cacna1ha cDNA ORF clone, Danio rerio(zebrafish)

The following cacna1ha gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cacna1ha cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa141818 XM_021474706.1
Latest version!
Danio rerio calcium channel, voltage-dependent, T type, alpha 1H subunit a (cacna1ha), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa141819 XM_021474707.1
Latest version!
Danio rerio calcium channel, voltage-dependent, T type, alpha 1H subunit a (cacna1ha), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa43366 XM_009299684.3
Latest version!
Danio rerio calcium channel, voltage-dependent, T type, alpha 1H subunit a (cacna1ha), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa43367 XM_009299685.3
Latest version!
Danio rerio calcium channel, voltage-dependent, T type, alpha 1H subunit a (cacna1ha), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa43368 XM_009299686.3
Latest version!
Danio rerio calcium channel, voltage-dependent, T type, alpha 1H subunit a (cacna1ha), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
ODa43366 XM_009299684.2 Danio rerio calcium channel, voltage-dependent, T type, alpha 1H subunit a (cacna1ha), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa43367 XM_009299685.2 Danio rerio calcium channel, voltage-dependent, T type, alpha 1H subunit a (cacna1ha), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa43368 XM_009299686.2 Danio rerio calcium channel, voltage-dependent, T type, alpha 1H subunit a (cacna1ha), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa141818
    Clone ID Related Accession (Same CDS sequence) XM_021474706.1
    Accession Version XM_021474706.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent T-type calcium channel subunit alpha-1H isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGCTGGTGA TTCTGCTGAA CTGTGTGACT CTGGGCATGT ATCAGCCATG TGAGGACCTC 
    AAGTGCCAGT CTGAGTGGTG TATCGTCCTG CAGGCGTTTG ATGACTGCAT CTTCGCCTTC
    TTCGCTGTGG AGATGGTCAT CAAAATGATC GCCCTGGGGA TTTTTGGAAT AAATGGTTAT
    CTTGGCGACA CGTGGAACCG CCTGGACTTC TTCATTGTCA TGGCAGGGAT GATGGAGTAT
    TCACTGGACG GTCATAACGC CAGCCTGTCA GCCATCCGGA CTGTGCGAGT GCTCAGGCCC
    CTGCGAGCCA TCAACAGAGT GCCCAGTATG AGGATACTGG TCACTCTGCT TCTAGATACT
    CTGCCTATGT TAGGCAACGT CCTCCTGCTG TGCTTTTTTG TCTTCTTTAT ATTCGGAATT
    GTCGGGGTGC AGCTGTGGGC TGGACTTCTT AGGAATCGGT GTTTCATGCC AAGCAAAGTG
    AGAGACCAAT ATAATCTGTC GTATATGAGT CTGTACTATG AGAACGAAGA TGGTGAAGAC
    AACCCCTTCA TCTGCTCCTC CAGTAAGGAC AACGGCATGA AGCGCTGCAG TGCTGTGCCA
    CCCCTTAAAG AGGGTGTTGA GTGCACTTTA AATGCATCTT TTCTGGGACA TGTCTACTCT
    GGCCTGAGCG GGACAGGCAA CTACAGCTGT GTGAACTGGA ACCAGTATTA CAGTGAATGC
    AAGCCCGGAG ACTTAAACCC GCACAAAGGC GCAGTAAACT TTGATAACAT TGGATATGCC
    TGGATCGCTA TATTCCAGGT TATTACATTG GAAGGATGGG TGGACATCAT GTATTACGTA
    ATGGATGCCC ACTCCTTTTA CAATTTTATA TATTTTATAT TGCTTATTAT AGTGGGTTCC
    TTCTTCATGA TCAACCTGTG CCTGGTTGTA ATAGCAACCC AGTTCGCAGA AACCAAACAG
    CGCGAGAACA TGCTGATGAA GGAGCAGCGT GCACGATACC GCTCCAACGA CAGCACCCTC
    GCCAGCTACT CTGAGCCGGG CAGCTGCTAC GAAGAGATGC TCAAGTACGT CAGCCACCTG
    TACCGCAAGG TGAAGCGCCG CCTCTCCCGC ATCTACAACA GCTGGCAGAG CAAGCGGCGA
    AAGAAAGTGA ACCCCAACAC CGGGGCTGGC GGAGGTGCCA ATGGTCACAG TAGGCACAGG
    GGTTACGGAC ACTGGGTACA GTCCATTCAC AGCCTCATCC AGCAGCACCA GCGACAGCAC
    TGTCACCTCA GCAATGGCAG CCCCAGTCCT GTGGCCACTA CGGGCTCCAG TGAGGCTCTG
    GAAATGAGGT CCATCACAGC GGGTCAGCAG CTGACCGTGT CCTCTCAGCC AACCGCCACG
    CACAATCTTT CTTCCAGCAC ACAATCAGTG CATAGCATTT ATCAAGGTGA CTTTCGTGAG
    GCCCCGCAGG AGCGAACACC CACTAATGTT GCTGTTCCGG CACTCAGCCG ACTCAATGGG
    GGGATGAACT ATCCCACAAT TTTGCCGTCC CTCATTTGCA ACTATTCAGG TGGAGGTTCA
    TTAGGGAAAG AGAGGGGGAA GTCGGATGCA AATACAGGAC ATGAGGTGAT ACAGGACTTT
    GACAAGCTGC ACCAGCAAAT TGAAGCACAT AGCCACAGTC GTGTGCTGCT GCAGATGGCT
    GGTGTGGTGC CCACTCAATT ACTTCTTGAG GTCATGAGCT GCCCGTATTG CGTAAGGGCC
    CTTGAGAGCA TGGATCTGGA GATTGGTGAT TCAGACTACC CTCGATCAGA AGGGGACATG
    GTGTATGAGT TCTCATATGA TGCCGGGTTC ATAACCGGCC GTTCAGCACA TCATAAAATG
    CAGGAGAAAC AAAACCGTCT GGTTCAATTC TGGGAGGACT TCAGGGAAAG ACTGACCCGT
    ATAGTGGATA GCAAATACTT TAACCGGGGA ATTATGATCG CCATCCTCAT CAACACCTTG
    AGTATGGGGA TTGAATACCA TGAGCAGCCC GAGGAGCTGA CCAACATCCT GGAGATCAGC
    AACATCGTCT TCACTAGCAT GTTTGTCCTC GAAATGCTCT TCAAACTGCT GGCCTTTGGC
    ATCTTCGGCT ATATAAGGAA CCCATATAAC ATCTTTGATG GGGTTATTGT TGTCATAAGT
    GTATGGGAGA TCATCGGTCA TGCGGATGGT GGTCTCTCAG TGCTGAGGAC TTTTCGTCTC
    CTGAGGGTGC TCAAACTGGT GCGCTTCCTC CCTGCCCTGC GCAGACAGCT GCTTGTCCTG
    ATGAAGACCA TGGACAATGT GGCAACATTT TGCATGCTAC TCATGCTCTT CATCTTCACT
    TTCAGCATAC TCGGAATGCA TCTGTTCGGA TGCAAATTCA GCCTGAAGAT GGAGAATGGA
    GACACTATTC CGGACAGGAA AAACTTTGAT TCCCTGCTGT GGGCCATTGT CACTGTGTTT
    CAGATTCTGA CCCAGGAAGA CTGGAACGTG GTCCTCTATA ATGGAATGGC TTCCACCAGC
    CCATGGGCGG CTCTTTATTT TGTTGCCCTC ATGACGTTTG GGAATTATGT GCTCTTTAAT
    CTACTGGTGG CCATTTTAGT GGAAGGGTTT CAGGCGGAGG GAGATGCTAA CAAGTCAGAC
    GGAGATGAGG AAAAGACATC AGTGAACTCT GAAGAGAAAA TGGAGCAGCT GCAAAGTTCA
    GAACCCCTTT CAATTTCTGA TCCAGACTTG AAGTTCTACT CTCTGATGTT GTCAGCCAAT
    GGGCATGTGG ATCCTTGTGG CACTTTGCCT CCACCAATCA TCATGCGCAC TGCTGCCACA
    CCCATGCCCA CTCCAAAGAG CTCTCTGGGG CCCGAGTCTG TGTTTGACTT GACCGAGTCC
    AGACGGGGCA GTGCTGTCTC CATAGATCCA AATGCTTTTG ATCAGAAGTC CCTGTCCAGT
    CCAAGAAGAT CACCATGCCA CCCCCGTGGC TCGGGCAGTA ACTGGGGCAG CCGCAGGTCG
    TCCTGGAACA GCTTAGGACG AGCGCCAAGT CTGAAAAGGA AGGACACATC TGGGGAGCGC
    GAGTCTCTGC TGTCCGGGGA AGGCAGGGGC AGCTTTGAGG ATGACGACAT AGAGGCGGAG
    AAGCTGACTA ACATCAGCGG AGGCTCCCTG CAGCACCCCA GCTCTCTGGA CATCCCAGAA
    CTGCCTCAAA TGGGGGAATA CCTCGACTGC AATGGACGAA GCCAACACAT ACTCGCTGAC
    CTGTCTGCCA GTCTCAACAA GGAGGACTCC ATCGCTGAGG AGGACTTGGA TGATAGCTTC
    TGTTTCCGTC TCAGGAGGAC CCTGGCAGCT TATAAACCCA AGTGGTGCAA AGATCATGAG
    GAATGGTCAC TTTACCTGTT CTCTCCTCAT AACAAGTTTC GGATGATGTG TCAAAAACTC
    ATCTCTCACA AGATGTTTGA TTATGTAGTT TTAGTATTCA TCTTCCTAAA CTGCATCACT
    ATTGCTCTGG AGAGACCCCA TATTCAACAA TCTGAGCGAT TATTTCTCCT CGTCTCAAAC
    TATGTTTTCA CTGTGATATT TGTTGCTGAA ATGACAGTGA AGGTTGTGGC CCTTGGTTTC
    TACTCCGGGA ACCAGAGTTA TCTAAAGAGC ACATGGAATG TTTTGGATGG GGTGCTGGTC
    TTTGTGTCCC TCATTGACAT TCTGGTCTCT CTGGCCTGGA CTGGAAACCG AATCTTTGGC
    ATCCTGAGGG TTCTTCGCCT GCTGCGAACT CTGCGGCCAC TGAGAGTAAT TAGTCGTGCT
    CCTGGTCTGA AGCTGGTAGT AGAAACGTTG ATAACGTCCC TGAGGCCCAT TGGGAACATT
    GTGCTGATCT GCTGTGCTTT CTTTATTGTT TTCGGCATTC TTGGAGTTCA GCTCTTCAAA
    GGAAAGTTTT TCCACTGTGA AGGCGGCGAT ACCAGAAACA TCACCAATAA GTCTGACTGT
    CTTCAGGCAA ACCTCAAATG GATCAGAAGA AAGTACAATT TTGACAACTT GGGACAGGCT
    CTCATGTCTC TTTTCGTGCT TTCCTGTAAG GATGGATGGG TCAATATAAT GTATGATGGG
    TTGGATGCTG TTGGTGTGGA CCAGCAGCCT GAGCGCAACC ACAACCCCTG GATGCTGTTA
    TATTTTATTT CCTTTCTGCT AATCGTCAGC TTCTTCGTCC TGAATATGTT TGTCGGAGTA
    GTGGTGGAAA ACTTCCACAA GTGCCGTCAA GACCAGGAGG AAGTGGAGGC GCGTCTGCTG
    GAGTTGAAGA GGCAAAAACT GATGGAGAAA AAGCGCAGGA GTAAGGAGAA TTGCGGGTCT
    GAGGCACTCC GGAGACCATA TTATGCTGAC TATTCTCCAG CCCGGCTTTA CATCCACACA
    CTGTGCACCA ACCACTATTT GGACCTGTTT ATAACCGGCA TCATCTGCAT CAACGTAGTT
    ACCATGTCCA TCGAGCATTT CAATCAGCCT TCTTATCTTG ACGAGGCACT TAAATACTGT
    AACTATGTAT TTACAATCAT CTTCATAATT GAGGCTCTGC TTAAACTCGT GGCATTTGGA
    ATACGGAGAT TCTTCAAAGA CAGATGGAAC CAGCTGGATC TTGCAATAGT CCTACTCTCT
    ATCATGGGCA TCACTCTGGA GGAGATTAAG ATGAATGCAG CGCTGCCCAT CAACCCCACC
    ATCATCCGTA TTATGAGAGT GCTAAGAATC GCAAGAGTGC TGAAGCTGTT AAAGATGGCT
    ACAGGCATGA GATCCCTACT CGACACCGTA ATGCAAGCTT TACCACAGGT GGGAAATCTG
    GGCTTGCTGT TTATGCTTCT CTTCTTCATT TATGCGGCGC TTGGCGTGGA GCTTTTTGGG
    AAACTAGAAT GCACAGAGAA CAATCCGTGT GAAGGATTAA GTCCACATGC CACGTTTGAG
    AACTTTGGAA TGGCATTTTT GACACTTTTC CGAGTATCAA CTGGGGACAA TTGGAATGGA
    ATAATGAAGG ACACTCTGCG AGAGTGCCTT CCTTCAGAGA CGCAATGCCT CAGCTATCTA
    CCCTGGGTCT CTCCAATCTA TTTTGTAACC TTTGTTCTAA TGGCTCAGTT TGTGCTGGTT
    AACGTGGTCG TAGCTGTGTT GATGAAACAT CTGGAGGAGA GCAATAAAGA AGCTAAAGAG
    GATGCAGAAA TGGATGCTGC GATCAAGTTG GCAATTTTGA AAGAAACTCG CCGTCTCAGC
    ACCATCTCAG CCAGTGCCAC TGCGGGACCA GAAGGCAGGG CAACTTATAT CGAGCCACAC
    CCAGATTCAC CTGAGCAGGA TGAGGGGATG CAAAGAACAC AGGGGAATTT GCTCTCTCCG
    CGGAAAATGT CAGTGTCAAG GATGCACTCT TTACCCAATG ACAGCTACAT GTTTCGCCCT
    GTGAGGCCAG CCAGTGCTCC GTATCCTCTA GAAGAAGTGG AGACCTGTCA AGATTATCTT
    GAATTAGGTT CCATCACCTC GGTTCACTCC CAGCCATGCG AAAGCCTTTC CCTGCTCCAG
    GTCCCTGGTG TTCCTCCCCA TTCCCAGTTC AACTGCCTCC CCAAGATCCC TCCTCCCATG
    CCCTCTCCAA CCCACAGCAT AGACAGGATT TTACGCAGAC AGAGGGCAAT TAAAAATGAC
    TCCATTGACA TGCATAATTT CGACTCCAAA GAAAATGTGG AAAGACAGAA AGGGATAACC
    ACAGGCAGCT TCAAGCTGCA TGTTCCTCAA ATCACCGGCA TCTCTCAGGG GTCTCCCTTG
    CTGCCACCAC GTGGACCTAG TGTCCACACC TTCCAGCAGC CGCACAGCCA GCACAATATC
    TCTTCTCGAC CTCCCAGTCT GGCCAGCAGC AGCAGAAGCA CCAGCCCCAG TGGCTCAATG
    TTTGACTCCG CTGACCCAAC CGATGAGGAG GTGCGCCACA TCAATAGCAC AGCCCGCCTG
    TGGATCGGGG TTCATGCAGA AGCAAGGAGT CACTCCCTCT CTCCTTACAG CACCTCAGGG
    ACGCTGCTTC CCATCCCCGT GAAAAACTGC AGCAGTGCTG CCAACTTAAC CATCAAAGAC
    CCCAAGAAGT GCTTCAGTGT GGACACTGAG GGTTTTCTGG AAAGACCCTA CGCTTCTGAT
    GACCATCGCA GGCACTCGAT TGAGATTTGC TCCTCAGGAG ACGATGGGCT GTTTGGTCAA
    GAACTCTGTG AAAAGAGGCA CGTTCCCACG CGTGGAAAGT CTTTGCACAT TCCCCGGAAG
    ACAAAGATGA GCCCTCCCTG CATCTCCATC GAGCCCCCAT TTGAAAAGGA TGTTTCCCCC
    TCTCATGCGT CCATGAAAAA GTTGTCGGAT AGCATGTTAC TGCGGAGGAG GACACCATCC
    TATGACCTGG CCCTGCAGCG GGACTCTCTG GACTTGCCGG AGAACCAGCT GTCAACCCAG
    CCACTCATCA AAGTAGAGCG ACATCCCTCT CACTGTGCCG AGTATCTGTC CCTCCCACAC
    CCCGCCCCAA TGAGCGGTCT GGCAGGCGTT TTGTGTGAAA GCACGCAGCC GCCCCCCTTT
    GATAGCAGAT ATGAAGAATC CACGGACTTC GGCTCTGTAA ACAGGACAGC TCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330381.1
    CDS485..7081
    Translation

    Target ORF information:

    RefSeq Version XM_021474706.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, T type, alpha 1H subunit a (cacna1ha), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474706.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGCTGGTGA TTCTGCTGAA CTGTGTGACT CTGGGCATGT ATCAGCCATG TGAGGACCTC 
    AAGTGCCAGT CTGAGTGGTG TATCGTCCTG CAGGCGTTTG ATGACTGCAT CTTCGCCTTC
    TTCGCTGTGG AGATGGTCAT CAAAATGATC GCCCTGGGGA TTTTTGGAAT AAATGGTTAT
    CTTGGCGACA CGTGGAACCG CCTGGACTTC TTCATTGTCA TGGCAGGGAT GATGGAGTAT
    TCACTGGACG GTCATAACGC CAGCCTGTCA GCCATCCGGA CTGTGCGAGT GCTCAGGCCC
    CTGCGAGCCA TCAACAGAGT GCCCAGTATG AGGATACTGG TCACTCTGCT TCTAGATACT
    CTGCCTATGT TAGGCAACGT CCTCCTGCTG TGCTTTTTTG TCTTCTTTAT ATTCGGAATT
    GTCGGGGTGC AGCTGTGGGC TGGACTTCTT AGGAATCGGT GTTTCATGCC AAGCAAAGTG
    AGAGACCAAT ATAATCTGTC GTATATGAGT CTGTACTATG AGAACGAAGA TGGTGAAGAC
    AACCCCTTCA TCTGCTCCTC CAGTAAGGAC AACGGCATGA AGCGCTGCAG TGCTGTGCCA
    CCCCTTAAAG AGGGTGTTGA GTGCACTTTA AATGCATCTT TTCTGGGACA TGTCTACTCT
    GGCCTGAGCG GGACAGGCAA CTACAGCTGT GTGAACTGGA ACCAGTATTA CAGTGAATGC
    AAGCCCGGAG ACTTAAACCC GCACAAAGGC GCAGTAAACT TTGATAACAT TGGATATGCC
    TGGATCGCTA TATTCCAGGT TATTACATTG GAAGGATGGG TGGACATCAT GTATTACGTA
    ATGGATGCCC ACTCCTTTTA CAATTTTATA TATTTTATAT TGCTTATTAT AGTGGGTTCC
    TTCTTCATGA TCAACCTGTG CCTGGTTGTA ATAGCAACCC AGTTCGCAGA AACCAAACAG
    CGCGAGAACA TGCTGATGAA GGAGCAGCGT GCACGATACC GCTCCAACGA CAGCACCCTC
    GCCAGCTACT CTGAGCCGGG CAGCTGCTAC GAAGAGATGC TCAAGTACGT CAGCCACCTG
    TACCGCAAGG TGAAGCGCCG CCTCTCCCGC ATCTACAACA GCTGGCAGAG CAAGCGGCGA
    AAGAAAGTGA ACCCCAACAC CGGGGCTGGC GGAGGTGCCA ATGGTCACAG TAGGCACAGG
    GGTTACGGAC ACTGGGTACA GTCCATTCAC AGCCTCATCC AGCAGCACCA GCGACAGCAC
    TGTCACCTCA GCAATGGCAG CCCCAGTCCT GTGGCCACTA CGGGCTCCAG TGAGGCTCTG
    GAAATGAGGT CCATCACAGC GGGTCAGCAG CTGACCGTGT CCTCTCAGCC AACCGCCACG
    CACAATCTTT CTTCCAGCAC ACAATCAGTG CATAGCATTT ATCAAGGTGA CTTTCGTGAG
    GCCCCGCAGG AGCGAACACC CACTAATGTT GCTGTTCCGG CACTCAGCCG ACTCAATGGG
    GGGATGAACT ATCCCACAAT TTTGCCGTCC CTCATTTGCA ACTATTCAGG TGGAGGTTCA
    TTAGGGAAAG AGAGGGGGAA GTCGGATGCA AATACAGGAC ATGAGGTGAT ACAGGACTTT
    GACAAGCTGC ACCAGCAAAT TGAAGCACAT AGCCACAGTC GTGTGCTGCT GCAGATGGCT
    GGTGTGGTGC CCACTCAATT ACTTCTTGAG GTCATGAGCT GCCCGTATTG CGTAAGGGCC
    CTTGAGAGCA TGGATCTGGA GATTGGTGAT TCAGACTACC CTCGATCAGA AGGGGACATG
    GTGTATGAGT TCTCATATGA TGCCGGGTTC ATAACCGGCC GTTCAGCACA TCATAAAATG
    CAGGAGAAAC AAAACCGTCT GGTTCAATTC TGGGAGGACT TCAGGGAAAG ACTGACCCGT
    ATAGTGGATA GCAAATACTT TAACCGGGGA ATTATGATCG CCATCCTCAT CAACACCTTG
    AGTATGGGGA TTGAATACCA TGAGCAGCCC GAGGAGCTGA CCAACATCCT GGAGATCAGC
    AACATCGTCT TCACTAGCAT GTTTGTCCTC GAAATGCTCT TCAAACTGCT GGCCTTTGGC
    ATCTTCGGCT ATATAAGGAA CCCATATAAC ATCTTTGATG GGGTTATTGT TGTCATAAGT
    GTATGGGAGA TCATCGGTCA TGCGGATGGT GGTCTCTCAG TGCTGAGGAC TTTTCGTCTC
    CTGAGGGTGC TCAAACTGGT GCGCTTCCTC CCTGCCCTGC GCAGACAGCT GCTTGTCCTG
    ATGAAGACCA TGGACAATGT GGCAACATTT TGCATGCTAC TCATGCTCTT CATCTTCACT
    TTCAGCATAC TCGGAATGCA TCTGTTCGGA TGCAAATTCA GCCTGAAGAT GGAGAATGGA
    GACACTATTC CGGACAGGAA AAACTTTGAT TCCCTGCTGT GGGCCATTGT CACTGTGTTT
    CAGATTCTGA CCCAGGAAGA CTGGAACGTG GTCCTCTATA ATGGAATGGC TTCCACCAGC
    CCATGGGCGG CTCTTTATTT TGTTGCCCTC ATGACGTTTG GGAATTATGT GCTCTTTAAT
    CTACTGGTGG CCATTTTAGT GGAAGGGTTT CAGGCGGAGG GAGATGCTAA CAAGTCAGAC
    GGAGATGAGG AAAAGACATC AGTGAACTCT GAAGAGAAAA TGGAGCAGCT GCAAAGTTCA
    GAACCCCTTT CAATTTCTGA TCCAGACTTG AAGTTCTACT CTCTGATGTT GTCAGCCAAT
    GGGCATGTGG ATCCTTGTGG CACTTTGCCT CCACCAATCA TCATGCGCAC TGCTGCCACA
    CCCATGCCCA CTCCAAAGAG CTCTCTGGGG CCCGAGTCTG TGTTTGACTT GACCGAGTCC
    AGACGGGGCA GTGCTGTCTC CATAGATCCA AATGCTTTTG ATCAGAAGTC CCTGTCCAGT
    CCAAGAAGAT CACCATGCCA CCCCCGTGGC TCGGGCAGTA ACTGGGGCAG CCGCAGGTCG
    TCCTGGAACA GCTTAGGACG AGCGCCAAGT CTGAAAAGGA AGGACACATC TGGGGAGCGC
    GAGTCTCTGC TGTCCGGGGA AGGCAGGGGC AGCTTTGAGG ATGACGACAT AGAGGCGGAG
    AAGCTGACTA ACATCAGCGG AGGCTCCCTG CAGCACCCCA GCTCTCTGGA CATCCCAGAA
    CTGCCTCAAA TGGGGGAATA CCTCGACTGC AATGGACGAA GCCAACACAT ACTCGCTGAC
    CTGTCTGCCA GTCTCAACAA GGAGGACTCC ATCGCTGAGG AGGACTTGGA TGATAGCTTC
    TGTTTCCGTC TCAGGAGGAC CCTGGCAGCT TATAAACCCA AGTGGTGCAA AGATCATGAG
    GAATGGTCAC TTTACCTGTT CTCTCCTCAT AACAAGTTTC GGATGATGTG TCAAAAACTC
    ATCTCTCACA AGATGTTTGA TTATGTAGTT TTAGTATTCA TCTTCCTAAA CTGCATCACT
    ATTGCTCTGG AGAGACCCCA TATTCAACAA TCTGAGCGAT TATTTCTCCT CGTCTCAAAC
    TATGTTTTCA CTGTGATATT TGTTGCTGAA ATGACAGTGA AGGTTGTGGC CCTTGGTTTC
    TACTCCGGGA ACCAGAGTTA TCTAAAGAGC ACATGGAATG TTTTGGATGG GGTGCTGGTC
    TTTGTGTCCC TCATTGACAT TCTGGTCTCT CTGGCCTGGA CTGGAAACCG AATCTTTGGC
    ATCCTGAGGG TTCTTCGCCT GCTGCGAACT CTGCGGCCAC TGAGAGTAAT TAGTCGTGCT
    CCTGGTCTGA AGCTGGTAGT AGAAACGTTG ATAACGTCCC TGAGGCCCAT TGGGAACATT
    GTGCTGATCT GCTGTGCTTT CTTTATTGTT TTCGGCATTC TTGGAGTTCA GCTCTTCAAA
    GGAAAGTTTT TCCACTGTGA AGGCGGCGAT ACCAGAAACA TCACCAATAA GTCTGACTGT
    CTTCAGGCAA ACCTCAAATG GATCAGAAGA AAGTACAATT TTGACAACTT GGGACAGGCT
    CTCATGTCTC TTTTCGTGCT TTCCTGTAAG GATGGATGGG TCAATATAAT GTATGATGGG
    TTGGATGCTG TTGGTGTGGA CCAGCAGCCT GAGCGCAACC ACAACCCCTG GATGCTGTTA
    TATTTTATTT CCTTTCTGCT AATCGTCAGC TTCTTCGTCC TGAATATGTT TGTCGGAGTA
    GTGGTGGAAA ACTTCCACAA GTGCCGTCAA GACCAGGAGG AAGTGGAGGC GCGTCTGCTG
    GAGTTGAAGA GGCAAAAACT GATGGAGAAA AAGCGCAGGA GTAAGGAGAA TTGCGGGTCT
    GAGGCACTCC GGAGACCATA TTATGCTGAC TATTCTCCAG CCCGGCTTTA CATCCACACA
    CTGTGCACCA ACCACTATTT GGACCTGTTT ATAACCGGCA TCATCTGCAT CAACGTAGTT
    ACCATGTCCA TCGAGCATTT CAATCAGCCT TCTTATCTTG ACGAGGCACT TAAATACTGT
    AACTATGTAT TTACAATCAT CTTCATAATT GAGGCTCTGC TTAAACTCGT GGCATTTGGA
    ATACGGAGAT TCTTCAAAGA CAGATGGAAC CAGCTGGATC TTGCAATAGT CCTACTCTCT
    ATCATGGGCA TCACTCTGGA GGAGATTAAG ATGAATGCAG CGCTGCCCAT CAACCCCACC
    ATCATCCGTA TTATGAGAGT GCTAAGAATC GCAAGAGTGC TGAAGCTGTT AAAGATGGCT
    ACAGGCATGA GATCCCTACT CGACACCGTA ATGCAAGCTT TACCACAGGT GGGAAATCTG
    GGCTTGCTGT TTATGCTTCT CTTCTTCATT TATGCGGCGC TTGGCGTGGA GCTTTTTGGG
    AAACTAGAAT GCACAGAGAA CAATCCGTGT GAAGGATTAA GTCCACATGC CACGTTTGAG
    AACTTTGGAA TGGCATTTTT GACACTTTTC CGAGTATCAA CTGGGGACAA TTGGAATGGA
    ATAATGAAGG ACACTCTGCG AGAGTGCCTT CCTTCAGAGA CGCAATGCCT CAGCTATCTA
    CCCTGGGTCT CTCCAATCTA TTTTGTAACC TTTGTTCTAA TGGCTCAGTT TGTGCTGGTT
    AACGTGGTCG TAGCTGTGTT GATGAAACAT CTGGAGGAGA GCAATAAAGA AGCTAAAGAG
    GATGCAGAAA TGGATGCTGC GATCAAGTTG GCAATTTTGA AAGAAACTCG CCGTCTCAGC
    ACCATCTCAG CCAGTGCCAC TGCGGGACCA GAAGGCAGGG CAACTTATAT CGAGCCACAC
    CCAGATTCAC CTGAGCAGGA TGAGGGGATG CAAAGAACAC AGGGGAATTT GCTCTCTCCG
    CGGAAAATGT CAGTGTCAAG GATGCACTCT TTACCCAATG ACAGCTACAT GTTTCGCCCT
    GTGAGGCCAG CCAGTGCTCC GTATCCTCTA GAAGAAGTGG AGACCTGTCA AGATTATCTT
    GAATTAGGTT CCATCACCTC GGTTCACTCC CAGCCATGCG AAAGCCTTTC CCTGCTCCAG
    GTCCCTGGTG TTCCTCCCCA TTCCCAGTTC AACTGCCTCC CCAAGATCCC TCCTCCCATG
    CCCTCTCCAA CCCACAGCAT AGACAGGATT TTACGCAGAC AGAGGGCAAT TAAAAATGAC
    TCCATTGACA TGCATAATTT CGACTCCAAA GAAAATGTGG AAAGACAGAA AGGGATAACC
    ACAGGCAGCT TCAAGCTGCA TGTTCCTCAA ATCACCGGCA TCTCTCAGGG GTCTCCCTTG
    CTGCCACCAC GTGGACCTAG TGTCCACACC TTCCAGCAGC CGCACAGCCA GCACAATATC
    TCTTCTCGAC CTCCCAGTCT GGCCAGCAGC AGCAGAAGCA CCAGCCCCAG TGGCTCAATG
    TTTGACTCCG CTGACCCAAC CGATGAGGAG GTGCGCCACA TCAATAGCAC AGCCCGCCTG
    TGGATCGGGG TTCATGCAGA AGCAAGGAGT CACTCCCTCT CTCCTTACAG CACCTCAGGG
    ACGCTGCTTC CCATCCCCGT GAAAAACTGC AGCAGTGCTG CCAACTTAAC CATCAAAGAC
    CCCAAGAAGT GCTTCAGTGT GGACACTGAG GGTTTTCTGG AAAGACCCTA CGCTTCTGAT
    GACCATCGCA GGCACTCGAT TGAGATTTGC TCCTCAGGAG ACGATGGGCT GTTTGGTCAA
    GAACTCTGTG AAAAGAGGCA CGTTCCCACG CGTGGAAAGT CTTTGCACAT TCCCCGGAAG
    ACAAAGATGA GCCCTCCCTG CATCTCCATC GAGCCCCCAT TTGAAAAGGA TGTTTCCCCC
    TCTCATGCGT CCATGAAAAA GTTGTCGGAT AGCATGTTAC TGCGGAGGAG GACACCATCC
    TATGACCTGG CCCTGCAGCG GGACTCTCTG GACTTGCCGG AGAACCAGCT GTCAACCCAG
    CCACTCATCA AAGTAGAGCG ACATCCCTCT CACTGTGCCG AGTATCTGTC CCTCCCACAC
    CCCGCCCCAA TGAGCGGTCT GGCAGGCGTT TTGTGTGAAA GCACGCAGCC GCCCCCCTTT
    GATAGCAGAT ATGAAGAATC CACGGACTTC GGCTCTGTAA ACAGGACAGC TCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141819
    Clone ID Related Accession (Same CDS sequence) XM_021474707.1
    Accession Version XM_021474707.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6273bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent T-type calcium channel subunit alpha-1H isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGGTGA TTCTGCTGAA CTGTGTGACT CTGGGCATGT ATCAGCCATG TGAGGACCTC 
    AAGTGCCAGT CTGAGTGGTG TATCGTCCTG CAGGCGTTTG ATGACTGCAT CTTCGCCTTC
    TTCGCTGTGG AGATGGTCAT CAAAATGATC GCCCTGGGGA TTTTTGGAAT AAATGGTTAT
    CTTGGCGACA CGTGGAACCG CCTGGACTTC TTCATTGTCA TGGCAGGGAT GATGGAGTAT
    TCACTGGACG GTCATAACGC CAGCCTGTCA GCCATCCGGA CTGTGCGAGT GCTCAGGCCC
    CTGCGAGCCA TCAACAGAGT GCCCAGTATG AGGATACTGG TCACTCTGCT TCTAGATACT
    CTGCCTATGT TAGGCAACGT CCTCCTGCTG TGCTTTTTTG TCTTCTTTAT ATTCGGAATT
    GTCGGGGTGC AGCTGTGGGC TGGACTTCTT AGGAATCGGT GTTTCATGCC AAGCAAAGTG
    AGAGACCAAT ATAATCTGTC GTATATGAGT CTGTACTATG AGAACGAAGA TGGTGAAGAC
    AACCCCTTCA TCTGCTCCTC CAGTAAGGAC AACGGCATGA AGCGCTGCAG TGCTGTGCCA
    CCCCTTAAAG AGGGTGTTGA GTGCACTTTA AATGCATCTT TTCTGGGACA TGTCTACTCT
    GGCCTGAGCG GGACAGGCAA CTACAGCTGT GTGAACTGGA ACCAGTATTA CAGTGAATGC
    AAGCCCGGAG ACTTAAACCC GCACAAAGGC GCAGTAAACT TTGATAACAT TGGATATGCC
    TGGATCGCTA TATTCCAGGT TATTACATTG GAAGGATGGG TGGACATCAT GTATTACGTA
    ATGGATGCCC ACTCCTTTTA CAATTTTATA TATTTTATAT TGCTTATTAT AGTGGGTTCC
    TTCTTCATGA TCAACCTGTG CCTGGTTGTA ATAGCAACCC AGTTCGCAGA AACCAAACAG
    CGCGAGAACA TGCTGATGAA GGAGCAGCGT GCACGATACC GCTCCAACGA CAGCACCCTC
    GCCAGCTACT CTGAGCCGGG CAGCTGCTAC GAAGAGATGC TCAAGTACGT CAGCCACCTG
    TACCGCAAGG TGAAGCGCCG CCTCTCCCGC ATCTACAACA GCTGGCAGAG CAAGCGGCGA
    AAGAAAGTGA ACCCCAACAC CGGGGCTGGC GGAGGTGCCA ATGGTCACAG TAGGCACAGG
    GGTTACGGAC ACTGGGTACA GTCCATTCAC AGCCTCATCC AGCAGCACCA GCGACAGCAC
    TGTCACCTCA GCAATGGCAG CCCCAGTCCT GTGGCCACTA CGGGCTCCAG TGAGGCTCTG
    GAAATGAGGT CCATCACAGC GGGTCAGCAG CTGACCGTGT CCTCTCAGCC AACCGCCACG
    CACAATCTTT CTTCCAGCAC ACAATCAGTG CATAGCATTT ATCAAGGTGA CTTTCGTGAG
    GCCCCGCAGG AGCGAACACC CACTAATGTT GCTGTTCCGG CACTCAGCCG ACTCAATGGG
    GGGATGAACT ATCCCACAAT TTTGCCGTCC CTCATTTGCA ACTATTCAGG TGGAGGTTCA
    TTAGGGAAAG AGAGGGGGAA GTCGGATGCA AATACAGGAC ATGAGGTGAT ACAGGACTTT
    GACAAGCTGC ACCAGCAAAT TGAAGCACAT AGCCACAGTC GTGTGCTGCT GCAGATGGCT
    GGTGTGGTGC CCACTCAATT ACTTCTTGAG GTCATGAGCT GCCCGTATTG CGTAAGGGCC
    CTTGAGAGCA TGGATCTGGA GATTGGTGAT TCAGACTACC CTCGATCAGA AGGGGACATG
    GTGTATGAGT TCTCATATGA TGCCGGGTTC ATAACCGGCC GTTCAGCACA TCATAAAATG
    CAGGAGAAAC AAAACCGTCT GGTTCAATTC TGGGAGGACT TCAGGGAAAG ACTGACCCGT
    ATAGTGGATA GCAAATACTT TAACCGGGGA ATTATGATCG CCATCCTCAT CAACACCTTG
    AGTATGGGGA TTGAATACCA TGAGCAGCCC GAGGAGCTGA CCAACATCCT GGAGATCAGC
    AACATCGTCT TCACTAGCAT GTTTGTCCTC GAAATGCTCT TCAAACTGCT GGCCTTTGGC
    ATCTTCGGCT ATATAAGGAA CCCATATAAC ATCTTTGATG GGGTTATTGT TGTCATAAGT
    GTATGGGAGA TCATCGGTCA TGCGGATGGT GGTCTCTCAG TGCTGAGGAC TTTTCGTCTC
    CTGAGGGTGC TCAAACTGGT GCGCTTCCTC CCTGCCCTGC GCAGACAGCT GCTTGTCCTG
    ATGAAGACCA TGGACAATGT GGCAACATTT TGCATGCTAC TCATGCTCTT CATCTTCACT
    TTCAGCATAC TCGGAATGCA TCTGTTCGGA TGCAAATTCA GCCTGAAGAT GGAGAATGGA
    GACACTATTC CGGACAGGAA AAACTTTGAT TCCCTGCTGT GGGCCATTGT CACTGTGTTT
    CAGATTCTGA CCCAGGAAGA CTGGAACGTG GTCCTCTATA ATGGAATGGC TTCCACCAGC
    CCATGGGCGG CTCTTTATTT TGTTGCCCTC ATGACGTTTG GGAATTATGT GCTCTTTAAT
    CTACTGGTGG CCATTTTAGT GGAAGGGTTT CAGGCGGAGG GAGATGCTAA CAAGTCAGAC
    GGAGATGAGG AAAAGACATC AGTGAACTCT GAAGAGAAAA TGGAGCAGCT GCAAAGTTCA
    GAACCCCTTT CAATTTCTGA TCCAGACTTG AAGTTCTACT CTCTGATGTT GTCAGCCAAT
    GGGCATGTGG ATCCTTGTGG CACTTTGCCT CCACCAATCA TCATGCGCAC TGCTGCCACA
    CCCATGCCCA CTCCAAAGAG CTCTCTGGGG CCCGAGTCTG TGTTTGACTT GACCGAGTCC
    AGACGGGGCA GTGCTGTCTC CATAGATCCA AATGCTTTTG ATCAGAAGTC CCTGTCCAGT
    CCAAGAAGAT CACCATGCCA CCCCCGTGGC TCGGGCAGTA ACTGGGGCAG CCGCAGGTCG
    TCCTGGAACA GCTTAGGACG AGCGCCAAGT CTGAAAAGGA AGGACACATC TGGGGAGCGC
    GAGTCTCTGC TGTCCGGGGA AGGCAGGGGC AGCTTTGAGG ATGACGACAT AGAGGCGGAG
    AAGCTGACTA ACATCAGCGG AGGCTCCCTG CAGCACCCCA GCTCTCTGGA CATCCCAGAA
    CTGCCTCAAA TGGGGGAATA CCTCGACTGC AATGGACGAA GCCAACACAT ACTCGCTGAC
    CTGTCTGCCA GTCTCAACAA GGAGGACTCC ATCGCTGAGG AGGACTTGGA TGATAGCTTC
    TGTTTCCGTC TCAGGAGGAC CCTGGCAGCT TATAAACCCA AGTGGTGCAA AGATCATGAG
    GAATGGTCAC TTTACCTGTT CTCTCCTCAT AACAAGTTTC GGATGATGTG TCAAAAACTC
    ATCTCTCACA AGATGTTTGA TTATGTAGTT TTAGTATTCA TCTTCCTAAA CTGCATCACT
    ATTGCTCTGG AGAGACCCCA TATTCAACAA TCTGAGCGAT TATTTCTCCT CGTCTCAAAC
    TATGTTTTCA CTGTGATATT TGTTGCTGAA ATGACAGTGA AGGTTGTGGC CCTTGGTTTC
    TACTCCGGGA ACCAGAGTTA TCTAAAGAGC ACATGGAATG TTTTGGATGG GGTGCTGGTC
    TTTGTGTCCC TCATTGACAT TCTGGTCTCT CTGGCCTGGA CTGGAAACCG AATCTTTGGC
    ATCCTGAGGG TTCTTCGCCT GCTGCGAACT CTGCGGCCAC TGAGAGTAAT TAGTCGTGCT
    CCTGGTCTGA AGCTGGTAGT AGAAACGTTG ATAACGTCCC TGAGGCCCAT TGGGAACATT
    GTGCTGATCT GCTGTGCTTT CTTTATTGTT TTCGGCATTC TTGGAGTTCA GCTCTTCAAA
    GGAAAGTTTT TCCACTGTGA AGGCGGCGAT ACCAGAAACA TCACCAATAA GTCTGACTGT
    CTTCAGGCAA ACCTCAAATG GATCAGAAGA AAGTACAATT TTGACAACTT GGGACAGGCT
    CTCATGTCTC TTTTCGTGCT TTCCTGTAAG GATGGATGGG TCAATATAAT GTATGATGGG
    TTGGATGCTG TTGGTGTGGA CCAGCAGCCT GAGCGCAACC ACAACCCCTG GATGCTGTTA
    TATTTTATTT CCTTTCTGCT AATCGTCAGC TTCTTCGTCC TGAATATGTT TGTCGGAGTA
    GTGGTGGAAA ACTTCCACAA GTGCCGTCAA GACCAGGAGG AAGTGGAGGC GCGTCTGCTG
    GAGTTGAAGA GGCAAAAACT GATGGAGAAA AAGCGCAGGA GTAAGGAGAA TTGCGGGTCT
    GAGGCACTCC GGAGACCATA TTATGCTGAC TATTCTCCAG CCCGGCTTTA CATCCACACA
    CTGTGCACCA ACCACTATTT GGACCTGTTT ATAACCGGCA TCATCTGCAT CAACGTAGTT
    ACCATGTCCA TCGAGCATTT CAATCAGCCT TCTTATCTTG ACGAGGCACT TAAATACTGT
    AACTATGTAT TTACAATCAT CTTCATAATT GAGGCTCTGC TTAAACTCGT GGCATTTGGA
    ATACGGAGAT TCTTCAAAGA CAGATGGAAC CAGCTGGATC TTGCAATAGT CCTACTCTCT
    ATCATGGGCA TCACTCTGGA GGAGATTAAG ATGAATGCAG CGCTGCCCAT CAACCCCACC
    ATCATCCGTA TTATGAGAGT GCTAAGAATC GCAAGAGTGC TGAAGCTGTT AAAGATGGCT
    ACAGGCATGA GATCCCTACT CGACACCGTA ATGCAAGCTT TACCACAGGT GGGAAATCTG
    GGCTTGCTGT TTATGCTTCT CTTCTTCATT TATGCGGCGC TTGGCGTGGA GCTTTTTGGG
    AAACTAGAAT GCACAGAGAA CAATCCGTGT GAAGGATTAA GTCCACATGC CACGTTTGAG
    AACTTTGGAA TGGCATTTTT GACACTTTTC CGAGTATCAA CTGGGGACAA TTGGAATGGA
    ATAATGAAGG ACACTCTGCG AGAGTGCCTT CCTTCAGAGA CGCAATGCCT CAGCTATCTA
    CCCTGGGTCT CTCCAATCTA TTTTGTAACC TTTGTTCTAA TGGCTCAGTT TGTGCTGGTT
    AACGTGGTCG TAGCTGTGTT GATGAAACAT CTGGAGGAGA GCAATAAAGA AGCTAAAGAG
    GATGCAGAAA TGGATGCTGC GATCAAGTTG GCAATTTTGA AAGAAACTCG CCGTCTCAGC
    ACCATCTCAG CCAGTGCCAC TGCGGGACCA GAAGGCAGGG CAACTTATAT CGAGCCACAC
    CCAGATTCAC CTGAGCAGAG GGCAATTAAA AATGACTCCA TTGACATGCA TAATTTCGAC
    TCCAAAGAAA ATGTGGAAAG ACAGAAAGGG ATAACCACAG GCAGCTTCAA GCTGCATGTT
    CCTCAAATCA CCGGCATCTC TCAGGGGTCT CCCTTGCTGC CACCACGTGG ACCTAGTGTC
    CACACCTTCC AGCAGCCGCA CAGCCAGCAC AATATCTCTT CTCGACCTCC CAGTCTGGCC
    AGCAGCAGCA GAAGCACCAG CCCCAGTGGC TCAATGTTTG ACTCCGCTGA CCCAACCGAT
    GAGGAGGTGC GCCACATCAA TAGCACAGCC CGCCTGTGGA TCGGGGTTCA TGCAGAAGCA
    AGGAGTCACT CCCTCTCTCC TTACAGCACC TCAGGGACGC TGCTTCCCAT CCCCGTGAAA
    AACTGCAGCA GTGCTGCCAA CTTAACCATC AAAGACCCCA AGAAGTGCTT CAGTGTGGAC
    ACTGAGGGTT TTCTGGAAAG ACCCTACGCT TCTGATGACC ATCGCAGGCA CTCGATTGAG
    ATTTGCTCCT CAGGAGACGA TGGGCTGTTT GGTCAAGAAC TCTGTGAAAA GAGGCACGTT
    CCCACGCGTG GAAAGTCTTT GCACATTCCC CGGAAGACAA AGATGAGCCC TCCCTGCATC
    TCCATCGAGC CCCCATTTGA AAAGGATGTT TCCCCCTCTC ATGCGTCCAT GAAAAAGTTG
    TCGGATAGCA TGTTACTGCG GAGGAGGACA CCATCCTATG ACCTGGCCCT GCAGCGGGAC
    TCTCTGGACT TGCCGGAGAA CCAGCTGTCA ACCCAGCCAC TCATCAAAGT AGAGCGACAT
    CCCTCTCACT GTGCCGAGTA TCTGTCCCTC CCACACCCCG CCCCAATGAG CGGTCTGGCA
    GGCGTTTTGT GTGAAAGCAC GCAGCCGCCC CCCTTTGATA GCAGATATGA AGAATCCACG
    GACTTCGGCT CTGTAAACAG GACAGCTCAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330382.1
    CDS498..6770
    Translation

    Target ORF information:

    RefSeq Version XM_021474707.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, T type, alpha 1H subunit a (cacna1ha), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474707.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGGTGA TTCTGCTGAA CTGTGTGACT CTGGGCATGT ATCAGCCATG TGAGGACCTC 
    AAGTGCCAGT CTGAGTGGTG TATCGTCCTG CAGGCGTTTG ATGACTGCAT CTTCGCCTTC
    TTCGCTGTGG AGATGGTCAT CAAAATGATC GCCCTGGGGA TTTTTGGAAT AAATGGTTAT
    CTTGGCGACA CGTGGAACCG CCTGGACTTC TTCATTGTCA TGGCAGGGAT GATGGAGTAT
    TCACTGGACG GTCATAACGC CAGCCTGTCA GCCATCCGGA CTGTGCGAGT GCTCAGGCCC
    CTGCGAGCCA TCAACAGAGT GCCCAGTATG AGGATACTGG TCACTCTGCT TCTAGATACT
    CTGCCTATGT TAGGCAACGT CCTCCTGCTG TGCTTTTTTG TCTTCTTTAT ATTCGGAATT
    GTCGGGGTGC AGCTGTGGGC TGGACTTCTT AGGAATCGGT GTTTCATGCC AAGCAAAGTG
    AGAGACCAAT ATAATCTGTC GTATATGAGT CTGTACTATG AGAACGAAGA TGGTGAAGAC
    AACCCCTTCA TCTGCTCCTC CAGTAAGGAC AACGGCATGA AGCGCTGCAG TGCTGTGCCA
    CCCCTTAAAG AGGGTGTTGA GTGCACTTTA AATGCATCTT TTCTGGGACA TGTCTACTCT
    GGCCTGAGCG GGACAGGCAA CTACAGCTGT GTGAACTGGA ACCAGTATTA CAGTGAATGC
    AAGCCCGGAG ACTTAAACCC GCACAAAGGC GCAGTAAACT TTGATAACAT TGGATATGCC
    TGGATCGCTA TATTCCAGGT TATTACATTG GAAGGATGGG TGGACATCAT GTATTACGTA
    ATGGATGCCC ACTCCTTTTA CAATTTTATA TATTTTATAT TGCTTATTAT AGTGGGTTCC
    TTCTTCATGA TCAACCTGTG CCTGGTTGTA ATAGCAACCC AGTTCGCAGA AACCAAACAG
    CGCGAGAACA TGCTGATGAA GGAGCAGCGT GCACGATACC GCTCCAACGA CAGCACCCTC
    GCCAGCTACT CTGAGCCGGG CAGCTGCTAC GAAGAGATGC TCAAGTACGT CAGCCACCTG
    TACCGCAAGG TGAAGCGCCG CCTCTCCCGC ATCTACAACA GCTGGCAGAG CAAGCGGCGA
    AAGAAAGTGA ACCCCAACAC CGGGGCTGGC GGAGGTGCCA ATGGTCACAG TAGGCACAGG
    GGTTACGGAC ACTGGGTACA GTCCATTCAC AGCCTCATCC AGCAGCACCA GCGACAGCAC
    TGTCACCTCA GCAATGGCAG CCCCAGTCCT GTGGCCACTA CGGGCTCCAG TGAGGCTCTG
    GAAATGAGGT CCATCACAGC GGGTCAGCAG CTGACCGTGT CCTCTCAGCC AACCGCCACG
    CACAATCTTT CTTCCAGCAC ACAATCAGTG CATAGCATTT ATCAAGGTGA CTTTCGTGAG
    GCCCCGCAGG AGCGAACACC CACTAATGTT GCTGTTCCGG CACTCAGCCG ACTCAATGGG
    GGGATGAACT ATCCCACAAT TTTGCCGTCC CTCATTTGCA ACTATTCAGG TGGAGGTTCA
    TTAGGGAAAG AGAGGGGGAA GTCGGATGCA AATACAGGAC ATGAGGTGAT ACAGGACTTT
    GACAAGCTGC ACCAGCAAAT TGAAGCACAT AGCCACAGTC GTGTGCTGCT GCAGATGGCT
    GGTGTGGTGC CCACTCAATT ACTTCTTGAG GTCATGAGCT GCCCGTATTG CGTAAGGGCC
    CTTGAGAGCA TGGATCTGGA GATTGGTGAT TCAGACTACC CTCGATCAGA AGGGGACATG
    GTGTATGAGT TCTCATATGA TGCCGGGTTC ATAACCGGCC GTTCAGCACA TCATAAAATG
    CAGGAGAAAC AAAACCGTCT GGTTCAATTC TGGGAGGACT TCAGGGAAAG ACTGACCCGT
    ATAGTGGATA GCAAATACTT TAACCGGGGA ATTATGATCG CCATCCTCAT CAACACCTTG
    AGTATGGGGA TTGAATACCA TGAGCAGCCC GAGGAGCTGA CCAACATCCT GGAGATCAGC
    AACATCGTCT TCACTAGCAT GTTTGTCCTC GAAATGCTCT TCAAACTGCT GGCCTTTGGC
    ATCTTCGGCT ATATAAGGAA CCCATATAAC ATCTTTGATG GGGTTATTGT TGTCATAAGT
    GTATGGGAGA TCATCGGTCA TGCGGATGGT GGTCTCTCAG TGCTGAGGAC TTTTCGTCTC
    CTGAGGGTGC TCAAACTGGT GCGCTTCCTC CCTGCCCTGC GCAGACAGCT GCTTGTCCTG
    ATGAAGACCA TGGACAATGT GGCAACATTT TGCATGCTAC TCATGCTCTT CATCTTCACT
    TTCAGCATAC TCGGAATGCA TCTGTTCGGA TGCAAATTCA GCCTGAAGAT GGAGAATGGA
    GACACTATTC CGGACAGGAA AAACTTTGAT TCCCTGCTGT GGGCCATTGT CACTGTGTTT
    CAGATTCTGA CCCAGGAAGA CTGGAACGTG GTCCTCTATA ATGGAATGGC TTCCACCAGC
    CCATGGGCGG CTCTTTATTT TGTTGCCCTC ATGACGTTTG GGAATTATGT GCTCTTTAAT
    CTACTGGTGG CCATTTTAGT GGAAGGGTTT CAGGCGGAGG GAGATGCTAA CAAGTCAGAC
    GGAGATGAGG AAAAGACATC AGTGAACTCT GAAGAGAAAA TGGAGCAGCT GCAAAGTTCA
    GAACCCCTTT CAATTTCTGA TCCAGACTTG AAGTTCTACT CTCTGATGTT GTCAGCCAAT
    GGGCATGTGG ATCCTTGTGG CACTTTGCCT CCACCAATCA TCATGCGCAC TGCTGCCACA
    CCCATGCCCA CTCCAAAGAG CTCTCTGGGG CCCGAGTCTG TGTTTGACTT GACCGAGTCC
    AGACGGGGCA GTGCTGTCTC CATAGATCCA AATGCTTTTG ATCAGAAGTC CCTGTCCAGT
    CCAAGAAGAT CACCATGCCA CCCCCGTGGC TCGGGCAGTA ACTGGGGCAG CCGCAGGTCG
    TCCTGGAACA GCTTAGGACG AGCGCCAAGT CTGAAAAGGA AGGACACATC TGGGGAGCGC
    GAGTCTCTGC TGTCCGGGGA AGGCAGGGGC AGCTTTGAGG ATGACGACAT AGAGGCGGAG
    AAGCTGACTA ACATCAGCGG AGGCTCCCTG CAGCACCCCA GCTCTCTGGA CATCCCAGAA
    CTGCCTCAAA TGGGGGAATA CCTCGACTGC AATGGACGAA GCCAACACAT ACTCGCTGAC
    CTGTCTGCCA GTCTCAACAA GGAGGACTCC ATCGCTGAGG AGGACTTGGA TGATAGCTTC
    TGTTTCCGTC TCAGGAGGAC CCTGGCAGCT TATAAACCCA AGTGGTGCAA AGATCATGAG
    GAATGGTCAC TTTACCTGTT CTCTCCTCAT AACAAGTTTC GGATGATGTG TCAAAAACTC
    ATCTCTCACA AGATGTTTGA TTATGTAGTT TTAGTATTCA TCTTCCTAAA CTGCATCACT
    ATTGCTCTGG AGAGACCCCA TATTCAACAA TCTGAGCGAT TATTTCTCCT CGTCTCAAAC
    TATGTTTTCA CTGTGATATT TGTTGCTGAA ATGACAGTGA AGGTTGTGGC CCTTGGTTTC
    TACTCCGGGA ACCAGAGTTA TCTAAAGAGC ACATGGAATG TTTTGGATGG GGTGCTGGTC
    TTTGTGTCCC TCATTGACAT TCTGGTCTCT CTGGCCTGGA CTGGAAACCG AATCTTTGGC
    ATCCTGAGGG TTCTTCGCCT GCTGCGAACT CTGCGGCCAC TGAGAGTAAT TAGTCGTGCT
    CCTGGTCTGA AGCTGGTAGT AGAAACGTTG ATAACGTCCC TGAGGCCCAT TGGGAACATT
    GTGCTGATCT GCTGTGCTTT CTTTATTGTT TTCGGCATTC TTGGAGTTCA GCTCTTCAAA
    GGAAAGTTTT TCCACTGTGA AGGCGGCGAT ACCAGAAACA TCACCAATAA GTCTGACTGT
    CTTCAGGCAA ACCTCAAATG GATCAGAAGA AAGTACAATT TTGACAACTT GGGACAGGCT
    CTCATGTCTC TTTTCGTGCT TTCCTGTAAG GATGGATGGG TCAATATAAT GTATGATGGG
    TTGGATGCTG TTGGTGTGGA CCAGCAGCCT GAGCGCAACC ACAACCCCTG GATGCTGTTA
    TATTTTATTT CCTTTCTGCT AATCGTCAGC TTCTTCGTCC TGAATATGTT TGTCGGAGTA
    GTGGTGGAAA ACTTCCACAA GTGCCGTCAA GACCAGGAGG AAGTGGAGGC GCGTCTGCTG
    GAGTTGAAGA GGCAAAAACT GATGGAGAAA AAGCGCAGGA GTAAGGAGAA TTGCGGGTCT
    GAGGCACTCC GGAGACCATA TTATGCTGAC TATTCTCCAG CCCGGCTTTA CATCCACACA
    CTGTGCACCA ACCACTATTT GGACCTGTTT ATAACCGGCA TCATCTGCAT CAACGTAGTT
    ACCATGTCCA TCGAGCATTT CAATCAGCCT TCTTATCTTG ACGAGGCACT TAAATACTGT
    AACTATGTAT TTACAATCAT CTTCATAATT GAGGCTCTGC TTAAACTCGT GGCATTTGGA
    ATACGGAGAT TCTTCAAAGA CAGATGGAAC CAGCTGGATC TTGCAATAGT CCTACTCTCT
    ATCATGGGCA TCACTCTGGA GGAGATTAAG ATGAATGCAG CGCTGCCCAT CAACCCCACC
    ATCATCCGTA TTATGAGAGT GCTAAGAATC GCAAGAGTGC TGAAGCTGTT AAAGATGGCT
    ACAGGCATGA GATCCCTACT CGACACCGTA ATGCAAGCTT TACCACAGGT GGGAAATCTG
    GGCTTGCTGT TTATGCTTCT CTTCTTCATT TATGCGGCGC TTGGCGTGGA GCTTTTTGGG
    AAACTAGAAT GCACAGAGAA CAATCCGTGT GAAGGATTAA GTCCACATGC CACGTTTGAG
    AACTTTGGAA TGGCATTTTT GACACTTTTC CGAGTATCAA CTGGGGACAA TTGGAATGGA
    ATAATGAAGG ACACTCTGCG AGAGTGCCTT CCTTCAGAGA CGCAATGCCT CAGCTATCTA
    CCCTGGGTCT CTCCAATCTA TTTTGTAACC TTTGTTCTAA TGGCTCAGTT TGTGCTGGTT
    AACGTGGTCG TAGCTGTGTT GATGAAACAT CTGGAGGAGA GCAATAAAGA AGCTAAAGAG
    GATGCAGAAA TGGATGCTGC GATCAAGTTG GCAATTTTGA AAGAAACTCG CCGTCTCAGC
    ACCATCTCAG CCAGTGCCAC TGCGGGACCA GAAGGCAGGG CAACTTATAT CGAGCCACAC
    CCAGATTCAC CTGAGCAGAG GGCAATTAAA AATGACTCCA TTGACATGCA TAATTTCGAC
    TCCAAAGAAA ATGTGGAAAG ACAGAAAGGG ATAACCACAG GCAGCTTCAA GCTGCATGTT
    CCTCAAATCA CCGGCATCTC TCAGGGGTCT CCCTTGCTGC CACCACGTGG ACCTAGTGTC
    CACACCTTCC AGCAGCCGCA CAGCCAGCAC AATATCTCTT CTCGACCTCC CAGTCTGGCC
    AGCAGCAGCA GAAGCACCAG CCCCAGTGGC TCAATGTTTG ACTCCGCTGA CCCAACCGAT
    GAGGAGGTGC GCCACATCAA TAGCACAGCC CGCCTGTGGA TCGGGGTTCA TGCAGAAGCA
    AGGAGTCACT CCCTCTCTCC TTACAGCACC TCAGGGACGC TGCTTCCCAT CCCCGTGAAA
    AACTGCAGCA GTGCTGCCAA CTTAACCATC AAAGACCCCA AGAAGTGCTT CAGTGTGGAC
    ACTGAGGGTT TTCTGGAAAG ACCCTACGCT TCTGATGACC ATCGCAGGCA CTCGATTGAG
    ATTTGCTCCT CAGGAGACGA TGGGCTGTTT GGTCAAGAAC TCTGTGAAAA GAGGCACGTT
    CCCACGCGTG GAAAGTCTTT GCACATTCCC CGGAAGACAA AGATGAGCCC TCCCTGCATC
    TCCATCGAGC CCCCATTTGA AAAGGATGTT TCCCCCTCTC ATGCGTCCAT GAAAAAGTTG
    TCGGATAGCA TGTTACTGCG GAGGAGGACA CCATCCTATG ACCTGGCCCT GCAGCGGGAC
    TCTCTGGACT TGCCGGAGAA CCAGCTGTCA ACCCAGCCAC TCATCAAAGT AGAGCGACAT
    CCCTCTCACT GTGCCGAGTA TCTGTCCCTC CCACACCCCG CCCCAATGAG CGGTCTGGCA
    GGCGTTTTGT GTGAAAGCAC GCAGCCGCCC CCCTTTGATA GCAGATATGA AGAATCCACG
    GACTTCGGCT CTGTAAACAG GACAGCTCAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43366
    Clone ID Related Accession (Same CDS sequence) XM_009299684.3 , XM_009299684.2
    Accession Version XM_009299684.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent T-type calcium channel subunit alpha-1H isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299684.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGCTGGTGA TTCTGCTGAA CTGTGTGACT CTGGGCATGT ATCAGCCATG TGAGGACCTC 
    AAGTGCCAGT CTGAGTGGTG TATCGTCCTG CAGGCGTTTG ATGACTGCAT CTTCGCCTTC
    TTCGCTGTGG AGATGGTCAT CAAAATGATC GCCCTGGGGA TTTTTGGAAT AAATGGTTAT
    CTTGGCGACA CGTGGAACCG CCTGGACTTC TTCATTGTCA TGGCAGGGAT GATGGAGTAT
    TCACTGGACG GTCATAACGC CAGCCTGTCA GCCATCCGGA CTGTGCGAGT GCTCAGGCCC
    CTGCGAGCCA TCAACAGAGT GCCCAGTATG AGGATACTGG TCACTCTGCT TCTAGATACT
    CTGCCTATGT TAGGCAACGT CCTCCTGCTG TGCTTTTTTG TCTTCTTTAT ATTCGGAATT
    GTCGGGGTGC AGCTGTGGGC TGGACTTCTT AGGAATCGGT GTTTCATGCC AAGCAAAGTG
    AGAGACCAAT ATAATCTGTC GTATATGAGT CTGTACTATG AGAACGAAGA TGGTGAAGAC
    AACCCCTTCA TCTGCTCCTC CAGTAAGGAC AACGGCATGA AGCGCTGCAG TGCTGTGCCA
    CCCCTTAAAG AGGGTGTTGA GTGCACTTTA AATGCATCTT TTCTGGGACA TGTCTACTCT
    GGCCTGAGCG GGACAGGCAA CTACAGCTGT GTGAACTGGA ACCAGTATTA CAGTGAATGC
    AAGCCCGGAG ACTTAAACCC GCACAAAGGC GCAGTAAACT TTGATAACAT TGGATATGCC
    TGGATCGCTA TATTCCAGGT TATTACATTG GAAGGATGGG TGGACATCAT GTATTACGTA
    ATGGATGCCC ACTCCTTTTA CAATTTTATA TATTTTATAT TGCTTATTAT AGTGGGTTCC
    TTCTTCATGA TCAACCTGTG CCTGGTTGTA ATAGCAACCC AGTTCGCAGA AACCAAACAG
    CGCGAGAACA TGCTGATGAA GGAGCAGCGT GCACGATACC GCTCCAACGA CAGCACCCTC
    GCCAGCTACT CTGAGCCGGG CAGCTGCTAC GAAGAGATGC TCAAGTACGT CAGCCACCTG
    TACCGCAAGG TGAAGCGCCG CCTCTCCCGC ATCTACAACA GCTGGCAGAG CAAGCGGCGA
    AAGAAAGTGA ACCCCAACAC CGGGGCTGGC GGAGGTGCCA ATGGTCACAG TAGGCACAGG
    GGTTACGGAC ACTGGGTACA GTCCATTCAC AGCCTCATCC AGCAGCACCA GCGACAGCAC
    TGTCACCTCA GCAATGGCAG CCCCAGTCCT GTGGCCACTA CGGGCTCCAG TGAGGCTCTG
    GAAATGAGGT CCATCACAGC GGGTCAGCAG CTGACCGTGT CCTCTCAGCC AACCGCCACG
    CACAATCTTT CTTCCAGCAC ACAATCAGTG CATAGCATTT ATCAAGGTGA CTTTCGTGAG
    GCCCCGCAGG AGCGAACACC CACTAATGTT GCTGTTCCGG CACTCAGCCG ACTCAATGGG
    GGGATGAACT ATCCCACAAT TTTGCCGTCC CTCATTTGCA ACTATTCAGG TGGAGGTTCA
    TTAGGGAAAG AGAGGGGGAA GTCGGATGCA AATACAGGAC ATGAGGTGAT ACAGGACTTT
    GACAAGCTGC ACCAGCAAAT TGAAGCACAT AGCCACAGTC GTGTGCTGCT GCAGATGGCT
    GGTGTGGTGC CCACTCAATT ACTTCTTGAG GTCATGAGCT GCCCGTATTG CGTAAGGGCC
    CTTGAGAGCA TGGATCTGGA GATTGGTGAT TCAGACTACC CTCGATCAGA AGGGGACATG
    GTGTATGAGT TCTCATATGA TGCCGGGTTC ATAACCGGCC GTTCAGCACA TCATAAAATG
    CAGGAGAAAC AAAACCGTCT GGTTCAATTC TGGGAGGACT TCAGGGAAAG ACTGACCCGT
    ATAGTGGATA GCAAATACTT TAACCGGGGA ATTATGATCG CCATCCTCAT CAACACCTTG
    AGTATGGGGA TTGAATACCA TGAGCAGCCC GAGGAGCTGA CCAACATCCT GGAGATCAGC
    AACATCGTCT TCACTAGCAT GTTTGTCCTC GAAATGCTCT TCAAACTGCT GGCCTTTGGC
    ATCTTCGGCT ATATAAGGAA CCCATATAAC ATCTTTGATG GGGTTATTGT TGTCATAAGT
    GTATGGGAGA TCATCGGTCA TGCGGATGGT GGTCTCTCAG TGCTGAGGAC TTTTCGTCTC
    CTGAGGGTGC TCAAACTGGT GCGCTTCCTC CCTGCCCTGC GCAGACAGCT GCTTGTCCTG
    ATGAAGACCA TGGACAATGT GGCAACATTT TGCATGCTAC TCATGCTCTT CATCTTCACT
    TTCAGCATAC TCGGAATGCA TCTGTTCGGA TGCAAATTCA GCCTGAAGAT GGAGAATGGA
    GACACTATTC CGGACAGGAA AAACTTTGAT TCCCTGCTGT GGGCCATTGT CACTGTGTTT
    CAGATTCTGA CCCAGGAAGA CTGGAACGTG GTCCTCTATA ATGGAATGGC TTCCACCAGC
    CCATGGGCGG CTCTTTATTT TGTTGCCCTC ATGACGTTTG GGAATTATGT GCTCTTTAAT
    CTACTGGTGG CCATTTTAGT GGAAGGGTTT CAGGCGGAGG GAGATGCTAA CAAGTCAGAC
    GGAGATGAGG AAAAGACATC AGTGAACTCT GAAGAGAAAA TGGAGCAGCT GCAAAGTTCA
    GAACCCCTTT CAATTTCTGA TCCAGACTTG AAGTTCTACT CTCTGATGTT GTCAGCCAAT
    GGGCATGTGG ATCCTTGTGG CACTTTGCCT CCACCAATCA TCATGCGCAC TGCTGCCACA
    CCCATGCCCA CTCCAAAGAG CTCTCTGGGG CCCGAGTCTG TGTTTGACTT GACCGAGTCC
    AGACGGGGCA GTGCTGTCTC CATAGATCCA AATGCTTTTG ATCAGAAGTC CCTGTCCAGT
    CCAAGAAGAT CACCATGCCA CCCCCGTGGC TCGGGCAGTA ACTGGGGCAG CCGCAGGTCG
    TCCTGGAACA GCTTAGGACG AGCGCCAAGT CTGAAAAGGA AGGACACATC TGGGGAGCGC
    GAGTCTCTGC TGTCCGGGGA AGGCAGGGGC AGCTTTGAGG ATGACGACAT AGAGGCGGAG
    AAGCTGACTA ACATCAGCGG AGGCTCCCTG CAGCACCCCA GCTCTCTGGA CATCCCAGAA
    CTGCCTCAAA TGGGGGAATA CCTCGACTGC AATGGACGAA GCCAACACAT ACTCGCTGAC
    CTGTCTGCCA GTCTCAACAA GGAGGACTCC ATCGCTGAGG AGGACTTGGA TGATAGCTTC
    TGTTTCCGTC TCAGGAGGAC CCTGGCAGCT TATAAACCCA AGTGGTGCAA AGATCATGAG
    GAATGGTCAC TTTACCTGTT CTCTCCTCAT AACAAGTTTC GGATGATGTG TCAAAAACTC
    ATCTCTCACA AGATGTTTGA TTATGTAGTT TTAGTATTCA TCTTCCTAAA CTGCATCACT
    ATTGCTCTGG AGAGACCCCA TATTCAACAA TCTGAGCGAT TATTTCTCCT CGTCTCAAAC
    TATGTTTTCA CTGTGATATT TGTTGCTGAA ATGACAGTGA AGGTTGTGGC CCTTGGTTTC
    TACTCCGGGA ACCAGAGTTA TCTAAAGAGC ACATGGAATG TTTTGGATGG GGTGCTGGTC
    TTTGTGTCCC TCATTGACAT TCTGGTCTCT CTGGCCTGGA CTGGAAACCG AATCTTTGGC
    ATCCTGAGGG TTCTTCGCCT GCTGCGAACT CTGCGGCCAC TGAGAGTAAT TAGTCGTGCT
    CCTGGTCTGA AGCTGGTAGT AGAAACGTTG ATAACGTCCC TGAGGCCCAT TGGGAACATT
    GTGCTGATCT GCTGTGCTTT CTTTATTGTT TTCGGCATTC TTGGAGTTCA GCTCTTCAAA
    GGAAAGTTTT TCCACTGTGA AGGCGGCGAT ACCAGAAACA TCACCAATAA GTCTGACTGT
    CTTCAGGCAA ACCTCAAATG GATCAGAAGA AAGTACAATT TTGACAACTT GGGACAGGCT
    CTCATGTCTC TTTTCGTGCT TTCCTGTAAG GATGGATGGG TCAATATAAT GTATGATGGG
    TTGGATGCTG TTGGTGTGGA CCAGCAGCCT GAGCGCAACC ACAACCCCTG GATGCTGTTA
    TATTTTATTT CCTTTCTGCT AATCGTCAGC TTCTTCGTCC TGAATATGTT TGTCGGAGTA
    GTGGTGGAAA ACTTCCACAA GTGCCGTCAA GACCAGGAGG AAGTGGAGGC GCGTCTGCTG
    GAGTTGAAGA GGCAAAAACT GATGGAGAAA AAGCGCAGGA AGGCACTCCG GAGACCATAT
    TATGCTGACT ATTCTCCAGC CCGGCTTTAC ATCCACACAC TGTGCACCAA CCACTATTTG
    GACCTGTTTA TAACCGGCAT CATCTGCATC AACGTAGTTA CCATGTCCAT CGAGCATTTC
    AATCAGCCTT CTTATCTTGA CGAGGCACTT AAATACTGTA ACTATGTATT TACAATCATC
    TTCATAATTG AGGCTCTGCT TAAACTCGTG GCATTTGGAA TACGGAGATT CTTCAAAGAC
    AGATGGAACC AGCTGGATCT TGCAATAGTC CTACTCTCTA TCATGGGCAT CACTCTGGAG
    GAGATTAAGA TGAATGCAGC GCTGCCCATC AACCCCACCA TCATCCGTAT TATGAGAGTG
    CTAAGAATCG CAAGAGTGCT GAAGCTGTTA AAGATGGCTA CAGGCATGAG ATCCCTACTC
    GACACCGTAA TGCAAGCTTT ACCACAGGTG GGAAATCTGG GCTTGCTGTT TATGCTTCTC
    TTCTTCATTT ATGCGGCGCT TGGCGTGGAG CTTTTTGGGA AACTAGAATG CACAGAGAAC
    AATCCGTGTG AAGGATTAAG TCCACATGCC ACGTTTGAGA ACTTTGGAAT GGCATTTTTG
    ACACTTTTCC GAGTATCAAC TGGGGACAAT TGGAATGGAA TAATGAAGGA CACTCTGCGA
    GAGTGCCTTC CTTCAGAGAC GCAATGCCTC AGCTATCTAC CCTGGGTCTC TCCAATCTAT
    TTTGTAACCT TTGTTCTAAT GGCTCAGTTT GTGCTGGTTA ACGTGGTCGT AGCTGTGTTG
    ATGAAACATC TGGAGGAGAG CAATAAAGAA GCTAAAGAGG ATGCAGAAAT GGATGCTGCG
    ATCAAGTTGG CAATTTTGAA AGAAACTCGC CGTCTCAGCA CCATCTCAGC CAGTGCCACT
    GCGGGACCAG AAGGCAGGGC AACTTATATC GAGCCACACC CAGATTCACC TGAGCAGGAT
    GAGGGGATGC AAAGAACACA GGGGAATTTG CTCTCTCCGC GGAAAATGTC AGTGTCAAGG
    ATGCACTCTT TACCCAATGA CAGCTACATG TTTCGCCCTG TGAGGCCAGC CAGTGCTCCG
    TATCCTCTAG AAGAAGTGGA GACCTGTCAA GATTATCTTG AATTAGGTTC CATCACCTCG
    GTTCACTCCC AGCCATGCGA AAGCCTTTCC CTGCTCCAGG TCCCTGGTGT TCCTCCCCAT
    TCCCAGTTCA ACTGCCTCCC CAAGATCCCT CCTCCCATGC CCTCTCCAAC CCACAGCATA
    GACAGGATTT TACGCAGACA GAGGGCAATT AAAAATGACT CCATTGACAT GCATAATTTC
    GACTCCAAAG AAAATGTGGA AAGACAGAAA GGGATAACCA CAGGCAGCTT CAAGCTGCAT
    GTTCCTCAAA TCACCGGCAT CTCTCAGGGG TCTCCCTTGC TGCCACCACG TGGACCTAGT
    GTCCACACCT TCCAGCAGCC GCACAGCCAG CACAATATCT CTTCTCGACC TCCCAGTCTG
    GCCAGCAGCA GCAGAAGCAC CAGCCCCAGT GGCTCAATGT TTGACTCCGC TGACCCAACC
    GATGAGGAGG TGCGCCACAT CAATAGCACA GCCCGCCTGT GGATCGGGGT TCATGCAGAA
    GCAAGGAGTC ACTCCCTCTC TCCTTACAGC ACCTCAGGGA CGCTGCTTCC CATCCCCGTG
    AAAAACTGCA GCAGTGCTGC CAACTTAACC ATCAAAGACC CCAAGAAGTG CTTCAGTGTG
    GACACTGAGG GTTTTCTGGA AAGACCCTAC GCTTCTGATG ACCATCGCAG GCACTCGATT
    GAGATTTGCT CCTCAGGAGA CGATGGGCTG TTTGGTCAAG AACTCTGTGA AAAGAGGCAC
    GTTCCCACGC GTGGAAAGTC TTTGCACATT CCCCGGAAGA CAAAGATGAG CCCTCCCTGC
    ATCTCCATCG AGCCCCCATT TGAAAAGGAT GTTTCCCCCT CTCATGCGTC CATGAAAAAG
    TTGTCGGATA GCATGTTACT GCGGAGGAGG ACACCATCCT ATGACCTGGC CCTGCAGCGG
    GACTCTCTGG ACTTGCCGGA GAACCAGCTG TCAACCCAGC CACTCATCAA AGTAGAGCGA
    CATCCCTCTC ACTGTGCCGA GTATCTGTCC CTCCCACACC CCGCCCCAAT GAGCGGTCTG
    GCAGGCGTTT TGTGTGAAAG CACGCAGCCG CCCCCCTTTG ATAGCAGATA TGAAGAATCC
    ACGGACTTCG GCTCTGTAAA CAGGACAGCT CAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297959.1
    CDS484..7059
    Translation

    Target ORF information:

    RefSeq Version XM_009299684.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, T type, alpha 1H subunit a (cacna1ha), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299684.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGCTGGTGA TTCTGCTGAA CTGTGTGACT CTGGGCATGT ATCAGCCATG TGAGGACCTC 
    AAGTGCCAGT CTGAGTGGTG TATCGTCCTG CAGGCGTTTG ATGACTGCAT CTTCGCCTTC
    TTCGCTGTGG AGATGGTCAT CAAAATGATC GCCCTGGGGA TTTTTGGAAT AAATGGTTAT
    CTTGGCGACA CGTGGAACCG CCTGGACTTC TTCATTGTCA TGGCAGGGAT GATGGAGTAT
    TCACTGGACG GTCATAACGC CAGCCTGTCA GCCATCCGGA CTGTGCGAGT GCTCAGGCCC
    CTGCGAGCCA TCAACAGAGT GCCCAGTATG AGGATACTGG TCACTCTGCT TCTAGATACT
    CTGCCTATGT TAGGCAACGT CCTCCTGCTG TGCTTTTTTG TCTTCTTTAT ATTCGGAATT
    GTCGGGGTGC AGCTGTGGGC TGGACTTCTT AGGAATCGGT GTTTCATGCC AAGCAAAGTG
    AGAGACCAAT ATAATCTGTC GTATATGAGT CTGTACTATG AGAACGAAGA TGGTGAAGAC
    AACCCCTTCA TCTGCTCCTC CAGTAAGGAC AACGGCATGA AGCGCTGCAG TGCTGTGCCA
    CCCCTTAAAG AGGGTGTTGA GTGCACTTTA AATGCATCTT TTCTGGGACA TGTCTACTCT
    GGCCTGAGCG GGACAGGCAA CTACAGCTGT GTGAACTGGA ACCAGTATTA CAGTGAATGC
    AAGCCCGGAG ACTTAAACCC GCACAAAGGC GCAGTAAACT TTGATAACAT TGGATATGCC
    TGGATCGCTA TATTCCAGGT TATTACATTG GAAGGATGGG TGGACATCAT GTATTACGTA
    ATGGATGCCC ACTCCTTTTA CAATTTTATA TATTTTATAT TGCTTATTAT AGTGGGTTCC
    TTCTTCATGA TCAACCTGTG CCTGGTTGTA ATAGCAACCC AGTTCGCAGA AACCAAACAG
    CGCGAGAACA TGCTGATGAA GGAGCAGCGT GCACGATACC GCTCCAACGA CAGCACCCTC
    GCCAGCTACT CTGAGCCGGG CAGCTGCTAC GAAGAGATGC TCAAGTACGT CAGCCACCTG
    TACCGCAAGG TGAAGCGCCG CCTCTCCCGC ATCTACAACA GCTGGCAGAG CAAGCGGCGA
    AAGAAAGTGA ACCCCAACAC CGGGGCTGGC GGAGGTGCCA ATGGTCACAG TAGGCACAGG
    GGTTACGGAC ACTGGGTACA GTCCATTCAC AGCCTCATCC AGCAGCACCA GCGACAGCAC
    TGTCACCTCA GCAATGGCAG CCCCAGTCCT GTGGCCACTA CGGGCTCCAG TGAGGCTCTG
    GAAATGAGGT CCATCACAGC GGGTCAGCAG CTGACCGTGT CCTCTCAGCC AACCGCCACG
    CACAATCTTT CTTCCAGCAC ACAATCAGTG CATAGCATTT ATCAAGGTGA CTTTCGTGAG
    GCCCCGCAGG AGCGAACACC CACTAATGTT GCTGTTCCGG CACTCAGCCG ACTCAATGGG
    GGGATGAACT ATCCCACAAT TTTGCCGTCC CTCATTTGCA ACTATTCAGG TGGAGGTTCA
    TTAGGGAAAG AGAGGGGGAA GTCGGATGCA AATACAGGAC ATGAGGTGAT ACAGGACTTT
    GACAAGCTGC ACCAGCAAAT TGAAGCACAT AGCCACAGTC GTGTGCTGCT GCAGATGGCT
    GGTGTGGTGC CCACTCAATT ACTTCTTGAG GTCATGAGCT GCCCGTATTG CGTAAGGGCC
    CTTGAGAGCA TGGATCTGGA GATTGGTGAT TCAGACTACC CTCGATCAGA AGGGGACATG
    GTGTATGAGT TCTCATATGA TGCCGGGTTC ATAACCGGCC GTTCAGCACA TCATAAAATG
    CAGGAGAAAC AAAACCGTCT GGTTCAATTC TGGGAGGACT TCAGGGAAAG ACTGACCCGT
    ATAGTGGATA GCAAATACTT TAACCGGGGA ATTATGATCG CCATCCTCAT CAACACCTTG
    AGTATGGGGA TTGAATACCA TGAGCAGCCC GAGGAGCTGA CCAACATCCT GGAGATCAGC
    AACATCGTCT TCACTAGCAT GTTTGTCCTC GAAATGCTCT TCAAACTGCT GGCCTTTGGC
    ATCTTCGGCT ATATAAGGAA CCCATATAAC ATCTTTGATG GGGTTATTGT TGTCATAAGT
    GTATGGGAGA TCATCGGTCA TGCGGATGGT GGTCTCTCAG TGCTGAGGAC TTTTCGTCTC
    CTGAGGGTGC TCAAACTGGT GCGCTTCCTC CCTGCCCTGC GCAGACAGCT GCTTGTCCTG
    ATGAAGACCA TGGACAATGT GGCAACATTT TGCATGCTAC TCATGCTCTT CATCTTCACT
    TTCAGCATAC TCGGAATGCA TCTGTTCGGA TGCAAATTCA GCCTGAAGAT GGAGAATGGA
    GACACTATTC CGGACAGGAA AAACTTTGAT TCCCTGCTGT GGGCCATTGT CACTGTGTTT
    CAGATTCTGA CCCAGGAAGA CTGGAACGTG GTCCTCTATA ATGGAATGGC TTCCACCAGC
    CCATGGGCGG CTCTTTATTT TGTTGCCCTC ATGACGTTTG GGAATTATGT GCTCTTTAAT
    CTACTGGTGG CCATTTTAGT GGAAGGGTTT CAGGCGGAGG GAGATGCTAA CAAGTCAGAC
    GGAGATGAGG AAAAGACATC AGTGAACTCT GAAGAGAAAA TGGAGCAGCT GCAAAGTTCA
    GAACCCCTTT CAATTTCTGA TCCAGACTTG AAGTTCTACT CTCTGATGTT GTCAGCCAAT
    GGGCATGTGG ATCCTTGTGG CACTTTGCCT CCACCAATCA TCATGCGCAC TGCTGCCACA
    CCCATGCCCA CTCCAAAGAG CTCTCTGGGG CCCGAGTCTG TGTTTGACTT GACCGAGTCC
    AGACGGGGCA GTGCTGTCTC CATAGATCCA AATGCTTTTG ATCAGAAGTC CCTGTCCAGT
    CCAAGAAGAT CACCATGCCA CCCCCGTGGC TCGGGCAGTA ACTGGGGCAG CCGCAGGTCG
    TCCTGGAACA GCTTAGGACG AGCGCCAAGT CTGAAAAGGA AGGACACATC TGGGGAGCGC
    GAGTCTCTGC TGTCCGGGGA AGGCAGGGGC AGCTTTGAGG ATGACGACAT AGAGGCGGAG
    AAGCTGACTA ACATCAGCGG AGGCTCCCTG CAGCACCCCA GCTCTCTGGA CATCCCAGAA
    CTGCCTCAAA TGGGGGAATA CCTCGACTGC AATGGACGAA GCCAACACAT ACTCGCTGAC
    CTGTCTGCCA GTCTCAACAA GGAGGACTCC ATCGCTGAGG AGGACTTGGA TGATAGCTTC
    TGTTTCCGTC TCAGGAGGAC CCTGGCAGCT TATAAACCCA AGTGGTGCAA AGATCATGAG
    GAATGGTCAC TTTACCTGTT CTCTCCTCAT AACAAGTTTC GGATGATGTG TCAAAAACTC
    ATCTCTCACA AGATGTTTGA TTATGTAGTT TTAGTATTCA TCTTCCTAAA CTGCATCACT
    ATTGCTCTGG AGAGACCCCA TATTCAACAA TCTGAGCGAT TATTTCTCCT CGTCTCAAAC
    TATGTTTTCA CTGTGATATT TGTTGCTGAA ATGACAGTGA AGGTTGTGGC CCTTGGTTTC
    TACTCCGGGA ACCAGAGTTA TCTAAAGAGC ACATGGAATG TTTTGGATGG GGTGCTGGTC
    TTTGTGTCCC TCATTGACAT TCTGGTCTCT CTGGCCTGGA CTGGAAACCG AATCTTTGGC
    ATCCTGAGGG TTCTTCGCCT GCTGCGAACT CTGCGGCCAC TGAGAGTAAT TAGTCGTGCT
    CCTGGTCTGA AGCTGGTAGT AGAAACGTTG ATAACGTCCC TGAGGCCCAT TGGGAACATT
    GTGCTGATCT GCTGTGCTTT CTTTATTGTT TTCGGCATTC TTGGAGTTCA GCTCTTCAAA
    GGAAAGTTTT TCCACTGTGA AGGCGGCGAT ACCAGAAACA TCACCAATAA GTCTGACTGT
    CTTCAGGCAA ACCTCAAATG GATCAGAAGA AAGTACAATT TTGACAACTT GGGACAGGCT
    CTCATGTCTC TTTTCGTGCT TTCCTGTAAG GATGGATGGG TCAATATAAT GTATGATGGG
    TTGGATGCTG TTGGTGTGGA CCAGCAGCCT GAGCGCAACC ACAACCCCTG GATGCTGTTA
    TATTTTATTT CCTTTCTGCT AATCGTCAGC TTCTTCGTCC TGAATATGTT TGTCGGAGTA
    GTGGTGGAAA ACTTCCACAA GTGCCGTCAA GACCAGGAGG AAGTGGAGGC GCGTCTGCTG
    GAGTTGAAGA GGCAAAAACT GATGGAGAAA AAGCGCAGGA AGGCACTCCG GAGACCATAT
    TATGCTGACT ATTCTCCAGC CCGGCTTTAC ATCCACACAC TGTGCACCAA CCACTATTTG
    GACCTGTTTA TAACCGGCAT CATCTGCATC AACGTAGTTA CCATGTCCAT CGAGCATTTC
    AATCAGCCTT CTTATCTTGA CGAGGCACTT AAATACTGTA ACTATGTATT TACAATCATC
    TTCATAATTG AGGCTCTGCT TAAACTCGTG GCATTTGGAA TACGGAGATT CTTCAAAGAC
    AGATGGAACC AGCTGGATCT TGCAATAGTC CTACTCTCTA TCATGGGCAT CACTCTGGAG
    GAGATTAAGA TGAATGCAGC GCTGCCCATC AACCCCACCA TCATCCGTAT TATGAGAGTG
    CTAAGAATCG CAAGAGTGCT GAAGCTGTTA AAGATGGCTA CAGGCATGAG ATCCCTACTC
    GACACCGTAA TGCAAGCTTT ACCACAGGTG GGAAATCTGG GCTTGCTGTT TATGCTTCTC
    TTCTTCATTT ATGCGGCGCT TGGCGTGGAG CTTTTTGGGA AACTAGAATG CACAGAGAAC
    AATCCGTGTG AAGGATTAAG TCCACATGCC ACGTTTGAGA ACTTTGGAAT GGCATTTTTG
    ACACTTTTCC GAGTATCAAC TGGGGACAAT TGGAATGGAA TAATGAAGGA CACTCTGCGA
    GAGTGCCTTC CTTCAGAGAC GCAATGCCTC AGCTATCTAC CCTGGGTCTC TCCAATCTAT
    TTTGTAACCT TTGTTCTAAT GGCTCAGTTT GTGCTGGTTA ACGTGGTCGT AGCTGTGTTG
    ATGAAACATC TGGAGGAGAG CAATAAAGAA GCTAAAGAGG ATGCAGAAAT GGATGCTGCG
    ATCAAGTTGG CAATTTTGAA AGAAACTCGC CGTCTCAGCA CCATCTCAGC CAGTGCCACT
    GCGGGACCAG AAGGCAGGGC AACTTATATC GAGCCACACC CAGATTCACC TGAGCAGGAT
    GAGGGGATGC AAAGAACACA GGGGAATTTG CTCTCTCCGC GGAAAATGTC AGTGTCAAGG
    ATGCACTCTT TACCCAATGA CAGCTACATG TTTCGCCCTG TGAGGCCAGC CAGTGCTCCG
    TATCCTCTAG AAGAAGTGGA GACCTGTCAA GATTATCTTG AATTAGGTTC CATCACCTCG
    GTTCACTCCC AGCCATGCGA AAGCCTTTCC CTGCTCCAGG TCCCTGGTGT TCCTCCCCAT
    TCCCAGTTCA ACTGCCTCCC CAAGATCCCT CCTCCCATGC CCTCTCCAAC CCACAGCATA
    GACAGGATTT TACGCAGACA GAGGGCAATT AAAAATGACT CCATTGACAT GCATAATTTC
    GACTCCAAAG AAAATGTGGA AAGACAGAAA GGGATAACCA CAGGCAGCTT CAAGCTGCAT
    GTTCCTCAAA TCACCGGCAT CTCTCAGGGG TCTCCCTTGC TGCCACCACG TGGACCTAGT
    GTCCACACCT TCCAGCAGCC GCACAGCCAG CACAATATCT CTTCTCGACC TCCCAGTCTG
    GCCAGCAGCA GCAGAAGCAC CAGCCCCAGT GGCTCAATGT TTGACTCCGC TGACCCAACC
    GATGAGGAGG TGCGCCACAT CAATAGCACA GCCCGCCTGT GGATCGGGGT TCATGCAGAA
    GCAAGGAGTC ACTCCCTCTC TCCTTACAGC ACCTCAGGGA CGCTGCTTCC CATCCCCGTG
    AAAAACTGCA GCAGTGCTGC CAACTTAACC ATCAAAGACC CCAAGAAGTG CTTCAGTGTG
    GACACTGAGG GTTTTCTGGA AAGACCCTAC GCTTCTGATG ACCATCGCAG GCACTCGATT
    GAGATTTGCT CCTCAGGAGA CGATGGGCTG TTTGGTCAAG AACTCTGTGA AAAGAGGCAC
    GTTCCCACGC GTGGAAAGTC TTTGCACATT CCCCGGAAGA CAAAGATGAG CCCTCCCTGC
    ATCTCCATCG AGCCCCCATT TGAAAAGGAT GTTTCCCCCT CTCATGCGTC CATGAAAAAG
    TTGTCGGATA GCATGTTACT GCGGAGGAGG ACACCATCCT ATGACCTGGC CCTGCAGCGG
    GACTCTCTGG ACTTGCCGGA GAACCAGCTG TCAACCCAGC CACTCATCAA AGTAGAGCGA
    CATCCCTCTC ACTGTGCCGA GTATCTGTCC CTCCCACACC CCGCCCCAAT GAGCGGTCTG
    GCAGGCGTTT TGTGTGAAAG CACGCAGCCG CCCCCCTTTG ATAGCAGATA TGAAGAATCC
    ACGGACTTCG GCTCTGTAAA CAGGACAGCT CAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43366
    Clone ID Related Accession (Same CDS sequence) XM_009299684.3 , XM_009299684.2
    Accession Version XM_009299684.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent T-type calcium channel subunit alpha-1H isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299684.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGCTGGTGA TTCTGCTGAA CTGTGTGACT CTGGGCATGT ATCAGCCATG TGAGGACCTC 
    AAGTGCCAGT CTGAGTGGTG TATCGTCCTG CAGGCGTTTG ATGACTGCAT CTTCGCCTTC
    TTCGCTGTGG AGATGGTCAT CAAAATGATC GCCCTGGGGA TTTTTGGAAT AAATGGTTAT
    CTTGGCGACA CGTGGAACCG CCTGGACTTC TTCATTGTCA TGGCAGGGAT GATGGAGTAT
    TCACTGGACG GTCATAACGC CAGCCTGTCA GCCATCCGGA CTGTGCGAGT GCTCAGGCCC
    CTGCGAGCCA TCAACAGAGT GCCCAGTATG AGGATACTGG TCACTCTGCT TCTAGATACT
    CTGCCTATGT TAGGCAACGT CCTCCTGCTG TGCTTTTTTG TCTTCTTTAT ATTCGGAATT
    GTCGGGGTGC AGCTGTGGGC TGGACTTCTT AGGAATCGGT GTTTCATGCC AAGCAAAGTG
    AGAGACCAAT ATAATCTGTC GTATATGAGT CTGTACTATG AGAACGAAGA TGGTGAAGAC
    AACCCCTTCA TCTGCTCCTC CAGTAAGGAC AACGGCATGA AGCGCTGCAG TGCTGTGCCA
    CCCCTTAAAG AGGGTGTTGA GTGCACTTTA AATGCATCTT TTCTGGGACA TGTCTACTCT
    GGCCTGAGCG GGACAGGCAA CTACAGCTGT GTGAACTGGA ACCAGTATTA CAGTGAATGC
    AAGCCCGGAG ACTTAAACCC GCACAAAGGC GCAGTAAACT TTGATAACAT TGGATATGCC
    TGGATCGCTA TATTCCAGGT TATTACATTG GAAGGATGGG TGGACATCAT GTATTACGTA
    ATGGATGCCC ACTCCTTTTA CAATTTTATA TATTTTATAT TGCTTATTAT AGTGGGTTCC
    TTCTTCATGA TCAACCTGTG CCTGGTTGTA ATAGCAACCC AGTTCGCAGA AACCAAACAG
    CGCGAGAACA TGCTGATGAA GGAGCAGCGT GCACGATACC GCTCCAACGA CAGCACCCTC
    GCCAGCTACT CTGAGCCGGG CAGCTGCTAC GAAGAGATGC TCAAGTACGT CAGCCACCTG
    TACCGCAAGG TGAAGCGCCG CCTCTCCCGC ATCTACAACA GCTGGCAGAG CAAGCGGCGA
    AAGAAAGTGA ACCCCAACAC CGGGGCTGGC GGAGGTGCCA ATGGTCACAG TAGGCACAGG
    GGTTACGGAC ACTGGGTACA GTCCATTCAC AGCCTCATCC AGCAGCACCA GCGACAGCAC
    TGTCACCTCA GCAATGGCAG CCCCAGTCCT GTGGCCACTA CGGGCTCCAG TGAGGCTCTG
    GAAATGAGGT CCATCACAGC GGGTCAGCAG CTGACCGTGT CCTCTCAGCC AACCGCCACG
    CACAATCTTT CTTCCAGCAC ACAATCAGTG CATAGCATTT ATCAAGGTGA CTTTCGTGAG
    GCCCCGCAGG AGCGAACACC CACTAATGTT GCTGTTCCGG CACTCAGCCG ACTCAATGGG
    GGGATGAACT ATCCCACAAT TTTGCCGTCC CTCATTTGCA ACTATTCAGG TGGAGGTTCA
    TTAGGGAAAG AGAGGGGGAA GTCGGATGCA AATACAGGAC ATGAGGTGAT ACAGGACTTT
    GACAAGCTGC ACCAGCAAAT TGAAGCACAT AGCCACAGTC GTGTGCTGCT GCAGATGGCT
    GGTGTGGTGC CCACTCAATT ACTTCTTGAG GTCATGAGCT GCCCGTATTG CGTAAGGGCC
    CTTGAGAGCA TGGATCTGGA GATTGGTGAT TCAGACTACC CTCGATCAGA AGGGGACATG
    GTGTATGAGT TCTCATATGA TGCCGGGTTC ATAACCGGCC GTTCAGCACA TCATAAAATG
    CAGGAGAAAC AAAACCGTCT GGTTCAATTC TGGGAGGACT TCAGGGAAAG ACTGACCCGT
    ATAGTGGATA GCAAATACTT TAACCGGGGA ATTATGATCG CCATCCTCAT CAACACCTTG
    AGTATGGGGA TTGAATACCA TGAGCAGCCC GAGGAGCTGA CCAACATCCT GGAGATCAGC
    AACATCGTCT TCACTAGCAT GTTTGTCCTC GAAATGCTCT TCAAACTGCT GGCCTTTGGC
    ATCTTCGGCT ATATAAGGAA CCCATATAAC ATCTTTGATG GGGTTATTGT TGTCATAAGT
    GTATGGGAGA TCATCGGTCA TGCGGATGGT GGTCTCTCAG TGCTGAGGAC TTTTCGTCTC
    CTGAGGGTGC TCAAACTGGT GCGCTTCCTC CCTGCCCTGC GCAGACAGCT GCTTGTCCTG
    ATGAAGACCA TGGACAATGT GGCAACATTT TGCATGCTAC TCATGCTCTT CATCTTCACT
    TTCAGCATAC TCGGAATGCA TCTGTTCGGA TGCAAATTCA GCCTGAAGAT GGAGAATGGA
    GACACTATTC CGGACAGGAA AAACTTTGAT TCCCTGCTGT GGGCCATTGT CACTGTGTTT
    CAGATTCTGA CCCAGGAAGA CTGGAACGTG GTCCTCTATA ATGGAATGGC TTCCACCAGC
    CCATGGGCGG CTCTTTATTT TGTTGCCCTC ATGACGTTTG GGAATTATGT GCTCTTTAAT
    CTACTGGTGG CCATTTTAGT GGAAGGGTTT CAGGCGGAGG GAGATGCTAA CAAGTCAGAC
    GGAGATGAGG AAAAGACATC AGTGAACTCT GAAGAGAAAA TGGAGCAGCT GCAAAGTTCA
    GAACCCCTTT CAATTTCTGA TCCAGACTTG AAGTTCTACT CTCTGATGTT GTCAGCCAAT
    GGGCATGTGG ATCCTTGTGG CACTTTGCCT CCACCAATCA TCATGCGCAC TGCTGCCACA
    CCCATGCCCA CTCCAAAGAG CTCTCTGGGG CCCGAGTCTG TGTTTGACTT GACCGAGTCC
    AGACGGGGCA GTGCTGTCTC CATAGATCCA AATGCTTTTG ATCAGAAGTC CCTGTCCAGT
    CCAAGAAGAT CACCATGCCA CCCCCGTGGC TCGGGCAGTA ACTGGGGCAG CCGCAGGTCG
    TCCTGGAACA GCTTAGGACG AGCGCCAAGT CTGAAAAGGA AGGACACATC TGGGGAGCGC
    GAGTCTCTGC TGTCCGGGGA AGGCAGGGGC AGCTTTGAGG ATGACGACAT AGAGGCGGAG
    AAGCTGACTA ACATCAGCGG AGGCTCCCTG CAGCACCCCA GCTCTCTGGA CATCCCAGAA
    CTGCCTCAAA TGGGGGAATA CCTCGACTGC AATGGACGAA GCCAACACAT ACTCGCTGAC
    CTGTCTGCCA GTCTCAACAA GGAGGACTCC ATCGCTGAGG AGGACTTGGA TGATAGCTTC
    TGTTTCCGTC TCAGGAGGAC CCTGGCAGCT TATAAACCCA AGTGGTGCAA AGATCATGAG
    GAATGGTCAC TTTACCTGTT CTCTCCTCAT AACAAGTTTC GGATGATGTG TCAAAAACTC
    ATCTCTCACA AGATGTTTGA TTATGTAGTT TTAGTATTCA TCTTCCTAAA CTGCATCACT
    ATTGCTCTGG AGAGACCCCA TATTCAACAA TCTGAGCGAT TATTTCTCCT CGTCTCAAAC
    TATGTTTTCA CTGTGATATT TGTTGCTGAA ATGACAGTGA AGGTTGTGGC CCTTGGTTTC
    TACTCCGGGA ACCAGAGTTA TCTAAAGAGC ACATGGAATG TTTTGGATGG GGTGCTGGTC
    TTTGTGTCCC TCATTGACAT TCTGGTCTCT CTGGCCTGGA CTGGAAACCG AATCTTTGGC
    ATCCTGAGGG TTCTTCGCCT GCTGCGAACT CTGCGGCCAC TGAGAGTAAT TAGTCGTGCT
    CCTGGTCTGA AGCTGGTAGT AGAAACGTTG ATAACGTCCC TGAGGCCCAT TGGGAACATT
    GTGCTGATCT GCTGTGCTTT CTTTATTGTT TTCGGCATTC TTGGAGTTCA GCTCTTCAAA
    GGAAAGTTTT TCCACTGTGA AGGCGGCGAT ACCAGAAACA TCACCAATAA GTCTGACTGT
    CTTCAGGCAA ACCTCAAATG GATCAGAAGA AAGTACAATT TTGACAACTT GGGACAGGCT
    CTCATGTCTC TTTTCGTGCT TTCCTGTAAG GATGGATGGG TCAATATAAT GTATGATGGG
    TTGGATGCTG TTGGTGTGGA CCAGCAGCCT GAGCGCAACC ACAACCCCTG GATGCTGTTA
    TATTTTATTT CCTTTCTGCT AATCGTCAGC TTCTTCGTCC TGAATATGTT TGTCGGAGTA
    GTGGTGGAAA ACTTCCACAA GTGCCGTCAA GACCAGGAGG AAGTGGAGGC GCGTCTGCTG
    GAGTTGAAGA GGCAAAAACT GATGGAGAAA AAGCGCAGGA AGGCACTCCG GAGACCATAT
    TATGCTGACT ATTCTCCAGC CCGGCTTTAC ATCCACACAC TGTGCACCAA CCACTATTTG
    GACCTGTTTA TAACCGGCAT CATCTGCATC AACGTAGTTA CCATGTCCAT CGAGCATTTC
    AATCAGCCTT CTTATCTTGA CGAGGCACTT AAATACTGTA ACTATGTATT TACAATCATC
    TTCATAATTG AGGCTCTGCT TAAACTCGTG GCATTTGGAA TACGGAGATT CTTCAAAGAC
    AGATGGAACC AGCTGGATCT TGCAATAGTC CTACTCTCTA TCATGGGCAT CACTCTGGAG
    GAGATTAAGA TGAATGCAGC GCTGCCCATC AACCCCACCA TCATCCGTAT TATGAGAGTG
    CTAAGAATCG CAAGAGTGCT GAAGCTGTTA AAGATGGCTA CAGGCATGAG ATCCCTACTC
    GACACCGTAA TGCAAGCTTT ACCACAGGTG GGAAATCTGG GCTTGCTGTT TATGCTTCTC
    TTCTTCATTT ATGCGGCGCT TGGCGTGGAG CTTTTTGGGA AACTAGAATG CACAGAGAAC
    AATCCGTGTG AAGGATTAAG TCCACATGCC ACGTTTGAGA ACTTTGGAAT GGCATTTTTG
    ACACTTTTCC GAGTATCAAC TGGGGACAAT TGGAATGGAA TAATGAAGGA CACTCTGCGA
    GAGTGCCTTC CTTCAGAGAC GCAATGCCTC AGCTATCTAC CCTGGGTCTC TCCAATCTAT
    TTTGTAACCT TTGTTCTAAT GGCTCAGTTT GTGCTGGTTA ACGTGGTCGT AGCTGTGTTG
    ATGAAACATC TGGAGGAGAG CAATAAAGAA GCTAAAGAGG ATGCAGAAAT GGATGCTGCG
    ATCAAGTTGG CAATTTTGAA AGAAACTCGC CGTCTCAGCA CCATCTCAGC CAGTGCCACT
    GCGGGACCAG AAGGCAGGGC AACTTATATC GAGCCACACC CAGATTCACC TGAGCAGGAT
    GAGGGGATGC AAAGAACACA GGGGAATTTG CTCTCTCCGC GGAAAATGTC AGTGTCAAGG
    ATGCACTCTT TACCCAATGA CAGCTACATG TTTCGCCCTG TGAGGCCAGC CAGTGCTCCG
    TATCCTCTAG AAGAAGTGGA GACCTGTCAA GATTATCTTG AATTAGGTTC CATCACCTCG
    GTTCACTCCC AGCCATGCGA AAGCCTTTCC CTGCTCCAGG TCCCTGGTGT TCCTCCCCAT
    TCCCAGTTCA ACTGCCTCCC CAAGATCCCT CCTCCCATGC CCTCTCCAAC CCACAGCATA
    GACAGGATTT TACGCAGACA GAGGGCAATT AAAAATGACT CCATTGACAT GCATAATTTC
    GACTCCAAAG AAAATGTGGA AAGACAGAAA GGGATAACCA CAGGCAGCTT CAAGCTGCAT
    GTTCCTCAAA TCACCGGCAT CTCTCAGGGG TCTCCCTTGC TGCCACCACG TGGACCTAGT
    GTCCACACCT TCCAGCAGCC GCACAGCCAG CACAATATCT CTTCTCGACC TCCCAGTCTG
    GCCAGCAGCA GCAGAAGCAC CAGCCCCAGT GGCTCAATGT TTGACTCCGC TGACCCAACC
    GATGAGGAGG TGCGCCACAT CAATAGCACA GCCCGCCTGT GGATCGGGGT TCATGCAGAA
    GCAAGGAGTC ACTCCCTCTC TCCTTACAGC ACCTCAGGGA CGCTGCTTCC CATCCCCGTG
    AAAAACTGCA GCAGTGCTGC CAACTTAACC ATCAAAGACC CCAAGAAGTG CTTCAGTGTG
    GACACTGAGG GTTTTCTGGA AAGACCCTAC GCTTCTGATG ACCATCGCAG GCACTCGATT
    GAGATTTGCT CCTCAGGAGA CGATGGGCTG TTTGGTCAAG AACTCTGTGA AAAGAGGCAC
    GTTCCCACGC GTGGAAAGTC TTTGCACATT CCCCGGAAGA CAAAGATGAG CCCTCCCTGC
    ATCTCCATCG AGCCCCCATT TGAAAAGGAT GTTTCCCCCT CTCATGCGTC CATGAAAAAG
    TTGTCGGATA GCATGTTACT GCGGAGGAGG ACACCATCCT ATGACCTGGC CCTGCAGCGG
    GACTCTCTGG ACTTGCCGGA GAACCAGCTG TCAACCCAGC CACTCATCAA AGTAGAGCGA
    CATCCCTCTC ACTGTGCCGA GTATCTGTCC CTCCCACACC CCGCCCCAAT GAGCGGTCTG
    GCAGGCGTTT TGTGTGAAAG CACGCAGCCG CCCCCCTTTG ATAGCAGATA TGAAGAATCC
    ACGGACTTCG GCTCTGTAAA CAGGACAGCT CAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297959.1
    CDS19..6594
    Translation

    Target ORF information:

    RefSeq Version XM_009299684.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, T type, alpha 1H subunit a (cacna1ha), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299684.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGCTGGTGA TTCTGCTGAA CTGTGTGACT CTGGGCATGT ATCAGCCATG TGAGGACCTC 
    AAGTGCCAGT CTGAGTGGTG TATCGTCCTG CAGGCGTTTG ATGACTGCAT CTTCGCCTTC
    TTCGCTGTGG AGATGGTCAT CAAAATGATC GCCCTGGGGA TTTTTGGAAT AAATGGTTAT
    CTTGGCGACA CGTGGAACCG CCTGGACTTC TTCATTGTCA TGGCAGGGAT GATGGAGTAT
    TCACTGGACG GTCATAACGC CAGCCTGTCA GCCATCCGGA CTGTGCGAGT GCTCAGGCCC
    CTGCGAGCCA TCAACAGAGT GCCCAGTATG AGGATACTGG TCACTCTGCT TCTAGATACT
    CTGCCTATGT TAGGCAACGT CCTCCTGCTG TGCTTTTTTG TCTTCTTTAT ATTCGGAATT
    GTCGGGGTGC AGCTGTGGGC TGGACTTCTT AGGAATCGGT GTTTCATGCC AAGCAAAGTG
    AGAGACCAAT ATAATCTGTC GTATATGAGT CTGTACTATG AGAACGAAGA TGGTGAAGAC
    AACCCCTTCA TCTGCTCCTC CAGTAAGGAC AACGGCATGA AGCGCTGCAG TGCTGTGCCA
    CCCCTTAAAG AGGGTGTTGA GTGCACTTTA AATGCATCTT TTCTGGGACA TGTCTACTCT
    GGCCTGAGCG GGACAGGCAA CTACAGCTGT GTGAACTGGA ACCAGTATTA CAGTGAATGC
    AAGCCCGGAG ACTTAAACCC GCACAAAGGC GCAGTAAACT TTGATAACAT TGGATATGCC
    TGGATCGCTA TATTCCAGGT TATTACATTG GAAGGATGGG TGGACATCAT GTATTACGTA
    ATGGATGCCC ACTCCTTTTA CAATTTTATA TATTTTATAT TGCTTATTAT AGTGGGTTCC
    TTCTTCATGA TCAACCTGTG CCTGGTTGTA ATAGCAACCC AGTTCGCAGA AACCAAACAG
    CGCGAGAACA TGCTGATGAA GGAGCAGCGT GCACGATACC GCTCCAACGA CAGCACCCTC
    GCCAGCTACT CTGAGCCGGG CAGCTGCTAC GAAGAGATGC TCAAGTACGT CAGCCACCTG
    TACCGCAAGG TGAAGCGCCG CCTCTCCCGC ATCTACAACA GCTGGCAGAG CAAGCGGCGA
    AAGAAAGTGA ACCCCAACAC CGGGGCTGGC GGAGGTGCCA ATGGTCACAG TAGGCACAGG
    GGTTACGGAC ACTGGGTACA GTCCATTCAC AGCCTCATCC AGCAGCACCA GCGACAGCAC
    TGTCACCTCA GCAATGGCAG CCCCAGTCCT GTGGCCACTA CGGGCTCCAG TGAGGCTCTG
    GAAATGAGGT CCATCACAGC GGGTCAGCAG CTGACCGTGT CCTCTCAGCC AACCGCCACG
    CACAATCTTT CTTCCAGCAC ACAATCAGTG CATAGCATTT ATCAAGGTGA CTTTCGTGAG
    GCCCCGCAGG AGCGAACACC CACTAATGTT GCTGTTCCGG CACTCAGCCG ACTCAATGGG
    GGGATGAACT ATCCCACAAT TTTGCCGTCC CTCATTTGCA ACTATTCAGG TGGAGGTTCA
    TTAGGGAAAG AGAGGGGGAA GTCGGATGCA AATACAGGAC ATGAGGTGAT ACAGGACTTT
    GACAAGCTGC ACCAGCAAAT TGAAGCACAT AGCCACAGTC GTGTGCTGCT GCAGATGGCT
    GGTGTGGTGC CCACTCAATT ACTTCTTGAG GTCATGAGCT GCCCGTATTG CGTAAGGGCC
    CTTGAGAGCA TGGATCTGGA GATTGGTGAT TCAGACTACC CTCGATCAGA AGGGGACATG
    GTGTATGAGT TCTCATATGA TGCCGGGTTC ATAACCGGCC GTTCAGCACA TCATAAAATG
    CAGGAGAAAC AAAACCGTCT GGTTCAATTC TGGGAGGACT TCAGGGAAAG ACTGACCCGT
    ATAGTGGATA GCAAATACTT TAACCGGGGA ATTATGATCG CCATCCTCAT CAACACCTTG
    AGTATGGGGA TTGAATACCA TGAGCAGCCC GAGGAGCTGA CCAACATCCT GGAGATCAGC
    AACATCGTCT TCACTAGCAT GTTTGTCCTC GAAATGCTCT TCAAACTGCT GGCCTTTGGC
    ATCTTCGGCT ATATAAGGAA CCCATATAAC ATCTTTGATG GGGTTATTGT TGTCATAAGT
    GTATGGGAGA TCATCGGTCA TGCGGATGGT GGTCTCTCAG TGCTGAGGAC TTTTCGTCTC
    CTGAGGGTGC TCAAACTGGT GCGCTTCCTC CCTGCCCTGC GCAGACAGCT GCTTGTCCTG
    ATGAAGACCA TGGACAATGT GGCAACATTT TGCATGCTAC TCATGCTCTT CATCTTCACT
    TTCAGCATAC TCGGAATGCA TCTGTTCGGA TGCAAATTCA GCCTGAAGAT GGAGAATGGA
    GACACTATTC CGGACAGGAA AAACTTTGAT TCCCTGCTGT GGGCCATTGT CACTGTGTTT
    CAGATTCTGA CCCAGGAAGA CTGGAACGTG GTCCTCTATA ATGGAATGGC TTCCACCAGC
    CCATGGGCGG CTCTTTATTT TGTTGCCCTC ATGACGTTTG GGAATTATGT GCTCTTTAAT
    CTACTGGTGG CCATTTTAGT GGAAGGGTTT CAGGCGGAGG GAGATGCTAA CAAGTCAGAC
    GGAGATGAGG AAAAGACATC AGTGAACTCT GAAGAGAAAA TGGAGCAGCT GCAAAGTTCA
    GAACCCCTTT CAATTTCTGA TCCAGACTTG AAGTTCTACT CTCTGATGTT GTCAGCCAAT
    GGGCATGTGG ATCCTTGTGG CACTTTGCCT CCACCAATCA TCATGCGCAC TGCTGCCACA
    CCCATGCCCA CTCCAAAGAG CTCTCTGGGG CCCGAGTCTG TGTTTGACTT GACCGAGTCC
    AGACGGGGCA GTGCTGTCTC CATAGATCCA AATGCTTTTG ATCAGAAGTC CCTGTCCAGT
    CCAAGAAGAT CACCATGCCA CCCCCGTGGC TCGGGCAGTA ACTGGGGCAG CCGCAGGTCG
    TCCTGGAACA GCTTAGGACG AGCGCCAAGT CTGAAAAGGA AGGACACATC TGGGGAGCGC
    GAGTCTCTGC TGTCCGGGGA AGGCAGGGGC AGCTTTGAGG ATGACGACAT AGAGGCGGAG
    AAGCTGACTA ACATCAGCGG AGGCTCCCTG CAGCACCCCA GCTCTCTGGA CATCCCAGAA
    CTGCCTCAAA TGGGGGAATA CCTCGACTGC AATGGACGAA GCCAACACAT ACTCGCTGAC
    CTGTCTGCCA GTCTCAACAA GGAGGACTCC ATCGCTGAGG AGGACTTGGA TGATAGCTTC
    TGTTTCCGTC TCAGGAGGAC CCTGGCAGCT TATAAACCCA AGTGGTGCAA AGATCATGAG
    GAATGGTCAC TTTACCTGTT CTCTCCTCAT AACAAGTTTC GGATGATGTG TCAAAAACTC
    ATCTCTCACA AGATGTTTGA TTATGTAGTT TTAGTATTCA TCTTCCTAAA CTGCATCACT
    ATTGCTCTGG AGAGACCCCA TATTCAACAA TCTGAGCGAT TATTTCTCCT CGTCTCAAAC
    TATGTTTTCA CTGTGATATT TGTTGCTGAA ATGACAGTGA AGGTTGTGGC CCTTGGTTTC
    TACTCCGGGA ACCAGAGTTA TCTAAAGAGC ACATGGAATG TTTTGGATGG GGTGCTGGTC
    TTTGTGTCCC TCATTGACAT TCTGGTCTCT CTGGCCTGGA CTGGAAACCG AATCTTTGGC
    ATCCTGAGGG TTCTTCGCCT GCTGCGAACT CTGCGGCCAC TGAGAGTAAT TAGTCGTGCT
    CCTGGTCTGA AGCTGGTAGT AGAAACGTTG ATAACGTCCC TGAGGCCCAT TGGGAACATT
    GTGCTGATCT GCTGTGCTTT CTTTATTGTT TTCGGCATTC TTGGAGTTCA GCTCTTCAAA
    GGAAAGTTTT TCCACTGTGA AGGCGGCGAT ACCAGAAACA TCACCAATAA GTCTGACTGT
    CTTCAGGCAA ACCTCAAATG GATCAGAAGA AAGTACAATT TTGACAACTT GGGACAGGCT
    CTCATGTCTC TTTTCGTGCT TTCCTGTAAG GATGGATGGG TCAATATAAT GTATGATGGG
    TTGGATGCTG TTGGTGTGGA CCAGCAGCCT GAGCGCAACC ACAACCCCTG GATGCTGTTA
    TATTTTATTT CCTTTCTGCT AATCGTCAGC TTCTTCGTCC TGAATATGTT TGTCGGAGTA
    GTGGTGGAAA ACTTCCACAA GTGCCGTCAA GACCAGGAGG AAGTGGAGGC GCGTCTGCTG
    GAGTTGAAGA GGCAAAAACT GATGGAGAAA AAGCGCAGGA AGGCACTCCG GAGACCATAT
    TATGCTGACT ATTCTCCAGC CCGGCTTTAC ATCCACACAC TGTGCACCAA CCACTATTTG
    GACCTGTTTA TAACCGGCAT CATCTGCATC AACGTAGTTA CCATGTCCAT CGAGCATTTC
    AATCAGCCTT CTTATCTTGA CGAGGCACTT AAATACTGTA ACTATGTATT TACAATCATC
    TTCATAATTG AGGCTCTGCT TAAACTCGTG GCATTTGGAA TACGGAGATT CTTCAAAGAC
    AGATGGAACC AGCTGGATCT TGCAATAGTC CTACTCTCTA TCATGGGCAT CACTCTGGAG
    GAGATTAAGA TGAATGCAGC GCTGCCCATC AACCCCACCA TCATCCGTAT TATGAGAGTG
    CTAAGAATCG CAAGAGTGCT GAAGCTGTTA AAGATGGCTA CAGGCATGAG ATCCCTACTC
    GACACCGTAA TGCAAGCTTT ACCACAGGTG GGAAATCTGG GCTTGCTGTT TATGCTTCTC
    TTCTTCATTT ATGCGGCGCT TGGCGTGGAG CTTTTTGGGA AACTAGAATG CACAGAGAAC
    AATCCGTGTG AAGGATTAAG TCCACATGCC ACGTTTGAGA ACTTTGGAAT GGCATTTTTG
    ACACTTTTCC GAGTATCAAC TGGGGACAAT TGGAATGGAA TAATGAAGGA CACTCTGCGA
    GAGTGCCTTC CTTCAGAGAC GCAATGCCTC AGCTATCTAC CCTGGGTCTC TCCAATCTAT
    TTTGTAACCT TTGTTCTAAT GGCTCAGTTT GTGCTGGTTA ACGTGGTCGT AGCTGTGTTG
    ATGAAACATC TGGAGGAGAG CAATAAAGAA GCTAAAGAGG ATGCAGAAAT GGATGCTGCG
    ATCAAGTTGG CAATTTTGAA AGAAACTCGC CGTCTCAGCA CCATCTCAGC CAGTGCCACT
    GCGGGACCAG AAGGCAGGGC AACTTATATC GAGCCACACC CAGATTCACC TGAGCAGGAT
    GAGGGGATGC AAAGAACACA GGGGAATTTG CTCTCTCCGC GGAAAATGTC AGTGTCAAGG
    ATGCACTCTT TACCCAATGA CAGCTACATG TTTCGCCCTG TGAGGCCAGC CAGTGCTCCG
    TATCCTCTAG AAGAAGTGGA GACCTGTCAA GATTATCTTG AATTAGGTTC CATCACCTCG
    GTTCACTCCC AGCCATGCGA AAGCCTTTCC CTGCTCCAGG TCCCTGGTGT TCCTCCCCAT
    TCCCAGTTCA ACTGCCTCCC CAAGATCCCT CCTCCCATGC CCTCTCCAAC CCACAGCATA
    GACAGGATTT TACGCAGACA GAGGGCAATT AAAAATGACT CCATTGACAT GCATAATTTC
    GACTCCAAAG AAAATGTGGA AAGACAGAAA GGGATAACCA CAGGCAGCTT CAAGCTGCAT
    GTTCCTCAAA TCACCGGCAT CTCTCAGGGG TCTCCCTTGC TGCCACCACG TGGACCTAGT
    GTCCACACCT TCCAGCAGCC GCACAGCCAG CACAATATCT CTTCTCGACC TCCCAGTCTG
    GCCAGCAGCA GCAGAAGCAC CAGCCCCAGT GGCTCAATGT TTGACTCCGC TGACCCAACC
    GATGAGGAGG TGCGCCACAT CAATAGCACA GCCCGCCTGT GGATCGGGGT TCATGCAGAA
    GCAAGGAGTC ACTCCCTCTC TCCTTACAGC ACCTCAGGGA CGCTGCTTCC CATCCCCGTG
    AAAAACTGCA GCAGTGCTGC CAACTTAACC ATCAAAGACC CCAAGAAGTG CTTCAGTGTG
    GACACTGAGG GTTTTCTGGA AAGACCCTAC GCTTCTGATG ACCATCGCAG GCACTCGATT
    GAGATTTGCT CCTCAGGAGA CGATGGGCTG TTTGGTCAAG AACTCTGTGA AAAGAGGCAC
    GTTCCCACGC GTGGAAAGTC TTTGCACATT CCCCGGAAGA CAAAGATGAG CCCTCCCTGC
    ATCTCCATCG AGCCCCCATT TGAAAAGGAT GTTTCCCCCT CTCATGCGTC CATGAAAAAG
    TTGTCGGATA GCATGTTACT GCGGAGGAGG ACACCATCCT ATGACCTGGC CCTGCAGCGG
    GACTCTCTGG ACTTGCCGGA GAACCAGCTG TCAACCCAGC CACTCATCAA AGTAGAGCGA
    CATCCCTCTC ACTGTGCCGA GTATCTGTCC CTCCCACACC CCGCCCCAAT GAGCGGTCTG
    GCAGGCGTTT TGTGTGAAAG CACGCAGCCG CCCCCCTTTG ATAGCAGATA TGAAGAATCC
    ACGGACTTCG GCTCTGTAAA CAGGACAGCT CAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43367
    Clone ID Related Accession (Same CDS sequence) XM_009299685.3 , XM_009299685.2
    Accession Version XM_009299685.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6573bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent T-type calcium channel subunit alpha-1H isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299685.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGCTGGTGA TTCTGCTGAA CTGTGTGACT CTGGGCATGT ATCAGCCATG TGAGGACCTC 
    AAGTGCCAGT CTGAGTGGTG TATCGTCCTG CAGGCGTTTG ATGACTGCAT CTTCGCCTTC
    TTCGCTGTGG AGATGGTCAT CAAAATGATC GCCCTGGGGA TTTTTGGAAT AAATGGTTAT
    CTTGGCGACA CGTGGAACCG CCTGGACTTC TTCATTGTCA TGGCAGGGAT GATGGAGTAT
    TCACTGGACG GTCATAACGC CAGCCTGTCA GCCATCCGGA CTGTGCGAGT GCTCAGGCCC
    CTGCGAGCCA TCAACAGAGT GCCCAGTATG AGGATACTGG TCACTCTGCT TCTAGATACT
    CTGCCTATGT TAGGCAACGT CCTCCTGCTG TGCTTTTTTG TCTTCTTTAT ATTCGGAATT
    GTCGGGGTGC AGCTGTGGGC TGGACTTCTT AGGAATCGGT GTTTCATGCC AAGCAAAGTG
    AGAGACCAAT ATAATCTGTC GTATATGAGT CTGTACTATG AGAACGAAGA TGGTGAAGAC
    AACCCCTTCA TCTGCTCCTC CAGTAAGGAC AACGGCATGA AGCGCTGCAG TGCTGTGCCA
    CCCCTTAAAG AGGGTGTTGA GTGCACTTTA AATGCATCTT TTCTGGGACA TGTCTACTCT
    GGCCTGAGCG GGACAGGCAA CTACAGCTGT GTGAACTGGA ACCAGTATTA CAGTGAATGC
    AAGCCCGGAG ACTTAAACCC GCACAAAGGC GCAGTAAACT TTGATAACAT TGGATATGCC
    TGGATCGCTA TATTCCAGGT TATTACATTG GAAGGATGGG TGGACATCAT GTATTACGTA
    ATGGATGCCC ACTCCTTTTA CAATTTTATA TATTTTATAT TGCTTATTAT AGTGGGTTCC
    TTCTTCATGA TCAACCTGTG CCTGGTTGTA ATAGCAACCC AGTTCGCAGA AACCAAACAG
    CGCGAGAACA TGCTGATGAA GGAGCAGCGT GCACGATACC GCTCCAACGA CAGCACCCTC
    GCCAGCTACT CTGAGCCGGG CAGCTGCTAC GAAGAGATGC TCAAGTACGT CAGCCACCTG
    TACCGCAAGG TGAAGCGCCG CCTCTCCCGC ATCTACAACA GCTGGCAGAG CAAGCGGCGA
    AAGAAAGTGA ACCCCAACAC CGGGGCTGGC GGAGGTGCCA ATGGTCACAG TAGGCACAGG
    GGTTACGGAC ACTGGGTACA GTCCATTCAC AGCCTCATCC AGCAGCACCA GCGACAGCAC
    TGTCACCTCA GCAATGGCAG CCCCAGTCCT GTGGCCACTA CGGGCTCCAG TGAGGCTCTG
    GAAATGAGGT CCATCACAGC GGGTCAGCAG CTGACCGTGT CCTCTCAGCC AACCGCCACG
    CACAATCTTT CTTCCAGCAC ACAATCAGTG CATAGCATTT ATCAAGGTGA CTTTCGTGAG
    GCCCCGCAGG AGCGAACACC CACTAATGTT GCTGTTCCGG CACTCAGCCG ACTCAATGGG
    GGGATGAACT ATCCCACAAT TTTGCCGTCC CTCATTTGCA ACTATTCAGG TGGAGGTTCA
    TTAGGGAAAG AGAGGGGGAA GTCGGATGCA AATACAGGAC ATGAGGTGAT ACAGGACTTT
    GACAAGCTGC ACCAGCAAAT TGAAGCACAT AGCCACAGTC GTGTGCTGCT GCAGATGGCT
    GGTGTGGTGC CCACTCAATT ACTTCTTGAG GTCATGAGCT GCCCGTATTG CGTAAGGGCC
    CTTGAGAGCA TGGATCTGGA GATTGGTGAT TCAGACTACC CTCGATCAGA AGGGGACATG
    GTGTATGAGT TCTCATATGA TGCCGGGTTC ATAACCGGCC GTTCAGCACA TCATAAAATG
    CAGGAGAAAC AAAACCGTCT GGTTCAATTC TGGGAGGACT TCAGGGAAAG ACTGACCCGT
    ATAGTGGATA GCAAATACTT TAACCGGGGA ATTATGATCG CCATCCTCAT CAACACCTTG
    AGTATGGGGA TTGAATACCA TGAGCAGCCC GAGGAGCTGA CCAACATCCT GGAGATCAGC
    AACATCGTCT TCACTAGCAT GTTTGTCCTC GAAATGCTCT TCAAACTGCT GGCCTTTGGC
    ATCTTCGGCT ATATAAGGAA CCCATATAAC ATCTTTGATG GGGTTATTGT TGTCATAAGT
    GTATGGGAGA TCATCGGTCA TGCGGATGGT GGTCTCTCAG TGCTGAGGAC TTTTCGTCTC
    CTGAGGGTGC TCAAACTGGT GCGCTTCCTC CCTGCCCTGC GCAGACAGCT GCTTGTCCTG
    ATGAAGACCA TGGACAATGT GGCAACATTT TGCATGCTAC TCATGCTCTT CATCTTCACT
    TTCAGCATAC TCGGAATGCA TCTGTTCGGA TGCAAATTCA GCCTGAAGAT GGAGAATGGA
    GACACTATTC CGGACAGGAA AAACTTTGAT TCCCTGCTGT GGGCCATTGT CACTGTGTTT
    CAGATTCTGA CCCAGGAAGA CTGGAACGTG GTCCTCTATA ATGGAATGGC TTCCACCAGC
    CCATGGGCGG CTCTTTATTT TGTTGCCCTC ATGACGTTTG GGAATTATGT GCTCTTTAAT
    CTACTGGTGG CCATTTTAGT GGAAGGGTTT CAGGCGGAGG GAGATGCTAA CAAGTCAGAC
    GGAGATGAGG AAAAGACATC AGTGAACTCT GAAGAGAAAA TGGAGCAGCT GCAAAGTTCA
    GACTTGAAGT TCTACTCTCT GATGTTGTCA GCCAATGGGC ATGTGGATCC TTGTGGCACT
    TTGCCTCCAC CAATCATCAT GCGCACTGCT GCCACACCCA TGCCCACTCC AAAGAGCTCT
    CTGGGGCCCG AGTCTGTGTT TGACTTGACC GAGTCCAGAC GGGGCAGTGC TGTCTCCATA
    GATCCAAATG CTTTTGATCA GAAGTCCCTG TCCAGTCCAA GAAGATCACC ATGCCACCCC
    CGTGGCTCGG GCAGTAACTG GGGCAGCCGC AGGTCGTCCT GGAACAGCTT AGGACGAGCG
    CCAAGTCTGA AAAGGAAGGA CACATCTGGG GAGCGCGAGT CTCTGCTGTC CGGGGAAGGC
    AGGGGCAGCT TTGAGGATGA CGACATAGAG GCGGAGAAGC TGACTAACAT CAGCGGAGGC
    TCCCTGCAGC ACCCCAGCTC TCTGGACATC CCAGAACTGC CTCAAATGGG GGAATACCTC
    GACTGCAATG GACGAAGCCA ACACATACTC GCTGACCTGT CTGCCAGTCT CAACAAGGAG
    GACTCCATCG CTGAGGAGGA CTTGGATGAT AGCTTCTGTT TCCGTCTCAG GAGGACCCTG
    GCAGCTTATA AACCCAAGTG GTGCAAAGAT CATGAGGAAT GGTCACTTTA CCTGTTCTCT
    CCTCATAACA AGTTTCGGAT GATGTGTCAA AAACTCATCT CTCACAAGAT GTTTGATTAT
    GTAGTTTTAG TATTCATCTT CCTAAACTGC ATCACTATTG CTCTGGAGAG ACCCCATATT
    CAACAATCTG AGCGATTATT TCTCCTCGTC TCAAACTATG TTTTCACTGT GATATTTGTT
    GCTGAAATGA CAGTGAAGGT TGTGGCCCTT GGTTTCTACT CCGGGAACCA GAGTTATCTA
    AAGAGCACAT GGAATGTTTT GGATGGGGTG CTGGTCTTTG TGTCCCTCAT TGACATTCTG
    GTCTCTCTGG CCTGGACTGG AAACCGAATC TTTGGCATCC TGAGGGTTCT TCGCCTGCTG
    CGAACTCTGC GGCCACTGAG AGTAATTAGT CGTGCTCCTG GTCTGAAGCT GGTAGTAGAA
    ACGTTGATAA CGTCCCTGAG GCCCATTGGG AACATTGTGC TGATCTGCTG TGCTTTCTTT
    ATTGTTTTCG GCATTCTTGG AGTTCAGCTC TTCAAAGGAA AGTTTTTCCA CTGTGAAGGC
    GGCGATACCA GAAACATCAC CAATAAGTCT GACTGTCTTC AGGCAAACCT CAAATGGATC
    AGAAGAAAGT ACAATTTTGA CAACTTGGGA CAGGCTCTCA TGTCTCTTTT CGTGCTTTCC
    TGTAAGGATG GATGGGTCAA TATAATGTAT GATGGGTTGG ATGCTGTTGG TGTGGACCAG
    CAGCCTGAGC GCAACCACAA CCCCTGGATG CTGTTATATT TTATTTCCTT TCTGCTAATC
    GTCAGCTTCT TCGTCCTGAA TATGTTTGTC GGAGTAGTGG TGGAAAACTT CCACAAGTGC
    CGTCAAGACC AGGAGGAAGT GGAGGCGCGT CTGCTGGAGT TGAAGAGGCA AAAACTGATG
    GAGAAAAAGC GCAGGAGTAA GGAGAATTGC GGGTCTGAGG CACTCCGGAG ACCATATTAT
    GCTGACTATT CTCCAGCCCG GCTTTACATC CACACACTGT GCACCAACCA CTATTTGGAC
    CTGTTTATAA CCGGCATCAT CTGCATCAAC GTAGTTACCA TGTCCATCGA GCATTTCAAT
    CAGCCTTCTT ATCTTGACGA GGCACTTAAA TACTGTAACT ATGTATTTAC AATCATCTTC
    ATAATTGAGG CTCTGCTTAA ACTCGTGGCA TTTGGAATAC GGAGATTCTT CAAAGACAGA
    TGGAACCAGC TGGATCTTGC AATAGTCCTA CTCTCTATCA TGGGCATCAC TCTGGAGGAG
    ATTAAGATGA ATGCAGCGCT GCCCATCAAC CCCACCATCA TCCGTATTAT GAGAGTGCTA
    AGAATCGCAA GAGTGCTGAA GCTGTTAAAG ATGGCTACAG GCATGAGATC CCTACTCGAC
    ACCGTAATGC AAGCTTTACC ACAGGTGGGA AATCTGGGCT TGCTGTTTAT GCTTCTCTTC
    TTCATTTATG CGGCGCTTGG CGTGGAGCTT TTTGGGAAAC TAGAATGCAC AGAGAACAAT
    CCGTGTGAAG GATTAAGTCC ACATGCCACG TTTGAGAACT TTGGAATGGC ATTTTTGACA
    CTTTTCCGAG TATCAACTGG GGACAATTGG AATGGAATAA TGAAGGACAC TCTGCGAGAG
    TGCCTTCCTT CAGAGACGCA ATGCCTCAGC TATCTACCCT GGGTCTCTCC AATCTATTTT
    GTAACCTTTG TTCTAATGGC TCAGTTTGTG CTGGTTAACG TGGTCGTAGC TGTGTTGATG
    AAACATCTGG AGGAGAGCAA TAAAGAAGCT AAAGAGGATG CAGAAATGGA TGCTGCGATC
    AAGTTGGCAA TTTTGAAAGA AACTCGCCGT CTCAGCACCA TCTCAGCCAG TGCCACTGCG
    GGACCAGAAG GCAGGGCAAC TTATATCGAG CCACACCCAG ATTCACCTGA GCAGGATGAG
    GGGATGCAAA GAACACAGGG GAATTTGCTC TCTCCGCGGA AAATGTCAGT GTCAAGGATG
    CACTCTTTAC CCAATGACAG CTACATGTTT CGCCCTGTGA GGCCAGCCAG TGCTCCGTAT
    CCTCTAGAAG AAGTGGAGAC CTGTCAAGAT TATCTTGAAT TAGGTTCCAT CACCTCGGTT
    CACTCCCAGC CATGCGAAAG CCTTTCCCTG CTCCAGGTCC CTGGTGTTCC TCCCCATTCC
    CAGTTCAACT GCCTCCCCAA GATCCCTCCT CCCATGCCCT CTCCAACCCA CAGCATAGAC
    AGGATTTTAC GCAGACAGAG GGCAATTAAA AATGACTCCA TTGACATGCA TAATTTCGAC
    TCCAAAGAAA ATGTGGAAAG ACAGAAAGGG ATAACCACAG GCAGCTTCAA GCTGCATGTT
    CCTCAAATCA CCGGCATCTC TCAGGGGTCT CCCTTGCTGC CACCACGTGG ACCTAGTGTC
    CACACCTTCC AGCAGCCGCA CAGCCAGCAC AATATCTCTT CTCGACCTCC CAGTCTGGCC
    AGCAGCAGCA GAAGCACCAG CCCCAGTGGC TCAATGTTTG ACTCCGCTGA CCCAACCGAT
    GAGGAGGTGC GCCACATCAA TAGCACAGCC CGCCTGTGGA TCGGGGTTCA TGCAGAAGCA
    AGGAGTCACT CCCTCTCTCC TTACAGCACC TCAGGGACGC TGCTTCCCAT CCCCGTGAAA
    AACTGCAGCA GTGCTGCCAA CTTAACCATC AAAGACCCCA AGAAGTGCTT CAGTGTGGAC
    ACTGAGGGTT TTCTGGAAAG ACCCTACGCT TCTGATGACC ATCGCAGGCA CTCGATTGAG
    ATTTGCTCCT CAGGAGACGA TGGGCTGTTT GGTCAAGAAC TCTGTGAAAA GAGGCACGTT
    CCCACGCGTG GAAAGTCTTT GCACATTCCC CGGAAGACAA AGATGAGCCC TCCCTGCATC
    TCCATCGAGC CCCCATTTGA AAAGGATGTT TCCCCCTCTC ATGCGTCCAT GAAAAAGTTG
    TCGGATAGCA TGTTACTGCG GAGGAGGACA CCATCCTATG ACCTGGCCCT GCAGCGGGAC
    TCTCTGGACT TGCCGGAGAA CCAGCTGTCA ACCCAGCCAC TCATCAAAGT AGAGCGACAT
    CCCTCTCACT GTGCCGAGTA TCTGTCCCTC CCACACCCCG CCCCAATGAG CGGTCTGGCA
    GGCGTTTTGT GTGAAAGCAC GCAGCCGCCC CCCTTTGATA GCAGATATGA AGAATCCACG
    GACTTCGGCT CTGTAAACAG GACAGCTCAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297960.1
    CDS485..7057
    Translation

    Target ORF information:

    RefSeq Version XM_009299685.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, T type, alpha 1H subunit a (cacna1ha), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299685.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGCTGGTGA TTCTGCTGAA CTGTGTGACT CTGGGCATGT ATCAGCCATG TGAGGACCTC 
    AAGTGCCAGT CTGAGTGGTG TATCGTCCTG CAGGCGTTTG ATGACTGCAT CTTCGCCTTC
    TTCGCTGTGG AGATGGTCAT CAAAATGATC GCCCTGGGGA TTTTTGGAAT AAATGGTTAT
    CTTGGCGACA CGTGGAACCG CCTGGACTTC TTCATTGTCA TGGCAGGGAT GATGGAGTAT
    TCACTGGACG GTCATAACGC CAGCCTGTCA GCCATCCGGA CTGTGCGAGT GCTCAGGCCC
    CTGCGAGCCA TCAACAGAGT GCCCAGTATG AGGATACTGG TCACTCTGCT TCTAGATACT
    CTGCCTATGT TAGGCAACGT CCTCCTGCTG TGCTTTTTTG TCTTCTTTAT ATTCGGAATT
    GTCGGGGTGC AGCTGTGGGC TGGACTTCTT AGGAATCGGT GTTTCATGCC AAGCAAAGTG
    AGAGACCAAT ATAATCTGTC GTATATGAGT CTGTACTATG AGAACGAAGA TGGTGAAGAC
    AACCCCTTCA TCTGCTCCTC CAGTAAGGAC AACGGCATGA AGCGCTGCAG TGCTGTGCCA
    CCCCTTAAAG AGGGTGTTGA GTGCACTTTA AATGCATCTT TTCTGGGACA TGTCTACTCT
    GGCCTGAGCG GGACAGGCAA CTACAGCTGT GTGAACTGGA ACCAGTATTA CAGTGAATGC
    AAGCCCGGAG ACTTAAACCC GCACAAAGGC GCAGTAAACT TTGATAACAT TGGATATGCC
    TGGATCGCTA TATTCCAGGT TATTACATTG GAAGGATGGG TGGACATCAT GTATTACGTA
    ATGGATGCCC ACTCCTTTTA CAATTTTATA TATTTTATAT TGCTTATTAT AGTGGGTTCC
    TTCTTCATGA TCAACCTGTG CCTGGTTGTA ATAGCAACCC AGTTCGCAGA AACCAAACAG
    CGCGAGAACA TGCTGATGAA GGAGCAGCGT GCACGATACC GCTCCAACGA CAGCACCCTC
    GCCAGCTACT CTGAGCCGGG CAGCTGCTAC GAAGAGATGC TCAAGTACGT CAGCCACCTG
    TACCGCAAGG TGAAGCGCCG CCTCTCCCGC ATCTACAACA GCTGGCAGAG CAAGCGGCGA
    AAGAAAGTGA ACCCCAACAC CGGGGCTGGC GGAGGTGCCA ATGGTCACAG TAGGCACAGG
    GGTTACGGAC ACTGGGTACA GTCCATTCAC AGCCTCATCC AGCAGCACCA GCGACAGCAC
    TGTCACCTCA GCAATGGCAG CCCCAGTCCT GTGGCCACTA CGGGCTCCAG TGAGGCTCTG
    GAAATGAGGT CCATCACAGC GGGTCAGCAG CTGACCGTGT CCTCTCAGCC AACCGCCACG
    CACAATCTTT CTTCCAGCAC ACAATCAGTG CATAGCATTT ATCAAGGTGA CTTTCGTGAG
    GCCCCGCAGG AGCGAACACC CACTAATGTT GCTGTTCCGG CACTCAGCCG ACTCAATGGG
    GGGATGAACT ATCCCACAAT TTTGCCGTCC CTCATTTGCA ACTATTCAGG TGGAGGTTCA
    TTAGGGAAAG AGAGGGGGAA GTCGGATGCA AATACAGGAC ATGAGGTGAT ACAGGACTTT
    GACAAGCTGC ACCAGCAAAT TGAAGCACAT AGCCACAGTC GTGTGCTGCT GCAGATGGCT
    GGTGTGGTGC CCACTCAATT ACTTCTTGAG GTCATGAGCT GCCCGTATTG CGTAAGGGCC
    CTTGAGAGCA TGGATCTGGA GATTGGTGAT TCAGACTACC CTCGATCAGA AGGGGACATG
    GTGTATGAGT TCTCATATGA TGCCGGGTTC ATAACCGGCC GTTCAGCACA TCATAAAATG
    CAGGAGAAAC AAAACCGTCT GGTTCAATTC TGGGAGGACT TCAGGGAAAG ACTGACCCGT
    ATAGTGGATA GCAAATACTT TAACCGGGGA ATTATGATCG CCATCCTCAT CAACACCTTG
    AGTATGGGGA TTGAATACCA TGAGCAGCCC GAGGAGCTGA CCAACATCCT GGAGATCAGC
    AACATCGTCT TCACTAGCAT GTTTGTCCTC GAAATGCTCT TCAAACTGCT GGCCTTTGGC
    ATCTTCGGCT ATATAAGGAA CCCATATAAC ATCTTTGATG GGGTTATTGT TGTCATAAGT
    GTATGGGAGA TCATCGGTCA TGCGGATGGT GGTCTCTCAG TGCTGAGGAC TTTTCGTCTC
    CTGAGGGTGC TCAAACTGGT GCGCTTCCTC CCTGCCCTGC GCAGACAGCT GCTTGTCCTG
    ATGAAGACCA TGGACAATGT GGCAACATTT TGCATGCTAC TCATGCTCTT CATCTTCACT
    TTCAGCATAC TCGGAATGCA TCTGTTCGGA TGCAAATTCA GCCTGAAGAT GGAGAATGGA
    GACACTATTC CGGACAGGAA AAACTTTGAT TCCCTGCTGT GGGCCATTGT CACTGTGTTT
    CAGATTCTGA CCCAGGAAGA CTGGAACGTG GTCCTCTATA ATGGAATGGC TTCCACCAGC
    CCATGGGCGG CTCTTTATTT TGTTGCCCTC ATGACGTTTG GGAATTATGT GCTCTTTAAT
    CTACTGGTGG CCATTTTAGT GGAAGGGTTT CAGGCGGAGG GAGATGCTAA CAAGTCAGAC
    GGAGATGAGG AAAAGACATC AGTGAACTCT GAAGAGAAAA TGGAGCAGCT GCAAAGTTCA
    GACTTGAAGT TCTACTCTCT GATGTTGTCA GCCAATGGGC ATGTGGATCC TTGTGGCACT
    TTGCCTCCAC CAATCATCAT GCGCACTGCT GCCACACCCA TGCCCACTCC AAAGAGCTCT
    CTGGGGCCCG AGTCTGTGTT TGACTTGACC GAGTCCAGAC GGGGCAGTGC TGTCTCCATA
    GATCCAAATG CTTTTGATCA GAAGTCCCTG TCCAGTCCAA GAAGATCACC ATGCCACCCC
    CGTGGCTCGG GCAGTAACTG GGGCAGCCGC AGGTCGTCCT GGAACAGCTT AGGACGAGCG
    CCAAGTCTGA AAAGGAAGGA CACATCTGGG GAGCGCGAGT CTCTGCTGTC CGGGGAAGGC
    AGGGGCAGCT TTGAGGATGA CGACATAGAG GCGGAGAAGC TGACTAACAT CAGCGGAGGC
    TCCCTGCAGC ACCCCAGCTC TCTGGACATC CCAGAACTGC CTCAAATGGG GGAATACCTC
    GACTGCAATG GACGAAGCCA ACACATACTC GCTGACCTGT CTGCCAGTCT CAACAAGGAG
    GACTCCATCG CTGAGGAGGA CTTGGATGAT AGCTTCTGTT TCCGTCTCAG GAGGACCCTG
    GCAGCTTATA AACCCAAGTG GTGCAAAGAT CATGAGGAAT GGTCACTTTA CCTGTTCTCT
    CCTCATAACA AGTTTCGGAT GATGTGTCAA AAACTCATCT CTCACAAGAT GTTTGATTAT
    GTAGTTTTAG TATTCATCTT CCTAAACTGC ATCACTATTG CTCTGGAGAG ACCCCATATT
    CAACAATCTG AGCGATTATT TCTCCTCGTC TCAAACTATG TTTTCACTGT GATATTTGTT
    GCTGAAATGA CAGTGAAGGT TGTGGCCCTT GGTTTCTACT CCGGGAACCA GAGTTATCTA
    AAGAGCACAT GGAATGTTTT GGATGGGGTG CTGGTCTTTG TGTCCCTCAT TGACATTCTG
    GTCTCTCTGG CCTGGACTGG AAACCGAATC TTTGGCATCC TGAGGGTTCT TCGCCTGCTG
    CGAACTCTGC GGCCACTGAG AGTAATTAGT CGTGCTCCTG GTCTGAAGCT GGTAGTAGAA
    ACGTTGATAA CGTCCCTGAG GCCCATTGGG AACATTGTGC TGATCTGCTG TGCTTTCTTT
    ATTGTTTTCG GCATTCTTGG AGTTCAGCTC TTCAAAGGAA AGTTTTTCCA CTGTGAAGGC
    GGCGATACCA GAAACATCAC CAATAAGTCT GACTGTCTTC AGGCAAACCT CAAATGGATC
    AGAAGAAAGT ACAATTTTGA CAACTTGGGA CAGGCTCTCA TGTCTCTTTT CGTGCTTTCC
    TGTAAGGATG GATGGGTCAA TATAATGTAT GATGGGTTGG ATGCTGTTGG TGTGGACCAG
    CAGCCTGAGC GCAACCACAA CCCCTGGATG CTGTTATATT TTATTTCCTT TCTGCTAATC
    GTCAGCTTCT TCGTCCTGAA TATGTTTGTC GGAGTAGTGG TGGAAAACTT CCACAAGTGC
    CGTCAAGACC AGGAGGAAGT GGAGGCGCGT CTGCTGGAGT TGAAGAGGCA AAAACTGATG
    GAGAAAAAGC GCAGGAGTAA GGAGAATTGC GGGTCTGAGG CACTCCGGAG ACCATATTAT
    GCTGACTATT CTCCAGCCCG GCTTTACATC CACACACTGT GCACCAACCA CTATTTGGAC
    CTGTTTATAA CCGGCATCAT CTGCATCAAC GTAGTTACCA TGTCCATCGA GCATTTCAAT
    CAGCCTTCTT ATCTTGACGA GGCACTTAAA TACTGTAACT ATGTATTTAC AATCATCTTC
    ATAATTGAGG CTCTGCTTAA ACTCGTGGCA TTTGGAATAC GGAGATTCTT CAAAGACAGA
    TGGAACCAGC TGGATCTTGC AATAGTCCTA CTCTCTATCA TGGGCATCAC TCTGGAGGAG
    ATTAAGATGA ATGCAGCGCT GCCCATCAAC CCCACCATCA TCCGTATTAT GAGAGTGCTA
    AGAATCGCAA GAGTGCTGAA GCTGTTAAAG ATGGCTACAG GCATGAGATC CCTACTCGAC
    ACCGTAATGC AAGCTTTACC ACAGGTGGGA AATCTGGGCT TGCTGTTTAT GCTTCTCTTC
    TTCATTTATG CGGCGCTTGG CGTGGAGCTT TTTGGGAAAC TAGAATGCAC AGAGAACAAT
    CCGTGTGAAG GATTAAGTCC ACATGCCACG TTTGAGAACT TTGGAATGGC ATTTTTGACA
    CTTTTCCGAG TATCAACTGG GGACAATTGG AATGGAATAA TGAAGGACAC TCTGCGAGAG
    TGCCTTCCTT CAGAGACGCA ATGCCTCAGC TATCTACCCT GGGTCTCTCC AATCTATTTT
    GTAACCTTTG TTCTAATGGC TCAGTTTGTG CTGGTTAACG TGGTCGTAGC TGTGTTGATG
    AAACATCTGG AGGAGAGCAA TAAAGAAGCT AAAGAGGATG CAGAAATGGA TGCTGCGATC
    AAGTTGGCAA TTTTGAAAGA AACTCGCCGT CTCAGCACCA TCTCAGCCAG TGCCACTGCG
    GGACCAGAAG GCAGGGCAAC TTATATCGAG CCACACCCAG ATTCACCTGA GCAGGATGAG
    GGGATGCAAA GAACACAGGG GAATTTGCTC TCTCCGCGGA AAATGTCAGT GTCAAGGATG
    CACTCTTTAC CCAATGACAG CTACATGTTT CGCCCTGTGA GGCCAGCCAG TGCTCCGTAT
    CCTCTAGAAG AAGTGGAGAC CTGTCAAGAT TATCTTGAAT TAGGTTCCAT CACCTCGGTT
    CACTCCCAGC CATGCGAAAG CCTTTCCCTG CTCCAGGTCC CTGGTGTTCC TCCCCATTCC
    CAGTTCAACT GCCTCCCCAA GATCCCTCCT CCCATGCCCT CTCCAACCCA CAGCATAGAC
    AGGATTTTAC GCAGACAGAG GGCAATTAAA AATGACTCCA TTGACATGCA TAATTTCGAC
    TCCAAAGAAA ATGTGGAAAG ACAGAAAGGG ATAACCACAG GCAGCTTCAA GCTGCATGTT
    CCTCAAATCA CCGGCATCTC TCAGGGGTCT CCCTTGCTGC CACCACGTGG ACCTAGTGTC
    CACACCTTCC AGCAGCCGCA CAGCCAGCAC AATATCTCTT CTCGACCTCC CAGTCTGGCC
    AGCAGCAGCA GAAGCACCAG CCCCAGTGGC TCAATGTTTG ACTCCGCTGA CCCAACCGAT
    GAGGAGGTGC GCCACATCAA TAGCACAGCC CGCCTGTGGA TCGGGGTTCA TGCAGAAGCA
    AGGAGTCACT CCCTCTCTCC TTACAGCACC TCAGGGACGC TGCTTCCCAT CCCCGTGAAA
    AACTGCAGCA GTGCTGCCAA CTTAACCATC AAAGACCCCA AGAAGTGCTT CAGTGTGGAC
    ACTGAGGGTT TTCTGGAAAG ACCCTACGCT TCTGATGACC ATCGCAGGCA CTCGATTGAG
    ATTTGCTCCT CAGGAGACGA TGGGCTGTTT GGTCAAGAAC TCTGTGAAAA GAGGCACGTT
    CCCACGCGTG GAAAGTCTTT GCACATTCCC CGGAAGACAA AGATGAGCCC TCCCTGCATC
    TCCATCGAGC CCCCATTTGA AAAGGATGTT TCCCCCTCTC ATGCGTCCAT GAAAAAGTTG
    TCGGATAGCA TGTTACTGCG GAGGAGGACA CCATCCTATG ACCTGGCCCT GCAGCGGGAC
    TCTCTGGACT TGCCGGAGAA CCAGCTGTCA ACCCAGCCAC TCATCAAAGT AGAGCGACAT
    CCCTCTCACT GTGCCGAGTA TCTGTCCCTC CCACACCCCG CCCCAATGAG CGGTCTGGCA
    GGCGTTTTGT GTGAAAGCAC GCAGCCGCCC CCCTTTGATA GCAGATATGA AGAATCCACG
    GACTTCGGCT CTGTAAACAG GACAGCTCAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43367
    Clone ID Related Accession (Same CDS sequence) XM_009299685.3 , XM_009299685.2
    Accession Version XM_009299685.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6573bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent T-type calcium channel subunit alpha-1H isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299685.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGCTGGTGA TTCTGCTGAA CTGTGTGACT CTGGGCATGT ATCAGCCATG TGAGGACCTC 
    AAGTGCCAGT CTGAGTGGTG TATCGTCCTG CAGGCGTTTG ATGACTGCAT CTTCGCCTTC
    TTCGCTGTGG AGATGGTCAT CAAAATGATC GCCCTGGGGA TTTTTGGAAT AAATGGTTAT
    CTTGGCGACA CGTGGAACCG CCTGGACTTC TTCATTGTCA TGGCAGGGAT GATGGAGTAT
    TCACTGGACG GTCATAACGC CAGCCTGTCA GCCATCCGGA CTGTGCGAGT GCTCAGGCCC
    CTGCGAGCCA TCAACAGAGT GCCCAGTATG AGGATACTGG TCACTCTGCT TCTAGATACT
    CTGCCTATGT TAGGCAACGT CCTCCTGCTG TGCTTTTTTG TCTTCTTTAT ATTCGGAATT
    GTCGGGGTGC AGCTGTGGGC TGGACTTCTT AGGAATCGGT GTTTCATGCC AAGCAAAGTG
    AGAGACCAAT ATAATCTGTC GTATATGAGT CTGTACTATG AGAACGAAGA TGGTGAAGAC
    AACCCCTTCA TCTGCTCCTC CAGTAAGGAC AACGGCATGA AGCGCTGCAG TGCTGTGCCA
    CCCCTTAAAG AGGGTGTTGA GTGCACTTTA AATGCATCTT TTCTGGGACA TGTCTACTCT
    GGCCTGAGCG GGACAGGCAA CTACAGCTGT GTGAACTGGA ACCAGTATTA CAGTGAATGC
    AAGCCCGGAG ACTTAAACCC GCACAAAGGC GCAGTAAACT TTGATAACAT TGGATATGCC
    TGGATCGCTA TATTCCAGGT TATTACATTG GAAGGATGGG TGGACATCAT GTATTACGTA
    ATGGATGCCC ACTCCTTTTA CAATTTTATA TATTTTATAT TGCTTATTAT AGTGGGTTCC
    TTCTTCATGA TCAACCTGTG CCTGGTTGTA ATAGCAACCC AGTTCGCAGA AACCAAACAG
    CGCGAGAACA TGCTGATGAA GGAGCAGCGT GCACGATACC GCTCCAACGA CAGCACCCTC
    GCCAGCTACT CTGAGCCGGG CAGCTGCTAC GAAGAGATGC TCAAGTACGT CAGCCACCTG
    TACCGCAAGG TGAAGCGCCG CCTCTCCCGC ATCTACAACA GCTGGCAGAG CAAGCGGCGA
    AAGAAAGTGA ACCCCAACAC CGGGGCTGGC GGAGGTGCCA ATGGTCACAG TAGGCACAGG
    GGTTACGGAC ACTGGGTACA GTCCATTCAC AGCCTCATCC AGCAGCACCA GCGACAGCAC
    TGTCACCTCA GCAATGGCAG CCCCAGTCCT GTGGCCACTA CGGGCTCCAG TGAGGCTCTG
    GAAATGAGGT CCATCACAGC GGGTCAGCAG CTGACCGTGT CCTCTCAGCC AACCGCCACG
    CACAATCTTT CTTCCAGCAC ACAATCAGTG CATAGCATTT ATCAAGGTGA CTTTCGTGAG
    GCCCCGCAGG AGCGAACACC CACTAATGTT GCTGTTCCGG CACTCAGCCG ACTCAATGGG
    GGGATGAACT ATCCCACAAT TTTGCCGTCC CTCATTTGCA ACTATTCAGG TGGAGGTTCA
    TTAGGGAAAG AGAGGGGGAA GTCGGATGCA AATACAGGAC ATGAGGTGAT ACAGGACTTT
    GACAAGCTGC ACCAGCAAAT TGAAGCACAT AGCCACAGTC GTGTGCTGCT GCAGATGGCT
    GGTGTGGTGC CCACTCAATT ACTTCTTGAG GTCATGAGCT GCCCGTATTG CGTAAGGGCC
    CTTGAGAGCA TGGATCTGGA GATTGGTGAT TCAGACTACC CTCGATCAGA AGGGGACATG
    GTGTATGAGT TCTCATATGA TGCCGGGTTC ATAACCGGCC GTTCAGCACA TCATAAAATG
    CAGGAGAAAC AAAACCGTCT GGTTCAATTC TGGGAGGACT TCAGGGAAAG ACTGACCCGT
    ATAGTGGATA GCAAATACTT TAACCGGGGA ATTATGATCG CCATCCTCAT CAACACCTTG
    AGTATGGGGA TTGAATACCA TGAGCAGCCC GAGGAGCTGA CCAACATCCT GGAGATCAGC
    AACATCGTCT TCACTAGCAT GTTTGTCCTC GAAATGCTCT TCAAACTGCT GGCCTTTGGC
    ATCTTCGGCT ATATAAGGAA CCCATATAAC ATCTTTGATG GGGTTATTGT TGTCATAAGT
    GTATGGGAGA TCATCGGTCA TGCGGATGGT GGTCTCTCAG TGCTGAGGAC TTTTCGTCTC
    CTGAGGGTGC TCAAACTGGT GCGCTTCCTC CCTGCCCTGC GCAGACAGCT GCTTGTCCTG
    ATGAAGACCA TGGACAATGT GGCAACATTT TGCATGCTAC TCATGCTCTT CATCTTCACT
    TTCAGCATAC TCGGAATGCA TCTGTTCGGA TGCAAATTCA GCCTGAAGAT GGAGAATGGA
    GACACTATTC CGGACAGGAA AAACTTTGAT TCCCTGCTGT GGGCCATTGT CACTGTGTTT
    CAGATTCTGA CCCAGGAAGA CTGGAACGTG GTCCTCTATA ATGGAATGGC TTCCACCAGC
    CCATGGGCGG CTCTTTATTT TGTTGCCCTC ATGACGTTTG GGAATTATGT GCTCTTTAAT
    CTACTGGTGG CCATTTTAGT GGAAGGGTTT CAGGCGGAGG GAGATGCTAA CAAGTCAGAC
    GGAGATGAGG AAAAGACATC AGTGAACTCT GAAGAGAAAA TGGAGCAGCT GCAAAGTTCA
    GACTTGAAGT TCTACTCTCT GATGTTGTCA GCCAATGGGC ATGTGGATCC TTGTGGCACT
    TTGCCTCCAC CAATCATCAT GCGCACTGCT GCCACACCCA TGCCCACTCC AAAGAGCTCT
    CTGGGGCCCG AGTCTGTGTT TGACTTGACC GAGTCCAGAC GGGGCAGTGC TGTCTCCATA
    GATCCAAATG CTTTTGATCA GAAGTCCCTG TCCAGTCCAA GAAGATCACC ATGCCACCCC
    CGTGGCTCGG GCAGTAACTG GGGCAGCCGC AGGTCGTCCT GGAACAGCTT AGGACGAGCG
    CCAAGTCTGA AAAGGAAGGA CACATCTGGG GAGCGCGAGT CTCTGCTGTC CGGGGAAGGC
    AGGGGCAGCT TTGAGGATGA CGACATAGAG GCGGAGAAGC TGACTAACAT CAGCGGAGGC
    TCCCTGCAGC ACCCCAGCTC TCTGGACATC CCAGAACTGC CTCAAATGGG GGAATACCTC
    GACTGCAATG GACGAAGCCA ACACATACTC GCTGACCTGT CTGCCAGTCT CAACAAGGAG
    GACTCCATCG CTGAGGAGGA CTTGGATGAT AGCTTCTGTT TCCGTCTCAG GAGGACCCTG
    GCAGCTTATA AACCCAAGTG GTGCAAAGAT CATGAGGAAT GGTCACTTTA CCTGTTCTCT
    CCTCATAACA AGTTTCGGAT GATGTGTCAA AAACTCATCT CTCACAAGAT GTTTGATTAT
    GTAGTTTTAG TATTCATCTT CCTAAACTGC ATCACTATTG CTCTGGAGAG ACCCCATATT
    CAACAATCTG AGCGATTATT TCTCCTCGTC TCAAACTATG TTTTCACTGT GATATTTGTT
    GCTGAAATGA CAGTGAAGGT TGTGGCCCTT GGTTTCTACT CCGGGAACCA GAGTTATCTA
    AAGAGCACAT GGAATGTTTT GGATGGGGTG CTGGTCTTTG TGTCCCTCAT TGACATTCTG
    GTCTCTCTGG CCTGGACTGG AAACCGAATC TTTGGCATCC TGAGGGTTCT TCGCCTGCTG
    CGAACTCTGC GGCCACTGAG AGTAATTAGT CGTGCTCCTG GTCTGAAGCT GGTAGTAGAA
    ACGTTGATAA CGTCCCTGAG GCCCATTGGG AACATTGTGC TGATCTGCTG TGCTTTCTTT
    ATTGTTTTCG GCATTCTTGG AGTTCAGCTC TTCAAAGGAA AGTTTTTCCA CTGTGAAGGC
    GGCGATACCA GAAACATCAC CAATAAGTCT GACTGTCTTC AGGCAAACCT CAAATGGATC
    AGAAGAAAGT ACAATTTTGA CAACTTGGGA CAGGCTCTCA TGTCTCTTTT CGTGCTTTCC
    TGTAAGGATG GATGGGTCAA TATAATGTAT GATGGGTTGG ATGCTGTTGG TGTGGACCAG
    CAGCCTGAGC GCAACCACAA CCCCTGGATG CTGTTATATT TTATTTCCTT TCTGCTAATC
    GTCAGCTTCT TCGTCCTGAA TATGTTTGTC GGAGTAGTGG TGGAAAACTT CCACAAGTGC
    CGTCAAGACC AGGAGGAAGT GGAGGCGCGT CTGCTGGAGT TGAAGAGGCA AAAACTGATG
    GAGAAAAAGC GCAGGAGTAA GGAGAATTGC GGGTCTGAGG CACTCCGGAG ACCATATTAT
    GCTGACTATT CTCCAGCCCG GCTTTACATC CACACACTGT GCACCAACCA CTATTTGGAC
    CTGTTTATAA CCGGCATCAT CTGCATCAAC GTAGTTACCA TGTCCATCGA GCATTTCAAT
    CAGCCTTCTT ATCTTGACGA GGCACTTAAA TACTGTAACT ATGTATTTAC AATCATCTTC
    ATAATTGAGG CTCTGCTTAA ACTCGTGGCA TTTGGAATAC GGAGATTCTT CAAAGACAGA
    TGGAACCAGC TGGATCTTGC AATAGTCCTA CTCTCTATCA TGGGCATCAC TCTGGAGGAG
    ATTAAGATGA ATGCAGCGCT GCCCATCAAC CCCACCATCA TCCGTATTAT GAGAGTGCTA
    AGAATCGCAA GAGTGCTGAA GCTGTTAAAG ATGGCTACAG GCATGAGATC CCTACTCGAC
    ACCGTAATGC AAGCTTTACC ACAGGTGGGA AATCTGGGCT TGCTGTTTAT GCTTCTCTTC
    TTCATTTATG CGGCGCTTGG CGTGGAGCTT TTTGGGAAAC TAGAATGCAC AGAGAACAAT
    CCGTGTGAAG GATTAAGTCC ACATGCCACG TTTGAGAACT TTGGAATGGC ATTTTTGACA
    CTTTTCCGAG TATCAACTGG GGACAATTGG AATGGAATAA TGAAGGACAC TCTGCGAGAG
    TGCCTTCCTT CAGAGACGCA ATGCCTCAGC TATCTACCCT GGGTCTCTCC AATCTATTTT
    GTAACCTTTG TTCTAATGGC TCAGTTTGTG CTGGTTAACG TGGTCGTAGC TGTGTTGATG
    AAACATCTGG AGGAGAGCAA TAAAGAAGCT AAAGAGGATG CAGAAATGGA TGCTGCGATC
    AAGTTGGCAA TTTTGAAAGA AACTCGCCGT CTCAGCACCA TCTCAGCCAG TGCCACTGCG
    GGACCAGAAG GCAGGGCAAC TTATATCGAG CCACACCCAG ATTCACCTGA GCAGGATGAG
    GGGATGCAAA GAACACAGGG GAATTTGCTC TCTCCGCGGA AAATGTCAGT GTCAAGGATG
    CACTCTTTAC CCAATGACAG CTACATGTTT CGCCCTGTGA GGCCAGCCAG TGCTCCGTAT
    CCTCTAGAAG AAGTGGAGAC CTGTCAAGAT TATCTTGAAT TAGGTTCCAT CACCTCGGTT
    CACTCCCAGC CATGCGAAAG CCTTTCCCTG CTCCAGGTCC CTGGTGTTCC TCCCCATTCC
    CAGTTCAACT GCCTCCCCAA GATCCCTCCT CCCATGCCCT CTCCAACCCA CAGCATAGAC
    AGGATTTTAC GCAGACAGAG GGCAATTAAA AATGACTCCA TTGACATGCA TAATTTCGAC
    TCCAAAGAAA ATGTGGAAAG ACAGAAAGGG ATAACCACAG GCAGCTTCAA GCTGCATGTT
    CCTCAAATCA CCGGCATCTC TCAGGGGTCT CCCTTGCTGC CACCACGTGG ACCTAGTGTC
    CACACCTTCC AGCAGCCGCA CAGCCAGCAC AATATCTCTT CTCGACCTCC CAGTCTGGCC
    AGCAGCAGCA GAAGCACCAG CCCCAGTGGC TCAATGTTTG ACTCCGCTGA CCCAACCGAT
    GAGGAGGTGC GCCACATCAA TAGCACAGCC CGCCTGTGGA TCGGGGTTCA TGCAGAAGCA
    AGGAGTCACT CCCTCTCTCC TTACAGCACC TCAGGGACGC TGCTTCCCAT CCCCGTGAAA
    AACTGCAGCA GTGCTGCCAA CTTAACCATC AAAGACCCCA AGAAGTGCTT CAGTGTGGAC
    ACTGAGGGTT TTCTGGAAAG ACCCTACGCT TCTGATGACC ATCGCAGGCA CTCGATTGAG
    ATTTGCTCCT CAGGAGACGA TGGGCTGTTT GGTCAAGAAC TCTGTGAAAA GAGGCACGTT
    CCCACGCGTG GAAAGTCTTT GCACATTCCC CGGAAGACAA AGATGAGCCC TCCCTGCATC
    TCCATCGAGC CCCCATTTGA AAAGGATGTT TCCCCCTCTC ATGCGTCCAT GAAAAAGTTG
    TCGGATAGCA TGTTACTGCG GAGGAGGACA CCATCCTATG ACCTGGCCCT GCAGCGGGAC
    TCTCTGGACT TGCCGGAGAA CCAGCTGTCA ACCCAGCCAC TCATCAAAGT AGAGCGACAT
    CCCTCTCACT GTGCCGAGTA TCTGTCCCTC CCACACCCCG CCCCAATGAG CGGTCTGGCA
    GGCGTTTTGT GTGAAAGCAC GCAGCCGCCC CCCTTTGATA GCAGATATGA AGAATCCACG
    GACTTCGGCT CTGTAAACAG GACAGCTCAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297960.1
    CDS19..6591
    Translation

    Target ORF information:

    RefSeq Version XM_009299685.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, T type, alpha 1H subunit a (cacna1ha), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299685.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGCTGGTGA TTCTGCTGAA CTGTGTGACT CTGGGCATGT ATCAGCCATG TGAGGACCTC 
    AAGTGCCAGT CTGAGTGGTG TATCGTCCTG CAGGCGTTTG ATGACTGCAT CTTCGCCTTC
    TTCGCTGTGG AGATGGTCAT CAAAATGATC GCCCTGGGGA TTTTTGGAAT AAATGGTTAT
    CTTGGCGACA CGTGGAACCG CCTGGACTTC TTCATTGTCA TGGCAGGGAT GATGGAGTAT
    TCACTGGACG GTCATAACGC CAGCCTGTCA GCCATCCGGA CTGTGCGAGT GCTCAGGCCC
    CTGCGAGCCA TCAACAGAGT GCCCAGTATG AGGATACTGG TCACTCTGCT TCTAGATACT
    CTGCCTATGT TAGGCAACGT CCTCCTGCTG TGCTTTTTTG TCTTCTTTAT ATTCGGAATT
    GTCGGGGTGC AGCTGTGGGC TGGACTTCTT AGGAATCGGT GTTTCATGCC AAGCAAAGTG
    AGAGACCAAT ATAATCTGTC GTATATGAGT CTGTACTATG AGAACGAAGA TGGTGAAGAC
    AACCCCTTCA TCTGCTCCTC CAGTAAGGAC AACGGCATGA AGCGCTGCAG TGCTGTGCCA
    CCCCTTAAAG AGGGTGTTGA GTGCACTTTA AATGCATCTT TTCTGGGACA TGTCTACTCT
    GGCCTGAGCG GGACAGGCAA CTACAGCTGT GTGAACTGGA ACCAGTATTA CAGTGAATGC
    AAGCCCGGAG ACTTAAACCC GCACAAAGGC GCAGTAAACT TTGATAACAT TGGATATGCC
    TGGATCGCTA TATTCCAGGT TATTACATTG GAAGGATGGG TGGACATCAT GTATTACGTA
    ATGGATGCCC ACTCCTTTTA CAATTTTATA TATTTTATAT TGCTTATTAT AGTGGGTTCC
    TTCTTCATGA TCAACCTGTG CCTGGTTGTA ATAGCAACCC AGTTCGCAGA AACCAAACAG
    CGCGAGAACA TGCTGATGAA GGAGCAGCGT GCACGATACC GCTCCAACGA CAGCACCCTC
    GCCAGCTACT CTGAGCCGGG CAGCTGCTAC GAAGAGATGC TCAAGTACGT CAGCCACCTG
    TACCGCAAGG TGAAGCGCCG CCTCTCCCGC ATCTACAACA GCTGGCAGAG CAAGCGGCGA
    AAGAAAGTGA ACCCCAACAC CGGGGCTGGC GGAGGTGCCA ATGGTCACAG TAGGCACAGG
    GGTTACGGAC ACTGGGTACA GTCCATTCAC AGCCTCATCC AGCAGCACCA GCGACAGCAC
    TGTCACCTCA GCAATGGCAG CCCCAGTCCT GTGGCCACTA CGGGCTCCAG TGAGGCTCTG
    GAAATGAGGT CCATCACAGC GGGTCAGCAG CTGACCGTGT CCTCTCAGCC AACCGCCACG
    CACAATCTTT CTTCCAGCAC ACAATCAGTG CATAGCATTT ATCAAGGTGA CTTTCGTGAG
    GCCCCGCAGG AGCGAACACC CACTAATGTT GCTGTTCCGG CACTCAGCCG ACTCAATGGG
    GGGATGAACT ATCCCACAAT TTTGCCGTCC CTCATTTGCA ACTATTCAGG TGGAGGTTCA
    TTAGGGAAAG AGAGGGGGAA GTCGGATGCA AATACAGGAC ATGAGGTGAT ACAGGACTTT
    GACAAGCTGC ACCAGCAAAT TGAAGCACAT AGCCACAGTC GTGTGCTGCT GCAGATGGCT
    GGTGTGGTGC CCACTCAATT ACTTCTTGAG GTCATGAGCT GCCCGTATTG CGTAAGGGCC
    CTTGAGAGCA TGGATCTGGA GATTGGTGAT TCAGACTACC CTCGATCAGA AGGGGACATG
    GTGTATGAGT TCTCATATGA TGCCGGGTTC ATAACCGGCC GTTCAGCACA TCATAAAATG
    CAGGAGAAAC AAAACCGTCT GGTTCAATTC TGGGAGGACT TCAGGGAAAG ACTGACCCGT
    ATAGTGGATA GCAAATACTT TAACCGGGGA ATTATGATCG CCATCCTCAT CAACACCTTG
    AGTATGGGGA TTGAATACCA TGAGCAGCCC GAGGAGCTGA CCAACATCCT GGAGATCAGC
    AACATCGTCT TCACTAGCAT GTTTGTCCTC GAAATGCTCT TCAAACTGCT GGCCTTTGGC
    ATCTTCGGCT ATATAAGGAA CCCATATAAC ATCTTTGATG GGGTTATTGT TGTCATAAGT
    GTATGGGAGA TCATCGGTCA TGCGGATGGT GGTCTCTCAG TGCTGAGGAC TTTTCGTCTC
    CTGAGGGTGC TCAAACTGGT GCGCTTCCTC CCTGCCCTGC GCAGACAGCT GCTTGTCCTG
    ATGAAGACCA TGGACAATGT GGCAACATTT TGCATGCTAC TCATGCTCTT CATCTTCACT
    TTCAGCATAC TCGGAATGCA TCTGTTCGGA TGCAAATTCA GCCTGAAGAT GGAGAATGGA
    GACACTATTC CGGACAGGAA AAACTTTGAT TCCCTGCTGT GGGCCATTGT CACTGTGTTT
    CAGATTCTGA CCCAGGAAGA CTGGAACGTG GTCCTCTATA ATGGAATGGC TTCCACCAGC
    CCATGGGCGG CTCTTTATTT TGTTGCCCTC ATGACGTTTG GGAATTATGT GCTCTTTAAT
    CTACTGGTGG CCATTTTAGT GGAAGGGTTT CAGGCGGAGG GAGATGCTAA CAAGTCAGAC
    GGAGATGAGG AAAAGACATC AGTGAACTCT GAAGAGAAAA TGGAGCAGCT GCAAAGTTCA
    GACTTGAAGT TCTACTCTCT GATGTTGTCA GCCAATGGGC ATGTGGATCC TTGTGGCACT
    TTGCCTCCAC CAATCATCAT GCGCACTGCT GCCACACCCA TGCCCACTCC AAAGAGCTCT
    CTGGGGCCCG AGTCTGTGTT TGACTTGACC GAGTCCAGAC GGGGCAGTGC TGTCTCCATA
    GATCCAAATG CTTTTGATCA GAAGTCCCTG TCCAGTCCAA GAAGATCACC ATGCCACCCC
    CGTGGCTCGG GCAGTAACTG GGGCAGCCGC AGGTCGTCCT GGAACAGCTT AGGACGAGCG
    CCAAGTCTGA AAAGGAAGGA CACATCTGGG GAGCGCGAGT CTCTGCTGTC CGGGGAAGGC
    AGGGGCAGCT TTGAGGATGA CGACATAGAG GCGGAGAAGC TGACTAACAT CAGCGGAGGC
    TCCCTGCAGC ACCCCAGCTC TCTGGACATC CCAGAACTGC CTCAAATGGG GGAATACCTC
    GACTGCAATG GACGAAGCCA ACACATACTC GCTGACCTGT CTGCCAGTCT CAACAAGGAG
    GACTCCATCG CTGAGGAGGA CTTGGATGAT AGCTTCTGTT TCCGTCTCAG GAGGACCCTG
    GCAGCTTATA AACCCAAGTG GTGCAAAGAT CATGAGGAAT GGTCACTTTA CCTGTTCTCT
    CCTCATAACA AGTTTCGGAT GATGTGTCAA AAACTCATCT CTCACAAGAT GTTTGATTAT
    GTAGTTTTAG TATTCATCTT CCTAAACTGC ATCACTATTG CTCTGGAGAG ACCCCATATT
    CAACAATCTG AGCGATTATT TCTCCTCGTC TCAAACTATG TTTTCACTGT GATATTTGTT
    GCTGAAATGA CAGTGAAGGT TGTGGCCCTT GGTTTCTACT CCGGGAACCA GAGTTATCTA
    AAGAGCACAT GGAATGTTTT GGATGGGGTG CTGGTCTTTG TGTCCCTCAT TGACATTCTG
    GTCTCTCTGG CCTGGACTGG AAACCGAATC TTTGGCATCC TGAGGGTTCT TCGCCTGCTG
    CGAACTCTGC GGCCACTGAG AGTAATTAGT CGTGCTCCTG GTCTGAAGCT GGTAGTAGAA
    ACGTTGATAA CGTCCCTGAG GCCCATTGGG AACATTGTGC TGATCTGCTG TGCTTTCTTT
    ATTGTTTTCG GCATTCTTGG AGTTCAGCTC TTCAAAGGAA AGTTTTTCCA CTGTGAAGGC
    GGCGATACCA GAAACATCAC CAATAAGTCT GACTGTCTTC AGGCAAACCT CAAATGGATC
    AGAAGAAAGT ACAATTTTGA CAACTTGGGA CAGGCTCTCA TGTCTCTTTT CGTGCTTTCC
    TGTAAGGATG GATGGGTCAA TATAATGTAT GATGGGTTGG ATGCTGTTGG TGTGGACCAG
    CAGCCTGAGC GCAACCACAA CCCCTGGATG CTGTTATATT TTATTTCCTT TCTGCTAATC
    GTCAGCTTCT TCGTCCTGAA TATGTTTGTC GGAGTAGTGG TGGAAAACTT CCACAAGTGC
    CGTCAAGACC AGGAGGAAGT GGAGGCGCGT CTGCTGGAGT TGAAGAGGCA AAAACTGATG
    GAGAAAAAGC GCAGGAGTAA GGAGAATTGC GGGTCTGAGG CACTCCGGAG ACCATATTAT
    GCTGACTATT CTCCAGCCCG GCTTTACATC CACACACTGT GCACCAACCA CTATTTGGAC
    CTGTTTATAA CCGGCATCAT CTGCATCAAC GTAGTTACCA TGTCCATCGA GCATTTCAAT
    CAGCCTTCTT ATCTTGACGA GGCACTTAAA TACTGTAACT ATGTATTTAC AATCATCTTC
    ATAATTGAGG CTCTGCTTAA ACTCGTGGCA TTTGGAATAC GGAGATTCTT CAAAGACAGA
    TGGAACCAGC TGGATCTTGC AATAGTCCTA CTCTCTATCA TGGGCATCAC TCTGGAGGAG
    ATTAAGATGA ATGCAGCGCT GCCCATCAAC CCCACCATCA TCCGTATTAT GAGAGTGCTA
    AGAATCGCAA GAGTGCTGAA GCTGTTAAAG ATGGCTACAG GCATGAGATC CCTACTCGAC
    ACCGTAATGC AAGCTTTACC ACAGGTGGGA AATCTGGGCT TGCTGTTTAT GCTTCTCTTC
    TTCATTTATG CGGCGCTTGG CGTGGAGCTT TTTGGGAAAC TAGAATGCAC AGAGAACAAT
    CCGTGTGAAG GATTAAGTCC ACATGCCACG TTTGAGAACT TTGGAATGGC ATTTTTGACA
    CTTTTCCGAG TATCAACTGG GGACAATTGG AATGGAATAA TGAAGGACAC TCTGCGAGAG
    TGCCTTCCTT CAGAGACGCA ATGCCTCAGC TATCTACCCT GGGTCTCTCC AATCTATTTT
    GTAACCTTTG TTCTAATGGC TCAGTTTGTG CTGGTTAACG TGGTCGTAGC TGTGTTGATG
    AAACATCTGG AGGAGAGCAA TAAAGAAGCT AAAGAGGATG CAGAAATGGA TGCTGCGATC
    AAGTTGGCAA TTTTGAAAGA AACTCGCCGT CTCAGCACCA TCTCAGCCAG TGCCACTGCG
    GGACCAGAAG GCAGGGCAAC TTATATCGAG CCACACCCAG ATTCACCTGA GCAGGATGAG
    GGGATGCAAA GAACACAGGG GAATTTGCTC TCTCCGCGGA AAATGTCAGT GTCAAGGATG
    CACTCTTTAC CCAATGACAG CTACATGTTT CGCCCTGTGA GGCCAGCCAG TGCTCCGTAT
    CCTCTAGAAG AAGTGGAGAC CTGTCAAGAT TATCTTGAAT TAGGTTCCAT CACCTCGGTT
    CACTCCCAGC CATGCGAAAG CCTTTCCCTG CTCCAGGTCC CTGGTGTTCC TCCCCATTCC
    CAGTTCAACT GCCTCCCCAA GATCCCTCCT CCCATGCCCT CTCCAACCCA CAGCATAGAC
    AGGATTTTAC GCAGACAGAG GGCAATTAAA AATGACTCCA TTGACATGCA TAATTTCGAC
    TCCAAAGAAA ATGTGGAAAG ACAGAAAGGG ATAACCACAG GCAGCTTCAA GCTGCATGTT
    CCTCAAATCA CCGGCATCTC TCAGGGGTCT CCCTTGCTGC CACCACGTGG ACCTAGTGTC
    CACACCTTCC AGCAGCCGCA CAGCCAGCAC AATATCTCTT CTCGACCTCC CAGTCTGGCC
    AGCAGCAGCA GAAGCACCAG CCCCAGTGGC TCAATGTTTG ACTCCGCTGA CCCAACCGAT
    GAGGAGGTGC GCCACATCAA TAGCACAGCC CGCCTGTGGA TCGGGGTTCA TGCAGAAGCA
    AGGAGTCACT CCCTCTCTCC TTACAGCACC TCAGGGACGC TGCTTCCCAT CCCCGTGAAA
    AACTGCAGCA GTGCTGCCAA CTTAACCATC AAAGACCCCA AGAAGTGCTT CAGTGTGGAC
    ACTGAGGGTT TTCTGGAAAG ACCCTACGCT TCTGATGACC ATCGCAGGCA CTCGATTGAG
    ATTTGCTCCT CAGGAGACGA TGGGCTGTTT GGTCAAGAAC TCTGTGAAAA GAGGCACGTT
    CCCACGCGTG GAAAGTCTTT GCACATTCCC CGGAAGACAA AGATGAGCCC TCCCTGCATC
    TCCATCGAGC CCCCATTTGA AAAGGATGTT TCCCCCTCTC ATGCGTCCAT GAAAAAGTTG
    TCGGATAGCA TGTTACTGCG GAGGAGGACA CCATCCTATG ACCTGGCCCT GCAGCGGGAC
    TCTCTGGACT TGCCGGAGAA CCAGCTGTCA ACCCAGCCAC TCATCAAAGT AGAGCGACAT
    CCCTCTCACT GTGCCGAGTA TCTGTCCCTC CCACACCCCG CCCCAATGAG CGGTCTGGCA
    GGCGTTTTGT GTGAAAGCAC GCAGCCGCCC CCCTTTGATA GCAGATATGA AGAATCCACG
    GACTTCGGCT CTGTAAACAG GACAGCTCAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43368
    Clone ID Related Accession (Same CDS sequence) XM_009299686.2 , XM_009299686.3
    Accession Version XM_009299686.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent T-type calcium channel subunit alpha-1H isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299686.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGCTGGTGA TTCTGCTGAA CTGTGTGACT CTGGGCATGT ATCAGCCATG TGAGGACCTC 
    AAGTGCCAGT CTGAGTGGTG TATCGTCCTG CAGGCGTTTG ATGACTGCAT CTTCGCCTTC
    TTCGCTGTGG AGATGGTCAT CAAAATGATC GCCCTGGGGA TTTTTGGAAT AAATGGTTAT
    CTTGGCGACA CGTGGAACCG CCTGGACTTC TTCATTGTCA TGGCAGGGAT GATGGAGTAT
    TCACTGGACG GTCATAACGC CAGCCTGTCA GCCATCCGGA CTGTGCGAGT GCTCAGGCCC
    CTGCGAGCCA TCAACAGAGT GCCCAGTATG AGGATACTGG TCACTCTGCT TCTAGATACT
    CTGCCTATGT TAGGCAACGT CCTCCTGCTG TGCTTTTTTG TCTTCTTTAT ATTCGGAATT
    GTCGGGGTGC AGCTGTGGGC TGGACTTCTT AGGAATCGGT GTTTCATGCC AAGCAAAGTG
    AGAGACCAAT ATAATCTGTC GTATATGAGT CTGTACTATG AGAACGAAGA TGGTGAAGAC
    AACCCCTTCA TCTGCTCCTC CAGTAAGGAC AACGGCATGA AGCGCTGCAG TGCTGTGCCA
    CCCCTTAAAG AGGGTGTTGA GTGCACTTTA AATGCATCTT TTCTGGGACA TGTCTACTCT
    GGCCTGAGCG GGACAGGCAA CTACAGCTGT GTGAACTGGA ACCAGTATTA CAGTGAATGC
    AAGCCCGGAG ACTTAAACCC GCACAAAGGC GCAGTAAACT TTGATAACAT TGGATATGCC
    TGGATCGCTA TATTCCAGGT TATTACATTG GAAGGATGGG TGGACATCAT GTATTACGTA
    ATGGATGCCC ACTCCTTTTA CAATTTTATA TATTTTATAT TGCTTATTAT AGTGGGTTCC
    TTCTTCATGA TCAACCTGTG CCTGGTTGTA ATAGCAACCC AGTTCGCAGA AACCAAACAG
    CGCGAGAACA TGCTGATGAA GGAGCAGCGT GCACGATACC GCTCCAACGA CAGCACCCTC
    GCCAGCTACT CTGAGCCGGG CAGCTGCTAC GAAGAGATGC TCAAGTACGT CAGCCACCTG
    TACCGCAAGG TGAAGCGCCG CCTCTCCCGC ATCTACAACA GCTGGCAGAG CAAGCGGCGA
    AAGAAAGTGA ACCCCAACAC CGGGGCTGGC GGAGGTGCCA ATGGTCACAG TAGGCACAGG
    GGTTACGGAC ACTGGGTACA GTCCATTCAC AGCCTCATCC AGCAGCACCA GCGACAGCAC
    TGTCACCTCA GCAATGGCAG CCCCAGTCCT GTGGCCACTA CGGGCTCCAG TGAGGCTCTG
    GAAATGAGGT CCATCACAGC GGGTCAGCAG CTGACCGTGT CCTCTCAGCC AACCGCCACG
    CACAATCTTT CTTCCAGCAC ACAATCAGTG CATAGCATTT ATCAAGGTGA CTTTCGTGAG
    GCCCCGCAGG AGCGAACACC CACTAATGTT GCTGTTCCGG CACTCAGCCG ACTCAATGGG
    GGGATGAACT ATCCCACAAT TTTGCCGTCC CTCATTTGCA ACTATTCAGG TGGAGGTTCA
    TTAGGGAAAG AGAGGGGGAA GTCGGATGCA AATACAGGAC ATGAGGTGAT ACAGGACTTT
    GACAAGCTGC ACCAGCAAAT TGAAGCACAT AGCCACAGTC GTGTGCTGCT GCAGATGGCT
    GGTGTGGTGC CCACTCAATT ACTTCTTGAG GTCATGAGCT GCCCGTATTG CGTAAGGGCC
    CTTGAGAGCA TGGATCTGGA GATTGGTGAT TCAGACTACC CTCGATCAGA AGGGGACATG
    GTGTATGAGT TCTCATATGA TGCCGGGTTC ATAACCGGCC GTTCAGCACA TCATAAAATG
    CAGGAGAAAC AAAACCGTCT GGTTCAATTC TGGGAGGACT TCAGGGAAAG ACTGACCCGT
    ATAGTGGATA GCAAATACTT TAACCGGGGA ATTATGATCG CCATCCTCAT CAACACCTTG
    AGTATGGGGA TTGAATACCA TGAGCAGCCC GAGGAGCTGA CCAACATCCT GGAGATCAGC
    AACATCGTCT TCACTAGCAT GTTTGTCCTC GAAATGCTCT TCAAACTGCT GGCCTTTGGC
    ATCTTCGGCT ATATAAGGAA CCCATATAAC ATCTTTGATG GGGTTATTGT TGTCATAAGT
    GTATGGGAGA TCATCGGTCA TGCGGATGGT GGTCTCTCAG TGCTGAGGAC TTTTCGTCTC
    CTGAGGGTGC TCAAACTGGT GCGCTTCCTC CCTGCCCTGC GCAGACAGCT GCTTGTCCTG
    ATGAAGACCA TGGACAATGT GGCAACATTT TGCATGCTAC TCATGCTCTT CATCTTCACT
    TTCAGCATAC TCGGAATGCA TCTGTTCGGA TGCAAATTCA GCCTGAAGAT GGAGAATGGA
    GACACTATTC CGGACAGGAA AAACTTTGAT TCCCTGCTGT GGGCCATTGT CACTGTGTTT
    CAGATTCTGA CCCAGGAAGA CTGGAACGTG GTCCTCTATA ATGGAATGGC TTCCACCAGC
    CCATGGGCGG CTCTTTATTT TGTTGCCCTC ATGACGTTTG GGAATTATGT GCTCTTTAAT
    CTACTGGTGG CCATTTTAGT GGAAGGGTTT CAGGCGGAGG GAGATGCTAA CAAGTCAGAC
    GGAGATGAGG AAAAGACATC AGTGAACTCT GAAGAGAAAA TGGAGCAGCT GCAAAGTTCA
    GCCAATGGGC ATGTGGATCC TTGTGGCACT TTGCCTCCAC CAATCATCAT GCGCACTGCT
    GCCACACCCA TGCCCACTCC AAAGAGCTCT CTGGGGCCCG AGTCTGTGTT TGACTTGACC
    GAGTCCAGAC GGGGCAGTGC TGTCTCCATA GATCCAAATG CTTTTGATCA GAAGTCCCTG
    TCCAGTCCAA GAAGATCACC ATGCCACCCC CGTGGCTCGG GCAGTAACTG GGGCAGCCGC
    AGGTCGTCCT GGAACAGCTT AGGACGAGCG CCAAGTCTGA AAAGGAAGGA CACATCTGGG
    GAGCGCGAGT CTCTGCTGTC CGGGGAAGGC AGGGGCAGCT TTGAGGATGA CGACATAGAG
    GCGGAGAAGC TGACTAACAT CAGCGGAGGC TCCCTGCAGC ACCCCAGCTC TCTGGACATC
    CCAGAACTGC CTCAAATGGG GGAATACCTC GACTGCAATG GACGAAGCCA ACACATACTC
    GCTGACCTGT CTGCCAGTCT CAACAAGGAG GACTCCATCG CTGAGGAGGA CTTGGATGAT
    AGCTTCTGTT TCCGTCTCAG GAGGACCCTG GCAGCTTATA AACCCAAGTG GTGCAAAGAT
    CATGAGGAAT GGTCACTTTA CCTGTTCTCT CCTCATAACA AGTTTCGGAT GATGTGTCAA
    AAACTCATCT CTCACAAGAT GTTTGATTAT GTAGTTTTAG TATTCATCTT CCTAAACTGC
    ATCACTATTG CTCTGGAGAG ACCCCATATT CAACAATCTG AGCGATTATT TCTCCTCGTC
    TCAAACTATG TTTTCACTGT GATATTTGTT GCTGAAATGA CAGTGAAGGT TGTGGCCCTT
    GGTTTCTACT CCGGGAACCA GAGTTATCTA AAGAGCACAT GGAATGTTTT GGATGGGGTG
    CTGGTCTTTG TGTCCCTCAT TGACATTCTG GTCTCTCTGG CCTGGACTGG AAACCGAATC
    TTTGGCATCC TGAGGGTTCT TCGCCTGCTG CGAACTCTGC GGCCACTGAG AGTAATTAGT
    CGTGCTCCTG GTCTGAAGCT GGTAGTAGAA ACGTTGATAA CGTCCCTGAG GCCCATTGGG
    AACATTGTGC TGATCTGCTG TGCTTTCTTT ATTGTTTTCG GCATTCTTGG AGTTCAGCTC
    TTCAAAGGAA AGTTTTTCCA CTGTGAAGGC GGCGATACCA GAAACATCAC CAATAAGTCT
    GACTGTCTTC AGGCAAACCT CAAATGGATC AGAAGAAAGT ACAATTTTGA CAACTTGGGA
    CAGGCTCTCA TGTCTCTTTT CGTGCTTTCC TGTAAGGATG GATGGGTCAA TATAATGTAT
    GATGGGTTGG ATGCTGTTGG TGTGGACCAG CAGCCTGAGC GCAACCACAA CCCCTGGATG
    CTGTTATATT TTATTTCCTT TCTGCTAATC GTCAGCTTCT TCGTCCTGAA TATGTTTGTC
    GGAGTAGTGG TGGAAAACTT CCACAAGTGC CGTCAAGACC AGGAGGAAGT GGAGGCGCGT
    CTGCTGGAGT TGAAGAGGCA AAAACTGATG GAGAAAAAGC GCAGGAGTAA GGAGAATTGC
    GGGTCTGAGG CACTCCGGAG ACCATATTAT GCTGACTATT CTCCAGCCCG GCTTTACATC
    CACACACTGT GCACCAACCA CTATTTGGAC CTGTTTATAA CCGGCATCAT CTGCATCAAC
    GTAGTTACCA TGTCCATCGA GCATTTCAAT CAGCCTTCTT ATCTTGACGA GGCACTTAAA
    TACTGTAACT ATGTATTTAC AATCATCTTC ATAATTGAGG CTCTGCTTAA ACTCGTGGCA
    TTTGGAATAC GGAGATTCTT CAAAGACAGA TGGAACCAGC TGGATCTTGC AATAGTCCTA
    CTCTCTATCA TGGGCATCAC TCTGGAGGAG ATTAAGATGA ATGCAGCGCT GCCCATCAAC
    CCCACCATCA TCCGTATTAT GAGAGTGCTA AGAATCGCAA GAGTGCTGAA GCTGTTAAAG
    ATGGCTACAG GCATGAGATC CCTACTCGAC ACCGTAATGC AAGCTTTACC ACAGGTGGGA
    AATCTGGGCT TGCTGTTTAT GCTTCTCTTC TTCATTTATG CGGCGCTTGG CGTGGAGCTT
    TTTGGGAAAC TAGAATGCAC AGAGAACAAT CCGTGTGAAG GATTAAGTCC ACATGCCACG
    TTTGAGAACT TTGGAATGGC ATTTTTGACA CTTTTCCGAG TATCAACTGG GGACAATTGG
    AATGGAATAA TGAAGGACAC TCTGCGAGAG TGCCTTCCTT CAGAGACGCA ATGCCTCAGC
    TATCTACCCT GGGTCTCTCC AATCTATTTT GTAACCTTTG TTCTAATGGC TCAGTTTGTG
    CTGGTTAACG TGGTCGTAGC TGTGTTGATG AAACATCTGG AGGAGAGCAA TAAAGAAGCT
    AAAGAGGATG CAGAAATGGA TGCTGCGATC AAGTTGGCAA TTTTGAAAGA AACTCGCCGT
    CTCAGCACCA TCTCAGCCAG TGCCACTGCG GGACCAGAAG GCAGGGCAAC TTATATCGAG
    CCACACCCAG ATTCACCTGA GCAGGATGAG GGGATGCAAA GAACACAGGG GAATTTGCTC
    TCTCCGCGGA AAATGTCAGT GTCAAGGATG CACTCTTTAC CCAATGACAG CTACATGTTT
    CGCCCTGTGA GGCCAGCCAG TGCTCCGTAT CCTCTAGAAG AAGTGGAGAC CTGTCAAGAT
    TATCTTGAAT TAGGTTCCAT CACCTCGGTT CACTCCCAGC CATGCGAAAG CCTTTCCCTG
    CTCCAGGTCC CTGGTGTTCC TCCCCATTCC CAGTTCAACT GCCTCCCCAA GATCCCTCCT
    CCCATGCCCT CTCCAACCCA CAGCATAGAC AGGATTTTAC GCAGACAGAG GGCAATTAAA
    AATGACTCCA TTGACATGCA TAATTTCGAC TCCAAAGAAA ATGTGGAAAG ACAGAAAGGG
    ATAACCACAG GCAGCTTCAA GCTGCATGTT CCTCAAATCA CCGGCATCTC TCAGGGGTCT
    CCCTTGCTGC CACCACGTGG ACCTAGTGTC CACACCTTCC AGCAGCCGCA CAGCCAGCAC
    AATATCTCTT CTCGACCTCC CAGTCTGGCC AGCAGCAGCA GAAGCACCAG CCCCAGTGGC
    TCAATGTTTG ACTCCGCTGA CCCAACCGAT GAGGAGGTGC GCCACATCAA TAGCACAGCC
    CGCCTGTGGA TCGGGGTTCA TGCAGAAGCA AGGAGTCACT CCCTCTCTCC TTACAGCACC
    TCAGGGACGC TGCTTCCCAT CCCCGTGAAA AACTGCAGCA GTGCTGCCAA CTTAACCATC
    AAAGACCCCA AGAAGTGCTT CAGTGTGGAC ACTGAGGGTT TTCTGGAAAG ACCCTACGCT
    TCTGATGACC ATCGCAGGCA CTCGATTGAG ATTTGCTCCT CAGGAGACGA TGGGCTGTTT
    GGTCAAGAAC TCTGTGAAAA GAGGCACGTT CCCACGCGTG GAAAGTCTTT GCACATTCCC
    CGGAAGACAA AGATGAGCCC TCCCTGCATC TCCATCGAGC CCCCATTTGA AAAGGATGTT
    TCCCCCTCTC ATGCGTCCAT GAAAAAGTTG TCGGATAGCA TGTTACTGCG GAGGAGGACA
    CCATCCTATG ACCTGGCCCT GCAGCGGGAC TCTCTGGACT TGCCGGAGAA CCAGCTGTCA
    ACCCAGCCAC TCATCAAAGT AGAGCGACAT CCCTCTCACT GTGCCGAGTA TCTGTCCCTC
    CCACACCCCG CCCCAATGAG CGGTCTGGCA GGCGTTTTGT GTGAAAGCAC GCAGCCGCCC
    CCCTTTGATA GCAGATATGA AGAATCCACG GACTTCGGCT CTGTAAACAG GACAGCTCAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297961.1
    CDS19..6561
    Translation

    Target ORF information:

    RefSeq Version XM_009299686.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, T type, alpha 1H subunit a (cacna1ha), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299686.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGCTGGTGA TTCTGCTGAA CTGTGTGACT CTGGGCATGT ATCAGCCATG TGAGGACCTC 
    AAGTGCCAGT CTGAGTGGTG TATCGTCCTG CAGGCGTTTG ATGACTGCAT CTTCGCCTTC
    TTCGCTGTGG AGATGGTCAT CAAAATGATC GCCCTGGGGA TTTTTGGAAT AAATGGTTAT
    CTTGGCGACA CGTGGAACCG CCTGGACTTC TTCATTGTCA TGGCAGGGAT GATGGAGTAT
    TCACTGGACG GTCATAACGC CAGCCTGTCA GCCATCCGGA CTGTGCGAGT GCTCAGGCCC
    CTGCGAGCCA TCAACAGAGT GCCCAGTATG AGGATACTGG TCACTCTGCT TCTAGATACT
    CTGCCTATGT TAGGCAACGT CCTCCTGCTG TGCTTTTTTG TCTTCTTTAT ATTCGGAATT
    GTCGGGGTGC AGCTGTGGGC TGGACTTCTT AGGAATCGGT GTTTCATGCC AAGCAAAGTG
    AGAGACCAAT ATAATCTGTC GTATATGAGT CTGTACTATG AGAACGAAGA TGGTGAAGAC
    AACCCCTTCA TCTGCTCCTC CAGTAAGGAC AACGGCATGA AGCGCTGCAG TGCTGTGCCA
    CCCCTTAAAG AGGGTGTTGA GTGCACTTTA AATGCATCTT TTCTGGGACA TGTCTACTCT
    GGCCTGAGCG GGACAGGCAA CTACAGCTGT GTGAACTGGA ACCAGTATTA CAGTGAATGC
    AAGCCCGGAG ACTTAAACCC GCACAAAGGC GCAGTAAACT TTGATAACAT TGGATATGCC
    TGGATCGCTA TATTCCAGGT TATTACATTG GAAGGATGGG TGGACATCAT GTATTACGTA
    ATGGATGCCC ACTCCTTTTA CAATTTTATA TATTTTATAT TGCTTATTAT AGTGGGTTCC
    TTCTTCATGA TCAACCTGTG CCTGGTTGTA ATAGCAACCC AGTTCGCAGA AACCAAACAG
    CGCGAGAACA TGCTGATGAA GGAGCAGCGT GCACGATACC GCTCCAACGA CAGCACCCTC
    GCCAGCTACT CTGAGCCGGG CAGCTGCTAC GAAGAGATGC TCAAGTACGT CAGCCACCTG
    TACCGCAAGG TGAAGCGCCG CCTCTCCCGC ATCTACAACA GCTGGCAGAG CAAGCGGCGA
    AAGAAAGTGA ACCCCAACAC CGGGGCTGGC GGAGGTGCCA ATGGTCACAG TAGGCACAGG
    GGTTACGGAC ACTGGGTACA GTCCATTCAC AGCCTCATCC AGCAGCACCA GCGACAGCAC
    TGTCACCTCA GCAATGGCAG CCCCAGTCCT GTGGCCACTA CGGGCTCCAG TGAGGCTCTG
    GAAATGAGGT CCATCACAGC GGGTCAGCAG CTGACCGTGT CCTCTCAGCC AACCGCCACG
    CACAATCTTT CTTCCAGCAC ACAATCAGTG CATAGCATTT ATCAAGGTGA CTTTCGTGAG
    GCCCCGCAGG AGCGAACACC CACTAATGTT GCTGTTCCGG CACTCAGCCG ACTCAATGGG
    GGGATGAACT ATCCCACAAT TTTGCCGTCC CTCATTTGCA ACTATTCAGG TGGAGGTTCA
    TTAGGGAAAG AGAGGGGGAA GTCGGATGCA AATACAGGAC ATGAGGTGAT ACAGGACTTT
    GACAAGCTGC ACCAGCAAAT TGAAGCACAT AGCCACAGTC GTGTGCTGCT GCAGATGGCT
    GGTGTGGTGC CCACTCAATT ACTTCTTGAG GTCATGAGCT GCCCGTATTG CGTAAGGGCC
    CTTGAGAGCA TGGATCTGGA GATTGGTGAT TCAGACTACC CTCGATCAGA AGGGGACATG
    GTGTATGAGT TCTCATATGA TGCCGGGTTC ATAACCGGCC GTTCAGCACA TCATAAAATG
    CAGGAGAAAC AAAACCGTCT GGTTCAATTC TGGGAGGACT TCAGGGAAAG ACTGACCCGT
    ATAGTGGATA GCAAATACTT TAACCGGGGA ATTATGATCG CCATCCTCAT CAACACCTTG
    AGTATGGGGA TTGAATACCA TGAGCAGCCC GAGGAGCTGA CCAACATCCT GGAGATCAGC
    AACATCGTCT TCACTAGCAT GTTTGTCCTC GAAATGCTCT TCAAACTGCT GGCCTTTGGC
    ATCTTCGGCT ATATAAGGAA CCCATATAAC ATCTTTGATG GGGTTATTGT TGTCATAAGT
    GTATGGGAGA TCATCGGTCA TGCGGATGGT GGTCTCTCAG TGCTGAGGAC TTTTCGTCTC
    CTGAGGGTGC TCAAACTGGT GCGCTTCCTC CCTGCCCTGC GCAGACAGCT GCTTGTCCTG
    ATGAAGACCA TGGACAATGT GGCAACATTT TGCATGCTAC TCATGCTCTT CATCTTCACT
    TTCAGCATAC TCGGAATGCA TCTGTTCGGA TGCAAATTCA GCCTGAAGAT GGAGAATGGA
    GACACTATTC CGGACAGGAA AAACTTTGAT TCCCTGCTGT GGGCCATTGT CACTGTGTTT
    CAGATTCTGA CCCAGGAAGA CTGGAACGTG GTCCTCTATA ATGGAATGGC TTCCACCAGC
    CCATGGGCGG CTCTTTATTT TGTTGCCCTC ATGACGTTTG GGAATTATGT GCTCTTTAAT
    CTACTGGTGG CCATTTTAGT GGAAGGGTTT CAGGCGGAGG GAGATGCTAA CAAGTCAGAC
    GGAGATGAGG AAAAGACATC AGTGAACTCT GAAGAGAAAA TGGAGCAGCT GCAAAGTTCA
    GCCAATGGGC ATGTGGATCC TTGTGGCACT TTGCCTCCAC CAATCATCAT GCGCACTGCT
    GCCACACCCA TGCCCACTCC AAAGAGCTCT CTGGGGCCCG AGTCTGTGTT TGACTTGACC
    GAGTCCAGAC GGGGCAGTGC TGTCTCCATA GATCCAAATG CTTTTGATCA GAAGTCCCTG
    TCCAGTCCAA GAAGATCACC ATGCCACCCC CGTGGCTCGG GCAGTAACTG GGGCAGCCGC
    AGGTCGTCCT GGAACAGCTT AGGACGAGCG CCAAGTCTGA AAAGGAAGGA CACATCTGGG
    GAGCGCGAGT CTCTGCTGTC CGGGGAAGGC AGGGGCAGCT TTGAGGATGA CGACATAGAG
    GCGGAGAAGC TGACTAACAT CAGCGGAGGC TCCCTGCAGC ACCCCAGCTC TCTGGACATC
    CCAGAACTGC CTCAAATGGG GGAATACCTC GACTGCAATG GACGAAGCCA ACACATACTC
    GCTGACCTGT CTGCCAGTCT CAACAAGGAG GACTCCATCG CTGAGGAGGA CTTGGATGAT
    AGCTTCTGTT TCCGTCTCAG GAGGACCCTG GCAGCTTATA AACCCAAGTG GTGCAAAGAT
    CATGAGGAAT GGTCACTTTA CCTGTTCTCT CCTCATAACA AGTTTCGGAT GATGTGTCAA
    AAACTCATCT CTCACAAGAT GTTTGATTAT GTAGTTTTAG TATTCATCTT CCTAAACTGC
    ATCACTATTG CTCTGGAGAG ACCCCATATT CAACAATCTG AGCGATTATT TCTCCTCGTC
    TCAAACTATG TTTTCACTGT GATATTTGTT GCTGAAATGA CAGTGAAGGT TGTGGCCCTT
    GGTTTCTACT CCGGGAACCA GAGTTATCTA AAGAGCACAT GGAATGTTTT GGATGGGGTG
    CTGGTCTTTG TGTCCCTCAT TGACATTCTG GTCTCTCTGG CCTGGACTGG AAACCGAATC
    TTTGGCATCC TGAGGGTTCT TCGCCTGCTG CGAACTCTGC GGCCACTGAG AGTAATTAGT
    CGTGCTCCTG GTCTGAAGCT GGTAGTAGAA ACGTTGATAA CGTCCCTGAG GCCCATTGGG
    AACATTGTGC TGATCTGCTG TGCTTTCTTT ATTGTTTTCG GCATTCTTGG AGTTCAGCTC
    TTCAAAGGAA AGTTTTTCCA CTGTGAAGGC GGCGATACCA GAAACATCAC CAATAAGTCT
    GACTGTCTTC AGGCAAACCT CAAATGGATC AGAAGAAAGT ACAATTTTGA CAACTTGGGA
    CAGGCTCTCA TGTCTCTTTT CGTGCTTTCC TGTAAGGATG GATGGGTCAA TATAATGTAT
    GATGGGTTGG ATGCTGTTGG TGTGGACCAG CAGCCTGAGC GCAACCACAA CCCCTGGATG
    CTGTTATATT TTATTTCCTT TCTGCTAATC GTCAGCTTCT TCGTCCTGAA TATGTTTGTC
    GGAGTAGTGG TGGAAAACTT CCACAAGTGC CGTCAAGACC AGGAGGAAGT GGAGGCGCGT
    CTGCTGGAGT TGAAGAGGCA AAAACTGATG GAGAAAAAGC GCAGGAGTAA GGAGAATTGC
    GGGTCTGAGG CACTCCGGAG ACCATATTAT GCTGACTATT CTCCAGCCCG GCTTTACATC
    CACACACTGT GCACCAACCA CTATTTGGAC CTGTTTATAA CCGGCATCAT CTGCATCAAC
    GTAGTTACCA TGTCCATCGA GCATTTCAAT CAGCCTTCTT ATCTTGACGA GGCACTTAAA
    TACTGTAACT ATGTATTTAC AATCATCTTC ATAATTGAGG CTCTGCTTAA ACTCGTGGCA
    TTTGGAATAC GGAGATTCTT CAAAGACAGA TGGAACCAGC TGGATCTTGC AATAGTCCTA
    CTCTCTATCA TGGGCATCAC TCTGGAGGAG ATTAAGATGA ATGCAGCGCT GCCCATCAAC
    CCCACCATCA TCCGTATTAT GAGAGTGCTA AGAATCGCAA GAGTGCTGAA GCTGTTAAAG
    ATGGCTACAG GCATGAGATC CCTACTCGAC ACCGTAATGC AAGCTTTACC ACAGGTGGGA
    AATCTGGGCT TGCTGTTTAT GCTTCTCTTC TTCATTTATG CGGCGCTTGG CGTGGAGCTT
    TTTGGGAAAC TAGAATGCAC AGAGAACAAT CCGTGTGAAG GATTAAGTCC ACATGCCACG
    TTTGAGAACT TTGGAATGGC ATTTTTGACA CTTTTCCGAG TATCAACTGG GGACAATTGG
    AATGGAATAA TGAAGGACAC TCTGCGAGAG TGCCTTCCTT CAGAGACGCA ATGCCTCAGC
    TATCTACCCT GGGTCTCTCC AATCTATTTT GTAACCTTTG TTCTAATGGC TCAGTTTGTG
    CTGGTTAACG TGGTCGTAGC TGTGTTGATG AAACATCTGG AGGAGAGCAA TAAAGAAGCT
    AAAGAGGATG CAGAAATGGA TGCTGCGATC AAGTTGGCAA TTTTGAAAGA AACTCGCCGT
    CTCAGCACCA TCTCAGCCAG TGCCACTGCG GGACCAGAAG GCAGGGCAAC TTATATCGAG
    CCACACCCAG ATTCACCTGA GCAGGATGAG GGGATGCAAA GAACACAGGG GAATTTGCTC
    TCTCCGCGGA AAATGTCAGT GTCAAGGATG CACTCTTTAC CCAATGACAG CTACATGTTT
    CGCCCTGTGA GGCCAGCCAG TGCTCCGTAT CCTCTAGAAG AAGTGGAGAC CTGTCAAGAT
    TATCTTGAAT TAGGTTCCAT CACCTCGGTT CACTCCCAGC CATGCGAAAG CCTTTCCCTG
    CTCCAGGTCC CTGGTGTTCC TCCCCATTCC CAGTTCAACT GCCTCCCCAA GATCCCTCCT
    CCCATGCCCT CTCCAACCCA CAGCATAGAC AGGATTTTAC GCAGACAGAG GGCAATTAAA
    AATGACTCCA TTGACATGCA TAATTTCGAC TCCAAAGAAA ATGTGGAAAG ACAGAAAGGG
    ATAACCACAG GCAGCTTCAA GCTGCATGTT CCTCAAATCA CCGGCATCTC TCAGGGGTCT
    CCCTTGCTGC CACCACGTGG ACCTAGTGTC CACACCTTCC AGCAGCCGCA CAGCCAGCAC
    AATATCTCTT CTCGACCTCC CAGTCTGGCC AGCAGCAGCA GAAGCACCAG CCCCAGTGGC
    TCAATGTTTG ACTCCGCTGA CCCAACCGAT GAGGAGGTGC GCCACATCAA TAGCACAGCC
    CGCCTGTGGA TCGGGGTTCA TGCAGAAGCA AGGAGTCACT CCCTCTCTCC TTACAGCACC
    TCAGGGACGC TGCTTCCCAT CCCCGTGAAA AACTGCAGCA GTGCTGCCAA CTTAACCATC
    AAAGACCCCA AGAAGTGCTT CAGTGTGGAC ACTGAGGGTT TTCTGGAAAG ACCCTACGCT
    TCTGATGACC ATCGCAGGCA CTCGATTGAG ATTTGCTCCT CAGGAGACGA TGGGCTGTTT
    GGTCAAGAAC TCTGTGAAAA GAGGCACGTT CCCACGCGTG GAAAGTCTTT GCACATTCCC
    CGGAAGACAA AGATGAGCCC TCCCTGCATC TCCATCGAGC CCCCATTTGA AAAGGATGTT
    TCCCCCTCTC ATGCGTCCAT GAAAAAGTTG TCGGATAGCA TGTTACTGCG GAGGAGGACA
    CCATCCTATG ACCTGGCCCT GCAGCGGGAC TCTCTGGACT TGCCGGAGAA CCAGCTGTCA
    ACCCAGCCAC TCATCAAAGT AGAGCGACAT CCCTCTCACT GTGCCGAGTA TCTGTCCCTC
    CCACACCCCG CCCCAATGAG CGGTCTGGCA GGCGTTTTGT GTGAAAGCAC GCAGCCGCCC
    CCCTTTGATA GCAGATATGA AGAATCCACG GACTTCGGCT CTGTAAACAG GACAGCTCAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43368
    Clone ID Related Accession (Same CDS sequence) XM_009299686.2 , XM_009299686.3
    Accession Version XM_009299686.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent T-type calcium channel subunit alpha-1H isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299686.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGCTGGTGA TTCTGCTGAA CTGTGTGACT CTGGGCATGT ATCAGCCATG TGAGGACCTC 
    AAGTGCCAGT CTGAGTGGTG TATCGTCCTG CAGGCGTTTG ATGACTGCAT CTTCGCCTTC
    TTCGCTGTGG AGATGGTCAT CAAAATGATC GCCCTGGGGA TTTTTGGAAT AAATGGTTAT
    CTTGGCGACA CGTGGAACCG CCTGGACTTC TTCATTGTCA TGGCAGGGAT GATGGAGTAT
    TCACTGGACG GTCATAACGC CAGCCTGTCA GCCATCCGGA CTGTGCGAGT GCTCAGGCCC
    CTGCGAGCCA TCAACAGAGT GCCCAGTATG AGGATACTGG TCACTCTGCT TCTAGATACT
    CTGCCTATGT TAGGCAACGT CCTCCTGCTG TGCTTTTTTG TCTTCTTTAT ATTCGGAATT
    GTCGGGGTGC AGCTGTGGGC TGGACTTCTT AGGAATCGGT GTTTCATGCC AAGCAAAGTG
    AGAGACCAAT ATAATCTGTC GTATATGAGT CTGTACTATG AGAACGAAGA TGGTGAAGAC
    AACCCCTTCA TCTGCTCCTC CAGTAAGGAC AACGGCATGA AGCGCTGCAG TGCTGTGCCA
    CCCCTTAAAG AGGGTGTTGA GTGCACTTTA AATGCATCTT TTCTGGGACA TGTCTACTCT
    GGCCTGAGCG GGACAGGCAA CTACAGCTGT GTGAACTGGA ACCAGTATTA CAGTGAATGC
    AAGCCCGGAG ACTTAAACCC GCACAAAGGC GCAGTAAACT TTGATAACAT TGGATATGCC
    TGGATCGCTA TATTCCAGGT TATTACATTG GAAGGATGGG TGGACATCAT GTATTACGTA
    ATGGATGCCC ACTCCTTTTA CAATTTTATA TATTTTATAT TGCTTATTAT AGTGGGTTCC
    TTCTTCATGA TCAACCTGTG CCTGGTTGTA ATAGCAACCC AGTTCGCAGA AACCAAACAG
    CGCGAGAACA TGCTGATGAA GGAGCAGCGT GCACGATACC GCTCCAACGA CAGCACCCTC
    GCCAGCTACT CTGAGCCGGG CAGCTGCTAC GAAGAGATGC TCAAGTACGT CAGCCACCTG
    TACCGCAAGG TGAAGCGCCG CCTCTCCCGC ATCTACAACA GCTGGCAGAG CAAGCGGCGA
    AAGAAAGTGA ACCCCAACAC CGGGGCTGGC GGAGGTGCCA ATGGTCACAG TAGGCACAGG
    GGTTACGGAC ACTGGGTACA GTCCATTCAC AGCCTCATCC AGCAGCACCA GCGACAGCAC
    TGTCACCTCA GCAATGGCAG CCCCAGTCCT GTGGCCACTA CGGGCTCCAG TGAGGCTCTG
    GAAATGAGGT CCATCACAGC GGGTCAGCAG CTGACCGTGT CCTCTCAGCC AACCGCCACG
    CACAATCTTT CTTCCAGCAC ACAATCAGTG CATAGCATTT ATCAAGGTGA CTTTCGTGAG
    GCCCCGCAGG AGCGAACACC CACTAATGTT GCTGTTCCGG CACTCAGCCG ACTCAATGGG
    GGGATGAACT ATCCCACAAT TTTGCCGTCC CTCATTTGCA ACTATTCAGG TGGAGGTTCA
    TTAGGGAAAG AGAGGGGGAA GTCGGATGCA AATACAGGAC ATGAGGTGAT ACAGGACTTT
    GACAAGCTGC ACCAGCAAAT TGAAGCACAT AGCCACAGTC GTGTGCTGCT GCAGATGGCT
    GGTGTGGTGC CCACTCAATT ACTTCTTGAG GTCATGAGCT GCCCGTATTG CGTAAGGGCC
    CTTGAGAGCA TGGATCTGGA GATTGGTGAT TCAGACTACC CTCGATCAGA AGGGGACATG
    GTGTATGAGT TCTCATATGA TGCCGGGTTC ATAACCGGCC GTTCAGCACA TCATAAAATG
    CAGGAGAAAC AAAACCGTCT GGTTCAATTC TGGGAGGACT TCAGGGAAAG ACTGACCCGT
    ATAGTGGATA GCAAATACTT TAACCGGGGA ATTATGATCG CCATCCTCAT CAACACCTTG
    AGTATGGGGA TTGAATACCA TGAGCAGCCC GAGGAGCTGA CCAACATCCT GGAGATCAGC
    AACATCGTCT TCACTAGCAT GTTTGTCCTC GAAATGCTCT TCAAACTGCT GGCCTTTGGC
    ATCTTCGGCT ATATAAGGAA CCCATATAAC ATCTTTGATG GGGTTATTGT TGTCATAAGT
    GTATGGGAGA TCATCGGTCA TGCGGATGGT GGTCTCTCAG TGCTGAGGAC TTTTCGTCTC
    CTGAGGGTGC TCAAACTGGT GCGCTTCCTC CCTGCCCTGC GCAGACAGCT GCTTGTCCTG
    ATGAAGACCA TGGACAATGT GGCAACATTT TGCATGCTAC TCATGCTCTT CATCTTCACT
    TTCAGCATAC TCGGAATGCA TCTGTTCGGA TGCAAATTCA GCCTGAAGAT GGAGAATGGA
    GACACTATTC CGGACAGGAA AAACTTTGAT TCCCTGCTGT GGGCCATTGT CACTGTGTTT
    CAGATTCTGA CCCAGGAAGA CTGGAACGTG GTCCTCTATA ATGGAATGGC TTCCACCAGC
    CCATGGGCGG CTCTTTATTT TGTTGCCCTC ATGACGTTTG GGAATTATGT GCTCTTTAAT
    CTACTGGTGG CCATTTTAGT GGAAGGGTTT CAGGCGGAGG GAGATGCTAA CAAGTCAGAC
    GGAGATGAGG AAAAGACATC AGTGAACTCT GAAGAGAAAA TGGAGCAGCT GCAAAGTTCA
    GCCAATGGGC ATGTGGATCC TTGTGGCACT TTGCCTCCAC CAATCATCAT GCGCACTGCT
    GCCACACCCA TGCCCACTCC AAAGAGCTCT CTGGGGCCCG AGTCTGTGTT TGACTTGACC
    GAGTCCAGAC GGGGCAGTGC TGTCTCCATA GATCCAAATG CTTTTGATCA GAAGTCCCTG
    TCCAGTCCAA GAAGATCACC ATGCCACCCC CGTGGCTCGG GCAGTAACTG GGGCAGCCGC
    AGGTCGTCCT GGAACAGCTT AGGACGAGCG CCAAGTCTGA AAAGGAAGGA CACATCTGGG
    GAGCGCGAGT CTCTGCTGTC CGGGGAAGGC AGGGGCAGCT TTGAGGATGA CGACATAGAG
    GCGGAGAAGC TGACTAACAT CAGCGGAGGC TCCCTGCAGC ACCCCAGCTC TCTGGACATC
    CCAGAACTGC CTCAAATGGG GGAATACCTC GACTGCAATG GACGAAGCCA ACACATACTC
    GCTGACCTGT CTGCCAGTCT CAACAAGGAG GACTCCATCG CTGAGGAGGA CTTGGATGAT
    AGCTTCTGTT TCCGTCTCAG GAGGACCCTG GCAGCTTATA AACCCAAGTG GTGCAAAGAT
    CATGAGGAAT GGTCACTTTA CCTGTTCTCT CCTCATAACA AGTTTCGGAT GATGTGTCAA
    AAACTCATCT CTCACAAGAT GTTTGATTAT GTAGTTTTAG TATTCATCTT CCTAAACTGC
    ATCACTATTG CTCTGGAGAG ACCCCATATT CAACAATCTG AGCGATTATT TCTCCTCGTC
    TCAAACTATG TTTTCACTGT GATATTTGTT GCTGAAATGA CAGTGAAGGT TGTGGCCCTT
    GGTTTCTACT CCGGGAACCA GAGTTATCTA AAGAGCACAT GGAATGTTTT GGATGGGGTG
    CTGGTCTTTG TGTCCCTCAT TGACATTCTG GTCTCTCTGG CCTGGACTGG AAACCGAATC
    TTTGGCATCC TGAGGGTTCT TCGCCTGCTG CGAACTCTGC GGCCACTGAG AGTAATTAGT
    CGTGCTCCTG GTCTGAAGCT GGTAGTAGAA ACGTTGATAA CGTCCCTGAG GCCCATTGGG
    AACATTGTGC TGATCTGCTG TGCTTTCTTT ATTGTTTTCG GCATTCTTGG AGTTCAGCTC
    TTCAAAGGAA AGTTTTTCCA CTGTGAAGGC GGCGATACCA GAAACATCAC CAATAAGTCT
    GACTGTCTTC AGGCAAACCT CAAATGGATC AGAAGAAAGT ACAATTTTGA CAACTTGGGA
    CAGGCTCTCA TGTCTCTTTT CGTGCTTTCC TGTAAGGATG GATGGGTCAA TATAATGTAT
    GATGGGTTGG ATGCTGTTGG TGTGGACCAG CAGCCTGAGC GCAACCACAA CCCCTGGATG
    CTGTTATATT TTATTTCCTT TCTGCTAATC GTCAGCTTCT TCGTCCTGAA TATGTTTGTC
    GGAGTAGTGG TGGAAAACTT CCACAAGTGC CGTCAAGACC AGGAGGAAGT GGAGGCGCGT
    CTGCTGGAGT TGAAGAGGCA AAAACTGATG GAGAAAAAGC GCAGGAGTAA GGAGAATTGC
    GGGTCTGAGG CACTCCGGAG ACCATATTAT GCTGACTATT CTCCAGCCCG GCTTTACATC
    CACACACTGT GCACCAACCA CTATTTGGAC CTGTTTATAA CCGGCATCAT CTGCATCAAC
    GTAGTTACCA TGTCCATCGA GCATTTCAAT CAGCCTTCTT ATCTTGACGA GGCACTTAAA
    TACTGTAACT ATGTATTTAC AATCATCTTC ATAATTGAGG CTCTGCTTAA ACTCGTGGCA
    TTTGGAATAC GGAGATTCTT CAAAGACAGA TGGAACCAGC TGGATCTTGC AATAGTCCTA
    CTCTCTATCA TGGGCATCAC TCTGGAGGAG ATTAAGATGA ATGCAGCGCT GCCCATCAAC
    CCCACCATCA TCCGTATTAT GAGAGTGCTA AGAATCGCAA GAGTGCTGAA GCTGTTAAAG
    ATGGCTACAG GCATGAGATC CCTACTCGAC ACCGTAATGC AAGCTTTACC ACAGGTGGGA
    AATCTGGGCT TGCTGTTTAT GCTTCTCTTC TTCATTTATG CGGCGCTTGG CGTGGAGCTT
    TTTGGGAAAC TAGAATGCAC AGAGAACAAT CCGTGTGAAG GATTAAGTCC ACATGCCACG
    TTTGAGAACT TTGGAATGGC ATTTTTGACA CTTTTCCGAG TATCAACTGG GGACAATTGG
    AATGGAATAA TGAAGGACAC TCTGCGAGAG TGCCTTCCTT CAGAGACGCA ATGCCTCAGC
    TATCTACCCT GGGTCTCTCC AATCTATTTT GTAACCTTTG TTCTAATGGC TCAGTTTGTG
    CTGGTTAACG TGGTCGTAGC TGTGTTGATG AAACATCTGG AGGAGAGCAA TAAAGAAGCT
    AAAGAGGATG CAGAAATGGA TGCTGCGATC AAGTTGGCAA TTTTGAAAGA AACTCGCCGT
    CTCAGCACCA TCTCAGCCAG TGCCACTGCG GGACCAGAAG GCAGGGCAAC TTATATCGAG
    CCACACCCAG ATTCACCTGA GCAGGATGAG GGGATGCAAA GAACACAGGG GAATTTGCTC
    TCTCCGCGGA AAATGTCAGT GTCAAGGATG CACTCTTTAC CCAATGACAG CTACATGTTT
    CGCCCTGTGA GGCCAGCCAG TGCTCCGTAT CCTCTAGAAG AAGTGGAGAC CTGTCAAGAT
    TATCTTGAAT TAGGTTCCAT CACCTCGGTT CACTCCCAGC CATGCGAAAG CCTTTCCCTG
    CTCCAGGTCC CTGGTGTTCC TCCCCATTCC CAGTTCAACT GCCTCCCCAA GATCCCTCCT
    CCCATGCCCT CTCCAACCCA CAGCATAGAC AGGATTTTAC GCAGACAGAG GGCAATTAAA
    AATGACTCCA TTGACATGCA TAATTTCGAC TCCAAAGAAA ATGTGGAAAG ACAGAAAGGG
    ATAACCACAG GCAGCTTCAA GCTGCATGTT CCTCAAATCA CCGGCATCTC TCAGGGGTCT
    CCCTTGCTGC CACCACGTGG ACCTAGTGTC CACACCTTCC AGCAGCCGCA CAGCCAGCAC
    AATATCTCTT CTCGACCTCC CAGTCTGGCC AGCAGCAGCA GAAGCACCAG CCCCAGTGGC
    TCAATGTTTG ACTCCGCTGA CCCAACCGAT GAGGAGGTGC GCCACATCAA TAGCACAGCC
    CGCCTGTGGA TCGGGGTTCA TGCAGAAGCA AGGAGTCACT CCCTCTCTCC TTACAGCACC
    TCAGGGACGC TGCTTCCCAT CCCCGTGAAA AACTGCAGCA GTGCTGCCAA CTTAACCATC
    AAAGACCCCA AGAAGTGCTT CAGTGTGGAC ACTGAGGGTT TTCTGGAAAG ACCCTACGCT
    TCTGATGACC ATCGCAGGCA CTCGATTGAG ATTTGCTCCT CAGGAGACGA TGGGCTGTTT
    GGTCAAGAAC TCTGTGAAAA GAGGCACGTT CCCACGCGTG GAAAGTCTTT GCACATTCCC
    CGGAAGACAA AGATGAGCCC TCCCTGCATC TCCATCGAGC CCCCATTTGA AAAGGATGTT
    TCCCCCTCTC ATGCGTCCAT GAAAAAGTTG TCGGATAGCA TGTTACTGCG GAGGAGGACA
    CCATCCTATG ACCTGGCCCT GCAGCGGGAC TCTCTGGACT TGCCGGAGAA CCAGCTGTCA
    ACCCAGCCAC TCATCAAAGT AGAGCGACAT CCCTCTCACT GTGCCGAGTA TCTGTCCCTC
    CCACACCCCG CCCCAATGAG CGGTCTGGCA GGCGTTTTGT GTGAAAGCAC GCAGCCGCCC
    CCCTTTGATA GCAGATATGA AGAATCCACG GACTTCGGCT CTGTAAACAG GACAGCTCAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297961.1
    CDS484..7026
    Translation

    Target ORF information:

    RefSeq Version XM_009299686.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, T type, alpha 1H subunit a (cacna1ha), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299686.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGCTGGTGA TTCTGCTGAA CTGTGTGACT CTGGGCATGT ATCAGCCATG TGAGGACCTC 
    AAGTGCCAGT CTGAGTGGTG TATCGTCCTG CAGGCGTTTG ATGACTGCAT CTTCGCCTTC
    TTCGCTGTGG AGATGGTCAT CAAAATGATC GCCCTGGGGA TTTTTGGAAT AAATGGTTAT
    CTTGGCGACA CGTGGAACCG CCTGGACTTC TTCATTGTCA TGGCAGGGAT GATGGAGTAT
    TCACTGGACG GTCATAACGC CAGCCTGTCA GCCATCCGGA CTGTGCGAGT GCTCAGGCCC
    CTGCGAGCCA TCAACAGAGT GCCCAGTATG AGGATACTGG TCACTCTGCT TCTAGATACT
    CTGCCTATGT TAGGCAACGT CCTCCTGCTG TGCTTTTTTG TCTTCTTTAT ATTCGGAATT
    GTCGGGGTGC AGCTGTGGGC TGGACTTCTT AGGAATCGGT GTTTCATGCC AAGCAAAGTG
    AGAGACCAAT ATAATCTGTC GTATATGAGT CTGTACTATG AGAACGAAGA TGGTGAAGAC
    AACCCCTTCA TCTGCTCCTC CAGTAAGGAC AACGGCATGA AGCGCTGCAG TGCTGTGCCA
    CCCCTTAAAG AGGGTGTTGA GTGCACTTTA AATGCATCTT TTCTGGGACA TGTCTACTCT
    GGCCTGAGCG GGACAGGCAA CTACAGCTGT GTGAACTGGA ACCAGTATTA CAGTGAATGC
    AAGCCCGGAG ACTTAAACCC GCACAAAGGC GCAGTAAACT TTGATAACAT TGGATATGCC
    TGGATCGCTA TATTCCAGGT TATTACATTG GAAGGATGGG TGGACATCAT GTATTACGTA
    ATGGATGCCC ACTCCTTTTA CAATTTTATA TATTTTATAT TGCTTATTAT AGTGGGTTCC
    TTCTTCATGA TCAACCTGTG CCTGGTTGTA ATAGCAACCC AGTTCGCAGA AACCAAACAG
    CGCGAGAACA TGCTGATGAA GGAGCAGCGT GCACGATACC GCTCCAACGA CAGCACCCTC
    GCCAGCTACT CTGAGCCGGG CAGCTGCTAC GAAGAGATGC TCAAGTACGT CAGCCACCTG
    TACCGCAAGG TGAAGCGCCG CCTCTCCCGC ATCTACAACA GCTGGCAGAG CAAGCGGCGA
    AAGAAAGTGA ACCCCAACAC CGGGGCTGGC GGAGGTGCCA ATGGTCACAG TAGGCACAGG
    GGTTACGGAC ACTGGGTACA GTCCATTCAC AGCCTCATCC AGCAGCACCA GCGACAGCAC
    TGTCACCTCA GCAATGGCAG CCCCAGTCCT GTGGCCACTA CGGGCTCCAG TGAGGCTCTG
    GAAATGAGGT CCATCACAGC GGGTCAGCAG CTGACCGTGT CCTCTCAGCC AACCGCCACG
    CACAATCTTT CTTCCAGCAC ACAATCAGTG CATAGCATTT ATCAAGGTGA CTTTCGTGAG
    GCCCCGCAGG AGCGAACACC CACTAATGTT GCTGTTCCGG CACTCAGCCG ACTCAATGGG
    GGGATGAACT ATCCCACAAT TTTGCCGTCC CTCATTTGCA ACTATTCAGG TGGAGGTTCA
    TTAGGGAAAG AGAGGGGGAA GTCGGATGCA AATACAGGAC ATGAGGTGAT ACAGGACTTT
    GACAAGCTGC ACCAGCAAAT TGAAGCACAT AGCCACAGTC GTGTGCTGCT GCAGATGGCT
    GGTGTGGTGC CCACTCAATT ACTTCTTGAG GTCATGAGCT GCCCGTATTG CGTAAGGGCC
    CTTGAGAGCA TGGATCTGGA GATTGGTGAT TCAGACTACC CTCGATCAGA AGGGGACATG
    GTGTATGAGT TCTCATATGA TGCCGGGTTC ATAACCGGCC GTTCAGCACA TCATAAAATG
    CAGGAGAAAC AAAACCGTCT GGTTCAATTC TGGGAGGACT TCAGGGAAAG ACTGACCCGT
    ATAGTGGATA GCAAATACTT TAACCGGGGA ATTATGATCG CCATCCTCAT CAACACCTTG
    AGTATGGGGA TTGAATACCA TGAGCAGCCC GAGGAGCTGA CCAACATCCT GGAGATCAGC
    AACATCGTCT TCACTAGCAT GTTTGTCCTC GAAATGCTCT TCAAACTGCT GGCCTTTGGC
    ATCTTCGGCT ATATAAGGAA CCCATATAAC ATCTTTGATG GGGTTATTGT TGTCATAAGT
    GTATGGGAGA TCATCGGTCA TGCGGATGGT GGTCTCTCAG TGCTGAGGAC TTTTCGTCTC
    CTGAGGGTGC TCAAACTGGT GCGCTTCCTC CCTGCCCTGC GCAGACAGCT GCTTGTCCTG
    ATGAAGACCA TGGACAATGT GGCAACATTT TGCATGCTAC TCATGCTCTT CATCTTCACT
    TTCAGCATAC TCGGAATGCA TCTGTTCGGA TGCAAATTCA GCCTGAAGAT GGAGAATGGA
    GACACTATTC CGGACAGGAA AAACTTTGAT TCCCTGCTGT GGGCCATTGT CACTGTGTTT
    CAGATTCTGA CCCAGGAAGA CTGGAACGTG GTCCTCTATA ATGGAATGGC TTCCACCAGC
    CCATGGGCGG CTCTTTATTT TGTTGCCCTC ATGACGTTTG GGAATTATGT GCTCTTTAAT
    CTACTGGTGG CCATTTTAGT GGAAGGGTTT CAGGCGGAGG GAGATGCTAA CAAGTCAGAC
    GGAGATGAGG AAAAGACATC AGTGAACTCT GAAGAGAAAA TGGAGCAGCT GCAAAGTTCA
    GCCAATGGGC ATGTGGATCC TTGTGGCACT TTGCCTCCAC CAATCATCAT GCGCACTGCT
    GCCACACCCA TGCCCACTCC AAAGAGCTCT CTGGGGCCCG AGTCTGTGTT TGACTTGACC
    GAGTCCAGAC GGGGCAGTGC TGTCTCCATA GATCCAAATG CTTTTGATCA GAAGTCCCTG
    TCCAGTCCAA GAAGATCACC ATGCCACCCC CGTGGCTCGG GCAGTAACTG GGGCAGCCGC
    AGGTCGTCCT GGAACAGCTT AGGACGAGCG CCAAGTCTGA AAAGGAAGGA CACATCTGGG
    GAGCGCGAGT CTCTGCTGTC CGGGGAAGGC AGGGGCAGCT TTGAGGATGA CGACATAGAG
    GCGGAGAAGC TGACTAACAT CAGCGGAGGC TCCCTGCAGC ACCCCAGCTC TCTGGACATC
    CCAGAACTGC CTCAAATGGG GGAATACCTC GACTGCAATG GACGAAGCCA ACACATACTC
    GCTGACCTGT CTGCCAGTCT CAACAAGGAG GACTCCATCG CTGAGGAGGA CTTGGATGAT
    AGCTTCTGTT TCCGTCTCAG GAGGACCCTG GCAGCTTATA AACCCAAGTG GTGCAAAGAT
    CATGAGGAAT GGTCACTTTA CCTGTTCTCT CCTCATAACA AGTTTCGGAT GATGTGTCAA
    AAACTCATCT CTCACAAGAT GTTTGATTAT GTAGTTTTAG TATTCATCTT CCTAAACTGC
    ATCACTATTG CTCTGGAGAG ACCCCATATT CAACAATCTG AGCGATTATT TCTCCTCGTC
    TCAAACTATG TTTTCACTGT GATATTTGTT GCTGAAATGA CAGTGAAGGT TGTGGCCCTT
    GGTTTCTACT CCGGGAACCA GAGTTATCTA AAGAGCACAT GGAATGTTTT GGATGGGGTG
    CTGGTCTTTG TGTCCCTCAT TGACATTCTG GTCTCTCTGG CCTGGACTGG AAACCGAATC
    TTTGGCATCC TGAGGGTTCT TCGCCTGCTG CGAACTCTGC GGCCACTGAG AGTAATTAGT
    CGTGCTCCTG GTCTGAAGCT GGTAGTAGAA ACGTTGATAA CGTCCCTGAG GCCCATTGGG
    AACATTGTGC TGATCTGCTG TGCTTTCTTT ATTGTTTTCG GCATTCTTGG AGTTCAGCTC
    TTCAAAGGAA AGTTTTTCCA CTGTGAAGGC GGCGATACCA GAAACATCAC CAATAAGTCT
    GACTGTCTTC AGGCAAACCT CAAATGGATC AGAAGAAAGT ACAATTTTGA CAACTTGGGA
    CAGGCTCTCA TGTCTCTTTT CGTGCTTTCC TGTAAGGATG GATGGGTCAA TATAATGTAT
    GATGGGTTGG ATGCTGTTGG TGTGGACCAG CAGCCTGAGC GCAACCACAA CCCCTGGATG
    CTGTTATATT TTATTTCCTT TCTGCTAATC GTCAGCTTCT TCGTCCTGAA TATGTTTGTC
    GGAGTAGTGG TGGAAAACTT CCACAAGTGC CGTCAAGACC AGGAGGAAGT GGAGGCGCGT
    CTGCTGGAGT TGAAGAGGCA AAAACTGATG GAGAAAAAGC GCAGGAGTAA GGAGAATTGC
    GGGTCTGAGG CACTCCGGAG ACCATATTAT GCTGACTATT CTCCAGCCCG GCTTTACATC
    CACACACTGT GCACCAACCA CTATTTGGAC CTGTTTATAA CCGGCATCAT CTGCATCAAC
    GTAGTTACCA TGTCCATCGA GCATTTCAAT CAGCCTTCTT ATCTTGACGA GGCACTTAAA
    TACTGTAACT ATGTATTTAC AATCATCTTC ATAATTGAGG CTCTGCTTAA ACTCGTGGCA
    TTTGGAATAC GGAGATTCTT CAAAGACAGA TGGAACCAGC TGGATCTTGC AATAGTCCTA
    CTCTCTATCA TGGGCATCAC TCTGGAGGAG ATTAAGATGA ATGCAGCGCT GCCCATCAAC
    CCCACCATCA TCCGTATTAT GAGAGTGCTA AGAATCGCAA GAGTGCTGAA GCTGTTAAAG
    ATGGCTACAG GCATGAGATC CCTACTCGAC ACCGTAATGC AAGCTTTACC ACAGGTGGGA
    AATCTGGGCT TGCTGTTTAT GCTTCTCTTC TTCATTTATG CGGCGCTTGG CGTGGAGCTT
    TTTGGGAAAC TAGAATGCAC AGAGAACAAT CCGTGTGAAG GATTAAGTCC ACATGCCACG
    TTTGAGAACT TTGGAATGGC ATTTTTGACA CTTTTCCGAG TATCAACTGG GGACAATTGG
    AATGGAATAA TGAAGGACAC TCTGCGAGAG TGCCTTCCTT CAGAGACGCA ATGCCTCAGC
    TATCTACCCT GGGTCTCTCC AATCTATTTT GTAACCTTTG TTCTAATGGC TCAGTTTGTG
    CTGGTTAACG TGGTCGTAGC TGTGTTGATG AAACATCTGG AGGAGAGCAA TAAAGAAGCT
    AAAGAGGATG CAGAAATGGA TGCTGCGATC AAGTTGGCAA TTTTGAAAGA AACTCGCCGT
    CTCAGCACCA TCTCAGCCAG TGCCACTGCG GGACCAGAAG GCAGGGCAAC TTATATCGAG
    CCACACCCAG ATTCACCTGA GCAGGATGAG GGGATGCAAA GAACACAGGG GAATTTGCTC
    TCTCCGCGGA AAATGTCAGT GTCAAGGATG CACTCTTTAC CCAATGACAG CTACATGTTT
    CGCCCTGTGA GGCCAGCCAG TGCTCCGTAT CCTCTAGAAG AAGTGGAGAC CTGTCAAGAT
    TATCTTGAAT TAGGTTCCAT CACCTCGGTT CACTCCCAGC CATGCGAAAG CCTTTCCCTG
    CTCCAGGTCC CTGGTGTTCC TCCCCATTCC CAGTTCAACT GCCTCCCCAA GATCCCTCCT
    CCCATGCCCT CTCCAACCCA CAGCATAGAC AGGATTTTAC GCAGACAGAG GGCAATTAAA
    AATGACTCCA TTGACATGCA TAATTTCGAC TCCAAAGAAA ATGTGGAAAG ACAGAAAGGG
    ATAACCACAG GCAGCTTCAA GCTGCATGTT CCTCAAATCA CCGGCATCTC TCAGGGGTCT
    CCCTTGCTGC CACCACGTGG ACCTAGTGTC CACACCTTCC AGCAGCCGCA CAGCCAGCAC
    AATATCTCTT CTCGACCTCC CAGTCTGGCC AGCAGCAGCA GAAGCACCAG CCCCAGTGGC
    TCAATGTTTG ACTCCGCTGA CCCAACCGAT GAGGAGGTGC GCCACATCAA TAGCACAGCC
    CGCCTGTGGA TCGGGGTTCA TGCAGAAGCA AGGAGTCACT CCCTCTCTCC TTACAGCACC
    TCAGGGACGC TGCTTCCCAT CCCCGTGAAA AACTGCAGCA GTGCTGCCAA CTTAACCATC
    AAAGACCCCA AGAAGTGCTT CAGTGTGGAC ACTGAGGGTT TTCTGGAAAG ACCCTACGCT
    TCTGATGACC ATCGCAGGCA CTCGATTGAG ATTTGCTCCT CAGGAGACGA TGGGCTGTTT
    GGTCAAGAAC TCTGTGAAAA GAGGCACGTT CCCACGCGTG GAAAGTCTTT GCACATTCCC
    CGGAAGACAA AGATGAGCCC TCCCTGCATC TCCATCGAGC CCCCATTTGA AAAGGATGTT
    TCCCCCTCTC ATGCGTCCAT GAAAAAGTTG TCGGATAGCA TGTTACTGCG GAGGAGGACA
    CCATCCTATG ACCTGGCCCT GCAGCGGGAC TCTCTGGACT TGCCGGAGAA CCAGCTGTCA
    ACCCAGCCAC TCATCAAAGT AGAGCGACAT CCCTCTCACT GTGCCGAGTA TCTGTCCCTC
    CCACACCCCG CCCCAATGAG CGGTCTGGCA GGCGTTTTGT GTGAAAGCAC GCAGCCGCCC
    CCCTTTGATA GCAGATATGA AGAATCCACG GACTTCGGCT CTGTAAACAG GACAGCTCAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.