tmem131 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tmem131
Entrez Gene ID	557719
Full Name	transmembrane protein 131
General protein information	Preferred Names transmembrane protein 131 Names transmembrane protein 131 zmp:0000000659
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001923305.5	XP_001923340.1	transmembrane protein 131 isoform X2
XM_021477146.1	XP_021332821.1	transmembrane protein 131 isoform X1
XM_021477147.1	XP_021332822.1	transmembrane protein 131 isoform X2
XM_001923305.6	XP_001923340.1	transmembrane protein 131 isoform X2
XM_005165961.3	XP_005166018.1	transmembrane protein 131 isoform X1
XM_005165961.4	XP_005166018.1	transmembrane protein 131 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TMEM131	XP_515638.2
	TMEM131	XP_002808093.1
Danio rerio (zebrafish)	tmem131	XP_005166018.1
Xenopus tropicalis (tropical clawed frog)	tmem131	NP_001135552.1
Bos taurus (cattle)	TMEM131	XP_002691187.1
Homo sapiens (human)	TMEM131	NP_056163.1
Canis lupus familiaris (dog)	TMEM131	XP_531794.3
Rattus norvegicus (Norway rat)	Tmem131	XP_237056.4
Gallus gallus (chicken)	TMEM131	NP_001012712.2
Mus musculus (house mouse)	Tmem131	NP_061360.2

Gene Ontology

The following tmem131 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tmem131 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa11897	XM_005165961.4 Latest version!	Danio rerio transmembrane protein 131 (tmem131), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa11897	XM_021477146.1 Latest version!	Danio rerio transmembrane protein 131 (tmem131), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa11898	XM_021477147.1 Latest version!	Danio rerio transmembrane protein 131 (tmem131), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa11898	XM_001923305.6 Latest version!	Danio rerio transmembrane protein 131 (tmem131), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa11898	XM_001923305.5	Danio rerio transmembrane protein 131 (tmem131), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa11897	XM_005165961.3	Danio rerio transmembrane protein 131 (tmem131), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tmem131 ( XM_021477146.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332821.1 Danio rerio transmembrane protein 131 (tmem131), transcript variant X1, mRNA.

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CloneID	ODa11897
Clone ID Related Accession (Same CDS sequence)	XM_021477146.1 , XM_005165961.3 , XM_005165961.4
Accession Version	XM_021477146.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transmembrane protein 131 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCA AGGACACAAC ACAGAGTTCC CTACATCCGA CACGAACAAA ATCAGCATGT 
GTTGGACTTC TGCGCCTTTT ATTTATTGCA TTTATTCACG TGGCACAGGC GAACAAACAA 
GCTTTCATAC AGTCAGACAA CATCCTGGAG GTACTACACT TTGGGGATGG AAGCCTCTTG 
CAGACAGAGT CTGATATTGA CCTTAGCTCA TATCGTCAAA AAAGTGCATC CTCTCAGCGG 
GGGAACAGTA GGCCCATACG CTTTGAGCCT CCAATGCTGG ACTTTCATGA ACAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTTTACTTA CACAATCCAA GTTCAGAAGA AATAAGTTTA 
ATATCAATAT CAGCAACAAC ATCACATTTT CATGCATCCT TTTTTCAGAA TAGGATAATT 
CCACCTGGAG GCAACACCTC ATTTGATGTG GTGTTTCTAG CTAGAGTAGT AGGGAATGTA 
GAAAATACCT TATTTATTAA TACATCGCAT CATGGAGTGT TTACATACCA GGTGTTTGGT 
GTGGGCGTCC CCAATCCCTA CAGACTCAGG CCCTTTGTAG GGGCAAGAGT TCCAGTAAAT 
AGCAGCTTCT CCCCACTCAT AAGCATCTTC AACCCACACA GTGAACCACT GCAGGTTGTA 
GAGATGTACA CCAGTGGAGG AGACCTACAT TTAGAGCTGC CTACTGGACA GCAGGGTGGC 
ACCAAAAAAT TATGGGAGAT CGCTCCTTTT GAGACGAAGG GTGTGATGAG AGCCAGCTTC 
TCCTCTCGAG ACGCTGACAA CCACACAGCT TTCATCAGAA TAAAAACAAA TGCGTCCAAC 
GAGGACGAGT TTATCATCCT CCCTGTGGAG GTTGAAGTCA CCACAGCACC TGGTATATAC 
TCCTCCACAG AGATGCTGGA CTTTGGTACA CTTCGATCAC AAGATCGACC AAAACAACTG 
AATTTGCATC TGTTAAATTC AGGAGCAAAA GATGTGTCGA TCATGAGTGT TAGGCCAACT 
CCATCGAACG AAGCTGTCAC AGTTGAGTTC AAGCCAATTA CATTAAAAGC TGGAGAGAGC 
CGATATACCA AAGTTGCAAG TATTAGTTTT GATGCTTCAA AAGCAAGAAG ATCAGACCAG 
TTTTCTGGAA AGATCACAGT GAAGGCAAAA GAGAAAAGCT ACTCAAAACT TGAAATCCCT 
TACCAGGCTG AGGTTTTAGA AGGGTACCTG GGCTTCGACC ACACAGCCAC CCTGTTCCAC 
ATCAGAGACA GTCCTGTAGA TCCAGTGGAC AGACCCATCT ACCTCACCAA TACCTTCAAC 
TTTGCCATAC GCATTCACAA TGTGTCACTT CCCAAAGAAG CCAAGACTAT GTTCAATGTG 
CATAATTTCA GCACTCCAGT ACTCATCCCT CCCCATGAGT CCCGCTACAT TTTCTCTCTT 
CTTTTTCGAC CAGTACGACC CTCCATTCAC ATCGACAGCA ACATCCTCCT CATCACCAAT 
GCTTCTAAAT TTCACCTGCC AGTTCGGGCC TACACAGGCT TCTTGGAGCC GGTTGTTCTT 
CCTCCCTGTC TTCAAGAGCA CATCTTAGAC TTTGGTGTTC TTAGTGCCAC AGACACCAGC 
AGTTTGGTGT TTGTTGTGGT CAACAGCAAT CCAATTGAGT TGGAGATAAA GTCATGGCTG 
GTGACAGGAG ACAGTCTGTC CATGGAATTG CTTAACACAG AAAGAGGGAA CAGAAGTATA 
GCACTAAGCA GAATTCAAGA GCTGCAGAAC ACATCAGCTG CCCAATACAA AACTGTCATT 
TTAGCCTCAG GATATTATGC AGCTTTTAAA GTCACTTTGG TGGCTAAAGC TCTAGAGGGA 
ATTTATGATG GAGCCGTACA CATTACAACA GATTATGAGA TATTGACAAT CCCTGTTAAA 
GCTCTTATAG CTGTAGGAAC ATTAAGCTGT TCACCCAAAC ATATCATTCT TCCTGATGCA 
TTCCCGGGGA AAGTAGTCCA CCAAGGGTTG AGTATACAGA GCTCATTCTC ACAGAAAGTA 
AGGCTTCAGC AGATTCGCTC TCTCACAGAG GACGTCCGGT TTCAGTACAA ACGAGTTCGC 
AATAATAAAG TGGAGTTGGA ACCCAGGCGA AAGTCCAAGA TAGCGAACAT TTATTTTGAT 
GCCAGCTTGC AATGTGGCGA TCACTGTTAT GTGGGATTAC CGTTTGTATT GAAATCGGAG 
TCTAGGCCTC ATGGTTTAGT GATGCAGGAG GACATGTGGG ACGCTGATGT AGATCTCCAC 
CAGCGGCTCC TAAAAAGATG GCGAGAGCTC AAGGAGCAAT CAGGCCATGA GGTCGAAGCC 
TTCTTTGAAG TGGACACAGA TCTGCAGAAA AATGTGCAGG CCAAGATTTC AGCAAAGCTC 
AAATGGCCCT CGCTGCTCAA CTCTACCCTA CAACAGATCC ATTTTCCTCT CACAAACACA 
AACAGCTCAT CTGAGGAAGA AGTGATTCTA GAAAACTCTG CTGATGTCCC AGTCTACGTT 
CAGGTTCTTC CTCTGGCTTT ATACCCCAAT CCCTCAGTGT TCACTGGCAA ACTTGCTGAC 
AGGTTTCCTC TTGGAAAGTT ATCAAACATA AACCTGGACA CAAATACACT GGAATTCCTG 
GTCCAGAAAA ATCATTCTTC AGTGATGAAG AGTTCCACAG GTTTTGTTGA AGGATCGACA 
CGGCCCTATG TATATAACAT GCTCCTTATG CCTGGGGAGG TGAAATCCTT CAATGTAAGG 
TTTAGACCCA CCACCAATCA CAGTGTCTCT TCTCTGCTTG TCGTCAGGAA CAACTTGACG 
ATCGTAGACT TGATCATGGT TCATGGTCAG GGCACCACAG AGAGTCTGAG ATTGGCCGGT 
AAAGCCCCAG GTCTCGGCAG CTCTCTGAGG TTTAAACTTA CCGAGTCATT CCTTAAAGAC 
TGTTCAGAAG TTAAAGCCAA GGAACCAAAC TTCACTCTTC GAAGGACTTT TAGGCTGGAG 
AATACGGGCC TGCTTCCGAT TCACATTCGG TCTGTGGAGA TCAGTGGCAA TGCATGTGAG 
GGTTATGGCT TCAAAGTTCT CAACTGTGAA GAATTTGCAC TCAAACCAAA CACTTCGAAA 
GACCTCATCA TACTGTTTAC TCCAGATTTC ACATCATCCA GAGTGATCCG CGAGCTGAAG 
TTGGTGACTG CTGGGGGCTC AGTGTTTGCC TTTGTGCTGA ATGCCTCATT GCCATATCAC 
ATGCTGGCAG TGTGTGCTGA GATGCTTCCC CGACCCAACT GGGAGCTTGA GCTCTACATC 
ATCGTCTCCC TCGTTATGAG CTCCATGTTC CTGCTGGTCA TCGCTACAGC CTATCTGGAA 
GCCCAGGGAA TATGGGAGCC TTTCAAGCGA CGGCTGTCTG TGGAATCCAA CTCTCCCATG 
GAGACTGGAA AACCATTTGA TCTCAGGGAA ATTGTACGAA TTCAAAGTGA TCCAAACTTG 
AATGATTTTG CTGACTCAAA CCAGAACTCG ACGAGAGGAA TGTACGGGTC AAGCATAGGT 
GGGCGGGCTG GGGGTCGGCA AAGTGGCTCA CACTCTAGTT CCAACAGCAA CGGTGTTTTG 
CCAGATAGCA AGCCCAGATC TGGCTCAGGC CGCTCTGGAT CTATGCAATC CTCTTCCACC 
TTTTCACTCA AAGATAATGG TCCAGGACTC AACCAGGTGA CTAACCGAAA AGCTCGAAGT 
TCCAAACAGC AGCAGCAGGG CTCAGGCCTT CTTGAGGATC TGCCAAGTAC AGGACCCTCA 
CAGTCTAACA GCGCAAGGGT CAACAGTACA GAAGATCAGG ATTATAATCG ACTCCTGGTA 
GCCATGGATA AAGACCTGGA CAGGCCTGAG TCTCCTGCCA TTAATGTGTC TTCAGAGCAC 
AATTCACAAC AGTCTTCACA AAACAAAGTG CTTGAATCAA AAGGGAAGAA TGAAACAAAA 
GTAAAGAATA AAACTAAAGC GCTAAAGAAG AAAGAGGAGA AAGAAAGGAA AACAAAGGGA 
AAGACTCAAG GGGAGGATTT TAAAGAGCCC TTGGCAGACA ATGACGACAG CTCCTCCACT 
ACCACAGAGA CCTCTAACCC TGATGTGGAG GCCAATATTA AAGAGGAGCC AGTGAAGGTG 
AAAGGAAAAA CTGTGGCAAT AGACAATGGA AAAGAAAGAG AAGAAATTAC TCCCTCCCCT 
ACTAAACCAA AAAGTAAAAA ACAACCTACA ACAAAGAAGG AGAACCCTGT GGAAAAATCC 
AGTTCTTTAG AGCTCCCCTA TGTGACACCA CTGGAAAAAC AGGACAAGCG GAAGAACTTC 
AAAACACTTG CCAGTACAGC AATTCCTGGA GTATCCAAAC CACGAGCTTT GAGCAAATTA 
GTCAATGCAA AGATGGGTGA TGACCGGCCA TCTCCACTTG GAAAGTTTGT GTCTAATGGC 
TCCATGCCAG AGTTGGGTCA TAGCAGTGGA TCAGAAGGAG AAAAAGAGTC TCCTCCACCA 
GAATGGGATG TTCCTCTCAA CAAAAATGGC AACCAAGCAG ACAGTCTCCA GCAGATATCT 
ATGCAGACCA TGCATGCAGA CCCCTTCCTG AAAGGACCCA GTTCAGTCAT CAACAGAACC 
TGTTCCCCAC CTCCAACCTC CCCCAGCCTG GTGCCTCATG GAAGCTACAG CAGCATGATG 
CACCCCAGCA GTGAAATTAA TTTAAAGAAG GCTCCAGGGA ATAAGCTCAC CAAGTCAGCC 
TCTCTGCCTG GTCAAAATGG AAATCCAACT TTTGCCGCTG TGGCTGCTGG CTACGATAAA 
AGCCCTGGTG GCAGTGGCTG TGCTAGTAAA GCTGACGGTT TGGGGAAGGT GGCACTGCCT 
CACATGACAT CAGTGGAGAG TGACGGATCT GACAGCTCTG GACTCTGGAG CCCAGTAGAC 
TCCACCAGCA GCCCCAGTTT TAACCAAGTT AATGCTTTTT CTGCTTTTGG ACCAAACAAC 
CCACTCAACC TCTCTGGAGT TTTCAGAGGC ATGACTGTTC CCAAGAGTTC AGAATCCCAG 
CAGAGTTGGC CTGAGTACAC TCCAGTGTCT CCATCCATCT GGGATACAGA GACTGATAAT 
TCCTGGACCA CCATCACTGG ATCCCCAGCC ACTCCAACAA CTTCCATTTT GGGGAGCGCA 
CGCAGTCCCT GGTCTTTCAG CAGTTCCATC TGGTCCACTG GCAACGACTC CACCCTTCAC 
TCCTTCAGTC CTCCTGCAAC ATCCACCCTC CCTGACCTGG TCAGTCACAC TGCACCTCCC 
ACACCTGCGC CAAACGACAT GGGCCGCACC TACAATCCCT GGAGTATGGG CTGGTCTCCC 
TCCCTCAACC GCTGGAACTC TGAGCCCTGG CCAAACAGCC CAGACAACAA CAATGGCATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332821.1
CDS	37..5439
Translation	MASKDTTQSSLHPTRTKSACVGLLRLLFIAFIHVAQANKQAFIQSDNILEVLHFGDGSLLQTESDIDLSSYRQKSASSQRGNSRPIRFEPPMLDFHEQPVGMPKMEKVYLHNPSSEEISLISISATTSHFHASFFQNRIIPPGGNTSFDVVFLARVVGNVENTLFINTSHHGVFTYQVFGVGVPNPYRLRPFVGARVPVNSSFSPLISIFNPHSEPLQVVEMYTSGGDLHLELPTGQQGGTKKLWEIAPFETKGVMRASFSSRDADNHTAFIRIKTNASNEDEFIILPVEVEVTTAPGIYSSTEMLDFGTLRSQDRPKQLNLHLLNSGAKDVSIMSVRPTPSNEAVTVEFKPITLKAGESRYTKVASISFDASKARRSDQFSGKITVKAKEKSYSKLEIPYQAEVLEGYLGFDHTATLFHIRDSPVDPVDRPIYLTNTFNFAIRIHNVSLPKEAKTMFNVHNFSTPVLIPPHESRYIFSLLFRPVRPSIHIDSNILLITNASKFHLPVRAYTGFLEPVVLPPCLQEHILDFGVLSATDTSSLVFVVVNSNPIELEIKSWLVTGDSLSMELLNTERGNRSIALSRIQELQNTSAAQYKTVILASGYYAAFKVTLVAKALEGIYDGAVHITTDYEILTIPVKALIAVGTLSCSPKHIILPDAFPGKVVHQGLSIQSSFSQKVRLQQIRSLTEDVRFQYKRVRNNKVELEPRRKSKIANIYFDASLQCGDHCYVGLPFVLKSESRPHGLVMQEDMWDADVDLHQRLLKRWRELKEQSGHEVEAFFEVDTDLQKNVQAKISAKLKWPSLLNSTLQQIHFPLTNTNSSSEEEVILENSADVPVYVQVLPLALYPNPSVFTGKLADRFPLGKLSNINLDTNTLEFLVQKNHSSVMKSSTGFVEGSTRPYVYNMLLMPGEVKSFNVRFRPTTNHSVSSLLVVRNNLTIVDLIMVHGQGTTESLRLAGKAPGLGSSLRFKLTESFLKDCSEVKAKEPNFTLRRTFRLENTGLLPIHIRSVEISGNACEGYGFKVLNCEEFALKPNTSKDLIILFTPDFTSSRVIRELKLVTAGGSVFAFVLNASLPYHMLAVCAEMLPRPNWELELYIIVSLVMSSMFLLVIATAYLEAQGIWEPFKRRLSVESNSPMETGKPFDLREIVRIQSDPNLNDFADSNQNSTRGMYGSSIGGRAGGRQSGSHSSSNSNGVLPDSKPRSGSGRSGSMQSSSTFSLKDNGPGLNQVTNRKARSSKQQQQGSGLLEDLPSTGPSQSNSARVNSTEDQDYNRLLVAMDKDLDRPESPAINVSSEHNSQQSSQNKVLESKGKNETKVKNKTKALKKKEEKERKTKGKTQGEDFKEPLADNDDSSSTTTETSNPDVEANIKEEPVKVKGKTVAIDNGKEREEITPSPTKPKSKKQPTTKKENPVEKSSSLELPYVTPLEKQDKRKNFKTLASTAIPGVSKPRALSKLVNAKMGDDRPSPLGKFVSNGSMPELGHSSGSEGEKESPPPEWDVPLNKNGNQADSLQQISMQTMHADPFLKGPSSVINRTCSPPPTSPSLVPHGSYSSMMHPSSEINLKKAPGNKLTKSASLPGQNGNPTFAAVAAGYDKSPGGSGCASKADGLGKVALPHMTSVESDGSDSSGLWSPVDSTSSPSFNQVNAFSAFGPNNPLNLSGVFRGMTVPKSSESQQSWPEYTPVSPSIWDTETDNSWTTITGSPATPTTSILGSARSPWSFSSSIWSTGNDSTLHSFSPPATSTLPDLVSHTAPPTPAPNDMGRTYNPWSMGWSPSLNRWNSEPWPNSPDNNNGI

Target ORF information:

RefSeq Version	XM_021477146.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane protein 131 (tmem131), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477146.1

ORF Insert Sequence:

ATGGCGAGCA AGGACACAAC ACAGAGTTCC CTACATCCGA CACGAACAAA ATCAGCATGT 
GTTGGACTTC TGCGCCTTTT ATTTATTGCA TTTATTCACG TGGCACAGGC GAACAAACAA 
GCTTTCATAC AGTCAGACAA CATCCTGGAG GTACTACACT TTGGGGATGG AAGCCTCTTG 
CAGACAGAGT CTGATATTGA CCTTAGCTCA TATCGTCAAA AAAGTGCATC CTCTCAGCGG 
GGGAACAGTA GGCCCATACG CTTTGAGCCT CCAATGCTGG ACTTTCATGA ACAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTTTACTTA CACAATCCAA GTTCAGAAGA AATAAGTTTA 
ATATCAATAT CAGCAACAAC ATCACATTTT CATGCATCCT TTTTTCAGAA TAGGATAATT 
CCACCTGGAG GCAACACCTC ATTTGATGTG GTGTTTCTAG CTAGAGTAGT AGGGAATGTA 
GAAAATACCT TATTTATTAA TACATCGCAT CATGGAGTGT TTACATACCA GGTGTTTGGT 
GTGGGCGTCC CCAATCCCTA CAGACTCAGG CCCTTTGTAG GGGCAAGAGT TCCAGTAAAT 
AGCAGCTTCT CCCCACTCAT AAGCATCTTC AACCCACACA GTGAACCACT GCAGGTTGTA 
GAGATGTACA CCAGTGGAGG AGACCTACAT TTAGAGCTGC CTACTGGACA GCAGGGTGGC 
ACCAAAAAAT TATGGGAGAT CGCTCCTTTT GAGACGAAGG GTGTGATGAG AGCCAGCTTC 
TCCTCTCGAG ACGCTGACAA CCACACAGCT TTCATCAGAA TAAAAACAAA TGCGTCCAAC 
GAGGACGAGT TTATCATCCT CCCTGTGGAG GTTGAAGTCA CCACAGCACC TGGTATATAC 
TCCTCCACAG AGATGCTGGA CTTTGGTACA CTTCGATCAC AAGATCGACC AAAACAACTG 
AATTTGCATC TGTTAAATTC AGGAGCAAAA GATGTGTCGA TCATGAGTGT TAGGCCAACT 
CCATCGAACG AAGCTGTCAC AGTTGAGTTC AAGCCAATTA CATTAAAAGC TGGAGAGAGC 
CGATATACCA AAGTTGCAAG TATTAGTTTT GATGCTTCAA AAGCAAGAAG ATCAGACCAG 
TTTTCTGGAA AGATCACAGT GAAGGCAAAA GAGAAAAGCT ACTCAAAACT TGAAATCCCT 
TACCAGGCTG AGGTTTTAGA AGGGTACCTG GGCTTCGACC ACACAGCCAC CCTGTTCCAC 
ATCAGAGACA GTCCTGTAGA TCCAGTGGAC AGACCCATCT ACCTCACCAA TACCTTCAAC 
TTTGCCATAC GCATTCACAA TGTGTCACTT CCCAAAGAAG CCAAGACTAT GTTCAATGTG 
CATAATTTCA GCACTCCAGT ACTCATCCCT CCCCATGAGT CCCGCTACAT TTTCTCTCTT 
CTTTTTCGAC CAGTACGACC CTCCATTCAC ATCGACAGCA ACATCCTCCT CATCACCAAT 
GCTTCTAAAT TTCACCTGCC AGTTCGGGCC TACACAGGCT TCTTGGAGCC GGTTGTTCTT 
CCTCCCTGTC TTCAAGAGCA CATCTTAGAC TTTGGTGTTC TTAGTGCCAC AGACACCAGC 
AGTTTGGTGT TTGTTGTGGT CAACAGCAAT CCAATTGAGT TGGAGATAAA GTCATGGCTG 
GTGACAGGAG ACAGTCTGTC CATGGAATTG CTTAACACAG AAAGAGGGAA CAGAAGTATA 
GCACTAAGCA GAATTCAAGA GCTGCAGAAC ACATCAGCTG CCCAATACAA AACTGTCATT 
TTAGCCTCAG GATATTATGC AGCTTTTAAA GTCACTTTGG TGGCTAAAGC TCTAGAGGGA 
ATTTATGATG GAGCCGTACA CATTACAACA GATTATGAGA TATTGACAAT CCCTGTTAAA 
GCTCTTATAG CTGTAGGAAC ATTAAGCTGT TCACCCAAAC ATATCATTCT TCCTGATGCA 
TTCCCGGGGA AAGTAGTCCA CCAAGGGTTG AGTATACAGA GCTCATTCTC ACAGAAAGTA 
AGGCTTCAGC AGATTCGCTC TCTCACAGAG GACGTCCGGT TTCAGTACAA ACGAGTTCGC 
AATAATAAAG TGGAGTTGGA ACCCAGGCGA AAGTCCAAGA TAGCGAACAT TTATTTTGAT 
GCCAGCTTGC AATGTGGCGA TCACTGTTAT GTGGGATTAC CGTTTGTATT GAAATCGGAG 
TCTAGGCCTC ATGGTTTAGT GATGCAGGAG GACATGTGGG ACGCTGATGT AGATCTCCAC 
CAGCGGCTCC TAAAAAGATG GCGAGAGCTC AAGGAGCAAT CAGGCCATGA GGTCGAAGCC 
TTCTTTGAAG TGGACACAGA TCTGCAGAAA AATGTGCAGG CCAAGATTTC AGCAAAGCTC 
AAATGGCCCT CGCTGCTCAA CTCTACCCTA CAACAGATCC ATTTTCCTCT CACAAACACA 
AACAGCTCAT CTGAGGAAGA AGTGATTCTA GAAAACTCTG CTGATGTCCC AGTCTACGTT 
CAGGTTCTTC CTCTGGCTTT ATACCCCAAT CCCTCAGTGT TCACTGGCAA ACTTGCTGAC 
AGGTTTCCTC TTGGAAAGTT ATCAAACATA AACCTGGACA CAAATACACT GGAATTCCTG 
GTCCAGAAAA ATCATTCTTC AGTGATGAAG AGTTCCACAG GTTTTGTTGA AGGATCGACA 
CGGCCCTATG TATATAACAT GCTCCTTATG CCTGGGGAGG TGAAATCCTT CAATGTAAGG 
TTTAGACCCA CCACCAATCA CAGTGTCTCT TCTCTGCTTG TCGTCAGGAA CAACTTGACG 
ATCGTAGACT TGATCATGGT TCATGGTCAG GGCACCACAG AGAGTCTGAG ATTGGCCGGT 
AAAGCCCCAG GTCTCGGCAG CTCTCTGAGG TTTAAACTTA CCGAGTCATT CCTTAAAGAC 
TGTTCAGAAG TTAAAGCCAA GGAACCAAAC TTCACTCTTC GAAGGACTTT TAGGCTGGAG 
AATACGGGCC TGCTTCCGAT TCACATTCGG TCTGTGGAGA TCAGTGGCAA TGCATGTGAG 
GGTTATGGCT TCAAAGTTCT CAACTGTGAA GAATTTGCAC TCAAACCAAA CACTTCGAAA 
GACCTCATCA TACTGTTTAC TCCAGATTTC ACATCATCCA GAGTGATCCG CGAGCTGAAG 
TTGGTGACTG CTGGGGGCTC AGTGTTTGCC TTTGTGCTGA ATGCCTCATT GCCATATCAC 
ATGCTGGCAG TGTGTGCTGA GATGCTTCCC CGACCCAACT GGGAGCTTGA GCTCTACATC 
ATCGTCTCCC TCGTTATGAG CTCCATGTTC CTGCTGGTCA TCGCTACAGC CTATCTGGAA 
GCCCAGGGAA TATGGGAGCC TTTCAAGCGA CGGCTGTCTG TGGAATCCAA CTCTCCCATG 
GAGACTGGAA AACCATTTGA TCTCAGGGAA ATTGTACGAA TTCAAAGTGA TCCAAACTTG 
AATGATTTTG CTGACTCAAA CCAGAACTCG ACGAGAGGAA TGTACGGGTC AAGCATAGGT 
GGGCGGGCTG GGGGTCGGCA AAGTGGCTCA CACTCTAGTT CCAACAGCAA CGGTGTTTTG 
CCAGATAGCA AGCCCAGATC TGGCTCAGGC CGCTCTGGAT CTATGCAATC CTCTTCCACC 
TTTTCACTCA AAGATAATGG TCCAGGACTC AACCAGGTGA CTAACCGAAA AGCTCGAAGT 
TCCAAACAGC AGCAGCAGGG CTCAGGCCTT CTTGAGGATC TGCCAAGTAC AGGACCCTCA 
CAGTCTAACA GCGCAAGGGT CAACAGTACA GAAGATCAGG ATTATAATCG ACTCCTGGTA 
GCCATGGATA AAGACCTGGA CAGGCCTGAG TCTCCTGCCA TTAATGTGTC TTCAGAGCAC 
AATTCACAAC AGTCTTCACA AAACAAAGTG CTTGAATCAA AAGGGAAGAA TGAAACAAAA 
GTAAAGAATA AAACTAAAGC GCTAAAGAAG AAAGAGGAGA AAGAAAGGAA AACAAAGGGA 
AAGACTCAAG GGGAGGATTT TAAAGAGCCC TTGGCAGACA ATGACGACAG CTCCTCCACT 
ACCACAGAGA CCTCTAACCC TGATGTGGAG GCCAATATTA AAGAGGAGCC AGTGAAGGTG 
AAAGGAAAAA CTGTGGCAAT AGACAATGGA AAAGAAAGAG AAGAAATTAC TCCCTCCCCT 
ACTAAACCAA AAAGTAAAAA ACAACCTACA ACAAAGAAGG AGAACCCTGT GGAAAAATCC 
AGTTCTTTAG AGCTCCCCTA TGTGACACCA CTGGAAAAAC AGGACAAGCG GAAGAACTTC 
AAAACACTTG CCAGTACAGC AATTCCTGGA GTATCCAAAC CACGAGCTTT GAGCAAATTA 
GTCAATGCAA AGATGGGTGA TGACCGGCCA TCTCCACTTG GAAAGTTTGT GTCTAATGGC 
TCCATGCCAG AGTTGGGTCA TAGCAGTGGA TCAGAAGGAG AAAAAGAGTC TCCTCCACCA 
GAATGGGATG TTCCTCTCAA CAAAAATGGC AACCAAGCAG ACAGTCTCCA GCAGATATCT 
ATGCAGACCA TGCATGCAGA CCCCTTCCTG AAAGGACCCA GTTCAGTCAT CAACAGAACC 
TGTTCCCCAC CTCCAACCTC CCCCAGCCTG GTGCCTCATG GAAGCTACAG CAGCATGATG 
CACCCCAGCA GTGAAATTAA TTTAAAGAAG GCTCCAGGGA ATAAGCTCAC CAAGTCAGCC 
TCTCTGCCTG GTCAAAATGG AAATCCAACT TTTGCCGCTG TGGCTGCTGG CTACGATAAA 
AGCCCTGGTG GCAGTGGCTG TGCTAGTAAA GCTGACGGTT TGGGGAAGGT GGCACTGCCT 
CACATGACAT CAGTGGAGAG TGACGGATCT GACAGCTCTG GACTCTGGAG CCCAGTAGAC 
TCCACCAGCA GCCCCAGTTT TAACCAAGTT AATGCTTTTT CTGCTTTTGG ACCAAACAAC 
CCACTCAACC TCTCTGGAGT TTTCAGAGGC ATGACTGTTC CCAAGAGTTC AGAATCCCAG 
CAGAGTTGGC CTGAGTACAC TCCAGTGTCT CCATCCATCT GGGATACAGA GACTGATAAT 
TCCTGGACCA CCATCACTGG ATCCCCAGCC ACTCCAACAA CTTCCATTTT GGGGAGCGCA 
CGCAGTCCCT GGTCTTTCAG CAGTTCCATC TGGTCCACTG GCAACGACTC CACCCTTCAC 
TCCTTCAGTC CTCCTGCAAC ATCCACCCTC CCTGACCTGG TCAGTCACAC TGCACCTCCC 
ACACCTGCGC CAAACGACAT GGGCCGCACC TACAATCCCT GGAGTATGGG CTGGTCTCCC 
TCCCTCAACC GCTGGAACTC TGAGCCCTGG CCAAACAGCC CAGACAACAA CAATGGCATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tmem131 ( XM_005165961.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166018.1 Danio rerio transmembrane protein 131 (tmem131), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa11897
Clone ID Related Accession (Same CDS sequence)	XM_021477146.1 , XM_005165961.3 , XM_005165961.4
Accession Version	XM_005165961.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transmembrane protein 131 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165961.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCA AGGACACAAC ACAGAGTTCC CTACATCCGA CACGAACAAA ATCAGCATGT 
GTTGGACTTC TGCGCCTTTT ATTTATTGCA TTTATTCACG TGGCACAGGC GAACAAACAA 
GCTTTCATAC AGTCAGACAA CATCCTGGAG GTACTACACT TTGGGGATGG AAGCCTCTTG 
CAGACAGAGT CTGATATTGA CCTTAGCTCA TATCGTCAAA AAAGTGCATC CTCTCAGCGG 
GGGAACAGTA GGCCCATACG CTTTGAGCCT CCAATGCTGG ACTTTCATGA ACAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTTTACTTA CACAATCCAA GTTCAGAAGA AATAAGTTTA 
ATATCAATAT CAGCAACAAC ATCACATTTT CATGCATCCT TTTTTCAGAA TAGGATAATT 
CCACCTGGAG GCAACACCTC ATTTGATGTG GTGTTTCTAG CTAGAGTAGT AGGGAATGTA 
GAAAATACCT TATTTATTAA TACATCGCAT CATGGAGTGT TTACATACCA GGTGTTTGGT 
GTGGGCGTCC CCAATCCCTA CAGACTCAGG CCCTTTGTAG GGGCAAGAGT TCCAGTAAAT 
AGCAGCTTCT CCCCACTCAT AAGCATCTTC AACCCACACA GTGAACCACT GCAGGTTGTA 
GAGATGTACA CCAGTGGAGG AGACCTACAT TTAGAGCTGC CTACTGGACA GCAGGGTGGC 
ACCAAAAAAT TATGGGAGAT CGCTCCTTTT GAGACGAAGG GTGTGATGAG AGCCAGCTTC 
TCCTCTCGAG ACGCTGACAA CCACACAGCT TTCATCAGAA TAAAAACAAA TGCGTCCAAC 
GAGGACGAGT TTATCATCCT CCCTGTGGAG GTTGAAGTCA CCACAGCACC TGGTATATAC 
TCCTCCACAG AGATGCTGGA CTTTGGTACA CTTCGATCAC AAGATCGACC AAAACAACTG 
AATTTGCATC TGTTAAATTC AGGAGCAAAA GATGTGTCGA TCATGAGTGT TAGGCCAACT 
CCATCGAACG AAGCTGTCAC AGTTGAGTTC AAGCCAATTA CATTAAAAGC TGGAGAGAGC 
CGATATACCA AAGTTGCAAG TATTAGTTTT GATGCTTCAA AAGCAAGAAG ATCAGACCAG 
TTTTCTGGAA AGATCACAGT GAAGGCAAAA GAGAAAAGCT ACTCAAAACT TGAAATCCCT 
TACCAGGCTG AGGTTTTAGA AGGGTACCTG GGCTTCGACC ACACAGCCAC CCTGTTCCAC 
ATCAGAGACA GTCCTGTAGA TCCAGTGGAC AGACCCATCT ACCTCACCAA TACCTTCAAC 
TTTGCCATAC GCATTCACAA TGTGTCACTT CCCAAAGAAG CCAAGACTAT GTTCAATGTG 
CATAATTTCA GCACTCCAGT ACTCATCCCT CCCCATGAGT CCCGCTACAT TTTCTCTCTT 
CTTTTTCGAC CAGTACGACC CTCCATTCAC ATCGACAGCA ACATCCTCCT CATCACCAAT 
GCTTCTAAAT TTCACCTGCC AGTTCGGGCC TACACAGGCT TCTTGGAGCC GGTTGTTCTT 
CCTCCCTGTC TTCAAGAGCA CATCTTAGAC TTTGGTGTTC TTAGTGCCAC AGACACCAGC 
AGTTTGGTGT TTGTTGTGGT CAACAGCAAT CCAATTGAGT TGGAGATAAA GTCATGGCTG 
GTGACAGGAG ACAGTCTGTC CATGGAATTG CTTAACACAG AAAGAGGGAA CAGAAGTATA 
GCACTAAGCA GAATTCAAGA GCTGCAGAAC ACATCAGCTG CCCAATACAA AACTGTCATT 
TTAGCCTCAG GATATTATGC AGCTTTTAAA GTCACTTTGG TGGCTAAAGC TCTAGAGGGA 
ATTTATGATG GAGCCGTACA CATTACAACA GATTATGAGA TATTGACAAT CCCTGTTAAA 
GCTCTTATAG CTGTAGGAAC ATTAAGCTGT TCACCCAAAC ATATCATTCT TCCTGATGCA 
TTCCCGGGGA AAGTAGTCCA CCAAGGGTTG AGTATACAGA GCTCATTCTC ACAGAAAGTA 
AGGCTTCAGC AGATTCGCTC TCTCACAGAG GACGTCCGGT TTCAGTACAA ACGAGTTCGC 
AATAATAAAG TGGAGTTGGA ACCCAGGCGA AAGTCCAAGA TAGCGAACAT TTATTTTGAT 
GCCAGCTTGC AATGTGGCGA TCACTGTTAT GTGGGATTAC CGTTTGTATT GAAATCGGAG 
TCTAGGCCTC ATGGTTTAGT GATGCAGGAG GACATGTGGG ACGCTGATGT AGATCTCCAC 
CAGCGGCTCC TAAAAAGATG GCGAGAGCTC AAGGAGCAAT CAGGCCATGA GGTCGAAGCC 
TTCTTTGAAG TGGACACAGA TCTGCAGAAA AATGTGCAGG CCAAGATTTC AGCAAAGCTC 
AAATGGCCCT CGCTGCTCAA CTCTACCCTA CAACAGATCC ATTTTCCTCT CACAAACACA 
AACAGCTCAT CTGAGGAAGA AGTGATTCTA GAAAACTCTG CTGATGTCCC AGTCTACGTT 
CAGGTTCTTC CTCTGGCTTT ATACCCCAAT CCCTCAGTGT TCACTGGCAA ACTTGCTGAC 
AGGTTTCCTC TTGGAAAGTT ATCAAACATA AACCTGGACA CAAATACACT GGAATTCCTG 
GTCCAGAAAA ATCATTCTTC AGTGATGAAG AGTTCCACAG GTTTTGTTGA AGGATCGACA 
CGGCCCTATG TATATAACAT GCTCCTTATG CCTGGGGAGG TGAAATCCTT CAATGTAAGG 
TTTAGACCCA CCACCAATCA CAGTGTCTCT TCTCTGCTTG TCGTCAGGAA CAACTTGACG 
ATCGTAGACT TGATCATGGT TCATGGTCAG GGCACCACAG AGAGTCTGAG ATTGGCCGGT 
AAAGCCCCAG GTCTCGGCAG CTCTCTGAGG TTTAAACTTA CCGAGTCATT CCTTAAAGAC 
TGTTCAGAAG TTAAAGCCAA GGAACCAAAC TTCACTCTTC GAAGGACTTT TAGGCTGGAG 
AATACGGGCC TGCTTCCGAT TCACATTCGG TCTGTGGAGA TCAGTGGCAA TGCATGTGAG 
GGTTATGGCT TCAAAGTTCT CAACTGTGAA GAATTTGCAC TCAAACCAAA CACTTCGAAA 
GACCTCATCA TACTGTTTAC TCCAGATTTC ACATCATCCA GAGTGATCCG CGAGCTGAAG 
TTGGTGACTG CTGGGGGCTC AGTGTTTGCC TTTGTGCTGA ATGCCTCATT GCCATATCAC 
ATGCTGGCAG TGTGTGCTGA GATGCTTCCC CGACCCAACT GGGAGCTTGA GCTCTACATC 
ATCGTCTCCC TCGTTATGAG CTCCATGTTC CTGCTGGTCA TCGCTACAGC CTATCTGGAA 
GCCCAGGGAA TATGGGAGCC TTTCAAGCGA CGGCTGTCTG TGGAATCCAA CTCTCCCATG 
GAGACTGGAA AACCATTTGA TCTCAGGGAA ATTGTACGAA TTCAAAGTGA TCCAAACTTG 
AATGATTTTG CTGACTCAAA CCAGAACTCG ACGAGAGGAA TGTACGGGTC AAGCATAGGT 
GGGCGGGCTG GGGGTCGGCA AAGTGGCTCA CACTCTAGTT CCAACAGCAA CGGTGTTTTG 
CCAGATAGCA AGCCCAGATC TGGCTCAGGC CGCTCTGGAT CTATGCAATC CTCTTCCACC 
TTTTCACTCA AAGATAATGG TCCAGGACTC AACCAGGTGA CTAACCGAAA AGCTCGAAGT 
TCCAAACAGC AGCAGCAGGG CTCAGGCCTT CTTGAGGATC TGCCAAGTAC AGGACCCTCA 
CAGTCTAACA GCGCAAGGGT CAACAGTACA GAAGATCAGG ATTATAATCG ACTCCTGGTA 
GCCATGGATA AAGACCTGGA CAGGCCTGAG TCTCCTGCCA TTAATGTGTC TTCAGAGCAC 
AATTCACAAC AGTCTTCACA AAACAAAGTG CTTGAATCAA AAGGGAAGAA TGAAACAAAA 
GTAAAGAATA AAACTAAAGC GCTAAAGAAG AAAGAGGAGA AAGAAAGGAA AACAAAGGGA 
AAGACTCAAG GGGAGGATTT TAAAGAGCCC TTGGCAGACA ATGACGACAG CTCCTCCACT 
ACCACAGAGA CCTCTAACCC TGATGTGGAG GCCAATATTA AAGAGGAGCC AGTGAAGGTG 
AAAGGAAAAA CTGTGGCAAT AGACAATGGA AAAGAAAGAG AAGAAATTAC TCCCTCCCCT 
ACTAAACCAA AAAGTAAAAA ACAACCTACA ACAAAGAAGG AGAACCCTGT GGAAAAATCC 
AGTTCTTTAG AGCTCCCCTA TGTGACACCA CTGGAAAAAC AGGACAAGCG GAAGAACTTC 
AAAACACTTG CCAGTACAGC AATTCCTGGA GTATCCAAAC CACGAGCTTT GAGCAAATTA 
GTCAATGCAA AGATGGGTGA TGACCGGCCA TCTCCACTTG GAAAGTTTGT GTCTAATGGC 
TCCATGCCAG AGTTGGGTCA TAGCAGTGGA TCAGAAGGAG AAAAAGAGTC TCCTCCACCA 
GAATGGGATG TTCCTCTCAA CAAAAATGGC AACCAAGCAG ACAGTCTCCA GCAGATATCT 
ATGCAGACCA TGCATGCAGA CCCCTTCCTG AAAGGACCCA GTTCAGTCAT CAACAGAACC 
TGTTCCCCAC CTCCAACCTC CCCCAGCCTG GTGCCTCATG GAAGCTACAG CAGCATGATG 
CACCCCAGCA GTGAAATTAA TTTAAAGAAG GCTCCAGGGA ATAAGCTCAC CAAGTCAGCC 
TCTCTGCCTG GTCAAAATGG AAATCCAACT TTTGCCGCTG TGGCTGCTGG CTACGATAAA 
AGCCCTGGTG GCAGTGGCTG TGCTAGTAAA GCTGACGGTT TGGGGAAGGT GGCACTGCCT 
CACATGACAT CAGTGGAGAG TGACGGATCT GACAGCTCTG GACTCTGGAG CCCAGTAGAC 
TCCACCAGCA GCCCCAGTTT TAACCAAGTT AATGCTTTTT CTGCTTTTGG ACCAAACAAC 
CCACTCAACC TCTCTGGAGT TTTCAGAGGC ATGACTGTTC CCAAGAGTTC AGAATCCCAG 
CAGAGTTGGC CTGAGTACAC TCCAGTGTCT CCATCCATCT GGGATACAGA GACTGATAAT 
TCCTGGACCA CCATCACTGG ATCCCCAGCC ACTCCAACAA CTTCCATTTT GGGGAGCGCA 
CGCAGTCCCT GGTCTTTCAG CAGTTCCATC TGGTCCACTG GCAACGACTC CACCCTTCAC 
TCCTTCAGTC CTCCTGCAAC ATCCACCCTC CCTGACCTGG TCAGTCACAC TGCACCTCCC 
ACACCTGCGC CAAACGACAT GGGCCGCACC TACAATCCCT GGAGTATGGG CTGGTCTCCC 
TCCCTCAACC GCTGGAACTC TGAGCCCTGG CCAAACAGCC CAGACAACAA CAATGGCATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166018.1
CDS	34..5436
Translation	MASKDTTQSSLHPTRTKSACVGLLRLLFIAFIHVAQANKQAFIQSDNILEVLHFGDGSLLQTESDIDLSSYRQKSASSQRGNSRPIRFEPPMLDFHEQPVGMPKMEKVYLHNPSSEEISLISISATTSHFHASFFQNRIIPPGGNTSFDVVFLARVVGNVENTLFINTSHHGVFTYQVFGVGVPNPYRLRPFVGARVPVNSSFSPLISIFNPHSEPLQVVEMYTSGGDLHLELPTGQQGGTKKLWEIAPFETKGVMRASFSSRDADNHTAFIRIKTNASNEDEFIILPVEVEVTTAPGIYSSTEMLDFGTLRSQDRPKQLNLHLLNSGAKDVSIMSVRPTPSNEAVTVEFKPITLKAGESRYTKVASISFDASKARRSDQFSGKITVKAKEKSYSKLEIPYQAEVLEGYLGFDHTATLFHIRDSPVDPVDRPIYLTNTFNFAIRIHNVSLPKEAKTMFNVHNFSTPVLIPPHESRYIFSLLFRPVRPSIHIDSNILLITNASKFHLPVRAYTGFLEPVVLPPCLQEHILDFGVLSATDTSSLVFVVVNSNPIELEIKSWLVTGDSLSMELLNTERGNRSIALSRIQELQNTSAAQYKTVILASGYYAAFKVTLVAKALEGIYDGAVHITTDYEILTIPVKALIAVGTLSCSPKHIILPDAFPGKVVHQGLSIQSSFSQKVRLQQIRSLTEDVRFQYKRVRNNKVELEPRRKSKIANIYFDASLQCGDHCYVGLPFVLKSESRPHGLVMQEDMWDADVDLHQRLLKRWRELKEQSGHEVEAFFEVDTDLQKNVQAKISAKLKWPSLLNSTLQQIHFPLTNTNSSSEEEVILENSADVPVYVQVLPLALYPNPSVFTGKLADRFPLGKLSNINLDTNTLEFLVQKNHSSVMKSSTGFVEGSTRPYVYNMLLMPGEVKSFNVRFRPTTNHSVSSLLVVRNNLTIVDLIMVHGQGTTESLRLAGKAPGLGSSLRFKLTESFLKDCSEVKAKEPNFTLRRTFRLENTGLLPIHIRSVEISGNACEGYGFKVLNCEEFALKPNTSKDLIILFTPDFTSSRVIRELKLVTAGGSVFAFVLNASLPYHMLAVCAEMLPRPNWELELYIIVSLVMSSMFLLVIATAYLEAQGIWEPFKRRLSVESNSPMETGKPFDLREIVRIQSDPNLNDFADSNQNSTRGMYGSSIGGRAGGRQSGSHSSSNSNGVLPDSKPRSGSGRSGSMQSSSTFSLKDNGPGLNQVTNRKARSSKQQQQGSGLLEDLPSTGPSQSNSARVNSTEDQDYNRLLVAMDKDLDRPESPAINVSSEHNSQQSSQNKVLESKGKNETKVKNKTKALKKKEEKERKTKGKTQGEDFKEPLADNDDSSSTTTETSNPDVEANIKEEPVKVKGKTVAIDNGKEREEITPSPTKPKSKKQPTTKKENPVEKSSSLELPYVTPLEKQDKRKNFKTLASTAIPGVSKPRALSKLVNAKMGDDRPSPLGKFVSNGSMPELGHSSGSEGEKESPPPEWDVPLNKNGNQADSLQQISMQTMHADPFLKGPSSVINRTCSPPPTSPSLVPHGSYSSMMHPSSEINLKKAPGNKLTKSASLPGQNGNPTFAAVAAGYDKSPGGSGCASKADGLGKVALPHMTSVESDGSDSSGLWSPVDSTSSPSFNQVNAFSAFGPNNPLNLSGVFRGMTVPKSSESQQSWPEYTPVSPSIWDTETDNSWTTITGSPATPTTSILGSARSPWSFSSSIWSTGNDSTLHSFSPPATSTLPDLVSHTAPPTPAPNDMGRTYNPWSMGWSPSLNRWNSEPWPNSPDNNNGI

Target ORF information:

RefSeq Version	XM_005165961.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transmembrane protein 131 (tmem131), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165961.3

ORF Insert Sequence:

ATGGCGAGCA AGGACACAAC ACAGAGTTCC CTACATCCGA CACGAACAAA ATCAGCATGT 
GTTGGACTTC TGCGCCTTTT ATTTATTGCA TTTATTCACG TGGCACAGGC GAACAAACAA 
GCTTTCATAC AGTCAGACAA CATCCTGGAG GTACTACACT TTGGGGATGG AAGCCTCTTG 
CAGACAGAGT CTGATATTGA CCTTAGCTCA TATCGTCAAA AAAGTGCATC CTCTCAGCGG 
GGGAACAGTA GGCCCATACG CTTTGAGCCT CCAATGCTGG ACTTTCATGA ACAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTTTACTTA CACAATCCAA GTTCAGAAGA AATAAGTTTA 
ATATCAATAT CAGCAACAAC ATCACATTTT CATGCATCCT TTTTTCAGAA TAGGATAATT 
CCACCTGGAG GCAACACCTC ATTTGATGTG GTGTTTCTAG CTAGAGTAGT AGGGAATGTA 
GAAAATACCT TATTTATTAA TACATCGCAT CATGGAGTGT TTACATACCA GGTGTTTGGT 
GTGGGCGTCC CCAATCCCTA CAGACTCAGG CCCTTTGTAG GGGCAAGAGT TCCAGTAAAT 
AGCAGCTTCT CCCCACTCAT AAGCATCTTC AACCCACACA GTGAACCACT GCAGGTTGTA 
GAGATGTACA CCAGTGGAGG AGACCTACAT TTAGAGCTGC CTACTGGACA GCAGGGTGGC 
ACCAAAAAAT TATGGGAGAT CGCTCCTTTT GAGACGAAGG GTGTGATGAG AGCCAGCTTC 
TCCTCTCGAG ACGCTGACAA CCACACAGCT TTCATCAGAA TAAAAACAAA TGCGTCCAAC 
GAGGACGAGT TTATCATCCT CCCTGTGGAG GTTGAAGTCA CCACAGCACC TGGTATATAC 
TCCTCCACAG AGATGCTGGA CTTTGGTACA CTTCGATCAC AAGATCGACC AAAACAACTG 
AATTTGCATC TGTTAAATTC AGGAGCAAAA GATGTGTCGA TCATGAGTGT TAGGCCAACT 
CCATCGAACG AAGCTGTCAC AGTTGAGTTC AAGCCAATTA CATTAAAAGC TGGAGAGAGC 
CGATATACCA AAGTTGCAAG TATTAGTTTT GATGCTTCAA AAGCAAGAAG ATCAGACCAG 
TTTTCTGGAA AGATCACAGT GAAGGCAAAA GAGAAAAGCT ACTCAAAACT TGAAATCCCT 
TACCAGGCTG AGGTTTTAGA AGGGTACCTG GGCTTCGACC ACACAGCCAC CCTGTTCCAC 
ATCAGAGACA GTCCTGTAGA TCCAGTGGAC AGACCCATCT ACCTCACCAA TACCTTCAAC 
TTTGCCATAC GCATTCACAA TGTGTCACTT CCCAAAGAAG CCAAGACTAT GTTCAATGTG 
CATAATTTCA GCACTCCAGT ACTCATCCCT CCCCATGAGT CCCGCTACAT TTTCTCTCTT 
CTTTTTCGAC CAGTACGACC CTCCATTCAC ATCGACAGCA ACATCCTCCT CATCACCAAT 
GCTTCTAAAT TTCACCTGCC AGTTCGGGCC TACACAGGCT TCTTGGAGCC GGTTGTTCTT 
CCTCCCTGTC TTCAAGAGCA CATCTTAGAC TTTGGTGTTC TTAGTGCCAC AGACACCAGC 
AGTTTGGTGT TTGTTGTGGT CAACAGCAAT CCAATTGAGT TGGAGATAAA GTCATGGCTG 
GTGACAGGAG ACAGTCTGTC CATGGAATTG CTTAACACAG AAAGAGGGAA CAGAAGTATA 
GCACTAAGCA GAATTCAAGA GCTGCAGAAC ACATCAGCTG CCCAATACAA AACTGTCATT 
TTAGCCTCAG GATATTATGC AGCTTTTAAA GTCACTTTGG TGGCTAAAGC TCTAGAGGGA 
ATTTATGATG GAGCCGTACA CATTACAACA GATTATGAGA TATTGACAAT CCCTGTTAAA 
GCTCTTATAG CTGTAGGAAC ATTAAGCTGT TCACCCAAAC ATATCATTCT TCCTGATGCA 
TTCCCGGGGA AAGTAGTCCA CCAAGGGTTG AGTATACAGA GCTCATTCTC ACAGAAAGTA 
AGGCTTCAGC AGATTCGCTC TCTCACAGAG GACGTCCGGT TTCAGTACAA ACGAGTTCGC 
AATAATAAAG TGGAGTTGGA ACCCAGGCGA AAGTCCAAGA TAGCGAACAT TTATTTTGAT 
GCCAGCTTGC AATGTGGCGA TCACTGTTAT GTGGGATTAC CGTTTGTATT GAAATCGGAG 
TCTAGGCCTC ATGGTTTAGT GATGCAGGAG GACATGTGGG ACGCTGATGT AGATCTCCAC 
CAGCGGCTCC TAAAAAGATG GCGAGAGCTC AAGGAGCAAT CAGGCCATGA GGTCGAAGCC 
TTCTTTGAAG TGGACACAGA TCTGCAGAAA AATGTGCAGG CCAAGATTTC AGCAAAGCTC 
AAATGGCCCT CGCTGCTCAA CTCTACCCTA CAACAGATCC ATTTTCCTCT CACAAACACA 
AACAGCTCAT CTGAGGAAGA AGTGATTCTA GAAAACTCTG CTGATGTCCC AGTCTACGTT 
CAGGTTCTTC CTCTGGCTTT ATACCCCAAT CCCTCAGTGT TCACTGGCAA ACTTGCTGAC 
AGGTTTCCTC TTGGAAAGTT ATCAAACATA AACCTGGACA CAAATACACT GGAATTCCTG 
GTCCAGAAAA ATCATTCTTC AGTGATGAAG AGTTCCACAG GTTTTGTTGA AGGATCGACA 
CGGCCCTATG TATATAACAT GCTCCTTATG CCTGGGGAGG TGAAATCCTT CAATGTAAGG 
TTTAGACCCA CCACCAATCA CAGTGTCTCT TCTCTGCTTG TCGTCAGGAA CAACTTGACG 
ATCGTAGACT TGATCATGGT TCATGGTCAG GGCACCACAG AGAGTCTGAG ATTGGCCGGT 
AAAGCCCCAG GTCTCGGCAG CTCTCTGAGG TTTAAACTTA CCGAGTCATT CCTTAAAGAC 
TGTTCAGAAG TTAAAGCCAA GGAACCAAAC TTCACTCTTC GAAGGACTTT TAGGCTGGAG 
AATACGGGCC TGCTTCCGAT TCACATTCGG TCTGTGGAGA TCAGTGGCAA TGCATGTGAG 
GGTTATGGCT TCAAAGTTCT CAACTGTGAA GAATTTGCAC TCAAACCAAA CACTTCGAAA 
GACCTCATCA TACTGTTTAC TCCAGATTTC ACATCATCCA GAGTGATCCG CGAGCTGAAG 
TTGGTGACTG CTGGGGGCTC AGTGTTTGCC TTTGTGCTGA ATGCCTCATT GCCATATCAC 
ATGCTGGCAG TGTGTGCTGA GATGCTTCCC CGACCCAACT GGGAGCTTGA GCTCTACATC 
ATCGTCTCCC TCGTTATGAG CTCCATGTTC CTGCTGGTCA TCGCTACAGC CTATCTGGAA 
GCCCAGGGAA TATGGGAGCC TTTCAAGCGA CGGCTGTCTG TGGAATCCAA CTCTCCCATG 
GAGACTGGAA AACCATTTGA TCTCAGGGAA ATTGTACGAA TTCAAAGTGA TCCAAACTTG 
AATGATTTTG CTGACTCAAA CCAGAACTCG ACGAGAGGAA TGTACGGGTC AAGCATAGGT 
GGGCGGGCTG GGGGTCGGCA AAGTGGCTCA CACTCTAGTT CCAACAGCAA CGGTGTTTTG 
CCAGATAGCA AGCCCAGATC TGGCTCAGGC CGCTCTGGAT CTATGCAATC CTCTTCCACC 
TTTTCACTCA AAGATAATGG TCCAGGACTC AACCAGGTGA CTAACCGAAA AGCTCGAAGT 
TCCAAACAGC AGCAGCAGGG CTCAGGCCTT CTTGAGGATC TGCCAAGTAC AGGACCCTCA 
CAGTCTAACA GCGCAAGGGT CAACAGTACA GAAGATCAGG ATTATAATCG ACTCCTGGTA 
GCCATGGATA AAGACCTGGA CAGGCCTGAG TCTCCTGCCA TTAATGTGTC TTCAGAGCAC 
AATTCACAAC AGTCTTCACA AAACAAAGTG CTTGAATCAA AAGGGAAGAA TGAAACAAAA 
GTAAAGAATA AAACTAAAGC GCTAAAGAAG AAAGAGGAGA AAGAAAGGAA AACAAAGGGA 
AAGACTCAAG GGGAGGATTT TAAAGAGCCC TTGGCAGACA ATGACGACAG CTCCTCCACT 
ACCACAGAGA CCTCTAACCC TGATGTGGAG GCCAATATTA AAGAGGAGCC AGTGAAGGTG 
AAAGGAAAAA CTGTGGCAAT AGACAATGGA AAAGAAAGAG AAGAAATTAC TCCCTCCCCT 
ACTAAACCAA AAAGTAAAAA ACAACCTACA ACAAAGAAGG AGAACCCTGT GGAAAAATCC 
AGTTCTTTAG AGCTCCCCTA TGTGACACCA CTGGAAAAAC AGGACAAGCG GAAGAACTTC 
AAAACACTTG CCAGTACAGC AATTCCTGGA GTATCCAAAC CACGAGCTTT GAGCAAATTA 
GTCAATGCAA AGATGGGTGA TGACCGGCCA TCTCCACTTG GAAAGTTTGT GTCTAATGGC 
TCCATGCCAG AGTTGGGTCA TAGCAGTGGA TCAGAAGGAG AAAAAGAGTC TCCTCCACCA 
GAATGGGATG TTCCTCTCAA CAAAAATGGC AACCAAGCAG ACAGTCTCCA GCAGATATCT 
ATGCAGACCA TGCATGCAGA CCCCTTCCTG AAAGGACCCA GTTCAGTCAT CAACAGAACC 
TGTTCCCCAC CTCCAACCTC CCCCAGCCTG GTGCCTCATG GAAGCTACAG CAGCATGATG 
CACCCCAGCA GTGAAATTAA TTTAAAGAAG GCTCCAGGGA ATAAGCTCAC CAAGTCAGCC 
TCTCTGCCTG GTCAAAATGG AAATCCAACT TTTGCCGCTG TGGCTGCTGG CTACGATAAA 
AGCCCTGGTG GCAGTGGCTG TGCTAGTAAA GCTGACGGTT TGGGGAAGGT GGCACTGCCT 
CACATGACAT CAGTGGAGAG TGACGGATCT GACAGCTCTG GACTCTGGAG CCCAGTAGAC 
TCCACCAGCA GCCCCAGTTT TAACCAAGTT AATGCTTTTT CTGCTTTTGG ACCAAACAAC 
CCACTCAACC TCTCTGGAGT TTTCAGAGGC ATGACTGTTC CCAAGAGTTC AGAATCCCAG 
CAGAGTTGGC CTGAGTACAC TCCAGTGTCT CCATCCATCT GGGATACAGA GACTGATAAT 
TCCTGGACCA CCATCACTGG ATCCCCAGCC ACTCCAACAA CTTCCATTTT GGGGAGCGCA 
CGCAGTCCCT GGTCTTTCAG CAGTTCCATC TGGTCCACTG GCAACGACTC CACCCTTCAC 
TCCTTCAGTC CTCCTGCAAC ATCCACCCTC CCTGACCTGG TCAGTCACAC TGCACCTCCC 
ACACCTGCGC CAAACGACAT GGGCCGCACC TACAATCCCT GGAGTATGGG CTGGTCTCCC 
TCCCTCAACC GCTGGAACTC TGAGCCCTGG CCAAACAGCC CAGACAACAA CAATGGCATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tmem131 ( XM_005165961.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166018.1 Danio rerio transmembrane protein 131 (tmem131), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa11897
Clone ID Related Accession (Same CDS sequence)	XM_021477146.1 , XM_005165961.3 , XM_005165961.4
Accession Version	XM_005165961.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transmembrane protein 131 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394647.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165961.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCA AGGACACAAC ACAGAGTTCC CTACATCCGA CACGAACAAA ATCAGCATGT 
GTTGGACTTC TGCGCCTTTT ATTTATTGCA TTTATTCACG TGGCACAGGC GAACAAACAA 
GCTTTCATAC AGTCAGACAA CATCCTGGAG GTACTACACT TTGGGGATGG AAGCCTCTTG 
CAGACAGAGT CTGATATTGA CCTTAGCTCA TATCGTCAAA AAAGTGCATC CTCTCAGCGG 
GGGAACAGTA GGCCCATACG CTTTGAGCCT CCAATGCTGG ACTTTCATGA ACAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTTTACTTA CACAATCCAA GTTCAGAAGA AATAAGTTTA 
ATATCAATAT CAGCAACAAC ATCACATTTT CATGCATCCT TTTTTCAGAA TAGGATAATT 
CCACCTGGAG GCAACACCTC ATTTGATGTG GTGTTTCTAG CTAGAGTAGT AGGGAATGTA 
GAAAATACCT TATTTATTAA TACATCGCAT CATGGAGTGT TTACATACCA GGTGTTTGGT 
GTGGGCGTCC CCAATCCCTA CAGACTCAGG CCCTTTGTAG GGGCAAGAGT TCCAGTAAAT 
AGCAGCTTCT CCCCACTCAT AAGCATCTTC AACCCACACA GTGAACCACT GCAGGTTGTA 
GAGATGTACA CCAGTGGAGG AGACCTACAT TTAGAGCTGC CTACTGGACA GCAGGGTGGC 
ACCAAAAAAT TATGGGAGAT CGCTCCTTTT GAGACGAAGG GTGTGATGAG AGCCAGCTTC 
TCCTCTCGAG ACGCTGACAA CCACACAGCT TTCATCAGAA TAAAAACAAA TGCGTCCAAC 
GAGGACGAGT TTATCATCCT CCCTGTGGAG GTTGAAGTCA CCACAGCACC TGGTATATAC 
TCCTCCACAG AGATGCTGGA CTTTGGTACA CTTCGATCAC AAGATCGACC AAAACAACTG 
AATTTGCATC TGTTAAATTC AGGAGCAAAA GATGTGTCGA TCATGAGTGT TAGGCCAACT 
CCATCGAACG AAGCTGTCAC AGTTGAGTTC AAGCCAATTA CATTAAAAGC TGGAGAGAGC 
CGATATACCA AAGTTGCAAG TATTAGTTTT GATGCTTCAA AAGCAAGAAG ATCAGACCAG 
TTTTCTGGAA AGATCACAGT GAAGGCAAAA GAGAAAAGCT ACTCAAAACT TGAAATCCCT 
TACCAGGCTG AGGTTTTAGA AGGGTACCTG GGCTTCGACC ACACAGCCAC CCTGTTCCAC 
ATCAGAGACA GTCCTGTAGA TCCAGTGGAC AGACCCATCT ACCTCACCAA TACCTTCAAC 
TTTGCCATAC GCATTCACAA TGTGTCACTT CCCAAAGAAG CCAAGACTAT GTTCAATGTG 
CATAATTTCA GCACTCCAGT ACTCATCCCT CCCCATGAGT CCCGCTACAT TTTCTCTCTT 
CTTTTTCGAC CAGTACGACC CTCCATTCAC ATCGACAGCA ACATCCTCCT CATCACCAAT 
GCTTCTAAAT TTCACCTGCC AGTTCGGGCC TACACAGGCT TCTTGGAGCC GGTTGTTCTT 
CCTCCCTGTC TTCAAGAGCA CATCTTAGAC TTTGGTGTTC TTAGTGCCAC AGACACCAGC 
AGTTTGGTGT TTGTTGTGGT CAACAGCAAT CCAATTGAGT TGGAGATAAA GTCATGGCTG 
GTGACAGGAG ACAGTCTGTC CATGGAATTG CTTAACACAG AAAGAGGGAA CAGAAGTATA 
GCACTAAGCA GAATTCAAGA GCTGCAGAAC ACATCAGCTG CCCAATACAA AACTGTCATT 
TTAGCCTCAG GATATTATGC AGCTTTTAAA GTCACTTTGG TGGCTAAAGC TCTAGAGGGA 
ATTTATGATG GAGCCGTACA CATTACAACA GATTATGAGA TATTGACAAT CCCTGTTAAA 
GCTCTTATAG CTGTAGGAAC ATTAAGCTGT TCACCCAAAC ATATCATTCT TCCTGATGCA 
TTCCCGGGGA AAGTAGTCCA CCAAGGGTTG AGTATACAGA GCTCATTCTC ACAGAAAGTA 
AGGCTTCAGC AGATTCGCTC TCTCACAGAG GACGTCCGGT TTCAGTACAA ACGAGTTCGC 
AATAATAAAG TGGAGTTGGA ACCCAGGCGA AAGTCCAAGA TAGCGAACAT TTATTTTGAT 
GCCAGCTTGC AATGTGGCGA TCACTGTTAT GTGGGATTAC CGTTTGTATT GAAATCGGAG 
TCTAGGCCTC ATGGTTTAGT GATGCAGGAG GACATGTGGG ACGCTGATGT AGATCTCCAC 
CAGCGGCTCC TAAAAAGATG GCGAGAGCTC AAGGAGCAAT CAGGCCATGA GGTCGAAGCC 
TTCTTTGAAG TGGACACAGA TCTGCAGAAA AATGTGCAGG CCAAGATTTC AGCAAAGCTC 
AAATGGCCCT CGCTGCTCAA CTCTACCCTA CAACAGATCC ATTTTCCTCT CACAAACACA 
AACAGCTCAT CTGAGGAAGA AGTGATTCTA GAAAACTCTG CTGATGTCCC AGTCTACGTT 
CAGGTTCTTC CTCTGGCTTT ATACCCCAAT CCCTCAGTGT TCACTGGCAA ACTTGCTGAC 
AGGTTTCCTC TTGGAAAGTT ATCAAACATA AACCTGGACA CAAATACACT GGAATTCCTG 
GTCCAGAAAA ATCATTCTTC AGTGATGAAG AGTTCCACAG GTTTTGTTGA AGGATCGACA 
CGGCCCTATG TATATAACAT GCTCCTTATG CCTGGGGAGG TGAAATCCTT CAATGTAAGG 
TTTAGACCCA CCACCAATCA CAGTGTCTCT TCTCTGCTTG TCGTCAGGAA CAACTTGACG 
ATCGTAGACT TGATCATGGT TCATGGTCAG GGCACCACAG AGAGTCTGAG ATTGGCCGGT 
AAAGCCCCAG GTCTCGGCAG CTCTCTGAGG TTTAAACTTA CCGAGTCATT CCTTAAAGAC 
TGTTCAGAAG TTAAAGCCAA GGAACCAAAC TTCACTCTTC GAAGGACTTT TAGGCTGGAG 
AATACGGGCC TGCTTCCGAT TCACATTCGG TCTGTGGAGA TCAGTGGCAA TGCATGTGAG 
GGTTATGGCT TCAAAGTTCT CAACTGTGAA GAATTTGCAC TCAAACCAAA CACTTCGAAA 
GACCTCATCA TACTGTTTAC TCCAGATTTC ACATCATCCA GAGTGATCCG CGAGCTGAAG 
TTGGTGACTG CTGGGGGCTC AGTGTTTGCC TTTGTGCTGA ATGCCTCATT GCCATATCAC 
ATGCTGGCAG TGTGTGCTGA GATGCTTCCC CGACCCAACT GGGAGCTTGA GCTCTACATC 
ATCGTCTCCC TCGTTATGAG CTCCATGTTC CTGCTGGTCA TCGCTACAGC CTATCTGGAA 
GCCCAGGGAA TATGGGAGCC TTTCAAGCGA CGGCTGTCTG TGGAATCCAA CTCTCCCATG 
GAGACTGGAA AACCATTTGA TCTCAGGGAA ATTGTACGAA TTCAAAGTGA TCCAAACTTG 
AATGATTTTG CTGACTCAAA CCAGAACTCG ACGAGAGGAA TGTACGGGTC AAGCATAGGT 
GGGCGGGCTG GGGGTCGGCA AAGTGGCTCA CACTCTAGTT CCAACAGCAA CGGTGTTTTG 
CCAGATAGCA AGCCCAGATC TGGCTCAGGC CGCTCTGGAT CTATGCAATC CTCTTCCACC 
TTTTCACTCA AAGATAATGG TCCAGGACTC AACCAGGTGA CTAACCGAAA AGCTCGAAGT 
TCCAAACAGC AGCAGCAGGG CTCAGGCCTT CTTGAGGATC TGCCAAGTAC AGGACCCTCA 
CAGTCTAACA GCGCAAGGGT CAACAGTACA GAAGATCAGG ATTATAATCG ACTCCTGGTA 
GCCATGGATA AAGACCTGGA CAGGCCTGAG TCTCCTGCCA TTAATGTGTC TTCAGAGCAC 
AATTCACAAC AGTCTTCACA AAACAAAGTG CTTGAATCAA AAGGGAAGAA TGAAACAAAA 
GTAAAGAATA AAACTAAAGC GCTAAAGAAG AAAGAGGAGA AAGAAAGGAA AACAAAGGGA 
AAGACTCAAG GGGAGGATTT TAAAGAGCCC TTGGCAGACA ATGACGACAG CTCCTCCACT 
ACCACAGAGA CCTCTAACCC TGATGTGGAG GCCAATATTA AAGAGGAGCC AGTGAAGGTG 
AAAGGAAAAA CTGTGGCAAT AGACAATGGA AAAGAAAGAG AAGAAATTAC TCCCTCCCCT 
ACTAAACCAA AAAGTAAAAA ACAACCTACA ACAAAGAAGG AGAACCCTGT GGAAAAATCC 
AGTTCTTTAG AGCTCCCCTA TGTGACACCA CTGGAAAAAC AGGACAAGCG GAAGAACTTC 
AAAACACTTG CCAGTACAGC AATTCCTGGA GTATCCAAAC CACGAGCTTT GAGCAAATTA 
GTCAATGCAA AGATGGGTGA TGACCGGCCA TCTCCACTTG GAAAGTTTGT GTCTAATGGC 
TCCATGCCAG AGTTGGGTCA TAGCAGTGGA TCAGAAGGAG AAAAAGAGTC TCCTCCACCA 
GAATGGGATG TTCCTCTCAA CAAAAATGGC AACCAAGCAG ACAGTCTCCA GCAGATATCT 
ATGCAGACCA TGCATGCAGA CCCCTTCCTG AAAGGACCCA GTTCAGTCAT CAACAGAACC 
TGTTCCCCAC CTCCAACCTC CCCCAGCCTG GTGCCTCATG GAAGCTACAG CAGCATGATG 
CACCCCAGCA GTGAAATTAA TTTAAAGAAG GCTCCAGGGA ATAAGCTCAC CAAGTCAGCC 
TCTCTGCCTG GTCAAAATGG AAATCCAACT TTTGCCGCTG TGGCTGCTGG CTACGATAAA 
AGCCCTGGTG GCAGTGGCTG TGCTAGTAAA GCTGACGGTT TGGGGAAGGT GGCACTGCCT 
CACATGACAT CAGTGGAGAG TGACGGATCT GACAGCTCTG GACTCTGGAG CCCAGTAGAC 
TCCACCAGCA GCCCCAGTTT TAACCAAGTT AATGCTTTTT CTGCTTTTGG ACCAAACAAC 
CCACTCAACC TCTCTGGAGT TTTCAGAGGC ATGACTGTTC CCAAGAGTTC AGAATCCCAG 
CAGAGTTGGC CTGAGTACAC TCCAGTGTCT CCATCCATCT GGGATACAGA GACTGATAAT 
TCCTGGACCA CCATCACTGG ATCCCCAGCC ACTCCAACAA CTTCCATTTT GGGGAGCGCA 
CGCAGTCCCT GGTCTTTCAG CAGTTCCATC TGGTCCACTG GCAACGACTC CACCCTTCAC 
TCCTTCAGTC CTCCTGCAAC ATCCACCCTC CCTGACCTGG TCAGTCACAC TGCACCTCCC 
ACACCTGCGC CAAACGACAT GGGCCGCACC TACAATCCCT GGAGTATGGG CTGGTCTCCC 
TCCCTCAACC GCTGGAACTC TGAGCCCTGG CCAAACAGCC CAGACAACAA CAATGGCATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166018.1
CDS	37..5439
Translation	MASKDTTQSSLHPTRTKSACVGLLRLLFIAFIHVAQANKQAFIQSDNILEVLHFGDGSLLQTESDIDLSSYRQKSASSQRGNSRPIRFEPPMLDFHEQPVGMPKMEKVYLHNPSSEEISLISISATTSHFHASFFQNRIIPPGGNTSFDVVFLARVVGNVENTLFINTSHHGVFTYQVFGVGVPNPYRLRPFVGARVPVNSSFSPLISIFNPHSEPLQVVEMYTSGGDLHLELPTGQQGGTKKLWEIAPFETKGVMRASFSSRDADNHTAFIRIKTNASNEDEFIILPVEVEVTTAPGIYSSTEMLDFGTLRSQDRPKQLNLHLLNSGAKDVSIMSVRPTPSNEAVTVEFKPITLKAGESRYTKVASISFDASKARRSDQFSGKITVKAKEKSYSKLEIPYQAEVLEGYLGFDHTATLFHIRDSPVDPVDRPIYLTNTFNFAIRIHNVSLPKEAKTMFNVHNFSTPVLIPPHESRYIFSLLFRPVRPSIHIDSNILLITNASKFHLPVRAYTGFLEPVVLPPCLQEHILDFGVLSATDTSSLVFVVVNSNPIELEIKSWLVTGDSLSMELLNTERGNRSIALSRIQELQNTSAAQYKTVILASGYYAAFKVTLVAKALEGIYDGAVHITTDYEILTIPVKALIAVGTLSCSPKHIILPDAFPGKVVHQGLSIQSSFSQKVRLQQIRSLTEDVRFQYKRVRNNKVELEPRRKSKIANIYFDASLQCGDHCYVGLPFVLKSESRPHGLVMQEDMWDADVDLHQRLLKRWRELKEQSGHEVEAFFEVDTDLQKNVQAKISAKLKWPSLLNSTLQQIHFPLTNTNSSSEEEVILENSADVPVYVQVLPLALYPNPSVFTGKLADRFPLGKLSNINLDTNTLEFLVQKNHSSVMKSSTGFVEGSTRPYVYNMLLMPGEVKSFNVRFRPTTNHSVSSLLVVRNNLTIVDLIMVHGQGTTESLRLAGKAPGLGSSLRFKLTESFLKDCSEVKAKEPNFTLRRTFRLENTGLLPIHIRSVEISGNACEGYGFKVLNCEEFALKPNTSKDLIILFTPDFTSSRVIRELKLVTAGGSVFAFVLNASLPYHMLAVCAEMLPRPNWELELYIIVSLVMSSMFLLVIATAYLEAQGIWEPFKRRLSVESNSPMETGKPFDLREIVRIQSDPNLNDFADSNQNSTRGMYGSSIGGRAGGRQSGSHSSSNSNGVLPDSKPRSGSGRSGSMQSSSTFSLKDNGPGLNQVTNRKARSSKQQQQGSGLLEDLPSTGPSQSNSARVNSTEDQDYNRLLVAMDKDLDRPESPAINVSSEHNSQQSSQNKVLESKGKNETKVKNKTKALKKKEEKERKTKGKTQGEDFKEPLADNDDSSSTTTETSNPDVEANIKEEPVKVKGKTVAIDNGKEREEITPSPTKPKSKKQPTTKKENPVEKSSSLELPYVTPLEKQDKRKNFKTLASTAIPGVSKPRALSKLVNAKMGDDRPSPLGKFVSNGSMPELGHSSGSEGEKESPPPEWDVPLNKNGNQADSLQQISMQTMHADPFLKGPSSVINRTCSPPPTSPSLVPHGSYSSMMHPSSEINLKKAPGNKLTKSASLPGQNGNPTFAAVAAGYDKSPGGSGCASKADGLGKVALPHMTSVESDGSDSSGLWSPVDSTSSPSFNQVNAFSAFGPNNPLNLSGVFRGMTVPKSSESQQSWPEYTPVSPSIWDTETDNSWTTITGSPATPTTSILGSARSPWSFSSSIWSTGNDSTLHSFSPPATSTLPDLVSHTAPPTPAPNDMGRTYNPWSMGWSPSLNRWNSEPWPNSPDNNNGI

Target ORF information:

RefSeq Version	XM_005165961.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane protein 131 (tmem131), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165961.4

ORF Insert Sequence:

ATGGCGAGCA AGGACACAAC ACAGAGTTCC CTACATCCGA CACGAACAAA ATCAGCATGT 
GTTGGACTTC TGCGCCTTTT ATTTATTGCA TTTATTCACG TGGCACAGGC GAACAAACAA 
GCTTTCATAC AGTCAGACAA CATCCTGGAG GTACTACACT TTGGGGATGG AAGCCTCTTG 
CAGACAGAGT CTGATATTGA CCTTAGCTCA TATCGTCAAA AAAGTGCATC CTCTCAGCGG 
GGGAACAGTA GGCCCATACG CTTTGAGCCT CCAATGCTGG ACTTTCATGA ACAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTTTACTTA CACAATCCAA GTTCAGAAGA AATAAGTTTA 
ATATCAATAT CAGCAACAAC ATCACATTTT CATGCATCCT TTTTTCAGAA TAGGATAATT 
CCACCTGGAG GCAACACCTC ATTTGATGTG GTGTTTCTAG CTAGAGTAGT AGGGAATGTA 
GAAAATACCT TATTTATTAA TACATCGCAT CATGGAGTGT TTACATACCA GGTGTTTGGT 
GTGGGCGTCC CCAATCCCTA CAGACTCAGG CCCTTTGTAG GGGCAAGAGT TCCAGTAAAT 
AGCAGCTTCT CCCCACTCAT AAGCATCTTC AACCCACACA GTGAACCACT GCAGGTTGTA 
GAGATGTACA CCAGTGGAGG AGACCTACAT TTAGAGCTGC CTACTGGACA GCAGGGTGGC 
ACCAAAAAAT TATGGGAGAT CGCTCCTTTT GAGACGAAGG GTGTGATGAG AGCCAGCTTC 
TCCTCTCGAG ACGCTGACAA CCACACAGCT TTCATCAGAA TAAAAACAAA TGCGTCCAAC 
GAGGACGAGT TTATCATCCT CCCTGTGGAG GTTGAAGTCA CCACAGCACC TGGTATATAC 
TCCTCCACAG AGATGCTGGA CTTTGGTACA CTTCGATCAC AAGATCGACC AAAACAACTG 
AATTTGCATC TGTTAAATTC AGGAGCAAAA GATGTGTCGA TCATGAGTGT TAGGCCAACT 
CCATCGAACG AAGCTGTCAC AGTTGAGTTC AAGCCAATTA CATTAAAAGC TGGAGAGAGC 
CGATATACCA AAGTTGCAAG TATTAGTTTT GATGCTTCAA AAGCAAGAAG ATCAGACCAG 
TTTTCTGGAA AGATCACAGT GAAGGCAAAA GAGAAAAGCT ACTCAAAACT TGAAATCCCT 
TACCAGGCTG AGGTTTTAGA AGGGTACCTG GGCTTCGACC ACACAGCCAC CCTGTTCCAC 
ATCAGAGACA GTCCTGTAGA TCCAGTGGAC AGACCCATCT ACCTCACCAA TACCTTCAAC 
TTTGCCATAC GCATTCACAA TGTGTCACTT CCCAAAGAAG CCAAGACTAT GTTCAATGTG 
CATAATTTCA GCACTCCAGT ACTCATCCCT CCCCATGAGT CCCGCTACAT TTTCTCTCTT 
CTTTTTCGAC CAGTACGACC CTCCATTCAC ATCGACAGCA ACATCCTCCT CATCACCAAT 
GCTTCTAAAT TTCACCTGCC AGTTCGGGCC TACACAGGCT TCTTGGAGCC GGTTGTTCTT 
CCTCCCTGTC TTCAAGAGCA CATCTTAGAC TTTGGTGTTC TTAGTGCCAC AGACACCAGC 
AGTTTGGTGT TTGTTGTGGT CAACAGCAAT CCAATTGAGT TGGAGATAAA GTCATGGCTG 
GTGACAGGAG ACAGTCTGTC CATGGAATTG CTTAACACAG AAAGAGGGAA CAGAAGTATA 
GCACTAAGCA GAATTCAAGA GCTGCAGAAC ACATCAGCTG CCCAATACAA AACTGTCATT 
TTAGCCTCAG GATATTATGC AGCTTTTAAA GTCACTTTGG TGGCTAAAGC TCTAGAGGGA 
ATTTATGATG GAGCCGTACA CATTACAACA GATTATGAGA TATTGACAAT CCCTGTTAAA 
GCTCTTATAG CTGTAGGAAC ATTAAGCTGT TCACCCAAAC ATATCATTCT TCCTGATGCA 
TTCCCGGGGA AAGTAGTCCA CCAAGGGTTG AGTATACAGA GCTCATTCTC ACAGAAAGTA 
AGGCTTCAGC AGATTCGCTC TCTCACAGAG GACGTCCGGT TTCAGTACAA ACGAGTTCGC 
AATAATAAAG TGGAGTTGGA ACCCAGGCGA AAGTCCAAGA TAGCGAACAT TTATTTTGAT 
GCCAGCTTGC AATGTGGCGA TCACTGTTAT GTGGGATTAC CGTTTGTATT GAAATCGGAG 
TCTAGGCCTC ATGGTTTAGT GATGCAGGAG GACATGTGGG ACGCTGATGT AGATCTCCAC 
CAGCGGCTCC TAAAAAGATG GCGAGAGCTC AAGGAGCAAT CAGGCCATGA GGTCGAAGCC 
TTCTTTGAAG TGGACACAGA TCTGCAGAAA AATGTGCAGG CCAAGATTTC AGCAAAGCTC 
AAATGGCCCT CGCTGCTCAA CTCTACCCTA CAACAGATCC ATTTTCCTCT CACAAACACA 
AACAGCTCAT CTGAGGAAGA AGTGATTCTA GAAAACTCTG CTGATGTCCC AGTCTACGTT 
CAGGTTCTTC CTCTGGCTTT ATACCCCAAT CCCTCAGTGT TCACTGGCAA ACTTGCTGAC 
AGGTTTCCTC TTGGAAAGTT ATCAAACATA AACCTGGACA CAAATACACT GGAATTCCTG 
GTCCAGAAAA ATCATTCTTC AGTGATGAAG AGTTCCACAG GTTTTGTTGA AGGATCGACA 
CGGCCCTATG TATATAACAT GCTCCTTATG CCTGGGGAGG TGAAATCCTT CAATGTAAGG 
TTTAGACCCA CCACCAATCA CAGTGTCTCT TCTCTGCTTG TCGTCAGGAA CAACTTGACG 
ATCGTAGACT TGATCATGGT TCATGGTCAG GGCACCACAG AGAGTCTGAG ATTGGCCGGT 
AAAGCCCCAG GTCTCGGCAG CTCTCTGAGG TTTAAACTTA CCGAGTCATT CCTTAAAGAC 
TGTTCAGAAG TTAAAGCCAA GGAACCAAAC TTCACTCTTC GAAGGACTTT TAGGCTGGAG 
AATACGGGCC TGCTTCCGAT TCACATTCGG TCTGTGGAGA TCAGTGGCAA TGCATGTGAG 
GGTTATGGCT TCAAAGTTCT CAACTGTGAA GAATTTGCAC TCAAACCAAA CACTTCGAAA 
GACCTCATCA TACTGTTTAC TCCAGATTTC ACATCATCCA GAGTGATCCG CGAGCTGAAG 
TTGGTGACTG CTGGGGGCTC AGTGTTTGCC TTTGTGCTGA ATGCCTCATT GCCATATCAC 
ATGCTGGCAG TGTGTGCTGA GATGCTTCCC CGACCCAACT GGGAGCTTGA GCTCTACATC 
ATCGTCTCCC TCGTTATGAG CTCCATGTTC CTGCTGGTCA TCGCTACAGC CTATCTGGAA 
GCCCAGGGAA TATGGGAGCC TTTCAAGCGA CGGCTGTCTG TGGAATCCAA CTCTCCCATG 
GAGACTGGAA AACCATTTGA TCTCAGGGAA ATTGTACGAA TTCAAAGTGA TCCAAACTTG 
AATGATTTTG CTGACTCAAA CCAGAACTCG ACGAGAGGAA TGTACGGGTC AAGCATAGGT 
GGGCGGGCTG GGGGTCGGCA AAGTGGCTCA CACTCTAGTT CCAACAGCAA CGGTGTTTTG 
CCAGATAGCA AGCCCAGATC TGGCTCAGGC CGCTCTGGAT CTATGCAATC CTCTTCCACC 
TTTTCACTCA AAGATAATGG TCCAGGACTC AACCAGGTGA CTAACCGAAA AGCTCGAAGT 
TCCAAACAGC AGCAGCAGGG CTCAGGCCTT CTTGAGGATC TGCCAAGTAC AGGACCCTCA 
CAGTCTAACA GCGCAAGGGT CAACAGTACA GAAGATCAGG ATTATAATCG ACTCCTGGTA 
GCCATGGATA AAGACCTGGA CAGGCCTGAG TCTCCTGCCA TTAATGTGTC TTCAGAGCAC 
AATTCACAAC AGTCTTCACA AAACAAAGTG CTTGAATCAA AAGGGAAGAA TGAAACAAAA 
GTAAAGAATA AAACTAAAGC GCTAAAGAAG AAAGAGGAGA AAGAAAGGAA AACAAAGGGA 
AAGACTCAAG GGGAGGATTT TAAAGAGCCC TTGGCAGACA ATGACGACAG CTCCTCCACT 
ACCACAGAGA CCTCTAACCC TGATGTGGAG GCCAATATTA AAGAGGAGCC AGTGAAGGTG 
AAAGGAAAAA CTGTGGCAAT AGACAATGGA AAAGAAAGAG AAGAAATTAC TCCCTCCCCT 
ACTAAACCAA AAAGTAAAAA ACAACCTACA ACAAAGAAGG AGAACCCTGT GGAAAAATCC 
AGTTCTTTAG AGCTCCCCTA TGTGACACCA CTGGAAAAAC AGGACAAGCG GAAGAACTTC 
AAAACACTTG CCAGTACAGC AATTCCTGGA GTATCCAAAC CACGAGCTTT GAGCAAATTA 
GTCAATGCAA AGATGGGTGA TGACCGGCCA TCTCCACTTG GAAAGTTTGT GTCTAATGGC 
TCCATGCCAG AGTTGGGTCA TAGCAGTGGA TCAGAAGGAG AAAAAGAGTC TCCTCCACCA 
GAATGGGATG TTCCTCTCAA CAAAAATGGC AACCAAGCAG ACAGTCTCCA GCAGATATCT 
ATGCAGACCA TGCATGCAGA CCCCTTCCTG AAAGGACCCA GTTCAGTCAT CAACAGAACC 
TGTTCCCCAC CTCCAACCTC CCCCAGCCTG GTGCCTCATG GAAGCTACAG CAGCATGATG 
CACCCCAGCA GTGAAATTAA TTTAAAGAAG GCTCCAGGGA ATAAGCTCAC CAAGTCAGCC 
TCTCTGCCTG GTCAAAATGG AAATCCAACT TTTGCCGCTG TGGCTGCTGG CTACGATAAA 
AGCCCTGGTG GCAGTGGCTG TGCTAGTAAA GCTGACGGTT TGGGGAAGGT GGCACTGCCT 
CACATGACAT CAGTGGAGAG TGACGGATCT GACAGCTCTG GACTCTGGAG CCCAGTAGAC 
TCCACCAGCA GCCCCAGTTT TAACCAAGTT AATGCTTTTT CTGCTTTTGG ACCAAACAAC 
CCACTCAACC TCTCTGGAGT TTTCAGAGGC ATGACTGTTC CCAAGAGTTC AGAATCCCAG 
CAGAGTTGGC CTGAGTACAC TCCAGTGTCT CCATCCATCT GGGATACAGA GACTGATAAT 
TCCTGGACCA CCATCACTGG ATCCCCAGCC ACTCCAACAA CTTCCATTTT GGGGAGCGCA 
CGCAGTCCCT GGTCTTTCAG CAGTTCCATC TGGTCCACTG GCAACGACTC CACCCTTCAC 
TCCTTCAGTC CTCCTGCAAC ATCCACCCTC CCTGACCTGG TCAGTCACAC TGCACCTCCC 
ACACCTGCGC CAAACGACAT GGGCCGCACC TACAATCCCT GGAGTATGGG CTGGTCTCCC 
TCCCTCAACC GCTGGAACTC TGAGCCCTGG CCAAACAGCC CAGACAACAA CAATGGCATC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tmem131 ( XM_001923305.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001923340.1 Danio rerio transmembrane protein 131 (tmem131), transcript variant X2, mRNA.

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CloneID	ODa11898
Clone ID Related Accession (Same CDS sequence)	XM_001923305.5 , XM_021477147.1 , XM_001923305.6
Accession Version	XM_001923305.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transmembrane protein 131 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001923305.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCA AGGACACAAC ACAGAGTTCC CTACATCCGA CACGAACAAA ATCAGCATGT 
GTTGGACTTC TGCGCCTTTT ATTTATTGCA TTTATTCACG TGGCACAGGC GAACAAACAA 
GCTTTCATAC AGTCAGACAA CATCCTGGAG GTACTACACT TTGGGGATGG AAGCCTCTTG 
CAGACAGAGT CTGATATTGA CCTTAGCTCA TATCGTCAAA AAAGTGCATC CTCTCAGCGG 
GGGAACAGTA GGCCCATACG CTTTGAGCCT CCAATGCTGG ACTTTCATGA ACAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTTTACTTA CACAATCCAA GTTCAGAAGA AATAAGTTTA 
ATATCAATAT CAGCAACAAC ATCACATTTT CATGCATCCT TTTTTCAGAA TAGGATAATT 
CCACCTGGAG GCAACACCTC ATTTGATGTG GTGTTTCTAG CTAGAGTAGT AGGGAATGTA 
GAAAATACCT TATTTATTAA TACATCGCAT CATGGAGTGT TTACATACCA GGTGTTTGGT 
GTGGGCGTCC CCAATCCCTA CAGACTCAGG CCCTTTGTAG GGGCAAGAGT TCCAGTAAAT 
AGCAGCTTCT CCCCACTCAT AAGCATCTTC AACCCACACA GTGAACCACT GCAGGTTGTA 
GAGATGTACA CCAGTGGAGG AGACCTACAT TTAGAGCTGC CTACTGGACA GCAGGGTGGC 
ACCAAAAAAT TATGGGAGAT CGCTCCTTTT GAGACGAAGG GTGTGATGAG AGCCAGCTTC 
TCCTCTCGAG ACGCTGACAA CCACACAGCT TTCATCAGAA TAAAAACAAA TGCGTCCAAC 
GAGGACGAGT TTATCATCCT CCCTGTGGAG GTTGAAGTCA CCACAGCACC TGGTATATAC 
TCCTCCACAG AGATGCTGGA CTTTGGTACA CTTCGATCAC AAGATCGACC AAAACAACTG 
AATTTGCATC TGTTAAATTC AGGAGCAAAA GATGTGTCGA TCATGAGTGT TAGGCCAACT 
CCATCGAACG AAGCTGTCAC AGTTGAGTTC AAGCCAATTA CATTAAAAGC TGGAGAGAGC 
CGATATACCA AAGTTGCAAG TATTAGTTTT GATGCTTCAA AAGCAAGAAG ATCAGACCAG 
TTTTCTGGAA AGATCACAGT GAAGGCAAAA GAGAAAAGCT ACTCAAAACT TGAAATCCCT 
TACCAGGCTG AGGTTTTAGA AGGGTACCTG GGCTTCGACC ACACAGCCAC CCTGTTCCAC 
ATCAGAGACA GTCCTGTAGA TCCAGTGGAC AGACCCATCT ACCTCACCAA TACCTTCAAC 
TTTGCCATAC GCATTCACAA TGTGTCACTT CCCAAAGAAG CCAAGACTAT GTTCAATGTG 
CATAATTTCA GCACTCCAGT ACTCATCCCT CCCCATGAGT CCCGCTACAT TTTCTCTCTT 
CTTTTTCGAC CAGTACGACC CTCCATTCAC ATCGACAGCA ACATCCTCCT CATCACCAAT 
GCTTCTAAAT TTCACCTGCC AGTTCGGGCC TACACAGGCT TCTTGGAGCC GGTTGTTCTT 
CCTCCCTGTC TTCAAGAGCA CATCTTAGAC TTTGGTGTTC TTAGTGCCAC AGACACCAGC 
AGTTTGGTGT TTGTTGTGGT CAACAGCAAT CCAATTGAGT TGGAGATAAA GTCATGGCTG 
GTGACAGGAG ACAGTCTGTC CATGGAATTG CTTAACACAG AAAGAGGGAA CAGAAGTATA 
GCACTAAGCA GAATTCAAGA GCTGCAGAAC ACATCAGCTG CCCAATACAA AACTGTCATT 
TTAGCCTCAG GATATTATGC AGCTTTTAAA GTCACTTTGG TGGCTAAAGC TCTAGAGGGA 
ATTTATGATG GAGCCGTACA CATTACAACA GATTATGAGA TATTGACAAT CCCTGTTAAA 
GCTCTTATAG CTGTAGGAAC ATTAAGCTGT TCACCCAAAC ATATCATTCT TCCTGATGCA 
TTCCCGGGGA AAGTAGTCCA CCAAGGGTTG AGTATACAGA GCTCATTCTC ACAGAAAGTA 
AGGCTTCAGC AGATTCGCTC TCTCACAGAG GACGTCCGGT TTCAGTACAA ACGAGTTCGC 
AATAATAAAG TGGAGTTGGA ACCCAGGCGA AAGTCCAAGA TAGCGAACAT TTATTTTGAT 
GCCAGCTTGC AATGTGGCGA TCACTGTTAT GTGGGATTAC CGTTTGTATT GAAATCGGAG 
TCTAGGCCTC ATGGTTTAGT GATGCAGGAG GACATGTGGG ACGCTGATGT AGATCTCCAC 
CAGCGGCTCC TAAAAAGATG GCGAGAGCTC AAGGAGCAAT CAGGCCATGA GGTCGAAGCC 
TTCTTTGAAG TGGACACAGA TCTGCAGAAA AATGTGCAGG CCAAGATTTC AGCAAAGCTC 
AAATGGCCCT CGCTGCTCAA CTCTACCCTA CAACAGATCC ATTTTCCTCT CACAAACACA 
AACAGCTCAT CTGAGGAAGA AGTGATTCTA GAAAACTCTG CTGATGTCCC AGTCTACGTT 
CAGGTTCTTC CTCTGGCTTT ATACCCCAAT CCCTCAGTGT TCACTGGCAA ACTTGCTGAC 
AGGTTTCCTC TTGGAAAGTT ATCAAACATA AACCTGGACA CAAATACACT GGAATTCCTG 
GTCCAGAAAA ATCATTCTTC AGTGATGAAG AGTTCCACAG GTTTTGTTGA AGGATCGACA 
CGGCCCTATG TATATAACAT GCTCCTTATG CCTGGGGAGG TGAAATCCTT CAATGTAAGG 
TTTAGACCCA CCACCAATCA CAGTGTCTCT TCTCTGCTTG TCGTCAGGAA CAACTTGACG 
ATCGTAGACT TGATCATGGT TCATGGTCAG GGCACCACAG AGAGTCTGAG ATTGGCCGGT 
AAAGCCCCAG GTCTCGGCAG CTCTCTGAGG TTTAAACTTA CCGAGTCATT CCTTAAAGAC 
TGTTCAGAAG TTAAAGCCAA GGAACCAAAC TTCACTCTTC GAAGGACTTT TAGGCTGGAG 
AATACGGGCC TGCTTCCGAT TCACATTCGG TCTGTGGAGA TCAGTGGCAA TGCATGTGAG 
GGTTATGGCT TCAAAGTTCT CAACTGTGAA GAATTTGCAC TCAAACCAAA CACTTCGAAA 
GACCTCATCA TACTGTTTAC TCCAGATTTC ACATCATCCA GAGTGATCCG CGAGCTGAAG 
TTGGTGACTG CTGGGGGCTC AGTGTTTGCC TTTGTGCTGA ATGCCTCATT GCCATATCAC 
ATGCTGGCAG TGTGTGCTGA GATGCTTCCC CGACCCAACT GGGAGCTTGA GCTCTACATC 
ATCGTCTCCC TCGTTATGAG CTCCATGTTC CTGCTGGTCA TCGCTACAGC CTATCTGGAA 
GCCCAGGGAA TATGGGAGCC TTTCAAGCGA CGGCTGTCTG TGGAATCCAA CTCTCCCATG 
GAGACTGGAA AACCATTTGA TCTCAGGGAA ATTGTACGAA TTCAAAGTGA TCCAAACTTG 
AATGATTTTG CTGACTCAAA CCAGAACTCG ACGAGAGGAA TGTACGGGTC AAGCATAGGT 
GGGCGGGCTG GGGGTCGGCA AAGTGGCTCA CACTCTAGTT CCAACAGCAA CGGTGTTTTG 
CCAGATAGCA AGCCCAGATC TGGCTCAGGC CGCTCTGGAT CTATGCAATC CTCTTCCACC 
TTTTCACTCA AAGATAATGG TCCAGGACTC AACCAGGTGA CTAACCGAAA AGCTCGAAGT 
TCCAAACAGC AGCAGCAGGG CTCAGGCCTT CTTGAGGATC TGCCAAGTAC AGGACCCTCA 
CAGTCTAACA GCGCAAGGGT CAACAGTACA GAAGATCAGG ATTATAATCG ACTCCTGGTA 
GCCATGGATA AAGACCTGGA CAGGCCTGAG TCTCCTGCCA TTAATGTGTC TTCAGAGCAC 
AATTCACAAC AGTCTTCACA AAACAAAGTG CTTGAATCAA AAGGGAAGAA TGAAACAAAA 
GTAAAGAATA AAACTAAAGC GCTAAAGAAG AAAGAGGAGA AAGAAAGGAA AACAAAGGGA 
AAGACTCAAG GGGAGGATTT TAAAGAGCCC TTGGCAGACA ATGACGACAG CTCCTCCACT 
ACCACAGAGA CCTCTAACCC TGATGTGGAG GCCAATATTA AAGAGGAGCC AGTGAAGGTG 
AAAGGAAAAA CTGTGGCAAT AGACAATGGA AAAGAAAGAG AAGAAATTAC TCCCTCCCCT 
ACTAAACCAA AAAGTAAAAA ACAACCTACA ACAAAGAAGG AGAACCCTGT GGAAAAATCC 
AGTTCTTTAG AGCTCCCCTA TGTGACACCA CTGGAAAAAC AGGACAAGCG GAAGAACTTC 
AAAACACTTG CCAGTACAGC AATTCCTGGA GTATCCAAAC CACGAGCTTT GAGCAAATTC 
AATGCAAAGA TGGGTGATGA CCGGCCATCT CCACTTGGAA AGTTTGTGTC TAATGGCTCC 
ATGCCAGAGT TGGGTCATAG CAGTGGATCA GAAGGAGAAA AAGAGTCTCC TCCACCAGAA 
TGGGATGTTC CTCTCAACAA AAATGGCAAC CAAGCAGACA GTCTCCAGCA GATATCTATG 
CAGACCATGC ATGCAGACCC CTTCCTGAAA GGACCCAGTT CAGTCATCAA CAGAACCTGT 
TCCCCACCTC CAACCTCCCC CAGCCTGGTG CCTCATGGAA GCTACAGCAG CATGATGCAC 
CCCAGCAGTG AAATTAATTT AAAGAAGGCT CCAGGGAATA AGCTCACCAA GTCAGCCTCT 
CTGCCTGGTC AAAATGGAAA TCCAACTTTT GCCGCTGTGG CTGCTGGCTA CGATAAAAGC 
CCTGGTGGCA GTGGCTGTGC TAGTAAAGCT GACGGTTTGG GGAAGGTGGC ACTGCCTCAC 
ATGACATCAG TGGAGAGTGA CGGATCTGAC AGCTCTGGAC TCTGGAGCCC AGTAGACTCC 
ACCAGCAGCC CCAGTTTTAA CCAAGTTAAT GCTTTTTCTG CTTTTGGACC AAACAACCCA 
CTCAACCTCT CTGGAGTTTT CAGAGGCATG ACTGTTCCCA AGAGTTCAGA ATCCCAGCAG 
AGTTGGCCTG AGTACACTCC AGTGTCTCCA TCCATCTGGG ATACAGAGAC TGATAATTCC 
TGGACCACCA TCACTGGATC CCCAGCCACT CCAACAACTT CCATTTTGGG GAGCGCACGC 
AGTCCCTGGT CTTTCAGCAG TTCCATCTGG TCCACTGGCA ACGACTCCAC CCTTCACTCC 
TTCAGTCCTC CTGCAACATC CACCCTCCCT GACCTGGTCA GTCACACTGC ACCTCCCACA 
CCTGCGCCAA ACGACATGGG CCGCACCTAC AATCCCTGGA GTATGGGCTG GTCTCCCTCC 
CTCAACCGCT GGAACTCTGA GCCCTGGCCA AACAGCCCAG ACAACAACAA TGGCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001923340.1
CDS	34..5433
Translation	MASKDTTQSSLHPTRTKSACVGLLRLLFIAFIHVAQANKQAFIQSDNILEVLHFGDGSLLQTESDIDLSSYRQKSASSQRGNSRPIRFEPPMLDFHEQPVGMPKMEKVYLHNPSSEEISLISISATTSHFHASFFQNRIIPPGGNTSFDVVFLARVVGNVENTLFINTSHHGVFTYQVFGVGVPNPYRLRPFVGARVPVNSSFSPLISIFNPHSEPLQVVEMYTSGGDLHLELPTGQQGGTKKLWEIAPFETKGVMRASFSSRDADNHTAFIRIKTNASNEDEFIILPVEVEVTTAPGIYSSTEMLDFGTLRSQDRPKQLNLHLLNSGAKDVSIMSVRPTPSNEAVTVEFKPITLKAGESRYTKVASISFDASKARRSDQFSGKITVKAKEKSYSKLEIPYQAEVLEGYLGFDHTATLFHIRDSPVDPVDRPIYLTNTFNFAIRIHNVSLPKEAKTMFNVHNFSTPVLIPPHESRYIFSLLFRPVRPSIHIDSNILLITNASKFHLPVRAYTGFLEPVVLPPCLQEHILDFGVLSATDTSSLVFVVVNSNPIELEIKSWLVTGDSLSMELLNTERGNRSIALSRIQELQNTSAAQYKTVILASGYYAAFKVTLVAKALEGIYDGAVHITTDYEILTIPVKALIAVGTLSCSPKHIILPDAFPGKVVHQGLSIQSSFSQKVRLQQIRSLTEDVRFQYKRVRNNKVELEPRRKSKIANIYFDASLQCGDHCYVGLPFVLKSESRPHGLVMQEDMWDADVDLHQRLLKRWRELKEQSGHEVEAFFEVDTDLQKNVQAKISAKLKWPSLLNSTLQQIHFPLTNTNSSSEEEVILENSADVPVYVQVLPLALYPNPSVFTGKLADRFPLGKLSNINLDTNTLEFLVQKNHSSVMKSSTGFVEGSTRPYVYNMLLMPGEVKSFNVRFRPTTNHSVSSLLVVRNNLTIVDLIMVHGQGTTESLRLAGKAPGLGSSLRFKLTESFLKDCSEVKAKEPNFTLRRTFRLENTGLLPIHIRSVEISGNACEGYGFKVLNCEEFALKPNTSKDLIILFTPDFTSSRVIRELKLVTAGGSVFAFVLNASLPYHMLAVCAEMLPRPNWELELYIIVSLVMSSMFLLVIATAYLEAQGIWEPFKRRLSVESNSPMETGKPFDLREIVRIQSDPNLNDFADSNQNSTRGMYGSSIGGRAGGRQSGSHSSSNSNGVLPDSKPRSGSGRSGSMQSSSTFSLKDNGPGLNQVTNRKARSSKQQQQGSGLLEDLPSTGPSQSNSARVNSTEDQDYNRLLVAMDKDLDRPESPAINVSSEHNSQQSSQNKVLESKGKNETKVKNKTKALKKKEEKERKTKGKTQGEDFKEPLADNDDSSSTTTETSNPDVEANIKEEPVKVKGKTVAIDNGKEREEITPSPTKPKSKKQPTTKKENPVEKSSSLELPYVTPLEKQDKRKNFKTLASTAIPGVSKPRALSKFNAKMGDDRPSPLGKFVSNGSMPELGHSSGSEGEKESPPPEWDVPLNKNGNQADSLQQISMQTMHADPFLKGPSSVINRTCSPPPTSPSLVPHGSYSSMMHPSSEINLKKAPGNKLTKSASLPGQNGNPTFAAVAAGYDKSPGGSGCASKADGLGKVALPHMTSVESDGSDSSGLWSPVDSTSSPSFNQVNAFSAFGPNNPLNLSGVFRGMTVPKSSESQQSWPEYTPVSPSIWDTETDNSWTTITGSPATPTTSILGSARSPWSFSSSIWSTGNDSTLHSFSPPATSTLPDLVSHTAPPTPAPNDMGRTYNPWSMGWSPSLNRWNSEPWPNSPDNNNGI

Target ORF information:

RefSeq Version	XM_001923305.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transmembrane protein 131 (tmem131), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001923305.5

ORF Insert Sequence:

ATGGCGAGCA AGGACACAAC ACAGAGTTCC CTACATCCGA CACGAACAAA ATCAGCATGT 
GTTGGACTTC TGCGCCTTTT ATTTATTGCA TTTATTCACG TGGCACAGGC GAACAAACAA 
GCTTTCATAC AGTCAGACAA CATCCTGGAG GTACTACACT TTGGGGATGG AAGCCTCTTG 
CAGACAGAGT CTGATATTGA CCTTAGCTCA TATCGTCAAA AAAGTGCATC CTCTCAGCGG 
GGGAACAGTA GGCCCATACG CTTTGAGCCT CCAATGCTGG ACTTTCATGA ACAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTTTACTTA CACAATCCAA GTTCAGAAGA AATAAGTTTA 
ATATCAATAT CAGCAACAAC ATCACATTTT CATGCATCCT TTTTTCAGAA TAGGATAATT 
CCACCTGGAG GCAACACCTC ATTTGATGTG GTGTTTCTAG CTAGAGTAGT AGGGAATGTA 
GAAAATACCT TATTTATTAA TACATCGCAT CATGGAGTGT TTACATACCA GGTGTTTGGT 
GTGGGCGTCC CCAATCCCTA CAGACTCAGG CCCTTTGTAG GGGCAAGAGT TCCAGTAAAT 
AGCAGCTTCT CCCCACTCAT AAGCATCTTC AACCCACACA GTGAACCACT GCAGGTTGTA 
GAGATGTACA CCAGTGGAGG AGACCTACAT TTAGAGCTGC CTACTGGACA GCAGGGTGGC 
ACCAAAAAAT TATGGGAGAT CGCTCCTTTT GAGACGAAGG GTGTGATGAG AGCCAGCTTC 
TCCTCTCGAG ACGCTGACAA CCACACAGCT TTCATCAGAA TAAAAACAAA TGCGTCCAAC 
GAGGACGAGT TTATCATCCT CCCTGTGGAG GTTGAAGTCA CCACAGCACC TGGTATATAC 
TCCTCCACAG AGATGCTGGA CTTTGGTACA CTTCGATCAC AAGATCGACC AAAACAACTG 
AATTTGCATC TGTTAAATTC AGGAGCAAAA GATGTGTCGA TCATGAGTGT TAGGCCAACT 
CCATCGAACG AAGCTGTCAC AGTTGAGTTC AAGCCAATTA CATTAAAAGC TGGAGAGAGC 
CGATATACCA AAGTTGCAAG TATTAGTTTT GATGCTTCAA AAGCAAGAAG ATCAGACCAG 
TTTTCTGGAA AGATCACAGT GAAGGCAAAA GAGAAAAGCT ACTCAAAACT TGAAATCCCT 
TACCAGGCTG AGGTTTTAGA AGGGTACCTG GGCTTCGACC ACACAGCCAC CCTGTTCCAC 
ATCAGAGACA GTCCTGTAGA TCCAGTGGAC AGACCCATCT ACCTCACCAA TACCTTCAAC 
TTTGCCATAC GCATTCACAA TGTGTCACTT CCCAAAGAAG CCAAGACTAT GTTCAATGTG 
CATAATTTCA GCACTCCAGT ACTCATCCCT CCCCATGAGT CCCGCTACAT TTTCTCTCTT 
CTTTTTCGAC CAGTACGACC CTCCATTCAC ATCGACAGCA ACATCCTCCT CATCACCAAT 
GCTTCTAAAT TTCACCTGCC AGTTCGGGCC TACACAGGCT TCTTGGAGCC GGTTGTTCTT 
CCTCCCTGTC TTCAAGAGCA CATCTTAGAC TTTGGTGTTC TTAGTGCCAC AGACACCAGC 
AGTTTGGTGT TTGTTGTGGT CAACAGCAAT CCAATTGAGT TGGAGATAAA GTCATGGCTG 
GTGACAGGAG ACAGTCTGTC CATGGAATTG CTTAACACAG AAAGAGGGAA CAGAAGTATA 
GCACTAAGCA GAATTCAAGA GCTGCAGAAC ACATCAGCTG CCCAATACAA AACTGTCATT 
TTAGCCTCAG GATATTATGC AGCTTTTAAA GTCACTTTGG TGGCTAAAGC TCTAGAGGGA 
ATTTATGATG GAGCCGTACA CATTACAACA GATTATGAGA TATTGACAAT CCCTGTTAAA 
GCTCTTATAG CTGTAGGAAC ATTAAGCTGT TCACCCAAAC ATATCATTCT TCCTGATGCA 
TTCCCGGGGA AAGTAGTCCA CCAAGGGTTG AGTATACAGA GCTCATTCTC ACAGAAAGTA 
AGGCTTCAGC AGATTCGCTC TCTCACAGAG GACGTCCGGT TTCAGTACAA ACGAGTTCGC 
AATAATAAAG TGGAGTTGGA ACCCAGGCGA AAGTCCAAGA TAGCGAACAT TTATTTTGAT 
GCCAGCTTGC AATGTGGCGA TCACTGTTAT GTGGGATTAC CGTTTGTATT GAAATCGGAG 
TCTAGGCCTC ATGGTTTAGT GATGCAGGAG GACATGTGGG ACGCTGATGT AGATCTCCAC 
CAGCGGCTCC TAAAAAGATG GCGAGAGCTC AAGGAGCAAT CAGGCCATGA GGTCGAAGCC 
TTCTTTGAAG TGGACACAGA TCTGCAGAAA AATGTGCAGG CCAAGATTTC AGCAAAGCTC 
AAATGGCCCT CGCTGCTCAA CTCTACCCTA CAACAGATCC ATTTTCCTCT CACAAACACA 
AACAGCTCAT CTGAGGAAGA AGTGATTCTA GAAAACTCTG CTGATGTCCC AGTCTACGTT 
CAGGTTCTTC CTCTGGCTTT ATACCCCAAT CCCTCAGTGT TCACTGGCAA ACTTGCTGAC 
AGGTTTCCTC TTGGAAAGTT ATCAAACATA AACCTGGACA CAAATACACT GGAATTCCTG 
GTCCAGAAAA ATCATTCTTC AGTGATGAAG AGTTCCACAG GTTTTGTTGA AGGATCGACA 
CGGCCCTATG TATATAACAT GCTCCTTATG CCTGGGGAGG TGAAATCCTT CAATGTAAGG 
TTTAGACCCA CCACCAATCA CAGTGTCTCT TCTCTGCTTG TCGTCAGGAA CAACTTGACG 
ATCGTAGACT TGATCATGGT TCATGGTCAG GGCACCACAG AGAGTCTGAG ATTGGCCGGT 
AAAGCCCCAG GTCTCGGCAG CTCTCTGAGG TTTAAACTTA CCGAGTCATT CCTTAAAGAC 
TGTTCAGAAG TTAAAGCCAA GGAACCAAAC TTCACTCTTC GAAGGACTTT TAGGCTGGAG 
AATACGGGCC TGCTTCCGAT TCACATTCGG TCTGTGGAGA TCAGTGGCAA TGCATGTGAG 
GGTTATGGCT TCAAAGTTCT CAACTGTGAA GAATTTGCAC TCAAACCAAA CACTTCGAAA 
GACCTCATCA TACTGTTTAC TCCAGATTTC ACATCATCCA GAGTGATCCG CGAGCTGAAG 
TTGGTGACTG CTGGGGGCTC AGTGTTTGCC TTTGTGCTGA ATGCCTCATT GCCATATCAC 
ATGCTGGCAG TGTGTGCTGA GATGCTTCCC CGACCCAACT GGGAGCTTGA GCTCTACATC 
ATCGTCTCCC TCGTTATGAG CTCCATGTTC CTGCTGGTCA TCGCTACAGC CTATCTGGAA 
GCCCAGGGAA TATGGGAGCC TTTCAAGCGA CGGCTGTCTG TGGAATCCAA CTCTCCCATG 
GAGACTGGAA AACCATTTGA TCTCAGGGAA ATTGTACGAA TTCAAAGTGA TCCAAACTTG 
AATGATTTTG CTGACTCAAA CCAGAACTCG ACGAGAGGAA TGTACGGGTC AAGCATAGGT 
GGGCGGGCTG GGGGTCGGCA AAGTGGCTCA CACTCTAGTT CCAACAGCAA CGGTGTTTTG 
CCAGATAGCA AGCCCAGATC TGGCTCAGGC CGCTCTGGAT CTATGCAATC CTCTTCCACC 
TTTTCACTCA AAGATAATGG TCCAGGACTC AACCAGGTGA CTAACCGAAA AGCTCGAAGT 
TCCAAACAGC AGCAGCAGGG CTCAGGCCTT CTTGAGGATC TGCCAAGTAC AGGACCCTCA 
CAGTCTAACA GCGCAAGGGT CAACAGTACA GAAGATCAGG ATTATAATCG ACTCCTGGTA 
GCCATGGATA AAGACCTGGA CAGGCCTGAG TCTCCTGCCA TTAATGTGTC TTCAGAGCAC 
AATTCACAAC AGTCTTCACA AAACAAAGTG CTTGAATCAA AAGGGAAGAA TGAAACAAAA 
GTAAAGAATA AAACTAAAGC GCTAAAGAAG AAAGAGGAGA AAGAAAGGAA AACAAAGGGA 
AAGACTCAAG GGGAGGATTT TAAAGAGCCC TTGGCAGACA ATGACGACAG CTCCTCCACT 
ACCACAGAGA CCTCTAACCC TGATGTGGAG GCCAATATTA AAGAGGAGCC AGTGAAGGTG 
AAAGGAAAAA CTGTGGCAAT AGACAATGGA AAAGAAAGAG AAGAAATTAC TCCCTCCCCT 
ACTAAACCAA AAAGTAAAAA ACAACCTACA ACAAAGAAGG AGAACCCTGT GGAAAAATCC 
AGTTCTTTAG AGCTCCCCTA TGTGACACCA CTGGAAAAAC AGGACAAGCG GAAGAACTTC 
AAAACACTTG CCAGTACAGC AATTCCTGGA GTATCCAAAC CACGAGCTTT GAGCAAATTC 
AATGCAAAGA TGGGTGATGA CCGGCCATCT CCACTTGGAA AGTTTGTGTC TAATGGCTCC 
ATGCCAGAGT TGGGTCATAG CAGTGGATCA GAAGGAGAAA AAGAGTCTCC TCCACCAGAA 
TGGGATGTTC CTCTCAACAA AAATGGCAAC CAAGCAGACA GTCTCCAGCA GATATCTATG 
CAGACCATGC ATGCAGACCC CTTCCTGAAA GGACCCAGTT CAGTCATCAA CAGAACCTGT 
TCCCCACCTC CAACCTCCCC CAGCCTGGTG CCTCATGGAA GCTACAGCAG CATGATGCAC 
CCCAGCAGTG AAATTAATTT AAAGAAGGCT CCAGGGAATA AGCTCACCAA GTCAGCCTCT 
CTGCCTGGTC AAAATGGAAA TCCAACTTTT GCCGCTGTGG CTGCTGGCTA CGATAAAAGC 
CCTGGTGGCA GTGGCTGTGC TAGTAAAGCT GACGGTTTGG GGAAGGTGGC ACTGCCTCAC 
ATGACATCAG TGGAGAGTGA CGGATCTGAC AGCTCTGGAC TCTGGAGCCC AGTAGACTCC 
ACCAGCAGCC CCAGTTTTAA CCAAGTTAAT GCTTTTTCTG CTTTTGGACC AAACAACCCA 
CTCAACCTCT CTGGAGTTTT CAGAGGCATG ACTGTTCCCA AGAGTTCAGA ATCCCAGCAG 
AGTTGGCCTG AGTACACTCC AGTGTCTCCA TCCATCTGGG ATACAGAGAC TGATAATTCC 
TGGACCACCA TCACTGGATC CCCAGCCACT CCAACAACTT CCATTTTGGG GAGCGCACGC 
AGTCCCTGGT CTTTCAGCAG TTCCATCTGG TCCACTGGCA ACGACTCCAC CCTTCACTCC 
TTCAGTCCTC CTGCAACATC CACCCTCCCT GACCTGGTCA GTCACACTGC ACCTCCCACA 
CCTGCGCCAA ACGACATGGG CCGCACCTAC AATCCCTGGA GTATGGGCTG GTCTCCCTCC 
CTCAACCGCT GGAACTCTGA GCCCTGGCCA AACAGCCCAG ACAACAACAA TGGCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tmem131 ( XM_021477147.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332822.1 Danio rerio transmembrane protein 131 (tmem131), transcript variant X2, mRNA.

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CloneID	ODa11898
Clone ID Related Accession (Same CDS sequence)	XM_001923305.5 , XM_021477147.1 , XM_001923305.6
Accession Version	XM_021477147.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transmembrane protein 131 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCA AGGACACAAC ACAGAGTTCC CTACATCCGA CACGAACAAA ATCAGCATGT 
GTTGGACTTC TGCGCCTTTT ATTTATTGCA TTTATTCACG TGGCACAGGC GAACAAACAA 
GCTTTCATAC AGTCAGACAA CATCCTGGAG GTACTACACT TTGGGGATGG AAGCCTCTTG 
CAGACAGAGT CTGATATTGA CCTTAGCTCA TATCGTCAAA AAAGTGCATC CTCTCAGCGG 
GGGAACAGTA GGCCCATACG CTTTGAGCCT CCAATGCTGG ACTTTCATGA ACAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTTTACTTA CACAATCCAA GTTCAGAAGA AATAAGTTTA 
ATATCAATAT CAGCAACAAC ATCACATTTT CATGCATCCT TTTTTCAGAA TAGGATAATT 
CCACCTGGAG GCAACACCTC ATTTGATGTG GTGTTTCTAG CTAGAGTAGT AGGGAATGTA 
GAAAATACCT TATTTATTAA TACATCGCAT CATGGAGTGT TTACATACCA GGTGTTTGGT 
GTGGGCGTCC CCAATCCCTA CAGACTCAGG CCCTTTGTAG GGGCAAGAGT TCCAGTAAAT 
AGCAGCTTCT CCCCACTCAT AAGCATCTTC AACCCACACA GTGAACCACT GCAGGTTGTA 
GAGATGTACA CCAGTGGAGG AGACCTACAT TTAGAGCTGC CTACTGGACA GCAGGGTGGC 
ACCAAAAAAT TATGGGAGAT CGCTCCTTTT GAGACGAAGG GTGTGATGAG AGCCAGCTTC 
TCCTCTCGAG ACGCTGACAA CCACACAGCT TTCATCAGAA TAAAAACAAA TGCGTCCAAC 
GAGGACGAGT TTATCATCCT CCCTGTGGAG GTTGAAGTCA CCACAGCACC TGGTATATAC 
TCCTCCACAG AGATGCTGGA CTTTGGTACA CTTCGATCAC AAGATCGACC AAAACAACTG 
AATTTGCATC TGTTAAATTC AGGAGCAAAA GATGTGTCGA TCATGAGTGT TAGGCCAACT 
CCATCGAACG AAGCTGTCAC AGTTGAGTTC AAGCCAATTA CATTAAAAGC TGGAGAGAGC 
CGATATACCA AAGTTGCAAG TATTAGTTTT GATGCTTCAA AAGCAAGAAG ATCAGACCAG 
TTTTCTGGAA AGATCACAGT GAAGGCAAAA GAGAAAAGCT ACTCAAAACT TGAAATCCCT 
TACCAGGCTG AGGTTTTAGA AGGGTACCTG GGCTTCGACC ACACAGCCAC CCTGTTCCAC 
ATCAGAGACA GTCCTGTAGA TCCAGTGGAC AGACCCATCT ACCTCACCAA TACCTTCAAC 
TTTGCCATAC GCATTCACAA TGTGTCACTT CCCAAAGAAG CCAAGACTAT GTTCAATGTG 
CATAATTTCA GCACTCCAGT ACTCATCCCT CCCCATGAGT CCCGCTACAT TTTCTCTCTT 
CTTTTTCGAC CAGTACGACC CTCCATTCAC ATCGACAGCA ACATCCTCCT CATCACCAAT 
GCTTCTAAAT TTCACCTGCC AGTTCGGGCC TACACAGGCT TCTTGGAGCC GGTTGTTCTT 
CCTCCCTGTC TTCAAGAGCA CATCTTAGAC TTTGGTGTTC TTAGTGCCAC AGACACCAGC 
AGTTTGGTGT TTGTTGTGGT CAACAGCAAT CCAATTGAGT TGGAGATAAA GTCATGGCTG 
GTGACAGGAG ACAGTCTGTC CATGGAATTG CTTAACACAG AAAGAGGGAA CAGAAGTATA 
GCACTAAGCA GAATTCAAGA GCTGCAGAAC ACATCAGCTG CCCAATACAA AACTGTCATT 
TTAGCCTCAG GATATTATGC AGCTTTTAAA GTCACTTTGG TGGCTAAAGC TCTAGAGGGA 
ATTTATGATG GAGCCGTACA CATTACAACA GATTATGAGA TATTGACAAT CCCTGTTAAA 
GCTCTTATAG CTGTAGGAAC ATTAAGCTGT TCACCCAAAC ATATCATTCT TCCTGATGCA 
TTCCCGGGGA AAGTAGTCCA CCAAGGGTTG AGTATACAGA GCTCATTCTC ACAGAAAGTA 
AGGCTTCAGC AGATTCGCTC TCTCACAGAG GACGTCCGGT TTCAGTACAA ACGAGTTCGC 
AATAATAAAG TGGAGTTGGA ACCCAGGCGA AAGTCCAAGA TAGCGAACAT TTATTTTGAT 
GCCAGCTTGC AATGTGGCGA TCACTGTTAT GTGGGATTAC CGTTTGTATT GAAATCGGAG 
TCTAGGCCTC ATGGTTTAGT GATGCAGGAG GACATGTGGG ACGCTGATGT AGATCTCCAC 
CAGCGGCTCC TAAAAAGATG GCGAGAGCTC AAGGAGCAAT CAGGCCATGA GGTCGAAGCC 
TTCTTTGAAG TGGACACAGA TCTGCAGAAA AATGTGCAGG CCAAGATTTC AGCAAAGCTC 
AAATGGCCCT CGCTGCTCAA CTCTACCCTA CAACAGATCC ATTTTCCTCT CACAAACACA 
AACAGCTCAT CTGAGGAAGA AGTGATTCTA GAAAACTCTG CTGATGTCCC AGTCTACGTT 
CAGGTTCTTC CTCTGGCTTT ATACCCCAAT CCCTCAGTGT TCACTGGCAA ACTTGCTGAC 
AGGTTTCCTC TTGGAAAGTT ATCAAACATA AACCTGGACA CAAATACACT GGAATTCCTG 
GTCCAGAAAA ATCATTCTTC AGTGATGAAG AGTTCCACAG GTTTTGTTGA AGGATCGACA 
CGGCCCTATG TATATAACAT GCTCCTTATG CCTGGGGAGG TGAAATCCTT CAATGTAAGG 
TTTAGACCCA CCACCAATCA CAGTGTCTCT TCTCTGCTTG TCGTCAGGAA CAACTTGACG 
ATCGTAGACT TGATCATGGT TCATGGTCAG GGCACCACAG AGAGTCTGAG ATTGGCCGGT 
AAAGCCCCAG GTCTCGGCAG CTCTCTGAGG TTTAAACTTA CCGAGTCATT CCTTAAAGAC 
TGTTCAGAAG TTAAAGCCAA GGAACCAAAC TTCACTCTTC GAAGGACTTT TAGGCTGGAG 
AATACGGGCC TGCTTCCGAT TCACATTCGG TCTGTGGAGA TCAGTGGCAA TGCATGTGAG 
GGTTATGGCT TCAAAGTTCT CAACTGTGAA GAATTTGCAC TCAAACCAAA CACTTCGAAA 
GACCTCATCA TACTGTTTAC TCCAGATTTC ACATCATCCA GAGTGATCCG CGAGCTGAAG 
TTGGTGACTG CTGGGGGCTC AGTGTTTGCC TTTGTGCTGA ATGCCTCATT GCCATATCAC 
ATGCTGGCAG TGTGTGCTGA GATGCTTCCC CGACCCAACT GGGAGCTTGA GCTCTACATC 
ATCGTCTCCC TCGTTATGAG CTCCATGTTC CTGCTGGTCA TCGCTACAGC CTATCTGGAA 
GCCCAGGGAA TATGGGAGCC TTTCAAGCGA CGGCTGTCTG TGGAATCCAA CTCTCCCATG 
GAGACTGGAA AACCATTTGA TCTCAGGGAA ATTGTACGAA TTCAAAGTGA TCCAAACTTG 
AATGATTTTG CTGACTCAAA CCAGAACTCG ACGAGAGGAA TGTACGGGTC AAGCATAGGT 
GGGCGGGCTG GGGGTCGGCA AAGTGGCTCA CACTCTAGTT CCAACAGCAA CGGTGTTTTG 
CCAGATAGCA AGCCCAGATC TGGCTCAGGC CGCTCTGGAT CTATGCAATC CTCTTCCACC 
TTTTCACTCA AAGATAATGG TCCAGGACTC AACCAGGTGA CTAACCGAAA AGCTCGAAGT 
TCCAAACAGC AGCAGCAGGG CTCAGGCCTT CTTGAGGATC TGCCAAGTAC AGGACCCTCA 
CAGTCTAACA GCGCAAGGGT CAACAGTACA GAAGATCAGG ATTATAATCG ACTCCTGGTA 
GCCATGGATA AAGACCTGGA CAGGCCTGAG TCTCCTGCCA TTAATGTGTC TTCAGAGCAC 
AATTCACAAC AGTCTTCACA AAACAAAGTG CTTGAATCAA AAGGGAAGAA TGAAACAAAA 
GTAAAGAATA AAACTAAAGC GCTAAAGAAG AAAGAGGAGA AAGAAAGGAA AACAAAGGGA 
AAGACTCAAG GGGAGGATTT TAAAGAGCCC TTGGCAGACA ATGACGACAG CTCCTCCACT 
ACCACAGAGA CCTCTAACCC TGATGTGGAG GCCAATATTA AAGAGGAGCC AGTGAAGGTG 
AAAGGAAAAA CTGTGGCAAT AGACAATGGA AAAGAAAGAG AAGAAATTAC TCCCTCCCCT 
ACTAAACCAA AAAGTAAAAA ACAACCTACA ACAAAGAAGG AGAACCCTGT GGAAAAATCC 
AGTTCTTTAG AGCTCCCCTA TGTGACACCA CTGGAAAAAC AGGACAAGCG GAAGAACTTC 
AAAACACTTG CCAGTACAGC AATTCCTGGA GTATCCAAAC CACGAGCTTT GAGCAAATTC 
AATGCAAAGA TGGGTGATGA CCGGCCATCT CCACTTGGAA AGTTTGTGTC TAATGGCTCC 
ATGCCAGAGT TGGGTCATAG CAGTGGATCA GAAGGAGAAA AAGAGTCTCC TCCACCAGAA 
TGGGATGTTC CTCTCAACAA AAATGGCAAC CAAGCAGACA GTCTCCAGCA GATATCTATG 
CAGACCATGC ATGCAGACCC CTTCCTGAAA GGACCCAGTT CAGTCATCAA CAGAACCTGT 
TCCCCACCTC CAACCTCCCC CAGCCTGGTG CCTCATGGAA GCTACAGCAG CATGATGCAC 
CCCAGCAGTG AAATTAATTT AAAGAAGGCT CCAGGGAATA AGCTCACCAA GTCAGCCTCT 
CTGCCTGGTC AAAATGGAAA TCCAACTTTT GCCGCTGTGG CTGCTGGCTA CGATAAAAGC 
CCTGGTGGCA GTGGCTGTGC TAGTAAAGCT GACGGTTTGG GGAAGGTGGC ACTGCCTCAC 
ATGACATCAG TGGAGAGTGA CGGATCTGAC AGCTCTGGAC TCTGGAGCCC AGTAGACTCC 
ACCAGCAGCC CCAGTTTTAA CCAAGTTAAT GCTTTTTCTG CTTTTGGACC AAACAACCCA 
CTCAACCTCT CTGGAGTTTT CAGAGGCATG ACTGTTCCCA AGAGTTCAGA ATCCCAGCAG 
AGTTGGCCTG AGTACACTCC AGTGTCTCCA TCCATCTGGG ATACAGAGAC TGATAATTCC 
TGGACCACCA TCACTGGATC CCCAGCCACT CCAACAACTT CCATTTTGGG GAGCGCACGC 
AGTCCCTGGT CTTTCAGCAG TTCCATCTGG TCCACTGGCA ACGACTCCAC CCTTCACTCC 
TTCAGTCCTC CTGCAACATC CACCCTCCCT GACCTGGTCA GTCACACTGC ACCTCCCACA 
CCTGCGCCAA ACGACATGGG CCGCACCTAC AATCCCTGGA GTATGGGCTG GTCTCCCTCC 
CTCAACCGCT GGAACTCTGA GCCCTGGCCA AACAGCCCAG ACAACAACAA TGGCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332822.1
CDS	37..5436
Translation	MASKDTTQSSLHPTRTKSACVGLLRLLFIAFIHVAQANKQAFIQSDNILEVLHFGDGSLLQTESDIDLSSYRQKSASSQRGNSRPIRFEPPMLDFHEQPVGMPKMEKVYLHNPSSEEISLISISATTSHFHASFFQNRIIPPGGNTSFDVVFLARVVGNVENTLFINTSHHGVFTYQVFGVGVPNPYRLRPFVGARVPVNSSFSPLISIFNPHSEPLQVVEMYTSGGDLHLELPTGQQGGTKKLWEIAPFETKGVMRASFSSRDADNHTAFIRIKTNASNEDEFIILPVEVEVTTAPGIYSSTEMLDFGTLRSQDRPKQLNLHLLNSGAKDVSIMSVRPTPSNEAVTVEFKPITLKAGESRYTKVASISFDASKARRSDQFSGKITVKAKEKSYSKLEIPYQAEVLEGYLGFDHTATLFHIRDSPVDPVDRPIYLTNTFNFAIRIHNVSLPKEAKTMFNVHNFSTPVLIPPHESRYIFSLLFRPVRPSIHIDSNILLITNASKFHLPVRAYTGFLEPVVLPPCLQEHILDFGVLSATDTSSLVFVVVNSNPIELEIKSWLVTGDSLSMELLNTERGNRSIALSRIQELQNTSAAQYKTVILASGYYAAFKVTLVAKALEGIYDGAVHITTDYEILTIPVKALIAVGTLSCSPKHIILPDAFPGKVVHQGLSIQSSFSQKVRLQQIRSLTEDVRFQYKRVRNNKVELEPRRKSKIANIYFDASLQCGDHCYVGLPFVLKSESRPHGLVMQEDMWDADVDLHQRLLKRWRELKEQSGHEVEAFFEVDTDLQKNVQAKISAKLKWPSLLNSTLQQIHFPLTNTNSSSEEEVILENSADVPVYVQVLPLALYPNPSVFTGKLADRFPLGKLSNINLDTNTLEFLVQKNHSSVMKSSTGFVEGSTRPYVYNMLLMPGEVKSFNVRFRPTTNHSVSSLLVVRNNLTIVDLIMVHGQGTTESLRLAGKAPGLGSSLRFKLTESFLKDCSEVKAKEPNFTLRRTFRLENTGLLPIHIRSVEISGNACEGYGFKVLNCEEFALKPNTSKDLIILFTPDFTSSRVIRELKLVTAGGSVFAFVLNASLPYHMLAVCAEMLPRPNWELELYIIVSLVMSSMFLLVIATAYLEAQGIWEPFKRRLSVESNSPMETGKPFDLREIVRIQSDPNLNDFADSNQNSTRGMYGSSIGGRAGGRQSGSHSSSNSNGVLPDSKPRSGSGRSGSMQSSSTFSLKDNGPGLNQVTNRKARSSKQQQQGSGLLEDLPSTGPSQSNSARVNSTEDQDYNRLLVAMDKDLDRPESPAINVSSEHNSQQSSQNKVLESKGKNETKVKNKTKALKKKEEKERKTKGKTQGEDFKEPLADNDDSSSTTTETSNPDVEANIKEEPVKVKGKTVAIDNGKEREEITPSPTKPKSKKQPTTKKENPVEKSSSLELPYVTPLEKQDKRKNFKTLASTAIPGVSKPRALSKFNAKMGDDRPSPLGKFVSNGSMPELGHSSGSEGEKESPPPEWDVPLNKNGNQADSLQQISMQTMHADPFLKGPSSVINRTCSPPPTSPSLVPHGSYSSMMHPSSEINLKKAPGNKLTKSASLPGQNGNPTFAAVAAGYDKSPGGSGCASKADGLGKVALPHMTSVESDGSDSSGLWSPVDSTSSPSFNQVNAFSAFGPNNPLNLSGVFRGMTVPKSSESQQSWPEYTPVSPSIWDTETDNSWTTITGSPATPTTSILGSARSPWSFSSSIWSTGNDSTLHSFSPPATSTLPDLVSHTAPPTPAPNDMGRTYNPWSMGWSPSLNRWNSEPWPNSPDNNNGI

Target ORF information:

RefSeq Version	XM_021477147.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane protein 131 (tmem131), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477147.1

ORF Insert Sequence:

ATGGCGAGCA AGGACACAAC ACAGAGTTCC CTACATCCGA CACGAACAAA ATCAGCATGT 
GTTGGACTTC TGCGCCTTTT ATTTATTGCA TTTATTCACG TGGCACAGGC GAACAAACAA 
GCTTTCATAC AGTCAGACAA CATCCTGGAG GTACTACACT TTGGGGATGG AAGCCTCTTG 
CAGACAGAGT CTGATATTGA CCTTAGCTCA TATCGTCAAA AAAGTGCATC CTCTCAGCGG 
GGGAACAGTA GGCCCATACG CTTTGAGCCT CCAATGCTGG ACTTTCATGA ACAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTTTACTTA CACAATCCAA GTTCAGAAGA AATAAGTTTA 
ATATCAATAT CAGCAACAAC ATCACATTTT CATGCATCCT TTTTTCAGAA TAGGATAATT 
CCACCTGGAG GCAACACCTC ATTTGATGTG GTGTTTCTAG CTAGAGTAGT AGGGAATGTA 
GAAAATACCT TATTTATTAA TACATCGCAT CATGGAGTGT TTACATACCA GGTGTTTGGT 
GTGGGCGTCC CCAATCCCTA CAGACTCAGG CCCTTTGTAG GGGCAAGAGT TCCAGTAAAT 
AGCAGCTTCT CCCCACTCAT AAGCATCTTC AACCCACACA GTGAACCACT GCAGGTTGTA 
GAGATGTACA CCAGTGGAGG AGACCTACAT TTAGAGCTGC CTACTGGACA GCAGGGTGGC 
ACCAAAAAAT TATGGGAGAT CGCTCCTTTT GAGACGAAGG GTGTGATGAG AGCCAGCTTC 
TCCTCTCGAG ACGCTGACAA CCACACAGCT TTCATCAGAA TAAAAACAAA TGCGTCCAAC 
GAGGACGAGT TTATCATCCT CCCTGTGGAG GTTGAAGTCA CCACAGCACC TGGTATATAC 
TCCTCCACAG AGATGCTGGA CTTTGGTACA CTTCGATCAC AAGATCGACC AAAACAACTG 
AATTTGCATC TGTTAAATTC AGGAGCAAAA GATGTGTCGA TCATGAGTGT TAGGCCAACT 
CCATCGAACG AAGCTGTCAC AGTTGAGTTC AAGCCAATTA CATTAAAAGC TGGAGAGAGC 
CGATATACCA AAGTTGCAAG TATTAGTTTT GATGCTTCAA AAGCAAGAAG ATCAGACCAG 
TTTTCTGGAA AGATCACAGT GAAGGCAAAA GAGAAAAGCT ACTCAAAACT TGAAATCCCT 
TACCAGGCTG AGGTTTTAGA AGGGTACCTG GGCTTCGACC ACACAGCCAC CCTGTTCCAC 
ATCAGAGACA GTCCTGTAGA TCCAGTGGAC AGACCCATCT ACCTCACCAA TACCTTCAAC 
TTTGCCATAC GCATTCACAA TGTGTCACTT CCCAAAGAAG CCAAGACTAT GTTCAATGTG 
CATAATTTCA GCACTCCAGT ACTCATCCCT CCCCATGAGT CCCGCTACAT TTTCTCTCTT 
CTTTTTCGAC CAGTACGACC CTCCATTCAC ATCGACAGCA ACATCCTCCT CATCACCAAT 
GCTTCTAAAT TTCACCTGCC AGTTCGGGCC TACACAGGCT TCTTGGAGCC GGTTGTTCTT 
CCTCCCTGTC TTCAAGAGCA CATCTTAGAC TTTGGTGTTC TTAGTGCCAC AGACACCAGC 
AGTTTGGTGT TTGTTGTGGT CAACAGCAAT CCAATTGAGT TGGAGATAAA GTCATGGCTG 
GTGACAGGAG ACAGTCTGTC CATGGAATTG CTTAACACAG AAAGAGGGAA CAGAAGTATA 
GCACTAAGCA GAATTCAAGA GCTGCAGAAC ACATCAGCTG CCCAATACAA AACTGTCATT 
TTAGCCTCAG GATATTATGC AGCTTTTAAA GTCACTTTGG TGGCTAAAGC TCTAGAGGGA 
ATTTATGATG GAGCCGTACA CATTACAACA GATTATGAGA TATTGACAAT CCCTGTTAAA 
GCTCTTATAG CTGTAGGAAC ATTAAGCTGT TCACCCAAAC ATATCATTCT TCCTGATGCA 
TTCCCGGGGA AAGTAGTCCA CCAAGGGTTG AGTATACAGA GCTCATTCTC ACAGAAAGTA 
AGGCTTCAGC AGATTCGCTC TCTCACAGAG GACGTCCGGT TTCAGTACAA ACGAGTTCGC 
AATAATAAAG TGGAGTTGGA ACCCAGGCGA AAGTCCAAGA TAGCGAACAT TTATTTTGAT 
GCCAGCTTGC AATGTGGCGA TCACTGTTAT GTGGGATTAC CGTTTGTATT GAAATCGGAG 
TCTAGGCCTC ATGGTTTAGT GATGCAGGAG GACATGTGGG ACGCTGATGT AGATCTCCAC 
CAGCGGCTCC TAAAAAGATG GCGAGAGCTC AAGGAGCAAT CAGGCCATGA GGTCGAAGCC 
TTCTTTGAAG TGGACACAGA TCTGCAGAAA AATGTGCAGG CCAAGATTTC AGCAAAGCTC 
AAATGGCCCT CGCTGCTCAA CTCTACCCTA CAACAGATCC ATTTTCCTCT CACAAACACA 
AACAGCTCAT CTGAGGAAGA AGTGATTCTA GAAAACTCTG CTGATGTCCC AGTCTACGTT 
CAGGTTCTTC CTCTGGCTTT ATACCCCAAT CCCTCAGTGT TCACTGGCAA ACTTGCTGAC 
AGGTTTCCTC TTGGAAAGTT ATCAAACATA AACCTGGACA CAAATACACT GGAATTCCTG 
GTCCAGAAAA ATCATTCTTC AGTGATGAAG AGTTCCACAG GTTTTGTTGA AGGATCGACA 
CGGCCCTATG TATATAACAT GCTCCTTATG CCTGGGGAGG TGAAATCCTT CAATGTAAGG 
TTTAGACCCA CCACCAATCA CAGTGTCTCT TCTCTGCTTG TCGTCAGGAA CAACTTGACG 
ATCGTAGACT TGATCATGGT TCATGGTCAG GGCACCACAG AGAGTCTGAG ATTGGCCGGT 
AAAGCCCCAG GTCTCGGCAG CTCTCTGAGG TTTAAACTTA CCGAGTCATT CCTTAAAGAC 
TGTTCAGAAG TTAAAGCCAA GGAACCAAAC TTCACTCTTC GAAGGACTTT TAGGCTGGAG 
AATACGGGCC TGCTTCCGAT TCACATTCGG TCTGTGGAGA TCAGTGGCAA TGCATGTGAG 
GGTTATGGCT TCAAAGTTCT CAACTGTGAA GAATTTGCAC TCAAACCAAA CACTTCGAAA 
GACCTCATCA TACTGTTTAC TCCAGATTTC ACATCATCCA GAGTGATCCG CGAGCTGAAG 
TTGGTGACTG CTGGGGGCTC AGTGTTTGCC TTTGTGCTGA ATGCCTCATT GCCATATCAC 
ATGCTGGCAG TGTGTGCTGA GATGCTTCCC CGACCCAACT GGGAGCTTGA GCTCTACATC 
ATCGTCTCCC TCGTTATGAG CTCCATGTTC CTGCTGGTCA TCGCTACAGC CTATCTGGAA 
GCCCAGGGAA TATGGGAGCC TTTCAAGCGA CGGCTGTCTG TGGAATCCAA CTCTCCCATG 
GAGACTGGAA AACCATTTGA TCTCAGGGAA ATTGTACGAA TTCAAAGTGA TCCAAACTTG 
AATGATTTTG CTGACTCAAA CCAGAACTCG ACGAGAGGAA TGTACGGGTC AAGCATAGGT 
GGGCGGGCTG GGGGTCGGCA AAGTGGCTCA CACTCTAGTT CCAACAGCAA CGGTGTTTTG 
CCAGATAGCA AGCCCAGATC TGGCTCAGGC CGCTCTGGAT CTATGCAATC CTCTTCCACC 
TTTTCACTCA AAGATAATGG TCCAGGACTC AACCAGGTGA CTAACCGAAA AGCTCGAAGT 
TCCAAACAGC AGCAGCAGGG CTCAGGCCTT CTTGAGGATC TGCCAAGTAC AGGACCCTCA 
CAGTCTAACA GCGCAAGGGT CAACAGTACA GAAGATCAGG ATTATAATCG ACTCCTGGTA 
GCCATGGATA AAGACCTGGA CAGGCCTGAG TCTCCTGCCA TTAATGTGTC TTCAGAGCAC 
AATTCACAAC AGTCTTCACA AAACAAAGTG CTTGAATCAA AAGGGAAGAA TGAAACAAAA 
GTAAAGAATA AAACTAAAGC GCTAAAGAAG AAAGAGGAGA AAGAAAGGAA AACAAAGGGA 
AAGACTCAAG GGGAGGATTT TAAAGAGCCC TTGGCAGACA ATGACGACAG CTCCTCCACT 
ACCACAGAGA CCTCTAACCC TGATGTGGAG GCCAATATTA AAGAGGAGCC AGTGAAGGTG 
AAAGGAAAAA CTGTGGCAAT AGACAATGGA AAAGAAAGAG AAGAAATTAC TCCCTCCCCT 
ACTAAACCAA AAAGTAAAAA ACAACCTACA ACAAAGAAGG AGAACCCTGT GGAAAAATCC 
AGTTCTTTAG AGCTCCCCTA TGTGACACCA CTGGAAAAAC AGGACAAGCG GAAGAACTTC 
AAAACACTTG CCAGTACAGC AATTCCTGGA GTATCCAAAC CACGAGCTTT GAGCAAATTC 
AATGCAAAGA TGGGTGATGA CCGGCCATCT CCACTTGGAA AGTTTGTGTC TAATGGCTCC 
ATGCCAGAGT TGGGTCATAG CAGTGGATCA GAAGGAGAAA AAGAGTCTCC TCCACCAGAA 
TGGGATGTTC CTCTCAACAA AAATGGCAAC CAAGCAGACA GTCTCCAGCA GATATCTATG 
CAGACCATGC ATGCAGACCC CTTCCTGAAA GGACCCAGTT CAGTCATCAA CAGAACCTGT 
TCCCCACCTC CAACCTCCCC CAGCCTGGTG CCTCATGGAA GCTACAGCAG CATGATGCAC 
CCCAGCAGTG AAATTAATTT AAAGAAGGCT CCAGGGAATA AGCTCACCAA GTCAGCCTCT 
CTGCCTGGTC AAAATGGAAA TCCAACTTTT GCCGCTGTGG CTGCTGGCTA CGATAAAAGC 
CCTGGTGGCA GTGGCTGTGC TAGTAAAGCT GACGGTTTGG GGAAGGTGGC ACTGCCTCAC 
ATGACATCAG TGGAGAGTGA CGGATCTGAC AGCTCTGGAC TCTGGAGCCC AGTAGACTCC 
ACCAGCAGCC CCAGTTTTAA CCAAGTTAAT GCTTTTTCTG CTTTTGGACC AAACAACCCA 
CTCAACCTCT CTGGAGTTTT CAGAGGCATG ACTGTTCCCA AGAGTTCAGA ATCCCAGCAG 
AGTTGGCCTG AGTACACTCC AGTGTCTCCA TCCATCTGGG ATACAGAGAC TGATAATTCC 
TGGACCACCA TCACTGGATC CCCAGCCACT CCAACAACTT CCATTTTGGG GAGCGCACGC 
AGTCCCTGGT CTTTCAGCAG TTCCATCTGG TCCACTGGCA ACGACTCCAC CCTTCACTCC 
TTCAGTCCTC CTGCAACATC CACCCTCCCT GACCTGGTCA GTCACACTGC ACCTCCCACA 
CCTGCGCCAA ACGACATGGG CCGCACCTAC AATCCCTGGA GTATGGGCTG GTCTCCCTCC 
CTCAACCGCT GGAACTCTGA GCCCTGGCCA AACAGCCCAG ACAACAACAA TGGCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tmem131 ( XM_001923305.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001923340.1 Danio rerio transmembrane protein 131 (tmem131), transcript variant X2, mRNA.

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CloneID	ODa11898
Clone ID Related Accession (Same CDS sequence)	XM_001923305.5 , XM_021477147.1 , XM_001923305.6
Accession Version	XM_001923305.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transmembrane protein 131 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394647.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001923305.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCA AGGACACAAC ACAGAGTTCC CTACATCCGA CACGAACAAA ATCAGCATGT 
GTTGGACTTC TGCGCCTTTT ATTTATTGCA TTTATTCACG TGGCACAGGC GAACAAACAA 
GCTTTCATAC AGTCAGACAA CATCCTGGAG GTACTACACT TTGGGGATGG AAGCCTCTTG 
CAGACAGAGT CTGATATTGA CCTTAGCTCA TATCGTCAAA AAAGTGCATC CTCTCAGCGG 
GGGAACAGTA GGCCCATACG CTTTGAGCCT CCAATGCTGG ACTTTCATGA ACAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTTTACTTA CACAATCCAA GTTCAGAAGA AATAAGTTTA 
ATATCAATAT CAGCAACAAC ATCACATTTT CATGCATCCT TTTTTCAGAA TAGGATAATT 
CCACCTGGAG GCAACACCTC ATTTGATGTG GTGTTTCTAG CTAGAGTAGT AGGGAATGTA 
GAAAATACCT TATTTATTAA TACATCGCAT CATGGAGTGT TTACATACCA GGTGTTTGGT 
GTGGGCGTCC CCAATCCCTA CAGACTCAGG CCCTTTGTAG GGGCAAGAGT TCCAGTAAAT 
AGCAGCTTCT CCCCACTCAT AAGCATCTTC AACCCACACA GTGAACCACT GCAGGTTGTA 
GAGATGTACA CCAGTGGAGG AGACCTACAT TTAGAGCTGC CTACTGGACA GCAGGGTGGC 
ACCAAAAAAT TATGGGAGAT CGCTCCTTTT GAGACGAAGG GTGTGATGAG AGCCAGCTTC 
TCCTCTCGAG ACGCTGACAA CCACACAGCT TTCATCAGAA TAAAAACAAA TGCGTCCAAC 
GAGGACGAGT TTATCATCCT CCCTGTGGAG GTTGAAGTCA CCACAGCACC TGGTATATAC 
TCCTCCACAG AGATGCTGGA CTTTGGTACA CTTCGATCAC AAGATCGACC AAAACAACTG 
AATTTGCATC TGTTAAATTC AGGAGCAAAA GATGTGTCGA TCATGAGTGT TAGGCCAACT 
CCATCGAACG AAGCTGTCAC AGTTGAGTTC AAGCCAATTA CATTAAAAGC TGGAGAGAGC 
CGATATACCA AAGTTGCAAG TATTAGTTTT GATGCTTCAA AAGCAAGAAG ATCAGACCAG 
TTTTCTGGAA AGATCACAGT GAAGGCAAAA GAGAAAAGCT ACTCAAAACT TGAAATCCCT 
TACCAGGCTG AGGTTTTAGA AGGGTACCTG GGCTTCGACC ACACAGCCAC CCTGTTCCAC 
ATCAGAGACA GTCCTGTAGA TCCAGTGGAC AGACCCATCT ACCTCACCAA TACCTTCAAC 
TTTGCCATAC GCATTCACAA TGTGTCACTT CCCAAAGAAG CCAAGACTAT GTTCAATGTG 
CATAATTTCA GCACTCCAGT ACTCATCCCT CCCCATGAGT CCCGCTACAT TTTCTCTCTT 
CTTTTTCGAC CAGTACGACC CTCCATTCAC ATCGACAGCA ACATCCTCCT CATCACCAAT 
GCTTCTAAAT TTCACCTGCC AGTTCGGGCC TACACAGGCT TCTTGGAGCC GGTTGTTCTT 
CCTCCCTGTC TTCAAGAGCA CATCTTAGAC TTTGGTGTTC TTAGTGCCAC AGACACCAGC 
AGTTTGGTGT TTGTTGTGGT CAACAGCAAT CCAATTGAGT TGGAGATAAA GTCATGGCTG 
GTGACAGGAG ACAGTCTGTC CATGGAATTG CTTAACACAG AAAGAGGGAA CAGAAGTATA 
GCACTAAGCA GAATTCAAGA GCTGCAGAAC ACATCAGCTG CCCAATACAA AACTGTCATT 
TTAGCCTCAG GATATTATGC AGCTTTTAAA GTCACTTTGG TGGCTAAAGC TCTAGAGGGA 
ATTTATGATG GAGCCGTACA CATTACAACA GATTATGAGA TATTGACAAT CCCTGTTAAA 
GCTCTTATAG CTGTAGGAAC ATTAAGCTGT TCACCCAAAC ATATCATTCT TCCTGATGCA 
TTCCCGGGGA AAGTAGTCCA CCAAGGGTTG AGTATACAGA GCTCATTCTC ACAGAAAGTA 
AGGCTTCAGC AGATTCGCTC TCTCACAGAG GACGTCCGGT TTCAGTACAA ACGAGTTCGC 
AATAATAAAG TGGAGTTGGA ACCCAGGCGA AAGTCCAAGA TAGCGAACAT TTATTTTGAT 
GCCAGCTTGC AATGTGGCGA TCACTGTTAT GTGGGATTAC CGTTTGTATT GAAATCGGAG 
TCTAGGCCTC ATGGTTTAGT GATGCAGGAG GACATGTGGG ACGCTGATGT AGATCTCCAC 
CAGCGGCTCC TAAAAAGATG GCGAGAGCTC AAGGAGCAAT CAGGCCATGA GGTCGAAGCC 
TTCTTTGAAG TGGACACAGA TCTGCAGAAA AATGTGCAGG CCAAGATTTC AGCAAAGCTC 
AAATGGCCCT CGCTGCTCAA CTCTACCCTA CAACAGATCC ATTTTCCTCT CACAAACACA 
AACAGCTCAT CTGAGGAAGA AGTGATTCTA GAAAACTCTG CTGATGTCCC AGTCTACGTT 
CAGGTTCTTC CTCTGGCTTT ATACCCCAAT CCCTCAGTGT TCACTGGCAA ACTTGCTGAC 
AGGTTTCCTC TTGGAAAGTT ATCAAACATA AACCTGGACA CAAATACACT GGAATTCCTG 
GTCCAGAAAA ATCATTCTTC AGTGATGAAG AGTTCCACAG GTTTTGTTGA AGGATCGACA 
CGGCCCTATG TATATAACAT GCTCCTTATG CCTGGGGAGG TGAAATCCTT CAATGTAAGG 
TTTAGACCCA CCACCAATCA CAGTGTCTCT TCTCTGCTTG TCGTCAGGAA CAACTTGACG 
ATCGTAGACT TGATCATGGT TCATGGTCAG GGCACCACAG AGAGTCTGAG ATTGGCCGGT 
AAAGCCCCAG GTCTCGGCAG CTCTCTGAGG TTTAAACTTA CCGAGTCATT CCTTAAAGAC 
TGTTCAGAAG TTAAAGCCAA GGAACCAAAC TTCACTCTTC GAAGGACTTT TAGGCTGGAG 
AATACGGGCC TGCTTCCGAT TCACATTCGG TCTGTGGAGA TCAGTGGCAA TGCATGTGAG 
GGTTATGGCT TCAAAGTTCT CAACTGTGAA GAATTTGCAC TCAAACCAAA CACTTCGAAA 
GACCTCATCA TACTGTTTAC TCCAGATTTC ACATCATCCA GAGTGATCCG CGAGCTGAAG 
TTGGTGACTG CTGGGGGCTC AGTGTTTGCC TTTGTGCTGA ATGCCTCATT GCCATATCAC 
ATGCTGGCAG TGTGTGCTGA GATGCTTCCC CGACCCAACT GGGAGCTTGA GCTCTACATC 
ATCGTCTCCC TCGTTATGAG CTCCATGTTC CTGCTGGTCA TCGCTACAGC CTATCTGGAA 
GCCCAGGGAA TATGGGAGCC TTTCAAGCGA CGGCTGTCTG TGGAATCCAA CTCTCCCATG 
GAGACTGGAA AACCATTTGA TCTCAGGGAA ATTGTACGAA TTCAAAGTGA TCCAAACTTG 
AATGATTTTG CTGACTCAAA CCAGAACTCG ACGAGAGGAA TGTACGGGTC AAGCATAGGT 
GGGCGGGCTG GGGGTCGGCA AAGTGGCTCA CACTCTAGTT CCAACAGCAA CGGTGTTTTG 
CCAGATAGCA AGCCCAGATC TGGCTCAGGC CGCTCTGGAT CTATGCAATC CTCTTCCACC 
TTTTCACTCA AAGATAATGG TCCAGGACTC AACCAGGTGA CTAACCGAAA AGCTCGAAGT 
TCCAAACAGC AGCAGCAGGG CTCAGGCCTT CTTGAGGATC TGCCAAGTAC AGGACCCTCA 
CAGTCTAACA GCGCAAGGGT CAACAGTACA GAAGATCAGG ATTATAATCG ACTCCTGGTA 
GCCATGGATA AAGACCTGGA CAGGCCTGAG TCTCCTGCCA TTAATGTGTC TTCAGAGCAC 
AATTCACAAC AGTCTTCACA AAACAAAGTG CTTGAATCAA AAGGGAAGAA TGAAACAAAA 
GTAAAGAATA AAACTAAAGC GCTAAAGAAG AAAGAGGAGA AAGAAAGGAA AACAAAGGGA 
AAGACTCAAG GGGAGGATTT TAAAGAGCCC TTGGCAGACA ATGACGACAG CTCCTCCACT 
ACCACAGAGA CCTCTAACCC TGATGTGGAG GCCAATATTA AAGAGGAGCC AGTGAAGGTG 
AAAGGAAAAA CTGTGGCAAT AGACAATGGA AAAGAAAGAG AAGAAATTAC TCCCTCCCCT 
ACTAAACCAA AAAGTAAAAA ACAACCTACA ACAAAGAAGG AGAACCCTGT GGAAAAATCC 
AGTTCTTTAG AGCTCCCCTA TGTGACACCA CTGGAAAAAC AGGACAAGCG GAAGAACTTC 
AAAACACTTG CCAGTACAGC AATTCCTGGA GTATCCAAAC CACGAGCTTT GAGCAAATTC 
AATGCAAAGA TGGGTGATGA CCGGCCATCT CCACTTGGAA AGTTTGTGTC TAATGGCTCC 
ATGCCAGAGT TGGGTCATAG CAGTGGATCA GAAGGAGAAA AAGAGTCTCC TCCACCAGAA 
TGGGATGTTC CTCTCAACAA AAATGGCAAC CAAGCAGACA GTCTCCAGCA GATATCTATG 
CAGACCATGC ATGCAGACCC CTTCCTGAAA GGACCCAGTT CAGTCATCAA CAGAACCTGT 
TCCCCACCTC CAACCTCCCC CAGCCTGGTG CCTCATGGAA GCTACAGCAG CATGATGCAC 
CCCAGCAGTG AAATTAATTT AAAGAAGGCT CCAGGGAATA AGCTCACCAA GTCAGCCTCT 
CTGCCTGGTC AAAATGGAAA TCCAACTTTT GCCGCTGTGG CTGCTGGCTA CGATAAAAGC 
CCTGGTGGCA GTGGCTGTGC TAGTAAAGCT GACGGTTTGG GGAAGGTGGC ACTGCCTCAC 
ATGACATCAG TGGAGAGTGA CGGATCTGAC AGCTCTGGAC TCTGGAGCCC AGTAGACTCC 
ACCAGCAGCC CCAGTTTTAA CCAAGTTAAT GCTTTTTCTG CTTTTGGACC AAACAACCCA 
CTCAACCTCT CTGGAGTTTT CAGAGGCATG ACTGTTCCCA AGAGTTCAGA ATCCCAGCAG 
AGTTGGCCTG AGTACACTCC AGTGTCTCCA TCCATCTGGG ATACAGAGAC TGATAATTCC 
TGGACCACCA TCACTGGATC CCCAGCCACT CCAACAACTT CCATTTTGGG GAGCGCACGC 
AGTCCCTGGT CTTTCAGCAG TTCCATCTGG TCCACTGGCA ACGACTCCAC CCTTCACTCC 
TTCAGTCCTC CTGCAACATC CACCCTCCCT GACCTGGTCA GTCACACTGC ACCTCCCACA 
CCTGCGCCAA ACGACATGGG CCGCACCTAC AATCCCTGGA GTATGGGCTG GTCTCCCTCC 
CTCAACCGCT GGAACTCTGA GCCCTGGCCA AACAGCCCAG ACAACAACAA TGGCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001923340.1
CDS	37..5436
Translation	MASKDTTQSSLHPTRTKSACVGLLRLLFIAFIHVAQANKQAFIQSDNILEVLHFGDGSLLQTESDIDLSSYRQKSASSQRGNSRPIRFEPPMLDFHEQPVGMPKMEKVYLHNPSSEEISLISISATTSHFHASFFQNRIIPPGGNTSFDVVFLARVVGNVENTLFINTSHHGVFTYQVFGVGVPNPYRLRPFVGARVPVNSSFSPLISIFNPHSEPLQVVEMYTSGGDLHLELPTGQQGGTKKLWEIAPFETKGVMRASFSSRDADNHTAFIRIKTNASNEDEFIILPVEVEVTTAPGIYSSTEMLDFGTLRSQDRPKQLNLHLLNSGAKDVSIMSVRPTPSNEAVTVEFKPITLKAGESRYTKVASISFDASKARRSDQFSGKITVKAKEKSYSKLEIPYQAEVLEGYLGFDHTATLFHIRDSPVDPVDRPIYLTNTFNFAIRIHNVSLPKEAKTMFNVHNFSTPVLIPPHESRYIFSLLFRPVRPSIHIDSNILLITNASKFHLPVRAYTGFLEPVVLPPCLQEHILDFGVLSATDTSSLVFVVVNSNPIELEIKSWLVTGDSLSMELLNTERGNRSIALSRIQELQNTSAAQYKTVILASGYYAAFKVTLVAKALEGIYDGAVHITTDYEILTIPVKALIAVGTLSCSPKHIILPDAFPGKVVHQGLSIQSSFSQKVRLQQIRSLTEDVRFQYKRVRNNKVELEPRRKSKIANIYFDASLQCGDHCYVGLPFVLKSESRPHGLVMQEDMWDADVDLHQRLLKRWRELKEQSGHEVEAFFEVDTDLQKNVQAKISAKLKWPSLLNSTLQQIHFPLTNTNSSSEEEVILENSADVPVYVQVLPLALYPNPSVFTGKLADRFPLGKLSNINLDTNTLEFLVQKNHSSVMKSSTGFVEGSTRPYVYNMLLMPGEVKSFNVRFRPTTNHSVSSLLVVRNNLTIVDLIMVHGQGTTESLRLAGKAPGLGSSLRFKLTESFLKDCSEVKAKEPNFTLRRTFRLENTGLLPIHIRSVEISGNACEGYGFKVLNCEEFALKPNTSKDLIILFTPDFTSSRVIRELKLVTAGGSVFAFVLNASLPYHMLAVCAEMLPRPNWELELYIIVSLVMSSMFLLVIATAYLEAQGIWEPFKRRLSVESNSPMETGKPFDLREIVRIQSDPNLNDFADSNQNSTRGMYGSSIGGRAGGRQSGSHSSSNSNGVLPDSKPRSGSGRSGSMQSSSTFSLKDNGPGLNQVTNRKARSSKQQQQGSGLLEDLPSTGPSQSNSARVNSTEDQDYNRLLVAMDKDLDRPESPAINVSSEHNSQQSSQNKVLESKGKNETKVKNKTKALKKKEEKERKTKGKTQGEDFKEPLADNDDSSSTTTETSNPDVEANIKEEPVKVKGKTVAIDNGKEREEITPSPTKPKSKKQPTTKKENPVEKSSSLELPYVTPLEKQDKRKNFKTLASTAIPGVSKPRALSKFNAKMGDDRPSPLGKFVSNGSMPELGHSSGSEGEKESPPPEWDVPLNKNGNQADSLQQISMQTMHADPFLKGPSSVINRTCSPPPTSPSLVPHGSYSSMMHPSSEINLKKAPGNKLTKSASLPGQNGNPTFAAVAAGYDKSPGGSGCASKADGLGKVALPHMTSVESDGSDSSGLWSPVDSTSSPSFNQVNAFSAFGPNNPLNLSGVFRGMTVPKSSESQQSWPEYTPVSPSIWDTETDNSWTTITGSPATPTTSILGSARSPWSFSSSIWSTGNDSTLHSFSPPATSTLPDLVSHTAPPTPAPNDMGRTYNPWSMGWSPSLNRWNSEPWPNSPDNNNGI

Target ORF information:

RefSeq Version	XM_001923305.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane protein 131 (tmem131), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001923305.6

ORF Insert Sequence:

ATGGCGAGCA AGGACACAAC ACAGAGTTCC CTACATCCGA CACGAACAAA ATCAGCATGT 
GTTGGACTTC TGCGCCTTTT ATTTATTGCA TTTATTCACG TGGCACAGGC GAACAAACAA 
GCTTTCATAC AGTCAGACAA CATCCTGGAG GTACTACACT TTGGGGATGG AAGCCTCTTG 
CAGACAGAGT CTGATATTGA CCTTAGCTCA TATCGTCAAA AAAGTGCATC CTCTCAGCGG 
GGGAACAGTA GGCCCATACG CTTTGAGCCT CCAATGCTGG ACTTTCATGA ACAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTTTACTTA CACAATCCAA GTTCAGAAGA AATAAGTTTA 
ATATCAATAT CAGCAACAAC ATCACATTTT CATGCATCCT TTTTTCAGAA TAGGATAATT 
CCACCTGGAG GCAACACCTC ATTTGATGTG GTGTTTCTAG CTAGAGTAGT AGGGAATGTA 
GAAAATACCT TATTTATTAA TACATCGCAT CATGGAGTGT TTACATACCA GGTGTTTGGT 
GTGGGCGTCC CCAATCCCTA CAGACTCAGG CCCTTTGTAG GGGCAAGAGT TCCAGTAAAT 
AGCAGCTTCT CCCCACTCAT AAGCATCTTC AACCCACACA GTGAACCACT GCAGGTTGTA 
GAGATGTACA CCAGTGGAGG AGACCTACAT TTAGAGCTGC CTACTGGACA GCAGGGTGGC 
ACCAAAAAAT TATGGGAGAT CGCTCCTTTT GAGACGAAGG GTGTGATGAG AGCCAGCTTC 
TCCTCTCGAG ACGCTGACAA CCACACAGCT TTCATCAGAA TAAAAACAAA TGCGTCCAAC 
GAGGACGAGT TTATCATCCT CCCTGTGGAG GTTGAAGTCA CCACAGCACC TGGTATATAC 
TCCTCCACAG AGATGCTGGA CTTTGGTACA CTTCGATCAC AAGATCGACC AAAACAACTG 
AATTTGCATC TGTTAAATTC AGGAGCAAAA GATGTGTCGA TCATGAGTGT TAGGCCAACT 
CCATCGAACG AAGCTGTCAC AGTTGAGTTC AAGCCAATTA CATTAAAAGC TGGAGAGAGC 
CGATATACCA AAGTTGCAAG TATTAGTTTT GATGCTTCAA AAGCAAGAAG ATCAGACCAG 
TTTTCTGGAA AGATCACAGT GAAGGCAAAA GAGAAAAGCT ACTCAAAACT TGAAATCCCT 
TACCAGGCTG AGGTTTTAGA AGGGTACCTG GGCTTCGACC ACACAGCCAC CCTGTTCCAC 
ATCAGAGACA GTCCTGTAGA TCCAGTGGAC AGACCCATCT ACCTCACCAA TACCTTCAAC 
TTTGCCATAC GCATTCACAA TGTGTCACTT CCCAAAGAAG CCAAGACTAT GTTCAATGTG 
CATAATTTCA GCACTCCAGT ACTCATCCCT CCCCATGAGT CCCGCTACAT TTTCTCTCTT 
CTTTTTCGAC CAGTACGACC CTCCATTCAC ATCGACAGCA ACATCCTCCT CATCACCAAT 
GCTTCTAAAT TTCACCTGCC AGTTCGGGCC TACACAGGCT TCTTGGAGCC GGTTGTTCTT 
CCTCCCTGTC TTCAAGAGCA CATCTTAGAC TTTGGTGTTC TTAGTGCCAC AGACACCAGC 
AGTTTGGTGT TTGTTGTGGT CAACAGCAAT CCAATTGAGT TGGAGATAAA GTCATGGCTG 
GTGACAGGAG ACAGTCTGTC CATGGAATTG CTTAACACAG AAAGAGGGAA CAGAAGTATA 
GCACTAAGCA GAATTCAAGA GCTGCAGAAC ACATCAGCTG CCCAATACAA AACTGTCATT 
TTAGCCTCAG GATATTATGC AGCTTTTAAA GTCACTTTGG TGGCTAAAGC TCTAGAGGGA 
ATTTATGATG GAGCCGTACA CATTACAACA GATTATGAGA TATTGACAAT CCCTGTTAAA 
GCTCTTATAG CTGTAGGAAC ATTAAGCTGT TCACCCAAAC ATATCATTCT TCCTGATGCA 
TTCCCGGGGA AAGTAGTCCA CCAAGGGTTG AGTATACAGA GCTCATTCTC ACAGAAAGTA 
AGGCTTCAGC AGATTCGCTC TCTCACAGAG GACGTCCGGT TTCAGTACAA ACGAGTTCGC 
AATAATAAAG TGGAGTTGGA ACCCAGGCGA AAGTCCAAGA TAGCGAACAT TTATTTTGAT 
GCCAGCTTGC AATGTGGCGA TCACTGTTAT GTGGGATTAC CGTTTGTATT GAAATCGGAG 
TCTAGGCCTC ATGGTTTAGT GATGCAGGAG GACATGTGGG ACGCTGATGT AGATCTCCAC 
CAGCGGCTCC TAAAAAGATG GCGAGAGCTC AAGGAGCAAT CAGGCCATGA GGTCGAAGCC 
TTCTTTGAAG TGGACACAGA TCTGCAGAAA AATGTGCAGG CCAAGATTTC AGCAAAGCTC 
AAATGGCCCT CGCTGCTCAA CTCTACCCTA CAACAGATCC ATTTTCCTCT CACAAACACA 
AACAGCTCAT CTGAGGAAGA AGTGATTCTA GAAAACTCTG CTGATGTCCC AGTCTACGTT 
CAGGTTCTTC CTCTGGCTTT ATACCCCAAT CCCTCAGTGT TCACTGGCAA ACTTGCTGAC 
AGGTTTCCTC TTGGAAAGTT ATCAAACATA AACCTGGACA CAAATACACT GGAATTCCTG 
GTCCAGAAAA ATCATTCTTC AGTGATGAAG AGTTCCACAG GTTTTGTTGA AGGATCGACA 
CGGCCCTATG TATATAACAT GCTCCTTATG CCTGGGGAGG TGAAATCCTT CAATGTAAGG 
TTTAGACCCA CCACCAATCA CAGTGTCTCT TCTCTGCTTG TCGTCAGGAA CAACTTGACG 
ATCGTAGACT TGATCATGGT TCATGGTCAG GGCACCACAG AGAGTCTGAG ATTGGCCGGT 
AAAGCCCCAG GTCTCGGCAG CTCTCTGAGG TTTAAACTTA CCGAGTCATT CCTTAAAGAC 
TGTTCAGAAG TTAAAGCCAA GGAACCAAAC TTCACTCTTC GAAGGACTTT TAGGCTGGAG 
AATACGGGCC TGCTTCCGAT TCACATTCGG TCTGTGGAGA TCAGTGGCAA TGCATGTGAG 
GGTTATGGCT TCAAAGTTCT CAACTGTGAA GAATTTGCAC TCAAACCAAA CACTTCGAAA 
GACCTCATCA TACTGTTTAC TCCAGATTTC ACATCATCCA GAGTGATCCG CGAGCTGAAG 
TTGGTGACTG CTGGGGGCTC AGTGTTTGCC TTTGTGCTGA ATGCCTCATT GCCATATCAC 
ATGCTGGCAG TGTGTGCTGA GATGCTTCCC CGACCCAACT GGGAGCTTGA GCTCTACATC 
ATCGTCTCCC TCGTTATGAG CTCCATGTTC CTGCTGGTCA TCGCTACAGC CTATCTGGAA 
GCCCAGGGAA TATGGGAGCC TTTCAAGCGA CGGCTGTCTG TGGAATCCAA CTCTCCCATG 
GAGACTGGAA AACCATTTGA TCTCAGGGAA ATTGTACGAA TTCAAAGTGA TCCAAACTTG 
AATGATTTTG CTGACTCAAA CCAGAACTCG ACGAGAGGAA TGTACGGGTC AAGCATAGGT 
GGGCGGGCTG GGGGTCGGCA AAGTGGCTCA CACTCTAGTT CCAACAGCAA CGGTGTTTTG 
CCAGATAGCA AGCCCAGATC TGGCTCAGGC CGCTCTGGAT CTATGCAATC CTCTTCCACC 
TTTTCACTCA AAGATAATGG TCCAGGACTC AACCAGGTGA CTAACCGAAA AGCTCGAAGT 
TCCAAACAGC AGCAGCAGGG CTCAGGCCTT CTTGAGGATC TGCCAAGTAC AGGACCCTCA 
CAGTCTAACA GCGCAAGGGT CAACAGTACA GAAGATCAGG ATTATAATCG ACTCCTGGTA 
GCCATGGATA AAGACCTGGA CAGGCCTGAG TCTCCTGCCA TTAATGTGTC TTCAGAGCAC 
AATTCACAAC AGTCTTCACA AAACAAAGTG CTTGAATCAA AAGGGAAGAA TGAAACAAAA 
GTAAAGAATA AAACTAAAGC GCTAAAGAAG AAAGAGGAGA AAGAAAGGAA AACAAAGGGA 
AAGACTCAAG GGGAGGATTT TAAAGAGCCC TTGGCAGACA ATGACGACAG CTCCTCCACT 
ACCACAGAGA CCTCTAACCC TGATGTGGAG GCCAATATTA AAGAGGAGCC AGTGAAGGTG 
AAAGGAAAAA CTGTGGCAAT AGACAATGGA AAAGAAAGAG AAGAAATTAC TCCCTCCCCT 
ACTAAACCAA AAAGTAAAAA ACAACCTACA ACAAAGAAGG AGAACCCTGT GGAAAAATCC 
AGTTCTTTAG AGCTCCCCTA TGTGACACCA CTGGAAAAAC AGGACAAGCG GAAGAACTTC 
AAAACACTTG CCAGTACAGC AATTCCTGGA GTATCCAAAC CACGAGCTTT GAGCAAATTC 
AATGCAAAGA TGGGTGATGA CCGGCCATCT CCACTTGGAA AGTTTGTGTC TAATGGCTCC 
ATGCCAGAGT TGGGTCATAG CAGTGGATCA GAAGGAGAAA AAGAGTCTCC TCCACCAGAA 
TGGGATGTTC CTCTCAACAA AAATGGCAAC CAAGCAGACA GTCTCCAGCA GATATCTATG 
CAGACCATGC ATGCAGACCC CTTCCTGAAA GGACCCAGTT CAGTCATCAA CAGAACCTGT 
TCCCCACCTC CAACCTCCCC CAGCCTGGTG CCTCATGGAA GCTACAGCAG CATGATGCAC 
CCCAGCAGTG AAATTAATTT AAAGAAGGCT CCAGGGAATA AGCTCACCAA GTCAGCCTCT 
CTGCCTGGTC AAAATGGAAA TCCAACTTTT GCCGCTGTGG CTGCTGGCTA CGATAAAAGC 
CCTGGTGGCA GTGGCTGTGC TAGTAAAGCT GACGGTTTGG GGAAGGTGGC ACTGCCTCAC 
ATGACATCAG TGGAGAGTGA CGGATCTGAC AGCTCTGGAC TCTGGAGCCC AGTAGACTCC 
ACCAGCAGCC CCAGTTTTAA CCAAGTTAAT GCTTTTTCTG CTTTTGGACC AAACAACCCA 
CTCAACCTCT CTGGAGTTTT CAGAGGCATG ACTGTTCCCA AGAGTTCAGA ATCCCAGCAG 
AGTTGGCCTG AGTACACTCC AGTGTCTCCA TCCATCTGGG ATACAGAGAC TGATAATTCC 
TGGACCACCA TCACTGGATC CCCAGCCACT CCAACAACTT CCATTTTGGG GAGCGCACGC 
AGTCCCTGGT CTTTCAGCAG TTCCATCTGG TCCACTGGCA ACGACTCCAC CCTTCACTCC 
TTCAGTCCTC CTGCAACATC CACCCTCCCT GACCTGGTCA GTCACACTGC ACCTCCCACA 
CCTGCGCCAA ACGACATGGG CCGCACCTAC AATCCCTGGA GTATGGGCTG GTCTCCCTCC 
CTCAACCGCT GGAACTCTGA GCCCTGGCCA AACAGCCCAG ACAACAACAA TGGCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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tmem131 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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