGAPGPLHAAVVQDLGLHDGIQRVLFGNNLNFWLHKLVLVDAVSFLTGKRLSLALERYILVDIDDIFVGKEGTRMKVSDVKALLETQNELRNHVPNFTFNLGFSGKFFHAGTDEEDLGDDLLLSYSKEFWWFPHMWSHMQPHLFHNQSVLAEQMLLNHKFAEEHGIPTNMGYAVAPHHSGVYPIHLQLYEAWKKVWGIKVTSTEEYPHLKPARFRRGFVHSGISVLPRQTCGLFTHTIFYNEYPGGSKELDKLIYGGELFLTVLLNPISIFMTHLSNYGNDRLGLYTFKNLVKFVQMWTNLSLQTLPPVQLAQKYFQIFPEERDPIWQDPCEDKRHKDIWSKEKTCDRFPKLLIIGPQKTGTTALYLFLSMHSDLTSNYPSKETFEEIQFFNGRNYHKGIDWYMEHFPLPSNTSSDFYFEKSANYFDSEVAARRAAALLPKAKIITILINPADRAYSWYQHQRAHDDPVAQKYTFHDVITAGRDAPIKLRVLQSRCLVPGLYATHLQRWLTHYHPSQILVLDGQMLRTEPASVMDKIQKFLGLINTLNYHKILAFDPKKGFWCQLLDGGKTKCLGKSKGRRYPDMDVDSRTFLREYYHEHNIELSKLLYKMGQPLPSWLREELLHSR

Target ORF information:

RefSeq Version	XM_017352818.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1b (ndst1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352818.1

ORF Insert Sequence:

ATGATGCTGA TGTGTGGACG TGTAAGGCGG CTCTTCCGTC AGCTCACGCT GCAGACCAGC 
CTGCTGCTGC TCTTTCTCTT TTGTATGGTC AGTGTCCTCA TCTCAGCCTA TTTCCTATAT 
GGCGTCAAAC GGGAGCTTGA ACCAGCTGGA GCCGGAGGTG TGGTTGTCCC TGAAGAAGGT 
ACAGCCGATT GGGAAGATCC TCGCGCAACT CCTTCCCCTC CTTCAGCAAG GGTTCTACCT 
GCCAGAACGG CCAAACCAGC AGACATGTCT CGAACTGATC CTGTAGTGCT TGTGTTCGTT 
GAGAGCTTGT ATTCCCAGTT GGGACAGGAC ATTGTTGCCA TCTTGGAATC TGGAAGATTT 
CGGTATCAGA CAGAGATTGC GCCTGGAAAA GGTGACATGC CAACACTCAC AGATAAGAAC 
CGGGGTCGAT TCACGTTGGT CATCTACGAG AACATCCTCA AGTATGTCAA CCTGGATGCC 
TGGAATAGAG AGTTGCTGGA TAAGTACTGT GTGGAGTACG GCGTTGGCAT CATTGGCTTT 
TTCAAGGCTA ATGAAAACAG TTTGCAGAGC GCCCAGCTTA AGGGCTTTCC TCTCTTCCTG 
CACTCCAATC TGGGTTTAAG GGACTGTAGT GTCAACCCAA AGTCTCCACT GCTGTTCATC 
ACCAAGGCCA GAGAAGTGGA GCATGGACCC TTACCTGGAG ATGACTGGAC TGTCTTCCAG 
TCCAACCATA CCACCTATGA GCCTGTGCTG TTAGCTCGGG GACGGAGTAC GGAGGCCAGC 
GGAGCTCCCG GACCCCTTCA TGCAGCTGTG GTTCAGGATT TGGGTCTTCA TGATGGCATC 
CAGCGGGTAC TCTTCGGCAA CAACCTCAAC TTTTGGCTGC ACAAACTGGT ATTGGTGGAC 
GCAGTGAGTT TCTTGACTGG AAAGCGCCTC TCGTTGGCCC TTGAGCGCTA CATACTGGTG 
GATATTGATG ACATCTTTGT TGGGAAGGAG GGAACACGGA TGAAGGTGTC TGATGTCAAG 
GCTCTACTGG AGACGCAGAA TGAACTTCGA AACCATGTGC CCAATTTCAC CTTCAACTTG 
GGATTCTCTG GAAAGTTCTT TCATGCGGGC ACAGATGAGG AGGATCTTGG AGATGACCTG 
CTTCTGTCTT ATAGTAAGGA GTTCTGGTGG TTCCCACACA TGTGGAGTCA CATGCAGCCG 
CATCTATTTC ACAACCAGTC GGTGCTGGCT GAACAAATGC TGCTCAACCA CAAGTTTGCA 
GAGGAGCATG GCATTCCTAC AAACATGGGC TATGCAGTGG CTCCCCATCA CTCTGGAGTG 
TATCCTATCC ATCTGCAGCT GTACGAGGCC TGGAAGAAAG TGTGGGGCAT CAAAGTGACC 
AGTACAGAGG AGTATCCGCA CCTCAAACCT GCACGCTTCC GCAGGGGCTT TGTACACAGT 
GGCATTAGTG TTTTACCCAG ACAGACTTGT GGTCTCTTTA CTCACACCAT CTTCTACAAT 
GAATATCCTG GAGGCTCTAA AGAATTGGAC AAACTCATAT ATGGAGGAGA GCTTTTTCTC 
ACTGTGCTAC TTAACCCAAT CAGCATCTTC ATGACCCACC TGTCCAACTA CGGAAATGAC 
CGCCTTGGCT TGTACACCTT TAAGAACCTG GTGAAGTTCG TCCAGATGTG GACCAATCTA 
AGCCTGCAGA CTCTCCCACC TGTTCAGCTG GCGCAGAAAT ACTTCCAGAT CTTCCCCGAA 
GAGAGAGACC CCATCTGGCA GGACCCCTGT GAGGACAAAA GGCATAAAGA CATCTGGTCT 
AAGGAAAAGA CCTGTGACCG TTTCCCCAAG CTGCTTATCA TTGGACCTCA GAAGACTGGA 
ACCACTGCCC TCTATCTCTT TCTGAGCATG CATTCAGATT TGACCAGTAA CTACCCCAGC 
AAAGAGACCT TCGAGGAAAT CCAGTTCTTC AACGGCCGCA ACTACCACAA AGGAATTGAC 
TGGTACATGG AACATTTCCC TCTACCTTCC AACACTAGCT CAGACTTCTA CTTTGAGAAA 
AGTGCCAACT ACTTTGACTC AGAGGTCGCA GCCCGGCGTG CAGCTGCTCT TCTGCCTAAA 
GCCAAAATCA TCACCATCCT TATCAACCCG GCTGACAGAG CCTATTCCTG GTATCAGCAC 
CAGAGAGCTC ACGATGATCC TGTGGCTCAG AAATATACAT TCCATGATGT CATCACTGCA 
GGCCGGGACG CGCCCATTAA ACTGAGGGTC CTGCAGAGCC GGTGTCTGGT GCCCGGGCTT 
TATGCCACAC ACCTACAGCG GTGGCTCACA CACTATCACC CGAGTCAGAT ATTGGTCCTA 
GATGGTCAGA TGCTGAGGAC AGAACCAGCT TCAGTAATGG ACAAAATCCA GAAGTTTTTA 
GGCCTAATAA ATACACTCAA CTACCACAAG ATACTTGCGT TCGATCCGAA GAAGGGCTTC 
TGGTGTCAGC TGCTGGATGG AGGCAAGACC AAATGCTTGG GGAAGAGTAA AGGGAGGAGA 
TACCCAGACA TGGATGTTGA TTCCCGAACC TTTCTGAGAG AGTATTACCA TGAGCACAAC 
ATTGAACTCT CCAAGCTGCT GTACAAAATG GGTCAGCCGT TGCCCAGCTG GCTAAGAGAG 
GAGCTACTCC ACAGCAGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

The NDST gene family in zebrafish: role of NDST1B in pharyngeal arch formation.
PloS one10(3)e0119040(2015)
Filipek-G?rniok B,Carlsson P,Haitina T,Habicher J,Ledin J,Kjell?n L

Evolution of the miR199-214 cluster and vertebrate skeletal development.
RNA biology11(4)281-94(2014)
Desvignes T,Contreras A,Postlethwait JH

Glycome and transcriptome regulation of vasculogenesis.
Circulation120(19)1883-1892(2009 Nov)
Harfouche R,Hentschel DM,Piecewicz S,Basu S,Print C,Eavarone D,Kiziltepe T,Sasisekharan R,Sengupta S

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ndst1b cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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