TMEM131 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TMEM131
Entrez Gene ID	540861
Full Name	transmembrane protein 131
General protein information	Preferred Names transmembrane protein 131 Names transmembrane protein 131
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002691141.5	XP_002691187.1	transmembrane protein 131 isoform X1
XM_015465285.1	XP_015320771.1	transmembrane protein 131 isoform X3
XM_002691141.6	XP_002691187.1	transmembrane protein 131 isoform X1
XM_613474.9	XP_613474.6	transmembrane protein 131 isoform X1
XM_015473357.1	XP_015328843.1	transmembrane protein 131 isoform X3
XM_005193810.3	XP_005193867.1	transmembrane protein 131 isoform X2
XM_005212403.3	XP_005212460.1	transmembrane protein 131 isoform X2
XM_005212403.4	XP_005212460.1	transmembrane protein 131 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TMEM131	XP_515638.2
	TMEM131	XP_002808093.1
Danio rerio (zebrafish)	tmem131	XP_005166018.1
Xenopus tropicalis (tropical clawed frog)	tmem131	NP_001135552.1
Bos taurus (cattle)	TMEM131	XP_002691187.1
Homo sapiens (human)	TMEM131	NP_056163.1
Canis lupus familiaris (dog)	TMEM131	XP_531794.3
Rattus norvegicus (Norway rat)	Tmem131	XP_237056.4
Gallus gallus (chicken)	TMEM131	NP_001012712.2
Mus musculus (house mouse)	Tmem131	NP_061360.2

Gene Ontology

The following TMEM131 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEM131 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa91812	XM_015465285.1 Latest version!	Bos taurus transmembrane protein 131 (TMEM131), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa91812	XM_015473357.1 Latest version!	Bos taurus transmembrane protein 131 (TMEM131), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa06771	XM_002691141.6 Latest version!	Bos taurus transmembrane protein 131 (TMEM131), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa06771	XM_613474.9 Latest version!	Bos taurus transmembrane protein 131 (TMEM131), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa06772	XM_005193810.3 Latest version!	Bos taurus transmembrane protein 131 (TMEM131), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa06772	XM_005212403.4 Latest version!	Bos taurus transmembrane protein 131 (TMEM131), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
OBa06771	XM_002691141.5	Bos taurus transmembrane protein 131 (TMEM131), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa06772	XM_005212403.3	Bos taurus transmembrane protein 131 (TMEM131), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMEM131 ( XM_015465285.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320771.1 Bos taurus transmembrane protein 131 (TMEM131), transcript variant X3, mRNA.

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CloneID	OBa91812
Clone ID Related Accession (Same CDS sequence)	XM_015465285.1 , XM_015473357.1
Accession Version	XM_015465285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transmembrane protein 131 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645888.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGACA TTTCAGATTT TACTTTGTTG GGTGCGGTGT TTTGGATTCC AAGGAGTATT 
CTTGAAAGAG CTTTGTTTTC TGATGCAGCA TTTATTCAGT CGGAGAGCAT AATAGAAGTG 
CTGCGTTTTG ATGATGGAGG GCTTCTACAG ACTGAGACAA CACTTGGACT CAGTTCATAC 
CAACAGAAAA GTATATCTTT GTACCGGGGT AATTGCAGAC CTATACGGTT TGAGCCACCA 
ATGCTGGATT TCCATGAACA ACCAGTTGGA ATGCCAAAAA TGGAAAAAGT TTACTTACAT 
AATCCTAGTT CTGAAGAAAC TATTACTTTA GTATCAATAT CTGCTACCAC ATCACATTTT 
CACGCATCAT TTTTTCAAAA TAGGAAAATT CTTCCAGGAG GAAATACATC ATTTGATGTA 
GTTTTTCTTG CAAGAGTAGT GGGAAATGTA GAAAATACTT TATTTATTAA TACATCTAAT 
CATGGGGTAT TCACTTACCA GGTATTTGGT GTTGGAGTTC CAAATCCATA CCGATTGAGG 
CCATTCCTTG GGGCCAGAGT CCCTGTGAAT AGCAGTTTCT CACCCATAAT AAACATACAC 
AATCCTCACA GCGAGCCTTT ACAGGTTGTA GAAATGTACT CTAGTGGAGG AGACCTTCAC 
CTTGAACTTC CCACAGGGCA GCAGGGAGGC ACCAGGAAGC TGTGGGAAAT TCCTCCTTAT 
GAAACCAAGG GAGTGATGAG AGCCAGTTTT TCATCTAGAG AAGCAGATAA TCACACAGCT 
TTCATAAGAA TAAAGACAAA TGCTTCAGAC AGCACGGAGT TTATCATCCT TCCGGTTGAA 
GTGGAAGTCA CAACAGCTCC TGGAATTTAT TCCTCAACTG AAATGTTAGA TTTTGGCACA 
CTGCGAACAC AAGATCTACC AAAAGTTTTA AATCTTCATT TATTAAATTC AGGAACAAAA 
GATGTACCAA TAACAAGTGT TCGACCTACA CCACAAAATG ATGCTGTCAC TGTACACTTT 
AAACCAATTA CATTAAAAGC TTCAGAAAGT AAATATACCA AGGTAGCAAG TATTAGTTTT 
GATGCATCAA AGGCGAAAAA ACCATCTCAG TTTTCTGGGA AAATAACTGT GAAAGCAAAG 
GAAAAGAGTT ATTCTAAACT TGAAATACCA TATCAAGCAG AGGTTTTAGA TGGTTATTTG 
GGATTTGATC ACGCTGCAAC ATTATTTCAC ATTCGAGACA GTCCTGCTGA TCCTGTGGAA 
AGGCCAATTT ACCTTACTAA CACTTTCAGC TTTGCGATCC TCATTCATGA TGTGTTGCTA 
CCAGAAGAAG CCAAAACAAT GTTTAAAGTT CACAACTTCA GCAAACCAGT CTTAATTCTA 
CCAAATGGAT CAGGCTACAT TTTTACTCTA TTTTTTATGC CATCCACATC GTCCATGCAC 
ATAGATAACA ATATTTTACT TATTACCAAT GCCTCTAAGT TTCACTTACC TGTGCGGGTA 
TATACAGGCT TTTTAGATTA CTTTGTATTA CCCCCCAAAA TAGAAGAACG TTTCATAGAT 
TTTGGTATAC TGAGTGCAAC AGAAGCAAGT AATATTTTAT TTGCCATTAT AAACAGCAAT 
CCAATTGAGT TGGCTATAAA AAGCTGGCAT ATTATAGGAG ATGGTTTATC AATAGAACTC 
GTAGCTACTG AAAGAGGCAA TAGAACTACA ATCATCTCAA GCCTTCCAGA GTTTGAAAAA 
TCCTCTCTAT CAGACCAGTC ATCGGTAATA CTAGCTTCAG GATACTTTGC AGTGTTCAGA 
GTCAAACTTA CTGCAAAAAA ACTGGAAGGA ATTCATGATG GGGCTATACA GATCACGACA 
GACTATGAGA TCCTAACAAT TCCTGTGAAG GCTGTGATCG CAGTGGGTTC TTTGACTTGC 
TTCCCAAAGC ACATGGTTCT TCCACCTTCC TTTCCAGGGA AAATAGTTCA TCAGAGTTTA 
AATATTATGA ATTCCTTCTC ACAGAAGGTA AAGATCCAAC AGATACGATC TTTGTCAGAA 
GACGTGCGTT TTTACTACAA ACGATTGCGG GGTAATAAGG AAGACTTGGA GCCAGGGAAA 
AAATCAAAGA TTGCAAACAT TTACTTTGAT CCTGGATTGC AGTGCGGGGA TCATTGCTAT 
GTCGGCTTGC CTTTTCTGTC CAAATCTGAA CCCAGAGTCC AGCCTGGCAT AGCCATGCAG 
GAAGATGCCT GGGACACCGA CTGGGATTTG CATCAAAGCC TATTCAAGGG ATGGATGGGA 
ATAAAGGAAA ACTCAGGTCA CAAATTGAGC GCTATGTTTG AAGTGAATAC AGACCTTCAG 
AAAAACATCC TGTCAAAAGT TTCTGCCGAG CTCTCCTGGC CATCCCTCCT CAGCTCCCCC 
CGGCTCGTGA GGTTTCCCCT CACTAATACA AACTGCTCTT CAGAAGAAGA GATTATGTTA 
GAAAACCCTG CAGATGTTCC CATCTATATT CAATTTATCC CCCTGGCTTT ATATTCCAGC 
CCTTCCATCT TTGTAGATAA GTTAATATCA AGGTTTAACT TAAGTAAGGT GGCAAAGATC 
GATTTAAGAA CACAAGAATT CCAAGTCTTT AGAAACACTG CTCACCCACT GCAGAGTTCA 
ACAGGATTCA CAGAGGGCCT CTCCCGACAT TTAGTTTTAA ACTTAATTTT AAAACCTGGA 
GAAAAGAAAT CTGTCAAAGT AAAGTTTACT CCAGTTCACA ATAGAACTGT TTCTTCCCTA 
ATCATAATCA GAAATAACCT GACTGTGATG GATGCCGTAA TGGTCCAAGG ACAGGGGACA 
ACCGAGAACC TGAGGGTGGC AGGCAAGCTT CCGGGCCCAG GGAGCTCCTT ACGCTTCAAA 
ATCACAGAAG CACTGCTGAA AGACTGTACG GAGGGTATGA GACTAAGGGA ACCCAATTTC 
ACATTGAAAA GAACATTTAA AGTAGAGAAC ACAGGACAAC TTCCAATTCA CATAGAAACC 
ATTGAAGTCA GTGGGTACTC TTGTGAGGGA TATGGCTTTA AAGTCGTCAA TTGTCAAGAG 
TTTGCACTGA GCGCCAATGC CTCTAAAGAC ATAGTCATTC TGTTTACTCC TGATTTCACG 
GCTTCTAGAG TCATCCGTGA ACTGAAGTTT ATAACAGCCA GTGGCTCTGA GTTTGTGTTT 
GTATTGAATG CATCCCTTCC TTACCATATG TTAGCAACCT GTGCAGAAGC CCTGCCCAGG 
CCCAACTGGG AGCTGGCTCT GTACATCATC ATCTCAGGAA TAATGAGTGC GCTGTTTCTC 
TTGGTAATTG GAACAGCCTA TTTAGAAGCT CAAGGAATTT GGGAGCCCTT CCGAAGGCGG 
CTGTCCTTTG AGGCCTCGAA CCCGCCCTTT GATGTGGGGA GACCATTTGA TCTCAGGAGA 
ATCGTTGGTA TTTCATCTGA AGGAAACTTG AACACACTCA GCTGTGACCC CGGTCACAGT 
CGGGGCTTCT GTGGAGCAGG TGGCTCGGCC TCCCGGCCCG GCGCAGGGAG TCACAAGCAG 
TGTAGCCCGT CGGTCCACCC GCACAGCAGT CACAGCAATA GAAACTCGGC TGACGTGGAA 
AACGTCAGAG CCAAAAACAG TGCGAGTGCC TCTGGCAGGA CTAATGCCCA GTCGGCGAGC 
AAAGCCAGCC CACTGGTCTT CGAGTCCCAC AGCATGGCCC AGGGTCACAC CGCAGGCCGG 
AAGTCCAAGG GGGCCAGACA GGGCCAGCAC AGCAGCCAGC ACCATGGCCA CAGCCCCCTG 
GAACAGCACT CGCCCCCGCC GCCGCCGCCA GTGCCTCAGC AGCAGGAGCC ACAGCCCGAG 
AGGCTGTCCC CTGCGCCCCT CGCACCCCCG CCCCACCCAG AGCACCCCGG CAGCACCCGG 
CACAGCTCCG AGGACTCAGA CATCACCAGT CTCATCGAAG CCATGGACAA AGACTTCGAG 
CACCACGACG CCCCGCCCCT AGATGTGTTT ACAGAGCAGC CGCCTTCACC CTTGTCGAAA 
AGCAAAGGGA AAGGAAAGCC TCCTCAGCGC AAGGTGAAGC CCCCCAAGAA GCAGGAGGAG 
AAGGAGAAGA AGGGAAAGGG GAGGGCCCTG GAAGACGAGC TGAAGGACTC CTTGGCTGAC 
GACGACAGCT CCTCCACCAC CACAGAGACC TCCAACCCAG ACACAGAGCC TCTCCTCAAG 
GAGGATACAG AAAAGCAAAA GGGAAAACAA GCCATGCCTG AAAAACATGA AAACGAAATG 
TCTCAAGTGA AACAAAAAAG CAAAAAAGTC TTAAATGTTA AGAAAGAAAT CCCAGCAGAT 
GTGAAACCCA GTTGCTTAGA GCTACCGTAT ACTGCCCCCT TGGAAAGCAA ACAGCGCCGA 
AATCTCCCAA CCAAGATTCC TCTTCCAACT ACACTGACCA GTGGATCCAA ACCACGAAAC 
TCCCAGAAAA CAAAAGGTAC AAGTAAGTTA GTGGATAGCA GGCCACCTGC CCTCGCAAAG 
TTTCTCCCAA ACAGTCAAGA ACTAGGCAAC ACCAGCAGCT CAGAAGGTGA AAAAGACTCT 
CCACCACCAG AGTGGGATTC CGTGCCTGTT CACAAGCCCG GCAGCTCTAC AGATAGTCTT 
TATAAACTCT CTCTGCAAAC CCTCAACGCA GACATCTTCC TGAAACAACG CCAGACCTCG 
CCGCCGCCCA CCTCCCCCTC ACCGCCCGCC GCACCCTGCC CGTTCACGTC CCGGGGCAGC 
TACAGCAGCA TCGTCCATGG CAGCCCGGGC AGCGACTCTA AAGCAAAGCA GCCAAGCGGG 
AGCAAACACA AACTGACAAA AGCCGCTTCT CTCCCGGGCA AGAATGGCAA CCCCACTTTC 
GCTGCGGTCA CGGCTGGGTA TGACAAGAGC CCAGGTGGGA ACGGCTTTGC CAAAGTTTCT 
TCAAGCAAAA CAGATTTCTC TAGCAGCCTT GGCATTTCAC ACACTCCTGT TGACAGCGAT 
GGCTCAGACA GCTCGGGTCT GTGGAGCCCC GTGAGCAACC CCAGCAGCCC TGACTTCACA 
CCCCTCAACT CGTTCTCAGC CTTTGGGAAT TCTTTTAACC TAACTGGTGA AGTGTTCAGC 
AAGCTCGGGT TATCGCGATC ATGCAATCAG CCCTCACAGA GGAGCTGGAA TGAGCTGAGC 
AGCGGCCCCT CCTACCTCTG GGACTCGCCG GCCACAGAGC CCAGCCCTTC CTGGCCAGCC 
AGTTCCAGCT CCCCGACCCA CACGGCCACA CCCATCCTTG GCAGCACCAG CAGCCTGTGG 
TCGTCCACTC CGTTCAGCAG CTCCATCTGG TCCAGCAGCC TGCACAGCAC CCTGCCCTTC 
ACCAGCCCCA CCAGCGCGTT GCCGAGCATC AGCCTCATGG GCCCCGAGAG CGCCCCGGCA 
GCTCACGCGC CCTCTGCCGC CAGCCCCTCC GACGACCTGG GCCAGACCTA CAACCCCTGG 
CGGATATGGA GTCCCACCAT CGGACGGAGG AGCTCAGACC CCTGGTCGAA TTCGCACTTC 
CCTCACGAGA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320771.1
CDS	16..5550
Translation	MPDISDFTLLGAVFWIPRSILERALFSDAAFIQSESIIEVLRFDDGGLLQTETTLGLSSYQQKSISLYRGNCRPIRFEPPMLDFHEQPVGMPKMEKVYLHNPSSEETITLVSISATTSHFHASFFQNRKILPGGNTSFDVVFLARVVGNVENTLFINTSNHGVFTYQVFGVGVPNPYRLRPFLGARVPVNSSFSPIINIHNPHSEPLQVVEMYSSGGDLHLELPTGQQGGTRKLWEIPPYETKGVMRASFSSREADNHTAFIRIKTNASDSTEFIILPVEVEVTTAPGIYSSTEMLDFGTLRTQDLPKVLNLHLLNSGTKDVPITSVRPTPQNDAVTVHFKPITLKASESKYTKVASISFDASKAKKPSQFSGKITVKAKEKSYSKLEIPYQAEVLDGYLGFDHAATLFHIRDSPADPVERPIYLTNTFSFAILIHDVLLPEEAKTMFKVHNFSKPVLILPNGSGYIFTLFFMPSTSSMHIDNNILLITNASKFHLPVRVYTGFLDYFVLPPKIEERFIDFGILSATEASNILFAIINSNPIELAIKSWHIIGDGLSIELVATERGNRTTIISSLPEFEKSSLSDQSSVILASGYFAVFRVKLTAKKLEGIHDGAIQITTDYEILTIPVKAVIAVGSLTCFPKHMVLPPSFPGKIVHQSLNIMNSFSQKVKIQQIRSLSEDVRFYYKRLRGNKEDLEPGKKSKIANIYFDPGLQCGDHCYVGLPFLSKSEPRVQPGIAMQEDAWDTDWDLHQSLFKGWMGIKENSGHKLSAMFEVNTDLQKNILSKVSAELSWPSLLSSPRLVRFPLTNTNCSSEEEIMLENPADVPIYIQFIPLALYSSPSIFVDKLISRFNLSKVAKIDLRTQEFQVFRNTAHPLQSSTGFTEGLSRHLVLNLILKPGEKKSVKVKFTPVHNRTVSSLIIIRNNLTVMDAVMVQGQGTTENLRVAGKLPGPGSSLRFKITEALLKDCTEGMRLREPNFTLKRTFKVENTGQLPIHIETIEVSGYSCEGYGFKVVNCQEFALSANASKDIVILFTPDFTASRVIRELKFITASGSEFVFVLNASLPYHMLATCAEALPRPNWELALYIIISGIMSALFLLVIGTAYLEAQGIWEPFRRRLSFEASNPPFDVGRPFDLRRIVGISSEGNLNTLSCDPGHSRGFCGAGGSASRPGAGSHKQCSPSVHPHSSHSNRNSADVENVRAKNSASASGRTNAQSASKASPLVFESHSMAQGHTAGRKSKGARQGQHSSQHHGHSPLEQHSPPPPPPVPQQQEPQPERLSPAPLAPPPHPEHPGSTRHSSEDSDITSLIEAMDKDFEHHDAPPLDVFTEQPPSPLSKSKGKGKPPQRKVKPPKKQEEKEKKGKGRALEDELKDSLADDDSSSTTTETSNPDTEPLLKEDTEKQKGKQAMPEKHENEMSQVKQKSKKVLNVKKEIPADVKPSCLELPYTAPLESKQRRNLPTKIPLPTTLTSGSKPRNSQKTKGTSKLVDSRPPALAKFLPNSQELGNTSSSEGEKDSPPPEWDSVPVHKPGSSTDSLYKLSLQTLNADIFLKQRQTSPPPTSPSPPAAPCPFTSRGSYSSIVHGSPGSDSKAKQPSGSKHKLTKAASLPGKNGNPTFAAVTAGYDKSPGGNGFAKVSSSKTDFSSSLGISHTPVDSDGSDSSGLWSPVSNPSSPDFTPLNSFSAFGNSFNLTGEVFSKLGLSRSCNQPSQRSWNELSSGPSYLWDSPATEPSPSWPASSSSPTHTATPILGSTSSLWSSTPFSSSIWSSSLHSTLPFTSPTSALPSISLMGPESAPAAHAPSAASPSDDLGQTYNPWRIWSPTIGRRSSDPWSNSHFPHEN

Target ORF information:

RefSeq Version	XM_015465285.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transmembrane protein 131 (TMEM131), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465285.1

ORF Insert Sequence:

ATGCCAGACA TTTCAGATTT TACTTTGTTG GGTGCGGTGT TTTGGATTCC AAGGAGTATT 
CTTGAAAGAG CTTTGTTTTC TGATGCAGCA TTTATTCAGT CGGAGAGCAT AATAGAAGTG 
CTGCGTTTTG ATGATGGAGG GCTTCTACAG ACTGAGACAA CACTTGGACT CAGTTCATAC 
CAACAGAAAA GTATATCTTT GTACCGGGGT AATTGCAGAC CTATACGGTT TGAGCCACCA 
ATGCTGGATT TCCATGAACA ACCAGTTGGA ATGCCAAAAA TGGAAAAAGT TTACTTACAT 
AATCCTAGTT CTGAAGAAAC TATTACTTTA GTATCAATAT CTGCTACCAC ATCACATTTT 
CACGCATCAT TTTTTCAAAA TAGGAAAATT CTTCCAGGAG GAAATACATC ATTTGATGTA 
GTTTTTCTTG CAAGAGTAGT GGGAAATGTA GAAAATACTT TATTTATTAA TACATCTAAT 
CATGGGGTAT TCACTTACCA GGTATTTGGT GTTGGAGTTC CAAATCCATA CCGATTGAGG 
CCATTCCTTG GGGCCAGAGT CCCTGTGAAT AGCAGTTTCT CACCCATAAT AAACATACAC 
AATCCTCACA GCGAGCCTTT ACAGGTTGTA GAAATGTACT CTAGTGGAGG AGACCTTCAC 
CTTGAACTTC CCACAGGGCA GCAGGGAGGC ACCAGGAAGC TGTGGGAAAT TCCTCCTTAT 
GAAACCAAGG GAGTGATGAG AGCCAGTTTT TCATCTAGAG AAGCAGATAA TCACACAGCT 
TTCATAAGAA TAAAGACAAA TGCTTCAGAC AGCACGGAGT TTATCATCCT TCCGGTTGAA 
GTGGAAGTCA CAACAGCTCC TGGAATTTAT TCCTCAACTG AAATGTTAGA TTTTGGCACA 
CTGCGAACAC AAGATCTACC AAAAGTTTTA AATCTTCATT TATTAAATTC AGGAACAAAA 
GATGTACCAA TAACAAGTGT TCGACCTACA CCACAAAATG ATGCTGTCAC TGTACACTTT 
AAACCAATTA CATTAAAAGC TTCAGAAAGT AAATATACCA AGGTAGCAAG TATTAGTTTT 
GATGCATCAA AGGCGAAAAA ACCATCTCAG TTTTCTGGGA AAATAACTGT GAAAGCAAAG 
GAAAAGAGTT ATTCTAAACT TGAAATACCA TATCAAGCAG AGGTTTTAGA TGGTTATTTG 
GGATTTGATC ACGCTGCAAC ATTATTTCAC ATTCGAGACA GTCCTGCTGA TCCTGTGGAA 
AGGCCAATTT ACCTTACTAA CACTTTCAGC TTTGCGATCC TCATTCATGA TGTGTTGCTA 
CCAGAAGAAG CCAAAACAAT GTTTAAAGTT CACAACTTCA GCAAACCAGT CTTAATTCTA 
CCAAATGGAT CAGGCTACAT TTTTACTCTA TTTTTTATGC CATCCACATC GTCCATGCAC 
ATAGATAACA ATATTTTACT TATTACCAAT GCCTCTAAGT TTCACTTACC TGTGCGGGTA 
TATACAGGCT TTTTAGATTA CTTTGTATTA CCCCCCAAAA TAGAAGAACG TTTCATAGAT 
TTTGGTATAC TGAGTGCAAC AGAAGCAAGT AATATTTTAT TTGCCATTAT AAACAGCAAT 
CCAATTGAGT TGGCTATAAA AAGCTGGCAT ATTATAGGAG ATGGTTTATC AATAGAACTC 
GTAGCTACTG AAAGAGGCAA TAGAACTACA ATCATCTCAA GCCTTCCAGA GTTTGAAAAA 
TCCTCTCTAT CAGACCAGTC ATCGGTAATA CTAGCTTCAG GATACTTTGC AGTGTTCAGA 
GTCAAACTTA CTGCAAAAAA ACTGGAAGGA ATTCATGATG GGGCTATACA GATCACGACA 
GACTATGAGA TCCTAACAAT TCCTGTGAAG GCTGTGATCG CAGTGGGTTC TTTGACTTGC 
TTCCCAAAGC ACATGGTTCT TCCACCTTCC TTTCCAGGGA AAATAGTTCA TCAGAGTTTA 
AATATTATGA ATTCCTTCTC ACAGAAGGTA AAGATCCAAC AGATACGATC TTTGTCAGAA 
GACGTGCGTT TTTACTACAA ACGATTGCGG GGTAATAAGG AAGACTTGGA GCCAGGGAAA 
AAATCAAAGA TTGCAAACAT TTACTTTGAT CCTGGATTGC AGTGCGGGGA TCATTGCTAT 
GTCGGCTTGC CTTTTCTGTC CAAATCTGAA CCCAGAGTCC AGCCTGGCAT AGCCATGCAG 
GAAGATGCCT GGGACACCGA CTGGGATTTG CATCAAAGCC TATTCAAGGG ATGGATGGGA 
ATAAAGGAAA ACTCAGGTCA CAAATTGAGC GCTATGTTTG AAGTGAATAC AGACCTTCAG 
AAAAACATCC TGTCAAAAGT TTCTGCCGAG CTCTCCTGGC CATCCCTCCT CAGCTCCCCC 
CGGCTCGTGA GGTTTCCCCT CACTAATACA AACTGCTCTT CAGAAGAAGA GATTATGTTA 
GAAAACCCTG CAGATGTTCC CATCTATATT CAATTTATCC CCCTGGCTTT ATATTCCAGC 
CCTTCCATCT TTGTAGATAA GTTAATATCA AGGTTTAACT TAAGTAAGGT GGCAAAGATC 
GATTTAAGAA CACAAGAATT CCAAGTCTTT AGAAACACTG CTCACCCACT GCAGAGTTCA 
ACAGGATTCA CAGAGGGCCT CTCCCGACAT TTAGTTTTAA ACTTAATTTT AAAACCTGGA 
GAAAAGAAAT CTGTCAAAGT AAAGTTTACT CCAGTTCACA ATAGAACTGT TTCTTCCCTA 
ATCATAATCA GAAATAACCT GACTGTGATG GATGCCGTAA TGGTCCAAGG ACAGGGGACA 
ACCGAGAACC TGAGGGTGGC AGGCAAGCTT CCGGGCCCAG GGAGCTCCTT ACGCTTCAAA 
ATCACAGAAG CACTGCTGAA AGACTGTACG GAGGGTATGA GACTAAGGGA ACCCAATTTC 
ACATTGAAAA GAACATTTAA AGTAGAGAAC ACAGGACAAC TTCCAATTCA CATAGAAACC 
ATTGAAGTCA GTGGGTACTC TTGTGAGGGA TATGGCTTTA AAGTCGTCAA TTGTCAAGAG 
TTTGCACTGA GCGCCAATGC CTCTAAAGAC ATAGTCATTC TGTTTACTCC TGATTTCACG 
GCTTCTAGAG TCATCCGTGA ACTGAAGTTT ATAACAGCCA GTGGCTCTGA GTTTGTGTTT 
GTATTGAATG CATCCCTTCC TTACCATATG TTAGCAACCT GTGCAGAAGC CCTGCCCAGG 
CCCAACTGGG AGCTGGCTCT GTACATCATC ATCTCAGGAA TAATGAGTGC GCTGTTTCTC 
TTGGTAATTG GAACAGCCTA TTTAGAAGCT CAAGGAATTT GGGAGCCCTT CCGAAGGCGG 
CTGTCCTTTG AGGCCTCGAA CCCGCCCTTT GATGTGGGGA GACCATTTGA TCTCAGGAGA 
ATCGTTGGTA TTTCATCTGA AGGAAACTTG AACACACTCA GCTGTGACCC CGGTCACAGT 
CGGGGCTTCT GTGGAGCAGG TGGCTCGGCC TCCCGGCCCG GCGCAGGGAG TCACAAGCAG 
TGTAGCCCGT CGGTCCACCC GCACAGCAGT CACAGCAATA GAAACTCGGC TGACGTGGAA 
AACGTCAGAG CCAAAAACAG TGCGAGTGCC TCTGGCAGGA CTAATGCCCA GTCGGCGAGC 
AAAGCCAGCC CACTGGTCTT CGAGTCCCAC AGCATGGCCC AGGGTCACAC CGCAGGCCGG 
AAGTCCAAGG GGGCCAGACA GGGCCAGCAC AGCAGCCAGC ACCATGGCCA CAGCCCCCTG 
GAACAGCACT CGCCCCCGCC GCCGCCGCCA GTGCCTCAGC AGCAGGAGCC ACAGCCCGAG 
AGGCTGTCCC CTGCGCCCCT CGCACCCCCG CCCCACCCAG AGCACCCCGG CAGCACCCGG 
CACAGCTCCG AGGACTCAGA CATCACCAGT CTCATCGAAG CCATGGACAA AGACTTCGAG 
CACCACGACG CCCCGCCCCT AGATGTGTTT ACAGAGCAGC CGCCTTCACC CTTGTCGAAA 
AGCAAAGGGA AAGGAAAGCC TCCTCAGCGC AAGGTGAAGC CCCCCAAGAA GCAGGAGGAG 
AAGGAGAAGA AGGGAAAGGG GAGGGCCCTG GAAGACGAGC TGAAGGACTC CTTGGCTGAC 
GACGACAGCT CCTCCACCAC CACAGAGACC TCCAACCCAG ACACAGAGCC TCTCCTCAAG 
GAGGATACAG AAAAGCAAAA GGGAAAACAA GCCATGCCTG AAAAACATGA AAACGAAATG 
TCTCAAGTGA AACAAAAAAG CAAAAAAGTC TTAAATGTTA AGAAAGAAAT CCCAGCAGAT 
GTGAAACCCA GTTGCTTAGA GCTACCGTAT ACTGCCCCCT TGGAAAGCAA ACAGCGCCGA 
AATCTCCCAA CCAAGATTCC TCTTCCAACT ACACTGACCA GTGGATCCAA ACCACGAAAC 
TCCCAGAAAA CAAAAGGTAC AAGTAAGTTA GTGGATAGCA GGCCACCTGC CCTCGCAAAG 
TTTCTCCCAA ACAGTCAAGA ACTAGGCAAC ACCAGCAGCT CAGAAGGTGA AAAAGACTCT 
CCACCACCAG AGTGGGATTC CGTGCCTGTT CACAAGCCCG GCAGCTCTAC AGATAGTCTT 
TATAAACTCT CTCTGCAAAC CCTCAACGCA GACATCTTCC TGAAACAACG CCAGACCTCG 
CCGCCGCCCA CCTCCCCCTC ACCGCCCGCC GCACCCTGCC CGTTCACGTC CCGGGGCAGC 
TACAGCAGCA TCGTCCATGG CAGCCCGGGC AGCGACTCTA AAGCAAAGCA GCCAAGCGGG 
AGCAAACACA AACTGACAAA AGCCGCTTCT CTCCCGGGCA AGAATGGCAA CCCCACTTTC 
GCTGCGGTCA CGGCTGGGTA TGACAAGAGC CCAGGTGGGA ACGGCTTTGC CAAAGTTTCT 
TCAAGCAAAA CAGATTTCTC TAGCAGCCTT GGCATTTCAC ACACTCCTGT TGACAGCGAT 
GGCTCAGACA GCTCGGGTCT GTGGAGCCCC GTGAGCAACC CCAGCAGCCC TGACTTCACA 
CCCCTCAACT CGTTCTCAGC CTTTGGGAAT TCTTTTAACC TAACTGGTGA AGTGTTCAGC 
AAGCTCGGGT TATCGCGATC ATGCAATCAG CCCTCACAGA GGAGCTGGAA TGAGCTGAGC 
AGCGGCCCCT CCTACCTCTG GGACTCGCCG GCCACAGAGC CCAGCCCTTC CTGGCCAGCC 
AGTTCCAGCT CCCCGACCCA CACGGCCACA CCCATCCTTG GCAGCACCAG CAGCCTGTGG 
TCGTCCACTC CGTTCAGCAG CTCCATCTGG TCCAGCAGCC TGCACAGCAC CCTGCCCTTC 
ACCAGCCCCA CCAGCGCGTT GCCGAGCATC AGCCTCATGG GCCCCGAGAG CGCCCCGGCA 
GCTCACGCGC CCTCTGCCGC CAGCCCCTCC GACGACCTGG GCCAGACCTA CAACCCCTGG 
CGGATATGGA GTCCCACCAT CGGACGGAGG AGCTCAGACC CCTGGTCGAA TTCGCACTTC 
CCTCACGAGA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM131 ( XM_015473357.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328843.1 Bos taurus transmembrane protein 131 (TMEM131), transcript variant X3, mRNA.

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CloneID	OBa91812
Clone ID Related Accession (Same CDS sequence)	XM_015465285.1 , XM_015473357.1
Accession Version	XM_015473357.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transmembrane protein 131 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGACA TTTCAGATTT TACTTTGTTG GGTGCGGTGT TTTGGATTCC AAGGAGTATT 
CTTGAAAGAG CTTTGTTTTC TGATGCAGCA TTTATTCAGT CGGAGAGCAT AATAGAAGTG 
CTGCGTTTTG ATGATGGAGG GCTTCTACAG ACTGAGACAA CACTTGGACT CAGTTCATAC 
CAACAGAAAA GTATATCTTT GTACCGGGGT AATTGCAGAC CTATACGGTT TGAGCCACCA 
ATGCTGGATT TCCATGAACA ACCAGTTGGA ATGCCAAAAA TGGAAAAAGT TTACTTACAT 
AATCCTAGTT CTGAAGAAAC TATTACTTTA GTATCAATAT CTGCTACCAC ATCACATTTT 
CACGCATCAT TTTTTCAAAA TAGGAAAATT CTTCCAGGAG GAAATACATC ATTTGATGTA 
GTTTTTCTTG CAAGAGTAGT GGGAAATGTA GAAAATACTT TATTTATTAA TACATCTAAT 
CATGGGGTAT TCACTTACCA GGTATTTGGT GTTGGAGTTC CAAATCCATA CCGATTGAGG 
CCATTCCTTG GGGCCAGAGT CCCTGTGAAT AGCAGTTTCT CACCCATAAT AAACATACAC 
AATCCTCACA GCGAGCCTTT ACAGGTTGTA GAAATGTACT CTAGTGGAGG AGACCTTCAC 
CTTGAACTTC CCACAGGGCA GCAGGGAGGC ACCAGGAAGC TGTGGGAAAT TCCTCCTTAT 
GAAACCAAGG GAGTGATGAG AGCCAGTTTT TCATCTAGAG AAGCAGATAA TCACACAGCT 
TTCATAAGAA TAAAGACAAA TGCTTCAGAC AGCACGGAGT TTATCATCCT TCCGGTTGAA 
GTGGAAGTCA CAACAGCTCC TGGAATTTAT TCCTCAACTG AAATGTTAGA TTTTGGCACA 
CTGCGAACAC AAGATCTACC AAAAGTTTTA AATCTTCATT TATTAAATTC AGGAACAAAA 
GATGTACCAA TAACAAGTGT TCGACCTACA CCACAAAATG ATGCTGTCAC TGTACACTTT 
AAACCAATTA CATTAAAAGC TTCAGAAAGT AAATATACCA AGGTAGCAAG TATTAGTTTT 
GATGCATCAA AGGCGAAAAA ACCATCTCAG TTTTCTGGGA AAATAACTGT GAAAGCAAAG 
GAAAAGAGTT ATTCTAAACT TGAAATACCA TATCAAGCAG AGGTTTTAGA TGGTTATTTG 
GGATTTGATC ACGCTGCAAC ATTATTTCAC ATTCGAGACA GTCCTGCTGA TCCTGTGGAA 
AGGCCAATTT ACCTTACTAA CACTTTCAGC TTTGCGATCC TCATTCATGA TGTGTTGCTA 
CCAGAAGAAG CCAAAACAAT GTTTAAAGTT CACAACTTCA GCAAACCAGT CTTAATTCTA 
CCAAATGGAT CAGGCTACAT TTTTACTCTA TTTTTTATGC CATCCACATC GTCCATGCAC 
ATAGATAACA ATATTTTACT TATTACCAAT GCCTCTAAGT TTCACTTACC TGTGCGGGTA 
TATACAGGCT TTTTAGATTA CTTTGTATTA CCCCCCAAAA TAGAAGAACG TTTCATAGAT 
TTTGGTATAC TGAGTGCAAC AGAAGCAAGT AATATTTTAT TTGCCATTAT AAACAGCAAT 
CCAATTGAGT TGGCTATAAA AAGCTGGCAT ATTATAGGAG ATGGTTTATC AATAGAACTC 
GTAGCTACTG AAAGAGGCAA TAGAACTACA ATCATCTCAA GCCTTCCAGA GTTTGAAAAA 
TCCTCTCTAT CAGACCAGTC ATCGGTAATA CTAGCTTCAG GATACTTTGC AGTGTTCAGA 
GTCAAACTTA CTGCAAAAAA ACTGGAAGGA ATTCATGATG GGGCTATACA GATCACGACA 
GACTATGAGA TCCTAACAAT TCCTGTGAAG GCTGTGATCG CAGTGGGTTC TTTGACTTGC 
TTCCCAAAGC ACATGGTTCT TCCACCTTCC TTTCCAGGGA AAATAGTTCA TCAGAGTTTA 
AATATTATGA ATTCCTTCTC ACAGAAGGTA AAGATCCAAC AGATACGATC TTTGTCAGAA 
GACGTGCGTT TTTACTACAA ACGATTGCGG GGTAATAAGG AAGACTTGGA GCCAGGGAAA 
AAATCAAAGA TTGCAAACAT TTACTTTGAT CCTGGATTGC AGTGCGGGGA TCATTGCTAT 
GTCGGCTTGC CTTTTCTGTC CAAATCTGAA CCCAGAGTCC AGCCTGGCAT AGCCATGCAG 
GAAGATGCCT GGGACACCGA CTGGGATTTG CATCAAAGCC TATTCAAGGG ATGGATGGGA 
ATAAAGGAAA ACTCAGGTCA CAAATTGAGC GCTATGTTTG AAGTGAATAC AGACCTTCAG 
AAAAACATCC TGTCAAAAGT TTCTGCCGAG CTCTCCTGGC CATCCCTCCT CAGCTCCCCC 
CGGCTCGTGA GGTTTCCCCT CACTAATACA AACTGCTCTT CAGAAGAAGA GATTATGTTA 
GAAAACCCTG CAGATGTTCC CATCTATATT CAATTTATCC CCCTGGCTTT ATATTCCAGC 
CCTTCCATCT TTGTAGATAA GTTAATATCA AGGTTTAACT TAAGTAAGGT GGCAAAGATC 
GATTTAAGAA CACAAGAATT CCAAGTCTTT AGAAACACTG CTCACCCACT GCAGAGTTCA 
ACAGGATTCA CAGAGGGCCT CTCCCGACAT TTAGTTTTAA ACTTAATTTT AAAACCTGGA 
GAAAAGAAAT CTGTCAAAGT AAAGTTTACT CCAGTTCACA ATAGAACTGT TTCTTCCCTA 
ATCATAATCA GAAATAACCT GACTGTGATG GATGCCGTAA TGGTCCAAGG ACAGGGGACA 
ACCGAGAACC TGAGGGTGGC AGGCAAGCTT CCGGGCCCAG GGAGCTCCTT ACGCTTCAAA 
ATCACAGAAG CACTGCTGAA AGACTGTACG GAGGGTATGA GACTAAGGGA ACCCAATTTC 
ACATTGAAAA GAACATTTAA AGTAGAGAAC ACAGGACAAC TTCCAATTCA CATAGAAACC 
ATTGAAGTCA GTGGGTACTC TTGTGAGGGA TATGGCTTTA AAGTCGTCAA TTGTCAAGAG 
TTTGCACTGA GCGCCAATGC CTCTAAAGAC ATAGTCATTC TGTTTACTCC TGATTTCACG 
GCTTCTAGAG TCATCCGTGA ACTGAAGTTT ATAACAGCCA GTGGCTCTGA GTTTGTGTTT 
GTATTGAATG CATCCCTTCC TTACCATATG TTAGCAACCT GTGCAGAAGC CCTGCCCAGG 
CCCAACTGGG AGCTGGCTCT GTACATCATC ATCTCAGGAA TAATGAGTGC GCTGTTTCTC 
TTGGTAATTG GAACAGCCTA TTTAGAAGCT CAAGGAATTT GGGAGCCCTT CCGAAGGCGG 
CTGTCCTTTG AGGCCTCGAA CCCGCCCTTT GATGTGGGGA GACCATTTGA TCTCAGGAGA 
ATCGTTGGTA TTTCATCTGA AGGAAACTTG AACACACTCA GCTGTGACCC CGGTCACAGT 
CGGGGCTTCT GTGGAGCAGG TGGCTCGGCC TCCCGGCCCG GCGCAGGGAG TCACAAGCAG 
TGTAGCCCGT CGGTCCACCC GCACAGCAGT CACAGCAATA GAAACTCGGC TGACGTGGAA 
AACGTCAGAG CCAAAAACAG TGCGAGTGCC TCTGGCAGGA CTAATGCCCA GTCGGCGAGC 
AAAGCCAGCC CACTGGTCTT CGAGTCCCAC AGCATGGCCC AGGGTCACAC CGCAGGCCGG 
AAGTCCAAGG GGGCCAGACA GGGCCAGCAC AGCAGCCAGC ACCATGGCCA CAGCCCCCTG 
GAACAGCACT CGCCCCCGCC GCCGCCGCCA GTGCCTCAGC AGCAGGAGCC ACAGCCCGAG 
AGGCTGTCCC CTGCGCCCCT CGCACCCCCG CCCCACCCAG AGCACCCCGG CAGCACCCGG 
CACAGCTCCG AGGACTCAGA CATCACCAGT CTCATCGAAG CCATGGACAA AGACTTCGAG 
CACCACGACG CCCCGCCCCT AGATGTGTTT ACAGAGCAGC CGCCTTCACC CTTGTCGAAA 
AGCAAAGGGA AAGGAAAGCC TCCTCAGCGC AAGGTGAAGC CCCCCAAGAA GCAGGAGGAG 
AAGGAGAAGA AGGGAAAGGG GAGGGCCCTG GAAGACGAGC TGAAGGACTC CTTGGCTGAC 
GACGACAGCT CCTCCACCAC CACAGAGACC TCCAACCCAG ACACAGAGCC TCTCCTCAAG 
GAGGATACAG AAAAGCAAAA GGGAAAACAA GCCATGCCTG AAAAACATGA AAACGAAATG 
TCTCAAGTGA AACAAAAAAG CAAAAAAGTC TTAAATGTTA AGAAAGAAAT CCCAGCAGAT 
GTGAAACCCA GTTGCTTAGA GCTACCGTAT ACTGCCCCCT TGGAAAGCAA ACAGCGCCGA 
AATCTCCCAA CCAAGATTCC TCTTCCAACT ACACTGACCA GTGGATCCAA ACCACGAAAC 
TCCCAGAAAA CAAAAGGTAC AAGTAAGTTA GTGGATAGCA GGCCACCTGC CCTCGCAAAG 
TTTCTCCCAA ACAGTCAAGA ACTAGGCAAC ACCAGCAGCT CAGAAGGTGA AAAAGACTCT 
CCACCACCAG AGTGGGATTC CGTGCCTGTT CACAAGCCCG GCAGCTCTAC AGATAGTCTT 
TATAAACTCT CTCTGCAAAC CCTCAACGCA GACATCTTCC TGAAACAACG CCAGACCTCG 
CCGCCGCCCA CCTCCCCCTC ACCGCCCGCC GCACCCTGCC CGTTCACGTC CCGGGGCAGC 
TACAGCAGCA TCGTCCATGG CAGCCCGGGC AGCGACTCTA AAGCAAAGCA GCCAAGCGGG 
AGCAAACACA AACTGACAAA AGCCGCTTCT CTCCCGGGCA AGAATGGCAA CCCCACTTTC 
GCTGCGGTCA CGGCTGGGTA TGACAAGAGC CCAGGTGGGA ACGGCTTTGC CAAAGTTTCT 
TCAAGCAAAA CAGATTTCTC TAGCAGCCTT GGCATTTCAC ACACTCCTGT TGACAGCGAT 
GGCTCAGACA GCTCGGGTCT GTGGAGCCCC GTGAGCAACC CCAGCAGCCC TGACTTCACA 
CCCCTCAACT CGTTCTCAGC CTTTGGGAAT TCTTTTAACC TAACTGGTGA AGTGTTCAGC 
AAGCTCGGGT TATCGCGATC ATGCAATCAG CCCTCACAGA GGAGCTGGAA TGAGCTGAGC 
AGCGGCCCCT CCTACCTCTG GGACTCGCCG GCCACAGAGC CCAGCCCTTC CTGGCCAGCC 
AGTTCCAGCT CCCCGACCCA CACGGCCACA CCCATCCTTG GCAGCACCAG CAGCCTGTGG 
TCGTCCACTC CGTTCAGCAG CTCCATCTGG TCCAGCAGCC TGCACAGCAC CCTGCCCTTC 
ACCAGCCCCA CCAGCGCGTT GCCGAGCATC AGCCTCATGG GCCCCGAGAG CGCCCCGGCA 
GCTCACGCGC CCTCTGCCGC CAGCCCCTCC GACGACCTGG GCCAGACCTA CAACCCCTGG 
CGGATATGGA GTCCCACCAT CGGACGGAGG AGCTCAGACC CCTGGTCGAA TTCGCACTTC 
CCTCACGAGA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328843.1
CDS	16..5550
Translation	MPDISDFTLLGAVFWIPRSILERALFSDAAFIQSESIIEVLRFDDGGLLQTETTLGLSSYQQKSISLYRGNCRPIRFEPPMLDFHEQPVGMPKMEKVYLHNPSSEETITLVSISATTSHFHASFFQNRKILPGGNTSFDVVFLARVVGNVENTLFINTSNHGVFTYQVFGVGVPNPYRLRPFLGARVPVNSSFSPIINIHNPHSEPLQVVEMYSSGGDLHLELPTGQQGGTRKLWEIPPYETKGVMRASFSSREADNHTAFIRIKTNASDSTEFIILPVEVEVTTAPGIYSSTEMLDFGTLRTQDLPKVLNLHLLNSGTKDVPITSVRPTPQNDAVTVHFKPITLKASESKYTKVASISFDASKAKKPSQFSGKITVKAKEKSYSKLEIPYQAEVLDGYLGFDHAATLFHIRDSPADPVERPIYLTNTFSFAILIHDVLLPEEAKTMFKVHNFSKPVLILPNGSGYIFTLFFMPSTSSMHIDNNILLITNASKFHLPVRVYTGFLDYFVLPPKIEERFIDFGILSATEASNILFAIINSNPIELAIKSWHIIGDGLSIELVATERGNRTTIISSLPEFEKSSLSDQSSVILASGYFAVFRVKLTAKKLEGIHDGAIQITTDYEILTIPVKAVIAVGSLTCFPKHMVLPPSFPGKIVHQSLNIMNSFSQKVKIQQIRSLSEDVRFYYKRLRGNKEDLEPGKKSKIANIYFDPGLQCGDHCYVGLPFLSKSEPRVQPGIAMQEDAWDTDWDLHQSLFKGWMGIKENSGHKLSAMFEVNTDLQKNILSKVSAELSWPSLLSSPRLVRFPLTNTNCSSEEEIMLENPADVPIYIQFIPLALYSSPSIFVDKLISRFNLSKVAKIDLRTQEFQVFRNTAHPLQSSTGFTEGLSRHLVLNLILKPGEKKSVKVKFTPVHNRTVSSLIIIRNNLTVMDAVMVQGQGTTENLRVAGKLPGPGSSLRFKITEALLKDCTEGMRLREPNFTLKRTFKVENTGQLPIHIETIEVSGYSCEGYGFKVVNCQEFALSANASKDIVILFTPDFTASRVIRELKFITASGSEFVFVLNASLPYHMLATCAEALPRPNWELALYIIISGIMSALFLLVIGTAYLEAQGIWEPFRRRLSFEASNPPFDVGRPFDLRRIVGISSEGNLNTLSCDPGHSRGFCGAGGSASRPGAGSHKQCSPSVHPHSSHSNRNSADVENVRAKNSASASGRTNAQSASKASPLVFESHSMAQGHTAGRKSKGARQGQHSSQHHGHSPLEQHSPPPPPPVPQQQEPQPERLSPAPLAPPPHPEHPGSTRHSSEDSDITSLIEAMDKDFEHHDAPPLDVFTEQPPSPLSKSKGKGKPPQRKVKPPKKQEEKEKKGKGRALEDELKDSLADDDSSSTTTETSNPDTEPLLKEDTEKQKGKQAMPEKHENEMSQVKQKSKKVLNVKKEIPADVKPSCLELPYTAPLESKQRRNLPTKIPLPTTLTSGSKPRNSQKTKGTSKLVDSRPPALAKFLPNSQELGNTSSSEGEKDSPPPEWDSVPVHKPGSSTDSLYKLSLQTLNADIFLKQRQTSPPPTSPSPPAAPCPFTSRGSYSSIVHGSPGSDSKAKQPSGSKHKLTKAASLPGKNGNPTFAAVTAGYDKSPGGNGFAKVSSSKTDFSSSLGISHTPVDSDGSDSSGLWSPVSNPSSPDFTPLNSFSAFGNSFNLTGEVFSKLGLSRSCNQPSQRSWNELSSGPSYLWDSPATEPSPSWPASSSSPTHTATPILGSTSSLWSSTPFSSSIWSSSLHSTLPFTSPTSALPSISLMGPESAPAAHAPSAASPSDDLGQTYNPWRIWSPTIGRRSSDPWSNSHFPHEN

Target ORF information:

RefSeq Version	XM_015473357.1
Organism	Bos taurus(cattle)
Definition	Bos taurus transmembrane protein 131 (TMEM131), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473357.1

ORF Insert Sequence:

ATGCCAGACA TTTCAGATTT TACTTTGTTG GGTGCGGTGT TTTGGATTCC AAGGAGTATT 
CTTGAAAGAG CTTTGTTTTC TGATGCAGCA TTTATTCAGT CGGAGAGCAT AATAGAAGTG 
CTGCGTTTTG ATGATGGAGG GCTTCTACAG ACTGAGACAA CACTTGGACT CAGTTCATAC 
CAACAGAAAA GTATATCTTT GTACCGGGGT AATTGCAGAC CTATACGGTT TGAGCCACCA 
ATGCTGGATT TCCATGAACA ACCAGTTGGA ATGCCAAAAA TGGAAAAAGT TTACTTACAT 
AATCCTAGTT CTGAAGAAAC TATTACTTTA GTATCAATAT CTGCTACCAC ATCACATTTT 
CACGCATCAT TTTTTCAAAA TAGGAAAATT CTTCCAGGAG GAAATACATC ATTTGATGTA 
GTTTTTCTTG CAAGAGTAGT GGGAAATGTA GAAAATACTT TATTTATTAA TACATCTAAT 
CATGGGGTAT TCACTTACCA GGTATTTGGT GTTGGAGTTC CAAATCCATA CCGATTGAGG 
CCATTCCTTG GGGCCAGAGT CCCTGTGAAT AGCAGTTTCT CACCCATAAT AAACATACAC 
AATCCTCACA GCGAGCCTTT ACAGGTTGTA GAAATGTACT CTAGTGGAGG AGACCTTCAC 
CTTGAACTTC CCACAGGGCA GCAGGGAGGC ACCAGGAAGC TGTGGGAAAT TCCTCCTTAT 
GAAACCAAGG GAGTGATGAG AGCCAGTTTT TCATCTAGAG AAGCAGATAA TCACACAGCT 
TTCATAAGAA TAAAGACAAA TGCTTCAGAC AGCACGGAGT TTATCATCCT TCCGGTTGAA 
GTGGAAGTCA CAACAGCTCC TGGAATTTAT TCCTCAACTG AAATGTTAGA TTTTGGCACA 
CTGCGAACAC AAGATCTACC AAAAGTTTTA AATCTTCATT TATTAAATTC AGGAACAAAA 
GATGTACCAA TAACAAGTGT TCGACCTACA CCACAAAATG ATGCTGTCAC TGTACACTTT 
AAACCAATTA CATTAAAAGC TTCAGAAAGT AAATATACCA AGGTAGCAAG TATTAGTTTT 
GATGCATCAA AGGCGAAAAA ACCATCTCAG TTTTCTGGGA AAATAACTGT GAAAGCAAAG 
GAAAAGAGTT ATTCTAAACT TGAAATACCA TATCAAGCAG AGGTTTTAGA TGGTTATTTG 
GGATTTGATC ACGCTGCAAC ATTATTTCAC ATTCGAGACA GTCCTGCTGA TCCTGTGGAA 
AGGCCAATTT ACCTTACTAA CACTTTCAGC TTTGCGATCC TCATTCATGA TGTGTTGCTA 
CCAGAAGAAG CCAAAACAAT GTTTAAAGTT CACAACTTCA GCAAACCAGT CTTAATTCTA 
CCAAATGGAT CAGGCTACAT TTTTACTCTA TTTTTTATGC CATCCACATC GTCCATGCAC 
ATAGATAACA ATATTTTACT TATTACCAAT GCCTCTAAGT TTCACTTACC TGTGCGGGTA 
TATACAGGCT TTTTAGATTA CTTTGTATTA CCCCCCAAAA TAGAAGAACG TTTCATAGAT 
TTTGGTATAC TGAGTGCAAC AGAAGCAAGT AATATTTTAT TTGCCATTAT AAACAGCAAT 
CCAATTGAGT TGGCTATAAA AAGCTGGCAT ATTATAGGAG ATGGTTTATC AATAGAACTC 
GTAGCTACTG AAAGAGGCAA TAGAACTACA ATCATCTCAA GCCTTCCAGA GTTTGAAAAA 
TCCTCTCTAT CAGACCAGTC ATCGGTAATA CTAGCTTCAG GATACTTTGC AGTGTTCAGA 
GTCAAACTTA CTGCAAAAAA ACTGGAAGGA ATTCATGATG GGGCTATACA GATCACGACA 
GACTATGAGA TCCTAACAAT TCCTGTGAAG GCTGTGATCG CAGTGGGTTC TTTGACTTGC 
TTCCCAAAGC ACATGGTTCT TCCACCTTCC TTTCCAGGGA AAATAGTTCA TCAGAGTTTA 
AATATTATGA ATTCCTTCTC ACAGAAGGTA AAGATCCAAC AGATACGATC TTTGTCAGAA 
GACGTGCGTT TTTACTACAA ACGATTGCGG GGTAATAAGG AAGACTTGGA GCCAGGGAAA 
AAATCAAAGA TTGCAAACAT TTACTTTGAT CCTGGATTGC AGTGCGGGGA TCATTGCTAT 
GTCGGCTTGC CTTTTCTGTC CAAATCTGAA CCCAGAGTCC AGCCTGGCAT AGCCATGCAG 
GAAGATGCCT GGGACACCGA CTGGGATTTG CATCAAAGCC TATTCAAGGG ATGGATGGGA 
ATAAAGGAAA ACTCAGGTCA CAAATTGAGC GCTATGTTTG AAGTGAATAC AGACCTTCAG 
AAAAACATCC TGTCAAAAGT TTCTGCCGAG CTCTCCTGGC CATCCCTCCT CAGCTCCCCC 
CGGCTCGTGA GGTTTCCCCT CACTAATACA AACTGCTCTT CAGAAGAAGA GATTATGTTA 
GAAAACCCTG CAGATGTTCC CATCTATATT CAATTTATCC CCCTGGCTTT ATATTCCAGC 
CCTTCCATCT TTGTAGATAA GTTAATATCA AGGTTTAACT TAAGTAAGGT GGCAAAGATC 
GATTTAAGAA CACAAGAATT CCAAGTCTTT AGAAACACTG CTCACCCACT GCAGAGTTCA 
ACAGGATTCA CAGAGGGCCT CTCCCGACAT TTAGTTTTAA ACTTAATTTT AAAACCTGGA 
GAAAAGAAAT CTGTCAAAGT AAAGTTTACT CCAGTTCACA ATAGAACTGT TTCTTCCCTA 
ATCATAATCA GAAATAACCT GACTGTGATG GATGCCGTAA TGGTCCAAGG ACAGGGGACA 
ACCGAGAACC TGAGGGTGGC AGGCAAGCTT CCGGGCCCAG GGAGCTCCTT ACGCTTCAAA 
ATCACAGAAG CACTGCTGAA AGACTGTACG GAGGGTATGA GACTAAGGGA ACCCAATTTC 
ACATTGAAAA GAACATTTAA AGTAGAGAAC ACAGGACAAC TTCCAATTCA CATAGAAACC 
ATTGAAGTCA GTGGGTACTC TTGTGAGGGA TATGGCTTTA AAGTCGTCAA TTGTCAAGAG 
TTTGCACTGA GCGCCAATGC CTCTAAAGAC ATAGTCATTC TGTTTACTCC TGATTTCACG 
GCTTCTAGAG TCATCCGTGA ACTGAAGTTT ATAACAGCCA GTGGCTCTGA GTTTGTGTTT 
GTATTGAATG CATCCCTTCC TTACCATATG TTAGCAACCT GTGCAGAAGC CCTGCCCAGG 
CCCAACTGGG AGCTGGCTCT GTACATCATC ATCTCAGGAA TAATGAGTGC GCTGTTTCTC 
TTGGTAATTG GAACAGCCTA TTTAGAAGCT CAAGGAATTT GGGAGCCCTT CCGAAGGCGG 
CTGTCCTTTG AGGCCTCGAA CCCGCCCTTT GATGTGGGGA GACCATTTGA TCTCAGGAGA 
ATCGTTGGTA TTTCATCTGA AGGAAACTTG AACACACTCA GCTGTGACCC CGGTCACAGT 
CGGGGCTTCT GTGGAGCAGG TGGCTCGGCC TCCCGGCCCG GCGCAGGGAG TCACAAGCAG 
TGTAGCCCGT CGGTCCACCC GCACAGCAGT CACAGCAATA GAAACTCGGC TGACGTGGAA 
AACGTCAGAG CCAAAAACAG TGCGAGTGCC TCTGGCAGGA CTAATGCCCA GTCGGCGAGC 
AAAGCCAGCC CACTGGTCTT CGAGTCCCAC AGCATGGCCC AGGGTCACAC CGCAGGCCGG 
AAGTCCAAGG GGGCCAGACA GGGCCAGCAC AGCAGCCAGC ACCATGGCCA CAGCCCCCTG 
GAACAGCACT CGCCCCCGCC GCCGCCGCCA GTGCCTCAGC AGCAGGAGCC ACAGCCCGAG 
AGGCTGTCCC CTGCGCCCCT CGCACCCCCG CCCCACCCAG AGCACCCCGG CAGCACCCGG 
CACAGCTCCG AGGACTCAGA CATCACCAGT CTCATCGAAG CCATGGACAA AGACTTCGAG 
CACCACGACG CCCCGCCCCT AGATGTGTTT ACAGAGCAGC CGCCTTCACC CTTGTCGAAA 
AGCAAAGGGA AAGGAAAGCC TCCTCAGCGC AAGGTGAAGC CCCCCAAGAA GCAGGAGGAG 
AAGGAGAAGA AGGGAAAGGG GAGGGCCCTG GAAGACGAGC TGAAGGACTC CTTGGCTGAC 
GACGACAGCT CCTCCACCAC CACAGAGACC TCCAACCCAG ACACAGAGCC TCTCCTCAAG 
GAGGATACAG AAAAGCAAAA GGGAAAACAA GCCATGCCTG AAAAACATGA AAACGAAATG 
TCTCAAGTGA AACAAAAAAG CAAAAAAGTC TTAAATGTTA AGAAAGAAAT CCCAGCAGAT 
GTGAAACCCA GTTGCTTAGA GCTACCGTAT ACTGCCCCCT TGGAAAGCAA ACAGCGCCGA 
AATCTCCCAA CCAAGATTCC TCTTCCAACT ACACTGACCA GTGGATCCAA ACCACGAAAC 
TCCCAGAAAA CAAAAGGTAC AAGTAAGTTA GTGGATAGCA GGCCACCTGC CCTCGCAAAG 
TTTCTCCCAA ACAGTCAAGA ACTAGGCAAC ACCAGCAGCT CAGAAGGTGA AAAAGACTCT 
CCACCACCAG AGTGGGATTC CGTGCCTGTT CACAAGCCCG GCAGCTCTAC AGATAGTCTT 
TATAAACTCT CTCTGCAAAC CCTCAACGCA GACATCTTCC TGAAACAACG CCAGACCTCG 
CCGCCGCCCA CCTCCCCCTC ACCGCCCGCC GCACCCTGCC CGTTCACGTC CCGGGGCAGC 
TACAGCAGCA TCGTCCATGG CAGCCCGGGC AGCGACTCTA AAGCAAAGCA GCCAAGCGGG 
AGCAAACACA AACTGACAAA AGCCGCTTCT CTCCCGGGCA AGAATGGCAA CCCCACTTTC 
GCTGCGGTCA CGGCTGGGTA TGACAAGAGC CCAGGTGGGA ACGGCTTTGC CAAAGTTTCT 
TCAAGCAAAA CAGATTTCTC TAGCAGCCTT GGCATTTCAC ACACTCCTGT TGACAGCGAT 
GGCTCAGACA GCTCGGGTCT GTGGAGCCCC GTGAGCAACC CCAGCAGCCC TGACTTCACA 
CCCCTCAACT CGTTCTCAGC CTTTGGGAAT TCTTTTAACC TAACTGGTGA AGTGTTCAGC 
AAGCTCGGGT TATCGCGATC ATGCAATCAG CCCTCACAGA GGAGCTGGAA TGAGCTGAGC 
AGCGGCCCCT CCTACCTCTG GGACTCGCCG GCCACAGAGC CCAGCCCTTC CTGGCCAGCC 
AGTTCCAGCT CCCCGACCCA CACGGCCACA CCCATCCTTG GCAGCACCAG CAGCCTGTGG 
TCGTCCACTC CGTTCAGCAG CTCCATCTGG TCCAGCAGCC TGCACAGCAC CCTGCCCTTC 
ACCAGCCCCA CCAGCGCGTT GCCGAGCATC AGCCTCATGG GCCCCGAGAG CGCCCCGGCA 
GCTCACGCGC CCTCTGCCGC CAGCCCCTCC GACGACCTGG GCCAGACCTA CAACCCCTGG 
CGGATATGGA GTCCCACCAT CGGACGGAGG AGCTCAGACC CCTGGTCGAA TTCGCACTTC 
CCTCACGAGA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM131 ( XM_002691141.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002691187.1 Bos taurus transmembrane protein 131 (TMEM131), transcript variant X1, mRNA.

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CloneID	OBa06771
Clone ID Related Accession (Same CDS sequence)	XM_002691141.5 , XM_002691141.6 , XM_613474.9
Accession Version	XM_002691141.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transmembrane protein 131 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002691141.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGC GGGCCGGAGG AGGAGCAGCG GGATCCGCCG CCGCTGCCTC CACGTCCGCC 
GTGGCCGGTC TGGAGCCCGC GGCCGGCCGC GGCGGGGGCC CGCGGAGCGC AGCCGCCGGC 
CTCCTGGGAG CGCTGCACCT GGTGATGACC CTCGTCGTGG CCGCGGCGCG GGCCGAGAAG 
GAAGCATTTA TTCAGTCGGA GAGCATAATA GAAGTGCTGC GTTTTGATGA TGGAGGGCTT 
CTACAGACTG AGACAACACT TGGACTCAGT TCATACCAAC AGAAAAGTAT ATCTTTGTAC 
CGGGGTAATT GCAGACCTAT ACGGTTTGAG CCACCAATGC TGGATTTCCA TGAACAACCA 
GTTGGAATGC CAAAAATGGA AAAAGTTTAC TTACATAATC CTAGTTCTGA AGAAACTATT 
ACTTTAGTAT CAATATCTGC TACCACATCA CATTTTCACG CATCATTTTT TCAAAATAGG 
AAAATTCTTC CAGGAGGAAA TACATCATTT GATGTAGTTT TTCTTGCAAG AGTAGTGGGA 
AATGTAGAAA ATACTTTATT TATTAATACA TCTAATCATG GGGTATTCAC TTACCAGGTA 
TTTGGTGTTG GAGTTCCAAA TCCATACCGA TTGAGGCCAT TCCTTGGGGC CAGAGTCCCT 
GTGAATAGCA GTTTCTCACC CATAATAAAC ATACACAATC CTCACAGCGA GCCTTTACAG 
GTTGTAGAAA TGTACTCTAG TGGAGGAGAC CTTCACCTTG AACTTCCCAC AGGGCAGCAG 
GGAGGCACCA GGAAGCTGTG GGAAATTCCT CCTTATGAAA CCAAGGGAGT GATGAGAGCC 
AGTTTTTCAT CTAGAGAAGC AGATAATCAC ACAGCTTTCA TAAGAATAAA GACAAATGCT 
TCAGACAGCA CGGAGTTTAT CATCCTTCCG GTTGAAGTGG AAGTCACAAC AGCTCCTGGA 
ATTTATTCCT CAACTGAAAT GTTAGATTTT GGCACACTGC GAACACAAGA TCTACCAAAA 
GTTTTAAATC TTCATTTATT AAATTCAGGA ACAAAAGATG TACCAATAAC AAGTGTTCGA 
CCTACACCAC AAAATGATGC TGTCACTGTA CACTTTAAAC CAATTACATT AAAAGCTTCA 
GAAAGTAAAT ATACCAAGGT AGCAAGTATT AGTTTTGATG CATCAAAGGC GAAAAAACCA 
TCTCAGTTTT CTGGGAAAAT AACTGTGAAA GCAAAGGAAA AGAGTTATTC TAAACTTGAA 
ATACCATATC AAGCAGAGGT TTTAGATGGT TATTTGGGAT TTGATCACGC TGCAACATTA 
TTTCACATTC GAGACAGTCC TGCTGATCCT GTGGAAAGGC CAATTTACCT TACTAACACT 
TTCAGCTTTG CGATCCTCAT TCATGATGTG TTGCTACCAG AAGAAGCCAA AACAATGTTT 
AAAGTTCACA ACTTCAGCAA ACCAGTCTTA ATTCTACCAA ATGGATCAGG CTACATTTTT 
ACTCTATTTT TTATGCCATC CACATCGTCC ATGCACATAG ATAACAATAT TTTACTTATT 
ACCAATGCCT CTAAGTTTCA CTTACCTGTG CGGGTATATA CAGGCTTTTT AGATTACTTT 
GTATTACCCC CCAAAATAGA AGAACGTTTC ATAGATTTTG GTATACTGAG TGCAACAGAA 
GCAAGTAATA TTTTATTTGC CATTATAAAC AGCAATCCAA TTGAGTTGGC TATAAAAAGC 
TGGCATATTA TAGGAGATGG TTTATCAATA GAACTCGTAG CTACTGAAAG AGGCAATAGA 
ACTACAATCA TCTCAAGCCT TCCAGAGTTT GAAAAATCCT CTCTATCAGA CCAGTCATCG 
GTAATACTAG CTTCAGGATA CTTTGCAGTG TTCAGAGTCA AACTTACTGC AAAAAAACTG 
GAAGGAATTC ATGATGGGGC TATACAGATC ACGACAGACT ATGAGATCCT AACAATTCCT 
GTGAAGGCTG TGATCGCAGT GGGTTCTTTG ACTTGCTTCC CAAAGCACAT GGTTCTTCCA 
CCTTCCTTTC CAGGGAAAAT AGTTCATCAG AGTTTAAATA TTATGAATTC CTTCTCACAG 
AAGGTAAAGA TCCAACAGAT ACGATCTTTG TCAGAAGACG TGCGTTTTTA CTACAAACGA 
TTGCGGGGTA ATAAGGAAGA CTTGGAGCCA GGGAAAAAAT CAAAGATTGC AAACATTTAC 
TTTGATCCTG GATTGCAGTG CGGGGATCAT TGCTATGTCG GCTTGCCTTT TCTGTCCAAA 
TCTGAACCCA GAGTCCAGCC TGGCATAGCC ATGCAGGAAG ATGCCTGGGA CACCGACTGG 
GATTTGCATC AAAGCCTATT CAAGGGATGG ATGGGAATAA AGGAAAACTC AGGTCACAAA 
TTGAGCGCTA TGTTTGAAGT GAATACAGAC CTTCAGAAAA ACATCCTGTC AAAAGTTTCT 
GCCGAGCTCT CCTGGCCATC CCTCCTCAGC TCCCCCCGGC TCGTGAGGTT TCCCCTCACT 
AATACAAACT GCTCTTCAGA AGAAGAGATT ATGTTAGAAA ACCCTGCAGA TGTTCCCATC 
TATATTCAAT TTATCCCCCT GGCTTTATAT TCCAGCCCTT CCATCTTTGT AGATAAGTTA 
ATATCAAGGT TTAACTTAAG TAAGGTGGCA AAGATCGATT TAAGAACACA AGAATTCCAA 
GTCTTTAGAA ACACTGCTCA CCCACTGCAG AGTTCAACAG GATTCACAGA GGGCCTCTCC 
CGACATTTAG TTTTAAACTT AATTTTAAAA CCTGGAGAAA AGAAATCTGT CAAAGTAAAG 
TTTACTCCAG TTCACAATAG AACTGTTTCT TCCCTAATCA TAATCAGAAA TAACCTGACT 
GTGATGGATG CCGTAATGGT CCAAGGACAG GGGACAACCG AGAACCTGAG GGTGGCAGGC 
AAGCTTCCGG GCCCAGGGAG CTCCTTACGC TTCAAAATCA CAGAAGCACT GCTGAAAGAC 
TGTACGGAGG GTATGAGACT AAGGGAACCC AATTTCACAT TGAAAAGAAC ATTTAAAGTA 
GAGAACACAG GACAACTTCC AATTCACATA GAAACCATTG AAGTCAGTGG GTACTCTTGT 
GAGGGATATG GCTTTAAAGT CGTCAATTGT CAAGAGTTTG CACTGAGCGC CAATGCCTCT 
AAAGACATAG TCATTCTGTT TACTCCTGAT TTCACGGCTT CTAGAGTCAT CCGTGAACTG 
AAGTTTATAA CAGCCAGTGG CTCTGAGTTT GTGTTTGTAT TGAATGCATC CCTTCCTTAC 
CATATGTTAG CAACCTGTGC AGAAGCCCTG CCCAGGCCCA ACTGGGAGCT GGCTCTGTAC 
ATCATCATCT CAGGAATAAT GAGTGCGCTG TTTCTCTTGG TAATTGGAAC AGCCTATTTA 
GAAGCTCAAG GAATTTGGGA GCCCTTCCGA AGGCGGCTGT CCTTTGAGGC CTCGAACCCG 
CCCTTTGATG TGGGGAGACC ATTTGATCTC AGGAGAATCG TTGGTATTTC ATCTGAAGGA 
AACTTGAACA CACTCAGCTG TGACCCCGGT CACAGTCGGG GCTTCTGTGG AGCAGGTGGC 
TCGGCCTCCC GGCCCGGCGC AGGGAGTCAC AAGCAGTGTA GCCCGTCGGT CCACCCGCAC 
AGCAGTCACA GCAATAGAAA CTCGGCTGAC GTGGAAAACG TCAGAGCCAA AAACAGTGCG 
AGTGCCTCTG GCAGGACTAA TGCCCAGTCG GCGAGCAAAG CCAGCCCACT GGTCTTCGAG 
TCCCACAGCA TGGCCCAGGG TCACACCGCA GGCCGGAAGT CCAAGGGGGC CAGACAGGGC 
CAGCACAGCA GCCAGCACCA TGGCCACAGC CCCCTGGAAC AGCACTCGCC CCCGCCGCCG 
CCGCCAGTGC CTCAGCAGCA GGAGCCACAG CCCGAGAGGC TGTCCCCTGC GCCCCTCGCA 
CCCCCGCCCC ACCCAGAGCA CCCCGGCAGC ACCCGGCACA GCTCCGAGGA CTCAGACATC 
ACCAGTCTCA TCGAAGCCAT GGACAAAGAC TTCGAGCACC ACGACGCCCC GCCCCTAGAT 
GTGTTTACAG AGCAGCCGCC TTCACCCTTG TCGAAAAGCA AAGGGAAAGG AAAGCCTCCT 
CAGCGCAAGG TGAAGCCCCC CAAGAAGCAG GAGGAGAAGG AGAAGAAGGG AAAGGGGAGG 
GCCCTGGAAG ACGAGCTGAA GGACTCCTTG GCTGACGACG ACAGCTCCTC CACCACCACA 
GAGACCTCCA ACCCAGACAC AGAGCCTCTC CTCAAGGAGG ATACAGAAAA GCAAAAGGGA 
AAACAAGCCA TGCCTGAAAA ACATGAAAAC GAAATGTCTC AAGTGAAACA AAAAAGCAAA 
AAAGTCTTAA ATGTTAAGAA AGAAATCCCA GCAGATGTGA AACCCAGTTG CTTAGAGCTA 
CCGTATACTG CCCCCTTGGA AAGCAAACAG CGCCGAAATC TCCCAACCAA GATTCCTCTT 
CCAACTACAC TGACCAGTGG ATCCAAACCA CGAAACTCCC AGAAAACAAA AGGTACAAGT 
AAGTTAGTGG ATAGCAGGCC ACCTGCCCTC GCAAAGTTTC TCCCAAACAG TCAAGAACTA 
GGCAACACCA GCAGCTCAGA AGGTGAAAAA GACTCTCCAC CACCAGAGTG GGATTCCGTG 
CCTGTTCACA AGCCCGGCAG CTCTACAGAT AGTCTTTATA AACTCTCTCT GCAAACCCTC 
AACGCAGACA TCTTCCTGAA ACAACGCCAG ACCTCGCCGC CGCCCACCTC CCCCTCACCG 
CCCGCCGCAC CCTGCCCGTT CACGTCCCGG GGCAGCTACA GCAGCATCGT CCATGGCAGC 
CCGGGCAGCG ACTCTAAAGC AAAGCAGCCA AGCGGGAGCA AACACAAACT GACAAAAGCC 
GCTTCTCTCC CGGGCAAGAA TGGCAACCCC ACTTTCGCTG CGGTCACGGC TGGGTATGAC 
AAGAGCCCAG GTGGGAACGG CTTTGCCAAA GTTTCTTCAA GCAAAACAGA TTTCTCTAGC 
AGCCTTGGCA TTTCACACAC TCCTGTTGAC AGCGATGGCT CAGACAGCTC GGGTCTGTGG 
AGCCCCGTGA GCAACCCCAG CAGCCCTGAC TTCACACCCC TCAACTCGTT CTCAGCCTTT 
GGGAATTCTT TTAACCTAAC TGGTGAAGTG TTCAGCAAGC TCGGGTTATC GCGATCATGC 
AATCAGCCCT CACAGAGGAG CTGGAATGAG CTGAGCAGCG GCCCCTCCTA CCTCTGGGAC 
TCGCCGGCCA CAGAGCCCAG CCCTTCCTGG CCAGCCAGTT CCAGCTCCCC GACCCACACG 
GCCACACCCA TCCTTGGCAG CACCAGCAGC CTGTGGTCGT CCACTCCGTT CAGCAGCTCC 
ATCTGGTCCA GCAGCCTGCA CAGCACCCTG CCCTTCACCA GCCCCACCAG CGCGTTGCCG 
AGCATCAGCC TCATGGGCCC CGAGAGCGCC CCGGCAGCTC ACGCGCCCTC TGCCGCCAGC 
CCCTCCGACG ACCTGGGCCA GACCTACAAC CCCTGGCGGA TATGGAGTCC CACCATCGGA 
CGGAGGAGCT CAGACCCCTG GTCGAATTCG CACTTCCCTC ACGAGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002691187.1
CDS	558..6188
Translation	MGKRAGGGAAGSAAAASTSAVAGLEPAAGRGGGPRSAAAGLLGALHLVMTLVVAAARAEKEAFIQSESIIEVLRFDDGGLLQTETTLGLSSYQQKSISLYRGNCRPIRFEPPMLDFHEQPVGMPKMEKVYLHNPSSEETITLVSISATTSHFHASFFQNRKILPGGNTSFDVVFLARVVGNVENTLFINTSNHGVFTYQVFGVGVPNPYRLRPFLGARVPVNSSFSPIINIHNPHSEPLQVVEMYSSGGDLHLELPTGQQGGTRKLWEIPPYETKGVMRASFSSREADNHTAFIRIKTNASDSTEFIILPVEVEVTTAPGIYSSTEMLDFGTLRTQDLPKVLNLHLLNSGTKDVPITSVRPTPQNDAVTVHFKPITLKASESKYTKVASISFDASKAKKPSQFSGKITVKAKEKSYSKLEIPYQAEVLDGYLGFDHAATLFHIRDSPADPVERPIYLTNTFSFAILIHDVLLPEEAKTMFKVHNFSKPVLILPNGSGYIFTLFFMPSTSSMHIDNNILLITNASKFHLPVRVYTGFLDYFVLPPKIEERFIDFGILSATEASNILFAIINSNPIELAIKSWHIIGDGLSIELVATERGNRTTIISSLPEFEKSSLSDQSSVILASGYFAVFRVKLTAKKLEGIHDGAIQITTDYEILTIPVKAVIAVGSLTCFPKHMVLPPSFPGKIVHQSLNIMNSFSQKVKIQQIRSLSEDVRFYYKRLRGNKEDLEPGKKSKIANIYFDPGLQCGDHCYVGLPFLSKSEPRVQPGIAMQEDAWDTDWDLHQSLFKGWMGIKENSGHKLSAMFEVNTDLQKNILSKVSAELSWPSLLSSPRLVRFPLTNTNCSSEEEIMLENPADVPIYIQFIPLALYSSPSIFVDKLISRFNLSKVAKIDLRTQEFQVFRNTAHPLQSSTGFTEGLSRHLVLNLILKPGEKKSVKVKFTPVHNRTVSSLIIIRNNLTVMDAVMVQGQGTTENLRVAGKLPGPGSSLRFKITEALLKDCTEGMRLREPNFTLKRTFKVENTGQLPIHIETIEVSGYSCEGYGFKVVNCQEFALSANASKDIVILFTPDFTASRVIRELKFITASGSEFVFVLNASLPYHMLATCAEALPRPNWELALYIIISGIMSALFLLVIGTAYLEAQGIWEPFRRRLSFEASNPPFDVGRPFDLRRIVGISSEGNLNTLSCDPGHSRGFCGAGGSASRPGAGSHKQCSPSVHPHSSHSNRNSADVENVRAKNSASASGRTNAQSASKASPLVFESHSMAQGHTAGRKSKGARQGQHSSQHHGHSPLEQHSPPPPPPVPQQQEPQPERLSPAPLAPPPHPEHPGSTRHSSEDSDITSLIEAMDKDFEHHDAPPLDVFTEQPPSPLSKSKGKGKPPQRKVKPPKKQEEKEKKGKGRALEDELKDSLADDDSSSTTTETSNPDTEPLLKEDTEKQKGKQAMPEKHENEMSQVKQKSKKVLNVKKEIPADVKPSCLELPYTAPLESKQRRNLPTKIPLPTTLTSGSKPRNSQKTKGTSKLVDSRPPALAKFLPNSQELGNTSSSEGEKDSPPPEWDSVPVHKPGSSTDSLYKLSLQTLNADIFLKQRQTSPPPTSPSPPAAPCPFTSRGSYSSIVHGSPGSDSKAKQPSGSKHKLTKAASLPGKNGNPTFAAVTAGYDKSPGGNGFAKVSSSKTDFSSSLGISHTPVDSDGSDSSGLWSPVSNPSSPDFTPLNSFSAFGNSFNLTGEVFSKLGLSRSCNQPSQRSWNELSSGPSYLWDSPATEPSPSWPASSSSPTHTATPILGSTSSLWSSTPFSSSIWSSSLHSTLPFTSPTSALPSISLMGPESAPAAHAPSAASPSDDLGQTYNPWRIWSPTIGRRSSDPWSNSHFPHEN

Target ORF information:

RefSeq Version	XM_002691141.5
Organism	Bos taurus(cattle)
Definition	Bos taurus transmembrane protein 131 (TMEM131), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002691141.5

ORF Insert Sequence:

ATGGGGAAGC GGGCCGGAGG AGGAGCAGCG GGATCCGCCG CCGCTGCCTC CACGTCCGCC 
GTGGCCGGTC TGGAGCCCGC GGCCGGCCGC GGCGGGGGCC CGCGGAGCGC AGCCGCCGGC 
CTCCTGGGAG CGCTGCACCT GGTGATGACC CTCGTCGTGG CCGCGGCGCG GGCCGAGAAG 
GAAGCATTTA TTCAGTCGGA GAGCATAATA GAAGTGCTGC GTTTTGATGA TGGAGGGCTT 
CTACAGACTG AGACAACACT TGGACTCAGT TCATACCAAC AGAAAAGTAT ATCTTTGTAC 
CGGGGTAATT GCAGACCTAT ACGGTTTGAG CCACCAATGC TGGATTTCCA TGAACAACCA 
GTTGGAATGC CAAAAATGGA AAAAGTTTAC TTACATAATC CTAGTTCTGA AGAAACTATT 
ACTTTAGTAT CAATATCTGC TACCACATCA CATTTTCACG CATCATTTTT TCAAAATAGG 
AAAATTCTTC CAGGAGGAAA TACATCATTT GATGTAGTTT TTCTTGCAAG AGTAGTGGGA 
AATGTAGAAA ATACTTTATT TATTAATACA TCTAATCATG GGGTATTCAC TTACCAGGTA 
TTTGGTGTTG GAGTTCCAAA TCCATACCGA TTGAGGCCAT TCCTTGGGGC CAGAGTCCCT 
GTGAATAGCA GTTTCTCACC CATAATAAAC ATACACAATC CTCACAGCGA GCCTTTACAG 
GTTGTAGAAA TGTACTCTAG TGGAGGAGAC CTTCACCTTG AACTTCCCAC AGGGCAGCAG 
GGAGGCACCA GGAAGCTGTG GGAAATTCCT CCTTATGAAA CCAAGGGAGT GATGAGAGCC 
AGTTTTTCAT CTAGAGAAGC AGATAATCAC ACAGCTTTCA TAAGAATAAA GACAAATGCT 
TCAGACAGCA CGGAGTTTAT CATCCTTCCG GTTGAAGTGG AAGTCACAAC AGCTCCTGGA 
ATTTATTCCT CAACTGAAAT GTTAGATTTT GGCACACTGC GAACACAAGA TCTACCAAAA 
GTTTTAAATC TTCATTTATT AAATTCAGGA ACAAAAGATG TACCAATAAC AAGTGTTCGA 
CCTACACCAC AAAATGATGC TGTCACTGTA CACTTTAAAC CAATTACATT AAAAGCTTCA 
GAAAGTAAAT ATACCAAGGT AGCAAGTATT AGTTTTGATG CATCAAAGGC GAAAAAACCA 
TCTCAGTTTT CTGGGAAAAT AACTGTGAAA GCAAAGGAAA AGAGTTATTC TAAACTTGAA 
ATACCATATC AAGCAGAGGT TTTAGATGGT TATTTGGGAT TTGATCACGC TGCAACATTA 
TTTCACATTC GAGACAGTCC TGCTGATCCT GTGGAAAGGC CAATTTACCT TACTAACACT 
TTCAGCTTTG CGATCCTCAT TCATGATGTG TTGCTACCAG AAGAAGCCAA AACAATGTTT 
AAAGTTCACA ACTTCAGCAA ACCAGTCTTA ATTCTACCAA ATGGATCAGG CTACATTTTT 
ACTCTATTTT TTATGCCATC CACATCGTCC ATGCACATAG ATAACAATAT TTTACTTATT 
ACCAATGCCT CTAAGTTTCA CTTACCTGTG CGGGTATATA CAGGCTTTTT AGATTACTTT 
GTATTACCCC CCAAAATAGA AGAACGTTTC ATAGATTTTG GTATACTGAG TGCAACAGAA 
GCAAGTAATA TTTTATTTGC CATTATAAAC AGCAATCCAA TTGAGTTGGC TATAAAAAGC 
TGGCATATTA TAGGAGATGG TTTATCAATA GAACTCGTAG CTACTGAAAG AGGCAATAGA 
ACTACAATCA TCTCAAGCCT TCCAGAGTTT GAAAAATCCT CTCTATCAGA CCAGTCATCG 
GTAATACTAG CTTCAGGATA CTTTGCAGTG TTCAGAGTCA AACTTACTGC AAAAAAACTG 
GAAGGAATTC ATGATGGGGC TATACAGATC ACGACAGACT ATGAGATCCT AACAATTCCT 
GTGAAGGCTG TGATCGCAGT GGGTTCTTTG ACTTGCTTCC CAAAGCACAT GGTTCTTCCA 
CCTTCCTTTC CAGGGAAAAT AGTTCATCAG AGTTTAAATA TTATGAATTC CTTCTCACAG 
AAGGTAAAGA TCCAACAGAT ACGATCTTTG TCAGAAGACG TGCGTTTTTA CTACAAACGA 
TTGCGGGGTA ATAAGGAAGA CTTGGAGCCA GGGAAAAAAT CAAAGATTGC AAACATTTAC 
TTTGATCCTG GATTGCAGTG CGGGGATCAT TGCTATGTCG GCTTGCCTTT TCTGTCCAAA 
TCTGAACCCA GAGTCCAGCC TGGCATAGCC ATGCAGGAAG ATGCCTGGGA CACCGACTGG 
GATTTGCATC AAAGCCTATT CAAGGGATGG ATGGGAATAA AGGAAAACTC AGGTCACAAA 
TTGAGCGCTA TGTTTGAAGT GAATACAGAC CTTCAGAAAA ACATCCTGTC AAAAGTTTCT 
GCCGAGCTCT CCTGGCCATC CCTCCTCAGC TCCCCCCGGC TCGTGAGGTT TCCCCTCACT 
AATACAAACT GCTCTTCAGA AGAAGAGATT ATGTTAGAAA ACCCTGCAGA TGTTCCCATC 
TATATTCAAT TTATCCCCCT GGCTTTATAT TCCAGCCCTT CCATCTTTGT AGATAAGTTA 
ATATCAAGGT TTAACTTAAG TAAGGTGGCA AAGATCGATT TAAGAACACA AGAATTCCAA 
GTCTTTAGAA ACACTGCTCA CCCACTGCAG AGTTCAACAG GATTCACAGA GGGCCTCTCC 
CGACATTTAG TTTTAAACTT AATTTTAAAA CCTGGAGAAA AGAAATCTGT CAAAGTAAAG 
TTTACTCCAG TTCACAATAG AACTGTTTCT TCCCTAATCA TAATCAGAAA TAACCTGACT 
GTGATGGATG CCGTAATGGT CCAAGGACAG GGGACAACCG AGAACCTGAG GGTGGCAGGC 
AAGCTTCCGG GCCCAGGGAG CTCCTTACGC TTCAAAATCA CAGAAGCACT GCTGAAAGAC 
TGTACGGAGG GTATGAGACT AAGGGAACCC AATTTCACAT TGAAAAGAAC ATTTAAAGTA 
GAGAACACAG GACAACTTCC AATTCACATA GAAACCATTG AAGTCAGTGG GTACTCTTGT 
GAGGGATATG GCTTTAAAGT CGTCAATTGT CAAGAGTTTG CACTGAGCGC CAATGCCTCT 
AAAGACATAG TCATTCTGTT TACTCCTGAT TTCACGGCTT CTAGAGTCAT CCGTGAACTG 
AAGTTTATAA CAGCCAGTGG CTCTGAGTTT GTGTTTGTAT TGAATGCATC CCTTCCTTAC 
CATATGTTAG CAACCTGTGC AGAAGCCCTG CCCAGGCCCA ACTGGGAGCT GGCTCTGTAC 
ATCATCATCT CAGGAATAAT GAGTGCGCTG TTTCTCTTGG TAATTGGAAC AGCCTATTTA 
GAAGCTCAAG GAATTTGGGA GCCCTTCCGA AGGCGGCTGT CCTTTGAGGC CTCGAACCCG 
CCCTTTGATG TGGGGAGACC ATTTGATCTC AGGAGAATCG TTGGTATTTC ATCTGAAGGA 
AACTTGAACA CACTCAGCTG TGACCCCGGT CACAGTCGGG GCTTCTGTGG AGCAGGTGGC 
TCGGCCTCCC GGCCCGGCGC AGGGAGTCAC AAGCAGTGTA GCCCGTCGGT CCACCCGCAC 
AGCAGTCACA GCAATAGAAA CTCGGCTGAC GTGGAAAACG TCAGAGCCAA AAACAGTGCG 
AGTGCCTCTG GCAGGACTAA TGCCCAGTCG GCGAGCAAAG CCAGCCCACT GGTCTTCGAG 
TCCCACAGCA TGGCCCAGGG TCACACCGCA GGCCGGAAGT CCAAGGGGGC CAGACAGGGC 
CAGCACAGCA GCCAGCACCA TGGCCACAGC CCCCTGGAAC AGCACTCGCC CCCGCCGCCG 
CCGCCAGTGC CTCAGCAGCA GGAGCCACAG CCCGAGAGGC TGTCCCCTGC GCCCCTCGCA 
CCCCCGCCCC ACCCAGAGCA CCCCGGCAGC ACCCGGCACA GCTCCGAGGA CTCAGACATC 
ACCAGTCTCA TCGAAGCCAT GGACAAAGAC TTCGAGCACC ACGACGCCCC GCCCCTAGAT 
GTGTTTACAG AGCAGCCGCC TTCACCCTTG TCGAAAAGCA AAGGGAAAGG AAAGCCTCCT 
CAGCGCAAGG TGAAGCCCCC CAAGAAGCAG GAGGAGAAGG AGAAGAAGGG AAAGGGGAGG 
GCCCTGGAAG ACGAGCTGAA GGACTCCTTG GCTGACGACG ACAGCTCCTC CACCACCACA 
GAGACCTCCA ACCCAGACAC AGAGCCTCTC CTCAAGGAGG ATACAGAAAA GCAAAAGGGA 
AAACAAGCCA TGCCTGAAAA ACATGAAAAC GAAATGTCTC AAGTGAAACA AAAAAGCAAA 
AAAGTCTTAA ATGTTAAGAA AGAAATCCCA GCAGATGTGA AACCCAGTTG CTTAGAGCTA 
CCGTATACTG CCCCCTTGGA AAGCAAACAG CGCCGAAATC TCCCAACCAA GATTCCTCTT 
CCAACTACAC TGACCAGTGG ATCCAAACCA CGAAACTCCC AGAAAACAAA AGGTACAAGT 
AAGTTAGTGG ATAGCAGGCC ACCTGCCCTC GCAAAGTTTC TCCCAAACAG TCAAGAACTA 
GGCAACACCA GCAGCTCAGA AGGTGAAAAA GACTCTCCAC CACCAGAGTG GGATTCCGTG 
CCTGTTCACA AGCCCGGCAG CTCTACAGAT AGTCTTTATA AACTCTCTCT GCAAACCCTC 
AACGCAGACA TCTTCCTGAA ACAACGCCAG ACCTCGCCGC CGCCCACCTC CCCCTCACCG 
CCCGCCGCAC CCTGCCCGTT CACGTCCCGG GGCAGCTACA GCAGCATCGT CCATGGCAGC 
CCGGGCAGCG ACTCTAAAGC AAAGCAGCCA AGCGGGAGCA AACACAAACT GACAAAAGCC 
GCTTCTCTCC CGGGCAAGAA TGGCAACCCC ACTTTCGCTG CGGTCACGGC TGGGTATGAC 
AAGAGCCCAG GTGGGAACGG CTTTGCCAAA GTTTCTTCAA GCAAAACAGA TTTCTCTAGC 
AGCCTTGGCA TTTCACACAC TCCTGTTGAC AGCGATGGCT CAGACAGCTC GGGTCTGTGG 
AGCCCCGTGA GCAACCCCAG CAGCCCTGAC TTCACACCCC TCAACTCGTT CTCAGCCTTT 
GGGAATTCTT TTAACCTAAC TGGTGAAGTG TTCAGCAAGC TCGGGTTATC GCGATCATGC 
AATCAGCCCT CACAGAGGAG CTGGAATGAG CTGAGCAGCG GCCCCTCCTA CCTCTGGGAC 
TCGCCGGCCA CAGAGCCCAG CCCTTCCTGG CCAGCCAGTT CCAGCTCCCC GACCCACACG 
GCCACACCCA TCCTTGGCAG CACCAGCAGC CTGTGGTCGT CCACTCCGTT CAGCAGCTCC 
ATCTGGTCCA GCAGCCTGCA CAGCACCCTG CCCTTCACCA GCCCCACCAG CGCGTTGCCG 
AGCATCAGCC TCATGGGCCC CGAGAGCGCC CCGGCAGCTC ACGCGCCCTC TGCCGCCAGC 
CCCTCCGACG ACCTGGGCCA GACCTACAAC CCCTGGCGGA TATGGAGTCC CACCATCGGA 
CGGAGGAGCT CAGACCCCTG GTCGAATTCG CACTTCCCTC ACGAGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM131 ( XM_002691141.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002691187.1 Bos taurus transmembrane protein 131 (TMEM131), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa06771
Clone ID Related Accession (Same CDS sequence)	XM_002691141.5 , XM_002691141.6 , XM_613474.9
Accession Version	XM_002691141.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transmembrane protein 131 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002691141.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGC GGGCCGGAGG AGGAGCAGCG GGATCCGCCG CCGCTGCCTC CACGTCCGCC 
GTGGCCGGTC TGGAGCCCGC GGCCGGCCGC GGCGGGGGCC CGCGGAGCGC AGCCGCCGGC 
CTCCTGGGAG CGCTGCACCT GGTGATGACC CTCGTCGTGG CCGCGGCGCG GGCCGAGAAG 
GAAGCATTTA TTCAGTCGGA GAGCATAATA GAAGTGCTGC GTTTTGATGA TGGAGGGCTT 
CTACAGACTG AGACAACACT TGGACTCAGT TCATACCAAC AGAAAAGTAT ATCTTTGTAC 
CGGGGTAATT GCAGACCTAT ACGGTTTGAG CCACCAATGC TGGATTTCCA TGAACAACCA 
GTTGGAATGC CAAAAATGGA AAAAGTTTAC TTACATAATC CTAGTTCTGA AGAAACTATT 
ACTTTAGTAT CAATATCTGC TACCACATCA CATTTTCACG CATCATTTTT TCAAAATAGG 
AAAATTCTTC CAGGAGGAAA TACATCATTT GATGTAGTTT TTCTTGCAAG AGTAGTGGGA 
AATGTAGAAA ATACTTTATT TATTAATACA TCTAATCATG GGGTATTCAC TTACCAGGTA 
TTTGGTGTTG GAGTTCCAAA TCCATACCGA TTGAGGCCAT TCCTTGGGGC CAGAGTCCCT 
GTGAATAGCA GTTTCTCACC CATAATAAAC ATACACAATC CTCACAGCGA GCCTTTACAG 
GTTGTAGAAA TGTACTCTAG TGGAGGAGAC CTTCACCTTG AACTTCCCAC AGGGCAGCAG 
GGAGGCACCA GGAAGCTGTG GGAAATTCCT CCTTATGAAA CCAAGGGAGT GATGAGAGCC 
AGTTTTTCAT CTAGAGAAGC AGATAATCAC ACAGCTTTCA TAAGAATAAA GACAAATGCT 
TCAGACAGCA CGGAGTTTAT CATCCTTCCG GTTGAAGTGG AAGTCACAAC AGCTCCTGGA 
ATTTATTCCT CAACTGAAAT GTTAGATTTT GGCACACTGC GAACACAAGA TCTACCAAAA 
GTTTTAAATC TTCATTTATT AAATTCAGGA ACAAAAGATG TACCAATAAC AAGTGTTCGA 
CCTACACCAC AAAATGATGC TGTCACTGTA CACTTTAAAC CAATTACATT AAAAGCTTCA 
GAAAGTAAAT ATACCAAGGT AGCAAGTATT AGTTTTGATG CATCAAAGGC GAAAAAACCA 
TCTCAGTTTT CTGGGAAAAT AACTGTGAAA GCAAAGGAAA AGAGTTATTC TAAACTTGAA 
ATACCATATC AAGCAGAGGT TTTAGATGGT TATTTGGGAT TTGATCACGC TGCAACATTA 
TTTCACATTC GAGACAGTCC TGCTGATCCT GTGGAAAGGC CAATTTACCT TACTAACACT 
TTCAGCTTTG CGATCCTCAT TCATGATGTG TTGCTACCAG AAGAAGCCAA AACAATGTTT 
AAAGTTCACA ACTTCAGCAA ACCAGTCTTA ATTCTACCAA ATGGATCAGG CTACATTTTT 
ACTCTATTTT TTATGCCATC CACATCGTCC ATGCACATAG ATAACAATAT TTTACTTATT 
ACCAATGCCT CTAAGTTTCA CTTACCTGTG CGGGTATATA CAGGCTTTTT AGATTACTTT 
GTATTACCCC CCAAAATAGA AGAACGTTTC ATAGATTTTG GTATACTGAG TGCAACAGAA 
GCAAGTAATA TTTTATTTGC CATTATAAAC AGCAATCCAA TTGAGTTGGC TATAAAAAGC 
TGGCATATTA TAGGAGATGG TTTATCAATA GAACTCGTAG CTACTGAAAG AGGCAATAGA 
ACTACAATCA TCTCAAGCCT TCCAGAGTTT GAAAAATCCT CTCTATCAGA CCAGTCATCG 
GTAATACTAG CTTCAGGATA CTTTGCAGTG TTCAGAGTCA AACTTACTGC AAAAAAACTG 
GAAGGAATTC ATGATGGGGC TATACAGATC ACGACAGACT ATGAGATCCT AACAATTCCT 
GTGAAGGCTG TGATCGCAGT GGGTTCTTTG ACTTGCTTCC CAAAGCACAT GGTTCTTCCA 
CCTTCCTTTC CAGGGAAAAT AGTTCATCAG AGTTTAAATA TTATGAATTC CTTCTCACAG 
AAGGTAAAGA TCCAACAGAT ACGATCTTTG TCAGAAGACG TGCGTTTTTA CTACAAACGA 
TTGCGGGGTA ATAAGGAAGA CTTGGAGCCA GGGAAAAAAT CAAAGATTGC AAACATTTAC 
TTTGATCCTG GATTGCAGTG CGGGGATCAT TGCTATGTCG GCTTGCCTTT TCTGTCCAAA 
TCTGAACCCA GAGTCCAGCC TGGCATAGCC ATGCAGGAAG ATGCCTGGGA CACCGACTGG 
GATTTGCATC AAAGCCTATT CAAGGGATGG ATGGGAATAA AGGAAAACTC AGGTCACAAA 
TTGAGCGCTA TGTTTGAAGT GAATACAGAC CTTCAGAAAA ACATCCTGTC AAAAGTTTCT 
GCCGAGCTCT CCTGGCCATC CCTCCTCAGC TCCCCCCGGC TCGTGAGGTT TCCCCTCACT 
AATACAAACT GCTCTTCAGA AGAAGAGATT ATGTTAGAAA ACCCTGCAGA TGTTCCCATC 
TATATTCAAT TTATCCCCCT GGCTTTATAT TCCAGCCCTT CCATCTTTGT AGATAAGTTA 
ATATCAAGGT TTAACTTAAG TAAGGTGGCA AAGATCGATT TAAGAACACA AGAATTCCAA 
GTCTTTAGAA ACACTGCTCA CCCACTGCAG AGTTCAACAG GATTCACAGA GGGCCTCTCC 
CGACATTTAG TTTTAAACTT AATTTTAAAA CCTGGAGAAA AGAAATCTGT CAAAGTAAAG 
TTTACTCCAG TTCACAATAG AACTGTTTCT TCCCTAATCA TAATCAGAAA TAACCTGACT 
GTGATGGATG CCGTAATGGT CCAAGGACAG GGGACAACCG AGAACCTGAG GGTGGCAGGC 
AAGCTTCCGG GCCCAGGGAG CTCCTTACGC TTCAAAATCA CAGAAGCACT GCTGAAAGAC 
TGTACGGAGG GTATGAGACT AAGGGAACCC AATTTCACAT TGAAAAGAAC ATTTAAAGTA 
GAGAACACAG GACAACTTCC AATTCACATA GAAACCATTG AAGTCAGTGG GTACTCTTGT 
GAGGGATATG GCTTTAAAGT CGTCAATTGT CAAGAGTTTG CACTGAGCGC CAATGCCTCT 
AAAGACATAG TCATTCTGTT TACTCCTGAT TTCACGGCTT CTAGAGTCAT CCGTGAACTG 
AAGTTTATAA CAGCCAGTGG CTCTGAGTTT GTGTTTGTAT TGAATGCATC CCTTCCTTAC 
CATATGTTAG CAACCTGTGC AGAAGCCCTG CCCAGGCCCA ACTGGGAGCT GGCTCTGTAC 
ATCATCATCT CAGGAATAAT GAGTGCGCTG TTTCTCTTGG TAATTGGAAC AGCCTATTTA 
GAAGCTCAAG GAATTTGGGA GCCCTTCCGA AGGCGGCTGT CCTTTGAGGC CTCGAACCCG 
CCCTTTGATG TGGGGAGACC ATTTGATCTC AGGAGAATCG TTGGTATTTC ATCTGAAGGA 
AACTTGAACA CACTCAGCTG TGACCCCGGT CACAGTCGGG GCTTCTGTGG AGCAGGTGGC 
TCGGCCTCCC GGCCCGGCGC AGGGAGTCAC AAGCAGTGTA GCCCGTCGGT CCACCCGCAC 
AGCAGTCACA GCAATAGAAA CTCGGCTGAC GTGGAAAACG TCAGAGCCAA AAACAGTGCG 
AGTGCCTCTG GCAGGACTAA TGCCCAGTCG GCGAGCAAAG CCAGCCCACT GGTCTTCGAG 
TCCCACAGCA TGGCCCAGGG TCACACCGCA GGCCGGAAGT CCAAGGGGGC CAGACAGGGC 
CAGCACAGCA GCCAGCACCA TGGCCACAGC CCCCTGGAAC AGCACTCGCC CCCGCCGCCG 
CCGCCAGTGC CTCAGCAGCA GGAGCCACAG CCCGAGAGGC TGTCCCCTGC GCCCCTCGCA 
CCCCCGCCCC ACCCAGAGCA CCCCGGCAGC ACCCGGCACA GCTCCGAGGA CTCAGACATC 
ACCAGTCTCA TCGAAGCCAT GGACAAAGAC TTCGAGCACC ACGACGCCCC GCCCCTAGAT 
GTGTTTACAG AGCAGCCGCC TTCACCCTTG TCGAAAAGCA AAGGGAAAGG AAAGCCTCCT 
CAGCGCAAGG TGAAGCCCCC CAAGAAGCAG GAGGAGAAGG AGAAGAAGGG AAAGGGGAGG 
GCCCTGGAAG ACGAGCTGAA GGACTCCTTG GCTGACGACG ACAGCTCCTC CACCACCACA 
GAGACCTCCA ACCCAGACAC AGAGCCTCTC CTCAAGGAGG ATACAGAAAA GCAAAAGGGA 
AAACAAGCCA TGCCTGAAAA ACATGAAAAC GAAATGTCTC AAGTGAAACA AAAAAGCAAA 
AAAGTCTTAA ATGTTAAGAA AGAAATCCCA GCAGATGTGA AACCCAGTTG CTTAGAGCTA 
CCGTATACTG CCCCCTTGGA AAGCAAACAG CGCCGAAATC TCCCAACCAA GATTCCTCTT 
CCAACTACAC TGACCAGTGG ATCCAAACCA CGAAACTCCC AGAAAACAAA AGGTACAAGT 
AAGTTAGTGG ATAGCAGGCC ACCTGCCCTC GCAAAGTTTC TCCCAAACAG TCAAGAACTA 
GGCAACACCA GCAGCTCAGA AGGTGAAAAA GACTCTCCAC CACCAGAGTG GGATTCCGTG 
CCTGTTCACA AGCCCGGCAG CTCTACAGAT AGTCTTTATA AACTCTCTCT GCAAACCCTC 
AACGCAGACA TCTTCCTGAA ACAACGCCAG ACCTCGCCGC CGCCCACCTC CCCCTCACCG 
CCCGCCGCAC CCTGCCCGTT CACGTCCCGG GGCAGCTACA GCAGCATCGT CCATGGCAGC 
CCGGGCAGCG ACTCTAAAGC AAAGCAGCCA AGCGGGAGCA AACACAAACT GACAAAAGCC 
GCTTCTCTCC CGGGCAAGAA TGGCAACCCC ACTTTCGCTG CGGTCACGGC TGGGTATGAC 
AAGAGCCCAG GTGGGAACGG CTTTGCCAAA GTTTCTTCAA GCAAAACAGA TTTCTCTAGC 
AGCCTTGGCA TTTCACACAC TCCTGTTGAC AGCGATGGCT CAGACAGCTC GGGTCTGTGG 
AGCCCCGTGA GCAACCCCAG CAGCCCTGAC TTCACACCCC TCAACTCGTT CTCAGCCTTT 
GGGAATTCTT TTAACCTAAC TGGTGAAGTG TTCAGCAAGC TCGGGTTATC GCGATCATGC 
AATCAGCCCT CACAGAGGAG CTGGAATGAG CTGAGCAGCG GCCCCTCCTA CCTCTGGGAC 
TCGCCGGCCA CAGAGCCCAG CCCTTCCTGG CCAGCCAGTT CCAGCTCCCC GACCCACACG 
GCCACACCCA TCCTTGGCAG CACCAGCAGC CTGTGGTCGT CCACTCCGTT CAGCAGCTCC 
ATCTGGTCCA GCAGCCTGCA CAGCACCCTG CCCTTCACCA GCCCCACCAG CGCGTTGCCG 
AGCATCAGCC TCATGGGCCC CGAGAGCGCC CCGGCAGCTC ACGCGCCCTC TGCCGCCAGC 
CCCTCCGACG ACCTGGGCCA GACCTACAAC CCCTGGCGGA TATGGAGTCC CACCATCGGA 
CGGAGGAGCT CAGACCCCTG GTCGAATTCG CACTTCCCTC ACGAGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002691187.1
CDS	558..6188
Translation	MGKRAGGGAAGSAAAASTSAVAGLEPAAGRGGGPRSAAAGLLGALHLVMTLVVAAARAEKEAFIQSESIIEVLRFDDGGLLQTETTLGLSSYQQKSISLYRGNCRPIRFEPPMLDFHEQPVGMPKMEKVYLHNPSSEETITLVSISATTSHFHASFFQNRKILPGGNTSFDVVFLARVVGNVENTLFINTSNHGVFTYQVFGVGVPNPYRLRPFLGARVPVNSSFSPIINIHNPHSEPLQVVEMYSSGGDLHLELPTGQQGGTRKLWEIPPYETKGVMRASFSSREADNHTAFIRIKTNASDSTEFIILPVEVEVTTAPGIYSSTEMLDFGTLRTQDLPKVLNLHLLNSGTKDVPITSVRPTPQNDAVTVHFKPITLKASESKYTKVASISFDASKAKKPSQFSGKITVKAKEKSYSKLEIPYQAEVLDGYLGFDHAATLFHIRDSPADPVERPIYLTNTFSFAILIHDVLLPEEAKTMFKVHNFSKPVLILPNGSGYIFTLFFMPSTSSMHIDNNILLITNASKFHLPVRVYTGFLDYFVLPPKIEERFIDFGILSATEASNILFAIINSNPIELAIKSWHIIGDGLSIELVATERGNRTTIISSLPEFEKSSLSDQSSVILASGYFAVFRVKLTAKKLEGIHDGAIQITTDYEILTIPVKAVIAVGSLTCFPKHMVLPPSFPGKIVHQSLNIMNSFSQKVKIQQIRSLSEDVRFYYKRLRGNKEDLEPGKKSKIANIYFDPGLQCGDHCYVGLPFLSKSEPRVQPGIAMQEDAWDTDWDLHQSLFKGWMGIKENSGHKLSAMFEVNTDLQKNILSKVSAELSWPSLLSSPRLVRFPLTNTNCSSEEEIMLENPADVPIYIQFIPLALYSSPSIFVDKLISRFNLSKVAKIDLRTQEFQVFRNTAHPLQSSTGFTEGLSRHLVLNLILKPGEKKSVKVKFTPVHNRTVSSLIIIRNNLTVMDAVMVQGQGTTENLRVAGKLPGPGSSLRFKITEALLKDCTEGMRLREPNFTLKRTFKVENTGQLPIHIETIEVSGYSCEGYGFKVVNCQEFALSANASKDIVILFTPDFTASRVIRELKFITASGSEFVFVLNASLPYHMLATCAEALPRPNWELALYIIISGIMSALFLLVIGTAYLEAQGIWEPFRRRLSFEASNPPFDVGRPFDLRRIVGISSEGNLNTLSCDPGHSRGFCGAGGSASRPGAGSHKQCSPSVHPHSSHSNRNSADVENVRAKNSASASGRTNAQSASKASPLVFESHSMAQGHTAGRKSKGARQGQHSSQHHGHSPLEQHSPPPPPPVPQQQEPQPERLSPAPLAPPPHPEHPGSTRHSSEDSDITSLIEAMDKDFEHHDAPPLDVFTEQPPSPLSKSKGKGKPPQRKVKPPKKQEEKEKKGKGRALEDELKDSLADDDSSSTTTETSNPDTEPLLKEDTEKQKGKQAMPEKHENEMSQVKQKSKKVLNVKKEIPADVKPSCLELPYTAPLESKQRRNLPTKIPLPTTLTSGSKPRNSQKTKGTSKLVDSRPPALAKFLPNSQELGNTSSSEGEKDSPPPEWDSVPVHKPGSSTDSLYKLSLQTLNADIFLKQRQTSPPPTSPSPPAAPCPFTSRGSYSSIVHGSPGSDSKAKQPSGSKHKLTKAASLPGKNGNPTFAAVTAGYDKSPGGNGFAKVSSSKTDFSSSLGISHTPVDSDGSDSSGLWSPVSNPSSPDFTPLNSFSAFGNSFNLTGEVFSKLGLSRSCNQPSQRSWNELSSGPSYLWDSPATEPSPSWPASSSSPTHTATPILGSTSSLWSSTPFSSSIWSSSLHSTLPFTSPTSALPSISLMGPESAPAAHAPSAASPSDDLGQTYNPWRIWSPTIGRRSSDPWSNSHFPHEN

Target ORF information:

RefSeq Version	XM_002691141.6
Organism	Bos taurus(cattle)
Definition	Bos taurus transmembrane protein 131 (TMEM131), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002691141.6

ORF Insert Sequence:

ATGGGGAAGC GGGCCGGAGG AGGAGCAGCG GGATCCGCCG CCGCTGCCTC CACGTCCGCC 
GTGGCCGGTC TGGAGCCCGC GGCCGGCCGC GGCGGGGGCC CGCGGAGCGC AGCCGCCGGC 
CTCCTGGGAG CGCTGCACCT GGTGATGACC CTCGTCGTGG CCGCGGCGCG GGCCGAGAAG 
GAAGCATTTA TTCAGTCGGA GAGCATAATA GAAGTGCTGC GTTTTGATGA TGGAGGGCTT 
CTACAGACTG AGACAACACT TGGACTCAGT TCATACCAAC AGAAAAGTAT ATCTTTGTAC 
CGGGGTAATT GCAGACCTAT ACGGTTTGAG CCACCAATGC TGGATTTCCA TGAACAACCA 
GTTGGAATGC CAAAAATGGA AAAAGTTTAC TTACATAATC CTAGTTCTGA AGAAACTATT 
ACTTTAGTAT CAATATCTGC TACCACATCA CATTTTCACG CATCATTTTT TCAAAATAGG 
AAAATTCTTC CAGGAGGAAA TACATCATTT GATGTAGTTT TTCTTGCAAG AGTAGTGGGA 
AATGTAGAAA ATACTTTATT TATTAATACA TCTAATCATG GGGTATTCAC TTACCAGGTA 
TTTGGTGTTG GAGTTCCAAA TCCATACCGA TTGAGGCCAT TCCTTGGGGC CAGAGTCCCT 
GTGAATAGCA GTTTCTCACC CATAATAAAC ATACACAATC CTCACAGCGA GCCTTTACAG 
GTTGTAGAAA TGTACTCTAG TGGAGGAGAC CTTCACCTTG AACTTCCCAC AGGGCAGCAG 
GGAGGCACCA GGAAGCTGTG GGAAATTCCT CCTTATGAAA CCAAGGGAGT GATGAGAGCC 
AGTTTTTCAT CTAGAGAAGC AGATAATCAC ACAGCTTTCA TAAGAATAAA GACAAATGCT 
TCAGACAGCA CGGAGTTTAT CATCCTTCCG GTTGAAGTGG AAGTCACAAC AGCTCCTGGA 
ATTTATTCCT CAACTGAAAT GTTAGATTTT GGCACACTGC GAACACAAGA TCTACCAAAA 
GTTTTAAATC TTCATTTATT AAATTCAGGA ACAAAAGATG TACCAATAAC AAGTGTTCGA 
CCTACACCAC AAAATGATGC TGTCACTGTA CACTTTAAAC CAATTACATT AAAAGCTTCA 
GAAAGTAAAT ATACCAAGGT AGCAAGTATT AGTTTTGATG CATCAAAGGC GAAAAAACCA 
TCTCAGTTTT CTGGGAAAAT AACTGTGAAA GCAAAGGAAA AGAGTTATTC TAAACTTGAA 
ATACCATATC AAGCAGAGGT TTTAGATGGT TATTTGGGAT TTGATCACGC TGCAACATTA 
TTTCACATTC GAGACAGTCC TGCTGATCCT GTGGAAAGGC CAATTTACCT TACTAACACT 
TTCAGCTTTG CGATCCTCAT TCATGATGTG TTGCTACCAG AAGAAGCCAA AACAATGTTT 
AAAGTTCACA ACTTCAGCAA ACCAGTCTTA ATTCTACCAA ATGGATCAGG CTACATTTTT 
ACTCTATTTT TTATGCCATC CACATCGTCC ATGCACATAG ATAACAATAT TTTACTTATT 
ACCAATGCCT CTAAGTTTCA CTTACCTGTG CGGGTATATA CAGGCTTTTT AGATTACTTT 
GTATTACCCC CCAAAATAGA AGAACGTTTC ATAGATTTTG GTATACTGAG TGCAACAGAA 
GCAAGTAATA TTTTATTTGC CATTATAAAC AGCAATCCAA TTGAGTTGGC TATAAAAAGC 
TGGCATATTA TAGGAGATGG TTTATCAATA GAACTCGTAG CTACTGAAAG AGGCAATAGA 
ACTACAATCA TCTCAAGCCT TCCAGAGTTT GAAAAATCCT CTCTATCAGA CCAGTCATCG 
GTAATACTAG CTTCAGGATA CTTTGCAGTG TTCAGAGTCA AACTTACTGC AAAAAAACTG 
GAAGGAATTC ATGATGGGGC TATACAGATC ACGACAGACT ATGAGATCCT AACAATTCCT 
GTGAAGGCTG TGATCGCAGT GGGTTCTTTG ACTTGCTTCC CAAAGCACAT GGTTCTTCCA 
CCTTCCTTTC CAGGGAAAAT AGTTCATCAG AGTTTAAATA TTATGAATTC CTTCTCACAG 
AAGGTAAAGA TCCAACAGAT ACGATCTTTG TCAGAAGACG TGCGTTTTTA CTACAAACGA 
TTGCGGGGTA ATAAGGAAGA CTTGGAGCCA GGGAAAAAAT CAAAGATTGC AAACATTTAC 
TTTGATCCTG GATTGCAGTG CGGGGATCAT TGCTATGTCG GCTTGCCTTT TCTGTCCAAA 
TCTGAACCCA GAGTCCAGCC TGGCATAGCC ATGCAGGAAG ATGCCTGGGA CACCGACTGG 
GATTTGCATC AAAGCCTATT CAAGGGATGG ATGGGAATAA AGGAAAACTC AGGTCACAAA 
TTGAGCGCTA TGTTTGAAGT GAATACAGAC CTTCAGAAAA ACATCCTGTC AAAAGTTTCT 
GCCGAGCTCT CCTGGCCATC CCTCCTCAGC TCCCCCCGGC TCGTGAGGTT TCCCCTCACT 
AATACAAACT GCTCTTCAGA AGAAGAGATT ATGTTAGAAA ACCCTGCAGA TGTTCCCATC 
TATATTCAAT TTATCCCCCT GGCTTTATAT TCCAGCCCTT CCATCTTTGT AGATAAGTTA 
ATATCAAGGT TTAACTTAAG TAAGGTGGCA AAGATCGATT TAAGAACACA AGAATTCCAA 
GTCTTTAGAA ACACTGCTCA CCCACTGCAG AGTTCAACAG GATTCACAGA GGGCCTCTCC 
CGACATTTAG TTTTAAACTT AATTTTAAAA CCTGGAGAAA AGAAATCTGT CAAAGTAAAG 
TTTACTCCAG TTCACAATAG AACTGTTTCT TCCCTAATCA TAATCAGAAA TAACCTGACT 
GTGATGGATG CCGTAATGGT CCAAGGACAG GGGACAACCG AGAACCTGAG GGTGGCAGGC 
AAGCTTCCGG GCCCAGGGAG CTCCTTACGC TTCAAAATCA CAGAAGCACT GCTGAAAGAC 
TGTACGGAGG GTATGAGACT AAGGGAACCC AATTTCACAT TGAAAAGAAC ATTTAAAGTA 
GAGAACACAG GACAACTTCC AATTCACATA GAAACCATTG AAGTCAGTGG GTACTCTTGT 
GAGGGATATG GCTTTAAAGT CGTCAATTGT CAAGAGTTTG CACTGAGCGC CAATGCCTCT 
AAAGACATAG TCATTCTGTT TACTCCTGAT TTCACGGCTT CTAGAGTCAT CCGTGAACTG 
AAGTTTATAA CAGCCAGTGG CTCTGAGTTT GTGTTTGTAT TGAATGCATC CCTTCCTTAC 
CATATGTTAG CAACCTGTGC AGAAGCCCTG CCCAGGCCCA ACTGGGAGCT GGCTCTGTAC 
ATCATCATCT CAGGAATAAT GAGTGCGCTG TTTCTCTTGG TAATTGGAAC AGCCTATTTA 
GAAGCTCAAG GAATTTGGGA GCCCTTCCGA AGGCGGCTGT CCTTTGAGGC CTCGAACCCG 
CCCTTTGATG TGGGGAGACC ATTTGATCTC AGGAGAATCG TTGGTATTTC ATCTGAAGGA 
AACTTGAACA CACTCAGCTG TGACCCCGGT CACAGTCGGG GCTTCTGTGG AGCAGGTGGC 
TCGGCCTCCC GGCCCGGCGC AGGGAGTCAC AAGCAGTGTA GCCCGTCGGT CCACCCGCAC 
AGCAGTCACA GCAATAGAAA CTCGGCTGAC GTGGAAAACG TCAGAGCCAA AAACAGTGCG 
AGTGCCTCTG GCAGGACTAA TGCCCAGTCG GCGAGCAAAG CCAGCCCACT GGTCTTCGAG 
TCCCACAGCA TGGCCCAGGG TCACACCGCA GGCCGGAAGT CCAAGGGGGC CAGACAGGGC 
CAGCACAGCA GCCAGCACCA TGGCCACAGC CCCCTGGAAC AGCACTCGCC CCCGCCGCCG 
CCGCCAGTGC CTCAGCAGCA GGAGCCACAG CCCGAGAGGC TGTCCCCTGC GCCCCTCGCA 
CCCCCGCCCC ACCCAGAGCA CCCCGGCAGC ACCCGGCACA GCTCCGAGGA CTCAGACATC 
ACCAGTCTCA TCGAAGCCAT GGACAAAGAC TTCGAGCACC ACGACGCCCC GCCCCTAGAT 
GTGTTTACAG AGCAGCCGCC TTCACCCTTG TCGAAAAGCA AAGGGAAAGG AAAGCCTCCT 
CAGCGCAAGG TGAAGCCCCC CAAGAAGCAG GAGGAGAAGG AGAAGAAGGG AAAGGGGAGG 
GCCCTGGAAG ACGAGCTGAA GGACTCCTTG GCTGACGACG ACAGCTCCTC CACCACCACA 
GAGACCTCCA ACCCAGACAC AGAGCCTCTC CTCAAGGAGG ATACAGAAAA GCAAAAGGGA 
AAACAAGCCA TGCCTGAAAA ACATGAAAAC GAAATGTCTC AAGTGAAACA AAAAAGCAAA 
AAAGTCTTAA ATGTTAAGAA AGAAATCCCA GCAGATGTGA AACCCAGTTG CTTAGAGCTA 
CCGTATACTG CCCCCTTGGA AAGCAAACAG CGCCGAAATC TCCCAACCAA GATTCCTCTT 
CCAACTACAC TGACCAGTGG ATCCAAACCA CGAAACTCCC AGAAAACAAA AGGTACAAGT 
AAGTTAGTGG ATAGCAGGCC ACCTGCCCTC GCAAAGTTTC TCCCAAACAG TCAAGAACTA 
GGCAACACCA GCAGCTCAGA AGGTGAAAAA GACTCTCCAC CACCAGAGTG GGATTCCGTG 
CCTGTTCACA AGCCCGGCAG CTCTACAGAT AGTCTTTATA AACTCTCTCT GCAAACCCTC 
AACGCAGACA TCTTCCTGAA ACAACGCCAG ACCTCGCCGC CGCCCACCTC CCCCTCACCG 
CCCGCCGCAC CCTGCCCGTT CACGTCCCGG GGCAGCTACA GCAGCATCGT CCATGGCAGC 
CCGGGCAGCG ACTCTAAAGC AAAGCAGCCA AGCGGGAGCA AACACAAACT GACAAAAGCC 
GCTTCTCTCC CGGGCAAGAA TGGCAACCCC ACTTTCGCTG CGGTCACGGC TGGGTATGAC 
AAGAGCCCAG GTGGGAACGG CTTTGCCAAA GTTTCTTCAA GCAAAACAGA TTTCTCTAGC 
AGCCTTGGCA TTTCACACAC TCCTGTTGAC AGCGATGGCT CAGACAGCTC GGGTCTGTGG 
AGCCCCGTGA GCAACCCCAG CAGCCCTGAC TTCACACCCC TCAACTCGTT CTCAGCCTTT 
GGGAATTCTT TTAACCTAAC TGGTGAAGTG TTCAGCAAGC TCGGGTTATC GCGATCATGC 
AATCAGCCCT CACAGAGGAG CTGGAATGAG CTGAGCAGCG GCCCCTCCTA CCTCTGGGAC 
TCGCCGGCCA CAGAGCCCAG CCCTTCCTGG CCAGCCAGTT CCAGCTCCCC GACCCACACG 
GCCACACCCA TCCTTGGCAG CACCAGCAGC CTGTGGTCGT CCACTCCGTT CAGCAGCTCC 
ATCTGGTCCA GCAGCCTGCA CAGCACCCTG CCCTTCACCA GCCCCACCAG CGCGTTGCCG 
AGCATCAGCC TCATGGGCCC CGAGAGCGCC CCGGCAGCTC ACGCGCCCTC TGCCGCCAGC 
CCCTCCGACG ACCTGGGCCA GACCTACAAC CCCTGGCGGA TATGGAGTCC CACCATCGGA 
CGGAGGAGCT CAGACCCCTG GTCGAATTCG CACTTCCCTC ACGAGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM131 ( XM_613474.9 ) cDNA ORF clone, Bos taurus(cattle) -> XP_613474.6 Bos taurus transmembrane protein 131 (TMEM131), transcript variant X1, mRNA.

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CloneID	OBa06771
Clone ID Related Accession (Same CDS sequence)	XM_002691141.5 , XM_002691141.6 , XM_613474.9
Accession Version	XM_613474.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transmembrane protein 131 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645888.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_613474.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGC GGGCCGGAGG AGGAGCAGCG GGATCCGCCG CCGCTGCCTC CACGTCCGCC 
GTGGCCGGTC TGGAGCCCGC GGCCGGCCGC GGCGGGGGCC CGCGGAGCGC AGCCGCCGGC 
CTCCTGGGAG CGCTGCACCT GGTGATGACC CTCGTCGTGG CCGCGGCGCG GGCCGAGAAG 
GAAGCATTTA TTCAGTCGGA GAGCATAATA GAAGTGCTGC GTTTTGATGA TGGAGGGCTT 
CTACAGACTG AGACAACACT TGGACTCAGT TCATACCAAC AGAAAAGTAT ATCTTTGTAC 
CGGGGTAATT GCAGACCTAT ACGGTTTGAG CCACCAATGC TGGATTTCCA TGAACAACCA 
GTTGGAATGC CAAAAATGGA AAAAGTTTAC TTACATAATC CTAGTTCTGA AGAAACTATT 
ACTTTAGTAT CAATATCTGC TACCACATCA CATTTTCACG CATCATTTTT TCAAAATAGG 
AAAATTCTTC CAGGAGGAAA TACATCATTT GATGTAGTTT TTCTTGCAAG AGTAGTGGGA 
AATGTAGAAA ATACTTTATT TATTAATACA TCTAATCATG GGGTATTCAC TTACCAGGTA 
TTTGGTGTTG GAGTTCCAAA TCCATACCGA TTGAGGCCAT TCCTTGGGGC CAGAGTCCCT 
GTGAATAGCA GTTTCTCACC CATAATAAAC ATACACAATC CTCACAGCGA GCCTTTACAG 
GTTGTAGAAA TGTACTCTAG TGGAGGAGAC CTTCACCTTG AACTTCCCAC AGGGCAGCAG 
GGAGGCACCA GGAAGCTGTG GGAAATTCCT CCTTATGAAA CCAAGGGAGT GATGAGAGCC 
AGTTTTTCAT CTAGAGAAGC AGATAATCAC ACAGCTTTCA TAAGAATAAA GACAAATGCT 
TCAGACAGCA CGGAGTTTAT CATCCTTCCG GTTGAAGTGG AAGTCACAAC AGCTCCTGGA 
ATTTATTCCT CAACTGAAAT GTTAGATTTT GGCACACTGC GAACACAAGA TCTACCAAAA 
GTTTTAAATC TTCATTTATT AAATTCAGGA ACAAAAGATG TACCAATAAC AAGTGTTCGA 
CCTACACCAC AAAATGATGC TGTCACTGTA CACTTTAAAC CAATTACATT AAAAGCTTCA 
GAAAGTAAAT ATACCAAGGT AGCAAGTATT AGTTTTGATG CATCAAAGGC GAAAAAACCA 
TCTCAGTTTT CTGGGAAAAT AACTGTGAAA GCAAAGGAAA AGAGTTATTC TAAACTTGAA 
ATACCATATC AAGCAGAGGT TTTAGATGGT TATTTGGGAT TTGATCACGC TGCAACATTA 
TTTCACATTC GAGACAGTCC TGCTGATCCT GTGGAAAGGC CAATTTACCT TACTAACACT 
TTCAGCTTTG CGATCCTCAT TCATGATGTG TTGCTACCAG AAGAAGCCAA AACAATGTTT 
AAAGTTCACA ACTTCAGCAA ACCAGTCTTA ATTCTACCAA ATGGATCAGG CTACATTTTT 
ACTCTATTTT TTATGCCATC CACATCGTCC ATGCACATAG ATAACAATAT TTTACTTATT 
ACCAATGCCT CTAAGTTTCA CTTACCTGTG CGGGTATATA CAGGCTTTTT AGATTACTTT 
GTATTACCCC CCAAAATAGA AGAACGTTTC ATAGATTTTG GTATACTGAG TGCAACAGAA 
GCAAGTAATA TTTTATTTGC CATTATAAAC AGCAATCCAA TTGAGTTGGC TATAAAAAGC 
TGGCATATTA TAGGAGATGG TTTATCAATA GAACTCGTAG CTACTGAAAG AGGCAATAGA 
ACTACAATCA TCTCAAGCCT TCCAGAGTTT GAAAAATCCT CTCTATCAGA CCAGTCATCG 
GTAATACTAG CTTCAGGATA CTTTGCAGTG TTCAGAGTCA AACTTACTGC AAAAAAACTG 
GAAGGAATTC ATGATGGGGC TATACAGATC ACGACAGACT ATGAGATCCT AACAATTCCT 
GTGAAGGCTG TGATCGCAGT GGGTTCTTTG ACTTGCTTCC CAAAGCACAT GGTTCTTCCA 
CCTTCCTTTC CAGGGAAAAT AGTTCATCAG AGTTTAAATA TTATGAATTC CTTCTCACAG 
AAGGTAAAGA TCCAACAGAT ACGATCTTTG TCAGAAGACG TGCGTTTTTA CTACAAACGA 
TTGCGGGGTA ATAAGGAAGA CTTGGAGCCA GGGAAAAAAT CAAAGATTGC AAACATTTAC 
TTTGATCCTG GATTGCAGTG CGGGGATCAT TGCTATGTCG GCTTGCCTTT TCTGTCCAAA 
TCTGAACCCA GAGTCCAGCC TGGCATAGCC ATGCAGGAAG ATGCCTGGGA CACCGACTGG 
GATTTGCATC AAAGCCTATT CAAGGGATGG ATGGGAATAA AGGAAAACTC AGGTCACAAA 
TTGAGCGCTA TGTTTGAAGT GAATACAGAC CTTCAGAAAA ACATCCTGTC AAAAGTTTCT 
GCCGAGCTCT CCTGGCCATC CCTCCTCAGC TCCCCCCGGC TCGTGAGGTT TCCCCTCACT 
AATACAAACT GCTCTTCAGA AGAAGAGATT ATGTTAGAAA ACCCTGCAGA TGTTCCCATC 
TATATTCAAT TTATCCCCCT GGCTTTATAT TCCAGCCCTT CCATCTTTGT AGATAAGTTA 
ATATCAAGGT TTAACTTAAG TAAGGTGGCA AAGATCGATT TAAGAACACA AGAATTCCAA 
GTCTTTAGAA ACACTGCTCA CCCACTGCAG AGTTCAACAG GATTCACAGA GGGCCTCTCC 
CGACATTTAG TTTTAAACTT AATTTTAAAA CCTGGAGAAA AGAAATCTGT CAAAGTAAAG 
TTTACTCCAG TTCACAATAG AACTGTTTCT TCCCTAATCA TAATCAGAAA TAACCTGACT 
GTGATGGATG CCGTAATGGT CCAAGGACAG GGGACAACCG AGAACCTGAG GGTGGCAGGC 
AAGCTTCCGG GCCCAGGGAG CTCCTTACGC TTCAAAATCA CAGAAGCACT GCTGAAAGAC 
TGTACGGAGG GTATGAGACT AAGGGAACCC AATTTCACAT TGAAAAGAAC ATTTAAAGTA 
GAGAACACAG GACAACTTCC AATTCACATA GAAACCATTG AAGTCAGTGG GTACTCTTGT 
GAGGGATATG GCTTTAAAGT CGTCAATTGT CAAGAGTTTG CACTGAGCGC CAATGCCTCT 
AAAGACATAG TCATTCTGTT TACTCCTGAT TTCACGGCTT CTAGAGTCAT CCGTGAACTG 
AAGTTTATAA CAGCCAGTGG CTCTGAGTTT GTGTTTGTAT TGAATGCATC CCTTCCTTAC 
CATATGTTAG CAACCTGTGC AGAAGCCCTG CCCAGGCCCA ACTGGGAGCT GGCTCTGTAC 
ATCATCATCT CAGGAATAAT GAGTGCGCTG TTTCTCTTGG TAATTGGAAC AGCCTATTTA 
GAAGCTCAAG GAATTTGGGA GCCCTTCCGA AGGCGGCTGT CCTTTGAGGC CTCGAACCCG 
CCCTTTGATG TGGGGAGACC ATTTGATCTC AGGAGAATCG TTGGTATTTC ATCTGAAGGA 
AACTTGAACA CACTCAGCTG TGACCCCGGT CACAGTCGGG GCTTCTGTGG AGCAGGTGGC 
TCGGCCTCCC GGCCCGGCGC AGGGAGTCAC AAGCAGTGTA GCCCGTCGGT CCACCCGCAC 
AGCAGTCACA GCAATAGAAA CTCGGCTGAC GTGGAAAACG TCAGAGCCAA AAACAGTGCG 
AGTGCCTCTG GCAGGACTAA TGCCCAGTCG GCGAGCAAAG CCAGCCCACT GGTCTTCGAG 
TCCCACAGCA TGGCCCAGGG TCACACCGCA GGCCGGAAGT CCAAGGGGGC CAGACAGGGC 
CAGCACAGCA GCCAGCACCA TGGCCACAGC CCCCTGGAAC AGCACTCGCC CCCGCCGCCG 
CCGCCAGTGC CTCAGCAGCA GGAGCCACAG CCCGAGAGGC TGTCCCCTGC GCCCCTCGCA 
CCCCCGCCCC ACCCAGAGCA CCCCGGCAGC ACCCGGCACA GCTCCGAGGA CTCAGACATC 
ACCAGTCTCA TCGAAGCCAT GGACAAAGAC TTCGAGCACC ACGACGCCCC GCCCCTAGAT 
GTGTTTACAG AGCAGCCGCC TTCACCCTTG TCGAAAAGCA AAGGGAAAGG AAAGCCTCCT 
CAGCGCAAGG TGAAGCCCCC CAAGAAGCAG GAGGAGAAGG AGAAGAAGGG AAAGGGGAGG 
GCCCTGGAAG ACGAGCTGAA GGACTCCTTG GCTGACGACG ACAGCTCCTC CACCACCACA 
GAGACCTCCA ACCCAGACAC AGAGCCTCTC CTCAAGGAGG ATACAGAAAA GCAAAAGGGA 
AAACAAGCCA TGCCTGAAAA ACATGAAAAC GAAATGTCTC AAGTGAAACA AAAAAGCAAA 
AAAGTCTTAA ATGTTAAGAA AGAAATCCCA GCAGATGTGA AACCCAGTTG CTTAGAGCTA 
CCGTATACTG CCCCCTTGGA AAGCAAACAG CGCCGAAATC TCCCAACCAA GATTCCTCTT 
CCAACTACAC TGACCAGTGG ATCCAAACCA CGAAACTCCC AGAAAACAAA AGGTACAAGT 
AAGTTAGTGG ATAGCAGGCC ACCTGCCCTC GCAAAGTTTC TCCCAAACAG TCAAGAACTA 
GGCAACACCA GCAGCTCAGA AGGTGAAAAA GACTCTCCAC CACCAGAGTG GGATTCCGTG 
CCTGTTCACA AGCCCGGCAG CTCTACAGAT AGTCTTTATA AACTCTCTCT GCAAACCCTC 
AACGCAGACA TCTTCCTGAA ACAACGCCAG ACCTCGCCGC CGCCCACCTC CCCCTCACCG 
CCCGCCGCAC CCTGCCCGTT CACGTCCCGG GGCAGCTACA GCAGCATCGT CCATGGCAGC 
CCGGGCAGCG ACTCTAAAGC AAAGCAGCCA AGCGGGAGCA AACACAAACT GACAAAAGCC 
GCTTCTCTCC CGGGCAAGAA TGGCAACCCC ACTTTCGCTG CGGTCACGGC TGGGTATGAC 
AAGAGCCCAG GTGGGAACGG CTTTGCCAAA GTTTCTTCAA GCAAAACAGA TTTCTCTAGC 
AGCCTTGGCA TTTCACACAC TCCTGTTGAC AGCGATGGCT CAGACAGCTC GGGTCTGTGG 
AGCCCCGTGA GCAACCCCAG CAGCCCTGAC TTCACACCCC TCAACTCGTT CTCAGCCTTT 
GGGAATTCTT TTAACCTAAC TGGTGAAGTG TTCAGCAAGC TCGGGTTATC GCGATCATGC 
AATCAGCCCT CACAGAGGAG CTGGAATGAG CTGAGCAGCG GCCCCTCCTA CCTCTGGGAC 
TCGCCGGCCA CAGAGCCCAG CCCTTCCTGG CCAGCCAGTT CCAGCTCCCC GACCCACACG 
GCCACACCCA TCCTTGGCAG CACCAGCAGC CTGTGGTCGT CCACTCCGTT CAGCAGCTCC 
ATCTGGTCCA GCAGCCTGCA CAGCACCCTG CCCTTCACCA GCCCCACCAG CGCGTTGCCG 
AGCATCAGCC TCATGGGCCC CGAGAGCGCC CCGGCAGCTC ACGCGCCCTC TGCCGCCAGC 
CCCTCCGACG ACCTGGGCCA GACCTACAAC CCCTGGCGGA TATGGAGTCC CACCATCGGA 
CGGAGGAGCT CAGACCCCTG GTCGAATTCG CACTTCCCTC ACGAGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_613474.6
CDS	558..6188
Translation	MGKRAGGGAAGSAAAASTSAVAGLEPAAGRGGGPRSAAAGLLGALHLVMTLVVAAARAEKEAFIQSESIIEVLRFDDGGLLQTETTLGLSSYQQKSISLYRGNCRPIRFEPPMLDFHEQPVGMPKMEKVYLHNPSSEETITLVSISATTSHFHASFFQNRKILPGGNTSFDVVFLARVVGNVENTLFINTSNHGVFTYQVFGVGVPNPYRLRPFLGARVPVNSSFSPIINIHNPHSEPLQVVEMYSSGGDLHLELPTGQQGGTRKLWEIPPYETKGVMRASFSSREADNHTAFIRIKTNASDSTEFIILPVEVEVTTAPGIYSSTEMLDFGTLRTQDLPKVLNLHLLNSGTKDVPITSVRPTPQNDAVTVHFKPITLKASESKYTKVASISFDASKAKKPSQFSGKITVKAKEKSYSKLEIPYQAEVLDGYLGFDHAATLFHIRDSPADPVERPIYLTNTFSFAILIHDVLLPEEAKTMFKVHNFSKPVLILPNGSGYIFTLFFMPSTSSMHIDNNILLITNASKFHLPVRVYTGFLDYFVLPPKIEERFIDFGILSATEASNILFAIINSNPIELAIKSWHIIGDGLSIELVATERGNRTTIISSLPEFEKSSLSDQSSVILASGYFAVFRVKLTAKKLEGIHDGAIQITTDYEILTIPVKAVIAVGSLTCFPKHMVLPPSFPGKIVHQSLNIMNSFSQKVKIQQIRSLSEDVRFYYKRLRGNKEDLEPGKKSKIANIYFDPGLQCGDHCYVGLPFLSKSEPRVQPGIAMQEDAWDTDWDLHQSLFKGWMGIKENSGHKLSAMFEVNTDLQKNILSKVSAELSWPSLLSSPRLVRFPLTNTNCSSEEEIMLENPADVPIYIQFIPLALYSSPSIFVDKLISRFNLSKVAKIDLRTQEFQVFRNTAHPLQSSTGFTEGLSRHLVLNLILKPGEKKSVKVKFTPVHNRTVSSLIIIRNNLTVMDAVMVQGQGTTENLRVAGKLPGPGSSLRFKITEALLKDCTEGMRLREPNFTLKRTFKVENTGQLPIHIETIEVSGYSCEGYGFKVVNCQEFALSANASKDIVILFTPDFTASRVIRELKFITASGSEFVFVLNASLPYHMLATCAEALPRPNWELALYIIISGIMSALFLLVIGTAYLEAQGIWEPFRRRLSFEASNPPFDVGRPFDLRRIVGISSEGNLNTLSCDPGHSRGFCGAGGSASRPGAGSHKQCSPSVHPHSSHSNRNSADVENVRAKNSASASGRTNAQSASKASPLVFESHSMAQGHTAGRKSKGARQGQHSSQHHGHSPLEQHSPPPPPPVPQQQEPQPERLSPAPLAPPPHPEHPGSTRHSSEDSDITSLIEAMDKDFEHHDAPPLDVFTEQPPSPLSKSKGKGKPPQRKVKPPKKQEEKEKKGKGRALEDELKDSLADDDSSSTTTETSNPDTEPLLKEDTEKQKGKQAMPEKHENEMSQVKQKSKKVLNVKKEIPADVKPSCLELPYTAPLESKQRRNLPTKIPLPTTLTSGSKPRNSQKTKGTSKLVDSRPPALAKFLPNSQELGNTSSSEGEKDSPPPEWDSVPVHKPGSSTDSLYKLSLQTLNADIFLKQRQTSPPPTSPSPPAAPCPFTSRGSYSSIVHGSPGSDSKAKQPSGSKHKLTKAASLPGKNGNPTFAAVTAGYDKSPGGNGFAKVSSSKTDFSSSLGISHTPVDSDGSDSSGLWSPVSNPSSPDFTPLNSFSAFGNSFNLTGEVFSKLGLSRSCNQPSQRSWNELSSGPSYLWDSPATEPSPSWPASSSSPTHTATPILGSTSSLWSSTPFSSSIWSSSLHSTLPFTSPTSALPSISLMGPESAPAAHAPSAASPSDDLGQTYNPWRIWSPTIGRRSSDPWSNSHFPHEN

Target ORF information:

RefSeq Version	XM_613474.9
Organism	Bos taurus(cattle)
Definition	Bos taurus transmembrane protein 131 (TMEM131), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_613474.9

ORF Insert Sequence:

ATGGGGAAGC GGGCCGGAGG AGGAGCAGCG GGATCCGCCG CCGCTGCCTC CACGTCCGCC 
GTGGCCGGTC TGGAGCCCGC GGCCGGCCGC GGCGGGGGCC CGCGGAGCGC AGCCGCCGGC 
CTCCTGGGAG CGCTGCACCT GGTGATGACC CTCGTCGTGG CCGCGGCGCG GGCCGAGAAG 
GAAGCATTTA TTCAGTCGGA GAGCATAATA GAAGTGCTGC GTTTTGATGA TGGAGGGCTT 
CTACAGACTG AGACAACACT TGGACTCAGT TCATACCAAC AGAAAAGTAT ATCTTTGTAC 
CGGGGTAATT GCAGACCTAT ACGGTTTGAG CCACCAATGC TGGATTTCCA TGAACAACCA 
GTTGGAATGC CAAAAATGGA AAAAGTTTAC TTACATAATC CTAGTTCTGA AGAAACTATT 
ACTTTAGTAT CAATATCTGC TACCACATCA CATTTTCACG CATCATTTTT TCAAAATAGG 
AAAATTCTTC CAGGAGGAAA TACATCATTT GATGTAGTTT TTCTTGCAAG AGTAGTGGGA 
AATGTAGAAA ATACTTTATT TATTAATACA TCTAATCATG GGGTATTCAC TTACCAGGTA 
TTTGGTGTTG GAGTTCCAAA TCCATACCGA TTGAGGCCAT TCCTTGGGGC CAGAGTCCCT 
GTGAATAGCA GTTTCTCACC CATAATAAAC ATACACAATC CTCACAGCGA GCCTTTACAG 
GTTGTAGAAA TGTACTCTAG TGGAGGAGAC CTTCACCTTG AACTTCCCAC AGGGCAGCAG 
GGAGGCACCA GGAAGCTGTG GGAAATTCCT CCTTATGAAA CCAAGGGAGT GATGAGAGCC 
AGTTTTTCAT CTAGAGAAGC AGATAATCAC ACAGCTTTCA TAAGAATAAA GACAAATGCT 
TCAGACAGCA CGGAGTTTAT CATCCTTCCG GTTGAAGTGG AAGTCACAAC AGCTCCTGGA 
ATTTATTCCT CAACTGAAAT GTTAGATTTT GGCACACTGC GAACACAAGA TCTACCAAAA 
GTTTTAAATC TTCATTTATT AAATTCAGGA ACAAAAGATG TACCAATAAC AAGTGTTCGA 
CCTACACCAC AAAATGATGC TGTCACTGTA CACTTTAAAC CAATTACATT AAAAGCTTCA 
GAAAGTAAAT ATACCAAGGT AGCAAGTATT AGTTTTGATG CATCAAAGGC GAAAAAACCA 
TCTCAGTTTT CTGGGAAAAT AACTGTGAAA GCAAAGGAAA AGAGTTATTC TAAACTTGAA 
ATACCATATC AAGCAGAGGT TTTAGATGGT TATTTGGGAT TTGATCACGC TGCAACATTA 
TTTCACATTC GAGACAGTCC TGCTGATCCT GTGGAAAGGC CAATTTACCT TACTAACACT 
TTCAGCTTTG CGATCCTCAT TCATGATGTG TTGCTACCAG AAGAAGCCAA AACAATGTTT 
AAAGTTCACA ACTTCAGCAA ACCAGTCTTA ATTCTACCAA ATGGATCAGG CTACATTTTT 
ACTCTATTTT TTATGCCATC CACATCGTCC ATGCACATAG ATAACAATAT TTTACTTATT 
ACCAATGCCT CTAAGTTTCA CTTACCTGTG CGGGTATATA CAGGCTTTTT AGATTACTTT 
GTATTACCCC CCAAAATAGA AGAACGTTTC ATAGATTTTG GTATACTGAG TGCAACAGAA 
GCAAGTAATA TTTTATTTGC CATTATAAAC AGCAATCCAA TTGAGTTGGC TATAAAAAGC 
TGGCATATTA TAGGAGATGG TTTATCAATA GAACTCGTAG CTACTGAAAG AGGCAATAGA 
ACTACAATCA TCTCAAGCCT TCCAGAGTTT GAAAAATCCT CTCTATCAGA CCAGTCATCG 
GTAATACTAG CTTCAGGATA CTTTGCAGTG TTCAGAGTCA AACTTACTGC AAAAAAACTG 
GAAGGAATTC ATGATGGGGC TATACAGATC ACGACAGACT ATGAGATCCT AACAATTCCT 
GTGAAGGCTG TGATCGCAGT GGGTTCTTTG ACTTGCTTCC CAAAGCACAT GGTTCTTCCA 
CCTTCCTTTC CAGGGAAAAT AGTTCATCAG AGTTTAAATA TTATGAATTC CTTCTCACAG 
AAGGTAAAGA TCCAACAGAT ACGATCTTTG TCAGAAGACG TGCGTTTTTA CTACAAACGA 
TTGCGGGGTA ATAAGGAAGA CTTGGAGCCA GGGAAAAAAT CAAAGATTGC AAACATTTAC 
TTTGATCCTG GATTGCAGTG CGGGGATCAT TGCTATGTCG GCTTGCCTTT TCTGTCCAAA 
TCTGAACCCA GAGTCCAGCC TGGCATAGCC ATGCAGGAAG ATGCCTGGGA CACCGACTGG 
GATTTGCATC AAAGCCTATT CAAGGGATGG ATGGGAATAA AGGAAAACTC AGGTCACAAA 
TTGAGCGCTA TGTTTGAAGT GAATACAGAC CTTCAGAAAA ACATCCTGTC AAAAGTTTCT 
GCCGAGCTCT CCTGGCCATC CCTCCTCAGC TCCCCCCGGC TCGTGAGGTT TCCCCTCACT 
AATACAAACT GCTCTTCAGA AGAAGAGATT ATGTTAGAAA ACCCTGCAGA TGTTCCCATC 
TATATTCAAT TTATCCCCCT GGCTTTATAT TCCAGCCCTT CCATCTTTGT AGATAAGTTA 
ATATCAAGGT TTAACTTAAG TAAGGTGGCA AAGATCGATT TAAGAACACA AGAATTCCAA 
GTCTTTAGAA ACACTGCTCA CCCACTGCAG AGTTCAACAG GATTCACAGA GGGCCTCTCC 
CGACATTTAG TTTTAAACTT AATTTTAAAA CCTGGAGAAA AGAAATCTGT CAAAGTAAAG 
TTTACTCCAG TTCACAATAG AACTGTTTCT TCCCTAATCA TAATCAGAAA TAACCTGACT 
GTGATGGATG CCGTAATGGT CCAAGGACAG GGGACAACCG AGAACCTGAG GGTGGCAGGC 
AAGCTTCCGG GCCCAGGGAG CTCCTTACGC TTCAAAATCA CAGAAGCACT GCTGAAAGAC 
TGTACGGAGG GTATGAGACT AAGGGAACCC AATTTCACAT TGAAAAGAAC ATTTAAAGTA 
GAGAACACAG GACAACTTCC AATTCACATA GAAACCATTG AAGTCAGTGG GTACTCTTGT 
GAGGGATATG GCTTTAAAGT CGTCAATTGT CAAGAGTTTG CACTGAGCGC CAATGCCTCT 
AAAGACATAG TCATTCTGTT TACTCCTGAT TTCACGGCTT CTAGAGTCAT CCGTGAACTG 
AAGTTTATAA CAGCCAGTGG CTCTGAGTTT GTGTTTGTAT TGAATGCATC CCTTCCTTAC 
CATATGTTAG CAACCTGTGC AGAAGCCCTG CCCAGGCCCA ACTGGGAGCT GGCTCTGTAC 
ATCATCATCT CAGGAATAAT GAGTGCGCTG TTTCTCTTGG TAATTGGAAC AGCCTATTTA 
GAAGCTCAAG GAATTTGGGA GCCCTTCCGA AGGCGGCTGT CCTTTGAGGC CTCGAACCCG 
CCCTTTGATG TGGGGAGACC ATTTGATCTC AGGAGAATCG TTGGTATTTC ATCTGAAGGA 
AACTTGAACA CACTCAGCTG TGACCCCGGT CACAGTCGGG GCTTCTGTGG AGCAGGTGGC 
TCGGCCTCCC GGCCCGGCGC AGGGAGTCAC AAGCAGTGTA GCCCGTCGGT CCACCCGCAC 
AGCAGTCACA GCAATAGAAA CTCGGCTGAC GTGGAAAACG TCAGAGCCAA AAACAGTGCG 
AGTGCCTCTG GCAGGACTAA TGCCCAGTCG GCGAGCAAAG CCAGCCCACT GGTCTTCGAG 
TCCCACAGCA TGGCCCAGGG TCACACCGCA GGCCGGAAGT CCAAGGGGGC CAGACAGGGC 
CAGCACAGCA GCCAGCACCA TGGCCACAGC CCCCTGGAAC AGCACTCGCC CCCGCCGCCG 
CCGCCAGTGC CTCAGCAGCA GGAGCCACAG CCCGAGAGGC TGTCCCCTGC GCCCCTCGCA 
CCCCCGCCCC ACCCAGAGCA CCCCGGCAGC ACCCGGCACA GCTCCGAGGA CTCAGACATC 
ACCAGTCTCA TCGAAGCCAT GGACAAAGAC TTCGAGCACC ACGACGCCCC GCCCCTAGAT 
GTGTTTACAG AGCAGCCGCC TTCACCCTTG TCGAAAAGCA AAGGGAAAGG AAAGCCTCCT 
CAGCGCAAGG TGAAGCCCCC CAAGAAGCAG GAGGAGAAGG AGAAGAAGGG AAAGGGGAGG 
GCCCTGGAAG ACGAGCTGAA GGACTCCTTG GCTGACGACG ACAGCTCCTC CACCACCACA 
GAGACCTCCA ACCCAGACAC AGAGCCTCTC CTCAAGGAGG ATACAGAAAA GCAAAAGGGA 
AAACAAGCCA TGCCTGAAAA ACATGAAAAC GAAATGTCTC AAGTGAAACA AAAAAGCAAA 
AAAGTCTTAA ATGTTAAGAA AGAAATCCCA GCAGATGTGA AACCCAGTTG CTTAGAGCTA 
CCGTATACTG CCCCCTTGGA AAGCAAACAG CGCCGAAATC TCCCAACCAA GATTCCTCTT 
CCAACTACAC TGACCAGTGG ATCCAAACCA CGAAACTCCC AGAAAACAAA AGGTACAAGT 
AAGTTAGTGG ATAGCAGGCC ACCTGCCCTC GCAAAGTTTC TCCCAAACAG TCAAGAACTA 
GGCAACACCA GCAGCTCAGA AGGTGAAAAA GACTCTCCAC CACCAGAGTG GGATTCCGTG 
CCTGTTCACA AGCCCGGCAG CTCTACAGAT AGTCTTTATA AACTCTCTCT GCAAACCCTC 
AACGCAGACA TCTTCCTGAA ACAACGCCAG ACCTCGCCGC CGCCCACCTC CCCCTCACCG 
CCCGCCGCAC CCTGCCCGTT CACGTCCCGG GGCAGCTACA GCAGCATCGT CCATGGCAGC 
CCGGGCAGCG ACTCTAAAGC AAAGCAGCCA AGCGGGAGCA AACACAAACT GACAAAAGCC 
GCTTCTCTCC CGGGCAAGAA TGGCAACCCC ACTTTCGCTG CGGTCACGGC TGGGTATGAC 
AAGAGCCCAG GTGGGAACGG CTTTGCCAAA GTTTCTTCAA GCAAAACAGA TTTCTCTAGC 
AGCCTTGGCA TTTCACACAC TCCTGTTGAC AGCGATGGCT CAGACAGCTC GGGTCTGTGG 
AGCCCCGTGA GCAACCCCAG CAGCCCTGAC TTCACACCCC TCAACTCGTT CTCAGCCTTT 
GGGAATTCTT TTAACCTAAC TGGTGAAGTG TTCAGCAAGC TCGGGTTATC GCGATCATGC 
AATCAGCCCT CACAGAGGAG CTGGAATGAG CTGAGCAGCG GCCCCTCCTA CCTCTGGGAC 
TCGCCGGCCA CAGAGCCCAG CCCTTCCTGG CCAGCCAGTT CCAGCTCCCC GACCCACACG 
GCCACACCCA TCCTTGGCAG CACCAGCAGC CTGTGGTCGT CCACTCCGTT CAGCAGCTCC 
ATCTGGTCCA GCAGCCTGCA CAGCACCCTG CCCTTCACCA GCCCCACCAG CGCGTTGCCG 
AGCATCAGCC TCATGGGCCC CGAGAGCGCC CCGGCAGCTC ACGCGCCCTC TGCCGCCAGC 
CCCTCCGACG ACCTGGGCCA GACCTACAAC CCCTGGCGGA TATGGAGTCC CACCATCGGA 
CGGAGGAGCT CAGACCCCTG GTCGAATTCG CACTTCCCTC ACGAGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM131 ( XM_005193810.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005193867.1 Bos taurus transmembrane protein 131 (TMEM131), transcript variant X2, mRNA.

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CloneID	OBa06772
Clone ID Related Accession (Same CDS sequence)	XM_005193810.3 , XM_005212403.3 , XM_005212403.4
Accession Version	XM_005193810.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transmembrane protein 131 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645888.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005193810.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGC GGGCCGGAGG AGGAGCAGCG GGATCCGCCG CCGCTGCCTC CACGTCCGCC 
GTGGCCGGTC TGGAGCCCGC GGCCGGCCGC GGCGGGGGCC CGCGGAGCGC AGCCGCCGGC 
CTCCTGGGAG CGCTGCACCT GGTGATGACC CTCGTCGTGG CCGCGGCGCG GGCCGAGAAG 
GAAGCATTTA TTCAGTCGGA GAGCATAATA GAAGTGCTGC GTTTTGATGA TGGAGGGCTT 
CTACAGACTG AGACAACACT TGGACTCAGT TCATACCAAC AGAAAAGTAT ATCTTTGTAC 
CGGGGTAATT GCAGACCTAT ACGGTTTGAG CCACCAATGC TGGATTTCCA TGAACAACCA 
GTTGGAATGC CAAAAATGGA AAAAGTTTAC TTACATAATC CTAGTTCTGA AGAAACTATT 
ACTTTAGTAT CAATATCTGC TACCACATCA CATTTTCACG CATCATTTTT TCAAAATAGG 
AAAATTCTTC CAGGAGGAAA TACATCATTT GATGTAGTTT TTCTTGCAAG AGTAGTGGGA 
AATGTAGAAA ATACTTTATT TATTAATACA TCTAATCATG GGGTATTCAC TTACCAGGTA 
TTTGGTGTTG GAGTTCCAAA TCCATACCGA TTGAGGCCAT TCCTTGGGGC CAGAGTCCCT 
GTGAATAGCA GTTTCTCACC CATAATAAAC ATACACAATC CTCACAGCGA GCCTTTACAG 
GTTGTAGAAA TGTACTCTAG TGGAGGAGAC CTTCACCTTG AACTTCCCAC AGGGCAGCAG 
GGAGGCACCA GGAAGCTGTG GGAAATTCCT CCTTATGAAA CCAAGGGAGT GATGAGAGCC 
AGTTTTTCAT CTAGAGAAGC AGATAATCAC ACAGCTTTCA TAAGAATAAA GACAAATGCT 
TCAGACAGCA CGGAGTTTAT CATCCTTCCG GTTGAAGTGG AAGTCACAAC AGCTCCTGGA 
ATTTATTCCT CAACTGAAAT GTTAGATTTT GGCACACTGC GAACACAAGA TCTACCAAAA 
GTTTTAAATC TTCATTTATT AAATTCAGGA ACAAAAGATG TACCAATAAC AAGTGTTCGA 
CCTACACCAC AAAATGATGC TGTCACTGTA CACTTTAAAC CAATTACATT AAAAGCTTCA 
GAAAGTAAAT ATACCAAGGT AGCAAGTATT AGTTTTGATG CATCAAAGGC GAAAAAACCA 
TCTCAGTTTT CTGGGAAAAT AACTGTGAAA GCAAAGGAAA AGAGTTATTC TAAACTTGAA 
ATACCATATC AAGCAGAGGT TTTAGATGGT TATTTGGGAT TTGATCACGC TGCAACATTA 
TTTCACATTC GAGACAGTCC TGCTGATCCT GTGGAAAGGC CAATTTACCT TACTAACACT 
TTCAGCTTTG CGATCCTCAT TCATGATGTG TTGCTACCAG AAGAAGCCAA AACAATGTTT 
AAAGTTCACA ACTTCAGCAA ACCAGTCTTA ATTCTACCAA ATGGATCAGG CTACATTTTT 
ACTCTATTTT TTATGCCATC CACATCGTCC ATGCACATAG ATAACAATAT TTTACTTATT 
ACCAATGCCT CTAAGTTTCA CTTACCTGTG CGGGTATATA CAGGCTTTTT AGATTACTTT 
GTATTACCCC CCAAAATAGA AGAACGTTTC ATAGATTTTG GTATACTGAG TGCAACAGAA 
GCAAGTAATA TTTTATTTGC CATTATAAAC AGCAATCCAA TTGAGTTGGC TATAAAAAGC 
TGGCATATTA TAGGAGATGG TTTATCAATA GAACTCGTAG CTACTGAAAG AGGCAATAGA 
ACTACAATCA TCTCAAGCCT TCCAGAGTTT GAAAAATCCT CTCTATCAGA CCAGTCATCG 
GTAATACTAG CTTCAGGATA CTTTGCAGTG TTCAGAGTCA AACTTACTGC AAAAAAACTG 
GAAGGAATTC ATGATGGGGC TATACAGATC ACGACAGACT ATGAGATCCT AACAATTCCT 
GTGAAGGCTG TGATCGCAGT GGGTTCTTTG ACTTGCTTCC CAAAGCACAT GGTTCTTCCA 
CCTTCCTTTC CAGGGAAAAT AGTTCATCAG AGTTTAAATA TTATGAATTC CTTCTCACAG 
AAGGTAAAGA TCCAACAGAT ACGATCTTTG TCAGAAGACG TGCGTTTTTA CTACAAACGA 
TTGCGGGGTA ATAAGGAAGA CTTGGAGCCA GGGAAAAAAT CAAAGATTGC AAACATTTAC 
TTTGATCCTG GATTGCAGTG CGGGGATCAT TGCTATGTCG GCTTGCCTTT TCTGTCCAAA 
TCTGAACCCA GAGTCCAGCC TGGCATAGCC ATGCAGGAAG ATGCCTGGGA CACCGACTGG 
GATTTGCATC AAAGCCTATT CAAGGGATGG ATGGGAATAA AGGAAAACTC AGGTCACAAA 
TTGAGCGCTA TGTTTGAAGT GAATACAGAC CTTCAGAAAA ACATCCTGTC AAAAGTTTCT 
GCCGAGCTCT CCTGGCCATC CCTCCTCAGC TCCCCCCGGC TCGTGAGGTT TCCCCTCACT 
AATACAAACT GCTCTTCAGA AGAAGAGATT ATGTTAGAAA ACCCTGCAGA TGTTCCCATC 
TATATTCAAT TTATCCCCCT GGCTTTATAT TCCAGCCCTT CCATCTTTGT AGATAAGTTA 
ATATCAAGGT TTAACTTAAG TAAGGTGGCA AAGATCGATT TAAGAACACA AGAATTCCAA 
GTCTTTAGAA ACACTGCTCA CCCACTGCAG AGTTCAACAG GATTCACAGA GGGCCTCTCC 
CGACATTTAG TTTTAAACTT AATTTTAAAA CCTGGAGAAA AGAAATCTGT CAAAGTAAAG 
TTTACTCCAG TTCACAATAG AACTGTTTCT TCCCTAATCA TAATCAGAAA TAACCTGACT 
GTGATGGATG CCGTAATGGT CCAAGGACAG GGGACAACCG AGAACCTGAG GGTGGCAGGC 
AAGCTTCCGG GCCCAGGGAG CTCCTTACGC TTCAAAATCA CAGAAGCACT GCTGAAAGAC 
TGTACGGAGG GTATGAGACT AAGGGAACCC AATTTCACAT TGAAAAGAAC ATTTAAAGTA 
GAGAACACAG GACAACTTCC AATTCACATA GAAACCATTG AAGTCAGTGG GTACTCTTGT 
GAGGGATATG GCTTTAAAGT CGTCAATTGT CAAGAGTTTG CACTGAGCGC CAATGCCTCT 
AAAGACATAG TCATTCTGTT TACTCCTGAT TTCACGGCTT CTAGAGTCAT CCGTGAACTG 
AAGTTTATAA CAGCCAGTGG CTCTGAGTTT GTGTTTGTAT TGAATGCATC CCTTCCTTAC 
CATATGTTAG CAACCTGTGC AGAAGCCCTG CCCAGGCCCA ACTGGGAGCT GGCTCTGTAC 
ATCATCATCT CAGGAATAAT GAGTGCGCTG TTTCTCTTGG TAATTGGAAC AGCCTATTTA 
GAAGCTCAAG GAATTTGGGA GCCCTTCCGA AGGCGGCTGT CCTTTGAGGC CTCGAACCCG 
CCCTTTGATG TGGGGAGACC ATTTGATCTC AGGAGAATCG TTGGTATTTC ATCTGAAGGA 
AACTTGAACA CACTCAGCTG TGACCCCGGT CACAGTCGGG GCTTCTGTGG AGCAGGTGGC 
TCGGCCTCCC GGCCCGGCGC AGGGAGTCAC AAGCAGTGTA GCCCGTCGGT CCACCCGCAC 
AGCAGTCACA GCAATAGAAA CTCGGCTGAC GTGGAAAACG TCAGAGCCAA AAACAGTGCG 
AGTGCCTCTG GCAGGACTAA TGCCCAGTCG GCGAGCAAAG CCAGCCCACT GGTCTTCGAG 
TCCCACAGCA TGGCCCAGGG TCACACCGCA GGCCGGAAGT CCAAGGGGGC CAGACAGGGC 
CAGCACAGCA GCCAGCACCA TGGCCACAGC CCCCTGGAAC AGCACTCGCC CCCGCCGCCG 
CCGCCAGTGC CTCAGCAGCA GGAGCCACAG CCCGAGAGGC TGTCCCCTGC GCCCCTCGCA 
CCCCCGCCCC ACCCAGAGCA CCCCGGCAGC ACCCGGCACA GCTCCGAGGA CTCAGACATC 
ACCAGTCTCA TCGAAGCCAT GGACAAAGAC TTCGAGCACC ACGACGCCCC GCCCCTAGAT 
GTGTTTACAG AGCAGCCGCC TTCACCCTTG TCGAAAAGCA AAGGGAAAGG AAAGCCTCCT 
CAGCGCAAGG TGAAGCCCCC CAAGAAGCAG GAGGAGAAGG AGAAGAAGGG AAAGGGGAGG 
GCCCTGGAAG ACGAGCTGAA GGACTCCTTG GCTGACGACG ACAGCTCCTC CACCACCACA 
GAGACCTCCA ACCCAGACAC AGAGCCTCTC CTCAAGGAGG ATACAGAAAA GCAAAAGGGA 
AAACAAGCCA TGCCTGAAAA ACATGAAAAC GAAATGTCTC AAGTGAAACA AAAAAGCAAA 
AAAGTCTTAA ATGTTAAGAA AGAAATCCCA GCAGATGTGA AACCCAGTTG CTTAGAGCTA 
CCGTATACTG CCCCCTTGGA AAGCAAACAG CGCCGAAATC TCCCAACCAA GATTCCTCTT 
CCAACTACAC TGACCAGTGG ATCCAAACCA CGAAACTCCC AGAAAACAAA AGGTACAAGT 
AAGTTAGTGG ATAGCAGGCC ACCTGCCCTC GCAAAGTTTC TCCCAAACAG TCAAGAACTA 
GGCAACACCA GCAGCTCAGA AGGTGAAAAA GACTCTCCAC CACCAGAGTG GGATTCCGTG 
CCTGTTCACA AGCCCGGCAG CTACATCTTC CTGAAACAAC GCCAGACCTC GCCGCCGCCC 
ACCTCCCCCT CACCGCCCGC CGCACCCTGC CCGTTCACGT CCCGGGGCAG CTACAGCAGC 
ATCGTCCATG GCAGCCCGGG CAGCGACTCT AAAGCAAAGC AGCCAAGCGG GAGCAAACAC 
AAACTGACAA AAGCCGCTTC TCTCCCGGGC AAGAATGGCA ACCCCACTTT CGCTGCGGTC 
ACGGCTGGGT ATGACAAGAG CCCAGGTGGG AACGGCTTTG CCAAAGTTTC TTCAAGCAAA 
ACAGATTTCT CTAGCAGCCT TGGCATTTCA CACACTCCTG TTGACAGCGA TGGCTCAGAC 
AGCTCGGGTC TGTGGAGCCC CGTGAGCAAC CCCAGCAGCC CTGACTTCAC ACCCCTCAAC 
TCGTTCTCAG CCTTTGGGAA TTCTTTTAAC CTAACTGGTG AAGTGTTCAG CAAGCTCGGG 
TTATCGCGAT CATGCAATCA GCCCTCACAG AGGAGCTGGA ATGAGCTGAG CAGCGGCCCC 
TCCTACCTCT GGGACTCGCC GGCCACAGAG CCCAGCCCTT CCTGGCCAGC CAGTTCCAGC 
TCCCCGACCC ACACGGCCAC ACCCATCCTT GGCAGCACCA GCAGCCTGTG GTCGTCCACT 
CCGTTCAGCA GCTCCATCTG GTCCAGCAGC CTGCACAGCA CCCTGCCCTT CACCAGCCCC 
ACCAGCGCGT TGCCGAGCAT CAGCCTCATG GGCCCCGAGA GCGCCCCGGC AGCTCACGCG 
CCCTCTGCCG CCAGCCCCTC CGACGACCTG GGCCAGACCT ACAACCCCTG GCGGATATGG 
AGTCCCACCA TCGGACGGAG GAGCTCAGAC CCCTGGTCGA ATTCGCACTT CCCTCACGAG 
AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005193867.1
CDS	558..6143
Translation	MGKRAGGGAAGSAAAASTSAVAGLEPAAGRGGGPRSAAAGLLGALHLVMTLVVAAARAEKEAFIQSESIIEVLRFDDGGLLQTETTLGLSSYQQKSISLYRGNCRPIRFEPPMLDFHEQPVGMPKMEKVYLHNPSSEETITLVSISATTSHFHASFFQNRKILPGGNTSFDVVFLARVVGNVENTLFINTSNHGVFTYQVFGVGVPNPYRLRPFLGARVPVNSSFSPIINIHNPHSEPLQVVEMYSSGGDLHLELPTGQQGGTRKLWEIPPYETKGVMRASFSSREADNHTAFIRIKTNASDSTEFIILPVEVEVTTAPGIYSSTEMLDFGTLRTQDLPKVLNLHLLNSGTKDVPITSVRPTPQNDAVTVHFKPITLKASESKYTKVASISFDASKAKKPSQFSGKITVKAKEKSYSKLEIPYQAEVLDGYLGFDHAATLFHIRDSPADPVERPIYLTNTFSFAILIHDVLLPEEAKTMFKVHNFSKPVLILPNGSGYIFTLFFMPSTSSMHIDNNILLITNASKFHLPVRVYTGFLDYFVLPPKIEERFIDFGILSATEASNILFAIINSNPIELAIKSWHIIGDGLSIELVATERGNRTTIISSLPEFEKSSLSDQSSVILASGYFAVFRVKLTAKKLEGIHDGAIQITTDYEILTIPVKAVIAVGSLTCFPKHMVLPPSFPGKIVHQSLNIMNSFSQKVKIQQIRSLSEDVRFYYKRLRGNKEDLEPGKKSKIANIYFDPGLQCGDHCYVGLPFLSKSEPRVQPGIAMQEDAWDTDWDLHQSLFKGWMGIKENSGHKLSAMFEVNTDLQKNILSKVSAELSWPSLLSSPRLVRFPLTNTNCSSEEEIMLENPADVPIYIQFIPLALYSSPSIFVDKLISRFNLSKVAKIDLRTQEFQVFRNTAHPLQSSTGFTEGLSRHLVLNLILKPGEKKSVKVKFTPVHNRTVSSLIIIRNNLTVMDAVMVQGQGTTENLRVAGKLPGPGSSLRFKITEALLKDCTEGMRLREPNFTLKRTFKVENTGQLPIHIETIEVSGYSCEGYGFKVVNCQEFALSANASKDIVILFTPDFTASRVIRELKFITASGSEFVFVLNASLPYHMLATCAEALPRPNWELALYIIISGIMSALFLLVIGTAYLEAQGIWEPFRRRLSFEASNPPFDVGRPFDLRRIVGISSEGNLNTLSCDPGHSRGFCGAGGSASRPGAGSHKQCSPSVHPHSSHSNRNSADVENVRAKNSASASGRTNAQSASKASPLVFESHSMAQGHTAGRKSKGARQGQHSSQHHGHSPLEQHSPPPPPPVPQQQEPQPERLSPAPLAPPPHPEHPGSTRHSSEDSDITSLIEAMDKDFEHHDAPPLDVFTEQPPSPLSKSKGKGKPPQRKVKPPKKQEEKEKKGKGRALEDELKDSLADDDSSSTTTETSNPDTEPLLKEDTEKQKGKQAMPEKHENEMSQVKQKSKKVLNVKKEIPADVKPSCLELPYTAPLESKQRRNLPTKIPLPTTLTSGSKPRNSQKTKGTSKLVDSRPPALAKFLPNSQELGNTSSSEGEKDSPPPEWDSVPVHKPGSYIFLKQRQTSPPPTSPSPPAAPCPFTSRGSYSSIVHGSPGSDSKAKQPSGSKHKLTKAASLPGKNGNPTFAAVTAGYDKSPGGNGFAKVSSSKTDFSSSLGISHTPVDSDGSDSSGLWSPVSNPSSPDFTPLNSFSAFGNSFNLTGEVFSKLGLSRSCNQPSQRSWNELSSGPSYLWDSPATEPSPSWPASSSSPTHTATPILGSTSSLWSSTPFSSSIWSSSLHSTLPFTSPTSALPSISLMGPESAPAAHAPSAASPSDDLGQTYNPWRIWSPTIGRRSSDPWSNSHFPHEN

Target ORF information:

RefSeq Version	XM_005193810.3
Organism	Bos taurus(cattle)
Definition	Bos taurus transmembrane protein 131 (TMEM131), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005193810.3

ORF Insert Sequence:

ATGGGGAAGC GGGCCGGAGG AGGAGCAGCG GGATCCGCCG CCGCTGCCTC CACGTCCGCC 
GTGGCCGGTC TGGAGCCCGC GGCCGGCCGC GGCGGGGGCC CGCGGAGCGC AGCCGCCGGC 
CTCCTGGGAG CGCTGCACCT GGTGATGACC CTCGTCGTGG CCGCGGCGCG GGCCGAGAAG 
GAAGCATTTA TTCAGTCGGA GAGCATAATA GAAGTGCTGC GTTTTGATGA TGGAGGGCTT 
CTACAGACTG AGACAACACT TGGACTCAGT TCATACCAAC AGAAAAGTAT ATCTTTGTAC 
CGGGGTAATT GCAGACCTAT ACGGTTTGAG CCACCAATGC TGGATTTCCA TGAACAACCA 
GTTGGAATGC CAAAAATGGA AAAAGTTTAC TTACATAATC CTAGTTCTGA AGAAACTATT 
ACTTTAGTAT CAATATCTGC TACCACATCA CATTTTCACG CATCATTTTT TCAAAATAGG 
AAAATTCTTC CAGGAGGAAA TACATCATTT GATGTAGTTT TTCTTGCAAG AGTAGTGGGA 
AATGTAGAAA ATACTTTATT TATTAATACA TCTAATCATG GGGTATTCAC TTACCAGGTA 
TTTGGTGTTG GAGTTCCAAA TCCATACCGA TTGAGGCCAT TCCTTGGGGC CAGAGTCCCT 
GTGAATAGCA GTTTCTCACC CATAATAAAC ATACACAATC CTCACAGCGA GCCTTTACAG 
GTTGTAGAAA TGTACTCTAG TGGAGGAGAC CTTCACCTTG AACTTCCCAC AGGGCAGCAG 
GGAGGCACCA GGAAGCTGTG GGAAATTCCT CCTTATGAAA CCAAGGGAGT GATGAGAGCC 
AGTTTTTCAT CTAGAGAAGC AGATAATCAC ACAGCTTTCA TAAGAATAAA GACAAATGCT 
TCAGACAGCA CGGAGTTTAT CATCCTTCCG GTTGAAGTGG AAGTCACAAC AGCTCCTGGA 
ATTTATTCCT CAACTGAAAT GTTAGATTTT GGCACACTGC GAACACAAGA TCTACCAAAA 
GTTTTAAATC TTCATTTATT AAATTCAGGA ACAAAAGATG TACCAATAAC AAGTGTTCGA 
CCTACACCAC AAAATGATGC TGTCACTGTA CACTTTAAAC CAATTACATT AAAAGCTTCA 
GAAAGTAAAT ATACCAAGGT AGCAAGTATT AGTTTTGATG CATCAAAGGC GAAAAAACCA 
TCTCAGTTTT CTGGGAAAAT AACTGTGAAA GCAAAGGAAA AGAGTTATTC TAAACTTGAA 
ATACCATATC AAGCAGAGGT TTTAGATGGT TATTTGGGAT TTGATCACGC TGCAACATTA 
TTTCACATTC GAGACAGTCC TGCTGATCCT GTGGAAAGGC CAATTTACCT TACTAACACT 
TTCAGCTTTG CGATCCTCAT TCATGATGTG TTGCTACCAG AAGAAGCCAA AACAATGTTT 
AAAGTTCACA ACTTCAGCAA ACCAGTCTTA ATTCTACCAA ATGGATCAGG CTACATTTTT 
ACTCTATTTT TTATGCCATC CACATCGTCC ATGCACATAG ATAACAATAT TTTACTTATT 
ACCAATGCCT CTAAGTTTCA CTTACCTGTG CGGGTATATA CAGGCTTTTT AGATTACTTT 
GTATTACCCC CCAAAATAGA AGAACGTTTC ATAGATTTTG GTATACTGAG TGCAACAGAA 
GCAAGTAATA TTTTATTTGC CATTATAAAC AGCAATCCAA TTGAGTTGGC TATAAAAAGC 
TGGCATATTA TAGGAGATGG TTTATCAATA GAACTCGTAG CTACTGAAAG AGGCAATAGA 
ACTACAATCA TCTCAAGCCT TCCAGAGTTT GAAAAATCCT CTCTATCAGA CCAGTCATCG 
GTAATACTAG CTTCAGGATA CTTTGCAGTG TTCAGAGTCA AACTTACTGC AAAAAAACTG 
GAAGGAATTC ATGATGGGGC TATACAGATC ACGACAGACT ATGAGATCCT AACAATTCCT 
GTGAAGGCTG TGATCGCAGT GGGTTCTTTG ACTTGCTTCC CAAAGCACAT GGTTCTTCCA 
CCTTCCTTTC CAGGGAAAAT AGTTCATCAG AGTTTAAATA TTATGAATTC CTTCTCACAG 
AAGGTAAAGA TCCAACAGAT ACGATCTTTG TCAGAAGACG TGCGTTTTTA CTACAAACGA 
TTGCGGGGTA ATAAGGAAGA CTTGGAGCCA GGGAAAAAAT CAAAGATTGC AAACATTTAC 
TTTGATCCTG GATTGCAGTG CGGGGATCAT TGCTATGTCG GCTTGCCTTT TCTGTCCAAA 
TCTGAACCCA GAGTCCAGCC TGGCATAGCC ATGCAGGAAG ATGCCTGGGA CACCGACTGG 
GATTTGCATC AAAGCCTATT CAAGGGATGG ATGGGAATAA AGGAAAACTC AGGTCACAAA 
TTGAGCGCTA TGTTTGAAGT GAATACAGAC CTTCAGAAAA ACATCCTGTC AAAAGTTTCT 
GCCGAGCTCT CCTGGCCATC CCTCCTCAGC TCCCCCCGGC TCGTGAGGTT TCCCCTCACT 
AATACAAACT GCTCTTCAGA AGAAGAGATT ATGTTAGAAA ACCCTGCAGA TGTTCCCATC 
TATATTCAAT TTATCCCCCT GGCTTTATAT TCCAGCCCTT CCATCTTTGT AGATAAGTTA 
ATATCAAGGT TTAACTTAAG TAAGGTGGCA AAGATCGATT TAAGAACACA AGAATTCCAA 
GTCTTTAGAA ACACTGCTCA CCCACTGCAG AGTTCAACAG GATTCACAGA GGGCCTCTCC 
CGACATTTAG TTTTAAACTT AATTTTAAAA CCTGGAGAAA AGAAATCTGT CAAAGTAAAG 
TTTACTCCAG TTCACAATAG AACTGTTTCT TCCCTAATCA TAATCAGAAA TAACCTGACT 
GTGATGGATG CCGTAATGGT CCAAGGACAG GGGACAACCG AGAACCTGAG GGTGGCAGGC 
AAGCTTCCGG GCCCAGGGAG CTCCTTACGC TTCAAAATCA CAGAAGCACT GCTGAAAGAC 
TGTACGGAGG GTATGAGACT AAGGGAACCC AATTTCACAT TGAAAAGAAC ATTTAAAGTA 
GAGAACACAG GACAACTTCC AATTCACATA GAAACCATTG AAGTCAGTGG GTACTCTTGT 
GAGGGATATG GCTTTAAAGT CGTCAATTGT CAAGAGTTTG CACTGAGCGC CAATGCCTCT 
AAAGACATAG TCATTCTGTT TACTCCTGAT TTCACGGCTT CTAGAGTCAT CCGTGAACTG 
AAGTTTATAA CAGCCAGTGG CTCTGAGTTT GTGTTTGTAT TGAATGCATC CCTTCCTTAC 
CATATGTTAG CAACCTGTGC AGAAGCCCTG CCCAGGCCCA ACTGGGAGCT GGCTCTGTAC 
ATCATCATCT CAGGAATAAT GAGTGCGCTG TTTCTCTTGG TAATTGGAAC AGCCTATTTA 
GAAGCTCAAG GAATTTGGGA GCCCTTCCGA AGGCGGCTGT CCTTTGAGGC CTCGAACCCG 
CCCTTTGATG TGGGGAGACC ATTTGATCTC AGGAGAATCG TTGGTATTTC ATCTGAAGGA 
AACTTGAACA CACTCAGCTG TGACCCCGGT CACAGTCGGG GCTTCTGTGG AGCAGGTGGC 
TCGGCCTCCC GGCCCGGCGC AGGGAGTCAC AAGCAGTGTA GCCCGTCGGT CCACCCGCAC 
AGCAGTCACA GCAATAGAAA CTCGGCTGAC GTGGAAAACG TCAGAGCCAA AAACAGTGCG 
AGTGCCTCTG GCAGGACTAA TGCCCAGTCG GCGAGCAAAG CCAGCCCACT GGTCTTCGAG 
TCCCACAGCA TGGCCCAGGG TCACACCGCA GGCCGGAAGT CCAAGGGGGC CAGACAGGGC 
CAGCACAGCA GCCAGCACCA TGGCCACAGC CCCCTGGAAC AGCACTCGCC CCCGCCGCCG 
CCGCCAGTGC CTCAGCAGCA GGAGCCACAG CCCGAGAGGC TGTCCCCTGC GCCCCTCGCA 
CCCCCGCCCC ACCCAGAGCA CCCCGGCAGC ACCCGGCACA GCTCCGAGGA CTCAGACATC 
ACCAGTCTCA TCGAAGCCAT GGACAAAGAC TTCGAGCACC ACGACGCCCC GCCCCTAGAT 
GTGTTTACAG AGCAGCCGCC TTCACCCTTG TCGAAAAGCA AAGGGAAAGG AAAGCCTCCT 
CAGCGCAAGG TGAAGCCCCC CAAGAAGCAG GAGGAGAAGG AGAAGAAGGG AAAGGGGAGG 
GCCCTGGAAG ACGAGCTGAA GGACTCCTTG GCTGACGACG ACAGCTCCTC CACCACCACA 
GAGACCTCCA ACCCAGACAC AGAGCCTCTC CTCAAGGAGG ATACAGAAAA GCAAAAGGGA 
AAACAAGCCA TGCCTGAAAA ACATGAAAAC GAAATGTCTC AAGTGAAACA AAAAAGCAAA 
AAAGTCTTAA ATGTTAAGAA AGAAATCCCA GCAGATGTGA AACCCAGTTG CTTAGAGCTA 
CCGTATACTG CCCCCTTGGA AAGCAAACAG CGCCGAAATC TCCCAACCAA GATTCCTCTT 
CCAACTACAC TGACCAGTGG ATCCAAACCA CGAAACTCCC AGAAAACAAA AGGTACAAGT 
AAGTTAGTGG ATAGCAGGCC ACCTGCCCTC GCAAAGTTTC TCCCAAACAG TCAAGAACTA 
GGCAACACCA GCAGCTCAGA AGGTGAAAAA GACTCTCCAC CACCAGAGTG GGATTCCGTG 
CCTGTTCACA AGCCCGGCAG CTACATCTTC CTGAAACAAC GCCAGACCTC GCCGCCGCCC 
ACCTCCCCCT CACCGCCCGC CGCACCCTGC CCGTTCACGT CCCGGGGCAG CTACAGCAGC 
ATCGTCCATG GCAGCCCGGG CAGCGACTCT AAAGCAAAGC AGCCAAGCGG GAGCAAACAC 
AAACTGACAA AAGCCGCTTC TCTCCCGGGC AAGAATGGCA ACCCCACTTT CGCTGCGGTC 
ACGGCTGGGT ATGACAAGAG CCCAGGTGGG AACGGCTTTG CCAAAGTTTC TTCAAGCAAA 
ACAGATTTCT CTAGCAGCCT TGGCATTTCA CACACTCCTG TTGACAGCGA TGGCTCAGAC 
AGCTCGGGTC TGTGGAGCCC CGTGAGCAAC CCCAGCAGCC CTGACTTCAC ACCCCTCAAC 
TCGTTCTCAG CCTTTGGGAA TTCTTTTAAC CTAACTGGTG AAGTGTTCAG CAAGCTCGGG 
TTATCGCGAT CATGCAATCA GCCCTCACAG AGGAGCTGGA ATGAGCTGAG CAGCGGCCCC 
TCCTACCTCT GGGACTCGCC GGCCACAGAG CCCAGCCCTT CCTGGCCAGC CAGTTCCAGC 
TCCCCGACCC ACACGGCCAC ACCCATCCTT GGCAGCACCA GCAGCCTGTG GTCGTCCACT 
CCGTTCAGCA GCTCCATCTG GTCCAGCAGC CTGCACAGCA CCCTGCCCTT CACCAGCCCC 
ACCAGCGCGT TGCCGAGCAT CAGCCTCATG GGCCCCGAGA GCGCCCCGGC AGCTCACGCG 
CCCTCTGCCG CCAGCCCCTC CGACGACCTG GGCCAGACCT ACAACCCCTG GCGGATATGG 
AGTCCCACCA TCGGACGGAG GAGCTCAGAC CCCTGGTCGA ATTCGCACTT CCCTCACGAG 
AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM131 ( XM_005212403.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212460.1 Bos taurus transmembrane protein 131 (TMEM131), transcript variant X2, mRNA.

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CloneID	OBa06772
Clone ID Related Accession (Same CDS sequence)	XM_005193810.3 , XM_005212403.3 , XM_005212403.4
Accession Version	XM_005212403.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transmembrane protein 131 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212403.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGC GGGCCGGAGG AGGAGCAGCG GGATCCGCCG CCGCTGCCTC CACGTCCGCC 
GTGGCCGGTC TGGAGCCCGC GGCCGGCCGC GGCGGGGGCC CGCGGAGCGC AGCCGCCGGC 
CTCCTGGGAG CGCTGCACCT GGTGATGACC CTCGTCGTGG CCGCGGCGCG GGCCGAGAAG 
GAAGCATTTA TTCAGTCGGA GAGCATAATA GAAGTGCTGC GTTTTGATGA TGGAGGGCTT 
CTACAGACTG AGACAACACT TGGACTCAGT TCATACCAAC AGAAAAGTAT ATCTTTGTAC 
CGGGGTAATT GCAGACCTAT ACGGTTTGAG CCACCAATGC TGGATTTCCA TGAACAACCA 
GTTGGAATGC CAAAAATGGA AAAAGTTTAC TTACATAATC CTAGTTCTGA AGAAACTATT 
ACTTTAGTAT CAATATCTGC TACCACATCA CATTTTCACG CATCATTTTT TCAAAATAGG 
AAAATTCTTC CAGGAGGAAA TACATCATTT GATGTAGTTT TTCTTGCAAG AGTAGTGGGA 
AATGTAGAAA ATACTTTATT TATTAATACA TCTAATCATG GGGTATTCAC TTACCAGGTA 
TTTGGTGTTG GAGTTCCAAA TCCATACCGA TTGAGGCCAT TCCTTGGGGC CAGAGTCCCT 
GTGAATAGCA GTTTCTCACC CATAATAAAC ATACACAATC CTCACAGCGA GCCTTTACAG 
GTTGTAGAAA TGTACTCTAG TGGAGGAGAC CTTCACCTTG AACTTCCCAC AGGGCAGCAG 
GGAGGCACCA GGAAGCTGTG GGAAATTCCT CCTTATGAAA CCAAGGGAGT GATGAGAGCC 
AGTTTTTCAT CTAGAGAAGC AGATAATCAC ACAGCTTTCA TAAGAATAAA GACAAATGCT 
TCAGACAGCA CGGAGTTTAT CATCCTTCCG GTTGAAGTGG AAGTCACAAC AGCTCCTGGA 
ATTTATTCCT CAACTGAAAT GTTAGATTTT GGCACACTGC GAACACAAGA TCTACCAAAA 
GTTTTAAATC TTCATTTATT AAATTCAGGA ACAAAAGATG TACCAATAAC AAGTGTTCGA 
CCTACACCAC AAAATGATGC TGTCACTGTA CACTTTAAAC CAATTACATT AAAAGCTTCA 
GAAAGTAAAT ATACCAAGGT AGCAAGTATT AGTTTTGATG CATCAAAGGC GAAAAAACCA 
TCTCAGTTTT CTGGGAAAAT AACTGTGAAA GCAAAGGAAA AGAGTTATTC TAAACTTGAA 
ATACCATATC AAGCAGAGGT TTTAGATGGT TATTTGGGAT TTGATCACGC TGCAACATTA 
TTTCACATTC GAGACAGTCC TGCTGATCCT GTGGAAAGGC CAATTTACCT TACTAACACT 
TTCAGCTTTG CGATCCTCAT TCATGATGTG TTGCTACCAG AAGAAGCCAA AACAATGTTT 
AAAGTTCACA ACTTCAGCAA ACCAGTCTTA ATTCTACCAA ATGGATCAGG CTACATTTTT 
ACTCTATTTT TTATGCCATC CACATCGTCC ATGCACATAG ATAACAATAT TTTACTTATT 
ACCAATGCCT CTAAGTTTCA CTTACCTGTG CGGGTATATA CAGGCTTTTT AGATTACTTT 
GTATTACCCC CCAAAATAGA AGAACGTTTC ATAGATTTTG GTATACTGAG TGCAACAGAA 
GCAAGTAATA TTTTATTTGC CATTATAAAC AGCAATCCAA TTGAGTTGGC TATAAAAAGC 
TGGCATATTA TAGGAGATGG TTTATCAATA GAACTCGTAG CTACTGAAAG AGGCAATAGA 
ACTACAATCA TCTCAAGCCT TCCAGAGTTT GAAAAATCCT CTCTATCAGA CCAGTCATCG 
GTAATACTAG CTTCAGGATA CTTTGCAGTG TTCAGAGTCA AACTTACTGC AAAAAAACTG 
GAAGGAATTC ATGATGGGGC TATACAGATC ACGACAGACT ATGAGATCCT AACAATTCCT 
GTGAAGGCTG TGATCGCAGT GGGTTCTTTG ACTTGCTTCC CAAAGCACAT GGTTCTTCCA 
CCTTCCTTTC CAGGGAAAAT AGTTCATCAG AGTTTAAATA TTATGAATTC CTTCTCACAG 
AAGGTAAAGA TCCAACAGAT ACGATCTTTG TCAGAAGACG TGCGTTTTTA CTACAAACGA 
TTGCGGGGTA ATAAGGAAGA CTTGGAGCCA GGGAAAAAAT CAAAGATTGC AAACATTTAC 
TTTGATCCTG GATTGCAGTG CGGGGATCAT TGCTATGTCG GCTTGCCTTT TCTGTCCAAA 
TCTGAACCCA GAGTCCAGCC TGGCATAGCC ATGCAGGAAG ATGCCTGGGA CACCGACTGG 
GATTTGCATC AAAGCCTATT CAAGGGATGG ATGGGAATAA AGGAAAACTC AGGTCACAAA 
TTGAGCGCTA TGTTTGAAGT GAATACAGAC CTTCAGAAAA ACATCCTGTC AAAAGTTTCT 
GCCGAGCTCT CCTGGCCATC CCTCCTCAGC TCCCCCCGGC TCGTGAGGTT TCCCCTCACT 
AATACAAACT GCTCTTCAGA AGAAGAGATT ATGTTAGAAA ACCCTGCAGA TGTTCCCATC 
TATATTCAAT TTATCCCCCT GGCTTTATAT TCCAGCCCTT CCATCTTTGT AGATAAGTTA 
ATATCAAGGT TTAACTTAAG TAAGGTGGCA AAGATCGATT TAAGAACACA AGAATTCCAA 
GTCTTTAGAA ACACTGCTCA CCCACTGCAG AGTTCAACAG GATTCACAGA GGGCCTCTCC 
CGACATTTAG TTTTAAACTT AATTTTAAAA CCTGGAGAAA AGAAATCTGT CAAAGTAAAG 
TTTACTCCAG TTCACAATAG AACTGTTTCT TCCCTAATCA TAATCAGAAA TAACCTGACT 
GTGATGGATG CCGTAATGGT CCAAGGACAG GGGACAACCG AGAACCTGAG GGTGGCAGGC 
AAGCTTCCGG GCCCAGGGAG CTCCTTACGC TTCAAAATCA CAGAAGCACT GCTGAAAGAC 
TGTACGGAGG GTATGAGACT AAGGGAACCC AATTTCACAT TGAAAAGAAC ATTTAAAGTA 
GAGAACACAG GACAACTTCC AATTCACATA GAAACCATTG AAGTCAGTGG GTACTCTTGT 
GAGGGATATG GCTTTAAAGT CGTCAATTGT CAAGAGTTTG CACTGAGCGC CAATGCCTCT 
AAAGACATAG TCATTCTGTT TACTCCTGAT TTCACGGCTT CTAGAGTCAT CCGTGAACTG 
AAGTTTATAA CAGCCAGTGG CTCTGAGTTT GTGTTTGTAT TGAATGCATC CCTTCCTTAC 
CATATGTTAG CAACCTGTGC AGAAGCCCTG CCCAGGCCCA ACTGGGAGCT GGCTCTGTAC 
ATCATCATCT CAGGAATAAT GAGTGCGCTG TTTCTCTTGG TAATTGGAAC AGCCTATTTA 
GAAGCTCAAG GAATTTGGGA GCCCTTCCGA AGGCGGCTGT CCTTTGAGGC CTCGAACCCG 
CCCTTTGATG TGGGGAGACC ATTTGATCTC AGGAGAATCG TTGGTATTTC ATCTGAAGGA 
AACTTGAACA CACTCAGCTG TGACCCCGGT CACAGTCGGG GCTTCTGTGG AGCAGGTGGC 
TCGGCCTCCC GGCCCGGCGC AGGGAGTCAC AAGCAGTGTA GCCCGTCGGT CCACCCGCAC 
AGCAGTCACA GCAATAGAAA CTCGGCTGAC GTGGAAAACG TCAGAGCCAA AAACAGTGCG 
AGTGCCTCTG GCAGGACTAA TGCCCAGTCG GCGAGCAAAG CCAGCCCACT GGTCTTCGAG 
TCCCACAGCA TGGCCCAGGG TCACACCGCA GGCCGGAAGT CCAAGGGGGC CAGACAGGGC 
CAGCACAGCA GCCAGCACCA TGGCCACAGC CCCCTGGAAC AGCACTCGCC CCCGCCGCCG 
CCGCCAGTGC CTCAGCAGCA GGAGCCACAG CCCGAGAGGC TGTCCCCTGC GCCCCTCGCA 
CCCCCGCCCC ACCCAGAGCA CCCCGGCAGC ACCCGGCACA GCTCCGAGGA CTCAGACATC 
ACCAGTCTCA TCGAAGCCAT GGACAAAGAC TTCGAGCACC ACGACGCCCC GCCCCTAGAT 
GTGTTTACAG AGCAGCCGCC TTCACCCTTG TCGAAAAGCA AAGGGAAAGG AAAGCCTCCT 
CAGCGCAAGG TGAAGCCCCC CAAGAAGCAG GAGGAGAAGG AGAAGAAGGG AAAGGGGAGG 
GCCCTGGAAG ACGAGCTGAA GGACTCCTTG GCTGACGACG ACAGCTCCTC CACCACCACA 
GAGACCTCCA ACCCAGACAC AGAGCCTCTC CTCAAGGAGG ATACAGAAAA GCAAAAGGGA 
AAACAAGCCA TGCCTGAAAA ACATGAAAAC GAAATGTCTC AAGTGAAACA AAAAAGCAAA 
AAAGTCTTAA ATGTTAAGAA AGAAATCCCA GCAGATGTGA AACCCAGTTG CTTAGAGCTA 
CCGTATACTG CCCCCTTGGA AAGCAAACAG CGCCGAAATC TCCCAACCAA GATTCCTCTT 
CCAACTACAC TGACCAGTGG ATCCAAACCA CGAAACTCCC AGAAAACAAA AGGTACAAGT 
AAGTTAGTGG ATAGCAGGCC ACCTGCCCTC GCAAAGTTTC TCCCAAACAG TCAAGAACTA 
GGCAACACCA GCAGCTCAGA AGGTGAAAAA GACTCTCCAC CACCAGAGTG GGATTCCGTG 
CCTGTTCACA AGCCCGGCAG CTACATCTTC CTGAAACAAC GCCAGACCTC GCCGCCGCCC 
ACCTCCCCCT CACCGCCCGC CGCACCCTGC CCGTTCACGT CCCGGGGCAG CTACAGCAGC 
ATCGTCCATG GCAGCCCGGG CAGCGACTCT AAAGCAAAGC AGCCAAGCGG GAGCAAACAC 
AAACTGACAA AAGCCGCTTC TCTCCCGGGC AAGAATGGCA ACCCCACTTT CGCTGCGGTC 
ACGGCTGGGT ATGACAAGAG CCCAGGTGGG AACGGCTTTG CCAAAGTTTC TTCAAGCAAA 
ACAGATTTCT CTAGCAGCCT TGGCATTTCA CACACTCCTG TTGACAGCGA TGGCTCAGAC 
AGCTCGGGTC TGTGGAGCCC CGTGAGCAAC CCCAGCAGCC CTGACTTCAC ACCCCTCAAC 
TCGTTCTCAG CCTTTGGGAA TTCTTTTAAC CTAACTGGTG AAGTGTTCAG CAAGCTCGGG 
TTATCGCGAT CATGCAATCA GCCCTCACAG AGGAGCTGGA ATGAGCTGAG CAGCGGCCCC 
TCCTACCTCT GGGACTCGCC GGCCACAGAG CCCAGCCCTT CCTGGCCAGC CAGTTCCAGC 
TCCCCGACCC ACACGGCCAC ACCCATCCTT GGCAGCACCA GCAGCCTGTG GTCGTCCACT 
CCGTTCAGCA GCTCCATCTG GTCCAGCAGC CTGCACAGCA CCCTGCCCTT CACCAGCCCC 
ACCAGCGCGT TGCCGAGCAT CAGCCTCATG GGCCCCGAGA GCGCCCCGGC AGCTCACGCG 
CCCTCTGCCG CCAGCCCCTC CGACGACCTG GGCCAGACCT ACAACCCCTG GCGGATATGG 
AGTCCCACCA TCGGACGGAG GAGCTCAGAC CCCTGGTCGA ATTCGCACTT CCCTCACGAG 
AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212460.1
CDS	558..6143
Translation	MGKRAGGGAAGSAAAASTSAVAGLEPAAGRGGGPRSAAAGLLGALHLVMTLVVAAARAEKEAFIQSESIIEVLRFDDGGLLQTETTLGLSSYQQKSISLYRGNCRPIRFEPPMLDFHEQPVGMPKMEKVYLHNPSSEETITLVSISATTSHFHASFFQNRKILPGGNTSFDVVFLARVVGNVENTLFINTSNHGVFTYQVFGVGVPNPYRLRPFLGARVPVNSSFSPIINIHNPHSEPLQVVEMYSSGGDLHLELPTGQQGGTRKLWEIPPYETKGVMRASFSSREADNHTAFIRIKTNASDSTEFIILPVEVEVTTAPGIYSSTEMLDFGTLRTQDLPKVLNLHLLNSGTKDVPITSVRPTPQNDAVTVHFKPITLKASESKYTKVASISFDASKAKKPSQFSGKITVKAKEKSYSKLEIPYQAEVLDGYLGFDHAATLFHIRDSPADPVERPIYLTNTFSFAILIHDVLLPEEAKTMFKVHNFSKPVLILPNGSGYIFTLFFMPSTSSMHIDNNILLITNASKFHLPVRVYTGFLDYFVLPPKIEERFIDFGILSATEASNILFAIINSNPIELAIKSWHIIGDGLSIELVATERGNRTTIISSLPEFEKSSLSDQSSVILASGYFAVFRVKLTAKKLEGIHDGAIQITTDYEILTIPVKAVIAVGSLTCFPKHMVLPPSFPGKIVHQSLNIMNSFSQKVKIQQIRSLSEDVRFYYKRLRGNKEDLEPGKKSKIANIYFDPGLQCGDHCYVGLPFLSKSEPRVQPGIAMQEDAWDTDWDLHQSLFKGWMGIKENSGHKLSAMFEVNTDLQKNILSKVSAELSWPSLLSSPRLVRFPLTNTNCSSEEEIMLENPADVPIYIQFIPLALYSSPSIFVDKLISRFNLSKVAKIDLRTQEFQVFRNTAHPLQSSTGFTEGLSRHLVLNLILKPGEKKSVKVKFTPVHNRTVSSLIIIRNNLTVMDAVMVQGQGTTENLRVAGKLPGPGSSLRFKITEALLKDCTEGMRLREPNFTLKRTFKVENTGQLPIHIETIEVSGYSCEGYGFKVVNCQEFALSANASKDIVILFTPDFTASRVIRELKFITASGSEFVFVLNASLPYHMLATCAEALPRPNWELALYIIISGIMSALFLLVIGTAYLEAQGIWEPFRRRLSFEASNPPFDVGRPFDLRRIVGISSEGNLNTLSCDPGHSRGFCGAGGSASRPGAGSHKQCSPSVHPHSSHSNRNSADVENVRAKNSASASGRTNAQSASKASPLVFESHSMAQGHTAGRKSKGARQGQHSSQHHGHSPLEQHSPPPPPPVPQQQEPQPERLSPAPLAPPPHPEHPGSTRHSSEDSDITSLIEAMDKDFEHHDAPPLDVFTEQPPSPLSKSKGKGKPPQRKVKPPKKQEEKEKKGKGRALEDELKDSLADDDSSSTTTETSNPDTEPLLKEDTEKQKGKQAMPEKHENEMSQVKQKSKKVLNVKKEIPADVKPSCLELPYTAPLESKQRRNLPTKIPLPTTLTSGSKPRNSQKTKGTSKLVDSRPPALAKFLPNSQELGNTSSSEGEKDSPPPEWDSVPVHKPGSYIFLKQRQTSPPPTSPSPPAAPCPFTSRGSYSSIVHGSPGSDSKAKQPSGSKHKLTKAASLPGKNGNPTFAAVTAGYDKSPGGNGFAKVSSSKTDFSSSLGISHTPVDSDGSDSSGLWSPVSNPSSPDFTPLNSFSAFGNSFNLTGEVFSKLGLSRSCNQPSQRSWNELSSGPSYLWDSPATEPSPSWPASSSSPTHTATPILGSTSSLWSSTPFSSSIWSSSLHSTLPFTSPTSALPSISLMGPESAPAAHAPSAASPSDDLGQTYNPWRIWSPTIGRRSSDPWSNSHFPHEN

Target ORF information:

RefSeq Version	XM_005212403.3
Organism	Bos taurus(cattle)
Definition	Bos taurus transmembrane protein 131 (TMEM131), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212403.3

ORF Insert Sequence:

ATGGGGAAGC GGGCCGGAGG AGGAGCAGCG GGATCCGCCG CCGCTGCCTC CACGTCCGCC 
GTGGCCGGTC TGGAGCCCGC GGCCGGCCGC GGCGGGGGCC CGCGGAGCGC AGCCGCCGGC 
CTCCTGGGAG CGCTGCACCT GGTGATGACC CTCGTCGTGG CCGCGGCGCG GGCCGAGAAG 
GAAGCATTTA TTCAGTCGGA GAGCATAATA GAAGTGCTGC GTTTTGATGA TGGAGGGCTT 
CTACAGACTG AGACAACACT TGGACTCAGT TCATACCAAC AGAAAAGTAT ATCTTTGTAC 
CGGGGTAATT GCAGACCTAT ACGGTTTGAG CCACCAATGC TGGATTTCCA TGAACAACCA 
GTTGGAATGC CAAAAATGGA AAAAGTTTAC TTACATAATC CTAGTTCTGA AGAAACTATT 
ACTTTAGTAT CAATATCTGC TACCACATCA CATTTTCACG CATCATTTTT TCAAAATAGG 
AAAATTCTTC CAGGAGGAAA TACATCATTT GATGTAGTTT TTCTTGCAAG AGTAGTGGGA 
AATGTAGAAA ATACTTTATT TATTAATACA TCTAATCATG GGGTATTCAC TTACCAGGTA 
TTTGGTGTTG GAGTTCCAAA TCCATACCGA TTGAGGCCAT TCCTTGGGGC CAGAGTCCCT 
GTGAATAGCA GTTTCTCACC CATAATAAAC ATACACAATC CTCACAGCGA GCCTTTACAG 
GTTGTAGAAA TGTACTCTAG TGGAGGAGAC CTTCACCTTG AACTTCCCAC AGGGCAGCAG 
GGAGGCACCA GGAAGCTGTG GGAAATTCCT CCTTATGAAA CCAAGGGAGT GATGAGAGCC 
AGTTTTTCAT CTAGAGAAGC AGATAATCAC ACAGCTTTCA TAAGAATAAA GACAAATGCT 
TCAGACAGCA CGGAGTTTAT CATCCTTCCG GTTGAAGTGG AAGTCACAAC AGCTCCTGGA 
ATTTATTCCT CAACTGAAAT GTTAGATTTT GGCACACTGC GAACACAAGA TCTACCAAAA 
GTTTTAAATC TTCATTTATT AAATTCAGGA ACAAAAGATG TACCAATAAC AAGTGTTCGA 
CCTACACCAC AAAATGATGC TGTCACTGTA CACTTTAAAC CAATTACATT AAAAGCTTCA 
GAAAGTAAAT ATACCAAGGT AGCAAGTATT AGTTTTGATG CATCAAAGGC GAAAAAACCA 
TCTCAGTTTT CTGGGAAAAT AACTGTGAAA GCAAAGGAAA AGAGTTATTC TAAACTTGAA 
ATACCATATC AAGCAGAGGT TTTAGATGGT TATTTGGGAT TTGATCACGC TGCAACATTA 
TTTCACATTC GAGACAGTCC TGCTGATCCT GTGGAAAGGC CAATTTACCT TACTAACACT 
TTCAGCTTTG CGATCCTCAT TCATGATGTG TTGCTACCAG AAGAAGCCAA AACAATGTTT 
AAAGTTCACA ACTTCAGCAA ACCAGTCTTA ATTCTACCAA ATGGATCAGG CTACATTTTT 
ACTCTATTTT TTATGCCATC CACATCGTCC ATGCACATAG ATAACAATAT TTTACTTATT 
ACCAATGCCT CTAAGTTTCA CTTACCTGTG CGGGTATATA CAGGCTTTTT AGATTACTTT 
GTATTACCCC CCAAAATAGA AGAACGTTTC ATAGATTTTG GTATACTGAG TGCAACAGAA 
GCAAGTAATA TTTTATTTGC CATTATAAAC AGCAATCCAA TTGAGTTGGC TATAAAAAGC 
TGGCATATTA TAGGAGATGG TTTATCAATA GAACTCGTAG CTACTGAAAG AGGCAATAGA 
ACTACAATCA TCTCAAGCCT TCCAGAGTTT GAAAAATCCT CTCTATCAGA CCAGTCATCG 
GTAATACTAG CTTCAGGATA CTTTGCAGTG TTCAGAGTCA AACTTACTGC AAAAAAACTG 
GAAGGAATTC ATGATGGGGC TATACAGATC ACGACAGACT ATGAGATCCT AACAATTCCT 
GTGAAGGCTG TGATCGCAGT GGGTTCTTTG ACTTGCTTCC CAAAGCACAT GGTTCTTCCA 
CCTTCCTTTC CAGGGAAAAT AGTTCATCAG AGTTTAAATA TTATGAATTC CTTCTCACAG 
AAGGTAAAGA TCCAACAGAT ACGATCTTTG TCAGAAGACG TGCGTTTTTA CTACAAACGA 
TTGCGGGGTA ATAAGGAAGA CTTGGAGCCA GGGAAAAAAT CAAAGATTGC AAACATTTAC 
TTTGATCCTG GATTGCAGTG CGGGGATCAT TGCTATGTCG GCTTGCCTTT TCTGTCCAAA 
TCTGAACCCA GAGTCCAGCC TGGCATAGCC ATGCAGGAAG ATGCCTGGGA CACCGACTGG 
GATTTGCATC AAAGCCTATT CAAGGGATGG ATGGGAATAA AGGAAAACTC AGGTCACAAA 
TTGAGCGCTA TGTTTGAAGT GAATACAGAC CTTCAGAAAA ACATCCTGTC AAAAGTTTCT 
GCCGAGCTCT CCTGGCCATC CCTCCTCAGC TCCCCCCGGC TCGTGAGGTT TCCCCTCACT 
AATACAAACT GCTCTTCAGA AGAAGAGATT ATGTTAGAAA ACCCTGCAGA TGTTCCCATC 
TATATTCAAT TTATCCCCCT GGCTTTATAT TCCAGCCCTT CCATCTTTGT AGATAAGTTA 
ATATCAAGGT TTAACTTAAG TAAGGTGGCA AAGATCGATT TAAGAACACA AGAATTCCAA 
GTCTTTAGAA ACACTGCTCA CCCACTGCAG AGTTCAACAG GATTCACAGA GGGCCTCTCC 
CGACATTTAG TTTTAAACTT AATTTTAAAA CCTGGAGAAA AGAAATCTGT CAAAGTAAAG 
TTTACTCCAG TTCACAATAG AACTGTTTCT TCCCTAATCA TAATCAGAAA TAACCTGACT 
GTGATGGATG CCGTAATGGT CCAAGGACAG GGGACAACCG AGAACCTGAG GGTGGCAGGC 
AAGCTTCCGG GCCCAGGGAG CTCCTTACGC TTCAAAATCA CAGAAGCACT GCTGAAAGAC 
TGTACGGAGG GTATGAGACT AAGGGAACCC AATTTCACAT TGAAAAGAAC ATTTAAAGTA 
GAGAACACAG GACAACTTCC AATTCACATA GAAACCATTG AAGTCAGTGG GTACTCTTGT 
GAGGGATATG GCTTTAAAGT CGTCAATTGT CAAGAGTTTG CACTGAGCGC CAATGCCTCT 
AAAGACATAG TCATTCTGTT TACTCCTGAT TTCACGGCTT CTAGAGTCAT CCGTGAACTG 
AAGTTTATAA CAGCCAGTGG CTCTGAGTTT GTGTTTGTAT TGAATGCATC CCTTCCTTAC 
CATATGTTAG CAACCTGTGC AGAAGCCCTG CCCAGGCCCA ACTGGGAGCT GGCTCTGTAC 
ATCATCATCT CAGGAATAAT GAGTGCGCTG TTTCTCTTGG TAATTGGAAC AGCCTATTTA 
GAAGCTCAAG GAATTTGGGA GCCCTTCCGA AGGCGGCTGT CCTTTGAGGC CTCGAACCCG 
CCCTTTGATG TGGGGAGACC ATTTGATCTC AGGAGAATCG TTGGTATTTC ATCTGAAGGA 
AACTTGAACA CACTCAGCTG TGACCCCGGT CACAGTCGGG GCTTCTGTGG AGCAGGTGGC 
TCGGCCTCCC GGCCCGGCGC AGGGAGTCAC AAGCAGTGTA GCCCGTCGGT CCACCCGCAC 
AGCAGTCACA GCAATAGAAA CTCGGCTGAC GTGGAAAACG TCAGAGCCAA AAACAGTGCG 
AGTGCCTCTG GCAGGACTAA TGCCCAGTCG GCGAGCAAAG CCAGCCCACT GGTCTTCGAG 
TCCCACAGCA TGGCCCAGGG TCACACCGCA GGCCGGAAGT CCAAGGGGGC CAGACAGGGC 
CAGCACAGCA GCCAGCACCA TGGCCACAGC CCCCTGGAAC AGCACTCGCC CCCGCCGCCG 
CCGCCAGTGC CTCAGCAGCA GGAGCCACAG CCCGAGAGGC TGTCCCCTGC GCCCCTCGCA 
CCCCCGCCCC ACCCAGAGCA CCCCGGCAGC ACCCGGCACA GCTCCGAGGA CTCAGACATC 
ACCAGTCTCA TCGAAGCCAT GGACAAAGAC TTCGAGCACC ACGACGCCCC GCCCCTAGAT 
GTGTTTACAG AGCAGCCGCC TTCACCCTTG TCGAAAAGCA AAGGGAAAGG AAAGCCTCCT 
CAGCGCAAGG TGAAGCCCCC CAAGAAGCAG GAGGAGAAGG AGAAGAAGGG AAAGGGGAGG 
GCCCTGGAAG ACGAGCTGAA GGACTCCTTG GCTGACGACG ACAGCTCCTC CACCACCACA 
GAGACCTCCA ACCCAGACAC AGAGCCTCTC CTCAAGGAGG ATACAGAAAA GCAAAAGGGA 
AAACAAGCCA TGCCTGAAAA ACATGAAAAC GAAATGTCTC AAGTGAAACA AAAAAGCAAA 
AAAGTCTTAA ATGTTAAGAA AGAAATCCCA GCAGATGTGA AACCCAGTTG CTTAGAGCTA 
CCGTATACTG CCCCCTTGGA AAGCAAACAG CGCCGAAATC TCCCAACCAA GATTCCTCTT 
CCAACTACAC TGACCAGTGG ATCCAAACCA CGAAACTCCC AGAAAACAAA AGGTACAAGT 
AAGTTAGTGG ATAGCAGGCC ACCTGCCCTC GCAAAGTTTC TCCCAAACAG TCAAGAACTA 
GGCAACACCA GCAGCTCAGA AGGTGAAAAA GACTCTCCAC CACCAGAGTG GGATTCCGTG 
CCTGTTCACA AGCCCGGCAG CTACATCTTC CTGAAACAAC GCCAGACCTC GCCGCCGCCC 
ACCTCCCCCT CACCGCCCGC CGCACCCTGC CCGTTCACGT CCCGGGGCAG CTACAGCAGC 
ATCGTCCATG GCAGCCCGGG CAGCGACTCT AAAGCAAAGC AGCCAAGCGG GAGCAAACAC 
AAACTGACAA AAGCCGCTTC TCTCCCGGGC AAGAATGGCA ACCCCACTTT CGCTGCGGTC 
ACGGCTGGGT ATGACAAGAG CCCAGGTGGG AACGGCTTTG CCAAAGTTTC TTCAAGCAAA 
ACAGATTTCT CTAGCAGCCT TGGCATTTCA CACACTCCTG TTGACAGCGA TGGCTCAGAC 
AGCTCGGGTC TGTGGAGCCC CGTGAGCAAC CCCAGCAGCC CTGACTTCAC ACCCCTCAAC 
TCGTTCTCAG CCTTTGGGAA TTCTTTTAAC CTAACTGGTG AAGTGTTCAG CAAGCTCGGG 
TTATCGCGAT CATGCAATCA GCCCTCACAG AGGAGCTGGA ATGAGCTGAG CAGCGGCCCC 
TCCTACCTCT GGGACTCGCC GGCCACAGAG CCCAGCCCTT CCTGGCCAGC CAGTTCCAGC 
TCCCCGACCC ACACGGCCAC ACCCATCCTT GGCAGCACCA GCAGCCTGTG GTCGTCCACT 
CCGTTCAGCA GCTCCATCTG GTCCAGCAGC CTGCACAGCA CCCTGCCCTT CACCAGCCCC 
ACCAGCGCGT TGCCGAGCAT CAGCCTCATG GGCCCCGAGA GCGCCCCGGC AGCTCACGCG 
CCCTCTGCCG CCAGCCCCTC CGACGACCTG GGCCAGACCT ACAACCCCTG GCGGATATGG 
AGTCCCACCA TCGGACGGAG GAGCTCAGAC CCCTGGTCGA ATTCGCACTT CCCTCACGAG 
AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM131 ( XM_005212403.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212460.1 Bos taurus transmembrane protein 131 (TMEM131), transcript variant X2, mRNA.

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CloneID	OBa06772
Clone ID Related Accession (Same CDS sequence)	XM_005193810.3 , XM_005212403.3 , XM_005212403.4
Accession Version	XM_005212403.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transmembrane protein 131 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212403.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGC GGGCCGGAGG AGGAGCAGCG GGATCCGCCG CCGCTGCCTC CACGTCCGCC 
GTGGCCGGTC TGGAGCCCGC GGCCGGCCGC GGCGGGGGCC CGCGGAGCGC AGCCGCCGGC 
CTCCTGGGAG CGCTGCACCT GGTGATGACC CTCGTCGTGG CCGCGGCGCG GGCCGAGAAG 
GAAGCATTTA TTCAGTCGGA GAGCATAATA GAAGTGCTGC GTTTTGATGA TGGAGGGCTT 
CTACAGACTG AGACAACACT TGGACTCAGT TCATACCAAC AGAAAAGTAT ATCTTTGTAC 
CGGGGTAATT GCAGACCTAT ACGGTTTGAG CCACCAATGC TGGATTTCCA TGAACAACCA 
GTTGGAATGC CAAAAATGGA AAAAGTTTAC TTACATAATC CTAGTTCTGA AGAAACTATT 
ACTTTAGTAT CAATATCTGC TACCACATCA CATTTTCACG CATCATTTTT TCAAAATAGG 
AAAATTCTTC CAGGAGGAAA TACATCATTT GATGTAGTTT TTCTTGCAAG AGTAGTGGGA 
AATGTAGAAA ATACTTTATT TATTAATACA TCTAATCATG GGGTATTCAC TTACCAGGTA 
TTTGGTGTTG GAGTTCCAAA TCCATACCGA TTGAGGCCAT TCCTTGGGGC CAGAGTCCCT 
GTGAATAGCA GTTTCTCACC CATAATAAAC ATACACAATC CTCACAGCGA GCCTTTACAG 
GTTGTAGAAA TGTACTCTAG TGGAGGAGAC CTTCACCTTG AACTTCCCAC AGGGCAGCAG 
GGAGGCACCA GGAAGCTGTG GGAAATTCCT CCTTATGAAA CCAAGGGAGT GATGAGAGCC 
AGTTTTTCAT CTAGAGAAGC AGATAATCAC ACAGCTTTCA TAAGAATAAA GACAAATGCT 
TCAGACAGCA CGGAGTTTAT CATCCTTCCG GTTGAAGTGG AAGTCACAAC AGCTCCTGGA 
ATTTATTCCT CAACTGAAAT GTTAGATTTT GGCACACTGC GAACACAAGA TCTACCAAAA 
GTTTTAAATC TTCATTTATT AAATTCAGGA ACAAAAGATG TACCAATAAC AAGTGTTCGA 
CCTACACCAC AAAATGATGC TGTCACTGTA CACTTTAAAC CAATTACATT AAAAGCTTCA 
GAAAGTAAAT ATACCAAGGT AGCAAGTATT AGTTTTGATG CATCAAAGGC GAAAAAACCA 
TCTCAGTTTT CTGGGAAAAT AACTGTGAAA GCAAAGGAAA AGAGTTATTC TAAACTTGAA 
ATACCATATC AAGCAGAGGT TTTAGATGGT TATTTGGGAT TTGATCACGC TGCAACATTA 
TTTCACATTC GAGACAGTCC TGCTGATCCT GTGGAAAGGC CAATTTACCT TACTAACACT 
TTCAGCTTTG CGATCCTCAT TCATGATGTG TTGCTACCAG AAGAAGCCAA AACAATGTTT 
AAAGTTCACA ACTTCAGCAA ACCAGTCTTA ATTCTACCAA ATGGATCAGG CTACATTTTT 
ACTCTATTTT TTATGCCATC CACATCGTCC ATGCACATAG ATAACAATAT TTTACTTATT 
ACCAATGCCT CTAAGTTTCA CTTACCTGTG CGGGTATATA CAGGCTTTTT AGATTACTTT 
GTATTACCCC CCAAAATAGA AGAACGTTTC ATAGATTTTG GTATACTGAG TGCAACAGAA 
GCAAGTAATA TTTTATTTGC CATTATAAAC AGCAATCCAA TTGAGTTGGC TATAAAAAGC 
TGGCATATTA TAGGAGATGG TTTATCAATA GAACTCGTAG CTACTGAAAG AGGCAATAGA 
ACTACAATCA TCTCAAGCCT TCCAGAGTTT GAAAAATCCT CTCTATCAGA CCAGTCATCG 
GTAATACTAG CTTCAGGATA CTTTGCAGTG TTCAGAGTCA AACTTACTGC AAAAAAACTG 
GAAGGAATTC ATGATGGGGC TATACAGATC ACGACAGACT ATGAGATCCT AACAATTCCT 
GTGAAGGCTG TGATCGCAGT GGGTTCTTTG ACTTGCTTCC CAAAGCACAT GGTTCTTCCA 
CCTTCCTTTC CAGGGAAAAT AGTTCATCAG AGTTTAAATA TTATGAATTC CTTCTCACAG 
AAGGTAAAGA TCCAACAGAT ACGATCTTTG TCAGAAGACG TGCGTTTTTA CTACAAACGA 
TTGCGGGGTA ATAAGGAAGA CTTGGAGCCA GGGAAAAAAT CAAAGATTGC AAACATTTAC 
TTTGATCCTG GATTGCAGTG CGGGGATCAT TGCTATGTCG GCTTGCCTTT TCTGTCCAAA 
TCTGAACCCA GAGTCCAGCC TGGCATAGCC ATGCAGGAAG ATGCCTGGGA CACCGACTGG 
GATTTGCATC AAAGCCTATT CAAGGGATGG ATGGGAATAA AGGAAAACTC AGGTCACAAA 
TTGAGCGCTA TGTTTGAAGT GAATACAGAC CTTCAGAAAA ACATCCTGTC AAAAGTTTCT 
GCCGAGCTCT CCTGGCCATC CCTCCTCAGC TCCCCCCGGC TCGTGAGGTT TCCCCTCACT 
AATACAAACT GCTCTTCAGA AGAAGAGATT ATGTTAGAAA ACCCTGCAGA TGTTCCCATC 
TATATTCAAT TTATCCCCCT GGCTTTATAT TCCAGCCCTT CCATCTTTGT AGATAAGTTA 
ATATCAAGGT TTAACTTAAG TAAGGTGGCA AAGATCGATT TAAGAACACA AGAATTCCAA 
GTCTTTAGAA ACACTGCTCA CCCACTGCAG AGTTCAACAG GATTCACAGA GGGCCTCTCC 
CGACATTTAG TTTTAAACTT AATTTTAAAA CCTGGAGAAA AGAAATCTGT CAAAGTAAAG 
TTTACTCCAG TTCACAATAG AACTGTTTCT TCCCTAATCA TAATCAGAAA TAACCTGACT 
GTGATGGATG CCGTAATGGT CCAAGGACAG GGGACAACCG AGAACCTGAG GGTGGCAGGC 
AAGCTTCCGG GCCCAGGGAG CTCCTTACGC TTCAAAATCA CAGAAGCACT GCTGAAAGAC 
TGTACGGAGG GTATGAGACT AAGGGAACCC AATTTCACAT TGAAAAGAAC ATTTAAAGTA 
GAGAACACAG GACAACTTCC AATTCACATA GAAACCATTG AAGTCAGTGG GTACTCTTGT 
GAGGGATATG GCTTTAAAGT CGTCAATTGT CAAGAGTTTG CACTGAGCGC CAATGCCTCT 
AAAGACATAG TCATTCTGTT TACTCCTGAT TTCACGGCTT CTAGAGTCAT CCGTGAACTG 
AAGTTTATAA CAGCCAGTGG CTCTGAGTTT GTGTTTGTAT TGAATGCATC CCTTCCTTAC 
CATATGTTAG CAACCTGTGC AGAAGCCCTG CCCAGGCCCA ACTGGGAGCT GGCTCTGTAC 
ATCATCATCT CAGGAATAAT GAGTGCGCTG TTTCTCTTGG TAATTGGAAC AGCCTATTTA 
GAAGCTCAAG GAATTTGGGA GCCCTTCCGA AGGCGGCTGT CCTTTGAGGC CTCGAACCCG 
CCCTTTGATG TGGGGAGACC ATTTGATCTC AGGAGAATCG TTGGTATTTC ATCTGAAGGA 
AACTTGAACA CACTCAGCTG TGACCCCGGT CACAGTCGGG GCTTCTGTGG AGCAGGTGGC 
TCGGCCTCCC GGCCCGGCGC AGGGAGTCAC AAGCAGTGTA GCCCGTCGGT CCACCCGCAC 
AGCAGTCACA GCAATAGAAA CTCGGCTGAC GTGGAAAACG TCAGAGCCAA AAACAGTGCG 
AGTGCCTCTG GCAGGACTAA TGCCCAGTCG GCGAGCAAAG CCAGCCCACT GGTCTTCGAG 
TCCCACAGCA TGGCCCAGGG TCACACCGCA GGCCGGAAGT CCAAGGGGGC CAGACAGGGC 
CAGCACAGCA GCCAGCACCA TGGCCACAGC CCCCTGGAAC AGCACTCGCC CCCGCCGCCG 
CCGCCAGTGC CTCAGCAGCA GGAGCCACAG CCCGAGAGGC TGTCCCCTGC GCCCCTCGCA 
CCCCCGCCCC ACCCAGAGCA CCCCGGCAGC ACCCGGCACA GCTCCGAGGA CTCAGACATC 
ACCAGTCTCA TCGAAGCCAT GGACAAAGAC TTCGAGCACC ACGACGCCCC GCCCCTAGAT 
GTGTTTACAG AGCAGCCGCC TTCACCCTTG TCGAAAAGCA AAGGGAAAGG AAAGCCTCCT 
CAGCGCAAGG TGAAGCCCCC CAAGAAGCAG GAGGAGAAGG AGAAGAAGGG AAAGGGGAGG 
GCCCTGGAAG ACGAGCTGAA GGACTCCTTG GCTGACGACG ACAGCTCCTC CACCACCACA 
GAGACCTCCA ACCCAGACAC AGAGCCTCTC CTCAAGGAGG ATACAGAAAA GCAAAAGGGA 
AAACAAGCCA TGCCTGAAAA ACATGAAAAC GAAATGTCTC AAGTGAAACA AAAAAGCAAA 
AAAGTCTTAA ATGTTAAGAA AGAAATCCCA GCAGATGTGA AACCCAGTTG CTTAGAGCTA 
CCGTATACTG CCCCCTTGGA AAGCAAACAG CGCCGAAATC TCCCAACCAA GATTCCTCTT 
CCAACTACAC TGACCAGTGG ATCCAAACCA CGAAACTCCC AGAAAACAAA AGGTACAAGT 
AAGTTAGTGG ATAGCAGGCC ACCTGCCCTC GCAAAGTTTC TCCCAAACAG TCAAGAACTA 
GGCAACACCA GCAGCTCAGA AGGTGAAAAA GACTCTCCAC CACCAGAGTG GGATTCCGTG 
CCTGTTCACA AGCCCGGCAG CTACATCTTC CTGAAACAAC GCCAGACCTC GCCGCCGCCC 
ACCTCCCCCT CACCGCCCGC CGCACCCTGC CCGTTCACGT CCCGGGGCAG CTACAGCAGC 
ATCGTCCATG GCAGCCCGGG CAGCGACTCT AAAGCAAAGC AGCCAAGCGG GAGCAAACAC 
AAACTGACAA AAGCCGCTTC TCTCCCGGGC AAGAATGGCA ACCCCACTTT CGCTGCGGTC 
ACGGCTGGGT ATGACAAGAG CCCAGGTGGG AACGGCTTTG CCAAAGTTTC TTCAAGCAAA 
ACAGATTTCT CTAGCAGCCT TGGCATTTCA CACACTCCTG TTGACAGCGA TGGCTCAGAC 
AGCTCGGGTC TGTGGAGCCC CGTGAGCAAC CCCAGCAGCC CTGACTTCAC ACCCCTCAAC 
TCGTTCTCAG CCTTTGGGAA TTCTTTTAAC CTAACTGGTG AAGTGTTCAG CAAGCTCGGG 
TTATCGCGAT CATGCAATCA GCCCTCACAG AGGAGCTGGA ATGAGCTGAG CAGCGGCCCC 
TCCTACCTCT GGGACTCGCC GGCCACAGAG CCCAGCCCTT CCTGGCCAGC CAGTTCCAGC 
TCCCCGACCC ACACGGCCAC ACCCATCCTT GGCAGCACCA GCAGCCTGTG GTCGTCCACT 
CCGTTCAGCA GCTCCATCTG GTCCAGCAGC CTGCACAGCA CCCTGCCCTT CACCAGCCCC 
ACCAGCGCGT TGCCGAGCAT CAGCCTCATG GGCCCCGAGA GCGCCCCGGC AGCTCACGCG 
CCCTCTGCCG CCAGCCCCTC CGACGACCTG GGCCAGACCT ACAACCCCTG GCGGATATGG 
AGTCCCACCA TCGGACGGAG GAGCTCAGAC CCCTGGTCGA ATTCGCACTT CCCTCACGAG 
AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212460.1
CDS	558..6143
Translation	MGKRAGGGAAGSAAAASTSAVAGLEPAAGRGGGPRSAAAGLLGALHLVMTLVVAAARAEKEAFIQSESIIEVLRFDDGGLLQTETTLGLSSYQQKSISLYRGNCRPIRFEPPMLDFHEQPVGMPKMEKVYLHNPSSEETITLVSISATTSHFHASFFQNRKILPGGNTSFDVVFLARVVGNVENTLFINTSNHGVFTYQVFGVGVPNPYRLRPFLGARVPVNSSFSPIINIHNPHSEPLQVVEMYSSGGDLHLELPTGQQGGTRKLWEIPPYETKGVMRASFSSREADNHTAFIRIKTNASDSTEFIILPVEVEVTTAPGIYSSTEMLDFGTLRTQDLPKVLNLHLLNSGTKDVPITSVRPTPQNDAVTVHFKPITLKASESKYTKVASISFDASKAKKPSQFSGKITVKAKEKSYSKLEIPYQAEVLDGYLGFDHAATLFHIRDSPADPVERPIYLTNTFSFAILIHDVLLPEEAKTMFKVHNFSKPVLILPNGSGYIFTLFFMPSTSSMHIDNNILLITNASKFHLPVRVYTGFLDYFVLPPKIEERFIDFGILSATEASNILFAIINSNPIELAIKSWHIIGDGLSIELVATERGNRTTIISSLPEFEKSSLSDQSSVILASGYFAVFRVKLTAKKLEGIHDGAIQITTDYEILTIPVKAVIAVGSLTCFPKHMVLPPSFPGKIVHQSLNIMNSFSQKVKIQQIRSLSEDVRFYYKRLRGNKEDLEPGKKSKIANIYFDPGLQCGDHCYVGLPFLSKSEPRVQPGIAMQEDAWDTDWDLHQSLFKGWMGIKENSGHKLSAMFEVNTDLQKNILSKVSAELSWPSLLSSPRLVRFPLTNTNCSSEEEIMLENPADVPIYIQFIPLALYSSPSIFVDKLISRFNLSKVAKIDLRTQEFQVFRNTAHPLQSSTGFTEGLSRHLVLNLILKPGEKKSVKVKFTPVHNRTVSSLIIIRNNLTVMDAVMVQGQGTTENLRVAGKLPGPGSSLRFKITEALLKDCTEGMRLREPNFTLKRTFKVENTGQLPIHIETIEVSGYSCEGYGFKVVNCQEFALSANASKDIVILFTPDFTASRVIRELKFITASGSEFVFVLNASLPYHMLATCAEALPRPNWELALYIIISGIMSALFLLVIGTAYLEAQGIWEPFRRRLSFEASNPPFDVGRPFDLRRIVGISSEGNLNTLSCDPGHSRGFCGAGGSASRPGAGSHKQCSPSVHPHSSHSNRNSADVENVRAKNSASASGRTNAQSASKASPLVFESHSMAQGHTAGRKSKGARQGQHSSQHHGHSPLEQHSPPPPPPVPQQQEPQPERLSPAPLAPPPHPEHPGSTRHSSEDSDITSLIEAMDKDFEHHDAPPLDVFTEQPPSPLSKSKGKGKPPQRKVKPPKKQEEKEKKGKGRALEDELKDSLADDDSSSTTTETSNPDTEPLLKEDTEKQKGKQAMPEKHENEMSQVKQKSKKVLNVKKEIPADVKPSCLELPYTAPLESKQRRNLPTKIPLPTTLTSGSKPRNSQKTKGTSKLVDSRPPALAKFLPNSQELGNTSSSEGEKDSPPPEWDSVPVHKPGSYIFLKQRQTSPPPTSPSPPAAPCPFTSRGSYSSIVHGSPGSDSKAKQPSGSKHKLTKAASLPGKNGNPTFAAVTAGYDKSPGGNGFAKVSSSKTDFSSSLGISHTPVDSDGSDSSGLWSPVSNPSSPDFTPLNSFSAFGNSFNLTGEVFSKLGLSRSCNQPSQRSWNELSSGPSYLWDSPATEPSPSWPASSSSPTHTATPILGSTSSLWSSTPFSSSIWSSSLHSTLPFTSPTSALPSISLMGPESAPAAHAPSAASPSDDLGQTYNPWRIWSPTIGRRSSDPWSNSHFPHEN

Target ORF information:

RefSeq Version	XM_005212403.4
Organism	Bos taurus(cattle)
Definition	Bos taurus transmembrane protein 131 (TMEM131), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212403.4

ORF Insert Sequence:

ATGGGGAAGC GGGCCGGAGG AGGAGCAGCG GGATCCGCCG CCGCTGCCTC CACGTCCGCC 
GTGGCCGGTC TGGAGCCCGC GGCCGGCCGC GGCGGGGGCC CGCGGAGCGC AGCCGCCGGC 
CTCCTGGGAG CGCTGCACCT GGTGATGACC CTCGTCGTGG CCGCGGCGCG GGCCGAGAAG 
GAAGCATTTA TTCAGTCGGA GAGCATAATA GAAGTGCTGC GTTTTGATGA TGGAGGGCTT 
CTACAGACTG AGACAACACT TGGACTCAGT TCATACCAAC AGAAAAGTAT ATCTTTGTAC 
CGGGGTAATT GCAGACCTAT ACGGTTTGAG CCACCAATGC TGGATTTCCA TGAACAACCA 
GTTGGAATGC CAAAAATGGA AAAAGTTTAC TTACATAATC CTAGTTCTGA AGAAACTATT 
ACTTTAGTAT CAATATCTGC TACCACATCA CATTTTCACG CATCATTTTT TCAAAATAGG 
AAAATTCTTC CAGGAGGAAA TACATCATTT GATGTAGTTT TTCTTGCAAG AGTAGTGGGA 
AATGTAGAAA ATACTTTATT TATTAATACA TCTAATCATG GGGTATTCAC TTACCAGGTA 
TTTGGTGTTG GAGTTCCAAA TCCATACCGA TTGAGGCCAT TCCTTGGGGC CAGAGTCCCT 
GTGAATAGCA GTTTCTCACC CATAATAAAC ATACACAATC CTCACAGCGA GCCTTTACAG 
GTTGTAGAAA TGTACTCTAG TGGAGGAGAC CTTCACCTTG AACTTCCCAC AGGGCAGCAG 
GGAGGCACCA GGAAGCTGTG GGAAATTCCT CCTTATGAAA CCAAGGGAGT GATGAGAGCC 
AGTTTTTCAT CTAGAGAAGC AGATAATCAC ACAGCTTTCA TAAGAATAAA GACAAATGCT 
TCAGACAGCA CGGAGTTTAT CATCCTTCCG GTTGAAGTGG AAGTCACAAC AGCTCCTGGA 
ATTTATTCCT CAACTGAAAT GTTAGATTTT GGCACACTGC GAACACAAGA TCTACCAAAA 
GTTTTAAATC TTCATTTATT AAATTCAGGA ACAAAAGATG TACCAATAAC AAGTGTTCGA 
CCTACACCAC AAAATGATGC TGTCACTGTA CACTTTAAAC CAATTACATT AAAAGCTTCA 
GAAAGTAAAT ATACCAAGGT AGCAAGTATT AGTTTTGATG CATCAAAGGC GAAAAAACCA 
TCTCAGTTTT CTGGGAAAAT AACTGTGAAA GCAAAGGAAA AGAGTTATTC TAAACTTGAA 
ATACCATATC AAGCAGAGGT TTTAGATGGT TATTTGGGAT TTGATCACGC TGCAACATTA 
TTTCACATTC GAGACAGTCC TGCTGATCCT GTGGAAAGGC CAATTTACCT TACTAACACT 
TTCAGCTTTG CGATCCTCAT TCATGATGTG TTGCTACCAG AAGAAGCCAA AACAATGTTT 
AAAGTTCACA ACTTCAGCAA ACCAGTCTTA ATTCTACCAA ATGGATCAGG CTACATTTTT 
ACTCTATTTT TTATGCCATC CACATCGTCC ATGCACATAG ATAACAATAT TTTACTTATT 
ACCAATGCCT CTAAGTTTCA CTTACCTGTG CGGGTATATA CAGGCTTTTT AGATTACTTT 
GTATTACCCC CCAAAATAGA AGAACGTTTC ATAGATTTTG GTATACTGAG TGCAACAGAA 
GCAAGTAATA TTTTATTTGC CATTATAAAC AGCAATCCAA TTGAGTTGGC TATAAAAAGC 
TGGCATATTA TAGGAGATGG TTTATCAATA GAACTCGTAG CTACTGAAAG AGGCAATAGA 
ACTACAATCA TCTCAAGCCT TCCAGAGTTT GAAAAATCCT CTCTATCAGA CCAGTCATCG 
GTAATACTAG CTTCAGGATA CTTTGCAGTG TTCAGAGTCA AACTTACTGC AAAAAAACTG 
GAAGGAATTC ATGATGGGGC TATACAGATC ACGACAGACT ATGAGATCCT AACAATTCCT 
GTGAAGGCTG TGATCGCAGT GGGTTCTTTG ACTTGCTTCC CAAAGCACAT GGTTCTTCCA 
CCTTCCTTTC CAGGGAAAAT AGTTCATCAG AGTTTAAATA TTATGAATTC CTTCTCACAG 
AAGGTAAAGA TCCAACAGAT ACGATCTTTG TCAGAAGACG TGCGTTTTTA CTACAAACGA 
TTGCGGGGTA ATAAGGAAGA CTTGGAGCCA GGGAAAAAAT CAAAGATTGC AAACATTTAC 
TTTGATCCTG GATTGCAGTG CGGGGATCAT TGCTATGTCG GCTTGCCTTT TCTGTCCAAA 
TCTGAACCCA GAGTCCAGCC TGGCATAGCC ATGCAGGAAG ATGCCTGGGA CACCGACTGG 
GATTTGCATC AAAGCCTATT CAAGGGATGG ATGGGAATAA AGGAAAACTC AGGTCACAAA 
TTGAGCGCTA TGTTTGAAGT GAATACAGAC CTTCAGAAAA ACATCCTGTC AAAAGTTTCT 
GCCGAGCTCT CCTGGCCATC CCTCCTCAGC TCCCCCCGGC TCGTGAGGTT TCCCCTCACT 
AATACAAACT GCTCTTCAGA AGAAGAGATT ATGTTAGAAA ACCCTGCAGA TGTTCCCATC 
TATATTCAAT TTATCCCCCT GGCTTTATAT TCCAGCCCTT CCATCTTTGT AGATAAGTTA 
ATATCAAGGT TTAACTTAAG TAAGGTGGCA AAGATCGATT TAAGAACACA AGAATTCCAA 
GTCTTTAGAA ACACTGCTCA CCCACTGCAG AGTTCAACAG GATTCACAGA GGGCCTCTCC 
CGACATTTAG TTTTAAACTT AATTTTAAAA CCTGGAGAAA AGAAATCTGT CAAAGTAAAG 
TTTACTCCAG TTCACAATAG AACTGTTTCT TCCCTAATCA TAATCAGAAA TAACCTGACT 
GTGATGGATG CCGTAATGGT CCAAGGACAG GGGACAACCG AGAACCTGAG GGTGGCAGGC 
AAGCTTCCGG GCCCAGGGAG CTCCTTACGC TTCAAAATCA CAGAAGCACT GCTGAAAGAC 
TGTACGGAGG GTATGAGACT AAGGGAACCC AATTTCACAT TGAAAAGAAC ATTTAAAGTA 
GAGAACACAG GACAACTTCC AATTCACATA GAAACCATTG AAGTCAGTGG GTACTCTTGT 
GAGGGATATG GCTTTAAAGT CGTCAATTGT CAAGAGTTTG CACTGAGCGC CAATGCCTCT 
AAAGACATAG TCATTCTGTT TACTCCTGAT TTCACGGCTT CTAGAGTCAT CCGTGAACTG 
AAGTTTATAA CAGCCAGTGG CTCTGAGTTT GTGTTTGTAT TGAATGCATC CCTTCCTTAC 
CATATGTTAG CAACCTGTGC AGAAGCCCTG CCCAGGCCCA ACTGGGAGCT GGCTCTGTAC 
ATCATCATCT CAGGAATAAT GAGTGCGCTG TTTCTCTTGG TAATTGGAAC AGCCTATTTA 
GAAGCTCAAG GAATTTGGGA GCCCTTCCGA AGGCGGCTGT CCTTTGAGGC CTCGAACCCG 
CCCTTTGATG TGGGGAGACC ATTTGATCTC AGGAGAATCG TTGGTATTTC ATCTGAAGGA 
AACTTGAACA CACTCAGCTG TGACCCCGGT CACAGTCGGG GCTTCTGTGG AGCAGGTGGC 
TCGGCCTCCC GGCCCGGCGC AGGGAGTCAC AAGCAGTGTA GCCCGTCGGT CCACCCGCAC 
AGCAGTCACA GCAATAGAAA CTCGGCTGAC GTGGAAAACG TCAGAGCCAA AAACAGTGCG 
AGTGCCTCTG GCAGGACTAA TGCCCAGTCG GCGAGCAAAG CCAGCCCACT GGTCTTCGAG 
TCCCACAGCA TGGCCCAGGG TCACACCGCA GGCCGGAAGT CCAAGGGGGC CAGACAGGGC 
CAGCACAGCA GCCAGCACCA TGGCCACAGC CCCCTGGAAC AGCACTCGCC CCCGCCGCCG 
CCGCCAGTGC CTCAGCAGCA GGAGCCACAG CCCGAGAGGC TGTCCCCTGC GCCCCTCGCA 
CCCCCGCCCC ACCCAGAGCA CCCCGGCAGC ACCCGGCACA GCTCCGAGGA CTCAGACATC 
ACCAGTCTCA TCGAAGCCAT GGACAAAGAC TTCGAGCACC ACGACGCCCC GCCCCTAGAT 
GTGTTTACAG AGCAGCCGCC TTCACCCTTG TCGAAAAGCA AAGGGAAAGG AAAGCCTCCT 
CAGCGCAAGG TGAAGCCCCC CAAGAAGCAG GAGGAGAAGG AGAAGAAGGG AAAGGGGAGG 
GCCCTGGAAG ACGAGCTGAA GGACTCCTTG GCTGACGACG ACAGCTCCTC CACCACCACA 
GAGACCTCCA ACCCAGACAC AGAGCCTCTC CTCAAGGAGG ATACAGAAAA GCAAAAGGGA 
AAACAAGCCA TGCCTGAAAA ACATGAAAAC GAAATGTCTC AAGTGAAACA AAAAAGCAAA 
AAAGTCTTAA ATGTTAAGAA AGAAATCCCA GCAGATGTGA AACCCAGTTG CTTAGAGCTA 
CCGTATACTG CCCCCTTGGA AAGCAAACAG CGCCGAAATC TCCCAACCAA GATTCCTCTT 
CCAACTACAC TGACCAGTGG ATCCAAACCA CGAAACTCCC AGAAAACAAA AGGTACAAGT 
AAGTTAGTGG ATAGCAGGCC ACCTGCCCTC GCAAAGTTTC TCCCAAACAG TCAAGAACTA 
GGCAACACCA GCAGCTCAGA AGGTGAAAAA GACTCTCCAC CACCAGAGTG GGATTCCGTG 
CCTGTTCACA AGCCCGGCAG CTACATCTTC CTGAAACAAC GCCAGACCTC GCCGCCGCCC 
ACCTCCCCCT CACCGCCCGC CGCACCCTGC CCGTTCACGT CCCGGGGCAG CTACAGCAGC 
ATCGTCCATG GCAGCCCGGG CAGCGACTCT AAAGCAAAGC AGCCAAGCGG GAGCAAACAC 
AAACTGACAA AAGCCGCTTC TCTCCCGGGC AAGAATGGCA ACCCCACTTT CGCTGCGGTC 
ACGGCTGGGT ATGACAAGAG CCCAGGTGGG AACGGCTTTG CCAAAGTTTC TTCAAGCAAA 
ACAGATTTCT CTAGCAGCCT TGGCATTTCA CACACTCCTG TTGACAGCGA TGGCTCAGAC 
AGCTCGGGTC TGTGGAGCCC CGTGAGCAAC CCCAGCAGCC CTGACTTCAC ACCCCTCAAC 
TCGTTCTCAG CCTTTGGGAA TTCTTTTAAC CTAACTGGTG AAGTGTTCAG CAAGCTCGGG 
TTATCGCGAT CATGCAATCA GCCCTCACAG AGGAGCTGGA ATGAGCTGAG CAGCGGCCCC 
TCCTACCTCT GGGACTCGCC GGCCACAGAG CCCAGCCCTT CCTGGCCAGC CAGTTCCAGC 
TCCCCGACCC ACACGGCCAC ACCCATCCTT GGCAGCACCA GCAGCCTGTG GTCGTCCACT 
CCGTTCAGCA GCTCCATCTG GTCCAGCAGC CTGCACAGCA CCCTGCCCTT CACCAGCCCC 
ACCAGCGCGT TGCCGAGCAT CAGCCTCATG GGCCCCGAGA GCGCCCCGGC AGCTCACGCG 
CCCTCTGCCG CCAGCCCCTC CGACGACCTG GGCCAGACCT ACAACCCCTG GCGGATATGG 
AGTCCCACCA TCGGACGGAG GAGCTCAGAC CCCTGGTCGA ATTCGCACTT CCCTCACGAG 
AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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TMEM131 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

サポート

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