Tmem131 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tmem131
Entrez Gene ID	56030
Full Name	transmembrane protein 131
Synonyms	2610524E03Rik,CC28,D1Bwg0491e,Neg,RW1,YR-23,mKIAA0257
General protein information	Preferred Names transmembrane protein 131 Names transmembrane protein 131
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 B\|1 15.42 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006496152.2	XP_006496215.1	transmembrane protein 131 isoform X4
XM_017321814.2	XP_017177303.1	transmembrane protein 131 isoform X2
XM_006496152.3	XP_006496215.1	transmembrane protein 131 isoform X3
XM_006496150.2	XP_006496213.1	transmembrane protein 131 isoform X1
XM_011238561.2	XP_011236863.1	transmembrane protein 131 isoform X3
XM_006496150.3	XP_006496213.1	transmembrane protein 131 isoform X1
NM_018872.2	NP_061360.2	transmembrane protein 131
XM_017321814.1	XP_017177303.1	transmembrane protein 131 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TMEM131	XP_515638.2
	TMEM131	XP_002808093.1
Danio rerio (zebrafish)	tmem131	XP_005166018.1
Xenopus tropicalis (tropical clawed frog)	tmem131	NP_001135552.1
Bos taurus (cattle)	TMEM131	XP_002691187.1
Homo sapiens (human)	TMEM131	NP_056163.1
Canis lupus familiaris (dog)	TMEM131	XP_531794.3
Rattus norvegicus (Norway rat)	Tmem131	XP_237056.4
Gallus gallus (chicken)	TMEM131	NP_001012712.2
Mus musculus (house mouse)	Tmem131	NP_061360.2

Gene Ontology
Bibliography

Related articles in PubMed

Tagging genes with cassette-exchange sites.
Cobellis G, Nicolaus G, Iovino M, Romito A, Marra E, Barbarisi M, Sardiello M, Di Giorgio FP, Iovino N, Zollo M, Ballabio A, Cortese R
Nucleic acids research33(4)e44(2005 Mar)

Neg, a nerve growth factor-stimulated gene expressed by fetal neocortical neurons that is downregulated by ethanol.
Miller MW, Jacobs JS, Yokoyama R
The Journal of comparative neurology460(2)212-22(2003 May)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Hara Y, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes9(5)179-88(2002 Oct)

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Piao Y, Ko NT, Lim MK, Ko MS
Genome research11(9)1553-8(2001 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tmem131 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tmem131 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu27534	XM_006496150.3 Latest version!	Mus musculus transmembrane protein 131 (Tmem131), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu27536	XM_006496152.3 Latest version!	Mus musculus transmembrane protein 131 (Tmem131), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu77244	XM_017321814.2 Latest version!	Mus musculus transmembrane protein 131 (Tmem131), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu27535	XM_011238561.2 Latest version!	Mus musculus transmembrane protein 131 (Tmem131), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu20765	NM_018872.2 Latest version!	Mus musculus transmembrane protein 131 (Tmem131), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1378.30 $1969.00
View Old Accession Versions >>
OMu27536	XM_006496152.2	Mus musculus transmembrane protein 131 (Tmem131), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu27534	XM_006496150.2	Mus musculus transmembrane protein 131 (Tmem131), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu77244	XM_017321814.1	Mus musculus transmembrane protein 131 (Tmem131), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tmem131 ( XM_006496150.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496213.1 Mus musculus transmembrane protein 131 (Tmem131), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu27534
Clone ID Related Accession (Same CDS sequence)	XM_006496150.2 , XM_006496150.3
Accession Version	XM_006496150.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	transmembrane protein 131 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496150.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGC GGGCGGGAGG AGCAGCCGCC GCCGCCGCCG CCGCCTCCAC GTCCTCCGCG 
GCCGGGCTGG AGCCCGCGGC TGGCCGAGGC GGGGGTCCTC GGAGCGCAGC CGCGGGCCTC 
CTGGGAGCGC TGCACCTGGT GATGACCCTC GTGGTGGCCG CGGCGCGGGC CGAGAAGGAA 
GCATTCATTC AGTCGGAGAG CATAATAGAA GTCCTGCGCT TTGACGACGG AGGGCTACTA 
CAGACTGAGA CAACACTTGG CCTCGGTTCA TATCAACAGA AAAGCATTTC TCTGTACCGG 
GGTAATTGCA GGCCCATACG ATTCGAACCG CCCATGCTGG ATTTCCATGA GCAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTCTACTTA CATAACCCTA GTTCTGAAGA AACGATTACT 
TTAGTATCAA TATCTGCTAC CACATCACAT TTCCATGCAT CGTTTTTTCA AAATAGGAAA 
ATTCTTCCAG GAGGAAACAC ATCATTTGAT GTAGTTTTTC TTGCAAGAGT AGTAGGAAAT 
GTAGAAAATA CTTTATTTAT TAACACATCT AACCATGGGG TGTTCACTTA CCAGGTATTT 
GGTGTTGGGG TTCCAAATCC GTATCGATTA AGGCCATTTC TTGGGGCCAG AGTCCCCGTA 
AACAGCAGCT TTTCACCCAT AATAAACATA CACAATCCTC ACAGTGAGCC TTTACAGGTC 
GTAGAGATGT ACTCGAGTGG AGGTGACCTT CACCTAGAGC TCCCAACAGG TCAGCAAGGA 
GGCACCAGGA AGCTGTGGGA AATCCCTCCT TATGAAACCA AGGGAGTGAT GAGAGCCAGC 
TTTTCCTCCA GAGAAGCAGA CAATCACACA GCCTTCATCC GGATAAAGAC GAATGCATCA 
GACAGCACCG AGTTCATCAT TCTCCCGGTT GAGGTTGAAG TTACAACAGC TCCTGGAATC 
TATTCCTCAA CTGAAATGTT AGATTTTGGA ACACTTAGAA CACAAGATCT ACCAAAGGTT 
TTAAATCTTC ATTTACTAAA TTCAGGAACA AAGGATGTAC CAATAACAAG TGTTCGACCA 
ACACCACAAA ATGACGCTAT CACGGTACAT TTTAAACCAG TTACGTTAAA AGCTTCTGAA 
AGTAAATATA CCAAGGTTGC CAGCATTAGT TTTGATGCTT CGAGAGCAAA AAAGCCATCT 
CAATTTTCTG GGAAAATAAC TGTTAAAGCA AAGGAAAAGA GTTATTCTAA ACTCGAAATC 
CCCTACCAAG CAGAAGTTCT AGATGGTTAT TTGGGGTTTG ATCACGCCGC CACGCTGTTT 
CACATCCAGG ACAGTCCTGC TGACCCAGTG GAGAGGCCGA TCTACCTGAC GAACACTTTC 
AGTTTTGCGA TCCTCATTCA TGATGTGCTG TTACCAGAAG AAGCCAGAAT AATGTTTCAG 
GTGCACAACT TCAGCCAGCC GGTTCTCATT CTTCCTAATG AGTCGGGATA CATTTTTACC 
CTGTTTTTTA TGCCATCCAC CTCATCCATG CACATAGACA ACAACATTTT ACTTGTTACC 
AATGCTTCTA AGTTTCATTT ACCTGTGCGG GTATACACAG GCTTTTTAGA TTACTTTGTA 
TTGCCCCCCA AAATAGAAGA ACGCTTCATA GATTTTGGAG TACTGAGTGC TACAGAAGCA 
AGTAGTATTT TATTTGCAAT TATAAACAGC AATCCAATTG AGCTGGCAAT AAAAAGTTGG 
CATATCATAG GAGATGGCTT ATCAATAGAA CTTGTAGCTA CAGAGAGAGG CAACAGAAGC 
ACGGTGATTG CAAGCCTTCC AGAACTGGAG CGGTCCTCTT TGCCCGACCA GTCCCCGGTA 
ACATTAGCTT CAGGACACTT TGCAGTGTTC AGAGTCAAAC TTACTGCCAA GAAGCTAGAA 
GGGGTCCATG ATGGGGCCAT ACAGATCACA ACCGACTACG AGATCCTGAC CATCCCTGTG 
AAAGCTGTGA TCGCCGTGGG CTCGCTGACC TGCTTCCCCA AGCACATGGT TCTTCCCCCT 
TCCTTCCCAG GGAAAATAGT TCATCAGAGC TTAAATATTA TGAATTCCTT CTCACAGAAG 
GTGAAGATAC AGCAAATAAG GTCTTTGTCA GAAGACGTAC GATTCTACTA CAAACGATTA 
CGGGGTAATA GGGAAGACTT GGAGCCAGGG AAGAAGTCTA AGATTGCAAA CATTTATTTT 
GACCCTGGGC TGCAGTGTGG GGATCACTGC TATATCGGCT TGCCTTTCCT ATCCAAATCT 
GAACCCAAAG TACAGCCTGG GGTGGCCATG CAGGAGGACT TGTGGGATGC CGACTGGGAC 
GCACATCAGA GCTTGTTCAA GGCTTGGATG GGGATAAAGG AAAACGCAGG ACATAGGTTG 
AATGCTATGT TTGAAGTAAA TACAGATCTT CAGAAAAATA TCGTATCAAA AGTCAGTGCC 
GAGCTCTCCT GGCCGTCTGT CCTTAGCTCG CCCCGGCTCC TGAAGTTCCC ACTCACTAAC 
ACAAACTGTT CTTCGGAAGA AGAGATTAGC TTAGAAAACC CTGCAGACGT CCCTGTCTAC 
GTCCAGTTCA TCCCACTGGC TTTGTATTCC AACCCCTCCG TCTTTGCAGA TAAGTTAGTC 
TCAAGGTTTA ACTTGAGTAA GGTGGCCAAG CTAGATTTGA GAACACTTGA ATTCCAAGTC 
TACAGGAACA GCGCTCATCC ACTGCAAAGT CCAACAGGAT TTACAGAGGG TCTCTCTCGG 
CATTTCATTT TAAACCTCAT TTTAAAACCT GGAGAAAAGA AATCTGTCAA AGTAAAGTTC 
ACTCCACTCC ACAACAGAAC TGTGTCTTCC CTGATCATAG TCAGAAACAA CCTGACTGTG 
ATGGACGCTG TGATGGTTCA AGGTCAAGGG ACAACGGAGA ACTTGAGGGT GGCAGGCAAG 
CTTCCAGGTC CAGGAAGCTC CTTACGCTTC AAAATCACAG AAGCATTGTT AAAGGATTGC 
ATAGACCGTT TGAAACTAAG AGAACCAAAT TTCACATTGA AAAGAACATT CAAAGTAGAG 
AATACGGGAC AACTTGAAAT CCGTGTAGAA ACCATTGAGA TCAGTGGATA TGCGTGTGAG 
GGATATGGTT TTAAAGTGGT TAACTGTCAA GAGTTTGCCC TGAGTGCCAA CGCCTCCAGA 
GACATAGTCA TATTGTTTAC TCCAGATTTC ACAGCCTCCA GAGTCATTCG GGAGCTGAAG 
TTTGTGACAA GCAGTGGCTC CGAGTTTGTG TTTGTGTTGA ATGCCTCTCT TCCGTACCAC 
ATGCTAGCCG CCTGTGCAGA AGCCCTCCCT AGACCCAACT GGGAGCTCGC GCTCTACATC 
ATCATCTCCG GGGTCATGAG TGCACTCTTT CTCCTGGTCA TTGGAACAGC CTACTTGGAA 
GCTCAAGGGA TTTGGGAGCC CTTCCGAAGG CGACTCTCCT TTGAAGCCTC AAACCCGCCC 
TTTGATGTAG GAAGGCCATT TGATCTCAGG AGAATCGTTG GTATTTCATC TGAAGGAAAC 
TTGAACACAC TTGGCTGCGA ACACAGTCAT GGTAGGGGTT TCTATAGTAA CGCATCATCC 
CGGCCTGGTA CAGGCAGTCA CAGGCAGTGT GGCACATCAG TCCACCCACA CAGCAGCCAT 
GGCAGCAAAA ACTCAGCTGA TGTGGACAAC GTCAGAACCA GAAACAGTTC AAGTATGTCA 
AGCAGGACTT CTCCACAAGC AGCTGCCTCA CAGTCTACAA GCAAAACCAG CCCCCTAGTC 
TCAGAGACAG CTGCAGCAAC CCAGGGTCAC ACAGCAAGCA GGAAGTCCAG AGGGGCAAAG 
CAGGGCCAGC ACAGCAGCCA GCACCACAGC CACAGCCACA GCCCACTGGA GCAGCACTCA 
CAGCCTCCAC CACCAGTGCC TCAGCACCAG GAGCCACCAC CCGAAAGGCT GTCTCCTGCC 
CCCCTCACAC ACCCTTCCCA CCCCGAGCGA GCCAGCACCA CACGGCACAG CTCAGAGGAC 
TCCGACATCA CCAGTCTCAT AGAAGCCATG GACAAAGACT TTGACCACCA CGACTCCTCC 
CCCCTAGACG TGTTTACAGA GCAGCCTCCG TCACCGATGT CAAAAAGCAA AGGGAAAGGA 
AAGTCTCTCC AACAACGTAA GGCCAAGCCT CCGAAGAAGC AGGAGGAGAA GGAGAAGAGG 
GGGAAGGGGA AGCCGCAGGA GGATGAGCTG AAGGACGCTC TGGCCGACGA CGACAGCTCC 
TCCACCACCA CAGAGACCTC CAACCCCGAC ACAGAGCCAC TCCTCAGGGA GGATACAGAG 
AAGCACAAGG GGAGACCAGC CGTGCCTGAA AAACAGGAAA GTGAACTGTC TCAAGGGAAG 
CCCAAGAGCA AAAAGCTCTT AAACGCTAAG AAAGAAATCC CAACAGATGT GAAAGGCAGT 
TCCTTTGAAC TACCGTACAC TCCCTCACTG GAAAATAAAC AGCGTAGAAA TCTCCCAACC 
AAGATTCCTC TTCCAACTAC ACTAGCAAGT GGATCCAAAT CACGAAATCC CCCGAAAACA 
AAAGGTACAA ATAAGTTAGT GGAAAACAGG CCAGTTGCCC TCTCAAAGTT CCTCCCCAGC 
AGTCAAGAAC TAGGTAATAC CAGCAGCTCA GAGGGTGAAA AGGATTCTCC TCCACCAGAA 
TGGGATGCTG TGCCAGTTCA CAAGCCCAGC AGCTCTACTG ACAGTCTTTA TAAACTGTCT 
CTGCAAACCC TCAATGCAGA CATTTTCTTA AAACAACGCC AGACCTCGCC AACACCTGCC 
TCTCCTTCTC TGCCCACCGC TCCCTGCCCG TTCACATCTC GTGGCAGCTA CAGCAGCGTT 
GTCAACAGCT CCGGCAGTGA CACAAAAGCC AAGCAGACGA GCAGCAGCAA GAGCAAGCTG 
ACAAAGGCGG CCTCGCTCCC CGGCAAGAAT GGCAACCCTA CCTTCGCCGC CGTCGCTGCA 
GGCTATGACA AGAGCCCGGG TGGGAATGGA TTTGCTAAAA TTTCTTCAAA CAAATCAGAT 
TTTTCCAGCA GCCTTGGCAT TTCACACATT CCTGTTGACA GCGATGGCTC TGACAGCTCG 
GGTTTGTGGA GTCCTGTCAG CAACCCCAAC AGCCCTGACT TCACCCCCCT CAATTCGTTC 
TCAGCCTTTG GAAATTCTTT TAATCTAACA GGTGTTTTCA GCAAACTGTC GCGATCCTGC 
AGTCAGTCGT CCCAGAGAAG TTGGAATGAG TTCAACAGCG GCCCCTCCTA CCTTTGGGAT 
TCTCCAGCCA CAGACCCCAG CCCTTCCTGG CCGGCCAGTT CCAGCTCCCC AACCCACACA 
GCCACTTCCA TCCTTGGCAA CAGCAGCGGC TTGTGGTCCA CCACTCCATT CAGCAGCTCC 
ATCTGGTCCA GCAACATCAA CAGCAACCTT CCTTTCTCCA CTCCAACAAA CGCGCTGTCG 
AGCATCAGCC TCATGGGCAC GGAAAACTCT GCCGCCGCCC ACACCCCTTC CGCCTCTGGC 
CCAGCTGACG ACTTGGGACA GACCTACAAC CCCTGGCGGA TCTGGAGCCC CACAGTCGGA 
AGAAGAAGCT CTGACCCTTG GTCTAATTCG CACTTCCCTC ATGAAAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496213.1
CDS	270..5900
Translation	MGKRAGGAAAAAAAASTSSAAGLEPAAGRGGGPRSAAAGLLGALHLVMTLVVAAARAEKEAFIQSESIIEVLRFDDGGLLQTETTLGLGSYQQKSISLYRGNCRPIRFEPPMLDFHEQPVGMPKMEKVYLHNPSSEETITLVSISATTSHFHASFFQNRKILPGGNTSFDVVFLARVVGNVENTLFINTSNHGVFTYQVFGVGVPNPYRLRPFLGARVPVNSSFSPIINIHNPHSEPLQVVEMYSSGGDLHLELPTGQQGGTRKLWEIPPYETKGVMRASFSSREADNHTAFIRIKTNASDSTEFIILPVEVEVTTAPGIYSSTEMLDFGTLRTQDLPKVLNLHLLNSGTKDVPITSVRPTPQNDAITVHFKPVTLKASESKYTKVASISFDASRAKKPSQFSGKITVKAKEKSYSKLEIPYQAEVLDGYLGFDHAATLFHIQDSPADPVERPIYLTNTFSFAILIHDVLLPEEARIMFQVHNFSQPVLILPNESGYIFTLFFMPSTSSMHIDNNILLVTNASKFHLPVRVYTGFLDYFVLPPKIEERFIDFGVLSATEASSILFAIINSNPIELAIKSWHIIGDGLSIELVATERGNRSTVIASLPELERSSLPDQSPVTLASGHFAVFRVKLTAKKLEGVHDGAIQITTDYEILTIPVKAVIAVGSLTCFPKHMVLPPSFPGKIVHQSLNIMNSFSQKVKIQQIRSLSEDVRFYYKRLRGNREDLEPGKKSKIANIYFDPGLQCGDHCYIGLPFLSKSEPKVQPGVAMQEDLWDADWDAHQSLFKAWMGIKENAGHRLNAMFEVNTDLQKNIVSKVSAELSWPSVLSSPRLLKFPLTNTNCSSEEEISLENPADVPVYVQFIPLALYSNPSVFADKLVSRFNLSKVAKLDLRTLEFQVYRNSAHPLQSPTGFTEGLSRHFILNLILKPGEKKSVKVKFTPLHNRTVSSLIIVRNNLTVMDAVMVQGQGTTENLRVAGKLPGPGSSLRFKITEALLKDCIDRLKLREPNFTLKRTFKVENTGQLEIRVETIEISGYACEGYGFKVVNCQEFALSANASRDIVILFTPDFTASRVIRELKFVTSSGSEFVFVLNASLPYHMLAACAEALPRPNWELALYIIISGVMSALFLLVIGTAYLEAQGIWEPFRRRLSFEASNPPFDVGRPFDLRRIVGISSEGNLNTLGCEHSHGRGFYSNASSRPGTGSHRQCGTSVHPHSSHGSKNSADVDNVRTRNSSSMSSRTSPQAAASQSTSKTSPLVSETAAATQGHTASRKSRGAKQGQHSSQHHSHSHSPLEQHSQPPPPVPQHQEPPPERLSPAPLTHPSHPERASTTRHSSEDSDITSLIEAMDKDFDHHDSSPLDVFTEQPPSPMSKSKGKGKSLQQRKAKPPKKQEEKEKRGKGKPQEDELKDALADDDSSSTTTETSNPDTEPLLREDTEKHKGRPAVPEKQESELSQGKPKSKKLLNAKKEIPTDVKGSSFELPYTPSLENKQRRNLPTKIPLPTTLASGSKSRNPPKTKGTNKLVENRPVALSKFLPSSQELGNTSSSEGEKDSPPPEWDAVPVHKPSSSTDSLYKLSLQTLNADIFLKQRQTSPTPASPSLPTAPCPFTSRGSYSSVVNSSGSDTKAKQTSSSKSKLTKAASLPGKNGNPTFAAVAAGYDKSPGGNGFAKISSNKSDFSSSLGISHIPVDSDGSDSSGLWSPVSNPNSPDFTPLNSFSAFGNSFNLTGVFSKLSRSCSQSSQRSWNEFNSGPSYLWDSPATDPSPSWPASSSSPTHTATSILGNSSGLWSTTPFSSSIWSSNINSNLPFSTPTNALSSISLMGTENSAAAHTPSASGPADDLGQTYNPWRIWSPTVGRRSSDPWSNSHFPHEN

Target ORF information:

RefSeq Version	XM_006496150.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane protein 131 (Tmem131), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496150.3

ORF Insert Sequence:

ATGGGGAAGC GGGCGGGAGG AGCAGCCGCC GCCGCCGCCG CCGCCTCCAC GTCCTCCGCG 
GCCGGGCTGG AGCCCGCGGC TGGCCGAGGC GGGGGTCCTC GGAGCGCAGC CGCGGGCCTC 
CTGGGAGCGC TGCACCTGGT GATGACCCTC GTGGTGGCCG CGGCGCGGGC CGAGAAGGAA 
GCATTCATTC AGTCGGAGAG CATAATAGAA GTCCTGCGCT TTGACGACGG AGGGCTACTA 
CAGACTGAGA CAACACTTGG CCTCGGTTCA TATCAACAGA AAAGCATTTC TCTGTACCGG 
GGTAATTGCA GGCCCATACG ATTCGAACCG CCCATGCTGG ATTTCCATGA GCAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTCTACTTA CATAACCCTA GTTCTGAAGA AACGATTACT 
TTAGTATCAA TATCTGCTAC CACATCACAT TTCCATGCAT CGTTTTTTCA AAATAGGAAA 
ATTCTTCCAG GAGGAAACAC ATCATTTGAT GTAGTTTTTC TTGCAAGAGT AGTAGGAAAT 
GTAGAAAATA CTTTATTTAT TAACACATCT AACCATGGGG TGTTCACTTA CCAGGTATTT 
GGTGTTGGGG TTCCAAATCC GTATCGATTA AGGCCATTTC TTGGGGCCAG AGTCCCCGTA 
AACAGCAGCT TTTCACCCAT AATAAACATA CACAATCCTC ACAGTGAGCC TTTACAGGTC 
GTAGAGATGT ACTCGAGTGG AGGTGACCTT CACCTAGAGC TCCCAACAGG TCAGCAAGGA 
GGCACCAGGA AGCTGTGGGA AATCCCTCCT TATGAAACCA AGGGAGTGAT GAGAGCCAGC 
TTTTCCTCCA GAGAAGCAGA CAATCACACA GCCTTCATCC GGATAAAGAC GAATGCATCA 
GACAGCACCG AGTTCATCAT TCTCCCGGTT GAGGTTGAAG TTACAACAGC TCCTGGAATC 
TATTCCTCAA CTGAAATGTT AGATTTTGGA ACACTTAGAA CACAAGATCT ACCAAAGGTT 
TTAAATCTTC ATTTACTAAA TTCAGGAACA AAGGATGTAC CAATAACAAG TGTTCGACCA 
ACACCACAAA ATGACGCTAT CACGGTACAT TTTAAACCAG TTACGTTAAA AGCTTCTGAA 
AGTAAATATA CCAAGGTTGC CAGCATTAGT TTTGATGCTT CGAGAGCAAA AAAGCCATCT 
CAATTTTCTG GGAAAATAAC TGTTAAAGCA AAGGAAAAGA GTTATTCTAA ACTCGAAATC 
CCCTACCAAG CAGAAGTTCT AGATGGTTAT TTGGGGTTTG ATCACGCCGC CACGCTGTTT 
CACATCCAGG ACAGTCCTGC TGACCCAGTG GAGAGGCCGA TCTACCTGAC GAACACTTTC 
AGTTTTGCGA TCCTCATTCA TGATGTGCTG TTACCAGAAG AAGCCAGAAT AATGTTTCAG 
GTGCACAACT TCAGCCAGCC GGTTCTCATT CTTCCTAATG AGTCGGGATA CATTTTTACC 
CTGTTTTTTA TGCCATCCAC CTCATCCATG CACATAGACA ACAACATTTT ACTTGTTACC 
AATGCTTCTA AGTTTCATTT ACCTGTGCGG GTATACACAG GCTTTTTAGA TTACTTTGTA 
TTGCCCCCCA AAATAGAAGA ACGCTTCATA GATTTTGGAG TACTGAGTGC TACAGAAGCA 
AGTAGTATTT TATTTGCAAT TATAAACAGC AATCCAATTG AGCTGGCAAT AAAAAGTTGG 
CATATCATAG GAGATGGCTT ATCAATAGAA CTTGTAGCTA CAGAGAGAGG CAACAGAAGC 
ACGGTGATTG CAAGCCTTCC AGAACTGGAG CGGTCCTCTT TGCCCGACCA GTCCCCGGTA 
ACATTAGCTT CAGGACACTT TGCAGTGTTC AGAGTCAAAC TTACTGCCAA GAAGCTAGAA 
GGGGTCCATG ATGGGGCCAT ACAGATCACA ACCGACTACG AGATCCTGAC CATCCCTGTG 
AAAGCTGTGA TCGCCGTGGG CTCGCTGACC TGCTTCCCCA AGCACATGGT TCTTCCCCCT 
TCCTTCCCAG GGAAAATAGT TCATCAGAGC TTAAATATTA TGAATTCCTT CTCACAGAAG 
GTGAAGATAC AGCAAATAAG GTCTTTGTCA GAAGACGTAC GATTCTACTA CAAACGATTA 
CGGGGTAATA GGGAAGACTT GGAGCCAGGG AAGAAGTCTA AGATTGCAAA CATTTATTTT 
GACCCTGGGC TGCAGTGTGG GGATCACTGC TATATCGGCT TGCCTTTCCT ATCCAAATCT 
GAACCCAAAG TACAGCCTGG GGTGGCCATG CAGGAGGACT TGTGGGATGC CGACTGGGAC 
GCACATCAGA GCTTGTTCAA GGCTTGGATG GGGATAAAGG AAAACGCAGG ACATAGGTTG 
AATGCTATGT TTGAAGTAAA TACAGATCTT CAGAAAAATA TCGTATCAAA AGTCAGTGCC 
GAGCTCTCCT GGCCGTCTGT CCTTAGCTCG CCCCGGCTCC TGAAGTTCCC ACTCACTAAC 
ACAAACTGTT CTTCGGAAGA AGAGATTAGC TTAGAAAACC CTGCAGACGT CCCTGTCTAC 
GTCCAGTTCA TCCCACTGGC TTTGTATTCC AACCCCTCCG TCTTTGCAGA TAAGTTAGTC 
TCAAGGTTTA ACTTGAGTAA GGTGGCCAAG CTAGATTTGA GAACACTTGA ATTCCAAGTC 
TACAGGAACA GCGCTCATCC ACTGCAAAGT CCAACAGGAT TTACAGAGGG TCTCTCTCGG 
CATTTCATTT TAAACCTCAT TTTAAAACCT GGAGAAAAGA AATCTGTCAA AGTAAAGTTC 
ACTCCACTCC ACAACAGAAC TGTGTCTTCC CTGATCATAG TCAGAAACAA CCTGACTGTG 
ATGGACGCTG TGATGGTTCA AGGTCAAGGG ACAACGGAGA ACTTGAGGGT GGCAGGCAAG 
CTTCCAGGTC CAGGAAGCTC CTTACGCTTC AAAATCACAG AAGCATTGTT AAAGGATTGC 
ATAGACCGTT TGAAACTAAG AGAACCAAAT TTCACATTGA AAAGAACATT CAAAGTAGAG 
AATACGGGAC AACTTGAAAT CCGTGTAGAA ACCATTGAGA TCAGTGGATA TGCGTGTGAG 
GGATATGGTT TTAAAGTGGT TAACTGTCAA GAGTTTGCCC TGAGTGCCAA CGCCTCCAGA 
GACATAGTCA TATTGTTTAC TCCAGATTTC ACAGCCTCCA GAGTCATTCG GGAGCTGAAG 
TTTGTGACAA GCAGTGGCTC CGAGTTTGTG TTTGTGTTGA ATGCCTCTCT TCCGTACCAC 
ATGCTAGCCG CCTGTGCAGA AGCCCTCCCT AGACCCAACT GGGAGCTCGC GCTCTACATC 
ATCATCTCCG GGGTCATGAG TGCACTCTTT CTCCTGGTCA TTGGAACAGC CTACTTGGAA 
GCTCAAGGGA TTTGGGAGCC CTTCCGAAGG CGACTCTCCT TTGAAGCCTC AAACCCGCCC 
TTTGATGTAG GAAGGCCATT TGATCTCAGG AGAATCGTTG GTATTTCATC TGAAGGAAAC 
TTGAACACAC TTGGCTGCGA ACACAGTCAT GGTAGGGGTT TCTATAGTAA CGCATCATCC 
CGGCCTGGTA CAGGCAGTCA CAGGCAGTGT GGCACATCAG TCCACCCACA CAGCAGCCAT 
GGCAGCAAAA ACTCAGCTGA TGTGGACAAC GTCAGAACCA GAAACAGTTC AAGTATGTCA 
AGCAGGACTT CTCCACAAGC AGCTGCCTCA CAGTCTACAA GCAAAACCAG CCCCCTAGTC 
TCAGAGACAG CTGCAGCAAC CCAGGGTCAC ACAGCAAGCA GGAAGTCCAG AGGGGCAAAG 
CAGGGCCAGC ACAGCAGCCA GCACCACAGC CACAGCCACA GCCCACTGGA GCAGCACTCA 
CAGCCTCCAC CACCAGTGCC TCAGCACCAG GAGCCACCAC CCGAAAGGCT GTCTCCTGCC 
CCCCTCACAC ACCCTTCCCA CCCCGAGCGA GCCAGCACCA CACGGCACAG CTCAGAGGAC 
TCCGACATCA CCAGTCTCAT AGAAGCCATG GACAAAGACT TTGACCACCA CGACTCCTCC 
CCCCTAGACG TGTTTACAGA GCAGCCTCCG TCACCGATGT CAAAAAGCAA AGGGAAAGGA 
AAGTCTCTCC AACAACGTAA GGCCAAGCCT CCGAAGAAGC AGGAGGAGAA GGAGAAGAGG 
GGGAAGGGGA AGCCGCAGGA GGATGAGCTG AAGGACGCTC TGGCCGACGA CGACAGCTCC 
TCCACCACCA CAGAGACCTC CAACCCCGAC ACAGAGCCAC TCCTCAGGGA GGATACAGAG 
AAGCACAAGG GGAGACCAGC CGTGCCTGAA AAACAGGAAA GTGAACTGTC TCAAGGGAAG 
CCCAAGAGCA AAAAGCTCTT AAACGCTAAG AAAGAAATCC CAACAGATGT GAAAGGCAGT 
TCCTTTGAAC TACCGTACAC TCCCTCACTG GAAAATAAAC AGCGTAGAAA TCTCCCAACC 
AAGATTCCTC TTCCAACTAC ACTAGCAAGT GGATCCAAAT CACGAAATCC CCCGAAAACA 
AAAGGTACAA ATAAGTTAGT GGAAAACAGG CCAGTTGCCC TCTCAAAGTT CCTCCCCAGC 
AGTCAAGAAC TAGGTAATAC CAGCAGCTCA GAGGGTGAAA AGGATTCTCC TCCACCAGAA 
TGGGATGCTG TGCCAGTTCA CAAGCCCAGC AGCTCTACTG ACAGTCTTTA TAAACTGTCT 
CTGCAAACCC TCAATGCAGA CATTTTCTTA AAACAACGCC AGACCTCGCC AACACCTGCC 
TCTCCTTCTC TGCCCACCGC TCCCTGCCCG TTCACATCTC GTGGCAGCTA CAGCAGCGTT 
GTCAACAGCT CCGGCAGTGA CACAAAAGCC AAGCAGACGA GCAGCAGCAA GAGCAAGCTG 
ACAAAGGCGG CCTCGCTCCC CGGCAAGAAT GGCAACCCTA CCTTCGCCGC CGTCGCTGCA 
GGCTATGACA AGAGCCCGGG TGGGAATGGA TTTGCTAAAA TTTCTTCAAA CAAATCAGAT 
TTTTCCAGCA GCCTTGGCAT TTCACACATT CCTGTTGACA GCGATGGCTC TGACAGCTCG 
GGTTTGTGGA GTCCTGTCAG CAACCCCAAC AGCCCTGACT TCACCCCCCT CAATTCGTTC 
TCAGCCTTTG GAAATTCTTT TAATCTAACA GGTGTTTTCA GCAAACTGTC GCGATCCTGC 
AGTCAGTCGT CCCAGAGAAG TTGGAATGAG TTCAACAGCG GCCCCTCCTA CCTTTGGGAT 
TCTCCAGCCA CAGACCCCAG CCCTTCCTGG CCGGCCAGTT CCAGCTCCCC AACCCACACA 
GCCACTTCCA TCCTTGGCAA CAGCAGCGGC TTGTGGTCCA CCACTCCATT CAGCAGCTCC 
ATCTGGTCCA GCAACATCAA CAGCAACCTT CCTTTCTCCA CTCCAACAAA CGCGCTGTCG 
AGCATCAGCC TCATGGGCAC GGAAAACTCT GCCGCCGCCC ACACCCCTTC CGCCTCTGGC 
CCAGCTGACG ACTTGGGACA GACCTACAAC CCCTGGCGGA TCTGGAGCCC CACAGTCGGA 
AGAAGAAGCT CTGACCCTTG GTCTAATTCG CACTTCCCTC ATGAAAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmem131 ( XM_006496152.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496215.1 Mus musculus transmembrane protein 131 (Tmem131), transcript variant X3, mRNA.

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CloneID	OMu27536
Clone ID Related Accession (Same CDS sequence)	XM_006496152.2 , XM_006496152.3
Accession Version	XM_006496152.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	transmembrane protein 131 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496152.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGC GGGCGGGAGG AGCAGCCGCC GCCGCCGCCG CCGCCTCCAC GTCCTCCGCG 
GCCGGGCTGG AGCCCGCGGC TGGCCGAGGC GGGGGTCCTC GGAGCGCAGC CGCGGGCCTC 
CTGGGAGCGC TGCACCTGGT GATGACCCTC GTGGTGGCCG CGGCGCGGGC CGAGAAGGAA 
GCATTCATTC AGTCGGAGAG CATAATAGAA GTCCTGCGCT TTGACGACGG AGGGCTACTA 
CAGACTGAGA CAACACTTGG CCTCGGTTCA TATCAACAGA AAAGCATTTC TCTGTACCGG 
GGTAATTGCA GGCCCATACG ATTCGAACCG CCCATGCTGG ATTTCCATGA GCAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTCTACTTA CATAACCCTA GTTCTGAAGA AACGATTACT 
TTAGTATCAA TATCTGCTAC CACATCACAT TTCCATGCAT CGTTTTTTCA AAATAGGAAA 
ATTCTTCCAG GAGGAAACAC ATCATTTGAT GTAGTTTTTC TTGCAAGAGT AGTAGGAAAT 
GTAGAAAATA CTTTATTTAT TAACACATCT AACCATGGGG TGTTCACTTA CCAGGTATTT 
GGTGTTGGGG TTCCAAATCC GTATCGATTA AGGCCATTTC TTGGGGCCAG AGTCCCCGTA 
AACAGCAGCT TTTCACCCAT AATAAACATA CACAATCCTC ACAGTGAGCC TTTACAGGTC 
GTAGAGATGT ACTCGAGTGG AGGTGACCTT CACCTAGAGC TCCCAACAGG TCAGCAAGGA 
GGCACCAGGA AGCTGTGGGA AATCCCTCCT TATGAAACCA AGGGAGTGAT GAGAGCCAGC 
TTTTCCTCCA GAGAAGCAGA CAATCACACA GCCTTCATCC GGATAAAGAC GAATGCATCA 
GACAGCACCG AGTTCATCAT TCTCCCGGTT GAGGTTGAAG TTACAACAGC TCCTGGAATC 
TATTCCTCAA CTGAAATGTT AGATTTTGGA ACACTTAGAA CACAAGATCT ACCAAAGGTT 
TTAAATCTTC ATTTACTAAA TTCAGGAACA AAGGATGTAC CAATAACAAG TGTTCGACCA 
ACACCACAAA ATGACGCTAT CACGGTACAT TTTAAACCAG TTACGTTAAA AGCTTCTGAA 
AGTAAATATA CCAAGGTTGC CAGCATTAGT TTTGATGCTT CGAGAGCAAA AAAGCCATCT 
CAATTTTCTG GGAAAATAAC TGTTAAAGCA AAGGAAAAGA GTTATTCTAA ACTCGAAATC 
CCCTACCAAG CAGAAGTTCT AGATGGTTAT TTGGGGTTTG ATCACGCCGC CACGCTGTTT 
CACATCCAGG ACAGTCCTGC TGACCCAGTG GAGAGGCCGA TCTACCTGAC GAACACTTTC 
AGTTTTGCGA TCCTCATTCA TGATGTGCTG TTACCAGAAG AAGCCAGAAT AATGTTTCAG 
GTGCACAACT TCAGCCAGCC GGTTCTCATT CTTCCTAATG AGTCGGGATA CATTTTTACC 
CTGTTTTTTA TGCCATCCAC CTCATCCATG CACATAGACA ACAACATTTT ACTTGTTACC 
AATGCTTCTA AGTTTCATTT ACCTGTGCGG GTATACACAG GCTTTTTAGA TTACTTTGTA 
TTGCCCCCCA AAATAGAAGA ACGCTTCATA GATTTTGGAG TACTGAGTGC TACAGAAGCA 
AGTAGTATTT TATTTGCAAT TATAAACAGC AATCCAATTG AGCTGGCAAT AAAAAGTTGG 
CATATCATAG GAGATGGCTT ATCAATAGAA CTTGTAGCTA CAGAGAGAGG CAACAGAAGC 
ACGGTGATTG CAAGCCTTCC AGAACTGGAG CGGTCCTCTT TGCCCGACCA GTCCCCGGTA 
ACATTAGCTT CAGGACACTT TGCAGTGTTC AGAGTCAAAC TTACTGCCAA GAAGCTAGAA 
GGGGTCCATG ATGGGGCCAT ACAGATCACA ACCGACTACG AGATCCTGAC CATCCCTGTG 
AAAGCTGTGA TCGCCGTGGG CTCGCTGACC TGCTTCCCCA AGCACATGGT TCTTCCCCCT 
TCCTTCCCAG GGAAAATAGT TCATCAGAGC TTAAATATTA TGAATTCCTT CTCACAGAAG 
GTGAAGATAC AGCAAATAAG GTCTTTGTCA GAAGACGTAC GATTCTACTA CAAACGATTA 
CGGGGTAATA GGGAAGACTT GGAGCCAGGG AAGAAGTCTA AGATTGCAAA CATTTATTTT 
GACCCTGGGC TGCAGTGTGG GGATCACTGC TATATCGGCT TGCCTTTCCT ATCCAAATCT 
GAACCCAAAG TACAGCCTGG GGTGGCCATG CAGGAGGACT TGTGGGATGC CGACTGGGAC 
GCACATCAGA GCTTGTTCAA GGCTTGGATG GGGATAAAGG AAAACGCAGG ACATAGGTTG 
AATGCTATGT TTGAAGTAAA TACAGATCTT CAGAAAAATA TCGTATCAAA AGTCAGTGCC 
GAGCTCTCCT GGCCGTCTGT CCTTAGCTCG CCCCGGCTCC TGAAGTTCCC ACTCACTAAC 
ACAAACTGTT CTTCGGAAGA AGAGATTAGC TTAGAAAACC CTGCAGACGT CCCTGTCTAC 
GTCCAGTTCA TCCCACTGGC TTTGTATTCC AACCCCTCCG TCTTTGCAGA TAAGTTAGTC 
TCAAGGTTTA ACTTGAGTAA GGTGGCCAAG CTAGATTTGA GAACACTTGA ATTCCAAGTC 
TACAGGAACA GCGCTCATCC ACTGCAAAGT CCAACAGGAT TTACAGAGGG TCTCTCTCGG 
CATTTCATTT TAAACCTCAT TTTAAAACCT GGAGAAAAGA AATCTGTCAA AGTAAAGTTC 
ACTCCACTCC ACAACAGAAC TGTGTCTTCC CTGATCATAG TCAGAAACAA CCTGACTGTG 
ATGGACGCTG TGATGGTTCA AGGTCAAGGG ACAACGGAGA ACTTGAGGGT GGCAGGCAAG 
CTTCCAGGTC CAGGAAGCTC CTTACGCTTC AAAATCACAG AAGCATTGTT AAAGGATTGC 
ATAGACCGTT TGAAACTAAG AGAACCAAAT TTCACATTGA AAAGAACATT CAAAGTAGAG 
AATACGGGAC AACTTGAAAT CCGTGTAGAA ACCATTGAGA TCAGTGGATA TGCGTGTGAG 
GGATATGGTT TTAAAGTGGT TAACTGTCAA GAGTTTGCCC TGAGTGCCAA CGCCTCCAGA 
GACATAGTCA TATTGTTTAC TCCAGATTTC ACAGCCTCCA GAGTCATTCG GGAGCTGAAG 
TTTGTGACAA GCAGTGGCTC CGAGTTTGTG TTTGTGTTGA ATGCCTCTCT TCCGTACCAC 
ATGCTAGCCG CCTGTGCAGA AGCCCTCCCT AGACCCAACT GGGAGCTCGC GCTCTACATC 
ATCATCTCCG GGGTCATGAG TGCACTCTTT CTCCTGGTCA TTGGAACAGC CTACTTGGAA 
GCTCAAGGGA TTTGGGAGCC CTTCCGAAGG CGACTCTCCT TTGAAGCCTC AAACCCGCCC 
TTTGATGTAG GAAGGCCATT TGATCTCAGG AGAATCGTTG GTATTTCATC TGAAGGAAAC 
TTGAACACAC TTGGCTGCGA ACACAGTCAT GGTAGGGGTT TCTATAGTAA CGCATCATCC 
CGGCCTGGTA CAGGCAGTCA CAGGCAGTGT GGCACATCAG TCCACCCACA CAGCAGCCAT 
GGCAGCAAAA ACTCAGCTGA TGTGGACAAC GTCAGAACCA GAAACAGTTC AAGTATGTCA 
AGCAGGACTT CTCCACAAGC AGCTGCCTCA CAGTCTACAA GCAAAACCAG CCCCCTAGTC 
TCAGAGACAG CTGCAGCAAC CCAGGGTCAC ACAGCAAGCA GGAAGTCCAG AGGGGCAAAG 
CAGGGCCAGC ACAGCAGCCA GCACCACAGC CACAGCCACA GCCCACTGGA GCAGCACTCA 
CAGCCTCCAC CACCAGTGCC TCAGCACCAG GAGCCACCAC CCGAAAGGCT GTCTCCTGCC 
CCCCTCACAC ACCCTTCCCA CCCCGAGCGA GCCAGCACCA CACGGCACAG CTCAGAGGAC 
TCCGACATCA CCAGTCTCAT AGAAGCCATG GACAAAGACT TTGACCACCA CGACTCCTCC 
CCCCTAGACG TGTTTACAGA GCAGCCTCCG TCACCGATGT CAAAAAGCAA AGGGAAAGGA 
AAGTCTCTCC AACAACGTAA GGCCAAGCCT CCGAAGAAGC AGGAGGAGAA GGAGAAGAGG 
GGGAAGGGGA AGCCGCAGGA GGATGAGCTG AAGGACGCTC TGGCCGACGA CGACAGCTCC 
TCCACCACCA CAGAGACCTC CAACCCCGAC ACAGAGCCAC TCCTCAGGGA GGATACAGAG 
AAGCACAAGG GGAGACCAGC CGTGCCTGAA AAACAGGAAA GTGAACTGTC TCAAGGGAAG 
CCCAAGAGCA AAAAGCTCTT AAACGCTAAG AAAGAAATCC CAACAGATGT GAAAGGCAGT 
TCCTTTGAAC TACCGTACAC TCCCTCACTG GAAAATAAAC AGCGTAGAAA TCTCCCAACC 
AAGATTCCTC TTCCAACTAC ACTAGCAAGT GGATCCAAAT CACGAAATCC CCCGAAAACA 
AAAGGTACAA ATAAGTTAGT GGAAAACAGG CCAGTTGCCC TCTCAAAGTT CCTCCCCAGC 
AGTCAAGAAC TAGGTAATAC CAGCAGCTCA GAGGGTGAAA AGGATTCTCC TCCACCAGAA 
TGGGATGCTG TGCCAGTTCA CAAGCCCAGC AGCTCTACTG ACAGTCTTTA TAAACTGTCT 
CTGCAAACCC TCAATGCAGA CATTTTCTTA AAACAACGCC AGACCTCGCC AACACCTGCC 
TCTCCTTCTC TGCCCACCGC TCCCTGCCCG TTCACATCTC GTGGCAGCTA CAGCAGCGTT 
GTCAACAGCT CCGGCAGGTA CCTGACCCCA GCACTGACCT TATCCTGTCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496215.1
CDS	270..5183
Translation	MGKRAGGAAAAAAAASTSSAAGLEPAAGRGGGPRSAAAGLLGALHLVMTLVVAAARAEKEAFIQSESIIEVLRFDDGGLLQTETTLGLGSYQQKSISLYRGNCRPIRFEPPMLDFHEQPVGMPKMEKVYLHNPSSEETITLVSISATTSHFHASFFQNRKILPGGNTSFDVVFLARVVGNVENTLFINTSNHGVFTYQVFGVGVPNPYRLRPFLGARVPVNSSFSPIINIHNPHSEPLQVVEMYSSGGDLHLELPTGQQGGTRKLWEIPPYETKGVMRASFSSREADNHTAFIRIKTNASDSTEFIILPVEVEVTTAPGIYSSTEMLDFGTLRTQDLPKVLNLHLLNSGTKDVPITSVRPTPQNDAITVHFKPVTLKASESKYTKVASISFDASRAKKPSQFSGKITVKAKEKSYSKLEIPYQAEVLDGYLGFDHAATLFHIQDSPADPVERPIYLTNTFSFAILIHDVLLPEEARIMFQVHNFSQPVLILPNESGYIFTLFFMPSTSSMHIDNNILLVTNASKFHLPVRVYTGFLDYFVLPPKIEERFIDFGVLSATEASSILFAIINSNPIELAIKSWHIIGDGLSIELVATERGNRSTVIASLPELERSSLPDQSPVTLASGHFAVFRVKLTAKKLEGVHDGAIQITTDYEILTIPVKAVIAVGSLTCFPKHMVLPPSFPGKIVHQSLNIMNSFSQKVKIQQIRSLSEDVRFYYKRLRGNREDLEPGKKSKIANIYFDPGLQCGDHCYIGLPFLSKSEPKVQPGVAMQEDLWDADWDAHQSLFKAWMGIKENAGHRLNAMFEVNTDLQKNIVSKVSAELSWPSVLSSPRLLKFPLTNTNCSSEEEISLENPADVPVYVQFIPLALYSNPSVFADKLVSRFNLSKVAKLDLRTLEFQVYRNSAHPLQSPTGFTEGLSRHFILNLILKPGEKKSVKVKFTPLHNRTVSSLIIVRNNLTVMDAVMVQGQGTTENLRVAGKLPGPGSSLRFKITEALLKDCIDRLKLREPNFTLKRTFKVENTGQLEIRVETIEISGYACEGYGFKVVNCQEFALSANASRDIVILFTPDFTASRVIRELKFVTSSGSEFVFVLNASLPYHMLAACAEALPRPNWELALYIIISGVMSALFLLVIGTAYLEAQGIWEPFRRRLSFEASNPPFDVGRPFDLRRIVGISSEGNLNTLGCEHSHGRGFYSNASSRPGTGSHRQCGTSVHPHSSHGSKNSADVDNVRTRNSSSMSSRTSPQAAASQSTSKTSPLVSETAAATQGHTASRKSRGAKQGQHSSQHHSHSHSPLEQHSQPPPPVPQHQEPPPERLSPAPLTHPSHPERASTTRHSSEDSDITSLIEAMDKDFDHHDSSPLDVFTEQPPSPMSKSKGKGKSLQQRKAKPPKKQEEKEKRGKGKPQEDELKDALADDDSSSTTTETSNPDTEPLLREDTEKHKGRPAVPEKQESELSQGKPKSKKLLNAKKEIPTDVKGSSFELPYTPSLENKQRRNLPTKIPLPTTLASGSKSRNPPKTKGTNKLVENRPVALSKFLPSSQELGNTSSSEGEKDSPPPEWDAVPVHKPSSSTDSLYKLSLQTLNADIFLKQRQTSPTPASPSLPTAPCPFTSRGSYSSVVNSSGRYLTPALTLSCL

Target ORF information:

RefSeq Version	XM_006496152.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane protein 131 (Tmem131), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496152.3

ORF Insert Sequence:

ATGGGGAAGC GGGCGGGAGG AGCAGCCGCC GCCGCCGCCG CCGCCTCCAC GTCCTCCGCG 
GCCGGGCTGG AGCCCGCGGC TGGCCGAGGC GGGGGTCCTC GGAGCGCAGC CGCGGGCCTC 
CTGGGAGCGC TGCACCTGGT GATGACCCTC GTGGTGGCCG CGGCGCGGGC CGAGAAGGAA 
GCATTCATTC AGTCGGAGAG CATAATAGAA GTCCTGCGCT TTGACGACGG AGGGCTACTA 
CAGACTGAGA CAACACTTGG CCTCGGTTCA TATCAACAGA AAAGCATTTC TCTGTACCGG 
GGTAATTGCA GGCCCATACG ATTCGAACCG CCCATGCTGG ATTTCCATGA GCAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTCTACTTA CATAACCCTA GTTCTGAAGA AACGATTACT 
TTAGTATCAA TATCTGCTAC CACATCACAT TTCCATGCAT CGTTTTTTCA AAATAGGAAA 
ATTCTTCCAG GAGGAAACAC ATCATTTGAT GTAGTTTTTC TTGCAAGAGT AGTAGGAAAT 
GTAGAAAATA CTTTATTTAT TAACACATCT AACCATGGGG TGTTCACTTA CCAGGTATTT 
GGTGTTGGGG TTCCAAATCC GTATCGATTA AGGCCATTTC TTGGGGCCAG AGTCCCCGTA 
AACAGCAGCT TTTCACCCAT AATAAACATA CACAATCCTC ACAGTGAGCC TTTACAGGTC 
GTAGAGATGT ACTCGAGTGG AGGTGACCTT CACCTAGAGC TCCCAACAGG TCAGCAAGGA 
GGCACCAGGA AGCTGTGGGA AATCCCTCCT TATGAAACCA AGGGAGTGAT GAGAGCCAGC 
TTTTCCTCCA GAGAAGCAGA CAATCACACA GCCTTCATCC GGATAAAGAC GAATGCATCA 
GACAGCACCG AGTTCATCAT TCTCCCGGTT GAGGTTGAAG TTACAACAGC TCCTGGAATC 
TATTCCTCAA CTGAAATGTT AGATTTTGGA ACACTTAGAA CACAAGATCT ACCAAAGGTT 
TTAAATCTTC ATTTACTAAA TTCAGGAACA AAGGATGTAC CAATAACAAG TGTTCGACCA 
ACACCACAAA ATGACGCTAT CACGGTACAT TTTAAACCAG TTACGTTAAA AGCTTCTGAA 
AGTAAATATA CCAAGGTTGC CAGCATTAGT TTTGATGCTT CGAGAGCAAA AAAGCCATCT 
CAATTTTCTG GGAAAATAAC TGTTAAAGCA AAGGAAAAGA GTTATTCTAA ACTCGAAATC 
CCCTACCAAG CAGAAGTTCT AGATGGTTAT TTGGGGTTTG ATCACGCCGC CACGCTGTTT 
CACATCCAGG ACAGTCCTGC TGACCCAGTG GAGAGGCCGA TCTACCTGAC GAACACTTTC 
AGTTTTGCGA TCCTCATTCA TGATGTGCTG TTACCAGAAG AAGCCAGAAT AATGTTTCAG 
GTGCACAACT TCAGCCAGCC GGTTCTCATT CTTCCTAATG AGTCGGGATA CATTTTTACC 
CTGTTTTTTA TGCCATCCAC CTCATCCATG CACATAGACA ACAACATTTT ACTTGTTACC 
AATGCTTCTA AGTTTCATTT ACCTGTGCGG GTATACACAG GCTTTTTAGA TTACTTTGTA 
TTGCCCCCCA AAATAGAAGA ACGCTTCATA GATTTTGGAG TACTGAGTGC TACAGAAGCA 
AGTAGTATTT TATTTGCAAT TATAAACAGC AATCCAATTG AGCTGGCAAT AAAAAGTTGG 
CATATCATAG GAGATGGCTT ATCAATAGAA CTTGTAGCTA CAGAGAGAGG CAACAGAAGC 
ACGGTGATTG CAAGCCTTCC AGAACTGGAG CGGTCCTCTT TGCCCGACCA GTCCCCGGTA 
ACATTAGCTT CAGGACACTT TGCAGTGTTC AGAGTCAAAC TTACTGCCAA GAAGCTAGAA 
GGGGTCCATG ATGGGGCCAT ACAGATCACA ACCGACTACG AGATCCTGAC CATCCCTGTG 
AAAGCTGTGA TCGCCGTGGG CTCGCTGACC TGCTTCCCCA AGCACATGGT TCTTCCCCCT 
TCCTTCCCAG GGAAAATAGT TCATCAGAGC TTAAATATTA TGAATTCCTT CTCACAGAAG 
GTGAAGATAC AGCAAATAAG GTCTTTGTCA GAAGACGTAC GATTCTACTA CAAACGATTA 
CGGGGTAATA GGGAAGACTT GGAGCCAGGG AAGAAGTCTA AGATTGCAAA CATTTATTTT 
GACCCTGGGC TGCAGTGTGG GGATCACTGC TATATCGGCT TGCCTTTCCT ATCCAAATCT 
GAACCCAAAG TACAGCCTGG GGTGGCCATG CAGGAGGACT TGTGGGATGC CGACTGGGAC 
GCACATCAGA GCTTGTTCAA GGCTTGGATG GGGATAAAGG AAAACGCAGG ACATAGGTTG 
AATGCTATGT TTGAAGTAAA TACAGATCTT CAGAAAAATA TCGTATCAAA AGTCAGTGCC 
GAGCTCTCCT GGCCGTCTGT CCTTAGCTCG CCCCGGCTCC TGAAGTTCCC ACTCACTAAC 
ACAAACTGTT CTTCGGAAGA AGAGATTAGC TTAGAAAACC CTGCAGACGT CCCTGTCTAC 
GTCCAGTTCA TCCCACTGGC TTTGTATTCC AACCCCTCCG TCTTTGCAGA TAAGTTAGTC 
TCAAGGTTTA ACTTGAGTAA GGTGGCCAAG CTAGATTTGA GAACACTTGA ATTCCAAGTC 
TACAGGAACA GCGCTCATCC ACTGCAAAGT CCAACAGGAT TTACAGAGGG TCTCTCTCGG 
CATTTCATTT TAAACCTCAT TTTAAAACCT GGAGAAAAGA AATCTGTCAA AGTAAAGTTC 
ACTCCACTCC ACAACAGAAC TGTGTCTTCC CTGATCATAG TCAGAAACAA CCTGACTGTG 
ATGGACGCTG TGATGGTTCA AGGTCAAGGG ACAACGGAGA ACTTGAGGGT GGCAGGCAAG 
CTTCCAGGTC CAGGAAGCTC CTTACGCTTC AAAATCACAG AAGCATTGTT AAAGGATTGC 
ATAGACCGTT TGAAACTAAG AGAACCAAAT TTCACATTGA AAAGAACATT CAAAGTAGAG 
AATACGGGAC AACTTGAAAT CCGTGTAGAA ACCATTGAGA TCAGTGGATA TGCGTGTGAG 
GGATATGGTT TTAAAGTGGT TAACTGTCAA GAGTTTGCCC TGAGTGCCAA CGCCTCCAGA 
GACATAGTCA TATTGTTTAC TCCAGATTTC ACAGCCTCCA GAGTCATTCG GGAGCTGAAG 
TTTGTGACAA GCAGTGGCTC CGAGTTTGTG TTTGTGTTGA ATGCCTCTCT TCCGTACCAC 
ATGCTAGCCG CCTGTGCAGA AGCCCTCCCT AGACCCAACT GGGAGCTCGC GCTCTACATC 
ATCATCTCCG GGGTCATGAG TGCACTCTTT CTCCTGGTCA TTGGAACAGC CTACTTGGAA 
GCTCAAGGGA TTTGGGAGCC CTTCCGAAGG CGACTCTCCT TTGAAGCCTC AAACCCGCCC 
TTTGATGTAG GAAGGCCATT TGATCTCAGG AGAATCGTTG GTATTTCATC TGAAGGAAAC 
TTGAACACAC TTGGCTGCGA ACACAGTCAT GGTAGGGGTT TCTATAGTAA CGCATCATCC 
CGGCCTGGTA CAGGCAGTCA CAGGCAGTGT GGCACATCAG TCCACCCACA CAGCAGCCAT 
GGCAGCAAAA ACTCAGCTGA TGTGGACAAC GTCAGAACCA GAAACAGTTC AAGTATGTCA 
AGCAGGACTT CTCCACAAGC AGCTGCCTCA CAGTCTACAA GCAAAACCAG CCCCCTAGTC 
TCAGAGACAG CTGCAGCAAC CCAGGGTCAC ACAGCAAGCA GGAAGTCCAG AGGGGCAAAG 
CAGGGCCAGC ACAGCAGCCA GCACCACAGC CACAGCCACA GCCCACTGGA GCAGCACTCA 
CAGCCTCCAC CACCAGTGCC TCAGCACCAG GAGCCACCAC CCGAAAGGCT GTCTCCTGCC 
CCCCTCACAC ACCCTTCCCA CCCCGAGCGA GCCAGCACCA CACGGCACAG CTCAGAGGAC 
TCCGACATCA CCAGTCTCAT AGAAGCCATG GACAAAGACT TTGACCACCA CGACTCCTCC 
CCCCTAGACG TGTTTACAGA GCAGCCTCCG TCACCGATGT CAAAAAGCAA AGGGAAAGGA 
AAGTCTCTCC AACAACGTAA GGCCAAGCCT CCGAAGAAGC AGGAGGAGAA GGAGAAGAGG 
GGGAAGGGGA AGCCGCAGGA GGATGAGCTG AAGGACGCTC TGGCCGACGA CGACAGCTCC 
TCCACCACCA CAGAGACCTC CAACCCCGAC ACAGAGCCAC TCCTCAGGGA GGATACAGAG 
AAGCACAAGG GGAGACCAGC CGTGCCTGAA AAACAGGAAA GTGAACTGTC TCAAGGGAAG 
CCCAAGAGCA AAAAGCTCTT AAACGCTAAG AAAGAAATCC CAACAGATGT GAAAGGCAGT 
TCCTTTGAAC TACCGTACAC TCCCTCACTG GAAAATAAAC AGCGTAGAAA TCTCCCAACC 
AAGATTCCTC TTCCAACTAC ACTAGCAAGT GGATCCAAAT CACGAAATCC CCCGAAAACA 
AAAGGTACAA ATAAGTTAGT GGAAAACAGG CCAGTTGCCC TCTCAAAGTT CCTCCCCAGC 
AGTCAAGAAC TAGGTAATAC CAGCAGCTCA GAGGGTGAAA AGGATTCTCC TCCACCAGAA 
TGGGATGCTG TGCCAGTTCA CAAGCCCAGC AGCTCTACTG ACAGTCTTTA TAAACTGTCT 
CTGCAAACCC TCAATGCAGA CATTTTCTTA AAACAACGCC AGACCTCGCC AACACCTGCC 
TCTCCTTCTC TGCCCACCGC TCCCTGCCCG TTCACATCTC GTGGCAGCTA CAGCAGCGTT 
GTCAACAGCT CCGGCAGGTA CCTGACCCCA GCACTGACCT TATCCTGTCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmem131 ( XM_017321814.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177303.1 Mus musculus transmembrane protein 131 (Tmem131), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu77244
Clone ID Related Accession (Same CDS sequence)	XM_017321814.2 , XM_017321814.1
Accession Version	XM_017321814.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	transmembrane protein 131 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321814.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGC GGGCGGGAGG AGCAGCCGCC GCCGCCGCCG CCGCCTCCAC GTCCTCCGCG 
GCCGGGCTGG AGCCCGCGGC TGGCCGAGGC GGGGGTCCTC GGAGCGCAGC CGCGGGCCTC 
CTGGGAGCGC TGCACCTGGT GATGACCCTC GTGGTGGCCG CGGCGCGGGC CGAGAAGGAA 
GCATTCATTC AGTCGGAGAG CATAATAGAA GTCCTGCGCT TTGACGACGG AGGGCTACTA 
CAGACTGAGA CAACACTTGG CCTCGGTTCA TATCAACAGA AAAGCATTTC TCTGTACCGG 
GGTAATTGCA GGCCCATACG ATTCGAACCG CCCATGCTGG ATTTCCATGA GCAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTCTACTTA CATAACCCTA GTTCTGAAGA AACGATTACT 
TTAGTATCAA TATCTGCTAC CACATCACAT TTCCATGCAT CGTTTTTTCA AAATAGGAAA 
ATTCTTCCAG GAGGAAACAC ATCATTTGAT GTAGTTTTTC TTGCAAGAGT AGTAGGAAAT 
GTAGAAAATA CTTTATTTAT TAACACATCT AACCATGGGG TGTTCACTTA CCAGGTATTT 
GGTGTTGGGG TTCCAAATCC GTATCGATTA AGGCCATTTC TTGGGGCCAG AGTCCCCGTA 
AACAGCAGCT TTTCACCCAT AATAAACATA CACAATCCTC ACAGTGAGCC TTTACAGGTC 
GTAGAGATGT ACTCGAGTGG AGGTGACCTT CACCTAGAGC TCCCAACAGG TCAGCAAGGA 
GGCACCAGGA AGCTGTGGGA AATCCCTCCT TATGAAACCA AGGGAGTGAT GAGAGCCAGC 
TTTTCCTCCA GAGAAGCAGA CAATCACACA GCCTTCATCC GGATAAAGAC GAATGCATCA 
GACAGCACCG AGTTCATCAT TCTCCCGGTT GAGGTTGAAG TTACAACAGC TCCTGGAATC 
TATTCCTCAA CTGAAATGTT AGATTTTGGA ACACTTAGAA CACAAGATCT ACCAAAGGTT 
TTAAATCTTC ATTTACTAAA TTCAGGAACA AAGGATGTAC CAATAACAAG TGTTCGACCA 
ACACCACAAA ATGACGCTAT CACGGTACAT TTTAAACCAG TTACGTTAAA AGCTTCTGAA 
AGTAAATATA CCAAGGTTGC CAGCATTAGT TTTGATGCTT CGAGAGCAAA AAAGCCATCT 
CAATTTTCTG GGAAAATAAC TGTTAAAGCA AAGGAAAAGA GTTATTCTAA ACTCGAAATC 
CCCTACCAAG CAGAAGTTCT AGATGGTTAT TTGGGGTTTG ATCACGCCGC CACGCTGTTT 
CACATCCAGG ACAGTCCTGC TGACCCAGTG GAGAGGCCGA TCTACCTGAC GAACACTTTC 
AGTTTTGCGA TCCTCATTCA TGATGTGCTG TTACCAGAAG AAGCCAGAAT AATGTTTCAG 
GTGCACAACT TCAGCCAGCC GGTTCTCATT CTTCCTAATG AGTCGGGATA CATTTTTACC 
CTGTTTTTTA TGCCATCCAC CTCATCCATG CACATAGACA ACAACATTTT ACTTGTTACC 
AATGCTTCTA AGTTTCATTT ACCTGTGCGG GTATACACAG GCTTTTTAGA TTACTTTGTA 
TTGCCCCCCA AAATAGAAGA ACGCTTCATA GATTTTGGAG TACTGAGTGC TACAGAAGCA 
AGTAGTATTT TATTTGCAAT TATAAACAGC AATCCAATTG AGCTGGCAAT AAAAAGTTGG 
CATATCATAG GAGATGGCTT ATCAATAGAA CTTGTAGCTA CAGAGAGAGG CAACAGAAGC 
ACGGTGATTG CAAGCCTTCC AGAACTGGAG CGGTCCTCTT TGCCCGACCA GTCCCCGGTA 
ACATTAGCTT CAGGACACTT TGCAGTGTTC AGAGTCAAAC TTACTGCCAA GAAGCTAGAA 
GGGGTCCATG ATGGGGCCAT ACAGATCACA ACCGACTACG AGATCCTGAC CATCCCTGTG 
AAAGCTGTGA TCGCCGTGGG CTCGCTGACC TGCTTCCCCA AGCACATGGT TCTTCCCCCT 
TCCTTCCCAG GGAAAATAGT TCATCAGAGC TTAAATATTA TGAATTCCTT CTCACAGAAG 
GTGAAGATAC AGCAAATAAG GTCTTTGTCA GAAGACGTAC GATTCTACTA CAAACGATTA 
CGGGGTAATA GGGAAGACTT GGAGCCAGGG AAGAAGTCTA AGATTGCAAA CATTTATTTT 
GACCCTGGGC TGCAGTGTGG GGATCACTGC TATATCGGCT TGCCTTTCCT ATCCAAATCT 
GAACCCAAAG TACAGCCTGG GGTGGCCATG CAGGAGGACT TGTGGGATGC CGACTGGGAC 
GCACATCAGA GCTTGTTCAA GGCTTGGATG GGGATAAAGG AAAACGCAGG ACATAGGTTG 
AATGCTATGT TTGAAGTAAA TACAGATCTT CAGAAAAATA TCGTATCAAA AGTCAGTGCC 
GAGCTCTCCT GGCCGTCTGT CCTTAGCTCG CCCCGGCTCC TGAAGTTCCC ACTCACTAAC 
ACAAACTGTT CTTCGGAAGA AGAGATTAGC TTAGAAAACC CTGCAGACGT CCCTGTCTAC 
GTCCAGTTCA TCCCACTGGC TTTGTATTCC AACCCCTCCG TCTTTGCAGA TAAGTTAGTC 
TCAAGGTTTA ACTTGAGTAA GGTGGCCAAG CTAGATTTGA GAACACTTGA ATTCCAAGTC 
TACAGGAACA GCGCTCATCC ACTGCAAAGT CCAACAGGAT TTACAGAGGG TCTCTCTCGG 
CATTTCATTT TAAACCTCAT TTTAAAACCT GGAGAAAAGA AATCTGTCAA AGTAAAGTTC 
ACTCCACTCC ACAACAGAAC TGTGTCTTCC CTGATCATAG TCAGAAACAA CCTGACTGTG 
ATGGACGCTG TGATGGTTCA AGGTCAAGGG ACAACGGAGA ACTTGAGGGT GGCAGGCAAG 
CTTCCAGGTC CAGGAAGCTC CTTACGCTTC AAAATCACAG AAGCATTGTT AAAGGATTGC 
ATAGACCGTT TGAAACTAAG AGAACCAAAT TTCACATTGA AAAGAACATT CAAAGTAGAG 
AATACGGGAC AACTTGAAAT CCGTGTAGAA ACCATTGAGA TCAGTGGATA TGCGTGTGAG 
GGATATGGTT TTAAAGTGGT TAACTGTCAA GAGTTTGCCC TGAGTGCCAA CGCCTCCAGA 
GACATAGTCA TATTGTTTAC TCCAGATTTC ACAGCCTCCA GAGTCATTCG GGAGCTGAAG 
TTTGTGACAA GCAGTGGCTC CGAGTTTGTG TTTGTGTTGA ATGCCTCTCT TCCGTACCAC 
ATGCTAGCCG CCTGTGCAGA AGCCCTCCCT AGACCCAACT GGGAGCTCGC GCTCTACATC 
ATCATCTCCG GGGTCATGAG TGCACTCTTT CTCCTGGTCA TTGGAACAGC CTACTTGGAA 
GCTCAAGGGA TTTGGGAGCC CTTCCGAAGG CGACTCTCCT TTGAAGCCTC AAACCCGCCC 
TTTGATGTAG GAAGGCCATT TGATCTCAGG AGAATCGTTG GTATTTCATC TGAAGGAAAC 
TTGAACACAC TTGGCTGCGA ACACAGTCAT GGTAGGGGTT TCTATAGTAA CGCATCATCC 
CGGCCTGGTA CAGGCAGTCA CAGGCAGTGT GGCACATCAG TCCACCCACA CAGCAGCCAT 
GGCAGCAAAA ACTCAGCTGA TGTGGACAAC GTCAGAACCA GAAACAGTTC AAGTATGTCA 
AGCAGGACTT CTCCACAAGC AGCTGCCTCA CAGTCTACAA GCAAAACCAG CCCCCTAGTC 
TCAGAGACAG CTGCAGCAAC CCAGGGTCAC ACAGCAAGCA GGAAGTCCAG AGGGGCAAAG 
CAGGGCCAGC ACAGCAGCCA GCACCACAGC CACAGCCACA GCCCACTGGA GCAGCACTCA 
CAGCCTCCAC CACCAGTGCC TCAGCACCAG GAGCCACCAC CCGAAAGGCT GTCTCCTGCC 
CCCCTCACAC ACCCTTCCCA CCCCGAGCGA GCCAGCACCA CACGGCACAG CTCAGAGGAC 
TCCGACATCA CCAGTCTCAT AGAAGCCATG GACAAAGACT TTGACCACCA CGACTCCTCC 
CCCCTAGACG TGTTTACAGA GCAGCCTCCG TCACCGATGT CAAAAAGCAA AGGGAAAGGA 
AAGTCTCTCC AACAACGTAA GGCCAAGCCT CCGAAGAAGC AGGAGGAGAA GGAGAAGAGG 
GGGAAGGGGA AGCCGCAGGA GGATGAGCTG AAGGACGCTC TGGCCGACGA CGACAGCTCC 
TCCACCACCA CAGAGACCTC CAACCCCGAC ACAGAGCCAC TCCTCAGGGA GGATACAGAG 
AAGCACAAGG GGAGACCAGC CGTGCCTGAA AAACAGGAAA GTGAACTGTC TCAAGGGAAG 
CCCAAGAGCA AAAAGCTCTT AAACGCTAAG AAAGAAATCC CAACAGATGT GAAAGGCAGT 
TCCTTTGAAC TACCGTACAC TCCCTCACTG GAAAATAAAC AGCGTAGAAA TCTCCCAACC 
AAGATTCCTC TTCCAACTAC ACTAGCAAGT GGATCCAAAT CACGAAATCC CCCGAAAACA 
AAAGGTACAA ATAAGTTAGT GGAAAACAGG CCAGTTGCCC TCTCAAAGTT CCTCCCCAGC 
AGTCAAGAAC TAGGTAATAC CAGCAGCTCA GAGGGTGAAA AGGATTCTCC TCCACCAGAA 
TGGGATGCTG TGCCAGTTCA CAAGCCCAGC AGCTACATTT TCTTAAAACA ACGCCAGACC 
TCGCCAACAC CTGCCTCTCC TTCTCTGCCC ACCGCTCCCT GCCCGTTCAC ATCTCGTGGC 
AGCTACAGCA GCGTTGTCAA CAGCTCCGGC AGTGACACAA AAGCCAAGCA GACGAGCAGC 
AGCAAGAGCA AGCTGACAAA GGCGGCCTCG CTCCCCGGCA AGAATGGCAA CCCTACCTTC 
GCCGCCGTCG CTGCAGGCTA TGACAAGAGC CCGGGTGGGA ATGGATTTGC TAAAATTTCT 
TCAAACAAAT CAGATTTTTC CAGCAGCCTT GGCATTTCAC ACATTCCTGT TGACAGCGAT 
GGCTCTGACA GCTCGGGTTT GTGGAGTCCT GTCAGCAACC CCAACAGCCC TGACTTCACC 
CCCCTCAATT CGTTCTCAGC CTTTGGAAAT TCTTTTAATC TAACAGGTGC AGTTTTCAGC 
AAACTGTCGC GATCCTGCAG TCAGTCGTCC CAGAGAAGTT GGAATGAGTT CAACAGCGGC 
CCCTCCTACC TTTGGGATTC TCCAGCCACA GACCCCAGCC CTTCCTGGCC GGCCAGTTCC 
AGCTCCCCAA CCCACACAGC CACTTCCATC CTTGGCAACA GCAGCGGCTT GTGGTCCACC 
ACTCCATTCA GCAGCTCCAT CTGGTCCAGC AACATCAACA GCAACCTTCC TTTCTCCACT 
CCAACAAACG CGCTGTCGAG CATCAGCCTC ATGGGCACGG AAAACTCTGC CGCCGCCCAC 
ACCCCTTCCG CCTCTGGCCC AGCTGACGAC TTGGGACAGA CCTACAACCC CTGGCGGATC 
TGGAGCCCCA CAGTCGGAAG AAGAAGCTCT GACCCTTGGT CTAATTCGCA CTTCCCTCAT 
GAAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177303.1
CDS	270..5858
Translation	MGKRAGGAAAAAAAASTSSAAGLEPAAGRGGGPRSAAAGLLGALHLVMTLVVAAARAEKEAFIQSESIIEVLRFDDGGLLQTETTLGLGSYQQKSISLYRGNCRPIRFEPPMLDFHEQPVGMPKMEKVYLHNPSSEETITLVSISATTSHFHASFFQNRKILPGGNTSFDVVFLARVVGNVENTLFINTSNHGVFTYQVFGVGVPNPYRLRPFLGARVPVNSSFSPIINIHNPHSEPLQVVEMYSSGGDLHLELPTGQQGGTRKLWEIPPYETKGVMRASFSSREADNHTAFIRIKTNASDSTEFIILPVEVEVTTAPGIYSSTEMLDFGTLRTQDLPKVLNLHLLNSGTKDVPITSVRPTPQNDAITVHFKPVTLKASESKYTKVASISFDASRAKKPSQFSGKITVKAKEKSYSKLEIPYQAEVLDGYLGFDHAATLFHIQDSPADPVERPIYLTNTFSFAILIHDVLLPEEARIMFQVHNFSQPVLILPNESGYIFTLFFMPSTSSMHIDNNILLVTNASKFHLPVRVYTGFLDYFVLPPKIEERFIDFGVLSATEASSILFAIINSNPIELAIKSWHIIGDGLSIELVATERGNRSTVIASLPELERSSLPDQSPVTLASGHFAVFRVKLTAKKLEGVHDGAIQITTDYEILTIPVKAVIAVGSLTCFPKHMVLPPSFPGKIVHQSLNIMNSFSQKVKIQQIRSLSEDVRFYYKRLRGNREDLEPGKKSKIANIYFDPGLQCGDHCYIGLPFLSKSEPKVQPGVAMQEDLWDADWDAHQSLFKAWMGIKENAGHRLNAMFEVNTDLQKNIVSKVSAELSWPSVLSSPRLLKFPLTNTNCSSEEEISLENPADVPVYVQFIPLALYSNPSVFADKLVSRFNLSKVAKLDLRTLEFQVYRNSAHPLQSPTGFTEGLSRHFILNLILKPGEKKSVKVKFTPLHNRTVSSLIIVRNNLTVMDAVMVQGQGTTENLRVAGKLPGPGSSLRFKITEALLKDCIDRLKLREPNFTLKRTFKVENTGQLEIRVETIEISGYACEGYGFKVVNCQEFALSANASRDIVILFTPDFTASRVIRELKFVTSSGSEFVFVLNASLPYHMLAACAEALPRPNWELALYIIISGVMSALFLLVIGTAYLEAQGIWEPFRRRLSFEASNPPFDVGRPFDLRRIVGISSEGNLNTLGCEHSHGRGFYSNASSRPGTGSHRQCGTSVHPHSSHGSKNSADVDNVRTRNSSSMSSRTSPQAAASQSTSKTSPLVSETAAATQGHTASRKSRGAKQGQHSSQHHSHSHSPLEQHSQPPPPVPQHQEPPPERLSPAPLTHPSHPERASTTRHSSEDSDITSLIEAMDKDFDHHDSSPLDVFTEQPPSPMSKSKGKGKSLQQRKAKPPKKQEEKEKRGKGKPQEDELKDALADDDSSSTTTETSNPDTEPLLREDTEKHKGRPAVPEKQESELSQGKPKSKKLLNAKKEIPTDVKGSSFELPYTPSLENKQRRNLPTKIPLPTTLASGSKSRNPPKTKGTNKLVENRPVALSKFLPSSQELGNTSSSEGEKDSPPPEWDAVPVHKPSSYIFLKQRQTSPTPASPSLPTAPCPFTSRGSYSSVVNSSGSDTKAKQTSSSKSKLTKAASLPGKNGNPTFAAVAAGYDKSPGGNGFAKISSNKSDFSSSLGISHIPVDSDGSDSSGLWSPVSNPNSPDFTPLNSFSAFGNSFNLTGAVFSKLSRSCSQSSQRSWNEFNSGPSYLWDSPATDPSPSWPASSSSPTHTATSILGNSSGLWSTTPFSSSIWSSNINSNLPFSTPTNALSSISLMGTENSAAAHTPSASGPADDLGQTYNPWRIWSPTVGRRSSDPWSNSHFPHEN

Target ORF information:

RefSeq Version	XM_017321814.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane protein 131 (Tmem131), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321814.2

ORF Insert Sequence:

ATGGGGAAGC GGGCGGGAGG AGCAGCCGCC GCCGCCGCCG CCGCCTCCAC GTCCTCCGCG 
GCCGGGCTGG AGCCCGCGGC TGGCCGAGGC GGGGGTCCTC GGAGCGCAGC CGCGGGCCTC 
CTGGGAGCGC TGCACCTGGT GATGACCCTC GTGGTGGCCG CGGCGCGGGC CGAGAAGGAA 
GCATTCATTC AGTCGGAGAG CATAATAGAA GTCCTGCGCT TTGACGACGG AGGGCTACTA 
CAGACTGAGA CAACACTTGG CCTCGGTTCA TATCAACAGA AAAGCATTTC TCTGTACCGG 
GGTAATTGCA GGCCCATACG ATTCGAACCG CCCATGCTGG ATTTCCATGA GCAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTCTACTTA CATAACCCTA GTTCTGAAGA AACGATTACT 
TTAGTATCAA TATCTGCTAC CACATCACAT TTCCATGCAT CGTTTTTTCA AAATAGGAAA 
ATTCTTCCAG GAGGAAACAC ATCATTTGAT GTAGTTTTTC TTGCAAGAGT AGTAGGAAAT 
GTAGAAAATA CTTTATTTAT TAACACATCT AACCATGGGG TGTTCACTTA CCAGGTATTT 
GGTGTTGGGG TTCCAAATCC GTATCGATTA AGGCCATTTC TTGGGGCCAG AGTCCCCGTA 
AACAGCAGCT TTTCACCCAT AATAAACATA CACAATCCTC ACAGTGAGCC TTTACAGGTC 
GTAGAGATGT ACTCGAGTGG AGGTGACCTT CACCTAGAGC TCCCAACAGG TCAGCAAGGA 
GGCACCAGGA AGCTGTGGGA AATCCCTCCT TATGAAACCA AGGGAGTGAT GAGAGCCAGC 
TTTTCCTCCA GAGAAGCAGA CAATCACACA GCCTTCATCC GGATAAAGAC GAATGCATCA 
GACAGCACCG AGTTCATCAT TCTCCCGGTT GAGGTTGAAG TTACAACAGC TCCTGGAATC 
TATTCCTCAA CTGAAATGTT AGATTTTGGA ACACTTAGAA CACAAGATCT ACCAAAGGTT 
TTAAATCTTC ATTTACTAAA TTCAGGAACA AAGGATGTAC CAATAACAAG TGTTCGACCA 
ACACCACAAA ATGACGCTAT CACGGTACAT TTTAAACCAG TTACGTTAAA AGCTTCTGAA 
AGTAAATATA CCAAGGTTGC CAGCATTAGT TTTGATGCTT CGAGAGCAAA AAAGCCATCT 
CAATTTTCTG GGAAAATAAC TGTTAAAGCA AAGGAAAAGA GTTATTCTAA ACTCGAAATC 
CCCTACCAAG CAGAAGTTCT AGATGGTTAT TTGGGGTTTG ATCACGCCGC CACGCTGTTT 
CACATCCAGG ACAGTCCTGC TGACCCAGTG GAGAGGCCGA TCTACCTGAC GAACACTTTC 
AGTTTTGCGA TCCTCATTCA TGATGTGCTG TTACCAGAAG AAGCCAGAAT AATGTTTCAG 
GTGCACAACT TCAGCCAGCC GGTTCTCATT CTTCCTAATG AGTCGGGATA CATTTTTACC 
CTGTTTTTTA TGCCATCCAC CTCATCCATG CACATAGACA ACAACATTTT ACTTGTTACC 
AATGCTTCTA AGTTTCATTT ACCTGTGCGG GTATACACAG GCTTTTTAGA TTACTTTGTA 
TTGCCCCCCA AAATAGAAGA ACGCTTCATA GATTTTGGAG TACTGAGTGC TACAGAAGCA 
AGTAGTATTT TATTTGCAAT TATAAACAGC AATCCAATTG AGCTGGCAAT AAAAAGTTGG 
CATATCATAG GAGATGGCTT ATCAATAGAA CTTGTAGCTA CAGAGAGAGG CAACAGAAGC 
ACGGTGATTG CAAGCCTTCC AGAACTGGAG CGGTCCTCTT TGCCCGACCA GTCCCCGGTA 
ACATTAGCTT CAGGACACTT TGCAGTGTTC AGAGTCAAAC TTACTGCCAA GAAGCTAGAA 
GGGGTCCATG ATGGGGCCAT ACAGATCACA ACCGACTACG AGATCCTGAC CATCCCTGTG 
AAAGCTGTGA TCGCCGTGGG CTCGCTGACC TGCTTCCCCA AGCACATGGT TCTTCCCCCT 
TCCTTCCCAG GGAAAATAGT TCATCAGAGC TTAAATATTA TGAATTCCTT CTCACAGAAG 
GTGAAGATAC AGCAAATAAG GTCTTTGTCA GAAGACGTAC GATTCTACTA CAAACGATTA 
CGGGGTAATA GGGAAGACTT GGAGCCAGGG AAGAAGTCTA AGATTGCAAA CATTTATTTT 
GACCCTGGGC TGCAGTGTGG GGATCACTGC TATATCGGCT TGCCTTTCCT ATCCAAATCT 
GAACCCAAAG TACAGCCTGG GGTGGCCATG CAGGAGGACT TGTGGGATGC CGACTGGGAC 
GCACATCAGA GCTTGTTCAA GGCTTGGATG GGGATAAAGG AAAACGCAGG ACATAGGTTG 
AATGCTATGT TTGAAGTAAA TACAGATCTT CAGAAAAATA TCGTATCAAA AGTCAGTGCC 
GAGCTCTCCT GGCCGTCTGT CCTTAGCTCG CCCCGGCTCC TGAAGTTCCC ACTCACTAAC 
ACAAACTGTT CTTCGGAAGA AGAGATTAGC TTAGAAAACC CTGCAGACGT CCCTGTCTAC 
GTCCAGTTCA TCCCACTGGC TTTGTATTCC AACCCCTCCG TCTTTGCAGA TAAGTTAGTC 
TCAAGGTTTA ACTTGAGTAA GGTGGCCAAG CTAGATTTGA GAACACTTGA ATTCCAAGTC 
TACAGGAACA GCGCTCATCC ACTGCAAAGT CCAACAGGAT TTACAGAGGG TCTCTCTCGG 
CATTTCATTT TAAACCTCAT TTTAAAACCT GGAGAAAAGA AATCTGTCAA AGTAAAGTTC 
ACTCCACTCC ACAACAGAAC TGTGTCTTCC CTGATCATAG TCAGAAACAA CCTGACTGTG 
ATGGACGCTG TGATGGTTCA AGGTCAAGGG ACAACGGAGA ACTTGAGGGT GGCAGGCAAG 
CTTCCAGGTC CAGGAAGCTC CTTACGCTTC AAAATCACAG AAGCATTGTT AAAGGATTGC 
ATAGACCGTT TGAAACTAAG AGAACCAAAT TTCACATTGA AAAGAACATT CAAAGTAGAG 
AATACGGGAC AACTTGAAAT CCGTGTAGAA ACCATTGAGA TCAGTGGATA TGCGTGTGAG 
GGATATGGTT TTAAAGTGGT TAACTGTCAA GAGTTTGCCC TGAGTGCCAA CGCCTCCAGA 
GACATAGTCA TATTGTTTAC TCCAGATTTC ACAGCCTCCA GAGTCATTCG GGAGCTGAAG 
TTTGTGACAA GCAGTGGCTC CGAGTTTGTG TTTGTGTTGA ATGCCTCTCT TCCGTACCAC 
ATGCTAGCCG CCTGTGCAGA AGCCCTCCCT AGACCCAACT GGGAGCTCGC GCTCTACATC 
ATCATCTCCG GGGTCATGAG TGCACTCTTT CTCCTGGTCA TTGGAACAGC CTACTTGGAA 
GCTCAAGGGA TTTGGGAGCC CTTCCGAAGG CGACTCTCCT TTGAAGCCTC AAACCCGCCC 
TTTGATGTAG GAAGGCCATT TGATCTCAGG AGAATCGTTG GTATTTCATC TGAAGGAAAC 
TTGAACACAC TTGGCTGCGA ACACAGTCAT GGTAGGGGTT TCTATAGTAA CGCATCATCC 
CGGCCTGGTA CAGGCAGTCA CAGGCAGTGT GGCACATCAG TCCACCCACA CAGCAGCCAT 
GGCAGCAAAA ACTCAGCTGA TGTGGACAAC GTCAGAACCA GAAACAGTTC AAGTATGTCA 
AGCAGGACTT CTCCACAAGC AGCTGCCTCA CAGTCTACAA GCAAAACCAG CCCCCTAGTC 
TCAGAGACAG CTGCAGCAAC CCAGGGTCAC ACAGCAAGCA GGAAGTCCAG AGGGGCAAAG 
CAGGGCCAGC ACAGCAGCCA GCACCACAGC CACAGCCACA GCCCACTGGA GCAGCACTCA 
CAGCCTCCAC CACCAGTGCC TCAGCACCAG GAGCCACCAC CCGAAAGGCT GTCTCCTGCC 
CCCCTCACAC ACCCTTCCCA CCCCGAGCGA GCCAGCACCA CACGGCACAG CTCAGAGGAC 
TCCGACATCA CCAGTCTCAT AGAAGCCATG GACAAAGACT TTGACCACCA CGACTCCTCC 
CCCCTAGACG TGTTTACAGA GCAGCCTCCG TCACCGATGT CAAAAAGCAA AGGGAAAGGA 
AAGTCTCTCC AACAACGTAA GGCCAAGCCT CCGAAGAAGC AGGAGGAGAA GGAGAAGAGG 
GGGAAGGGGA AGCCGCAGGA GGATGAGCTG AAGGACGCTC TGGCCGACGA CGACAGCTCC 
TCCACCACCA CAGAGACCTC CAACCCCGAC ACAGAGCCAC TCCTCAGGGA GGATACAGAG 
AAGCACAAGG GGAGACCAGC CGTGCCTGAA AAACAGGAAA GTGAACTGTC TCAAGGGAAG 
CCCAAGAGCA AAAAGCTCTT AAACGCTAAG AAAGAAATCC CAACAGATGT GAAAGGCAGT 
TCCTTTGAAC TACCGTACAC TCCCTCACTG GAAAATAAAC AGCGTAGAAA TCTCCCAACC 
AAGATTCCTC TTCCAACTAC ACTAGCAAGT GGATCCAAAT CACGAAATCC CCCGAAAACA 
AAAGGTACAA ATAAGTTAGT GGAAAACAGG CCAGTTGCCC TCTCAAAGTT CCTCCCCAGC 
AGTCAAGAAC TAGGTAATAC CAGCAGCTCA GAGGGTGAAA AGGATTCTCC TCCACCAGAA 
TGGGATGCTG TGCCAGTTCA CAAGCCCAGC AGCTACATTT TCTTAAAACA ACGCCAGACC 
TCGCCAACAC CTGCCTCTCC TTCTCTGCCC ACCGCTCCCT GCCCGTTCAC ATCTCGTGGC 
AGCTACAGCA GCGTTGTCAA CAGCTCCGGC AGTGACACAA AAGCCAAGCA GACGAGCAGC 
AGCAAGAGCA AGCTGACAAA GGCGGCCTCG CTCCCCGGCA AGAATGGCAA CCCTACCTTC 
GCCGCCGTCG CTGCAGGCTA TGACAAGAGC CCGGGTGGGA ATGGATTTGC TAAAATTTCT 
TCAAACAAAT CAGATTTTTC CAGCAGCCTT GGCATTTCAC ACATTCCTGT TGACAGCGAT 
GGCTCTGACA GCTCGGGTTT GTGGAGTCCT GTCAGCAACC CCAACAGCCC TGACTTCACC 
CCCCTCAATT CGTTCTCAGC CTTTGGAAAT TCTTTTAATC TAACAGGTGC AGTTTTCAGC 
AAACTGTCGC GATCCTGCAG TCAGTCGTCC CAGAGAAGTT GGAATGAGTT CAACAGCGGC 
CCCTCCTACC TTTGGGATTC TCCAGCCACA GACCCCAGCC CTTCCTGGCC GGCCAGTTCC 
AGCTCCCCAA CCCACACAGC CACTTCCATC CTTGGCAACA GCAGCGGCTT GTGGTCCACC 
ACTCCATTCA GCAGCTCCAT CTGGTCCAGC AACATCAACA GCAACCTTCC TTTCTCCACT 
CCAACAAACG CGCTGTCGAG CATCAGCCTC ATGGGCACGG AAAACTCTGC CGCCGCCCAC 
ACCCCTTCCG CCTCTGGCCC AGCTGACGAC TTGGGACAGA CCTACAACCC CTGGCGGATC 
TGGAGCCCCA CAGTCGGAAG AAGAAGCTCT GACCCTTGGT CTAATTCGCA CTTCCCTCAT 
GAAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmem131 ( XM_011238561.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236863.1 Mus musculus transmembrane protein 131 (Tmem131), transcript variant X3, mRNA.

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CloneID	OMu27535
Clone ID Related Accession (Same CDS sequence)	XM_011238561.2
Accession Version	XM_011238561.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	transmembrane protein 131 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238561.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCTCG TGGTGGCCGC GGCGCGGGCC GAGAAGGAAG CATTCATTCA GTCGGAGAGC 
ATAATAGAAG TCCTGCGCTT TGACGACGGA GGGCTACTAC AGACTGAGAC AACACTTGGC 
CTCGGTTCAT ATCAACAGAA AAGCATTTCT CTGTACCGGG GTAATTGCAG GCCCATACGA 
TTCGAACCGC CCATGCTGGA TTTCCATGAG CAGCCAGTTG GAATGCCAAA AATGGAAAAA 
GTCTACTTAC ATAACCCTAG TTCTGAAGAA ACGATTACTT TAGTATCAAT ATCTGCTACC 
ACATCACATT TCCATGCATC GTTTTTTCAA AATAGGAAAA TTCTTCCAGG AGGAAACACA 
TCATTTGATG TAGTTTTTCT TGCAAGAGTA GTAGGAAATG TAGAAAATAC TTTATTTATT 
AACACATCTA ACCATGGGGT GTTCACTTAC CAGGTATTTG GTGTTGGGGT TCCAAATCCG 
TATCGATTAA GGCCATTTCT TGGGGCCAGA GTCCCCGTAA ACAGCAGCTT TTCACCCATA 
ATAAACATAC ACAATCCTCA CAGTGAGCCT TTACAGGTCG TAGAGATGTA CTCGAGTGGA 
GGTGACCTTC ACCTAGAGCT CCCAACAGGT CAGCAAGGAG GCACCAGGAA GCTGTGGGAA 
ATCCCTCCTT ATGAAACCAA GGGAGTGATG AGAGCCAGCT TTTCCTCCAG AGAAGCAGAC 
AATCACACAG CCTTCATCCG GATAAAGACG AATGCATCAG ACAGCACCGA GTTCATCATT 
CTCCCGGTTG AGGTTGAAGT TACAACAGCT CCTGGAATCT ATTCCTCAAC TGAAATGTTA 
GATTTTGGAA CACTTAGAAC ACAAGATCTA CCAAAGGTTT TAAATCTTCA TTTACTAAAT 
TCAGGAACAA AGGATGTACC AATAACAAGT GTTCGACCAA CACCACAAAA TGACGCTATC 
ACGGTACATT TTAAACCAGT TACGTTAAAA GCTTCTGAAA GTAAATATAC CAAGGTTGCC 
AGCATTAGTT TTGATGCTTC GAGAGCAAAA AAGCCATCTC AATTTTCTGG GAAAATAACT 
GTTAAAGCAA AGGAAAAGAG TTATTCTAAA CTCGAAATCC CCTACCAAGC AGAAGTTCTA 
GATGGTTATT TGGGGTTTGA TCACGCCGCC ACGCTGTTTC ACATCCAGGA CAGTCCTGCT 
GACCCAGTGG AGAGGCCGAT CTACCTGACG AACACTTTCA GTTTTGCGAT CCTCATTCAT 
GATGTGCTGT TACCAGAAGA AGCCAGAATA ATGTTTCAGG TGCACAACTT CAGCCAGCCG 
GTTCTCATTC TTCCTAATGA GTCGGGATAC ATTTTTACCC TGTTTTTTAT GCCATCCACC 
TCATCCATGC ACATAGACAA CAACATTTTA CTTGTTACCA ATGCTTCTAA GTTTCATTTA 
CCTGTGCGGG TATACACAGG CTTTTTAGAT TACTTTGTAT TGCCCCCCAA AATAGAAGAA 
CGCTTCATAG ATTTTGGAGT ACTGAGTGCT ACAGAAGCAA GTAGTATTTT ATTTGCAATT 
ATAAACAGCA ATCCAATTGA GCTGGCAATA AAAAGTTGGC ATATCATAGG AGATGGCTTA 
TCAATAGAAC TTGTAGCTAC AGAGAGAGGC AACAGAAGCA CGGTGATTGC AAGCCTTCCA 
GAACTGGAGC GGTCCTCTTT GCCCGACCAG TCCCCGGTAA CATTAGCTTC AGGACACTTT 
GCAGTGTTCA GAGTCAAACT TACTGCCAAG AAGCTAGAAG GGGTCCATGA TGGGGCCATA 
CAGATCACAA CCGACTACGA GATCCTGACC ATCCCTGTGA AAGCTGTGAT CGCCGTGGGC 
TCGCTGACCT GCTTCCCCAA GCACATGGTT CTTCCCCCTT CCTTCCCAGG GAAAATAGTT 
CATCAGAGCT TAAATATTAT GAATTCCTTC TCACAGAAGG TGAAGATACA GCAAATAAGG 
TCTTTGTCAG AAGACGTACG ATTCTACTAC AAACGATTAC GGGGTAATAG GGAAGACTTG 
GAGCCAGGGA AGAAGTCTAA GATTGCAAAC ATTTATTTTG ACCCTGGGCT GCAGTGTGGG 
GATCACTGCT ATATCGGCTT GCCTTTCCTA TCCAAATCTG AACCCAAAGT ACAGCCTGGG 
GTGGCCATGC AGGAGGACTT GTGGGATGCC GACTGGGACG CACATCAGAG CTTGTTCAAG 
GCTTGGATGG GGATAAAGGA AAACGCAGGA CATAGGTTGA ATGCTATGTT TGAAGTAAAT 
ACAGATCTTC AGAAAAATAT CGTATCAAAA GTCAGTGCCG AGCTCTCCTG GCCGTCTGTC 
CTTAGCTCGC CCCGGCTCCT GAAGTTCCCA CTCACTAACA CAAACTGTTC TTCGGAAGAA 
GAGATTAGCT TAGAAAACCC TGCAGACGTC CCTGTCTACG TCCAGTTCAT CCCACTGGCT 
TTGTATTCCA ACCCCTCCGT CTTTGCAGAT AAGTTAGTCT CAAGGTTTAA CTTGAGTAAG 
GTGGCCAAGC TAGATTTGAG AACACTTGAA TTCCAAGTCT ACAGGAACAG CGCTCATCCA 
CTGCAAAGTC CAACAGGATT TACAGAGGGT CTCTCTCGGC ATTTCATTTT AAACCTCATT 
TTAAAACCTG GAGAAAAGAA ATCTGTCAAA GTAAAGTTCA CTCCACTCCA CAACAGAACT 
GTGTCTTCCC TGATCATAGT CAGAAACAAC CTGACTGTGA TGGACGCTGT GATGGTTCAA 
GGTCAAGGGA CAACGGAGAA CTTGAGGGTG GCAGGCAAGC TTCCAGGTCC AGGAAGCTCC 
TTACGCTTCA AAATCACAGA AGCATTGTTA AAGGATTGCA TAGACCGTTT GAAACTAAGA 
GAACCAAATT TCACATTGAA AAGAACATTC AAAGTAGAGA ATACGGGACA ACTTGAAATC 
CGTGTAGAAA CCATTGAGAT CAGTGGATAT GCGTGTGAGG GATATGGTTT TAAAGTGGTT 
AACTGTCAAG AGTTTGCCCT GAGTGCCAAC GCCTCCAGAG ACATAGTCAT ATTGTTTACT 
CCAGATTTCA CAGCCTCCAG AGTCATTCGG GAGCTGAAGT TTGTGACAAG CAGTGGCTCC 
GAGTTTGTGT TTGTGTTGAA TGCCTCTCTT CCGTACCACA TGCTAGCCGC CTGTGCAGAA 
GCCCTCCCTA GACCCAACTG GGAGCTCGCG CTCTACATCA TCATCTCCGG GGTCATGAGT 
GCACTCTTTC TCCTGGTCAT TGGAACAGCC TACTTGGAAG CTCAAGGGAT TTGGGAGCCC 
TTCCGAAGGC GACTCTCCTT TGAAGCCTCA AACCCGCCCT TTGATGTAGG AAGGCCATTT 
GATCTCAGGA GAATCGTTGG TATTTCATCT GAAGGAAACT TGAACACACT TGGCTGCGAA 
CACAGTCATG GTAGGGGTTT CTATAGTAAC GCATCATCCC GGCCTGGTAC AGGCAGTCAC 
AGGCAGTGTG GCACATCAGT CCACCCACAC AGCAGCCATG GCAGCAAAAA CTCAGCTGAT 
GTGGACAACG TCAGAACCAG AAACAGTTCA AGTATGTCAA GCAGGACTTC TCCACAAGCA 
GCTGCCTCAC AGTCTACAAG CAAAACCAGC CCCCTAGTCT CAGAGACAGC TGCAGCAACC 
CAGGGTCACA CAGCAAGCAG GAAGTCCAGA GGGGCAAAGC AGGGCCAGCA CAGCAGCCAG 
CACCACAGCC ACAGCCACAG CCCACTGGAG CAGCACTCAC AGCCTCCACC ACCAGTGCCT 
CAGCACCAGG AGCCACCACC CGAAAGGCTG TCTCCTGCCC CCCTCACACA CCCTTCCCAC 
CCCGAGCGAG CCAGCACCAC ACGGCACAGC TCAGAGGACT CCGACATCAC CAGTCTCATA 
GAAGCCATGG ACAAAGACTT TGACCACCAC GACTCCTCCC CCCTAGACGT GTTTACAGAG 
CAGCCTCCGT CACCGATGTC AAAAAGCAAA GGGAAAGGAA AGTCTCTCCA ACAACGTAAG 
GCCAAGCCTC CGAAGAAGCA GGAGGAGAAG GAGAAGAGGG GGAAGGGGAA GCCGCAGGAG 
GATGAGCTGA AGGACGCTCT GGCCGACGAC GACAGCTCCT CCACCACCAC AGAGACCTCC 
AACCCCGACA CAGAGCCACT CCTCAGGGAG GATACAGAGA AGCACAAGGG GAGACCAGCC 
GTGCCTGAAA AACAGGAAAG TGAACTGTCT CAAGGGAAGC CCAAGAGCAA AAAGCTCTTA 
AACGCTAAGA AAGAAATCCC AACAGATGTG AAAGGCAGTT CCTTTGAACT ACCGTACACT 
CCCTCACTGG AAAATAAACA GCGTAGAAAT CTCCCAACCA AGATTCCTCT TCCAACTACA 
CTAGCAAGTG GATCCAAATC ACGAAATCCC CCGAAAACAA AAGGTACAAA TAAGTTAGTG 
GAAAACAGGC CAGTTGCCCT CTCAAAGTTC CTCCCCAGCA GTCAAGAACT AGGTAATACC 
AGCAGCTCAG AGGGTGAAAA GGATTCTCCT CCACCAGAAT GGGATGCTGT GCCAGTTCAC 
AAGCCCAGCA GCTCTACTGA CAGTCTTTAT AAACTGTCTC TGCAAACCCT CAATGCAGAC 
ATTTTCTTAA AACAACGCCA GACCTCGCCA ACACCTGCCT CTCCTTCTCT GCCCACCGCT 
CCCTGCCCGT TCACATCTCG TGGCAGCTAC AGCAGCGTTG TCAACAGCTC CGGCAGTGAC 
ACAAAAGCCA AGCAGACGAG CAGCAGCAAG AGCAAGCTGA CAAAGGCGGC CTCGCTCCCC 
GGCAAGAATG GCAACCCTAC CTTCGCCGCC GTCGCTGCAG GCTATGACAA GAGCCCGGGT 
GGGAATGGAT TTGCTAAAAT TTCTTCAAAC AAATCAGATT TTTCCAGCAG CCTTGGCATT 
TCACACATTC CTGTTGACAG CGATGGCTCT GACAGCTCGG GTTTGTGGAG TCCTGTCAGC 
AACCCCAACA GCCCTGACTT CACCCCCCTC AATTCGTTCT CAGCCTTTGG AAATTCTTTT 
AATCTAACAG GTGCAGTTTT CAGCAAACTG TCGCGATCCT GCAGTCAGTC GTCCCAGAGA 
AGTTGGAATG AGTTCAACAG CGGCCCCTCC TACCTTTGGG ATTCTCCAGC CACAGACCCC 
AGCCCTTCCT GGCCGGCCAG TTCCAGCTCC CCAACCCACA CAGCCACTTC CATCCTTGGC 
AACAGCAGCG GCTTGTGGTC CACCACTCCA TTCAGCAGCT CCATCTGGTC CAGCAACATC 
AACAGCAACC TTCCTTTCTC CACTCCAACA AACGCGCTGT CGAGCATCAG CCTCATGGGC 
ACGGAAAACT CTGCCGCCGC CCACACCCCT TCCGCCTCTG GCCCAGCTGA CGACTTGGGA 
CAGACCTACA ACCCCTGGCG GATCTGGAGC CCCACAGTCG GAAGAAGAAG CTCTGACCCT 
TGGTCTAATT CGCACTTCCC TCATGAAAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236863.1
CDS	598..6090
Translation	MTLVVAAARAEKEAFIQSESIIEVLRFDDGGLLQTETTLGLGSYQQKSISLYRGNCRPIRFEPPMLDFHEQPVGMPKMEKVYLHNPSSEETITLVSISATTSHFHASFFQNRKILPGGNTSFDVVFLARVVGNVENTLFINTSNHGVFTYQVFGVGVPNPYRLRPFLGARVPVNSSFSPIINIHNPHSEPLQVVEMYSSGGDLHLELPTGQQGGTRKLWEIPPYETKGVMRASFSSREADNHTAFIRIKTNASDSTEFIILPVEVEVTTAPGIYSSTEMLDFGTLRTQDLPKVLNLHLLNSGTKDVPITSVRPTPQNDAITVHFKPVTLKASESKYTKVASISFDASRAKKPSQFSGKITVKAKEKSYSKLEIPYQAEVLDGYLGFDHAATLFHIQDSPADPVERPIYLTNTFSFAILIHDVLLPEEARIMFQVHNFSQPVLILPNESGYIFTLFFMPSTSSMHIDNNILLVTNASKFHLPVRVYTGFLDYFVLPPKIEERFIDFGVLSATEASSILFAIINSNPIELAIKSWHIIGDGLSIELVATERGNRSTVIASLPELERSSLPDQSPVTLASGHFAVFRVKLTAKKLEGVHDGAIQITTDYEILTIPVKAVIAVGSLTCFPKHMVLPPSFPGKIVHQSLNIMNSFSQKVKIQQIRSLSEDVRFYYKRLRGNREDLEPGKKSKIANIYFDPGLQCGDHCYIGLPFLSKSEPKVQPGVAMQEDLWDADWDAHQSLFKAWMGIKENAGHRLNAMFEVNTDLQKNIVSKVSAELSWPSVLSSPRLLKFPLTNTNCSSEEEISLENPADVPVYVQFIPLALYSNPSVFADKLVSRFNLSKVAKLDLRTLEFQVYRNSAHPLQSPTGFTEGLSRHFILNLILKPGEKKSVKVKFTPLHNRTVSSLIIVRNNLTVMDAVMVQGQGTTENLRVAGKLPGPGSSLRFKITEALLKDCIDRLKLREPNFTLKRTFKVENTGQLEIRVETIEISGYACEGYGFKVVNCQEFALSANASRDIVILFTPDFTASRVIRELKFVTSSGSEFVFVLNASLPYHMLAACAEALPRPNWELALYIIISGVMSALFLLVIGTAYLEAQGIWEPFRRRLSFEASNPPFDVGRPFDLRRIVGISSEGNLNTLGCEHSHGRGFYSNASSRPGTGSHRQCGTSVHPHSSHGSKNSADVDNVRTRNSSSMSSRTSPQAAASQSTSKTSPLVSETAAATQGHTASRKSRGAKQGQHSSQHHSHSHSPLEQHSQPPPPVPQHQEPPPERLSPAPLTHPSHPERASTTRHSSEDSDITSLIEAMDKDFDHHDSSPLDVFTEQPPSPMSKSKGKGKSLQQRKAKPPKKQEEKEKRGKGKPQEDELKDALADDDSSSTTTETSNPDTEPLLREDTEKHKGRPAVPEKQESELSQGKPKSKKLLNAKKEIPTDVKGSSFELPYTPSLENKQRRNLPTKIPLPTTLASGSKSRNPPKTKGTNKLVENRPVALSKFLPSSQELGNTSSSEGEKDSPPPEWDAVPVHKPSSSTDSLYKLSLQTLNADIFLKQRQTSPTPASPSLPTAPCPFTSRGSYSSVVNSSGSDTKAKQTSSSKSKLTKAASLPGKNGNPTFAAVAAGYDKSPGGNGFAKISSNKSDFSSSLGISHIPVDSDGSDSSGLWSPVSNPNSPDFTPLNSFSAFGNSFNLTGAVFSKLSRSCSQSSQRSWNEFNSGPSYLWDSPATDPSPSWPASSSSPTHTATSILGNSSGLWSTTPFSSSIWSSNINSNLPFSTPTNALSSISLMGTENSAAAHTPSASGPADDLGQTYNPWRIWSPTVGRRSSDPWSNSHFPHEN

Target ORF information:

RefSeq Version	XM_011238561.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane protein 131 (Tmem131), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238561.2

ORF Insert Sequence:

ATGACCCTCG TGGTGGCCGC GGCGCGGGCC GAGAAGGAAG CATTCATTCA GTCGGAGAGC 
ATAATAGAAG TCCTGCGCTT TGACGACGGA GGGCTACTAC AGACTGAGAC AACACTTGGC 
CTCGGTTCAT ATCAACAGAA AAGCATTTCT CTGTACCGGG GTAATTGCAG GCCCATACGA 
TTCGAACCGC CCATGCTGGA TTTCCATGAG CAGCCAGTTG GAATGCCAAA AATGGAAAAA 
GTCTACTTAC ATAACCCTAG TTCTGAAGAA ACGATTACTT TAGTATCAAT ATCTGCTACC 
ACATCACATT TCCATGCATC GTTTTTTCAA AATAGGAAAA TTCTTCCAGG AGGAAACACA 
TCATTTGATG TAGTTTTTCT TGCAAGAGTA GTAGGAAATG TAGAAAATAC TTTATTTATT 
AACACATCTA ACCATGGGGT GTTCACTTAC CAGGTATTTG GTGTTGGGGT TCCAAATCCG 
TATCGATTAA GGCCATTTCT TGGGGCCAGA GTCCCCGTAA ACAGCAGCTT TTCACCCATA 
ATAAACATAC ACAATCCTCA CAGTGAGCCT TTACAGGTCG TAGAGATGTA CTCGAGTGGA 
GGTGACCTTC ACCTAGAGCT CCCAACAGGT CAGCAAGGAG GCACCAGGAA GCTGTGGGAA 
ATCCCTCCTT ATGAAACCAA GGGAGTGATG AGAGCCAGCT TTTCCTCCAG AGAAGCAGAC 
AATCACACAG CCTTCATCCG GATAAAGACG AATGCATCAG ACAGCACCGA GTTCATCATT 
CTCCCGGTTG AGGTTGAAGT TACAACAGCT CCTGGAATCT ATTCCTCAAC TGAAATGTTA 
GATTTTGGAA CACTTAGAAC ACAAGATCTA CCAAAGGTTT TAAATCTTCA TTTACTAAAT 
TCAGGAACAA AGGATGTACC AATAACAAGT GTTCGACCAA CACCACAAAA TGACGCTATC 
ACGGTACATT TTAAACCAGT TACGTTAAAA GCTTCTGAAA GTAAATATAC CAAGGTTGCC 
AGCATTAGTT TTGATGCTTC GAGAGCAAAA AAGCCATCTC AATTTTCTGG GAAAATAACT 
GTTAAAGCAA AGGAAAAGAG TTATTCTAAA CTCGAAATCC CCTACCAAGC AGAAGTTCTA 
GATGGTTATT TGGGGTTTGA TCACGCCGCC ACGCTGTTTC ACATCCAGGA CAGTCCTGCT 
GACCCAGTGG AGAGGCCGAT CTACCTGACG AACACTTTCA GTTTTGCGAT CCTCATTCAT 
GATGTGCTGT TACCAGAAGA AGCCAGAATA ATGTTTCAGG TGCACAACTT CAGCCAGCCG 
GTTCTCATTC TTCCTAATGA GTCGGGATAC ATTTTTACCC TGTTTTTTAT GCCATCCACC 
TCATCCATGC ACATAGACAA CAACATTTTA CTTGTTACCA ATGCTTCTAA GTTTCATTTA 
CCTGTGCGGG TATACACAGG CTTTTTAGAT TACTTTGTAT TGCCCCCCAA AATAGAAGAA 
CGCTTCATAG ATTTTGGAGT ACTGAGTGCT ACAGAAGCAA GTAGTATTTT ATTTGCAATT 
ATAAACAGCA ATCCAATTGA GCTGGCAATA AAAAGTTGGC ATATCATAGG AGATGGCTTA 
TCAATAGAAC TTGTAGCTAC AGAGAGAGGC AACAGAAGCA CGGTGATTGC AAGCCTTCCA 
GAACTGGAGC GGTCCTCTTT GCCCGACCAG TCCCCGGTAA CATTAGCTTC AGGACACTTT 
GCAGTGTTCA GAGTCAAACT TACTGCCAAG AAGCTAGAAG GGGTCCATGA TGGGGCCATA 
CAGATCACAA CCGACTACGA GATCCTGACC ATCCCTGTGA AAGCTGTGAT CGCCGTGGGC 
TCGCTGACCT GCTTCCCCAA GCACATGGTT CTTCCCCCTT CCTTCCCAGG GAAAATAGTT 
CATCAGAGCT TAAATATTAT GAATTCCTTC TCACAGAAGG TGAAGATACA GCAAATAAGG 
TCTTTGTCAG AAGACGTACG ATTCTACTAC AAACGATTAC GGGGTAATAG GGAAGACTTG 
GAGCCAGGGA AGAAGTCTAA GATTGCAAAC ATTTATTTTG ACCCTGGGCT GCAGTGTGGG 
GATCACTGCT ATATCGGCTT GCCTTTCCTA TCCAAATCTG AACCCAAAGT ACAGCCTGGG 
GTGGCCATGC AGGAGGACTT GTGGGATGCC GACTGGGACG CACATCAGAG CTTGTTCAAG 
GCTTGGATGG GGATAAAGGA AAACGCAGGA CATAGGTTGA ATGCTATGTT TGAAGTAAAT 
ACAGATCTTC AGAAAAATAT CGTATCAAAA GTCAGTGCCG AGCTCTCCTG GCCGTCTGTC 
CTTAGCTCGC CCCGGCTCCT GAAGTTCCCA CTCACTAACA CAAACTGTTC TTCGGAAGAA 
GAGATTAGCT TAGAAAACCC TGCAGACGTC CCTGTCTACG TCCAGTTCAT CCCACTGGCT 
TTGTATTCCA ACCCCTCCGT CTTTGCAGAT AAGTTAGTCT CAAGGTTTAA CTTGAGTAAG 
GTGGCCAAGC TAGATTTGAG AACACTTGAA TTCCAAGTCT ACAGGAACAG CGCTCATCCA 
CTGCAAAGTC CAACAGGATT TACAGAGGGT CTCTCTCGGC ATTTCATTTT AAACCTCATT 
TTAAAACCTG GAGAAAAGAA ATCTGTCAAA GTAAAGTTCA CTCCACTCCA CAACAGAACT 
GTGTCTTCCC TGATCATAGT CAGAAACAAC CTGACTGTGA TGGACGCTGT GATGGTTCAA 
GGTCAAGGGA CAACGGAGAA CTTGAGGGTG GCAGGCAAGC TTCCAGGTCC AGGAAGCTCC 
TTACGCTTCA AAATCACAGA AGCATTGTTA AAGGATTGCA TAGACCGTTT GAAACTAAGA 
GAACCAAATT TCACATTGAA AAGAACATTC AAAGTAGAGA ATACGGGACA ACTTGAAATC 
CGTGTAGAAA CCATTGAGAT CAGTGGATAT GCGTGTGAGG GATATGGTTT TAAAGTGGTT 
AACTGTCAAG AGTTTGCCCT GAGTGCCAAC GCCTCCAGAG ACATAGTCAT ATTGTTTACT 
CCAGATTTCA CAGCCTCCAG AGTCATTCGG GAGCTGAAGT TTGTGACAAG CAGTGGCTCC 
GAGTTTGTGT TTGTGTTGAA TGCCTCTCTT CCGTACCACA TGCTAGCCGC CTGTGCAGAA 
GCCCTCCCTA GACCCAACTG GGAGCTCGCG CTCTACATCA TCATCTCCGG GGTCATGAGT 
GCACTCTTTC TCCTGGTCAT TGGAACAGCC TACTTGGAAG CTCAAGGGAT TTGGGAGCCC 
TTCCGAAGGC GACTCTCCTT TGAAGCCTCA AACCCGCCCT TTGATGTAGG AAGGCCATTT 
GATCTCAGGA GAATCGTTGG TATTTCATCT GAAGGAAACT TGAACACACT TGGCTGCGAA 
CACAGTCATG GTAGGGGTTT CTATAGTAAC GCATCATCCC GGCCTGGTAC AGGCAGTCAC 
AGGCAGTGTG GCACATCAGT CCACCCACAC AGCAGCCATG GCAGCAAAAA CTCAGCTGAT 
GTGGACAACG TCAGAACCAG AAACAGTTCA AGTATGTCAA GCAGGACTTC TCCACAAGCA 
GCTGCCTCAC AGTCTACAAG CAAAACCAGC CCCCTAGTCT CAGAGACAGC TGCAGCAACC 
CAGGGTCACA CAGCAAGCAG GAAGTCCAGA GGGGCAAAGC AGGGCCAGCA CAGCAGCCAG 
CACCACAGCC ACAGCCACAG CCCACTGGAG CAGCACTCAC AGCCTCCACC ACCAGTGCCT 
CAGCACCAGG AGCCACCACC CGAAAGGCTG TCTCCTGCCC CCCTCACACA CCCTTCCCAC 
CCCGAGCGAG CCAGCACCAC ACGGCACAGC TCAGAGGACT CCGACATCAC CAGTCTCATA 
GAAGCCATGG ACAAAGACTT TGACCACCAC GACTCCTCCC CCCTAGACGT GTTTACAGAG 
CAGCCTCCGT CACCGATGTC AAAAAGCAAA GGGAAAGGAA AGTCTCTCCA ACAACGTAAG 
GCCAAGCCTC CGAAGAAGCA GGAGGAGAAG GAGAAGAGGG GGAAGGGGAA GCCGCAGGAG 
GATGAGCTGA AGGACGCTCT GGCCGACGAC GACAGCTCCT CCACCACCAC AGAGACCTCC 
AACCCCGACA CAGAGCCACT CCTCAGGGAG GATACAGAGA AGCACAAGGG GAGACCAGCC 
GTGCCTGAAA AACAGGAAAG TGAACTGTCT CAAGGGAAGC CCAAGAGCAA AAAGCTCTTA 
AACGCTAAGA AAGAAATCCC AACAGATGTG AAAGGCAGTT CCTTTGAACT ACCGTACACT 
CCCTCACTGG AAAATAAACA GCGTAGAAAT CTCCCAACCA AGATTCCTCT TCCAACTACA 
CTAGCAAGTG GATCCAAATC ACGAAATCCC CCGAAAACAA AAGGTACAAA TAAGTTAGTG 
GAAAACAGGC CAGTTGCCCT CTCAAAGTTC CTCCCCAGCA GTCAAGAACT AGGTAATACC 
AGCAGCTCAG AGGGTGAAAA GGATTCTCCT CCACCAGAAT GGGATGCTGT GCCAGTTCAC 
AAGCCCAGCA GCTCTACTGA CAGTCTTTAT AAACTGTCTC TGCAAACCCT CAATGCAGAC 
ATTTTCTTAA AACAACGCCA GACCTCGCCA ACACCTGCCT CTCCTTCTCT GCCCACCGCT 
CCCTGCCCGT TCACATCTCG TGGCAGCTAC AGCAGCGTTG TCAACAGCTC CGGCAGTGAC 
ACAAAAGCCA AGCAGACGAG CAGCAGCAAG AGCAAGCTGA CAAAGGCGGC CTCGCTCCCC 
GGCAAGAATG GCAACCCTAC CTTCGCCGCC GTCGCTGCAG GCTATGACAA GAGCCCGGGT 
GGGAATGGAT TTGCTAAAAT TTCTTCAAAC AAATCAGATT TTTCCAGCAG CCTTGGCATT 
TCACACATTC CTGTTGACAG CGATGGCTCT GACAGCTCGG GTTTGTGGAG TCCTGTCAGC 
AACCCCAACA GCCCTGACTT CACCCCCCTC AATTCGTTCT CAGCCTTTGG AAATTCTTTT 
AATCTAACAG GTGCAGTTTT CAGCAAACTG TCGCGATCCT GCAGTCAGTC GTCCCAGAGA 
AGTTGGAATG AGTTCAACAG CGGCCCCTCC TACCTTTGGG ATTCTCCAGC CACAGACCCC 
AGCCCTTCCT GGCCGGCCAG TTCCAGCTCC CCAACCCACA CAGCCACTTC CATCCTTGGC 
AACAGCAGCG GCTTGTGGTC CACCACTCCA TTCAGCAGCT CCATCTGGTC CAGCAACATC 
AACAGCAACC TTCCTTTCTC CACTCCAACA AACGCGCTGT CGAGCATCAG CCTCATGGGC 
ACGGAAAACT CTGCCGCCGC CCACACCCCT TCCGCCTCTG GCCCAGCTGA CGACTTGGGA 
CAGACCTACA ACCCCTGGCG GATCTGGAGC CCCACAGTCG GAAGAAGAAG CTCTGACCCT 
TGGTCTAATT CGCACTTCCC TCATGAAAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmem131 ( NM_018872.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_061360.2 Mus musculus transmembrane protein 131 (Tmem131), mRNA.

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CloneID	OMu20765
Clone ID Related Accession (Same CDS sequence)	NM_018872.2
Accession Version	NM_018872.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	transmembrane protein 131
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK139317.1, CN533410.1, BU058584.1, CB518500.1, CD546146.1, AB093221.1, AK012138.1 and BE956063.1. On Dec 13, 2006 this sequence version replaced NM_018872.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAAGC GGGCGGGAGG AGCAGCCGCC GCCGCCGCCG CCGCCTCCAC GTCCTCCGCG 
GCCGGGCTGG AGCCCGCGGC TGGCCGAGGC GGGGGTCCTC GGAGCGCAGC CGCGGGCCTC 
CTGGGAGCGC TGCACCTGGT GATGACCCTC GTGGTGGCCG CGGCGCGGGC CGAGAAGGAA 
GCATTCATTC AGTCGGAGAG CATAATAGAA GTCCTGCGCT TTGACGACGG AGGGCTACTA 
CAGACTGAGA CAACACTTGG CCTCGGTTCA TATCAACAGA AAAGCATTTC TCTGTACCGG 
GGTAATTGCA GGCCCATACG ATTCGAACCG CCCATGCTGG ATTTCCATGA GCAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTCTACTTA CATAACCCTA GTTCTGAAGA AACGATTACT 
TTAGTATCAA TATCTGCTAC CACATCACAT TTCCATGCAT CGTTTTTTCA AAATAGGAAA 
ATTCTTCCAG GAGGAAACAC ATCATTTGAT GTAGTTTTTC TTGCAAGAGT AGTAGGAAAT 
GTAGAAAATA CTTTATTTAT TAACACATCT AACCATGGGG TGTTCACTTA CCAGGTATTT 
GGTGTTGGGG TTCCAAATCC GTATCGATTA AGGCCATTTC TTGGGGCCAG AGTCCCCGTA 
AACAGCAGCT TTTCACCCAT AATAAACATA CACAATCCTC ACAGTGAGCC TTTACAGGTC 
GTAGAGATGT ACTCGAGTGG AGGTGACCTT CACCTAGAGC TCCCAACAGG TCAGCAAGGA 
GGCACCAGGA AGCTGTGGGA AATCCCTCCT TATGAAACCA AGGGAGTGAT GAGAGCCAGC 
TTTTCCTCCA GAGAAGCAGA CAATCACACA GCCTTCATCC GGATAAAGAC GAATGCATCA 
GACAGCACCG AGTTCATCAT TCTCCCGGTT GAGGTTGAAG TTACAACAGC TCCTGGAATC 
TATTCCTCAA CTGAAATGTT AGATTTTGGA ACACTTAGAA CACAAGATCT ACCAAAGGTT 
TTAAATCTTC ATTTACTAAA TTCAGGAACA AAGGATGTAC CAATAACAAG TGTTCGACCA 
ACACCACAAA ATGACGCTAT CACGGTACAT TTTAAACCAG TTACGTTAAA AGCTTCTGAA 
AGTAAATATA CCAAGGTTGC CAGCATTAGT TTTGATGCTT CGAGAGCAAA AAAGCCATCT 
CAATTTTCTG GGAAAATAAC TGTTAAAGCA AAGGAAAAGA GTTATTCTAA ACTCGAAATC 
CCCTACCAAG CAGAAGTTCT AGATGGTTAT TTGGGGTTTG ATCACGCCGC CACGCTGTTT 
CACATCCAGG ACAGTCCTGC TGACCCAGTG GAGAGGCCGA TCTACCTGAC GAACACTTTC 
AGTTTTGCGA TCCTCATTCA TGATGTGCTG TTACCAGAAG AAGCCAGAAT AATGTTTCAG 
GTGCACAACT TCAGCCAGCC GGTTCTCATT CTTCCTAATG AGTCGGGATA CATTTTTACC 
CTGTTTTTTA TGCCATCCAC CTCATCCATG CACATAGACA ACAACATTTT ACTTGTTACC 
AATGCTTCTA AGTTTCATTT ACCTGTGCGG GTATACACAG GCTTTTTAGA TTACTTTGTA 
TTGCCCCCCA AAATAGAAGA ACGCTTCATA GATTTTGGAG TACTGAGTGC TACAGAAGCA 
AGTAGTATTT TATTTGCAAT TATAAACAGC AATCCAATTG AGCTGGCAAT AAAAAGTTGG 
CATATCATAG GAGATGGCTT ATCAATAGAA CTTGTAGCTA CAGAGAGAGG CAACAGAAGC 
ACGGTGATTG CAAGCCTTCC AGAACTGGAG CGGTCCTCTT TGCCCGACCA GTCCCCGGTA 
ACATTAGCTT CAGGACACTT TGCAGTGTTC AGAGTCAAAC TTACTGCCAA GAAGCTAGAA 
GGGGTCCATG ATGGGGCCAT ACAGATCACA ACCGACTACG AGATCCTGAC CATCCCTGTG 
AAAGCTGTGA TCGCCGTGGG CTCGCTGACC TGCTTCCCCA AGCACATGGT TCTTCCCCCT 
TCCTTCCCAG GGAAAATAGT TCATCAGAGC TTAAATATTA TGAATTCCTT CTCACAGAAG 
GTGAAGATAC AGCAAATAAG GTCTTTGTCA GAAGACGTAC GATTCTACTA CAAACGATTA 
CGGGGTAATA GGGAAGACTT GGAGCCAGGG AAGAAGTCTA AGATTGCAAA CATTTATTTT 
GACCCTGGGC TGCAGTGTGG GGATCACTGC TATATCGGCT TGCCTTTCCT ATCCAAATCT 
GAACCCAAAG TACAGCCTGG GGTGGCCATG CAGGAGGACT TGTGGGATGC CGACTGGGAC 
GCACATCAGA GCTTGTTCAA GGCTTGGATG GGGATAAAGG AAAACGCAGG ACATAGGTTG 
AATGCTATGT TTGAAGTAAA TACAGATCTT CAGAAAAATA TCGTATCAAA AGTCAGTGCC 
GAGCTCTCCT GGCCGTCTGT CCTTAGCTCG CCCCGGCTCC TGAAGTTCCC ACTCACTAAC 
ACAAACTGTT CTTCGGAAGA AGAGATTAGC TTAGAAAACC CTGCAGACGT CCCTGTCTAC 
GTCCAGTTCA TCCCACTGGC TTTGTATTCC AACCCCTCCG TCTTTGCAGA TAAGTTAGTC 
TCAAGGTTTA ACTTGAGTAA GGTGGCCAAG CTAGATTTGA GAACACTTGA ATTCCAAGTC 
TACAGGAACA GCGCTCATCC ACTGCAAAGT CCAACAGGAT TTACAGAGGG TCTCTCTCGG 
CATTTCATTT TAAACCTCAT TTTAAAACCT GGAGAAAAGA AATCTGTCAA AGTAAAGTTC 
ACTCCACTCC ACAACAGAAC TGTGTCTTCC CTGATCATAG TCAGAAACAA CCTGACTGTG 
ATGGACGCTG TGATGGTTCA AGGTCAAGGG ACAACGGAGA ACTTGAGGGT GGCAGGCAAG 
CTTCCAGGTC CAGGAAGCTC CTTACGCTTC AAAATCACAG AAGCATTGTT AAAGGATTGC 
ATAGACCGTT TGAAACTAAG AGAACCAAAT TTCACATTGA AAAGAACATT CAAAGTAGAG 
AATACGGGAC AACTTGAAAT CCGTGTAGAA ACCATTGAGA TCAGTGGATA TGCGTGTGAG 
GGATATGGTT TTAAAGTGGT TAACTGTCAA GAGTTTGCCC TGAGTGCCAA CGCCTCCAGA 
GACATAGTCA TATTGTTTAC TCCAGATTTC ACAGCCTCCA GAGTCATTCG GGAGCTGAAG 
TTTGTGACAA GCAGTGGCTC CGAGTTTGTG TTTGTGTTGA ATGCCTCTCT TCCGTACCAC 
ATGCTAGCCG CCTGTGCAGA AGCCCTCCCT AGACCCAACT GGGAGCTCGC GCTCTACATC 
ATCATCTCCG GGGTCATGAG TGCACTCTTT CTCCTGGTCA TTGGAACAGC CTACTTGGAA 
GCTCAAGGGA TTTGGGAGCC CTTCCGAAGG CGACTCTCCT TTGAAGCCTC AAACCCGCCC 
TTTGATGTAG GAAGGCCATT TGATCTCAGG AGAATCGTTG GTATTTCATC TGAAGGAAAC 
TTGAACACAC TTGGCTGCGA ACACAGTCAT GGTAGGGGTT TCTATAGTAA CGCATCATCC 
CGGCCTGGTA CAGGCAGTCA CAGGCAGTGT GGCACATCAG TCCACCCACA CAGCAGCCAT 
GGCAGCAAAA ACTCAGCTGA TGTGGACAAC GTCAGAACCA GAAACAGTTC AAGTATGTCA 
AGCAGGACTT CTCCACAAGC AGCTGCCTCA CAGTCTACAA GCAAAACCAG CCCCCTAGTC 
TCAGAGACAG CTGCAGCAAC CCAGGGTCAC ACAGCAAGCA GGAAGTCCAG AGGGGCAAAG 
CAGGGCCAGC ACAGCAGCCA GCACCACAGC CACAGCCACA GCCCACTGGA GCAGCACTCA 
CAGCCTCCAC CACCAGTGCC TCAGCACCAG GAGCCACCAC CCGAAAGGCT GTCTCCTGCC 
CCCCTCACAC ACCCTTCCCA CCCCGAGCGA GCCAGCACCA CACGGCACAG CTCAGAGGAC 
TCCGACATCA CCAGTCTCAT AGAAGCCATG GACAAAGACT TTGACCACCA CGACTCCTCC 
CCCCTAGACG TGTTTACAGA GCAGCCTCCG TCACCGATGT CAAAAAGCAA AGGGAAAGGA 
AAGTCTCTCC AACAACGTAA GGCCAAGCCT CCGAAGAAGC AGGAGGAGAA GGAGAAGAGG 
GGGAAGGGGA AGCCGCAGGA GGATGAGCTG AAGGACGCTC TGGCCGACGA CGACAGCTCC 
TCCACCACCA CAGAGACCTC CAACCCCGAC ACAGAGCCAC TCCTCAGGGA GGATACAGAG 
AAGCACAAGG GGAGACCAGC CGTGCCTGAA AAACAGGAAA GTGAACTGTC TCAAGGGAAG 
CCCAAGAGCA AAAAGCTCTT AAACGCTAAG AAAGAAATCC CAACAGATGT GAAAGGCAGT 
TCCTTTGAAC TACCGTACAC TCCCTCACTG GAAAATAAAC AGCGTAGAAA TCTCCCAACC 
AAGATTCCTC TTCCAACTAC ACTAGCAAGT GGATCCAAAT CACGAAATCC CCCGAAAACA 
AAAGGTACAA ATAAGTTAGT GGAAAACAGG CCAGTTGCCC TCTCAAAGTT CCTCCCCAGC 
AGTCAAGAAC TAGGTAATAC CAGCAGCTCA GAGGGTGAAA AGGATTCTCC TCCACCAGAA 
TGGGATGCTG TGCCAGTTCA CAAGCCCAGC AGCTCTACTG ACAGTCTTTA TAAACTGTCT 
CTGCAAACCC TCAATGCAGA CATTTTCTTA AAACAACGCC AGACCTCGCC AACACCTGCC 
TCTCCTTCTC TGCCCACCGC TCCCTGCCCG TTCACATCTC GTGGCAGCTA CAGCAGCGTT 
GTCAACAGCT CCGGCAGTGA CACAAAAGCC AAGCAGACGA GCAGCAGCAA GAGCAAGCTG 
ACAAAGGCGG CCTCGCTCCC CGGCAAGAAT GGCAACCCTA CCTTCGCCGC CGTCGCTGCA 
GGCTATGACA AGAGCCCGGG TGGGAATGGA TTTGCTAAAA TTTCTTCAAA CAAATCAGAT 
TTTTCCAGCA GCCTTGGCAT TTCACACATT CCTGTTGACA GCGATGGCTC TGACAGCTCG 
GGTTTGTGGA GTCCTGTCAG CAACCCCAAC AGCCCTGACT TCACCCCCCT CAATTCGTTC 
TCAGCCTTTG GAAATTCTTT TAATCTAACA GGTGCAGTTT TCAGCAAACT GTCGCGATCC 
TGCAGTCAGT CGTCCCAGAG AAGTTGGAAT GAGTTCAACA GCGGCCCCTC CTACCTTTGG 
GATTCTCCAG CCACAGACCC CAGCCCTTCC TGGCCGGCCA GTTCCAGCTC CCCAACCCAC 
ACAGCCACTT CCATCCTTGG CAACAGCAGC GGCTTGTGGT CCACCACTCC ATTCAGCAGC 
TCCATCTGGT CCAGCAACAT CAACAGCAAC CTTCCTTTCT CCACTCCAAC AAACGCGCTG 
TCGAGCATCA GCCTCATGGG CACGGAAAAC TCTGCCGCCG CCCACACCCC TTCCGCCTCT 
GGCCCAGCTG ACGACTTGGG ACAGACCTAC AACCCCTGGC GGATCTGGAG CCCCACAGTC 
GGAAGAAGAA GCTCTGACCC TTGGTCTAAT TCGCACTTCC CTCATGAAAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061360.2
CDS	183..5816
Translation	MGKRAGGAAAAAAAASTSSAAGLEPAAGRGGGPRSAAAGLLGALHLVMTLVVAAARAEKEAFIQSESIIEVLRFDDGGLLQTETTLGLGSYQQKSISLYRGNCRPIRFEPPMLDFHEQPVGMPKMEKVYLHNPSSEETITLVSISATTSHFHASFFQNRKILPGGNTSFDVVFLARVVGNVENTLFINTSNHGVFTYQVFGVGVPNPYRLRPFLGARVPVNSSFSPIINIHNPHSEPLQVVEMYSSGGDLHLELPTGQQGGTRKLWEIPPYETKGVMRASFSSREADNHTAFIRIKTNASDSTEFIILPVEVEVTTAPGIYSSTEMLDFGTLRTQDLPKVLNLHLLNSGTKDVPITSVRPTPQNDAITVHFKPVTLKASESKYTKVASISFDASRAKKPSQFSGKITVKAKEKSYSKLEIPYQAEVLDGYLGFDHAATLFHIQDSPADPVERPIYLTNTFSFAILIHDVLLPEEARIMFQVHNFSQPVLILPNESGYIFTLFFMPSTSSMHIDNNILLVTNASKFHLPVRVYTGFLDYFVLPPKIEERFIDFGVLSATEASSILFAIINSNPIELAIKSWHIIGDGLSIELVATERGNRSTVIASLPELERSSLPDQSPVTLASGHFAVFRVKLTAKKLEGVHDGAIQITTDYEILTIPVKAVIAVGSLTCFPKHMVLPPSFPGKIVHQSLNIMNSFSQKVKIQQIRSLSEDVRFYYKRLRGNREDLEPGKKSKIANIYFDPGLQCGDHCYIGLPFLSKSEPKVQPGVAMQEDLWDADWDAHQSLFKAWMGIKENAGHRLNAMFEVNTDLQKNIVSKVSAELSWPSVLSSPRLLKFPLTNTNCSSEEEISLENPADVPVYVQFIPLALYSNPSVFADKLVSRFNLSKVAKLDLRTLEFQVYRNSAHPLQSPTGFTEGLSRHFILNLILKPGEKKSVKVKFTPLHNRTVSSLIIVRNNLTVMDAVMVQGQGTTENLRVAGKLPGPGSSLRFKITEALLKDCIDRLKLREPNFTLKRTFKVENTGQLEIRVETIEISGYACEGYGFKVVNCQEFALSANASRDIVILFTPDFTASRVIRELKFVTSSGSEFVFVLNASLPYHMLAACAEALPRPNWELALYIIISGVMSALFLLVIGTAYLEAQGIWEPFRRRLSFEASNPPFDVGRPFDLRRIVGISSEGNLNTLGCEHSHGRGFYSNASSRPGTGSHRQCGTSVHPHSSHGSKNSADVDNVRTRNSSSMSSRTSPQAAASQSTSKTSPLVSETAAATQGHTASRKSRGAKQGQHSSQHHSHSHSPLEQHSQPPPPVPQHQEPPPERLSPAPLTHPSHPERASTTRHSSEDSDITSLIEAMDKDFDHHDSSPLDVFTEQPPSPMSKSKGKGKSLQQRKAKPPKKQEEKEKRGKGKPQEDELKDALADDDSSSTTTETSNPDTEPLLREDTEKHKGRPAVPEKQESELSQGKPKSKKLLNAKKEIPTDVKGSSFELPYTPSLENKQRRNLPTKIPLPTTLASGSKSRNPPKTKGTNKLVENRPVALSKFLPSSQELGNTSSSEGEKDSPPPEWDAVPVHKPSSSTDSLYKLSLQTLNADIFLKQRQTSPTPASPSLPTAPCPFTSRGSYSSVVNSSGSDTKAKQTSSSKSKLTKAASLPGKNGNPTFAAVAAGYDKSPGGNGFAKISSNKSDFSSSLGISHIPVDSDGSDSSGLWSPVSNPNSPDFTPLNSFSAFGNSFNLTGAVFSKLSRSCSQSSQRSWNEFNSGPSYLWDSPATDPSPSWPASSSSPTHTATSILGNSSGLWSTTPFSSSIWSSNINSNLPFSTPTNALSSISLMGTENSAAAHTPSASGPADDLGQTYNPWRIWSPTVGRRSSDPWSNSHFPHEN

Target ORF information:

RefSeq Version	NM_018872.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane protein 131 (Tmem131), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018872.2

ORF Insert Sequence:

ATGGGGAAGC GGGCGGGAGG AGCAGCCGCC GCCGCCGCCG CCGCCTCCAC GTCCTCCGCG 
GCCGGGCTGG AGCCCGCGGC TGGCCGAGGC GGGGGTCCTC GGAGCGCAGC CGCGGGCCTC 
CTGGGAGCGC TGCACCTGGT GATGACCCTC GTGGTGGCCG CGGCGCGGGC CGAGAAGGAA 
GCATTCATTC AGTCGGAGAG CATAATAGAA GTCCTGCGCT TTGACGACGG AGGGCTACTA 
CAGACTGAGA CAACACTTGG CCTCGGTTCA TATCAACAGA AAAGCATTTC TCTGTACCGG 
GGTAATTGCA GGCCCATACG ATTCGAACCG CCCATGCTGG ATTTCCATGA GCAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTCTACTTA CATAACCCTA GTTCTGAAGA AACGATTACT 
TTAGTATCAA TATCTGCTAC CACATCACAT TTCCATGCAT CGTTTTTTCA AAATAGGAAA 
ATTCTTCCAG GAGGAAACAC ATCATTTGAT GTAGTTTTTC TTGCAAGAGT AGTAGGAAAT 
GTAGAAAATA CTTTATTTAT TAACACATCT AACCATGGGG TGTTCACTTA CCAGGTATTT 
GGTGTTGGGG TTCCAAATCC GTATCGATTA AGGCCATTTC TTGGGGCCAG AGTCCCCGTA 
AACAGCAGCT TTTCACCCAT AATAAACATA CACAATCCTC ACAGTGAGCC TTTACAGGTC 
GTAGAGATGT ACTCGAGTGG AGGTGACCTT CACCTAGAGC TCCCAACAGG TCAGCAAGGA 
GGCACCAGGA AGCTGTGGGA AATCCCTCCT TATGAAACCA AGGGAGTGAT GAGAGCCAGC 
TTTTCCTCCA GAGAAGCAGA CAATCACACA GCCTTCATCC GGATAAAGAC GAATGCATCA 
GACAGCACCG AGTTCATCAT TCTCCCGGTT GAGGTTGAAG TTACAACAGC TCCTGGAATC 
TATTCCTCAA CTGAAATGTT AGATTTTGGA ACACTTAGAA CACAAGATCT ACCAAAGGTT 
TTAAATCTTC ATTTACTAAA TTCAGGAACA AAGGATGTAC CAATAACAAG TGTTCGACCA 
ACACCACAAA ATGACGCTAT CACGGTACAT TTTAAACCAG TTACGTTAAA AGCTTCTGAA 
AGTAAATATA CCAAGGTTGC CAGCATTAGT TTTGATGCTT CGAGAGCAAA AAAGCCATCT 
CAATTTTCTG GGAAAATAAC TGTTAAAGCA AAGGAAAAGA GTTATTCTAA ACTCGAAATC 
CCCTACCAAG CAGAAGTTCT AGATGGTTAT TTGGGGTTTG ATCACGCCGC CACGCTGTTT 
CACATCCAGG ACAGTCCTGC TGACCCAGTG GAGAGGCCGA TCTACCTGAC GAACACTTTC 
AGTTTTGCGA TCCTCATTCA TGATGTGCTG TTACCAGAAG AAGCCAGAAT AATGTTTCAG 
GTGCACAACT TCAGCCAGCC GGTTCTCATT CTTCCTAATG AGTCGGGATA CATTTTTACC 
CTGTTTTTTA TGCCATCCAC CTCATCCATG CACATAGACA ACAACATTTT ACTTGTTACC 
AATGCTTCTA AGTTTCATTT ACCTGTGCGG GTATACACAG GCTTTTTAGA TTACTTTGTA 
TTGCCCCCCA AAATAGAAGA ACGCTTCATA GATTTTGGAG TACTGAGTGC TACAGAAGCA 
AGTAGTATTT TATTTGCAAT TATAAACAGC AATCCAATTG AGCTGGCAAT AAAAAGTTGG 
CATATCATAG GAGATGGCTT ATCAATAGAA CTTGTAGCTA CAGAGAGAGG CAACAGAAGC 
ACGGTGATTG CAAGCCTTCC AGAACTGGAG CGGTCCTCTT TGCCCGACCA GTCCCCGGTA 
ACATTAGCTT CAGGACACTT TGCAGTGTTC AGAGTCAAAC TTACTGCCAA GAAGCTAGAA 
GGGGTCCATG ATGGGGCCAT ACAGATCACA ACCGACTACG AGATCCTGAC CATCCCTGTG 
AAAGCTGTGA TCGCCGTGGG CTCGCTGACC TGCTTCCCCA AGCACATGGT TCTTCCCCCT 
TCCTTCCCAG GGAAAATAGT TCATCAGAGC TTAAATATTA TGAATTCCTT CTCACAGAAG 
GTGAAGATAC AGCAAATAAG GTCTTTGTCA GAAGACGTAC GATTCTACTA CAAACGATTA 
CGGGGTAATA GGGAAGACTT GGAGCCAGGG AAGAAGTCTA AGATTGCAAA CATTTATTTT 
GACCCTGGGC TGCAGTGTGG GGATCACTGC TATATCGGCT TGCCTTTCCT ATCCAAATCT 
GAACCCAAAG TACAGCCTGG GGTGGCCATG CAGGAGGACT TGTGGGATGC CGACTGGGAC 
GCACATCAGA GCTTGTTCAA GGCTTGGATG GGGATAAAGG AAAACGCAGG ACATAGGTTG 
AATGCTATGT TTGAAGTAAA TACAGATCTT CAGAAAAATA TCGTATCAAA AGTCAGTGCC 
GAGCTCTCCT GGCCGTCTGT CCTTAGCTCG CCCCGGCTCC TGAAGTTCCC ACTCACTAAC 
ACAAACTGTT CTTCGGAAGA AGAGATTAGC TTAGAAAACC CTGCAGACGT CCCTGTCTAC 
GTCCAGTTCA TCCCACTGGC TTTGTATTCC AACCCCTCCG TCTTTGCAGA TAAGTTAGTC 
TCAAGGTTTA ACTTGAGTAA GGTGGCCAAG CTAGATTTGA GAACACTTGA ATTCCAAGTC 
TACAGGAACA GCGCTCATCC ACTGCAAAGT CCAACAGGAT TTACAGAGGG TCTCTCTCGG 
CATTTCATTT TAAACCTCAT TTTAAAACCT GGAGAAAAGA AATCTGTCAA AGTAAAGTTC 
ACTCCACTCC ACAACAGAAC TGTGTCTTCC CTGATCATAG TCAGAAACAA CCTGACTGTG 
ATGGACGCTG TGATGGTTCA AGGTCAAGGG ACAACGGAGA ACTTGAGGGT GGCAGGCAAG 
CTTCCAGGTC CAGGAAGCTC CTTACGCTTC AAAATCACAG AAGCATTGTT AAAGGATTGC 
ATAGACCGTT TGAAACTAAG AGAACCAAAT TTCACATTGA AAAGAACATT CAAAGTAGAG 
AATACGGGAC AACTTGAAAT CCGTGTAGAA ACCATTGAGA TCAGTGGATA TGCGTGTGAG 
GGATATGGTT TTAAAGTGGT TAACTGTCAA GAGTTTGCCC TGAGTGCCAA CGCCTCCAGA 
GACATAGTCA TATTGTTTAC TCCAGATTTC ACAGCCTCCA GAGTCATTCG GGAGCTGAAG 
TTTGTGACAA GCAGTGGCTC CGAGTTTGTG TTTGTGTTGA ATGCCTCTCT TCCGTACCAC 
ATGCTAGCCG CCTGTGCAGA AGCCCTCCCT AGACCCAACT GGGAGCTCGC GCTCTACATC 
ATCATCTCCG GGGTCATGAG TGCACTCTTT CTCCTGGTCA TTGGAACAGC CTACTTGGAA 
GCTCAAGGGA TTTGGGAGCC CTTCCGAAGG CGACTCTCCT TTGAAGCCTC AAACCCGCCC 
TTTGATGTAG GAAGGCCATT TGATCTCAGG AGAATCGTTG GTATTTCATC TGAAGGAAAC 
TTGAACACAC TTGGCTGCGA ACACAGTCAT GGTAGGGGTT TCTATAGTAA CGCATCATCC 
CGGCCTGGTA CAGGCAGTCA CAGGCAGTGT GGCACATCAG TCCACCCACA CAGCAGCCAT 
GGCAGCAAAA ACTCAGCTGA TGTGGACAAC GTCAGAACCA GAAACAGTTC AAGTATGTCA 
AGCAGGACTT CTCCACAAGC AGCTGCCTCA CAGTCTACAA GCAAAACCAG CCCCCTAGTC 
TCAGAGACAG CTGCAGCAAC CCAGGGTCAC ACAGCAAGCA GGAAGTCCAG AGGGGCAAAG 
CAGGGCCAGC ACAGCAGCCA GCACCACAGC CACAGCCACA GCCCACTGGA GCAGCACTCA 
CAGCCTCCAC CACCAGTGCC TCAGCACCAG GAGCCACCAC CCGAAAGGCT GTCTCCTGCC 
CCCCTCACAC ACCCTTCCCA CCCCGAGCGA GCCAGCACCA CACGGCACAG CTCAGAGGAC 
TCCGACATCA CCAGTCTCAT AGAAGCCATG GACAAAGACT TTGACCACCA CGACTCCTCC 
CCCCTAGACG TGTTTACAGA GCAGCCTCCG TCACCGATGT CAAAAAGCAA AGGGAAAGGA 
AAGTCTCTCC AACAACGTAA GGCCAAGCCT CCGAAGAAGC AGGAGGAGAA GGAGAAGAGG 
GGGAAGGGGA AGCCGCAGGA GGATGAGCTG AAGGACGCTC TGGCCGACGA CGACAGCTCC 
TCCACCACCA CAGAGACCTC CAACCCCGAC ACAGAGCCAC TCCTCAGGGA GGATACAGAG 
AAGCACAAGG GGAGACCAGC CGTGCCTGAA AAACAGGAAA GTGAACTGTC TCAAGGGAAG 
CCCAAGAGCA AAAAGCTCTT AAACGCTAAG AAAGAAATCC CAACAGATGT GAAAGGCAGT 
TCCTTTGAAC TACCGTACAC TCCCTCACTG GAAAATAAAC AGCGTAGAAA TCTCCCAACC 
AAGATTCCTC TTCCAACTAC ACTAGCAAGT GGATCCAAAT CACGAAATCC CCCGAAAACA 
AAAGGTACAA ATAAGTTAGT GGAAAACAGG CCAGTTGCCC TCTCAAAGTT CCTCCCCAGC 
AGTCAAGAAC TAGGTAATAC CAGCAGCTCA GAGGGTGAAA AGGATTCTCC TCCACCAGAA 
TGGGATGCTG TGCCAGTTCA CAAGCCCAGC AGCTCTACTG ACAGTCTTTA TAAACTGTCT 
CTGCAAACCC TCAATGCAGA CATTTTCTTA AAACAACGCC AGACCTCGCC AACACCTGCC 
TCTCCTTCTC TGCCCACCGC TCCCTGCCCG TTCACATCTC GTGGCAGCTA CAGCAGCGTT 
GTCAACAGCT CCGGCAGTGA CACAAAAGCC AAGCAGACGA GCAGCAGCAA GAGCAAGCTG 
ACAAAGGCGG CCTCGCTCCC CGGCAAGAAT GGCAACCCTA CCTTCGCCGC CGTCGCTGCA 
GGCTATGACA AGAGCCCGGG TGGGAATGGA TTTGCTAAAA TTTCTTCAAA CAAATCAGAT 
TTTTCCAGCA GCCTTGGCAT TTCACACATT CCTGTTGACA GCGATGGCTC TGACAGCTCG 
GGTTTGTGGA GTCCTGTCAG CAACCCCAAC AGCCCTGACT TCACCCCCCT CAATTCGTTC 
TCAGCCTTTG GAAATTCTTT TAATCTAACA GGTGCAGTTT TCAGCAAACT GTCGCGATCC 
TGCAGTCAGT CGTCCCAGAG AAGTTGGAAT GAGTTCAACA GCGGCCCCTC CTACCTTTGG 
GATTCTCCAG CCACAGACCC CAGCCCTTCC TGGCCGGCCA GTTCCAGCTC CCCAACCCAC 
ACAGCCACTT CCATCCTTGG CAACAGCAGC GGCTTGTGGT CCACCACTCC ATTCAGCAGC 
TCCATCTGGT CCAGCAACAT CAACAGCAAC CTTCCTTTCT CCACTCCAAC AAACGCGCTG 
TCGAGCATCA GCCTCATGGG CACGGAAAAC TCTGCCGCCG CCCACACCCC TTCCGCCTCT 
GGCCCAGCTG ACGACTTGGG ACAGACCTAC AACCCCTGGC GGATCTGGAG CCCCACAGTC 
GGAAGAAGAA GCTCTGACCC TTGGTCTAAT TCGCACTTCC CTCATGAAAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmem131 ( XM_006496150.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496213.1 Mus musculus transmembrane protein 131 (Tmem131), transcript variant X1, mRNA.

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CloneID	OMu27534
Clone ID Related Accession (Same CDS sequence)	XM_006496150.2 , XM_006496150.3
Accession Version	XM_006496150.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	transmembrane protein 131 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496150.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGC GGGCGGGAGG AGCAGCCGCC GCCGCCGCCG CCGCCTCCAC GTCCTCCGCG 
GCCGGGCTGG AGCCCGCGGC TGGCCGAGGC GGGGGTCCTC GGAGCGCAGC CGCGGGCCTC 
CTGGGAGCGC TGCACCTGGT GATGACCCTC GTGGTGGCCG CGGCGCGGGC CGAGAAGGAA 
GCATTCATTC AGTCGGAGAG CATAATAGAA GTCCTGCGCT TTGACGACGG AGGGCTACTA 
CAGACTGAGA CAACACTTGG CCTCGGTTCA TATCAACAGA AAAGCATTTC TCTGTACCGG 
GGTAATTGCA GGCCCATACG ATTCGAACCG CCCATGCTGG ATTTCCATGA GCAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTCTACTTA CATAACCCTA GTTCTGAAGA AACGATTACT 
TTAGTATCAA TATCTGCTAC CACATCACAT TTCCATGCAT CGTTTTTTCA AAATAGGAAA 
ATTCTTCCAG GAGGAAACAC ATCATTTGAT GTAGTTTTTC TTGCAAGAGT AGTAGGAAAT 
GTAGAAAATA CTTTATTTAT TAACACATCT AACCATGGGG TGTTCACTTA CCAGGTATTT 
GGTGTTGGGG TTCCAAATCC GTATCGATTA AGGCCATTTC TTGGGGCCAG AGTCCCCGTA 
AACAGCAGCT TTTCACCCAT AATAAACATA CACAATCCTC ACAGTGAGCC TTTACAGGTC 
GTAGAGATGT ACTCGAGTGG AGGTGACCTT CACCTAGAGC TCCCAACAGG TCAGCAAGGA 
GGCACCAGGA AGCTGTGGGA AATCCCTCCT TATGAAACCA AGGGAGTGAT GAGAGCCAGC 
TTTTCCTCCA GAGAAGCAGA CAATCACACA GCCTTCATCC GGATAAAGAC GAATGCATCA 
GACAGCACCG AGTTCATCAT TCTCCCGGTT GAGGTTGAAG TTACAACAGC TCCTGGAATC 
TATTCCTCAA CTGAAATGTT AGATTTTGGA ACACTTAGAA CACAAGATCT ACCAAAGGTT 
TTAAATCTTC ATTTACTAAA TTCAGGAACA AAGGATGTAC CAATAACAAG TGTTCGACCA 
ACACCACAAA ATGACGCTAT CACGGTACAT TTTAAACCAG TTACGTTAAA AGCTTCTGAA 
AGTAAATATA CCAAGGTTGC CAGCATTAGT TTTGATGCTT CGAGAGCAAA AAAGCCATCT 
CAATTTTCTG GGAAAATAAC TGTTAAAGCA AAGGAAAAGA GTTATTCTAA ACTCGAAATC 
CCCTACCAAG CAGAAGTTCT AGATGGTTAT TTGGGGTTTG ATCACGCCGC CACGCTGTTT 
CACATCCAGG ACAGTCCTGC TGACCCAGTG GAGAGGCCGA TCTACCTGAC GAACACTTTC 
AGTTTTGCGA TCCTCATTCA TGATGTGCTG TTACCAGAAG AAGCCAGAAT AATGTTTCAG 
GTGCACAACT TCAGCCAGCC GGTTCTCATT CTTCCTAATG AGTCGGGATA CATTTTTACC 
CTGTTTTTTA TGCCATCCAC CTCATCCATG CACATAGACA ACAACATTTT ACTTGTTACC 
AATGCTTCTA AGTTTCATTT ACCTGTGCGG GTATACACAG GCTTTTTAGA TTACTTTGTA 
TTGCCCCCCA AAATAGAAGA ACGCTTCATA GATTTTGGAG TACTGAGTGC TACAGAAGCA 
AGTAGTATTT TATTTGCAAT TATAAACAGC AATCCAATTG AGCTGGCAAT AAAAAGTTGG 
CATATCATAG GAGATGGCTT ATCAATAGAA CTTGTAGCTA CAGAGAGAGG CAACAGAAGC 
ACGGTGATTG CAAGCCTTCC AGAACTGGAG CGGTCCTCTT TGCCCGACCA GTCCCCGGTA 
ACATTAGCTT CAGGACACTT TGCAGTGTTC AGAGTCAAAC TTACTGCCAA GAAGCTAGAA 
GGGGTCCATG ATGGGGCCAT ACAGATCACA ACCGACTACG AGATCCTGAC CATCCCTGTG 
AAAGCTGTGA TCGCCGTGGG CTCGCTGACC TGCTTCCCCA AGCACATGGT TCTTCCCCCT 
TCCTTCCCAG GGAAAATAGT TCATCAGAGC TTAAATATTA TGAATTCCTT CTCACAGAAG 
GTGAAGATAC AGCAAATAAG GTCTTTGTCA GAAGACGTAC GATTCTACTA CAAACGATTA 
CGGGGTAATA GGGAAGACTT GGAGCCAGGG AAGAAGTCTA AGATTGCAAA CATTTATTTT 
GACCCTGGGC TGCAGTGTGG GGATCACTGC TATATCGGCT TGCCTTTCCT ATCCAAATCT 
GAACCCAAAG TACAGCCTGG GGTGGCCATG CAGGAGGACT TGTGGGATGC CGACTGGGAC 
GCACATCAGA GCTTGTTCAA GGCTTGGATG GGGATAAAGG AAAACGCAGG ACATAGGTTG 
AATGCTATGT TTGAAGTAAA TACAGATCTT CAGAAAAATA TCGTATCAAA AGTCAGTGCC 
GAGCTCTCCT GGCCGTCTGT CCTTAGCTCG CCCCGGCTCC TGAAGTTCCC ACTCACTAAC 
ACAAACTGTT CTTCGGAAGA AGAGATTAGC TTAGAAAACC CTGCAGACGT CCCTGTCTAC 
GTCCAGTTCA TCCCACTGGC TTTGTATTCC AACCCCTCCG TCTTTGCAGA TAAGTTAGTC 
TCAAGGTTTA ACTTGAGTAA GGTGGCCAAG CTAGATTTGA GAACACTTGA ATTCCAAGTC 
TACAGGAACA GCGCTCATCC ACTGCAAAGT CCAACAGGAT TTACAGAGGG TCTCTCTCGG 
CATTTCATTT TAAACCTCAT TTTAAAACCT GGAGAAAAGA AATCTGTCAA AGTAAAGTTC 
ACTCCACTCC ACAACAGAAC TGTGTCTTCC CTGATCATAG TCAGAAACAA CCTGACTGTG 
ATGGACGCTG TGATGGTTCA AGGTCAAGGG ACAACGGAGA ACTTGAGGGT GGCAGGCAAG 
CTTCCAGGTC CAGGAAGCTC CTTACGCTTC AAAATCACAG AAGCATTGTT AAAGGATTGC 
ATAGACCGTT TGAAACTAAG AGAACCAAAT TTCACATTGA AAAGAACATT CAAAGTAGAG 
AATACGGGAC AACTTGAAAT CCGTGTAGAA ACCATTGAGA TCAGTGGATA TGCGTGTGAG 
GGATATGGTT TTAAAGTGGT TAACTGTCAA GAGTTTGCCC TGAGTGCCAA CGCCTCCAGA 
GACATAGTCA TATTGTTTAC TCCAGATTTC ACAGCCTCCA GAGTCATTCG GGAGCTGAAG 
TTTGTGACAA GCAGTGGCTC CGAGTTTGTG TTTGTGTTGA ATGCCTCTCT TCCGTACCAC 
ATGCTAGCCG CCTGTGCAGA AGCCCTCCCT AGACCCAACT GGGAGCTCGC GCTCTACATC 
ATCATCTCCG GGGTCATGAG TGCACTCTTT CTCCTGGTCA TTGGAACAGC CTACTTGGAA 
GCTCAAGGGA TTTGGGAGCC CTTCCGAAGG CGACTCTCCT TTGAAGCCTC AAACCCGCCC 
TTTGATGTAG GAAGGCCATT TGATCTCAGG AGAATCGTTG GTATTTCATC TGAAGGAAAC 
TTGAACACAC TTGGCTGCGA ACACAGTCAT GGTAGGGGTT TCTATAGTAA CGCATCATCC 
CGGCCTGGTA CAGGCAGTCA CAGGCAGTGT GGCACATCAG TCCACCCACA CAGCAGCCAT 
GGCAGCAAAA ACTCAGCTGA TGTGGACAAC GTCAGAACCA GAAACAGTTC AAGTATGTCA 
AGCAGGACTT CTCCACAAGC AGCTGCCTCA CAGTCTACAA GCAAAACCAG CCCCCTAGTC 
TCAGAGACAG CTGCAGCAAC CCAGGGTCAC ACAGCAAGCA GGAAGTCCAG AGGGGCAAAG 
CAGGGCCAGC ACAGCAGCCA GCACCACAGC CACAGCCACA GCCCACTGGA GCAGCACTCA 
CAGCCTCCAC CACCAGTGCC TCAGCACCAG GAGCCACCAC CCGAAAGGCT GTCTCCTGCC 
CCCCTCACAC ACCCTTCCCA CCCCGAGCGA GCCAGCACCA CACGGCACAG CTCAGAGGAC 
TCCGACATCA CCAGTCTCAT AGAAGCCATG GACAAAGACT TTGACCACCA CGACTCCTCC 
CCCCTAGACG TGTTTACAGA GCAGCCTCCG TCACCGATGT CAAAAAGCAA AGGGAAAGGA 
AAGTCTCTCC AACAACGTAA GGCCAAGCCT CCGAAGAAGC AGGAGGAGAA GGAGAAGAGG 
GGGAAGGGGA AGCCGCAGGA GGATGAGCTG AAGGACGCTC TGGCCGACGA CGACAGCTCC 
TCCACCACCA CAGAGACCTC CAACCCCGAC ACAGAGCCAC TCCTCAGGGA GGATACAGAG 
AAGCACAAGG GGAGACCAGC CGTGCCTGAA AAACAGGAAA GTGAACTGTC TCAAGGGAAG 
CCCAAGAGCA AAAAGCTCTT AAACGCTAAG AAAGAAATCC CAACAGATGT GAAAGGCAGT 
TCCTTTGAAC TACCGTACAC TCCCTCACTG GAAAATAAAC AGCGTAGAAA TCTCCCAACC 
AAGATTCCTC TTCCAACTAC ACTAGCAAGT GGATCCAAAT CACGAAATCC CCCGAAAACA 
AAAGGTACAA ATAAGTTAGT GGAAAACAGG CCAGTTGCCC TCTCAAAGTT CCTCCCCAGC 
AGTCAAGAAC TAGGTAATAC CAGCAGCTCA GAGGGTGAAA AGGATTCTCC TCCACCAGAA 
TGGGATGCTG TGCCAGTTCA CAAGCCCAGC AGCTCTACTG ACAGTCTTTA TAAACTGTCT 
CTGCAAACCC TCAATGCAGA CATTTTCTTA AAACAACGCC AGACCTCGCC AACACCTGCC 
TCTCCTTCTC TGCCCACCGC TCCCTGCCCG TTCACATCTC GTGGCAGCTA CAGCAGCGTT 
GTCAACAGCT CCGGCAGTGA CACAAAAGCC AAGCAGACGA GCAGCAGCAA GAGCAAGCTG 
ACAAAGGCGG CCTCGCTCCC CGGCAAGAAT GGCAACCCTA CCTTCGCCGC CGTCGCTGCA 
GGCTATGACA AGAGCCCGGG TGGGAATGGA TTTGCTAAAA TTTCTTCAAA CAAATCAGAT 
TTTTCCAGCA GCCTTGGCAT TTCACACATT CCTGTTGACA GCGATGGCTC TGACAGCTCG 
GGTTTGTGGA GTCCTGTCAG CAACCCCAAC AGCCCTGACT TCACCCCCCT CAATTCGTTC 
TCAGCCTTTG GAAATTCTTT TAATCTAACA GGTGTTTTCA GCAAACTGTC GCGATCCTGC 
AGTCAGTCGT CCCAGAGAAG TTGGAATGAG TTCAACAGCG GCCCCTCCTA CCTTTGGGAT 
TCTCCAGCCA CAGACCCCAG CCCTTCCTGG CCGGCCAGTT CCAGCTCCCC AACCCACACA 
GCCACTTCCA TCCTTGGCAA CAGCAGCGGC TTGTGGTCCA CCACTCCATT CAGCAGCTCC 
ATCTGGTCCA GCAACATCAA CAGCAACCTT CCTTTCTCCA CTCCAACAAA CGCGCTGTCG 
AGCATCAGCC TCATGGGCAC GGAAAACTCT GCCGCCGCCC ACACCCCTTC CGCCTCTGGC 
CCAGCTGACG ACTTGGGACA GACCTACAAC CCCTGGCGGA TCTGGAGCCC CACAGTCGGA 
AGAAGAAGCT CTGACCCTTG GTCTAATTCG CACTTCCCTC ATGAAAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496213.1
CDS	508..6138
Translation	MGKRAGGAAAAAAAASTSSAAGLEPAAGRGGGPRSAAAGLLGALHLVMTLVVAAARAEKEAFIQSESIIEVLRFDDGGLLQTETTLGLGSYQQKSISLYRGNCRPIRFEPPMLDFHEQPVGMPKMEKVYLHNPSSEETITLVSISATTSHFHASFFQNRKILPGGNTSFDVVFLARVVGNVENTLFINTSNHGVFTYQVFGVGVPNPYRLRPFLGARVPVNSSFSPIINIHNPHSEPLQVVEMYSSGGDLHLELPTGQQGGTRKLWEIPPYETKGVMRASFSSREADNHTAFIRIKTNASDSTEFIILPVEVEVTTAPGIYSSTEMLDFGTLRTQDLPKVLNLHLLNSGTKDVPITSVRPTPQNDAITVHFKPVTLKASESKYTKVASISFDASRAKKPSQFSGKITVKAKEKSYSKLEIPYQAEVLDGYLGFDHAATLFHIQDSPADPVERPIYLTNTFSFAILIHDVLLPEEARIMFQVHNFSQPVLILPNESGYIFTLFFMPSTSSMHIDNNILLVTNASKFHLPVRVYTGFLDYFVLPPKIEERFIDFGVLSATEASSILFAIINSNPIELAIKSWHIIGDGLSIELVATERGNRSTVIASLPELERSSLPDQSPVTLASGHFAVFRVKLTAKKLEGVHDGAIQITTDYEILTIPVKAVIAVGSLTCFPKHMVLPPSFPGKIVHQSLNIMNSFSQKVKIQQIRSLSEDVRFYYKRLRGNREDLEPGKKSKIANIYFDPGLQCGDHCYIGLPFLSKSEPKVQPGVAMQEDLWDADWDAHQSLFKAWMGIKENAGHRLNAMFEVNTDLQKNIVSKVSAELSWPSVLSSPRLLKFPLTNTNCSSEEEISLENPADVPVYVQFIPLALYSNPSVFADKLVSRFNLSKVAKLDLRTLEFQVYRNSAHPLQSPTGFTEGLSRHFILNLILKPGEKKSVKVKFTPLHNRTVSSLIIVRNNLTVMDAVMVQGQGTTENLRVAGKLPGPGSSLRFKITEALLKDCIDRLKLREPNFTLKRTFKVENTGQLEIRVETIEISGYACEGYGFKVVNCQEFALSANASRDIVILFTPDFTASRVIRELKFVTSSGSEFVFVLNASLPYHMLAACAEALPRPNWELALYIIISGVMSALFLLVIGTAYLEAQGIWEPFRRRLSFEASNPPFDVGRPFDLRRIVGISSEGNLNTLGCEHSHGRGFYSNASSRPGTGSHRQCGTSVHPHSSHGSKNSADVDNVRTRNSSSMSSRTSPQAAASQSTSKTSPLVSETAAATQGHTASRKSRGAKQGQHSSQHHSHSHSPLEQHSQPPPPVPQHQEPPPERLSPAPLTHPSHPERASTTRHSSEDSDITSLIEAMDKDFDHHDSSPLDVFTEQPPSPMSKSKGKGKSLQQRKAKPPKKQEEKEKRGKGKPQEDELKDALADDDSSSTTTETSNPDTEPLLREDTEKHKGRPAVPEKQESELSQGKPKSKKLLNAKKEIPTDVKGSSFELPYTPSLENKQRRNLPTKIPLPTTLASGSKSRNPPKTKGTNKLVENRPVALSKFLPSSQELGNTSSSEGEKDSPPPEWDAVPVHKPSSSTDSLYKLSLQTLNADIFLKQRQTSPTPASPSLPTAPCPFTSRGSYSSVVNSSGSDTKAKQTSSSKSKLTKAASLPGKNGNPTFAAVAAGYDKSPGGNGFAKISSNKSDFSSSLGISHIPVDSDGSDSSGLWSPVSNPNSPDFTPLNSFSAFGNSFNLTGVFSKLSRSCSQSSQRSWNEFNSGPSYLWDSPATDPSPSWPASSSSPTHTATSILGNSSGLWSTTPFSSSIWSSNINSNLPFSTPTNALSSISLMGTENSAAAHTPSASGPADDLGQTYNPWRIWSPTVGRRSSDPWSNSHFPHEN

Target ORF information:

RefSeq Version	XM_006496150.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane protein 131 (Tmem131), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496150.2

ORF Insert Sequence:

ATGGGGAAGC GGGCGGGAGG AGCAGCCGCC GCCGCCGCCG CCGCCTCCAC GTCCTCCGCG 
GCCGGGCTGG AGCCCGCGGC TGGCCGAGGC GGGGGTCCTC GGAGCGCAGC CGCGGGCCTC 
CTGGGAGCGC TGCACCTGGT GATGACCCTC GTGGTGGCCG CGGCGCGGGC CGAGAAGGAA 
GCATTCATTC AGTCGGAGAG CATAATAGAA GTCCTGCGCT TTGACGACGG AGGGCTACTA 
CAGACTGAGA CAACACTTGG CCTCGGTTCA TATCAACAGA AAAGCATTTC TCTGTACCGG 
GGTAATTGCA GGCCCATACG ATTCGAACCG CCCATGCTGG ATTTCCATGA GCAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTCTACTTA CATAACCCTA GTTCTGAAGA AACGATTACT 
TTAGTATCAA TATCTGCTAC CACATCACAT TTCCATGCAT CGTTTTTTCA AAATAGGAAA 
ATTCTTCCAG GAGGAAACAC ATCATTTGAT GTAGTTTTTC TTGCAAGAGT AGTAGGAAAT 
GTAGAAAATA CTTTATTTAT TAACACATCT AACCATGGGG TGTTCACTTA CCAGGTATTT 
GGTGTTGGGG TTCCAAATCC GTATCGATTA AGGCCATTTC TTGGGGCCAG AGTCCCCGTA 
AACAGCAGCT TTTCACCCAT AATAAACATA CACAATCCTC ACAGTGAGCC TTTACAGGTC 
GTAGAGATGT ACTCGAGTGG AGGTGACCTT CACCTAGAGC TCCCAACAGG TCAGCAAGGA 
GGCACCAGGA AGCTGTGGGA AATCCCTCCT TATGAAACCA AGGGAGTGAT GAGAGCCAGC 
TTTTCCTCCA GAGAAGCAGA CAATCACACA GCCTTCATCC GGATAAAGAC GAATGCATCA 
GACAGCACCG AGTTCATCAT TCTCCCGGTT GAGGTTGAAG TTACAACAGC TCCTGGAATC 
TATTCCTCAA CTGAAATGTT AGATTTTGGA ACACTTAGAA CACAAGATCT ACCAAAGGTT 
TTAAATCTTC ATTTACTAAA TTCAGGAACA AAGGATGTAC CAATAACAAG TGTTCGACCA 
ACACCACAAA ATGACGCTAT CACGGTACAT TTTAAACCAG TTACGTTAAA AGCTTCTGAA 
AGTAAATATA CCAAGGTTGC CAGCATTAGT TTTGATGCTT CGAGAGCAAA AAAGCCATCT 
CAATTTTCTG GGAAAATAAC TGTTAAAGCA AAGGAAAAGA GTTATTCTAA ACTCGAAATC 
CCCTACCAAG CAGAAGTTCT AGATGGTTAT TTGGGGTTTG ATCACGCCGC CACGCTGTTT 
CACATCCAGG ACAGTCCTGC TGACCCAGTG GAGAGGCCGA TCTACCTGAC GAACACTTTC 
AGTTTTGCGA TCCTCATTCA TGATGTGCTG TTACCAGAAG AAGCCAGAAT AATGTTTCAG 
GTGCACAACT TCAGCCAGCC GGTTCTCATT CTTCCTAATG AGTCGGGATA CATTTTTACC 
CTGTTTTTTA TGCCATCCAC CTCATCCATG CACATAGACA ACAACATTTT ACTTGTTACC 
AATGCTTCTA AGTTTCATTT ACCTGTGCGG GTATACACAG GCTTTTTAGA TTACTTTGTA 
TTGCCCCCCA AAATAGAAGA ACGCTTCATA GATTTTGGAG TACTGAGTGC TACAGAAGCA 
AGTAGTATTT TATTTGCAAT TATAAACAGC AATCCAATTG AGCTGGCAAT AAAAAGTTGG 
CATATCATAG GAGATGGCTT ATCAATAGAA CTTGTAGCTA CAGAGAGAGG CAACAGAAGC 
ACGGTGATTG CAAGCCTTCC AGAACTGGAG CGGTCCTCTT TGCCCGACCA GTCCCCGGTA 
ACATTAGCTT CAGGACACTT TGCAGTGTTC AGAGTCAAAC TTACTGCCAA GAAGCTAGAA 
GGGGTCCATG ATGGGGCCAT ACAGATCACA ACCGACTACG AGATCCTGAC CATCCCTGTG 
AAAGCTGTGA TCGCCGTGGG CTCGCTGACC TGCTTCCCCA AGCACATGGT TCTTCCCCCT 
TCCTTCCCAG GGAAAATAGT TCATCAGAGC TTAAATATTA TGAATTCCTT CTCACAGAAG 
GTGAAGATAC AGCAAATAAG GTCTTTGTCA GAAGACGTAC GATTCTACTA CAAACGATTA 
CGGGGTAATA GGGAAGACTT GGAGCCAGGG AAGAAGTCTA AGATTGCAAA CATTTATTTT 
GACCCTGGGC TGCAGTGTGG GGATCACTGC TATATCGGCT TGCCTTTCCT ATCCAAATCT 
GAACCCAAAG TACAGCCTGG GGTGGCCATG CAGGAGGACT TGTGGGATGC CGACTGGGAC 
GCACATCAGA GCTTGTTCAA GGCTTGGATG GGGATAAAGG AAAACGCAGG ACATAGGTTG 
AATGCTATGT TTGAAGTAAA TACAGATCTT CAGAAAAATA TCGTATCAAA AGTCAGTGCC 
GAGCTCTCCT GGCCGTCTGT CCTTAGCTCG CCCCGGCTCC TGAAGTTCCC ACTCACTAAC 
ACAAACTGTT CTTCGGAAGA AGAGATTAGC TTAGAAAACC CTGCAGACGT CCCTGTCTAC 
GTCCAGTTCA TCCCACTGGC TTTGTATTCC AACCCCTCCG TCTTTGCAGA TAAGTTAGTC 
TCAAGGTTTA ACTTGAGTAA GGTGGCCAAG CTAGATTTGA GAACACTTGA ATTCCAAGTC 
TACAGGAACA GCGCTCATCC ACTGCAAAGT CCAACAGGAT TTACAGAGGG TCTCTCTCGG 
CATTTCATTT TAAACCTCAT TTTAAAACCT GGAGAAAAGA AATCTGTCAA AGTAAAGTTC 
ACTCCACTCC ACAACAGAAC TGTGTCTTCC CTGATCATAG TCAGAAACAA CCTGACTGTG 
ATGGACGCTG TGATGGTTCA AGGTCAAGGG ACAACGGAGA ACTTGAGGGT GGCAGGCAAG 
CTTCCAGGTC CAGGAAGCTC CTTACGCTTC AAAATCACAG AAGCATTGTT AAAGGATTGC 
ATAGACCGTT TGAAACTAAG AGAACCAAAT TTCACATTGA AAAGAACATT CAAAGTAGAG 
AATACGGGAC AACTTGAAAT CCGTGTAGAA ACCATTGAGA TCAGTGGATA TGCGTGTGAG 
GGATATGGTT TTAAAGTGGT TAACTGTCAA GAGTTTGCCC TGAGTGCCAA CGCCTCCAGA 
GACATAGTCA TATTGTTTAC TCCAGATTTC ACAGCCTCCA GAGTCATTCG GGAGCTGAAG 
TTTGTGACAA GCAGTGGCTC CGAGTTTGTG TTTGTGTTGA ATGCCTCTCT TCCGTACCAC 
ATGCTAGCCG CCTGTGCAGA AGCCCTCCCT AGACCCAACT GGGAGCTCGC GCTCTACATC 
ATCATCTCCG GGGTCATGAG TGCACTCTTT CTCCTGGTCA TTGGAACAGC CTACTTGGAA 
GCTCAAGGGA TTTGGGAGCC CTTCCGAAGG CGACTCTCCT TTGAAGCCTC AAACCCGCCC 
TTTGATGTAG GAAGGCCATT TGATCTCAGG AGAATCGTTG GTATTTCATC TGAAGGAAAC 
TTGAACACAC TTGGCTGCGA ACACAGTCAT GGTAGGGGTT TCTATAGTAA CGCATCATCC 
CGGCCTGGTA CAGGCAGTCA CAGGCAGTGT GGCACATCAG TCCACCCACA CAGCAGCCAT 
GGCAGCAAAA ACTCAGCTGA TGTGGACAAC GTCAGAACCA GAAACAGTTC AAGTATGTCA 
AGCAGGACTT CTCCACAAGC AGCTGCCTCA CAGTCTACAA GCAAAACCAG CCCCCTAGTC 
TCAGAGACAG CTGCAGCAAC CCAGGGTCAC ACAGCAAGCA GGAAGTCCAG AGGGGCAAAG 
CAGGGCCAGC ACAGCAGCCA GCACCACAGC CACAGCCACA GCCCACTGGA GCAGCACTCA 
CAGCCTCCAC CACCAGTGCC TCAGCACCAG GAGCCACCAC CCGAAAGGCT GTCTCCTGCC 
CCCCTCACAC ACCCTTCCCA CCCCGAGCGA GCCAGCACCA CACGGCACAG CTCAGAGGAC 
TCCGACATCA CCAGTCTCAT AGAAGCCATG GACAAAGACT TTGACCACCA CGACTCCTCC 
CCCCTAGACG TGTTTACAGA GCAGCCTCCG TCACCGATGT CAAAAAGCAA AGGGAAAGGA 
AAGTCTCTCC AACAACGTAA GGCCAAGCCT CCGAAGAAGC AGGAGGAGAA GGAGAAGAGG 
GGGAAGGGGA AGCCGCAGGA GGATGAGCTG AAGGACGCTC TGGCCGACGA CGACAGCTCC 
TCCACCACCA CAGAGACCTC CAACCCCGAC ACAGAGCCAC TCCTCAGGGA GGATACAGAG 
AAGCACAAGG GGAGACCAGC CGTGCCTGAA AAACAGGAAA GTGAACTGTC TCAAGGGAAG 
CCCAAGAGCA AAAAGCTCTT AAACGCTAAG AAAGAAATCC CAACAGATGT GAAAGGCAGT 
TCCTTTGAAC TACCGTACAC TCCCTCACTG GAAAATAAAC AGCGTAGAAA TCTCCCAACC 
AAGATTCCTC TTCCAACTAC ACTAGCAAGT GGATCCAAAT CACGAAATCC CCCGAAAACA 
AAAGGTACAA ATAAGTTAGT GGAAAACAGG CCAGTTGCCC TCTCAAAGTT CCTCCCCAGC 
AGTCAAGAAC TAGGTAATAC CAGCAGCTCA GAGGGTGAAA AGGATTCTCC TCCACCAGAA 
TGGGATGCTG TGCCAGTTCA CAAGCCCAGC AGCTCTACTG ACAGTCTTTA TAAACTGTCT 
CTGCAAACCC TCAATGCAGA CATTTTCTTA AAACAACGCC AGACCTCGCC AACACCTGCC 
TCTCCTTCTC TGCCCACCGC TCCCTGCCCG TTCACATCTC GTGGCAGCTA CAGCAGCGTT 
GTCAACAGCT CCGGCAGTGA CACAAAAGCC AAGCAGACGA GCAGCAGCAA GAGCAAGCTG 
ACAAAGGCGG CCTCGCTCCC CGGCAAGAAT GGCAACCCTA CCTTCGCCGC CGTCGCTGCA 
GGCTATGACA AGAGCCCGGG TGGGAATGGA TTTGCTAAAA TTTCTTCAAA CAAATCAGAT 
TTTTCCAGCA GCCTTGGCAT TTCACACATT CCTGTTGACA GCGATGGCTC TGACAGCTCG 
GGTTTGTGGA GTCCTGTCAG CAACCCCAAC AGCCCTGACT TCACCCCCCT CAATTCGTTC 
TCAGCCTTTG GAAATTCTTT TAATCTAACA GGTGTTTTCA GCAAACTGTC GCGATCCTGC 
AGTCAGTCGT CCCAGAGAAG TTGGAATGAG TTCAACAGCG GCCCCTCCTA CCTTTGGGAT 
TCTCCAGCCA CAGACCCCAG CCCTTCCTGG CCGGCCAGTT CCAGCTCCCC AACCCACACA 
GCCACTTCCA TCCTTGGCAA CAGCAGCGGC TTGTGGTCCA CCACTCCATT CAGCAGCTCC 
ATCTGGTCCA GCAACATCAA CAGCAACCTT CCTTTCTCCA CTCCAACAAA CGCGCTGTCG 
AGCATCAGCC TCATGGGCAC GGAAAACTCT GCCGCCGCCC ACACCCCTTC CGCCTCTGGC 
CCAGCTGACG ACTTGGGACA GACCTACAAC CCCTGGCGGA TCTGGAGCCC CACAGTCGGA 
AGAAGAAGCT CTGACCCTTG GTCTAATTCG CACTTCCCTC ATGAAAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmem131 ( XM_006496152.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496215.1 Mus musculus transmembrane protein 131 (Tmem131), transcript variant X4, mRNA.

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CloneID	OMu27536
Clone ID Related Accession (Same CDS sequence)	XM_006496152.2 , XM_006496152.3
Accession Version	XM_006496152.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	transmembrane protein 131 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496152.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGC GGGCGGGAGG AGCAGCCGCC GCCGCCGCCG CCGCCTCCAC GTCCTCCGCG 
GCCGGGCTGG AGCCCGCGGC TGGCCGAGGC GGGGGTCCTC GGAGCGCAGC CGCGGGCCTC 
CTGGGAGCGC TGCACCTGGT GATGACCCTC GTGGTGGCCG CGGCGCGGGC CGAGAAGGAA 
GCATTCATTC AGTCGGAGAG CATAATAGAA GTCCTGCGCT TTGACGACGG AGGGCTACTA 
CAGACTGAGA CAACACTTGG CCTCGGTTCA TATCAACAGA AAAGCATTTC TCTGTACCGG 
GGTAATTGCA GGCCCATACG ATTCGAACCG CCCATGCTGG ATTTCCATGA GCAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTCTACTTA CATAACCCTA GTTCTGAAGA AACGATTACT 
TTAGTATCAA TATCTGCTAC CACATCACAT TTCCATGCAT CGTTTTTTCA AAATAGGAAA 
ATTCTTCCAG GAGGAAACAC ATCATTTGAT GTAGTTTTTC TTGCAAGAGT AGTAGGAAAT 
GTAGAAAATA CTTTATTTAT TAACACATCT AACCATGGGG TGTTCACTTA CCAGGTATTT 
GGTGTTGGGG TTCCAAATCC GTATCGATTA AGGCCATTTC TTGGGGCCAG AGTCCCCGTA 
AACAGCAGCT TTTCACCCAT AATAAACATA CACAATCCTC ACAGTGAGCC TTTACAGGTC 
GTAGAGATGT ACTCGAGTGG AGGTGACCTT CACCTAGAGC TCCCAACAGG TCAGCAAGGA 
GGCACCAGGA AGCTGTGGGA AATCCCTCCT TATGAAACCA AGGGAGTGAT GAGAGCCAGC 
TTTTCCTCCA GAGAAGCAGA CAATCACACA GCCTTCATCC GGATAAAGAC GAATGCATCA 
GACAGCACCG AGTTCATCAT TCTCCCGGTT GAGGTTGAAG TTACAACAGC TCCTGGAATC 
TATTCCTCAA CTGAAATGTT AGATTTTGGA ACACTTAGAA CACAAGATCT ACCAAAGGTT 
TTAAATCTTC ATTTACTAAA TTCAGGAACA AAGGATGTAC CAATAACAAG TGTTCGACCA 
ACACCACAAA ATGACGCTAT CACGGTACAT TTTAAACCAG TTACGTTAAA AGCTTCTGAA 
AGTAAATATA CCAAGGTTGC CAGCATTAGT TTTGATGCTT CGAGAGCAAA AAAGCCATCT 
CAATTTTCTG GGAAAATAAC TGTTAAAGCA AAGGAAAAGA GTTATTCTAA ACTCGAAATC 
CCCTACCAAG CAGAAGTTCT AGATGGTTAT TTGGGGTTTG ATCACGCCGC CACGCTGTTT 
CACATCCAGG ACAGTCCTGC TGACCCAGTG GAGAGGCCGA TCTACCTGAC GAACACTTTC 
AGTTTTGCGA TCCTCATTCA TGATGTGCTG TTACCAGAAG AAGCCAGAAT AATGTTTCAG 
GTGCACAACT TCAGCCAGCC GGTTCTCATT CTTCCTAATG AGTCGGGATA CATTTTTACC 
CTGTTTTTTA TGCCATCCAC CTCATCCATG CACATAGACA ACAACATTTT ACTTGTTACC 
AATGCTTCTA AGTTTCATTT ACCTGTGCGG GTATACACAG GCTTTTTAGA TTACTTTGTA 
TTGCCCCCCA AAATAGAAGA ACGCTTCATA GATTTTGGAG TACTGAGTGC TACAGAAGCA 
AGTAGTATTT TATTTGCAAT TATAAACAGC AATCCAATTG AGCTGGCAAT AAAAAGTTGG 
CATATCATAG GAGATGGCTT ATCAATAGAA CTTGTAGCTA CAGAGAGAGG CAACAGAAGC 
ACGGTGATTG CAAGCCTTCC AGAACTGGAG CGGTCCTCTT TGCCCGACCA GTCCCCGGTA 
ACATTAGCTT CAGGACACTT TGCAGTGTTC AGAGTCAAAC TTACTGCCAA GAAGCTAGAA 
GGGGTCCATG ATGGGGCCAT ACAGATCACA ACCGACTACG AGATCCTGAC CATCCCTGTG 
AAAGCTGTGA TCGCCGTGGG CTCGCTGACC TGCTTCCCCA AGCACATGGT TCTTCCCCCT 
TCCTTCCCAG GGAAAATAGT TCATCAGAGC TTAAATATTA TGAATTCCTT CTCACAGAAG 
GTGAAGATAC AGCAAATAAG GTCTTTGTCA GAAGACGTAC GATTCTACTA CAAACGATTA 
CGGGGTAATA GGGAAGACTT GGAGCCAGGG AAGAAGTCTA AGATTGCAAA CATTTATTTT 
GACCCTGGGC TGCAGTGTGG GGATCACTGC TATATCGGCT TGCCTTTCCT ATCCAAATCT 
GAACCCAAAG TACAGCCTGG GGTGGCCATG CAGGAGGACT TGTGGGATGC CGACTGGGAC 
GCACATCAGA GCTTGTTCAA GGCTTGGATG GGGATAAAGG AAAACGCAGG ACATAGGTTG 
AATGCTATGT TTGAAGTAAA TACAGATCTT CAGAAAAATA TCGTATCAAA AGTCAGTGCC 
GAGCTCTCCT GGCCGTCTGT CCTTAGCTCG CCCCGGCTCC TGAAGTTCCC ACTCACTAAC 
ACAAACTGTT CTTCGGAAGA AGAGATTAGC TTAGAAAACC CTGCAGACGT CCCTGTCTAC 
GTCCAGTTCA TCCCACTGGC TTTGTATTCC AACCCCTCCG TCTTTGCAGA TAAGTTAGTC 
TCAAGGTTTA ACTTGAGTAA GGTGGCCAAG CTAGATTTGA GAACACTTGA ATTCCAAGTC 
TACAGGAACA GCGCTCATCC ACTGCAAAGT CCAACAGGAT TTACAGAGGG TCTCTCTCGG 
CATTTCATTT TAAACCTCAT TTTAAAACCT GGAGAAAAGA AATCTGTCAA AGTAAAGTTC 
ACTCCACTCC ACAACAGAAC TGTGTCTTCC CTGATCATAG TCAGAAACAA CCTGACTGTG 
ATGGACGCTG TGATGGTTCA AGGTCAAGGG ACAACGGAGA ACTTGAGGGT GGCAGGCAAG 
CTTCCAGGTC CAGGAAGCTC CTTACGCTTC AAAATCACAG AAGCATTGTT AAAGGATTGC 
ATAGACCGTT TGAAACTAAG AGAACCAAAT TTCACATTGA AAAGAACATT CAAAGTAGAG 
AATACGGGAC AACTTGAAAT CCGTGTAGAA ACCATTGAGA TCAGTGGATA TGCGTGTGAG 
GGATATGGTT TTAAAGTGGT TAACTGTCAA GAGTTTGCCC TGAGTGCCAA CGCCTCCAGA 
GACATAGTCA TATTGTTTAC TCCAGATTTC ACAGCCTCCA GAGTCATTCG GGAGCTGAAG 
TTTGTGACAA GCAGTGGCTC CGAGTTTGTG TTTGTGTTGA ATGCCTCTCT TCCGTACCAC 
ATGCTAGCCG CCTGTGCAGA AGCCCTCCCT AGACCCAACT GGGAGCTCGC GCTCTACATC 
ATCATCTCCG GGGTCATGAG TGCACTCTTT CTCCTGGTCA TTGGAACAGC CTACTTGGAA 
GCTCAAGGGA TTTGGGAGCC CTTCCGAAGG CGACTCTCCT TTGAAGCCTC AAACCCGCCC 
TTTGATGTAG GAAGGCCATT TGATCTCAGG AGAATCGTTG GTATTTCATC TGAAGGAAAC 
TTGAACACAC TTGGCTGCGA ACACAGTCAT GGTAGGGGTT TCTATAGTAA CGCATCATCC 
CGGCCTGGTA CAGGCAGTCA CAGGCAGTGT GGCACATCAG TCCACCCACA CAGCAGCCAT 
GGCAGCAAAA ACTCAGCTGA TGTGGACAAC GTCAGAACCA GAAACAGTTC AAGTATGTCA 
AGCAGGACTT CTCCACAAGC AGCTGCCTCA CAGTCTACAA GCAAAACCAG CCCCCTAGTC 
TCAGAGACAG CTGCAGCAAC CCAGGGTCAC ACAGCAAGCA GGAAGTCCAG AGGGGCAAAG 
CAGGGCCAGC ACAGCAGCCA GCACCACAGC CACAGCCACA GCCCACTGGA GCAGCACTCA 
CAGCCTCCAC CACCAGTGCC TCAGCACCAG GAGCCACCAC CCGAAAGGCT GTCTCCTGCC 
CCCCTCACAC ACCCTTCCCA CCCCGAGCGA GCCAGCACCA CACGGCACAG CTCAGAGGAC 
TCCGACATCA CCAGTCTCAT AGAAGCCATG GACAAAGACT TTGACCACCA CGACTCCTCC 
CCCCTAGACG TGTTTACAGA GCAGCCTCCG TCACCGATGT CAAAAAGCAA AGGGAAAGGA 
AAGTCTCTCC AACAACGTAA GGCCAAGCCT CCGAAGAAGC AGGAGGAGAA GGAGAAGAGG 
GGGAAGGGGA AGCCGCAGGA GGATGAGCTG AAGGACGCTC TGGCCGACGA CGACAGCTCC 
TCCACCACCA CAGAGACCTC CAACCCCGAC ACAGAGCCAC TCCTCAGGGA GGATACAGAG 
AAGCACAAGG GGAGACCAGC CGTGCCTGAA AAACAGGAAA GTGAACTGTC TCAAGGGAAG 
CCCAAGAGCA AAAAGCTCTT AAACGCTAAG AAAGAAATCC CAACAGATGT GAAAGGCAGT 
TCCTTTGAAC TACCGTACAC TCCCTCACTG GAAAATAAAC AGCGTAGAAA TCTCCCAACC 
AAGATTCCTC TTCCAACTAC ACTAGCAAGT GGATCCAAAT CACGAAATCC CCCGAAAACA 
AAAGGTACAA ATAAGTTAGT GGAAAACAGG CCAGTTGCCC TCTCAAAGTT CCTCCCCAGC 
AGTCAAGAAC TAGGTAATAC CAGCAGCTCA GAGGGTGAAA AGGATTCTCC TCCACCAGAA 
TGGGATGCTG TGCCAGTTCA CAAGCCCAGC AGCTCTACTG ACAGTCTTTA TAAACTGTCT 
CTGCAAACCC TCAATGCAGA CATTTTCTTA AAACAACGCC AGACCTCGCC AACACCTGCC 
TCTCCTTCTC TGCCCACCGC TCCCTGCCCG TTCACATCTC GTGGCAGCTA CAGCAGCGTT 
GTCAACAGCT CCGGCAGGTA CCTGACCCCA GCACTGACCT TATCCTGTCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496215.1
CDS	530..5443
Translation	MGKRAGGAAAAAAAASTSSAAGLEPAAGRGGGPRSAAAGLLGALHLVMTLVVAAARAEKEAFIQSESIIEVLRFDDGGLLQTETTLGLGSYQQKSISLYRGNCRPIRFEPPMLDFHEQPVGMPKMEKVYLHNPSSEETITLVSISATTSHFHASFFQNRKILPGGNTSFDVVFLARVVGNVENTLFINTSNHGVFTYQVFGVGVPNPYRLRPFLGARVPVNSSFSPIINIHNPHSEPLQVVEMYSSGGDLHLELPTGQQGGTRKLWEIPPYETKGVMRASFSSREADNHTAFIRIKTNASDSTEFIILPVEVEVTTAPGIYSSTEMLDFGTLRTQDLPKVLNLHLLNSGTKDVPITSVRPTPQNDAITVHFKPVTLKASESKYTKVASISFDASRAKKPSQFSGKITVKAKEKSYSKLEIPYQAEVLDGYLGFDHAATLFHIQDSPADPVERPIYLTNTFSFAILIHDVLLPEEARIMFQVHNFSQPVLILPNESGYIFTLFFMPSTSSMHIDNNILLVTNASKFHLPVRVYTGFLDYFVLPPKIEERFIDFGVLSATEASSILFAIINSNPIELAIKSWHIIGDGLSIELVATERGNRSTVIASLPELERSSLPDQSPVTLASGHFAVFRVKLTAKKLEGVHDGAIQITTDYEILTIPVKAVIAVGSLTCFPKHMVLPPSFPGKIVHQSLNIMNSFSQKVKIQQIRSLSEDVRFYYKRLRGNREDLEPGKKSKIANIYFDPGLQCGDHCYIGLPFLSKSEPKVQPGVAMQEDLWDADWDAHQSLFKAWMGIKENAGHRLNAMFEVNTDLQKNIVSKVSAELSWPSVLSSPRLLKFPLTNTNCSSEEEISLENPADVPVYVQFIPLALYSNPSVFADKLVSRFNLSKVAKLDLRTLEFQVYRNSAHPLQSPTGFTEGLSRHFILNLILKPGEKKSVKVKFTPLHNRTVSSLIIVRNNLTVMDAVMVQGQGTTENLRVAGKLPGPGSSLRFKITEALLKDCIDRLKLREPNFTLKRTFKVENTGQLEIRVETIEISGYACEGYGFKVVNCQEFALSANASRDIVILFTPDFTASRVIRELKFVTSSGSEFVFVLNASLPYHMLAACAEALPRPNWELALYIIISGVMSALFLLVIGTAYLEAQGIWEPFRRRLSFEASNPPFDVGRPFDLRRIVGISSEGNLNTLGCEHSHGRGFYSNASSRPGTGSHRQCGTSVHPHSSHGSKNSADVDNVRTRNSSSMSSRTSPQAAASQSTSKTSPLVSETAAATQGHTASRKSRGAKQGQHSSQHHSHSHSPLEQHSQPPPPVPQHQEPPPERLSPAPLTHPSHPERASTTRHSSEDSDITSLIEAMDKDFDHHDSSPLDVFTEQPPSPMSKSKGKGKSLQQRKAKPPKKQEEKEKRGKGKPQEDELKDALADDDSSSTTTETSNPDTEPLLREDTEKHKGRPAVPEKQESELSQGKPKSKKLLNAKKEIPTDVKGSSFELPYTPSLENKQRRNLPTKIPLPTTLASGSKSRNPPKTKGTNKLVENRPVALSKFLPSSQELGNTSSSEGEKDSPPPEWDAVPVHKPSSSTDSLYKLSLQTLNADIFLKQRQTSPTPASPSLPTAPCPFTSRGSYSSVVNSSGRYLTPALTLSCL

Target ORF information:

RefSeq Version	XM_006496152.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane protein 131 (Tmem131), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496152.2

ORF Insert Sequence:

ATGGGGAAGC GGGCGGGAGG AGCAGCCGCC GCCGCCGCCG CCGCCTCCAC GTCCTCCGCG 
GCCGGGCTGG AGCCCGCGGC TGGCCGAGGC GGGGGTCCTC GGAGCGCAGC CGCGGGCCTC 
CTGGGAGCGC TGCACCTGGT GATGACCCTC GTGGTGGCCG CGGCGCGGGC CGAGAAGGAA 
GCATTCATTC AGTCGGAGAG CATAATAGAA GTCCTGCGCT TTGACGACGG AGGGCTACTA 
CAGACTGAGA CAACACTTGG CCTCGGTTCA TATCAACAGA AAAGCATTTC TCTGTACCGG 
GGTAATTGCA GGCCCATACG ATTCGAACCG CCCATGCTGG ATTTCCATGA GCAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTCTACTTA CATAACCCTA GTTCTGAAGA AACGATTACT 
TTAGTATCAA TATCTGCTAC CACATCACAT TTCCATGCAT CGTTTTTTCA AAATAGGAAA 
ATTCTTCCAG GAGGAAACAC ATCATTTGAT GTAGTTTTTC TTGCAAGAGT AGTAGGAAAT 
GTAGAAAATA CTTTATTTAT TAACACATCT AACCATGGGG TGTTCACTTA CCAGGTATTT 
GGTGTTGGGG TTCCAAATCC GTATCGATTA AGGCCATTTC TTGGGGCCAG AGTCCCCGTA 
AACAGCAGCT TTTCACCCAT AATAAACATA CACAATCCTC ACAGTGAGCC TTTACAGGTC 
GTAGAGATGT ACTCGAGTGG AGGTGACCTT CACCTAGAGC TCCCAACAGG TCAGCAAGGA 
GGCACCAGGA AGCTGTGGGA AATCCCTCCT TATGAAACCA AGGGAGTGAT GAGAGCCAGC 
TTTTCCTCCA GAGAAGCAGA CAATCACACA GCCTTCATCC GGATAAAGAC GAATGCATCA 
GACAGCACCG AGTTCATCAT TCTCCCGGTT GAGGTTGAAG TTACAACAGC TCCTGGAATC 
TATTCCTCAA CTGAAATGTT AGATTTTGGA ACACTTAGAA CACAAGATCT ACCAAAGGTT 
TTAAATCTTC ATTTACTAAA TTCAGGAACA AAGGATGTAC CAATAACAAG TGTTCGACCA 
ACACCACAAA ATGACGCTAT CACGGTACAT TTTAAACCAG TTACGTTAAA AGCTTCTGAA 
AGTAAATATA CCAAGGTTGC CAGCATTAGT TTTGATGCTT CGAGAGCAAA AAAGCCATCT 
CAATTTTCTG GGAAAATAAC TGTTAAAGCA AAGGAAAAGA GTTATTCTAA ACTCGAAATC 
CCCTACCAAG CAGAAGTTCT AGATGGTTAT TTGGGGTTTG ATCACGCCGC CACGCTGTTT 
CACATCCAGG ACAGTCCTGC TGACCCAGTG GAGAGGCCGA TCTACCTGAC GAACACTTTC 
AGTTTTGCGA TCCTCATTCA TGATGTGCTG TTACCAGAAG AAGCCAGAAT AATGTTTCAG 
GTGCACAACT TCAGCCAGCC GGTTCTCATT CTTCCTAATG AGTCGGGATA CATTTTTACC 
CTGTTTTTTA TGCCATCCAC CTCATCCATG CACATAGACA ACAACATTTT ACTTGTTACC 
AATGCTTCTA AGTTTCATTT ACCTGTGCGG GTATACACAG GCTTTTTAGA TTACTTTGTA 
TTGCCCCCCA AAATAGAAGA ACGCTTCATA GATTTTGGAG TACTGAGTGC TACAGAAGCA 
AGTAGTATTT TATTTGCAAT TATAAACAGC AATCCAATTG AGCTGGCAAT AAAAAGTTGG 
CATATCATAG GAGATGGCTT ATCAATAGAA CTTGTAGCTA CAGAGAGAGG CAACAGAAGC 
ACGGTGATTG CAAGCCTTCC AGAACTGGAG CGGTCCTCTT TGCCCGACCA GTCCCCGGTA 
ACATTAGCTT CAGGACACTT TGCAGTGTTC AGAGTCAAAC TTACTGCCAA GAAGCTAGAA 
GGGGTCCATG ATGGGGCCAT ACAGATCACA ACCGACTACG AGATCCTGAC CATCCCTGTG 
AAAGCTGTGA TCGCCGTGGG CTCGCTGACC TGCTTCCCCA AGCACATGGT TCTTCCCCCT 
TCCTTCCCAG GGAAAATAGT TCATCAGAGC TTAAATATTA TGAATTCCTT CTCACAGAAG 
GTGAAGATAC AGCAAATAAG GTCTTTGTCA GAAGACGTAC GATTCTACTA CAAACGATTA 
CGGGGTAATA GGGAAGACTT GGAGCCAGGG AAGAAGTCTA AGATTGCAAA CATTTATTTT 
GACCCTGGGC TGCAGTGTGG GGATCACTGC TATATCGGCT TGCCTTTCCT ATCCAAATCT 
GAACCCAAAG TACAGCCTGG GGTGGCCATG CAGGAGGACT TGTGGGATGC CGACTGGGAC 
GCACATCAGA GCTTGTTCAA GGCTTGGATG GGGATAAAGG AAAACGCAGG ACATAGGTTG 
AATGCTATGT TTGAAGTAAA TACAGATCTT CAGAAAAATA TCGTATCAAA AGTCAGTGCC 
GAGCTCTCCT GGCCGTCTGT CCTTAGCTCG CCCCGGCTCC TGAAGTTCCC ACTCACTAAC 
ACAAACTGTT CTTCGGAAGA AGAGATTAGC TTAGAAAACC CTGCAGACGT CCCTGTCTAC 
GTCCAGTTCA TCCCACTGGC TTTGTATTCC AACCCCTCCG TCTTTGCAGA TAAGTTAGTC 
TCAAGGTTTA ACTTGAGTAA GGTGGCCAAG CTAGATTTGA GAACACTTGA ATTCCAAGTC 
TACAGGAACA GCGCTCATCC ACTGCAAAGT CCAACAGGAT TTACAGAGGG TCTCTCTCGG 
CATTTCATTT TAAACCTCAT TTTAAAACCT GGAGAAAAGA AATCTGTCAA AGTAAAGTTC 
ACTCCACTCC ACAACAGAAC TGTGTCTTCC CTGATCATAG TCAGAAACAA CCTGACTGTG 
ATGGACGCTG TGATGGTTCA AGGTCAAGGG ACAACGGAGA ACTTGAGGGT GGCAGGCAAG 
CTTCCAGGTC CAGGAAGCTC CTTACGCTTC AAAATCACAG AAGCATTGTT AAAGGATTGC 
ATAGACCGTT TGAAACTAAG AGAACCAAAT TTCACATTGA AAAGAACATT CAAAGTAGAG 
AATACGGGAC AACTTGAAAT CCGTGTAGAA ACCATTGAGA TCAGTGGATA TGCGTGTGAG 
GGATATGGTT TTAAAGTGGT TAACTGTCAA GAGTTTGCCC TGAGTGCCAA CGCCTCCAGA 
GACATAGTCA TATTGTTTAC TCCAGATTTC ACAGCCTCCA GAGTCATTCG GGAGCTGAAG 
TTTGTGACAA GCAGTGGCTC CGAGTTTGTG TTTGTGTTGA ATGCCTCTCT TCCGTACCAC 
ATGCTAGCCG CCTGTGCAGA AGCCCTCCCT AGACCCAACT GGGAGCTCGC GCTCTACATC 
ATCATCTCCG GGGTCATGAG TGCACTCTTT CTCCTGGTCA TTGGAACAGC CTACTTGGAA 
GCTCAAGGGA TTTGGGAGCC CTTCCGAAGG CGACTCTCCT TTGAAGCCTC AAACCCGCCC 
TTTGATGTAG GAAGGCCATT TGATCTCAGG AGAATCGTTG GTATTTCATC TGAAGGAAAC 
TTGAACACAC TTGGCTGCGA ACACAGTCAT GGTAGGGGTT TCTATAGTAA CGCATCATCC 
CGGCCTGGTA CAGGCAGTCA CAGGCAGTGT GGCACATCAG TCCACCCACA CAGCAGCCAT 
GGCAGCAAAA ACTCAGCTGA TGTGGACAAC GTCAGAACCA GAAACAGTTC AAGTATGTCA 
AGCAGGACTT CTCCACAAGC AGCTGCCTCA CAGTCTACAA GCAAAACCAG CCCCCTAGTC 
TCAGAGACAG CTGCAGCAAC CCAGGGTCAC ACAGCAAGCA GGAAGTCCAG AGGGGCAAAG 
CAGGGCCAGC ACAGCAGCCA GCACCACAGC CACAGCCACA GCCCACTGGA GCAGCACTCA 
CAGCCTCCAC CACCAGTGCC TCAGCACCAG GAGCCACCAC CCGAAAGGCT GTCTCCTGCC 
CCCCTCACAC ACCCTTCCCA CCCCGAGCGA GCCAGCACCA CACGGCACAG CTCAGAGGAC 
TCCGACATCA CCAGTCTCAT AGAAGCCATG GACAAAGACT TTGACCACCA CGACTCCTCC 
CCCCTAGACG TGTTTACAGA GCAGCCTCCG TCACCGATGT CAAAAAGCAA AGGGAAAGGA 
AAGTCTCTCC AACAACGTAA GGCCAAGCCT CCGAAGAAGC AGGAGGAGAA GGAGAAGAGG 
GGGAAGGGGA AGCCGCAGGA GGATGAGCTG AAGGACGCTC TGGCCGACGA CGACAGCTCC 
TCCACCACCA CAGAGACCTC CAACCCCGAC ACAGAGCCAC TCCTCAGGGA GGATACAGAG 
AAGCACAAGG GGAGACCAGC CGTGCCTGAA AAACAGGAAA GTGAACTGTC TCAAGGGAAG 
CCCAAGAGCA AAAAGCTCTT AAACGCTAAG AAAGAAATCC CAACAGATGT GAAAGGCAGT 
TCCTTTGAAC TACCGTACAC TCCCTCACTG GAAAATAAAC AGCGTAGAAA TCTCCCAACC 
AAGATTCCTC TTCCAACTAC ACTAGCAAGT GGATCCAAAT CACGAAATCC CCCGAAAACA 
AAAGGTACAA ATAAGTTAGT GGAAAACAGG CCAGTTGCCC TCTCAAAGTT CCTCCCCAGC 
AGTCAAGAAC TAGGTAATAC CAGCAGCTCA GAGGGTGAAA AGGATTCTCC TCCACCAGAA 
TGGGATGCTG TGCCAGTTCA CAAGCCCAGC AGCTCTACTG ACAGTCTTTA TAAACTGTCT 
CTGCAAACCC TCAATGCAGA CATTTTCTTA AAACAACGCC AGACCTCGCC AACACCTGCC 
TCTCCTTCTC TGCCCACCGC TCCCTGCCCG TTCACATCTC GTGGCAGCTA CAGCAGCGTT 
GTCAACAGCT CCGGCAGGTA CCTGACCCCA GCACTGACCT TATCCTGTCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmem131 ( XM_017321814.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177303.1 Mus musculus transmembrane protein 131 (Tmem131), transcript variant X2, mRNA.

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CloneID	OMu77244
Clone ID Related Accession (Same CDS sequence)	XM_017321814.2 , XM_017321814.1
Accession Version	XM_017321814.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	transmembrane protein 131 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGC GGGCGGGAGG AGCAGCCGCC GCCGCCGCCG CCGCCTCCAC GTCCTCCGCG 
GCCGGGCTGG AGCCCGCGGC TGGCCGAGGC GGGGGTCCTC GGAGCGCAGC CGCGGGCCTC 
CTGGGAGCGC TGCACCTGGT GATGACCCTC GTGGTGGCCG CGGCGCGGGC CGAGAAGGAA 
GCATTCATTC AGTCGGAGAG CATAATAGAA GTCCTGCGCT TTGACGACGG AGGGCTACTA 
CAGACTGAGA CAACACTTGG CCTCGGTTCA TATCAACAGA AAAGCATTTC TCTGTACCGG 
GGTAATTGCA GGCCCATACG ATTCGAACCG CCCATGCTGG ATTTCCATGA GCAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTCTACTTA CATAACCCTA GTTCTGAAGA AACGATTACT 
TTAGTATCAA TATCTGCTAC CACATCACAT TTCCATGCAT CGTTTTTTCA AAATAGGAAA 
ATTCTTCCAG GAGGAAACAC ATCATTTGAT GTAGTTTTTC TTGCAAGAGT AGTAGGAAAT 
GTAGAAAATA CTTTATTTAT TAACACATCT AACCATGGGG TGTTCACTTA CCAGGTATTT 
GGTGTTGGGG TTCCAAATCC GTATCGATTA AGGCCATTTC TTGGGGCCAG AGTCCCCGTA 
AACAGCAGCT TTTCACCCAT AATAAACATA CACAATCCTC ACAGTGAGCC TTTACAGGTC 
GTAGAGATGT ACTCGAGTGG AGGTGACCTT CACCTAGAGC TCCCAACAGG TCAGCAAGGA 
GGCACCAGGA AGCTGTGGGA AATCCCTCCT TATGAAACCA AGGGAGTGAT GAGAGCCAGC 
TTTTCCTCCA GAGAAGCAGA CAATCACACA GCCTTCATCC GGATAAAGAC GAATGCATCA 
GACAGCACCG AGTTCATCAT TCTCCCGGTT GAGGTTGAAG TTACAACAGC TCCTGGAATC 
TATTCCTCAA CTGAAATGTT AGATTTTGGA ACACTTAGAA CACAAGATCT ACCAAAGGTT 
TTAAATCTTC ATTTACTAAA TTCAGGAACA AAGGATGTAC CAATAACAAG TGTTCGACCA 
ACACCACAAA ATGACGCTAT CACGGTACAT TTTAAACCAG TTACGTTAAA AGCTTCTGAA 
AGTAAATATA CCAAGGTTGC CAGCATTAGT TTTGATGCTT CGAGAGCAAA AAAGCCATCT 
CAATTTTCTG GGAAAATAAC TGTTAAAGCA AAGGAAAAGA GTTATTCTAA ACTCGAAATC 
CCCTACCAAG CAGAAGTTCT AGATGGTTAT TTGGGGTTTG ATCACGCCGC CACGCTGTTT 
CACATCCAGG ACAGTCCTGC TGACCCAGTG GAGAGGCCGA TCTACCTGAC GAACACTTTC 
AGTTTTGCGA TCCTCATTCA TGATGTGCTG TTACCAGAAG AAGCCAGAAT AATGTTTCAG 
GTGCACAACT TCAGCCAGCC GGTTCTCATT CTTCCTAATG AGTCGGGATA CATTTTTACC 
CTGTTTTTTA TGCCATCCAC CTCATCCATG CACATAGACA ACAACATTTT ACTTGTTACC 
AATGCTTCTA AGTTTCATTT ACCTGTGCGG GTATACACAG GCTTTTTAGA TTACTTTGTA 
TTGCCCCCCA AAATAGAAGA ACGCTTCATA GATTTTGGAG TACTGAGTGC TACAGAAGCA 
AGTAGTATTT TATTTGCAAT TATAAACAGC AATCCAATTG AGCTGGCAAT AAAAAGTTGG 
CATATCATAG GAGATGGCTT ATCAATAGAA CTTGTAGCTA CAGAGAGAGG CAACAGAAGC 
ACGGTGATTG CAAGCCTTCC AGAACTGGAG CGGTCCTCTT TGCCCGACCA GTCCCCGGTA 
ACATTAGCTT CAGGACACTT TGCAGTGTTC AGAGTCAAAC TTACTGCCAA GAAGCTAGAA 
GGGGTCCATG ATGGGGCCAT ACAGATCACA ACCGACTACG AGATCCTGAC CATCCCTGTG 
AAAGCTGTGA TCGCCGTGGG CTCGCTGACC TGCTTCCCCA AGCACATGGT TCTTCCCCCT 
TCCTTCCCAG GGAAAATAGT TCATCAGAGC TTAAATATTA TGAATTCCTT CTCACAGAAG 
GTGAAGATAC AGCAAATAAG GTCTTTGTCA GAAGACGTAC GATTCTACTA CAAACGATTA 
CGGGGTAATA GGGAAGACTT GGAGCCAGGG AAGAAGTCTA AGATTGCAAA CATTTATTTT 
GACCCTGGGC TGCAGTGTGG GGATCACTGC TATATCGGCT TGCCTTTCCT ATCCAAATCT 
GAACCCAAAG TACAGCCTGG GGTGGCCATG CAGGAGGACT TGTGGGATGC CGACTGGGAC 
GCACATCAGA GCTTGTTCAA GGCTTGGATG GGGATAAAGG AAAACGCAGG ACATAGGTTG 
AATGCTATGT TTGAAGTAAA TACAGATCTT CAGAAAAATA TCGTATCAAA AGTCAGTGCC 
GAGCTCTCCT GGCCGTCTGT CCTTAGCTCG CCCCGGCTCC TGAAGTTCCC ACTCACTAAC 
ACAAACTGTT CTTCGGAAGA AGAGATTAGC TTAGAAAACC CTGCAGACGT CCCTGTCTAC 
GTCCAGTTCA TCCCACTGGC TTTGTATTCC AACCCCTCCG TCTTTGCAGA TAAGTTAGTC 
TCAAGGTTTA ACTTGAGTAA GGTGGCCAAG CTAGATTTGA GAACACTTGA ATTCCAAGTC 
TACAGGAACA GCGCTCATCC ACTGCAAAGT CCAACAGGAT TTACAGAGGG TCTCTCTCGG 
CATTTCATTT TAAACCTCAT TTTAAAACCT GGAGAAAAGA AATCTGTCAA AGTAAAGTTC 
ACTCCACTCC ACAACAGAAC TGTGTCTTCC CTGATCATAG TCAGAAACAA CCTGACTGTG 
ATGGACGCTG TGATGGTTCA AGGTCAAGGG ACAACGGAGA ACTTGAGGGT GGCAGGCAAG 
CTTCCAGGTC CAGGAAGCTC CTTACGCTTC AAAATCACAG AAGCATTGTT AAAGGATTGC 
ATAGACCGTT TGAAACTAAG AGAACCAAAT TTCACATTGA AAAGAACATT CAAAGTAGAG 
AATACGGGAC AACTTGAAAT CCGTGTAGAA ACCATTGAGA TCAGTGGATA TGCGTGTGAG 
GGATATGGTT TTAAAGTGGT TAACTGTCAA GAGTTTGCCC TGAGTGCCAA CGCCTCCAGA 
GACATAGTCA TATTGTTTAC TCCAGATTTC ACAGCCTCCA GAGTCATTCG GGAGCTGAAG 
TTTGTGACAA GCAGTGGCTC CGAGTTTGTG TTTGTGTTGA ATGCCTCTCT TCCGTACCAC 
ATGCTAGCCG CCTGTGCAGA AGCCCTCCCT AGACCCAACT GGGAGCTCGC GCTCTACATC 
ATCATCTCCG GGGTCATGAG TGCACTCTTT CTCCTGGTCA TTGGAACAGC CTACTTGGAA 
GCTCAAGGGA TTTGGGAGCC CTTCCGAAGG CGACTCTCCT TTGAAGCCTC AAACCCGCCC 
TTTGATGTAG GAAGGCCATT TGATCTCAGG AGAATCGTTG GTATTTCATC TGAAGGAAAC 
TTGAACACAC TTGGCTGCGA ACACAGTCAT GGTAGGGGTT TCTATAGTAA CGCATCATCC 
CGGCCTGGTA CAGGCAGTCA CAGGCAGTGT GGCACATCAG TCCACCCACA CAGCAGCCAT 
GGCAGCAAAA ACTCAGCTGA TGTGGACAAC GTCAGAACCA GAAACAGTTC AAGTATGTCA 
AGCAGGACTT CTCCACAAGC AGCTGCCTCA CAGTCTACAA GCAAAACCAG CCCCCTAGTC 
TCAGAGACAG CTGCAGCAAC CCAGGGTCAC ACAGCAAGCA GGAAGTCCAG AGGGGCAAAG 
CAGGGCCAGC ACAGCAGCCA GCACCACAGC CACAGCCACA GCCCACTGGA GCAGCACTCA 
CAGCCTCCAC CACCAGTGCC TCAGCACCAG GAGCCACCAC CCGAAAGGCT GTCTCCTGCC 
CCCCTCACAC ACCCTTCCCA CCCCGAGCGA GCCAGCACCA CACGGCACAG CTCAGAGGAC 
TCCGACATCA CCAGTCTCAT AGAAGCCATG GACAAAGACT TTGACCACCA CGACTCCTCC 
CCCCTAGACG TGTTTACAGA GCAGCCTCCG TCACCGATGT CAAAAAGCAA AGGGAAAGGA 
AAGTCTCTCC AACAACGTAA GGCCAAGCCT CCGAAGAAGC AGGAGGAGAA GGAGAAGAGG 
GGGAAGGGGA AGCCGCAGGA GGATGAGCTG AAGGACGCTC TGGCCGACGA CGACAGCTCC 
TCCACCACCA CAGAGACCTC CAACCCCGAC ACAGAGCCAC TCCTCAGGGA GGATACAGAG 
AAGCACAAGG GGAGACCAGC CGTGCCTGAA AAACAGGAAA GTGAACTGTC TCAAGGGAAG 
CCCAAGAGCA AAAAGCTCTT AAACGCTAAG AAAGAAATCC CAACAGATGT GAAAGGCAGT 
TCCTTTGAAC TACCGTACAC TCCCTCACTG GAAAATAAAC AGCGTAGAAA TCTCCCAACC 
AAGATTCCTC TTCCAACTAC ACTAGCAAGT GGATCCAAAT CACGAAATCC CCCGAAAACA 
AAAGGTACAA ATAAGTTAGT GGAAAACAGG CCAGTTGCCC TCTCAAAGTT CCTCCCCAGC 
AGTCAAGAAC TAGGTAATAC CAGCAGCTCA GAGGGTGAAA AGGATTCTCC TCCACCAGAA 
TGGGATGCTG TGCCAGTTCA CAAGCCCAGC AGCTACATTT TCTTAAAACA ACGCCAGACC 
TCGCCAACAC CTGCCTCTCC TTCTCTGCCC ACCGCTCCCT GCCCGTTCAC ATCTCGTGGC 
AGCTACAGCA GCGTTGTCAA CAGCTCCGGC AGTGACACAA AAGCCAAGCA GACGAGCAGC 
AGCAAGAGCA AGCTGACAAA GGCGGCCTCG CTCCCCGGCA AGAATGGCAA CCCTACCTTC 
GCCGCCGTCG CTGCAGGCTA TGACAAGAGC CCGGGTGGGA ATGGATTTGC TAAAATTTCT 
TCAAACAAAT CAGATTTTTC CAGCAGCCTT GGCATTTCAC ACATTCCTGT TGACAGCGAT 
GGCTCTGACA GCTCGGGTTT GTGGAGTCCT GTCAGCAACC CCAACAGCCC TGACTTCACC 
CCCCTCAATT CGTTCTCAGC CTTTGGAAAT TCTTTTAATC TAACAGGTGC AGTTTTCAGC 
AAACTGTCGC GATCCTGCAG TCAGTCGTCC CAGAGAAGTT GGAATGAGTT CAACAGCGGC 
CCCTCCTACC TTTGGGATTC TCCAGCCACA GACCCCAGCC CTTCCTGGCC GGCCAGTTCC 
AGCTCCCCAA CCCACACAGC CACTTCCATC CTTGGCAACA GCAGCGGCTT GTGGTCCACC 
ACTCCATTCA GCAGCTCCAT CTGGTCCAGC AACATCAACA GCAACCTTCC TTTCTCCACT 
CCAACAAACG CGCTGTCGAG CATCAGCCTC ATGGGCACGG AAAACTCTGC CGCCGCCCAC 
ACCCCTTCCG CCTCTGGCCC AGCTGACGAC TTGGGACAGA CCTACAACCC CTGGCGGATC 
TGGAGCCCCA CAGTCGGAAG AAGAAGCTCT GACCCTTGGT CTAATTCGCA CTTCCCTCAT 
GAAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177303.1
CDS	510..6098
Translation	MGKRAGGAAAAAAAASTSSAAGLEPAAGRGGGPRSAAAGLLGALHLVMTLVVAAARAEKEAFIQSESIIEVLRFDDGGLLQTETTLGLGSYQQKSISLYRGNCRPIRFEPPMLDFHEQPVGMPKMEKVYLHNPSSEETITLVSISATTSHFHASFFQNRKILPGGNTSFDVVFLARVVGNVENTLFINTSNHGVFTYQVFGVGVPNPYRLRPFLGARVPVNSSFSPIINIHNPHSEPLQVVEMYSSGGDLHLELPTGQQGGTRKLWEIPPYETKGVMRASFSSREADNHTAFIRIKTNASDSTEFIILPVEVEVTTAPGIYSSTEMLDFGTLRTQDLPKVLNLHLLNSGTKDVPITSVRPTPQNDAITVHFKPVTLKASESKYTKVASISFDASRAKKPSQFSGKITVKAKEKSYSKLEIPYQAEVLDGYLGFDHAATLFHIQDSPADPVERPIYLTNTFSFAILIHDVLLPEEARIMFQVHNFSQPVLILPNESGYIFTLFFMPSTSSMHIDNNILLVTNASKFHLPVRVYTGFLDYFVLPPKIEERFIDFGVLSATEASSILFAIINSNPIELAIKSWHIIGDGLSIELVATERGNRSTVIASLPELERSSLPDQSPVTLASGHFAVFRVKLTAKKLEGVHDGAIQITTDYEILTIPVKAVIAVGSLTCFPKHMVLPPSFPGKIVHQSLNIMNSFSQKVKIQQIRSLSEDVRFYYKRLRGNREDLEPGKKSKIANIYFDPGLQCGDHCYIGLPFLSKSEPKVQPGVAMQEDLWDADWDAHQSLFKAWMGIKENAGHRLNAMFEVNTDLQKNIVSKVSAELSWPSVLSSPRLLKFPLTNTNCSSEEEISLENPADVPVYVQFIPLALYSNPSVFADKLVSRFNLSKVAKLDLRTLEFQVYRNSAHPLQSPTGFTEGLSRHFILNLILKPGEKKSVKVKFTPLHNRTVSSLIIVRNNLTVMDAVMVQGQGTTENLRVAGKLPGPGSSLRFKITEALLKDCIDRLKLREPNFTLKRTFKVENTGQLEIRVETIEISGYACEGYGFKVVNCQEFALSANASRDIVILFTPDFTASRVIRELKFVTSSGSEFVFVLNASLPYHMLAACAEALPRPNWELALYIIISGVMSALFLLVIGTAYLEAQGIWEPFRRRLSFEASNPPFDVGRPFDLRRIVGISSEGNLNTLGCEHSHGRGFYSNASSRPGTGSHRQCGTSVHPHSSHGSKNSADVDNVRTRNSSSMSSRTSPQAAASQSTSKTSPLVSETAAATQGHTASRKSRGAKQGQHSSQHHSHSHSPLEQHSQPPPPVPQHQEPPPERLSPAPLTHPSHPERASTTRHSSEDSDITSLIEAMDKDFDHHDSSPLDVFTEQPPSPMSKSKGKGKSLQQRKAKPPKKQEEKEKRGKGKPQEDELKDALADDDSSSTTTETSNPDTEPLLREDTEKHKGRPAVPEKQESELSQGKPKSKKLLNAKKEIPTDVKGSSFELPYTPSLENKQRRNLPTKIPLPTTLASGSKSRNPPKTKGTNKLVENRPVALSKFLPSSQELGNTSSSEGEKDSPPPEWDAVPVHKPSSYIFLKQRQTSPTPASPSLPTAPCPFTSRGSYSSVVNSSGSDTKAKQTSSSKSKLTKAASLPGKNGNPTFAAVAAGYDKSPGGNGFAKISSNKSDFSSSLGISHIPVDSDGSDSSGLWSPVSNPNSPDFTPLNSFSAFGNSFNLTGAVFSKLSRSCSQSSQRSWNEFNSGPSYLWDSPATDPSPSWPASSSSPTHTATSILGNSSGLWSTTPFSSSIWSSNINSNLPFSTPTNALSSISLMGTENSAAAHTPSASGPADDLGQTYNPWRIWSPTVGRRSSDPWSNSHFPHEN

Target ORF information:

RefSeq Version	XM_017321814.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane protein 131 (Tmem131), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321814.1

ORF Insert Sequence:

ATGGGGAAGC GGGCGGGAGG AGCAGCCGCC GCCGCCGCCG CCGCCTCCAC GTCCTCCGCG 
GCCGGGCTGG AGCCCGCGGC TGGCCGAGGC GGGGGTCCTC GGAGCGCAGC CGCGGGCCTC 
CTGGGAGCGC TGCACCTGGT GATGACCCTC GTGGTGGCCG CGGCGCGGGC CGAGAAGGAA 
GCATTCATTC AGTCGGAGAG CATAATAGAA GTCCTGCGCT TTGACGACGG AGGGCTACTA 
CAGACTGAGA CAACACTTGG CCTCGGTTCA TATCAACAGA AAAGCATTTC TCTGTACCGG 
GGTAATTGCA GGCCCATACG ATTCGAACCG CCCATGCTGG ATTTCCATGA GCAGCCAGTT 
GGAATGCCAA AAATGGAAAA AGTCTACTTA CATAACCCTA GTTCTGAAGA AACGATTACT 
TTAGTATCAA TATCTGCTAC CACATCACAT TTCCATGCAT CGTTTTTTCA AAATAGGAAA 
ATTCTTCCAG GAGGAAACAC ATCATTTGAT GTAGTTTTTC TTGCAAGAGT AGTAGGAAAT 
GTAGAAAATA CTTTATTTAT TAACACATCT AACCATGGGG TGTTCACTTA CCAGGTATTT 
GGTGTTGGGG TTCCAAATCC GTATCGATTA AGGCCATTTC TTGGGGCCAG AGTCCCCGTA 
AACAGCAGCT TTTCACCCAT AATAAACATA CACAATCCTC ACAGTGAGCC TTTACAGGTC 
GTAGAGATGT ACTCGAGTGG AGGTGACCTT CACCTAGAGC TCCCAACAGG TCAGCAAGGA 
GGCACCAGGA AGCTGTGGGA AATCCCTCCT TATGAAACCA AGGGAGTGAT GAGAGCCAGC 
TTTTCCTCCA GAGAAGCAGA CAATCACACA GCCTTCATCC GGATAAAGAC GAATGCATCA 
GACAGCACCG AGTTCATCAT TCTCCCGGTT GAGGTTGAAG TTACAACAGC TCCTGGAATC 
TATTCCTCAA CTGAAATGTT AGATTTTGGA ACACTTAGAA CACAAGATCT ACCAAAGGTT 
TTAAATCTTC ATTTACTAAA TTCAGGAACA AAGGATGTAC CAATAACAAG TGTTCGACCA 
ACACCACAAA ATGACGCTAT CACGGTACAT TTTAAACCAG TTACGTTAAA AGCTTCTGAA 
AGTAAATATA CCAAGGTTGC CAGCATTAGT TTTGATGCTT CGAGAGCAAA AAAGCCATCT 
CAATTTTCTG GGAAAATAAC TGTTAAAGCA AAGGAAAAGA GTTATTCTAA ACTCGAAATC 
CCCTACCAAG CAGAAGTTCT AGATGGTTAT TTGGGGTTTG ATCACGCCGC CACGCTGTTT 
CACATCCAGG ACAGTCCTGC TGACCCAGTG GAGAGGCCGA TCTACCTGAC GAACACTTTC 
AGTTTTGCGA TCCTCATTCA TGATGTGCTG TTACCAGAAG AAGCCAGAAT AATGTTTCAG 
GTGCACAACT TCAGCCAGCC GGTTCTCATT CTTCCTAATG AGTCGGGATA CATTTTTACC 
CTGTTTTTTA TGCCATCCAC CTCATCCATG CACATAGACA ACAACATTTT ACTTGTTACC 
AATGCTTCTA AGTTTCATTT ACCTGTGCGG GTATACACAG GCTTTTTAGA TTACTTTGTA 
TTGCCCCCCA AAATAGAAGA ACGCTTCATA GATTTTGGAG TACTGAGTGC TACAGAAGCA 
AGTAGTATTT TATTTGCAAT TATAAACAGC AATCCAATTG AGCTGGCAAT AAAAAGTTGG 
CATATCATAG GAGATGGCTT ATCAATAGAA CTTGTAGCTA CAGAGAGAGG CAACAGAAGC 
ACGGTGATTG CAAGCCTTCC AGAACTGGAG CGGTCCTCTT TGCCCGACCA GTCCCCGGTA 
ACATTAGCTT CAGGACACTT TGCAGTGTTC AGAGTCAAAC TTACTGCCAA GAAGCTAGAA 
GGGGTCCATG ATGGGGCCAT ACAGATCACA ACCGACTACG AGATCCTGAC CATCCCTGTG 
AAAGCTGTGA TCGCCGTGGG CTCGCTGACC TGCTTCCCCA AGCACATGGT TCTTCCCCCT 
TCCTTCCCAG GGAAAATAGT TCATCAGAGC TTAAATATTA TGAATTCCTT CTCACAGAAG 
GTGAAGATAC AGCAAATAAG GTCTTTGTCA GAAGACGTAC GATTCTACTA CAAACGATTA 
CGGGGTAATA GGGAAGACTT GGAGCCAGGG AAGAAGTCTA AGATTGCAAA CATTTATTTT 
GACCCTGGGC TGCAGTGTGG GGATCACTGC TATATCGGCT TGCCTTTCCT ATCCAAATCT 
GAACCCAAAG TACAGCCTGG GGTGGCCATG CAGGAGGACT TGTGGGATGC CGACTGGGAC 
GCACATCAGA GCTTGTTCAA GGCTTGGATG GGGATAAAGG AAAACGCAGG ACATAGGTTG 
AATGCTATGT TTGAAGTAAA TACAGATCTT CAGAAAAATA TCGTATCAAA AGTCAGTGCC 
GAGCTCTCCT GGCCGTCTGT CCTTAGCTCG CCCCGGCTCC TGAAGTTCCC ACTCACTAAC 
ACAAACTGTT CTTCGGAAGA AGAGATTAGC TTAGAAAACC CTGCAGACGT CCCTGTCTAC 
GTCCAGTTCA TCCCACTGGC TTTGTATTCC AACCCCTCCG TCTTTGCAGA TAAGTTAGTC 
TCAAGGTTTA ACTTGAGTAA GGTGGCCAAG CTAGATTTGA GAACACTTGA ATTCCAAGTC 
TACAGGAACA GCGCTCATCC ACTGCAAAGT CCAACAGGAT TTACAGAGGG TCTCTCTCGG 
CATTTCATTT TAAACCTCAT TTTAAAACCT GGAGAAAAGA AATCTGTCAA AGTAAAGTTC 
ACTCCACTCC ACAACAGAAC TGTGTCTTCC CTGATCATAG TCAGAAACAA CCTGACTGTG 
ATGGACGCTG TGATGGTTCA AGGTCAAGGG ACAACGGAGA ACTTGAGGGT GGCAGGCAAG 
CTTCCAGGTC CAGGAAGCTC CTTACGCTTC AAAATCACAG AAGCATTGTT AAAGGATTGC 
ATAGACCGTT TGAAACTAAG AGAACCAAAT TTCACATTGA AAAGAACATT CAAAGTAGAG 
AATACGGGAC AACTTGAAAT CCGTGTAGAA ACCATTGAGA TCAGTGGATA TGCGTGTGAG 
GGATATGGTT TTAAAGTGGT TAACTGTCAA GAGTTTGCCC TGAGTGCCAA CGCCTCCAGA 
GACATAGTCA TATTGTTTAC TCCAGATTTC ACAGCCTCCA GAGTCATTCG GGAGCTGAAG 
TTTGTGACAA GCAGTGGCTC CGAGTTTGTG TTTGTGTTGA ATGCCTCTCT TCCGTACCAC 
ATGCTAGCCG CCTGTGCAGA AGCCCTCCCT AGACCCAACT GGGAGCTCGC GCTCTACATC 
ATCATCTCCG GGGTCATGAG TGCACTCTTT CTCCTGGTCA TTGGAACAGC CTACTTGGAA 
GCTCAAGGGA TTTGGGAGCC CTTCCGAAGG CGACTCTCCT TTGAAGCCTC AAACCCGCCC 
TTTGATGTAG GAAGGCCATT TGATCTCAGG AGAATCGTTG GTATTTCATC TGAAGGAAAC 
TTGAACACAC TTGGCTGCGA ACACAGTCAT GGTAGGGGTT TCTATAGTAA CGCATCATCC 
CGGCCTGGTA CAGGCAGTCA CAGGCAGTGT GGCACATCAG TCCACCCACA CAGCAGCCAT 
GGCAGCAAAA ACTCAGCTGA TGTGGACAAC GTCAGAACCA GAAACAGTTC AAGTATGTCA 
AGCAGGACTT CTCCACAAGC AGCTGCCTCA CAGTCTACAA GCAAAACCAG CCCCCTAGTC 
TCAGAGACAG CTGCAGCAAC CCAGGGTCAC ACAGCAAGCA GGAAGTCCAG AGGGGCAAAG 
CAGGGCCAGC ACAGCAGCCA GCACCACAGC CACAGCCACA GCCCACTGGA GCAGCACTCA 
CAGCCTCCAC CACCAGTGCC TCAGCACCAG GAGCCACCAC CCGAAAGGCT GTCTCCTGCC 
CCCCTCACAC ACCCTTCCCA CCCCGAGCGA GCCAGCACCA CACGGCACAG CTCAGAGGAC 
TCCGACATCA CCAGTCTCAT AGAAGCCATG GACAAAGACT TTGACCACCA CGACTCCTCC 
CCCCTAGACG TGTTTACAGA GCAGCCTCCG TCACCGATGT CAAAAAGCAA AGGGAAAGGA 
AAGTCTCTCC AACAACGTAA GGCCAAGCCT CCGAAGAAGC AGGAGGAGAA GGAGAAGAGG 
GGGAAGGGGA AGCCGCAGGA GGATGAGCTG AAGGACGCTC TGGCCGACGA CGACAGCTCC 
TCCACCACCA CAGAGACCTC CAACCCCGAC ACAGAGCCAC TCCTCAGGGA GGATACAGAG 
AAGCACAAGG GGAGACCAGC CGTGCCTGAA AAACAGGAAA GTGAACTGTC TCAAGGGAAG 
CCCAAGAGCA AAAAGCTCTT AAACGCTAAG AAAGAAATCC CAACAGATGT GAAAGGCAGT 
TCCTTTGAAC TACCGTACAC TCCCTCACTG GAAAATAAAC AGCGTAGAAA TCTCCCAACC 
AAGATTCCTC TTCCAACTAC ACTAGCAAGT GGATCCAAAT CACGAAATCC CCCGAAAACA 
AAAGGTACAA ATAAGTTAGT GGAAAACAGG CCAGTTGCCC TCTCAAAGTT CCTCCCCAGC 
AGTCAAGAAC TAGGTAATAC CAGCAGCTCA GAGGGTGAAA AGGATTCTCC TCCACCAGAA 
TGGGATGCTG TGCCAGTTCA CAAGCCCAGC AGCTACATTT TCTTAAAACA ACGCCAGACC 
TCGCCAACAC CTGCCTCTCC TTCTCTGCCC ACCGCTCCCT GCCCGTTCAC ATCTCGTGGC 
AGCTACAGCA GCGTTGTCAA CAGCTCCGGC AGTGACACAA AAGCCAAGCA GACGAGCAGC 
AGCAAGAGCA AGCTGACAAA GGCGGCCTCG CTCCCCGGCA AGAATGGCAA CCCTACCTTC 
GCCGCCGTCG CTGCAGGCTA TGACAAGAGC CCGGGTGGGA ATGGATTTGC TAAAATTTCT 
TCAAACAAAT CAGATTTTTC CAGCAGCCTT GGCATTTCAC ACATTCCTGT TGACAGCGAT 
GGCTCTGACA GCTCGGGTTT GTGGAGTCCT GTCAGCAACC CCAACAGCCC TGACTTCACC 
CCCCTCAATT CGTTCTCAGC CTTTGGAAAT TCTTTTAATC TAACAGGTGC AGTTTTCAGC 
AAACTGTCGC GATCCTGCAG TCAGTCGTCC CAGAGAAGTT GGAATGAGTT CAACAGCGGC 
CCCTCCTACC TTTGGGATTC TCCAGCCACA GACCCCAGCC CTTCCTGGCC GGCCAGTTCC 
AGCTCCCCAA CCCACACAGC CACTTCCATC CTTGGCAACA GCAGCGGCTT GTGGTCCACC 
ACTCCATTCA GCAGCTCCAT CTGGTCCAGC AACATCAACA GCAACCTTCC TTTCTCCACT 
CCAACAAACG CGCTGTCGAG CATCAGCCTC ATGGGCACGG AAAACTCTGC CGCCGCCCAC 
ACCCCTTCCG CCTCTGGCCC AGCTGACGAC TTGGGACAGA CCTACAACCC CTGGCGGATC 
TGGAGCCCCA CAGTCGGAAG AAGAAGCTCT GACCCTTGGT CTAATTCGCA CTTCCCTCAT 
GAAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tagging genes with cassette-exchange sites.
Nucleic acids research33(4)e44(2005 Mar)
Cobellis G,Nicolaus G,Iovino M,Romito A,Marra E,Barbarisi M,Sardiello M,Di Giorgio FP,Iovino N,Zollo M,Ballabio A,Cortese R

Neg, a nerve growth factor-stimulated gene expressed by fetal neocortical neurons that is downregulated by ethanol.
The Journal of comparative neurology460(2)212-22(2003 May)
Miller MW,Jacobs JS,Yokoyama R

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes9(5)179-88(2002 Oct)
Okazaki N,Kikuno R,Ohara R,Inamoto S,Hara Y,Nagase T,Ohara O,Koga H

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Genome research11(9)1553-8(2001 Sep)
Piao Y,Ko NT,Lim MK,Ko MS

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Tmem131 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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