col11a1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	col11a1a
Entrez Gene ID	565402
Full Name	collagen, type XI, alpha 1a
Synonyms	wu:fb34e11,wu:fc11b03,zgc:158335
General protein information	Preferred Names collagen, type XI, alpha 1a Names collagen alpha-1(XI) chain col11a1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021470209.1	XP_021325884.1	collagen alpha-1(XI) chain isoform X5
XM_017353843.1	XP_017209332.1	collagen alpha-1(XI) chain isoform X4
XM_005162813.1	XP_005162870.1	collagen alpha-1(XI) chain isoform X1
XM_005162818.1	XP_005162875.1	collagen alpha-1(XI) chain isoform X4
XM_021470210.1	XP_021325885.1	collagen alpha-1(XI) chain isoform X6
XM_005162814.1	XP_005162871.1	collagen alpha-1(XI) chain isoform X2
XM_005162815.1	XP_005162872.1	collagen alpha-1(XI) chain isoform X3
NM_001083844.1	NP_001077313.1	collagen alpha-1(XI) chain

Pathways from BioSystems

Homologies

Bos taurus (cattle)	COL11A1	NP_001159981.1
Mus musculus (house mouse)	Col11a1	NP_031755.2
Danio rerio (zebrafish)	col11a1a	NP_001077313.1
Xenopus tropicalis (tropical clawed frog)	col5a3	XP_004913770.1
Rattus norvegicus (Norway rat)	Col11a1	NP_037249.1
Gallus gallus (chicken)	COL11A1	XP_001231624.2
Pan troglodytes (chimpanzee)	COL11A1	XP_001139974.1
	LOC712753	XP_002808276.1
Canis lupus familiaris (dog)	COL11A1	XP_005621909.1
Homo sapiens (human)	COL11A1	NP_542196.2

Gene Ontology
Bibliography

Related articles in PubMed

Molecular Mechanisms of Developmental Toxicity Induced by Graphene Oxide at Predicted Environmental Concentrations.
Zhang X, Zhou Q, Zou W, Hu X
Environmental science & technology51(14)7861-7871(2017 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following col11a1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col11a1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00692	NM_001083844.1 Latest version!	Danio rerio collagen, type XI, alpha 1a (col11a1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa151720	XM_021470209.1 Latest version!	Danio rerio collagen, type XI, alpha 1a (col11a1a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa151721	XM_021470210.1 Latest version!	Danio rerio collagen, type XI, alpha 1a (col11a1a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa28707	XM_005162813.1 Latest version!	Danio rerio collagen, type XI, alpha 1a (col11a1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa28708	XM_005162814.1 Latest version!	Danio rerio collagen, type XI, alpha 1a (col11a1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa28709	XM_005162815.1 Latest version!	Danio rerio collagen, type XI, alpha 1a (col11a1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa28712	XM_005162818.1 Latest version!	Danio rerio collagen, type XI, alpha 1a (col11a1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa57601	XM_017353843.1 Latest version!	Danio rerio collagen, type XI, alpha 1a (col11a1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

col11a1a ( NM_001083844.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001077313.1 Danio rerio collagen, type XI, alpha 1a (col11a1a), mRNA.

Price & Availability↑

CloneID	ODa00692
Clone ID Related Accession (Same CDS sequence)	NM_001083844.1
Accession Version	NM_001083844.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-23
Organism	Danio rerio(zebrafish)
Product	collagen alpha-1(XI) chain
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC133083.1. On Apr 5, 2007 this sequence version replaced XM_688682.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC133083.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAGAGGC CAAGGTGGTC CCCAATGTGG AAAACGAAAA GGTGGCTTTT GGATTCCACT 
GTTTTCAAAT TGATCACAGT TTCCCTCGTC CTGGTTCTGC AGGCTCCACA TGTCAGAGCA 
GAACCCGTGG ATGTCTTAAA AGTATTAGAA TTTCCCACTG TCCCAGAAGG AGTAGAGAAA 
ACGTCAGGAT TCTGCACAAA CCGAAGAGCA TCACAGCCAG ACGCGGCCTA CAAAATCAGC 
AAGAATGCCC AGCTCAGTGC CCCGACCAAG CAGCTTTTCC CAGATGGCGT TTTCCCTGAA 
GACTTCTCCA TCCTAACCAC GATCAAGCCC AAAGCAGGGA TCCAGTCCTT CCTCCTGTCC 
ATCTACAATG AGAAAGGCGT CCAGCAGCTG GGCGTGGAGG TGGGCCGTTC ACCTGTCTTC 
CTGTACGAGG ATCAGGAGGG CAAACCTGCC CCGGAGGATT ACCCTCTGTT CCGCGCTCTC 
AATCTGGCCG ACGGAAAGTG GCATCGCGTA GGCATCAGCG TTGAGAAGAA GACAGTGACT 
ATTGTTGTCG ACTGTAAGAA GAAGCTGACC AAGCCATTGG GCAGGAGTGA CCATGCCAGT 
ATCGACACCA AGGGTATCAC CGTTTTTGGC ACCAGGATCC TGGATGAGGA ATCTTTTGAG 
GGTGATATAC AACAACTGCT GATTGTGGCT GACCCTAAAG CAGCTTACGA CTACTGTGAA 
CATTACAGCC CAGACTGTGA CGTCCCTCAC AAGGACACAC TGCAGGCACA GGAGCCCGGA 
GAGGAGTACA CAACTCCTGT TGAATATGGT GATTTATATG ATTATTACGA GGGAGGCACA 
CCCACAGCGA CTGATGAGTT TTCTGAGCCG CAGAGAGTGA AACGTAGTGT CTCCATGAAA 
TCCAAGAGGA CAAAAAAGTC CCCTAAATCT TTTGCCAAAC CCTATAAGTA CCTGGAGCCA 
AAACAGGCTA AAAAGTTAGT GCCTAAGAAG AAGAGATACG GATACCTGCC ACCAACACCA 
AGACAAGCCA TTCTAGGGCT GGGTGGAGGT GCTGCTCTTG GTCAGAAAGG TGAGAAAGGA 
GAGCCTGCAG TGATTGAACC AGGAATGCTG ATCGAGGGAC CTTCAGGACC ACCTGGACCT 
GCTGGTCTTC CAGGTCCTCC AGGTTTACAA GGCCCCCCTG GTTTGGCTGG AGATCCTGGT 
GATAGGGGTC CTCCAGGCCG TGCTGGTCTT CCTGGAGCTG ATGGTTTGCC AGGACCACCT 
GGAACTATGC TCATGCTGCC GTTTCGTTTT GGTGGTGATG GAGAAAAGGG CCCAGTGGTT 
TCAGCTCAGG AGGCTCAGGC TCAGGCTATT CTCTCTCAGG CCAGGCTCGC CATGAGAGGT 
CCACCGGGTC CAATGGGTCT CACTGGACGA TCTGGACCAG TGGGTCTTCC AGGTCCTCCT 
GGACTGAAGG GAGAAAGTGG GGATCATGGT CCTCAGGGTC CAAGGGGAAT CCAAGGCCCA 
CCAGGGCAAA TAGGAAAACC TGGAAAGAGG GGTCGTGCTG GAGCTGATGG TGCCCGAGGA 
ATGCCTGGAG AGTCTGGATC TAAGGGTGAC AGAGGTTTCG ATGGGCTTCC GGGTCTGCCT 
GGTGAGAAAG GACACAGGGG TGAGACCGGC CCTGCTGGAC CCCCGGGACC AACTGGAGAG 
GATGGACCAA GAGGAGAAGA TGGTGAGATC GGACCAAGAG GACTTGCTGG TGAGAGCGGG 
CCGCGAGGTC TTCTCGGCCC AAGAGGCCCT CCTGGACCTC CTGGACAACC TGGCATTGCT 
GGTGTTGATG GTCCGCAGGG TCCCAAAGGA AACATGGGGC CTCAGGGAGA GCCAGGTCCT 
CCTGGACAGC AGGGAATCCC AGGAGGACAG GGTCTTCCAG GTCCACAGGG ACCCATCGGA 
CCACCCGGAG AAAAAGGCCC TCAGGGAAGG TCTGGTTTGC CTGGATTGCC TGGTGCAGAT 
GGGCCTCCTG GACATCCTGG TAAAGAGGGT CCACCTGGAG AGAAAGGAGC TTTGGGCCAG 
CCTGGTCCCC AGGGCCCCAT CGGCTATCCT GGCCCTCGCG GTGTGAAGGG TGCAGATGGT 
GTCCGTGGTC TAAAGGGAGG CAAAGGAGAG AAGGGTGAGG ATGGATTCCC AGGTTTCAAG 
GGAGACATGG GTCTCAAAGG CGACAGGGGA GAGCTGGGAC TGGCTGGACC CAGAGGAGAA 
GATGGTCCAG AGGGCCCGAA GGGTCGTGCA GGGCCTAACG GTGAATCTGG ACCCCAGGGA 
TCTGCCGGAG AAAAGGGCAA ACTAGGTGTT CCAGGATTGC CCGGCTATCC AGGACGTCAA 
GGGCCAAAGG GTTCGACCGG TTTCCCCGGA TTCCCAGGAG CCAATGGAGA GAAAGGAGGC 
AGGGGGGTGG CTGGGAAAGC TGGTCCAAGA GGCCAAAGAG GTCCAACGGG CCCCCGTGGT 
GCACGAGGTG CAAGAGGCCC AACAGGAAAG CCAGGCCCGA AGGGAACAGC AGGCAACGAC 
GGTCCACCTG GCCCACCCGG TGAGAGAGGC CCTCAAGGAC CCCAGGGTCC TGTTGGTTTC 
CCAGGACCAA AGGGTCCCCC TGGACCACCT GGAAAGGATG GGCTGCCCGG TCACCCTGGC 
CAGCGGGGAG AGACTGGATT CCAAGGCAAA ACTGGCCCAC CAGGACCTGG AGGTGTTGTT 
GGACCACAAG GACCGACTGG AGAAACCGGT CCAATTGGTG AAAGAGGACA TCCTGGACCC 
CCTGGTCCCC CCGGAGAGCA GGGTCTTCCT GGCGCTGGTG GAAAAGAAGG GGCAAAGGGA 
GATCCAGGTC CTCAGGGCAG TCCAGGAAAA GACGGTCCAC CAGGACTCAG AGGGTTCCCT 
GGAGAAAGAG GTCTTCCAGG AGTGACGGGT CCACCTGGTT TGAAAGGCGC CGAGGGCCCT 
CAGGGCCCTC CTGGCCCTGT TGGTTCCCCT GGTGAGAGAG GAGCAGCAGG ATCTGCTGGT 
CCCATTGGCC TGCCTGGACG TACTGGACCT CAGGGACCCC CAGGACCTGC TGGAGAGAAG 
GGTGCACCGG GAGAGAGAGG TCCTCTGGGT CCAGCCGGTA GGGATGGAGT TCAAGGTCCA 
GTTGGTCTGC CAGGTCCAGC AGGACCTCAG GGTCCACCTG GAGAAGATGG TGACAAGGGA 
GAAGTGGGTG AACCAGGCCA GAAGGGAAGC AAAGCTGACA AAGGAGAACA GGGTCCACCA 
GGTCCTCCTG GTCTTCAGGG ACCCATTGGA GCTCCTGGAC CTGCTGGAGC AGATGGCGAG 
CCCGGTCCTC GTGGACAGCA GGGTATGTTT GGACAGAAGG GAGATGAAGG ATCCCGGGGC 
TTCCCTGGAC CTCCTGGTCC CATCGGTCTG CAGGGTCTTC CAGGACCACC TGGCGAGAAA 
GGTGAAAATG GAGATGTTGG TCCTATGGGT CCTCCTGGTC CACCAGGTCC CAGAGGACCT 
CAGGGTCCTC CTGGTGCTGA TGGCCCGCAA GGTCCTCCTG GTGGAATAGG AGGAATGGGA 
GCCGTGGGTG AGAAGGGTGA GCAAGGAGAA GCTGGCAACC CTGGACCTCC TGGAGAGCCT 
GGACCTGGGG GCCCGAAAGG TGAGAGAGGA GAGAAAGGTG AAGCTGGTCC CCCTGGTGCT 
GCTGGACCTG CAGGTCCCAA AGGACCCCCT GGAGATGATG GACCCAAGGG TAACCCTGGC 
CCTGTTGGTT TTCCTGGAGA CCCAGGTCCA CCTGGTGAGC CAGGTGTTGC TGGAATAGAT 
GGAGGCCCAG GAGAGAAGGG AGAGGATGGA GAGTCTGGTC AGCCTGGACC CCCTGGTCCT 
TCTGGAGAAG CTGGTCCACC AGGACCTCCT GGAAAACGAG GACCCCCGGG GGCGCATGGA 
GCTGAAGGCA GACAAGGAGA GAAGGGCGCC AAGGGAGAAG CAGGACTTGA GGGTCCACCA 
GGTAAAACTG GACCAGTTGG TCCTCAAGGA CCCTCTGGAA AGCCTGGTCC TGAAGGTCTC 
AGAGGAATCC CCGGTCCAGT CGGTGAACAA GGACTTCCTG GTGCTCCTGG ACAAGATGGT 
CCACCTGGAC CAATGGGACC TCCTGGTCTG CCTGGCCTTA AAGGAGACCC TGGCTCTAAG 
GGTGAGAAGG GTCACCCTGG TCTTATCGGT CTCATCGGGC CGCCCGGTGA ACAGGGAGAG 
AAGGGAGATC GGGGTTTGCC TGGACCTCAG GGTGCTGCCG GCAGTAAGGG TGATAATGGC 
ATCAGTGGTG CTTCTGGTCC AATTGGTCCT CCTGGTCCTC CTGGATTGCC TGGTCCACAA 
GGGCCAAAGG GATCCAAGGG TTCAACTGGT CCTGCTGGTC AAAAAGGAGA CACTGGCACG 
GTTGGACCTC CTGGGCCTCC TGGTCCTCCT GGTGAGGTCA TCCAGCCTCT GCCCATCCAC 
AGTCCCAAGA AATCCCGTCG CTCCATCGAC ATGCAGGCGG ATGAAGCAGG CACCATGCTG 
GACTATGGAG AGGGCATGGA GGACATATTC AGCTCTCTCA ATAATCTCAA ACAGGACATC 
GAGCGCATGA AGTACCCCAT GGGCACGCAA AACAACCCAG CACGAAGCTG TAAAGACCTT 
CAGCTCGCTC ACCCTGAATT CCCAGATGGC GAATATTACA TTGATCCTAA CCAGGGCTGC 
TCTGGAGACT CCTTCAAAGT GTACTGCAAC TTCACAGCAG GAGGAGAGAC CTGCATCTTC 
CCTGATAAAA AGTCAAATGG AGTGAGAATA TCTTCATGGC CCAAAGAGGT GCCCGGCTCC 
TGGTTCAGTG AATTTAAGCG TGGCAAAATA CTTTCATACG TGGACGCAGA AGGCAACTCG 
ATAAACATGG TCCAGATGAC GTTCCTCAAA CTACTGACAG CCTCCGCCAG GCAAAACCTG 
ACCTACAGCT GCCACCAGTC AGTGGCCTGG CACGACGCCA CTACGGACAG CTATGACAGA 
GCATTACACT TCCTCGGCTC CAACGACGAG GAGATCTCAT ACGACAACAA CCCCTACATC 
AAGGCCCTGT CAGACGGCTG TGCGGTGAGG AAGGGCTATG GAAAGACAGT ACTGGAGATT 
AACACTCCCA AAATTGATCA GGTTCCAATC GTCGACGTGA AGTTCACTGA CTTTGGGGAT 
CCCAACCAGA AATTTGGATT TGAAGTTGGT CCAGTCTGCT TCCTTGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077313.1
CDS	189..5399
Translation	MERPRWSPMWKTKRWLLDSTVFKLITVSLVLVLQAPHVRAEPVDVLKVLEFPTVPEGVEKTSGFCTNRRASQPDAAYKISKNAQLSAPTKQLFPDGVFPEDFSILTTIKPKAGIQSFLLSIYNEKGVQQLGVEVGRSPVFLYEDQEGKPAPEDYPLFRALNLADGKWHRVGISVEKKTVTIVVDCKKKLTKPLGRSDHASIDTKGITVFGTRILDEESFEGDIQQLLIVADPKAAYDYCEHYSPDCDVPHKDTLQAQEPGEEYTTPVEYGDLYDYYEGGTPTATDEFSEPQRVKRSVSMKSKRTKKSPKSFAKPYKYLEPKQAKKLVPKKKRYGYLPPTPRQAILGLGGGAALGQKGEKGEPAVIEPGMLIEGPSGPPGPAGLPGPPGLQGPPGLAGDPGDRGPPGRAGLPGADGLPGPPGTMLMLPFRFGGDGEKGPVVSAQEAQAQAILSQARLAMRGPPGPMGLTGRSGPVGLPGPPGLKGESGDHGPQGPRGIQGPPGQIGKPGKRGRAGADGARGMPGESGSKGDRGFDGLPGLPGEKGHRGETGPAGPPGPTGEDGPRGEDGEIGPRGLAGESGPRGLLGPRGPPGPPGQPGIAGVDGPQGPKGNMGPQGEPGPPGQQGIPGGQGLPGPQGPIGPPGEKGPQGRSGLPGLPGADGPPGHPGKEGPPGEKGALGQPGPQGPIGYPGPRGVKGADGVRGLKGGKGEKGEDGFPGFKGDMGLKGDRGELGLAGPRGEDGPEGPKGRAGPNGESGPQGSAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGGRGVAGKAGPRGQRGPTGPRGARGARGPTGKPGPKGTAGNDGPPGPPGERGPQGPQGPVGFPGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPGGVVGPQGPTGETGPIGERGHPGPPGPPGEQGLPGAGGKEGAKGDPGPQGSPGKDGPPGLRGFPGERGLPGVTGPPGLKGAEGPQGPPGPVGSPGERGAAGSAGPIGLPGRTGPQGPPGPAGEKGAPGERGPLGPAGRDGVQGPVGLPGPAGPQGPPGEDGDKGEVGEPGQKGSKADKGEQGPPGPPGLQGPIGAPGPAGADGEPGPRGQQGMFGQKGDEGSRGFPGPPGPIGLQGLPGPPGEKGENGDVGPMGPPGPPGPRGPQGPPGADGPQGPPGGIGGMGAVGEKGEQGEAGNPGPPGEPGPGGPKGERGEKGEAGPPGAAGPAGPKGPPGDDGPKGNPGPVGFPGDPGPPGEPGVAGIDGGPGEKGEDGESGQPGPPGPSGEAGPPGPPGKRGPPGAHGAEGRQGEKGAKGEAGLEGPPGKTGPVGPQGPSGKPGPEGLRGIPGPVGEQGLPGAPGQDGPPGPMGPPGLPGLKGDPGSKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGAAGSKGDNGISGASGPIGPPGPPGLPGPQGPKGSKGSTGPAGQKGDTGTVGPPGPPGPPGEVIQPLPIHSPKKSRRSIDMQADEAGTMLDYGEGMEDIFSSLNNLKQDIERMKYPMGTQNNPARSCKDLQLAHPEFPDGEYYIDPNQGCSGDSFKVYCNFTAGGETCIFPDKKSNGVRISSWPKEVPGSWFSEFKRGKILSYVDAEGNSINMVQMTFLKLLTASARQNLTYSCHQSVAWHDATTDSYDRALHFLGSNDEEISYDNNPYIKALSDGCAVRKGYGKTVLEINTPKIDQVPIVDVKFTDFGDPNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	NM_001083844.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XI, alpha 1a (col11a1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083844.1

ORF Insert Sequence:

ATGGAGAGGC CAAGGTGGTC CCCAATGTGG AAAACGAAAA GGTGGCTTTT GGATTCCACT 
GTTTTCAAAT TGATCACAGT TTCCCTCGTC CTGGTTCTGC AGGCTCCACA TGTCAGAGCA 
GAACCCGTGG ATGTCTTAAA AGTATTAGAA TTTCCCACTG TCCCAGAAGG AGTAGAGAAA 
ACGTCAGGAT TCTGCACAAA CCGAAGAGCA TCACAGCCAG ACGCGGCCTA CAAAATCAGC 
AAGAATGCCC AGCTCAGTGC CCCGACCAAG CAGCTTTTCC CAGATGGCGT TTTCCCTGAA 
GACTTCTCCA TCCTAACCAC GATCAAGCCC AAAGCAGGGA TCCAGTCCTT CCTCCTGTCC 
ATCTACAATG AGAAAGGCGT CCAGCAGCTG GGCGTGGAGG TGGGCCGTTC ACCTGTCTTC 
CTGTACGAGG ATCAGGAGGG CAAACCTGCC CCGGAGGATT ACCCTCTGTT CCGCGCTCTC 
AATCTGGCCG ACGGAAAGTG GCATCGCGTA GGCATCAGCG TTGAGAAGAA GACAGTGACT 
ATTGTTGTCG ACTGTAAGAA GAAGCTGACC AAGCCATTGG GCAGGAGTGA CCATGCCAGT 
ATCGACACCA AGGGTATCAC CGTTTTTGGC ACCAGGATCC TGGATGAGGA ATCTTTTGAG 
GGTGATATAC AACAACTGCT GATTGTGGCT GACCCTAAAG CAGCTTACGA CTACTGTGAA 
CATTACAGCC CAGACTGTGA CGTCCCTCAC AAGGACACAC TGCAGGCACA GGAGCCCGGA 
GAGGAGTACA CAACTCCTGT TGAATATGGT GATTTATATG ATTATTACGA GGGAGGCACA 
CCCACAGCGA CTGATGAGTT TTCTGAGCCG CAGAGAGTGA AACGTAGTGT CTCCATGAAA 
TCCAAGAGGA CAAAAAAGTC CCCTAAATCT TTTGCCAAAC CCTATAAGTA CCTGGAGCCA 
AAACAGGCTA AAAAGTTAGT GCCTAAGAAG AAGAGATACG GATACCTGCC ACCAACACCA 
AGACAAGCCA TTCTAGGGCT GGGTGGAGGT GCTGCTCTTG GTCAGAAAGG TGAGAAAGGA 
GAGCCTGCAG TGATTGAACC AGGAATGCTG ATCGAGGGAC CTTCAGGACC ACCTGGACCT 
GCTGGTCTTC CAGGTCCTCC AGGTTTACAA GGCCCCCCTG GTTTGGCTGG AGATCCTGGT 
GATAGGGGTC CTCCAGGCCG TGCTGGTCTT CCTGGAGCTG ATGGTTTGCC AGGACCACCT 
GGAACTATGC TCATGCTGCC GTTTCGTTTT GGTGGTGATG GAGAAAAGGG CCCAGTGGTT 
TCAGCTCAGG AGGCTCAGGC TCAGGCTATT CTCTCTCAGG CCAGGCTCGC CATGAGAGGT 
CCACCGGGTC CAATGGGTCT CACTGGACGA TCTGGACCAG TGGGTCTTCC AGGTCCTCCT 
GGACTGAAGG GAGAAAGTGG GGATCATGGT CCTCAGGGTC CAAGGGGAAT CCAAGGCCCA 
CCAGGGCAAA TAGGAAAACC TGGAAAGAGG GGTCGTGCTG GAGCTGATGG TGCCCGAGGA 
ATGCCTGGAG AGTCTGGATC TAAGGGTGAC AGAGGTTTCG ATGGGCTTCC GGGTCTGCCT 
GGTGAGAAAG GACACAGGGG TGAGACCGGC CCTGCTGGAC CCCCGGGACC AACTGGAGAG 
GATGGACCAA GAGGAGAAGA TGGTGAGATC GGACCAAGAG GACTTGCTGG TGAGAGCGGG 
CCGCGAGGTC TTCTCGGCCC AAGAGGCCCT CCTGGACCTC CTGGACAACC TGGCATTGCT 
GGTGTTGATG GTCCGCAGGG TCCCAAAGGA AACATGGGGC CTCAGGGAGA GCCAGGTCCT 
CCTGGACAGC AGGGAATCCC AGGAGGACAG GGTCTTCCAG GTCCACAGGG ACCCATCGGA 
CCACCCGGAG AAAAAGGCCC TCAGGGAAGG TCTGGTTTGC CTGGATTGCC TGGTGCAGAT 
GGGCCTCCTG GACATCCTGG TAAAGAGGGT CCACCTGGAG AGAAAGGAGC TTTGGGCCAG 
CCTGGTCCCC AGGGCCCCAT CGGCTATCCT GGCCCTCGCG GTGTGAAGGG TGCAGATGGT 
GTCCGTGGTC TAAAGGGAGG CAAAGGAGAG AAGGGTGAGG ATGGATTCCC AGGTTTCAAG 
GGAGACATGG GTCTCAAAGG CGACAGGGGA GAGCTGGGAC TGGCTGGACC CAGAGGAGAA 
GATGGTCCAG AGGGCCCGAA GGGTCGTGCA GGGCCTAACG GTGAATCTGG ACCCCAGGGA 
TCTGCCGGAG AAAAGGGCAA ACTAGGTGTT CCAGGATTGC CCGGCTATCC AGGACGTCAA 
GGGCCAAAGG GTTCGACCGG TTTCCCCGGA TTCCCAGGAG CCAATGGAGA GAAAGGAGGC 
AGGGGGGTGG CTGGGAAAGC TGGTCCAAGA GGCCAAAGAG GTCCAACGGG CCCCCGTGGT 
GCACGAGGTG CAAGAGGCCC AACAGGAAAG CCAGGCCCGA AGGGAACAGC AGGCAACGAC 
GGTCCACCTG GCCCACCCGG TGAGAGAGGC CCTCAAGGAC CCCAGGGTCC TGTTGGTTTC 
CCAGGACCAA AGGGTCCCCC TGGACCACCT GGAAAGGATG GGCTGCCCGG TCACCCTGGC 
CAGCGGGGAG AGACTGGATT CCAAGGCAAA ACTGGCCCAC CAGGACCTGG AGGTGTTGTT 
GGACCACAAG GACCGACTGG AGAAACCGGT CCAATTGGTG AAAGAGGACA TCCTGGACCC 
CCTGGTCCCC CCGGAGAGCA GGGTCTTCCT GGCGCTGGTG GAAAAGAAGG GGCAAAGGGA 
GATCCAGGTC CTCAGGGCAG TCCAGGAAAA GACGGTCCAC CAGGACTCAG AGGGTTCCCT 
GGAGAAAGAG GTCTTCCAGG AGTGACGGGT CCACCTGGTT TGAAAGGCGC CGAGGGCCCT 
CAGGGCCCTC CTGGCCCTGT TGGTTCCCCT GGTGAGAGAG GAGCAGCAGG ATCTGCTGGT 
CCCATTGGCC TGCCTGGACG TACTGGACCT CAGGGACCCC CAGGACCTGC TGGAGAGAAG 
GGTGCACCGG GAGAGAGAGG TCCTCTGGGT CCAGCCGGTA GGGATGGAGT TCAAGGTCCA 
GTTGGTCTGC CAGGTCCAGC AGGACCTCAG GGTCCACCTG GAGAAGATGG TGACAAGGGA 
GAAGTGGGTG AACCAGGCCA GAAGGGAAGC AAAGCTGACA AAGGAGAACA GGGTCCACCA 
GGTCCTCCTG GTCTTCAGGG ACCCATTGGA GCTCCTGGAC CTGCTGGAGC AGATGGCGAG 
CCCGGTCCTC GTGGACAGCA GGGTATGTTT GGACAGAAGG GAGATGAAGG ATCCCGGGGC 
TTCCCTGGAC CTCCTGGTCC CATCGGTCTG CAGGGTCTTC CAGGACCACC TGGCGAGAAA 
GGTGAAAATG GAGATGTTGG TCCTATGGGT CCTCCTGGTC CACCAGGTCC CAGAGGACCT 
CAGGGTCCTC CTGGTGCTGA TGGCCCGCAA GGTCCTCCTG GTGGAATAGG AGGAATGGGA 
GCCGTGGGTG AGAAGGGTGA GCAAGGAGAA GCTGGCAACC CTGGACCTCC TGGAGAGCCT 
GGACCTGGGG GCCCGAAAGG TGAGAGAGGA GAGAAAGGTG AAGCTGGTCC CCCTGGTGCT 
GCTGGACCTG CAGGTCCCAA AGGACCCCCT GGAGATGATG GACCCAAGGG TAACCCTGGC 
CCTGTTGGTT TTCCTGGAGA CCCAGGTCCA CCTGGTGAGC CAGGTGTTGC TGGAATAGAT 
GGAGGCCCAG GAGAGAAGGG AGAGGATGGA GAGTCTGGTC AGCCTGGACC CCCTGGTCCT 
TCTGGAGAAG CTGGTCCACC AGGACCTCCT GGAAAACGAG GACCCCCGGG GGCGCATGGA 
GCTGAAGGCA GACAAGGAGA GAAGGGCGCC AAGGGAGAAG CAGGACTTGA GGGTCCACCA 
GGTAAAACTG GACCAGTTGG TCCTCAAGGA CCCTCTGGAA AGCCTGGTCC TGAAGGTCTC 
AGAGGAATCC CCGGTCCAGT CGGTGAACAA GGACTTCCTG GTGCTCCTGG ACAAGATGGT 
CCACCTGGAC CAATGGGACC TCCTGGTCTG CCTGGCCTTA AAGGAGACCC TGGCTCTAAG 
GGTGAGAAGG GTCACCCTGG TCTTATCGGT CTCATCGGGC CGCCCGGTGA ACAGGGAGAG 
AAGGGAGATC GGGGTTTGCC TGGACCTCAG GGTGCTGCCG GCAGTAAGGG TGATAATGGC 
ATCAGTGGTG CTTCTGGTCC AATTGGTCCT CCTGGTCCTC CTGGATTGCC TGGTCCACAA 
GGGCCAAAGG GATCCAAGGG TTCAACTGGT CCTGCTGGTC AAAAAGGAGA CACTGGCACG 
GTTGGACCTC CTGGGCCTCC TGGTCCTCCT GGTGAGGTCA TCCAGCCTCT GCCCATCCAC 
AGTCCCAAGA AATCCCGTCG CTCCATCGAC ATGCAGGCGG ATGAAGCAGG CACCATGCTG 
GACTATGGAG AGGGCATGGA GGACATATTC AGCTCTCTCA ATAATCTCAA ACAGGACATC 
GAGCGCATGA AGTACCCCAT GGGCACGCAA AACAACCCAG CACGAAGCTG TAAAGACCTT 
CAGCTCGCTC ACCCTGAATT CCCAGATGGC GAATATTACA TTGATCCTAA CCAGGGCTGC 
TCTGGAGACT CCTTCAAAGT GTACTGCAAC TTCACAGCAG GAGGAGAGAC CTGCATCTTC 
CCTGATAAAA AGTCAAATGG AGTGAGAATA TCTTCATGGC CCAAAGAGGT GCCCGGCTCC 
TGGTTCAGTG AATTTAAGCG TGGCAAAATA CTTTCATACG TGGACGCAGA AGGCAACTCG 
ATAAACATGG TCCAGATGAC GTTCCTCAAA CTACTGACAG CCTCCGCCAG GCAAAACCTG 
ACCTACAGCT GCCACCAGTC AGTGGCCTGG CACGACGCCA CTACGGACAG CTATGACAGA 
GCATTACACT TCCTCGGCTC CAACGACGAG GAGATCTCAT ACGACAACAA CCCCTACATC 
AAGGCCCTGT CAGACGGCTG TGCGGTGAGG AAGGGCTATG GAAAGACAGT ACTGGAGATT 
AACACTCCCA AAATTGATCA GGTTCCAATC GTCGACGTGA AGTTCACTGA CTTTGGGGAT 
CCCAACCAGA AATTTGGATT TGAAGTTGGT CCAGTCTGCT TCCTTGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col11a1a ( XM_021470209.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325884.1 Danio rerio collagen, type XI, alpha 1a (col11a1a), transcript variant X5, mRNA.

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CloneID	ODa151720
Clone ID Related Accession (Same CDS sequence)	XM_021470209.1
Accession Version	XM_021470209.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-1(XI) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGGC CAAGGTGGTC CCCAATGTGG AAAACGAAAA GGTGGCTTTT GGATTCCACT 
GTTTTCAAAT TGATCACAGT TTCCCTCGTC CTGGTTCTGC AGGCTCCACA TGTCAGAGCA 
GCAGAACCCG TGGATGTCTT AAAAGTATTA GAATTTCCCA CTGTCCCAGA AGGAGTAGAG 
AAAACGTCAG GATTCTGCAC AAACCGAAGA GCATCACAGC CAGACGCGGC CTACAAAATC 
AGCAAGAATG CCCAGCTCAG TGCCCCGACC AAGCAGCTTT TCCCAGATGG CGTTTTCCCT 
GAAGACTTCT CCATCCTAAC CACGATCAAG CCCAAAGCAG GGATCCAGTC CTTCCTCCTG 
TCCATCTACA ATGAGAAAGG CGTCCAGCAG CTGGGCGTGG AGGTGGGCCG TTCACCTGTC 
TTCCTGTACG AGGATCAGGA GGGCAAACCT GCCCCGGAGG ATTACCCTCT GTTCCGCGCT 
CTCAATCTGG CCGACGGAAA GTGGCATCGC GTAGGCATCA GCGTTGAGAA GAAGACAGTG 
ACTATTGTTG TCGACTGTAA GAAGAAGCTG ACCAAGCCAT TGGGCAGGAG TGACCATGCC 
AGTATCGACA CCAAGGGTAT CACCGTTTTT GGCACCAGGA TCCTGGATGA GGAATCTTTT 
GAGGGTGATA TACAACAACT GCTGATTGTG GCTGACCCTA AAGCAGCTTA CGACTACTGT 
GAACATTACA GCCCAGACTG TGACGTCCCT CACAAGGACA CACTGCAGGC ACAGGAGCCC 
GGAGAGGAGT CTAACAATGT AGATGGAGAG TACTATACAG ACAGTGAACT TGACTACGGA 
ACAGTAGATG CTTCCAAAAC GCCTGTCGCA ACCACTGGAC CAAACAAGCT GGGTGGAGGT 
GCTGCTCTTG GTCAGAAAGG TGAGAAAGGA GAGCCTGCAG TGATTGAACC AGGAATGCTG 
ATCGAGGGAC CTTCAGGACC ACCTGGACCT GCTGGTCTTC CAGGTCCTCC AGGTTTACAA 
GGCCCCCCTG GTTTGGCTGG AGATCCTGGT GATAGGGGTC CTCCAGGCCG TGCTGGTCTT 
CCTGGAGCTG ATGGTTTGCC AGGACCACCT GGAACTATGC TCATGCTGCC GTTTCGTTTT 
GGTGGTGATG GAGAAAAGGG CCCAGTGGTT TCAGCTCAGG AGGCTCAGGC TCAGGCTATT 
CTCTCTCAGG CCAGGCTCGC CATGAGAGGT CCACCGGGTC CAATGGGTCT CACTGGACGA 
TCTGGACCAG TGGGTCTTCC AGGTCCTCCT GGACTGAAGG GAGAAAGTGG GGATCATGGT 
CCTCAGGGTC CAAGGGGAAT CCAAGGCCCA CCAGGGCAAA TAGGAAAACC TGGAAAGAGG 
GGTCGTGCTG GAGCTGATGG TGCCCGAGGA ATGCCTGGAG AGTCTGGATC TAAGGGTGAC 
AGAGGTTTCG ATGGGCTTCC GGGTCTGCCT GGTGAGAAAG GACACAGGGG TGAGACCGGC 
CCTGCTGGAC CCCCGGGACC AACTGGAGAG GATGGACCAA GAGGAGAAGA TGGTGAGATC 
GGACCAAGAG GACTTGCTGG TGAGAGCGGG CCGCGAGGTC TTCTCGGCCC AAGAGGCCCT 
CCTGGACCTC CTGGACAACC TGGCATTGCT GGTGTTGATG GTCCGCAGGG TCCCAAAGGA 
AACATGGGGC CTCAGGGAGA GCCAGGTCCT CCTGGACAGC AGGGAATCCC AGGAGGACAG 
GGTCTTCCAG GTCCACAGGG ACCCATCGGA CCACCCGGAG AAAAAGGCCC TCAGGGAAGG 
TCTGGTTTGC CTGGATTGCC TGGTGCAGAT GGGCCTCCTG GACATCCTGG TAAAGAGGGT 
CCACCTGGAG AGAAAGGAGC TTTGGGCCAG CCTGGTCCCC AGGGCCCCAT CGGCTATCCT 
GGCCCTCGCG GTGTGAAGGG TGCAGATGGT GTCCGTGGTC TAAAGGGAGG CAAAGGAGAG 
AAGGGTGAGG ATGGATTCCC AGGTTTCAAG GGAGACATGG GTCTCAAAGG CGACAGGGGA 
GAGCTGGGAC TGGCTGGACC CAGAGGAGAA GATGGTCCAG AGGGCCCGAA GGGTCGTGCA 
GGGCCTAACG GTGAATCTGG ACCCCAGGGA TCTGCCGGAG AAAAGGGCAA ACTAGGTGTT 
CCAGGATTGC CCGGCTATCC AGGACGTCAA GGGCCAAAGG GTTCGACCGG TTTCCCCGGA 
TTCCCAGGAG CCAATGGAGA GAAAGGAGGC AGGGGGGTGG CTGGGAAAGC TGGTCCAAGA 
GGCCAAAGAG GTCCAACGGG CCCCCGTGGT GCACGAGGTG CAAGAGGCCC AACAGGAAAG 
CCAGGCCCGA AGGGAACAGC AGGCAACGAC GGTCCACCTG GCCCACCCGG TGAGAGAGGC 
CCTCAAGGAC CCCAGGGTCC TGTTGGTTTC CCAGGACCAA AGGGTCCCCC TGGACCACCT 
GGAAAGGATG GGCTGCCCGG TCACCCTGGC CAGCGGGGAG AGACTGGATT CCAAGGCAAA 
ACTGGCCCAC CAGGACCTGG AGGTGTTGTT GGACCACAAG GACCGACTGG AGAAACCGGT 
CCAATTGGTG AAAGAGGACA TCCTGGACCC CCTGGTCCCC CCGGAGAGCA GGGTCTTCCT 
GGCGCTGGTG GAAAAGAAGG GGCAAAGGGA GATCCAGGTC CTCAGGGCAG TCCAGGAAAA 
GACGGTCCAC CAGGACTCAG AGGGTTCCCT GGAGAAAGAG GTCTTCCAGG AGTGACGGGT 
CCACCTGGTT TGAAAGGCGC CGAGGGCCCT CAGGGCCCTC CTGGCCCTGT TGGTTCCCCT 
GGTGAGAGAG GAGCAGCAGG ATCTGCTGGT CCCATTGGCC TGCCTGGACG TACTGGACCT 
CAGGGACCCC CAGGACCTGC TGGAGAGAAG GGTGCACCGG GAGAGAGAGG TCCTCTGGGT 
CCAGCCGGTA GGGATGGAGT TCAAGGTCCA GTTGGTCTGC CAGGTCCAGC AGGACCTCAG 
GGTCCACCTG GAGAAGATGG TGACAAGGGA GAAGTGGGTG AACCAGGCCA GAAGGGAAGC 
AAAGCTGACA AAGGAGAACA GGGTCCACCA GGTCCTCCTG GTCTTCAGGG ACCCATTGGA 
GCTCCTGGAC CTGCTGGAGC AGATGGCGAG CCCGGTCCTC GTGGACAGCA GGGTATGTTT 
GGACAGAAGG GAGATGAAGG ATCCCGGGGC TTCCCTGGAC CTCCTGGTCC CATCGGTCTG 
CAGGGTCTTC CAGGACCACC TGGCGAGAAA GGTGAAAATG GAGATGTTGG TCCTATGGGT 
CCTCCTGGTC CACCAGGTCC CAGAGGACCT CAGGGTCCTC CTGGTGCTGA TGGCCCGCAA 
GGTCCTCCTG GTGGAATAGG AGGAATGGGA GCCGTGGGTG AGAAGGGTGA GCAAGGAGAA 
GCTGGCAACC CTGGACCTCC TGGAGAGCCT GGACCTGGGG GCCCGAAAGG TGAGAGAGGA 
GAGAAAGGTG AAGCTGGTCC CCCTGGTGCT GCTGGACCTG CAGGTCCCAA AGGACCCCCT 
GGAGATGATG GACCCAAGGG TAACCCTGGC CCTGTTGGTT TTCCTGGAGA CCCAGGTCCA 
CCTGGTGAGC CAGGTGTTGC TGGAATAGAT GGAGGCCCAG GAGAGAAGGG AGAGGATGGA 
GAGTCTGGTC AGCCTGGACC CCCTGGTCCT TCTGGAGAAG CTGGTCCACC AGGACCTCCT 
GGAAAACGAG GACCCCCGGG GGCGCATGGA GCTGAAGGCA GACAAGGAGA GAAGGGCGCC 
AAGGGAGAAG CAGGACTTGA GGGTCCACCA GGTAAAACTG GACCAGTTGG TCCTCAAGGA 
CCCTCTGGAA AGCCTGGTCC TGAAGGTCTC AGAGGAATCC CCGGTCCAGT CGGTGAACAA 
GGACTTCCTG GTGCTCCTGG ACAAGATGGT CCACCTGGAC CAATGGGACC TCCTGGTCTG 
CCTGGCCTTA AAGGAGACCC TGGCTCTAAG GGTGAGAAGG GTCACCCTGG TCTTATCGGT 
CTCATCGGGC CGCCCGGTGA ACAGGGAGAG AAGGGAGATC GGGGTTTGCC TGGACCTCAG 
GGTGCTGCCG GCAGTAAGGG TGATAATGGC ATCAGTGGTG CTTCTGGTCC AATTGGTCCT 
CCTGGTCCTC CTGGATTGCC TGGTCCACAA GGGCCAAAGG GATCCAAGGG TTCAACTGGT 
CCTGCTGGTC AAAAAGGAGA CACTGGCACG GTTGGACCTC CTGGGCCTCC TGGTCCTCCT 
GGTGAGGTCA TCCAGCCTCT GCCCATCCAC AGTCCCAAGA AATCCCGTCG CTCCATCGAC 
ATGCAGGCGG ATGAAGCAGG CACCATGCTG GACTATGGAG AGGGCATGGA GGACATATTC 
AGCTCTCTCA ATAATCTCAA ACAGGACATC GAGCGCATGA AGTACCCCAT GGGCACGCAA 
AACAACCCAG CACGAAGCTG TAAAGACCTT CAGCTCGCTC ACCCTGAATT CCCAGATGGC 
GAATATTACA TTGATCCTAA CCAGGGCTGC TCTGGAGACT CCTTCAAAGT GTACTGCAAC 
TTCACAGCAG GAGGAGAGAC CTGCATCTTC CCTGATAAAA AGTCAAATGG AGTGAGAATA 
TCTTCATGGC CCAAAGAGGT GCCCGGCTCC TGGTTCAGTG AATTTAAGCG TGGCAAAATA 
CTTTCATACG TGGACGCAGA AGGCAACTCG ATAAACATGG TCCAGATGAC GTTCCTCAAA 
CTACTGACAG CCTCCGCCAG GCAAAACCTG ACCTACAGCT GCCACCAGTC AGTGGCCTGG 
CACGACGCCA CTACGGACAG CTATGACAGA GCATTACACT TCCTCGGCTC CAACGACGAG 
GAGATCTCAT ACGACAACAA CCCCTACATC AAGGCCCTGT CAGACGGCTG TGCGGTGAGG 
AAGGGCTATG GAAAGACAGT ACTGGAGATT AACACTCCCA AAATTGATCA GGTTCCAATC 
GTCGACGTGA AGTTCACTGA CTTTGGGGAT CCCAACCAGA AATTTGGATT TGAAGTTGGT 
CCAGTCTGCT TCCTTGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325884.1
CDS	282..5342
Translation	MERPRWSPMWKTKRWLLDSTVFKLITVSLVLVLQAPHVRAAEPVDVLKVLEFPTVPEGVEKTSGFCTNRRASQPDAAYKISKNAQLSAPTKQLFPDGVFPEDFSILTTIKPKAGIQSFLLSIYNEKGVQQLGVEVGRSPVFLYEDQEGKPAPEDYPLFRALNLADGKWHRVGISVEKKTVTIVVDCKKKLTKPLGRSDHASIDTKGITVFGTRILDEESFEGDIQQLLIVADPKAAYDYCEHYSPDCDVPHKDTLQAQEPGEESNNVDGEYYTDSELDYGTVDASKTPVATTGPNKLGGGAALGQKGEKGEPAVIEPGMLIEGPSGPPGPAGLPGPPGLQGPPGLAGDPGDRGPPGRAGLPGADGLPGPPGTMLMLPFRFGGDGEKGPVVSAQEAQAQAILSQARLAMRGPPGPMGLTGRSGPVGLPGPPGLKGESGDHGPQGPRGIQGPPGQIGKPGKRGRAGADGARGMPGESGSKGDRGFDGLPGLPGEKGHRGETGPAGPPGPTGEDGPRGEDGEIGPRGLAGESGPRGLLGPRGPPGPPGQPGIAGVDGPQGPKGNMGPQGEPGPPGQQGIPGGQGLPGPQGPIGPPGEKGPQGRSGLPGLPGADGPPGHPGKEGPPGEKGALGQPGPQGPIGYPGPRGVKGADGVRGLKGGKGEKGEDGFPGFKGDMGLKGDRGELGLAGPRGEDGPEGPKGRAGPNGESGPQGSAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGGRGVAGKAGPRGQRGPTGPRGARGARGPTGKPGPKGTAGNDGPPGPPGERGPQGPQGPVGFPGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPGGVVGPQGPTGETGPIGERGHPGPPGPPGEQGLPGAGGKEGAKGDPGPQGSPGKDGPPGLRGFPGERGLPGVTGPPGLKGAEGPQGPPGPVGSPGERGAAGSAGPIGLPGRTGPQGPPGPAGEKGAPGERGPLGPAGRDGVQGPVGLPGPAGPQGPPGEDGDKGEVGEPGQKGSKADKGEQGPPGPPGLQGPIGAPGPAGADGEPGPRGQQGMFGQKGDEGSRGFPGPPGPIGLQGLPGPPGEKGENGDVGPMGPPGPPGPRGPQGPPGADGPQGPPGGIGGMGAVGEKGEQGEAGNPGPPGEPGPGGPKGERGEKGEAGPPGAAGPAGPKGPPGDDGPKGNPGPVGFPGDPGPPGEPGVAGIDGGPGEKGEDGESGQPGPPGPSGEAGPPGPPGKRGPPGAHGAEGRQGEKGAKGEAGLEGPPGKTGPVGPQGPSGKPGPEGLRGIPGPVGEQGLPGAPGQDGPPGPMGPPGLPGLKGDPGSKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGAAGSKGDNGISGASGPIGPPGPPGLPGPQGPKGSKGSTGPAGQKGDTGTVGPPGPPGPPGEVIQPLPIHSPKKSRRSIDMQADEAGTMLDYGEGMEDIFSSLNNLKQDIERMKYPMGTQNNPARSCKDLQLAHPEFPDGEYYIDPNQGCSGDSFKVYCNFTAGGETCIFPDKKSNGVRISSWPKEVPGSWFSEFKRGKILSYVDAEGNSINMVQMTFLKLLTASARQNLTYSCHQSVAWHDATTDSYDRALHFLGSNDEEISYDNNPYIKALSDGCAVRKGYGKTVLEINTPKIDQVPIVDVKFTDFGDPNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	XM_021470209.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XI, alpha 1a (col11a1a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470209.1

ORF Insert Sequence:

ATGGAGAGGC CAAGGTGGTC CCCAATGTGG AAAACGAAAA GGTGGCTTTT GGATTCCACT 
GTTTTCAAAT TGATCACAGT TTCCCTCGTC CTGGTTCTGC AGGCTCCACA TGTCAGAGCA 
GCAGAACCCG TGGATGTCTT AAAAGTATTA GAATTTCCCA CTGTCCCAGA AGGAGTAGAG 
AAAACGTCAG GATTCTGCAC AAACCGAAGA GCATCACAGC CAGACGCGGC CTACAAAATC 
AGCAAGAATG CCCAGCTCAG TGCCCCGACC AAGCAGCTTT TCCCAGATGG CGTTTTCCCT 
GAAGACTTCT CCATCCTAAC CACGATCAAG CCCAAAGCAG GGATCCAGTC CTTCCTCCTG 
TCCATCTACA ATGAGAAAGG CGTCCAGCAG CTGGGCGTGG AGGTGGGCCG TTCACCTGTC 
TTCCTGTACG AGGATCAGGA GGGCAAACCT GCCCCGGAGG ATTACCCTCT GTTCCGCGCT 
CTCAATCTGG CCGACGGAAA GTGGCATCGC GTAGGCATCA GCGTTGAGAA GAAGACAGTG 
ACTATTGTTG TCGACTGTAA GAAGAAGCTG ACCAAGCCAT TGGGCAGGAG TGACCATGCC 
AGTATCGACA CCAAGGGTAT CACCGTTTTT GGCACCAGGA TCCTGGATGA GGAATCTTTT 
GAGGGTGATA TACAACAACT GCTGATTGTG GCTGACCCTA AAGCAGCTTA CGACTACTGT 
GAACATTACA GCCCAGACTG TGACGTCCCT CACAAGGACA CACTGCAGGC ACAGGAGCCC 
GGAGAGGAGT CTAACAATGT AGATGGAGAG TACTATACAG ACAGTGAACT TGACTACGGA 
ACAGTAGATG CTTCCAAAAC GCCTGTCGCA ACCACTGGAC CAAACAAGCT GGGTGGAGGT 
GCTGCTCTTG GTCAGAAAGG TGAGAAAGGA GAGCCTGCAG TGATTGAACC AGGAATGCTG 
ATCGAGGGAC CTTCAGGACC ACCTGGACCT GCTGGTCTTC CAGGTCCTCC AGGTTTACAA 
GGCCCCCCTG GTTTGGCTGG AGATCCTGGT GATAGGGGTC CTCCAGGCCG TGCTGGTCTT 
CCTGGAGCTG ATGGTTTGCC AGGACCACCT GGAACTATGC TCATGCTGCC GTTTCGTTTT 
GGTGGTGATG GAGAAAAGGG CCCAGTGGTT TCAGCTCAGG AGGCTCAGGC TCAGGCTATT 
CTCTCTCAGG CCAGGCTCGC CATGAGAGGT CCACCGGGTC CAATGGGTCT CACTGGACGA 
TCTGGACCAG TGGGTCTTCC AGGTCCTCCT GGACTGAAGG GAGAAAGTGG GGATCATGGT 
CCTCAGGGTC CAAGGGGAAT CCAAGGCCCA CCAGGGCAAA TAGGAAAACC TGGAAAGAGG 
GGTCGTGCTG GAGCTGATGG TGCCCGAGGA ATGCCTGGAG AGTCTGGATC TAAGGGTGAC 
AGAGGTTTCG ATGGGCTTCC GGGTCTGCCT GGTGAGAAAG GACACAGGGG TGAGACCGGC 
CCTGCTGGAC CCCCGGGACC AACTGGAGAG GATGGACCAA GAGGAGAAGA TGGTGAGATC 
GGACCAAGAG GACTTGCTGG TGAGAGCGGG CCGCGAGGTC TTCTCGGCCC AAGAGGCCCT 
CCTGGACCTC CTGGACAACC TGGCATTGCT GGTGTTGATG GTCCGCAGGG TCCCAAAGGA 
AACATGGGGC CTCAGGGAGA GCCAGGTCCT CCTGGACAGC AGGGAATCCC AGGAGGACAG 
GGTCTTCCAG GTCCACAGGG ACCCATCGGA CCACCCGGAG AAAAAGGCCC TCAGGGAAGG 
TCTGGTTTGC CTGGATTGCC TGGTGCAGAT GGGCCTCCTG GACATCCTGG TAAAGAGGGT 
CCACCTGGAG AGAAAGGAGC TTTGGGCCAG CCTGGTCCCC AGGGCCCCAT CGGCTATCCT 
GGCCCTCGCG GTGTGAAGGG TGCAGATGGT GTCCGTGGTC TAAAGGGAGG CAAAGGAGAG 
AAGGGTGAGG ATGGATTCCC AGGTTTCAAG GGAGACATGG GTCTCAAAGG CGACAGGGGA 
GAGCTGGGAC TGGCTGGACC CAGAGGAGAA GATGGTCCAG AGGGCCCGAA GGGTCGTGCA 
GGGCCTAACG GTGAATCTGG ACCCCAGGGA TCTGCCGGAG AAAAGGGCAA ACTAGGTGTT 
CCAGGATTGC CCGGCTATCC AGGACGTCAA GGGCCAAAGG GTTCGACCGG TTTCCCCGGA 
TTCCCAGGAG CCAATGGAGA GAAAGGAGGC AGGGGGGTGG CTGGGAAAGC TGGTCCAAGA 
GGCCAAAGAG GTCCAACGGG CCCCCGTGGT GCACGAGGTG CAAGAGGCCC AACAGGAAAG 
CCAGGCCCGA AGGGAACAGC AGGCAACGAC GGTCCACCTG GCCCACCCGG TGAGAGAGGC 
CCTCAAGGAC CCCAGGGTCC TGTTGGTTTC CCAGGACCAA AGGGTCCCCC TGGACCACCT 
GGAAAGGATG GGCTGCCCGG TCACCCTGGC CAGCGGGGAG AGACTGGATT CCAAGGCAAA 
ACTGGCCCAC CAGGACCTGG AGGTGTTGTT GGACCACAAG GACCGACTGG AGAAACCGGT 
CCAATTGGTG AAAGAGGACA TCCTGGACCC CCTGGTCCCC CCGGAGAGCA GGGTCTTCCT 
GGCGCTGGTG GAAAAGAAGG GGCAAAGGGA GATCCAGGTC CTCAGGGCAG TCCAGGAAAA 
GACGGTCCAC CAGGACTCAG AGGGTTCCCT GGAGAAAGAG GTCTTCCAGG AGTGACGGGT 
CCACCTGGTT TGAAAGGCGC CGAGGGCCCT CAGGGCCCTC CTGGCCCTGT TGGTTCCCCT 
GGTGAGAGAG GAGCAGCAGG ATCTGCTGGT CCCATTGGCC TGCCTGGACG TACTGGACCT 
CAGGGACCCC CAGGACCTGC TGGAGAGAAG GGTGCACCGG GAGAGAGAGG TCCTCTGGGT 
CCAGCCGGTA GGGATGGAGT TCAAGGTCCA GTTGGTCTGC CAGGTCCAGC AGGACCTCAG 
GGTCCACCTG GAGAAGATGG TGACAAGGGA GAAGTGGGTG AACCAGGCCA GAAGGGAAGC 
AAAGCTGACA AAGGAGAACA GGGTCCACCA GGTCCTCCTG GTCTTCAGGG ACCCATTGGA 
GCTCCTGGAC CTGCTGGAGC AGATGGCGAG CCCGGTCCTC GTGGACAGCA GGGTATGTTT 
GGACAGAAGG GAGATGAAGG ATCCCGGGGC TTCCCTGGAC CTCCTGGTCC CATCGGTCTG 
CAGGGTCTTC CAGGACCACC TGGCGAGAAA GGTGAAAATG GAGATGTTGG TCCTATGGGT 
CCTCCTGGTC CACCAGGTCC CAGAGGACCT CAGGGTCCTC CTGGTGCTGA TGGCCCGCAA 
GGTCCTCCTG GTGGAATAGG AGGAATGGGA GCCGTGGGTG AGAAGGGTGA GCAAGGAGAA 
GCTGGCAACC CTGGACCTCC TGGAGAGCCT GGACCTGGGG GCCCGAAAGG TGAGAGAGGA 
GAGAAAGGTG AAGCTGGTCC CCCTGGTGCT GCTGGACCTG CAGGTCCCAA AGGACCCCCT 
GGAGATGATG GACCCAAGGG TAACCCTGGC CCTGTTGGTT TTCCTGGAGA CCCAGGTCCA 
CCTGGTGAGC CAGGTGTTGC TGGAATAGAT GGAGGCCCAG GAGAGAAGGG AGAGGATGGA 
GAGTCTGGTC AGCCTGGACC CCCTGGTCCT TCTGGAGAAG CTGGTCCACC AGGACCTCCT 
GGAAAACGAG GACCCCCGGG GGCGCATGGA GCTGAAGGCA GACAAGGAGA GAAGGGCGCC 
AAGGGAGAAG CAGGACTTGA GGGTCCACCA GGTAAAACTG GACCAGTTGG TCCTCAAGGA 
CCCTCTGGAA AGCCTGGTCC TGAAGGTCTC AGAGGAATCC CCGGTCCAGT CGGTGAACAA 
GGACTTCCTG GTGCTCCTGG ACAAGATGGT CCACCTGGAC CAATGGGACC TCCTGGTCTG 
CCTGGCCTTA AAGGAGACCC TGGCTCTAAG GGTGAGAAGG GTCACCCTGG TCTTATCGGT 
CTCATCGGGC CGCCCGGTGA ACAGGGAGAG AAGGGAGATC GGGGTTTGCC TGGACCTCAG 
GGTGCTGCCG GCAGTAAGGG TGATAATGGC ATCAGTGGTG CTTCTGGTCC AATTGGTCCT 
CCTGGTCCTC CTGGATTGCC TGGTCCACAA GGGCCAAAGG GATCCAAGGG TTCAACTGGT 
CCTGCTGGTC AAAAAGGAGA CACTGGCACG GTTGGACCTC CTGGGCCTCC TGGTCCTCCT 
GGTGAGGTCA TCCAGCCTCT GCCCATCCAC AGTCCCAAGA AATCCCGTCG CTCCATCGAC 
ATGCAGGCGG ATGAAGCAGG CACCATGCTG GACTATGGAG AGGGCATGGA GGACATATTC 
AGCTCTCTCA ATAATCTCAA ACAGGACATC GAGCGCATGA AGTACCCCAT GGGCACGCAA 
AACAACCCAG CACGAAGCTG TAAAGACCTT CAGCTCGCTC ACCCTGAATT CCCAGATGGC 
GAATATTACA TTGATCCTAA CCAGGGCTGC TCTGGAGACT CCTTCAAAGT GTACTGCAAC 
TTCACAGCAG GAGGAGAGAC CTGCATCTTC CCTGATAAAA AGTCAAATGG AGTGAGAATA 
TCTTCATGGC CCAAAGAGGT GCCCGGCTCC TGGTTCAGTG AATTTAAGCG TGGCAAAATA 
CTTTCATACG TGGACGCAGA AGGCAACTCG ATAAACATGG TCCAGATGAC GTTCCTCAAA 
CTACTGACAG CCTCCGCCAG GCAAAACCTG ACCTACAGCT GCCACCAGTC AGTGGCCTGG 
CACGACGCCA CTACGGACAG CTATGACAGA GCATTACACT TCCTCGGCTC CAACGACGAG 
GAGATCTCAT ACGACAACAA CCCCTACATC AAGGCCCTGT CAGACGGCTG TGCGGTGAGG 
AAGGGCTATG GAAAGACAGT ACTGGAGATT AACACTCCCA AAATTGATCA GGTTCCAATC 
GTCGACGTGA AGTTCACTGA CTTTGGGGAT CCCAACCAGA AATTTGGATT TGAAGTTGGT 
CCAGTCTGCT TCCTTGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col11a1a ( XM_021470210.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325885.1 Danio rerio collagen, type XI, alpha 1a (col11a1a), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa151721
Clone ID Related Accession (Same CDS sequence)	XM_021470210.1
Accession Version	XM_021470210.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-1(XI) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCA AGAGGACAAA AAAGTCCCCT AAATCTTTTG CCAAACCCTA TAAGTACCTG 
GAGCCAAAAC AGGCTAAAAA GTTAGTGCCT AAGAAGAAGA GATACGGATA CCTGCCACCA 
ACACCAAGAC AAGCCATTCT AGGGCTGGGT GGAGGTGCTG CTCTTGGTCA GAAAGGTGAG 
AAAGGAGAGC CTGCAGTGAT TGAACCAGGA ATGCTGATCG AGGGACCTTC AGGACCACCT 
GGACCTGCTG GTCTTCCAGG TCCTCCAGGT TTACAAGGCC CCCCTGGTTT GGCTGGAGAT 
CCTGGTGATA GGGGTCCTCC AGGCCGTGCT GGTCTTCCTG GAGCTGATGG TTTGCCAGGA 
CCACCTGGAA CTATGCTCAT GCTGCCGTTT CGTTTTGGTG GTGATGGAGA AAAGGGCCCA 
GTGGTTTCAG CTCAGGAGGC TCAGGCTCAG GCTATTCTCT CTCAGGCCAG GCTCGCCATG 
AGAGGTCCAC CGGGTCCAAT GGGTCTCACT GGACGATCTG GACCAGTGGG TCTTCCAGGT 
CCTCCTGGAC TGAAGGGAGA AAGTGGGGAT CATGGTCCTC AGGGTCCAAG GGGAATCCAA 
GGCCCACCAG GGCAAATAGG AAAACCTGGA AAGAGGGGTC GTGCTGGAGC TGATGGTGCC 
CGAGGAATGC CTGGAGAGTC TGGATCTAAG GGTGACAGAG GTTTCGATGG GCTTCCGGGT 
CTGCCTGGTG AGAAAGGACA CAGGGGTGAG ACCGGCCCTG CTGGACCCCC GGGACCAACT 
GGAGAGGATG GACCAAGAGG AGAAGATGGT GAGATCGGAC CAAGAGGACT TGCTGGTGAG 
AGCGGGCCGC GAGGTCTTCT CGGCCCAAGA GGCCCTCCTG GACCTCCTGG ACAACCTGGC 
ATTGCTGGTG TTGATGGTCC GCAGGGTCCC AAAGGAAACA TGGGGCCTCA GGGAGAGCCA 
GGTCCTCCTG GACAGCAGGG AATCCCAGGA GGACAGGGTC TTCCAGGTCC ACAGGGACCC 
ATCGGACCAC CCGGAGAAAA AGGCCCTCAG GGAAGGTCTG GTTTGCCTGG ATTGCCTGGT 
GCAGATGGGC CTCCTGGACA TCCTGGTAAA GAGGGTCCAC CTGGAGAGAA AGGAGCTTTG 
GGCCAGCCTG GTCCCCAGGG CCCCATCGGC TATCCTGGCC CTCGCGGTGT GAAGGGTGCA 
GATGGTGTCC GTGGTCTAAA GGGAGGCAAA GGAGAGAAGG GTGAGGATGG ATTCCCAGGT 
TTCAAGGGAG ACATGGGTCT CAAAGGCGAC AGGGGAGAGC TGGGACTGGC TGGACCCAGA 
GGAGAAGATG GTCCAGAGGG CCCGAAGGGT CGTGCAGGGC CTAACGGTGA ATCTGGACCC 
CAGGGATCTG CCGGAGAAAA GGGCAAACTA GGTGTTCCAG GATTGCCCGG CTATCCAGGA 
CGTCAAGGGC CAAAGGGTTC GACCGGTTTC CCCGGATTCC CAGGAGCCAA TGGAGAGAAA 
GGAGGCAGGG GGGTGGCTGG GAAAGCTGGT CCAAGAGGCC AAAGAGGTCC AACGGGCCCC 
CGTGGTGCAC GAGGTGCAAG AGGCCCAACA GGAAAGCCAG GCCCGAAGGG AACAGCAGGC 
AACGACGGTC CACCTGGCCC ACCCGGTGAG AGAGGCCCTC AAGGACCCCA GGGTCCTGTT 
GGTTTCCCAG GACCAAAGGG TCCCCCTGGA CCACCTGGAA AGGATGGGCT GCCCGGTCAC 
CCTGGCCAGC GGGGAGAGAC TGGATTCCAA GGCAAAACTG GCCCACCAGG ACCTGGAGGT 
GTTGTTGGAC CACAAGGACC GACTGGAGAA ACCGGTCCAA TTGGTGAAAG AGGACATCCT 
GGACCCCCTG GTCCCCCCGG AGAGCAGGGT CTTCCTGGCG CTGGTGGAAA AGAAGGGGCA 
AAGGGAGATC CAGGTCCTCA GGGCAGTCCA GGAAAAGACG GTCCACCAGG ACTCAGAGGG 
TTCCCTGGAG AAAGAGGTCT TCCAGGAGTG ACGGGTCCAC CTGGTTTGAA AGGCGCCGAG 
GGCCCTCAGG GCCCTCCTGG CCCTGTTGGT TCCCCTGGTG AGAGAGGAGC AGCAGGATCT 
GCTGGTCCCA TTGGCCTGCC TGGACGTACT GGACCTCAGG GACCCCCAGG ACCTGCTGGA 
GAGAAGGGTG CACCGGGAGA GAGAGGTCCT CTGGGTCCAG CCGGTAGGGA TGGAGTTCAA 
GGTCCAGTTG GTCTGCCAGG TCCAGCAGGA CCTCAGGGTC CACCTGGAGA AGATGGTGAC 
AAGGGAGAAG TGGGTGAACC AGGCCAGAAG GGAAGCAAAG CTGACAAAGG AGAACAGGGT 
CCACCAGGTC CTCCTGGTCT TCAGGGACCC ATTGGAGCTC CTGGACCTGC TGGAGCAGAT 
GGCGAGCCCG GTCCTCGTGG ACAGCAGGGT ATGTTTGGAC AGAAGGGAGA TGAAGGATCC 
CGGGGCTTCC CTGGACCTCC TGGTCCCATC GGTCTGCAGG GTCTTCCAGG ACCACCTGGC 
GAGAAAGGTG AAAATGGAGA TGTTGGTCCT ATGGGTCCTC CTGGTCCACC AGGTCCCAGA 
GGACCTCAGG GTCCTCCTGG TGCTGATGGC CCGCAAGGTC CTCCTGGTGG AATAGGAGGA 
ATGGGAGCCG TGGGTGAGAA GGGTGAGCAA GGAGAAGCTG GCAACCCTGG ACCTCCTGGA 
GAGCCTGGAC CTGGGGGCCC GAAAGGTGAG AGAGGAGAGA AAGGTGAAGC TGGTCCCCCT 
GGTGCTGCTG GACCTGCAGG TCCCAAAGGA CCCCCTGGAG ATGATGGACC CAAGGGTAAC 
CCTGGCCCTG TTGGTTTTCC TGGAGACCCA GGTCCACCTG GTGAGCCAGG TGTTGCTGGA 
ATAGATGGAG GCCCAGGAGA GAAGGGAGAG GATGGAGAGT CTGGTCAGCC TGGACCCCCT 
GGTCCTTCTG GAGAAGCTGG TCCACCAGGA CCTCCTGGAA AACGAGGACC CCCGGGGGCG 
CATGGAGCTG AAGGCAGACA AGGAGAGAAG GGCGCCAAGG GAGAAGCAGG ACTTGAGGGT 
CCACCAGGTA AAACTGGACC AGTTGGTCCT CAAGGACCCT CTGGAAAGCC TGGTCCTGAA 
GGTCTCAGAG GAATCCCCGG TCCAGTCGGT GAACAAGGAC TTCCTGGTGC TCCTGGACAA 
GATGGTCCAC CTGGACCAAT GGGACCTCCT GGTCTGCCTG GCCTTAAAGG AGACCCTGGC 
TCTAAGGGTG AGAAGGGTCA CCCTGGTCTT ATCGGTCTCA TCGGGCCGCC CGGTGAACAG 
GGAGAGAAGG GAGATCGGGG TTTGCCTGGA CCTCAGGGTG CTGCCGGCAG TAAGGGTGAT 
AATGGCATCA GTGGTGCTTC TGGTCCAATT GGTCCTCCTG GTCCTCCTGG ATTGCCTGGT 
CCACAAGGGC CAAAGGGATC CAAGGGTTCA ACTGGTCCTG CTGGTCAAAA AGGAGACACT 
GGCACGGTTG GACCTCCTGG GCCTCCTGGT CCTCCTGGTG AGGTCATCCA GCCTCTGCCC 
ATCCACAGTC CCAAGAAATC CCGTCGCTCC ATCGACATGC AGGCGGATGA AGCAGGCACC 
ATGCTGGACT ATGGAGAGGG CATGGAGGAC ATATTCAGCT CTCTCAATAA TCTCAAACAG 
GACATCGAGC GCATGAAGTA CCCCATGGGC ACGCAAAACA ACCCAGCACG AAGCTGTAAA 
GACCTTCAGC TCGCTCACCC TGAATTCCCA GATGGCGAAT ATTACATTGA TCCTAACCAG 
GGCTGCTCTG GAGACTCCTT CAAAGTGTAC TGCAACTTCA CAGCAGGAGG AGAGACCTGC 
ATCTTCCCTG ATAAAAAGTC AAATGGAGTG AGAATATCTT CATGGCCCAA AGAGGTGCCC 
GGCTCCTGGT TCAGTGAATT TAAGCGTGGC AAAATACTTT CATACGTGGA CGCAGAAGGC 
AACTCGATAA ACATGGTCCA GATGACGTTC CTCAAACTAC TGACAGCCTC CGCCAGGCAA 
AACCTGACCT ACAGCTGCCA CCAGTCAGTG GCCTGGCACG ACGCCACTAC GGACAGCTAT 
GACAGAGCAT TACACTTCCT CGGCTCCAAC GACGAGGAGA TCTCATACGA CAACAACCCC 
TACATCAAGG CCCTGTCAGA CGGCTGTGCG GTGAGGAAGG GCTATGGAAA GACAGTACTG 
GAGATTAACA CTCCCAAAAT TGATCAGGTT CCAATCGTCG ACGTGAAGTT CACTGACTTT 
GGGGATCCCA ACCAGAAATT TGGATTTGAA GTTGGTCCAG TCTGCTTCCT TGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325885.1
CDS	190..4506
Translation	MKSKRTKKSPKSFAKPYKYLEPKQAKKLVPKKKRYGYLPPTPRQAILGLGGGAALGQKGEKGEPAVIEPGMLIEGPSGPPGPAGLPGPPGLQGPPGLAGDPGDRGPPGRAGLPGADGLPGPPGTMLMLPFRFGGDGEKGPVVSAQEAQAQAILSQARLAMRGPPGPMGLTGRSGPVGLPGPPGLKGESGDHGPQGPRGIQGPPGQIGKPGKRGRAGADGARGMPGESGSKGDRGFDGLPGLPGEKGHRGETGPAGPPGPTGEDGPRGEDGEIGPRGLAGESGPRGLLGPRGPPGPPGQPGIAGVDGPQGPKGNMGPQGEPGPPGQQGIPGGQGLPGPQGPIGPPGEKGPQGRSGLPGLPGADGPPGHPGKEGPPGEKGALGQPGPQGPIGYPGPRGVKGADGVRGLKGGKGEKGEDGFPGFKGDMGLKGDRGELGLAGPRGEDGPEGPKGRAGPNGESGPQGSAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGGRGVAGKAGPRGQRGPTGPRGARGARGPTGKPGPKGTAGNDGPPGPPGERGPQGPQGPVGFPGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPGGVVGPQGPTGETGPIGERGHPGPPGPPGEQGLPGAGGKEGAKGDPGPQGSPGKDGPPGLRGFPGERGLPGVTGPPGLKGAEGPQGPPGPVGSPGERGAAGSAGPIGLPGRTGPQGPPGPAGEKGAPGERGPLGPAGRDGVQGPVGLPGPAGPQGPPGEDGDKGEVGEPGQKGSKADKGEQGPPGPPGLQGPIGAPGPAGADGEPGPRGQQGMFGQKGDEGSRGFPGPPGPIGLQGLPGPPGEKGENGDVGPMGPPGPPGPRGPQGPPGADGPQGPPGGIGGMGAVGEKGEQGEAGNPGPPGEPGPGGPKGERGEKGEAGPPGAAGPAGPKGPPGDDGPKGNPGPVGFPGDPGPPGEPGVAGIDGGPGEKGEDGESGQPGPPGPSGEAGPPGPPGKRGPPGAHGAEGRQGEKGAKGEAGLEGPPGKTGPVGPQGPSGKPGPEGLRGIPGPVGEQGLPGAPGQDGPPGPMGPPGLPGLKGDPGSKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGAAGSKGDNGISGASGPIGPPGPPGLPGPQGPKGSKGSTGPAGQKGDTGTVGPPGPPGPPGEVIQPLPIHSPKKSRRSIDMQADEAGTMLDYGEGMEDIFSSLNNLKQDIERMKYPMGTQNNPARSCKDLQLAHPEFPDGEYYIDPNQGCSGDSFKVYCNFTAGGETCIFPDKKSNGVRISSWPKEVPGSWFSEFKRGKILSYVDAEGNSINMVQMTFLKLLTASARQNLTYSCHQSVAWHDATTDSYDRALHFLGSNDEEISYDNNPYIKALSDGCAVRKGYGKTVLEINTPKIDQVPIVDVKFTDFGDPNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	XM_021470210.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XI, alpha 1a (col11a1a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470210.1

ORF Insert Sequence:

ATGAAATCCA AGAGGACAAA AAAGTCCCCT AAATCTTTTG CCAAACCCTA TAAGTACCTG 
GAGCCAAAAC AGGCTAAAAA GTTAGTGCCT AAGAAGAAGA GATACGGATA CCTGCCACCA 
ACACCAAGAC AAGCCATTCT AGGGCTGGGT GGAGGTGCTG CTCTTGGTCA GAAAGGTGAG 
AAAGGAGAGC CTGCAGTGAT TGAACCAGGA ATGCTGATCG AGGGACCTTC AGGACCACCT 
GGACCTGCTG GTCTTCCAGG TCCTCCAGGT TTACAAGGCC CCCCTGGTTT GGCTGGAGAT 
CCTGGTGATA GGGGTCCTCC AGGCCGTGCT GGTCTTCCTG GAGCTGATGG TTTGCCAGGA 
CCACCTGGAA CTATGCTCAT GCTGCCGTTT CGTTTTGGTG GTGATGGAGA AAAGGGCCCA 
GTGGTTTCAG CTCAGGAGGC TCAGGCTCAG GCTATTCTCT CTCAGGCCAG GCTCGCCATG 
AGAGGTCCAC CGGGTCCAAT GGGTCTCACT GGACGATCTG GACCAGTGGG TCTTCCAGGT 
CCTCCTGGAC TGAAGGGAGA AAGTGGGGAT CATGGTCCTC AGGGTCCAAG GGGAATCCAA 
GGCCCACCAG GGCAAATAGG AAAACCTGGA AAGAGGGGTC GTGCTGGAGC TGATGGTGCC 
CGAGGAATGC CTGGAGAGTC TGGATCTAAG GGTGACAGAG GTTTCGATGG GCTTCCGGGT 
CTGCCTGGTG AGAAAGGACA CAGGGGTGAG ACCGGCCCTG CTGGACCCCC GGGACCAACT 
GGAGAGGATG GACCAAGAGG AGAAGATGGT GAGATCGGAC CAAGAGGACT TGCTGGTGAG 
AGCGGGCCGC GAGGTCTTCT CGGCCCAAGA GGCCCTCCTG GACCTCCTGG ACAACCTGGC 
ATTGCTGGTG TTGATGGTCC GCAGGGTCCC AAAGGAAACA TGGGGCCTCA GGGAGAGCCA 
GGTCCTCCTG GACAGCAGGG AATCCCAGGA GGACAGGGTC TTCCAGGTCC ACAGGGACCC 
ATCGGACCAC CCGGAGAAAA AGGCCCTCAG GGAAGGTCTG GTTTGCCTGG ATTGCCTGGT 
GCAGATGGGC CTCCTGGACA TCCTGGTAAA GAGGGTCCAC CTGGAGAGAA AGGAGCTTTG 
GGCCAGCCTG GTCCCCAGGG CCCCATCGGC TATCCTGGCC CTCGCGGTGT GAAGGGTGCA 
GATGGTGTCC GTGGTCTAAA GGGAGGCAAA GGAGAGAAGG GTGAGGATGG ATTCCCAGGT 
TTCAAGGGAG ACATGGGTCT CAAAGGCGAC AGGGGAGAGC TGGGACTGGC TGGACCCAGA 
GGAGAAGATG GTCCAGAGGG CCCGAAGGGT CGTGCAGGGC CTAACGGTGA ATCTGGACCC 
CAGGGATCTG CCGGAGAAAA GGGCAAACTA GGTGTTCCAG GATTGCCCGG CTATCCAGGA 
CGTCAAGGGC CAAAGGGTTC GACCGGTTTC CCCGGATTCC CAGGAGCCAA TGGAGAGAAA 
GGAGGCAGGG GGGTGGCTGG GAAAGCTGGT CCAAGAGGCC AAAGAGGTCC AACGGGCCCC 
CGTGGTGCAC GAGGTGCAAG AGGCCCAACA GGAAAGCCAG GCCCGAAGGG AACAGCAGGC 
AACGACGGTC CACCTGGCCC ACCCGGTGAG AGAGGCCCTC AAGGACCCCA GGGTCCTGTT 
GGTTTCCCAG GACCAAAGGG TCCCCCTGGA CCACCTGGAA AGGATGGGCT GCCCGGTCAC 
CCTGGCCAGC GGGGAGAGAC TGGATTCCAA GGCAAAACTG GCCCACCAGG ACCTGGAGGT 
GTTGTTGGAC CACAAGGACC GACTGGAGAA ACCGGTCCAA TTGGTGAAAG AGGACATCCT 
GGACCCCCTG GTCCCCCCGG AGAGCAGGGT CTTCCTGGCG CTGGTGGAAA AGAAGGGGCA 
AAGGGAGATC CAGGTCCTCA GGGCAGTCCA GGAAAAGACG GTCCACCAGG ACTCAGAGGG 
TTCCCTGGAG AAAGAGGTCT TCCAGGAGTG ACGGGTCCAC CTGGTTTGAA AGGCGCCGAG 
GGCCCTCAGG GCCCTCCTGG CCCTGTTGGT TCCCCTGGTG AGAGAGGAGC AGCAGGATCT 
GCTGGTCCCA TTGGCCTGCC TGGACGTACT GGACCTCAGG GACCCCCAGG ACCTGCTGGA 
GAGAAGGGTG CACCGGGAGA GAGAGGTCCT CTGGGTCCAG CCGGTAGGGA TGGAGTTCAA 
GGTCCAGTTG GTCTGCCAGG TCCAGCAGGA CCTCAGGGTC CACCTGGAGA AGATGGTGAC 
AAGGGAGAAG TGGGTGAACC AGGCCAGAAG GGAAGCAAAG CTGACAAAGG AGAACAGGGT 
CCACCAGGTC CTCCTGGTCT TCAGGGACCC ATTGGAGCTC CTGGACCTGC TGGAGCAGAT 
GGCGAGCCCG GTCCTCGTGG ACAGCAGGGT ATGTTTGGAC AGAAGGGAGA TGAAGGATCC 
CGGGGCTTCC CTGGACCTCC TGGTCCCATC GGTCTGCAGG GTCTTCCAGG ACCACCTGGC 
GAGAAAGGTG AAAATGGAGA TGTTGGTCCT ATGGGTCCTC CTGGTCCACC AGGTCCCAGA 
GGACCTCAGG GTCCTCCTGG TGCTGATGGC CCGCAAGGTC CTCCTGGTGG AATAGGAGGA 
ATGGGAGCCG TGGGTGAGAA GGGTGAGCAA GGAGAAGCTG GCAACCCTGG ACCTCCTGGA 
GAGCCTGGAC CTGGGGGCCC GAAAGGTGAG AGAGGAGAGA AAGGTGAAGC TGGTCCCCCT 
GGTGCTGCTG GACCTGCAGG TCCCAAAGGA CCCCCTGGAG ATGATGGACC CAAGGGTAAC 
CCTGGCCCTG TTGGTTTTCC TGGAGACCCA GGTCCACCTG GTGAGCCAGG TGTTGCTGGA 
ATAGATGGAG GCCCAGGAGA GAAGGGAGAG GATGGAGAGT CTGGTCAGCC TGGACCCCCT 
GGTCCTTCTG GAGAAGCTGG TCCACCAGGA CCTCCTGGAA AACGAGGACC CCCGGGGGCG 
CATGGAGCTG AAGGCAGACA AGGAGAGAAG GGCGCCAAGG GAGAAGCAGG ACTTGAGGGT 
CCACCAGGTA AAACTGGACC AGTTGGTCCT CAAGGACCCT CTGGAAAGCC TGGTCCTGAA 
GGTCTCAGAG GAATCCCCGG TCCAGTCGGT GAACAAGGAC TTCCTGGTGC TCCTGGACAA 
GATGGTCCAC CTGGACCAAT GGGACCTCCT GGTCTGCCTG GCCTTAAAGG AGACCCTGGC 
TCTAAGGGTG AGAAGGGTCA CCCTGGTCTT ATCGGTCTCA TCGGGCCGCC CGGTGAACAG 
GGAGAGAAGG GAGATCGGGG TTTGCCTGGA CCTCAGGGTG CTGCCGGCAG TAAGGGTGAT 
AATGGCATCA GTGGTGCTTC TGGTCCAATT GGTCCTCCTG GTCCTCCTGG ATTGCCTGGT 
CCACAAGGGC CAAAGGGATC CAAGGGTTCA ACTGGTCCTG CTGGTCAAAA AGGAGACACT 
GGCACGGTTG GACCTCCTGG GCCTCCTGGT CCTCCTGGTG AGGTCATCCA GCCTCTGCCC 
ATCCACAGTC CCAAGAAATC CCGTCGCTCC ATCGACATGC AGGCGGATGA AGCAGGCACC 
ATGCTGGACT ATGGAGAGGG CATGGAGGAC ATATTCAGCT CTCTCAATAA TCTCAAACAG 
GACATCGAGC GCATGAAGTA CCCCATGGGC ACGCAAAACA ACCCAGCACG AAGCTGTAAA 
GACCTTCAGC TCGCTCACCC TGAATTCCCA GATGGCGAAT ATTACATTGA TCCTAACCAG 
GGCTGCTCTG GAGACTCCTT CAAAGTGTAC TGCAACTTCA CAGCAGGAGG AGAGACCTGC 
ATCTTCCCTG ATAAAAAGTC AAATGGAGTG AGAATATCTT CATGGCCCAA AGAGGTGCCC 
GGCTCCTGGT TCAGTGAATT TAAGCGTGGC AAAATACTTT CATACGTGGA CGCAGAAGGC 
AACTCGATAA ACATGGTCCA GATGACGTTC CTCAAACTAC TGACAGCCTC CGCCAGGCAA 
AACCTGACCT ACAGCTGCCA CCAGTCAGTG GCCTGGCACG ACGCCACTAC GGACAGCTAT 
GACAGAGCAT TACACTTCCT CGGCTCCAAC GACGAGGAGA TCTCATACGA CAACAACCCC 
TACATCAAGG CCCTGTCAGA CGGCTGTGCG GTGAGGAAGG GCTATGGAAA GACAGTACTG 
GAGATTAACA CTCCCAAAAT TGATCAGGTT CCAATCGTCG ACGTGAAGTT CACTGACTTT 
GGGGATCCCA ACCAGAAATT TGGATTTGAA GTTGGTCCAG TCTGCTTCCT TGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col11a1a ( XM_005162813.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162870.1 Danio rerio collagen, type XI, alpha 1a (col11a1a), transcript variant X1, mRNA.

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CloneID	ODa28707
Clone ID Related Accession (Same CDS sequence)	XM_005162813.1
Accession Version	XM_005162813.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-1(XI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGGC CAAGGTGGTC CCCAATGTGG AAAACGAAAA GGTGGCTTTT GGATTCCACT 
GTTTTCAAAT TGATCACAGT TTCCCTCGTC CTGGTTCTGC AGGCTCCACA TGTCAGAGCA 
GCAGAACCCG TGGATGTCTT AAAAGTATTA GAATTTCCCA CTGTCCCAGA AGGAGTAGAG 
AAAACGTCAG GATTCTGCAC AAACCGAAGA GCATCACAGC CAGACGCGGC CTACAAAATC 
AGCAAGAATG CCCAGCTCAG TGCCCCGACC AAGCAGCTTT TCCCAGATGG CGTTTTCCCT 
GAAGACTTCT CCATCCTAAC CACGATCAAG CCCAAAGCAG GGATCCAGTC CTTCCTCCTG 
TCCATCTACA ATGAGAAAGG CGTCCAGCAG CTGGGCGTGG AGGTGGGCCG TTCACCTGTC 
TTCCTGTACG AGGATCAGGA GGGCAAACCT GCCCCGGAGG ATTACCCTCT GTTCCGCGCT 
CTCAATCTGG CCGACGGAAA GTGGCATCGC GTAGGCATCA GCGTTGAGAA GAAGACAGTG 
ACTATTGTTG TCGACTGTAA GAAGAAGCTG ACCAAGCCAT TGGGCAGGAG TGACCATGCC 
AGTATCGACA CCAAGGGTAT CACCGTTTTT GGCACCAGGA TCCTGGATGA GGAATCTTTT 
GAGGGTGATA TACAACAACT GCTGATTGTG GCTGACCCTA AAGCAGCTTA CGACTACTGT 
GAACATTACA GCCCAGACTG TGACGTCCCT CACAAGGACA CACTGCAGGC ACAGGAGCCC 
GGAGAGGAGT ACACAACTCC TGTTGAATAT GGTGATTTAT ATGATTATTA CGAGGGAGGC 
ACACCCACAG CGACTGATGA GTTTTCTGAG CCGCAGTCTA ACAATGTAGA TGGAGAGTAC 
TATACAGACA GTGAACTTGA CTACGGAACA GTAGATGCTT CCAAAACGCC TGTCGCAACC 
ACTGGACCAA ACAAGGCCAT GGAAGAGGAG AATTTTGGTG ATTATATCAC CGAGACGGCC 
CAGGCTGGTC TGGATCCGAC CGCTGCCTCA GAAGCTGTGC TGCCCAGTGG CACCGCTGAC 
CCCATCGGAG TGGACTCGTA TGACTTTAAG GAGTATGATC TTAAGGAATA TGATGTGACT 
GGGCTTGACA AAAATCAGTA TGAGTATGGG CCTTATGATG AGTTTGGCAA TGTGGCTCCA 
AACCCAACAT CCAATTACGA GGACACTTTT GGGCCTGGGG TTGCGGCCGA AACCGACATC 
GCTGAAACAG ACCTGGGTGG AGGTGCTGCT CTTGGTCAGA AAGGTGAGAA AGGAGAGCCT 
GCAGTGATTG AACCAGGAAT GCTGATCGAG GGACCTTCAG GACCACCTGG ACCTGCTGGT 
CTTCCAGGTC CTCCAGGTTT ACAAGGCCCC CCTGGTTTGG CTGGAGATCC TGGTGATAGG 
GGTCCTCCAG GCCGTGCTGG TCTTCCTGGA GCTGATGGTT TGCCAGGACC ACCTGGAACT 
ATGCTCATGC TGCCGTTTCG TTTTGGTGGT GATGGAGAAA AGGGCCCAGT GGTTTCAGCT 
CAGGAGGCTC AGGCTCAGGC TATTCTCTCT CAGGCCAGGC TCGCCATGAG AGGTCCACCG 
GGTCCAATGG GTCTCACTGG ACGATCTGGA CCAGTGGGTC TTCCAGGTCC TCCTGGACTG 
AAGGGAGAAA GTGGGGATCA TGGTCCTCAG GGTCCAAGGG GAATCCAAGG CCCACCAGGG 
CAAATAGGAA AACCTGGAAA GAGGGGTCGT GCTGGAGCTG ATGGTGCCCG AGGAATGCCT 
GGAGAGTCTG GATCTAAGGG TGACAGAGGT TTCGATGGGC TTCCGGGTCT GCCTGGTGAG 
AAAGGACACA GGGGTGAGAC CGGCCCTGCT GGACCCCCGG GACCAACTGG AGAGGATGGA 
CCAAGAGGAG AAGATGGTGA GATCGGACCA AGAGGACTTG CTGGTGAGAG CGGGCCGCGA 
GGTCTTCTCG GCCCAAGAGG CCCTCCTGGA CCTCCTGGAC AACCTGGCAT TGCTGGTGTT 
GATGGTCCGC AGGGTCCCAA AGGAAACATG GGGCCTCAGG GAGAGCCAGG TCCTCCTGGA 
CAGCAGGGAA TCCCAGGAGG ACAGGGTCTT CCAGGTCCAC AGGGACCCAT CGGACCACCC 
GGAGAAAAAG GCCCTCAGGG AAGGTCTGGT TTGCCTGGAT TGCCTGGTGC AGATGGGCCT 
CCTGGACATC CTGGTAAAGA GGGTCCACCT GGAGAGAAAG GAGCTTTGGG CCAGCCTGGT 
CCCCAGGGCC CCATCGGCTA TCCTGGCCCT CGCGGTGTGA AGGGTGCAGA TGGTGTCCGT 
GGTCTAAAGG GAGGCAAAGG AGAGAAGGGT GAGGATGGAT TCCCAGGTTT CAAGGGAGAC 
ATGGGTCTCA AAGGCGACAG GGGAGAGCTG GGACTGGCTG GACCCAGAGG AGAAGATGGT 
CCAGAGGGCC CGAAGGGTCG TGCAGGGCCT AACGGTGAAT CTGGACCCCA GGGATCTGCC 
GGAGAAAAGG GCAAACTAGG TGTTCCAGGA TTGCCCGGCT ATCCAGGACG TCAAGGGCCA 
AAGGGTTCGA CCGGTTTCCC CGGATTCCCA GGAGCCAATG GAGAGAAAGG AGGCAGGGGG 
GTGGCTGGGA AAGCTGGTCC AAGAGGCCAA AGAGGTCCAA CGGGCCCCCG TGGTGCACGA 
GGTGCAAGAG GCCCAACAGG AAAGCCAGGC CCGAAGGGAA CAGCAGGCAA CGACGGTCCA 
CCTGGCCCAC CCGGTGAGAG AGGCCCTCAA GGACCCCAGG GTCCTGTTGG TTTCCCAGGA 
CCAAAGGGTC CCCCTGGACC ACCTGGAAAG GATGGGCTGC CCGGTCACCC TGGCCAGCGG 
GGAGAGACTG GATTCCAAGG CAAAACTGGC CCACCAGGAC CTGGAGGTGT TGTTGGACCA 
CAAGGACCGA CTGGAGAAAC CGGTCCAATT GGTGAAAGAG GACATCCTGG ACCCCCTGGT 
CCCCCCGGAG AGCAGGGTCT TCCTGGCGCT GGTGGAAAAG AAGGGGCAAA GGGAGATCCA 
GGTCCTCAGG GCAGTCCAGG AAAAGACGGT CCACCAGGAC TCAGAGGGTT CCCTGGAGAA 
AGAGGTCTTC CAGGAGTGAC GGGTCCACCT GGTTTGAAAG GCGCCGAGGG CCCTCAGGGC 
CCTCCTGGCC CTGTTGGTTC CCCTGGTGAG AGAGGAGCAG CAGGATCTGC TGGTCCCATT 
GGCCTGCCTG GACGTACTGG ACCTCAGGGA CCCCCAGGAC CTGCTGGAGA GAAGGGTGCA 
CCGGGAGAGA GAGGTCCTCT GGGTCCAGCC GGTAGGGATG GAGTTCAAGG TCCAGTTGGT 
CTGCCAGGTC CAGCAGGACC TCAGGGTCCA CCTGGAGAAG ATGGTGACAA GGGAGAAGTG 
GGTGAACCAG GCCAGAAGGG AAGCAAAGCT GACAAAGGAG AACAGGGTCC ACCAGGTCCT 
CCTGGTCTTC AGGGACCCAT TGGAGCTCCT GGACCTGCTG GAGCAGATGG CGAGCCCGGT 
CCTCGTGGAC AGCAGGGTAT GTTTGGACAG AAGGGAGATG AAGGATCCCG GGGCTTCCCT 
GGACCTCCTG GTCCCATCGG TCTGCAGGGT CTTCCAGGAC CACCTGGCGA GAAAGGTGAA 
AATGGAGATG TTGGTCCTAT GGGTCCTCCT GGTCCACCAG GTCCCAGAGG ACCTCAGGGT 
CCTCCTGGTG CTGATGGCCC GCAAGGTCCT CCTGGTGGAA TAGGAGGAAT GGGAGCCGTG 
GGTGAGAAGG GTGAGCAAGG AGAAGCTGGC AACCCTGGAC CTCCTGGAGA GCCTGGACCT 
GGGGGCCCGA AAGGTGAGAG AGGAGAGAAA GGTGAAGCTG GTCCCCCTGG TGCTGCTGGA 
CCTGCAGGTC CCAAAGGACC CCCTGGAGAT GATGGACCCA AGGGTAACCC TGGCCCTGTT 
GGTTTTCCTG GAGACCCAGG TCCACCTGGT GAGCCAGGTG TTGCTGGAAT AGATGGAGGC 
CCAGGAGAGA AGGGAGAGGA TGGAGAGTCT GGTCAGCCTG GACCCCCTGG TCCTTCTGGA 
GAAGCTGGTC CACCAGGACC TCCTGGAAAA CGAGGACCCC CGGGGGCGCA TGGAGCTGAA 
GGCAGACAAG GAGAGAAGGG CGCCAAGGGA GAAGCAGGAC TTGAGGGTCC ACCAGGTAAA 
ACTGGACCAG TTGGTCCTCA AGGACCCTCT GGAAAGCCTG GTCCTGAAGG TCTCAGAGGA 
ATCCCCGGTC CAGTCGGTGA ACAAGGACTT CCTGGTGCTC CTGGACAAGA TGGTCCACCT 
GGACCAATGG GACCTCCTGG TCTGCCTGGC CTTAAAGGAG ACCCTGGCTC TAAGGGTGAG 
AAGGGTCACC CTGGTCTTAT CGGTCTCATC GGGCCGCCCG GTGAACAGGG AGAGAAGGGA 
GATCGGGGTT TGCCTGGACC TCAGGGTGCT GCCGGCAGTA AGGGTGATAA TGGCATCAGT 
GGTGCTTCTG GTCCAATTGG TCCTCCTGGT CCTCCTGGAT TGCCTGGTCC ACAAGGGCCA 
AAGGGATCCA AGGGTTCAAC TGGTCCTGCT GGTCAAAAAG GAGACACTGG CACGGTTGGA 
CCTCCTGGGC CTCCTGGTCC TCCTGGTGAG GTCATCCAGC CTCTGCCCAT CCACAGTCCC 
AAGAAATCCC GTCGCTCCAT CGACATGCAG GCGGATGAAG CAGGCACCAT GCTGGACTAT 
GGAGAGGGCA TGGAGGACAT ATTCAGCTCT CTCAATAATC TCAAACAGGA CATCGAGCGC 
ATGAAGTACC CCATGGGCAC GCAAAACAAC CCAGCACGAA GCTGTAAAGA CCTTCAGCTC 
GCTCACCCTG AATTCCCAGA TGGCGAATAT TACATTGATC CTAACCAGGG CTGCTCTGGA 
GACTCCTTCA AAGTGTACTG CAACTTCACA GCAGGAGGAG AGACCTGCAT CTTCCCTGAT 
AAAAAGTCAA ATGGAGTGAG AATATCTTCA TGGCCCAAAG AGGTGCCCGG CTCCTGGTTC 
AGTGAATTTA AGCGTGGCAA AATACTTTCA TACGTGGACG CAGAAGGCAA CTCGATAAAC 
ATGGTCCAGA TGACGTTCCT CAAACTACTG ACAGCCTCCG CCAGGCAAAA CCTGACCTAC 
AGCTGCCACC AGTCAGTGGC CTGGCACGAC GCCACTACGG ACAGCTATGA CAGAGCATTA 
CACTTCCTCG GCTCCAACGA CGAGGAGATC TCATACGACA ACAACCCCTA CATCAAGGCC 
CTGTCAGACG GCTGTGCGGT GAGGAAGGGC TATGGAAAGA CAGTACTGGA GATTAACACT 
CCCAAAATTG ATCAGGTTCC AATCGTCGAC GTGAAGTTCA CTGACTTTGG GGATCCCAAC 
CAGAAATTTG GATTTGAAGT TGGTCCAGTC TGCTTCCTTG GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162870.1
CDS	282..5726
Translation	MERPRWSPMWKTKRWLLDSTVFKLITVSLVLVLQAPHVRAAEPVDVLKVLEFPTVPEGVEKTSGFCTNRRASQPDAAYKISKNAQLSAPTKQLFPDGVFPEDFSILTTIKPKAGIQSFLLSIYNEKGVQQLGVEVGRSPVFLYEDQEGKPAPEDYPLFRALNLADGKWHRVGISVEKKTVTIVVDCKKKLTKPLGRSDHASIDTKGITVFGTRILDEESFEGDIQQLLIVADPKAAYDYCEHYSPDCDVPHKDTLQAQEPGEEYTTPVEYGDLYDYYEGGTPTATDEFSEPQSNNVDGEYYTDSELDYGTVDASKTPVATTGPNKAMEEENFGDYITETAQAGLDPTAASEAVLPSGTADPIGVDSYDFKEYDLKEYDVTGLDKNQYEYGPYDEFGNVAPNPTSNYEDTFGPGVAAETDIAETDLGGGAALGQKGEKGEPAVIEPGMLIEGPSGPPGPAGLPGPPGLQGPPGLAGDPGDRGPPGRAGLPGADGLPGPPGTMLMLPFRFGGDGEKGPVVSAQEAQAQAILSQARLAMRGPPGPMGLTGRSGPVGLPGPPGLKGESGDHGPQGPRGIQGPPGQIGKPGKRGRAGADGARGMPGESGSKGDRGFDGLPGLPGEKGHRGETGPAGPPGPTGEDGPRGEDGEIGPRGLAGESGPRGLLGPRGPPGPPGQPGIAGVDGPQGPKGNMGPQGEPGPPGQQGIPGGQGLPGPQGPIGPPGEKGPQGRSGLPGLPGADGPPGHPGKEGPPGEKGALGQPGPQGPIGYPGPRGVKGADGVRGLKGGKGEKGEDGFPGFKGDMGLKGDRGELGLAGPRGEDGPEGPKGRAGPNGESGPQGSAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGGRGVAGKAGPRGQRGPTGPRGARGARGPTGKPGPKGTAGNDGPPGPPGERGPQGPQGPVGFPGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPGGVVGPQGPTGETGPIGERGHPGPPGPPGEQGLPGAGGKEGAKGDPGPQGSPGKDGPPGLRGFPGERGLPGVTGPPGLKGAEGPQGPPGPVGSPGERGAAGSAGPIGLPGRTGPQGPPGPAGEKGAPGERGPLGPAGRDGVQGPVGLPGPAGPQGPPGEDGDKGEVGEPGQKGSKADKGEQGPPGPPGLQGPIGAPGPAGADGEPGPRGQQGMFGQKGDEGSRGFPGPPGPIGLQGLPGPPGEKGENGDVGPMGPPGPPGPRGPQGPPGADGPQGPPGGIGGMGAVGEKGEQGEAGNPGPPGEPGPGGPKGERGEKGEAGPPGAAGPAGPKGPPGDDGPKGNPGPVGFPGDPGPPGEPGVAGIDGGPGEKGEDGESGQPGPPGPSGEAGPPGPPGKRGPPGAHGAEGRQGEKGAKGEAGLEGPPGKTGPVGPQGPSGKPGPEGLRGIPGPVGEQGLPGAPGQDGPPGPMGPPGLPGLKGDPGSKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGAAGSKGDNGISGASGPIGPPGPPGLPGPQGPKGSKGSTGPAGQKGDTGTVGPPGPPGPPGEVIQPLPIHSPKKSRRSIDMQADEAGTMLDYGEGMEDIFSSLNNLKQDIERMKYPMGTQNNPARSCKDLQLAHPEFPDGEYYIDPNQGCSGDSFKVYCNFTAGGETCIFPDKKSNGVRISSWPKEVPGSWFSEFKRGKILSYVDAEGNSINMVQMTFLKLLTASARQNLTYSCHQSVAWHDATTDSYDRALHFLGSNDEEISYDNNPYIKALSDGCAVRKGYGKTVLEINTPKIDQVPIVDVKFTDFGDPNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	XM_005162813.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XI, alpha 1a (col11a1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162813.1

ORF Insert Sequence:

ATGGAGAGGC CAAGGTGGTC CCCAATGTGG AAAACGAAAA GGTGGCTTTT GGATTCCACT 
GTTTTCAAAT TGATCACAGT TTCCCTCGTC CTGGTTCTGC AGGCTCCACA TGTCAGAGCA 
GCAGAACCCG TGGATGTCTT AAAAGTATTA GAATTTCCCA CTGTCCCAGA AGGAGTAGAG 
AAAACGTCAG GATTCTGCAC AAACCGAAGA GCATCACAGC CAGACGCGGC CTACAAAATC 
AGCAAGAATG CCCAGCTCAG TGCCCCGACC AAGCAGCTTT TCCCAGATGG CGTTTTCCCT 
GAAGACTTCT CCATCCTAAC CACGATCAAG CCCAAAGCAG GGATCCAGTC CTTCCTCCTG 
TCCATCTACA ATGAGAAAGG CGTCCAGCAG CTGGGCGTGG AGGTGGGCCG TTCACCTGTC 
TTCCTGTACG AGGATCAGGA GGGCAAACCT GCCCCGGAGG ATTACCCTCT GTTCCGCGCT 
CTCAATCTGG CCGACGGAAA GTGGCATCGC GTAGGCATCA GCGTTGAGAA GAAGACAGTG 
ACTATTGTTG TCGACTGTAA GAAGAAGCTG ACCAAGCCAT TGGGCAGGAG TGACCATGCC 
AGTATCGACA CCAAGGGTAT CACCGTTTTT GGCACCAGGA TCCTGGATGA GGAATCTTTT 
GAGGGTGATA TACAACAACT GCTGATTGTG GCTGACCCTA AAGCAGCTTA CGACTACTGT 
GAACATTACA GCCCAGACTG TGACGTCCCT CACAAGGACA CACTGCAGGC ACAGGAGCCC 
GGAGAGGAGT ACACAACTCC TGTTGAATAT GGTGATTTAT ATGATTATTA CGAGGGAGGC 
ACACCCACAG CGACTGATGA GTTTTCTGAG CCGCAGTCTA ACAATGTAGA TGGAGAGTAC 
TATACAGACA GTGAACTTGA CTACGGAACA GTAGATGCTT CCAAAACGCC TGTCGCAACC 
ACTGGACCAA ACAAGGCCAT GGAAGAGGAG AATTTTGGTG ATTATATCAC CGAGACGGCC 
CAGGCTGGTC TGGATCCGAC CGCTGCCTCA GAAGCTGTGC TGCCCAGTGG CACCGCTGAC 
CCCATCGGAG TGGACTCGTA TGACTTTAAG GAGTATGATC TTAAGGAATA TGATGTGACT 
GGGCTTGACA AAAATCAGTA TGAGTATGGG CCTTATGATG AGTTTGGCAA TGTGGCTCCA 
AACCCAACAT CCAATTACGA GGACACTTTT GGGCCTGGGG TTGCGGCCGA AACCGACATC 
GCTGAAACAG ACCTGGGTGG AGGTGCTGCT CTTGGTCAGA AAGGTGAGAA AGGAGAGCCT 
GCAGTGATTG AACCAGGAAT GCTGATCGAG GGACCTTCAG GACCACCTGG ACCTGCTGGT 
CTTCCAGGTC CTCCAGGTTT ACAAGGCCCC CCTGGTTTGG CTGGAGATCC TGGTGATAGG 
GGTCCTCCAG GCCGTGCTGG TCTTCCTGGA GCTGATGGTT TGCCAGGACC ACCTGGAACT 
ATGCTCATGC TGCCGTTTCG TTTTGGTGGT GATGGAGAAA AGGGCCCAGT GGTTTCAGCT 
CAGGAGGCTC AGGCTCAGGC TATTCTCTCT CAGGCCAGGC TCGCCATGAG AGGTCCACCG 
GGTCCAATGG GTCTCACTGG ACGATCTGGA CCAGTGGGTC TTCCAGGTCC TCCTGGACTG 
AAGGGAGAAA GTGGGGATCA TGGTCCTCAG GGTCCAAGGG GAATCCAAGG CCCACCAGGG 
CAAATAGGAA AACCTGGAAA GAGGGGTCGT GCTGGAGCTG ATGGTGCCCG AGGAATGCCT 
GGAGAGTCTG GATCTAAGGG TGACAGAGGT TTCGATGGGC TTCCGGGTCT GCCTGGTGAG 
AAAGGACACA GGGGTGAGAC CGGCCCTGCT GGACCCCCGG GACCAACTGG AGAGGATGGA 
CCAAGAGGAG AAGATGGTGA GATCGGACCA AGAGGACTTG CTGGTGAGAG CGGGCCGCGA 
GGTCTTCTCG GCCCAAGAGG CCCTCCTGGA CCTCCTGGAC AACCTGGCAT TGCTGGTGTT 
GATGGTCCGC AGGGTCCCAA AGGAAACATG GGGCCTCAGG GAGAGCCAGG TCCTCCTGGA 
CAGCAGGGAA TCCCAGGAGG ACAGGGTCTT CCAGGTCCAC AGGGACCCAT CGGACCACCC 
GGAGAAAAAG GCCCTCAGGG AAGGTCTGGT TTGCCTGGAT TGCCTGGTGC AGATGGGCCT 
CCTGGACATC CTGGTAAAGA GGGTCCACCT GGAGAGAAAG GAGCTTTGGG CCAGCCTGGT 
CCCCAGGGCC CCATCGGCTA TCCTGGCCCT CGCGGTGTGA AGGGTGCAGA TGGTGTCCGT 
GGTCTAAAGG GAGGCAAAGG AGAGAAGGGT GAGGATGGAT TCCCAGGTTT CAAGGGAGAC 
ATGGGTCTCA AAGGCGACAG GGGAGAGCTG GGACTGGCTG GACCCAGAGG AGAAGATGGT 
CCAGAGGGCC CGAAGGGTCG TGCAGGGCCT AACGGTGAAT CTGGACCCCA GGGATCTGCC 
GGAGAAAAGG GCAAACTAGG TGTTCCAGGA TTGCCCGGCT ATCCAGGACG TCAAGGGCCA 
AAGGGTTCGA CCGGTTTCCC CGGATTCCCA GGAGCCAATG GAGAGAAAGG AGGCAGGGGG 
GTGGCTGGGA AAGCTGGTCC AAGAGGCCAA AGAGGTCCAA CGGGCCCCCG TGGTGCACGA 
GGTGCAAGAG GCCCAACAGG AAAGCCAGGC CCGAAGGGAA CAGCAGGCAA CGACGGTCCA 
CCTGGCCCAC CCGGTGAGAG AGGCCCTCAA GGACCCCAGG GTCCTGTTGG TTTCCCAGGA 
CCAAAGGGTC CCCCTGGACC ACCTGGAAAG GATGGGCTGC CCGGTCACCC TGGCCAGCGG 
GGAGAGACTG GATTCCAAGG CAAAACTGGC CCACCAGGAC CTGGAGGTGT TGTTGGACCA 
CAAGGACCGA CTGGAGAAAC CGGTCCAATT GGTGAAAGAG GACATCCTGG ACCCCCTGGT 
CCCCCCGGAG AGCAGGGTCT TCCTGGCGCT GGTGGAAAAG AAGGGGCAAA GGGAGATCCA 
GGTCCTCAGG GCAGTCCAGG AAAAGACGGT CCACCAGGAC TCAGAGGGTT CCCTGGAGAA 
AGAGGTCTTC CAGGAGTGAC GGGTCCACCT GGTTTGAAAG GCGCCGAGGG CCCTCAGGGC 
CCTCCTGGCC CTGTTGGTTC CCCTGGTGAG AGAGGAGCAG CAGGATCTGC TGGTCCCATT 
GGCCTGCCTG GACGTACTGG ACCTCAGGGA CCCCCAGGAC CTGCTGGAGA GAAGGGTGCA 
CCGGGAGAGA GAGGTCCTCT GGGTCCAGCC GGTAGGGATG GAGTTCAAGG TCCAGTTGGT 
CTGCCAGGTC CAGCAGGACC TCAGGGTCCA CCTGGAGAAG ATGGTGACAA GGGAGAAGTG 
GGTGAACCAG GCCAGAAGGG AAGCAAAGCT GACAAAGGAG AACAGGGTCC ACCAGGTCCT 
CCTGGTCTTC AGGGACCCAT TGGAGCTCCT GGACCTGCTG GAGCAGATGG CGAGCCCGGT 
CCTCGTGGAC AGCAGGGTAT GTTTGGACAG AAGGGAGATG AAGGATCCCG GGGCTTCCCT 
GGACCTCCTG GTCCCATCGG TCTGCAGGGT CTTCCAGGAC CACCTGGCGA GAAAGGTGAA 
AATGGAGATG TTGGTCCTAT GGGTCCTCCT GGTCCACCAG GTCCCAGAGG ACCTCAGGGT 
CCTCCTGGTG CTGATGGCCC GCAAGGTCCT CCTGGTGGAA TAGGAGGAAT GGGAGCCGTG 
GGTGAGAAGG GTGAGCAAGG AGAAGCTGGC AACCCTGGAC CTCCTGGAGA GCCTGGACCT 
GGGGGCCCGA AAGGTGAGAG AGGAGAGAAA GGTGAAGCTG GTCCCCCTGG TGCTGCTGGA 
CCTGCAGGTC CCAAAGGACC CCCTGGAGAT GATGGACCCA AGGGTAACCC TGGCCCTGTT 
GGTTTTCCTG GAGACCCAGG TCCACCTGGT GAGCCAGGTG TTGCTGGAAT AGATGGAGGC 
CCAGGAGAGA AGGGAGAGGA TGGAGAGTCT GGTCAGCCTG GACCCCCTGG TCCTTCTGGA 
GAAGCTGGTC CACCAGGACC TCCTGGAAAA CGAGGACCCC CGGGGGCGCA TGGAGCTGAA 
GGCAGACAAG GAGAGAAGGG CGCCAAGGGA GAAGCAGGAC TTGAGGGTCC ACCAGGTAAA 
ACTGGACCAG TTGGTCCTCA AGGACCCTCT GGAAAGCCTG GTCCTGAAGG TCTCAGAGGA 
ATCCCCGGTC CAGTCGGTGA ACAAGGACTT CCTGGTGCTC CTGGACAAGA TGGTCCACCT 
GGACCAATGG GACCTCCTGG TCTGCCTGGC CTTAAAGGAG ACCCTGGCTC TAAGGGTGAG 
AAGGGTCACC CTGGTCTTAT CGGTCTCATC GGGCCGCCCG GTGAACAGGG AGAGAAGGGA 
GATCGGGGTT TGCCTGGACC TCAGGGTGCT GCCGGCAGTA AGGGTGATAA TGGCATCAGT 
GGTGCTTCTG GTCCAATTGG TCCTCCTGGT CCTCCTGGAT TGCCTGGTCC ACAAGGGCCA 
AAGGGATCCA AGGGTTCAAC TGGTCCTGCT GGTCAAAAAG GAGACACTGG CACGGTTGGA 
CCTCCTGGGC CTCCTGGTCC TCCTGGTGAG GTCATCCAGC CTCTGCCCAT CCACAGTCCC 
AAGAAATCCC GTCGCTCCAT CGACATGCAG GCGGATGAAG CAGGCACCAT GCTGGACTAT 
GGAGAGGGCA TGGAGGACAT ATTCAGCTCT CTCAATAATC TCAAACAGGA CATCGAGCGC 
ATGAAGTACC CCATGGGCAC GCAAAACAAC CCAGCACGAA GCTGTAAAGA CCTTCAGCTC 
GCTCACCCTG AATTCCCAGA TGGCGAATAT TACATTGATC CTAACCAGGG CTGCTCTGGA 
GACTCCTTCA AAGTGTACTG CAACTTCACA GCAGGAGGAG AGACCTGCAT CTTCCCTGAT 
AAAAAGTCAA ATGGAGTGAG AATATCTTCA TGGCCCAAAG AGGTGCCCGG CTCCTGGTTC 
AGTGAATTTA AGCGTGGCAA AATACTTTCA TACGTGGACG CAGAAGGCAA CTCGATAAAC 
ATGGTCCAGA TGACGTTCCT CAAACTACTG ACAGCCTCCG CCAGGCAAAA CCTGACCTAC 
AGCTGCCACC AGTCAGTGGC CTGGCACGAC GCCACTACGG ACAGCTATGA CAGAGCATTA 
CACTTCCTCG GCTCCAACGA CGAGGAGATC TCATACGACA ACAACCCCTA CATCAAGGCC 
CTGTCAGACG GCTGTGCGGT GAGGAAGGGC TATGGAAAGA CAGTACTGGA GATTAACACT 
CCCAAAATTG ATCAGGTTCC AATCGTCGAC GTGAAGTTCA CTGACTTTGG GGATCCCAAC 
CAGAAATTTG GATTTGAAGT TGGTCCAGTC TGCTTCCTTG GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col11a1a ( XM_005162814.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162871.1 Danio rerio collagen, type XI, alpha 1a (col11a1a), transcript variant X2, mRNA.

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CloneID	ODa28708
Clone ID Related Accession (Same CDS sequence)	XM_005162814.1
Accession Version	XM_005162814.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-1(XI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGGC CAAGGTGGTC CCCAATGTGG AAAACGAAAA GGTGGCTTTT GGATTCCACT 
GTTTTCAAAT TGATCACAGT TTCCCTCGTC CTGGTTCTGC AGGCTCCACA TGTCAGAGCA 
GAACCCGTGG ATGTCTTAAA AGTATTAGAA TTTCCCACTG TCCCAGAAGG AGTAGAGAAA 
ACGTCAGGAT TCTGCACAAA CCGAAGAGCA TCACAGCCAG ACGCGGCCTA CAAAATCAGC 
AAGAATGCCC AGCTCAGTGC CCCGACCAAG CAGCTTTTCC CAGATGGCGT TTTCCCTGAA 
GACTTCTCCA TCCTAACCAC GATCAAGCCC AAAGCAGGGA TCCAGTCCTT CCTCCTGTCC 
ATCTACAATG AGAAAGGCGT CCAGCAGCTG GGCGTGGAGG TGGGCCGTTC ACCTGTCTTC 
CTGTACGAGG ATCAGGAGGG CAAACCTGCC CCGGAGGATT ACCCTCTGTT CCGCGCTCTC 
AATCTGGCCG ACGGAAAGTG GCATCGCGTA GGCATCAGCG TTGAGAAGAA GACAGTGACT 
ATTGTTGTCG ACTGTAAGAA GAAGCTGACC AAGCCATTGG GCAGGAGTGA CCATGCCAGT 
ATCGACACCA AGGGTATCAC CGTTTTTGGC ACCAGGATCC TGGATGAGGA ATCTTTTGAG 
GGTGATATAC AACAACTGCT GATTGTGGCT GACCCTAAAG CAGCTTACGA CTACTGTGAA 
CATTACAGCC CAGACTGTGA CGTCCCTCAC AAGGACACAC TGCAGGCACA GGAGCCCGGA 
GAGGAGTACA CAACTCCTGT TGAATATGGT GATTTATATG ATTATTACGA GGGAGGCACA 
CCCACAGCGA CTGATGAGTT TTCTGAGCCG CAGTCTAACA ATGTAGATGG AGAGTACTAT 
ACAGACAGTG AACTTGACTA CGGAACAGTA GATGCTTCCA AAACGCCTGT CGCAACCACT 
GGACCAAACA AGGCCATGGA AGAGGAGAAT TTTGGTGATT ATATCACCGA GACGGCCCAG 
GCTGGTCTGG ATCCGACCGC TGCCTCAGAA GCTGTGCTGC CCAGTGGCAC CGCTGACCCC 
ATCGGAGTGG ACTCGTATGA CTTTAAGGAG TATGATCTTA AGGAATATGA TGTGACTGGG 
CTTGACAAAA ATCAGTATGA GTATGGGCCT TATGATGAGT TTGGCAATGT GGCTCCAAAC 
CCAACATCCA ATTACGAGGA CACTTTTGGG CCTGGGGTTG CGGCCGAAAC CGACATCGCT 
GAAACAGACC TGGGTGGAGG TGCTGCTCTT GGTCAGAAAG GTGAGAAAGG AGAGCCTGCA 
GTGATTGAAC CAGGAATGCT GATCGAGGGA CCTTCAGGAC CACCTGGACC TGCTGGTCTT 
CCAGGTCCTC CAGGTTTACA AGGCCCCCCT GGTTTGGCTG GAGATCCTGG TGATAGGGGT 
CCTCCAGGCC GTGCTGGTCT TCCTGGAGCT GATGGTTTGC CAGGACCACC TGGAACTATG 
CTCATGCTGC CGTTTCGTTT TGGTGGTGAT GGAGAAAAGG GCCCAGTGGT TTCAGCTCAG 
GAGGCTCAGG CTCAGGCTAT TCTCTCTCAG GCCAGGCTCG CCATGAGAGG TCCACCGGGT 
CCAATGGGTC TCACTGGACG ATCTGGACCA GTGGGTCTTC CAGGTCCTCC TGGACTGAAG 
GGAGAAAGTG GGGATCATGG TCCTCAGGGT CCAAGGGGAA TCCAAGGCCC ACCAGGGCAA 
ATAGGAAAAC CTGGAAAGAG GGGTCGTGCT GGAGCTGATG GTGCCCGAGG AATGCCTGGA 
GAGTCTGGAT CTAAGGGTGA CAGAGGTTTC GATGGGCTTC CGGGTCTGCC TGGTGAGAAA 
GGACACAGGG GTGAGACCGG CCCTGCTGGA CCCCCGGGAC CAACTGGAGA GGATGGACCA 
AGAGGAGAAG ATGGTGAGAT CGGACCAAGA GGACTTGCTG GTGAGAGCGG GCCGCGAGGT 
CTTCTCGGCC CAAGAGGCCC TCCTGGACCT CCTGGACAAC CTGGCATTGC TGGTGTTGAT 
GGTCCGCAGG GTCCCAAAGG AAACATGGGG CCTCAGGGAG AGCCAGGTCC TCCTGGACAG 
CAGGGAATCC CAGGAGGACA GGGTCTTCCA GGTCCACAGG GACCCATCGG ACCACCCGGA 
GAAAAAGGCC CTCAGGGAAG GTCTGGTTTG CCTGGATTGC CTGGTGCAGA TGGGCCTCCT 
GGACATCCTG GTAAAGAGGG TCCACCTGGA GAGAAAGGAG CTTTGGGCCA GCCTGGTCCC 
CAGGGCCCCA TCGGCTATCC TGGCCCTCGC GGTGTGAAGG GTGCAGATGG TGTCCGTGGT 
CTAAAGGGAG GCAAAGGAGA GAAGGGTGAG GATGGATTCC CAGGTTTCAA GGGAGACATG 
GGTCTCAAAG GCGACAGGGG AGAGCTGGGA CTGGCTGGAC CCAGAGGAGA AGATGGTCCA 
GAGGGCCCGA AGGGTCGTGC AGGGCCTAAC GGTGAATCTG GACCCCAGGG ATCTGCCGGA 
GAAAAGGGCA AACTAGGTGT TCCAGGATTG CCCGGCTATC CAGGACGTCA AGGGCCAAAG 
GGTTCGACCG GTTTCCCCGG ATTCCCAGGA GCCAATGGAG AGAAAGGAGG CAGGGGGGTG 
GCTGGGAAAG CTGGTCCAAG AGGCCAAAGA GGTCCAACGG GCCCCCGTGG TGCACGAGGT 
GCAAGAGGCC CAACAGGAAA GCCAGGCCCG AAGGGAACAG CAGGCAACGA CGGTCCACCT 
GGCCCACCCG GTGAGAGAGG CCCTCAAGGA CCCCAGGGTC CTGTTGGTTT CCCAGGACCA 
AAGGGTCCCC CTGGACCACC TGGAAAGGAT GGGCTGCCCG GTCACCCTGG CCAGCGGGGA 
GAGACTGGAT TCCAAGGCAA AACTGGCCCA CCAGGACCTG GAGGTGTTGT TGGACCACAA 
GGACCGACTG GAGAAACCGG TCCAATTGGT GAAAGAGGAC ATCCTGGACC CCCTGGTCCC 
CCCGGAGAGC AGGGTCTTCC TGGCGCTGGT GGAAAAGAAG GGGCAAAGGG AGATCCAGGT 
CCTCAGGGCA GTCCAGGAAA AGACGGTCCA CCAGGACTCA GAGGGTTCCC TGGAGAAAGA 
GGTCTTCCAG GAGTGACGGG TCCACCTGGT TTGAAAGGCG CCGAGGGCCC TCAGGGCCCT 
CCTGGCCCTG TTGGTTCCCC TGGTGAGAGA GGAGCAGCAG GATCTGCTGG TCCCATTGGC 
CTGCCTGGAC GTACTGGACC TCAGGGACCC CCAGGACCTG CTGGAGAGAA GGGTGCACCG 
GGAGAGAGAG GTCCTCTGGG TCCAGCCGGT AGGGATGGAG TTCAAGGTCC AGTTGGTCTG 
CCAGGTCCAG CAGGACCTCA GGGTCCACCT GGAGAAGATG GTGACAAGGG AGAAGTGGGT 
GAACCAGGCC AGAAGGGAAG CAAAGCTGAC AAAGGAGAAC AGGGTCCACC AGGTCCTCCT 
GGTCTTCAGG GACCCATTGG AGCTCCTGGA CCTGCTGGAG CAGATGGCGA GCCCGGTCCT 
CGTGGACAGC AGGGTATGTT TGGACAGAAG GGAGATGAAG GATCCCGGGG CTTCCCTGGA 
CCTCCTGGTC CCATCGGTCT GCAGGGTCTT CCAGGACCAC CTGGCGAGAA AGGTGAAAAT 
GGAGATGTTG GTCCTATGGG TCCTCCTGGT CCACCAGGTC CCAGAGGACC TCAGGGTCCT 
CCTGGTGCTG ATGGCCCGCA AGGTCCTCCT GGTGGAATAG GAGGAATGGG AGCCGTGGGT 
GAGAAGGGTG AGCAAGGAGA AGCTGGCAAC CCTGGACCTC CTGGAGAGCC TGGACCTGGG 
GGCCCGAAAG GTGAGAGAGG AGAGAAAGGT GAAGCTGGTC CCCCTGGTGC TGCTGGACCT 
GCAGGTCCCA AAGGACCCCC TGGAGATGAT GGACCCAAGG GTAACCCTGG CCCTGTTGGT 
TTTCCTGGAG ACCCAGGTCC ACCTGGTGAG CCAGGTGTTG CTGGAATAGA TGGAGGCCCA 
GGAGAGAAGG GAGAGGATGG AGAGTCTGGT CAGCCTGGAC CCCCTGGTCC TTCTGGAGAA 
GCTGGTCCAC CAGGACCTCC TGGAAAACGA GGACCCCCGG GGGCGCATGG AGCTGAAGGC 
AGACAAGGAG AGAAGGGCGC CAAGGGAGAA GCAGGACTTG AGGGTCCACC AGGTAAAACT 
GGACCAGTTG GTCCTCAAGG ACCCTCTGGA AAGCCTGGTC CTGAAGGTCT CAGAGGAATC 
CCCGGTCCAG TCGGTGAACA AGGACTTCCT GGTGCTCCTG GACAAGATGG TCCACCTGGA 
CCAATGGGAC CTCCTGGTCT GCCTGGCCTT AAAGGAGACC CTGGCTCTAA GGGTGAGAAG 
GGTCACCCTG GTCTTATCGG TCTCATCGGG CCGCCCGGTG AACAGGGAGA GAAGGGAGAT 
CGGGGTTTGC CTGGACCTCA GGGTGCTGCC GGCAGTAAGG GTGATAATGG CATCAGTGGT 
GCTTCTGGTC CAATTGGTCC TCCTGGTCCT CCTGGATTGC CTGGTCCACA AGGGCCAAAG 
GGATCCAAGG GTTCAACTGG TCCTGCTGGT CAAAAAGGAG ACACTGGCAC GGTTGGACCT 
CCTGGGCCTC CTGGTCCTCC TGGTGAGGTC ATCCAGCCTC TGCCCATCCA CAGTCCCAAG 
AAATCCCGTC GCTCCATCGA CATGCAGGCG GATGAAGCAG GCACCATGCT GGACTATGGA 
GAGGGCATGG AGGACATATT CAGCTCTCTC AATAATCTCA AACAGGACAT CGAGCGCATG 
AAGTACCCCA TGGGCACGCA AAACAACCCA GCACGAAGCT GTAAAGACCT TCAGCTCGCT 
CACCCTGAAT TCCCAGATGG CGAATATTAC ATTGATCCTA ACCAGGGCTG CTCTGGAGAC 
TCCTTCAAAG TGTACTGCAA CTTCACAGCA GGAGGAGAGA CCTGCATCTT CCCTGATAAA 
AAGTCAAATG GAGTGAGAAT ATCTTCATGG CCCAAAGAGG TGCCCGGCTC CTGGTTCAGT 
GAATTTAAGC GTGGCAAAAT ACTTTCATAC GTGGACGCAG AAGGCAACTC GATAAACATG 
GTCCAGATGA CGTTCCTCAA ACTACTGACA GCCTCCGCCA GGCAAAACCT GACCTACAGC 
TGCCACCAGT CAGTGGCCTG GCACGACGCC ACTACGGACA GCTATGACAG AGCATTACAC 
TTCCTCGGCT CCAACGACGA GGAGATCTCA TACGACAACA ACCCCTACAT CAAGGCCCTG 
TCAGACGGCT GTGCGGTGAG GAAGGGCTAT GGAAAGACAG TACTGGAGAT TAACACTCCC 
AAAATTGATC AGGTTCCAAT CGTCGACGTG AAGTTCACTG ACTTTGGGGA TCCCAACCAG 
AAATTTGGAT TTGAAGTTGG TCCAGTCTGC TTCCTTGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162871.1
CDS	282..5723
Translation	MERPRWSPMWKTKRWLLDSTVFKLITVSLVLVLQAPHVRAEPVDVLKVLEFPTVPEGVEKTSGFCTNRRASQPDAAYKISKNAQLSAPTKQLFPDGVFPEDFSILTTIKPKAGIQSFLLSIYNEKGVQQLGVEVGRSPVFLYEDQEGKPAPEDYPLFRALNLADGKWHRVGISVEKKTVTIVVDCKKKLTKPLGRSDHASIDTKGITVFGTRILDEESFEGDIQQLLIVADPKAAYDYCEHYSPDCDVPHKDTLQAQEPGEEYTTPVEYGDLYDYYEGGTPTATDEFSEPQSNNVDGEYYTDSELDYGTVDASKTPVATTGPNKAMEEENFGDYITETAQAGLDPTAASEAVLPSGTADPIGVDSYDFKEYDLKEYDVTGLDKNQYEYGPYDEFGNVAPNPTSNYEDTFGPGVAAETDIAETDLGGGAALGQKGEKGEPAVIEPGMLIEGPSGPPGPAGLPGPPGLQGPPGLAGDPGDRGPPGRAGLPGADGLPGPPGTMLMLPFRFGGDGEKGPVVSAQEAQAQAILSQARLAMRGPPGPMGLTGRSGPVGLPGPPGLKGESGDHGPQGPRGIQGPPGQIGKPGKRGRAGADGARGMPGESGSKGDRGFDGLPGLPGEKGHRGETGPAGPPGPTGEDGPRGEDGEIGPRGLAGESGPRGLLGPRGPPGPPGQPGIAGVDGPQGPKGNMGPQGEPGPPGQQGIPGGQGLPGPQGPIGPPGEKGPQGRSGLPGLPGADGPPGHPGKEGPPGEKGALGQPGPQGPIGYPGPRGVKGADGVRGLKGGKGEKGEDGFPGFKGDMGLKGDRGELGLAGPRGEDGPEGPKGRAGPNGESGPQGSAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGGRGVAGKAGPRGQRGPTGPRGARGARGPTGKPGPKGTAGNDGPPGPPGERGPQGPQGPVGFPGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPGGVVGPQGPTGETGPIGERGHPGPPGPPGEQGLPGAGGKEGAKGDPGPQGSPGKDGPPGLRGFPGERGLPGVTGPPGLKGAEGPQGPPGPVGSPGERGAAGSAGPIGLPGRTGPQGPPGPAGEKGAPGERGPLGPAGRDGVQGPVGLPGPAGPQGPPGEDGDKGEVGEPGQKGSKADKGEQGPPGPPGLQGPIGAPGPAGADGEPGPRGQQGMFGQKGDEGSRGFPGPPGPIGLQGLPGPPGEKGENGDVGPMGPPGPPGPRGPQGPPGADGPQGPPGGIGGMGAVGEKGEQGEAGNPGPPGEPGPGGPKGERGEKGEAGPPGAAGPAGPKGPPGDDGPKGNPGPVGFPGDPGPPGEPGVAGIDGGPGEKGEDGESGQPGPPGPSGEAGPPGPPGKRGPPGAHGAEGRQGEKGAKGEAGLEGPPGKTGPVGPQGPSGKPGPEGLRGIPGPVGEQGLPGAPGQDGPPGPMGPPGLPGLKGDPGSKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGAAGSKGDNGISGASGPIGPPGPPGLPGPQGPKGSKGSTGPAGQKGDTGTVGPPGPPGPPGEVIQPLPIHSPKKSRRSIDMQADEAGTMLDYGEGMEDIFSSLNNLKQDIERMKYPMGTQNNPARSCKDLQLAHPEFPDGEYYIDPNQGCSGDSFKVYCNFTAGGETCIFPDKKSNGVRISSWPKEVPGSWFSEFKRGKILSYVDAEGNSINMVQMTFLKLLTASARQNLTYSCHQSVAWHDATTDSYDRALHFLGSNDEEISYDNNPYIKALSDGCAVRKGYGKTVLEINTPKIDQVPIVDVKFTDFGDPNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	XM_005162814.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XI, alpha 1a (col11a1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162814.1

ORF Insert Sequence:

ATGGAGAGGC CAAGGTGGTC CCCAATGTGG AAAACGAAAA GGTGGCTTTT GGATTCCACT 
GTTTTCAAAT TGATCACAGT TTCCCTCGTC CTGGTTCTGC AGGCTCCACA TGTCAGAGCA 
GAACCCGTGG ATGTCTTAAA AGTATTAGAA TTTCCCACTG TCCCAGAAGG AGTAGAGAAA 
ACGTCAGGAT TCTGCACAAA CCGAAGAGCA TCACAGCCAG ACGCGGCCTA CAAAATCAGC 
AAGAATGCCC AGCTCAGTGC CCCGACCAAG CAGCTTTTCC CAGATGGCGT TTTCCCTGAA 
GACTTCTCCA TCCTAACCAC GATCAAGCCC AAAGCAGGGA TCCAGTCCTT CCTCCTGTCC 
ATCTACAATG AGAAAGGCGT CCAGCAGCTG GGCGTGGAGG TGGGCCGTTC ACCTGTCTTC 
CTGTACGAGG ATCAGGAGGG CAAACCTGCC CCGGAGGATT ACCCTCTGTT CCGCGCTCTC 
AATCTGGCCG ACGGAAAGTG GCATCGCGTA GGCATCAGCG TTGAGAAGAA GACAGTGACT 
ATTGTTGTCG ACTGTAAGAA GAAGCTGACC AAGCCATTGG GCAGGAGTGA CCATGCCAGT 
ATCGACACCA AGGGTATCAC CGTTTTTGGC ACCAGGATCC TGGATGAGGA ATCTTTTGAG 
GGTGATATAC AACAACTGCT GATTGTGGCT GACCCTAAAG CAGCTTACGA CTACTGTGAA 
CATTACAGCC CAGACTGTGA CGTCCCTCAC AAGGACACAC TGCAGGCACA GGAGCCCGGA 
GAGGAGTACA CAACTCCTGT TGAATATGGT GATTTATATG ATTATTACGA GGGAGGCACA 
CCCACAGCGA CTGATGAGTT TTCTGAGCCG CAGTCTAACA ATGTAGATGG AGAGTACTAT 
ACAGACAGTG AACTTGACTA CGGAACAGTA GATGCTTCCA AAACGCCTGT CGCAACCACT 
GGACCAAACA AGGCCATGGA AGAGGAGAAT TTTGGTGATT ATATCACCGA GACGGCCCAG 
GCTGGTCTGG ATCCGACCGC TGCCTCAGAA GCTGTGCTGC CCAGTGGCAC CGCTGACCCC 
ATCGGAGTGG ACTCGTATGA CTTTAAGGAG TATGATCTTA AGGAATATGA TGTGACTGGG 
CTTGACAAAA ATCAGTATGA GTATGGGCCT TATGATGAGT TTGGCAATGT GGCTCCAAAC 
CCAACATCCA ATTACGAGGA CACTTTTGGG CCTGGGGTTG CGGCCGAAAC CGACATCGCT 
GAAACAGACC TGGGTGGAGG TGCTGCTCTT GGTCAGAAAG GTGAGAAAGG AGAGCCTGCA 
GTGATTGAAC CAGGAATGCT GATCGAGGGA CCTTCAGGAC CACCTGGACC TGCTGGTCTT 
CCAGGTCCTC CAGGTTTACA AGGCCCCCCT GGTTTGGCTG GAGATCCTGG TGATAGGGGT 
CCTCCAGGCC GTGCTGGTCT TCCTGGAGCT GATGGTTTGC CAGGACCACC TGGAACTATG 
CTCATGCTGC CGTTTCGTTT TGGTGGTGAT GGAGAAAAGG GCCCAGTGGT TTCAGCTCAG 
GAGGCTCAGG CTCAGGCTAT TCTCTCTCAG GCCAGGCTCG CCATGAGAGG TCCACCGGGT 
CCAATGGGTC TCACTGGACG ATCTGGACCA GTGGGTCTTC CAGGTCCTCC TGGACTGAAG 
GGAGAAAGTG GGGATCATGG TCCTCAGGGT CCAAGGGGAA TCCAAGGCCC ACCAGGGCAA 
ATAGGAAAAC CTGGAAAGAG GGGTCGTGCT GGAGCTGATG GTGCCCGAGG AATGCCTGGA 
GAGTCTGGAT CTAAGGGTGA CAGAGGTTTC GATGGGCTTC CGGGTCTGCC TGGTGAGAAA 
GGACACAGGG GTGAGACCGG CCCTGCTGGA CCCCCGGGAC CAACTGGAGA GGATGGACCA 
AGAGGAGAAG ATGGTGAGAT CGGACCAAGA GGACTTGCTG GTGAGAGCGG GCCGCGAGGT 
CTTCTCGGCC CAAGAGGCCC TCCTGGACCT CCTGGACAAC CTGGCATTGC TGGTGTTGAT 
GGTCCGCAGG GTCCCAAAGG AAACATGGGG CCTCAGGGAG AGCCAGGTCC TCCTGGACAG 
CAGGGAATCC CAGGAGGACA GGGTCTTCCA GGTCCACAGG GACCCATCGG ACCACCCGGA 
GAAAAAGGCC CTCAGGGAAG GTCTGGTTTG CCTGGATTGC CTGGTGCAGA TGGGCCTCCT 
GGACATCCTG GTAAAGAGGG TCCACCTGGA GAGAAAGGAG CTTTGGGCCA GCCTGGTCCC 
CAGGGCCCCA TCGGCTATCC TGGCCCTCGC GGTGTGAAGG GTGCAGATGG TGTCCGTGGT 
CTAAAGGGAG GCAAAGGAGA GAAGGGTGAG GATGGATTCC CAGGTTTCAA GGGAGACATG 
GGTCTCAAAG GCGACAGGGG AGAGCTGGGA CTGGCTGGAC CCAGAGGAGA AGATGGTCCA 
GAGGGCCCGA AGGGTCGTGC AGGGCCTAAC GGTGAATCTG GACCCCAGGG ATCTGCCGGA 
GAAAAGGGCA AACTAGGTGT TCCAGGATTG CCCGGCTATC CAGGACGTCA AGGGCCAAAG 
GGTTCGACCG GTTTCCCCGG ATTCCCAGGA GCCAATGGAG AGAAAGGAGG CAGGGGGGTG 
GCTGGGAAAG CTGGTCCAAG AGGCCAAAGA GGTCCAACGG GCCCCCGTGG TGCACGAGGT 
GCAAGAGGCC CAACAGGAAA GCCAGGCCCG AAGGGAACAG CAGGCAACGA CGGTCCACCT 
GGCCCACCCG GTGAGAGAGG CCCTCAAGGA CCCCAGGGTC CTGTTGGTTT CCCAGGACCA 
AAGGGTCCCC CTGGACCACC TGGAAAGGAT GGGCTGCCCG GTCACCCTGG CCAGCGGGGA 
GAGACTGGAT TCCAAGGCAA AACTGGCCCA CCAGGACCTG GAGGTGTTGT TGGACCACAA 
GGACCGACTG GAGAAACCGG TCCAATTGGT GAAAGAGGAC ATCCTGGACC CCCTGGTCCC 
CCCGGAGAGC AGGGTCTTCC TGGCGCTGGT GGAAAAGAAG GGGCAAAGGG AGATCCAGGT 
CCTCAGGGCA GTCCAGGAAA AGACGGTCCA CCAGGACTCA GAGGGTTCCC TGGAGAAAGA 
GGTCTTCCAG GAGTGACGGG TCCACCTGGT TTGAAAGGCG CCGAGGGCCC TCAGGGCCCT 
CCTGGCCCTG TTGGTTCCCC TGGTGAGAGA GGAGCAGCAG GATCTGCTGG TCCCATTGGC 
CTGCCTGGAC GTACTGGACC TCAGGGACCC CCAGGACCTG CTGGAGAGAA GGGTGCACCG 
GGAGAGAGAG GTCCTCTGGG TCCAGCCGGT AGGGATGGAG TTCAAGGTCC AGTTGGTCTG 
CCAGGTCCAG CAGGACCTCA GGGTCCACCT GGAGAAGATG GTGACAAGGG AGAAGTGGGT 
GAACCAGGCC AGAAGGGAAG CAAAGCTGAC AAAGGAGAAC AGGGTCCACC AGGTCCTCCT 
GGTCTTCAGG GACCCATTGG AGCTCCTGGA CCTGCTGGAG CAGATGGCGA GCCCGGTCCT 
CGTGGACAGC AGGGTATGTT TGGACAGAAG GGAGATGAAG GATCCCGGGG CTTCCCTGGA 
CCTCCTGGTC CCATCGGTCT GCAGGGTCTT CCAGGACCAC CTGGCGAGAA AGGTGAAAAT 
GGAGATGTTG GTCCTATGGG TCCTCCTGGT CCACCAGGTC CCAGAGGACC TCAGGGTCCT 
CCTGGTGCTG ATGGCCCGCA AGGTCCTCCT GGTGGAATAG GAGGAATGGG AGCCGTGGGT 
GAGAAGGGTG AGCAAGGAGA AGCTGGCAAC CCTGGACCTC CTGGAGAGCC TGGACCTGGG 
GGCCCGAAAG GTGAGAGAGG AGAGAAAGGT GAAGCTGGTC CCCCTGGTGC TGCTGGACCT 
GCAGGTCCCA AAGGACCCCC TGGAGATGAT GGACCCAAGG GTAACCCTGG CCCTGTTGGT 
TTTCCTGGAG ACCCAGGTCC ACCTGGTGAG CCAGGTGTTG CTGGAATAGA TGGAGGCCCA 
GGAGAGAAGG GAGAGGATGG AGAGTCTGGT CAGCCTGGAC CCCCTGGTCC TTCTGGAGAA 
GCTGGTCCAC CAGGACCTCC TGGAAAACGA GGACCCCCGG GGGCGCATGG AGCTGAAGGC 
AGACAAGGAG AGAAGGGCGC CAAGGGAGAA GCAGGACTTG AGGGTCCACC AGGTAAAACT 
GGACCAGTTG GTCCTCAAGG ACCCTCTGGA AAGCCTGGTC CTGAAGGTCT CAGAGGAATC 
CCCGGTCCAG TCGGTGAACA AGGACTTCCT GGTGCTCCTG GACAAGATGG TCCACCTGGA 
CCAATGGGAC CTCCTGGTCT GCCTGGCCTT AAAGGAGACC CTGGCTCTAA GGGTGAGAAG 
GGTCACCCTG GTCTTATCGG TCTCATCGGG CCGCCCGGTG AACAGGGAGA GAAGGGAGAT 
CGGGGTTTGC CTGGACCTCA GGGTGCTGCC GGCAGTAAGG GTGATAATGG CATCAGTGGT 
GCTTCTGGTC CAATTGGTCC TCCTGGTCCT CCTGGATTGC CTGGTCCACA AGGGCCAAAG 
GGATCCAAGG GTTCAACTGG TCCTGCTGGT CAAAAAGGAG ACACTGGCAC GGTTGGACCT 
CCTGGGCCTC CTGGTCCTCC TGGTGAGGTC ATCCAGCCTC TGCCCATCCA CAGTCCCAAG 
AAATCCCGTC GCTCCATCGA CATGCAGGCG GATGAAGCAG GCACCATGCT GGACTATGGA 
GAGGGCATGG AGGACATATT CAGCTCTCTC AATAATCTCA AACAGGACAT CGAGCGCATG 
AAGTACCCCA TGGGCACGCA AAACAACCCA GCACGAAGCT GTAAAGACCT TCAGCTCGCT 
CACCCTGAAT TCCCAGATGG CGAATATTAC ATTGATCCTA ACCAGGGCTG CTCTGGAGAC 
TCCTTCAAAG TGTACTGCAA CTTCACAGCA GGAGGAGAGA CCTGCATCTT CCCTGATAAA 
AAGTCAAATG GAGTGAGAAT ATCTTCATGG CCCAAAGAGG TGCCCGGCTC CTGGTTCAGT 
GAATTTAAGC GTGGCAAAAT ACTTTCATAC GTGGACGCAG AAGGCAACTC GATAAACATG 
GTCCAGATGA CGTTCCTCAA ACTACTGACA GCCTCCGCCA GGCAAAACCT GACCTACAGC 
TGCCACCAGT CAGTGGCCTG GCACGACGCC ACTACGGACA GCTATGACAG AGCATTACAC 
TTCCTCGGCT CCAACGACGA GGAGATCTCA TACGACAACA ACCCCTACAT CAAGGCCCTG 
TCAGACGGCT GTGCGGTGAG GAAGGGCTAT GGAAAGACAG TACTGGAGAT TAACACTCCC 
AAAATTGATC AGGTTCCAAT CGTCGACGTG AAGTTCACTG ACTTTGGGGA TCCCAACCAG 
AAATTTGGAT TTGAAGTTGG TCCAGTCTGC TTCCTTGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col11a1a ( XM_005162815.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162872.1 Danio rerio collagen, type XI, alpha 1a (col11a1a), transcript variant X3, mRNA.

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CloneID	ODa28709
Clone ID Related Accession (Same CDS sequence)	XM_005162815.1
Accession Version	XM_005162815.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-1(XI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGGC CAAGGTGGTC CCCAATGTGG AAAACGAAAA GGTGGCTTTT GGATTCCACT 
GTTTTCAAAT TGATCACAGT TTCCCTCGTC CTGGTTCTGC AGGCTCCACA TGTCAGAGCA 
GCAGAACCCG TGGATGTCTT AAAAGTATTA GAATTTCCCA CTGTCCCAGA AGGAGTAGAG 
AAAACGTCAG GATTCTGCAC AAACCGAAGA GCATCACAGC CAGACGCGGC CTACAAAATC 
AGCAAGAATG CCCAGCTCAG TGCCCCGACC AAGCAGCTTT TCCCAGATGG CGTTTTCCCT 
GAAGACTTCT CCATCCTAAC CACGATCAAG CCCAAAGCAG GGATCCAGTC CTTCCTCCTG 
TCCATCTACA ATGAGAAAGG CGTCCAGCAG CTGGGCGTGG AGGTGGGCCG TTCACCTGTC 
TTCCTGTACG AGGATCAGGA GGGCAAACCT GCCCCGGAGG ATTACCCTCT GTTCCGCGCT 
CTCAATCTGG CCGACGGAAA GTGGCATCGC GTAGGCATCA GCGTTGAGAA GAAGACAGTG 
ACTATTGTTG TCGACTGTAA GAAGAAGCTG ACCAAGCCAT TGGGCAGGAG TGACCATGCC 
AGTATCGACA CCAAGGGTAT CACCGTTTTT GGCACCAGGA TCCTGGATGA GGAATCTTTT 
GAGGGTGATA TACAACAACT GCTGATTGTG GCTGACCCTA AAGCAGCTTA CGACTACTGT 
GAACATTACA GCCCAGACTG TGACGTCCCT CACAAGGACA CACTGCAGGC ACAGGAGCCC 
GGAGAGGAGT CTAACAATGT AGATGGAGAG TACTATACAG ACAGTGAACT TGACTACGGA 
ACAGTAGATG CTTCCAAAAC GCCTGTCGCA ACCACTGGAC CAAACAAGGC CATGGAAGAG 
GAGAATTTTG GTGATTATAT CACCGAGACG GCCCAGGCTG GTCTGGATCC GACCGCTGCC 
TCAGAAGCTG TGCTGCCCAG TGGCACCGCT GACCCCATCG GAGTGGACTC GTATGACTTT 
AAGGAGTATG ATCTTAAGGA ATATGATGTG ACTGGGCTTG ACAAAAATCA GTATGAGTAT 
GGGCCTTATG ATGAGTTTGG CAATGTGGCT CCAAACCCAA CATCCAATTA CGAGGACACT 
TTTGGGCCTG GGGTTGCGGC CGAAACCGAC ATCGCTGAAA CAGACCTGGG TGGAGGTGCT 
GCTCTTGGTC AGAAAGGTGA GAAAGGAGAG CCTGCAGTGA TTGAACCAGG AATGCTGATC 
GAGGGACCTT CAGGACCACC TGGACCTGCT GGTCTTCCAG GTCCTCCAGG TTTACAAGGC 
CCCCCTGGTT TGGCTGGAGA TCCTGGTGAT AGGGGTCCTC CAGGCCGTGC TGGTCTTCCT 
GGAGCTGATG GTTTGCCAGG ACCACCTGGA ACTATGCTCA TGCTGCCGTT TCGTTTTGGT 
GGTGATGGAG AAAAGGGCCC AGTGGTTTCA GCTCAGGAGG CTCAGGCTCA GGCTATTCTC 
TCTCAGGCCA GGCTCGCCAT GAGAGGTCCA CCGGGTCCAA TGGGTCTCAC TGGACGATCT 
GGACCAGTGG GTCTTCCAGG TCCTCCTGGA CTGAAGGGAG AAAGTGGGGA TCATGGTCCT 
CAGGGTCCAA GGGGAATCCA AGGCCCACCA GGGCAAATAG GAAAACCTGG AAAGAGGGGT 
CGTGCTGGAG CTGATGGTGC CCGAGGAATG CCTGGAGAGT CTGGATCTAA GGGTGACAGA 
GGTTTCGATG GGCTTCCGGG TCTGCCTGGT GAGAAAGGAC ACAGGGGTGA GACCGGCCCT 
GCTGGACCCC CGGGACCAAC TGGAGAGGAT GGACCAAGAG GAGAAGATGG TGAGATCGGA 
CCAAGAGGAC TTGCTGGTGA GAGCGGGCCG CGAGGTCTTC TCGGCCCAAG AGGCCCTCCT 
GGACCTCCTG GACAACCTGG CATTGCTGGT GTTGATGGTC CGCAGGGTCC CAAAGGAAAC 
ATGGGGCCTC AGGGAGAGCC AGGTCCTCCT GGACAGCAGG GAATCCCAGG AGGACAGGGT 
CTTCCAGGTC CACAGGGACC CATCGGACCA CCCGGAGAAA AAGGCCCTCA GGGAAGGTCT 
GGTTTGCCTG GATTGCCTGG TGCAGATGGG CCTCCTGGAC ATCCTGGTAA AGAGGGTCCA 
CCTGGAGAGA AAGGAGCTTT GGGCCAGCCT GGTCCCCAGG GCCCCATCGG CTATCCTGGC 
CCTCGCGGTG TGAAGGGTGC AGATGGTGTC CGTGGTCTAA AGGGAGGCAA AGGAGAGAAG 
GGTGAGGATG GATTCCCAGG TTTCAAGGGA GACATGGGTC TCAAAGGCGA CAGGGGAGAG 
CTGGGACTGG CTGGACCCAG AGGAGAAGAT GGTCCAGAGG GCCCGAAGGG TCGTGCAGGG 
CCTAACGGTG AATCTGGACC CCAGGGATCT GCCGGAGAAA AGGGCAAACT AGGTGTTCCA 
GGATTGCCCG GCTATCCAGG ACGTCAAGGG CCAAAGGGTT CGACCGGTTT CCCCGGATTC 
CCAGGAGCCA ATGGAGAGAA AGGAGGCAGG GGGGTGGCTG GGAAAGCTGG TCCAAGAGGC 
CAAAGAGGTC CAACGGGCCC CCGTGGTGCA CGAGGTGCAA GAGGCCCAAC AGGAAAGCCA 
GGCCCGAAGG GAACAGCAGG CAACGACGGT CCACCTGGCC CACCCGGTGA GAGAGGCCCT 
CAAGGACCCC AGGGTCCTGT TGGTTTCCCA GGACCAAAGG GTCCCCCTGG ACCACCTGGA 
AAGGATGGGC TGCCCGGTCA CCCTGGCCAG CGGGGAGAGA CTGGATTCCA AGGCAAAACT 
GGCCCACCAG GACCTGGAGG TGTTGTTGGA CCACAAGGAC CGACTGGAGA AACCGGTCCA 
ATTGGTGAAA GAGGACATCC TGGACCCCCT GGTCCCCCCG GAGAGCAGGG TCTTCCTGGC 
GCTGGTGGAA AAGAAGGGGC AAAGGGAGAT CCAGGTCCTC AGGGCAGTCC AGGAAAAGAC 
GGTCCACCAG GACTCAGAGG GTTCCCTGGA GAAAGAGGTC TTCCAGGAGT GACGGGTCCA 
CCTGGTTTGA AAGGCGCCGA GGGCCCTCAG GGCCCTCCTG GCCCTGTTGG TTCCCCTGGT 
GAGAGAGGAG CAGCAGGATC TGCTGGTCCC ATTGGCCTGC CTGGACGTAC TGGACCTCAG 
GGACCCCCAG GACCTGCTGG AGAGAAGGGT GCACCGGGAG AGAGAGGTCC TCTGGGTCCA 
GCCGGTAGGG ATGGAGTTCA AGGTCCAGTT GGTCTGCCAG GTCCAGCAGG ACCTCAGGGT 
CCACCTGGAG AAGATGGTGA CAAGGGAGAA GTGGGTGAAC CAGGCCAGAA GGGAAGCAAA 
GCTGACAAAG GAGAACAGGG TCCACCAGGT CCTCCTGGTC TTCAGGGACC CATTGGAGCT 
CCTGGACCTG CTGGAGCAGA TGGCGAGCCC GGTCCTCGTG GACAGCAGGG TATGTTTGGA 
CAGAAGGGAG ATGAAGGATC CCGGGGCTTC CCTGGACCTC CTGGTCCCAT CGGTCTGCAG 
GGTCTTCCAG GACCACCTGG CGAGAAAGGT GAAAATGGAG ATGTTGGTCC TATGGGTCCT 
CCTGGTCCAC CAGGTCCCAG AGGACCTCAG GGTCCTCCTG GTGCTGATGG CCCGCAAGGT 
CCTCCTGGTG GAATAGGAGG AATGGGAGCC GTGGGTGAGA AGGGTGAGCA AGGAGAAGCT 
GGCAACCCTG GACCTCCTGG AGAGCCTGGA CCTGGGGGCC CGAAAGGTGA GAGAGGAGAG 
AAAGGTGAAG CTGGTCCCCC TGGTGCTGCT GGACCTGCAG GTCCCAAAGG ACCCCCTGGA 
GATGATGGAC CCAAGGGTAA CCCTGGCCCT GTTGGTTTTC CTGGAGACCC AGGTCCACCT 
GGTGAGCCAG GTGTTGCTGG AATAGATGGA GGCCCAGGAG AGAAGGGAGA GGATGGAGAG 
TCTGGTCAGC CTGGACCCCC TGGTCCTTCT GGAGAAGCTG GTCCACCAGG ACCTCCTGGA 
AAACGAGGAC CCCCGGGGGC GCATGGAGCT GAAGGCAGAC AAGGAGAGAA GGGCGCCAAG 
GGAGAAGCAG GACTTGAGGG TCCACCAGGT AAAACTGGAC CAGTTGGTCC TCAAGGACCC 
TCTGGAAAGC CTGGTCCTGA AGGTCTCAGA GGAATCCCCG GTCCAGTCGG TGAACAAGGA 
CTTCCTGGTG CTCCTGGACA AGATGGTCCA CCTGGACCAA TGGGACCTCC TGGTCTGCCT 
GGCCTTAAAG GAGACCCTGG CTCTAAGGGT GAGAAGGGTC ACCCTGGTCT TATCGGTCTC 
ATCGGGCCGC CCGGTGAACA GGGAGAGAAG GGAGATCGGG GTTTGCCTGG ACCTCAGGGT 
GCTGCCGGCA GTAAGGGTGA TAATGGCATC AGTGGTGCTT CTGGTCCAAT TGGTCCTCCT 
GGTCCTCCTG GATTGCCTGG TCCACAAGGG CCAAAGGGAT CCAAGGGTTC AACTGGTCCT 
GCTGGTCAAA AAGGAGACAC TGGCACGGTT GGACCTCCTG GGCCTCCTGG TCCTCCTGGT 
GAGGTCATCC AGCCTCTGCC CATCCACAGT CCCAAGAAAT CCCGTCGCTC CATCGACATG 
CAGGCGGATG AAGCAGGCAC CATGCTGGAC TATGGAGAGG GCATGGAGGA CATATTCAGC 
TCTCTCAATA ATCTCAAACA GGACATCGAG CGCATGAAGT ACCCCATGGG CACGCAAAAC 
AACCCAGCAC GAAGCTGTAA AGACCTTCAG CTCGCTCACC CTGAATTCCC AGATGGCGAA 
TATTACATTG ATCCTAACCA GGGCTGCTCT GGAGACTCCT TCAAAGTGTA CTGCAACTTC 
ACAGCAGGAG GAGAGACCTG CATCTTCCCT GATAAAAAGT CAAATGGAGT GAGAATATCT 
TCATGGCCCA AAGAGGTGCC CGGCTCCTGG TTCAGTGAAT TTAAGCGTGG CAAAATACTT 
TCATACGTGG ACGCAGAAGG CAACTCGATA AACATGGTCC AGATGACGTT CCTCAAACTA 
CTGACAGCCT CCGCCAGGCA AAACCTGACC TACAGCTGCC ACCAGTCAGT GGCCTGGCAC 
GACGCCACTA CGGACAGCTA TGACAGAGCA TTACACTTCC TCGGCTCCAA CGACGAGGAG 
ATCTCATACG ACAACAACCC CTACATCAAG GCCCTGTCAG ACGGCTGTGC GGTGAGGAAG 
GGCTATGGAA AGACAGTACT GGAGATTAAC ACTCCCAAAA TTGATCAGGT TCCAATCGTC 
GACGTGAAGT TCACTGACTT TGGGGATCCC AACCAGAAAT TTGGATTTGA AGTTGGTCCA 
GTCTGCTTCC TTGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162872.1
CDS	282..5639
Translation	MERPRWSPMWKTKRWLLDSTVFKLITVSLVLVLQAPHVRAAEPVDVLKVLEFPTVPEGVEKTSGFCTNRRASQPDAAYKISKNAQLSAPTKQLFPDGVFPEDFSILTTIKPKAGIQSFLLSIYNEKGVQQLGVEVGRSPVFLYEDQEGKPAPEDYPLFRALNLADGKWHRVGISVEKKTVTIVVDCKKKLTKPLGRSDHASIDTKGITVFGTRILDEESFEGDIQQLLIVADPKAAYDYCEHYSPDCDVPHKDTLQAQEPGEESNNVDGEYYTDSELDYGTVDASKTPVATTGPNKAMEEENFGDYITETAQAGLDPTAASEAVLPSGTADPIGVDSYDFKEYDLKEYDVTGLDKNQYEYGPYDEFGNVAPNPTSNYEDTFGPGVAAETDIAETDLGGGAALGQKGEKGEPAVIEPGMLIEGPSGPPGPAGLPGPPGLQGPPGLAGDPGDRGPPGRAGLPGADGLPGPPGTMLMLPFRFGGDGEKGPVVSAQEAQAQAILSQARLAMRGPPGPMGLTGRSGPVGLPGPPGLKGESGDHGPQGPRGIQGPPGQIGKPGKRGRAGADGARGMPGESGSKGDRGFDGLPGLPGEKGHRGETGPAGPPGPTGEDGPRGEDGEIGPRGLAGESGPRGLLGPRGPPGPPGQPGIAGVDGPQGPKGNMGPQGEPGPPGQQGIPGGQGLPGPQGPIGPPGEKGPQGRSGLPGLPGADGPPGHPGKEGPPGEKGALGQPGPQGPIGYPGPRGVKGADGVRGLKGGKGEKGEDGFPGFKGDMGLKGDRGELGLAGPRGEDGPEGPKGRAGPNGESGPQGSAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGGRGVAGKAGPRGQRGPTGPRGARGARGPTGKPGPKGTAGNDGPPGPPGERGPQGPQGPVGFPGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPGGVVGPQGPTGETGPIGERGHPGPPGPPGEQGLPGAGGKEGAKGDPGPQGSPGKDGPPGLRGFPGERGLPGVTGPPGLKGAEGPQGPPGPVGSPGERGAAGSAGPIGLPGRTGPQGPPGPAGEKGAPGERGPLGPAGRDGVQGPVGLPGPAGPQGPPGEDGDKGEVGEPGQKGSKADKGEQGPPGPPGLQGPIGAPGPAGADGEPGPRGQQGMFGQKGDEGSRGFPGPPGPIGLQGLPGPPGEKGENGDVGPMGPPGPPGPRGPQGPPGADGPQGPPGGIGGMGAVGEKGEQGEAGNPGPPGEPGPGGPKGERGEKGEAGPPGAAGPAGPKGPPGDDGPKGNPGPVGFPGDPGPPGEPGVAGIDGGPGEKGEDGESGQPGPPGPSGEAGPPGPPGKRGPPGAHGAEGRQGEKGAKGEAGLEGPPGKTGPVGPQGPSGKPGPEGLRGIPGPVGEQGLPGAPGQDGPPGPMGPPGLPGLKGDPGSKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGAAGSKGDNGISGASGPIGPPGPPGLPGPQGPKGSKGSTGPAGQKGDTGTVGPPGPPGPPGEVIQPLPIHSPKKSRRSIDMQADEAGTMLDYGEGMEDIFSSLNNLKQDIERMKYPMGTQNNPARSCKDLQLAHPEFPDGEYYIDPNQGCSGDSFKVYCNFTAGGETCIFPDKKSNGVRISSWPKEVPGSWFSEFKRGKILSYVDAEGNSINMVQMTFLKLLTASARQNLTYSCHQSVAWHDATTDSYDRALHFLGSNDEEISYDNNPYIKALSDGCAVRKGYGKTVLEINTPKIDQVPIVDVKFTDFGDPNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	XM_005162815.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XI, alpha 1a (col11a1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162815.1

ORF Insert Sequence:

ATGGAGAGGC CAAGGTGGTC CCCAATGTGG AAAACGAAAA GGTGGCTTTT GGATTCCACT 
GTTTTCAAAT TGATCACAGT TTCCCTCGTC CTGGTTCTGC AGGCTCCACA TGTCAGAGCA 
GCAGAACCCG TGGATGTCTT AAAAGTATTA GAATTTCCCA CTGTCCCAGA AGGAGTAGAG 
AAAACGTCAG GATTCTGCAC AAACCGAAGA GCATCACAGC CAGACGCGGC CTACAAAATC 
AGCAAGAATG CCCAGCTCAG TGCCCCGACC AAGCAGCTTT TCCCAGATGG CGTTTTCCCT 
GAAGACTTCT CCATCCTAAC CACGATCAAG CCCAAAGCAG GGATCCAGTC CTTCCTCCTG 
TCCATCTACA ATGAGAAAGG CGTCCAGCAG CTGGGCGTGG AGGTGGGCCG TTCACCTGTC 
TTCCTGTACG AGGATCAGGA GGGCAAACCT GCCCCGGAGG ATTACCCTCT GTTCCGCGCT 
CTCAATCTGG CCGACGGAAA GTGGCATCGC GTAGGCATCA GCGTTGAGAA GAAGACAGTG 
ACTATTGTTG TCGACTGTAA GAAGAAGCTG ACCAAGCCAT TGGGCAGGAG TGACCATGCC 
AGTATCGACA CCAAGGGTAT CACCGTTTTT GGCACCAGGA TCCTGGATGA GGAATCTTTT 
GAGGGTGATA TACAACAACT GCTGATTGTG GCTGACCCTA AAGCAGCTTA CGACTACTGT 
GAACATTACA GCCCAGACTG TGACGTCCCT CACAAGGACA CACTGCAGGC ACAGGAGCCC 
GGAGAGGAGT CTAACAATGT AGATGGAGAG TACTATACAG ACAGTGAACT TGACTACGGA 
ACAGTAGATG CTTCCAAAAC GCCTGTCGCA ACCACTGGAC CAAACAAGGC CATGGAAGAG 
GAGAATTTTG GTGATTATAT CACCGAGACG GCCCAGGCTG GTCTGGATCC GACCGCTGCC 
TCAGAAGCTG TGCTGCCCAG TGGCACCGCT GACCCCATCG GAGTGGACTC GTATGACTTT 
AAGGAGTATG ATCTTAAGGA ATATGATGTG ACTGGGCTTG ACAAAAATCA GTATGAGTAT 
GGGCCTTATG ATGAGTTTGG CAATGTGGCT CCAAACCCAA CATCCAATTA CGAGGACACT 
TTTGGGCCTG GGGTTGCGGC CGAAACCGAC ATCGCTGAAA CAGACCTGGG TGGAGGTGCT 
GCTCTTGGTC AGAAAGGTGA GAAAGGAGAG CCTGCAGTGA TTGAACCAGG AATGCTGATC 
GAGGGACCTT CAGGACCACC TGGACCTGCT GGTCTTCCAG GTCCTCCAGG TTTACAAGGC 
CCCCCTGGTT TGGCTGGAGA TCCTGGTGAT AGGGGTCCTC CAGGCCGTGC TGGTCTTCCT 
GGAGCTGATG GTTTGCCAGG ACCACCTGGA ACTATGCTCA TGCTGCCGTT TCGTTTTGGT 
GGTGATGGAG AAAAGGGCCC AGTGGTTTCA GCTCAGGAGG CTCAGGCTCA GGCTATTCTC 
TCTCAGGCCA GGCTCGCCAT GAGAGGTCCA CCGGGTCCAA TGGGTCTCAC TGGACGATCT 
GGACCAGTGG GTCTTCCAGG TCCTCCTGGA CTGAAGGGAG AAAGTGGGGA TCATGGTCCT 
CAGGGTCCAA GGGGAATCCA AGGCCCACCA GGGCAAATAG GAAAACCTGG AAAGAGGGGT 
CGTGCTGGAG CTGATGGTGC CCGAGGAATG CCTGGAGAGT CTGGATCTAA GGGTGACAGA 
GGTTTCGATG GGCTTCCGGG TCTGCCTGGT GAGAAAGGAC ACAGGGGTGA GACCGGCCCT 
GCTGGACCCC CGGGACCAAC TGGAGAGGAT GGACCAAGAG GAGAAGATGG TGAGATCGGA 
CCAAGAGGAC TTGCTGGTGA GAGCGGGCCG CGAGGTCTTC TCGGCCCAAG AGGCCCTCCT 
GGACCTCCTG GACAACCTGG CATTGCTGGT GTTGATGGTC CGCAGGGTCC CAAAGGAAAC 
ATGGGGCCTC AGGGAGAGCC AGGTCCTCCT GGACAGCAGG GAATCCCAGG AGGACAGGGT 
CTTCCAGGTC CACAGGGACC CATCGGACCA CCCGGAGAAA AAGGCCCTCA GGGAAGGTCT 
GGTTTGCCTG GATTGCCTGG TGCAGATGGG CCTCCTGGAC ATCCTGGTAA AGAGGGTCCA 
CCTGGAGAGA AAGGAGCTTT GGGCCAGCCT GGTCCCCAGG GCCCCATCGG CTATCCTGGC 
CCTCGCGGTG TGAAGGGTGC AGATGGTGTC CGTGGTCTAA AGGGAGGCAA AGGAGAGAAG 
GGTGAGGATG GATTCCCAGG TTTCAAGGGA GACATGGGTC TCAAAGGCGA CAGGGGAGAG 
CTGGGACTGG CTGGACCCAG AGGAGAAGAT GGTCCAGAGG GCCCGAAGGG TCGTGCAGGG 
CCTAACGGTG AATCTGGACC CCAGGGATCT GCCGGAGAAA AGGGCAAACT AGGTGTTCCA 
GGATTGCCCG GCTATCCAGG ACGTCAAGGG CCAAAGGGTT CGACCGGTTT CCCCGGATTC 
CCAGGAGCCA ATGGAGAGAA AGGAGGCAGG GGGGTGGCTG GGAAAGCTGG TCCAAGAGGC 
CAAAGAGGTC CAACGGGCCC CCGTGGTGCA CGAGGTGCAA GAGGCCCAAC AGGAAAGCCA 
GGCCCGAAGG GAACAGCAGG CAACGACGGT CCACCTGGCC CACCCGGTGA GAGAGGCCCT 
CAAGGACCCC AGGGTCCTGT TGGTTTCCCA GGACCAAAGG GTCCCCCTGG ACCACCTGGA 
AAGGATGGGC TGCCCGGTCA CCCTGGCCAG CGGGGAGAGA CTGGATTCCA AGGCAAAACT 
GGCCCACCAG GACCTGGAGG TGTTGTTGGA CCACAAGGAC CGACTGGAGA AACCGGTCCA 
ATTGGTGAAA GAGGACATCC TGGACCCCCT GGTCCCCCCG GAGAGCAGGG TCTTCCTGGC 
GCTGGTGGAA AAGAAGGGGC AAAGGGAGAT CCAGGTCCTC AGGGCAGTCC AGGAAAAGAC 
GGTCCACCAG GACTCAGAGG GTTCCCTGGA GAAAGAGGTC TTCCAGGAGT GACGGGTCCA 
CCTGGTTTGA AAGGCGCCGA GGGCCCTCAG GGCCCTCCTG GCCCTGTTGG TTCCCCTGGT 
GAGAGAGGAG CAGCAGGATC TGCTGGTCCC ATTGGCCTGC CTGGACGTAC TGGACCTCAG 
GGACCCCCAG GACCTGCTGG AGAGAAGGGT GCACCGGGAG AGAGAGGTCC TCTGGGTCCA 
GCCGGTAGGG ATGGAGTTCA AGGTCCAGTT GGTCTGCCAG GTCCAGCAGG ACCTCAGGGT 
CCACCTGGAG AAGATGGTGA CAAGGGAGAA GTGGGTGAAC CAGGCCAGAA GGGAAGCAAA 
GCTGACAAAG GAGAACAGGG TCCACCAGGT CCTCCTGGTC TTCAGGGACC CATTGGAGCT 
CCTGGACCTG CTGGAGCAGA TGGCGAGCCC GGTCCTCGTG GACAGCAGGG TATGTTTGGA 
CAGAAGGGAG ATGAAGGATC CCGGGGCTTC CCTGGACCTC CTGGTCCCAT CGGTCTGCAG 
GGTCTTCCAG GACCACCTGG CGAGAAAGGT GAAAATGGAG ATGTTGGTCC TATGGGTCCT 
CCTGGTCCAC CAGGTCCCAG AGGACCTCAG GGTCCTCCTG GTGCTGATGG CCCGCAAGGT 
CCTCCTGGTG GAATAGGAGG AATGGGAGCC GTGGGTGAGA AGGGTGAGCA AGGAGAAGCT 
GGCAACCCTG GACCTCCTGG AGAGCCTGGA CCTGGGGGCC CGAAAGGTGA GAGAGGAGAG 
AAAGGTGAAG CTGGTCCCCC TGGTGCTGCT GGACCTGCAG GTCCCAAAGG ACCCCCTGGA 
GATGATGGAC CCAAGGGTAA CCCTGGCCCT GTTGGTTTTC CTGGAGACCC AGGTCCACCT 
GGTGAGCCAG GTGTTGCTGG AATAGATGGA GGCCCAGGAG AGAAGGGAGA GGATGGAGAG 
TCTGGTCAGC CTGGACCCCC TGGTCCTTCT GGAGAAGCTG GTCCACCAGG ACCTCCTGGA 
AAACGAGGAC CCCCGGGGGC GCATGGAGCT GAAGGCAGAC AAGGAGAGAA GGGCGCCAAG 
GGAGAAGCAG GACTTGAGGG TCCACCAGGT AAAACTGGAC CAGTTGGTCC TCAAGGACCC 
TCTGGAAAGC CTGGTCCTGA AGGTCTCAGA GGAATCCCCG GTCCAGTCGG TGAACAAGGA 
CTTCCTGGTG CTCCTGGACA AGATGGTCCA CCTGGACCAA TGGGACCTCC TGGTCTGCCT 
GGCCTTAAAG GAGACCCTGG CTCTAAGGGT GAGAAGGGTC ACCCTGGTCT TATCGGTCTC 
ATCGGGCCGC CCGGTGAACA GGGAGAGAAG GGAGATCGGG GTTTGCCTGG ACCTCAGGGT 
GCTGCCGGCA GTAAGGGTGA TAATGGCATC AGTGGTGCTT CTGGTCCAAT TGGTCCTCCT 
GGTCCTCCTG GATTGCCTGG TCCACAAGGG CCAAAGGGAT CCAAGGGTTC AACTGGTCCT 
GCTGGTCAAA AAGGAGACAC TGGCACGGTT GGACCTCCTG GGCCTCCTGG TCCTCCTGGT 
GAGGTCATCC AGCCTCTGCC CATCCACAGT CCCAAGAAAT CCCGTCGCTC CATCGACATG 
CAGGCGGATG AAGCAGGCAC CATGCTGGAC TATGGAGAGG GCATGGAGGA CATATTCAGC 
TCTCTCAATA ATCTCAAACA GGACATCGAG CGCATGAAGT ACCCCATGGG CACGCAAAAC 
AACCCAGCAC GAAGCTGTAA AGACCTTCAG CTCGCTCACC CTGAATTCCC AGATGGCGAA 
TATTACATTG ATCCTAACCA GGGCTGCTCT GGAGACTCCT TCAAAGTGTA CTGCAACTTC 
ACAGCAGGAG GAGAGACCTG CATCTTCCCT GATAAAAAGT CAAATGGAGT GAGAATATCT 
TCATGGCCCA AAGAGGTGCC CGGCTCCTGG TTCAGTGAAT TTAAGCGTGG CAAAATACTT 
TCATACGTGG ACGCAGAAGG CAACTCGATA AACATGGTCC AGATGACGTT CCTCAAACTA 
CTGACAGCCT CCGCCAGGCA AAACCTGACC TACAGCTGCC ACCAGTCAGT GGCCTGGCAC 
GACGCCACTA CGGACAGCTA TGACAGAGCA TTACACTTCC TCGGCTCCAA CGACGAGGAG 
ATCTCATACG ACAACAACCC CTACATCAAG GCCCTGTCAG ACGGCTGTGC GGTGAGGAAG 
GGCTATGGAA AGACAGTACT GGAGATTAAC ACTCCCAAAA TTGATCAGGT TCCAATCGTC 
GACGTGAAGT TCACTGACTT TGGGGATCCC AACCAGAAAT TTGGATTTGA AGTTGGTCCA 
GTCTGCTTCC TTGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col11a1a ( XM_005162818.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162875.1 Danio rerio collagen, type XI, alpha 1a (col11a1a), transcript variant X4, mRNA.

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CloneID	ODa28712
Clone ID Related Accession (Same CDS sequence)	XM_005162818.1
Accession Version	XM_005162818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	collagen alpha-1(XI) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGGC CAAGGTGGTC CCCAATGTGG AAAACGAAAA GGTGGCTTTT GGATTCCACT 
GTTTTCAAAT TGATCACAGT TTCCCTCGTC CTGGTTCTGC AGGCTCCACA TGTCAGAGCA 
GCAGAACCCG TGGATGTCTT AAAAGTATTA GAATTTCCCA CTGTCCCAGA AGGAGTAGAG 
AAAACGTCAG GATTCTGCAC AAACCGAAGA GCATCACAGC CAGACGCGGC CTACAAAATC 
AGCAAGAATG CCCAGCTCAG TGCCCCGACC AAGCAGCTTT TCCCAGATGG CGTTTTCCCT 
GAAGACTTCT CCATCCTAAC CACGATCAAG CCCAAAGCAG GGATCCAGTC CTTCCTCCTG 
TCCATCTACA ATGAGAAAGG CGTCCAGCAG CTGGGCGTGG AGGTGGGCCG TTCACCTGTC 
TTCCTGTACG AGGATCAGGA GGGCAAACCT GCCCCGGAGG ATTACCCTCT GTTCCGCGCT 
CTCAATCTGG CCGACGGAAA GTGGCATCGC GTAGGCATCA GCGTTGAGAA GAAGACAGTG 
ACTATTGTTG TCGACTGTAA GAAGAAGCTG ACCAAGCCAT TGGGCAGGAG TGACCATGCC 
AGTATCGACA CCAAGGGTAT CACCGTTTTT GGCACCAGGA TCCTGGATGA GGAATCTTTT 
GAGGGTGATA TACAACAACT GCTGATTGTG GCTGACCCTA AAGCAGCTTA CGACTACTGT 
GAACATTACA GCCCAGACTG TGACGTCCCT CACAAGGACA CACTGCAGGC ACAGGAGCCC 
GGAGAGGAGT ACACAACTCC TGTTGAATAT GGTGATTTAT ATGATTATTA CGAGGGAGGC 
ACACCCACAG CGACTGATGA GTTTTCTGAG CCGCAGTCTA ACAATGTAGA TGGAGAGTAC 
TATACAGACA GTGAACTTGA CTACGGAACA GTAGATGCTT CCAAAACGCC TGTCGCAACC 
ACTGGACCAA ACAAGCTGGG TGGAGGTGCT GCTCTTGGTC AGAAAGGTGA GAAAGGAGAG 
CCTGCAGTGA TTGAACCAGG AATGCTGATC GAGGGACCTT CAGGACCACC TGGACCTGCT 
GGTCTTCCAG GTCCTCCAGG TTTACAAGGC CCCCCTGGTT TGGCTGGAGA TCCTGGTGAT 
AGGGGTCCTC CAGGCCGTGC TGGTCTTCCT GGAGCTGATG GTTTGCCAGG ACCACCTGGA 
ACTATGCTCA TGCTGCCGTT TCGTTTTGGT GGTGATGGAG AAAAGGGCCC AGTGGTTTCA 
GCTCAGGAGG CTCAGGCTCA GGCTATTCTC TCTCAGGCCA GGCTCGCCAT GAGAGGTCCA 
CCGGGTCCAA TGGGTCTCAC TGGACGATCT GGACCAGTGG GTCTTCCAGG TCCTCCTGGA 
CTGAAGGGAG AAAGTGGGGA TCATGGTCCT CAGGGTCCAA GGGGAATCCA AGGCCCACCA 
GGGCAAATAG GAAAACCTGG AAAGAGGGGT CGTGCTGGAG CTGATGGTGC CCGAGGAATG 
CCTGGAGAGT CTGGATCTAA GGGTGACAGA GGTTTCGATG GGCTTCCGGG TCTGCCTGGT 
GAGAAAGGAC ACAGGGGTGA GACCGGCCCT GCTGGACCCC CGGGACCAAC TGGAGAGGAT 
GGACCAAGAG GAGAAGATGG TGAGATCGGA CCAAGAGGAC TTGCTGGTGA GAGCGGGCCG 
CGAGGTCTTC TCGGCCCAAG AGGCCCTCCT GGACCTCCTG GACAACCTGG CATTGCTGGT 
GTTGATGGTC CGCAGGGTCC CAAAGGAAAC ATGGGGCCTC AGGGAGAGCC AGGTCCTCCT 
GGACAGCAGG GAATCCCAGG AGGACAGGGT CTTCCAGGTC CACAGGGACC CATCGGACCA 
CCCGGAGAAA AAGGCCCTCA GGGAAGGTCT GGTTTGCCTG GATTGCCTGG TGCAGATGGG 
CCTCCTGGAC ATCCTGGTAA AGAGGGTCCA CCTGGAGAGA AAGGAGCTTT GGGCCAGCCT 
GGTCCCCAGG GCCCCATCGG CTATCCTGGC CCTCGCGGTG TGAAGGGTGC AGATGGTGTC 
CGTGGTCTAA AGGGAGGCAA AGGAGAGAAG GGTGAGGATG GATTCCCAGG TTTCAAGGGA 
GACATGGGTC TCAAAGGCGA CAGGGGAGAG CTGGGACTGG CTGGACCCAG AGGAGAAGAT 
GGTCCAGAGG GCCCGAAGGG TCGTGCAGGG CCTAACGGTG AATCTGGACC CCAGGGATCT 
GCCGGAGAAA AGGGCAAACT AGGTGTTCCA GGATTGCCCG GCTATCCAGG ACGTCAAGGG 
CCAAAGGGTT CGACCGGTTT CCCCGGATTC CCAGGAGCCA ATGGAGAGAA AGGAGGCAGG 
GGGGTGGCTG GGAAAGCTGG TCCAAGAGGC CAAAGAGGTC CAACGGGCCC CCGTGGTGCA 
CGAGGTGCAA GAGGCCCAAC AGGAAAGCCA GGCCCGAAGG GAACAGCAGG CAACGACGGT 
CCACCTGGCC CACCCGGTGA GAGAGGCCCT CAAGGACCCC AGGGTCCTGT TGGTTTCCCA 
GGACCAAAGG GTCCCCCTGG ACCACCTGGA AAGGATGGGC TGCCCGGTCA CCCTGGCCAG 
CGGGGAGAGA CTGGATTCCA AGGCAAAACT GGCCCACCAG GACCTGGAGG TGTTGTTGGA 
CCACAAGGAC CGACTGGAGA AACCGGTCCA ATTGGTGAAA GAGGACATCC TGGACCCCCT 
GGTCCCCCCG GAGAGCAGGG TCTTCCTGGC GCTGGTGGAA AAGAAGGGGC AAAGGGAGAT 
CCAGGTCCTC AGGGCAGTCC AGGAAAAGAC GGTCCACCAG GACTCAGAGG GTTCCCTGGA 
GAAAGAGGTC TTCCAGGAGT GACGGGTCCA CCTGGTTTGA AAGGCGCCGA GGGCCCTCAG 
GGCCCTCCTG GCCCTGTTGG TTCCCCTGGT GAGAGAGGAG CAGCAGGATC TGCTGGTCCC 
ATTGGCCTGC CTGGACGTAC TGGACCTCAG GGACCCCCAG GACCTGCTGG AGAGAAGGGT 
GCACCGGGAG AGAGAGGTCC TCTGGGTCCA GCCGGTAGGG ATGGAGTTCA AGGTCCAGTT 
GGTCTGCCAG GTCCAGCAGG ACCTCAGGGT CCACCTGGAG AAGATGGTGA CAAGGGAGAA 
GTGGGTGAAC CAGGCCAGAA GGGAAGCAAA GCTGACAAAG GAGAACAGGG TCCACCAGGT 
CCTCCTGGTC TTCAGGGACC CATTGGAGCT CCTGGACCTG CTGGAGCAGA TGGCGAGCCC 
GGTCCTCGTG GACAGCAGGG TATGTTTGGA CAGAAGGGAG ATGAAGGATC CCGGGGCTTC 
CCTGGACCTC CTGGTCCCAT CGGTCTGCAG GGTCTTCCAG GACCACCTGG CGAGAAAGGT 
GAAAATGGAG ATGTTGGTCC TATGGGTCCT CCTGGTCCAC CAGGTCCCAG AGGACCTCAG 
GGTCCTCCTG GTGCTGATGG CCCGCAAGGT CCTCCTGGTG GAATAGGAGG AATGGGAGCC 
GTGGGTGAGA AGGGTGAGCA AGGAGAAGCT GGCAACCCTG GACCTCCTGG AGAGCCTGGA 
CCTGGGGGCC CGAAAGGTGA GAGAGGAGAG AAAGGTGAAG CTGGTCCCCC TGGTGCTGCT 
GGACCTGCAG GTCCCAAAGG ACCCCCTGGA GATGATGGAC CCAAGGGTAA CCCTGGCCCT 
GTTGGTTTTC CTGGAGACCC AGGTCCACCT GGTGAGCCAG GTGTTGCTGG AATAGATGGA 
GGCCCAGGAG AGAAGGGAGA GGATGGAGAG TCTGGTCAGC CTGGACCCCC TGGTCCTTCT 
GGAGAAGCTG GTCCACCAGG ACCTCCTGGA AAACGAGGAC CCCCGGGGGC GCATGGAGCT 
GAAGGCAGAC AAGGAGAGAA GGGCGCCAAG GGAGAAGCAG GACTTGAGGG TCCACCAGGT 
AAAACTGGAC CAGTTGGTCC TCAAGGACCC TCTGGAAAGC CTGGTCCTGA AGGTCTCAGA 
GGAATCCCCG GTCCAGTCGG TGAACAAGGA CTTCCTGGTG CTCCTGGACA AGATGGTCCA 
CCTGGACCAA TGGGACCTCC TGGTCTGCCT GGCCTTAAAG GAGACCCTGG CTCTAAGGGT 
GAGAAGGGTC ACCCTGGTCT TATCGGTCTC ATCGGGCCGC CCGGTGAACA GGGAGAGAAG 
GGAGATCGGG GTTTGCCTGG ACCTCAGGGT GCTGCCGGCA GTAAGGGTGA TAATGGCATC 
AGTGGTGCTT CTGGTCCAAT TGGTCCTCCT GGTCCTCCTG GATTGCCTGG TCCACAAGGG 
CCAAAGGGAT CCAAGGGTTC AACTGGTCCT GCTGGTCAAA AAGGAGACAC TGGCACGGTT 
GGACCTCCTG GGCCTCCTGG TCCTCCTGGT GAGGTCATCC AGCCTCTGCC CATCCACAGT 
CCCAAGAAAT CCCGTCGCTC CATCGACATG CAGGCGGATG AAGCAGGCAC CATGCTGGAC 
TATGGAGAGG GCATGGAGGA CATATTCAGC TCTCTCAATA ATCTCAAACA GGACATCGAG 
CGCATGAAGT ACCCCATGGG CACGCAAAAC AACCCAGCAC GAAGCTGTAA AGACCTTCAG 
CTCGCTCACC CTGAATTCCC AGATGGCGAA TATTACATTG ATCCTAACCA GGGCTGCTCT 
GGAGACTCCT TCAAAGTGTA CTGCAACTTC ACAGCAGGAG GAGAGACCTG CATCTTCCCT 
GATAAAAAGT CAAATGGAGT GAGAATATCT TCATGGCCCA AAGAGGTGCC CGGCTCCTGG 
TTCAGTGAAT TTAAGCGTGG CAAAATACTT TCATACGTGG ACGCAGAAGG CAACTCGATA 
AACATGGTCC AGATGACGTT CCTCAAACTA CTGACAGCCT CCGCCAGGCA AAACCTGACC 
TACAGCTGCC ACCAGTCAGT GGCCTGGCAC GACGCCACTA CGGACAGCTA TGACAGAGCA 
TTACACTTCC TCGGCTCCAA CGACGAGGAG ATCTCATACG ACAACAACCC CTACATCAAG 
GCCCTGTCAG ACGGCTGTGC GGTGAGGAAG GGCTATGGAA AGACAGTACT GGAGATTAAC 
ACTCCCAAAA TTGATCAGGT TCCAATCGTC GACGTGAAGT TCACTGACTT TGGGGATCCC 
AACCAGAAAT TTGGATTTGA AGTTGGTCCA GTCTGCTTCC TTGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162875.1
CDS	282..5429
Translation	MERPRWSPMWKTKRWLLDSTVFKLITVSLVLVLQAPHVRAAEPVDVLKVLEFPTVPEGVEKTSGFCTNRRASQPDAAYKISKNAQLSAPTKQLFPDGVFPEDFSILTTIKPKAGIQSFLLSIYNEKGVQQLGVEVGRSPVFLYEDQEGKPAPEDYPLFRALNLADGKWHRVGISVEKKTVTIVVDCKKKLTKPLGRSDHASIDTKGITVFGTRILDEESFEGDIQQLLIVADPKAAYDYCEHYSPDCDVPHKDTLQAQEPGEEYTTPVEYGDLYDYYEGGTPTATDEFSEPQSNNVDGEYYTDSELDYGTVDASKTPVATTGPNKLGGGAALGQKGEKGEPAVIEPGMLIEGPSGPPGPAGLPGPPGLQGPPGLAGDPGDRGPPGRAGLPGADGLPGPPGTMLMLPFRFGGDGEKGPVVSAQEAQAQAILSQARLAMRGPPGPMGLTGRSGPVGLPGPPGLKGESGDHGPQGPRGIQGPPGQIGKPGKRGRAGADGARGMPGESGSKGDRGFDGLPGLPGEKGHRGETGPAGPPGPTGEDGPRGEDGEIGPRGLAGESGPRGLLGPRGPPGPPGQPGIAGVDGPQGPKGNMGPQGEPGPPGQQGIPGGQGLPGPQGPIGPPGEKGPQGRSGLPGLPGADGPPGHPGKEGPPGEKGALGQPGPQGPIGYPGPRGVKGADGVRGLKGGKGEKGEDGFPGFKGDMGLKGDRGELGLAGPRGEDGPEGPKGRAGPNGESGPQGSAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGGRGVAGKAGPRGQRGPTGPRGARGARGPTGKPGPKGTAGNDGPPGPPGERGPQGPQGPVGFPGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPGGVVGPQGPTGETGPIGERGHPGPPGPPGEQGLPGAGGKEGAKGDPGPQGSPGKDGPPGLRGFPGERGLPGVTGPPGLKGAEGPQGPPGPVGSPGERGAAGSAGPIGLPGRTGPQGPPGPAGEKGAPGERGPLGPAGRDGVQGPVGLPGPAGPQGPPGEDGDKGEVGEPGQKGSKADKGEQGPPGPPGLQGPIGAPGPAGADGEPGPRGQQGMFGQKGDEGSRGFPGPPGPIGLQGLPGPPGEKGENGDVGPMGPPGPPGPRGPQGPPGADGPQGPPGGIGGMGAVGEKGEQGEAGNPGPPGEPGPGGPKGERGEKGEAGPPGAAGPAGPKGPPGDDGPKGNPGPVGFPGDPGPPGEPGVAGIDGGPGEKGEDGESGQPGPPGPSGEAGPPGPPGKRGPPGAHGAEGRQGEKGAKGEAGLEGPPGKTGPVGPQGPSGKPGPEGLRGIPGPVGEQGLPGAPGQDGPPGPMGPPGLPGLKGDPGSKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGAAGSKGDNGISGASGPIGPPGPPGLPGPQGPKGSKGSTGPAGQKGDTGTVGPPGPPGPPGEVIQPLPIHSPKKSRRSIDMQADEAGTMLDYGEGMEDIFSSLNNLKQDIERMKYPMGTQNNPARSCKDLQLAHPEFPDGEYYIDPNQGCSGDSFKVYCNFTAGGETCIFPDKKSNGVRISSWPKEVPGSWFSEFKRGKILSYVDAEGNSINMVQMTFLKLLTASARQNLTYSCHQSVAWHDATTDSYDRALHFLGSNDEEISYDNNPYIKALSDGCAVRKGYGKTVLEINTPKIDQVPIVDVKFTDFGDPNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	XM_005162818.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type XI, alpha 1a (col11a1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162818.1

ORF Insert Sequence:

ATGGAGAGGC CAAGGTGGTC CCCAATGTGG AAAACGAAAA GGTGGCTTTT GGATTCCACT 
GTTTTCAAAT TGATCACAGT TTCCCTCGTC CTGGTTCTGC AGGCTCCACA TGTCAGAGCA 
GCAGAACCCG TGGATGTCTT AAAAGTATTA GAATTTCCCA CTGTCCCAGA AGGAGTAGAG 
AAAACGTCAG GATTCTGCAC AAACCGAAGA GCATCACAGC CAGACGCGGC CTACAAAATC 
AGCAAGAATG CCCAGCTCAG TGCCCCGACC AAGCAGCTTT TCCCAGATGG CGTTTTCCCT 
GAAGACTTCT CCATCCTAAC CACGATCAAG CCCAAAGCAG GGATCCAGTC CTTCCTCCTG 
TCCATCTACA ATGAGAAAGG CGTCCAGCAG CTGGGCGTGG AGGTGGGCCG TTCACCTGTC 
TTCCTGTACG AGGATCAGGA GGGCAAACCT GCCCCGGAGG ATTACCCTCT GTTCCGCGCT 
CTCAATCTGG CCGACGGAAA GTGGCATCGC GTAGGCATCA GCGTTGAGAA GAAGACAGTG 
ACTATTGTTG TCGACTGTAA GAAGAAGCTG ACCAAGCCAT TGGGCAGGAG TGACCATGCC 
AGTATCGACA CCAAGGGTAT CACCGTTTTT GGCACCAGGA TCCTGGATGA GGAATCTTTT 
GAGGGTGATA TACAACAACT GCTGATTGTG GCTGACCCTA AAGCAGCTTA CGACTACTGT 
GAACATTACA GCCCAGACTG TGACGTCCCT CACAAGGACA CACTGCAGGC ACAGGAGCCC 
GGAGAGGAGT ACACAACTCC TGTTGAATAT GGTGATTTAT ATGATTATTA CGAGGGAGGC 
ACACCCACAG CGACTGATGA GTTTTCTGAG CCGCAGTCTA ACAATGTAGA TGGAGAGTAC 
TATACAGACA GTGAACTTGA CTACGGAACA GTAGATGCTT CCAAAACGCC TGTCGCAACC 
ACTGGACCAA ACAAGCTGGG TGGAGGTGCT GCTCTTGGTC AGAAAGGTGA GAAAGGAGAG 
CCTGCAGTGA TTGAACCAGG AATGCTGATC GAGGGACCTT CAGGACCACC TGGACCTGCT 
GGTCTTCCAG GTCCTCCAGG TTTACAAGGC CCCCCTGGTT TGGCTGGAGA TCCTGGTGAT 
AGGGGTCCTC CAGGCCGTGC TGGTCTTCCT GGAGCTGATG GTTTGCCAGG ACCACCTGGA 
ACTATGCTCA TGCTGCCGTT TCGTTTTGGT GGTGATGGAG AAAAGGGCCC AGTGGTTTCA 
GCTCAGGAGG CTCAGGCTCA GGCTATTCTC TCTCAGGCCA GGCTCGCCAT GAGAGGTCCA 
CCGGGTCCAA TGGGTCTCAC TGGACGATCT GGACCAGTGG GTCTTCCAGG TCCTCCTGGA 
CTGAAGGGAG AAAGTGGGGA TCATGGTCCT CAGGGTCCAA GGGGAATCCA AGGCCCACCA 
GGGCAAATAG GAAAACCTGG AAAGAGGGGT CGTGCTGGAG CTGATGGTGC CCGAGGAATG 
CCTGGAGAGT CTGGATCTAA GGGTGACAGA GGTTTCGATG GGCTTCCGGG TCTGCCTGGT 
GAGAAAGGAC ACAGGGGTGA GACCGGCCCT GCTGGACCCC CGGGACCAAC TGGAGAGGAT 
GGACCAAGAG GAGAAGATGG TGAGATCGGA CCAAGAGGAC TTGCTGGTGA GAGCGGGCCG 
CGAGGTCTTC TCGGCCCAAG AGGCCCTCCT GGACCTCCTG GACAACCTGG CATTGCTGGT 
GTTGATGGTC CGCAGGGTCC CAAAGGAAAC ATGGGGCCTC AGGGAGAGCC AGGTCCTCCT 
GGACAGCAGG GAATCCCAGG AGGACAGGGT CTTCCAGGTC CACAGGGACC CATCGGACCA 
CCCGGAGAAA AAGGCCCTCA GGGAAGGTCT GGTTTGCCTG GATTGCCTGG TGCAGATGGG 
CCTCCTGGAC ATCCTGGTAA AGAGGGTCCA CCTGGAGAGA AAGGAGCTTT GGGCCAGCCT 
GGTCCCCAGG GCCCCATCGG CTATCCTGGC CCTCGCGGTG TGAAGGGTGC AGATGGTGTC 
CGTGGTCTAA AGGGAGGCAA AGGAGAGAAG GGTGAGGATG GATTCCCAGG TTTCAAGGGA 
GACATGGGTC TCAAAGGCGA CAGGGGAGAG CTGGGACTGG CTGGACCCAG AGGAGAAGAT 
GGTCCAGAGG GCCCGAAGGG TCGTGCAGGG CCTAACGGTG AATCTGGACC CCAGGGATCT 
GCCGGAGAAA AGGGCAAACT AGGTGTTCCA GGATTGCCCG GCTATCCAGG ACGTCAAGGG 
CCAAAGGGTT CGACCGGTTT CCCCGGATTC CCAGGAGCCA ATGGAGAGAA AGGAGGCAGG 
GGGGTGGCTG GGAAAGCTGG TCCAAGAGGC CAAAGAGGTC CAACGGGCCC CCGTGGTGCA 
CGAGGTGCAA GAGGCCCAAC AGGAAAGCCA GGCCCGAAGG GAACAGCAGG CAACGACGGT 
CCACCTGGCC CACCCGGTGA GAGAGGCCCT CAAGGACCCC AGGGTCCTGT TGGTTTCCCA 
GGACCAAAGG GTCCCCCTGG ACCACCTGGA AAGGATGGGC TGCCCGGTCA CCCTGGCCAG 
CGGGGAGAGA CTGGATTCCA AGGCAAAACT GGCCCACCAG GACCTGGAGG TGTTGTTGGA 
CCACAAGGAC CGACTGGAGA AACCGGTCCA ATTGGTGAAA GAGGACATCC TGGACCCCCT 
GGTCCCCCCG GAGAGCAGGG TCTTCCTGGC GCTGGTGGAA AAGAAGGGGC AAAGGGAGAT 
CCAGGTCCTC AGGGCAGTCC AGGAAAAGAC GGTCCACCAG GACTCAGAGG GTTCCCTGGA 
GAAAGAGGTC TTCCAGGAGT GACGGGTCCA CCTGGTTTGA AAGGCGCCGA GGGCCCTCAG 
GGCCCTCCTG GCCCTGTTGG TTCCCCTGGT GAGAGAGGAG CAGCAGGATC TGCTGGTCCC 
ATTGGCCTGC CTGGACGTAC TGGACCTCAG GGACCCCCAG GACCTGCTGG AGAGAAGGGT 
GCACCGGGAG AGAGAGGTCC TCTGGGTCCA GCCGGTAGGG ATGGAGTTCA AGGTCCAGTT 
GGTCTGCCAG GTCCAGCAGG ACCTCAGGGT CCACCTGGAG AAGATGGTGA CAAGGGAGAA 
GTGGGTGAAC CAGGCCAGAA GGGAAGCAAA GCTGACAAAG GAGAACAGGG TCCACCAGGT 
CCTCCTGGTC TTCAGGGACC CATTGGAGCT CCTGGACCTG CTGGAGCAGA TGGCGAGCCC 
GGTCCTCGTG GACAGCAGGG TATGTTTGGA CAGAAGGGAG ATGAAGGATC CCGGGGCTTC 
CCTGGACCTC CTGGTCCCAT CGGTCTGCAG GGTCTTCCAG GACCACCTGG CGAGAAAGGT 
GAAAATGGAG ATGTTGGTCC TATGGGTCCT CCTGGTCCAC CAGGTCCCAG AGGACCTCAG 
GGTCCTCCTG GTGCTGATGG CCCGCAAGGT CCTCCTGGTG GAATAGGAGG AATGGGAGCC 
GTGGGTGAGA AGGGTGAGCA AGGAGAAGCT GGCAACCCTG GACCTCCTGG AGAGCCTGGA 
CCTGGGGGCC CGAAAGGTGA GAGAGGAGAG AAAGGTGAAG CTGGTCCCCC TGGTGCTGCT 
GGACCTGCAG GTCCCAAAGG ACCCCCTGGA GATGATGGAC CCAAGGGTAA CCCTGGCCCT 
GTTGGTTTTC CTGGAGACCC AGGTCCACCT GGTGAGCCAG GTGTTGCTGG AATAGATGGA 
GGCCCAGGAG AGAAGGGAGA GGATGGAGAG TCTGGTCAGC CTGGACCCCC TGGTCCTTCT 
GGAGAAGCTG GTCCACCAGG ACCTCCTGGA AAACGAGGAC CCCCGGGGGC GCATGGAGCT 
GAAGGCAGAC AAGGAGAGAA GGGCGCCAAG GGAGAAGCAG GACTTGAGGG TCCACCAGGT 
AAAACTGGAC CAGTTGGTCC TCAAGGACCC TCTGGAAAGC CTGGTCCTGA AGGTCTCAGA 
GGAATCCCCG GTCCAGTCGG TGAACAAGGA CTTCCTGGTG CTCCTGGACA AGATGGTCCA 
CCTGGACCAA TGGGACCTCC TGGTCTGCCT GGCCTTAAAG GAGACCCTGG CTCTAAGGGT 
GAGAAGGGTC ACCCTGGTCT TATCGGTCTC ATCGGGCCGC CCGGTGAACA GGGAGAGAAG 
GGAGATCGGG GTTTGCCTGG ACCTCAGGGT GCTGCCGGCA GTAAGGGTGA TAATGGCATC 
AGTGGTGCTT CTGGTCCAAT TGGTCCTCCT GGTCCTCCTG GATTGCCTGG TCCACAAGGG 
CCAAAGGGAT CCAAGGGTTC AACTGGTCCT GCTGGTCAAA AAGGAGACAC TGGCACGGTT 
GGACCTCCTG GGCCTCCTGG TCCTCCTGGT GAGGTCATCC AGCCTCTGCC CATCCACAGT 
CCCAAGAAAT CCCGTCGCTC CATCGACATG CAGGCGGATG AAGCAGGCAC CATGCTGGAC 
TATGGAGAGG GCATGGAGGA CATATTCAGC TCTCTCAATA ATCTCAAACA GGACATCGAG 
CGCATGAAGT ACCCCATGGG CACGCAAAAC AACCCAGCAC GAAGCTGTAA AGACCTTCAG 
CTCGCTCACC CTGAATTCCC AGATGGCGAA TATTACATTG ATCCTAACCA GGGCTGCTCT 
GGAGACTCCT TCAAAGTGTA CTGCAACTTC ACAGCAGGAG GAGAGACCTG CATCTTCCCT 
GATAAAAAGT CAAATGGAGT GAGAATATCT TCATGGCCCA AAGAGGTGCC CGGCTCCTGG 
TTCAGTGAAT TTAAGCGTGG CAAAATACTT TCATACGTGG ACGCAGAAGG CAACTCGATA 
AACATGGTCC AGATGACGTT CCTCAAACTA CTGACAGCCT CCGCCAGGCA AAACCTGACC 
TACAGCTGCC ACCAGTCAGT GGCCTGGCAC GACGCCACTA CGGACAGCTA TGACAGAGCA 
TTACACTTCC TCGGCTCCAA CGACGAGGAG ATCTCATACG ACAACAACCC CTACATCAAG 
GCCCTGTCAG ACGGCTGTGC GGTGAGGAAG GGCTATGGAA AGACAGTACT GGAGATTAAC 
ACTCCCAAAA TTGATCAGGT TCCAATCGTC GACGTGAAGT TCACTGACTT TGGGGATCCC 
AACCAGAAAT TTGGATTTGA AGTTGGTCCA GTCTGCTTCC TTGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col11a1a ( XM_017353843.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209332.1 Danio rerio collagen, type XI, alpha 1a (col11a1a), transcript variant X4, mRNA.

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CloneID	ODa57601
Clone ID Related Accession (Same CDS sequence)	XM_017353843.1
Accession Version	XM_017353843.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-1(XI) chain isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGGC CAAGGTGGTC CCCAATGTGG AAAACGAAAA GGTGGCTTTT GGATTCCACT 
GTTTTCAAAT TGATCACAGT TTCCCTCGTC CTGGTTCTGC AGGCTCCACA TGTCAGAGCA 
GCAGAACCCG TGGATGTCTT AAAAGTATTA GAATTTCCCA CTGTCCCAGA AGGAGTAGAG 
AAAACGTCAG GATTCTGCAC AAACCGAAGA GCATCACAGC CAGACGCGGC CTACAAAATC 
AGCAAGAATG CCCAGCTCAG TGCCCCGACC AAGCAGCTTT TCCCAGATGG CGTTTTCCCT 
GAAGACTTCT CCATCCTAAC CACGATCAAG CCCAAAGCAG GGATCCAGTC CTTCCTCCTG 
TCCATCTACA ATGAGAAAGG CGTCCAGCAG CTGGGCGTGG AGGTGGGCCG TTCACCTGTC 
TTCCTGTACG AGGATCAGGA GGGCAAACCT GCCCCGGAGG ATTACCCTCT GTTCCGCGCT 
CTCAATCTGG CCGACGGAAA GTGGCATCGC GTAGGCATCA GCGTTGAGAA GAAGACAGTG 
ACTATTGTTG TCGACTGTAA GAAGAAGCTG ACCAAGCCAT TGGGCAGGAG TGACCATGCC 
AGTATCGACA CCAAGGGTAT CACCGTTTTT GGCACCAGGA TCCTGGATGA GGAATCTTTT 
GAGGGTGATA TACAACAACT GCTGATTGTG GCTGACCCTA AAGCAGCTTA CGACTACTGT 
GAACATTACA GCCCAGACTG TGACGTCCCT CACAAGGACA CACTGCAGGC ACAGGAGCCC 
GGAGAGGAGT ACACAACTCC TGTTGAATAT GGTGATTTAT ATGATTATTA CGAGGGAGGC 
ACACCCACAG CGACTGATGA GTTTTCTGAG CCGCAGAGAG TGAAACGTAG TGTCTCCATG 
AAATCCAAGA GGACAAAAAA GTCCCCTAAA TCTTTTGCCA AACCCTATAA GTACCTGGAG 
CCAAAACAGG CTAAAAAGTT AGTGCCTAAG AAGAAGAGAT ACGGATACCT GCCACCAACA 
CCAAGACAAG CCATTCTAGG GCTGGGTGGA GGTGCTGCTC TTGGTCAGAA AGGTGAGAAA 
GGAGAGCCTG CAGTGATTGA ACCAGGAATG CTGATCGAGG GACCTTCAGG ACCACCTGGA 
CCTGCTGGTC TTCCAGGTCC TCCAGGTTTA CAAGGCCCCC CTGGTTTGGC TGGAGATCCT 
GGTGATAGGG GTCCTCCAGG CCGTGCTGGT CTTCCTGGAG CTGATGGTTT GCCAGGACCA 
CCTGGAACTA TGCTCATGCT GCCGTTTCGT TTTGGTGGTG ATGGAGAAAA GGGCCCAGTG 
GTTTCAGCTC AGGAGGCTCA GGCTCAGGCT ATTCTCTCTC AGGCCAGGCT CGCCATGAGA 
GGTCCACCGG GTCCAATGGG TCTCACTGGA CGATCTGGAC CAGTGGGTCT TCCAGGTCCT 
CCTGGACTGA AGGGAGAAAG TGGGGATCAT GGTCCTCAGG GTCCAAGGGG AATCCAAGGC 
CCACCAGGGC AAATAGGAAA ACCTGGAAAG AGGGGTCGTG CTGGAGCTGA TGGTGCCCGA 
GGAATGCCTG GAGAGTCTGG ATCTAAGGGT GACAGAGGTT TCGATGGGCT TCCGGGTCTG 
CCTGGTGAGA AAGGACACAG GGGTGAGACC GGCCCTGCTG GACCCCCGGG ACCAACTGGA 
GAGGATGGAC CAAGAGGAGA AGATGGTGAG ATCGGACCAA GAGGACTTGC TGGTGAGAGC 
GGGCCGCGAG GTCTTCTCGG CCCAAGAGGC CCTCCTGGAC CTCCTGGACA ACCTGGCATT 
GCTGGTGTTG ATGGTCCGCA GGGTCCCAAA GGAAACATGG GGCCTCAGGG AGAGCCAGGT 
CCTCCTGGAC AGCAGGGAAT CCCAGGAGGA CAGGGTCTTC CAGGTCCACA GGGACCCATC 
GGACCACCCG GAGAAAAAGG CCCTCAGGGA AGGTCTGGTT TGCCTGGATT GCCTGGTGCA 
GATGGGCCTC CTGGACATCC TGGTAAAGAG GGTCCACCTG GAGAGAAAGG AGCTTTGGGC 
CAGCCTGGTC CCCAGGGCCC CATCGGCTAT CCTGGCCCTC GCGGTGTGAA GGGTGCAGAT 
GGTGTCCGTG GTCTAAAGGG AGGCAAAGGA GAGAAGGGTG AGGATGGATT CCCAGGTTTC 
AAGGGAGACA TGGGTCTCAA AGGCGACAGG GGAGAGCTGG GACTGGCTGG ACCCAGAGGA 
GAAGATGGTC CAGAGGGCCC GAAGGGTCGT GCAGGGCCTA ACGGTGAATC TGGACCCCAG 
GGATCTGCCG GAGAAAAGGG CAAACTAGGT GTTCCAGGAT TGCCCGGCTA TCCAGGACGT 
CAAGGGCCAA AGGGTTCGAC CGGTTTCCCC GGATTCCCAG GAGCCAATGG AGAGAAAGGA 
GGCAGGGGGG TGGCTGGGAA AGCTGGTCCA AGAGGCCAAA GAGGTCCAAC GGGCCCCCGT 
GGTGCACGAG GTGCAAGAGG CCCAACAGGA AAGCCAGGCC CGAAGGGAAC AGCAGGCAAC 
GACGGTCCAC CTGGCCCACC CGGTGAGAGA GGCCCTCAAG GACCCCAGGG TCCTGTTGGT 
TTCCCAGGAC CAAAGGGTCC CCCTGGACCA CCTGGAAAGG ATGGGCTGCC CGGTCACCCT 
GGCCAGCGGG GAGAGACTGG ATTCCAAGGC AAAACTGGCC CACCAGGACC TGGAGGTGTT 
GTTGGACCAC AAGGACCGAC TGGAGAAACC GGTCCAATTG GTGAAAGAGG ACATCCTGGA 
CCCCCTGGTC CCCCCGGAGA GCAGGGTCTT CCTGGCGCTG GTGGAAAAGA AGGGGCAAAG 
GGAGATCCAG GTCCTCAGGG CAGTCCAGGA AAAGACGGTC CACCAGGACT CAGAGGGTTC 
CCTGGAGAAA GAGGTCTTCC AGGAGTGACG GGTCCACCTG GTTTGAAAGG CGCCGAGGGC 
CCTCAGGGCC CTCCTGGCCC TGTTGGTTCC CCTGGTGAGA GAGGAGCAGC AGGATCTGCT 
GGTCCCATTG GCCTGCCTGG ACGTACTGGA CCTCAGGGAC CCCCAGGACC TGCTGGAGAG 
AAGGGTGCAC CGGGAGAGAG AGGTCCTCTG GGTCCAGCCG GTAGGGATGG AGTTCAAGGT 
CCAGTTGGTC TGCCAGGTCC AGCAGGACCT CAGGGTCCAC CTGGAGAAGA TGGTGACAAG 
GGAGAAGTGG GTGAACCAGG CCAGAAGGGA AGCAAAGCTG ACAAAGGAGA ACAGGGTCCA 
CCAGGTCCTC CTGGTCTTCA GGGACCCATT GGAGCTCCTG GACCTGCTGG AGCAGATGGC 
GAGCCCGGTC CTCGTGGACA GCAGGGTATG TTTGGACAGA AGGGAGATGA AGGATCCCGG 
GGCTTCCCTG GACCTCCTGG TCCCATCGGT CTGCAGGGTC TTCCAGGACC ACCTGGCGAG 
AAAGGTGAAA ATGGAGATGT TGGTCCTATG GGTCCTCCTG GTCCACCAGG TCCCAGAGGA 
CCTCAGGGTC CTCCTGGTGC TGATGGCCCG CAAGGTCCTC CTGGTGGAAT AGGAGGAATG 
GGAGCCGTGG GTGAGAAGGG TGAGCAAGGA GAAGCTGGCA ACCCTGGACC TCCTGGAGAG 
CCTGGACCTG GGGGCCCGAA AGGTGAGAGA GGAGAGAAAG GTGAAGCTGG TCCCCCTGGT 
GCTGCTGGAC CTGCAGGTCC CAAAGGACCC CCTGGAGATG ATGGACCCAA GGGTAACCCT 
GGCCCTGTTG GTTTTCCTGG AGACCCAGGT CCACCTGGTG AGCCAGGTGT TGCTGGAATA 
GATGGAGGCC CAGGAGAGAA GGGAGAGGAT GGAGAGTCTG GTCAGCCTGG ACCCCCTGGT 
CCTTCTGGAG AAGCTGGTCC ACCAGGACCT CCTGGAAAAC GAGGACCCCC GGGGGCGCAT 
GGAGCTGAAG GCAGACAAGG AGAGAAGGGC GCCAAGGGAG AAGCAGGACT TGAGGGTCCA 
CCAGGTAAAA CTGGACCAGT TGGTCCTCAA GGACCCTCTG GAAAGCCTGG TCCTGAAGGT 
CTCAGAGGAA TCCCCGGTCC AGTCGGTGAA CAAGGACTTC CTGGTGCTCC TGGACAAGAT 
GGTCCACCTG GACCAATGGG ACCTCCTGGT CTGCCTGGCC TTAAAGGAGA CCCTGGCTCT 
AAGGGTGAGA AGGGTCACCC TGGTCTTATC GGTCTCATCG GGCCGCCCGG TGAACAGGGA 
GAGAAGGGAG ATCGGGGTTT GCCTGGACCT CAGGGTGCTG CCGGCAGTAA GGGTGATAAT 
GGCATCAGTG GTGCTTCTGG TCCAATTGGT CCTCCTGGTC CTCCTGGATT GCCTGGTCCA 
CAAGGGCCAA AGGGATCCAA GGGTTCAACT GGTCCTGCTG GTCAAAAAGG AGACACTGGC 
ACGGTTGGAC CTCCTGGGCC TCCTGGTCCT CCTGGTGAGG TCATCCAGCC TCTGCCCATC 
CACAGTCCCA AGAAATCCCG TCGCTCCATC GACATGCAGG CGGATGAAGC AGGCACCATG 
CTGGACTATG GAGAGGGCAT GGAGGACATA TTCAGCTCTC TCAATAATCT CAAACAGGAC 
ATCGAGCGCA TGAAGTACCC CATGGGCACG CAAAACAACC CAGCACGAAG CTGTAAAGAC 
CTTCAGCTCG CTCACCCTGA ATTCCCAGAT GGCGAATATT ACATTGATCC TAACCAGGGC 
TGCTCTGGAG ACTCCTTCAA AGTGTACTGC AACTTCACAG CAGGAGGAGA GACCTGCATC 
TTCCCTGATA AAAAGTCAAA TGGAGTGAGA ATATCTTCAT GGCCCAAAGA GGTGCCCGGC 
TCCTGGTTCA GTGAATTTAA GCGTGGCAAA ATACTTTCAT ACGTGGACGC AGAAGGCAAC 
TCGATAAACA TGGTCCAGAT GACGTTCCTC AAACTACTGA CAGCCTCCGC CAGGCAAAAC 
CTGACCTACA GCTGCCACCA GTCAGTGGCC TGGCACGACG CCACTACGGA CAGCTATGAC 
AGAGCATTAC ACTTCCTCGG CTCCAACGAC GAGGAGATCT CATACGACAA CAACCCCTAC 
ATCAAGGCCC TGTCAGACGG CTGTGCGGTG AGGAAGGGCT ATGGAAAGAC AGTACTGGAG 
ATTAACACTC CCAAAATTGA TCAGGTTCCA ATCGTCGACG TGAAGTTCAC TGACTTTGGG 
GATCCCAACC AGAAATTTGG ATTTGAAGTT GGTCCAGTCT GCTTCCTTGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209332.1
CDS	282..5495
Translation	MERPRWSPMWKTKRWLLDSTVFKLITVSLVLVLQAPHVRAAEPVDVLKVLEFPTVPEGVEKTSGFCTNRRASQPDAAYKISKNAQLSAPTKQLFPDGVFPEDFSILTTIKPKAGIQSFLLSIYNEKGVQQLGVEVGRSPVFLYEDQEGKPAPEDYPLFRALNLADGKWHRVGISVEKKTVTIVVDCKKKLTKPLGRSDHASIDTKGITVFGTRILDEESFEGDIQQLLIVADPKAAYDYCEHYSPDCDVPHKDTLQAQEPGEEYTTPVEYGDLYDYYEGGTPTATDEFSEPQRVKRSVSMKSKRTKKSPKSFAKPYKYLEPKQAKKLVPKKKRYGYLPPTPRQAILGLGGGAALGQKGEKGEPAVIEPGMLIEGPSGPPGPAGLPGPPGLQGPPGLAGDPGDRGPPGRAGLPGADGLPGPPGTMLMLPFRFGGDGEKGPVVSAQEAQAQAILSQARLAMRGPPGPMGLTGRSGPVGLPGPPGLKGESGDHGPQGPRGIQGPPGQIGKPGKRGRAGADGARGMPGESGSKGDRGFDGLPGLPGEKGHRGETGPAGPPGPTGEDGPRGEDGEIGPRGLAGESGPRGLLGPRGPPGPPGQPGIAGVDGPQGPKGNMGPQGEPGPPGQQGIPGGQGLPGPQGPIGPPGEKGPQGRSGLPGLPGADGPPGHPGKEGPPGEKGALGQPGPQGPIGYPGPRGVKGADGVRGLKGGKGEKGEDGFPGFKGDMGLKGDRGELGLAGPRGEDGPEGPKGRAGPNGESGPQGSAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGGRGVAGKAGPRGQRGPTGPRGARGARGPTGKPGPKGTAGNDGPPGPPGERGPQGPQGPVGFPGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPGGVVGPQGPTGETGPIGERGHPGPPGPPGEQGLPGAGGKEGAKGDPGPQGSPGKDGPPGLRGFPGERGLPGVTGPPGLKGAEGPQGPPGPVGSPGERGAAGSAGPIGLPGRTGPQGPPGPAGEKGAPGERGPLGPAGRDGVQGPVGLPGPAGPQGPPGEDGDKGEVGEPGQKGSKADKGEQGPPGPPGLQGPIGAPGPAGADGEPGPRGQQGMFGQKGDEGSRGFPGPPGPIGLQGLPGPPGEKGENGDVGPMGPPGPPGPRGPQGPPGADGPQGPPGGIGGMGAVGEKGEQGEAGNPGPPGEPGPGGPKGERGEKGEAGPPGAAGPAGPKGPPGDDGPKGNPGPVGFPGDPGPPGEPGVAGIDGGPGEKGEDGESGQPGPPGPSGEAGPPGPPGKRGPPGAHGAEGRQGEKGAKGEAGLEGPPGKTGPVGPQGPSGKPGPEGLRGIPGPVGEQGLPGAPGQDGPPGPMGPPGLPGLKGDPGSKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGAAGSKGDNGISGASGPIGPPGPPGLPGPQGPKGSKGSTGPAGQKGDTGTVGPPGPPGPPGEVIQPLPIHSPKKSRRSIDMQADEAGTMLDYGEGMEDIFSSLNNLKQDIERMKYPMGTQNNPARSCKDLQLAHPEFPDGEYYIDPNQGCSGDSFKVYCNFTAGGETCIFPDKKSNGVRISSWPKEVPGSWFSEFKRGKILSYVDAEGNSINMVQMTFLKLLTASARQNLTYSCHQSVAWHDATTDSYDRALHFLGSNDEEISYDNNPYIKALSDGCAVRKGYGKTVLEINTPKIDQVPIVDVKFTDFGDPNQKFGFEVGPVCFLG

Target ORF information:

RefSeq Version	XM_017353843.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type XI, alpha 1a (col11a1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353843.1

ORF Insert Sequence:

ATGGAGAGGC CAAGGTGGTC CCCAATGTGG AAAACGAAAA GGTGGCTTTT GGATTCCACT 
GTTTTCAAAT TGATCACAGT TTCCCTCGTC CTGGTTCTGC AGGCTCCACA TGTCAGAGCA 
GCAGAACCCG TGGATGTCTT AAAAGTATTA GAATTTCCCA CTGTCCCAGA AGGAGTAGAG 
AAAACGTCAG GATTCTGCAC AAACCGAAGA GCATCACAGC CAGACGCGGC CTACAAAATC 
AGCAAGAATG CCCAGCTCAG TGCCCCGACC AAGCAGCTTT TCCCAGATGG CGTTTTCCCT 
GAAGACTTCT CCATCCTAAC CACGATCAAG CCCAAAGCAG GGATCCAGTC CTTCCTCCTG 
TCCATCTACA ATGAGAAAGG CGTCCAGCAG CTGGGCGTGG AGGTGGGCCG TTCACCTGTC 
TTCCTGTACG AGGATCAGGA GGGCAAACCT GCCCCGGAGG ATTACCCTCT GTTCCGCGCT 
CTCAATCTGG CCGACGGAAA GTGGCATCGC GTAGGCATCA GCGTTGAGAA GAAGACAGTG 
ACTATTGTTG TCGACTGTAA GAAGAAGCTG ACCAAGCCAT TGGGCAGGAG TGACCATGCC 
AGTATCGACA CCAAGGGTAT CACCGTTTTT GGCACCAGGA TCCTGGATGA GGAATCTTTT 
GAGGGTGATA TACAACAACT GCTGATTGTG GCTGACCCTA AAGCAGCTTA CGACTACTGT 
GAACATTACA GCCCAGACTG TGACGTCCCT CACAAGGACA CACTGCAGGC ACAGGAGCCC 
GGAGAGGAGT ACACAACTCC TGTTGAATAT GGTGATTTAT ATGATTATTA CGAGGGAGGC 
ACACCCACAG CGACTGATGA GTTTTCTGAG CCGCAGAGAG TGAAACGTAG TGTCTCCATG 
AAATCCAAGA GGACAAAAAA GTCCCCTAAA TCTTTTGCCA AACCCTATAA GTACCTGGAG 
CCAAAACAGG CTAAAAAGTT AGTGCCTAAG AAGAAGAGAT ACGGATACCT GCCACCAACA 
CCAAGACAAG CCATTCTAGG GCTGGGTGGA GGTGCTGCTC TTGGTCAGAA AGGTGAGAAA 
GGAGAGCCTG CAGTGATTGA ACCAGGAATG CTGATCGAGG GACCTTCAGG ACCACCTGGA 
CCTGCTGGTC TTCCAGGTCC TCCAGGTTTA CAAGGCCCCC CTGGTTTGGC TGGAGATCCT 
GGTGATAGGG GTCCTCCAGG CCGTGCTGGT CTTCCTGGAG CTGATGGTTT GCCAGGACCA 
CCTGGAACTA TGCTCATGCT GCCGTTTCGT TTTGGTGGTG ATGGAGAAAA GGGCCCAGTG 
GTTTCAGCTC AGGAGGCTCA GGCTCAGGCT ATTCTCTCTC AGGCCAGGCT CGCCATGAGA 
GGTCCACCGG GTCCAATGGG TCTCACTGGA CGATCTGGAC CAGTGGGTCT TCCAGGTCCT 
CCTGGACTGA AGGGAGAAAG TGGGGATCAT GGTCCTCAGG GTCCAAGGGG AATCCAAGGC 
CCACCAGGGC AAATAGGAAA ACCTGGAAAG AGGGGTCGTG CTGGAGCTGA TGGTGCCCGA 
GGAATGCCTG GAGAGTCTGG ATCTAAGGGT GACAGAGGTT TCGATGGGCT TCCGGGTCTG 
CCTGGTGAGA AAGGACACAG GGGTGAGACC GGCCCTGCTG GACCCCCGGG ACCAACTGGA 
GAGGATGGAC CAAGAGGAGA AGATGGTGAG ATCGGACCAA GAGGACTTGC TGGTGAGAGC 
GGGCCGCGAG GTCTTCTCGG CCCAAGAGGC CCTCCTGGAC CTCCTGGACA ACCTGGCATT 
GCTGGTGTTG ATGGTCCGCA GGGTCCCAAA GGAAACATGG GGCCTCAGGG AGAGCCAGGT 
CCTCCTGGAC AGCAGGGAAT CCCAGGAGGA CAGGGTCTTC CAGGTCCACA GGGACCCATC 
GGACCACCCG GAGAAAAAGG CCCTCAGGGA AGGTCTGGTT TGCCTGGATT GCCTGGTGCA 
GATGGGCCTC CTGGACATCC TGGTAAAGAG GGTCCACCTG GAGAGAAAGG AGCTTTGGGC 
CAGCCTGGTC CCCAGGGCCC CATCGGCTAT CCTGGCCCTC GCGGTGTGAA GGGTGCAGAT 
GGTGTCCGTG GTCTAAAGGG AGGCAAAGGA GAGAAGGGTG AGGATGGATT CCCAGGTTTC 
AAGGGAGACA TGGGTCTCAA AGGCGACAGG GGAGAGCTGG GACTGGCTGG ACCCAGAGGA 
GAAGATGGTC CAGAGGGCCC GAAGGGTCGT GCAGGGCCTA ACGGTGAATC TGGACCCCAG 
GGATCTGCCG GAGAAAAGGG CAAACTAGGT GTTCCAGGAT TGCCCGGCTA TCCAGGACGT 
CAAGGGCCAA AGGGTTCGAC CGGTTTCCCC GGATTCCCAG GAGCCAATGG AGAGAAAGGA 
GGCAGGGGGG TGGCTGGGAA AGCTGGTCCA AGAGGCCAAA GAGGTCCAAC GGGCCCCCGT 
GGTGCACGAG GTGCAAGAGG CCCAACAGGA AAGCCAGGCC CGAAGGGAAC AGCAGGCAAC 
GACGGTCCAC CTGGCCCACC CGGTGAGAGA GGCCCTCAAG GACCCCAGGG TCCTGTTGGT 
TTCCCAGGAC CAAAGGGTCC CCCTGGACCA CCTGGAAAGG ATGGGCTGCC CGGTCACCCT 
GGCCAGCGGG GAGAGACTGG ATTCCAAGGC AAAACTGGCC CACCAGGACC TGGAGGTGTT 
GTTGGACCAC AAGGACCGAC TGGAGAAACC GGTCCAATTG GTGAAAGAGG ACATCCTGGA 
CCCCCTGGTC CCCCCGGAGA GCAGGGTCTT CCTGGCGCTG GTGGAAAAGA AGGGGCAAAG 
GGAGATCCAG GTCCTCAGGG CAGTCCAGGA AAAGACGGTC CACCAGGACT CAGAGGGTTC 
CCTGGAGAAA GAGGTCTTCC AGGAGTGACG GGTCCACCTG GTTTGAAAGG CGCCGAGGGC 
CCTCAGGGCC CTCCTGGCCC TGTTGGTTCC CCTGGTGAGA GAGGAGCAGC AGGATCTGCT 
GGTCCCATTG GCCTGCCTGG ACGTACTGGA CCTCAGGGAC CCCCAGGACC TGCTGGAGAG 
AAGGGTGCAC CGGGAGAGAG AGGTCCTCTG GGTCCAGCCG GTAGGGATGG AGTTCAAGGT 
CCAGTTGGTC TGCCAGGTCC AGCAGGACCT CAGGGTCCAC CTGGAGAAGA TGGTGACAAG 
GGAGAAGTGG GTGAACCAGG CCAGAAGGGA AGCAAAGCTG ACAAAGGAGA ACAGGGTCCA 
CCAGGTCCTC CTGGTCTTCA GGGACCCATT GGAGCTCCTG GACCTGCTGG AGCAGATGGC 
GAGCCCGGTC CTCGTGGACA GCAGGGTATG TTTGGACAGA AGGGAGATGA AGGATCCCGG 
GGCTTCCCTG GACCTCCTGG TCCCATCGGT CTGCAGGGTC TTCCAGGACC ACCTGGCGAG 
AAAGGTGAAA ATGGAGATGT TGGTCCTATG GGTCCTCCTG GTCCACCAGG TCCCAGAGGA 
CCTCAGGGTC CTCCTGGTGC TGATGGCCCG CAAGGTCCTC CTGGTGGAAT AGGAGGAATG 
GGAGCCGTGG GTGAGAAGGG TGAGCAAGGA GAAGCTGGCA ACCCTGGACC TCCTGGAGAG 
CCTGGACCTG GGGGCCCGAA AGGTGAGAGA GGAGAGAAAG GTGAAGCTGG TCCCCCTGGT 
GCTGCTGGAC CTGCAGGTCC CAAAGGACCC CCTGGAGATG ATGGACCCAA GGGTAACCCT 
GGCCCTGTTG GTTTTCCTGG AGACCCAGGT CCACCTGGTG AGCCAGGTGT TGCTGGAATA 
GATGGAGGCC CAGGAGAGAA GGGAGAGGAT GGAGAGTCTG GTCAGCCTGG ACCCCCTGGT 
CCTTCTGGAG AAGCTGGTCC ACCAGGACCT CCTGGAAAAC GAGGACCCCC GGGGGCGCAT 
GGAGCTGAAG GCAGACAAGG AGAGAAGGGC GCCAAGGGAG AAGCAGGACT TGAGGGTCCA 
CCAGGTAAAA CTGGACCAGT TGGTCCTCAA GGACCCTCTG GAAAGCCTGG TCCTGAAGGT 
CTCAGAGGAA TCCCCGGTCC AGTCGGTGAA CAAGGACTTC CTGGTGCTCC TGGACAAGAT 
GGTCCACCTG GACCAATGGG ACCTCCTGGT CTGCCTGGCC TTAAAGGAGA CCCTGGCTCT 
AAGGGTGAGA AGGGTCACCC TGGTCTTATC GGTCTCATCG GGCCGCCCGG TGAACAGGGA 
GAGAAGGGAG ATCGGGGTTT GCCTGGACCT CAGGGTGCTG CCGGCAGTAA GGGTGATAAT 
GGCATCAGTG GTGCTTCTGG TCCAATTGGT CCTCCTGGTC CTCCTGGATT GCCTGGTCCA 
CAAGGGCCAA AGGGATCCAA GGGTTCAACT GGTCCTGCTG GTCAAAAAGG AGACACTGGC 
ACGGTTGGAC CTCCTGGGCC TCCTGGTCCT CCTGGTGAGG TCATCCAGCC TCTGCCCATC 
CACAGTCCCA AGAAATCCCG TCGCTCCATC GACATGCAGG CGGATGAAGC AGGCACCATG 
CTGGACTATG GAGAGGGCAT GGAGGACATA TTCAGCTCTC TCAATAATCT CAAACAGGAC 
ATCGAGCGCA TGAAGTACCC CATGGGCACG CAAAACAACC CAGCACGAAG CTGTAAAGAC 
CTTCAGCTCG CTCACCCTGA ATTCCCAGAT GGCGAATATT ACATTGATCC TAACCAGGGC 
TGCTCTGGAG ACTCCTTCAA AGTGTACTGC AACTTCACAG CAGGAGGAGA GACCTGCATC 
TTCCCTGATA AAAAGTCAAA TGGAGTGAGA ATATCTTCAT GGCCCAAAGA GGTGCCCGGC 
TCCTGGTTCA GTGAATTTAA GCGTGGCAAA ATACTTTCAT ACGTGGACGC AGAAGGCAAC 
TCGATAAACA TGGTCCAGAT GACGTTCCTC AAACTACTGA CAGCCTCCGC CAGGCAAAAC 
CTGACCTACA GCTGCCACCA GTCAGTGGCC TGGCACGACG CCACTACGGA CAGCTATGAC 
AGAGCATTAC ACTTCCTCGG CTCCAACGAC GAGGAGATCT CATACGACAA CAACCCCTAC 
ATCAAGGCCC TGTCAGACGG CTGTGCGGTG AGGAAGGGCT ATGGAAAGAC AGTACTGGAG 
ATTAACACTC CCAAAATTGA TCAGGTTCCA ATCGTCGACG TGAAGTTCAC TGACTTTGGG 
GATCCCAACC AGAAATTTGG ATTTGAAGTT GGTCCAGTCT GCTTCCTTGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular Mechanisms of Developmental Toxicity Induced by Graphene Oxide at Predicted Environmental Concentrations.
Environmental science & technology51(14)7861-7871(2017 Jul)
Zhang X,Zhou Q,Zou W,Hu X

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col11a1a cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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