fyco1a cDNA ORF clone, Danio rerio(zebrafish)

The following fyco1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fyco1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa57511 XM_017353733.2
Latest version!
Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa44696 XM_009297091.3
Latest version!
Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa44696 XM_009297090.3
Latest version!
Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
ODa57511 XM_017353733.1 Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa44696 XM_009297090.2 Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa44696 XM_009297091.2 Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa57511
    Clone ID Related Accession (Same CDS sequence) XM_017353733.2 , XM_017353733.1
    Accession Version XM_017353733.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3537bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product FYVE and coiled-coil domain-containing protein 1-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017353733.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCACTG GTGCTGCAGT CGGGGACAGT CAGATCCAGA GGATTATCAG AGATCTGCGC 
    GATGCTGTTT CCGAACTGAC AAAGGAGTAT AAAGAGAGCG GTGAGCCCAT TACTGATGAC
    AGCTCCAATC TGCACAAGTT CTCCTACAAG CTGGAGTATC TGCTTCAGTT CGACCAAAAG
    GAGAAAACAA CATTTCTGGG CTCCAGAAAA GACTACTGGG ATTACTTCAG CGACTGCCTG
    GCCAAAATCA AAGGAGCAAA TGATGGAATT CGCTTCGTTA AATCAATTTC AGAGCTGAAG
    ACGTCTCTGG GCAAGGGCAG AGCATTTATC CGTTACTCTC TGGTGCATCA GCGGCTGGCA
    GACACACTCC AGCAGTGCCT CATGAACTCC AGAGTCACCA GTGACTGGTA TAATCCGAGG
    AGTCCGTTTC TCAAACCCCA TCTCAGCGTG GACATCATTA GTTATCTCTA CGAGCTCAAT
    GATGTCCAGT TTGATGTGGC ATCCAGAGGT CATGACCTTG ACGCAGAATG GCCCACTTTT
    GCCAGGAGGA CATTAGGAAT GACTAGCTCT CCCAGTCACT TGTGGAAAGC ACCGAGTCGT
    AGTTCCAGCA TCAACAGTCT GGCCAGCACA TACTCACAGC AGCACCATGA GTTCCCTGGG
    AGTCCAGACT TTGGACCTGG GCTTTTATCA GATATGTCTA TGCAAAACTC CAGCATACTG
    AATGATACCT CAATGAGTGC TATAGACGAA CTCCGTCTGG AGCTTGACCA GTCTGAATTA
    AAGCAAAGAG AGCTTATTGA CCGGATTCAG CAGTTAGGTG ATGAGGGGAG TGAACTGCGT
    GGAGTTGTTG TGGAACTGCA AAGGCAGCTA GATGTTTCTT TAGCGGCCCA AGGTAACCAT
    CAGGAGCTAC AACGTAACCT GGAGGTCTTA ATTGAGAGCG AACATGCACT CTCCCGTGAG
    GTGGAGGTCT TAAGAGATCG AGAAACCAGG AGGGAGGTTT CCCATAAAGA CCTTCAGGAC
    ATGCTGGCAG CAGCGGAGCG TAAAAACGAG GAGCTGATGA CAAGGCTAGA TGGAGTATTG
    GATGAGAAAG GTCAAAGGGC GGCCAGTGAT TTCAACTCAG CCCAGAAGAT TCACGAGCTT
    TTGAATGAAT TAAAAGAGGC TGAGAAGAAG AGGATGGATG CTCTGGCTGA AGGAGAAGAG
    AAGAGGAGGC ATGCTGAACA TCTTGCCGAG GAAGTCAAAG TGAAAGATGA AGCTCTCAAA
    GAGGCTGAGG TGAAAATGGC AGCTTGGATG GAGAAGGGTG AACAACTGCA AACCAGGGCG
    GTGGAGCAGC GTAATTTCAT GGAGAAACTT CAAGGTGCAC TTGCAGTAAG AGAGAAAGAG
    ACCAGCAACC TGCAGAGGCA ACTACGAGAT CTTCAGAACT CTCTGGAGAA CATGGAAAAG
    CAGGCCAATG TGGAGAAGAA GAGGATGCAA GATGATAAAG AAGAGCTTGA GATGAAAATG
    AATGGTCTAG AGGGGCTGTT ACAATCACTT CGGACACAGT TAAAGGTTAA AGAATCTGAT
    CTTCTCTCCA GTACCAAGAG AGTTCATTTC CTGGAGAGGG AATCAGAGAA GCTTAGGAGC
    GAGAATCAGA AGTTGGAGTA TGAGTTGGAG AACAGCACCA AAAAGGAGGC CAAGAAGATT
    GATGAATACA AGGATAGCTG CGCCAAGCTT ATTGAGCAAA ACACTAAGCT TCTGCAGACG
    GTGAATAAGA ATGAAGAGAG CAAGAAAGAG TTGCTTGAAA ACAAATCATC CTTGGAGAGC
    GAGTTGGCGG GATTGAGGGC CTCAGAGAAG CAGTTACGAG CTCAAATCGA TGATGCCAAA
    GTGACTGTGG ATGAACGAGA GCAGCGGCTG AGAGAGGAGA ACAGAAATCT GGATGAGAGT
    CTGCAGAAGG CCAACATGCA GCTGGAGGAA TCCGAGAGCT CAATTCGGCA AAAGGAGCAG
    GAGAACAAGG ACCTCATGGA GGTTCAGGTC ACTCTTAAAT CAGCTCTCGC AGCCATGCAG
    AAAGAGATAC GTGACATAAA CAACCAGATC GGTGAACTTG AGAAGAATCT TGGAGTTGCG
    AGGTGTAACG AAGCAAATCT GAACGCGCAG CTTAAAGATA AGGCCACCCA GCTGGAAGAT
    CGAGAGAAGC TCTGTGAGGA GCTCCAGGGA AGAGTGGAAG AACTGGAAAG TCGGCAGAGG
    GATTTGGAAG TGGAGAAGAC AAAAGCAGAA AGAGCCTTTG TTAAACAAAC AGAGATGATT
    CAGAGTCTTG AAGCACAGAG AAACCTGGCA GAGAAGACAC AACTTGAAAA AAGCACATGT
    CAAGCTAAAG AAACTAAAGA GATGGCCTTA AAATTGACTC TTCTAGAAGA TCAACTGGGA
    TTGAGTGCGA AGGAAGTTTC TAAGCTACAA GAGGAAGTGG TTAATCTCAG GGCTAAACTT
    CACAGTGCTG TGGAGGAGAA GGACAAGACG CAAGCAAAGC TTGAGGTCAC CGAAGCTTCC
    TGTGCTGAAC TGCGGATCTT GACTGAGCAC TTGAAGAAGC AGGCCGAGGA GCAGAATCGG
    CTGCATGTGA GCGAGCTGCT GCAGTCTAGT GAACATGTTG ATAAATTAAC TTCGCAGCTG
    AACCAGGAGA CCTCAGCGCA TGAGAAAACC ACTGCAGCAC TTGCTTCCGC TAAAGAGGAT
    CTAGTGGCTC TCAAGGCCCA GAATGAGCGG ATGGTCCTGG AGAATGCGGA AACCAGAGAG
    AGTCTCCACC GGGTCAACAC TGAAATGGCA GAGTTGGGAA TGACTATTTG CAAGCTCACC
    GCCGAGCGGG AAGAGGCCCG GGAACGTTGG GCCGCTGAAG CTGTACGCAT CCAAGAACTT
    CAGCAGCACG GAGTGAAGGA GACAGAGCGA CTCAATGCCA GCTTGGTGGC TCTGCATCAA
    GAAAACTCCA GTCTGCAGGA AGAGCTCCAG CAAACAGACA AACTGTCAGA AACCATGCTG
    GAGCTAAAGC AGCTGCTGGA CAAAACTGAG GGCGAACGGG ACGCTGCACG GGAAGAGATC
    ACCGCTGTGA AATTCCAGAT GAGCACAGAG AGCATGTCTC TCAAGCACCA AATGAAGAGT
    CTTCAAGAAG AGATTGACGG TTTGAAAGAT CAGCTAGACA CAGAGAGGAA GAAGAAATCA
    GAGCTGGAGG CCAAATTATC CGAACTAGAG GGAGCGAATG TGGAATACTC TCGTCTGATT
    GAGGAGAAGG ACTCGCACAT TACTTACTGT GAGACTTTGC TGCGTGAGAG CGAGTCTGAG
    ACACAGCAAC TGCAGGAAAG AGCATCAAGA TCCAAAGAGG CTCTTAGTGA TGTAGAGAAG
    GAGAGGGAAG AATTGAAGCA GAAATTGGAT CAAGTGTTGA TGGAGACTCA GAACCAGCAT
    CTCCGCATGT CTGCTGAACT GGAGGACCTG GGACAAACTA AAGTCAACCT GGAGGAACGG
    CTCATTGAAC TTATTAGGTC TGTGTTTCAC CTAAAACTGC AGCTATTTTT TCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209222.1
    CDS156..3692
    Translation

    Target ORF information:

    RefSeq Version XM_017353733.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017353733.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCACTG GTGCTGCAGT CGGGGACAGT CAGATCCAGA GGATTATCAG AGATCTGCGC 
    GATGCTGTTT CCGAACTGAC AAAGGAGTAT AAAGAGAGCG GTGAGCCCAT TACTGATGAC
    AGCTCCAATC TGCACAAGTT CTCCTACAAG CTGGAGTATC TGCTTCAGTT CGACCAAAAG
    GAGAAAACAA CATTTCTGGG CTCCAGAAAA GACTACTGGG ATTACTTCAG CGACTGCCTG
    GCCAAAATCA AAGGAGCAAA TGATGGAATT CGCTTCGTTA AATCAATTTC AGAGCTGAAG
    ACGTCTCTGG GCAAGGGCAG AGCATTTATC CGTTACTCTC TGGTGCATCA GCGGCTGGCA
    GACACACTCC AGCAGTGCCT CATGAACTCC AGAGTCACCA GTGACTGGTA TAATCCGAGG
    AGTCCGTTTC TCAAACCCCA TCTCAGCGTG GACATCATTA GTTATCTCTA CGAGCTCAAT
    GATGTCCAGT TTGATGTGGC ATCCAGAGGT CATGACCTTG ACGCAGAATG GCCCACTTTT
    GCCAGGAGGA CATTAGGAAT GACTAGCTCT CCCAGTCACT TGTGGAAAGC ACCGAGTCGT
    AGTTCCAGCA TCAACAGTCT GGCCAGCACA TACTCACAGC AGCACCATGA GTTCCCTGGG
    AGTCCAGACT TTGGACCTGG GCTTTTATCA GATATGTCTA TGCAAAACTC CAGCATACTG
    AATGATACCT CAATGAGTGC TATAGACGAA CTCCGTCTGG AGCTTGACCA GTCTGAATTA
    AAGCAAAGAG AGCTTATTGA CCGGATTCAG CAGTTAGGTG ATGAGGGGAG TGAACTGCGT
    GGAGTTGTTG TGGAACTGCA AAGGCAGCTA GATGTTTCTT TAGCGGCCCA AGGTAACCAT
    CAGGAGCTAC AACGTAACCT GGAGGTCTTA ATTGAGAGCG AACATGCACT CTCCCGTGAG
    GTGGAGGTCT TAAGAGATCG AGAAACCAGG AGGGAGGTTT CCCATAAAGA CCTTCAGGAC
    ATGCTGGCAG CAGCGGAGCG TAAAAACGAG GAGCTGATGA CAAGGCTAGA TGGAGTATTG
    GATGAGAAAG GTCAAAGGGC GGCCAGTGAT TTCAACTCAG CCCAGAAGAT TCACGAGCTT
    TTGAATGAAT TAAAAGAGGC TGAGAAGAAG AGGATGGATG CTCTGGCTGA AGGAGAAGAG
    AAGAGGAGGC ATGCTGAACA TCTTGCCGAG GAAGTCAAAG TGAAAGATGA AGCTCTCAAA
    GAGGCTGAGG TGAAAATGGC AGCTTGGATG GAGAAGGGTG AACAACTGCA AACCAGGGCG
    GTGGAGCAGC GTAATTTCAT GGAGAAACTT CAAGGTGCAC TTGCAGTAAG AGAGAAAGAG
    ACCAGCAACC TGCAGAGGCA ACTACGAGAT CTTCAGAACT CTCTGGAGAA CATGGAAAAG
    CAGGCCAATG TGGAGAAGAA GAGGATGCAA GATGATAAAG AAGAGCTTGA GATGAAAATG
    AATGGTCTAG AGGGGCTGTT ACAATCACTT CGGACACAGT TAAAGGTTAA AGAATCTGAT
    CTTCTCTCCA GTACCAAGAG AGTTCATTTC CTGGAGAGGG AATCAGAGAA GCTTAGGAGC
    GAGAATCAGA AGTTGGAGTA TGAGTTGGAG AACAGCACCA AAAAGGAGGC CAAGAAGATT
    GATGAATACA AGGATAGCTG CGCCAAGCTT ATTGAGCAAA ACACTAAGCT TCTGCAGACG
    GTGAATAAGA ATGAAGAGAG CAAGAAAGAG TTGCTTGAAA ACAAATCATC CTTGGAGAGC
    GAGTTGGCGG GATTGAGGGC CTCAGAGAAG CAGTTACGAG CTCAAATCGA TGATGCCAAA
    GTGACTGTGG ATGAACGAGA GCAGCGGCTG AGAGAGGAGA ACAGAAATCT GGATGAGAGT
    CTGCAGAAGG CCAACATGCA GCTGGAGGAA TCCGAGAGCT CAATTCGGCA AAAGGAGCAG
    GAGAACAAGG ACCTCATGGA GGTTCAGGTC ACTCTTAAAT CAGCTCTCGC AGCCATGCAG
    AAAGAGATAC GTGACATAAA CAACCAGATC GGTGAACTTG AGAAGAATCT TGGAGTTGCG
    AGGTGTAACG AAGCAAATCT GAACGCGCAG CTTAAAGATA AGGCCACCCA GCTGGAAGAT
    CGAGAGAAGC TCTGTGAGGA GCTCCAGGGA AGAGTGGAAG AACTGGAAAG TCGGCAGAGG
    GATTTGGAAG TGGAGAAGAC AAAAGCAGAA AGAGCCTTTG TTAAACAAAC AGAGATGATT
    CAGAGTCTTG AAGCACAGAG AAACCTGGCA GAGAAGACAC AACTTGAAAA AAGCACATGT
    CAAGCTAAAG AAACTAAAGA GATGGCCTTA AAATTGACTC TTCTAGAAGA TCAACTGGGA
    TTGAGTGCGA AGGAAGTTTC TAAGCTACAA GAGGAAGTGG TTAATCTCAG GGCTAAACTT
    CACAGTGCTG TGGAGGAGAA GGACAAGACG CAAGCAAAGC TTGAGGTCAC CGAAGCTTCC
    TGTGCTGAAC TGCGGATCTT GACTGAGCAC TTGAAGAAGC AGGCCGAGGA GCAGAATCGG
    CTGCATGTGA GCGAGCTGCT GCAGTCTAGT GAACATGTTG ATAAATTAAC TTCGCAGCTG
    AACCAGGAGA CCTCAGCGCA TGAGAAAACC ACTGCAGCAC TTGCTTCCGC TAAAGAGGAT
    CTAGTGGCTC TCAAGGCCCA GAATGAGCGG ATGGTCCTGG AGAATGCGGA AACCAGAGAG
    AGTCTCCACC GGGTCAACAC TGAAATGGCA GAGTTGGGAA TGACTATTTG CAAGCTCACC
    GCCGAGCGGG AAGAGGCCCG GGAACGTTGG GCCGCTGAAG CTGTACGCAT CCAAGAACTT
    CAGCAGCACG GAGTGAAGGA GACAGAGCGA CTCAATGCCA GCTTGGTGGC TCTGCATCAA
    GAAAACTCCA GTCTGCAGGA AGAGCTCCAG CAAACAGACA AACTGTCAGA AACCATGCTG
    GAGCTAAAGC AGCTGCTGGA CAAAACTGAG GGCGAACGGG ACGCTGCACG GGAAGAGATC
    ACCGCTGTGA AATTCCAGAT GAGCACAGAG AGCATGTCTC TCAAGCACCA AATGAAGAGT
    CTTCAAGAAG AGATTGACGG TTTGAAAGAT CAGCTAGACA CAGAGAGGAA GAAGAAATCA
    GAGCTGGAGG CCAAATTATC CGAACTAGAG GGAGCGAATG TGGAATACTC TCGTCTGATT
    GAGGAGAAGG ACTCGCACAT TACTTACTGT GAGACTTTGC TGCGTGAGAG CGAGTCTGAG
    ACACAGCAAC TGCAGGAAAG AGCATCAAGA TCCAAAGAGG CTCTTAGTGA TGTAGAGAAG
    GAGAGGGAAG AATTGAAGCA GAAATTGGAT CAAGTGTTGA TGGAGACTCA GAACCAGCAT
    CTCCGCATGT CTGCTGAACT GGAGGACCTG GGACAAACTA AAGTCAACCT GGAGGAACGG
    CTCATTGAAC TTATTAGGTC TGTGTTTCAC CTAAAACTGC AGCTATTTTT TCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa57511
    Clone ID Related Accession (Same CDS sequence) XM_017353733.2 , XM_017353733.1
    Accession Version XM_017353733.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3537bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product FYVE and coiled-coil domain-containing protein 1-like isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCACTG GTGCTGCAGT CGGGGACAGT CAGATCCAGA GGATTATCAG AGATCTGCGC 
    GATGCTGTTT CCGAACTGAC AAAGGAGTAT AAAGAGAGCG GTGAGCCCAT TACTGATGAC
    AGCTCCAATC TGCACAAGTT CTCCTACAAG CTGGAGTATC TGCTTCAGTT CGACCAAAAG
    GAGAAAACAA CATTTCTGGG CTCCAGAAAA GACTACTGGG ATTACTTCAG CGACTGCCTG
    GCCAAAATCA AAGGAGCAAA TGATGGAATT CGCTTCGTTA AATCAATTTC AGAGCTGAAG
    ACGTCTCTGG GCAAGGGCAG AGCATTTATC CGTTACTCTC TGGTGCATCA GCGGCTGGCA
    GACACACTCC AGCAGTGCCT CATGAACTCC AGAGTCACCA GTGACTGGTA TAATCCGAGG
    AGTCCGTTTC TCAAACCCCA TCTCAGCGTG GACATCATTA GTTATCTCTA CGAGCTCAAT
    GATGTCCAGT TTGATGTGGC ATCCAGAGGT CATGACCTTG ACGCAGAATG GCCCACTTTT
    GCCAGGAGGA CATTAGGAAT GACTAGCTCT CCCAGTCACT TGTGGAAAGC ACCGAGTCGT
    AGTTCCAGCA TCAACAGTCT GGCCAGCACA TACTCACAGC AGCACCATGA GTTCCCTGGG
    AGTCCAGACT TTGGACCTGG GCTTTTATCA GATATGTCTA TGCAAAACTC CAGCATACTG
    AATGATACCT CAATGAGTGC TATAGACGAA CTCCGTCTGG AGCTTGACCA GTCTGAATTA
    AAGCAAAGAG AGCTTATTGA CCGGATTCAG CAGTTAGGTG ATGAGGGGAG TGAACTGCGT
    GGAGTTGTTG TGGAACTGCA AAGGCAGCTA GATGTTTCTT TAGCGGCCCA AGGTAACCAT
    CAGGAGCTAC AACGTAACCT GGAGGTCTTA ATTGAGAGCG AACATGCACT CTCCCGTGAG
    GTGGAGGTCT TAAGAGATCG AGAAACCAGG AGGGAGGTTT CCCATAAAGA CCTTCAGGAC
    ATGCTGGCAG CAGCGGAGCG TAAAAACGAG GAGCTGATGA CAAGGCTAGA TGGAGTATTG
    GATGAGAAAG GTCAAAGGGC GGCCAGTGAT TTCAACTCAG CCCAGAAGAT TCACGAGCTT
    TTGAATGAAT TAAAAGAGGC TGAGAAGAAG AGGATGGATG CTCTGGCTGA AGGAGAAGAG
    AAGAGGAGGC ATGCTGAACA TCTTGCCGAG GAAGTCAAAG TGAAAGATGA AGCTCTCAAA
    GAGGCTGAGG TGAAAATGGC AGCTTGGATG GAGAAGGGTG AACAACTGCA AACCAGGGCG
    GTGGAGCAGC GTAATTTCAT GGAGAAACTT CAAGGTGCAC TTGCAGTAAG AGAGAAAGAG
    ACCAGCAACC TGCAGAGGCA ACTACGAGAT CTTCAGAACT CTCTGGAGAA CATGGAAAAG
    CAGGCCAATG TGGAGAAGAA GAGGATGCAA GATGATAAAG AAGAGCTTGA GATGAAAATG
    AATGGTCTAG AGGGGCTGTT ACAATCACTT CGGACACAGT TAAAGGTTAA AGAATCTGAT
    CTTCTCTCCA GTACCAAGAG AGTTCATTTC CTGGAGAGGG AATCAGAGAA GCTTAGGAGC
    GAGAATCAGA AGTTGGAGTA TGAGTTGGAG AACAGCACCA AAAAGGAGGC CAAGAAGATT
    GATGAATACA AGGATAGCTG CGCCAAGCTT ATTGAGCAAA ACACTAAGCT TCTGCAGACG
    GTGAATAAGA ATGAAGAGAG CAAGAAAGAG TTGCTTGAAA ACAAATCATC CTTGGAGAGC
    GAGTTGGCGG GATTGAGGGC CTCAGAGAAG CAGTTACGAG CTCAAATCGA TGATGCCAAA
    GTGACTGTGG ATGAACGAGA GCAGCGGCTG AGAGAGGAGA ACAGAAATCT GGATGAGAGT
    CTGCAGAAGG CCAACATGCA GCTGGAGGAA TCCGAGAGCT CAATTCGGCA AAAGGAGCAG
    GAGAACAAGG ACCTCATGGA GGTTCAGGTC ACTCTTAAAT CAGCTCTCGC AGCCATGCAG
    AAAGAGATAC GTGACATAAA CAACCAGATC GGTGAACTTG AGAAGAATCT TGGAGTTGCG
    AGGTGTAACG AAGCAAATCT GAACGCGCAG CTTAAAGATA AGGCCACCCA GCTGGAAGAT
    CGAGAGAAGC TCTGTGAGGA GCTCCAGGGA AGAGTGGAAG AACTGGAAAG TCGGCAGAGG
    GATTTGGAAG TGGAGAAGAC AAAAGCAGAA AGAGCCTTTG TTAAACAAAC AGAGATGATT
    CAGAGTCTTG AAGCACAGAG AAACCTGGCA GAGAAGACAC AACTTGAAAA AAGCACATGT
    CAAGCTAAAG AAACTAAAGA GATGGCCTTA AAATTGACTC TTCTAGAAGA TCAACTGGGA
    TTGAGTGCGA AGGAAGTTTC TAAGCTACAA GAGGAAGTGG TTAATCTCAG GGCTAAACTT
    CACAGTGCTG TGGAGGAGAA GGACAAGACG CAAGCAAAGC TTGAGGTCAC CGAAGCTTCC
    TGTGCTGAAC TGCGGATCTT GACTGAGCAC TTGAAGAAGC AGGCCGAGGA GCAGAATCGG
    CTGCATGTGA GCGAGCTGCT GCAGTCTAGT GAACATGTTG ATAAATTAAC TTCGCAGCTG
    AACCAGGAGA CCTCAGCGCA TGAGAAAACC ACTGCAGCAC TTGCTTCCGC TAAAGAGGAT
    CTAGTGGCTC TCAAGGCCCA GAATGAGCGG ATGGTCCTGG AGAATGCGGA AACCAGAGAG
    AGTCTCCACC GGGTCAACAC TGAAATGGCA GAGTTGGGAA TGACTATTTG CAAGCTCACC
    GCCGAGCGGG AAGAGGCCCG GGAACGTTGG GCCGCTGAAG CTGTACGCAT CCAAGAACTT
    CAGCAGCACG GAGTGAAGGA GACAGAGCGA CTCAATGCCA GCTTGGTGGC TCTGCATCAA
    GAAAACTCCA GTCTGCAGGA AGAGCTCCAG CAAACAGACA AACTGTCAGA AACCATGCTG
    GAGCTAAAGC AGCTGCTGGA CAAAACTGAG GGCGAACGGG ACGCTGCACG GGAAGAGATC
    ACCGCTGTGA AATTCCAGAT GAGCACAGAG AGCATGTCTC TCAAGCACCA AATGAAGAGT
    CTTCAAGAAG AGATTGACGG TTTGAAAGAT CAGCTAGACA CAGAGAGGAA GAAGAAATCA
    GAGCTGGAGG CCAAATTATC CGAACTAGAG GGAGCGAATG TGGAATACTC TCGTCTGATT
    GAGGAGAAGG ACTCGCACAT TACTTACTGT GAGACTTTGC TGCGTGAGAG CGAGTCTGAG
    ACACAGCAAC TGCAGGAAAG AGCATCAAGA TCCAAAGAGG CTCTTAGTGA TGTAGAGAAG
    GAGAGGGAAG AATTGAAGCA GAAATTGGAT CAAGTGTTGA TGGAGACTCA GAACCAGCAT
    CTCCGCATGT CTGCTGAACT GGAGGACCTG GGACAAACTA AAGTCAACCT GGAGGAACGG
    CTCATTGAAC TTATTAGGTC TGTGTTTCAC CTAAAACTGC AGCTATTTTT TCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209222.1
    CDS134..3670
    Translation

    Target ORF information:

    RefSeq Version XM_017353733.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017353733.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCACTG GTGCTGCAGT CGGGGACAGT CAGATCCAGA GGATTATCAG AGATCTGCGC 
    GATGCTGTTT CCGAACTGAC AAAGGAGTAT AAAGAGAGCG GTGAGCCCAT TACTGATGAC
    AGCTCCAATC TGCACAAGTT CTCCTACAAG CTGGAGTATC TGCTTCAGTT CGACCAAAAG
    GAGAAAACAA CATTTCTGGG CTCCAGAAAA GACTACTGGG ATTACTTCAG CGACTGCCTG
    GCCAAAATCA AAGGAGCAAA TGATGGAATT CGCTTCGTTA AATCAATTTC AGAGCTGAAG
    ACGTCTCTGG GCAAGGGCAG AGCATTTATC CGTTACTCTC TGGTGCATCA GCGGCTGGCA
    GACACACTCC AGCAGTGCCT CATGAACTCC AGAGTCACCA GTGACTGGTA TAATCCGAGG
    AGTCCGTTTC TCAAACCCCA TCTCAGCGTG GACATCATTA GTTATCTCTA CGAGCTCAAT
    GATGTCCAGT TTGATGTGGC ATCCAGAGGT CATGACCTTG ACGCAGAATG GCCCACTTTT
    GCCAGGAGGA CATTAGGAAT GACTAGCTCT CCCAGTCACT TGTGGAAAGC ACCGAGTCGT
    AGTTCCAGCA TCAACAGTCT GGCCAGCACA TACTCACAGC AGCACCATGA GTTCCCTGGG
    AGTCCAGACT TTGGACCTGG GCTTTTATCA GATATGTCTA TGCAAAACTC CAGCATACTG
    AATGATACCT CAATGAGTGC TATAGACGAA CTCCGTCTGG AGCTTGACCA GTCTGAATTA
    AAGCAAAGAG AGCTTATTGA CCGGATTCAG CAGTTAGGTG ATGAGGGGAG TGAACTGCGT
    GGAGTTGTTG TGGAACTGCA AAGGCAGCTA GATGTTTCTT TAGCGGCCCA AGGTAACCAT
    CAGGAGCTAC AACGTAACCT GGAGGTCTTA ATTGAGAGCG AACATGCACT CTCCCGTGAG
    GTGGAGGTCT TAAGAGATCG AGAAACCAGG AGGGAGGTTT CCCATAAAGA CCTTCAGGAC
    ATGCTGGCAG CAGCGGAGCG TAAAAACGAG GAGCTGATGA CAAGGCTAGA TGGAGTATTG
    GATGAGAAAG GTCAAAGGGC GGCCAGTGAT TTCAACTCAG CCCAGAAGAT TCACGAGCTT
    TTGAATGAAT TAAAAGAGGC TGAGAAGAAG AGGATGGATG CTCTGGCTGA AGGAGAAGAG
    AAGAGGAGGC ATGCTGAACA TCTTGCCGAG GAAGTCAAAG TGAAAGATGA AGCTCTCAAA
    GAGGCTGAGG TGAAAATGGC AGCTTGGATG GAGAAGGGTG AACAACTGCA AACCAGGGCG
    GTGGAGCAGC GTAATTTCAT GGAGAAACTT CAAGGTGCAC TTGCAGTAAG AGAGAAAGAG
    ACCAGCAACC TGCAGAGGCA ACTACGAGAT CTTCAGAACT CTCTGGAGAA CATGGAAAAG
    CAGGCCAATG TGGAGAAGAA GAGGATGCAA GATGATAAAG AAGAGCTTGA GATGAAAATG
    AATGGTCTAG AGGGGCTGTT ACAATCACTT CGGACACAGT TAAAGGTTAA AGAATCTGAT
    CTTCTCTCCA GTACCAAGAG AGTTCATTTC CTGGAGAGGG AATCAGAGAA GCTTAGGAGC
    GAGAATCAGA AGTTGGAGTA TGAGTTGGAG AACAGCACCA AAAAGGAGGC CAAGAAGATT
    GATGAATACA AGGATAGCTG CGCCAAGCTT ATTGAGCAAA ACACTAAGCT TCTGCAGACG
    GTGAATAAGA ATGAAGAGAG CAAGAAAGAG TTGCTTGAAA ACAAATCATC CTTGGAGAGC
    GAGTTGGCGG GATTGAGGGC CTCAGAGAAG CAGTTACGAG CTCAAATCGA TGATGCCAAA
    GTGACTGTGG ATGAACGAGA GCAGCGGCTG AGAGAGGAGA ACAGAAATCT GGATGAGAGT
    CTGCAGAAGG CCAACATGCA GCTGGAGGAA TCCGAGAGCT CAATTCGGCA AAAGGAGCAG
    GAGAACAAGG ACCTCATGGA GGTTCAGGTC ACTCTTAAAT CAGCTCTCGC AGCCATGCAG
    AAAGAGATAC GTGACATAAA CAACCAGATC GGTGAACTTG AGAAGAATCT TGGAGTTGCG
    AGGTGTAACG AAGCAAATCT GAACGCGCAG CTTAAAGATA AGGCCACCCA GCTGGAAGAT
    CGAGAGAAGC TCTGTGAGGA GCTCCAGGGA AGAGTGGAAG AACTGGAAAG TCGGCAGAGG
    GATTTGGAAG TGGAGAAGAC AAAAGCAGAA AGAGCCTTTG TTAAACAAAC AGAGATGATT
    CAGAGTCTTG AAGCACAGAG AAACCTGGCA GAGAAGACAC AACTTGAAAA AAGCACATGT
    CAAGCTAAAG AAACTAAAGA GATGGCCTTA AAATTGACTC TTCTAGAAGA TCAACTGGGA
    TTGAGTGCGA AGGAAGTTTC TAAGCTACAA GAGGAAGTGG TTAATCTCAG GGCTAAACTT
    CACAGTGCTG TGGAGGAGAA GGACAAGACG CAAGCAAAGC TTGAGGTCAC CGAAGCTTCC
    TGTGCTGAAC TGCGGATCTT GACTGAGCAC TTGAAGAAGC AGGCCGAGGA GCAGAATCGG
    CTGCATGTGA GCGAGCTGCT GCAGTCTAGT GAACATGTTG ATAAATTAAC TTCGCAGCTG
    AACCAGGAGA CCTCAGCGCA TGAGAAAACC ACTGCAGCAC TTGCTTCCGC TAAAGAGGAT
    CTAGTGGCTC TCAAGGCCCA GAATGAGCGG ATGGTCCTGG AGAATGCGGA AACCAGAGAG
    AGTCTCCACC GGGTCAACAC TGAAATGGCA GAGTTGGGAA TGACTATTTG CAAGCTCACC
    GCCGAGCGGG AAGAGGCCCG GGAACGTTGG GCCGCTGAAG CTGTACGCAT CCAAGAACTT
    CAGCAGCACG GAGTGAAGGA GACAGAGCGA CTCAATGCCA GCTTGGTGGC TCTGCATCAA
    GAAAACTCCA GTCTGCAGGA AGAGCTCCAG CAAACAGACA AACTGTCAGA AACCATGCTG
    GAGCTAAAGC AGCTGCTGGA CAAAACTGAG GGCGAACGGG ACGCTGCACG GGAAGAGATC
    ACCGCTGTGA AATTCCAGAT GAGCACAGAG AGCATGTCTC TCAAGCACCA AATGAAGAGT
    CTTCAAGAAG AGATTGACGG TTTGAAAGAT CAGCTAGACA CAGAGAGGAA GAAGAAATCA
    GAGCTGGAGG CCAAATTATC CGAACTAGAG GGAGCGAATG TGGAATACTC TCGTCTGATT
    GAGGAGAAGG ACTCGCACAT TACTTACTGT GAGACTTTGC TGCGTGAGAG CGAGTCTGAG
    ACACAGCAAC TGCAGGAAAG AGCATCAAGA TCCAAAGAGG CTCTTAGTGA TGTAGAGAAG
    GAGAGGGAAG AATTGAAGCA GAAATTGGAT CAAGTGTTGA TGGAGACTCA GAACCAGCAT
    CTCCGCATGT CTGCTGAACT GGAGGACCTG GGACAAACTA AAGTCAACCT GGAGGAACGG
    CTCATTGAAC TTATTAGGTC TGTGTTTCAC CTAAAACTGC AGCTATTTTT TCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44696
    Clone ID Related Accession (Same CDS sequence) XM_009297090.2 , XM_009297091.2 , XM_009297090.3 , XM_009297091.3
    Accession Version XM_009297090.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product FYVE and coiled-coil domain-containing protein 1-like isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297090.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCCACTG GTGCTGCAGT CGGGGACAGT CAGATCCAGA GGATTATCAG AGATCTGCGC 
    GATGCTGTTT CCGAACTGAC AAAGGAGTAT AAAGAGAGCG GTGAGCCCAT TACTGATGAC
    AGCTCCAATC TGCACAAGTT CTCCTACAAG CTGGAGTATC TGCTTCAGTT CGACCAAAAG
    GAGAAAACAA CATTTCTGGG CTCCAGAAAA GACTACTGGG ATTACTTCAG CGACTGCCTG
    GCCAAAATCA AAGGAGCAAA TGATGGAATT CGCTTCGTTA AATCAATTTC AGAGCTGAAG
    ACGTCTCTGG GCAAGGGCAG AGCATTTATC CGTTACTCTC TGGTGCATCA GCGGCTGGCA
    GACACACTCC AGCAGTGCCT CATGAACTCC AGAGTCACCA GTGACTGGTA TAATCCGAGG
    AGTCCGTTTC TCAAACCCCA TCTCAGCGTG GACATCATTA GTTATCTCTA CGAGCTCAAT
    GATGTCCAGT TTGATGTGGC ATCCAGAGGT CATGACCTTG ACGCAGAATG GCCCACTTTT
    GCCAGGAGGA CATTAGGAAT GACTAGCTCT CCCAGTCACT TGTGGAAAGC ACCGAGTCGT
    AGTTCCAGCA TCAACAGTCT GGCCAGCACA TACTCACAGC AGCACCATGA GTTCCCTGGG
    AGTCCAGACT TTGGACCTGG GCTTTTATCA GATATGTCTA TGCAAAACTC CAGCATACTG
    AATGATACCT CAATGAGTGC TATAGACGAA CTCCGTCTGG AGCTTGACCA GTCTGAATTA
    AAGCAAAGAG AGCTTATTGA CCGGATTCAG CAGTTAGGTG ATGAGGGGAG TGAACTGCGT
    GGAGTTGTTG TGGAACTGCA AAGGCAGCTA GATGTTTCTT TAGCGGCCCA AGGTAACCAT
    CAGGAGCTAC AACGTAACCT GGAGGTCTTA ATTGAGAGCG AACATGCACT CTCCCGTGAG
    GTGGAGGTCT TAAGAGATCG AGAAACCAGG AGGGAGGTTT CCCATAAAGA CCTTCAGGAC
    ATGCTGGCAG CAGCGGAGCG TAAAAACGAG GAGCTGATGA CAAGGCTAGA TGGAGTATTG
    GATGAGAAAG GTCAAAGGGC GGCCAGTGAT TTCAACTCAG CCCAGAAGAT TCACGAGCTT
    TTGAATGAAT TAAAAGAGGC TGAGAAGAAG AGGATGGATG CTCTGGCTGA AGGAGAAGAG
    AAGAGGAGGC ATGCTGAACA TCTTGCCGAG GAAGTCAAAG TGAAAGATGA AGCTCTCAAA
    GAGGCTGAGG TGAAAATGGC AGCTTGGATG GAGAAGGGTG AACAACTGCA AACCAGGGCG
    GTGGAGCAGC GTAATTTCAT GGAGAAACTT CAAGGTGCAC TTGCAGTAAG AGAGAAAGAG
    ACCAGCAACC TGCAGAGGCA ACTACGAGAT CTTCAGAACT CTCTGGAGAA CATGGAAAAG
    CAGGCCAATG TGGAGAAGAA GAGGATGCAA GATGATAAAG AAGAGCTTGA GATGAAAATG
    AATGGTCTAG AGGGGCTGTT ACAATCACTT CGGACACAGT TAAAGGTTAA AGAATCTGAT
    CTTCTCTCCA GTACCAAGAG AGTTCATTTC CTGGAGAGGG AATCAGAGAA GCTTAGGAGC
    GAGAATCAGA AGTTGGAGTA TGAGTTGGAG AACAGCACCA AAAAGGAGGC CAAGAAGATT
    GATGAATACA AGGATAGCTG CGCCAAGCTT ATTGAGCAAA ACACTAAGCT TCTGCAGACG
    GTGAATAAGA ATGAAGAGAG CAAGAAAGAG TTGCTTGAAA ACAAATCATC CTTGGAGAGC
    GAGTTGGCGG GATTGAGGGC CTCAGAGAAG CAGTTACGAG CTCAAATCGA TGATGCCAAA
    GTGACTGTGG ATGAACGAGA GCAGCGGCTG AGAGAGGAGA ACAGAAATCT GGATGAGAGT
    CTGCAGAAGG CCAACATGCA GCTGGAGGAA TCCGAGAGCT CAATTCGGCA AAAGGAGCAG
    GAGAACAAGG ACCTCATGGA GGTTCAGGTC ACTCTTAAAT CAGCTCTCGC AGCCATGCAG
    AAAGAGATAC GTGACATAAA CAACCAGATC GGTGAACTTG AGAAGAATCT TGGAGTTGCG
    AGGTGTAACG AAGCAAATCT GAACGCGCAG CTTAAAGATA AGGCCACCCA GCTGGAAGAT
    CGAGAGAAGC TCTGTGAGGA GCTCCAGGGA AGAGTGGAAG AACTGGAAAG TCGGCAGAGG
    GATTTGGAAG TGGAGAAGAC AAAAGCAGAA AGAGCCTTTG TTAAACAAAC AGAGATGATT
    CAGAGTCTTG AAGCACAGAG AAACCTGGCA GAGAAGACAC AACTTGAAAA AAGCACATGT
    CAAGCTAAAG AAACTAAAGA GATGGCCTTA AAATTGACTC TTCTAGAAGA TCAACTGGGA
    TTGAGTGCGA AGGAAGTTTC TAAGCTACAA GAGGAAGTGG TTAATCTCAG GGCTAAACTT
    CACAGTGCTG TGGAGGAGAA GGACAAGACG CAAGCAAAGC TTGAGGTCAC CGAAGCTTCC
    TGTGCTGAAC TGCGGATCTT GACTGAGCAC TTGAAGAAGC AGGCCGAGGA GCAGAATCGG
    CTGCATGTGA GCGAGCTGCT GCAGTCTAGT GAACATGTTG ATAAATTAAC TTCGCAGCTG
    AACCAGGAGA CCTCAGCGCA TGAGAAAACC ACTGCAGCAC TTGCTTCCGC TAAAGAGGAT
    CTAGTGGCTC TCAAGGCCCA GAATGAGCGG ATGGTCCTGG AGAATGCGGA AACCAGAGAG
    AGTCTCCACC GGGTCAACAC TGAAATGGCA GAGTTGGGAA TGACTATTTG CAAGCTCACC
    GCCGAGCGGG AAGAGGCCCG GGAACGTTGG GCCGCTGAAG CTGTACGCAT CCAAGAACTT
    CAGCAGCACG GAGTGAAGGA GACAGAGCGA CTCAATGCCA GCTTGGTGGC TCTGCATCAA
    GAAAACTCCA GTCTGCAGGA AGAGCTCCAG CAAACAGACA AACTGTCAGA AACCATGCTG
    GAGCTAAAGC AGCTGCTGGA CAAAACTGAG GGCGAACGGG ACGCTGCACG GGAAGAGATC
    ACCGCTGTGA AATTCCAGAT GAGCACAGAG AGCATGTCTC TCAAGCACCA AATGAAGAGT
    CTTCAAGAAG AGATTGACGG TTTGAAAGAT CAGCTAGACA CAGAGAGGAA GAAGAAATCA
    GAGCTGGAGG CCAAATTATC CGAACTAGAG GGAGCGAATG TGGAATACTC TCGTCTGATT
    GAGGAGAAGG ACTCGCACAT TACTTACTGT GAGACTTTGC TGCGTGAGAG CGAGTCTGAG
    ACACAGCAAC TGCAGGAAAG AGCATCAAGA TCCAAAGAGG CTCTTAGTGA TGTAGAGAAG
    GAGAGGGAAG AATTGAAGCA GAAATTGGAT CAAGTGTTGA TGGAGACTCA GAACCAGCAT
    CTCCGCATGT CTGCTGAACT GGAGGACCTG GGACAAACTA AAGTCAACCT GGAGGAACGG
    CTCATTGAAC TTATTAGAGA TAAGGACGCT CTGTGGCAGA AGTCTGATGC TCTAGAGTTC
    GAGCAGAAGC TAAGAGCAGA GGAGCAGTGG TGGCTGGTGG ATAAAGAGGC CACACACTGT
    CTGGGCTGCC AGGGTCAGTT CACATGGTGG CTGCGGAGGC ATCACTGCAG GTTGTGTGGC
    CGTATCTTCT GCTACTATTG CAGCAACAAT TACGTGATGA CCAAAAACTC AAAGAAGGAG
    CGCTGCTGTC GAGAGTGTTA CACACAACAC GGCGCTGTGG TGGAGAGATT CACCAAAGCT
    GAACTCAACA GCTCATCAGA CAACCAGCCA TCAAACACTG CCGCTCAGCT GCCACCGCCT
    CCATATATAC CCACACCAAG AGTTACAGTT ACAGATCCTG GTGAGAGGGC TGATGATGGA
    GCGTTTGACA TTATTACAGA CGAGGAAGTG AACCAGATCT ATGACAGCGA CTCGCGCACC
    ACCAATGGAT CACATGAGGA GGAAGGAGGA CAAACCGGAG ATAATAGCGT ATCAAGTGAC
    GTGACGTCTG AAGAACAGGA AGACATGATT TCAGTACAGG ATGCAGAAAT CACCCTCCTC
    AAGTCAGGAG AGCTCAGTGC CTCTGTGCCT CTGGATATAG ATGAGATTCC TCATTTCGGC
    GATGGCTCCC GTGAGCTGTT TGTCAAGTCC AGTTGTTACA GCACCATTCC CATCACAGTG
    GGCGACCGCG GACCCATCAT CAGCTGGGTT TTCTCCTCCG AGCCAAAGAG CATCTCCTTC
    ACTGTGGTGT ACAGAGAGAC ACTTGACACA CCTGTCGAGC AGGCCAAGGT TCTGATTCCC
    CTCACGCGCT GTAACTCACA CAAAGAGACC ATACAGGGAC AACTCAAAGT GCGCAACGCT
    GGAGTCTACA CACTCATCTT CGATAACTCA TTCTCCAGAT TTCTCTCCAA GAAGGTCAAC
    TACCACCTGA CCATTGAGAA GCCCGTCATC TACGATGGAA GTGACTTCCC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295365.1
    CDS134..4627
    Translation

    Target ORF information:

    RefSeq Version XM_009297090.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297090.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCCACTG GTGCTGCAGT CGGGGACAGT CAGATCCAGA GGATTATCAG AGATCTGCGC 
    GATGCTGTTT CCGAACTGAC AAAGGAGTAT AAAGAGAGCG GTGAGCCCAT TACTGATGAC
    AGCTCCAATC TGCACAAGTT CTCCTACAAG CTGGAGTATC TGCTTCAGTT CGACCAAAAG
    GAGAAAACAA CATTTCTGGG CTCCAGAAAA GACTACTGGG ATTACTTCAG CGACTGCCTG
    GCCAAAATCA AAGGAGCAAA TGATGGAATT CGCTTCGTTA AATCAATTTC AGAGCTGAAG
    ACGTCTCTGG GCAAGGGCAG AGCATTTATC CGTTACTCTC TGGTGCATCA GCGGCTGGCA
    GACACACTCC AGCAGTGCCT CATGAACTCC AGAGTCACCA GTGACTGGTA TAATCCGAGG
    AGTCCGTTTC TCAAACCCCA TCTCAGCGTG GACATCATTA GTTATCTCTA CGAGCTCAAT
    GATGTCCAGT TTGATGTGGC ATCCAGAGGT CATGACCTTG ACGCAGAATG GCCCACTTTT
    GCCAGGAGGA CATTAGGAAT GACTAGCTCT CCCAGTCACT TGTGGAAAGC ACCGAGTCGT
    AGTTCCAGCA TCAACAGTCT GGCCAGCACA TACTCACAGC AGCACCATGA GTTCCCTGGG
    AGTCCAGACT TTGGACCTGG GCTTTTATCA GATATGTCTA TGCAAAACTC CAGCATACTG
    AATGATACCT CAATGAGTGC TATAGACGAA CTCCGTCTGG AGCTTGACCA GTCTGAATTA
    AAGCAAAGAG AGCTTATTGA CCGGATTCAG CAGTTAGGTG ATGAGGGGAG TGAACTGCGT
    GGAGTTGTTG TGGAACTGCA AAGGCAGCTA GATGTTTCTT TAGCGGCCCA AGGTAACCAT
    CAGGAGCTAC AACGTAACCT GGAGGTCTTA ATTGAGAGCG AACATGCACT CTCCCGTGAG
    GTGGAGGTCT TAAGAGATCG AGAAACCAGG AGGGAGGTTT CCCATAAAGA CCTTCAGGAC
    ATGCTGGCAG CAGCGGAGCG TAAAAACGAG GAGCTGATGA CAAGGCTAGA TGGAGTATTG
    GATGAGAAAG GTCAAAGGGC GGCCAGTGAT TTCAACTCAG CCCAGAAGAT TCACGAGCTT
    TTGAATGAAT TAAAAGAGGC TGAGAAGAAG AGGATGGATG CTCTGGCTGA AGGAGAAGAG
    AAGAGGAGGC ATGCTGAACA TCTTGCCGAG GAAGTCAAAG TGAAAGATGA AGCTCTCAAA
    GAGGCTGAGG TGAAAATGGC AGCTTGGATG GAGAAGGGTG AACAACTGCA AACCAGGGCG
    GTGGAGCAGC GTAATTTCAT GGAGAAACTT CAAGGTGCAC TTGCAGTAAG AGAGAAAGAG
    ACCAGCAACC TGCAGAGGCA ACTACGAGAT CTTCAGAACT CTCTGGAGAA CATGGAAAAG
    CAGGCCAATG TGGAGAAGAA GAGGATGCAA GATGATAAAG AAGAGCTTGA GATGAAAATG
    AATGGTCTAG AGGGGCTGTT ACAATCACTT CGGACACAGT TAAAGGTTAA AGAATCTGAT
    CTTCTCTCCA GTACCAAGAG AGTTCATTTC CTGGAGAGGG AATCAGAGAA GCTTAGGAGC
    GAGAATCAGA AGTTGGAGTA TGAGTTGGAG AACAGCACCA AAAAGGAGGC CAAGAAGATT
    GATGAATACA AGGATAGCTG CGCCAAGCTT ATTGAGCAAA ACACTAAGCT TCTGCAGACG
    GTGAATAAGA ATGAAGAGAG CAAGAAAGAG TTGCTTGAAA ACAAATCATC CTTGGAGAGC
    GAGTTGGCGG GATTGAGGGC CTCAGAGAAG CAGTTACGAG CTCAAATCGA TGATGCCAAA
    GTGACTGTGG ATGAACGAGA GCAGCGGCTG AGAGAGGAGA ACAGAAATCT GGATGAGAGT
    CTGCAGAAGG CCAACATGCA GCTGGAGGAA TCCGAGAGCT CAATTCGGCA AAAGGAGCAG
    GAGAACAAGG ACCTCATGGA GGTTCAGGTC ACTCTTAAAT CAGCTCTCGC AGCCATGCAG
    AAAGAGATAC GTGACATAAA CAACCAGATC GGTGAACTTG AGAAGAATCT TGGAGTTGCG
    AGGTGTAACG AAGCAAATCT GAACGCGCAG CTTAAAGATA AGGCCACCCA GCTGGAAGAT
    CGAGAGAAGC TCTGTGAGGA GCTCCAGGGA AGAGTGGAAG AACTGGAAAG TCGGCAGAGG
    GATTTGGAAG TGGAGAAGAC AAAAGCAGAA AGAGCCTTTG TTAAACAAAC AGAGATGATT
    CAGAGTCTTG AAGCACAGAG AAACCTGGCA GAGAAGACAC AACTTGAAAA AAGCACATGT
    CAAGCTAAAG AAACTAAAGA GATGGCCTTA AAATTGACTC TTCTAGAAGA TCAACTGGGA
    TTGAGTGCGA AGGAAGTTTC TAAGCTACAA GAGGAAGTGG TTAATCTCAG GGCTAAACTT
    CACAGTGCTG TGGAGGAGAA GGACAAGACG CAAGCAAAGC TTGAGGTCAC CGAAGCTTCC
    TGTGCTGAAC TGCGGATCTT GACTGAGCAC TTGAAGAAGC AGGCCGAGGA GCAGAATCGG
    CTGCATGTGA GCGAGCTGCT GCAGTCTAGT GAACATGTTG ATAAATTAAC TTCGCAGCTG
    AACCAGGAGA CCTCAGCGCA TGAGAAAACC ACTGCAGCAC TTGCTTCCGC TAAAGAGGAT
    CTAGTGGCTC TCAAGGCCCA GAATGAGCGG ATGGTCCTGG AGAATGCGGA AACCAGAGAG
    AGTCTCCACC GGGTCAACAC TGAAATGGCA GAGTTGGGAA TGACTATTTG CAAGCTCACC
    GCCGAGCGGG AAGAGGCCCG GGAACGTTGG GCCGCTGAAG CTGTACGCAT CCAAGAACTT
    CAGCAGCACG GAGTGAAGGA GACAGAGCGA CTCAATGCCA GCTTGGTGGC TCTGCATCAA
    GAAAACTCCA GTCTGCAGGA AGAGCTCCAG CAAACAGACA AACTGTCAGA AACCATGCTG
    GAGCTAAAGC AGCTGCTGGA CAAAACTGAG GGCGAACGGG ACGCTGCACG GGAAGAGATC
    ACCGCTGTGA AATTCCAGAT GAGCACAGAG AGCATGTCTC TCAAGCACCA AATGAAGAGT
    CTTCAAGAAG AGATTGACGG TTTGAAAGAT CAGCTAGACA CAGAGAGGAA GAAGAAATCA
    GAGCTGGAGG CCAAATTATC CGAACTAGAG GGAGCGAATG TGGAATACTC TCGTCTGATT
    GAGGAGAAGG ACTCGCACAT TACTTACTGT GAGACTTTGC TGCGTGAGAG CGAGTCTGAG
    ACACAGCAAC TGCAGGAAAG AGCATCAAGA TCCAAAGAGG CTCTTAGTGA TGTAGAGAAG
    GAGAGGGAAG AATTGAAGCA GAAATTGGAT CAAGTGTTGA TGGAGACTCA GAACCAGCAT
    CTCCGCATGT CTGCTGAACT GGAGGACCTG GGACAAACTA AAGTCAACCT GGAGGAACGG
    CTCATTGAAC TTATTAGAGA TAAGGACGCT CTGTGGCAGA AGTCTGATGC TCTAGAGTTC
    GAGCAGAAGC TAAGAGCAGA GGAGCAGTGG TGGCTGGTGG ATAAAGAGGC CACACACTGT
    CTGGGCTGCC AGGGTCAGTT CACATGGTGG CTGCGGAGGC ATCACTGCAG GTTGTGTGGC
    CGTATCTTCT GCTACTATTG CAGCAACAAT TACGTGATGA CCAAAAACTC AAAGAAGGAG
    CGCTGCTGTC GAGAGTGTTA CACACAACAC GGCGCTGTGG TGGAGAGATT CACCAAAGCT
    GAACTCAACA GCTCATCAGA CAACCAGCCA TCAAACACTG CCGCTCAGCT GCCACCGCCT
    CCATATATAC CCACACCAAG AGTTACAGTT ACAGATCCTG GTGAGAGGGC TGATGATGGA
    GCGTTTGACA TTATTACAGA CGAGGAAGTG AACCAGATCT ATGACAGCGA CTCGCGCACC
    ACCAATGGAT CACATGAGGA GGAAGGAGGA CAAACCGGAG ATAATAGCGT ATCAAGTGAC
    GTGACGTCTG AAGAACAGGA AGACATGATT TCAGTACAGG ATGCAGAAAT CACCCTCCTC
    AAGTCAGGAG AGCTCAGTGC CTCTGTGCCT CTGGATATAG ATGAGATTCC TCATTTCGGC
    GATGGCTCCC GTGAGCTGTT TGTCAAGTCC AGTTGTTACA GCACCATTCC CATCACAGTG
    GGCGACCGCG GACCCATCAT CAGCTGGGTT TTCTCCTCCG AGCCAAAGAG CATCTCCTTC
    ACTGTGGTGT ACAGAGAGAC ACTTGACACA CCTGTCGAGC AGGCCAAGGT TCTGATTCCC
    CTCACGCGCT GTAACTCACA CAAAGAGACC ATACAGGGAC AACTCAAAGT GCGCAACGCT
    GGAGTCTACA CACTCATCTT CGATAACTCA TTCTCCAGAT TTCTCTCCAA GAAGGTCAAC
    TACCACCTGA CCATTGAGAA GCCCGTCATC TACGATGGAA GTGACTTCCC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44696
    Clone ID Related Accession (Same CDS sequence) XM_009297090.2 , XM_009297091.2 , XM_009297090.3 , XM_009297091.3
    Accession Version XM_009297091.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product FYVE and coiled-coil domain-containing protein 1-like isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297091.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCCACTG GTGCTGCAGT CGGGGACAGT CAGATCCAGA GGATTATCAG AGATCTGCGC 
    GATGCTGTTT CCGAACTGAC AAAGGAGTAT AAAGAGAGCG GTGAGCCCAT TACTGATGAC
    AGCTCCAATC TGCACAAGTT CTCCTACAAG CTGGAGTATC TGCTTCAGTT CGACCAAAAG
    GAGAAAACAA CATTTCTGGG CTCCAGAAAA GACTACTGGG ATTACTTCAG CGACTGCCTG
    GCCAAAATCA AAGGAGCAAA TGATGGAATT CGCTTCGTTA AATCAATTTC AGAGCTGAAG
    ACGTCTCTGG GCAAGGGCAG AGCATTTATC CGTTACTCTC TGGTGCATCA GCGGCTGGCA
    GACACACTCC AGCAGTGCCT CATGAACTCC AGAGTCACCA GTGACTGGTA TAATCCGAGG
    AGTCCGTTTC TCAAACCCCA TCTCAGCGTG GACATCATTA GTTATCTCTA CGAGCTCAAT
    GATGTCCAGT TTGATGTGGC ATCCAGAGGT CATGACCTTG ACGCAGAATG GCCCACTTTT
    GCCAGGAGGA CATTAGGAAT GACTAGCTCT CCCAGTCACT TGTGGAAAGC ACCGAGTCGT
    AGTTCCAGCA TCAACAGTCT GGCCAGCACA TACTCACAGC AGCACCATGA GTTCCCTGGG
    AGTCCAGACT TTGGACCTGG GCTTTTATCA GATATGTCTA TGCAAAACTC CAGCATACTG
    AATGATACCT CAATGAGTGC TATAGACGAA CTCCGTCTGG AGCTTGACCA GTCTGAATTA
    AAGCAAAGAG AGCTTATTGA CCGGATTCAG CAGTTAGGTG ATGAGGGGAG TGAACTGCGT
    GGAGTTGTTG TGGAACTGCA AAGGCAGCTA GATGTTTCTT TAGCGGCCCA AGGTAACCAT
    CAGGAGCTAC AACGTAACCT GGAGGTCTTA ATTGAGAGCG AACATGCACT CTCCCGTGAG
    GTGGAGGTCT TAAGAGATCG AGAAACCAGG AGGGAGGTTT CCCATAAAGA CCTTCAGGAC
    ATGCTGGCAG CAGCGGAGCG TAAAAACGAG GAGCTGATGA CAAGGCTAGA TGGAGTATTG
    GATGAGAAAG GTCAAAGGGC GGCCAGTGAT TTCAACTCAG CCCAGAAGAT TCACGAGCTT
    TTGAATGAAT TAAAAGAGGC TGAGAAGAAG AGGATGGATG CTCTGGCTGA AGGAGAAGAG
    AAGAGGAGGC ATGCTGAACA TCTTGCCGAG GAAGTCAAAG TGAAAGATGA AGCTCTCAAA
    GAGGCTGAGG TGAAAATGGC AGCTTGGATG GAGAAGGGTG AACAACTGCA AACCAGGGCG
    GTGGAGCAGC GTAATTTCAT GGAGAAACTT CAAGGTGCAC TTGCAGTAAG AGAGAAAGAG
    ACCAGCAACC TGCAGAGGCA ACTACGAGAT CTTCAGAACT CTCTGGAGAA CATGGAAAAG
    CAGGCCAATG TGGAGAAGAA GAGGATGCAA GATGATAAAG AAGAGCTTGA GATGAAAATG
    AATGGTCTAG AGGGGCTGTT ACAATCACTT CGGACACAGT TAAAGGTTAA AGAATCTGAT
    CTTCTCTCCA GTACCAAGAG AGTTCATTTC CTGGAGAGGG AATCAGAGAA GCTTAGGAGC
    GAGAATCAGA AGTTGGAGTA TGAGTTGGAG AACAGCACCA AAAAGGAGGC CAAGAAGATT
    GATGAATACA AGGATAGCTG CGCCAAGCTT ATTGAGCAAA ACACTAAGCT TCTGCAGACG
    GTGAATAAGA ATGAAGAGAG CAAGAAAGAG TTGCTTGAAA ACAAATCATC CTTGGAGAGC
    GAGTTGGCGG GATTGAGGGC CTCAGAGAAG CAGTTACGAG CTCAAATCGA TGATGCCAAA
    GTGACTGTGG ATGAACGAGA GCAGCGGCTG AGAGAGGAGA ACAGAAATCT GGATGAGAGT
    CTGCAGAAGG CCAACATGCA GCTGGAGGAA TCCGAGAGCT CAATTCGGCA AAAGGAGCAG
    GAGAACAAGG ACCTCATGGA GGTTCAGGTC ACTCTTAAAT CAGCTCTCGC AGCCATGCAG
    AAAGAGATAC GTGACATAAA CAACCAGATC GGTGAACTTG AGAAGAATCT TGGAGTTGCG
    AGGTGTAACG AAGCAAATCT GAACGCGCAG CTTAAAGATA AGGCCACCCA GCTGGAAGAT
    CGAGAGAAGC TCTGTGAGGA GCTCCAGGGA AGAGTGGAAG AACTGGAAAG TCGGCAGAGG
    GATTTGGAAG TGGAGAAGAC AAAAGCAGAA AGAGCCTTTG TTAAACAAAC AGAGATGATT
    CAGAGTCTTG AAGCACAGAG AAACCTGGCA GAGAAGACAC AACTTGAAAA AAGCACATGT
    CAAGCTAAAG AAACTAAAGA GATGGCCTTA AAATTGACTC TTCTAGAAGA TCAACTGGGA
    TTGAGTGCGA AGGAAGTTTC TAAGCTACAA GAGGAAGTGG TTAATCTCAG GGCTAAACTT
    CACAGTGCTG TGGAGGAGAA GGACAAGACG CAAGCAAAGC TTGAGGTCAC CGAAGCTTCC
    TGTGCTGAAC TGCGGATCTT GACTGAGCAC TTGAAGAAGC AGGCCGAGGA GCAGAATCGG
    CTGCATGTGA GCGAGCTGCT GCAGTCTAGT GAACATGTTG ATAAATTAAC TTCGCAGCTG
    AACCAGGAGA CCTCAGCGCA TGAGAAAACC ACTGCAGCAC TTGCTTCCGC TAAAGAGGAT
    CTAGTGGCTC TCAAGGCCCA GAATGAGCGG ATGGTCCTGG AGAATGCGGA AACCAGAGAG
    AGTCTCCACC GGGTCAACAC TGAAATGGCA GAGTTGGGAA TGACTATTTG CAAGCTCACC
    GCCGAGCGGG AAGAGGCCCG GGAACGTTGG GCCGCTGAAG CTGTACGCAT CCAAGAACTT
    CAGCAGCACG GAGTGAAGGA GACAGAGCGA CTCAATGCCA GCTTGGTGGC TCTGCATCAA
    GAAAACTCCA GTCTGCAGGA AGAGCTCCAG CAAACAGACA AACTGTCAGA AACCATGCTG
    GAGCTAAAGC AGCTGCTGGA CAAAACTGAG GGCGAACGGG ACGCTGCACG GGAAGAGATC
    ACCGCTGTGA AATTCCAGAT GAGCACAGAG AGCATGTCTC TCAAGCACCA AATGAAGAGT
    CTTCAAGAAG AGATTGACGG TTTGAAAGAT CAGCTAGACA CAGAGAGGAA GAAGAAATCA
    GAGCTGGAGG CCAAATTATC CGAACTAGAG GGAGCGAATG TGGAATACTC TCGTCTGATT
    GAGGAGAAGG ACTCGCACAT TACTTACTGT GAGACTTTGC TGCGTGAGAG CGAGTCTGAG
    ACACAGCAAC TGCAGGAAAG AGCATCAAGA TCCAAAGAGG CTCTTAGTGA TGTAGAGAAG
    GAGAGGGAAG AATTGAAGCA GAAATTGGAT CAAGTGTTGA TGGAGACTCA GAACCAGCAT
    CTCCGCATGT CTGCTGAACT GGAGGACCTG GGACAAACTA AAGTCAACCT GGAGGAACGG
    CTCATTGAAC TTATTAGAGA TAAGGACGCT CTGTGGCAGA AGTCTGATGC TCTAGAGTTC
    GAGCAGAAGC TAAGAGCAGA GGAGCAGTGG TGGCTGGTGG ATAAAGAGGC CACACACTGT
    CTGGGCTGCC AGGGTCAGTT CACATGGTGG CTGCGGAGGC ATCACTGCAG GTTGTGTGGC
    CGTATCTTCT GCTACTATTG CAGCAACAAT TACGTGATGA CCAAAAACTC AAAGAAGGAG
    CGCTGCTGTC GAGAGTGTTA CACACAACAC GGCGCTGTGG TGGAGAGATT CACCAAAGCT
    GAACTCAACA GCTCATCAGA CAACCAGCCA TCAAACACTG CCGCTCAGCT GCCACCGCCT
    CCATATATAC CCACACCAAG AGTTACAGTT ACAGATCCTG GTGAGAGGGC TGATGATGGA
    GCGTTTGACA TTATTACAGA CGAGGAAGTG AACCAGATCT ATGACAGCGA CTCGCGCACC
    ACCAATGGAT CACATGAGGA GGAAGGAGGA CAAACCGGAG ATAATAGCGT ATCAAGTGAC
    GTGACGTCTG AAGAACAGGA AGACATGATT TCAGTACAGG ATGCAGAAAT CACCCTCCTC
    AAGTCAGGAG AGCTCAGTGC CTCTGTGCCT CTGGATATAG ATGAGATTCC TCATTTCGGC
    GATGGCTCCC GTGAGCTGTT TGTCAAGTCC AGTTGTTACA GCACCATTCC CATCACAGTG
    GGCGACCGCG GACCCATCAT CAGCTGGGTT TTCTCCTCCG AGCCAAAGAG CATCTCCTTC
    ACTGTGGTGT ACAGAGAGAC ACTTGACACA CCTGTCGAGC AGGCCAAGGT TCTGATTCCC
    CTCACGCGCT GTAACTCACA CAAAGAGACC ATACAGGGAC AACTCAAAGT GCGCAACGCT
    GGAGTCTACA CACTCATCTT CGATAACTCA TTCTCCAGAT TTCTCTCCAA GAAGGTCAAC
    TACCACCTGA CCATTGAGAA GCCCGTCATC TACGATGGAA GTGACTTCCC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295366.1
    CDS88..4581
    Translation

    Target ORF information:

    RefSeq Version XM_009297091.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297091.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCCACTG GTGCTGCAGT CGGGGACAGT CAGATCCAGA GGATTATCAG AGATCTGCGC 
    GATGCTGTTT CCGAACTGAC AAAGGAGTAT AAAGAGAGCG GTGAGCCCAT TACTGATGAC
    AGCTCCAATC TGCACAAGTT CTCCTACAAG CTGGAGTATC TGCTTCAGTT CGACCAAAAG
    GAGAAAACAA CATTTCTGGG CTCCAGAAAA GACTACTGGG ATTACTTCAG CGACTGCCTG
    GCCAAAATCA AAGGAGCAAA TGATGGAATT CGCTTCGTTA AATCAATTTC AGAGCTGAAG
    ACGTCTCTGG GCAAGGGCAG AGCATTTATC CGTTACTCTC TGGTGCATCA GCGGCTGGCA
    GACACACTCC AGCAGTGCCT CATGAACTCC AGAGTCACCA GTGACTGGTA TAATCCGAGG
    AGTCCGTTTC TCAAACCCCA TCTCAGCGTG GACATCATTA GTTATCTCTA CGAGCTCAAT
    GATGTCCAGT TTGATGTGGC ATCCAGAGGT CATGACCTTG ACGCAGAATG GCCCACTTTT
    GCCAGGAGGA CATTAGGAAT GACTAGCTCT CCCAGTCACT TGTGGAAAGC ACCGAGTCGT
    AGTTCCAGCA TCAACAGTCT GGCCAGCACA TACTCACAGC AGCACCATGA GTTCCCTGGG
    AGTCCAGACT TTGGACCTGG GCTTTTATCA GATATGTCTA TGCAAAACTC CAGCATACTG
    AATGATACCT CAATGAGTGC TATAGACGAA CTCCGTCTGG AGCTTGACCA GTCTGAATTA
    AAGCAAAGAG AGCTTATTGA CCGGATTCAG CAGTTAGGTG ATGAGGGGAG TGAACTGCGT
    GGAGTTGTTG TGGAACTGCA AAGGCAGCTA GATGTTTCTT TAGCGGCCCA AGGTAACCAT
    CAGGAGCTAC AACGTAACCT GGAGGTCTTA ATTGAGAGCG AACATGCACT CTCCCGTGAG
    GTGGAGGTCT TAAGAGATCG AGAAACCAGG AGGGAGGTTT CCCATAAAGA CCTTCAGGAC
    ATGCTGGCAG CAGCGGAGCG TAAAAACGAG GAGCTGATGA CAAGGCTAGA TGGAGTATTG
    GATGAGAAAG GTCAAAGGGC GGCCAGTGAT TTCAACTCAG CCCAGAAGAT TCACGAGCTT
    TTGAATGAAT TAAAAGAGGC TGAGAAGAAG AGGATGGATG CTCTGGCTGA AGGAGAAGAG
    AAGAGGAGGC ATGCTGAACA TCTTGCCGAG GAAGTCAAAG TGAAAGATGA AGCTCTCAAA
    GAGGCTGAGG TGAAAATGGC AGCTTGGATG GAGAAGGGTG AACAACTGCA AACCAGGGCG
    GTGGAGCAGC GTAATTTCAT GGAGAAACTT CAAGGTGCAC TTGCAGTAAG AGAGAAAGAG
    ACCAGCAACC TGCAGAGGCA ACTACGAGAT CTTCAGAACT CTCTGGAGAA CATGGAAAAG
    CAGGCCAATG TGGAGAAGAA GAGGATGCAA GATGATAAAG AAGAGCTTGA GATGAAAATG
    AATGGTCTAG AGGGGCTGTT ACAATCACTT CGGACACAGT TAAAGGTTAA AGAATCTGAT
    CTTCTCTCCA GTACCAAGAG AGTTCATTTC CTGGAGAGGG AATCAGAGAA GCTTAGGAGC
    GAGAATCAGA AGTTGGAGTA TGAGTTGGAG AACAGCACCA AAAAGGAGGC CAAGAAGATT
    GATGAATACA AGGATAGCTG CGCCAAGCTT ATTGAGCAAA ACACTAAGCT TCTGCAGACG
    GTGAATAAGA ATGAAGAGAG CAAGAAAGAG TTGCTTGAAA ACAAATCATC CTTGGAGAGC
    GAGTTGGCGG GATTGAGGGC CTCAGAGAAG CAGTTACGAG CTCAAATCGA TGATGCCAAA
    GTGACTGTGG ATGAACGAGA GCAGCGGCTG AGAGAGGAGA ACAGAAATCT GGATGAGAGT
    CTGCAGAAGG CCAACATGCA GCTGGAGGAA TCCGAGAGCT CAATTCGGCA AAAGGAGCAG
    GAGAACAAGG ACCTCATGGA GGTTCAGGTC ACTCTTAAAT CAGCTCTCGC AGCCATGCAG
    AAAGAGATAC GTGACATAAA CAACCAGATC GGTGAACTTG AGAAGAATCT TGGAGTTGCG
    AGGTGTAACG AAGCAAATCT GAACGCGCAG CTTAAAGATA AGGCCACCCA GCTGGAAGAT
    CGAGAGAAGC TCTGTGAGGA GCTCCAGGGA AGAGTGGAAG AACTGGAAAG TCGGCAGAGG
    GATTTGGAAG TGGAGAAGAC AAAAGCAGAA AGAGCCTTTG TTAAACAAAC AGAGATGATT
    CAGAGTCTTG AAGCACAGAG AAACCTGGCA GAGAAGACAC AACTTGAAAA AAGCACATGT
    CAAGCTAAAG AAACTAAAGA GATGGCCTTA AAATTGACTC TTCTAGAAGA TCAACTGGGA
    TTGAGTGCGA AGGAAGTTTC TAAGCTACAA GAGGAAGTGG TTAATCTCAG GGCTAAACTT
    CACAGTGCTG TGGAGGAGAA GGACAAGACG CAAGCAAAGC TTGAGGTCAC CGAAGCTTCC
    TGTGCTGAAC TGCGGATCTT GACTGAGCAC TTGAAGAAGC AGGCCGAGGA GCAGAATCGG
    CTGCATGTGA GCGAGCTGCT GCAGTCTAGT GAACATGTTG ATAAATTAAC TTCGCAGCTG
    AACCAGGAGA CCTCAGCGCA TGAGAAAACC ACTGCAGCAC TTGCTTCCGC TAAAGAGGAT
    CTAGTGGCTC TCAAGGCCCA GAATGAGCGG ATGGTCCTGG AGAATGCGGA AACCAGAGAG
    AGTCTCCACC GGGTCAACAC TGAAATGGCA GAGTTGGGAA TGACTATTTG CAAGCTCACC
    GCCGAGCGGG AAGAGGCCCG GGAACGTTGG GCCGCTGAAG CTGTACGCAT CCAAGAACTT
    CAGCAGCACG GAGTGAAGGA GACAGAGCGA CTCAATGCCA GCTTGGTGGC TCTGCATCAA
    GAAAACTCCA GTCTGCAGGA AGAGCTCCAG CAAACAGACA AACTGTCAGA AACCATGCTG
    GAGCTAAAGC AGCTGCTGGA CAAAACTGAG GGCGAACGGG ACGCTGCACG GGAAGAGATC
    ACCGCTGTGA AATTCCAGAT GAGCACAGAG AGCATGTCTC TCAAGCACCA AATGAAGAGT
    CTTCAAGAAG AGATTGACGG TTTGAAAGAT CAGCTAGACA CAGAGAGGAA GAAGAAATCA
    GAGCTGGAGG CCAAATTATC CGAACTAGAG GGAGCGAATG TGGAATACTC TCGTCTGATT
    GAGGAGAAGG ACTCGCACAT TACTTACTGT GAGACTTTGC TGCGTGAGAG CGAGTCTGAG
    ACACAGCAAC TGCAGGAAAG AGCATCAAGA TCCAAAGAGG CTCTTAGTGA TGTAGAGAAG
    GAGAGGGAAG AATTGAAGCA GAAATTGGAT CAAGTGTTGA TGGAGACTCA GAACCAGCAT
    CTCCGCATGT CTGCTGAACT GGAGGACCTG GGACAAACTA AAGTCAACCT GGAGGAACGG
    CTCATTGAAC TTATTAGAGA TAAGGACGCT CTGTGGCAGA AGTCTGATGC TCTAGAGTTC
    GAGCAGAAGC TAAGAGCAGA GGAGCAGTGG TGGCTGGTGG ATAAAGAGGC CACACACTGT
    CTGGGCTGCC AGGGTCAGTT CACATGGTGG CTGCGGAGGC ATCACTGCAG GTTGTGTGGC
    CGTATCTTCT GCTACTATTG CAGCAACAAT TACGTGATGA CCAAAAACTC AAAGAAGGAG
    CGCTGCTGTC GAGAGTGTTA CACACAACAC GGCGCTGTGG TGGAGAGATT CACCAAAGCT
    GAACTCAACA GCTCATCAGA CAACCAGCCA TCAAACACTG CCGCTCAGCT GCCACCGCCT
    CCATATATAC CCACACCAAG AGTTACAGTT ACAGATCCTG GTGAGAGGGC TGATGATGGA
    GCGTTTGACA TTATTACAGA CGAGGAAGTG AACCAGATCT ATGACAGCGA CTCGCGCACC
    ACCAATGGAT CACATGAGGA GGAAGGAGGA CAAACCGGAG ATAATAGCGT ATCAAGTGAC
    GTGACGTCTG AAGAACAGGA AGACATGATT TCAGTACAGG ATGCAGAAAT CACCCTCCTC
    AAGTCAGGAG AGCTCAGTGC CTCTGTGCCT CTGGATATAG ATGAGATTCC TCATTTCGGC
    GATGGCTCCC GTGAGCTGTT TGTCAAGTCC AGTTGTTACA GCACCATTCC CATCACAGTG
    GGCGACCGCG GACCCATCAT CAGCTGGGTT TTCTCCTCCG AGCCAAAGAG CATCTCCTTC
    ACTGTGGTGT ACAGAGAGAC ACTTGACACA CCTGTCGAGC AGGCCAAGGT TCTGATTCCC
    CTCACGCGCT GTAACTCACA CAAAGAGACC ATACAGGGAC AACTCAAAGT GCGCAACGCT
    GGAGTCTACA CACTCATCTT CGATAACTCA TTCTCCAGAT TTCTCTCCAA GAAGGTCAAC
    TACCACCTGA CCATTGAGAA GCCCGTCATC TACGATGGAA GTGACTTCCC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44696
    Clone ID Related Accession (Same CDS sequence) XM_009297090.2 , XM_009297091.2 , XM_009297090.3 , XM_009297091.3
    Accession Version XM_009297090.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product FYVE and coiled-coil domain-containing protein 1-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297090.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCCACTG GTGCTGCAGT CGGGGACAGT CAGATCCAGA GGATTATCAG AGATCTGCGC 
    GATGCTGTTT CCGAACTGAC AAAGGAGTAT AAAGAGAGCG GTGAGCCCAT TACTGATGAC
    AGCTCCAATC TGCACAAGTT CTCCTACAAG CTGGAGTATC TGCTTCAGTT CGACCAAAAG
    GAGAAAACAA CATTTCTGGG CTCCAGAAAA GACTACTGGG ATTACTTCAG CGACTGCCTG
    GCCAAAATCA AAGGAGCAAA TGATGGAATT CGCTTCGTTA AATCAATTTC AGAGCTGAAG
    ACGTCTCTGG GCAAGGGCAG AGCATTTATC CGTTACTCTC TGGTGCATCA GCGGCTGGCA
    GACACACTCC AGCAGTGCCT CATGAACTCC AGAGTCACCA GTGACTGGTA TAATCCGAGG
    AGTCCGTTTC TCAAACCCCA TCTCAGCGTG GACATCATTA GTTATCTCTA CGAGCTCAAT
    GATGTCCAGT TTGATGTGGC ATCCAGAGGT CATGACCTTG ACGCAGAATG GCCCACTTTT
    GCCAGGAGGA CATTAGGAAT GACTAGCTCT CCCAGTCACT TGTGGAAAGC ACCGAGTCGT
    AGTTCCAGCA TCAACAGTCT GGCCAGCACA TACTCACAGC AGCACCATGA GTTCCCTGGG
    AGTCCAGACT TTGGACCTGG GCTTTTATCA GATATGTCTA TGCAAAACTC CAGCATACTG
    AATGATACCT CAATGAGTGC TATAGACGAA CTCCGTCTGG AGCTTGACCA GTCTGAATTA
    AAGCAAAGAG AGCTTATTGA CCGGATTCAG CAGTTAGGTG ATGAGGGGAG TGAACTGCGT
    GGAGTTGTTG TGGAACTGCA AAGGCAGCTA GATGTTTCTT TAGCGGCCCA AGGTAACCAT
    CAGGAGCTAC AACGTAACCT GGAGGTCTTA ATTGAGAGCG AACATGCACT CTCCCGTGAG
    GTGGAGGTCT TAAGAGATCG AGAAACCAGG AGGGAGGTTT CCCATAAAGA CCTTCAGGAC
    ATGCTGGCAG CAGCGGAGCG TAAAAACGAG GAGCTGATGA CAAGGCTAGA TGGAGTATTG
    GATGAGAAAG GTCAAAGGGC GGCCAGTGAT TTCAACTCAG CCCAGAAGAT TCACGAGCTT
    TTGAATGAAT TAAAAGAGGC TGAGAAGAAG AGGATGGATG CTCTGGCTGA AGGAGAAGAG
    AAGAGGAGGC ATGCTGAACA TCTTGCCGAG GAAGTCAAAG TGAAAGATGA AGCTCTCAAA
    GAGGCTGAGG TGAAAATGGC AGCTTGGATG GAGAAGGGTG AACAACTGCA AACCAGGGCG
    GTGGAGCAGC GTAATTTCAT GGAGAAACTT CAAGGTGCAC TTGCAGTAAG AGAGAAAGAG
    ACCAGCAACC TGCAGAGGCA ACTACGAGAT CTTCAGAACT CTCTGGAGAA CATGGAAAAG
    CAGGCCAATG TGGAGAAGAA GAGGATGCAA GATGATAAAG AAGAGCTTGA GATGAAAATG
    AATGGTCTAG AGGGGCTGTT ACAATCACTT CGGACACAGT TAAAGGTTAA AGAATCTGAT
    CTTCTCTCCA GTACCAAGAG AGTTCATTTC CTGGAGAGGG AATCAGAGAA GCTTAGGAGC
    GAGAATCAGA AGTTGGAGTA TGAGTTGGAG AACAGCACCA AAAAGGAGGC CAAGAAGATT
    GATGAATACA AGGATAGCTG CGCCAAGCTT ATTGAGCAAA ACACTAAGCT TCTGCAGACG
    GTGAATAAGA ATGAAGAGAG CAAGAAAGAG TTGCTTGAAA ACAAATCATC CTTGGAGAGC
    GAGTTGGCGG GATTGAGGGC CTCAGAGAAG CAGTTACGAG CTCAAATCGA TGATGCCAAA
    GTGACTGTGG ATGAACGAGA GCAGCGGCTG AGAGAGGAGA ACAGAAATCT GGATGAGAGT
    CTGCAGAAGG CCAACATGCA GCTGGAGGAA TCCGAGAGCT CAATTCGGCA AAAGGAGCAG
    GAGAACAAGG ACCTCATGGA GGTTCAGGTC ACTCTTAAAT CAGCTCTCGC AGCCATGCAG
    AAAGAGATAC GTGACATAAA CAACCAGATC GGTGAACTTG AGAAGAATCT TGGAGTTGCG
    AGGTGTAACG AAGCAAATCT GAACGCGCAG CTTAAAGATA AGGCCACCCA GCTGGAAGAT
    CGAGAGAAGC TCTGTGAGGA GCTCCAGGGA AGAGTGGAAG AACTGGAAAG TCGGCAGAGG
    GATTTGGAAG TGGAGAAGAC AAAAGCAGAA AGAGCCTTTG TTAAACAAAC AGAGATGATT
    CAGAGTCTTG AAGCACAGAG AAACCTGGCA GAGAAGACAC AACTTGAAAA AAGCACATGT
    CAAGCTAAAG AAACTAAAGA GATGGCCTTA AAATTGACTC TTCTAGAAGA TCAACTGGGA
    TTGAGTGCGA AGGAAGTTTC TAAGCTACAA GAGGAAGTGG TTAATCTCAG GGCTAAACTT
    CACAGTGCTG TGGAGGAGAA GGACAAGACG CAAGCAAAGC TTGAGGTCAC CGAAGCTTCC
    TGTGCTGAAC TGCGGATCTT GACTGAGCAC TTGAAGAAGC AGGCCGAGGA GCAGAATCGG
    CTGCATGTGA GCGAGCTGCT GCAGTCTAGT GAACATGTTG ATAAATTAAC TTCGCAGCTG
    AACCAGGAGA CCTCAGCGCA TGAGAAAACC ACTGCAGCAC TTGCTTCCGC TAAAGAGGAT
    CTAGTGGCTC TCAAGGCCCA GAATGAGCGG ATGGTCCTGG AGAATGCGGA AACCAGAGAG
    AGTCTCCACC GGGTCAACAC TGAAATGGCA GAGTTGGGAA TGACTATTTG CAAGCTCACC
    GCCGAGCGGG AAGAGGCCCG GGAACGTTGG GCCGCTGAAG CTGTACGCAT CCAAGAACTT
    CAGCAGCACG GAGTGAAGGA GACAGAGCGA CTCAATGCCA GCTTGGTGGC TCTGCATCAA
    GAAAACTCCA GTCTGCAGGA AGAGCTCCAG CAAACAGACA AACTGTCAGA AACCATGCTG
    GAGCTAAAGC AGCTGCTGGA CAAAACTGAG GGCGAACGGG ACGCTGCACG GGAAGAGATC
    ACCGCTGTGA AATTCCAGAT GAGCACAGAG AGCATGTCTC TCAAGCACCA AATGAAGAGT
    CTTCAAGAAG AGATTGACGG TTTGAAAGAT CAGCTAGACA CAGAGAGGAA GAAGAAATCA
    GAGCTGGAGG CCAAATTATC CGAACTAGAG GGAGCGAATG TGGAATACTC TCGTCTGATT
    GAGGAGAAGG ACTCGCACAT TACTTACTGT GAGACTTTGC TGCGTGAGAG CGAGTCTGAG
    ACACAGCAAC TGCAGGAAAG AGCATCAAGA TCCAAAGAGG CTCTTAGTGA TGTAGAGAAG
    GAGAGGGAAG AATTGAAGCA GAAATTGGAT CAAGTGTTGA TGGAGACTCA GAACCAGCAT
    CTCCGCATGT CTGCTGAACT GGAGGACCTG GGACAAACTA AAGTCAACCT GGAGGAACGG
    CTCATTGAAC TTATTAGAGA TAAGGACGCT CTGTGGCAGA AGTCTGATGC TCTAGAGTTC
    GAGCAGAAGC TAAGAGCAGA GGAGCAGTGG TGGCTGGTGG ATAAAGAGGC CACACACTGT
    CTGGGCTGCC AGGGTCAGTT CACATGGTGG CTGCGGAGGC ATCACTGCAG GTTGTGTGGC
    CGTATCTTCT GCTACTATTG CAGCAACAAT TACGTGATGA CCAAAAACTC AAAGAAGGAG
    CGCTGCTGTC GAGAGTGTTA CACACAACAC GGCGCTGTGG TGGAGAGATT CACCAAAGCT
    GAACTCAACA GCTCATCAGA CAACCAGCCA TCAAACACTG CCGCTCAGCT GCCACCGCCT
    CCATATATAC CCACACCAAG AGTTACAGTT ACAGATCCTG GTGAGAGGGC TGATGATGGA
    GCGTTTGACA TTATTACAGA CGAGGAAGTG AACCAGATCT ATGACAGCGA CTCGCGCACC
    ACCAATGGAT CACATGAGGA GGAAGGAGGA CAAACCGGAG ATAATAGCGT ATCAAGTGAC
    GTGACGTCTG AAGAACAGGA AGACATGATT TCAGTACAGG ATGCAGAAAT CACCCTCCTC
    AAGTCAGGAG AGCTCAGTGC CTCTGTGCCT CTGGATATAG ATGAGATTCC TCATTTCGGC
    GATGGCTCCC GTGAGCTGTT TGTCAAGTCC AGTTGTTACA GCACCATTCC CATCACAGTG
    GGCGACCGCG GACCCATCAT CAGCTGGGTT TTCTCCTCCG AGCCAAAGAG CATCTCCTTC
    ACTGTGGTGT ACAGAGAGAC ACTTGACACA CCTGTCGAGC AGGCCAAGGT TCTGATTCCC
    CTCACGCGCT GTAACTCACA CAAAGAGACC ATACAGGGAC AACTCAAAGT GCGCAACGCT
    GGAGTCTACA CACTCATCTT CGATAACTCA TTCTCCAGAT TTCTCTCCAA GAAGGTCAAC
    TACCACCTGA CCATTGAGAA GCCCGTCATC TACGATGGAA GTGACTTCCC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295365.1
    CDS156..4649
    Translation

    Target ORF information:

    RefSeq Version XM_009297090.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297090.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCCACTG GTGCTGCAGT CGGGGACAGT CAGATCCAGA GGATTATCAG AGATCTGCGC 
    GATGCTGTTT CCGAACTGAC AAAGGAGTAT AAAGAGAGCG GTGAGCCCAT TACTGATGAC
    AGCTCCAATC TGCACAAGTT CTCCTACAAG CTGGAGTATC TGCTTCAGTT CGACCAAAAG
    GAGAAAACAA CATTTCTGGG CTCCAGAAAA GACTACTGGG ATTACTTCAG CGACTGCCTG
    GCCAAAATCA AAGGAGCAAA TGATGGAATT CGCTTCGTTA AATCAATTTC AGAGCTGAAG
    ACGTCTCTGG GCAAGGGCAG AGCATTTATC CGTTACTCTC TGGTGCATCA GCGGCTGGCA
    GACACACTCC AGCAGTGCCT CATGAACTCC AGAGTCACCA GTGACTGGTA TAATCCGAGG
    AGTCCGTTTC TCAAACCCCA TCTCAGCGTG GACATCATTA GTTATCTCTA CGAGCTCAAT
    GATGTCCAGT TTGATGTGGC ATCCAGAGGT CATGACCTTG ACGCAGAATG GCCCACTTTT
    GCCAGGAGGA CATTAGGAAT GACTAGCTCT CCCAGTCACT TGTGGAAAGC ACCGAGTCGT
    AGTTCCAGCA TCAACAGTCT GGCCAGCACA TACTCACAGC AGCACCATGA GTTCCCTGGG
    AGTCCAGACT TTGGACCTGG GCTTTTATCA GATATGTCTA TGCAAAACTC CAGCATACTG
    AATGATACCT CAATGAGTGC TATAGACGAA CTCCGTCTGG AGCTTGACCA GTCTGAATTA
    AAGCAAAGAG AGCTTATTGA CCGGATTCAG CAGTTAGGTG ATGAGGGGAG TGAACTGCGT
    GGAGTTGTTG TGGAACTGCA AAGGCAGCTA GATGTTTCTT TAGCGGCCCA AGGTAACCAT
    CAGGAGCTAC AACGTAACCT GGAGGTCTTA ATTGAGAGCG AACATGCACT CTCCCGTGAG
    GTGGAGGTCT TAAGAGATCG AGAAACCAGG AGGGAGGTTT CCCATAAAGA CCTTCAGGAC
    ATGCTGGCAG CAGCGGAGCG TAAAAACGAG GAGCTGATGA CAAGGCTAGA TGGAGTATTG
    GATGAGAAAG GTCAAAGGGC GGCCAGTGAT TTCAACTCAG CCCAGAAGAT TCACGAGCTT
    TTGAATGAAT TAAAAGAGGC TGAGAAGAAG AGGATGGATG CTCTGGCTGA AGGAGAAGAG
    AAGAGGAGGC ATGCTGAACA TCTTGCCGAG GAAGTCAAAG TGAAAGATGA AGCTCTCAAA
    GAGGCTGAGG TGAAAATGGC AGCTTGGATG GAGAAGGGTG AACAACTGCA AACCAGGGCG
    GTGGAGCAGC GTAATTTCAT GGAGAAACTT CAAGGTGCAC TTGCAGTAAG AGAGAAAGAG
    ACCAGCAACC TGCAGAGGCA ACTACGAGAT CTTCAGAACT CTCTGGAGAA CATGGAAAAG
    CAGGCCAATG TGGAGAAGAA GAGGATGCAA GATGATAAAG AAGAGCTTGA GATGAAAATG
    AATGGTCTAG AGGGGCTGTT ACAATCACTT CGGACACAGT TAAAGGTTAA AGAATCTGAT
    CTTCTCTCCA GTACCAAGAG AGTTCATTTC CTGGAGAGGG AATCAGAGAA GCTTAGGAGC
    GAGAATCAGA AGTTGGAGTA TGAGTTGGAG AACAGCACCA AAAAGGAGGC CAAGAAGATT
    GATGAATACA AGGATAGCTG CGCCAAGCTT ATTGAGCAAA ACACTAAGCT TCTGCAGACG
    GTGAATAAGA ATGAAGAGAG CAAGAAAGAG TTGCTTGAAA ACAAATCATC CTTGGAGAGC
    GAGTTGGCGG GATTGAGGGC CTCAGAGAAG CAGTTACGAG CTCAAATCGA TGATGCCAAA
    GTGACTGTGG ATGAACGAGA GCAGCGGCTG AGAGAGGAGA ACAGAAATCT GGATGAGAGT
    CTGCAGAAGG CCAACATGCA GCTGGAGGAA TCCGAGAGCT CAATTCGGCA AAAGGAGCAG
    GAGAACAAGG ACCTCATGGA GGTTCAGGTC ACTCTTAAAT CAGCTCTCGC AGCCATGCAG
    AAAGAGATAC GTGACATAAA CAACCAGATC GGTGAACTTG AGAAGAATCT TGGAGTTGCG
    AGGTGTAACG AAGCAAATCT GAACGCGCAG CTTAAAGATA AGGCCACCCA GCTGGAAGAT
    CGAGAGAAGC TCTGTGAGGA GCTCCAGGGA AGAGTGGAAG AACTGGAAAG TCGGCAGAGG
    GATTTGGAAG TGGAGAAGAC AAAAGCAGAA AGAGCCTTTG TTAAACAAAC AGAGATGATT
    CAGAGTCTTG AAGCACAGAG AAACCTGGCA GAGAAGACAC AACTTGAAAA AAGCACATGT
    CAAGCTAAAG AAACTAAAGA GATGGCCTTA AAATTGACTC TTCTAGAAGA TCAACTGGGA
    TTGAGTGCGA AGGAAGTTTC TAAGCTACAA GAGGAAGTGG TTAATCTCAG GGCTAAACTT
    CACAGTGCTG TGGAGGAGAA GGACAAGACG CAAGCAAAGC TTGAGGTCAC CGAAGCTTCC
    TGTGCTGAAC TGCGGATCTT GACTGAGCAC TTGAAGAAGC AGGCCGAGGA GCAGAATCGG
    CTGCATGTGA GCGAGCTGCT GCAGTCTAGT GAACATGTTG ATAAATTAAC TTCGCAGCTG
    AACCAGGAGA CCTCAGCGCA TGAGAAAACC ACTGCAGCAC TTGCTTCCGC TAAAGAGGAT
    CTAGTGGCTC TCAAGGCCCA GAATGAGCGG ATGGTCCTGG AGAATGCGGA AACCAGAGAG
    AGTCTCCACC GGGTCAACAC TGAAATGGCA GAGTTGGGAA TGACTATTTG CAAGCTCACC
    GCCGAGCGGG AAGAGGCCCG GGAACGTTGG GCCGCTGAAG CTGTACGCAT CCAAGAACTT
    CAGCAGCACG GAGTGAAGGA GACAGAGCGA CTCAATGCCA GCTTGGTGGC TCTGCATCAA
    GAAAACTCCA GTCTGCAGGA AGAGCTCCAG CAAACAGACA AACTGTCAGA AACCATGCTG
    GAGCTAAAGC AGCTGCTGGA CAAAACTGAG GGCGAACGGG ACGCTGCACG GGAAGAGATC
    ACCGCTGTGA AATTCCAGAT GAGCACAGAG AGCATGTCTC TCAAGCACCA AATGAAGAGT
    CTTCAAGAAG AGATTGACGG TTTGAAAGAT CAGCTAGACA CAGAGAGGAA GAAGAAATCA
    GAGCTGGAGG CCAAATTATC CGAACTAGAG GGAGCGAATG TGGAATACTC TCGTCTGATT
    GAGGAGAAGG ACTCGCACAT TACTTACTGT GAGACTTTGC TGCGTGAGAG CGAGTCTGAG
    ACACAGCAAC TGCAGGAAAG AGCATCAAGA TCCAAAGAGG CTCTTAGTGA TGTAGAGAAG
    GAGAGGGAAG AATTGAAGCA GAAATTGGAT CAAGTGTTGA TGGAGACTCA GAACCAGCAT
    CTCCGCATGT CTGCTGAACT GGAGGACCTG GGACAAACTA AAGTCAACCT GGAGGAACGG
    CTCATTGAAC TTATTAGAGA TAAGGACGCT CTGTGGCAGA AGTCTGATGC TCTAGAGTTC
    GAGCAGAAGC TAAGAGCAGA GGAGCAGTGG TGGCTGGTGG ATAAAGAGGC CACACACTGT
    CTGGGCTGCC AGGGTCAGTT CACATGGTGG CTGCGGAGGC ATCACTGCAG GTTGTGTGGC
    CGTATCTTCT GCTACTATTG CAGCAACAAT TACGTGATGA CCAAAAACTC AAAGAAGGAG
    CGCTGCTGTC GAGAGTGTTA CACACAACAC GGCGCTGTGG TGGAGAGATT CACCAAAGCT
    GAACTCAACA GCTCATCAGA CAACCAGCCA TCAAACACTG CCGCTCAGCT GCCACCGCCT
    CCATATATAC CCACACCAAG AGTTACAGTT ACAGATCCTG GTGAGAGGGC TGATGATGGA
    GCGTTTGACA TTATTACAGA CGAGGAAGTG AACCAGATCT ATGACAGCGA CTCGCGCACC
    ACCAATGGAT CACATGAGGA GGAAGGAGGA CAAACCGGAG ATAATAGCGT ATCAAGTGAC
    GTGACGTCTG AAGAACAGGA AGACATGATT TCAGTACAGG ATGCAGAAAT CACCCTCCTC
    AAGTCAGGAG AGCTCAGTGC CTCTGTGCCT CTGGATATAG ATGAGATTCC TCATTTCGGC
    GATGGCTCCC GTGAGCTGTT TGTCAAGTCC AGTTGTTACA GCACCATTCC CATCACAGTG
    GGCGACCGCG GACCCATCAT CAGCTGGGTT TTCTCCTCCG AGCCAAAGAG CATCTCCTTC
    ACTGTGGTGT ACAGAGAGAC ACTTGACACA CCTGTCGAGC AGGCCAAGGT TCTGATTCCC
    CTCACGCGCT GTAACTCACA CAAAGAGACC ATACAGGGAC AACTCAAAGT GCGCAACGCT
    GGAGTCTACA CACTCATCTT CGATAACTCA TTCTCCAGAT TTCTCTCCAA GAAGGTCAAC
    TACCACCTGA CCATTGAGAA GCCCGTCATC TACGATGGAA GTGACTTCCC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44696
    Clone ID Related Accession (Same CDS sequence) XM_009297090.2 , XM_009297091.2 , XM_009297090.3 , XM_009297091.3
    Accession Version XM_009297091.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product FYVE and coiled-coil domain-containing protein 1-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297091.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCCACTG GTGCTGCAGT CGGGGACAGT CAGATCCAGA GGATTATCAG AGATCTGCGC 
    GATGCTGTTT CCGAACTGAC AAAGGAGTAT AAAGAGAGCG GTGAGCCCAT TACTGATGAC
    AGCTCCAATC TGCACAAGTT CTCCTACAAG CTGGAGTATC TGCTTCAGTT CGACCAAAAG
    GAGAAAACAA CATTTCTGGG CTCCAGAAAA GACTACTGGG ATTACTTCAG CGACTGCCTG
    GCCAAAATCA AAGGAGCAAA TGATGGAATT CGCTTCGTTA AATCAATTTC AGAGCTGAAG
    ACGTCTCTGG GCAAGGGCAG AGCATTTATC CGTTACTCTC TGGTGCATCA GCGGCTGGCA
    GACACACTCC AGCAGTGCCT CATGAACTCC AGAGTCACCA GTGACTGGTA TAATCCGAGG
    AGTCCGTTTC TCAAACCCCA TCTCAGCGTG GACATCATTA GTTATCTCTA CGAGCTCAAT
    GATGTCCAGT TTGATGTGGC ATCCAGAGGT CATGACCTTG ACGCAGAATG GCCCACTTTT
    GCCAGGAGGA CATTAGGAAT GACTAGCTCT CCCAGTCACT TGTGGAAAGC ACCGAGTCGT
    AGTTCCAGCA TCAACAGTCT GGCCAGCACA TACTCACAGC AGCACCATGA GTTCCCTGGG
    AGTCCAGACT TTGGACCTGG GCTTTTATCA GATATGTCTA TGCAAAACTC CAGCATACTG
    AATGATACCT CAATGAGTGC TATAGACGAA CTCCGTCTGG AGCTTGACCA GTCTGAATTA
    AAGCAAAGAG AGCTTATTGA CCGGATTCAG CAGTTAGGTG ATGAGGGGAG TGAACTGCGT
    GGAGTTGTTG TGGAACTGCA AAGGCAGCTA GATGTTTCTT TAGCGGCCCA AGGTAACCAT
    CAGGAGCTAC AACGTAACCT GGAGGTCTTA ATTGAGAGCG AACATGCACT CTCCCGTGAG
    GTGGAGGTCT TAAGAGATCG AGAAACCAGG AGGGAGGTTT CCCATAAAGA CCTTCAGGAC
    ATGCTGGCAG CAGCGGAGCG TAAAAACGAG GAGCTGATGA CAAGGCTAGA TGGAGTATTG
    GATGAGAAAG GTCAAAGGGC GGCCAGTGAT TTCAACTCAG CCCAGAAGAT TCACGAGCTT
    TTGAATGAAT TAAAAGAGGC TGAGAAGAAG AGGATGGATG CTCTGGCTGA AGGAGAAGAG
    AAGAGGAGGC ATGCTGAACA TCTTGCCGAG GAAGTCAAAG TGAAAGATGA AGCTCTCAAA
    GAGGCTGAGG TGAAAATGGC AGCTTGGATG GAGAAGGGTG AACAACTGCA AACCAGGGCG
    GTGGAGCAGC GTAATTTCAT GGAGAAACTT CAAGGTGCAC TTGCAGTAAG AGAGAAAGAG
    ACCAGCAACC TGCAGAGGCA ACTACGAGAT CTTCAGAACT CTCTGGAGAA CATGGAAAAG
    CAGGCCAATG TGGAGAAGAA GAGGATGCAA GATGATAAAG AAGAGCTTGA GATGAAAATG
    AATGGTCTAG AGGGGCTGTT ACAATCACTT CGGACACAGT TAAAGGTTAA AGAATCTGAT
    CTTCTCTCCA GTACCAAGAG AGTTCATTTC CTGGAGAGGG AATCAGAGAA GCTTAGGAGC
    GAGAATCAGA AGTTGGAGTA TGAGTTGGAG AACAGCACCA AAAAGGAGGC CAAGAAGATT
    GATGAATACA AGGATAGCTG CGCCAAGCTT ATTGAGCAAA ACACTAAGCT TCTGCAGACG
    GTGAATAAGA ATGAAGAGAG CAAGAAAGAG TTGCTTGAAA ACAAATCATC CTTGGAGAGC
    GAGTTGGCGG GATTGAGGGC CTCAGAGAAG CAGTTACGAG CTCAAATCGA TGATGCCAAA
    GTGACTGTGG ATGAACGAGA GCAGCGGCTG AGAGAGGAGA ACAGAAATCT GGATGAGAGT
    CTGCAGAAGG CCAACATGCA GCTGGAGGAA TCCGAGAGCT CAATTCGGCA AAAGGAGCAG
    GAGAACAAGG ACCTCATGGA GGTTCAGGTC ACTCTTAAAT CAGCTCTCGC AGCCATGCAG
    AAAGAGATAC GTGACATAAA CAACCAGATC GGTGAACTTG AGAAGAATCT TGGAGTTGCG
    AGGTGTAACG AAGCAAATCT GAACGCGCAG CTTAAAGATA AGGCCACCCA GCTGGAAGAT
    CGAGAGAAGC TCTGTGAGGA GCTCCAGGGA AGAGTGGAAG AACTGGAAAG TCGGCAGAGG
    GATTTGGAAG TGGAGAAGAC AAAAGCAGAA AGAGCCTTTG TTAAACAAAC AGAGATGATT
    CAGAGTCTTG AAGCACAGAG AAACCTGGCA GAGAAGACAC AACTTGAAAA AAGCACATGT
    CAAGCTAAAG AAACTAAAGA GATGGCCTTA AAATTGACTC TTCTAGAAGA TCAACTGGGA
    TTGAGTGCGA AGGAAGTTTC TAAGCTACAA GAGGAAGTGG TTAATCTCAG GGCTAAACTT
    CACAGTGCTG TGGAGGAGAA GGACAAGACG CAAGCAAAGC TTGAGGTCAC CGAAGCTTCC
    TGTGCTGAAC TGCGGATCTT GACTGAGCAC TTGAAGAAGC AGGCCGAGGA GCAGAATCGG
    CTGCATGTGA GCGAGCTGCT GCAGTCTAGT GAACATGTTG ATAAATTAAC TTCGCAGCTG
    AACCAGGAGA CCTCAGCGCA TGAGAAAACC ACTGCAGCAC TTGCTTCCGC TAAAGAGGAT
    CTAGTGGCTC TCAAGGCCCA GAATGAGCGG ATGGTCCTGG AGAATGCGGA AACCAGAGAG
    AGTCTCCACC GGGTCAACAC TGAAATGGCA GAGTTGGGAA TGACTATTTG CAAGCTCACC
    GCCGAGCGGG AAGAGGCCCG GGAACGTTGG GCCGCTGAAG CTGTACGCAT CCAAGAACTT
    CAGCAGCACG GAGTGAAGGA GACAGAGCGA CTCAATGCCA GCTTGGTGGC TCTGCATCAA
    GAAAACTCCA GTCTGCAGGA AGAGCTCCAG CAAACAGACA AACTGTCAGA AACCATGCTG
    GAGCTAAAGC AGCTGCTGGA CAAAACTGAG GGCGAACGGG ACGCTGCACG GGAAGAGATC
    ACCGCTGTGA AATTCCAGAT GAGCACAGAG AGCATGTCTC TCAAGCACCA AATGAAGAGT
    CTTCAAGAAG AGATTGACGG TTTGAAAGAT CAGCTAGACA CAGAGAGGAA GAAGAAATCA
    GAGCTGGAGG CCAAATTATC CGAACTAGAG GGAGCGAATG TGGAATACTC TCGTCTGATT
    GAGGAGAAGG ACTCGCACAT TACTTACTGT GAGACTTTGC TGCGTGAGAG CGAGTCTGAG
    ACACAGCAAC TGCAGGAAAG AGCATCAAGA TCCAAAGAGG CTCTTAGTGA TGTAGAGAAG
    GAGAGGGAAG AATTGAAGCA GAAATTGGAT CAAGTGTTGA TGGAGACTCA GAACCAGCAT
    CTCCGCATGT CTGCTGAACT GGAGGACCTG GGACAAACTA AAGTCAACCT GGAGGAACGG
    CTCATTGAAC TTATTAGAGA TAAGGACGCT CTGTGGCAGA AGTCTGATGC TCTAGAGTTC
    GAGCAGAAGC TAAGAGCAGA GGAGCAGTGG TGGCTGGTGG ATAAAGAGGC CACACACTGT
    CTGGGCTGCC AGGGTCAGTT CACATGGTGG CTGCGGAGGC ATCACTGCAG GTTGTGTGGC
    CGTATCTTCT GCTACTATTG CAGCAACAAT TACGTGATGA CCAAAAACTC AAAGAAGGAG
    CGCTGCTGTC GAGAGTGTTA CACACAACAC GGCGCTGTGG TGGAGAGATT CACCAAAGCT
    GAACTCAACA GCTCATCAGA CAACCAGCCA TCAAACACTG CCGCTCAGCT GCCACCGCCT
    CCATATATAC CCACACCAAG AGTTACAGTT ACAGATCCTG GTGAGAGGGC TGATGATGGA
    GCGTTTGACA TTATTACAGA CGAGGAAGTG AACCAGATCT ATGACAGCGA CTCGCGCACC
    ACCAATGGAT CACATGAGGA GGAAGGAGGA CAAACCGGAG ATAATAGCGT ATCAAGTGAC
    GTGACGTCTG AAGAACAGGA AGACATGATT TCAGTACAGG ATGCAGAAAT CACCCTCCTC
    AAGTCAGGAG AGCTCAGTGC CTCTGTGCCT CTGGATATAG ATGAGATTCC TCATTTCGGC
    GATGGCTCCC GTGAGCTGTT TGTCAAGTCC AGTTGTTACA GCACCATTCC CATCACAGTG
    GGCGACCGCG GACCCATCAT CAGCTGGGTT TTCTCCTCCG AGCCAAAGAG CATCTCCTTC
    ACTGTGGTGT ACAGAGAGAC ACTTGACACA CCTGTCGAGC AGGCCAAGGT TCTGATTCCC
    CTCACGCGCT GTAACTCACA CAAAGAGACC ATACAGGGAC AACTCAAAGT GCGCAACGCT
    GGAGTCTACA CACTCATCTT CGATAACTCA TTCTCCAGAT TTCTCTCCAA GAAGGTCAAC
    TACCACCTGA CCATTGAGAA GCCCGTCATC TACGATGGAA GTGACTTCCC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295366.1
    CDS83..4576
    Translation

    Target ORF information:

    RefSeq Version XM_009297091.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297091.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCCACTG GTGCTGCAGT CGGGGACAGT CAGATCCAGA GGATTATCAG AGATCTGCGC 
    GATGCTGTTT CCGAACTGAC AAAGGAGTAT AAAGAGAGCG GTGAGCCCAT TACTGATGAC
    AGCTCCAATC TGCACAAGTT CTCCTACAAG CTGGAGTATC TGCTTCAGTT CGACCAAAAG
    GAGAAAACAA CATTTCTGGG CTCCAGAAAA GACTACTGGG ATTACTTCAG CGACTGCCTG
    GCCAAAATCA AAGGAGCAAA TGATGGAATT CGCTTCGTTA AATCAATTTC AGAGCTGAAG
    ACGTCTCTGG GCAAGGGCAG AGCATTTATC CGTTACTCTC TGGTGCATCA GCGGCTGGCA
    GACACACTCC AGCAGTGCCT CATGAACTCC AGAGTCACCA GTGACTGGTA TAATCCGAGG
    AGTCCGTTTC TCAAACCCCA TCTCAGCGTG GACATCATTA GTTATCTCTA CGAGCTCAAT
    GATGTCCAGT TTGATGTGGC ATCCAGAGGT CATGACCTTG ACGCAGAATG GCCCACTTTT
    GCCAGGAGGA CATTAGGAAT GACTAGCTCT CCCAGTCACT TGTGGAAAGC ACCGAGTCGT
    AGTTCCAGCA TCAACAGTCT GGCCAGCACA TACTCACAGC AGCACCATGA GTTCCCTGGG
    AGTCCAGACT TTGGACCTGG GCTTTTATCA GATATGTCTA TGCAAAACTC CAGCATACTG
    AATGATACCT CAATGAGTGC TATAGACGAA CTCCGTCTGG AGCTTGACCA GTCTGAATTA
    AAGCAAAGAG AGCTTATTGA CCGGATTCAG CAGTTAGGTG ATGAGGGGAG TGAACTGCGT
    GGAGTTGTTG TGGAACTGCA AAGGCAGCTA GATGTTTCTT TAGCGGCCCA AGGTAACCAT
    CAGGAGCTAC AACGTAACCT GGAGGTCTTA ATTGAGAGCG AACATGCACT CTCCCGTGAG
    GTGGAGGTCT TAAGAGATCG AGAAACCAGG AGGGAGGTTT CCCATAAAGA CCTTCAGGAC
    ATGCTGGCAG CAGCGGAGCG TAAAAACGAG GAGCTGATGA CAAGGCTAGA TGGAGTATTG
    GATGAGAAAG GTCAAAGGGC GGCCAGTGAT TTCAACTCAG CCCAGAAGAT TCACGAGCTT
    TTGAATGAAT TAAAAGAGGC TGAGAAGAAG AGGATGGATG CTCTGGCTGA AGGAGAAGAG
    AAGAGGAGGC ATGCTGAACA TCTTGCCGAG GAAGTCAAAG TGAAAGATGA AGCTCTCAAA
    GAGGCTGAGG TGAAAATGGC AGCTTGGATG GAGAAGGGTG AACAACTGCA AACCAGGGCG
    GTGGAGCAGC GTAATTTCAT GGAGAAACTT CAAGGTGCAC TTGCAGTAAG AGAGAAAGAG
    ACCAGCAACC TGCAGAGGCA ACTACGAGAT CTTCAGAACT CTCTGGAGAA CATGGAAAAG
    CAGGCCAATG TGGAGAAGAA GAGGATGCAA GATGATAAAG AAGAGCTTGA GATGAAAATG
    AATGGTCTAG AGGGGCTGTT ACAATCACTT CGGACACAGT TAAAGGTTAA AGAATCTGAT
    CTTCTCTCCA GTACCAAGAG AGTTCATTTC CTGGAGAGGG AATCAGAGAA GCTTAGGAGC
    GAGAATCAGA AGTTGGAGTA TGAGTTGGAG AACAGCACCA AAAAGGAGGC CAAGAAGATT
    GATGAATACA AGGATAGCTG CGCCAAGCTT ATTGAGCAAA ACACTAAGCT TCTGCAGACG
    GTGAATAAGA ATGAAGAGAG CAAGAAAGAG TTGCTTGAAA ACAAATCATC CTTGGAGAGC
    GAGTTGGCGG GATTGAGGGC CTCAGAGAAG CAGTTACGAG CTCAAATCGA TGATGCCAAA
    GTGACTGTGG ATGAACGAGA GCAGCGGCTG AGAGAGGAGA ACAGAAATCT GGATGAGAGT
    CTGCAGAAGG CCAACATGCA GCTGGAGGAA TCCGAGAGCT CAATTCGGCA AAAGGAGCAG
    GAGAACAAGG ACCTCATGGA GGTTCAGGTC ACTCTTAAAT CAGCTCTCGC AGCCATGCAG
    AAAGAGATAC GTGACATAAA CAACCAGATC GGTGAACTTG AGAAGAATCT TGGAGTTGCG
    AGGTGTAACG AAGCAAATCT GAACGCGCAG CTTAAAGATA AGGCCACCCA GCTGGAAGAT
    CGAGAGAAGC TCTGTGAGGA GCTCCAGGGA AGAGTGGAAG AACTGGAAAG TCGGCAGAGG
    GATTTGGAAG TGGAGAAGAC AAAAGCAGAA AGAGCCTTTG TTAAACAAAC AGAGATGATT
    CAGAGTCTTG AAGCACAGAG AAACCTGGCA GAGAAGACAC AACTTGAAAA AAGCACATGT
    CAAGCTAAAG AAACTAAAGA GATGGCCTTA AAATTGACTC TTCTAGAAGA TCAACTGGGA
    TTGAGTGCGA AGGAAGTTTC TAAGCTACAA GAGGAAGTGG TTAATCTCAG GGCTAAACTT
    CACAGTGCTG TGGAGGAGAA GGACAAGACG CAAGCAAAGC TTGAGGTCAC CGAAGCTTCC
    TGTGCTGAAC TGCGGATCTT GACTGAGCAC TTGAAGAAGC AGGCCGAGGA GCAGAATCGG
    CTGCATGTGA GCGAGCTGCT GCAGTCTAGT GAACATGTTG ATAAATTAAC TTCGCAGCTG
    AACCAGGAGA CCTCAGCGCA TGAGAAAACC ACTGCAGCAC TTGCTTCCGC TAAAGAGGAT
    CTAGTGGCTC TCAAGGCCCA GAATGAGCGG ATGGTCCTGG AGAATGCGGA AACCAGAGAG
    AGTCTCCACC GGGTCAACAC TGAAATGGCA GAGTTGGGAA TGACTATTTG CAAGCTCACC
    GCCGAGCGGG AAGAGGCCCG GGAACGTTGG GCCGCTGAAG CTGTACGCAT CCAAGAACTT
    CAGCAGCACG GAGTGAAGGA GACAGAGCGA CTCAATGCCA GCTTGGTGGC TCTGCATCAA
    GAAAACTCCA GTCTGCAGGA AGAGCTCCAG CAAACAGACA AACTGTCAGA AACCATGCTG
    GAGCTAAAGC AGCTGCTGGA CAAAACTGAG GGCGAACGGG ACGCTGCACG GGAAGAGATC
    ACCGCTGTGA AATTCCAGAT GAGCACAGAG AGCATGTCTC TCAAGCACCA AATGAAGAGT
    CTTCAAGAAG AGATTGACGG TTTGAAAGAT CAGCTAGACA CAGAGAGGAA GAAGAAATCA
    GAGCTGGAGG CCAAATTATC CGAACTAGAG GGAGCGAATG TGGAATACTC TCGTCTGATT
    GAGGAGAAGG ACTCGCACAT TACTTACTGT GAGACTTTGC TGCGTGAGAG CGAGTCTGAG
    ACACAGCAAC TGCAGGAAAG AGCATCAAGA TCCAAAGAGG CTCTTAGTGA TGTAGAGAAG
    GAGAGGGAAG AATTGAAGCA GAAATTGGAT CAAGTGTTGA TGGAGACTCA GAACCAGCAT
    CTCCGCATGT CTGCTGAACT GGAGGACCTG GGACAAACTA AAGTCAACCT GGAGGAACGG
    CTCATTGAAC TTATTAGAGA TAAGGACGCT CTGTGGCAGA AGTCTGATGC TCTAGAGTTC
    GAGCAGAAGC TAAGAGCAGA GGAGCAGTGG TGGCTGGTGG ATAAAGAGGC CACACACTGT
    CTGGGCTGCC AGGGTCAGTT CACATGGTGG CTGCGGAGGC ATCACTGCAG GTTGTGTGGC
    CGTATCTTCT GCTACTATTG CAGCAACAAT TACGTGATGA CCAAAAACTC AAAGAAGGAG
    CGCTGCTGTC GAGAGTGTTA CACACAACAC GGCGCTGTGG TGGAGAGATT CACCAAAGCT
    GAACTCAACA GCTCATCAGA CAACCAGCCA TCAAACACTG CCGCTCAGCT GCCACCGCCT
    CCATATATAC CCACACCAAG AGTTACAGTT ACAGATCCTG GTGAGAGGGC TGATGATGGA
    GCGTTTGACA TTATTACAGA CGAGGAAGTG AACCAGATCT ATGACAGCGA CTCGCGCACC
    ACCAATGGAT CACATGAGGA GGAAGGAGGA CAAACCGGAG ATAATAGCGT ATCAAGTGAC
    GTGACGTCTG AAGAACAGGA AGACATGATT TCAGTACAGG ATGCAGAAAT CACCCTCCTC
    AAGTCAGGAG AGCTCAGTGC CTCTGTGCCT CTGGATATAG ATGAGATTCC TCATTTCGGC
    GATGGCTCCC GTGAGCTGTT TGTCAAGTCC AGTTGTTACA GCACCATTCC CATCACAGTG
    GGCGACCGCG GACCCATCAT CAGCTGGGTT TTCTCCTCCG AGCCAAAGAG CATCTCCTTC
    ACTGTGGTGT ACAGAGAGAC ACTTGACACA CCTGTCGAGC AGGCCAAGGT TCTGATTCCC
    CTCACGCGCT GTAACTCACA CAAAGAGACC ATACAGGGAC AACTCAAAGT GCGCAACGCT
    GGAGTCTACA CACTCATCTT CGATAACTCA TTCTCCAGAT TTCTCTCCAA GAAGGTCAAC
    TACCACCTGA CCATTGAGAA GCCCGTCATC TACGATGGAA GTGACTTCCC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.