CACNA1H cDNA ORF clone, Bos taurus(cattle)

The following CACNA1H gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1H cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa230681 XM_024985428.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 H (CACNA1H), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa230682 XM_024985429.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 H (CACNA1H), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa230683 XM_024985430.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 H (CACNA1H), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa230684 XM_024985431.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 H (CACNA1H), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa88470 XM_015460299.1
Latest version!
Bos taurus calcium channel, voltage-dependent, T type, alpha 1H subunit (CACNA1H), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa95201 XM_015469221.1
Latest version!
Bos taurus calcium channel, voltage-dependent, T type, alpha 1H subunit (CACNA1H), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa230681
    Clone ID Related Accession (Same CDS sequence) XM_024985428.1
    Accession Version XM_024985428.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7056bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1H isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGACCGAGG GCGTGCAGGC GGCCGACGAG GTCCGCGTGC CCCTGGGCGC GCCAGCCCCG 
    GGCCCGGCGG CGGCGGCGGC GTCCCCGGCG AGCCCTGGGG CCCCGGGGCC AGAGGCGGAG
    CGGGGGTCGG GGCCCAGTGC GTCGCCCCCC GAGAGTCCGG CGGCCGAGCG CGGCGCCGAG
    CTGGGCGCCG ACGAGGAGCA GCCCGTCCCG TACCCGGCGC TGGCGGCCAC CGTCTTCTTC
    TGTCTCGGGC AGACCACGCG GCCGCGCAGC TGGTGTCTCC GGCTGGTCTG TAACCCGTGG
    TTCGAGCATG TCAGCATGCT GGTCATCATG CTCAACTGCG TGACCCTGGG CATGTTCCGG
    CCCTGCGAGG ACGTCGAGTG CCGCTCGGAG CGCTGTGGCA TCCTCGAGGC CTTTGACGAC
    TTCATCTTCG CCTTCTTCGC GGTGGAGATG GTCATCAAGA TGATCGCCCT GGGGCTGTTT
    GGGCAGAAGT GCTACTTGGG TGACACGTGG AACAGGCTGG ACTTCTTCAT CGTCATGGCG
    GGTATGATGG AGTACTCCTT GGACGGACAC AACGTGAGCC TCTCGGCCAT CCGAACCGTG
    CGCGTGCTGC GGCCCCTTCG TGCCATCAAC CGTGTGCCCA GCATGCGGAT CCTGGTCACG
    CTGCTGCTGG ACACACTGCC CATGCTCGGC AACGTTCTCC TGCTCTGCTT CTTTGTCTTC
    TTCATCTTCG GCATCGTGGG CGTCCAGCTT TGGGCCGGCC TGCTGCGCAA CCGCTGCTTC
    CTGGACAGCA CCTTCGCCAG GAACAGCAAC CTCAGCTTCC TGCGGCCGTA CTACCAGCCG
    GAGGAGGGCG AAGAAAACCC GTTCATCTGC TCCTCCCGCC GGGACAACGG CATGCAGAAG
    TGCTCGCACA TCCCCAGCCG CCGCGAGCTG CGCGTGGAGT GCACGCTGGG CTGGGAGGCC
    TACGGGCAGC CGCAGGCCGA GGGCGCAGGT GGCACGGGTC ACCACACCTG CATCAACTGG
    AACCAGTACT ACAATGTGTG CCGCTCGGGC GACTCCAACC CGCACAACGG GGCCATCAGC
    TTCGACAACA TCGGCTACGC CTGGATCGCC ATCTTCCAGG TGATCACGCT GGAGGGCTGG
    GTGGACATCA TGTACTACGT CATGGACGCC CACTCTTTCT ACAACTTCAT CTACTTCATC
    TTGCTTATCA TCGTGGGCTC CTTCTTCATG ATCAACCTGT GCCTGGTGGT CATCGCCACG
    CAGTTCTCAG AGACGAAGCA GCGGGAGAAC CAGCTGATGC GGGAGCAGCG GGCCCGTTAT
    CTGTCCAATG ACAGCACGCT AGCCAGCTTC TCTGAGCCGG GCAGCTGCTA TGAGGAGCTT
    CTCCGGTACG TGGGCCACGT GTGCCGCAAG CTAAAGCGCC GTGGCCTGCG CCTCTACGCC
    CGCTGGCAGA GCCGCTGGCG CAAGAAGGTG GAGCCCGGTG GCGCCACGCA TGGCCAGGGC
    TCAGGGCGGC GGCCGCGGCG GGCAGGCGGG CGCGCTGCCT CCATCCACCA CCTCGTGTAC
    CACCACCACC ACCATCACCA CCACCACTAC CACTTCAGCC ACGGCAGCCC CCGCCGGCCA
    GGCCCCGAGC CAGGAGCCGG CGACACTAGG CTGGTGCGGG CCGGAGCGCC AGCCTCACCC
    GGGCGCGGGC CCCCTGACGC CGAGTCGGTG CACAGTGTGT ACCACGCTGA CTGCCACGTG
    GAGGGGCCAC AGGAGAGGGC GCGCGTGGCA CACGCCGCAG CCACTGCCGC TGCTGGGCTC
    AAGCTGGCCA CCGGGCTGGG AGCCATGAAC TACCCCACCA TCCTGCCCTC GGCTGCGGGC
    AGTGGCAAAA GCGGCCTCGG GCCCAAGGGG AAGCGGCCTG GTGGCCCCCC GGGAGCCGGG
    GGGCACAGCC CCCTGAGGCT GAGCAGCCCG GACCCCTGCG AGAAGATCCA GCACCTGGTT
    GGGGAGCATG GACTGGGCCA GGCCCCCAGC CGCCTGTCAG GCCTGAGTGT GCCCTGCCCC
    CTGCCCAGCC CCCAGGCGGG CACGCTGACC TGTGAGCTGA GCAGCTGCCC GTACTGCACC
    AGTGCCCTGG AGGGCCCCGA GCTGGAGTTC AGCGACTCAG ACAGTGGAGA CTCAGACAGC
    AATGGGGTCT ACGAGTTCAC GCAGGATGTG CGGCACGGGG ACCACCGCGA CCCCATGCAG
    TCGCCCCCCG TGGCAGAGGC GCCAGGCGTG GGCGGCACAC GGCGGAGGGG GCCGCAGCGG
    GCGGTGGCAG GCGAGCAGGG TGGGCTGGGC CACGTCTGGG CCTCCTTCAG TGGCAAGCTG
    CGCCGCATCG TGGACAGCAA GTACTTCAAC CGGGGCATCA TGGTGGCCAT CCTCACTAAC
    ACGCTGAGCA TGGGTGTCGA GTACCACGAG CAGCCCGATG AGCTGACCAA CGCCCTGGAG
    ATCAGCAACA TTGTGTTCAC AAGCATGTTT GCTCTGGAGA TGCTGTTGAA GCTGCTGGCC
    TGCGGCCCAC TGGGCTACAT CCGGAACCCT TACAACATCT TTGACGGCAT CATCGTAGTC
    ATCAGCGTGT GGGAGATCAT CGGGCAGGCG GACGGCGGGC TGTCGGTGCT GCGCACCTTC
    CGGCTGCTGC GGGTGCTCAA GCTGGTGCGC TTCATGCCCG CACTGCGGCG CCAGCTGGTG
    GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTTTTCATC
    TTCATCTTCA GCATCCTTGG CATGCATCTC TTTGGCTGCA AATTCAGTCT GAAAACAGAT
    ACCGGAGACA CAATCCCCGA CCGGAAGAAC TTTGATTCCC TACTGTGGGC CATCGTCACC
    GTGTTCCAGA TCCTCACCCA GGAGGACTGG AACGTCGTCC TGTACAACGG CATGGCCTCC
    ACGTCCTCCT GGGCCGCCCT GTACTTCGTG GCCCTCATGA CCTTTGGCAA CTACGTGCTC
    TTCAACCTGC TGGTGGCCAT CCTGGTGGAG GGTTTCCAGG CCGAGGGTGA TGCCACCAGG
    TCCGACACAG ATGAAGACAA GACATCCACC CACTTGGAGG AGGACTTGGA CAAGTTCCGA
    GACCTCAGGG CCACAGAGAT GAAGATGTAC TCGCTGGCAG TGACACCCAA CGGGCACCTG
    GAGGGCCGGG GCAGCCTGCC CCCTCCCCTT ATCATGCGCA CAGCGGCCAC GCCCATGCCC
    ACCCCCAAGA GCTCCCCACA CCTGGACGAG GCCCCTGGCC CCCTGGACTC GCGCCGTGGC
    AGCAGCAGCT CCATGGACCC TCAGCTAGGA GACCAGAAAT CTCTGTCCAG CCTCCGCAGC
    TCGCCCTGCG CCCCCTGGGG GCCCAACAGC GCCTGGAGCA GCCGGCGCTC CAGCTGGAAC
    AGCCTGGGCC GTGCACCCAG CCTCAAGCGC AGGAGCCAGT GTGGGGAGCG CGAGTCGCTG
    CTGTCCGGTG AGGGCAGGGG CAGCGGCAGC ACTGACGAGG AGGCCGAGGA TGGCCGGCCC
    GGGGCGGGGG CCTCGCCGGG TACGCGTGCC ACGCCGCTGC GGCGCACCGA GTCCCTGGAC
    CACCGTAGCA CGCTGGACCT GCGGCCCCCA CGGCCAGCCA CCCTGCTGCC CACCAAGGTC
    CACGACTGCA ACGGGCAGGT GCTGGCCCTG CCGAGCGAGT TCTTCCTGCG CATCGACAGC
    CACAAGGAGG ATCCGGCCGA GTTTGACGAC GATGTGGAGG ACAGCTGCTG CTCAAGGCTG
    CAGAAGGTGC TGGAGCCGCA TAAGCCGGAG TGCTGCCGGA GCCGCGAGCC CTGGGCCCTG
    TACCTCTTCT CCCCACAGAA CAGGTTCCGC ATCTCCTGCC AAAAGATCAT TGCTCACAAG
    ATGTTCGATC ATGTCGTCCT CGTCTTCATC TTCCTCAACT GCATCACCAT CGCCCTGGAG
    AGGCCCGACA TCGACCCCGG CAGCACTGAG CGCGTCTTCC TCAGCGTCTC CAACTACATC
    TTCACAGCGA TCTTCGTGGC GGAGATGATG GTGAAGGTGG TGGCCCTGGG TCTCATCTCG
    GGTGAGCATG CCTACCTGCA GAGCAGCTGG AACATACTGG ATGGGGTGCT GGTCCTGGTG
    TCCCTGGTGG ACATCATCGT GGCCATGGCC TCGGCTGGCG GCGCCAAGAT CCTGGGCATC
    CTGCGTGTGC TGCGCCTGCT GCGGACCCTG CGGCCGCTAA GGGTCATCAG CCGTGCGCCG
    GGACTCAAGC TGGTGGTGGA GACTCTGATA TCGTCCCTCA GGCCCATCGG GAACATCGTC
    CTCATCTGCT GCGCCTTCTT CATCATCTTC GGCATCCTCG GGGTGCAGCT CTTCAAGGGG
    AAGTTTTACT ACTGCGAGGG CGCCGACACC AGGAACATTT CCACTAAGGC CGAGTGCAGG
    GCCGCGCACT ACCGCTGGGT GCGACGCAAG TACAACTTCG ACAACCTGGG CCAGGCGCTG
    ATGTCGCTGT TCGTGCTCTC GTCCAAGGAC GGCTGGGTGA ATATCATGTA CGACGGGCTG
    GACGCCGTGG GCGTGGACCA GCAGCCCGTG CCCAACCACA ACCCCTGGAT GCTGCTCTAC
    TTCATCTCCT TCCTGCTCAT CGTCAGCTTC TTCGTGCTCA ACATGTTCGT GGGCGTCGTG
    GTGGAGAACT TCCACAAGTG CCGGCAGCAC CAGGAGGCCG AGGAGGCGCG GCGGCGCGAG
    GAAAAGCGGC AGCGTCGCCT GGAGAGGAAG CGCAGGAGCA CTTTCCCTAA CCCAGAGGCC
    CAGCGACGGC CCTACTATGC GGACTACTCG CCCACCCGCC GCTCCATCCA CACTCTGTGC
    ACCAGCCACT ACCTGGACCT CTTCATCACC TTCATCATTG GCGTCAACGT CATCACCATG
    TCCATGGAGC ACTACAACCA GCCCAAGGCT CTGGACGAGG CCCTCAAGTA CTGCAATTAC
    GTGTTCACCA TCGTCTTTGT CGTCGAGGCC GTGCTGAAGC TGGTGGCCTT TGGGTTCCGG
    AGGTTCTTCA AGGACAGGTG GAACCAGCTG GACCTGGCCA TCGTTCTGCT GTCCATCATG
    GGCATCACGT TGGAGGAGAT CGAGATGAAT GCAGCGCTGC CCATCAACCC CACCATCATC
    CGAATCATGC GTGTCCTCCG TATCGCCCGC GTCCTGAAGC TGCTCAAGAT GGCCACGGGC
    ATGCGGGCCC TGCTGGACAC GGTGGTCCAG GCGCTGCCCC AGGTAGGGAA CCTCGGCCTG
    CTTTTCATGC TCCTGTTTTT TATCTATGCT GCCCTGGGAG TGGAGCTGTT CGGGAGGCTA
    GAATGCAGTG AGGACAACCC CTGCGAGGGC CTGAGCAGAC ACGCCACCTT CTCTAACTTC
    GGCATGGCCT TCCTCACGCT GTTCCGCGTG TCCACCGGGG ACAACTGGAA CGGGATCATG
    AAGGACACGC TGCGCGAGTG CGCCCGCGAG GACAAGCACT GCCTCACCTA CCTGCCGGCC
    ATCTCGCCCA TCTACTTCGT CACCTTCGTG CTGGTGGCCC AGTTCGTGCT TGTCAACGTG
    GTGGTGGCCG TGCTCATGAA GCACCTGGAG GAGAGCAACA AGGAGGCCCG CGAGGACGCC
    GAGCTGGACG CAGAGCTTGA GATGGAAATG GCGCAGGGGT CCCCCGCCCG TCCCAGGCCG
    GCGGCCCCTC AGAGCCCCGG CGCCTCGCCG GACTCCCCCA ACGTGCTGGT CGTGCGCAAG
    GTGTCGGTGT CCAGGATGCT ATCCCTGCCC AACGACAGCT ACATGTTCCG GCCCGTGGCG
    CCTGCGGCAG CCGCTCACCC CCACCCGCTA CAGGAGGTGG AGATGGAGAC CTACGCGGGC
    GCGGCCGCCG GCCAGGTCAC CGCTGCCCAC TCGCCGCCTG TGGAGTCCTG CGCCTCCCTC
    CAGGTTCCGT CCGCCGTGTC CTCCCCGTCC AGGGGCGGCG ACACCCTCCG TGCCCTGCCC
    CCACGGGGTG CAGCCCGGTC GCCTAATGTC GGCCGACTTC TCTGCAGACA GGAGGCCGTC
    CACACGGAGT CCCTGGAAGG GCATGTCGAT GGCACTGAGG ACAGCAGCCC ATTCTGGGGA
    GAGCCTGGCG GGAAGACCCC CGTGAGACAG GCATCCCTGG GGCCCTCCTT GCGGTCCCCA
    TCCCGGTCCC CGCGACCCTC CAGCATCCGC ACCCGCAAGC ACACTTCTGG ACAGCACTGC
    ATCTCCAGCC GGCCTCCTGC CCCAGGCGGG GAGGAGTCCG AAGCCCCAGA CCCAGCTGAC
    GAGGAGGTCA GCCACATCAC CAGCTCTGCC CGCAGCCCTT TGGCCTCGCC CACTGCCTGT
    GGGATCGTGG GCGGCGAGCC AGACGTGCAC AGGCTCTACA GCGTGGACGC CCGGGGCTTC
    CTGGACCAGC CGGGCCGGGT GGATGAGCAG AGGCGGCCCT TGGGGGAGCC AGGAGTTGGG
    GACAGCCGTC CAGAGGCCGG GGAGGCGAAG CCCCGGGTCC TGGAGGCCGA GCTGGCCCTA
    GGGGCGCGCA GGAAGAAGAA GATGAGCCCC CCCTGCATTT CCATAGAGCC CCCCGCTGAG
    GACGAGGGTG CGGCCAGGGC CCCTGCGGCA GAAGGCGGCA GCACCACCCT GCGGCGGCGA
    ACCCCATCCT GCGAGGCTGT GCCCCATAGG GACCCCCTGG AGCCCGCAGA CAGCACAGGG
    CTGGACGCTG CTGCCAAGGG GGAGCGGCGG GGCCAGGGCC CCTGCCGTAC AGAACACCTG
    ACTGTCCCGA ACTTCACCTT TGAGCCACTG GACGTGGGGA GCCCCAGTGG GGACCTGTTC
    ACAGATGCGG GCCATGGGGC CACCCCAGAA CCCAGACCCT CCTCCTCAGG CACCGCGGCC
    CCTCCAGAAC CCCGGGAGAT GGAGTCCACA GTGCCCTCAG GAGACCCCCT AGAGGAGGGG
    CGGGGGGTGC ACCTCACTGT CCCGGAGAGC CCACCGAAGA GAGCGTCCCC CCCGGCTGTG
    CCAGTCCCGG GTGACGACGT GGATGAGCCC GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841196.1
    CDS97..7152
    Translation

    Target ORF information:

    RefSeq Version XM_024985428.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 H (CACNA1H), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024985428.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGACCGAGG GCGTGCAGGC GGCCGACGAG GTCCGCGTGC CCCTGGGCGC GCCAGCCCCG 
    GGCCCGGCGG CGGCGGCGGC GTCCCCGGCG AGCCCTGGGG CCCCGGGGCC AGAGGCGGAG
    CGGGGGTCGG GGCCCAGTGC GTCGCCCCCC GAGAGTCCGG CGGCCGAGCG CGGCGCCGAG
    CTGGGCGCCG ACGAGGAGCA GCCCGTCCCG TACCCGGCGC TGGCGGCCAC CGTCTTCTTC
    TGTCTCGGGC AGACCACGCG GCCGCGCAGC TGGTGTCTCC GGCTGGTCTG TAACCCGTGG
    TTCGAGCATG TCAGCATGCT GGTCATCATG CTCAACTGCG TGACCCTGGG CATGTTCCGG
    CCCTGCGAGG ACGTCGAGTG CCGCTCGGAG CGCTGTGGCA TCCTCGAGGC CTTTGACGAC
    TTCATCTTCG CCTTCTTCGC GGTGGAGATG GTCATCAAGA TGATCGCCCT GGGGCTGTTT
    GGGCAGAAGT GCTACTTGGG TGACACGTGG AACAGGCTGG ACTTCTTCAT CGTCATGGCG
    GGTATGATGG AGTACTCCTT GGACGGACAC AACGTGAGCC TCTCGGCCAT CCGAACCGTG
    CGCGTGCTGC GGCCCCTTCG TGCCATCAAC CGTGTGCCCA GCATGCGGAT CCTGGTCACG
    CTGCTGCTGG ACACACTGCC CATGCTCGGC AACGTTCTCC TGCTCTGCTT CTTTGTCTTC
    TTCATCTTCG GCATCGTGGG CGTCCAGCTT TGGGCCGGCC TGCTGCGCAA CCGCTGCTTC
    CTGGACAGCA CCTTCGCCAG GAACAGCAAC CTCAGCTTCC TGCGGCCGTA CTACCAGCCG
    GAGGAGGGCG AAGAAAACCC GTTCATCTGC TCCTCCCGCC GGGACAACGG CATGCAGAAG
    TGCTCGCACA TCCCCAGCCG CCGCGAGCTG CGCGTGGAGT GCACGCTGGG CTGGGAGGCC
    TACGGGCAGC CGCAGGCCGA GGGCGCAGGT GGCACGGGTC ACCACACCTG CATCAACTGG
    AACCAGTACT ACAATGTGTG CCGCTCGGGC GACTCCAACC CGCACAACGG GGCCATCAGC
    TTCGACAACA TCGGCTACGC CTGGATCGCC ATCTTCCAGG TGATCACGCT GGAGGGCTGG
    GTGGACATCA TGTACTACGT CATGGACGCC CACTCTTTCT ACAACTTCAT CTACTTCATC
    TTGCTTATCA TCGTGGGCTC CTTCTTCATG ATCAACCTGT GCCTGGTGGT CATCGCCACG
    CAGTTCTCAG AGACGAAGCA GCGGGAGAAC CAGCTGATGC GGGAGCAGCG GGCCCGTTAT
    CTGTCCAATG ACAGCACGCT AGCCAGCTTC TCTGAGCCGG GCAGCTGCTA TGAGGAGCTT
    CTCCGGTACG TGGGCCACGT GTGCCGCAAG CTAAAGCGCC GTGGCCTGCG CCTCTACGCC
    CGCTGGCAGA GCCGCTGGCG CAAGAAGGTG GAGCCCGGTG GCGCCACGCA TGGCCAGGGC
    TCAGGGCGGC GGCCGCGGCG GGCAGGCGGG CGCGCTGCCT CCATCCACCA CCTCGTGTAC
    CACCACCACC ACCATCACCA CCACCACTAC CACTTCAGCC ACGGCAGCCC CCGCCGGCCA
    GGCCCCGAGC CAGGAGCCGG CGACACTAGG CTGGTGCGGG CCGGAGCGCC AGCCTCACCC
    GGGCGCGGGC CCCCTGACGC CGAGTCGGTG CACAGTGTGT ACCACGCTGA CTGCCACGTG
    GAGGGGCCAC AGGAGAGGGC GCGCGTGGCA CACGCCGCAG CCACTGCCGC TGCTGGGCTC
    AAGCTGGCCA CCGGGCTGGG AGCCATGAAC TACCCCACCA TCCTGCCCTC GGCTGCGGGC
    AGTGGCAAAA GCGGCCTCGG GCCCAAGGGG AAGCGGCCTG GTGGCCCCCC GGGAGCCGGG
    GGGCACAGCC CCCTGAGGCT GAGCAGCCCG GACCCCTGCG AGAAGATCCA GCACCTGGTT
    GGGGAGCATG GACTGGGCCA GGCCCCCAGC CGCCTGTCAG GCCTGAGTGT GCCCTGCCCC
    CTGCCCAGCC CCCAGGCGGG CACGCTGACC TGTGAGCTGA GCAGCTGCCC GTACTGCACC
    AGTGCCCTGG AGGGCCCCGA GCTGGAGTTC AGCGACTCAG ACAGTGGAGA CTCAGACAGC
    AATGGGGTCT ACGAGTTCAC GCAGGATGTG CGGCACGGGG ACCACCGCGA CCCCATGCAG
    TCGCCCCCCG TGGCAGAGGC GCCAGGCGTG GGCGGCACAC GGCGGAGGGG GCCGCAGCGG
    GCGGTGGCAG GCGAGCAGGG TGGGCTGGGC CACGTCTGGG CCTCCTTCAG TGGCAAGCTG
    CGCCGCATCG TGGACAGCAA GTACTTCAAC CGGGGCATCA TGGTGGCCAT CCTCACTAAC
    ACGCTGAGCA TGGGTGTCGA GTACCACGAG CAGCCCGATG AGCTGACCAA CGCCCTGGAG
    ATCAGCAACA TTGTGTTCAC AAGCATGTTT GCTCTGGAGA TGCTGTTGAA GCTGCTGGCC
    TGCGGCCCAC TGGGCTACAT CCGGAACCCT TACAACATCT TTGACGGCAT CATCGTAGTC
    ATCAGCGTGT GGGAGATCAT CGGGCAGGCG GACGGCGGGC TGTCGGTGCT GCGCACCTTC
    CGGCTGCTGC GGGTGCTCAA GCTGGTGCGC TTCATGCCCG CACTGCGGCG CCAGCTGGTG
    GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTTTTCATC
    TTCATCTTCA GCATCCTTGG CATGCATCTC TTTGGCTGCA AATTCAGTCT GAAAACAGAT
    ACCGGAGACA CAATCCCCGA CCGGAAGAAC TTTGATTCCC TACTGTGGGC CATCGTCACC
    GTGTTCCAGA TCCTCACCCA GGAGGACTGG AACGTCGTCC TGTACAACGG CATGGCCTCC
    ACGTCCTCCT GGGCCGCCCT GTACTTCGTG GCCCTCATGA CCTTTGGCAA CTACGTGCTC
    TTCAACCTGC TGGTGGCCAT CCTGGTGGAG GGTTTCCAGG CCGAGGGTGA TGCCACCAGG
    TCCGACACAG ATGAAGACAA GACATCCACC CACTTGGAGG AGGACTTGGA CAAGTTCCGA
    GACCTCAGGG CCACAGAGAT GAAGATGTAC TCGCTGGCAG TGACACCCAA CGGGCACCTG
    GAGGGCCGGG GCAGCCTGCC CCCTCCCCTT ATCATGCGCA CAGCGGCCAC GCCCATGCCC
    ACCCCCAAGA GCTCCCCACA CCTGGACGAG GCCCCTGGCC CCCTGGACTC GCGCCGTGGC
    AGCAGCAGCT CCATGGACCC TCAGCTAGGA GACCAGAAAT CTCTGTCCAG CCTCCGCAGC
    TCGCCCTGCG CCCCCTGGGG GCCCAACAGC GCCTGGAGCA GCCGGCGCTC CAGCTGGAAC
    AGCCTGGGCC GTGCACCCAG CCTCAAGCGC AGGAGCCAGT GTGGGGAGCG CGAGTCGCTG
    CTGTCCGGTG AGGGCAGGGG CAGCGGCAGC ACTGACGAGG AGGCCGAGGA TGGCCGGCCC
    GGGGCGGGGG CCTCGCCGGG TACGCGTGCC ACGCCGCTGC GGCGCACCGA GTCCCTGGAC
    CACCGTAGCA CGCTGGACCT GCGGCCCCCA CGGCCAGCCA CCCTGCTGCC CACCAAGGTC
    CACGACTGCA ACGGGCAGGT GCTGGCCCTG CCGAGCGAGT TCTTCCTGCG CATCGACAGC
    CACAAGGAGG ATCCGGCCGA GTTTGACGAC GATGTGGAGG ACAGCTGCTG CTCAAGGCTG
    CAGAAGGTGC TGGAGCCGCA TAAGCCGGAG TGCTGCCGGA GCCGCGAGCC CTGGGCCCTG
    TACCTCTTCT CCCCACAGAA CAGGTTCCGC ATCTCCTGCC AAAAGATCAT TGCTCACAAG
    ATGTTCGATC ATGTCGTCCT CGTCTTCATC TTCCTCAACT GCATCACCAT CGCCCTGGAG
    AGGCCCGACA TCGACCCCGG CAGCACTGAG CGCGTCTTCC TCAGCGTCTC CAACTACATC
    TTCACAGCGA TCTTCGTGGC GGAGATGATG GTGAAGGTGG TGGCCCTGGG TCTCATCTCG
    GGTGAGCATG CCTACCTGCA GAGCAGCTGG AACATACTGG ATGGGGTGCT GGTCCTGGTG
    TCCCTGGTGG ACATCATCGT GGCCATGGCC TCGGCTGGCG GCGCCAAGAT CCTGGGCATC
    CTGCGTGTGC TGCGCCTGCT GCGGACCCTG CGGCCGCTAA GGGTCATCAG CCGTGCGCCG
    GGACTCAAGC TGGTGGTGGA GACTCTGATA TCGTCCCTCA GGCCCATCGG GAACATCGTC
    CTCATCTGCT GCGCCTTCTT CATCATCTTC GGCATCCTCG GGGTGCAGCT CTTCAAGGGG
    AAGTTTTACT ACTGCGAGGG CGCCGACACC AGGAACATTT CCACTAAGGC CGAGTGCAGG
    GCCGCGCACT ACCGCTGGGT GCGACGCAAG TACAACTTCG ACAACCTGGG CCAGGCGCTG
    ATGTCGCTGT TCGTGCTCTC GTCCAAGGAC GGCTGGGTGA ATATCATGTA CGACGGGCTG
    GACGCCGTGG GCGTGGACCA GCAGCCCGTG CCCAACCACA ACCCCTGGAT GCTGCTCTAC
    TTCATCTCCT TCCTGCTCAT CGTCAGCTTC TTCGTGCTCA ACATGTTCGT GGGCGTCGTG
    GTGGAGAACT TCCACAAGTG CCGGCAGCAC CAGGAGGCCG AGGAGGCGCG GCGGCGCGAG
    GAAAAGCGGC AGCGTCGCCT GGAGAGGAAG CGCAGGAGCA CTTTCCCTAA CCCAGAGGCC
    CAGCGACGGC CCTACTATGC GGACTACTCG CCCACCCGCC GCTCCATCCA CACTCTGTGC
    ACCAGCCACT ACCTGGACCT CTTCATCACC TTCATCATTG GCGTCAACGT CATCACCATG
    TCCATGGAGC ACTACAACCA GCCCAAGGCT CTGGACGAGG CCCTCAAGTA CTGCAATTAC
    GTGTTCACCA TCGTCTTTGT CGTCGAGGCC GTGCTGAAGC TGGTGGCCTT TGGGTTCCGG
    AGGTTCTTCA AGGACAGGTG GAACCAGCTG GACCTGGCCA TCGTTCTGCT GTCCATCATG
    GGCATCACGT TGGAGGAGAT CGAGATGAAT GCAGCGCTGC CCATCAACCC CACCATCATC
    CGAATCATGC GTGTCCTCCG TATCGCCCGC GTCCTGAAGC TGCTCAAGAT GGCCACGGGC
    ATGCGGGCCC TGCTGGACAC GGTGGTCCAG GCGCTGCCCC AGGTAGGGAA CCTCGGCCTG
    CTTTTCATGC TCCTGTTTTT TATCTATGCT GCCCTGGGAG TGGAGCTGTT CGGGAGGCTA
    GAATGCAGTG AGGACAACCC CTGCGAGGGC CTGAGCAGAC ACGCCACCTT CTCTAACTTC
    GGCATGGCCT TCCTCACGCT GTTCCGCGTG TCCACCGGGG ACAACTGGAA CGGGATCATG
    AAGGACACGC TGCGCGAGTG CGCCCGCGAG GACAAGCACT GCCTCACCTA CCTGCCGGCC
    ATCTCGCCCA TCTACTTCGT CACCTTCGTG CTGGTGGCCC AGTTCGTGCT TGTCAACGTG
    GTGGTGGCCG TGCTCATGAA GCACCTGGAG GAGAGCAACA AGGAGGCCCG CGAGGACGCC
    GAGCTGGACG CAGAGCTTGA GATGGAAATG GCGCAGGGGT CCCCCGCCCG TCCCAGGCCG
    GCGGCCCCTC AGAGCCCCGG CGCCTCGCCG GACTCCCCCA ACGTGCTGGT CGTGCGCAAG
    GTGTCGGTGT CCAGGATGCT ATCCCTGCCC AACGACAGCT ACATGTTCCG GCCCGTGGCG
    CCTGCGGCAG CCGCTCACCC CCACCCGCTA CAGGAGGTGG AGATGGAGAC CTACGCGGGC
    GCGGCCGCCG GCCAGGTCAC CGCTGCCCAC TCGCCGCCTG TGGAGTCCTG CGCCTCCCTC
    CAGGTTCCGT CCGCCGTGTC CTCCCCGTCC AGGGGCGGCG ACACCCTCCG TGCCCTGCCC
    CCACGGGGTG CAGCCCGGTC GCCTAATGTC GGCCGACTTC TCTGCAGACA GGAGGCCGTC
    CACACGGAGT CCCTGGAAGG GCATGTCGAT GGCACTGAGG ACAGCAGCCC ATTCTGGGGA
    GAGCCTGGCG GGAAGACCCC CGTGAGACAG GCATCCCTGG GGCCCTCCTT GCGGTCCCCA
    TCCCGGTCCC CGCGACCCTC CAGCATCCGC ACCCGCAAGC ACACTTCTGG ACAGCACTGC
    ATCTCCAGCC GGCCTCCTGC CCCAGGCGGG GAGGAGTCCG AAGCCCCAGA CCCAGCTGAC
    GAGGAGGTCA GCCACATCAC CAGCTCTGCC CGCAGCCCTT TGGCCTCGCC CACTGCCTGT
    GGGATCGTGG GCGGCGAGCC AGACGTGCAC AGGCTCTACA GCGTGGACGC CCGGGGCTTC
    CTGGACCAGC CGGGCCGGGT GGATGAGCAG AGGCGGCCCT TGGGGGAGCC AGGAGTTGGG
    GACAGCCGTC CAGAGGCCGG GGAGGCGAAG CCCCGGGTCC TGGAGGCCGA GCTGGCCCTA
    GGGGCGCGCA GGAAGAAGAA GATGAGCCCC CCCTGCATTT CCATAGAGCC CCCCGCTGAG
    GACGAGGGTG CGGCCAGGGC CCCTGCGGCA GAAGGCGGCA GCACCACCCT GCGGCGGCGA
    ACCCCATCCT GCGAGGCTGT GCCCCATAGG GACCCCCTGG AGCCCGCAGA CAGCACAGGG
    CTGGACGCTG CTGCCAAGGG GGAGCGGCGG GGCCAGGGCC CCTGCCGTAC AGAACACCTG
    ACTGTCCCGA ACTTCACCTT TGAGCCACTG GACGTGGGGA GCCCCAGTGG GGACCTGTTC
    ACAGATGCGG GCCATGGGGC CACCCCAGAA CCCAGACCCT CCTCCTCAGG CACCGCGGCC
    CCTCCAGAAC CCCGGGAGAT GGAGTCCACA GTGCCCTCAG GAGACCCCCT AGAGGAGGGG
    CGGGGGGTGC ACCTCACTGT CCCGGAGAGC CCACCGAAGA GAGCGTCCCC CCCGGCTGTG
    CCAGTCCCGG GTGACGACGT GGATGAGCCC GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230682
    Clone ID Related Accession (Same CDS sequence) XM_024985429.1
    Accession Version XM_024985429.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1H isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGACCGAGG GCGTGCAGGC GGCCGACGAG GTCCGCGTGC CCCTGGGCGC GCCAGCCCCG 
    GGCCCGGCGG CGGCGGCGGC GTCCCCGGCG AGCCCTGGGG CCCCGGGGCC AGAGGCGGAG
    CGGGGGTCGG GGCCCAGTGC GTCGCCCCCC GAGAGTCCGG CGGCCGAGCG CGGCGCCGAG
    CTGGGCGCCG ACGAGGAGCA GCCCGTCCCG TACCCGGCGC TGGCGGCCAC CGTCTTCTTC
    TGTCTCGGGC AGACCACGCG GCCGCGCAGC TGGTGTCTCC GGCTGGTCTG TAACCCGTGG
    TTCGAGCATG TCAGCATGCT GGTCATCATG CTCAACTGCG TGACCCTGGG CATGTTCCGG
    CCCTGCGAGG ACGTCGAGTG CCGCTCGGAG CGCTGTGGCA TCCTCGAGGC CTTTGACGAC
    TTCATCTTCG CCTTCTTCGC GGTGGAGATG GTCATCAAGA TGATCGCCCT GGGGCTGTTT
    GGGCAGAAGT GCTACTTGGG TGACACGTGG AACAGGCTGG ACTTCTTCAT CGTCATGGCG
    GGTATGATGG AGTACTCCTT GGACGGACAC AACGTGAGCC TCTCGGCCAT CCGAACCGTG
    CGCGTGCTGC GGCCCCTTCG TGCCATCAAC CGTGTGCCCA GCATGCGGAT CCTGGTCACG
    CTGCTGCTGG ACACACTGCC CATGCTCGGC AACGTTCTCC TGCTCTGCTT CTTTGTCTTC
    TTCATCTTCG GCATCGTGGG CGTCCAGCTT TGGGCCGGCC TGCTGCGCAA CCGCTGCTTC
    CTGGACAGCA CCTTCGCCAG GAACAGCAAC CTCAGCTTCC TGCGGCCGTA CTACCAGCCG
    GAGGAGGGCG AAGAAAACCC GTTCATCTGC TCCTCCCGCC GGGACAACGG CATGCAGAAG
    TGCTCGCACA TCCCCAGCCG CCGCGAGCTG CGCGTGGAGT GCACGCTGGG CTGGGAGGCC
    TACGGGCAGC CGCAGGCCGA GGGCGCAGGT GGCACGGGTC ACCACACCTG CATCAACTGG
    AACCAGTACT ACAATGTGTG CCGCTCGGGC GACTCCAACC CGCACAACGG GGCCATCAGC
    TTCGACAACA TCGGCTACGC CTGGATCGCC ATCTTCCAGG TGATCACGCT GGAGGGCTGG
    GTGGACATCA TGTACTACGT CATGGACGCC CACTCTTTCT ACAACTTCAT CTACTTCATC
    TTGCTTATCA TCGTGGGCTC CTTCTTCATG ATCAACCTGT GCCTGGTGGT CATCGCCACG
    CAGTTCTCAG AGACGAAGCA GCGGGAGAAC CAGCTGATGC GGGAGCAGCG GGCCCGTTAT
    CTGTCCAATG ACAGCACGCT AGCCAGCTTC TCTGAGCCGG GCAGCTGCTA TGAGGAGCTT
    CTCCGGTACG TGGGCCACGT GTGCCGCAAG CTAAAGCGCC GTGGCCTGCG CCTCTACGCC
    CGCTGGCAGA GCCGCTGGCG CAAGAAGGTG GAGCCCGGTG GCGCCACGCA TGGCCAGGGC
    TCAGGGCGGC GGCCGCGGCG GGCAGGCGGG CGCGCTGCCT CCATCCACCA CCTCGTGTAC
    CACCACCACC ACCATCACCA CCACCACTAC CACTTCAGCC ACGGCAGCCC CCGCCGGCCA
    GGCCCCGAGC CAGGAGCCGG CGACACTAGG CTGGTGCGGG CCGGAGCGCC AGCCTCACCC
    GGGCGCGGGC CCCCTGACGC CGAGTCGGTG CACAGTGTGT ACCACGCTGA CTGCCACGTG
    GAGGGGCCAC AGGAGAGGGC GCGCGTGGCA CACGCCGCAG CCACTGCCGC TGCTGGGCTC
    AAGCTGGCCA CCGGGCTGGG AGCCATGAAC TACCCCACCA TCCTGCCCTC GGCTGCGGGC
    AGTGGCAAAA GCGGCCTCGG GCCCAAGGGG AAGCGGCCTG GTGGCCCCCC GGGAGCCGGG
    GGGCACAGCC CCCTGAGGCT GAGCAGCCCG GACCCCTGCG AGAAGATCCA GCACCTGGTT
    GGGGAGCATG GACTGGGCCA GGCCCCCAGC CGCCTGTCAG GCCTGAGTGT GCCCTGCCCC
    CTGCCCAGCC CCCAGGCGGG CACGCTGACC TGTGAGCTGA GCAGCTGCCC GTACTGCACC
    AGTGCCCTGG AGGGCCCCGA GCTGGAGTTC AGCGACTCAG ACAGTGGAGA CTCAGACAGC
    AATGGGGTCT ACGAGTTCAC GCAGGATGTG CGGCACGGGG ACCACCGCGA CCCCATGCAG
    TCGCCCCCCG TGGCAGAGGC GCCAGGCGTG GGCGGCACAC GGCGGAGGGG GCCGCAGCGG
    GCGGTGGCAG GCGAGCAGGG TGGGCTGGGC CACGTCTGGG CCTCCTTCAG TGGCAAGCTG
    CGCCGCATCG TGGACAGCAA GTACTTCAAC CGGGGCATCA TGGTGGCCAT CCTCACTAAC
    ACGCTGAGCA TGGGTGTCGA GTACCACGAG CAGCCCGATG AGCTGACCAA CGCCCTGGAG
    ATCAGCAACA TTGTGTTCAC AAGCATGTTT GCTCTGGAGA TGCTGTTGAA GCTGCTGGCC
    TGCGGCCCAC TGGGCTACAT CCGGAACCCT TACAACATCT TTGACGGCAT CATCGTAGTC
    ATCAGCGTGT GGGAGATCAT CGGGCAGGCG GACGGCGGGC TGTCGGTGCT GCGCACCTTC
    CGGCTGCTGC GGGTGCTCAA GCTGGTGCGC TTCATGCCCG CACTGCGGCG CCAGCTGGTG
    GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTTTTCATC
    TTCATCTTCA GCATCCTTGG CATGCATCTC TTTGGCTGCA AATTCAGTCT GAAAACAGAT
    ACCGGAGACA CAATCCCCGA CCGGAAGAAC TTTGATTCCC TACTGTGGGC CATCGTCACC
    GTGTTCCAGA TCCTCACCCA GGAGGACTGG AACGTCGTCC TGTACAACGG CATGGCCTCC
    ACGTCCTCCT GGGCCGCCCT GTACTTCGTG GCCCTCATGA CCTTTGGCAA CTACGTGCTC
    TTCAACCTGC TGGTGGCCAT CCTGGTGGAG GGTTTCCAGG CCGAGGGTGA TGCCACCAGG
    TCCGACACAG ATGAAGACAA GACATCCACC CACTTGGAGG AGGACTTGGA CAAGTTCCGA
    GACCTCAGGG CCACAGAGAT GAAGATGTAC TCGCTGGCAG TGACACCCAA CGGGCACCTG
    GAGGGCCGGG GCAGCCTGCC CCCTCCCCTT ATCATGCGCA CAGCGGCCAC GCCCATGCCC
    ACCCCCAAGA GCTCCCCACA CCTGGACGAG GCCCCTGGCC CCCTGGACTC GCGCCGTGGC
    AGCAGCAGCT CCATGGACCC TCAGCTAGGA GACCAGAAAT CTCTGTCCAG CCTCCGCAGC
    TCGCCCTGCG CCCCCTGGGG GCCCAACAGC GCCTGGAGCA GCCGGCGCTC CAGCTGGAAC
    AGCCTGGGCC GTGCACCCAG CCTCAAGCGC AGGAGCCAGT GTGGGGAGCG CGAGTCGCTG
    CTGTCCGGTG AGGGCAGGGG CAGCGGCAGC ACTGACGAGG AGGCCGAGGA TGGCCGGCCC
    GGGGCGGGGG CCTCGCCGGG TACGCGTGCC ACGCCGCTGC GGCGCACCGA GTCCCTGGAC
    CACCGTAGCA CGCTGGACCT GCGGCCCCCA CGGCCAGCCA CCCTGCTGCC CACCAAGGTC
    CACGACTGCA ACGGGCAGGT GCTGGCCCTG CCGAGCGAGT TCTTCCTGCG CATCGACAGC
    CACAAGGAGG ATCCGGCCGA GTTTGACGAC GATGTGGAGG ACAGCTGCTG CTCAAGGCTG
    CAGAAGGTGC TGGAGCCGCA TAAGCCGGAG TGCTGCCGGA GCCGCGAGCC CTGGGCCCTG
    TACCTCTTCT CCCCACAGAA CAGGTTCCGC ATCTCCTGCC AAAAGATCAT TGCTCACAAG
    ATGTTCGATC ATGTCGTCCT CGTCTTCATC TTCCTCAACT GCATCACCAT CGCCCTGGAG
    AGGCCCGACA TCGACCCCGG CAGCACTGAG CGCGTCTTCC TCAGCGTCTC CAACTACATC
    TTCACAGCGA TCTTCGTGGC GGAGATGATG GTGAAGGTGG TGGCCCTGGG TCTCATCTCG
    GGTGAGCATG CCTACCTGCA GAGCAGCTGG AACATACTGG ATGGGGTGCT GGTCCTGGTG
    TCCCTGGTGG ACATCATCGT GGCCATGGCC TCGGCTGGCG GCGCCAAGAT CCTGGGCATC
    CTGCGTGTGC TGCGCCTGCT GCGGACCCTG CGGCCGCTAA GGGTCATCAG CCGTGCGCCG
    GGACTCAAGC TGGTGGTGGA GACTCTGATA TCGTCCCTCA GGCCCATCGG GAACATCGTC
    CTCATCTGCT GCGCCTTCTT CATCATCTTC GGCATCCTCG GGGTGCAGCT CTTCAAGGGG
    AAGTTTTACT ACTGCGAGGG CGCCGACACC AGGAACATTT CCACTAAGGC CGAGTGCAGG
    GCCGCGCACT ACCGCTGGGT GCGACGCAAG TACAACTTCG ACAACCTGGG CCAGGCGCTG
    ATGTCGCTGT TCGTGCTCTC GTCCAAGGAC GGCTGGGTGA ATATCATGTA CGACGGGCTG
    GACGCCGTGG GCGTGGACCA GCAGCCCGTG CCCAACCACA ACCCCTGGAT GCTGCTCTAC
    TTCATCTCCT TCCTGCTCAT CGTCAGCTTC TTCGTGCTCA ACATGTTCGT GGGCGTCGTG
    GTGGAGAACT TCCACAAGTG CCGGCAGCAC CAGGAGGCCG AGGAGGCGCG GCGGCGCGAG
    GAAAAGCGGC AGCGTCGCCT GGAGAGGAAG CGCAGGAAGG CCCAGCGACG GCCCTACTAT
    GCGGACTACT CGCCCACCCG CCGCTCCATC CACACTCTGT GCACCAGCCA CTACCTGGAC
    CTCTTCATCA CCTTCATCAT TGGCGTCAAC GTCATCACCA TGTCCATGGA GCACTACAAC
    CAGCCCAAGG CTCTGGACGA GGCCCTCAAG TACTGCAATT ACGTGTTCAC CATCGTCTTT
    GTCGTCGAGG CCGTGCTGAA GCTGGTGGCC TTTGGGTTCC GGAGGTTCTT CAAGGACAGG
    TGGAACCAGC TGGACCTGGC CATCGTTCTG CTGTCCATCA TGGGCATCAC GTTGGAGGAG
    ATCGAGATGA ATGCAGCGCT GCCCATCAAC CCCACCATCA TCCGAATCAT GCGTGTCCTC
    CGTATCGCCC GCGTCCTGAA GCTGCTCAAG ATGGCCACGG GCATGCGGGC CCTGCTGGAC
    ACGGTGGTCC AGGCGCTGCC CCAGGTAGGG AACCTCGGCC TGCTTTTCAT GCTCCTGTTT
    TTTATCTATG CTGCCCTGGG AGTGGAGCTG TTCGGGAGGC TAGAATGCAG TGAGGACAAC
    CCCTGCGAGG GCCTGAGCAG ACACGCCACC TTCTCTAACT TCGGCATGGC CTTCCTCACG
    CTGTTCCGCG TGTCCACCGG GGACAACTGG AACGGGATCA TGAAGGACAC GCTGCGCGAG
    TGCGCCCGCG AGGACAAGCA CTGCCTCACC TACCTGCCGG CCATCTCGCC CATCTACTTC
    GTCACCTTCG TGCTGGTGGC CCAGTTCGTG CTTGTCAACG TGGTGGTGGC CGTGCTCATG
    AAGCACCTGG AGGAGAGCAA CAAGGAGGCC CGCGAGGACG CCGAGCTGGA CGCAGAGCTT
    GAGATGGAAA TGGCGCAGGG GTCCCCCGCC CGTCCCAGGC CGGCGGCCCC TCAGAGCCCC
    GGCGCCTCGC CGGACTCCCC CAACGTGCTG GTCGTGCGCA AGGTGTCGGT GTCCAGGATG
    CTATCCCTGC CCAACGACAG CTACATGTTC CGGCCCGTGG CGCCTGCGGC AGCCGCTCAC
    CCCCACCCGC TACAGGAGGT GGAGATGGAG ACCTACGCGG GCGCGGCCGC CGGCCAGGTC
    ACCGCTGCCC ACTCGCCGCC TGTGGAGTCC TGCGCCTCCC TCCAGGTTCC GTCCGCCGTG
    TCCTCCCCGT CCAGGGGCGG CGACACCCTC CGTGCCCTGC CCCCACGGGG TGCAGCCCGG
    TCGCCTAATG TCGGCCGACT TCTCTGCAGA CAGGAGGCCG TCCACACGGA GTCCCTGGAA
    GGGCATGTCG ATGGCACTGA GGACAGCAGC CCATTCTGGG GAGAGCCTGG CGGGAAGACC
    CCCGTGAGAC AGGCATCCCT GGGGCCCTCC TTGCGGTCCC CATCCCGGTC CCCGCGACCC
    TCCAGCATCC GCACCCGCAA GCACACTTCT GGACAGCACT GCATCTCCAG CCGGCCTCCT
    GCCCCAGGCG GGGAGGAGTC CGAAGCCCCA GACCCAGCTG ACGAGGAGGT CAGCCACATC
    ACCAGCTCTG CCCGCAGCCC TTTGGCCTCG CCCACTGCCT GTGGGATCGT GGGCGGCGAG
    CCAGACGTGC ACAGGCTCTA CAGCGTGGAC GCCCGGGGCT TCCTGGACCA GCCGGGCCGG
    GTGGATGAGC AGAGGCGGCC CTTGGGGGAG CCAGGAGTTG GGGACAGCCG TCCAGAGGCC
    GGGGAGGCGA AGCCCCGGGT CCTGGAGGCC GAGCTGGCCC TAGGGGCGCG CAGGAAGAAG
    AAGATGAGCC CCCCCTGCAT TTCCATAGAG CCCCCCGCTG AGGACGAGGG TGCGGCCAGG
    GCCCCTGCGG CAGAAGGCGG CAGCACCACC CTGCGGCGGC GAACCCCATC CTGCGAGGCT
    GTGCCCCATA GGGACCCCCT GGAGCCCGCA GACAGCACAG GGCTGGACGC TGCTGCCAAG
    GGGGAGCGGC GGGGCCAGGG CCCCTGCCGT ACAGAACACC TGACTGTCCC GAACTTCACC
    TTTGAGCCAC TGGACGTGGG GAGCCCCAGT GGGGACCTGT TCACAGATGC GGGCCATGGG
    GCCACCCCAG AACCCAGACC CTCCTCCTCA GGCACCGCGG CCCCTCCAGA ACCCCGGGAG
    ATGGAGTCCA CAGTGCCCTC AGGAGACCCC CTAGAGGAGG GGCGGGGGGT GCACCTCACT
    GTCCCGGAGA GCCCACCGAA GAGAGCGTCC CCCCCGGCTG TGCCAGTCCC GGGTGACGAC
    GTGGATGAGC CCGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841197.1
    CDS150..7187
    Translation

    Target ORF information:

    RefSeq Version XM_024985429.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 H (CACNA1H), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024985429.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGACCGAGG GCGTGCAGGC GGCCGACGAG GTCCGCGTGC CCCTGGGCGC GCCAGCCCCG 
    GGCCCGGCGG CGGCGGCGGC GTCCCCGGCG AGCCCTGGGG CCCCGGGGCC AGAGGCGGAG
    CGGGGGTCGG GGCCCAGTGC GTCGCCCCCC GAGAGTCCGG CGGCCGAGCG CGGCGCCGAG
    CTGGGCGCCG ACGAGGAGCA GCCCGTCCCG TACCCGGCGC TGGCGGCCAC CGTCTTCTTC
    TGTCTCGGGC AGACCACGCG GCCGCGCAGC TGGTGTCTCC GGCTGGTCTG TAACCCGTGG
    TTCGAGCATG TCAGCATGCT GGTCATCATG CTCAACTGCG TGACCCTGGG CATGTTCCGG
    CCCTGCGAGG ACGTCGAGTG CCGCTCGGAG CGCTGTGGCA TCCTCGAGGC CTTTGACGAC
    TTCATCTTCG CCTTCTTCGC GGTGGAGATG GTCATCAAGA TGATCGCCCT GGGGCTGTTT
    GGGCAGAAGT GCTACTTGGG TGACACGTGG AACAGGCTGG ACTTCTTCAT CGTCATGGCG
    GGTATGATGG AGTACTCCTT GGACGGACAC AACGTGAGCC TCTCGGCCAT CCGAACCGTG
    CGCGTGCTGC GGCCCCTTCG TGCCATCAAC CGTGTGCCCA GCATGCGGAT CCTGGTCACG
    CTGCTGCTGG ACACACTGCC CATGCTCGGC AACGTTCTCC TGCTCTGCTT CTTTGTCTTC
    TTCATCTTCG GCATCGTGGG CGTCCAGCTT TGGGCCGGCC TGCTGCGCAA CCGCTGCTTC
    CTGGACAGCA CCTTCGCCAG GAACAGCAAC CTCAGCTTCC TGCGGCCGTA CTACCAGCCG
    GAGGAGGGCG AAGAAAACCC GTTCATCTGC TCCTCCCGCC GGGACAACGG CATGCAGAAG
    TGCTCGCACA TCCCCAGCCG CCGCGAGCTG CGCGTGGAGT GCACGCTGGG CTGGGAGGCC
    TACGGGCAGC CGCAGGCCGA GGGCGCAGGT GGCACGGGTC ACCACACCTG CATCAACTGG
    AACCAGTACT ACAATGTGTG CCGCTCGGGC GACTCCAACC CGCACAACGG GGCCATCAGC
    TTCGACAACA TCGGCTACGC CTGGATCGCC ATCTTCCAGG TGATCACGCT GGAGGGCTGG
    GTGGACATCA TGTACTACGT CATGGACGCC CACTCTTTCT ACAACTTCAT CTACTTCATC
    TTGCTTATCA TCGTGGGCTC CTTCTTCATG ATCAACCTGT GCCTGGTGGT CATCGCCACG
    CAGTTCTCAG AGACGAAGCA GCGGGAGAAC CAGCTGATGC GGGAGCAGCG GGCCCGTTAT
    CTGTCCAATG ACAGCACGCT AGCCAGCTTC TCTGAGCCGG GCAGCTGCTA TGAGGAGCTT
    CTCCGGTACG TGGGCCACGT GTGCCGCAAG CTAAAGCGCC GTGGCCTGCG CCTCTACGCC
    CGCTGGCAGA GCCGCTGGCG CAAGAAGGTG GAGCCCGGTG GCGCCACGCA TGGCCAGGGC
    TCAGGGCGGC GGCCGCGGCG GGCAGGCGGG CGCGCTGCCT CCATCCACCA CCTCGTGTAC
    CACCACCACC ACCATCACCA CCACCACTAC CACTTCAGCC ACGGCAGCCC CCGCCGGCCA
    GGCCCCGAGC CAGGAGCCGG CGACACTAGG CTGGTGCGGG CCGGAGCGCC AGCCTCACCC
    GGGCGCGGGC CCCCTGACGC CGAGTCGGTG CACAGTGTGT ACCACGCTGA CTGCCACGTG
    GAGGGGCCAC AGGAGAGGGC GCGCGTGGCA CACGCCGCAG CCACTGCCGC TGCTGGGCTC
    AAGCTGGCCA CCGGGCTGGG AGCCATGAAC TACCCCACCA TCCTGCCCTC GGCTGCGGGC
    AGTGGCAAAA GCGGCCTCGG GCCCAAGGGG AAGCGGCCTG GTGGCCCCCC GGGAGCCGGG
    GGGCACAGCC CCCTGAGGCT GAGCAGCCCG GACCCCTGCG AGAAGATCCA GCACCTGGTT
    GGGGAGCATG GACTGGGCCA GGCCCCCAGC CGCCTGTCAG GCCTGAGTGT GCCCTGCCCC
    CTGCCCAGCC CCCAGGCGGG CACGCTGACC TGTGAGCTGA GCAGCTGCCC GTACTGCACC
    AGTGCCCTGG AGGGCCCCGA GCTGGAGTTC AGCGACTCAG ACAGTGGAGA CTCAGACAGC
    AATGGGGTCT ACGAGTTCAC GCAGGATGTG CGGCACGGGG ACCACCGCGA CCCCATGCAG
    TCGCCCCCCG TGGCAGAGGC GCCAGGCGTG GGCGGCACAC GGCGGAGGGG GCCGCAGCGG
    GCGGTGGCAG GCGAGCAGGG TGGGCTGGGC CACGTCTGGG CCTCCTTCAG TGGCAAGCTG
    CGCCGCATCG TGGACAGCAA GTACTTCAAC CGGGGCATCA TGGTGGCCAT CCTCACTAAC
    ACGCTGAGCA TGGGTGTCGA GTACCACGAG CAGCCCGATG AGCTGACCAA CGCCCTGGAG
    ATCAGCAACA TTGTGTTCAC AAGCATGTTT GCTCTGGAGA TGCTGTTGAA GCTGCTGGCC
    TGCGGCCCAC TGGGCTACAT CCGGAACCCT TACAACATCT TTGACGGCAT CATCGTAGTC
    ATCAGCGTGT GGGAGATCAT CGGGCAGGCG GACGGCGGGC TGTCGGTGCT GCGCACCTTC
    CGGCTGCTGC GGGTGCTCAA GCTGGTGCGC TTCATGCCCG CACTGCGGCG CCAGCTGGTG
    GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTTTTCATC
    TTCATCTTCA GCATCCTTGG CATGCATCTC TTTGGCTGCA AATTCAGTCT GAAAACAGAT
    ACCGGAGACA CAATCCCCGA CCGGAAGAAC TTTGATTCCC TACTGTGGGC CATCGTCACC
    GTGTTCCAGA TCCTCACCCA GGAGGACTGG AACGTCGTCC TGTACAACGG CATGGCCTCC
    ACGTCCTCCT GGGCCGCCCT GTACTTCGTG GCCCTCATGA CCTTTGGCAA CTACGTGCTC
    TTCAACCTGC TGGTGGCCAT CCTGGTGGAG GGTTTCCAGG CCGAGGGTGA TGCCACCAGG
    TCCGACACAG ATGAAGACAA GACATCCACC CACTTGGAGG AGGACTTGGA CAAGTTCCGA
    GACCTCAGGG CCACAGAGAT GAAGATGTAC TCGCTGGCAG TGACACCCAA CGGGCACCTG
    GAGGGCCGGG GCAGCCTGCC CCCTCCCCTT ATCATGCGCA CAGCGGCCAC GCCCATGCCC
    ACCCCCAAGA GCTCCCCACA CCTGGACGAG GCCCCTGGCC CCCTGGACTC GCGCCGTGGC
    AGCAGCAGCT CCATGGACCC TCAGCTAGGA GACCAGAAAT CTCTGTCCAG CCTCCGCAGC
    TCGCCCTGCG CCCCCTGGGG GCCCAACAGC GCCTGGAGCA GCCGGCGCTC CAGCTGGAAC
    AGCCTGGGCC GTGCACCCAG CCTCAAGCGC AGGAGCCAGT GTGGGGAGCG CGAGTCGCTG
    CTGTCCGGTG AGGGCAGGGG CAGCGGCAGC ACTGACGAGG AGGCCGAGGA TGGCCGGCCC
    GGGGCGGGGG CCTCGCCGGG TACGCGTGCC ACGCCGCTGC GGCGCACCGA GTCCCTGGAC
    CACCGTAGCA CGCTGGACCT GCGGCCCCCA CGGCCAGCCA CCCTGCTGCC CACCAAGGTC
    CACGACTGCA ACGGGCAGGT GCTGGCCCTG CCGAGCGAGT TCTTCCTGCG CATCGACAGC
    CACAAGGAGG ATCCGGCCGA GTTTGACGAC GATGTGGAGG ACAGCTGCTG CTCAAGGCTG
    CAGAAGGTGC TGGAGCCGCA TAAGCCGGAG TGCTGCCGGA GCCGCGAGCC CTGGGCCCTG
    TACCTCTTCT CCCCACAGAA CAGGTTCCGC ATCTCCTGCC AAAAGATCAT TGCTCACAAG
    ATGTTCGATC ATGTCGTCCT CGTCTTCATC TTCCTCAACT GCATCACCAT CGCCCTGGAG
    AGGCCCGACA TCGACCCCGG CAGCACTGAG CGCGTCTTCC TCAGCGTCTC CAACTACATC
    TTCACAGCGA TCTTCGTGGC GGAGATGATG GTGAAGGTGG TGGCCCTGGG TCTCATCTCG
    GGTGAGCATG CCTACCTGCA GAGCAGCTGG AACATACTGG ATGGGGTGCT GGTCCTGGTG
    TCCCTGGTGG ACATCATCGT GGCCATGGCC TCGGCTGGCG GCGCCAAGAT CCTGGGCATC
    CTGCGTGTGC TGCGCCTGCT GCGGACCCTG CGGCCGCTAA GGGTCATCAG CCGTGCGCCG
    GGACTCAAGC TGGTGGTGGA GACTCTGATA TCGTCCCTCA GGCCCATCGG GAACATCGTC
    CTCATCTGCT GCGCCTTCTT CATCATCTTC GGCATCCTCG GGGTGCAGCT CTTCAAGGGG
    AAGTTTTACT ACTGCGAGGG CGCCGACACC AGGAACATTT CCACTAAGGC CGAGTGCAGG
    GCCGCGCACT ACCGCTGGGT GCGACGCAAG TACAACTTCG ACAACCTGGG CCAGGCGCTG
    ATGTCGCTGT TCGTGCTCTC GTCCAAGGAC GGCTGGGTGA ATATCATGTA CGACGGGCTG
    GACGCCGTGG GCGTGGACCA GCAGCCCGTG CCCAACCACA ACCCCTGGAT GCTGCTCTAC
    TTCATCTCCT TCCTGCTCAT CGTCAGCTTC TTCGTGCTCA ACATGTTCGT GGGCGTCGTG
    GTGGAGAACT TCCACAAGTG CCGGCAGCAC CAGGAGGCCG AGGAGGCGCG GCGGCGCGAG
    GAAAAGCGGC AGCGTCGCCT GGAGAGGAAG CGCAGGAAGG CCCAGCGACG GCCCTACTAT
    GCGGACTACT CGCCCACCCG CCGCTCCATC CACACTCTGT GCACCAGCCA CTACCTGGAC
    CTCTTCATCA CCTTCATCAT TGGCGTCAAC GTCATCACCA TGTCCATGGA GCACTACAAC
    CAGCCCAAGG CTCTGGACGA GGCCCTCAAG TACTGCAATT ACGTGTTCAC CATCGTCTTT
    GTCGTCGAGG CCGTGCTGAA GCTGGTGGCC TTTGGGTTCC GGAGGTTCTT CAAGGACAGG
    TGGAACCAGC TGGACCTGGC CATCGTTCTG CTGTCCATCA TGGGCATCAC GTTGGAGGAG
    ATCGAGATGA ATGCAGCGCT GCCCATCAAC CCCACCATCA TCCGAATCAT GCGTGTCCTC
    CGTATCGCCC GCGTCCTGAA GCTGCTCAAG ATGGCCACGG GCATGCGGGC CCTGCTGGAC
    ACGGTGGTCC AGGCGCTGCC CCAGGTAGGG AACCTCGGCC TGCTTTTCAT GCTCCTGTTT
    TTTATCTATG CTGCCCTGGG AGTGGAGCTG TTCGGGAGGC TAGAATGCAG TGAGGACAAC
    CCCTGCGAGG GCCTGAGCAG ACACGCCACC TTCTCTAACT TCGGCATGGC CTTCCTCACG
    CTGTTCCGCG TGTCCACCGG GGACAACTGG AACGGGATCA TGAAGGACAC GCTGCGCGAG
    TGCGCCCGCG AGGACAAGCA CTGCCTCACC TACCTGCCGG CCATCTCGCC CATCTACTTC
    GTCACCTTCG TGCTGGTGGC CCAGTTCGTG CTTGTCAACG TGGTGGTGGC CGTGCTCATG
    AAGCACCTGG AGGAGAGCAA CAAGGAGGCC CGCGAGGACG CCGAGCTGGA CGCAGAGCTT
    GAGATGGAAA TGGCGCAGGG GTCCCCCGCC CGTCCCAGGC CGGCGGCCCC TCAGAGCCCC
    GGCGCCTCGC CGGACTCCCC CAACGTGCTG GTCGTGCGCA AGGTGTCGGT GTCCAGGATG
    CTATCCCTGC CCAACGACAG CTACATGTTC CGGCCCGTGG CGCCTGCGGC AGCCGCTCAC
    CCCCACCCGC TACAGGAGGT GGAGATGGAG ACCTACGCGG GCGCGGCCGC CGGCCAGGTC
    ACCGCTGCCC ACTCGCCGCC TGTGGAGTCC TGCGCCTCCC TCCAGGTTCC GTCCGCCGTG
    TCCTCCCCGT CCAGGGGCGG CGACACCCTC CGTGCCCTGC CCCCACGGGG TGCAGCCCGG
    TCGCCTAATG TCGGCCGACT TCTCTGCAGA CAGGAGGCCG TCCACACGGA GTCCCTGGAA
    GGGCATGTCG ATGGCACTGA GGACAGCAGC CCATTCTGGG GAGAGCCTGG CGGGAAGACC
    CCCGTGAGAC AGGCATCCCT GGGGCCCTCC TTGCGGTCCC CATCCCGGTC CCCGCGACCC
    TCCAGCATCC GCACCCGCAA GCACACTTCT GGACAGCACT GCATCTCCAG CCGGCCTCCT
    GCCCCAGGCG GGGAGGAGTC CGAAGCCCCA GACCCAGCTG ACGAGGAGGT CAGCCACATC
    ACCAGCTCTG CCCGCAGCCC TTTGGCCTCG CCCACTGCCT GTGGGATCGT GGGCGGCGAG
    CCAGACGTGC ACAGGCTCTA CAGCGTGGAC GCCCGGGGCT TCCTGGACCA GCCGGGCCGG
    GTGGATGAGC AGAGGCGGCC CTTGGGGGAG CCAGGAGTTG GGGACAGCCG TCCAGAGGCC
    GGGGAGGCGA AGCCCCGGGT CCTGGAGGCC GAGCTGGCCC TAGGGGCGCG CAGGAAGAAG
    AAGATGAGCC CCCCCTGCAT TTCCATAGAG CCCCCCGCTG AGGACGAGGG TGCGGCCAGG
    GCCCCTGCGG CAGAAGGCGG CAGCACCACC CTGCGGCGGC GAACCCCATC CTGCGAGGCT
    GTGCCCCATA GGGACCCCCT GGAGCCCGCA GACAGCACAG GGCTGGACGC TGCTGCCAAG
    GGGGAGCGGC GGGGCCAGGG CCCCTGCCGT ACAGAACACC TGACTGTCCC GAACTTCACC
    TTTGAGCCAC TGGACGTGGG GAGCCCCAGT GGGGACCTGT TCACAGATGC GGGCCATGGG
    GCCACCCCAG AACCCAGACC CTCCTCCTCA GGCACCGCGG CCCCTCCAGA ACCCCGGGAG
    ATGGAGTCCA CAGTGCCCTC AGGAGACCCC CTAGAGGAGG GGCGGGGGGT GCACCTCACT
    GTCCCGGAGA GCCCACCGAA GAGAGCGTCC CCCCCGGCTG TGCCAGTCCC GGGTGACGAC
    GTGGATGAGC CCGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230683
    Clone ID Related Accession (Same CDS sequence) XM_024985430.1
    Accession Version XM_024985430.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7023bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1H isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGACCGAGG GCGTGCAGGC GGCCGACGAG GTCCGCGTGC CCCTGGGCGC GCCAGCCCCG 
    GGCCCGGCGG CGGCGGCGGC GTCCCCGGCG AGCCCTGGGG CCCCGGGGCC AGAGGCGGAG
    CGGGGGTCGG GGCCCAGTGC GTCGCCCCCC GAGAGTCCGG CGGCCGAGCG CGGCGCCGAG
    CTGGGCGCCG ACGAGGAGCA GCCCGTCCCG TACCCGGCGC TGGCGGCCAC CGTCTTCTTC
    TGTCTCGGGC AGACCACGCG GCCGCGCAGC TGGTGTCTCC GGCTGGTCTG TAACCCGTGG
    TTCGAGCATG TCAGCATGCT GGTCATCATG CTCAACTGCG TGACCCTGGG CATGTTCCGG
    CCCTGCGAGG ACGTCGAGTG CCGCTCGGAG CGCTGTGGCA TCCTCGAGGC CTTTGACGAC
    TTCATCTTCG CCTTCTTCGC GGTGGAGATG GTCATCAAGA TGATCGCCCT GGGGCTGTTT
    GGGCAGAAGT GCTACTTGGG TGACACGTGG AACAGGCTGG ACTTCTTCAT CGTCATGGCG
    GGTATGATGG AGTACTCCTT GGACGGACAC AACGTGAGCC TCTCGGCCAT CCGAACCGTG
    CGCGTGCTGC GGCCCCTTCG TGCCATCAAC CGTGTGCCCA GCATGCGGAT CCTGGTCACG
    CTGCTGCTGG ACACACTGCC CATGCTCGGC AACGTTCTCC TGCTCTGCTT CTTTGTCTTC
    TTCATCTTCG GCATCGTGGG CGTCCAGCTT TGGGCCGGCC TGCTGCGCAA CCGCTGCTTC
    CTGGACAGCA CCTTCGCCAG GAACAGCAAC CTCAGCTTCC TGCGGCCGTA CTACCAGCCG
    GAGGAGGGCG AAGAAAACCC GTTCATCTGC TCCTCCCGCC GGGACAACGG CATGCAGAAG
    TGCTCGCACA TCCCCAGCCG CCGCGAGCTG CGCGTGGAGT GCACGCTGGG CTGGGAGGCC
    TACGGGCAGC CGCAGGCCGA GGGCGCAGGT GGCACGGGTC ACCACACCTG CATCAACTGG
    AACCAGTACT ACAATGTGTG CCGCTCGGGC GACTCCAACC CGCACAACGG GGCCATCAGC
    TTCGACAACA TCGGCTACGC CTGGATCGCC ATCTTCCAGG TGATCACGCT GGAGGGCTGG
    GTGGACATCA TGTACTACGT CATGGACGCC CACTCTTTCT ACAACTTCAT CTACTTCATC
    TTGCTTATCA TCGTGGGCTC CTTCTTCATG ATCAACCTGT GCCTGGTGGT CATCGCCACG
    CAGTTCTCAG AGACGAAGCA GCGGGAGAAC CAGCTGATGC GGGAGCAGCG GGCCCGTTAT
    CTGTCCAATG ACAGCACGCT AGCCAGCTTC TCTGAGCCGG GCAGCTGCTA TGAGGAGCTT
    CTCCGGTACG TGGGCCACGT GTGCCGCAAG CTAAAGCGCC GTGGCCTGCG CCTCTACGCC
    CGCTGGCAGA GCCGCTGGCG CAAGAAGGTG GAGCCCGGTG GCGCCACGCA TGGCCAGGGC
    TCAGGGCGGC GGCCGCGGCG GGCAGGCGGG CGCGCTGCCT CCATCCACCA CCTCGTGTAC
    CACCACCACC ACCATCACCA CCACCACTAC CACTTCAGCC ACGGCAGCCC CCGCCGGCCA
    GGCCCCGAGC CAGGAGCCGG CGACACTAGG CTGGTGCGGG CCGGAGCGCC AGCCTCACCC
    GGGCGCGGGC CCCCTGACGC CGAGTCGGTG CACAGTGTGT ACCACGCTGA CTGCCACGTG
    GAGGGGCCAC AGGAGAGGGC GCGCGTGGCA CACGCCGCAG CCACTGCCGC TGCTGGGCTC
    AAGCTGGCCA CCGGGCTGGG AGCCATGAAC TACCCCACCA TCCTGCCCTC GGCTGCGGGC
    AGTGGCAAAA GCGGCCTCGG GCCCAAGGGG AAGCGGCCTG GTGGCCCCCC GGGAGCCGGG
    GGGCACAGCC CCCTGAGGCT GAGCAGCCCG GACCCCTGCG AGAAGATCCA GCACCTGGTT
    GGGGAGCATG GACTGGGCCA GGCCCCCAGC CGCCTGTCAG GCCTGAGTGT GCCCTGCCCC
    CTGCCCAGCC CCCAGGCGGG CACGCTGACC TGTGAGCTGA GCAGCTGCCC GTACTGCACC
    AGTGCCCTGG AGGGCCCCGA GCTGGAGTTC AGCGACTCAG ACAGTGGAGA CTCAGACAGC
    AATGGGGTCT ACGAGTTCAC GCAGGATGTG CGGCACGGGG ACCACCGCGA CCCCATGCAG
    TCGCCCCCCG TGGCAGAGGC GCCAGGCGTG GGCGGCACAC GGCGGAGGGG GCCGCAGCGG
    GCGGTGGCAG GCGAGCAGGG TGGGCTGGGC CACGTCTGGG CCTCCTTCAG TGGCAAGCTG
    CGCCGCATCG TGGACAGCAA GTACTTCAAC CGGGGCATCA TGGTGGCCAT CCTCACTAAC
    ACGCTGAGCA TGGGTGTCGA GTACCACGAG CAGCCCGATG AGCTGACCAA CGCCCTGGAG
    ATCAGCAACA TTGTGTTCAC AAGCATGTTT GCTCTGGAGA TGCTGTTGAA GCTGCTGGCC
    TGCGGCCCAC TGGGCTACAT CCGGAACCCT TACAACATCT TTGACGGCAT CATCGTAGTC
    ATCAGCGTGT GGGAGATCAT CGGGCAGGCG GACGGCGGGC TGTCGGTGCT GCGCACCTTC
    CGGCTGCTGC GGGTGCTCAA GCTGGTGCGC TTCATGCCCG CACTGCGGCG CCAGCTGGTG
    GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTTTTCATC
    TTCATCTTCA GCATCCTTGG CATGCATCTC TTTGGCTGCA AATTCAGTCT GAAAACAGAT
    ACCGGAGACA CAATCCCCGA CCGGAAGAAC TTTGATTCCC TACTGTGGGC CATCGTCACC
    GTGTTCCAGA TCCTCACCCA GGAGGACTGG AACGTCGTCC TGTACAACGG CATGGCCTCC
    ACGTCCTCCT GGGCCGCCCT GTACTTCGTG GCCCTCATGA CCTTTGGCAA CTACGTGCTC
    TTCAACCTGC TGGTGGCCAT CCTGGTGGAG GGTTTCCAGG CCGAGGGTGA TGCCACCAGG
    TCCGACACAG ATGAAGACAA GACATCCACC CACTTGGAGG AGGACTTGGA CAAGTTCCGA
    GACCTCAGGG CCACAGAGAT GAAGATGTAC TCGCTGGCAG TGACACCCAA CGGGCACCTG
    GAGGGCCGGG GCAGCCTGCC CCCTCCCCTT ATCATGCGCA CAGCGGCCAC GCCCATGCCC
    ACCCCCAAGA GCTCCCCACA CCTGGACGAG GCCCCTGGCC CCCTGGACTC GCGCCGTGGC
    AGCAGCAGCT CCATGGACCC TCAGCTAGGA GACCAGAAAT CTCTGTCCAG CCTCCGCAGC
    TCGCCCTGCG CCCCCTGGGG GCCCAACAGC GCCTGGAGCA GCCGGCGCTC CAGCTGGAAC
    AGCCTGGGCC GTGCACCCAG CCTCAAGCGC AGGAGCCAGT GTGGGGAGCG CGAGTCGCTG
    CTGTCCGGTG AGGGCAGGGG CAGCGGCAGC ACTGACGAGG AGGCCGAGGA TGGCCGGCCC
    GGGGCGGGGG CCTCGCCGGG TACGCGTGCC ACGCCGCTGC GGCGCACCGA GTCCCTGGAC
    CACCGTAGCA CGCTGGACCT GCGGCCCCCA CGGCCAGCCA CCCTGCTGCC CACCAAGGTC
    CACGACTGCA ACGGGCAGGT GCTGGCCCTG CCGAGCGAGT TCTTCCTGCG CATCGACAGC
    CACAAGGAGG ATCCGGCCGA GTTTGACGAC GATGTGGAGG ACAGCTGCTG CTCAAGGCTG
    CAGAAGGTGC TGGAGCCGCA TAAGCCGGAG TGCTGCCGGA GCCGCGAGCC CTGGGCCCTG
    TACCTCTTCT CCCCACAGAA CAGGTTCCGC ATCTCCTGCC AAAAGATCAT TGCTCACAAG
    ATGTTCGATC ATGTCGTCCT CGTCTTCATC TTCCTCAACT GCATCACCAT CGCCCTGGAG
    AGGCCCGACA TCGACCCCGG CAGCACTGAG CGCGTCTTCC TCAGCGTCTC CAACTACATC
    TTCACAGCGA TCTTCGTGGC GGAGATGATG GTGAAGGTGG TGGCCCTGGG TCTCATCTCG
    GGTGAGCATG CCTACCTGCA GAGCAGCTGG AACATACTGG ATGGGGTGCT GGTCCTGGTG
    TCCCTGGTGG ACATCATCGT GGCCATGGCC TCGGCTGGCG GCGCCAAGAT CCTGGGCATC
    CTGCGTGTGC TGCGCCTGCT GCGGACCCTG CGGCCGCTAA GGGTCATCAG CCGTGCGCCG
    GGACTCAAGC TGGTGGTGGA GACTCTGATA TCGTCCCTCA GGCCCATCGG GAACATCGTC
    CTCATCTGCT GCGCCTTCTT CATCATCTTC GGCATCCTCG GGGTGCAGCT CTTCAAGGGG
    AAGTTTTACT ACTGCGAGGG CGCCGACACC AGGAACATTT CCACTAAGGC CGAGTGCAGG
    GCCGCGCACT ACCGCTGGGT GCGACGCAAG TACAACTTCG ACAACCTGGG CCAGGACGGC
    TGGGTGAATA TCATGTACGA CGGGCTGGAC GCCGTGGGCG TGGACCAGCA GCCCGTGCCC
    AACCACAACC CCTGGATGCT GCTCTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC
    GTGCTCAACA TGTTCGTGGG CGTCGTGGTG GAGAACTTCC ACAAGTGCCG GCAGCACCAG
    GAGGCCGAGG AGGCGCGGCG GCGCGAGGAA AAGCGGCAGC GTCGCCTGGA GAGGAAGCGC
    AGGAGCACTT TCCCTAACCC AGAGGCCCAG CGACGGCCCT ACTATGCGGA CTACTCGCCC
    ACCCGCCGCT CCATCCACAC TCTGTGCACC AGCCACTACC TGGACCTCTT CATCACCTTC
    ATCATTGGCG TCAACGTCAT CACCATGTCC ATGGAGCACT ACAACCAGCC CAAGGCTCTG
    GACGAGGCCC TCAAGTACTG CAATTACGTG TTCACCATCG TCTTTGTCGT CGAGGCCGTG
    CTGAAGCTGG TGGCCTTTGG GTTCCGGAGG TTCTTCAAGG ACAGGTGGAA CCAGCTGGAC
    CTGGCCATCG TTCTGCTGTC CATCATGGGC ATCACGTTGG AGGAGATCGA GATGAATGCA
    GCGCTGCCCA TCAACCCCAC CATCATCCGA ATCATGCGTG TCCTCCGTAT CGCCCGCGTC
    CTGAAGCTGC TCAAGATGGC CACGGGCATG CGGGCCCTGC TGGACACGGT GGTCCAGGCG
    CTGCCCCAGG TAGGGAACCT CGGCCTGCTT TTCATGCTCC TGTTTTTTAT CTATGCTGCC
    CTGGGAGTGG AGCTGTTCGG GAGGCTAGAA TGCAGTGAGG ACAACCCCTG CGAGGGCCTG
    AGCAGACACG CCACCTTCTC TAACTTCGGC ATGGCCTTCC TCACGCTGTT CCGCGTGTCC
    ACCGGGGACA ACTGGAACGG GATCATGAAG GACACGCTGC GCGAGTGCGC CCGCGAGGAC
    AAGCACTGCC TCACCTACCT GCCGGCCATC TCGCCCATCT ACTTCGTCAC CTTCGTGCTG
    GTGGCCCAGT TCGTGCTTGT CAACGTGGTG GTGGCCGTGC TCATGAAGCA CCTGGAGGAG
    AGCAACAAGG AGGCCCGCGA GGACGCCGAG CTGGACGCAG AGCTTGAGAT GGAAATGGCG
    CAGGGGTCCC CCGCCCGTCC CAGGCCGGCG GCCCCTCAGA GCCCCGGCGC CTCGCCGGAC
    TCCCCCAACG TGCTGGTCGT GCGCAAGGTG TCGGTGTCCA GGATGCTATC CCTGCCCAAC
    GACAGCTACA TGTTCCGGCC CGTGGCGCCT GCGGCAGCCG CTCACCCCCA CCCGCTACAG
    GAGGTGGAGA TGGAGACCTA CGCGGGCGCG GCCGCCGGCC AGGTCACCGC TGCCCACTCG
    CCGCCTGTGG AGTCCTGCGC CTCCCTCCAG GTTCCGTCCG CCGTGTCCTC CCCGTCCAGG
    GGCGGCGACA CCCTCCGTGC CCTGCCCCCA CGGGGTGCAG CCCGGTCGCC TAATGTCGGC
    CGACTTCTCT GCAGACAGGA GGCCGTCCAC ACGGAGTCCC TGGAAGGGCA TGTCGATGGC
    ACTGAGGACA GCAGCCCATT CTGGGGAGAG CCTGGCGGGA AGACCCCCGT GAGACAGGCA
    TCCCTGGGGC CCTCCTTGCG GTCCCCATCC CGGTCCCCGC GACCCTCCAG CATCCGCACC
    CGCAAGCACA CTTCTGGACA GCACTGCATC TCCAGCCGGC CTCCTGCCCC AGGCGGGGAG
    GAGTCCGAAG CCCCAGACCC AGCTGACGAG GAGGTCAGCC ACATCACCAG CTCTGCCCGC
    AGCCCTTTGG CCTCGCCCAC TGCCTGTGGG ATCGTGGGCG GCGAGCCAGA CGTGCACAGG
    CTCTACAGCG TGGACGCCCG GGGCTTCCTG GACCAGCCGG GCCGGGTGGA TGAGCAGAGG
    CGGCCCTTGG GGGAGCCAGG AGTTGGGGAC AGCCGTCCAG AGGCCGGGGA GGCGAAGCCC
    CGGGTCCTGG AGGCCGAGCT GGCCCTAGGG GCGCGCAGGA AGAAGAAGAT GAGCCCCCCC
    TGCATTTCCA TAGAGCCCCC CGCTGAGGAC GAGGGTGCGG CCAGGGCCCC TGCGGCAGAA
    GGCGGCAGCA CCACCCTGCG GCGGCGAACC CCATCCTGCG AGGCTGTGCC CCATAGGGAC
    CCCCTGGAGC CCGCAGACAG CACAGGGCTG GACGCTGCTG CCAAGGGGGA GCGGCGGGGC
    CAGGGCCCCT GCCGTACAGA ACACCTGACT GTCCCGAACT TCACCTTTGA GCCACTGGAC
    GTGGGGAGCC CCAGTGGGGA CCTGTTCACA GATGCGGGCC ATGGGGCCAC CCCAGAACCC
    AGACCCTCCT CCTCAGGCAC CGCGGCCCCT CCAGAACCCC GGGAGATGGA GTCCACAGTG
    CCCTCAGGAG ACCCCCTAGA GGAGGGGCGG GGGGTGCACC TCACTGTCCC GGAGAGCCCA
    CCGAAGAGAG CGTCCCCCCC GGCTGTGCCA GTCCCGGGTG ACGACGTGGA TGAGCCCGTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841198.1
    CDS151..7173
    Translation

    Target ORF information:

    RefSeq Version XM_024985430.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 H (CACNA1H), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024985430.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGACCGAGG GCGTGCAGGC GGCCGACGAG GTCCGCGTGC CCCTGGGCGC GCCAGCCCCG 
    GGCCCGGCGG CGGCGGCGGC GTCCCCGGCG AGCCCTGGGG CCCCGGGGCC AGAGGCGGAG
    CGGGGGTCGG GGCCCAGTGC GTCGCCCCCC GAGAGTCCGG CGGCCGAGCG CGGCGCCGAG
    CTGGGCGCCG ACGAGGAGCA GCCCGTCCCG TACCCGGCGC TGGCGGCCAC CGTCTTCTTC
    TGTCTCGGGC AGACCACGCG GCCGCGCAGC TGGTGTCTCC GGCTGGTCTG TAACCCGTGG
    TTCGAGCATG TCAGCATGCT GGTCATCATG CTCAACTGCG TGACCCTGGG CATGTTCCGG
    CCCTGCGAGG ACGTCGAGTG CCGCTCGGAG CGCTGTGGCA TCCTCGAGGC CTTTGACGAC
    TTCATCTTCG CCTTCTTCGC GGTGGAGATG GTCATCAAGA TGATCGCCCT GGGGCTGTTT
    GGGCAGAAGT GCTACTTGGG TGACACGTGG AACAGGCTGG ACTTCTTCAT CGTCATGGCG
    GGTATGATGG AGTACTCCTT GGACGGACAC AACGTGAGCC TCTCGGCCAT CCGAACCGTG
    CGCGTGCTGC GGCCCCTTCG TGCCATCAAC CGTGTGCCCA GCATGCGGAT CCTGGTCACG
    CTGCTGCTGG ACACACTGCC CATGCTCGGC AACGTTCTCC TGCTCTGCTT CTTTGTCTTC
    TTCATCTTCG GCATCGTGGG CGTCCAGCTT TGGGCCGGCC TGCTGCGCAA CCGCTGCTTC
    CTGGACAGCA CCTTCGCCAG GAACAGCAAC CTCAGCTTCC TGCGGCCGTA CTACCAGCCG
    GAGGAGGGCG AAGAAAACCC GTTCATCTGC TCCTCCCGCC GGGACAACGG CATGCAGAAG
    TGCTCGCACA TCCCCAGCCG CCGCGAGCTG CGCGTGGAGT GCACGCTGGG CTGGGAGGCC
    TACGGGCAGC CGCAGGCCGA GGGCGCAGGT GGCACGGGTC ACCACACCTG CATCAACTGG
    AACCAGTACT ACAATGTGTG CCGCTCGGGC GACTCCAACC CGCACAACGG GGCCATCAGC
    TTCGACAACA TCGGCTACGC CTGGATCGCC ATCTTCCAGG TGATCACGCT GGAGGGCTGG
    GTGGACATCA TGTACTACGT CATGGACGCC CACTCTTTCT ACAACTTCAT CTACTTCATC
    TTGCTTATCA TCGTGGGCTC CTTCTTCATG ATCAACCTGT GCCTGGTGGT CATCGCCACG
    CAGTTCTCAG AGACGAAGCA GCGGGAGAAC CAGCTGATGC GGGAGCAGCG GGCCCGTTAT
    CTGTCCAATG ACAGCACGCT AGCCAGCTTC TCTGAGCCGG GCAGCTGCTA TGAGGAGCTT
    CTCCGGTACG TGGGCCACGT GTGCCGCAAG CTAAAGCGCC GTGGCCTGCG CCTCTACGCC
    CGCTGGCAGA GCCGCTGGCG CAAGAAGGTG GAGCCCGGTG GCGCCACGCA TGGCCAGGGC
    TCAGGGCGGC GGCCGCGGCG GGCAGGCGGG CGCGCTGCCT CCATCCACCA CCTCGTGTAC
    CACCACCACC ACCATCACCA CCACCACTAC CACTTCAGCC ACGGCAGCCC CCGCCGGCCA
    GGCCCCGAGC CAGGAGCCGG CGACACTAGG CTGGTGCGGG CCGGAGCGCC AGCCTCACCC
    GGGCGCGGGC CCCCTGACGC CGAGTCGGTG CACAGTGTGT ACCACGCTGA CTGCCACGTG
    GAGGGGCCAC AGGAGAGGGC GCGCGTGGCA CACGCCGCAG CCACTGCCGC TGCTGGGCTC
    AAGCTGGCCA CCGGGCTGGG AGCCATGAAC TACCCCACCA TCCTGCCCTC GGCTGCGGGC
    AGTGGCAAAA GCGGCCTCGG GCCCAAGGGG AAGCGGCCTG GTGGCCCCCC GGGAGCCGGG
    GGGCACAGCC CCCTGAGGCT GAGCAGCCCG GACCCCTGCG AGAAGATCCA GCACCTGGTT
    GGGGAGCATG GACTGGGCCA GGCCCCCAGC CGCCTGTCAG GCCTGAGTGT GCCCTGCCCC
    CTGCCCAGCC CCCAGGCGGG CACGCTGACC TGTGAGCTGA GCAGCTGCCC GTACTGCACC
    AGTGCCCTGG AGGGCCCCGA GCTGGAGTTC AGCGACTCAG ACAGTGGAGA CTCAGACAGC
    AATGGGGTCT ACGAGTTCAC GCAGGATGTG CGGCACGGGG ACCACCGCGA CCCCATGCAG
    TCGCCCCCCG TGGCAGAGGC GCCAGGCGTG GGCGGCACAC GGCGGAGGGG GCCGCAGCGG
    GCGGTGGCAG GCGAGCAGGG TGGGCTGGGC CACGTCTGGG CCTCCTTCAG TGGCAAGCTG
    CGCCGCATCG TGGACAGCAA GTACTTCAAC CGGGGCATCA TGGTGGCCAT CCTCACTAAC
    ACGCTGAGCA TGGGTGTCGA GTACCACGAG CAGCCCGATG AGCTGACCAA CGCCCTGGAG
    ATCAGCAACA TTGTGTTCAC AAGCATGTTT GCTCTGGAGA TGCTGTTGAA GCTGCTGGCC
    TGCGGCCCAC TGGGCTACAT CCGGAACCCT TACAACATCT TTGACGGCAT CATCGTAGTC
    ATCAGCGTGT GGGAGATCAT CGGGCAGGCG GACGGCGGGC TGTCGGTGCT GCGCACCTTC
    CGGCTGCTGC GGGTGCTCAA GCTGGTGCGC TTCATGCCCG CACTGCGGCG CCAGCTGGTG
    GTGCTCATGA AGACCATGGA CAACGTGGCC ACCTTCTGCA TGCTGCTCAT GCTTTTCATC
    TTCATCTTCA GCATCCTTGG CATGCATCTC TTTGGCTGCA AATTCAGTCT GAAAACAGAT
    ACCGGAGACA CAATCCCCGA CCGGAAGAAC TTTGATTCCC TACTGTGGGC CATCGTCACC
    GTGTTCCAGA TCCTCACCCA GGAGGACTGG AACGTCGTCC TGTACAACGG CATGGCCTCC
    ACGTCCTCCT GGGCCGCCCT GTACTTCGTG GCCCTCATGA CCTTTGGCAA CTACGTGCTC
    TTCAACCTGC TGGTGGCCAT CCTGGTGGAG GGTTTCCAGG CCGAGGGTGA TGCCACCAGG
    TCCGACACAG ATGAAGACAA GACATCCACC CACTTGGAGG AGGACTTGGA CAAGTTCCGA
    GACCTCAGGG CCACAGAGAT GAAGATGTAC TCGCTGGCAG TGACACCCAA CGGGCACCTG
    GAGGGCCGGG GCAGCCTGCC CCCTCCCCTT ATCATGCGCA CAGCGGCCAC GCCCATGCCC
    ACCCCCAAGA GCTCCCCACA CCTGGACGAG GCCCCTGGCC CCCTGGACTC GCGCCGTGGC
    AGCAGCAGCT CCATGGACCC TCAGCTAGGA GACCAGAAAT CTCTGTCCAG CCTCCGCAGC
    TCGCCCTGCG CCCCCTGGGG GCCCAACAGC GCCTGGAGCA GCCGGCGCTC CAGCTGGAAC
    AGCCTGGGCC GTGCACCCAG CCTCAAGCGC AGGAGCCAGT GTGGGGAGCG CGAGTCGCTG
    CTGTCCGGTG AGGGCAGGGG CAGCGGCAGC ACTGACGAGG AGGCCGAGGA TGGCCGGCCC
    GGGGCGGGGG CCTCGCCGGG TACGCGTGCC ACGCCGCTGC GGCGCACCGA GTCCCTGGAC
    CACCGTAGCA CGCTGGACCT GCGGCCCCCA CGGCCAGCCA CCCTGCTGCC CACCAAGGTC
    CACGACTGCA ACGGGCAGGT GCTGGCCCTG CCGAGCGAGT TCTTCCTGCG CATCGACAGC
    CACAAGGAGG ATCCGGCCGA GTTTGACGAC GATGTGGAGG ACAGCTGCTG CTCAAGGCTG
    CAGAAGGTGC TGGAGCCGCA TAAGCCGGAG TGCTGCCGGA GCCGCGAGCC CTGGGCCCTG
    TACCTCTTCT CCCCACAGAA CAGGTTCCGC ATCTCCTGCC AAAAGATCAT TGCTCACAAG
    ATGTTCGATC ATGTCGTCCT CGTCTTCATC TTCCTCAACT GCATCACCAT CGCCCTGGAG
    AGGCCCGACA TCGACCCCGG CAGCACTGAG CGCGTCTTCC TCAGCGTCTC CAACTACATC
    TTCACAGCGA TCTTCGTGGC GGAGATGATG GTGAAGGTGG TGGCCCTGGG TCTCATCTCG
    GGTGAGCATG CCTACCTGCA GAGCAGCTGG AACATACTGG ATGGGGTGCT GGTCCTGGTG
    TCCCTGGTGG ACATCATCGT GGCCATGGCC TCGGCTGGCG GCGCCAAGAT CCTGGGCATC
    CTGCGTGTGC TGCGCCTGCT GCGGACCCTG CGGCCGCTAA GGGTCATCAG CCGTGCGCCG
    GGACTCAAGC TGGTGGTGGA GACTCTGATA TCGTCCCTCA GGCCCATCGG GAACATCGTC
    CTCATCTGCT GCGCCTTCTT CATCATCTTC GGCATCCTCG GGGTGCAGCT CTTCAAGGGG
    AAGTTTTACT ACTGCGAGGG CGCCGACACC AGGAACATTT CCACTAAGGC CGAGTGCAGG
    GCCGCGCACT ACCGCTGGGT GCGACGCAAG TACAACTTCG ACAACCTGGG CCAGGACGGC
    TGGGTGAATA TCATGTACGA CGGGCTGGAC GCCGTGGGCG TGGACCAGCA GCCCGTGCCC
    AACCACAACC CCTGGATGCT GCTCTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC
    GTGCTCAACA TGTTCGTGGG CGTCGTGGTG GAGAACTTCC ACAAGTGCCG GCAGCACCAG
    GAGGCCGAGG AGGCGCGGCG GCGCGAGGAA AAGCGGCAGC GTCGCCTGGA GAGGAAGCGC
    AGGAGCACTT TCCCTAACCC AGAGGCCCAG CGACGGCCCT ACTATGCGGA CTACTCGCCC
    ACCCGCCGCT CCATCCACAC TCTGTGCACC AGCCACTACC TGGACCTCTT CATCACCTTC
    ATCATTGGCG TCAACGTCAT CACCATGTCC ATGGAGCACT ACAACCAGCC CAAGGCTCTG
    GACGAGGCCC TCAAGTACTG CAATTACGTG TTCACCATCG TCTTTGTCGT CGAGGCCGTG
    CTGAAGCTGG TGGCCTTTGG GTTCCGGAGG TTCTTCAAGG ACAGGTGGAA CCAGCTGGAC
    CTGGCCATCG TTCTGCTGTC CATCATGGGC ATCACGTTGG AGGAGATCGA GATGAATGCA
    GCGCTGCCCA TCAACCCCAC CATCATCCGA ATCATGCGTG TCCTCCGTAT CGCCCGCGTC
    CTGAAGCTGC TCAAGATGGC CACGGGCATG CGGGCCCTGC TGGACACGGT GGTCCAGGCG
    CTGCCCCAGG TAGGGAACCT CGGCCTGCTT TTCATGCTCC TGTTTTTTAT CTATGCTGCC
    CTGGGAGTGG AGCTGTTCGG GAGGCTAGAA TGCAGTGAGG ACAACCCCTG CGAGGGCCTG
    AGCAGACACG CCACCTTCTC TAACTTCGGC ATGGCCTTCC TCACGCTGTT CCGCGTGTCC
    ACCGGGGACA ACTGGAACGG GATCATGAAG GACACGCTGC GCGAGTGCGC CCGCGAGGAC
    AAGCACTGCC TCACCTACCT GCCGGCCATC TCGCCCATCT ACTTCGTCAC CTTCGTGCTG
    GTGGCCCAGT TCGTGCTTGT CAACGTGGTG GTGGCCGTGC TCATGAAGCA CCTGGAGGAG
    AGCAACAAGG AGGCCCGCGA GGACGCCGAG CTGGACGCAG AGCTTGAGAT GGAAATGGCG
    CAGGGGTCCC CCGCCCGTCC CAGGCCGGCG GCCCCTCAGA GCCCCGGCGC CTCGCCGGAC
    TCCCCCAACG TGCTGGTCGT GCGCAAGGTG TCGGTGTCCA GGATGCTATC CCTGCCCAAC
    GACAGCTACA TGTTCCGGCC CGTGGCGCCT GCGGCAGCCG CTCACCCCCA CCCGCTACAG
    GAGGTGGAGA TGGAGACCTA CGCGGGCGCG GCCGCCGGCC AGGTCACCGC TGCCCACTCG
    CCGCCTGTGG AGTCCTGCGC CTCCCTCCAG GTTCCGTCCG CCGTGTCCTC CCCGTCCAGG
    GGCGGCGACA CCCTCCGTGC CCTGCCCCCA CGGGGTGCAG CCCGGTCGCC TAATGTCGGC
    CGACTTCTCT GCAGACAGGA GGCCGTCCAC ACGGAGTCCC TGGAAGGGCA TGTCGATGGC
    ACTGAGGACA GCAGCCCATT CTGGGGAGAG CCTGGCGGGA AGACCCCCGT GAGACAGGCA
    TCCCTGGGGC CCTCCTTGCG GTCCCCATCC CGGTCCCCGC GACCCTCCAG CATCCGCACC
    CGCAAGCACA CTTCTGGACA GCACTGCATC TCCAGCCGGC CTCCTGCCCC AGGCGGGGAG
    GAGTCCGAAG CCCCAGACCC AGCTGACGAG GAGGTCAGCC ACATCACCAG CTCTGCCCGC
    AGCCCTTTGG CCTCGCCCAC TGCCTGTGGG ATCGTGGGCG GCGAGCCAGA CGTGCACAGG
    CTCTACAGCG TGGACGCCCG GGGCTTCCTG GACCAGCCGG GCCGGGTGGA TGAGCAGAGG
    CGGCCCTTGG GGGAGCCAGG AGTTGGGGAC AGCCGTCCAG AGGCCGGGGA GGCGAAGCCC
    CGGGTCCTGG AGGCCGAGCT GGCCCTAGGG GCGCGCAGGA AGAAGAAGAT GAGCCCCCCC
    TGCATTTCCA TAGAGCCCCC CGCTGAGGAC GAGGGTGCGG CCAGGGCCCC TGCGGCAGAA
    GGCGGCAGCA CCACCCTGCG GCGGCGAACC CCATCCTGCG AGGCTGTGCC CCATAGGGAC
    CCCCTGGAGC CCGCAGACAG CACAGGGCTG GACGCTGCTG CCAAGGGGGA GCGGCGGGGC
    CAGGGCCCCT GCCGTACAGA ACACCTGACT GTCCCGAACT TCACCTTTGA GCCACTGGAC
    GTGGGGAGCC CCAGTGGGGA CCTGTTCACA GATGCGGGCC ATGGGGCCAC CCCAGAACCC
    AGACCCTCCT CCTCAGGCAC CGCGGCCCCT CCAGAACCCC GGGAGATGGA GTCCACAGTG
    CCCTCAGGAG ACCCCCTAGA GGAGGGGCGG GGGGTGCACC TCACTGTCCC GGAGAGCCCA
    CCGAAGAGAG CGTCCCCCCC GGCTGTGCCA GTCCCGGGTG ACGACGTGGA TGAGCCCGTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230684
    Clone ID Related Accession (Same CDS sequence) XM_024985431.1
    Accession Version XM_024985431.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1H isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
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    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGACCGAGG GCGTGCAGGC GGCCGACGAG GTCCGCGTGC CCCTGGGCGC GCCAGCCCCG 
    GGCCCGGCGG CGGCGGCGGC GTCCCCGGCG AGCCCTGGGG CCCCGGGGCC AGAGGCGGAG
    CGGGGGTCGG GGCCCAGTGC GTCGCCCCCC GAGAGTCCGG CGGCCGAGCG CGGCGCCGAG
    CTGGGCGCCG ACGAGGAGCA GCCCGTCCCG TACCCGGCGC TGGCGGCCAC CGTCTTCTTC
    TGTCTCGGGC AGACCACGCG GCCGCGCAGC TGGTGTCTCC GGCTGGTCTG TAACCCGTGG
    TTCGAGCATG TCAGCATGCT GGTCATCATG CTCAACTGCG TGACCCTGGG CATGTTCCGG
    CCCTGCGAGG ACGTCGAGTG CCGCTCGGAG CGCTGTGGCA TCCTCGAGAT GGTCATCAAG
    ATGATCGCCC TGGGGCTGTT TGGGCAGAAG TGCTACTTGG GTGACACGTG GAACAGGCTG
    GACTTCTTCA TCGTCATGGC GGGTATGATG GAGTACTCCT TGGACGGACA CAACGTGAGC
    CTCTCGGCCA TCCGAACCGT GCGCGTGCTG CGGCCCCTTC GTGCCATCAA CCGTGTGCCC
    AGCATGCGGA TCCTGGTCAC GCTGCTGCTG GACACACTGC CCATGCTCGG CAACGTTCTC
    CTGCTCTGCT TCTTTGTCTT CTTCATCTTC GGCATCGTGG GCGTCCAGCT TTGGGCCGGC
    CTGCTGCGCA ACCGCTGCTT CCTGGACAGC ACCTTCGCCA GGAACAGCAA CCTCAGCTTC
    CTGCGGCCGT ACTACCAGCC GGAGGAGGGC GAAGAAAACC CGTTCATCTG CTCCTCCCGC
    CGGGACAACG GCATGCAGAA GTGCTCGCAC ATCCCCAGCC GCCGCGAGCT GCGCGTGGAG
    TGCACGCTGG GCTGGGAGGC CTACGGGCAG CCGCAGGCCG AGGGCGCAGG TGGCACGGGT
    CACCACACCT GCATCAACTG GAACCAGTAC TACAATGTGT GCCGCTCGGG CGACTCCAAC
    CCGCACAACG GGGCCATCAG CTTCGACAAC ATCGGCTACG CCTGGATCGC CATCTTCCAG
    GTGATCACGC TGGAGGGCTG GGTGGACATC ATGTACTACG TCATGGACGC CCACTCTTTC
    TACAACTTCA TCTACTTCAT CTTGCTTATC ATCGTGGGCT CCTTCTTCAT GATCAACCTG
    TGCCTGGTGG TCATCGCCAC GCAGTTCTCA GAGACGAAGC AGCGGGAGAA CCAGCTGATG
    CGGGAGCAGC GGGCCCGTTA TCTGTCCAAT GACAGCACGC TAGCCAGCTT CTCTGAGCCG
    GGCAGCTGCT ATGAGGAGCT TCTCCGGTAC GTGGGCCACG TGTGCCGCAA GCTAAAGCGC
    CGTGGCCTGC GCCTCTACGC CCGCTGGCAG AGCCGCTGGC GCAAGAAGGT GGAGCCCGGT
    GGCGCCACGC ATGGCCAGGG CTCAGGGCGG CGGCCGCGGC GGGCAGGCGG GCGCGCTGCC
    TCCATCCACC ACCTCGTGTA CCACCACCAC CACCATCACC ACCACCACTA CCACTTCAGC
    CACGGCAGCC CCCGCCGGCC AGGCCCCGAG CCAGGAGCCG GCGACACTAG GCTGGTGCGG
    GCCGGAGCGC CAGCCTCACC CGGGCGCGGG CCCCCTGACG CCGAGTCGGT GCACAGTGTG
    TACCACGCTG ACTGCCACGT GGAGGGGCCA CAGGAGAGGG CGCGCGTGGC ACACGCCGCA
    GCCACTGCCG CTGCTGGGCT CAAGCTGGCC ACCGGGCTGG GAGCCATGAA CTACCCCACC
    ATCCTGCCCT CGGCTGCGGG CAGTGGCAAA AGCGGCCTCG GGCCCAAGGG GAAGCGGCCT
    GGTGGCCCCC CGGGAGCCGG GGGGCACAGC CCCCTGAGGC TGAGCAGCCC GGACCCCTGC
    GAGAAGATCC AGCACCTGGT TGGGGAGCAT GGACTGGGCC AGGCCCCCAG CCGCCTGTCA
    GGCCTGAGTG TGCCCTGCCC CCTGCCCAGC CCCCAGGCGG GCACGCTGAC CTGTGAGCTG
    AGCAGCTGCC CGTACTGCAC CAGTGCCCTG GAGGGCCCCG AGCTGGAGTT CAGCGACTCA
    GACAGTGGAG ACTCAGACAG CAATGGGGTC TACGAGTTCA CGCAGGATGT GCGGCACGGG
    GACCACCGCG ACCCCATGCA GTCGCCCCCC GTGGCAGAGG CGCCAGGCGT GGGCGGCACA
    CGGCGGAGGG GGCCGCAGCG GGCGGTGGCA GGCGAGCAGG GTGGGCTGGG CCACGTCTGG
    GCCTCCTTCA GTGGCAAGCT GCGCCGCATC GTGGACAGCA AGTACTTCAA CCGGGGCATC
    ATGGTGGCCA TCCTCACTAA CACGCTGAGC ATGGGTGTCG AGTACCACGA GCAGCCCGAT
    GAGCTGACCA ACGCCCTGGA GATCAGCAAC ATTGTGTTCA CAAGCATGTT TGCTCTGGAG
    ATGCTGTTGA AGCTGCTGGC CTGCGGCCCA CTGGGCTACA TCCGGAACCC TTACAACATC
    TTTGACGGCA TCATCGTAGT CATCAGCGTG TGGGAGATCA TCGGGCAGGC GGACGGCGGG
    CTGTCGGTGC TGCGCACCTT CCGGCTGCTG CGGGTGCTCA AGCTGGTGCG CTTCATGCCC
    GCACTGCGGC GCCAGCTGGT GGTGCTCATG AAGACCATGG ACAACGTGGC CACCTTCTGC
    ATGCTGCTCA TGCTTTTCAT CTTCATCTTC AGCATCCTTG GCATGCATCT CTTTGGCTGC
    AAATTCAGTC TGAAAACAGA TACCGGAGAC ACAATCCCCG ACCGGAAGAA CTTTGATTCC
    CTACTGTGGG CCATCGTCAC CGTGTTCCAG ATCCTCACCC AGGAGGACTG GAACGTCGTC
    CTGTACAACG GCATGGCCTC CACGTCCTCC TGGGCCGCCC TGTACTTCGT GGCCCTCATG
    ACCTTTGGCA ACTACGTGCT CTTCAACCTG CTGGTGGCCA TCCTGGTGGA GGGTTTCCAG
    GCCGAGGGTG ATGCCACCAG GTCCGACACA GATGAAGACA AGACATCCAC CCACTTGGAG
    GAGGACTTGG ACAAGTTCCG AGACCTCAGG GCCACAGAGA TGAAGATGTA CTCGCTGGCA
    GTGACACCCA ACGGGCACCT GGAGGGCCGG GGCAGCCTGC CCCCTCCCCT TATCATGCGC
    ACAGCGGCCA CGCCCATGCC CACCCCCAAG AGCTCCCCAC ACCTGGACGA GGCCCCTGGC
    CCCCTGGACT CGCGCCGTGG CAGCAGCAGC TCCATGGACC CTCAGCTAGG AGACCAGAAA
    TCTCTGTCCA GCCTCCGCAG CTCGCCCTGC GCCCCCTGGG GGCCCAACAG CGCCTGGAGC
    AGCCGGCGCT CCAGCTGGAA CAGCCTGGGC CGTGCACCCA GCCTCAAGCG CAGGAGCCAG
    TGTGGGGAGC GCGAGTCGCT GCTGTCCGGT GAGGGCAGGG GCAGCGGCAG CACTGACGAG
    GAGGCCGAGG ATGGCCGGCC CGGGGCGGGG GCCTCGCCGG GTACGCGTGC CACGCCGCTG
    CGGCGCACCG AGTCCCTGGA CCACCGTAGC ACGCTGGACC TGCGGCCCCC ACGGCCAGCC
    ACCCTGCTGC CCACCAAGGT CCACGACTGC AACGGGCAGG TGCTGGCCCT GCCGAGCGAG
    TTCTTCCTGC GCATCGACAG CCACAAGGAG GATCCGGCCG AGTTTGACGA CGATGTGGAG
    GACAGCTGCT GCTCAAGGCT GCAGAAGGTG CTGGAGCCGC ATAAGCCGGA GTGCTGCCGG
    AGCCGCGAGC CCTGGGCCCT GTACCTCTTC TCCCCACAGA ACAGGTTCCG CATCTCCTGC
    CAAAAGATCA TTGCTCACAA GATGTTCGAT CATGTCGTCC TCGTCTTCAT CTTCCTCAAC
    TGCATCACCA TCGCCCTGGA GAGGCCCGAC ATCGACCCCG GCAGCACTGA GCGCGTCTTC
    CTCAGCGTCT CCAACTACAT CTTCACAGCG ATCTTCGTGG CGGAGATGAT GGTGAAGGTG
    GTGGCCCTGG GTCTCATCTC GGGTGAGCAT GCCTACCTGC AGAGCAGCTG GAACATACTG
    GATGGGGTGC TGGTCCTGGT GTCCCTGGTG GACATCATCG TGGCCATGGC CTCGGCTGGC
    GGCGCCAAGA TCCTGGGCAT CCTGCGTGTG CTGCGCCTGC TGCGGACCCT GCGGCCGCTA
    AGGGTCATCA GCCGTGCGCC GGGACTCAAG CTGGTGGTGG AGACTCTGAT ATCGTCCCTC
    AGGCCCATCG GGAACATCGT CCTCATCTGC TGCGCCTTCT TCATCATCTT CGGCATCCTC
    GGGGTGCAGC TCTTCAAGGG GAAGTTTTAC TACTGCGAGG GCGCCGACAC CAGGAACATT
    TCCACTAAGG CCGAGTGCAG GGCCGCGCAC TACCGCTGGG TGCGACGCAA GTACAACTTC
    GACAACCTGG GCCAGGCGCT GATGTCGCTG TTCGTGCTCT CGTCCAAGGA CGGCTGGGTG
    AATATCATGT ACGACGGGCT GGACGCCGTG GGCGTGGACC AGCAGCCCGT GCCCAACCAC
    AACCCCTGGA TGCTGCTCTA CTTCATCTCC TTCCTGCTCA TCGTCAGCTT CTTCGTGCTC
    AACATGTTCG TGGGCGTCGT GGTGGAGAAC TTCCACAAGT GCCGGCAGCA CCAGGAGGCC
    GAGGAGGCGC GGCGGCGCGA GGAAAAGCGG CAGCGTCGCC TGGAGAGGAA GCGCAGGAGC
    ACTTTCCCTA ACCCAGAGGC CCAGCGACGG CCCTACTATG CGGACTACTC GCCCACCCGC
    CGCTCCATCC ACACTCTGTG CACCAGCCAC TACCTGGACC TCTTCATCAC CTTCATCATT
    GGCGTCAACG TCATCACCAT GTCCATGGAG CACTACAACC AGCCCAAGGC TCTGGACGAG
    GCCCTCAAGT ACTGCAATTA CGTGTTCACC ATCGTCTTTG TCGTCGAGGC CGTGCTGAAG
    CTGGTGGCCT TTGGGTTCCG GAGGTTCTTC AAGGACAGGT GGAACCAGCT GGACCTGGCC
    ATCGTTCTGC TGTCCATCAT GGGCATCACG TTGGAGGAGA TCGAGATGAA TGCAGCGCTG
    CCCATCAACC CCACCATCAT CCGAATCATG CGTGTCCTCC GTATCGCCCG CGTCCTGAAG
    CTGCTCAAGA TGGCCACGGG CATGCGGGCC CTGCTGGACA CGGTGGTCCA GGCGCTGCCC
    CAGGTAGGGA ACCTCGGCCT GCTTTTCATG CTCCTGTTTT TTATCTATGC TGCCCTGGGA
    GTGGAGCTGT TCGGGAGGCT AGAATGCAGT GAGGACAACC CCTGCGAGGG CCTGAGCAGA
    CACGCCACCT TCTCTAACTT CGGCATGGCC TTCCTCACGC TGTTCCGCGT GTCCACCGGG
    GACAACTGGA ACGGGATCAT GAAGGACACG CTGCGCGAGT GCGCCCGCGA GGACAAGCAC
    TGCCTCACCT ACCTGCCGGC CATCTCGCCC ATCTACTTCG TCACCTTCGT GCTGGTGGCC
    CAGTTCGTGC TTGTCAACGT GGTGGTGGCC GTGCTCATGA AGCACCTGGA GGAGAGCAAC
    AAGGAGGCCC GCGAGGACGC CGAGCTGGAC GCAGAGCTTG AGATGGAAAT GGCGCAGGGG
    TCCCCCGCCC GTCCCAGGCC GGCGGCCCCT CAGAGCCCCG GCGCCTCGCC GGACTCCCCC
    AACGTGCTGG TCGTGCGCAA GGTGTCGGTG TCCAGGATGC TATCCCTGCC CAACGACAGC
    TACATGTTCC GGCCCGTGGC GCCTGCGGCA GCCGCTCACC CCCACCCGCT ACAGGAGGTG
    GAGATGGAGA CCTACGCGGG CGCGGCCGCC GGCCAGGTCA CCGCTGCCCA CTCGCCGCCT
    GTGGAGTCCT GCGCCTCCCT CCAGGTTCCG TCCGCCGTGT CCTCCCCGTC CAGGGGCGGC
    GACACCCTCC GTGCCCTGCC CCCACGGGGT GCAGCCCGGT CGCCTAATGT CGGCCGACTT
    CTCTGCAGAC AGGAGGCCGT CCACACGGAG TCCCTGGAAG GGCATGTCGA TGGCACTGAG
    GACAGCAGCC CATTCTGGGG AGAGCCTGGC GGGAAGACCC CCGTGAGACA GGCATCCCTG
    GGGCCCTCCT TGCGGTCCCC ATCCCGGTCC CCGCGACCCT CCAGCATCCG CACCCGCAAG
    CACACTTCTG GACAGCACTG CATCTCCAGC CGGCCTCCTG CCCCAGGCGG GGAGGAGTCC
    GAAGCCCCAG ACCCAGCTGA CGAGGAGGTC AGCCACATCA CCAGCTCTGC CCGCAGCCCT
    TTGGCCTCGC CCACTGCCTG TGGGATCGTG GGCGGCGAGC CAGACGTGCA CAGGCTCTAC
    AGCGTGGACG CCCGGGGCTT CCTGGACCAG CCGGGCCGGG TGGATGAGCA GAGGCGGCCC
    TTGGGGGAGC CAGGAGTTGG GGACAGCCGT CCAGAGGCCG GGGAGGCGAA GCCCCGGGTC
    CTGGAGGCCG AGCTGGCCCT AGGGGCGCGC AGGAAGAAGA AGATGAGCCC CCCCTGCATT
    TCCATAGAGC CCCCCGCTGA GGACGAGGGT GCGGCCAGGG CCCCTGCGGC AGAAGGCGGC
    AGCACCACCC TGCGGCGGCG AACCCCATCC TGCGAGGCTG TGCCCCATAG GGACCCCCTG
    GAGCCCGCAG ACAGCACAGG GCTGGACGCT GCTGCCAAGG GGGAGCGGCG GGGCCAGGGC
    CCCTGCCGTA CAGAACACCT GACTGTCCCG AACTTCACCT TTGAGCCACT GGACGTGGGG
    AGCCCCAGTG GGGACCTGTT CACAGATGCG GGCCATGGGG CCACCCCAGA ACCCAGACCC
    TCCTCCTCAG GCACCGCGGC CCCTCCAGAA CCCCGGGAGA TGGAGTCCAC AGTGCCCTCA
    GGAGACCCCC TAGAGGAGGG GCGGGGGGTG CACCTCACTG TCCCGGAGAG CCCACCGAAG
    AGAGCGTCCC CCCCGGCTGT GCCAGTCCCG GGTGACGACG TGGATGAGCC CGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841199.1
    CDS151..7167
    Translation

    Target ORF information:

    RefSeq Version XM_024985431.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 H (CACNA1H), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024985431.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGACCGAGG GCGTGCAGGC GGCCGACGAG GTCCGCGTGC CCCTGGGCGC GCCAGCCCCG 
    GGCCCGGCGG CGGCGGCGGC GTCCCCGGCG AGCCCTGGGG CCCCGGGGCC AGAGGCGGAG
    CGGGGGTCGG GGCCCAGTGC GTCGCCCCCC GAGAGTCCGG CGGCCGAGCG CGGCGCCGAG
    CTGGGCGCCG ACGAGGAGCA GCCCGTCCCG TACCCGGCGC TGGCGGCCAC CGTCTTCTTC
    TGTCTCGGGC AGACCACGCG GCCGCGCAGC TGGTGTCTCC GGCTGGTCTG TAACCCGTGG
    TTCGAGCATG TCAGCATGCT GGTCATCATG CTCAACTGCG TGACCCTGGG CATGTTCCGG
    CCCTGCGAGG ACGTCGAGTG CCGCTCGGAG CGCTGTGGCA TCCTCGAGAT GGTCATCAAG
    ATGATCGCCC TGGGGCTGTT TGGGCAGAAG TGCTACTTGG GTGACACGTG GAACAGGCTG
    GACTTCTTCA TCGTCATGGC GGGTATGATG GAGTACTCCT TGGACGGACA CAACGTGAGC
    CTCTCGGCCA TCCGAACCGT GCGCGTGCTG CGGCCCCTTC GTGCCATCAA CCGTGTGCCC
    AGCATGCGGA TCCTGGTCAC GCTGCTGCTG GACACACTGC CCATGCTCGG CAACGTTCTC
    CTGCTCTGCT TCTTTGTCTT CTTCATCTTC GGCATCGTGG GCGTCCAGCT TTGGGCCGGC
    CTGCTGCGCA ACCGCTGCTT CCTGGACAGC ACCTTCGCCA GGAACAGCAA CCTCAGCTTC
    CTGCGGCCGT ACTACCAGCC GGAGGAGGGC GAAGAAAACC CGTTCATCTG CTCCTCCCGC
    CGGGACAACG GCATGCAGAA GTGCTCGCAC ATCCCCAGCC GCCGCGAGCT GCGCGTGGAG
    TGCACGCTGG GCTGGGAGGC CTACGGGCAG CCGCAGGCCG AGGGCGCAGG TGGCACGGGT
    CACCACACCT GCATCAACTG GAACCAGTAC TACAATGTGT GCCGCTCGGG CGACTCCAAC
    CCGCACAACG GGGCCATCAG CTTCGACAAC ATCGGCTACG CCTGGATCGC CATCTTCCAG
    GTGATCACGC TGGAGGGCTG GGTGGACATC ATGTACTACG TCATGGACGC CCACTCTTTC
    TACAACTTCA TCTACTTCAT CTTGCTTATC ATCGTGGGCT CCTTCTTCAT GATCAACCTG
    TGCCTGGTGG TCATCGCCAC GCAGTTCTCA GAGACGAAGC AGCGGGAGAA CCAGCTGATG
    CGGGAGCAGC GGGCCCGTTA TCTGTCCAAT GACAGCACGC TAGCCAGCTT CTCTGAGCCG
    GGCAGCTGCT ATGAGGAGCT TCTCCGGTAC GTGGGCCACG TGTGCCGCAA GCTAAAGCGC
    CGTGGCCTGC GCCTCTACGC CCGCTGGCAG AGCCGCTGGC GCAAGAAGGT GGAGCCCGGT
    GGCGCCACGC ATGGCCAGGG CTCAGGGCGG CGGCCGCGGC GGGCAGGCGG GCGCGCTGCC
    TCCATCCACC ACCTCGTGTA CCACCACCAC CACCATCACC ACCACCACTA CCACTTCAGC
    CACGGCAGCC CCCGCCGGCC AGGCCCCGAG CCAGGAGCCG GCGACACTAG GCTGGTGCGG
    GCCGGAGCGC CAGCCTCACC CGGGCGCGGG CCCCCTGACG CCGAGTCGGT GCACAGTGTG
    TACCACGCTG ACTGCCACGT GGAGGGGCCA CAGGAGAGGG CGCGCGTGGC ACACGCCGCA
    GCCACTGCCG CTGCTGGGCT CAAGCTGGCC ACCGGGCTGG GAGCCATGAA CTACCCCACC
    ATCCTGCCCT CGGCTGCGGG CAGTGGCAAA AGCGGCCTCG GGCCCAAGGG GAAGCGGCCT
    GGTGGCCCCC CGGGAGCCGG GGGGCACAGC CCCCTGAGGC TGAGCAGCCC GGACCCCTGC
    GAGAAGATCC AGCACCTGGT TGGGGAGCAT GGACTGGGCC AGGCCCCCAG CCGCCTGTCA
    GGCCTGAGTG TGCCCTGCCC CCTGCCCAGC CCCCAGGCGG GCACGCTGAC CTGTGAGCTG
    AGCAGCTGCC CGTACTGCAC CAGTGCCCTG GAGGGCCCCG AGCTGGAGTT CAGCGACTCA
    GACAGTGGAG ACTCAGACAG CAATGGGGTC TACGAGTTCA CGCAGGATGT GCGGCACGGG
    GACCACCGCG ACCCCATGCA GTCGCCCCCC GTGGCAGAGG CGCCAGGCGT GGGCGGCACA
    CGGCGGAGGG GGCCGCAGCG GGCGGTGGCA GGCGAGCAGG GTGGGCTGGG CCACGTCTGG
    GCCTCCTTCA GTGGCAAGCT GCGCCGCATC GTGGACAGCA AGTACTTCAA CCGGGGCATC
    ATGGTGGCCA TCCTCACTAA CACGCTGAGC ATGGGTGTCG AGTACCACGA GCAGCCCGAT
    GAGCTGACCA ACGCCCTGGA GATCAGCAAC ATTGTGTTCA CAAGCATGTT TGCTCTGGAG
    ATGCTGTTGA AGCTGCTGGC CTGCGGCCCA CTGGGCTACA TCCGGAACCC TTACAACATC
    TTTGACGGCA TCATCGTAGT CATCAGCGTG TGGGAGATCA TCGGGCAGGC GGACGGCGGG
    CTGTCGGTGC TGCGCACCTT CCGGCTGCTG CGGGTGCTCA AGCTGGTGCG CTTCATGCCC
    GCACTGCGGC GCCAGCTGGT GGTGCTCATG AAGACCATGG ACAACGTGGC CACCTTCTGC
    ATGCTGCTCA TGCTTTTCAT CTTCATCTTC AGCATCCTTG GCATGCATCT CTTTGGCTGC
    AAATTCAGTC TGAAAACAGA TACCGGAGAC ACAATCCCCG ACCGGAAGAA CTTTGATTCC
    CTACTGTGGG CCATCGTCAC CGTGTTCCAG ATCCTCACCC AGGAGGACTG GAACGTCGTC
    CTGTACAACG GCATGGCCTC CACGTCCTCC TGGGCCGCCC TGTACTTCGT GGCCCTCATG
    ACCTTTGGCA ACTACGTGCT CTTCAACCTG CTGGTGGCCA TCCTGGTGGA GGGTTTCCAG
    GCCGAGGGTG ATGCCACCAG GTCCGACACA GATGAAGACA AGACATCCAC CCACTTGGAG
    GAGGACTTGG ACAAGTTCCG AGACCTCAGG GCCACAGAGA TGAAGATGTA CTCGCTGGCA
    GTGACACCCA ACGGGCACCT GGAGGGCCGG GGCAGCCTGC CCCCTCCCCT TATCATGCGC
    ACAGCGGCCA CGCCCATGCC CACCCCCAAG AGCTCCCCAC ACCTGGACGA GGCCCCTGGC
    CCCCTGGACT CGCGCCGTGG CAGCAGCAGC TCCATGGACC CTCAGCTAGG AGACCAGAAA
    TCTCTGTCCA GCCTCCGCAG CTCGCCCTGC GCCCCCTGGG GGCCCAACAG CGCCTGGAGC
    AGCCGGCGCT CCAGCTGGAA CAGCCTGGGC CGTGCACCCA GCCTCAAGCG CAGGAGCCAG
    TGTGGGGAGC GCGAGTCGCT GCTGTCCGGT GAGGGCAGGG GCAGCGGCAG CACTGACGAG
    GAGGCCGAGG ATGGCCGGCC CGGGGCGGGG GCCTCGCCGG GTACGCGTGC CACGCCGCTG
    CGGCGCACCG AGTCCCTGGA CCACCGTAGC ACGCTGGACC TGCGGCCCCC ACGGCCAGCC
    ACCCTGCTGC CCACCAAGGT CCACGACTGC AACGGGCAGG TGCTGGCCCT GCCGAGCGAG
    TTCTTCCTGC GCATCGACAG CCACAAGGAG GATCCGGCCG AGTTTGACGA CGATGTGGAG
    GACAGCTGCT GCTCAAGGCT GCAGAAGGTG CTGGAGCCGC ATAAGCCGGA GTGCTGCCGG
    AGCCGCGAGC CCTGGGCCCT GTACCTCTTC TCCCCACAGA ACAGGTTCCG CATCTCCTGC
    CAAAAGATCA TTGCTCACAA GATGTTCGAT CATGTCGTCC TCGTCTTCAT CTTCCTCAAC
    TGCATCACCA TCGCCCTGGA GAGGCCCGAC ATCGACCCCG GCAGCACTGA GCGCGTCTTC
    CTCAGCGTCT CCAACTACAT CTTCACAGCG ATCTTCGTGG CGGAGATGAT GGTGAAGGTG
    GTGGCCCTGG GTCTCATCTC GGGTGAGCAT GCCTACCTGC AGAGCAGCTG GAACATACTG
    GATGGGGTGC TGGTCCTGGT GTCCCTGGTG GACATCATCG TGGCCATGGC CTCGGCTGGC
    GGCGCCAAGA TCCTGGGCAT CCTGCGTGTG CTGCGCCTGC TGCGGACCCT GCGGCCGCTA
    AGGGTCATCA GCCGTGCGCC GGGACTCAAG CTGGTGGTGG AGACTCTGAT ATCGTCCCTC
    AGGCCCATCG GGAACATCGT CCTCATCTGC TGCGCCTTCT TCATCATCTT CGGCATCCTC
    GGGGTGCAGC TCTTCAAGGG GAAGTTTTAC TACTGCGAGG GCGCCGACAC CAGGAACATT
    TCCACTAAGG CCGAGTGCAG GGCCGCGCAC TACCGCTGGG TGCGACGCAA GTACAACTTC
    GACAACCTGG GCCAGGCGCT GATGTCGCTG TTCGTGCTCT CGTCCAAGGA CGGCTGGGTG
    AATATCATGT ACGACGGGCT GGACGCCGTG GGCGTGGACC AGCAGCCCGT GCCCAACCAC
    AACCCCTGGA TGCTGCTCTA CTTCATCTCC TTCCTGCTCA TCGTCAGCTT CTTCGTGCTC
    AACATGTTCG TGGGCGTCGT GGTGGAGAAC TTCCACAAGT GCCGGCAGCA CCAGGAGGCC
    GAGGAGGCGC GGCGGCGCGA GGAAAAGCGG CAGCGTCGCC TGGAGAGGAA GCGCAGGAGC
    ACTTTCCCTA ACCCAGAGGC CCAGCGACGG CCCTACTATG CGGACTACTC GCCCACCCGC
    CGCTCCATCC ACACTCTGTG CACCAGCCAC TACCTGGACC TCTTCATCAC CTTCATCATT
    GGCGTCAACG TCATCACCAT GTCCATGGAG CACTACAACC AGCCCAAGGC TCTGGACGAG
    GCCCTCAAGT ACTGCAATTA CGTGTTCACC ATCGTCTTTG TCGTCGAGGC CGTGCTGAAG
    CTGGTGGCCT TTGGGTTCCG GAGGTTCTTC AAGGACAGGT GGAACCAGCT GGACCTGGCC
    ATCGTTCTGC TGTCCATCAT GGGCATCACG TTGGAGGAGA TCGAGATGAA TGCAGCGCTG
    CCCATCAACC CCACCATCAT CCGAATCATG CGTGTCCTCC GTATCGCCCG CGTCCTGAAG
    CTGCTCAAGA TGGCCACGGG CATGCGGGCC CTGCTGGACA CGGTGGTCCA GGCGCTGCCC
    CAGGTAGGGA ACCTCGGCCT GCTTTTCATG CTCCTGTTTT TTATCTATGC TGCCCTGGGA
    GTGGAGCTGT TCGGGAGGCT AGAATGCAGT GAGGACAACC CCTGCGAGGG CCTGAGCAGA
    CACGCCACCT TCTCTAACTT CGGCATGGCC TTCCTCACGC TGTTCCGCGT GTCCACCGGG
    GACAACTGGA ACGGGATCAT GAAGGACACG CTGCGCGAGT GCGCCCGCGA GGACAAGCAC
    TGCCTCACCT ACCTGCCGGC CATCTCGCCC ATCTACTTCG TCACCTTCGT GCTGGTGGCC
    CAGTTCGTGC TTGTCAACGT GGTGGTGGCC GTGCTCATGA AGCACCTGGA GGAGAGCAAC
    AAGGAGGCCC GCGAGGACGC CGAGCTGGAC GCAGAGCTTG AGATGGAAAT GGCGCAGGGG
    TCCCCCGCCC GTCCCAGGCC GGCGGCCCCT CAGAGCCCCG GCGCCTCGCC GGACTCCCCC
    AACGTGCTGG TCGTGCGCAA GGTGTCGGTG TCCAGGATGC TATCCCTGCC CAACGACAGC
    TACATGTTCC GGCCCGTGGC GCCTGCGGCA GCCGCTCACC CCCACCCGCT ACAGGAGGTG
    GAGATGGAGA CCTACGCGGG CGCGGCCGCC GGCCAGGTCA CCGCTGCCCA CTCGCCGCCT
    GTGGAGTCCT GCGCCTCCCT CCAGGTTCCG TCCGCCGTGT CCTCCCCGTC CAGGGGCGGC
    GACACCCTCC GTGCCCTGCC CCCACGGGGT GCAGCCCGGT CGCCTAATGT CGGCCGACTT
    CTCTGCAGAC AGGAGGCCGT CCACACGGAG TCCCTGGAAG GGCATGTCGA TGGCACTGAG
    GACAGCAGCC CATTCTGGGG AGAGCCTGGC GGGAAGACCC CCGTGAGACA GGCATCCCTG
    GGGCCCTCCT TGCGGTCCCC ATCCCGGTCC CCGCGACCCT CCAGCATCCG CACCCGCAAG
    CACACTTCTG GACAGCACTG CATCTCCAGC CGGCCTCCTG CCCCAGGCGG GGAGGAGTCC
    GAAGCCCCAG ACCCAGCTGA CGAGGAGGTC AGCCACATCA CCAGCTCTGC CCGCAGCCCT
    TTGGCCTCGC CCACTGCCTG TGGGATCGTG GGCGGCGAGC CAGACGTGCA CAGGCTCTAC
    AGCGTGGACG CCCGGGGCTT CCTGGACCAG CCGGGCCGGG TGGATGAGCA GAGGCGGCCC
    TTGGGGGAGC CAGGAGTTGG GGACAGCCGT CCAGAGGCCG GGGAGGCGAA GCCCCGGGTC
    CTGGAGGCCG AGCTGGCCCT AGGGGCGCGC AGGAAGAAGA AGATGAGCCC CCCCTGCATT
    TCCATAGAGC CCCCCGCTGA GGACGAGGGT GCGGCCAGGG CCCCTGCGGC AGAAGGCGGC
    AGCACCACCC TGCGGCGGCG AACCCCATCC TGCGAGGCTG TGCCCCATAG GGACCCCCTG
    GAGCCCGCAG ACAGCACAGG GCTGGACGCT GCTGCCAAGG GGGAGCGGCG GGGCCAGGGC
    CCCTGCCGTA CAGAACACCT GACTGTCCCG AACTTCACCT TTGAGCCACT GGACGTGGGG
    AGCCCCAGTG GGGACCTGTT CACAGATGCG GGCCATGGGG CCACCCCAGA ACCCAGACCC
    TCCTCCTCAG GCACCGCGGC CCCTCCAGAA CCCCGGGAGA TGGAGTCCAC AGTGCCCTCA
    GGAGACCCCC TAGAGGAGGG GCGGGGGGTG CACCTCACTG TCCCGGAGAG CCCACCGAAG
    AGAGCGTCCC CCCCGGCTGT GCCAGTCCCG GGTGACGACG TGGATGAGCC CGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88470
    Clone ID Related Accession (Same CDS sequence) XM_015460299.1
    Accession Version XM_015460299.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7035bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent T-type calcium channel subunit alpha-1H isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCGCTCT CAGCGGGAAC GGAGGGGCAC CAAGGCTCCA GGGCACACAG CCCGCATGTG 
    CTGCAGGCGG TCCAGCCCAT CATCCTGTCC AGCCCCCTGG GCGCCGGGGG TGGGGCTAGG
    GGCCTCGGCC TGGCACTGCT GGCCCACCAC TTGGCTGCCT TCCGGAGGAG CAGAGGGGGA
    ACTTGGCGGT GCACTGTGGC TTCTGTGGAG CCGAGGCGCC ACCTGGTGGC CACTCTTAGA
    ATGTGCGTCC TCATTGAGGT GGCCGTCGGC ACCTGCTCCA CGTGGTTCGA GCATGTCAGC
    ATGCTGGTCA TCATGCTCAA CTGCGTGACC CTGGGCATGT TCCGGCCCTG CGAGGACGTC
    GAGTGCCGCT CGGAGCGCTG TGGCATCCTC GAGGCCTTTG ACGACTTCAT CTTCGCCTTC
    TTCGCGGTGG AGATGGTCAT CAAGATGATC GCCCTGGGGC TGTTTGGGCA GAAGTGCTAC
    TTGGGTGACA CGTGGAACAG GCTGGACTTC TTCATCGTCA TGGCGGGTAT GATGGAGTAC
    TCCTTGGACG GACACAACGT GAGCCTCTCG GCCATCCGAA CCGTGCGCGT GCTGCGGCCC
    CTTCGTGCCA TCAACCGTGT GCCCAGCATG CGGATCCTGG TCACGCTGCT GCTGGACACA
    CTGCCCATGC TCGGCAACGT TCTCCTGCTC TGCTTCTTTG TCTTCTTCAT CTTCGGCATC
    GTGGGCGTCC AGCTTTGGGC CGGCCTGCTG CGCAACCGCT GCTTCCTGGA CAGCACCTTC
    GCCAGGAACA GCAACCTCAG CTTCCTGCGG CCGTACTACC AGCCGGAGGA GGGCGAAGAA
    AACCCGTTCA TCTGCTCCTC CCGCCGGGAC AACGGCATGC AGAAGTGCTC GCACATCCCC
    AGCCGCCGCG AGCTGCGCGT GGAGTGCACG CTGGGCTGGG AGGCCTACGG GCAGCCGCAG
    GCCGAGGGCG CAGGTGGCAC GGGTCACCAC ACCTGCATCA ACTGGAACCA GTACTACAAT
    GTGTGCCGCT CGGGCGACTC CAACCCGCAC AACGGGGCCA TCAGCTTCGA CAACATCGGC
    TACGCCTGGA TCGCCATCTT CCAGGTGATC ACGCTGGAGG GCTGGGTGGA CATCATGTAC
    TACGTCATGG ACGCCCACTC TTTCTACAAC TTCATCTACT TCATCTTGCT TATCATCGTG
    GGCTCCTTCT TCATGATCAA CCTGTGCCTG GTGGTCATCG CCACGCAGTT CTCAGAGACG
    AAGCAGCGGG AGAACCAGCT GATGCGGGAG CAGCGGGCCC GTTATCTGTC CAATGACAGC
    ACGCTAGCCA GCTTCTCTGA GCCGGGCAGC TGCTATGAGG AGCTTCTCCG GTACGTGGGC
    CACGTGTGCC GCAAGCTAAA GCGCCGTGGC CTGCGCCTCT ACGCCCGCTG GCAGAGCCGC
    TGGCGCAAGA AGGTGGAGCC CGGTGGCGCC ACGCATGGCC AGGGCTCAGG GCGGCGGCCG
    CGGCGGGCAG GCGGGCGCGC TGCCTCCATC CACCACCTCG TGTACCACCA CCACCACCAT
    CACCACCACC ACTACCACTT CAGCCACGGC AGCCCCCGCC GGCCAGGCCC CGAGCCAGGA
    GCCGGCGACA CTAGGCTGGT GCGGGCCGGA GCGCCAGCCT CACCCGGGCG CGGGCCCCCT
    GACGCCGAGT CGGTGCACAG TGTGTACCAC GCTGACTGCC ACGTGGAGGG GCCACAGGAG
    AGGGCGCGCG TGGCACACGC CGCAGCCACT GCCGCTGCTG GGCTCAAGCT GGCCACCGGG
    CTGGGAGCCA TGAACTACCC CACCATCCTG CCCTCGGCTG CGGGCAGTGG CAAAAGCGGC
    CTCGGGCCCA AGGGGAAGCG GCCTGGTGGC CCCCCGGGAG CCGGGGGGCA CAGCCCCCTG
    AGGCTGAGCA GCCCGGACCC CTGCGAGAAG ATCCAGCACC TGGTTGGGGA GCATGGACTG
    GGCCAGGCCC CCAGCCGCCT GTCAGGCCTG AGTGTGCCCT GCCCCCTGCC CAGCCCCCAG
    GCGGGCACGC TGACCTGTGA GCTGAGCAGC TGCCCGTACT GCACCAGTGC CCTGGAGGGC
    CCCGAGCTGG AGTTCAGCGA CTCAGACAGT GGAGACTCAG ACAGCAATGG GGTCTACGAG
    TTCACGCAGG ATGTGCGGCA CGGGGACCAC CGCGACCCCA TGCAGTCGCC CCCCGTGGCA
    GAGGCGCCAG GCGTGGGCGG CACACGGCGG AGGGGGCCGC AGCGGGCGGT GGCAGGCGAG
    CAGGGTGGGC TGGGCCACGT CTGGGCCTCC TTCAGTGGCA AGCTGCGCCG CATCGTGGAC
    AGCAAGTACT TCAACCGGGG CATCATGGTG GCCATCCTCA CTAACACGCT GAGCATGGGT
    GTCGAGTACC ACGAGCAGCC CGATGACCGA ACCTGCTCCC AGGTGGGGTT GAGCAGAGGC
    CGAGGTGGCT CCAGGCTGTC GTTCCTGCCT CTGGGAAGAG AGTGTCCCAG AATCAGGGCG
    GGAGTAGGCC ATCCTAACCC CGCCCACCCC ACCCCACCCC CGACCCCCAG CGTGTGGGAG
    ATCATCGGGC AGGCGGACGG CGGGCTGTCG GTGCTGCGCA CCTTCCGGCT GCTGCGGGTG
    CTCAAGCTGG TGCGCTTCAT GCCCGCACTG CGGCGCCAGC TGGTGGTGCT CATGAAGACC
    ATGGACAACG TGGCCACCTT CTGCATGCTG CTCATGCTTT TCATCTTCAT CTTCAGCATC
    CTTGGCATGC ATCTCTTTGG CTGCAAATTC AGTCTGAAAA CAGATACCGG AGACACAATC
    CCCGACCGGA AGAACTTTGA TTCCCTACTG TGGGCCATCG TCACCGTGTT CCAGATCCTC
    ACCCAGGAGG ACTGGAACGT CGTCCTGTAC AACGGCATGG CCTCCACGTC CTCCTGGGCC
    GCCCTGTACT TCGTGGCCCT CATGACCTTT GGCAACTACG TGCTCTTCAA CCTGCTGGTG
    GCCATCCTGG TGGAGGGTTT CCAGGCCGAG GGTGATGCCA CCAGGTCCGA CACAGATGAA
    GACAAGACAT CCACCCACTT GGAGGAGGAC TTGGACAAGT TCCGAGACCT CAGGGCCACA
    GAGATGAAGA TGTACTCGCT GGCAGTGACA CCCAACGGGC ACCTGGAGGG CCGGGGCAGC
    CTGCCCCCTC CCCTTATCAT GCGCACAGCG GCCACGCCCA TGCCCACCCC CAAGAGCTCC
    CCACACCTGG ACGAGGCCCC TGGCCCCCTG GACTCGCGCC GTGGCAGCAG CAGCTCCATG
    GACCCTCAGC TAGGAGACCA GAAATCTCTG TCCAGCCTCC GCAGCTCGCC CTGCGCCCCC
    TGGGGGCCCA ACAGCGCCTG GAGCAGCCGG CGCTCCAGCT GGAACAGCCT GGGCCGTGCA
    CCCAGCCTCA AGCGCAGGAG CCAGTGTGGG GAGCGCGAGT CGCTGCTGTC CGGTGAGGGC
    AGGGGCAGCG GCAGCACTGA CGAGGAGGCC GAGGATGGCC GGCCCGGGGC GGGGGCCTCG
    CCGGGTACGC GTGCCACGCC GCTGCGGCGC ACCGAGTCCC TGGACCACCG TAGCACGCTG
    GACCTGCGGC CCCCACGGCC AGCCACCCTG CTGCCCACCA AGGTCCACGA CTGCAACGGG
    CAGGTGCTGG CCCTGCCGAG CGAGTTCTTC CTGCGCATCG ACAGCCACAA GGAGGATCCG
    GCCGAGTTTG ACGACGATGT GGAGGACAGC TGCTGCTCAA GGCTGCAGAA GGTGCTGGAG
    CCGCATAAGC CGGAGTGCTG CCGGAGCCGC GAGCCCTGGG CCCTGTACCT CTTCTCCCCA
    CAGAACAGGT TCCGCATCTC CTGCCAAAAG ATCATTGCTC ACAAGATGTT CGATCATGTC
    GTCCTCGTCT TCATCTTCCT CAACTGCATC ACCATCGCCC TGGAGAGGCC CGACATCGAC
    CCGGCAGAGC GCGTCTTCCT CAGCGTCTCC AACTACATCT TCACAGCGAT CTTCGTGGCG
    GAGATGATGG TGAAGGTGGT GGCCCTGGGT CTCATCTCGG GTGAGCATGC CTACCTGCAG
    AGCAGCTGGA ACATACTGGA TGGGGTGCTG GTCCTGGTGT CCCTGGTGGA CATCATCGTG
    GCCATGGCCT CGGCTGGCGG CGCCAAGATC CTGGGCATCC TGCGTGTGCT GCGCCTGCTG
    CGGACCCTGC GGCCGCTAAG GGTCATCAGC CGTGCGCCGG GACTCAAGCT GGTGGTGGAG
    ACTCTGATAT CGTCCCTCAG GCCCATCGGG AACATCGTCC TCATCTGCTG CGCCTTCTTC
    ATCATCTTCG GCATCCTCGG GGTGCAGCTC TTCAAGGGGA AGTTTTACTA CTGCGAGGGC
    GCCGACACCA GGAACATTTC CACTAAGGCC GAGTGCAGGG CCGCGCACTA CCGCTGGGTG
    CGACGCAAGT ACAACTTCGA CAACCTGGGC CAGGCGCTGA TGTCGCTGTT CGTGCTCTCG
    TCCAAGGACG GCTGGGTGAA TATCATGTAC GACGGGCTGG ACGCCGTGGG CGTGGACCAG
    CAGCCCGTGC CCAACCACAA CCCCTGGATG CTGCTCTACT TCATCTCCTT CCTGCTCATC
    GTCAGCTTCT TCGTGCTCAA CATGTTCGTG GGCGTCGTGG TGGAGAACTT CCACAAGTGC
    CGGCAGCACC AGGAGGCCGA GGAGGCGCGG CGGCGCGAGG AAAAGCGGCA GCGTCGCCTG
    GAGAGGAAGC GCAGGAGCAC TTTCCCTAAC CCAGAGGCCC AGCGACGGCC CTACTATGCG
    GACTACTCGC CCACCCGCCG CTCCATCCAC ACTCTGTGCA CCAGCCACTA CCTGGACCTC
    TTCATCACCT TCATCATTGG CGTCAACGTC ATCACCATGT CCATGGAGCA CTACAACCAG
    CCCAAGGCTC TGGACGAGGC CCTCAAGTAC TGCAATTACG TGTTCACCAT CGTCTTTGTC
    GTCGAGGCCG TGCTGAAGCT GGTGGCCTTT GGGTTCCGGA GGTTCTTCAA GGACAGGTGG
    AACCAGCTGG ACCTGGCCAT CGTTCTGCTG TCCATCATGG GCATCACGTT GGAGGAGATC
    GAGATGAATG CAGCGCTGCC CATCAACCCC ACCATCATCC GAATCATGCG TGTCCTCCGT
    ATCGCCCGCG TCCTGAAGCT GCTCAAGATG GCCACGGGCA TGCGGGCCCT GCTGGACACG
    GTGGTCCAGG CGCTGCCCCA GGTAGGGAAC CTCGGCCTGC TTTTCATGCT CCTGTTTTTT
    ATCTATGCTG CCCTGGGAGT GGAGCTGTTC GGGAGGCTAG AATGCAGTGA GGACAACCCC
    TGCGAGGGCC TGAGCAGACA CGCCACCTTC TCTAACTTCG GCATGGCCTT CCTCACGCTG
    TTCCGCGTGT CCACCGGGGA CAACTGGAAC GGGATCATGA AGGACACGCT GCGCGAGTGC
    GCCCGCGAGG ACAAGCACTG CCTCACCTAC CTGCCGGCCA TCTCGCCCAT CTACTTCGTC
    ACCTTCGTGC TGGTGGCCCA GTTCGTGCTT GTCAACGTGG TGGTGGCCGT GCTCATGAAG
    CACCTGGAGG AGAGCAACAA GGAGGCCCGC GAGGACGCCG AGCTGGACGC AGAGCTTGAG
    ATGGAAATGG CGCAGGGGTC CCCCGCCCGT CCCAGGCCGG CGGCCCCTCA GAGCCCCGGC
    GCCTCGCCGG ACTCCCCCAA CGTGCTGGTC GTGCGCAAGG TGTCGGTGTC CAGGATGCTA
    TCCCTGCCCA ACGACAGCTA CATGTTCCGG CCCGTGGCGC CTGCGGCAGC CGCTCACCCC
    CACCCGCTAC AGGAGGTGGA GATGGAGACC TACGCGGGCG CGGCCGCCGG CCAGGTCACC
    GCTGCCCACT CGCCGCCTGT GGAGTCCTGC GCCTCCCTCC AGGTTCCGTC CGCCGTGTCC
    TCCCCGTCCA GGGGCGGCGA CACCCTCCGT GCCCTGCCCC CACGGGGTGC AGCCCGGTCG
    CCTAATGTCG GCCGACTTCT CTGCAGACAG GAGGCCGTCC ACACGGAGTC CCTGGAAGGG
    CATGTCGATG GCACTGAGGA CAGCAGCCCA TTCTGGGGAG AGCCTGGCGG GAAGACCCCC
    GTGAGACAGG CATCCCTGGG GCCCTCCTTG CGGTCCCCAT CCCGGTCCCC GCGACCCTCC
    AGCATCCGCA CCCGCAAGCA CACTTCTGGA CAGCACTGCA TCTCCAGCCG GCCTCCTGCC
    CCAGGCGGGG AGGAGTCCGA AGCCCCAGAC CCAGCTGACG AGGAGGTCAG CCACATCACC
    AGCTCTGCCC GCAGCCCTTT GGCCTCGCCC ACTGCCTGTG GGATCGTGGG CGGCGAGCCA
    GACGTGCACA GGCTCTACAG CGTGGACGCC CGGGGCTTCC TGGACCAGCC GGGCCGGGTG
    GATGAGCAGA GGCGGCCCTT GGGGGAGCCA GGAGTTGGGG ACAGCCGTCC AGAGGCCGGG
    GAGGCGAAGC CCCGGGTCCT GGAGGCCGAG CTGGCCCTAG GGGCGCGCAG GAAGAAGAAG
    ATGAGCCCCC CCTGCATTTC CATAGAGCCC CCCGCTGAGG ACGAGGGTGC GGCCAGGGCC
    CCTGCGGCAG AAGGCGGCAG CACCACCCTG CGGCGGCGAA CCCCATCCTG CGAGGCTGTG
    CCCCATAGGG ACCCCCTGGA GCCCGCAGAC AGCACAGGGC TGGACGCTGC TGCCAAGGGG
    GAGCGGCGGG GCCAGGGCCC CTGCCGTACA GAACACCTGA CTGTCCCGAA CTTCACCTTT
    GAGCCACTGG ACGTGGGGAG CCCCAGTGGG GACCTGTTCA CAGATGCGGG CCATGGGGCC
    ACCCCAGAAC CCAGACCCTC CTCCTCAGGC ACCGCGGCCC CTCCAGAACC CCGGGAGATG
    GAGTCCACAG TGCCCTCAGG AGACCCCCTA GAGGAGGGGC GGGGGGTGCA CCTCACTGTC
    CCGGAGAGCC CACCGAAGAG AGCGTCCCCC CCGGCTGTGC CAGTCCCGGG TGACGACGTG
    GATGAGCCCG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315785.1
    CDS1..7035
    Translation

    Target ORF information:

    RefSeq Version XM_015460299.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, T type, alpha 1H subunit (CACNA1H), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460299.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCGCTCT CAGCGGGAAC GGAGGGGCAC CAAGGCTCCA GGGCACACAG CCCGCATGTG 
    CTGCAGGCGG TCCAGCCCAT CATCCTGTCC AGCCCCCTGG GCGCCGGGGG TGGGGCTAGG
    GGCCTCGGCC TGGCACTGCT GGCCCACCAC TTGGCTGCCT TCCGGAGGAG CAGAGGGGGA
    ACTTGGCGGT GCACTGTGGC TTCTGTGGAG CCGAGGCGCC ACCTGGTGGC CACTCTTAGA
    ATGTGCGTCC TCATTGAGGT GGCCGTCGGC ACCTGCTCCA CGTGGTTCGA GCATGTCAGC
    ATGCTGGTCA TCATGCTCAA CTGCGTGACC CTGGGCATGT TCCGGCCCTG CGAGGACGTC
    GAGTGCCGCT CGGAGCGCTG TGGCATCCTC GAGGCCTTTG ACGACTTCAT CTTCGCCTTC
    TTCGCGGTGG AGATGGTCAT CAAGATGATC GCCCTGGGGC TGTTTGGGCA GAAGTGCTAC
    TTGGGTGACA CGTGGAACAG GCTGGACTTC TTCATCGTCA TGGCGGGTAT GATGGAGTAC
    TCCTTGGACG GACACAACGT GAGCCTCTCG GCCATCCGAA CCGTGCGCGT GCTGCGGCCC
    CTTCGTGCCA TCAACCGTGT GCCCAGCATG CGGATCCTGG TCACGCTGCT GCTGGACACA
    CTGCCCATGC TCGGCAACGT TCTCCTGCTC TGCTTCTTTG TCTTCTTCAT CTTCGGCATC
    GTGGGCGTCC AGCTTTGGGC CGGCCTGCTG CGCAACCGCT GCTTCCTGGA CAGCACCTTC
    GCCAGGAACA GCAACCTCAG CTTCCTGCGG CCGTACTACC AGCCGGAGGA GGGCGAAGAA
    AACCCGTTCA TCTGCTCCTC CCGCCGGGAC AACGGCATGC AGAAGTGCTC GCACATCCCC
    AGCCGCCGCG AGCTGCGCGT GGAGTGCACG CTGGGCTGGG AGGCCTACGG GCAGCCGCAG
    GCCGAGGGCG CAGGTGGCAC GGGTCACCAC ACCTGCATCA ACTGGAACCA GTACTACAAT
    GTGTGCCGCT CGGGCGACTC CAACCCGCAC AACGGGGCCA TCAGCTTCGA CAACATCGGC
    TACGCCTGGA TCGCCATCTT CCAGGTGATC ACGCTGGAGG GCTGGGTGGA CATCATGTAC
    TACGTCATGG ACGCCCACTC TTTCTACAAC TTCATCTACT TCATCTTGCT TATCATCGTG
    GGCTCCTTCT TCATGATCAA CCTGTGCCTG GTGGTCATCG CCACGCAGTT CTCAGAGACG
    AAGCAGCGGG AGAACCAGCT GATGCGGGAG CAGCGGGCCC GTTATCTGTC CAATGACAGC
    ACGCTAGCCA GCTTCTCTGA GCCGGGCAGC TGCTATGAGG AGCTTCTCCG GTACGTGGGC
    CACGTGTGCC GCAAGCTAAA GCGCCGTGGC CTGCGCCTCT ACGCCCGCTG GCAGAGCCGC
    TGGCGCAAGA AGGTGGAGCC CGGTGGCGCC ACGCATGGCC AGGGCTCAGG GCGGCGGCCG
    CGGCGGGCAG GCGGGCGCGC TGCCTCCATC CACCACCTCG TGTACCACCA CCACCACCAT
    CACCACCACC ACTACCACTT CAGCCACGGC AGCCCCCGCC GGCCAGGCCC CGAGCCAGGA
    GCCGGCGACA CTAGGCTGGT GCGGGCCGGA GCGCCAGCCT CACCCGGGCG CGGGCCCCCT
    GACGCCGAGT CGGTGCACAG TGTGTACCAC GCTGACTGCC ACGTGGAGGG GCCACAGGAG
    AGGGCGCGCG TGGCACACGC CGCAGCCACT GCCGCTGCTG GGCTCAAGCT GGCCACCGGG
    CTGGGAGCCA TGAACTACCC CACCATCCTG CCCTCGGCTG CGGGCAGTGG CAAAAGCGGC
    CTCGGGCCCA AGGGGAAGCG GCCTGGTGGC CCCCCGGGAG CCGGGGGGCA CAGCCCCCTG
    AGGCTGAGCA GCCCGGACCC CTGCGAGAAG ATCCAGCACC TGGTTGGGGA GCATGGACTG
    GGCCAGGCCC CCAGCCGCCT GTCAGGCCTG AGTGTGCCCT GCCCCCTGCC CAGCCCCCAG
    GCGGGCACGC TGACCTGTGA GCTGAGCAGC TGCCCGTACT GCACCAGTGC CCTGGAGGGC
    CCCGAGCTGG AGTTCAGCGA CTCAGACAGT GGAGACTCAG ACAGCAATGG GGTCTACGAG
    TTCACGCAGG ATGTGCGGCA CGGGGACCAC CGCGACCCCA TGCAGTCGCC CCCCGTGGCA
    GAGGCGCCAG GCGTGGGCGG CACACGGCGG AGGGGGCCGC AGCGGGCGGT GGCAGGCGAG
    CAGGGTGGGC TGGGCCACGT CTGGGCCTCC TTCAGTGGCA AGCTGCGCCG CATCGTGGAC
    AGCAAGTACT TCAACCGGGG CATCATGGTG GCCATCCTCA CTAACACGCT GAGCATGGGT
    GTCGAGTACC ACGAGCAGCC CGATGACCGA ACCTGCTCCC AGGTGGGGTT GAGCAGAGGC
    CGAGGTGGCT CCAGGCTGTC GTTCCTGCCT CTGGGAAGAG AGTGTCCCAG AATCAGGGCG
    GGAGTAGGCC ATCCTAACCC CGCCCACCCC ACCCCACCCC CGACCCCCAG CGTGTGGGAG
    ATCATCGGGC AGGCGGACGG CGGGCTGTCG GTGCTGCGCA CCTTCCGGCT GCTGCGGGTG
    CTCAAGCTGG TGCGCTTCAT GCCCGCACTG CGGCGCCAGC TGGTGGTGCT CATGAAGACC
    ATGGACAACG TGGCCACCTT CTGCATGCTG CTCATGCTTT TCATCTTCAT CTTCAGCATC
    CTTGGCATGC ATCTCTTTGG CTGCAAATTC AGTCTGAAAA CAGATACCGG AGACACAATC
    CCCGACCGGA AGAACTTTGA TTCCCTACTG TGGGCCATCG TCACCGTGTT CCAGATCCTC
    ACCCAGGAGG ACTGGAACGT CGTCCTGTAC AACGGCATGG CCTCCACGTC CTCCTGGGCC
    GCCCTGTACT TCGTGGCCCT CATGACCTTT GGCAACTACG TGCTCTTCAA CCTGCTGGTG
    GCCATCCTGG TGGAGGGTTT CCAGGCCGAG GGTGATGCCA CCAGGTCCGA CACAGATGAA
    GACAAGACAT CCACCCACTT GGAGGAGGAC TTGGACAAGT TCCGAGACCT CAGGGCCACA
    GAGATGAAGA TGTACTCGCT GGCAGTGACA CCCAACGGGC ACCTGGAGGG CCGGGGCAGC
    CTGCCCCCTC CCCTTATCAT GCGCACAGCG GCCACGCCCA TGCCCACCCC CAAGAGCTCC
    CCACACCTGG ACGAGGCCCC TGGCCCCCTG GACTCGCGCC GTGGCAGCAG CAGCTCCATG
    GACCCTCAGC TAGGAGACCA GAAATCTCTG TCCAGCCTCC GCAGCTCGCC CTGCGCCCCC
    TGGGGGCCCA ACAGCGCCTG GAGCAGCCGG CGCTCCAGCT GGAACAGCCT GGGCCGTGCA
    CCCAGCCTCA AGCGCAGGAG CCAGTGTGGG GAGCGCGAGT CGCTGCTGTC CGGTGAGGGC
    AGGGGCAGCG GCAGCACTGA CGAGGAGGCC GAGGATGGCC GGCCCGGGGC GGGGGCCTCG
    CCGGGTACGC GTGCCACGCC GCTGCGGCGC ACCGAGTCCC TGGACCACCG TAGCACGCTG
    GACCTGCGGC CCCCACGGCC AGCCACCCTG CTGCCCACCA AGGTCCACGA CTGCAACGGG
    CAGGTGCTGG CCCTGCCGAG CGAGTTCTTC CTGCGCATCG ACAGCCACAA GGAGGATCCG
    GCCGAGTTTG ACGACGATGT GGAGGACAGC TGCTGCTCAA GGCTGCAGAA GGTGCTGGAG
    CCGCATAAGC CGGAGTGCTG CCGGAGCCGC GAGCCCTGGG CCCTGTACCT CTTCTCCCCA
    CAGAACAGGT TCCGCATCTC CTGCCAAAAG ATCATTGCTC ACAAGATGTT CGATCATGTC
    GTCCTCGTCT TCATCTTCCT CAACTGCATC ACCATCGCCC TGGAGAGGCC CGACATCGAC
    CCGGCAGAGC GCGTCTTCCT CAGCGTCTCC AACTACATCT TCACAGCGAT CTTCGTGGCG
    GAGATGATGG TGAAGGTGGT GGCCCTGGGT CTCATCTCGG GTGAGCATGC CTACCTGCAG
    AGCAGCTGGA ACATACTGGA TGGGGTGCTG GTCCTGGTGT CCCTGGTGGA CATCATCGTG
    GCCATGGCCT CGGCTGGCGG CGCCAAGATC CTGGGCATCC TGCGTGTGCT GCGCCTGCTG
    CGGACCCTGC GGCCGCTAAG GGTCATCAGC CGTGCGCCGG GACTCAAGCT GGTGGTGGAG
    ACTCTGATAT CGTCCCTCAG GCCCATCGGG AACATCGTCC TCATCTGCTG CGCCTTCTTC
    ATCATCTTCG GCATCCTCGG GGTGCAGCTC TTCAAGGGGA AGTTTTACTA CTGCGAGGGC
    GCCGACACCA GGAACATTTC CACTAAGGCC GAGTGCAGGG CCGCGCACTA CCGCTGGGTG
    CGACGCAAGT ACAACTTCGA CAACCTGGGC CAGGCGCTGA TGTCGCTGTT CGTGCTCTCG
    TCCAAGGACG GCTGGGTGAA TATCATGTAC GACGGGCTGG ACGCCGTGGG CGTGGACCAG
    CAGCCCGTGC CCAACCACAA CCCCTGGATG CTGCTCTACT TCATCTCCTT CCTGCTCATC
    GTCAGCTTCT TCGTGCTCAA CATGTTCGTG GGCGTCGTGG TGGAGAACTT CCACAAGTGC
    CGGCAGCACC AGGAGGCCGA GGAGGCGCGG CGGCGCGAGG AAAAGCGGCA GCGTCGCCTG
    GAGAGGAAGC GCAGGAGCAC TTTCCCTAAC CCAGAGGCCC AGCGACGGCC CTACTATGCG
    GACTACTCGC CCACCCGCCG CTCCATCCAC ACTCTGTGCA CCAGCCACTA CCTGGACCTC
    TTCATCACCT TCATCATTGG CGTCAACGTC ATCACCATGT CCATGGAGCA CTACAACCAG
    CCCAAGGCTC TGGACGAGGC CCTCAAGTAC TGCAATTACG TGTTCACCAT CGTCTTTGTC
    GTCGAGGCCG TGCTGAAGCT GGTGGCCTTT GGGTTCCGGA GGTTCTTCAA GGACAGGTGG
    AACCAGCTGG ACCTGGCCAT CGTTCTGCTG TCCATCATGG GCATCACGTT GGAGGAGATC
    GAGATGAATG CAGCGCTGCC CATCAACCCC ACCATCATCC GAATCATGCG TGTCCTCCGT
    ATCGCCCGCG TCCTGAAGCT GCTCAAGATG GCCACGGGCA TGCGGGCCCT GCTGGACACG
    GTGGTCCAGG CGCTGCCCCA GGTAGGGAAC CTCGGCCTGC TTTTCATGCT CCTGTTTTTT
    ATCTATGCTG CCCTGGGAGT GGAGCTGTTC GGGAGGCTAG AATGCAGTGA GGACAACCCC
    TGCGAGGGCC TGAGCAGACA CGCCACCTTC TCTAACTTCG GCATGGCCTT CCTCACGCTG
    TTCCGCGTGT CCACCGGGGA CAACTGGAAC GGGATCATGA AGGACACGCT GCGCGAGTGC
    GCCCGCGAGG ACAAGCACTG CCTCACCTAC CTGCCGGCCA TCTCGCCCAT CTACTTCGTC
    ACCTTCGTGC TGGTGGCCCA GTTCGTGCTT GTCAACGTGG TGGTGGCCGT GCTCATGAAG
    CACCTGGAGG AGAGCAACAA GGAGGCCCGC GAGGACGCCG AGCTGGACGC AGAGCTTGAG
    ATGGAAATGG CGCAGGGGTC CCCCGCCCGT CCCAGGCCGG CGGCCCCTCA GAGCCCCGGC
    GCCTCGCCGG ACTCCCCCAA CGTGCTGGTC GTGCGCAAGG TGTCGGTGTC CAGGATGCTA
    TCCCTGCCCA ACGACAGCTA CATGTTCCGG CCCGTGGCGC CTGCGGCAGC CGCTCACCCC
    CACCCGCTAC AGGAGGTGGA GATGGAGACC TACGCGGGCG CGGCCGCCGG CCAGGTCACC
    GCTGCCCACT CGCCGCCTGT GGAGTCCTGC GCCTCCCTCC AGGTTCCGTC CGCCGTGTCC
    TCCCCGTCCA GGGGCGGCGA CACCCTCCGT GCCCTGCCCC CACGGGGTGC AGCCCGGTCG
    CCTAATGTCG GCCGACTTCT CTGCAGACAG GAGGCCGTCC ACACGGAGTC CCTGGAAGGG
    CATGTCGATG GCACTGAGGA CAGCAGCCCA TTCTGGGGAG AGCCTGGCGG GAAGACCCCC
    GTGAGACAGG CATCCCTGGG GCCCTCCTTG CGGTCCCCAT CCCGGTCCCC GCGACCCTCC
    AGCATCCGCA CCCGCAAGCA CACTTCTGGA CAGCACTGCA TCTCCAGCCG GCCTCCTGCC
    CCAGGCGGGG AGGAGTCCGA AGCCCCAGAC CCAGCTGACG AGGAGGTCAG CCACATCACC
    AGCTCTGCCC GCAGCCCTTT GGCCTCGCCC ACTGCCTGTG GGATCGTGGG CGGCGAGCCA
    GACGTGCACA GGCTCTACAG CGTGGACGCC CGGGGCTTCC TGGACCAGCC GGGCCGGGTG
    GATGAGCAGA GGCGGCCCTT GGGGGAGCCA GGAGTTGGGG ACAGCCGTCC AGAGGCCGGG
    GAGGCGAAGC CCCGGGTCCT GGAGGCCGAG CTGGCCCTAG GGGCGCGCAG GAAGAAGAAG
    ATGAGCCCCC CCTGCATTTC CATAGAGCCC CCCGCTGAGG ACGAGGGTGC GGCCAGGGCC
    CCTGCGGCAG AAGGCGGCAG CACCACCCTG CGGCGGCGAA CCCCATCCTG CGAGGCTGTG
    CCCCATAGGG ACCCCCTGGA GCCCGCAGAC AGCACAGGGC TGGACGCTGC TGCCAAGGGG
    GAGCGGCGGG GCCAGGGCCC CTGCCGTACA GAACACCTGA CTGTCCCGAA CTTCACCTTT
    GAGCCACTGG ACGTGGGGAG CCCCAGTGGG GACCTGTTCA CAGATGCGGG CCATGGGGCC
    ACCCCAGAAC CCAGACCCTC CTCCTCAGGC ACCGCGGCCC CTCCAGAACC CCGGGAGATG
    GAGTCCACAG TGCCCTCAGG AGACCCCCTA GAGGAGGGGC GGGGGGTGCA CCTCACTGTC
    CCGGAGAGCC CACCGAAGAG AGCGTCCCCC CCGGCTGTGC CAGTCCCGGG TGACGACGTG
    GATGAGCCCG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa95201
    Clone ID Related Accession (Same CDS sequence) XM_015469221.1
    Accession Version XM_015469221.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7041bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: voltage-dependent T-type calcium channel subunit alpha-1H isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646645.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGACCGAGG GCGTGCAGGC GGCCGACGAG GTCCGCGTGC CCNTGGGCGC GCCAGCCCCG 
    GGCCCGCGGC GGCGGCGGCG TCCCCGCGAC CTGGGGCCCG GGCCAGAGGC GGAGCGGGGG
    TCGGGGCCCA GTGCGTCGCC CCCGAGAGTC GGCGGCCGAG CGCGGGCCGA GCTTGGCGCC
    GACGAGGAGC AGCCCGTCCG TAACGGCGCT GGCGGCCACC GTCTTTCTTC TGTCTCGCGG
    CAGACCACGC GGCCGCGCAG CTGGTGTCTC CGGCTGGTCT GTAACCCGTG GTTCGAGCAT
    GTCAGCATGC TGGTCATCAT GCTCAACTGC GTGACCCTGG GCATGTTCCG GCCCTGCGAG
    GACGTCGAGT GCCGCTCGGA GCGCTGTGGC ATCCTCGAGG CCTTTGACGA CTTCATCTTC
    GCCTTCTTCG CGGTGGAGAT GGTCATCAAG ATGATCGCCC TGGGGCTGTT TGGGCAGAAG
    TGCTACTTGG GTGACACGTG GAACAGGCTG GACTTCTTCA TCGTCATGGC GGGTATGATG
    GAGTACTCCT TGGACGGACA CAACGTGAGC CTCTCGGCCA TCCGAACCGT GCGCGTGCTG
    CGGCCCCTTC GTGCCATCAA CCGTGTGCCC AGCATGCGGA TCCTGGTCAC GCTGCTGCTG
    GACACACTGC CCATGCTCGG CAACGTTCTC CTGCTCTGCT TCTTTGTCTT CTTCATCTTC
    GGCATCGTGG GCGTCCAGCT TTGGGCCGGC CTGCTGCGCA ACCGCTGCTT CCTGGACAGC
    ACCTTCGCCA GGAACAGCAA CCTCAGCTTC CTGCGGCCGT ACTACCAGCC GGAGGAGGGC
    GAAGAAAACC CGTTCATCTG CTCCTCCCGC CGGGACAACG GCATGCAGAA GTGCTCGCAC
    ATCCCCAGCC GCCGCGAGCT GCGCGTGGAG TGCACGCTGG GCTGGGAGGC CTACGGGCAG
    CCGCAGGCCG AGGGCGCAGG TGGCACGGGT CACCACACCT GCATCAACTG GAACCAGTAC
    TACAATGTGT GCCGCTCGGG CGACTCCAAC CCGCACAACG GGGCCATCAG CTTCGACAAC
    ATCGGCTACG CCTGGATCGC CATCTTCCAG GTGATCACGC TGGAGGGCTG GGTGGACATC
    ATGTACTACG TCATGGACGC CCACTCTTTC TACAACTTCA TCTACTTCAT CTTGCTTATC
    ATCGTGGGCT CCTTCTTCAT GATCAACCTG TGCCTGGTGG TCATCGCCAC GCAGTTCTCA
    GAGACGAAGC AGCGGGAGAA CCAGCTGATG CGGGAGCAGC GGGCCCGTTA TCTGTCCAAT
    GACAGCACGC TAGCCAGCTT CTCTGAGCCG GGCAGCTGCT ATGAGGAGCT TCTCCGGTAC
    GTGGGCCACG TGTGCCGCAA GCTAAAGCGC CGTGGCCTGC GCCTCTACGC CCGCTGGCAG
    AGCCGCTGGC GCAAGAAGGT GGAGCCCGGT GGCGCCACGC ATGGCCAGGG CTCAGGGCGG
    CGGCCGCGGC GGGCAGGCGG GCGCGCTGCC TCCATCCACC ACCTCGTGTA CCACCACCAC
    CACCATCACC ACCACCACTA CCACTTCAGC CACGGCAGCC CCCGCCGGCC AGGCCCCGAG
    CCAGGAGCCG GCGACACTAG GCTGGTGCGG GCCGGAGCGC CAGCCTCACC CGGGCGCGGG
    CCCCCTGACG CCGAGTCGGT GCACAGTGTG TACCACGCTG ACTGCCACGT GGAGGGGCCA
    CAGGAGAGGG CGCGCGTGGC ACACGCCGCA GCCACTGCCG CTGCTGGGCT CAAGCTGGCC
    ACCGGGCTGG GAGCCATGAA CTACCCCACC ATCCTGCCCT CGGCTGCGGG CAGTGGCAAA
    AGCGGCCTCG GGCCCAAGGG GAAGCGGCCT GGTGGCCCCC CGGGAGCCGG GGGGCACAGC
    CCCCTGAGGC TGAGCAGCCC GGACCCCTGC GAGAAGATCC AGCACCTGGT TGGGGAGCAT
    GGACTGGGCC AGGCCCCCAG CCGCCTGTCA GGCCTGAGTG TGCCCTGCCC CCTGCCCAGC
    CCCCAGGCGG GCACGCTGAC CTGTGAGCTG AGCAGCTGCC CGTACTGCAC CAGTGCCCTG
    GAGGGCCCCG AGCTGGAGTT CAGCGACTCA GACAGTGGAG ACTCAGACAG CAATGGGGTC
    TACGAGTTCA CGCAGGATGT GCGGCACGGG GACCACCGCG ACCCCATGCA GTCGCCCCCC
    GTGGCAGAGG CGCCAGGCGT GGGCGGCACA CGGCGGAGGG GGCCGCAGCG GGCGGTGGCA
    GGCGAGCAGG GTGGGCTGGG CCACGTCTGG GCCTCCTTCA GTGGCAAGCT GCGCCGCATC
    GTGGACAGCA AGTACTTCAA CCGGGGCATC ATGGTGGCCA TCCTCACTAA CACGCTGAGC
    ATGGGTGTCG AGTACCACGA GCAGCCCGAT GAGCTGACCA ACGCCCTGGA GATCAGCAAC
    ATTGTGTTCA CAACATCGTT TGCTCTGGAG ATGCTGTTGA AGCTGCTGCC TGCGGCCACT
    GGGGCTAACA TCCGGAACCC TTACAACATC TTTGACGGCA TCATCGTAGT CATCAGCGTG
    TGGGAGATCA TCGGGCAGGC GGACGGCGGG CTGTCGGTGC TGCGCACCTT CCGGCTGCTG
    CGGGTGCTCA AGCTGGTGCG CTTCATGCCC GCACTGCGGC GCCAGCTGGT GGTGCTCATG
    AAGACCATGG ACAACGTGGC CACCTTCTGC ATGCTGCTCA TGCTTTTCAT CTTCATCTTC
    AGCATCCTTG GCATGCATCT CTTTGGCTGC AAATTCAGTC TGAAAACAGA TACCGGAGAC
    ACAATCCCCG ACCGGAAGAA CTTTGATTCC CTACTGTGGG CCATCGTCAC CGTGTTCCAG
    ATCCTCACCC AGGAGGACTG GAACGTCGTC CTGTACAACG GCATGGCCTC CACGTCCTCC
    TGGGCCGCCC TGTACTTCGT GGCCCTCATG ACCTTTGGCA ACTACGTGCT CTTCAACCTG
    CTGGTGGCCA TCCTGGTGGA GGGTTTCCAG GCCGAGGGTG ATGCCACCAG GTCCGACACA
    GATGAAGACA AGACATCCAC CCACTTGGAG GAGGACTTGG ACAAGTTCCG AGACCTCAGG
    GCCACAGAGA TGAAGATGTA CTCGCTGGCA GTGACACCCA ACGGGCACCT GGAGGGCCGG
    GGCAGCCTGC CCCCTCCCCT TATCATGCGC ACAGCGGCCA CGCCCATGCC CACCCCCAAG
    AGCTCCCCAC ACCTGGACGA GGCCCCTGGC CCCCTGGACT CGCGCCGTGG CAGCAGCAGC
    TCCATGGACC CTCAGCTAGG AGACCAGAAA TCTCTGTCCA GCCTCCGCAG CTCGCCCTGC
    GCCCCCTGGG GGCCCAACAG CGCCTGGAGC AGCCGGCGCT CCAGCTGGAA CAGCCTGGGC
    CGTGCACCCA GCCTCAAGCG CAGGAGCCAG TGTGGGGAGC GCGAGTCGCT GCTGTCCGGT
    GAGGGCAGGG GCAGCGGCAG CACTGACGAG GAGGCCGAGG ATGGCCGGCC CGGGGCGGGG
    GCCTCGCCGG GTACGCGTGC CACGCCGCTG CGGCGCACCG AGTCCCTGGA CCACCGTAGC
    ACGCTGGACC TGCGGCCCCC ACGGCCAGCC ACCCTGCTGC CCACCAAGGT CCACGACTGC
    AACGGGCAGG TGCTGGCCCT GCCGAGCGAG TTCTTCCTGC GCATCGACAG CCACAAGGAG
    GATCCGGCCG AGTTTGACGA CGATGTGGAG GACAGCTGCT GCTCAAGGCT GCAGAAGGTG
    CTGGAGCCGC ATAAGCCGGA GTGCTGCCGG AGCCGCGAGC CCTGGGCCCT GTACCTCTTC
    TCCCCACAGA ACAGGTTCCG CATCTCCTGC CAAAAGATCA TTGCTCACAA GATGTTCGAT
    CATGTCGTCC TCGTCTTCAT CTTCCTCAAC TGCATCACCA TCGCCCTGGA GAGGCCCGAC
    ATCGACCCGG CAGAGCGCGT CTTCCTCAGC GTCTCCAACT ACATCTTCAC AGCGATCTTC
    GTGGCGGAGA TGATGGTGAA GGTGGTGGCC CTGGGTCTCA TCTCGGGTGA GCATGCCTAC
    CTGCAGAGCA GCTGGAACAT ACTGGATGGG GTGCTGGTCC TGGTGTCCCT GGTGGACATC
    ATCGTGGCCA TGGCCTCGGC TGGCGGCGCC AAGATCCTGG GCATCCTGCG TGTGCTGCGC
    CTGCTGCGGA CCCTGCGGCC GCTAAGGGTC ATCAGCCGTG CGCCGGGACT CAAGCTGGTG
    GTGGAGACTC TGATATCGTC CCTCAGGCCC ATCGGGAACA TCGTCCTCAT CTGCTGCGCC
    TTCTTCATCA TCTTCGGCAT CCTCGGGGTG CAGCTCTTCA AGGGGAAGTT TTACTACTGC
    GAGGGCGCCG ACACCAGGAA CATTTCCACT AAGGCCGAGT GCAGGGCCGC GCACTACCGC
    TGGGTGCGAC GCAAGTACAA CTTCGACAAC CTGGGCCAGG CGCTGATGTC GCTGTTCGTG
    CTCTCGTCCA AGGACGGCTG GGTGAATATC ATGTACGACG GGCTGGACGC CGTGGGCGTG
    GACCAGCAGC CCGTGCCCAA CCACAACCCC TGGATGCTGC TCTACTTCAT CTCCTTCCTG
    CTCATCGTCA GCTTCTTCGT GCTCAACATG TTCGTGGGCG TCGTGGTGGA GAACTTCCAC
    AAGTGCCGGC AGCACCAGGA GGCCGAGGAG GCGCGGCGGC GCGAGGAAAA GCGGCAGCGT
    CGCCTGGAGA GGAAGCGCAG GAGCACTTTC CCTAACCCAG AGGCCCAGCG ACGGCCCTAC
    TATGCGGACT ACTCGCCCAC CCGCCGCTCC ATCCACACTC TGTGCACCAG CCACTACCTG
    GACCTCTTCA TCACCTTCAT CATTGGCGTC AACGTCATCA CCATGTCCAT GGAGCACTAC
    AACCAGCCCA AGGCTCTGGA CGAGGCCCTC AAGTACTGCA ATTACGTGTT CACCATCGTC
    TTTGTCGTCG AGGCCGTGCT GAAGCTGGTG GCCTTTGGGT TCCGGAGGTT CTTCAAGGAC
    AGGTGGAACC AGCTGGACCT GGCCATCGTT CTGCTGTCCA TCATGGGCAT CACGTTGGAG
    GAGATCGAGA TGAATGCAGC GCTGCCCATC AACCCCACCA TCATCCGAAT CATGCGTGTC
    CTCCGTATCG CCCGCGTCCT GAAGCTGCTC AAGATGGCCA CGGGCATGCG GGCCCTGCTG
    GACACGGTGG TCCAGGCGCT GCCCCAGGTA GGGAACCTCG GCCTGCTTTT CATGCTCCTG
    TTTTTTATCT ATGCTGCCCT GGGAGTGGAG CTGTTCGGGA GGCTAGAATG CAGTGAGGAC
    AACCCCTGCG AGGGCCTGAG CAGACACGCC ACCTTCTCTA ACTTCGGCAT GGCCTTCCTC
    ACGCTGTTCC GCGTGTCCAC CGGGGACAAC TGGAACGGGA TCATGAAGGA CACGCTGCGC
    GAGTGCGCCC GCGAGGACAA GCACTGCCTC ACCTACCTGC CGGCCATCTC GCCCATCTAC
    TTCGTCACCT TCGTGCTGGT GGCCCAGTTC GTGCTTGTCA ACGTGGTGGT GGCCGTGCTC
    ATGAAGCACC TGGAGGAGAG CAACAAGGAG GCCCGCGAGG ACGCCGAGCT GGACGCAGAG
    CTTGAGATGG AAATGGCGCA GGGGTCCCCC GCCCGTCCCA GGCCGGCGGC CCCTCAGAGC
    CCCGGCGCCT CGCCGGACTC CCCCAACGTG CTGGTCGTGC GCAAGGTGTC GGTGTCCAGG
    ATGCTATCCC TGCCCAACGA CAGCTACATG TTCCGGCCCG TGGCGCCTGC GGCAGCCGCT
    CACCCCCACC CGCTACAGGA GGTGGAGATG GAGACCTACG CGGGCGCGGC CGCCGGCCAG
    GTCACCGCTG CCCACTCGCC GCCTGTGGAG TCCTGCGCCT CCCTCCAGGT TCCGTCCGCC
    GTGTCCTCCC CGTCCAGGGG CGGCGACACC CTCCGTGCCC TGCCCCCACG GGGTGCAGCC
    CGGTCGCCTA ATGTCGGCCG ACTTCTCTGC AGACAGGAGG CCGTCCACAC GGAGTCCCTG
    GAAGGGCATG TCGATGGCAC TGAGGACAGC AGCCCATTCT GGGGAGAGCC TGGCGGGAAG
    ACCCCCGTGA GACAGGCATC CCTGGGGCCC TCCTTGCGGT CCCCATCCCG GTCCCCGCGA
    CCCTCCAGCA TCCGCACCCG CAAGCACACT TCTGGACAGC ACTGCATCTC CAGCCGGCCT
    CCTGCCCCAG GCGGGGAGGA GTCCGAAGCC CCAGACCCAG CTGACGAGGA GGTCAGCCAC
    ATCACCAGCT CTGCCCGCAG CCCTTTGGCC TCGCCCACTG CCTGTGGGAT CGTGGGCGGC
    GAGCCAGACG TGCACAGGCT CTACAGCGTG GACGCCCGGG GCTTCCTGGA CCAGCCGGGC
    CGGGTGGATG AGCAGAGGCG GCCCTTGGGG GAGCCAGGAG TTGGGGACAG CCGTCCAGAG
    GCCGGGGAGG CGAAGCCCCG GGTCCTGGAG GCCGAGCTGG CCCTAGGGGC GCGCAGGAAG
    AAGAAGATGA GCCCCCCCTG CATTTCCATA GAGCCCCCCG CTGAGGACGA GGGTGCGGCC
    AGGGCCCCTG CGGCAGAAGG CGGCAGCACC ACCCTGCGGC GGCGAACCCC ATCCTGCGAG
    GCTGTGCCCC ATAGGGACCC CCTGGAGCCC GCAGACAGCA CAGGGCTGGA CGCTGCTGCC
    AAGGGGGAGC GGCGGGGCCA GGGCCCCTGC CGTACAGAAC ACCTGACTGT CCCGAACTTC
    ACCTTTGAGC CACTGGACGT GGGGAGCCCC AGTGGGGACC TGTTCACAGA TGCGGGCCAT
    GGGGCCACCC CAGAACCCAG ACCCTCCTCC TCAGGCACCG CGGCCCCTCC AGAACCCCGG
    GAGATGGAGT CCACAGTGCC CTCAGGAGAC CCCCTAGAGG AGGGGCGGGG GGTGCACCTC
    ACTGTCCCGG AGAGCCCACC GAAGAGAGCG TCCCCCCCGG CTGTGCCAGT CCCGGGTGAC
    GACGTGGATG AGCCCGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324707.1
    CDS103..7143
    Translation

    Target ORF information:

    RefSeq Version XM_015469221.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, T type, alpha 1H subunit (CACNA1H), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469221.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGACCGAGG GCGTGCAGGC GGCCGACGAG GTCCGCGTGC CCNTGGGCGC GCCAGCCCCG 
    GGCCCGCGGC GGCGGCGGCG TCCCCGCGAC CTGGGGCCCG GGCCAGAGGC GGAGCGGGGG
    TCGGGGCCCA GTGCGTCGCC CCCGAGAGTC GGCGGCCGAG CGCGGGCCGA GCTTGGCGCC
    GACGAGGAGC AGCCCGTCCG TAACGGCGCT GGCGGCCACC GTCTTTCTTC TGTCTCGCGG
    CAGACCACGC GGCCGCGCAG CTGGTGTCTC CGGCTGGTCT GTAACCCGTG GTTCGAGCAT
    GTCAGCATGC TGGTCATCAT GCTCAACTGC GTGACCCTGG GCATGTTCCG GCCCTGCGAG
    GACGTCGAGT GCCGCTCGGA GCGCTGTGGC ATCCTCGAGG CCTTTGACGA CTTCATCTTC
    GCCTTCTTCG CGGTGGAGAT GGTCATCAAG ATGATCGCCC TGGGGCTGTT TGGGCAGAAG
    TGCTACTTGG GTGACACGTG GAACAGGCTG GACTTCTTCA TCGTCATGGC GGGTATGATG
    GAGTACTCCT TGGACGGACA CAACGTGAGC CTCTCGGCCA TCCGAACCGT GCGCGTGCTG
    CGGCCCCTTC GTGCCATCAA CCGTGTGCCC AGCATGCGGA TCCTGGTCAC GCTGCTGCTG
    GACACACTGC CCATGCTCGG CAACGTTCTC CTGCTCTGCT TCTTTGTCTT CTTCATCTTC
    GGCATCGTGG GCGTCCAGCT TTGGGCCGGC CTGCTGCGCA ACCGCTGCTT CCTGGACAGC
    ACCTTCGCCA GGAACAGCAA CCTCAGCTTC CTGCGGCCGT ACTACCAGCC GGAGGAGGGC
    GAAGAAAACC CGTTCATCTG CTCCTCCCGC CGGGACAACG GCATGCAGAA GTGCTCGCAC
    ATCCCCAGCC GCCGCGAGCT GCGCGTGGAG TGCACGCTGG GCTGGGAGGC CTACGGGCAG
    CCGCAGGCCG AGGGCGCAGG TGGCACGGGT CACCACACCT GCATCAACTG GAACCAGTAC
    TACAATGTGT GCCGCTCGGG CGACTCCAAC CCGCACAACG GGGCCATCAG CTTCGACAAC
    ATCGGCTACG CCTGGATCGC CATCTTCCAG GTGATCACGC TGGAGGGCTG GGTGGACATC
    ATGTACTACG TCATGGACGC CCACTCTTTC TACAACTTCA TCTACTTCAT CTTGCTTATC
    ATCGTGGGCT CCTTCTTCAT GATCAACCTG TGCCTGGTGG TCATCGCCAC GCAGTTCTCA
    GAGACGAAGC AGCGGGAGAA CCAGCTGATG CGGGAGCAGC GGGCCCGTTA TCTGTCCAAT
    GACAGCACGC TAGCCAGCTT CTCTGAGCCG GGCAGCTGCT ATGAGGAGCT TCTCCGGTAC
    GTGGGCCACG TGTGCCGCAA GCTAAAGCGC CGTGGCCTGC GCCTCTACGC CCGCTGGCAG
    AGCCGCTGGC GCAAGAAGGT GGAGCCCGGT GGCGCCACGC ATGGCCAGGG CTCAGGGCGG
    CGGCCGCGGC GGGCAGGCGG GCGCGCTGCC TCCATCCACC ACCTCGTGTA CCACCACCAC
    CACCATCACC ACCACCACTA CCACTTCAGC CACGGCAGCC CCCGCCGGCC AGGCCCCGAG
    CCAGGAGCCG GCGACACTAG GCTGGTGCGG GCCGGAGCGC CAGCCTCACC CGGGCGCGGG
    CCCCCTGACG CCGAGTCGGT GCACAGTGTG TACCACGCTG ACTGCCACGT GGAGGGGCCA
    CAGGAGAGGG CGCGCGTGGC ACACGCCGCA GCCACTGCCG CTGCTGGGCT CAAGCTGGCC
    ACCGGGCTGG GAGCCATGAA CTACCCCACC ATCCTGCCCT CGGCTGCGGG CAGTGGCAAA
    AGCGGCCTCG GGCCCAAGGG GAAGCGGCCT GGTGGCCCCC CGGGAGCCGG GGGGCACAGC
    CCCCTGAGGC TGAGCAGCCC GGACCCCTGC GAGAAGATCC AGCACCTGGT TGGGGAGCAT
    GGACTGGGCC AGGCCCCCAG CCGCCTGTCA GGCCTGAGTG TGCCCTGCCC CCTGCCCAGC
    CCCCAGGCGG GCACGCTGAC CTGTGAGCTG AGCAGCTGCC CGTACTGCAC CAGTGCCCTG
    GAGGGCCCCG AGCTGGAGTT CAGCGACTCA GACAGTGGAG ACTCAGACAG CAATGGGGTC
    TACGAGTTCA CGCAGGATGT GCGGCACGGG GACCACCGCG ACCCCATGCA GTCGCCCCCC
    GTGGCAGAGG CGCCAGGCGT GGGCGGCACA CGGCGGAGGG GGCCGCAGCG GGCGGTGGCA
    GGCGAGCAGG GTGGGCTGGG CCACGTCTGG GCCTCCTTCA GTGGCAAGCT GCGCCGCATC
    GTGGACAGCA AGTACTTCAA CCGGGGCATC ATGGTGGCCA TCCTCACTAA CACGCTGAGC
    ATGGGTGTCG AGTACCACGA GCAGCCCGAT GAGCTGACCA ACGCCCTGGA GATCAGCAAC
    ATTGTGTTCA CAACATCGTT TGCTCTGGAG ATGCTGTTGA AGCTGCTGCC TGCGGCCACT
    GGGGCTAACA TCCGGAACCC TTACAACATC TTTGACGGCA TCATCGTAGT CATCAGCGTG
    TGGGAGATCA TCGGGCAGGC GGACGGCGGG CTGTCGGTGC TGCGCACCTT CCGGCTGCTG
    CGGGTGCTCA AGCTGGTGCG CTTCATGCCC GCACTGCGGC GCCAGCTGGT GGTGCTCATG
    AAGACCATGG ACAACGTGGC CACCTTCTGC ATGCTGCTCA TGCTTTTCAT CTTCATCTTC
    AGCATCCTTG GCATGCATCT CTTTGGCTGC AAATTCAGTC TGAAAACAGA TACCGGAGAC
    ACAATCCCCG ACCGGAAGAA CTTTGATTCC CTACTGTGGG CCATCGTCAC CGTGTTCCAG
    ATCCTCACCC AGGAGGACTG GAACGTCGTC CTGTACAACG GCATGGCCTC CACGTCCTCC
    TGGGCCGCCC TGTACTTCGT GGCCCTCATG ACCTTTGGCA ACTACGTGCT CTTCAACCTG
    CTGGTGGCCA TCCTGGTGGA GGGTTTCCAG GCCGAGGGTG ATGCCACCAG GTCCGACACA
    GATGAAGACA AGACATCCAC CCACTTGGAG GAGGACTTGG ACAAGTTCCG AGACCTCAGG
    GCCACAGAGA TGAAGATGTA CTCGCTGGCA GTGACACCCA ACGGGCACCT GGAGGGCCGG
    GGCAGCCTGC CCCCTCCCCT TATCATGCGC ACAGCGGCCA CGCCCATGCC CACCCCCAAG
    AGCTCCCCAC ACCTGGACGA GGCCCCTGGC CCCCTGGACT CGCGCCGTGG CAGCAGCAGC
    TCCATGGACC CTCAGCTAGG AGACCAGAAA TCTCTGTCCA GCCTCCGCAG CTCGCCCTGC
    GCCCCCTGGG GGCCCAACAG CGCCTGGAGC AGCCGGCGCT CCAGCTGGAA CAGCCTGGGC
    CGTGCACCCA GCCTCAAGCG CAGGAGCCAG TGTGGGGAGC GCGAGTCGCT GCTGTCCGGT
    GAGGGCAGGG GCAGCGGCAG CACTGACGAG GAGGCCGAGG ATGGCCGGCC CGGGGCGGGG
    GCCTCGCCGG GTACGCGTGC CACGCCGCTG CGGCGCACCG AGTCCCTGGA CCACCGTAGC
    ACGCTGGACC TGCGGCCCCC ACGGCCAGCC ACCCTGCTGC CCACCAAGGT CCACGACTGC
    AACGGGCAGG TGCTGGCCCT GCCGAGCGAG TTCTTCCTGC GCATCGACAG CCACAAGGAG
    GATCCGGCCG AGTTTGACGA CGATGTGGAG GACAGCTGCT GCTCAAGGCT GCAGAAGGTG
    CTGGAGCCGC ATAAGCCGGA GTGCTGCCGG AGCCGCGAGC CCTGGGCCCT GTACCTCTTC
    TCCCCACAGA ACAGGTTCCG CATCTCCTGC CAAAAGATCA TTGCTCACAA GATGTTCGAT
    CATGTCGTCC TCGTCTTCAT CTTCCTCAAC TGCATCACCA TCGCCCTGGA GAGGCCCGAC
    ATCGACCCGG CAGAGCGCGT CTTCCTCAGC GTCTCCAACT ACATCTTCAC AGCGATCTTC
    GTGGCGGAGA TGATGGTGAA GGTGGTGGCC CTGGGTCTCA TCTCGGGTGA GCATGCCTAC
    CTGCAGAGCA GCTGGAACAT ACTGGATGGG GTGCTGGTCC TGGTGTCCCT GGTGGACATC
    ATCGTGGCCA TGGCCTCGGC TGGCGGCGCC AAGATCCTGG GCATCCTGCG TGTGCTGCGC
    CTGCTGCGGA CCCTGCGGCC GCTAAGGGTC ATCAGCCGTG CGCCGGGACT CAAGCTGGTG
    GTGGAGACTC TGATATCGTC CCTCAGGCCC ATCGGGAACA TCGTCCTCAT CTGCTGCGCC
    TTCTTCATCA TCTTCGGCAT CCTCGGGGTG CAGCTCTTCA AGGGGAAGTT TTACTACTGC
    GAGGGCGCCG ACACCAGGAA CATTTCCACT AAGGCCGAGT GCAGGGCCGC GCACTACCGC
    TGGGTGCGAC GCAAGTACAA CTTCGACAAC CTGGGCCAGG CGCTGATGTC GCTGTTCGTG
    CTCTCGTCCA AGGACGGCTG GGTGAATATC ATGTACGACG GGCTGGACGC CGTGGGCGTG
    GACCAGCAGC CCGTGCCCAA CCACAACCCC TGGATGCTGC TCTACTTCAT CTCCTTCCTG
    CTCATCGTCA GCTTCTTCGT GCTCAACATG TTCGTGGGCG TCGTGGTGGA GAACTTCCAC
    AAGTGCCGGC AGCACCAGGA GGCCGAGGAG GCGCGGCGGC GCGAGGAAAA GCGGCAGCGT
    CGCCTGGAGA GGAAGCGCAG GAGCACTTTC CCTAACCCAG AGGCCCAGCG ACGGCCCTAC
    TATGCGGACT ACTCGCCCAC CCGCCGCTCC ATCCACACTC TGTGCACCAG CCACTACCTG
    GACCTCTTCA TCACCTTCAT CATTGGCGTC AACGTCATCA CCATGTCCAT GGAGCACTAC
    AACCAGCCCA AGGCTCTGGA CGAGGCCCTC AAGTACTGCA ATTACGTGTT CACCATCGTC
    TTTGTCGTCG AGGCCGTGCT GAAGCTGGTG GCCTTTGGGT TCCGGAGGTT CTTCAAGGAC
    AGGTGGAACC AGCTGGACCT GGCCATCGTT CTGCTGTCCA TCATGGGCAT CACGTTGGAG
    GAGATCGAGA TGAATGCAGC GCTGCCCATC AACCCCACCA TCATCCGAAT CATGCGTGTC
    CTCCGTATCG CCCGCGTCCT GAAGCTGCTC AAGATGGCCA CGGGCATGCG GGCCCTGCTG
    GACACGGTGG TCCAGGCGCT GCCCCAGGTA GGGAACCTCG GCCTGCTTTT CATGCTCCTG
    TTTTTTATCT ATGCTGCCCT GGGAGTGGAG CTGTTCGGGA GGCTAGAATG CAGTGAGGAC
    AACCCCTGCG AGGGCCTGAG CAGACACGCC ACCTTCTCTA ACTTCGGCAT GGCCTTCCTC
    ACGCTGTTCC GCGTGTCCAC CGGGGACAAC TGGAACGGGA TCATGAAGGA CACGCTGCGC
    GAGTGCGCCC GCGAGGACAA GCACTGCCTC ACCTACCTGC CGGCCATCTC GCCCATCTAC
    TTCGTCACCT TCGTGCTGGT GGCCCAGTTC GTGCTTGTCA ACGTGGTGGT GGCCGTGCTC
    ATGAAGCACC TGGAGGAGAG CAACAAGGAG GCCCGCGAGG ACGCCGAGCT GGACGCAGAG
    CTTGAGATGG AAATGGCGCA GGGGTCCCCC GCCCGTCCCA GGCCGGCGGC CCCTCAGAGC
    CCCGGCGCCT CGCCGGACTC CCCCAACGTG CTGGTCGTGC GCAAGGTGTC GGTGTCCAGG
    ATGCTATCCC TGCCCAACGA CAGCTACATG TTCCGGCCCG TGGCGCCTGC GGCAGCCGCT
    CACCCCCACC CGCTACAGGA GGTGGAGATG GAGACCTACG CGGGCGCGGC CGCCGGCCAG
    GTCACCGCTG CCCACTCGCC GCCTGTGGAG TCCTGCGCCT CCCTCCAGGT TCCGTCCGCC
    GTGTCCTCCC CGTCCAGGGG CGGCGACACC CTCCGTGCCC TGCCCCCACG GGGTGCAGCC
    CGGTCGCCTA ATGTCGGCCG ACTTCTCTGC AGACAGGAGG CCGTCCACAC GGAGTCCCTG
    GAAGGGCATG TCGATGGCAC TGAGGACAGC AGCCCATTCT GGGGAGAGCC TGGCGGGAAG
    ACCCCCGTGA GACAGGCATC CCTGGGGCCC TCCTTGCGGT CCCCATCCCG GTCCCCGCGA
    CCCTCCAGCA TCCGCACCCG CAAGCACACT TCTGGACAGC ACTGCATCTC CAGCCGGCCT
    CCTGCCCCAG GCGGGGAGGA GTCCGAAGCC CCAGACCCAG CTGACGAGGA GGTCAGCCAC
    ATCACCAGCT CTGCCCGCAG CCCTTTGGCC TCGCCCACTG CCTGTGGGAT CGTGGGCGGC
    GAGCCAGACG TGCACAGGCT CTACAGCGTG GACGCCCGGG GCTTCCTGGA CCAGCCGGGC
    CGGGTGGATG AGCAGAGGCG GCCCTTGGGG GAGCCAGGAG TTGGGGACAG CCGTCCAGAG
    GCCGGGGAGG CGAAGCCCCG GGTCCTGGAG GCCGAGCTGG CCCTAGGGGC GCGCAGGAAG
    AAGAAGATGA GCCCCCCCTG CATTTCCATA GAGCCCCCCG CTGAGGACGA GGGTGCGGCC
    AGGGCCCCTG CGGCAGAAGG CGGCAGCACC ACCCTGCGGC GGCGAACCCC ATCCTGCGAG
    GCTGTGCCCC ATAGGGACCC CCTGGAGCCC GCAGACAGCA CAGGGCTGGA CGCTGCTGCC
    AAGGGGGAGC GGCGGGGCCA GGGCCCCTGC CGTACAGAAC ACCTGACTGT CCCGAACTTC
    ACCTTTGAGC CACTGGACGT GGGGAGCCCC AGTGGGGACC TGTTCACAGA TGCGGGCCAT
    GGGGCCACCC CAGAACCCAG ACCCTCCTCC TCAGGCACCG CGGCCCCTCC AGAACCCCGG
    GAGATGGAGT CCACAGTGCC CTCAGGAGAC CCCCTAGAGG AGGGGCGGGG GGTGCACCTC
    ACTGTCCCGG AGAGCCCACC GAAGAGAGCG TCCCCCCCGG CTGTGCCAGT CCCGGGTGAC
    GACGTGGATG AGCCCGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.