kif20ba cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	kif20ba
Entrez Gene ID	449979
Full Name	kinesin family member 20Ba
Synonyms	im:7142427,im:7142489,im:7144435
General protein information	Preferred Names kinesin family member 20Ba Names kinesin-like protein KIF20B M-phase phosphoprotein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021474376.1	XP_021330051.1	kinesin-like protein KIF20B isoform X1
XM_005158528.3	XP_005158585.1	kinesin-like protein KIF20B isoform X2
XM_003200345.4	XP_003200393.2	kinesin-like protein KIF20B isoform X1
XM_021473145.1	XP_021328820.1	kinesin-like protein KIF20B isoform X2
XM_021474377.1	XP_021330052.1	kinesin-like protein KIF20B isoform X2
XM_003200345.5	XP_003200393.2	kinesin-like protein KIF20B isoform X1
XM_005158528.4	XP_005158585.1	kinesin-like protein KIF20B isoform X2
XM_021473144.1	XP_021328819.1	kinesin-like protein KIF20B isoform X1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006989	XP_308782.4
Danio rerio (zebrafish)	kif20ba	XP_005158585.1
Drosophila melanogaster (fruit fly)	sub	NP_611260.1

Gene Ontology

The following kif20ba gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kif20ba cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa22505	XM_021474376.1 Latest version!	Danio rerio kinesin family member 20Ba (kif20ba), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa22505	XM_021473144.1 Latest version!	Danio rerio kinesin family member 20Ba (kif20ba), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa22505	XM_003200345.5 Latest version!	Danio rerio kinesin family member 20Ba (kif20ba), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa22506	XM_005158528.4 Latest version!	Danio rerio kinesin family member 20Ba (kif20ba), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa22506	XM_021473145.1 Latest version!	Danio rerio kinesin family member 20Ba (kif20ba), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa22506	XM_021474377.1 Latest version!	Danio rerio kinesin family member 20Ba (kif20ba), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa22505	XM_003200345.4	Danio rerio kinesin family member 20Ba (kif20ba), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa22506	XM_005158528.3	Danio rerio kinesin family member 20Ba (kif20ba), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kif20ba ( XM_021474376.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330051.1 Danio rerio kinesin family member 20Ba (kif20ba), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa22505
Clone ID Related Accession (Same CDS sequence)	XM_021474376.1 , XM_003200345.4 , XM_003200345.5 , XM_021473144.1
Accession Version	XM_021474376.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF20B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGT CAGGGCTAAA GGATGATCCA GCAAGACCTG CTATATTAGT TGTAGAAGAT 
CTAAGGAGAA ATTTGTCCAC AGAGTTTGAT TGTTCAGATT TGCAGGACTC GTCAGACTTG 
AAGGAACATC TGAAGGTGTA TCTTAGGATA AGACCATTTT CAGCAGTTGA GATTGAGAAT 
GGAGAATCAC AGGACTGTGT GACCATTCAG CGTCCAGACA CTGTCCTTCT CAAACCTCCA 
CAAACATCTC TGTCTGCCAG GCTCAGTGAT AAATCAGTGC CAGTTACAGC TCAGCGTTTC 
CAGTTCTCTC AGGTCTATGG TCCAGACACT ACACAGAAGG ATATATTTGA TGGCACAGTA 
AACGGACTTG TCAGAAATGT TCTAGAAGGG CAAAATTCTC TGGTCTTTAC GTATGGTGTC 
ACCAATGCTG GGAAGACCTT TACTTTTTTA GGTCCAGACA CTAATCCGGG CATCTTGCCT 
CGTTCCCTCA ACATGATCTT CAGTAGTCTG GAGGGTCGCA TCTTTACCCA GATGTGCCTG 
AAACCCCAGA GGTGCCGAGA TTTCATCAGA CTTACAAAAG AGCAACAAAA TGAGGAAGCA 
GCCAGCAAAC GCAACCTGCT CAAGCTTTTC AAAGAGTCTG ATGCTCAGAG GAGTATTTCT 
GGCCATTGCT CAAAGTCTGA TATTGAAGAT GGTTCCATGC TCTCTGACGT AAACTCTGCG 
GTTTTGGATG TGGAGATGGA CATGCACACT AGGTTTTCTA TTTGGGTCTC GTTCTGTGAG 
ATCTACAATG AGAACATCCA TGACTTGCTA GAACAGCATG CAAGCAATGC TTCACGGAAA 
ACCAACCTTC GCCTTTGTCA GGATGTCAAA GGAAACTCAT TCATTAAAGA TCTAAAATGG 
GTCCAGGTGA GCAGTGCGGA GGAAGCCCTG AAAGTGATGA AACTGGGCAA AAGAAATCAG 
AGCATCTCTA GCACTAAACT CAACCTGCTC TCCAGCAGAA GCCACAGTAT TTTCTCTGTT 
CGAATACTGA AGGTGGAGGA TGTGGGAATA CCTAGAGTGG AGTCAGTCAG CGAGCTGGCA 
CTGTGTGATC TGGCTGGATC AGAGCGTTGT GCTAAGACTC AAAATAAAGG TGAACGGCTA 
AAAGAGGCTG GCAACATCAA TACTTCTCTG CTCAGTCTTG GAAAGTGCAT TAATGCACTA 
AGGAATAACC AACAGGCTAG ACAGCATGTT CCGTTCAGAG AGAGTAAACT CACTCACTAT 
CTGCAGGGCT ACTTCACAGG CCGGGGCTCA GCCTGTATGA TTGTAAACAT TAATCAGTGC 
TCCTCCATGT ATGATGAGAC GCTGAATGTG CTCAAGTTCT CTGCCGTGGC TCAGAAGGTG 
GTGGTCCTCA CCACTAAGAA CATCCCAGTG GGAGTGAAAA GGAGTGCAAG AGAGGTGTCC 
TTCATAATCA ACAACGCTGA TTGCCGGGAA ATGAGACGCA GCTCATTAGT ACGATGGGAC 
CCAAGTCTTG AGGATGTCCA GGAAGATTCT GACTCAGAGG ACGAGTATGA AGACCAGAGC 
GAAATCGAGG ACACCTTACT AAATTCTGAA GATGAATGTA CAGAGACTGT GCAAATCAAT 
AAAAAGCTTT ATGAGGGCCA GCAGCGGTTA ATAGTGAATA CTCAGCAGCA GTTGACAGCA 
GTACAAGCCG AACTCTTAAC CCTAGAAGCA CGTGTTCGAG ATGAAGTGAC AAAAGAATTC 
TCAGAACTGA TTTCCCAGAT GCAGGAAGAT TACAGTGAAC GTATGTCCAG AGAGCGGGAG 
CTGATAGAGG AACGGTGTGA ACGGAGACTG GAGATTCTCA AAAACCTAGT GGAAAAAACG 
GCTATGGAGG ATGACCGTAT GAATTCAACT GTGGCCAAAT CAGAGGAGGA GAAACGTGTC 
TCTCTAGACA GCCTGTTTGA CTCTATGAGT GGTGACCTGG CAGGAATCAG ACATGATGCC 
CAGGCTGCCC AGTCCTGCTT GGTGACCTCT GATCCCAGAG TCTCAGCGAC ATTGCATGAC 
CTTGAGAAAA AGGTCACAGA ACTTTCAGAA CAGCTCAGCA TATCTCAGAA TCAGCTGTTG 
CTCAAAACAA AGGAGCTTGA AACCCAGAGC CGGCTGTCAG ATGAACTGGA GGAGGTGAAG 
AAGACTCTGG AGAGCCACAG GGAAAGAGAA TTTAATCTGA GTGACATGTG CAGACAGAAG 
GATGAGATGA TTGCCAAACT CCAGGCTACT TTGGACCAGC ATGTAGAAAG AAAGGCTGAG 
GATAGAGCTC AAATGGACTC TGTCCAAAAG GAGGTCATCC AGTGCTGTTG CACCATTAAA 
GAGCCCCTCA GCTCCCGGAA TGAAACCAGA AAGCGACGTC AGGAAGGAGA GGGGCTTGAA 
GGACAGCCCC CGCTCAAGAA AGGCCCCCCG GAGGACTTGA AAATGCCTGA AGATGAAGCA 
GATGATGGGC GGGAAGACTC CGCCCTTCAT GAGGTCAAAG GTCAGCTGGA GTGTTGCCTT 
GCTGAAGCCA AACAAAAAGA CAAGCAGATC ACAGACCTTG AGCAGGAATG CCACAGATTA 
CAAGTGTGCA TTCAGGATCA AAAGTCAAAT CTGGATGAAA GAGTGTCACA GGAACATATT 
CTGAAAGAAG ACGTTTGTCA GACAACATTG GAAAGCAAAC AGATGGCAAC GGCTGTGGAT 
TTTAGCAATC TGAACGTAGC AGTTGCATCC AGATGTAGCA ACACTACAGG TGCGTTTGCT 
GCTAAATCAC TAATGGCTAA GCGAGATCTT GAACTGAGAG AAAAGAACAA ACACATCTTG 
ACTCTTGAAA AGGAAATTGC GCTAATGAGA CAGAAAGCAG AACACACATC TGACTATGAA 
AATCTCAAAA AACTCAACTC TGAACTGCAA GCTGAGGTCA CGAGCTTATG TAAGGTCAAA 
GCCGAACTTG AGGAGAAGAT TACATGCCTT CAGAAGGAGA AGGAGGGGCT TGAAGATAAG 
GAGGTGGGGC TGACAAACCC TAACACTCTT GGATTTAAAA GTAAAGAGAG AGATAACCAA 
ACACACCTCA TGCTGTCTGG AAAGGAGGCT GAACTTGCAC TGAGAGAGAA TGCTCTAACT 
TTAAAGGAAA CTCAGCTTTC AGCACTACAG AAGAGTCTGA AGCAAACACA GGAGCGTCTG 
GAGGAGGAGG AGATGCAGGC GGTGCAGGAG GCGCGCAGGA AGGAGGTGGA GAGGAGGAGA 
GAGATCCTGG CTGTGGCAGA GGAGGCCATC GCTCAGAAAG ATGCAGAGCT ACAGAAGAGA 
CAAGTAGAGA TCAGCCGGTT AAAAGAGGAG ATTAAAACTA GCGAGTTTAA GATGAGCAGT 
CTGACTGTTG ATCTGCGCAG AAGGGAAGAT GATTCATCTG ATCTTCGGGA AAAACTTTGT 
GACTCAAAGA AACAAATACA GCAGGTCCAG AAAGAGATTT CGTCCATGCG TGAAGCTGAG 
AAATCTCTAC GACTGAAGCT CAGCGATCTA GAAAAAACGA AAGTGCAGCT CCAGAATGAG 
ATCGCCAACA GAGATCGCAC CATTAACCAG TTAAAAGCTG AACGATCCTC TGGTTCAAAG 
TCAGATGAGA ATCTGCTGCT CTATCAGAAA GCATGTAAAG ATCTGCAAGA GCGTGAGCGT 
GTTATTGAAG ACATGCGCTT GGCTCTGACA GAACAGGAGG AAACACAGAC ACAGCTGGAC 
CTTGAGTTGG ACAACAGAGA CGCTCGGATT GATGAGCTCA CTCAAGAGCT GGCCAGTTTG 
AGAGAAATGT TGCCTGAAGA GAGGGACAGG AGTGATTCCA GACCAATCTC TAAAGATGTT 
TTACAGGCCA GACAGCAGAT CACTCAGGCC CACGAGAGCT TACAGCTTGC CAATGAGAAG 
CAGCAGGCAG AACGCAAGAA GTGGATGGAG GAGAAGTTGA TTCTGATTGG TCAGGCTAAA 
GAAGCTGAGG AGAGGCGGAA TCAGGACATG AGACGATTTG CAGATGATCG AGAACGGCAT 
GTTCGGCAAC AGGCTGAAAT GGAGTCTCTG GCTGCACGTC TTAATGCCCG TGAGGAAGAG 
ATGGAAAGGT GGAGAAAGGA GAGAGACACG CTTGTCTCTG CTCTGGAGGT TCAGCTGAAG 
AAACTCATCA TGTCCAATGG AGAAAAAGAC CAACAGATCA AGATGCTGCA GTCCAGTAAC 
ACTGCCCAAA CACCAGAGGA GGTCAGTGAG GACAGCAGGA CCGAGGCACT GCAGGCAGAG 
CTGGCTGTAA AAGAGGCTGA GATCCAGAAA CTGAAGGAGC AGCTCAGCAT CAGTGCCACA 
AACAGCAGCA TTCGCAGAAA CTCTACTACA CAGACGATGG AAGTGGAGTT TTCTGATATG 
AAGCCTCTGA ACAGCAGTAC AATCAAGCAC AAAAGCCGAA CCAGAGGATC TGTCATCAGT 
CAGAACTCAT CCGGTAGCGG TCCACTGGTG CTAGATTCAT CAGAGATCTC CACGGAAACA 
GGCAGACGGT CTAGGTTTCC AAGGCCTGAG CTGGAGATTT CCTTCAGTCC TCTACAGCCC 
GATCGCTTCG CTCTCAAGCG TCAGGGTGAA GAATCTGCTG TCACTGTTAA AATCTCCAGA 
CCCACACGCA AGAGGAAGAG CGGCGAGATG GACAAGAGGA AAGGAGCCAG GAGCAGCGCT 
GTGAAAAAGA AAGCTTTCTC TGAGCCCAAT CCACAGGATG CGGTGGAAGG TGAAAACATC 
AGAAATACAA GGTGCAGATT GACTCCTCAC CTAACACCTC ATCGGGAAGA GTCCCCAAAT 
TCCCAGGAGT CAAACCAAAG AGGGAAAAAA GACAAAACGC TCCAGAAGAT TGGAGATTTT 
CTTCAGAGCT CTCCCACCTT TCTTGGGAGT AAAGCGAAGA GGATCATGGG ATTGATGAGT 
GGAAAATCCC CTGATGGTGG GTCGACTCCT TCTCTAAAAC TGAAATCCAA ACGTGATAAA 
CGGAAATATG ACAGACCCGA TATCTCCTCA CCAATGGACA TACCGGCTCA TCGGATTATT 
GGCCGAAATC CAGAGGACAA AGAGAGTGAA AACCTTATCA AGAAACGTCT TCGTACAAGA 
ATGGCCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330051.1
CDS	149..5260
Translation	MMESGLKDDPARPAILVVEDLRRNLSTEFDCSDLQDSSDLKEHLKVYLRIRPFSAVEIENGESQDCVTIQRPDTVLLKPPQTSLSARLSDKSVPVTAQRFQFSQVYGPDTTQKDIFDGTVNGLVRNVLEGQNSLVFTYGVTNAGKTFTFLGPDTNPGILPRSLNMIFSSLEGRIFTQMCLKPQRCRDFIRLTKEQQNEEAASKRNLLKLFKESDAQRSISGHCSKSDIEDGSMLSDVNSAVLDVEMDMHTRFSIWVSFCEIYNENIHDLLEQHASNASRKTNLRLCQDVKGNSFIKDLKWVQVSSAEEALKVMKLGKRNQSISSTKLNLLSSRSHSIFSVRILKVEDVGIPRVESVSELALCDLAGSERCAKTQNKGERLKEAGNINTSLLSLGKCINALRNNQQARQHVPFRESKLTHYLQGYFTGRGSACMIVNINQCSSMYDETLNVLKFSAVAQKVVVLTTKNIPVGVKRSAREVSFIINNADCREMRRSSLVRWDPSLEDVQEDSDSEDEYEDQSEIEDTLLNSEDECTETVQINKKLYEGQQRLIVNTQQQLTAVQAELLTLEARVRDEVTKEFSELISQMQEDYSERMSRERELIEERCERRLEILKNLVEKTAMEDDRMNSTVAKSEEEKRVSLDSLFDSMSGDLAGIRHDAQAAQSCLVTSDPRVSATLHDLEKKVTELSEQLSISQNQLLLKTKELETQSRLSDELEEVKKTLESHREREFNLSDMCRQKDEMIAKLQATLDQHVERKAEDRAQMDSVQKEVIQCCCTIKEPLSSRNETRKRRQEGEGLEGQPPLKKGPPEDLKMPEDEADDGREDSALHEVKGQLECCLAEAKQKDKQITDLEQECHRLQVCIQDQKSNLDERVSQEHILKEDVCQTTLESKQMATAVDFSNLNVAVASRCSNTTGAFAAKSLMAKRDLELREKNKHILTLEKEIALMRQKAEHTSDYENLKKLNSELQAEVTSLCKVKAELEEKITCLQKEKEGLEDKEVGLTNPNTLGFKSKERDNQTHLMLSGKEAELALRENALTLKETQLSALQKSLKQTQERLEEEEMQAVQEARRKEVERRREILAVAEEAIAQKDAELQKRQVEISRLKEEIKTSEFKMSSLTVDLRRREDDSSDLREKLCDSKKQIQQVQKEISSMREAEKSLRLKLSDLEKTKVQLQNEIANRDRTINQLKAERSSGSKSDENLLLYQKACKDLQERERVIEDMRLALTEQEETQTQLDLELDNRDARIDELTQELASLREMLPEERDRSDSRPISKDVLQARQQITQAHESLQLANEKQQAERKKWMEEKLILIGQAKEAEERRNQDMRRFADDRERHVRQQAEMESLAARLNAREEEMERWRKERDTLVSALEVQLKKLIMSNGEKDQQIKMLQSSNTAQTPEEVSEDSRTEALQAELAVKEAEIQKLKEQLSISATNSSIRRNSTTQTMEVEFSDMKPLNSSTIKHKSRTRGSVISQNSSGSGPLVLDSSEISTETGRRSRFPRPELEISFSPLQPDRFALKRQGEESAVTVKISRPTRKRKSGEMDKRKGARSSAVKKKAFSEPNPQDAVEGENIRNTRCRLTPHLTPHREESPNSQESNQRGKKDKTLQKIGDFLQSSPTFLGSKAKRIMGLMSGKSPDGGSTPSLKLKSKRDKRKYDRPDISSPMDIPAHRIIGRNPEDKESENLIKKRLRTRMAK

Target ORF information:

RefSeq Version	XM_021474376.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 20Ba (kif20ba), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474376.1

ORF Insert Sequence:

ATGATGGAGT CAGGGCTAAA GGATGATCCA GCAAGACCTG CTATATTAGT TGTAGAAGAT 
CTAAGGAGAA ATTTGTCCAC AGAGTTTGAT TGTTCAGATT TGCAGGACTC GTCAGACTTG 
AAGGAACATC TGAAGGTGTA TCTTAGGATA AGACCATTTT CAGCAGTTGA GATTGAGAAT 
GGAGAATCAC AGGACTGTGT GACCATTCAG CGTCCAGACA CTGTCCTTCT CAAACCTCCA 
CAAACATCTC TGTCTGCCAG GCTCAGTGAT AAATCAGTGC CAGTTACAGC TCAGCGTTTC 
CAGTTCTCTC AGGTCTATGG TCCAGACACT ACACAGAAGG ATATATTTGA TGGCACAGTA 
AACGGACTTG TCAGAAATGT TCTAGAAGGG CAAAATTCTC TGGTCTTTAC GTATGGTGTC 
ACCAATGCTG GGAAGACCTT TACTTTTTTA GGTCCAGACA CTAATCCGGG CATCTTGCCT 
CGTTCCCTCA ACATGATCTT CAGTAGTCTG GAGGGTCGCA TCTTTACCCA GATGTGCCTG 
AAACCCCAGA GGTGCCGAGA TTTCATCAGA CTTACAAAAG AGCAACAAAA TGAGGAAGCA 
GCCAGCAAAC GCAACCTGCT CAAGCTTTTC AAAGAGTCTG ATGCTCAGAG GAGTATTTCT 
GGCCATTGCT CAAAGTCTGA TATTGAAGAT GGTTCCATGC TCTCTGACGT AAACTCTGCG 
GTTTTGGATG TGGAGATGGA CATGCACACT AGGTTTTCTA TTTGGGTCTC GTTCTGTGAG 
ATCTACAATG AGAACATCCA TGACTTGCTA GAACAGCATG CAAGCAATGC TTCACGGAAA 
ACCAACCTTC GCCTTTGTCA GGATGTCAAA GGAAACTCAT TCATTAAAGA TCTAAAATGG 
GTCCAGGTGA GCAGTGCGGA GGAAGCCCTG AAAGTGATGA AACTGGGCAA AAGAAATCAG 
AGCATCTCTA GCACTAAACT CAACCTGCTC TCCAGCAGAA GCCACAGTAT TTTCTCTGTT 
CGAATACTGA AGGTGGAGGA TGTGGGAATA CCTAGAGTGG AGTCAGTCAG CGAGCTGGCA 
CTGTGTGATC TGGCTGGATC AGAGCGTTGT GCTAAGACTC AAAATAAAGG TGAACGGCTA 
AAAGAGGCTG GCAACATCAA TACTTCTCTG CTCAGTCTTG GAAAGTGCAT TAATGCACTA 
AGGAATAACC AACAGGCTAG ACAGCATGTT CCGTTCAGAG AGAGTAAACT CACTCACTAT 
CTGCAGGGCT ACTTCACAGG CCGGGGCTCA GCCTGTATGA TTGTAAACAT TAATCAGTGC 
TCCTCCATGT ATGATGAGAC GCTGAATGTG CTCAAGTTCT CTGCCGTGGC TCAGAAGGTG 
GTGGTCCTCA CCACTAAGAA CATCCCAGTG GGAGTGAAAA GGAGTGCAAG AGAGGTGTCC 
TTCATAATCA ACAACGCTGA TTGCCGGGAA ATGAGACGCA GCTCATTAGT ACGATGGGAC 
CCAAGTCTTG AGGATGTCCA GGAAGATTCT GACTCAGAGG ACGAGTATGA AGACCAGAGC 
GAAATCGAGG ACACCTTACT AAATTCTGAA GATGAATGTA CAGAGACTGT GCAAATCAAT 
AAAAAGCTTT ATGAGGGCCA GCAGCGGTTA ATAGTGAATA CTCAGCAGCA GTTGACAGCA 
GTACAAGCCG AACTCTTAAC CCTAGAAGCA CGTGTTCGAG ATGAAGTGAC AAAAGAATTC 
TCAGAACTGA TTTCCCAGAT GCAGGAAGAT TACAGTGAAC GTATGTCCAG AGAGCGGGAG 
CTGATAGAGG AACGGTGTGA ACGGAGACTG GAGATTCTCA AAAACCTAGT GGAAAAAACG 
GCTATGGAGG ATGACCGTAT GAATTCAACT GTGGCCAAAT CAGAGGAGGA GAAACGTGTC 
TCTCTAGACA GCCTGTTTGA CTCTATGAGT GGTGACCTGG CAGGAATCAG ACATGATGCC 
CAGGCTGCCC AGTCCTGCTT GGTGACCTCT GATCCCAGAG TCTCAGCGAC ATTGCATGAC 
CTTGAGAAAA AGGTCACAGA ACTTTCAGAA CAGCTCAGCA TATCTCAGAA TCAGCTGTTG 
CTCAAAACAA AGGAGCTTGA AACCCAGAGC CGGCTGTCAG ATGAACTGGA GGAGGTGAAG 
AAGACTCTGG AGAGCCACAG GGAAAGAGAA TTTAATCTGA GTGACATGTG CAGACAGAAG 
GATGAGATGA TTGCCAAACT CCAGGCTACT TTGGACCAGC ATGTAGAAAG AAAGGCTGAG 
GATAGAGCTC AAATGGACTC TGTCCAAAAG GAGGTCATCC AGTGCTGTTG CACCATTAAA 
GAGCCCCTCA GCTCCCGGAA TGAAACCAGA AAGCGACGTC AGGAAGGAGA GGGGCTTGAA 
GGACAGCCCC CGCTCAAGAA AGGCCCCCCG GAGGACTTGA AAATGCCTGA AGATGAAGCA 
GATGATGGGC GGGAAGACTC CGCCCTTCAT GAGGTCAAAG GTCAGCTGGA GTGTTGCCTT 
GCTGAAGCCA AACAAAAAGA CAAGCAGATC ACAGACCTTG AGCAGGAATG CCACAGATTA 
CAAGTGTGCA TTCAGGATCA AAAGTCAAAT CTGGATGAAA GAGTGTCACA GGAACATATT 
CTGAAAGAAG ACGTTTGTCA GACAACATTG GAAAGCAAAC AGATGGCAAC GGCTGTGGAT 
TTTAGCAATC TGAACGTAGC AGTTGCATCC AGATGTAGCA ACACTACAGG TGCGTTTGCT 
GCTAAATCAC TAATGGCTAA GCGAGATCTT GAACTGAGAG AAAAGAACAA ACACATCTTG 
ACTCTTGAAA AGGAAATTGC GCTAATGAGA CAGAAAGCAG AACACACATC TGACTATGAA 
AATCTCAAAA AACTCAACTC TGAACTGCAA GCTGAGGTCA CGAGCTTATG TAAGGTCAAA 
GCCGAACTTG AGGAGAAGAT TACATGCCTT CAGAAGGAGA AGGAGGGGCT TGAAGATAAG 
GAGGTGGGGC TGACAAACCC TAACACTCTT GGATTTAAAA GTAAAGAGAG AGATAACCAA 
ACACACCTCA TGCTGTCTGG AAAGGAGGCT GAACTTGCAC TGAGAGAGAA TGCTCTAACT 
TTAAAGGAAA CTCAGCTTTC AGCACTACAG AAGAGTCTGA AGCAAACACA GGAGCGTCTG 
GAGGAGGAGG AGATGCAGGC GGTGCAGGAG GCGCGCAGGA AGGAGGTGGA GAGGAGGAGA 
GAGATCCTGG CTGTGGCAGA GGAGGCCATC GCTCAGAAAG ATGCAGAGCT ACAGAAGAGA 
CAAGTAGAGA TCAGCCGGTT AAAAGAGGAG ATTAAAACTA GCGAGTTTAA GATGAGCAGT 
CTGACTGTTG ATCTGCGCAG AAGGGAAGAT GATTCATCTG ATCTTCGGGA AAAACTTTGT 
GACTCAAAGA AACAAATACA GCAGGTCCAG AAAGAGATTT CGTCCATGCG TGAAGCTGAG 
AAATCTCTAC GACTGAAGCT CAGCGATCTA GAAAAAACGA AAGTGCAGCT CCAGAATGAG 
ATCGCCAACA GAGATCGCAC CATTAACCAG TTAAAAGCTG AACGATCCTC TGGTTCAAAG 
TCAGATGAGA ATCTGCTGCT CTATCAGAAA GCATGTAAAG ATCTGCAAGA GCGTGAGCGT 
GTTATTGAAG ACATGCGCTT GGCTCTGACA GAACAGGAGG AAACACAGAC ACAGCTGGAC 
CTTGAGTTGG ACAACAGAGA CGCTCGGATT GATGAGCTCA CTCAAGAGCT GGCCAGTTTG 
AGAGAAATGT TGCCTGAAGA GAGGGACAGG AGTGATTCCA GACCAATCTC TAAAGATGTT 
TTACAGGCCA GACAGCAGAT CACTCAGGCC CACGAGAGCT TACAGCTTGC CAATGAGAAG 
CAGCAGGCAG AACGCAAGAA GTGGATGGAG GAGAAGTTGA TTCTGATTGG TCAGGCTAAA 
GAAGCTGAGG AGAGGCGGAA TCAGGACATG AGACGATTTG CAGATGATCG AGAACGGCAT 
GTTCGGCAAC AGGCTGAAAT GGAGTCTCTG GCTGCACGTC TTAATGCCCG TGAGGAAGAG 
ATGGAAAGGT GGAGAAAGGA GAGAGACACG CTTGTCTCTG CTCTGGAGGT TCAGCTGAAG 
AAACTCATCA TGTCCAATGG AGAAAAAGAC CAACAGATCA AGATGCTGCA GTCCAGTAAC 
ACTGCCCAAA CACCAGAGGA GGTCAGTGAG GACAGCAGGA CCGAGGCACT GCAGGCAGAG 
CTGGCTGTAA AAGAGGCTGA GATCCAGAAA CTGAAGGAGC AGCTCAGCAT CAGTGCCACA 
AACAGCAGCA TTCGCAGAAA CTCTACTACA CAGACGATGG AAGTGGAGTT TTCTGATATG 
AAGCCTCTGA ACAGCAGTAC AATCAAGCAC AAAAGCCGAA CCAGAGGATC TGTCATCAGT 
CAGAACTCAT CCGGTAGCGG TCCACTGGTG CTAGATTCAT CAGAGATCTC CACGGAAACA 
GGCAGACGGT CTAGGTTTCC AAGGCCTGAG CTGGAGATTT CCTTCAGTCC TCTACAGCCC 
GATCGCTTCG CTCTCAAGCG TCAGGGTGAA GAATCTGCTG TCACTGTTAA AATCTCCAGA 
CCCACACGCA AGAGGAAGAG CGGCGAGATG GACAAGAGGA AAGGAGCCAG GAGCAGCGCT 
GTGAAAAAGA AAGCTTTCTC TGAGCCCAAT CCACAGGATG CGGTGGAAGG TGAAAACATC 
AGAAATACAA GGTGCAGATT GACTCCTCAC CTAACACCTC ATCGGGAAGA GTCCCCAAAT 
TCCCAGGAGT CAAACCAAAG AGGGAAAAAA GACAAAACGC TCCAGAAGAT TGGAGATTTT 
CTTCAGAGCT CTCCCACCTT TCTTGGGAGT AAAGCGAAGA GGATCATGGG ATTGATGAGT 
GGAAAATCCC CTGATGGTGG GTCGACTCCT TCTCTAAAAC TGAAATCCAA ACGTGATAAA 
CGGAAATATG ACAGACCCGA TATCTCCTCA CCAATGGACA TACCGGCTCA TCGGATTATT 
GGCCGAAATC CAGAGGACAA AGAGAGTGAA AACCTTATCA AGAAACGTCT TCGTACAAGA 
ATGGCCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif20ba ( XM_003200345.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003200393.2 Danio rerio kinesin family member 20Ba (kif20ba), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa22505
Clone ID Related Accession (Same CDS sequence)	XM_021474376.1 , XM_003200345.4 , XM_003200345.5 , XM_021473144.1
Accession Version	XM_003200345.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF20B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003200345.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGT CAGGGCTAAA GGATGATCCA GCAAGACCTG CTATATTAGT TGTAGAAGAT 
CTAAGGAGAA ATTTGTCCAC AGAGTTTGAT TGTTCAGATT TGCAGGACTC GTCAGACTTG 
AAGGAACATC TGAAGGTGTA TCTTAGGATA AGACCATTTT CAGCAGTTGA GATTGAGAAT 
GGAGAATCAC AGGACTGTGT GACCATTCAG CGTCCAGACA CTGTCCTTCT CAAACCTCCA 
CAAACATCTC TGTCTGCCAG GCTCAGTGAT AAATCAGTGC CAGTTACAGC TCAGCGTTTC 
CAGTTCTCTC AGGTCTATGG TCCAGACACT ACACAGAAGG ATATATTTGA TGGCACAGTA 
AACGGACTTG TCAGAAATGT TCTAGAAGGG CAAAATTCTC TGGTCTTTAC GTATGGTGTC 
ACCAATGCTG GGAAGACCTT TACTTTTTTA GGTCCAGACA CTAATCCGGG CATCTTGCCT 
CGTTCCCTCA ACATGATCTT CAGTAGTCTG GAGGGTCGCA TCTTTACCCA GATGTGCCTG 
AAACCCCAGA GGTGCCGAGA TTTCATCAGA CTTACAAAAG AGCAACAAAA TGAGGAAGCA 
GCCAGCAAAC GCAACCTGCT CAAGCTTTTC AAAGAGTCTG ATGCTCAGAG GAGTATTTCT 
GGCCATTGCT CAAAGTCTGA TATTGAAGAT GGTTCCATGC TCTCTGACGT AAACTCTGCG 
GTTTTGGATG TGGAGATGGA CATGCACACT AGGTTTTCTA TTTGGGTCTC GTTCTGTGAG 
ATCTACAATG AGAACATCCA TGACTTGCTA GAACAGCATG CAAGCAATGC TTCACGGAAA 
ACCAACCTTC GCCTTTGTCA GGATGTCAAA GGAAACTCAT TCATTAAAGA TCTAAAATGG 
GTCCAGGTGA GCAGTGCGGA GGAAGCCCTG AAAGTGATGA AACTGGGCAA AAGAAATCAG 
AGCATCTCTA GCACTAAACT CAACCTGCTC TCCAGCAGAA GCCACAGTAT TTTCTCTGTT 
CGAATACTGA AGGTGGAGGA TGTGGGAATA CCTAGAGTGG AGTCAGTCAG CGAGCTGGCA 
CTGTGTGATC TGGCTGGATC AGAGCGTTGT GCTAAGACTC AAAATAAAGG TGAACGGCTA 
AAAGAGGCTG GCAACATCAA TACTTCTCTG CTCAGTCTTG GAAAGTGCAT TAATGCACTA 
AGGAATAACC AACAGGCTAG ACAGCATGTT CCGTTCAGAG AGAGTAAACT CACTCACTAT 
CTGCAGGGCT ACTTCACAGG CCGGGGCTCA GCCTGTATGA TTGTAAACAT TAATCAGTGC 
TCCTCCATGT ATGATGAGAC GCTGAATGTG CTCAAGTTCT CTGCCGTGGC TCAGAAGGTG 
GTGGTCCTCA CCACTAAGAA CATCCCAGTG GGAGTGAAAA GGAGTGCAAG AGAGGTGTCC 
TTCATAATCA ACAACGCTGA TTGCCGGGAA ATGAGACGCA GCTCATTAGT ACGATGGGAC 
CCAAGTCTTG AGGATGTCCA GGAAGATTCT GACTCAGAGG ACGAGTATGA AGACCAGAGC 
GAAATCGAGG ACACCTTACT AAATTCTGAA GATGAATGTA CAGAGACTGT GCAAATCAAT 
AAAAAGCTTT ATGAGGGCCA GCAGCGGTTA ATAGTGAATA CTCAGCAGCA GTTGACAGCA 
GTACAAGCCG AACTCTTAAC CCTAGAAGCA CGTGTTCGAG ATGAAGTGAC AAAAGAATTC 
TCAGAACTGA TTTCCCAGAT GCAGGAAGAT TACAGTGAAC GTATGTCCAG AGAGCGGGAG 
CTGATAGAGG AACGGTGTGA ACGGAGACTG GAGATTCTCA AAAACCTAGT GGAAAAAACG 
GCTATGGAGG ATGACCGTAT GAATTCAACT GTGGCCAAAT CAGAGGAGGA GAAACGTGTC 
TCTCTAGACA GCCTGTTTGA CTCTATGAGT GGTGACCTGG CAGGAATCAG ACATGATGCC 
CAGGCTGCCC AGTCCTGCTT GGTGACCTCT GATCCCAGAG TCTCAGCGAC ATTGCATGAC 
CTTGAGAAAA AGGTCACAGA ACTTTCAGAA CAGCTCAGCA TATCTCAGAA TCAGCTGTTG 
CTCAAAACAA AGGAGCTTGA AACCCAGAGC CGGCTGTCAG ATGAACTGGA GGAGGTGAAG 
AAGACTCTGG AGAGCCACAG GGAAAGAGAA TTTAATCTGA GTGACATGTG CAGACAGAAG 
GATGAGATGA TTGCCAAACT CCAGGCTACT TTGGACCAGC ATGTAGAAAG AAAGGCTGAG 
GATAGAGCTC AAATGGACTC TGTCCAAAAG GAGGTCATCC AGTGCTGTTG CACCATTAAA 
GAGCCCCTCA GCTCCCGGAA TGAAACCAGA AAGCGACGTC AGGAAGGAGA GGGGCTTGAA 
GGACAGCCCC CGCTCAAGAA AGGCCCCCCG GAGGACTTGA AAATGCCTGA AGATGAAGCA 
GATGATGGGC GGGAAGACTC CGCCCTTCAT GAGGTCAAAG GTCAGCTGGA GTGTTGCCTT 
GCTGAAGCCA AACAAAAAGA CAAGCAGATC ACAGACCTTG AGCAGGAATG CCACAGATTA 
CAAGTGTGCA TTCAGGATCA AAAGTCAAAT CTGGATGAAA GAGTGTCACA GGAACATATT 
CTGAAAGAAG ACGTTTGTCA GACAACATTG GAAAGCAAAC AGATGGCAAC GGCTGTGGAT 
TTTAGCAATC TGAACGTAGC AGTTGCATCC AGATGTAGCA ACACTACAGG TGCGTTTGCT 
GCTAAATCAC TAATGGCTAA GCGAGATCTT GAACTGAGAG AAAAGAACAA ACACATCTTG 
ACTCTTGAAA AGGAAATTGC GCTAATGAGA CAGAAAGCAG AACACACATC TGACTATGAA 
AATCTCAAAA AACTCAACTC TGAACTGCAA GCTGAGGTCA CGAGCTTATG TAAGGTCAAA 
GCCGAACTTG AGGAGAAGAT TACATGCCTT CAGAAGGAGA AGGAGGGGCT TGAAGATAAG 
GAGGTGGGGC TGACAAACCC TAACACTCTT GGATTTAAAA GTAAAGAGAG AGATAACCAA 
ACACACCTCA TGCTGTCTGG AAAGGAGGCT GAACTTGCAC TGAGAGAGAA TGCTCTAACT 
TTAAAGGAAA CTCAGCTTTC AGCACTACAG AAGAGTCTGA AGCAAACACA GGAGCGTCTG 
GAGGAGGAGG AGATGCAGGC GGTGCAGGAG GCGCGCAGGA AGGAGGTGGA GAGGAGGAGA 
GAGATCCTGG CTGTGGCAGA GGAGGCCATC GCTCAGAAAG ATGCAGAGCT ACAGAAGAGA 
CAAGTAGAGA TCAGCCGGTT AAAAGAGGAG ATTAAAACTA GCGAGTTTAA GATGAGCAGT 
CTGACTGTTG ATCTGCGCAG AAGGGAAGAT GATTCATCTG ATCTTCGGGA AAAACTTTGT 
GACTCAAAGA AACAAATACA GCAGGTCCAG AAAGAGATTT CGTCCATGCG TGAAGCTGAG 
AAATCTCTAC GACTGAAGCT CAGCGATCTA GAAAAAACGA AAGTGCAGCT CCAGAATGAG 
ATCGCCAACA GAGATCGCAC CATTAACCAG TTAAAAGCTG AACGATCCTC TGGTTCAAAG 
TCAGATGAGA ATCTGCTGCT CTATCAGAAA GCATGTAAAG ATCTGCAAGA GCGTGAGCGT 
GTTATTGAAG ACATGCGCTT GGCTCTGACA GAACAGGAGG AAACACAGAC ACAGCTGGAC 
CTTGAGTTGG ACAACAGAGA CGCTCGGATT GATGAGCTCA CTCAAGAGCT GGCCAGTTTG 
AGAGAAATGT TGCCTGAAGA GAGGGACAGG AGTGATTCCA GACCAATCTC TAAAGATGTT 
TTACAGGCCA GACAGCAGAT CACTCAGGCC CACGAGAGCT TACAGCTTGC CAATGAGAAG 
CAGCAGGCAG AACGCAAGAA GTGGATGGAG GAGAAGTTGA TTCTGATTGG TCAGGCTAAA 
GAAGCTGAGG AGAGGCGGAA TCAGGACATG AGACGATTTG CAGATGATCG AGAACGGCAT 
GTTCGGCAAC AGGCTGAAAT GGAGTCTCTG GCTGCACGTC TTAATGCCCG TGAGGAAGAG 
ATGGAAAGGT GGAGAAAGGA GAGAGACACG CTTGTCTCTG CTCTGGAGGT TCAGCTGAAG 
AAACTCATCA TGTCCAATGG AGAAAAAGAC CAACAGATCA AGATGCTGCA GTCCAGTAAC 
ACTGCCCAAA CACCAGAGGA GGTCAGTGAG GACAGCAGGA CCGAGGCACT GCAGGCAGAG 
CTGGCTGTAA AAGAGGCTGA GATCCAGAAA CTGAAGGAGC AGCTCAGCAT CAGTGCCACA 
AACAGCAGCA TTCGCAGAAA CTCTACTACA CAGACGATGG AAGTGGAGTT TTCTGATATG 
AAGCCTCTGA ACAGCAGTAC AATCAAGCAC AAAAGCCGAA CCAGAGGATC TGTCATCAGT 
CAGAACTCAT CCGGTAGCGG TCCACTGGTG CTAGATTCAT CAGAGATCTC CACGGAAACA 
GGCAGACGGT CTAGGTTTCC AAGGCCTGAG CTGGAGATTT CCTTCAGTCC TCTACAGCCC 
GATCGCTTCG CTCTCAAGCG TCAGGGTGAA GAATCTGCTG TCACTGTTAA AATCTCCAGA 
CCCACACGCA AGAGGAAGAG CGGCGAGATG GACAAGAGGA AAGGAGCCAG GAGCAGCGCT 
GTGAAAAAGA AAGCTTTCTC TGAGCCCAAT CCACAGGATG CGGTGGAAGG TGAAAACATC 
AGAAATACAA GGTGCAGATT GACTCCTCAC CTAACACCTC ATCGGGAAGA GTCCCCAAAT 
TCCCAGGAGT CAAACCAAAG AGGGAAAAAA GACAAAACGC TCCAGAAGAT TGGAGATTTT 
CTTCAGAGCT CTCCCACCTT TCTTGGGAGT AAAGCGAAGA GGATCATGGG ATTGATGAGT 
GGAAAATCCC CTGATGGTGG GTCGACTCCT TCTCTAAAAC TGAAATCCAA ACGTGATAAA 
CGGAAATATG ACAGACCCGA TATCTCCTCA CCAATGGACA TACCGGCTCA TCGGATTATT 
GGCCGAAATC CAGAGGACAA AGAGAGTGAA AACCTTATCA AGAAACGTCT TCGTACAAGA 
ATGGCCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003200393.2
CDS	154..5265
Translation	MMESGLKDDPARPAILVVEDLRRNLSTEFDCSDLQDSSDLKEHLKVYLRIRPFSAVEIENGESQDCVTIQRPDTVLLKPPQTSLSARLSDKSVPVTAQRFQFSQVYGPDTTQKDIFDGTVNGLVRNVLEGQNSLVFTYGVTNAGKTFTFLGPDTNPGILPRSLNMIFSSLEGRIFTQMCLKPQRCRDFIRLTKEQQNEEAASKRNLLKLFKESDAQRSISGHCSKSDIEDGSMLSDVNSAVLDVEMDMHTRFSIWVSFCEIYNENIHDLLEQHASNASRKTNLRLCQDVKGNSFIKDLKWVQVSSAEEALKVMKLGKRNQSISSTKLNLLSSRSHSIFSVRILKVEDVGIPRVESVSELALCDLAGSERCAKTQNKGERLKEAGNINTSLLSLGKCINALRNNQQARQHVPFRESKLTHYLQGYFTGRGSACMIVNINQCSSMYDETLNVLKFSAVAQKVVVLTTKNIPVGVKRSAREVSFIINNADCREMRRSSLVRWDPSLEDVQEDSDSEDEYEDQSEIEDTLLNSEDECTETVQINKKLYEGQQRLIVNTQQQLTAVQAELLTLEARVRDEVTKEFSELISQMQEDYSERMSRERELIEERCERRLEILKNLVEKTAMEDDRMNSTVAKSEEEKRVSLDSLFDSMSGDLAGIRHDAQAAQSCLVTSDPRVSATLHDLEKKVTELSEQLSISQNQLLLKTKELETQSRLSDELEEVKKTLESHREREFNLSDMCRQKDEMIAKLQATLDQHVERKAEDRAQMDSVQKEVIQCCCTIKEPLSSRNETRKRRQEGEGLEGQPPLKKGPPEDLKMPEDEADDGREDSALHEVKGQLECCLAEAKQKDKQITDLEQECHRLQVCIQDQKSNLDERVSQEHILKEDVCQTTLESKQMATAVDFSNLNVAVASRCSNTTGAFAAKSLMAKRDLELREKNKHILTLEKEIALMRQKAEHTSDYENLKKLNSELQAEVTSLCKVKAELEEKITCLQKEKEGLEDKEVGLTNPNTLGFKSKERDNQTHLMLSGKEAELALRENALTLKETQLSALQKSLKQTQERLEEEEMQAVQEARRKEVERRREILAVAEEAIAQKDAELQKRQVEISRLKEEIKTSEFKMSSLTVDLRRREDDSSDLREKLCDSKKQIQQVQKEISSMREAEKSLRLKLSDLEKTKVQLQNEIANRDRTINQLKAERSSGSKSDENLLLYQKACKDLQERERVIEDMRLALTEQEETQTQLDLELDNRDARIDELTQELASLREMLPEERDRSDSRPISKDVLQARQQITQAHESLQLANEKQQAERKKWMEEKLILIGQAKEAEERRNQDMRRFADDRERHVRQQAEMESLAARLNAREEEMERWRKERDTLVSALEVQLKKLIMSNGEKDQQIKMLQSSNTAQTPEEVSEDSRTEALQAELAVKEAEIQKLKEQLSISATNSSIRRNSTTQTMEVEFSDMKPLNSSTIKHKSRTRGSVISQNSSGSGPLVLDSSEISTETGRRSRFPRPELEISFSPLQPDRFALKRQGEESAVTVKISRPTRKRKSGEMDKRKGARSSAVKKKAFSEPNPQDAVEGENIRNTRCRLTPHLTPHREESPNSQESNQRGKKDKTLQKIGDFLQSSPTFLGSKAKRIMGLMSGKSPDGGSTPSLKLKSKRDKRKYDRPDISSPMDIPAHRIIGRNPEDKESENLIKKRLRTRMAK

Target ORF information:

RefSeq Version	XM_003200345.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 20Ba (kif20ba), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003200345.4

ORF Insert Sequence:

ATGATGGAGT CAGGGCTAAA GGATGATCCA GCAAGACCTG CTATATTAGT TGTAGAAGAT 
CTAAGGAGAA ATTTGTCCAC AGAGTTTGAT TGTTCAGATT TGCAGGACTC GTCAGACTTG 
AAGGAACATC TGAAGGTGTA TCTTAGGATA AGACCATTTT CAGCAGTTGA GATTGAGAAT 
GGAGAATCAC AGGACTGTGT GACCATTCAG CGTCCAGACA CTGTCCTTCT CAAACCTCCA 
CAAACATCTC TGTCTGCCAG GCTCAGTGAT AAATCAGTGC CAGTTACAGC TCAGCGTTTC 
CAGTTCTCTC AGGTCTATGG TCCAGACACT ACACAGAAGG ATATATTTGA TGGCACAGTA 
AACGGACTTG TCAGAAATGT TCTAGAAGGG CAAAATTCTC TGGTCTTTAC GTATGGTGTC 
ACCAATGCTG GGAAGACCTT TACTTTTTTA GGTCCAGACA CTAATCCGGG CATCTTGCCT 
CGTTCCCTCA ACATGATCTT CAGTAGTCTG GAGGGTCGCA TCTTTACCCA GATGTGCCTG 
AAACCCCAGA GGTGCCGAGA TTTCATCAGA CTTACAAAAG AGCAACAAAA TGAGGAAGCA 
GCCAGCAAAC GCAACCTGCT CAAGCTTTTC AAAGAGTCTG ATGCTCAGAG GAGTATTTCT 
GGCCATTGCT CAAAGTCTGA TATTGAAGAT GGTTCCATGC TCTCTGACGT AAACTCTGCG 
GTTTTGGATG TGGAGATGGA CATGCACACT AGGTTTTCTA TTTGGGTCTC GTTCTGTGAG 
ATCTACAATG AGAACATCCA TGACTTGCTA GAACAGCATG CAAGCAATGC TTCACGGAAA 
ACCAACCTTC GCCTTTGTCA GGATGTCAAA GGAAACTCAT TCATTAAAGA TCTAAAATGG 
GTCCAGGTGA GCAGTGCGGA GGAAGCCCTG AAAGTGATGA AACTGGGCAA AAGAAATCAG 
AGCATCTCTA GCACTAAACT CAACCTGCTC TCCAGCAGAA GCCACAGTAT TTTCTCTGTT 
CGAATACTGA AGGTGGAGGA TGTGGGAATA CCTAGAGTGG AGTCAGTCAG CGAGCTGGCA 
CTGTGTGATC TGGCTGGATC AGAGCGTTGT GCTAAGACTC AAAATAAAGG TGAACGGCTA 
AAAGAGGCTG GCAACATCAA TACTTCTCTG CTCAGTCTTG GAAAGTGCAT TAATGCACTA 
AGGAATAACC AACAGGCTAG ACAGCATGTT CCGTTCAGAG AGAGTAAACT CACTCACTAT 
CTGCAGGGCT ACTTCACAGG CCGGGGCTCA GCCTGTATGA TTGTAAACAT TAATCAGTGC 
TCCTCCATGT ATGATGAGAC GCTGAATGTG CTCAAGTTCT CTGCCGTGGC TCAGAAGGTG 
GTGGTCCTCA CCACTAAGAA CATCCCAGTG GGAGTGAAAA GGAGTGCAAG AGAGGTGTCC 
TTCATAATCA ACAACGCTGA TTGCCGGGAA ATGAGACGCA GCTCATTAGT ACGATGGGAC 
CCAAGTCTTG AGGATGTCCA GGAAGATTCT GACTCAGAGG ACGAGTATGA AGACCAGAGC 
GAAATCGAGG ACACCTTACT AAATTCTGAA GATGAATGTA CAGAGACTGT GCAAATCAAT 
AAAAAGCTTT ATGAGGGCCA GCAGCGGTTA ATAGTGAATA CTCAGCAGCA GTTGACAGCA 
GTACAAGCCG AACTCTTAAC CCTAGAAGCA CGTGTTCGAG ATGAAGTGAC AAAAGAATTC 
TCAGAACTGA TTTCCCAGAT GCAGGAAGAT TACAGTGAAC GTATGTCCAG AGAGCGGGAG 
CTGATAGAGG AACGGTGTGA ACGGAGACTG GAGATTCTCA AAAACCTAGT GGAAAAAACG 
GCTATGGAGG ATGACCGTAT GAATTCAACT GTGGCCAAAT CAGAGGAGGA GAAACGTGTC 
TCTCTAGACA GCCTGTTTGA CTCTATGAGT GGTGACCTGG CAGGAATCAG ACATGATGCC 
CAGGCTGCCC AGTCCTGCTT GGTGACCTCT GATCCCAGAG TCTCAGCGAC ATTGCATGAC 
CTTGAGAAAA AGGTCACAGA ACTTTCAGAA CAGCTCAGCA TATCTCAGAA TCAGCTGTTG 
CTCAAAACAA AGGAGCTTGA AACCCAGAGC CGGCTGTCAG ATGAACTGGA GGAGGTGAAG 
AAGACTCTGG AGAGCCACAG GGAAAGAGAA TTTAATCTGA GTGACATGTG CAGACAGAAG 
GATGAGATGA TTGCCAAACT CCAGGCTACT TTGGACCAGC ATGTAGAAAG AAAGGCTGAG 
GATAGAGCTC AAATGGACTC TGTCCAAAAG GAGGTCATCC AGTGCTGTTG CACCATTAAA 
GAGCCCCTCA GCTCCCGGAA TGAAACCAGA AAGCGACGTC AGGAAGGAGA GGGGCTTGAA 
GGACAGCCCC CGCTCAAGAA AGGCCCCCCG GAGGACTTGA AAATGCCTGA AGATGAAGCA 
GATGATGGGC GGGAAGACTC CGCCCTTCAT GAGGTCAAAG GTCAGCTGGA GTGTTGCCTT 
GCTGAAGCCA AACAAAAAGA CAAGCAGATC ACAGACCTTG AGCAGGAATG CCACAGATTA 
CAAGTGTGCA TTCAGGATCA AAAGTCAAAT CTGGATGAAA GAGTGTCACA GGAACATATT 
CTGAAAGAAG ACGTTTGTCA GACAACATTG GAAAGCAAAC AGATGGCAAC GGCTGTGGAT 
TTTAGCAATC TGAACGTAGC AGTTGCATCC AGATGTAGCA ACACTACAGG TGCGTTTGCT 
GCTAAATCAC TAATGGCTAA GCGAGATCTT GAACTGAGAG AAAAGAACAA ACACATCTTG 
ACTCTTGAAA AGGAAATTGC GCTAATGAGA CAGAAAGCAG AACACACATC TGACTATGAA 
AATCTCAAAA AACTCAACTC TGAACTGCAA GCTGAGGTCA CGAGCTTATG TAAGGTCAAA 
GCCGAACTTG AGGAGAAGAT TACATGCCTT CAGAAGGAGA AGGAGGGGCT TGAAGATAAG 
GAGGTGGGGC TGACAAACCC TAACACTCTT GGATTTAAAA GTAAAGAGAG AGATAACCAA 
ACACACCTCA TGCTGTCTGG AAAGGAGGCT GAACTTGCAC TGAGAGAGAA TGCTCTAACT 
TTAAAGGAAA CTCAGCTTTC AGCACTACAG AAGAGTCTGA AGCAAACACA GGAGCGTCTG 
GAGGAGGAGG AGATGCAGGC GGTGCAGGAG GCGCGCAGGA AGGAGGTGGA GAGGAGGAGA 
GAGATCCTGG CTGTGGCAGA GGAGGCCATC GCTCAGAAAG ATGCAGAGCT ACAGAAGAGA 
CAAGTAGAGA TCAGCCGGTT AAAAGAGGAG ATTAAAACTA GCGAGTTTAA GATGAGCAGT 
CTGACTGTTG ATCTGCGCAG AAGGGAAGAT GATTCATCTG ATCTTCGGGA AAAACTTTGT 
GACTCAAAGA AACAAATACA GCAGGTCCAG AAAGAGATTT CGTCCATGCG TGAAGCTGAG 
AAATCTCTAC GACTGAAGCT CAGCGATCTA GAAAAAACGA AAGTGCAGCT CCAGAATGAG 
ATCGCCAACA GAGATCGCAC CATTAACCAG TTAAAAGCTG AACGATCCTC TGGTTCAAAG 
TCAGATGAGA ATCTGCTGCT CTATCAGAAA GCATGTAAAG ATCTGCAAGA GCGTGAGCGT 
GTTATTGAAG ACATGCGCTT GGCTCTGACA GAACAGGAGG AAACACAGAC ACAGCTGGAC 
CTTGAGTTGG ACAACAGAGA CGCTCGGATT GATGAGCTCA CTCAAGAGCT GGCCAGTTTG 
AGAGAAATGT TGCCTGAAGA GAGGGACAGG AGTGATTCCA GACCAATCTC TAAAGATGTT 
TTACAGGCCA GACAGCAGAT CACTCAGGCC CACGAGAGCT TACAGCTTGC CAATGAGAAG 
CAGCAGGCAG AACGCAAGAA GTGGATGGAG GAGAAGTTGA TTCTGATTGG TCAGGCTAAA 
GAAGCTGAGG AGAGGCGGAA TCAGGACATG AGACGATTTG CAGATGATCG AGAACGGCAT 
GTTCGGCAAC AGGCTGAAAT GGAGTCTCTG GCTGCACGTC TTAATGCCCG TGAGGAAGAG 
ATGGAAAGGT GGAGAAAGGA GAGAGACACG CTTGTCTCTG CTCTGGAGGT TCAGCTGAAG 
AAACTCATCA TGTCCAATGG AGAAAAAGAC CAACAGATCA AGATGCTGCA GTCCAGTAAC 
ACTGCCCAAA CACCAGAGGA GGTCAGTGAG GACAGCAGGA CCGAGGCACT GCAGGCAGAG 
CTGGCTGTAA AAGAGGCTGA GATCCAGAAA CTGAAGGAGC AGCTCAGCAT CAGTGCCACA 
AACAGCAGCA TTCGCAGAAA CTCTACTACA CAGACGATGG AAGTGGAGTT TTCTGATATG 
AAGCCTCTGA ACAGCAGTAC AATCAAGCAC AAAAGCCGAA CCAGAGGATC TGTCATCAGT 
CAGAACTCAT CCGGTAGCGG TCCACTGGTG CTAGATTCAT CAGAGATCTC CACGGAAACA 
GGCAGACGGT CTAGGTTTCC AAGGCCTGAG CTGGAGATTT CCTTCAGTCC TCTACAGCCC 
GATCGCTTCG CTCTCAAGCG TCAGGGTGAA GAATCTGCTG TCACTGTTAA AATCTCCAGA 
CCCACACGCA AGAGGAAGAG CGGCGAGATG GACAAGAGGA AAGGAGCCAG GAGCAGCGCT 
GTGAAAAAGA AAGCTTTCTC TGAGCCCAAT CCACAGGATG CGGTGGAAGG TGAAAACATC 
AGAAATACAA GGTGCAGATT GACTCCTCAC CTAACACCTC ATCGGGAAGA GTCCCCAAAT 
TCCCAGGAGT CAAACCAAAG AGGGAAAAAA GACAAAACGC TCCAGAAGAT TGGAGATTTT 
CTTCAGAGCT CTCCCACCTT TCTTGGGAGT AAAGCGAAGA GGATCATGGG ATTGATGAGT 
GGAAAATCCC CTGATGGTGG GTCGACTCCT TCTCTAAAAC TGAAATCCAA ACGTGATAAA 
CGGAAATATG ACAGACCCGA TATCTCCTCA CCAATGGACA TACCGGCTCA TCGGATTATT 
GGCCGAAATC CAGAGGACAA AGAGAGTGAA AACCTTATCA AGAAACGTCT TCGTACAAGA 
ATGGCCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif20ba ( XM_003200345.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003200393.2 Danio rerio kinesin family member 20Ba (kif20ba), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa22505
Clone ID Related Accession (Same CDS sequence)	XM_021474376.1 , XM_003200345.4 , XM_003200345.5 , XM_021473144.1
Accession Version	XM_003200345.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF20B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003200345.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGT CAGGGCTAAA GGATGATCCA GCAAGACCTG CTATATTAGT TGTAGAAGAT 
CTAAGGAGAA ATTTGTCCAC AGAGTTTGAT TGTTCAGATT TGCAGGACTC GTCAGACTTG 
AAGGAACATC TGAAGGTGTA TCTTAGGATA AGACCATTTT CAGCAGTTGA GATTGAGAAT 
GGAGAATCAC AGGACTGTGT GACCATTCAG CGTCCAGACA CTGTCCTTCT CAAACCTCCA 
CAAACATCTC TGTCTGCCAG GCTCAGTGAT AAATCAGTGC CAGTTACAGC TCAGCGTTTC 
CAGTTCTCTC AGGTCTATGG TCCAGACACT ACACAGAAGG ATATATTTGA TGGCACAGTA 
AACGGACTTG TCAGAAATGT TCTAGAAGGG CAAAATTCTC TGGTCTTTAC GTATGGTGTC 
ACCAATGCTG GGAAGACCTT TACTTTTTTA GGTCCAGACA CTAATCCGGG CATCTTGCCT 
CGTTCCCTCA ACATGATCTT CAGTAGTCTG GAGGGTCGCA TCTTTACCCA GATGTGCCTG 
AAACCCCAGA GGTGCCGAGA TTTCATCAGA CTTACAAAAG AGCAACAAAA TGAGGAAGCA 
GCCAGCAAAC GCAACCTGCT CAAGCTTTTC AAAGAGTCTG ATGCTCAGAG GAGTATTTCT 
GGCCATTGCT CAAAGTCTGA TATTGAAGAT GGTTCCATGC TCTCTGACGT AAACTCTGCG 
GTTTTGGATG TGGAGATGGA CATGCACACT AGGTTTTCTA TTTGGGTCTC GTTCTGTGAG 
ATCTACAATG AGAACATCCA TGACTTGCTA GAACAGCATG CAAGCAATGC TTCACGGAAA 
ACCAACCTTC GCCTTTGTCA GGATGTCAAA GGAAACTCAT TCATTAAAGA TCTAAAATGG 
GTCCAGGTGA GCAGTGCGGA GGAAGCCCTG AAAGTGATGA AACTGGGCAA AAGAAATCAG 
AGCATCTCTA GCACTAAACT CAACCTGCTC TCCAGCAGAA GCCACAGTAT TTTCTCTGTT 
CGAATACTGA AGGTGGAGGA TGTGGGAATA CCTAGAGTGG AGTCAGTCAG CGAGCTGGCA 
CTGTGTGATC TGGCTGGATC AGAGCGTTGT GCTAAGACTC AAAATAAAGG TGAACGGCTA 
AAAGAGGCTG GCAACATCAA TACTTCTCTG CTCAGTCTTG GAAAGTGCAT TAATGCACTA 
AGGAATAACC AACAGGCTAG ACAGCATGTT CCGTTCAGAG AGAGTAAACT CACTCACTAT 
CTGCAGGGCT ACTTCACAGG CCGGGGCTCA GCCTGTATGA TTGTAAACAT TAATCAGTGC 
TCCTCCATGT ATGATGAGAC GCTGAATGTG CTCAAGTTCT CTGCCGTGGC TCAGAAGGTG 
GTGGTCCTCA CCACTAAGAA CATCCCAGTG GGAGTGAAAA GGAGTGCAAG AGAGGTGTCC 
TTCATAATCA ACAACGCTGA TTGCCGGGAA ATGAGACGCA GCTCATTAGT ACGATGGGAC 
CCAAGTCTTG AGGATGTCCA GGAAGATTCT GACTCAGAGG ACGAGTATGA AGACCAGAGC 
GAAATCGAGG ACACCTTACT AAATTCTGAA GATGAATGTA CAGAGACTGT GCAAATCAAT 
AAAAAGCTTT ATGAGGGCCA GCAGCGGTTA ATAGTGAATA CTCAGCAGCA GTTGACAGCA 
GTACAAGCCG AACTCTTAAC CCTAGAAGCA CGTGTTCGAG ATGAAGTGAC AAAAGAATTC 
TCAGAACTGA TTTCCCAGAT GCAGGAAGAT TACAGTGAAC GTATGTCCAG AGAGCGGGAG 
CTGATAGAGG AACGGTGTGA ACGGAGACTG GAGATTCTCA AAAACCTAGT GGAAAAAACG 
GCTATGGAGG ATGACCGTAT GAATTCAACT GTGGCCAAAT CAGAGGAGGA GAAACGTGTC 
TCTCTAGACA GCCTGTTTGA CTCTATGAGT GGTGACCTGG CAGGAATCAG ACATGATGCC 
CAGGCTGCCC AGTCCTGCTT GGTGACCTCT GATCCCAGAG TCTCAGCGAC ATTGCATGAC 
CTTGAGAAAA AGGTCACAGA ACTTTCAGAA CAGCTCAGCA TATCTCAGAA TCAGCTGTTG 
CTCAAAACAA AGGAGCTTGA AACCCAGAGC CGGCTGTCAG ATGAACTGGA GGAGGTGAAG 
AAGACTCTGG AGAGCCACAG GGAAAGAGAA TTTAATCTGA GTGACATGTG CAGACAGAAG 
GATGAGATGA TTGCCAAACT CCAGGCTACT TTGGACCAGC ATGTAGAAAG AAAGGCTGAG 
GATAGAGCTC AAATGGACTC TGTCCAAAAG GAGGTCATCC AGTGCTGTTG CACCATTAAA 
GAGCCCCTCA GCTCCCGGAA TGAAACCAGA AAGCGACGTC AGGAAGGAGA GGGGCTTGAA 
GGACAGCCCC CGCTCAAGAA AGGCCCCCCG GAGGACTTGA AAATGCCTGA AGATGAAGCA 
GATGATGGGC GGGAAGACTC CGCCCTTCAT GAGGTCAAAG GTCAGCTGGA GTGTTGCCTT 
GCTGAAGCCA AACAAAAAGA CAAGCAGATC ACAGACCTTG AGCAGGAATG CCACAGATTA 
CAAGTGTGCA TTCAGGATCA AAAGTCAAAT CTGGATGAAA GAGTGTCACA GGAACATATT 
CTGAAAGAAG ACGTTTGTCA GACAACATTG GAAAGCAAAC AGATGGCAAC GGCTGTGGAT 
TTTAGCAATC TGAACGTAGC AGTTGCATCC AGATGTAGCA ACACTACAGG TGCGTTTGCT 
GCTAAATCAC TAATGGCTAA GCGAGATCTT GAACTGAGAG AAAAGAACAA ACACATCTTG 
ACTCTTGAAA AGGAAATTGC GCTAATGAGA CAGAAAGCAG AACACACATC TGACTATGAA 
AATCTCAAAA AACTCAACTC TGAACTGCAA GCTGAGGTCA CGAGCTTATG TAAGGTCAAA 
GCCGAACTTG AGGAGAAGAT TACATGCCTT CAGAAGGAGA AGGAGGGGCT TGAAGATAAG 
GAGGTGGGGC TGACAAACCC TAACACTCTT GGATTTAAAA GTAAAGAGAG AGATAACCAA 
ACACACCTCA TGCTGTCTGG AAAGGAGGCT GAACTTGCAC TGAGAGAGAA TGCTCTAACT 
TTAAAGGAAA CTCAGCTTTC AGCACTACAG AAGAGTCTGA AGCAAACACA GGAGCGTCTG 
GAGGAGGAGG AGATGCAGGC GGTGCAGGAG GCGCGCAGGA AGGAGGTGGA GAGGAGGAGA 
GAGATCCTGG CTGTGGCAGA GGAGGCCATC GCTCAGAAAG ATGCAGAGCT ACAGAAGAGA 
CAAGTAGAGA TCAGCCGGTT AAAAGAGGAG ATTAAAACTA GCGAGTTTAA GATGAGCAGT 
CTGACTGTTG ATCTGCGCAG AAGGGAAGAT GATTCATCTG ATCTTCGGGA AAAACTTTGT 
GACTCAAAGA AACAAATACA GCAGGTCCAG AAAGAGATTT CGTCCATGCG TGAAGCTGAG 
AAATCTCTAC GACTGAAGCT CAGCGATCTA GAAAAAACGA AAGTGCAGCT CCAGAATGAG 
ATCGCCAACA GAGATCGCAC CATTAACCAG TTAAAAGCTG AACGATCCTC TGGTTCAAAG 
TCAGATGAGA ATCTGCTGCT CTATCAGAAA GCATGTAAAG ATCTGCAAGA GCGTGAGCGT 
GTTATTGAAG ACATGCGCTT GGCTCTGACA GAACAGGAGG AAACACAGAC ACAGCTGGAC 
CTTGAGTTGG ACAACAGAGA CGCTCGGATT GATGAGCTCA CTCAAGAGCT GGCCAGTTTG 
AGAGAAATGT TGCCTGAAGA GAGGGACAGG AGTGATTCCA GACCAATCTC TAAAGATGTT 
TTACAGGCCA GACAGCAGAT CACTCAGGCC CACGAGAGCT TACAGCTTGC CAATGAGAAG 
CAGCAGGCAG AACGCAAGAA GTGGATGGAG GAGAAGTTGA TTCTGATTGG TCAGGCTAAA 
GAAGCTGAGG AGAGGCGGAA TCAGGACATG AGACGATTTG CAGATGATCG AGAACGGCAT 
GTTCGGCAAC AGGCTGAAAT GGAGTCTCTG GCTGCACGTC TTAATGCCCG TGAGGAAGAG 
ATGGAAAGGT GGAGAAAGGA GAGAGACACG CTTGTCTCTG CTCTGGAGGT TCAGCTGAAG 
AAACTCATCA TGTCCAATGG AGAAAAAGAC CAACAGATCA AGATGCTGCA GTCCAGTAAC 
ACTGCCCAAA CACCAGAGGA GGTCAGTGAG GACAGCAGGA CCGAGGCACT GCAGGCAGAG 
CTGGCTGTAA AAGAGGCTGA GATCCAGAAA CTGAAGGAGC AGCTCAGCAT CAGTGCCACA 
AACAGCAGCA TTCGCAGAAA CTCTACTACA CAGACGATGG AAGTGGAGTT TTCTGATATG 
AAGCCTCTGA ACAGCAGTAC AATCAAGCAC AAAAGCCGAA CCAGAGGATC TGTCATCAGT 
CAGAACTCAT CCGGTAGCGG TCCACTGGTG CTAGATTCAT CAGAGATCTC CACGGAAACA 
GGCAGACGGT CTAGGTTTCC AAGGCCTGAG CTGGAGATTT CCTTCAGTCC TCTACAGCCC 
GATCGCTTCG CTCTCAAGCG TCAGGGTGAA GAATCTGCTG TCACTGTTAA AATCTCCAGA 
CCCACACGCA AGAGGAAGAG CGGCGAGATG GACAAGAGGA AAGGAGCCAG GAGCAGCGCT 
GTGAAAAAGA AAGCTTTCTC TGAGCCCAAT CCACAGGATG CGGTGGAAGG TGAAAACATC 
AGAAATACAA GGTGCAGATT GACTCCTCAC CTAACACCTC ATCGGGAAGA GTCCCCAAAT 
TCCCAGGAGT CAAACCAAAG AGGGAAAAAA GACAAAACGC TCCAGAAGAT TGGAGATTTT 
CTTCAGAGCT CTCCCACCTT TCTTGGGAGT AAAGCGAAGA GGATCATGGG ATTGATGAGT 
GGAAAATCCC CTGATGGTGG GTCGACTCCT TCTCTAAAAC TGAAATCCAA ACGTGATAAA 
CGGAAATATG ACAGACCCGA TATCTCCTCA CCAATGGACA TACCGGCTCA TCGGATTATT 
GGCCGAAATC CAGAGGACAA AGAGAGTGAA AACCTTATCA AGAAACGTCT TCGTACAAGA 
ATGGCCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003200393.2
CDS	149..5260
Translation	MMESGLKDDPARPAILVVEDLRRNLSTEFDCSDLQDSSDLKEHLKVYLRIRPFSAVEIENGESQDCVTIQRPDTVLLKPPQTSLSARLSDKSVPVTAQRFQFSQVYGPDTTQKDIFDGTVNGLVRNVLEGQNSLVFTYGVTNAGKTFTFLGPDTNPGILPRSLNMIFSSLEGRIFTQMCLKPQRCRDFIRLTKEQQNEEAASKRNLLKLFKESDAQRSISGHCSKSDIEDGSMLSDVNSAVLDVEMDMHTRFSIWVSFCEIYNENIHDLLEQHASNASRKTNLRLCQDVKGNSFIKDLKWVQVSSAEEALKVMKLGKRNQSISSTKLNLLSSRSHSIFSVRILKVEDVGIPRVESVSELALCDLAGSERCAKTQNKGERLKEAGNINTSLLSLGKCINALRNNQQARQHVPFRESKLTHYLQGYFTGRGSACMIVNINQCSSMYDETLNVLKFSAVAQKVVVLTTKNIPVGVKRSAREVSFIINNADCREMRRSSLVRWDPSLEDVQEDSDSEDEYEDQSEIEDTLLNSEDECTETVQINKKLYEGQQRLIVNTQQQLTAVQAELLTLEARVRDEVTKEFSELISQMQEDYSERMSRERELIEERCERRLEILKNLVEKTAMEDDRMNSTVAKSEEEKRVSLDSLFDSMSGDLAGIRHDAQAAQSCLVTSDPRVSATLHDLEKKVTELSEQLSISQNQLLLKTKELETQSRLSDELEEVKKTLESHREREFNLSDMCRQKDEMIAKLQATLDQHVERKAEDRAQMDSVQKEVIQCCCTIKEPLSSRNETRKRRQEGEGLEGQPPLKKGPPEDLKMPEDEADDGREDSALHEVKGQLECCLAEAKQKDKQITDLEQECHRLQVCIQDQKSNLDERVSQEHILKEDVCQTTLESKQMATAVDFSNLNVAVASRCSNTTGAFAAKSLMAKRDLELREKNKHILTLEKEIALMRQKAEHTSDYENLKKLNSELQAEVTSLCKVKAELEEKITCLQKEKEGLEDKEVGLTNPNTLGFKSKERDNQTHLMLSGKEAELALRENALTLKETQLSALQKSLKQTQERLEEEEMQAVQEARRKEVERRREILAVAEEAIAQKDAELQKRQVEISRLKEEIKTSEFKMSSLTVDLRRREDDSSDLREKLCDSKKQIQQVQKEISSMREAEKSLRLKLSDLEKTKVQLQNEIANRDRTINQLKAERSSGSKSDENLLLYQKACKDLQERERVIEDMRLALTEQEETQTQLDLELDNRDARIDELTQELASLREMLPEERDRSDSRPISKDVLQARQQITQAHESLQLANEKQQAERKKWMEEKLILIGQAKEAEERRNQDMRRFADDRERHVRQQAEMESLAARLNAREEEMERWRKERDTLVSALEVQLKKLIMSNGEKDQQIKMLQSSNTAQTPEEVSEDSRTEALQAELAVKEAEIQKLKEQLSISATNSSIRRNSTTQTMEVEFSDMKPLNSSTIKHKSRTRGSVISQNSSGSGPLVLDSSEISTETGRRSRFPRPELEISFSPLQPDRFALKRQGEESAVTVKISRPTRKRKSGEMDKRKGARSSAVKKKAFSEPNPQDAVEGENIRNTRCRLTPHLTPHREESPNSQESNQRGKKDKTLQKIGDFLQSSPTFLGSKAKRIMGLMSGKSPDGGSTPSLKLKSKRDKRKYDRPDISSPMDIPAHRIIGRNPEDKESENLIKKRLRTRMAK

Target ORF information:

RefSeq Version	XM_003200345.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 20Ba (kif20ba), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003200345.5

ORF Insert Sequence:

ATGATGGAGT CAGGGCTAAA GGATGATCCA GCAAGACCTG CTATATTAGT TGTAGAAGAT 
CTAAGGAGAA ATTTGTCCAC AGAGTTTGAT TGTTCAGATT TGCAGGACTC GTCAGACTTG 
AAGGAACATC TGAAGGTGTA TCTTAGGATA AGACCATTTT CAGCAGTTGA GATTGAGAAT 
GGAGAATCAC AGGACTGTGT GACCATTCAG CGTCCAGACA CTGTCCTTCT CAAACCTCCA 
CAAACATCTC TGTCTGCCAG GCTCAGTGAT AAATCAGTGC CAGTTACAGC TCAGCGTTTC 
CAGTTCTCTC AGGTCTATGG TCCAGACACT ACACAGAAGG ATATATTTGA TGGCACAGTA 
AACGGACTTG TCAGAAATGT TCTAGAAGGG CAAAATTCTC TGGTCTTTAC GTATGGTGTC 
ACCAATGCTG GGAAGACCTT TACTTTTTTA GGTCCAGACA CTAATCCGGG CATCTTGCCT 
CGTTCCCTCA ACATGATCTT CAGTAGTCTG GAGGGTCGCA TCTTTACCCA GATGTGCCTG 
AAACCCCAGA GGTGCCGAGA TTTCATCAGA CTTACAAAAG AGCAACAAAA TGAGGAAGCA 
GCCAGCAAAC GCAACCTGCT CAAGCTTTTC AAAGAGTCTG ATGCTCAGAG GAGTATTTCT 
GGCCATTGCT CAAAGTCTGA TATTGAAGAT GGTTCCATGC TCTCTGACGT AAACTCTGCG 
GTTTTGGATG TGGAGATGGA CATGCACACT AGGTTTTCTA TTTGGGTCTC GTTCTGTGAG 
ATCTACAATG AGAACATCCA TGACTTGCTA GAACAGCATG CAAGCAATGC TTCACGGAAA 
ACCAACCTTC GCCTTTGTCA GGATGTCAAA GGAAACTCAT TCATTAAAGA TCTAAAATGG 
GTCCAGGTGA GCAGTGCGGA GGAAGCCCTG AAAGTGATGA AACTGGGCAA AAGAAATCAG 
AGCATCTCTA GCACTAAACT CAACCTGCTC TCCAGCAGAA GCCACAGTAT TTTCTCTGTT 
CGAATACTGA AGGTGGAGGA TGTGGGAATA CCTAGAGTGG AGTCAGTCAG CGAGCTGGCA 
CTGTGTGATC TGGCTGGATC AGAGCGTTGT GCTAAGACTC AAAATAAAGG TGAACGGCTA 
AAAGAGGCTG GCAACATCAA TACTTCTCTG CTCAGTCTTG GAAAGTGCAT TAATGCACTA 
AGGAATAACC AACAGGCTAG ACAGCATGTT CCGTTCAGAG AGAGTAAACT CACTCACTAT 
CTGCAGGGCT ACTTCACAGG CCGGGGCTCA GCCTGTATGA TTGTAAACAT TAATCAGTGC 
TCCTCCATGT ATGATGAGAC GCTGAATGTG CTCAAGTTCT CTGCCGTGGC TCAGAAGGTG 
GTGGTCCTCA CCACTAAGAA CATCCCAGTG GGAGTGAAAA GGAGTGCAAG AGAGGTGTCC 
TTCATAATCA ACAACGCTGA TTGCCGGGAA ATGAGACGCA GCTCATTAGT ACGATGGGAC 
CCAAGTCTTG AGGATGTCCA GGAAGATTCT GACTCAGAGG ACGAGTATGA AGACCAGAGC 
GAAATCGAGG ACACCTTACT AAATTCTGAA GATGAATGTA CAGAGACTGT GCAAATCAAT 
AAAAAGCTTT ATGAGGGCCA GCAGCGGTTA ATAGTGAATA CTCAGCAGCA GTTGACAGCA 
GTACAAGCCG AACTCTTAAC CCTAGAAGCA CGTGTTCGAG ATGAAGTGAC AAAAGAATTC 
TCAGAACTGA TTTCCCAGAT GCAGGAAGAT TACAGTGAAC GTATGTCCAG AGAGCGGGAG 
CTGATAGAGG AACGGTGTGA ACGGAGACTG GAGATTCTCA AAAACCTAGT GGAAAAAACG 
GCTATGGAGG ATGACCGTAT GAATTCAACT GTGGCCAAAT CAGAGGAGGA GAAACGTGTC 
TCTCTAGACA GCCTGTTTGA CTCTATGAGT GGTGACCTGG CAGGAATCAG ACATGATGCC 
CAGGCTGCCC AGTCCTGCTT GGTGACCTCT GATCCCAGAG TCTCAGCGAC ATTGCATGAC 
CTTGAGAAAA AGGTCACAGA ACTTTCAGAA CAGCTCAGCA TATCTCAGAA TCAGCTGTTG 
CTCAAAACAA AGGAGCTTGA AACCCAGAGC CGGCTGTCAG ATGAACTGGA GGAGGTGAAG 
AAGACTCTGG AGAGCCACAG GGAAAGAGAA TTTAATCTGA GTGACATGTG CAGACAGAAG 
GATGAGATGA TTGCCAAACT CCAGGCTACT TTGGACCAGC ATGTAGAAAG AAAGGCTGAG 
GATAGAGCTC AAATGGACTC TGTCCAAAAG GAGGTCATCC AGTGCTGTTG CACCATTAAA 
GAGCCCCTCA GCTCCCGGAA TGAAACCAGA AAGCGACGTC AGGAAGGAGA GGGGCTTGAA 
GGACAGCCCC CGCTCAAGAA AGGCCCCCCG GAGGACTTGA AAATGCCTGA AGATGAAGCA 
GATGATGGGC GGGAAGACTC CGCCCTTCAT GAGGTCAAAG GTCAGCTGGA GTGTTGCCTT 
GCTGAAGCCA AACAAAAAGA CAAGCAGATC ACAGACCTTG AGCAGGAATG CCACAGATTA 
CAAGTGTGCA TTCAGGATCA AAAGTCAAAT CTGGATGAAA GAGTGTCACA GGAACATATT 
CTGAAAGAAG ACGTTTGTCA GACAACATTG GAAAGCAAAC AGATGGCAAC GGCTGTGGAT 
TTTAGCAATC TGAACGTAGC AGTTGCATCC AGATGTAGCA ACACTACAGG TGCGTTTGCT 
GCTAAATCAC TAATGGCTAA GCGAGATCTT GAACTGAGAG AAAAGAACAA ACACATCTTG 
ACTCTTGAAA AGGAAATTGC GCTAATGAGA CAGAAAGCAG AACACACATC TGACTATGAA 
AATCTCAAAA AACTCAACTC TGAACTGCAA GCTGAGGTCA CGAGCTTATG TAAGGTCAAA 
GCCGAACTTG AGGAGAAGAT TACATGCCTT CAGAAGGAGA AGGAGGGGCT TGAAGATAAG 
GAGGTGGGGC TGACAAACCC TAACACTCTT GGATTTAAAA GTAAAGAGAG AGATAACCAA 
ACACACCTCA TGCTGTCTGG AAAGGAGGCT GAACTTGCAC TGAGAGAGAA TGCTCTAACT 
TTAAAGGAAA CTCAGCTTTC AGCACTACAG AAGAGTCTGA AGCAAACACA GGAGCGTCTG 
GAGGAGGAGG AGATGCAGGC GGTGCAGGAG GCGCGCAGGA AGGAGGTGGA GAGGAGGAGA 
GAGATCCTGG CTGTGGCAGA GGAGGCCATC GCTCAGAAAG ATGCAGAGCT ACAGAAGAGA 
CAAGTAGAGA TCAGCCGGTT AAAAGAGGAG ATTAAAACTA GCGAGTTTAA GATGAGCAGT 
CTGACTGTTG ATCTGCGCAG AAGGGAAGAT GATTCATCTG ATCTTCGGGA AAAACTTTGT 
GACTCAAAGA AACAAATACA GCAGGTCCAG AAAGAGATTT CGTCCATGCG TGAAGCTGAG 
AAATCTCTAC GACTGAAGCT CAGCGATCTA GAAAAAACGA AAGTGCAGCT CCAGAATGAG 
ATCGCCAACA GAGATCGCAC CATTAACCAG TTAAAAGCTG AACGATCCTC TGGTTCAAAG 
TCAGATGAGA ATCTGCTGCT CTATCAGAAA GCATGTAAAG ATCTGCAAGA GCGTGAGCGT 
GTTATTGAAG ACATGCGCTT GGCTCTGACA GAACAGGAGG AAACACAGAC ACAGCTGGAC 
CTTGAGTTGG ACAACAGAGA CGCTCGGATT GATGAGCTCA CTCAAGAGCT GGCCAGTTTG 
AGAGAAATGT TGCCTGAAGA GAGGGACAGG AGTGATTCCA GACCAATCTC TAAAGATGTT 
TTACAGGCCA GACAGCAGAT CACTCAGGCC CACGAGAGCT TACAGCTTGC CAATGAGAAG 
CAGCAGGCAG AACGCAAGAA GTGGATGGAG GAGAAGTTGA TTCTGATTGG TCAGGCTAAA 
GAAGCTGAGG AGAGGCGGAA TCAGGACATG AGACGATTTG CAGATGATCG AGAACGGCAT 
GTTCGGCAAC AGGCTGAAAT GGAGTCTCTG GCTGCACGTC TTAATGCCCG TGAGGAAGAG 
ATGGAAAGGT GGAGAAAGGA GAGAGACACG CTTGTCTCTG CTCTGGAGGT TCAGCTGAAG 
AAACTCATCA TGTCCAATGG AGAAAAAGAC CAACAGATCA AGATGCTGCA GTCCAGTAAC 
ACTGCCCAAA CACCAGAGGA GGTCAGTGAG GACAGCAGGA CCGAGGCACT GCAGGCAGAG 
CTGGCTGTAA AAGAGGCTGA GATCCAGAAA CTGAAGGAGC AGCTCAGCAT CAGTGCCACA 
AACAGCAGCA TTCGCAGAAA CTCTACTACA CAGACGATGG AAGTGGAGTT TTCTGATATG 
AAGCCTCTGA ACAGCAGTAC AATCAAGCAC AAAAGCCGAA CCAGAGGATC TGTCATCAGT 
CAGAACTCAT CCGGTAGCGG TCCACTGGTG CTAGATTCAT CAGAGATCTC CACGGAAACA 
GGCAGACGGT CTAGGTTTCC AAGGCCTGAG CTGGAGATTT CCTTCAGTCC TCTACAGCCC 
GATCGCTTCG CTCTCAAGCG TCAGGGTGAA GAATCTGCTG TCACTGTTAA AATCTCCAGA 
CCCACACGCA AGAGGAAGAG CGGCGAGATG GACAAGAGGA AAGGAGCCAG GAGCAGCGCT 
GTGAAAAAGA AAGCTTTCTC TGAGCCCAAT CCACAGGATG CGGTGGAAGG TGAAAACATC 
AGAAATACAA GGTGCAGATT GACTCCTCAC CTAACACCTC ATCGGGAAGA GTCCCCAAAT 
TCCCAGGAGT CAAACCAAAG AGGGAAAAAA GACAAAACGC TCCAGAAGAT TGGAGATTTT 
CTTCAGAGCT CTCCCACCTT TCTTGGGAGT AAAGCGAAGA GGATCATGGG ATTGATGAGT 
GGAAAATCCC CTGATGGTGG GTCGACTCCT TCTCTAAAAC TGAAATCCAA ACGTGATAAA 
CGGAAATATG ACAGACCCGA TATCTCCTCA CCAATGGACA TACCGGCTCA TCGGATTATT 
GGCCGAAATC CAGAGGACAA AGAGAGTGAA AACCTTATCA AGAAACGTCT TCGTACAAGA 
ATGGCCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif20ba ( XM_021473144.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328819.1 Danio rerio kinesin family member 20Ba (kif20ba), transcript variant X1, mRNA.

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CloneID	ODa22505
Clone ID Related Accession (Same CDS sequence)	XM_021474376.1 , XM_003200345.4 , XM_003200345.5 , XM_021473144.1
Accession Version	XM_021473144.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF20B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394988.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGT CAGGGCTAAA GGATGATCCA GCAAGACCTG CTATATTAGT TGTAGAAGAT 
CTAAGGAGAA ATTTGTCCAC AGAGTTTGAT TGTTCAGATT TGCAGGACTC GTCAGACTTG 
AAGGAACATC TGAAGGTGTA TCTTAGGATA AGACCATTTT CAGCAGTTGA GATTGAGAAT 
GGAGAATCAC AGGACTGTGT GACCATTCAG CGTCCAGACA CTGTCCTTCT CAAACCTCCA 
CAAACATCTC TGTCTGCCAG GCTCAGTGAT AAATCAGTGC CAGTTACAGC TCAGCGTTTC 
CAGTTCTCTC AGGTCTATGG TCCAGACACT ACACAGAAGG ATATATTTGA TGGCACAGTA 
AACGGACTTG TCAGAAATGT TCTAGAAGGG CAAAATTCTC TGGTCTTTAC GTATGGTGTC 
ACCAATGCTG GGAAGACCTT TACTTTTTTA GGTCCAGACA CTAATCCGGG CATCTTGCCT 
CGTTCCCTCA ACATGATCTT CAGTAGTCTG GAGGGTCGCA TCTTTACCCA GATGTGCCTG 
AAACCCCAGA GGTGCCGAGA TTTCATCAGA CTTACAAAAG AGCAACAAAA TGAGGAAGCA 
GCCAGCAAAC GCAACCTGCT CAAGCTTTTC AAAGAGTCTG ATGCTCAGAG GAGTATTTCT 
GGCCATTGCT CAAAGTCTGA TATTGAAGAT GGTTCCATGC TCTCTGACGT AAACTCTGCG 
GTTTTGGATG TGGAGATGGA CATGCACACT AGGTTTTCTA TTTGGGTCTC GTTCTGTGAG 
ATCTACAATG AGAACATCCA TGACTTGCTA GAACAGCATG CAAGCAATGC TTCACGGAAA 
ACCAACCTTC GCCTTTGTCA GGATGTCAAA GGAAACTCAT TCATTAAAGA TCTAAAATGG 
GTCCAGGTGA GCAGTGCGGA GGAAGCCCTG AAAGTGATGA AACTGGGCAA AAGAAATCAG 
AGCATCTCTA GCACTAAACT CAACCTGCTC TCCAGCAGAA GCCACAGTAT TTTCTCTGTT 
CGAATACTGA AGGTGGAGGA TGTGGGAATA CCTAGAGTGG AGTCAGTCAG CGAGCTGGCA 
CTGTGTGATC TGGCTGGATC AGAGCGTTGT GCTAAGACTC AAAATAAAGG TGAACGGCTA 
AAAGAGGCTG GCAACATCAA TACTTCTCTG CTCAGTCTTG GAAAGTGCAT TAATGCACTA 
AGGAATAACC AACAGGCTAG ACAGCATGTT CCGTTCAGAG AGAGTAAACT CACTCACTAT 
CTGCAGGGCT ACTTCACAGG CCGGGGCTCA GCCTGTATGA TTGTAAACAT TAATCAGTGC 
TCCTCCATGT ATGATGAGAC GCTGAATGTG CTCAAGTTCT CTGCCGTGGC TCAGAAGGTG 
GTGGTCCTCA CCACTAAGAA CATCCCAGTG GGAGTGAAAA GGAGTGCAAG AGAGGTGTCC 
TTCATAATCA ACAACGCTGA TTGCCGGGAA ATGAGACGCA GCTCATTAGT ACGATGGGAC 
CCAAGTCTTG AGGATGTCCA GGAAGATTCT GACTCAGAGG ACGAGTATGA AGACCAGAGC 
GAAATCGAGG ACACCTTACT AAATTCTGAA GATGAATGTA CAGAGACTGT GCAAATCAAT 
AAAAAGCTTT ATGAGGGCCA GCAGCGGTTA ATAGTGAATA CTCAGCAGCA GTTGACAGCA 
GTACAAGCCG AACTCTTAAC CCTAGAAGCA CGTGTTCGAG ATGAAGTGAC AAAAGAATTC 
TCAGAACTGA TTTCCCAGAT GCAGGAAGAT TACAGTGAAC GTATGTCCAG AGAGCGGGAG 
CTGATAGAGG AACGGTGTGA ACGGAGACTG GAGATTCTCA AAAACCTAGT GGAAAAAACG 
GCTATGGAGG ATGACCGTAT GAATTCAACT GTGGCCAAAT CAGAGGAGGA GAAACGTGTC 
TCTCTAGACA GCCTGTTTGA CTCTATGAGT GGTGACCTGG CAGGAATCAG ACATGATGCC 
CAGGCTGCCC AGTCCTGCTT GGTGACCTCT GATCCCAGAG TCTCAGCGAC ATTGCATGAC 
CTTGAGAAAA AGGTCACAGA ACTTTCAGAA CAGCTCAGCA TATCTCAGAA TCAGCTGTTG 
CTCAAAACAA AGGAGCTTGA AACCCAGAGC CGGCTGTCAG ATGAACTGGA GGAGGTGAAG 
AAGACTCTGG AGAGCCACAG GGAAAGAGAA TTTAATCTGA GTGACATGTG CAGACAGAAG 
GATGAGATGA TTGCCAAACT CCAGGCTACT TTGGACCAGC ATGTAGAAAG AAAGGCTGAG 
GATAGAGCTC AAATGGACTC TGTCCAAAAG GAGGTCATCC AGTGCTGTTG CACCATTAAA 
GAGCCCCTCA GCTCCCGGAA TGAAACCAGA AAGCGACGTC AGGAAGGAGA GGGGCTTGAA 
GGACAGCCCC CGCTCAAGAA AGGCCCCCCG GAGGACTTGA AAATGCCTGA AGATGAAGCA 
GATGATGGGC GGGAAGACTC CGCCCTTCAT GAGGTCAAAG GTCAGCTGGA GTGTTGCCTT 
GCTGAAGCCA AACAAAAAGA CAAGCAGATC ACAGACCTTG AGCAGGAATG CCACAGATTA 
CAAGTGTGCA TTCAGGATCA AAAGTCAAAT CTGGATGAAA GAGTGTCACA GGAACATATT 
CTGAAAGAAG ACGTTTGTCA GACAACATTG GAAAGCAAAC AGATGGCAAC GGCTGTGGAT 
TTTAGCAATC TGAACGTAGC AGTTGCATCC AGATGTAGCA ACACTACAGG TGCGTTTGCT 
GCTAAATCAC TAATGGCTAA GCGAGATCTT GAACTGAGAG AAAAGAACAA ACACATCTTG 
ACTCTTGAAA AGGAAATTGC GCTAATGAGA CAGAAAGCAG AACACACATC TGACTATGAA 
AATCTCAAAA AACTCAACTC TGAACTGCAA GCTGAGGTCA CGAGCTTATG TAAGGTCAAA 
GCCGAACTTG AGGAGAAGAT TACATGCCTT CAGAAGGAGA AGGAGGGGCT TGAAGATAAG 
GAGGTGGGGC TGACAAACCC TAACACTCTT GGATTTAAAA GTAAAGAGAG AGATAACCAA 
ACACACCTCA TGCTGTCTGG AAAGGAGGCT GAACTTGCAC TGAGAGAGAA TGCTCTAACT 
TTAAAGGAAA CTCAGCTTTC AGCACTACAG AAGAGTCTGA AGCAAACACA GGAGCGTCTG 
GAGGAGGAGG AGATGCAGGC GGTGCAGGAG GCGCGCAGGA AGGAGGTGGA GAGGAGGAGA 
GAGATCCTGG CTGTGGCAGA GGAGGCCATC GCTCAGAAAG ATGCAGAGCT ACAGAAGAGA 
CAAGTAGAGA TCAGCCGGTT AAAAGAGGAG ATTAAAACTA GCGAGTTTAA GATGAGCAGT 
CTGACTGTTG ATCTGCGCAG AAGGGAAGAT GATTCATCTG ATCTTCGGGA AAAACTTTGT 
GACTCAAAGA AACAAATACA GCAGGTCCAG AAAGAGATTT CGTCCATGCG TGAAGCTGAG 
AAATCTCTAC GACTGAAGCT CAGCGATCTA GAAAAAACGA AAGTGCAGCT CCAGAATGAG 
ATCGCCAACA GAGATCGCAC CATTAACCAG TTAAAAGCTG AACGATCCTC TGGTTCAAAG 
TCAGATGAGA ATCTGCTGCT CTATCAGAAA GCATGTAAAG ATCTGCAAGA GCGTGAGCGT 
GTTATTGAAG ACATGCGCTT GGCTCTGACA GAACAGGAGG AAACACAGAC ACAGCTGGAC 
CTTGAGTTGG ACAACAGAGA CGCTCGGATT GATGAGCTCA CTCAAGAGCT GGCCAGTTTG 
AGAGAAATGT TGCCTGAAGA GAGGGACAGG AGTGATTCCA GACCAATCTC TAAAGATGTT 
TTACAGGCCA GACAGCAGAT CACTCAGGCC CACGAGAGCT TACAGCTTGC CAATGAGAAG 
CAGCAGGCAG AACGCAAGAA GTGGATGGAG GAGAAGTTGA TTCTGATTGG TCAGGCTAAA 
GAAGCTGAGG AGAGGCGGAA TCAGGACATG AGACGATTTG CAGATGATCG AGAACGGCAT 
GTTCGGCAAC AGGCTGAAAT GGAGTCTCTG GCTGCACGTC TTAATGCCCG TGAGGAAGAG 
ATGGAAAGGT GGAGAAAGGA GAGAGACACG CTTGTCTCTG CTCTGGAGGT TCAGCTGAAG 
AAACTCATCA TGTCCAATGG AGAAAAAGAC CAACAGATCA AGATGCTGCA GTCCAGTAAC 
ACTGCCCAAA CACCAGAGGA GGTCAGTGAG GACAGCAGGA CCGAGGCACT GCAGGCAGAG 
CTGGCTGTAA AAGAGGCTGA GATCCAGAAA CTGAAGGAGC AGCTCAGCAT CAGTGCCACA 
AACAGCAGCA TTCGCAGAAA CTCTACTACA CAGACGATGG AAGTGGAGTT TTCTGATATG 
AAGCCTCTGA ACAGCAGTAC AATCAAGCAC AAAAGCCGAA CCAGAGGATC TGTCATCAGT 
CAGAACTCAT CCGGTAGCGG TCCACTGGTG CTAGATTCAT CAGAGATCTC CACGGAAACA 
GGCAGACGGT CTAGGTTTCC AAGGCCTGAG CTGGAGATTT CCTTCAGTCC TCTACAGCCC 
GATCGCTTCG CTCTCAAGCG TCAGGGTGAA GAATCTGCTG TCACTGTTAA AATCTCCAGA 
CCCACACGCA AGAGGAAGAG CGGCGAGATG GACAAGAGGA AAGGAGCCAG GAGCAGCGCT 
GTGAAAAAGA AAGCTTTCTC TGAGCCCAAT CCACAGGATG CGGTGGAAGG TGAAAACATC 
AGAAATACAA GGTGCAGATT GACTCCTCAC CTAACACCTC ATCGGGAAGA GTCCCCAAAT 
TCCCAGGAGT CAAACCAAAG AGGGAAAAAA GACAAAACGC TCCAGAAGAT TGGAGATTTT 
CTTCAGAGCT CTCCCACCTT TCTTGGGAGT AAAGCGAAGA GGATCATGGG ATTGATGAGT 
GGAAAATCCC CTGATGGTGG GTCGACTCCT TCTCTAAAAC TGAAATCCAA ACGTGATAAA 
CGGAAATATG ACAGACCCGA TATCTCCTCA CCAATGGACA TACCGGCTCA TCGGATTATT 
GGCCGAAATC CAGAGGACAA AGAGAGTGAA AACCTTATCA AGAAACGTCT TCGTACAAGA 
ATGGCCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328819.1
CDS	149..5260
Translation	MMESGLKDDPARPAILVVEDLRRNLSTEFDCSDLQDSSDLKEHLKVYLRIRPFSAVEIENGESQDCVTIQRPDTVLLKPPQTSLSARLSDKSVPVTAQRFQFSQVYGPDTTQKDIFDGTVNGLVRNVLEGQNSLVFTYGVTNAGKTFTFLGPDTNPGILPRSLNMIFSSLEGRIFTQMCLKPQRCRDFIRLTKEQQNEEAASKRNLLKLFKESDAQRSISGHCSKSDIEDGSMLSDVNSAVLDVEMDMHTRFSIWVSFCEIYNENIHDLLEQHASNASRKTNLRLCQDVKGNSFIKDLKWVQVSSAEEALKVMKLGKRNQSISSTKLNLLSSRSHSIFSVRILKVEDVGIPRVESVSELALCDLAGSERCAKTQNKGERLKEAGNINTSLLSLGKCINALRNNQQARQHVPFRESKLTHYLQGYFTGRGSACMIVNINQCSSMYDETLNVLKFSAVAQKVVVLTTKNIPVGVKRSAREVSFIINNADCREMRRSSLVRWDPSLEDVQEDSDSEDEYEDQSEIEDTLLNSEDECTETVQINKKLYEGQQRLIVNTQQQLTAVQAELLTLEARVRDEVTKEFSELISQMQEDYSERMSRERELIEERCERRLEILKNLVEKTAMEDDRMNSTVAKSEEEKRVSLDSLFDSMSGDLAGIRHDAQAAQSCLVTSDPRVSATLHDLEKKVTELSEQLSISQNQLLLKTKELETQSRLSDELEEVKKTLESHREREFNLSDMCRQKDEMIAKLQATLDQHVERKAEDRAQMDSVQKEVIQCCCTIKEPLSSRNETRKRRQEGEGLEGQPPLKKGPPEDLKMPEDEADDGREDSALHEVKGQLECCLAEAKQKDKQITDLEQECHRLQVCIQDQKSNLDERVSQEHILKEDVCQTTLESKQMATAVDFSNLNVAVASRCSNTTGAFAAKSLMAKRDLELREKNKHILTLEKEIALMRQKAEHTSDYENLKKLNSELQAEVTSLCKVKAELEEKITCLQKEKEGLEDKEVGLTNPNTLGFKSKERDNQTHLMLSGKEAELALRENALTLKETQLSALQKSLKQTQERLEEEEMQAVQEARRKEVERRREILAVAEEAIAQKDAELQKRQVEISRLKEEIKTSEFKMSSLTVDLRRREDDSSDLREKLCDSKKQIQQVQKEISSMREAEKSLRLKLSDLEKTKVQLQNEIANRDRTINQLKAERSSGSKSDENLLLYQKACKDLQERERVIEDMRLALTEQEETQTQLDLELDNRDARIDELTQELASLREMLPEERDRSDSRPISKDVLQARQQITQAHESLQLANEKQQAERKKWMEEKLILIGQAKEAEERRNQDMRRFADDRERHVRQQAEMESLAARLNAREEEMERWRKERDTLVSALEVQLKKLIMSNGEKDQQIKMLQSSNTAQTPEEVSEDSRTEALQAELAVKEAEIQKLKEQLSISATNSSIRRNSTTQTMEVEFSDMKPLNSSTIKHKSRTRGSVISQNSSGSGPLVLDSSEISTETGRRSRFPRPELEISFSPLQPDRFALKRQGEESAVTVKISRPTRKRKSGEMDKRKGARSSAVKKKAFSEPNPQDAVEGENIRNTRCRLTPHLTPHREESPNSQESNQRGKKDKTLQKIGDFLQSSPTFLGSKAKRIMGLMSGKSPDGGSTPSLKLKSKRDKRKYDRPDISSPMDIPAHRIIGRNPEDKESENLIKKRLRTRMAK

Target ORF information:

RefSeq Version	XM_021473144.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 20Ba (kif20ba), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473144.1

ORF Insert Sequence:

ATGATGGAGT CAGGGCTAAA GGATGATCCA GCAAGACCTG CTATATTAGT TGTAGAAGAT 
CTAAGGAGAA ATTTGTCCAC AGAGTTTGAT TGTTCAGATT TGCAGGACTC GTCAGACTTG 
AAGGAACATC TGAAGGTGTA TCTTAGGATA AGACCATTTT CAGCAGTTGA GATTGAGAAT 
GGAGAATCAC AGGACTGTGT GACCATTCAG CGTCCAGACA CTGTCCTTCT CAAACCTCCA 
CAAACATCTC TGTCTGCCAG GCTCAGTGAT AAATCAGTGC CAGTTACAGC TCAGCGTTTC 
CAGTTCTCTC AGGTCTATGG TCCAGACACT ACACAGAAGG ATATATTTGA TGGCACAGTA 
AACGGACTTG TCAGAAATGT TCTAGAAGGG CAAAATTCTC TGGTCTTTAC GTATGGTGTC 
ACCAATGCTG GGAAGACCTT TACTTTTTTA GGTCCAGACA CTAATCCGGG CATCTTGCCT 
CGTTCCCTCA ACATGATCTT CAGTAGTCTG GAGGGTCGCA TCTTTACCCA GATGTGCCTG 
AAACCCCAGA GGTGCCGAGA TTTCATCAGA CTTACAAAAG AGCAACAAAA TGAGGAAGCA 
GCCAGCAAAC GCAACCTGCT CAAGCTTTTC AAAGAGTCTG ATGCTCAGAG GAGTATTTCT 
GGCCATTGCT CAAAGTCTGA TATTGAAGAT GGTTCCATGC TCTCTGACGT AAACTCTGCG 
GTTTTGGATG TGGAGATGGA CATGCACACT AGGTTTTCTA TTTGGGTCTC GTTCTGTGAG 
ATCTACAATG AGAACATCCA TGACTTGCTA GAACAGCATG CAAGCAATGC TTCACGGAAA 
ACCAACCTTC GCCTTTGTCA GGATGTCAAA GGAAACTCAT TCATTAAAGA TCTAAAATGG 
GTCCAGGTGA GCAGTGCGGA GGAAGCCCTG AAAGTGATGA AACTGGGCAA AAGAAATCAG 
AGCATCTCTA GCACTAAACT CAACCTGCTC TCCAGCAGAA GCCACAGTAT TTTCTCTGTT 
CGAATACTGA AGGTGGAGGA TGTGGGAATA CCTAGAGTGG AGTCAGTCAG CGAGCTGGCA 
CTGTGTGATC TGGCTGGATC AGAGCGTTGT GCTAAGACTC AAAATAAAGG TGAACGGCTA 
AAAGAGGCTG GCAACATCAA TACTTCTCTG CTCAGTCTTG GAAAGTGCAT TAATGCACTA 
AGGAATAACC AACAGGCTAG ACAGCATGTT CCGTTCAGAG AGAGTAAACT CACTCACTAT 
CTGCAGGGCT ACTTCACAGG CCGGGGCTCA GCCTGTATGA TTGTAAACAT TAATCAGTGC 
TCCTCCATGT ATGATGAGAC GCTGAATGTG CTCAAGTTCT CTGCCGTGGC TCAGAAGGTG 
GTGGTCCTCA CCACTAAGAA CATCCCAGTG GGAGTGAAAA GGAGTGCAAG AGAGGTGTCC 
TTCATAATCA ACAACGCTGA TTGCCGGGAA ATGAGACGCA GCTCATTAGT ACGATGGGAC 
CCAAGTCTTG AGGATGTCCA GGAAGATTCT GACTCAGAGG ACGAGTATGA AGACCAGAGC 
GAAATCGAGG ACACCTTACT AAATTCTGAA GATGAATGTA CAGAGACTGT GCAAATCAAT 
AAAAAGCTTT ATGAGGGCCA GCAGCGGTTA ATAGTGAATA CTCAGCAGCA GTTGACAGCA 
GTACAAGCCG AACTCTTAAC CCTAGAAGCA CGTGTTCGAG ATGAAGTGAC AAAAGAATTC 
TCAGAACTGA TTTCCCAGAT GCAGGAAGAT TACAGTGAAC GTATGTCCAG AGAGCGGGAG 
CTGATAGAGG AACGGTGTGA ACGGAGACTG GAGATTCTCA AAAACCTAGT GGAAAAAACG 
GCTATGGAGG ATGACCGTAT GAATTCAACT GTGGCCAAAT CAGAGGAGGA GAAACGTGTC 
TCTCTAGACA GCCTGTTTGA CTCTATGAGT GGTGACCTGG CAGGAATCAG ACATGATGCC 
CAGGCTGCCC AGTCCTGCTT GGTGACCTCT GATCCCAGAG TCTCAGCGAC ATTGCATGAC 
CTTGAGAAAA AGGTCACAGA ACTTTCAGAA CAGCTCAGCA TATCTCAGAA TCAGCTGTTG 
CTCAAAACAA AGGAGCTTGA AACCCAGAGC CGGCTGTCAG ATGAACTGGA GGAGGTGAAG 
AAGACTCTGG AGAGCCACAG GGAAAGAGAA TTTAATCTGA GTGACATGTG CAGACAGAAG 
GATGAGATGA TTGCCAAACT CCAGGCTACT TTGGACCAGC ATGTAGAAAG AAAGGCTGAG 
GATAGAGCTC AAATGGACTC TGTCCAAAAG GAGGTCATCC AGTGCTGTTG CACCATTAAA 
GAGCCCCTCA GCTCCCGGAA TGAAACCAGA AAGCGACGTC AGGAAGGAGA GGGGCTTGAA 
GGACAGCCCC CGCTCAAGAA AGGCCCCCCG GAGGACTTGA AAATGCCTGA AGATGAAGCA 
GATGATGGGC GGGAAGACTC CGCCCTTCAT GAGGTCAAAG GTCAGCTGGA GTGTTGCCTT 
GCTGAAGCCA AACAAAAAGA CAAGCAGATC ACAGACCTTG AGCAGGAATG CCACAGATTA 
CAAGTGTGCA TTCAGGATCA AAAGTCAAAT CTGGATGAAA GAGTGTCACA GGAACATATT 
CTGAAAGAAG ACGTTTGTCA GACAACATTG GAAAGCAAAC AGATGGCAAC GGCTGTGGAT 
TTTAGCAATC TGAACGTAGC AGTTGCATCC AGATGTAGCA ACACTACAGG TGCGTTTGCT 
GCTAAATCAC TAATGGCTAA GCGAGATCTT GAACTGAGAG AAAAGAACAA ACACATCTTG 
ACTCTTGAAA AGGAAATTGC GCTAATGAGA CAGAAAGCAG AACACACATC TGACTATGAA 
AATCTCAAAA AACTCAACTC TGAACTGCAA GCTGAGGTCA CGAGCTTATG TAAGGTCAAA 
GCCGAACTTG AGGAGAAGAT TACATGCCTT CAGAAGGAGA AGGAGGGGCT TGAAGATAAG 
GAGGTGGGGC TGACAAACCC TAACACTCTT GGATTTAAAA GTAAAGAGAG AGATAACCAA 
ACACACCTCA TGCTGTCTGG AAAGGAGGCT GAACTTGCAC TGAGAGAGAA TGCTCTAACT 
TTAAAGGAAA CTCAGCTTTC AGCACTACAG AAGAGTCTGA AGCAAACACA GGAGCGTCTG 
GAGGAGGAGG AGATGCAGGC GGTGCAGGAG GCGCGCAGGA AGGAGGTGGA GAGGAGGAGA 
GAGATCCTGG CTGTGGCAGA GGAGGCCATC GCTCAGAAAG ATGCAGAGCT ACAGAAGAGA 
CAAGTAGAGA TCAGCCGGTT AAAAGAGGAG ATTAAAACTA GCGAGTTTAA GATGAGCAGT 
CTGACTGTTG ATCTGCGCAG AAGGGAAGAT GATTCATCTG ATCTTCGGGA AAAACTTTGT 
GACTCAAAGA AACAAATACA GCAGGTCCAG AAAGAGATTT CGTCCATGCG TGAAGCTGAG 
AAATCTCTAC GACTGAAGCT CAGCGATCTA GAAAAAACGA AAGTGCAGCT CCAGAATGAG 
ATCGCCAACA GAGATCGCAC CATTAACCAG TTAAAAGCTG AACGATCCTC TGGTTCAAAG 
TCAGATGAGA ATCTGCTGCT CTATCAGAAA GCATGTAAAG ATCTGCAAGA GCGTGAGCGT 
GTTATTGAAG ACATGCGCTT GGCTCTGACA GAACAGGAGG AAACACAGAC ACAGCTGGAC 
CTTGAGTTGG ACAACAGAGA CGCTCGGATT GATGAGCTCA CTCAAGAGCT GGCCAGTTTG 
AGAGAAATGT TGCCTGAAGA GAGGGACAGG AGTGATTCCA GACCAATCTC TAAAGATGTT 
TTACAGGCCA GACAGCAGAT CACTCAGGCC CACGAGAGCT TACAGCTTGC CAATGAGAAG 
CAGCAGGCAG AACGCAAGAA GTGGATGGAG GAGAAGTTGA TTCTGATTGG TCAGGCTAAA 
GAAGCTGAGG AGAGGCGGAA TCAGGACATG AGACGATTTG CAGATGATCG AGAACGGCAT 
GTTCGGCAAC AGGCTGAAAT GGAGTCTCTG GCTGCACGTC TTAATGCCCG TGAGGAAGAG 
ATGGAAAGGT GGAGAAAGGA GAGAGACACG CTTGTCTCTG CTCTGGAGGT TCAGCTGAAG 
AAACTCATCA TGTCCAATGG AGAAAAAGAC CAACAGATCA AGATGCTGCA GTCCAGTAAC 
ACTGCCCAAA CACCAGAGGA GGTCAGTGAG GACAGCAGGA CCGAGGCACT GCAGGCAGAG 
CTGGCTGTAA AAGAGGCTGA GATCCAGAAA CTGAAGGAGC AGCTCAGCAT CAGTGCCACA 
AACAGCAGCA TTCGCAGAAA CTCTACTACA CAGACGATGG AAGTGGAGTT TTCTGATATG 
AAGCCTCTGA ACAGCAGTAC AATCAAGCAC AAAAGCCGAA CCAGAGGATC TGTCATCAGT 
CAGAACTCAT CCGGTAGCGG TCCACTGGTG CTAGATTCAT CAGAGATCTC CACGGAAACA 
GGCAGACGGT CTAGGTTTCC AAGGCCTGAG CTGGAGATTT CCTTCAGTCC TCTACAGCCC 
GATCGCTTCG CTCTCAAGCG TCAGGGTGAA GAATCTGCTG TCACTGTTAA AATCTCCAGA 
CCCACACGCA AGAGGAAGAG CGGCGAGATG GACAAGAGGA AAGGAGCCAG GAGCAGCGCT 
GTGAAAAAGA AAGCTTTCTC TGAGCCCAAT CCACAGGATG CGGTGGAAGG TGAAAACATC 
AGAAATACAA GGTGCAGATT GACTCCTCAC CTAACACCTC ATCGGGAAGA GTCCCCAAAT 
TCCCAGGAGT CAAACCAAAG AGGGAAAAAA GACAAAACGC TCCAGAAGAT TGGAGATTTT 
CTTCAGAGCT CTCCCACCTT TCTTGGGAGT AAAGCGAAGA GGATCATGGG ATTGATGAGT 
GGAAAATCCC CTGATGGTGG GTCGACTCCT TCTCTAAAAC TGAAATCCAA ACGTGATAAA 
CGGAAATATG ACAGACCCGA TATCTCCTCA CCAATGGACA TACCGGCTCA TCGGATTATT 
GGCCGAAATC CAGAGGACAA AGAGAGTGAA AACCTTATCA AGAAACGTCT TCGTACAAGA 
ATGGCCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif20ba ( XM_005158528.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158585.1 Danio rerio kinesin family member 20Ba (kif20ba), transcript variant X2, mRNA.

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CloneID	ODa22506
Clone ID Related Accession (Same CDS sequence)	XM_005158528.3 , XM_021473145.1 , XM_021474377.1 , XM_005158528.4
Accession Version	XM_005158528.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF20B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158528.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGT CAGGGCTAAA GGATGATCCA GCAAGACCTG CTATATTAGT TGTAGAAGAT 
CTAAGGAGAA ATTTGTCCAC AGAGTTTGAT TGTTCAGATT TGCAGGACTC GTCAGACTTG 
AAGGAACATC TGAAGGTGTA TCTTAGGATA AGACCATTTT CAGCAGTTGA GATTGAGAAT 
GGAGAATCAC AGGACTGTGT GACCATTCAG CGTCCAGACA CTGTCCTTCT CAAACCTCCA 
CAAACATCTC TGTCTGCCAG GCTCAGTGAT AAATCAGTGC CAGTTACAGC TCAGCGTTTC 
CAGTTCTCTC AGGTCTATGG TCCAGACACT ACACAGAAGG ATATATTTGA TGGCACAGTA 
AACGGACTTG TCAGAAATGT TCTAGAAGGG CAAAATTCTC TGGTCTTTAC GTATGGTGTC 
ACCAATGCTG GGAAGACCTT TACTTTTTTA GGTCCAGACA CTAATCCGGG CATCTTGCCT 
CGTTCCCTCA ACATGATCTT CAGTAGTCTG GAGGGTCGCA TCTTTACCCA GATGTGCCTG 
AAACCCCAGA GGTGCCGAGA TTTCATCAGA CTTACAAAAG AGCAACAAAA TGAGGAAGCA 
GCCAGCAAAC GCAACCTGCT CAAGCTTTTC AAAGAGTCTG ATGCTCAGAG GAGTATTTCT 
GGCCATTGCT CAAAGTCTGA TATTGAAGAT GGTTCCATGC TCTCTGACGT AAACTCTGCG 
GTTTTGGATG TGGAGATGGA CATGCACACT AGGTTTTCTA TTTGGGTCTC GTTCTGTGAG 
ATCTACAATG AGAACATCCA TGACTTGCTA GAACAGCATG CAAGCAATGC TTCACGGAAA 
ACCAACCTTC GCCTTTGTCA GGATGTCAAA GGAAACTCAT TCATTAAAGA TCTAAAATGG 
GTCCAGGTGA GCAGTGCGGA GGAAGCCCTG AAAGTGATGA AACTGGGCAA AAGAAATCAG 
AGCATCTCTA GCACTAAACT CAACCTGCTC TCCAGCAGAA GCCACAGTAT TTTCTCTGTT 
CGAATACTGA AGGTGGAGGA TGTGGGAATA CCTAGAGTGG AGTCAGTCAG CGAGCTGGCA 
CTGTGTGATC TGGCTGGATC AGAGCGTTGT GCTAAGACTC AAAATAAAGG TGAACGGCTA 
AAAGAGGCTG GCAACATCAA TACTTCTCTG CTCAGTCTTG GAAAGTGCAT TAATGCACTA 
AGGAATAACC AACAGGCTAG ACAGCATGTT CCGTTCAGAG AGAGTAAACT CACTCACTAT 
CTGCAGGGCT ACTTCACAGG CCGGGGCTCA GCCTGTATGA TTGTAAACAT TAATCAGTGC 
TCCTCCATGT ATGATGAGAC GCTGAATGTG CTCAAGTTCT CTGCCGTGGC TCAGAAGGTG 
GTGGTCCTCA CCACTAAGAA CATCCCAGTG GGAGTGAAAA GGAGTGCAAG AGAGGTGTCC 
TTCATAATCA ACAACGCTGA TTGCCGGGAA ATGAGACGCA GCTCATTAGT ACGATGGGAC 
CCAAGTCTTG AGGATGTCCA GGAAGATTCT GACTCAGAGG ACGAGTATGA AGACCAGAGC 
GAAATCGAGG ACACCTTACT AAATTCTGAA GATGAATGTA CAGAGACTGT GCAAATCAAT 
AAAAAGCTTT ATGAGGGCCA GCAGCGGTTA ATAGTGAATA CTCAGCAGCA GTTGACAGCA 
GTACAAGCCG AACTCTTAAC CCTAGAAGCA CGTGTTCGAG ATGAAGTGAC AAAAGAATTC 
TCAGAACTGA TTTCCCAGAT GCAGGAAGAT TACAGTGAAC GTATGTCCAG AGAGCGGGAG 
CTGATAGAGG AACGGTGTGA ACGGAGACTG GAGATTCTCA AAAACCTAGT GGAAAAAACG 
GCTATGGAGG ATGACCGTAT GAATTCAACT GTGGCCAAAT CAGAGGAGGA GAAACGTGTC 
TCTCTAGACA GCCTGTTTGA CTCTATGAGT GGTGACCTGG CAGGAATCAG ACATGATGCC 
CAGGCTGCCC AGTCCTGCTT GGTGACCTCT GATCCCAGAG TCTCAGCGAC ATTGCATGAC 
CTTGAGAAAA AGGTCACAGA ACTTTCAGAA CAGCTCAGCA TATCTCAGAA TCAGCTGTTG 
CTCAAAACAA AGGAGCTTGA AACCCAGAGC CGGCTGTCAG ATGAACTGGA GGAGGTGAAG 
AAGACTCTGG AGAGCCACAG GGAAAGAGAA TTTAATCTGA GTGACATGTG CAGACAGAAG 
GATGAGATGA TTGCCAAACT CCAGGCTACT TTGGACCAGC ATGTAGAAAG AAAGGCTGAG 
GATAGAGCTC AAATGGACTC TGTCCAAAAG GAGGTCATCC AGTGCTGTTG CACCATTAAA 
GAGCCCCTCA GCTCCCGGAA TGAAACCAGA AAGCGACGTC AGGAAGGAGA GGGGCTTGAA 
GGACAGCCCC CGCTCAAGAA AGGCCCCCCG GAGGACTTGA AAATGCCTGA AGATGAAGCA 
GATGATGGGC GGGAAGACTC CGCCCTTCAT GAGGTCAAAG GTCAGCTGGA GTGTTGCCTT 
GCTGAAGCCA AACAAAAAGA CAAGCAGATC ACAGACCTTG AGCAGGAATG CCACAGATTA 
CAAGTGTGCA TTCAGGATCA AAAGTCAAAT CTGGATGAAA GAGTGTCACA GGAACATATT 
CTGAAAGAAG ACGTTTGTCA GACAACATTG GAAAGCAAAC AGATGGCAAC GGCTGTGGAT 
TTTAGCAATC TGAACGTAGC AGTTGCATCC AGATGTAGCA ACACTACAGG TGCGTTTGCT 
GCTAAATCAC TAATGGCTAA GCGAGATCTT GAACTGAGAG AAAAGAACAA ACACATCTTG 
ACTCTTGAAA AGGAAATTGC GCTAATGAGA CAGAAAGCAG AACACACATC TGACTATGAA 
AATCTCAAAA AACTCAACTC TGAACTGCAA GCTGAGGTCA CGAGCTTATG TAAGGTCAAA 
GCCGAACTTG AGGAGAAGAT TACATGCCTT CAGAAGGAGA AGGAGGGGCT TGAAGATAAG 
GAGGTGGGGC TGACAAACCC TAACACTCTT GGATTTAAAA GTAAAGAGAG AGATAACCAA 
ACACACCTCA TGCTGTCTGG AAAGGAGGCT GAACTTGCAC TGAGAGAGAA TGCTCTAACT 
TTAAAGGAAA CTCAGCTTTC AGCACTACAG AAGAGTCTGA AGCAAACACA GGAGCGTCTG 
GAGGAGGAGG AGATGCAGGC GGTGCAGGAG GCGCGCAGGA AGGAGGTGGA GAGGAGGAGA 
GAGATCCTGG CTGTGGCAGA GGAGGCCATC GCTCAGAAAG ATGCAGAGCT ACAGAAGAGA 
CAAGTAGAGA TCAGCCGGTT AAAAGAGGAG ATTAAAACTA GCGAGTTTAA GATGAGCAGT 
CTGACTGTTG ATCTGCGCAG AAGGGAAGAT GATTCATCTG ATCTTCGGGA AAAACTTTGT 
GACTCAAAGA AACAAATACA GCAGGTCCAG AAAGAGATTT CGTCCATGCG TGAAGCTGAG 
AAATCTCTAC GACTGAAGCT CAGCGATCTA GAAAAAACGA AAGTGCAGCT CCAGAATGAG 
ATCGCCAACA GAGATCGCAC CATTAACCAG TTAAAAGCTG AACGATCCTC TGGTTCAAAG 
TCAGATGAGA ATCTGCTGCT CTATCAGAAA GCATGTAAAG ATCTGCAAGA GCGTGAGCGT 
GTTATTGAAG ACATGCGCTT GGCTCTGACA GAACAGGAGG AAACACAGAC ACAGCTGGAC 
CTTGAGTTGG ACAACAGAGA CGCTCGGATT GATGAGCTCA CTCAAGAGCT GGCCAGTTTG 
AGAGAAATGT TGCCTGAAGA GAGGGACAGG AGTGATTCCA GACCAATCTC TAAAGATGTT 
TTACAGGCCA GACAGCAGAT CACTCAGGCC CACGAGAGCT TACAGCTTGC CAATGAGAAG 
CAGCAGGCAG AACGCAAGAA GTGGATGGAG GAGAAGTTGA TTCTGATTGG TCAGGCTAAA 
GAAGCTGAGG AGAGGCGGAA TCAGGACATG AGACGATTTG CAGATGATCG AGAACGGCAT 
GTTCGGCAAC AGGCTGAAAT GGAGTCTCTG GCTGCACGTC TTAATGCCCG TGAGGAAGAG 
ATGGAAAGGT GGAGAAAGGA GAGAGACACG CTTGTCTCTG CTCTGGAGGT TCAGCTGAAG 
AAACTCATCA TGTCCAATGG AGAAAAAGAC CAACAGATCA AGATGCTGCA GTCCAGTAAC 
ACTGCCCAAA CACCAGAGGA GGTCAGTGAG GACAGCAGGA CCGAGGCACT GCAGGCAGAG 
CTGGCTGTAA AAGAGGCTGA GATCCAGAAA CTGAAGGAGC AGCTCAGCAT CAGTGCCACA 
AACAGCAGCA TTCGCAGAAA CTCTACTACA CAGACGATGG AAGTGGAGTT TTCTGATATG 
AAGCCTCTGA ACAGCAGTAC AATCAAGCAC AAAAGCCGAA CCAGAGGATC TGTCATCAGT 
CAGAACTCAT CCGGTAGCGG TCCACTGGTG CTAGATTCAT CAGAGATCTC CACGGAAACA 
GGCAGACGGT CTAGGTTTCC AAGGCCTGAG CTGGAGATTT CCTTCAGTCC TCTACAGCCC 
GATCGCTTCG CTCTCAAGCG TCAGGGTGAA GAATCTGCTG TCACTGTTAA AATCTCCAGA 
CCCACACGCA AGAGGAAGAG CGGCGAGATG GACAAGGATG CGGTGGAAGG TGAAAACATC 
AGAAATACAA GGTGCAGATT GACTCCTCAC CTAACACCTC ATCGGGAAGA GTCCCCAAAT 
TCCCAGGAGT CAAACCAAAG AGGGAAAAAA GACAAAACGC TCCAGAAGAT TGGAGATTTT 
CTTCAGAGCT CTCCCACCTT TCTTGGGAGT AAAGCGAAGA GGATCATGGG ATTGATGAGT 
GGAAAATCCC CTGATGGTGG GTCGACTCCT TCTCTAAAAC TGAAATCCAA ACGTGATAAA 
CGGAAATATG ACAGACCCGA TATCTCCTCA CCAATGGACA TACCGGCTCA TCGGATTATT 
GGCCGAAATC CAGAGGACAA AGAGAGTGAA AACCTTATCA AGAAACGTCT TCGTACAAGA 
ATGGCCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158585.1
CDS	154..5205
Translation	MMESGLKDDPARPAILVVEDLRRNLSTEFDCSDLQDSSDLKEHLKVYLRIRPFSAVEIENGESQDCVTIQRPDTVLLKPPQTSLSARLSDKSVPVTAQRFQFSQVYGPDTTQKDIFDGTVNGLVRNVLEGQNSLVFTYGVTNAGKTFTFLGPDTNPGILPRSLNMIFSSLEGRIFTQMCLKPQRCRDFIRLTKEQQNEEAASKRNLLKLFKESDAQRSISGHCSKSDIEDGSMLSDVNSAVLDVEMDMHTRFSIWVSFCEIYNENIHDLLEQHASNASRKTNLRLCQDVKGNSFIKDLKWVQVSSAEEALKVMKLGKRNQSISSTKLNLLSSRSHSIFSVRILKVEDVGIPRVESVSELALCDLAGSERCAKTQNKGERLKEAGNINTSLLSLGKCINALRNNQQARQHVPFRESKLTHYLQGYFTGRGSACMIVNINQCSSMYDETLNVLKFSAVAQKVVVLTTKNIPVGVKRSAREVSFIINNADCREMRRSSLVRWDPSLEDVQEDSDSEDEYEDQSEIEDTLLNSEDECTETVQINKKLYEGQQRLIVNTQQQLTAVQAELLTLEARVRDEVTKEFSELISQMQEDYSERMSRERELIEERCERRLEILKNLVEKTAMEDDRMNSTVAKSEEEKRVSLDSLFDSMSGDLAGIRHDAQAAQSCLVTSDPRVSATLHDLEKKVTELSEQLSISQNQLLLKTKELETQSRLSDELEEVKKTLESHREREFNLSDMCRQKDEMIAKLQATLDQHVERKAEDRAQMDSVQKEVIQCCCTIKEPLSSRNETRKRRQEGEGLEGQPPLKKGPPEDLKMPEDEADDGREDSALHEVKGQLECCLAEAKQKDKQITDLEQECHRLQVCIQDQKSNLDERVSQEHILKEDVCQTTLESKQMATAVDFSNLNVAVASRCSNTTGAFAAKSLMAKRDLELREKNKHILTLEKEIALMRQKAEHTSDYENLKKLNSELQAEVTSLCKVKAELEEKITCLQKEKEGLEDKEVGLTNPNTLGFKSKERDNQTHLMLSGKEAELALRENALTLKETQLSALQKSLKQTQERLEEEEMQAVQEARRKEVERRREILAVAEEAIAQKDAELQKRQVEISRLKEEIKTSEFKMSSLTVDLRRREDDSSDLREKLCDSKKQIQQVQKEISSMREAEKSLRLKLSDLEKTKVQLQNEIANRDRTINQLKAERSSGSKSDENLLLYQKACKDLQERERVIEDMRLALTEQEETQTQLDLELDNRDARIDELTQELASLREMLPEERDRSDSRPISKDVLQARQQITQAHESLQLANEKQQAERKKWMEEKLILIGQAKEAEERRNQDMRRFADDRERHVRQQAEMESLAARLNAREEEMERWRKERDTLVSALEVQLKKLIMSNGEKDQQIKMLQSSNTAQTPEEVSEDSRTEALQAELAVKEAEIQKLKEQLSISATNSSIRRNSTTQTMEVEFSDMKPLNSSTIKHKSRTRGSVISQNSSGSGPLVLDSSEISTETGRRSRFPRPELEISFSPLQPDRFALKRQGEESAVTVKISRPTRKRKSGEMDKDAVEGENIRNTRCRLTPHLTPHREESPNSQESNQRGKKDKTLQKIGDFLQSSPTFLGSKAKRIMGLMSGKSPDGGSTPSLKLKSKRDKRKYDRPDISSPMDIPAHRIIGRNPEDKESENLIKKRLRTRMAK

Target ORF information:

RefSeq Version	XM_005158528.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 20Ba (kif20ba), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158528.3

ORF Insert Sequence:

ATGATGGAGT CAGGGCTAAA GGATGATCCA GCAAGACCTG CTATATTAGT TGTAGAAGAT 
CTAAGGAGAA ATTTGTCCAC AGAGTTTGAT TGTTCAGATT TGCAGGACTC GTCAGACTTG 
AAGGAACATC TGAAGGTGTA TCTTAGGATA AGACCATTTT CAGCAGTTGA GATTGAGAAT 
GGAGAATCAC AGGACTGTGT GACCATTCAG CGTCCAGACA CTGTCCTTCT CAAACCTCCA 
CAAACATCTC TGTCTGCCAG GCTCAGTGAT AAATCAGTGC CAGTTACAGC TCAGCGTTTC 
CAGTTCTCTC AGGTCTATGG TCCAGACACT ACACAGAAGG ATATATTTGA TGGCACAGTA 
AACGGACTTG TCAGAAATGT TCTAGAAGGG CAAAATTCTC TGGTCTTTAC GTATGGTGTC 
ACCAATGCTG GGAAGACCTT TACTTTTTTA GGTCCAGACA CTAATCCGGG CATCTTGCCT 
CGTTCCCTCA ACATGATCTT CAGTAGTCTG GAGGGTCGCA TCTTTACCCA GATGTGCCTG 
AAACCCCAGA GGTGCCGAGA TTTCATCAGA CTTACAAAAG AGCAACAAAA TGAGGAAGCA 
GCCAGCAAAC GCAACCTGCT CAAGCTTTTC AAAGAGTCTG ATGCTCAGAG GAGTATTTCT 
GGCCATTGCT CAAAGTCTGA TATTGAAGAT GGTTCCATGC TCTCTGACGT AAACTCTGCG 
GTTTTGGATG TGGAGATGGA CATGCACACT AGGTTTTCTA TTTGGGTCTC GTTCTGTGAG 
ATCTACAATG AGAACATCCA TGACTTGCTA GAACAGCATG CAAGCAATGC TTCACGGAAA 
ACCAACCTTC GCCTTTGTCA GGATGTCAAA GGAAACTCAT TCATTAAAGA TCTAAAATGG 
GTCCAGGTGA GCAGTGCGGA GGAAGCCCTG AAAGTGATGA AACTGGGCAA AAGAAATCAG 
AGCATCTCTA GCACTAAACT CAACCTGCTC TCCAGCAGAA GCCACAGTAT TTTCTCTGTT 
CGAATACTGA AGGTGGAGGA TGTGGGAATA CCTAGAGTGG AGTCAGTCAG CGAGCTGGCA 
CTGTGTGATC TGGCTGGATC AGAGCGTTGT GCTAAGACTC AAAATAAAGG TGAACGGCTA 
AAAGAGGCTG GCAACATCAA TACTTCTCTG CTCAGTCTTG GAAAGTGCAT TAATGCACTA 
AGGAATAACC AACAGGCTAG ACAGCATGTT CCGTTCAGAG AGAGTAAACT CACTCACTAT 
CTGCAGGGCT ACTTCACAGG CCGGGGCTCA GCCTGTATGA TTGTAAACAT TAATCAGTGC 
TCCTCCATGT ATGATGAGAC GCTGAATGTG CTCAAGTTCT CTGCCGTGGC TCAGAAGGTG 
GTGGTCCTCA CCACTAAGAA CATCCCAGTG GGAGTGAAAA GGAGTGCAAG AGAGGTGTCC 
TTCATAATCA ACAACGCTGA TTGCCGGGAA ATGAGACGCA GCTCATTAGT ACGATGGGAC 
CCAAGTCTTG AGGATGTCCA GGAAGATTCT GACTCAGAGG ACGAGTATGA AGACCAGAGC 
GAAATCGAGG ACACCTTACT AAATTCTGAA GATGAATGTA CAGAGACTGT GCAAATCAAT 
AAAAAGCTTT ATGAGGGCCA GCAGCGGTTA ATAGTGAATA CTCAGCAGCA GTTGACAGCA 
GTACAAGCCG AACTCTTAAC CCTAGAAGCA CGTGTTCGAG ATGAAGTGAC AAAAGAATTC 
TCAGAACTGA TTTCCCAGAT GCAGGAAGAT TACAGTGAAC GTATGTCCAG AGAGCGGGAG 
CTGATAGAGG AACGGTGTGA ACGGAGACTG GAGATTCTCA AAAACCTAGT GGAAAAAACG 
GCTATGGAGG ATGACCGTAT GAATTCAACT GTGGCCAAAT CAGAGGAGGA GAAACGTGTC 
TCTCTAGACA GCCTGTTTGA CTCTATGAGT GGTGACCTGG CAGGAATCAG ACATGATGCC 
CAGGCTGCCC AGTCCTGCTT GGTGACCTCT GATCCCAGAG TCTCAGCGAC ATTGCATGAC 
CTTGAGAAAA AGGTCACAGA ACTTTCAGAA CAGCTCAGCA TATCTCAGAA TCAGCTGTTG 
CTCAAAACAA AGGAGCTTGA AACCCAGAGC CGGCTGTCAG ATGAACTGGA GGAGGTGAAG 
AAGACTCTGG AGAGCCACAG GGAAAGAGAA TTTAATCTGA GTGACATGTG CAGACAGAAG 
GATGAGATGA TTGCCAAACT CCAGGCTACT TTGGACCAGC ATGTAGAAAG AAAGGCTGAG 
GATAGAGCTC AAATGGACTC TGTCCAAAAG GAGGTCATCC AGTGCTGTTG CACCATTAAA 
GAGCCCCTCA GCTCCCGGAA TGAAACCAGA AAGCGACGTC AGGAAGGAGA GGGGCTTGAA 
GGACAGCCCC CGCTCAAGAA AGGCCCCCCG GAGGACTTGA AAATGCCTGA AGATGAAGCA 
GATGATGGGC GGGAAGACTC CGCCCTTCAT GAGGTCAAAG GTCAGCTGGA GTGTTGCCTT 
GCTGAAGCCA AACAAAAAGA CAAGCAGATC ACAGACCTTG AGCAGGAATG CCACAGATTA 
CAAGTGTGCA TTCAGGATCA AAAGTCAAAT CTGGATGAAA GAGTGTCACA GGAACATATT 
CTGAAAGAAG ACGTTTGTCA GACAACATTG GAAAGCAAAC AGATGGCAAC GGCTGTGGAT 
TTTAGCAATC TGAACGTAGC AGTTGCATCC AGATGTAGCA ACACTACAGG TGCGTTTGCT 
GCTAAATCAC TAATGGCTAA GCGAGATCTT GAACTGAGAG AAAAGAACAA ACACATCTTG 
ACTCTTGAAA AGGAAATTGC GCTAATGAGA CAGAAAGCAG AACACACATC TGACTATGAA 
AATCTCAAAA AACTCAACTC TGAACTGCAA GCTGAGGTCA CGAGCTTATG TAAGGTCAAA 
GCCGAACTTG AGGAGAAGAT TACATGCCTT CAGAAGGAGA AGGAGGGGCT TGAAGATAAG 
GAGGTGGGGC TGACAAACCC TAACACTCTT GGATTTAAAA GTAAAGAGAG AGATAACCAA 
ACACACCTCA TGCTGTCTGG AAAGGAGGCT GAACTTGCAC TGAGAGAGAA TGCTCTAACT 
TTAAAGGAAA CTCAGCTTTC AGCACTACAG AAGAGTCTGA AGCAAACACA GGAGCGTCTG 
GAGGAGGAGG AGATGCAGGC GGTGCAGGAG GCGCGCAGGA AGGAGGTGGA GAGGAGGAGA 
GAGATCCTGG CTGTGGCAGA GGAGGCCATC GCTCAGAAAG ATGCAGAGCT ACAGAAGAGA 
CAAGTAGAGA TCAGCCGGTT AAAAGAGGAG ATTAAAACTA GCGAGTTTAA GATGAGCAGT 
CTGACTGTTG ATCTGCGCAG AAGGGAAGAT GATTCATCTG ATCTTCGGGA AAAACTTTGT 
GACTCAAAGA AACAAATACA GCAGGTCCAG AAAGAGATTT CGTCCATGCG TGAAGCTGAG 
AAATCTCTAC GACTGAAGCT CAGCGATCTA GAAAAAACGA AAGTGCAGCT CCAGAATGAG 
ATCGCCAACA GAGATCGCAC CATTAACCAG TTAAAAGCTG AACGATCCTC TGGTTCAAAG 
TCAGATGAGA ATCTGCTGCT CTATCAGAAA GCATGTAAAG ATCTGCAAGA GCGTGAGCGT 
GTTATTGAAG ACATGCGCTT GGCTCTGACA GAACAGGAGG AAACACAGAC ACAGCTGGAC 
CTTGAGTTGG ACAACAGAGA CGCTCGGATT GATGAGCTCA CTCAAGAGCT GGCCAGTTTG 
AGAGAAATGT TGCCTGAAGA GAGGGACAGG AGTGATTCCA GACCAATCTC TAAAGATGTT 
TTACAGGCCA GACAGCAGAT CACTCAGGCC CACGAGAGCT TACAGCTTGC CAATGAGAAG 
CAGCAGGCAG AACGCAAGAA GTGGATGGAG GAGAAGTTGA TTCTGATTGG TCAGGCTAAA 
GAAGCTGAGG AGAGGCGGAA TCAGGACATG AGACGATTTG CAGATGATCG AGAACGGCAT 
GTTCGGCAAC AGGCTGAAAT GGAGTCTCTG GCTGCACGTC TTAATGCCCG TGAGGAAGAG 
ATGGAAAGGT GGAGAAAGGA GAGAGACACG CTTGTCTCTG CTCTGGAGGT TCAGCTGAAG 
AAACTCATCA TGTCCAATGG AGAAAAAGAC CAACAGATCA AGATGCTGCA GTCCAGTAAC 
ACTGCCCAAA CACCAGAGGA GGTCAGTGAG GACAGCAGGA CCGAGGCACT GCAGGCAGAG 
CTGGCTGTAA AAGAGGCTGA GATCCAGAAA CTGAAGGAGC AGCTCAGCAT CAGTGCCACA 
AACAGCAGCA TTCGCAGAAA CTCTACTACA CAGACGATGG AAGTGGAGTT TTCTGATATG 
AAGCCTCTGA ACAGCAGTAC AATCAAGCAC AAAAGCCGAA CCAGAGGATC TGTCATCAGT 
CAGAACTCAT CCGGTAGCGG TCCACTGGTG CTAGATTCAT CAGAGATCTC CACGGAAACA 
GGCAGACGGT CTAGGTTTCC AAGGCCTGAG CTGGAGATTT CCTTCAGTCC TCTACAGCCC 
GATCGCTTCG CTCTCAAGCG TCAGGGTGAA GAATCTGCTG TCACTGTTAA AATCTCCAGA 
CCCACACGCA AGAGGAAGAG CGGCGAGATG GACAAGGATG CGGTGGAAGG TGAAAACATC 
AGAAATACAA GGTGCAGATT GACTCCTCAC CTAACACCTC ATCGGGAAGA GTCCCCAAAT 
TCCCAGGAGT CAAACCAAAG AGGGAAAAAA GACAAAACGC TCCAGAAGAT TGGAGATTTT 
CTTCAGAGCT CTCCCACCTT TCTTGGGAGT AAAGCGAAGA GGATCATGGG ATTGATGAGT 
GGAAAATCCC CTGATGGTGG GTCGACTCCT TCTCTAAAAC TGAAATCCAA ACGTGATAAA 
CGGAAATATG ACAGACCCGA TATCTCCTCA CCAATGGACA TACCGGCTCA TCGGATTATT 
GGCCGAAATC CAGAGGACAA AGAGAGTGAA AACCTTATCA AGAAACGTCT TCGTACAAGA 
ATGGCCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif20ba ( XM_021473145.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328820.1 Danio rerio kinesin family member 20Ba (kif20ba), transcript variant X2, mRNA.

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CloneID	ODa22506
Clone ID Related Accession (Same CDS sequence)	XM_005158528.3 , XM_021473145.1 , XM_021474377.1 , XM_005158528.4
Accession Version	XM_021473145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF20B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394988.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGT CAGGGCTAAA GGATGATCCA GCAAGACCTG CTATATTAGT TGTAGAAGAT 
CTAAGGAGAA ATTTGTCCAC AGAGTTTGAT TGTTCAGATT TGCAGGACTC GTCAGACTTG 
AAGGAACATC TGAAGGTGTA TCTTAGGATA AGACCATTTT CAGCAGTTGA GATTGAGAAT 
GGAGAATCAC AGGACTGTGT GACCATTCAG CGTCCAGACA CTGTCCTTCT CAAACCTCCA 
CAAACATCTC TGTCTGCCAG GCTCAGTGAT AAATCAGTGC CAGTTACAGC TCAGCGTTTC 
CAGTTCTCTC AGGTCTATGG TCCAGACACT ACACAGAAGG ATATATTTGA TGGCACAGTA 
AACGGACTTG TCAGAAATGT TCTAGAAGGG CAAAATTCTC TGGTCTTTAC GTATGGTGTC 
ACCAATGCTG GGAAGACCTT TACTTTTTTA GGTCCAGACA CTAATCCGGG CATCTTGCCT 
CGTTCCCTCA ACATGATCTT CAGTAGTCTG GAGGGTCGCA TCTTTACCCA GATGTGCCTG 
AAACCCCAGA GGTGCCGAGA TTTCATCAGA CTTACAAAAG AGCAACAAAA TGAGGAAGCA 
GCCAGCAAAC GCAACCTGCT CAAGCTTTTC AAAGAGTCTG ATGCTCAGAG GAGTATTTCT 
GGCCATTGCT CAAAGTCTGA TATTGAAGAT GGTTCCATGC TCTCTGACGT AAACTCTGCG 
GTTTTGGATG TGGAGATGGA CATGCACACT AGGTTTTCTA TTTGGGTCTC GTTCTGTGAG 
ATCTACAATG AGAACATCCA TGACTTGCTA GAACAGCATG CAAGCAATGC TTCACGGAAA 
ACCAACCTTC GCCTTTGTCA GGATGTCAAA GGAAACTCAT TCATTAAAGA TCTAAAATGG 
GTCCAGGTGA GCAGTGCGGA GGAAGCCCTG AAAGTGATGA AACTGGGCAA AAGAAATCAG 
AGCATCTCTA GCACTAAACT CAACCTGCTC TCCAGCAGAA GCCACAGTAT TTTCTCTGTT 
CGAATACTGA AGGTGGAGGA TGTGGGAATA CCTAGAGTGG AGTCAGTCAG CGAGCTGGCA 
CTGTGTGATC TGGCTGGATC AGAGCGTTGT GCTAAGACTC AAAATAAAGG TGAACGGCTA 
AAAGAGGCTG GCAACATCAA TACTTCTCTG CTCAGTCTTG GAAAGTGCAT TAATGCACTA 
AGGAATAACC AACAGGCTAG ACAGCATGTT CCGTTCAGAG AGAGTAAACT CACTCACTAT 
CTGCAGGGCT ACTTCACAGG CCGGGGCTCA GCCTGTATGA TTGTAAACAT TAATCAGTGC 
TCCTCCATGT ATGATGAGAC GCTGAATGTG CTCAAGTTCT CTGCCGTGGC TCAGAAGGTG 
GTGGTCCTCA CCACTAAGAA CATCCCAGTG GGAGTGAAAA GGAGTGCAAG AGAGGTGTCC 
TTCATAATCA ACAACGCTGA TTGCCGGGAA ATGAGACGCA GCTCATTAGT ACGATGGGAC 
CCAAGTCTTG AGGATGTCCA GGAAGATTCT GACTCAGAGG ACGAGTATGA AGACCAGAGC 
GAAATCGAGG ACACCTTACT AAATTCTGAA GATGAATGTA CAGAGACTGT GCAAATCAAT 
AAAAAGCTTT ATGAGGGCCA GCAGCGGTTA ATAGTGAATA CTCAGCAGCA GTTGACAGCA 
GTACAAGCCG AACTCTTAAC CCTAGAAGCA CGTGTTCGAG ATGAAGTGAC AAAAGAATTC 
TCAGAACTGA TTTCCCAGAT GCAGGAAGAT TACAGTGAAC GTATGTCCAG AGAGCGGGAG 
CTGATAGAGG AACGGTGTGA ACGGAGACTG GAGATTCTCA AAAACCTAGT GGAAAAAACG 
GCTATGGAGG ATGACCGTAT GAATTCAACT GTGGCCAAAT CAGAGGAGGA GAAACGTGTC 
TCTCTAGACA GCCTGTTTGA CTCTATGAGT GGTGACCTGG CAGGAATCAG ACATGATGCC 
CAGGCTGCCC AGTCCTGCTT GGTGACCTCT GATCCCAGAG TCTCAGCGAC ATTGCATGAC 
CTTGAGAAAA AGGTCACAGA ACTTTCAGAA CAGCTCAGCA TATCTCAGAA TCAGCTGTTG 
CTCAAAACAA AGGAGCTTGA AACCCAGAGC CGGCTGTCAG ATGAACTGGA GGAGGTGAAG 
AAGACTCTGG AGAGCCACAG GGAAAGAGAA TTTAATCTGA GTGACATGTG CAGACAGAAG 
GATGAGATGA TTGCCAAACT CCAGGCTACT TTGGACCAGC ATGTAGAAAG AAAGGCTGAG 
GATAGAGCTC AAATGGACTC TGTCCAAAAG GAGGTCATCC AGTGCTGTTG CACCATTAAA 
GAGCCCCTCA GCTCCCGGAA TGAAACCAGA AAGCGACGTC AGGAAGGAGA GGGGCTTGAA 
GGACAGCCCC CGCTCAAGAA AGGCCCCCCG GAGGACTTGA AAATGCCTGA AGATGAAGCA 
GATGATGGGC GGGAAGACTC CGCCCTTCAT GAGGTCAAAG GTCAGCTGGA GTGTTGCCTT 
GCTGAAGCCA AACAAAAAGA CAAGCAGATC ACAGACCTTG AGCAGGAATG CCACAGATTA 
CAAGTGTGCA TTCAGGATCA AAAGTCAAAT CTGGATGAAA GAGTGTCACA GGAACATATT 
CTGAAAGAAG ACGTTTGTCA GACAACATTG GAAAGCAAAC AGATGGCAAC GGCTGTGGAT 
TTTAGCAATC TGAACGTAGC AGTTGCATCC AGATGTAGCA ACACTACAGG TGCGTTTGCT 
GCTAAATCAC TAATGGCTAA GCGAGATCTT GAACTGAGAG AAAAGAACAA ACACATCTTG 
ACTCTTGAAA AGGAAATTGC GCTAATGAGA CAGAAAGCAG AACACACATC TGACTATGAA 
AATCTCAAAA AACTCAACTC TGAACTGCAA GCTGAGGTCA CGAGCTTATG TAAGGTCAAA 
GCCGAACTTG AGGAGAAGAT TACATGCCTT CAGAAGGAGA AGGAGGGGCT TGAAGATAAG 
GAGGTGGGGC TGACAAACCC TAACACTCTT GGATTTAAAA GTAAAGAGAG AGATAACCAA 
ACACACCTCA TGCTGTCTGG AAAGGAGGCT GAACTTGCAC TGAGAGAGAA TGCTCTAACT 
TTAAAGGAAA CTCAGCTTTC AGCACTACAG AAGAGTCTGA AGCAAACACA GGAGCGTCTG 
GAGGAGGAGG AGATGCAGGC GGTGCAGGAG GCGCGCAGGA AGGAGGTGGA GAGGAGGAGA 
GAGATCCTGG CTGTGGCAGA GGAGGCCATC GCTCAGAAAG ATGCAGAGCT ACAGAAGAGA 
CAAGTAGAGA TCAGCCGGTT AAAAGAGGAG ATTAAAACTA GCGAGTTTAA GATGAGCAGT 
CTGACTGTTG ATCTGCGCAG AAGGGAAGAT GATTCATCTG ATCTTCGGGA AAAACTTTGT 
GACTCAAAGA AACAAATACA GCAGGTCCAG AAAGAGATTT CGTCCATGCG TGAAGCTGAG 
AAATCTCTAC GACTGAAGCT CAGCGATCTA GAAAAAACGA AAGTGCAGCT CCAGAATGAG 
ATCGCCAACA GAGATCGCAC CATTAACCAG TTAAAAGCTG AACGATCCTC TGGTTCAAAG 
TCAGATGAGA ATCTGCTGCT CTATCAGAAA GCATGTAAAG ATCTGCAAGA GCGTGAGCGT 
GTTATTGAAG ACATGCGCTT GGCTCTGACA GAACAGGAGG AAACACAGAC ACAGCTGGAC 
CTTGAGTTGG ACAACAGAGA CGCTCGGATT GATGAGCTCA CTCAAGAGCT GGCCAGTTTG 
AGAGAAATGT TGCCTGAAGA GAGGGACAGG AGTGATTCCA GACCAATCTC TAAAGATGTT 
TTACAGGCCA GACAGCAGAT CACTCAGGCC CACGAGAGCT TACAGCTTGC CAATGAGAAG 
CAGCAGGCAG AACGCAAGAA GTGGATGGAG GAGAAGTTGA TTCTGATTGG TCAGGCTAAA 
GAAGCTGAGG AGAGGCGGAA TCAGGACATG AGACGATTTG CAGATGATCG AGAACGGCAT 
GTTCGGCAAC AGGCTGAAAT GGAGTCTCTG GCTGCACGTC TTAATGCCCG TGAGGAAGAG 
ATGGAAAGGT GGAGAAAGGA GAGAGACACG CTTGTCTCTG CTCTGGAGGT TCAGCTGAAG 
AAACTCATCA TGTCCAATGG AGAAAAAGAC CAACAGATCA AGATGCTGCA GTCCAGTAAC 
ACTGCCCAAA CACCAGAGGA GGTCAGTGAG GACAGCAGGA CCGAGGCACT GCAGGCAGAG 
CTGGCTGTAA AAGAGGCTGA GATCCAGAAA CTGAAGGAGC AGCTCAGCAT CAGTGCCACA 
AACAGCAGCA TTCGCAGAAA CTCTACTACA CAGACGATGG AAGTGGAGTT TTCTGATATG 
AAGCCTCTGA ACAGCAGTAC AATCAAGCAC AAAAGCCGAA CCAGAGGATC TGTCATCAGT 
CAGAACTCAT CCGGTAGCGG TCCACTGGTG CTAGATTCAT CAGAGATCTC CACGGAAACA 
GGCAGACGGT CTAGGTTTCC AAGGCCTGAG CTGGAGATTT CCTTCAGTCC TCTACAGCCC 
GATCGCTTCG CTCTCAAGCG TCAGGGTGAA GAATCTGCTG TCACTGTTAA AATCTCCAGA 
CCCACACGCA AGAGGAAGAG CGGCGAGATG GACAAGGATG CGGTGGAAGG TGAAAACATC 
AGAAATACAA GGTGCAGATT GACTCCTCAC CTAACACCTC ATCGGGAAGA GTCCCCAAAT 
TCCCAGGAGT CAAACCAAAG AGGGAAAAAA GACAAAACGC TCCAGAAGAT TGGAGATTTT 
CTTCAGAGCT CTCCCACCTT TCTTGGGAGT AAAGCGAAGA GGATCATGGG ATTGATGAGT 
GGAAAATCCC CTGATGGTGG GTCGACTCCT TCTCTAAAAC TGAAATCCAA ACGTGATAAA 
CGGAAATATG ACAGACCCGA TATCTCCTCA CCAATGGACA TACCGGCTCA TCGGATTATT 
GGCCGAAATC CAGAGGACAA AGAGAGTGAA AACCTTATCA AGAAACGTCT TCGTACAAGA 
ATGGCCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328820.1
CDS	149..5200
Translation	MMESGLKDDPARPAILVVEDLRRNLSTEFDCSDLQDSSDLKEHLKVYLRIRPFSAVEIENGESQDCVTIQRPDTVLLKPPQTSLSARLSDKSVPVTAQRFQFSQVYGPDTTQKDIFDGTVNGLVRNVLEGQNSLVFTYGVTNAGKTFTFLGPDTNPGILPRSLNMIFSSLEGRIFTQMCLKPQRCRDFIRLTKEQQNEEAASKRNLLKLFKESDAQRSISGHCSKSDIEDGSMLSDVNSAVLDVEMDMHTRFSIWVSFCEIYNENIHDLLEQHASNASRKTNLRLCQDVKGNSFIKDLKWVQVSSAEEALKVMKLGKRNQSISSTKLNLLSSRSHSIFSVRILKVEDVGIPRVESVSELALCDLAGSERCAKTQNKGERLKEAGNINTSLLSLGKCINALRNNQQARQHVPFRESKLTHYLQGYFTGRGSACMIVNINQCSSMYDETLNVLKFSAVAQKVVVLTTKNIPVGVKRSAREVSFIINNADCREMRRSSLVRWDPSLEDVQEDSDSEDEYEDQSEIEDTLLNSEDECTETVQINKKLYEGQQRLIVNTQQQLTAVQAELLTLEARVRDEVTKEFSELISQMQEDYSERMSRERELIEERCERRLEILKNLVEKTAMEDDRMNSTVAKSEEEKRVSLDSLFDSMSGDLAGIRHDAQAAQSCLVTSDPRVSATLHDLEKKVTELSEQLSISQNQLLLKTKELETQSRLSDELEEVKKTLESHREREFNLSDMCRQKDEMIAKLQATLDQHVERKAEDRAQMDSVQKEVIQCCCTIKEPLSSRNETRKRRQEGEGLEGQPPLKKGPPEDLKMPEDEADDGREDSALHEVKGQLECCLAEAKQKDKQITDLEQECHRLQVCIQDQKSNLDERVSQEHILKEDVCQTTLESKQMATAVDFSNLNVAVASRCSNTTGAFAAKSLMAKRDLELREKNKHILTLEKEIALMRQKAEHTSDYENLKKLNSELQAEVTSLCKVKAELEEKITCLQKEKEGLEDKEVGLTNPNTLGFKSKERDNQTHLMLSGKEAELALRENALTLKETQLSALQKSLKQTQERLEEEEMQAVQEARRKEVERRREILAVAEEAIAQKDAELQKRQVEISRLKEEIKTSEFKMSSLTVDLRRREDDSSDLREKLCDSKKQIQQVQKEISSMREAEKSLRLKLSDLEKTKVQLQNEIANRDRTINQLKAERSSGSKSDENLLLYQKACKDLQERERVIEDMRLALTEQEETQTQLDLELDNRDARIDELTQELASLREMLPEERDRSDSRPISKDVLQARQQITQAHESLQLANEKQQAERKKWMEEKLILIGQAKEAEERRNQDMRRFADDRERHVRQQAEMESLAARLNAREEEMERWRKERDTLVSALEVQLKKLIMSNGEKDQQIKMLQSSNTAQTPEEVSEDSRTEALQAELAVKEAEIQKLKEQLSISATNSSIRRNSTTQTMEVEFSDMKPLNSSTIKHKSRTRGSVISQNSSGSGPLVLDSSEISTETGRRSRFPRPELEISFSPLQPDRFALKRQGEESAVTVKISRPTRKRKSGEMDKDAVEGENIRNTRCRLTPHLTPHREESPNSQESNQRGKKDKTLQKIGDFLQSSPTFLGSKAKRIMGLMSGKSPDGGSTPSLKLKSKRDKRKYDRPDISSPMDIPAHRIIGRNPEDKESENLIKKRLRTRMAK

Target ORF information:

RefSeq Version	XM_021473145.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 20Ba (kif20ba), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473145.1

ORF Insert Sequence:

ATGATGGAGT CAGGGCTAAA GGATGATCCA GCAAGACCTG CTATATTAGT TGTAGAAGAT 
CTAAGGAGAA ATTTGTCCAC AGAGTTTGAT TGTTCAGATT TGCAGGACTC GTCAGACTTG 
AAGGAACATC TGAAGGTGTA TCTTAGGATA AGACCATTTT CAGCAGTTGA GATTGAGAAT 
GGAGAATCAC AGGACTGTGT GACCATTCAG CGTCCAGACA CTGTCCTTCT CAAACCTCCA 
CAAACATCTC TGTCTGCCAG GCTCAGTGAT AAATCAGTGC CAGTTACAGC TCAGCGTTTC 
CAGTTCTCTC AGGTCTATGG TCCAGACACT ACACAGAAGG ATATATTTGA TGGCACAGTA 
AACGGACTTG TCAGAAATGT TCTAGAAGGG CAAAATTCTC TGGTCTTTAC GTATGGTGTC 
ACCAATGCTG GGAAGACCTT TACTTTTTTA GGTCCAGACA CTAATCCGGG CATCTTGCCT 
CGTTCCCTCA ACATGATCTT CAGTAGTCTG GAGGGTCGCA TCTTTACCCA GATGTGCCTG 
AAACCCCAGA GGTGCCGAGA TTTCATCAGA CTTACAAAAG AGCAACAAAA TGAGGAAGCA 
GCCAGCAAAC GCAACCTGCT CAAGCTTTTC AAAGAGTCTG ATGCTCAGAG GAGTATTTCT 
GGCCATTGCT CAAAGTCTGA TATTGAAGAT GGTTCCATGC TCTCTGACGT AAACTCTGCG 
GTTTTGGATG TGGAGATGGA CATGCACACT AGGTTTTCTA TTTGGGTCTC GTTCTGTGAG 
ATCTACAATG AGAACATCCA TGACTTGCTA GAACAGCATG CAAGCAATGC TTCACGGAAA 
ACCAACCTTC GCCTTTGTCA GGATGTCAAA GGAAACTCAT TCATTAAAGA TCTAAAATGG 
GTCCAGGTGA GCAGTGCGGA GGAAGCCCTG AAAGTGATGA AACTGGGCAA AAGAAATCAG 
AGCATCTCTA GCACTAAACT CAACCTGCTC TCCAGCAGAA GCCACAGTAT TTTCTCTGTT 
CGAATACTGA AGGTGGAGGA TGTGGGAATA CCTAGAGTGG AGTCAGTCAG CGAGCTGGCA 
CTGTGTGATC TGGCTGGATC AGAGCGTTGT GCTAAGACTC AAAATAAAGG TGAACGGCTA 
AAAGAGGCTG GCAACATCAA TACTTCTCTG CTCAGTCTTG GAAAGTGCAT TAATGCACTA 
AGGAATAACC AACAGGCTAG ACAGCATGTT CCGTTCAGAG AGAGTAAACT CACTCACTAT 
CTGCAGGGCT ACTTCACAGG CCGGGGCTCA GCCTGTATGA TTGTAAACAT TAATCAGTGC 
TCCTCCATGT ATGATGAGAC GCTGAATGTG CTCAAGTTCT CTGCCGTGGC TCAGAAGGTG 
GTGGTCCTCA CCACTAAGAA CATCCCAGTG GGAGTGAAAA GGAGTGCAAG AGAGGTGTCC 
TTCATAATCA ACAACGCTGA TTGCCGGGAA ATGAGACGCA GCTCATTAGT ACGATGGGAC 
CCAAGTCTTG AGGATGTCCA GGAAGATTCT GACTCAGAGG ACGAGTATGA AGACCAGAGC 
GAAATCGAGG ACACCTTACT AAATTCTGAA GATGAATGTA CAGAGACTGT GCAAATCAAT 
AAAAAGCTTT ATGAGGGCCA GCAGCGGTTA ATAGTGAATA CTCAGCAGCA GTTGACAGCA 
GTACAAGCCG AACTCTTAAC CCTAGAAGCA CGTGTTCGAG ATGAAGTGAC AAAAGAATTC 
TCAGAACTGA TTTCCCAGAT GCAGGAAGAT TACAGTGAAC GTATGTCCAG AGAGCGGGAG 
CTGATAGAGG AACGGTGTGA ACGGAGACTG GAGATTCTCA AAAACCTAGT GGAAAAAACG 
GCTATGGAGG ATGACCGTAT GAATTCAACT GTGGCCAAAT CAGAGGAGGA GAAACGTGTC 
TCTCTAGACA GCCTGTTTGA CTCTATGAGT GGTGACCTGG CAGGAATCAG ACATGATGCC 
CAGGCTGCCC AGTCCTGCTT GGTGACCTCT GATCCCAGAG TCTCAGCGAC ATTGCATGAC 
CTTGAGAAAA AGGTCACAGA ACTTTCAGAA CAGCTCAGCA TATCTCAGAA TCAGCTGTTG 
CTCAAAACAA AGGAGCTTGA AACCCAGAGC CGGCTGTCAG ATGAACTGGA GGAGGTGAAG 
AAGACTCTGG AGAGCCACAG GGAAAGAGAA TTTAATCTGA GTGACATGTG CAGACAGAAG 
GATGAGATGA TTGCCAAACT CCAGGCTACT TTGGACCAGC ATGTAGAAAG AAAGGCTGAG 
GATAGAGCTC AAATGGACTC TGTCCAAAAG GAGGTCATCC AGTGCTGTTG CACCATTAAA 
GAGCCCCTCA GCTCCCGGAA TGAAACCAGA AAGCGACGTC AGGAAGGAGA GGGGCTTGAA 
GGACAGCCCC CGCTCAAGAA AGGCCCCCCG GAGGACTTGA AAATGCCTGA AGATGAAGCA 
GATGATGGGC GGGAAGACTC CGCCCTTCAT GAGGTCAAAG GTCAGCTGGA GTGTTGCCTT 
GCTGAAGCCA AACAAAAAGA CAAGCAGATC ACAGACCTTG AGCAGGAATG CCACAGATTA 
CAAGTGTGCA TTCAGGATCA AAAGTCAAAT CTGGATGAAA GAGTGTCACA GGAACATATT 
CTGAAAGAAG ACGTTTGTCA GACAACATTG GAAAGCAAAC AGATGGCAAC GGCTGTGGAT 
TTTAGCAATC TGAACGTAGC AGTTGCATCC AGATGTAGCA ACACTACAGG TGCGTTTGCT 
GCTAAATCAC TAATGGCTAA GCGAGATCTT GAACTGAGAG AAAAGAACAA ACACATCTTG 
ACTCTTGAAA AGGAAATTGC GCTAATGAGA CAGAAAGCAG AACACACATC TGACTATGAA 
AATCTCAAAA AACTCAACTC TGAACTGCAA GCTGAGGTCA CGAGCTTATG TAAGGTCAAA 
GCCGAACTTG AGGAGAAGAT TACATGCCTT CAGAAGGAGA AGGAGGGGCT TGAAGATAAG 
GAGGTGGGGC TGACAAACCC TAACACTCTT GGATTTAAAA GTAAAGAGAG AGATAACCAA 
ACACACCTCA TGCTGTCTGG AAAGGAGGCT GAACTTGCAC TGAGAGAGAA TGCTCTAACT 
TTAAAGGAAA CTCAGCTTTC AGCACTACAG AAGAGTCTGA AGCAAACACA GGAGCGTCTG 
GAGGAGGAGG AGATGCAGGC GGTGCAGGAG GCGCGCAGGA AGGAGGTGGA GAGGAGGAGA 
GAGATCCTGG CTGTGGCAGA GGAGGCCATC GCTCAGAAAG ATGCAGAGCT ACAGAAGAGA 
CAAGTAGAGA TCAGCCGGTT AAAAGAGGAG ATTAAAACTA GCGAGTTTAA GATGAGCAGT 
CTGACTGTTG ATCTGCGCAG AAGGGAAGAT GATTCATCTG ATCTTCGGGA AAAACTTTGT 
GACTCAAAGA AACAAATACA GCAGGTCCAG AAAGAGATTT CGTCCATGCG TGAAGCTGAG 
AAATCTCTAC GACTGAAGCT CAGCGATCTA GAAAAAACGA AAGTGCAGCT CCAGAATGAG 
ATCGCCAACA GAGATCGCAC CATTAACCAG TTAAAAGCTG AACGATCCTC TGGTTCAAAG 
TCAGATGAGA ATCTGCTGCT CTATCAGAAA GCATGTAAAG ATCTGCAAGA GCGTGAGCGT 
GTTATTGAAG ACATGCGCTT GGCTCTGACA GAACAGGAGG AAACACAGAC ACAGCTGGAC 
CTTGAGTTGG ACAACAGAGA CGCTCGGATT GATGAGCTCA CTCAAGAGCT GGCCAGTTTG 
AGAGAAATGT TGCCTGAAGA GAGGGACAGG AGTGATTCCA GACCAATCTC TAAAGATGTT 
TTACAGGCCA GACAGCAGAT CACTCAGGCC CACGAGAGCT TACAGCTTGC CAATGAGAAG 
CAGCAGGCAG AACGCAAGAA GTGGATGGAG GAGAAGTTGA TTCTGATTGG TCAGGCTAAA 
GAAGCTGAGG AGAGGCGGAA TCAGGACATG AGACGATTTG CAGATGATCG AGAACGGCAT 
GTTCGGCAAC AGGCTGAAAT GGAGTCTCTG GCTGCACGTC TTAATGCCCG TGAGGAAGAG 
ATGGAAAGGT GGAGAAAGGA GAGAGACACG CTTGTCTCTG CTCTGGAGGT TCAGCTGAAG 
AAACTCATCA TGTCCAATGG AGAAAAAGAC CAACAGATCA AGATGCTGCA GTCCAGTAAC 
ACTGCCCAAA CACCAGAGGA GGTCAGTGAG GACAGCAGGA CCGAGGCACT GCAGGCAGAG 
CTGGCTGTAA AAGAGGCTGA GATCCAGAAA CTGAAGGAGC AGCTCAGCAT CAGTGCCACA 
AACAGCAGCA TTCGCAGAAA CTCTACTACA CAGACGATGG AAGTGGAGTT TTCTGATATG 
AAGCCTCTGA ACAGCAGTAC AATCAAGCAC AAAAGCCGAA CCAGAGGATC TGTCATCAGT 
CAGAACTCAT CCGGTAGCGG TCCACTGGTG CTAGATTCAT CAGAGATCTC CACGGAAACA 
GGCAGACGGT CTAGGTTTCC AAGGCCTGAG CTGGAGATTT CCTTCAGTCC TCTACAGCCC 
GATCGCTTCG CTCTCAAGCG TCAGGGTGAA GAATCTGCTG TCACTGTTAA AATCTCCAGA 
CCCACACGCA AGAGGAAGAG CGGCGAGATG GACAAGGATG CGGTGGAAGG TGAAAACATC 
AGAAATACAA GGTGCAGATT GACTCCTCAC CTAACACCTC ATCGGGAAGA GTCCCCAAAT 
TCCCAGGAGT CAAACCAAAG AGGGAAAAAA GACAAAACGC TCCAGAAGAT TGGAGATTTT 
CTTCAGAGCT CTCCCACCTT TCTTGGGAGT AAAGCGAAGA GGATCATGGG ATTGATGAGT 
GGAAAATCCC CTGATGGTGG GTCGACTCCT TCTCTAAAAC TGAAATCCAA ACGTGATAAA 
CGGAAATATG ACAGACCCGA TATCTCCTCA CCAATGGACA TACCGGCTCA TCGGATTATT 
GGCCGAAATC CAGAGGACAA AGAGAGTGAA AACCTTATCA AGAAACGTCT TCGTACAAGA 
ATGGCCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif20ba ( XM_021474377.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330052.1 Danio rerio kinesin family member 20Ba (kif20ba), transcript variant X2, mRNA.

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CloneID	ODa22506
Clone ID Related Accession (Same CDS sequence)	XM_005158528.3 , XM_021473145.1 , XM_021474377.1 , XM_005158528.4
Accession Version	XM_021474377.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF20B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGT CAGGGCTAAA GGATGATCCA GCAAGACCTG CTATATTAGT TGTAGAAGAT 
CTAAGGAGAA ATTTGTCCAC AGAGTTTGAT TGTTCAGATT TGCAGGACTC GTCAGACTTG 
AAGGAACATC TGAAGGTGTA TCTTAGGATA AGACCATTTT CAGCAGTTGA GATTGAGAAT 
GGAGAATCAC AGGACTGTGT GACCATTCAG CGTCCAGACA CTGTCCTTCT CAAACCTCCA 
CAAACATCTC TGTCTGCCAG GCTCAGTGAT AAATCAGTGC CAGTTACAGC TCAGCGTTTC 
CAGTTCTCTC AGGTCTATGG TCCAGACACT ACACAGAAGG ATATATTTGA TGGCACAGTA 
AACGGACTTG TCAGAAATGT TCTAGAAGGG CAAAATTCTC TGGTCTTTAC GTATGGTGTC 
ACCAATGCTG GGAAGACCTT TACTTTTTTA GGTCCAGACA CTAATCCGGG CATCTTGCCT 
CGTTCCCTCA ACATGATCTT CAGTAGTCTG GAGGGTCGCA TCTTTACCCA GATGTGCCTG 
AAACCCCAGA GGTGCCGAGA TTTCATCAGA CTTACAAAAG AGCAACAAAA TGAGGAAGCA 
GCCAGCAAAC GCAACCTGCT CAAGCTTTTC AAAGAGTCTG ATGCTCAGAG GAGTATTTCT 
GGCCATTGCT CAAAGTCTGA TATTGAAGAT GGTTCCATGC TCTCTGACGT AAACTCTGCG 
GTTTTGGATG TGGAGATGGA CATGCACACT AGGTTTTCTA TTTGGGTCTC GTTCTGTGAG 
ATCTACAATG AGAACATCCA TGACTTGCTA GAACAGCATG CAAGCAATGC TTCACGGAAA 
ACCAACCTTC GCCTTTGTCA GGATGTCAAA GGAAACTCAT TCATTAAAGA TCTAAAATGG 
GTCCAGGTGA GCAGTGCGGA GGAAGCCCTG AAAGTGATGA AACTGGGCAA AAGAAATCAG 
AGCATCTCTA GCACTAAACT CAACCTGCTC TCCAGCAGAA GCCACAGTAT TTTCTCTGTT 
CGAATACTGA AGGTGGAGGA TGTGGGAATA CCTAGAGTGG AGTCAGTCAG CGAGCTGGCA 
CTGTGTGATC TGGCTGGATC AGAGCGTTGT GCTAAGACTC AAAATAAAGG TGAACGGCTA 
AAAGAGGCTG GCAACATCAA TACTTCTCTG CTCAGTCTTG GAAAGTGCAT TAATGCACTA 
AGGAATAACC AACAGGCTAG ACAGCATGTT CCGTTCAGAG AGAGTAAACT CACTCACTAT 
CTGCAGGGCT ACTTCACAGG CCGGGGCTCA GCCTGTATGA TTGTAAACAT TAATCAGTGC 
TCCTCCATGT ATGATGAGAC GCTGAATGTG CTCAAGTTCT CTGCCGTGGC TCAGAAGGTG 
GTGGTCCTCA CCACTAAGAA CATCCCAGTG GGAGTGAAAA GGAGTGCAAG AGAGGTGTCC 
TTCATAATCA ACAACGCTGA TTGCCGGGAA ATGAGACGCA GCTCATTAGT ACGATGGGAC 
CCAAGTCTTG AGGATGTCCA GGAAGATTCT GACTCAGAGG ACGAGTATGA AGACCAGAGC 
GAAATCGAGG ACACCTTACT AAATTCTGAA GATGAATGTA CAGAGACTGT GCAAATCAAT 
AAAAAGCTTT ATGAGGGCCA GCAGCGGTTA ATAGTGAATA CTCAGCAGCA GTTGACAGCA 
GTACAAGCCG AACTCTTAAC CCTAGAAGCA CGTGTTCGAG ATGAAGTGAC AAAAGAATTC 
TCAGAACTGA TTTCCCAGAT GCAGGAAGAT TACAGTGAAC GTATGTCCAG AGAGCGGGAG 
CTGATAGAGG AACGGTGTGA ACGGAGACTG GAGATTCTCA AAAACCTAGT GGAAAAAACG 
GCTATGGAGG ATGACCGTAT GAATTCAACT GTGGCCAAAT CAGAGGAGGA GAAACGTGTC 
TCTCTAGACA GCCTGTTTGA CTCTATGAGT GGTGACCTGG CAGGAATCAG ACATGATGCC 
CAGGCTGCCC AGTCCTGCTT GGTGACCTCT GATCCCAGAG TCTCAGCGAC ATTGCATGAC 
CTTGAGAAAA AGGTCACAGA ACTTTCAGAA CAGCTCAGCA TATCTCAGAA TCAGCTGTTG 
CTCAAAACAA AGGAGCTTGA AACCCAGAGC CGGCTGTCAG ATGAACTGGA GGAGGTGAAG 
AAGACTCTGG AGAGCCACAG GGAAAGAGAA TTTAATCTGA GTGACATGTG CAGACAGAAG 
GATGAGATGA TTGCCAAACT CCAGGCTACT TTGGACCAGC ATGTAGAAAG AAAGGCTGAG 
GATAGAGCTC AAATGGACTC TGTCCAAAAG GAGGTCATCC AGTGCTGTTG CACCATTAAA 
GAGCCCCTCA GCTCCCGGAA TGAAACCAGA AAGCGACGTC AGGAAGGAGA GGGGCTTGAA 
GGACAGCCCC CGCTCAAGAA AGGCCCCCCG GAGGACTTGA AAATGCCTGA AGATGAAGCA 
GATGATGGGC GGGAAGACTC CGCCCTTCAT GAGGTCAAAG GTCAGCTGGA GTGTTGCCTT 
GCTGAAGCCA AACAAAAAGA CAAGCAGATC ACAGACCTTG AGCAGGAATG CCACAGATTA 
CAAGTGTGCA TTCAGGATCA AAAGTCAAAT CTGGATGAAA GAGTGTCACA GGAACATATT 
CTGAAAGAAG ACGTTTGTCA GACAACATTG GAAAGCAAAC AGATGGCAAC GGCTGTGGAT 
TTTAGCAATC TGAACGTAGC AGTTGCATCC AGATGTAGCA ACACTACAGG TGCGTTTGCT 
GCTAAATCAC TAATGGCTAA GCGAGATCTT GAACTGAGAG AAAAGAACAA ACACATCTTG 
ACTCTTGAAA AGGAAATTGC GCTAATGAGA CAGAAAGCAG AACACACATC TGACTATGAA 
AATCTCAAAA AACTCAACTC TGAACTGCAA GCTGAGGTCA CGAGCTTATG TAAGGTCAAA 
GCCGAACTTG AGGAGAAGAT TACATGCCTT CAGAAGGAGA AGGAGGGGCT TGAAGATAAG 
GAGGTGGGGC TGACAAACCC TAACACTCTT GGATTTAAAA GTAAAGAGAG AGATAACCAA 
ACACACCTCA TGCTGTCTGG AAAGGAGGCT GAACTTGCAC TGAGAGAGAA TGCTCTAACT 
TTAAAGGAAA CTCAGCTTTC AGCACTACAG AAGAGTCTGA AGCAAACACA GGAGCGTCTG 
GAGGAGGAGG AGATGCAGGC GGTGCAGGAG GCGCGCAGGA AGGAGGTGGA GAGGAGGAGA 
GAGATCCTGG CTGTGGCAGA GGAGGCCATC GCTCAGAAAG ATGCAGAGCT ACAGAAGAGA 
CAAGTAGAGA TCAGCCGGTT AAAAGAGGAG ATTAAAACTA GCGAGTTTAA GATGAGCAGT 
CTGACTGTTG ATCTGCGCAG AAGGGAAGAT GATTCATCTG ATCTTCGGGA AAAACTTTGT 
GACTCAAAGA AACAAATACA GCAGGTCCAG AAAGAGATTT CGTCCATGCG TGAAGCTGAG 
AAATCTCTAC GACTGAAGCT CAGCGATCTA GAAAAAACGA AAGTGCAGCT CCAGAATGAG 
ATCGCCAACA GAGATCGCAC CATTAACCAG TTAAAAGCTG AACGATCCTC TGGTTCAAAG 
TCAGATGAGA ATCTGCTGCT CTATCAGAAA GCATGTAAAG ATCTGCAAGA GCGTGAGCGT 
GTTATTGAAG ACATGCGCTT GGCTCTGACA GAACAGGAGG AAACACAGAC ACAGCTGGAC 
CTTGAGTTGG ACAACAGAGA CGCTCGGATT GATGAGCTCA CTCAAGAGCT GGCCAGTTTG 
AGAGAAATGT TGCCTGAAGA GAGGGACAGG AGTGATTCCA GACCAATCTC TAAAGATGTT 
TTACAGGCCA GACAGCAGAT CACTCAGGCC CACGAGAGCT TACAGCTTGC CAATGAGAAG 
CAGCAGGCAG AACGCAAGAA GTGGATGGAG GAGAAGTTGA TTCTGATTGG TCAGGCTAAA 
GAAGCTGAGG AGAGGCGGAA TCAGGACATG AGACGATTTG CAGATGATCG AGAACGGCAT 
GTTCGGCAAC AGGCTGAAAT GGAGTCTCTG GCTGCACGTC TTAATGCCCG TGAGGAAGAG 
ATGGAAAGGT GGAGAAAGGA GAGAGACACG CTTGTCTCTG CTCTGGAGGT TCAGCTGAAG 
AAACTCATCA TGTCCAATGG AGAAAAAGAC CAACAGATCA AGATGCTGCA GTCCAGTAAC 
ACTGCCCAAA CACCAGAGGA GGTCAGTGAG GACAGCAGGA CCGAGGCACT GCAGGCAGAG 
CTGGCTGTAA AAGAGGCTGA GATCCAGAAA CTGAAGGAGC AGCTCAGCAT CAGTGCCACA 
AACAGCAGCA TTCGCAGAAA CTCTACTACA CAGACGATGG AAGTGGAGTT TTCTGATATG 
AAGCCTCTGA ACAGCAGTAC AATCAAGCAC AAAAGCCGAA CCAGAGGATC TGTCATCAGT 
CAGAACTCAT CCGGTAGCGG TCCACTGGTG CTAGATTCAT CAGAGATCTC CACGGAAACA 
GGCAGACGGT CTAGGTTTCC AAGGCCTGAG CTGGAGATTT CCTTCAGTCC TCTACAGCCC 
GATCGCTTCG CTCTCAAGCG TCAGGGTGAA GAATCTGCTG TCACTGTTAA AATCTCCAGA 
CCCACACGCA AGAGGAAGAG CGGCGAGATG GACAAGGATG CGGTGGAAGG TGAAAACATC 
AGAAATACAA GGTGCAGATT GACTCCTCAC CTAACACCTC ATCGGGAAGA GTCCCCAAAT 
TCCCAGGAGT CAAACCAAAG AGGGAAAAAA GACAAAACGC TCCAGAAGAT TGGAGATTTT 
CTTCAGAGCT CTCCCACCTT TCTTGGGAGT AAAGCGAAGA GGATCATGGG ATTGATGAGT 
GGAAAATCCC CTGATGGTGG GTCGACTCCT TCTCTAAAAC TGAAATCCAA ACGTGATAAA 
CGGAAATATG ACAGACCCGA TATCTCCTCA CCAATGGACA TACCGGCTCA TCGGATTATT 
GGCCGAAATC CAGAGGACAA AGAGAGTGAA AACCTTATCA AGAAACGTCT TCGTACAAGA 
ATGGCCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330052.1
CDS	149..5200
Translation	MMESGLKDDPARPAILVVEDLRRNLSTEFDCSDLQDSSDLKEHLKVYLRIRPFSAVEIENGESQDCVTIQRPDTVLLKPPQTSLSARLSDKSVPVTAQRFQFSQVYGPDTTQKDIFDGTVNGLVRNVLEGQNSLVFTYGVTNAGKTFTFLGPDTNPGILPRSLNMIFSSLEGRIFTQMCLKPQRCRDFIRLTKEQQNEEAASKRNLLKLFKESDAQRSISGHCSKSDIEDGSMLSDVNSAVLDVEMDMHTRFSIWVSFCEIYNENIHDLLEQHASNASRKTNLRLCQDVKGNSFIKDLKWVQVSSAEEALKVMKLGKRNQSISSTKLNLLSSRSHSIFSVRILKVEDVGIPRVESVSELALCDLAGSERCAKTQNKGERLKEAGNINTSLLSLGKCINALRNNQQARQHVPFRESKLTHYLQGYFTGRGSACMIVNINQCSSMYDETLNVLKFSAVAQKVVVLTTKNIPVGVKRSAREVSFIINNADCREMRRSSLVRWDPSLEDVQEDSDSEDEYEDQSEIEDTLLNSEDECTETVQINKKLYEGQQRLIVNTQQQLTAVQAELLTLEARVRDEVTKEFSELISQMQEDYSERMSRERELIEERCERRLEILKNLVEKTAMEDDRMNSTVAKSEEEKRVSLDSLFDSMSGDLAGIRHDAQAAQSCLVTSDPRVSATLHDLEKKVTELSEQLSISQNQLLLKTKELETQSRLSDELEEVKKTLESHREREFNLSDMCRQKDEMIAKLQATLDQHVERKAEDRAQMDSVQKEVIQCCCTIKEPLSSRNETRKRRQEGEGLEGQPPLKKGPPEDLKMPEDEADDGREDSALHEVKGQLECCLAEAKQKDKQITDLEQECHRLQVCIQDQKSNLDERVSQEHILKEDVCQTTLESKQMATAVDFSNLNVAVASRCSNTTGAFAAKSLMAKRDLELREKNKHILTLEKEIALMRQKAEHTSDYENLKKLNSELQAEVTSLCKVKAELEEKITCLQKEKEGLEDKEVGLTNPNTLGFKSKERDNQTHLMLSGKEAELALRENALTLKETQLSALQKSLKQTQERLEEEEMQAVQEARRKEVERRREILAVAEEAIAQKDAELQKRQVEISRLKEEIKTSEFKMSSLTVDLRRREDDSSDLREKLCDSKKQIQQVQKEISSMREAEKSLRLKLSDLEKTKVQLQNEIANRDRTINQLKAERSSGSKSDENLLLYQKACKDLQERERVIEDMRLALTEQEETQTQLDLELDNRDARIDELTQELASLREMLPEERDRSDSRPISKDVLQARQQITQAHESLQLANEKQQAERKKWMEEKLILIGQAKEAEERRNQDMRRFADDRERHVRQQAEMESLAARLNAREEEMERWRKERDTLVSALEVQLKKLIMSNGEKDQQIKMLQSSNTAQTPEEVSEDSRTEALQAELAVKEAEIQKLKEQLSISATNSSIRRNSTTQTMEVEFSDMKPLNSSTIKHKSRTRGSVISQNSSGSGPLVLDSSEISTETGRRSRFPRPELEISFSPLQPDRFALKRQGEESAVTVKISRPTRKRKSGEMDKDAVEGENIRNTRCRLTPHLTPHREESPNSQESNQRGKKDKTLQKIGDFLQSSPTFLGSKAKRIMGLMSGKSPDGGSTPSLKLKSKRDKRKYDRPDISSPMDIPAHRIIGRNPEDKESENLIKKRLRTRMAK

Target ORF information:

RefSeq Version	XM_021474377.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 20Ba (kif20ba), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474377.1

ORF Insert Sequence:

ATGATGGAGT CAGGGCTAAA GGATGATCCA GCAAGACCTG CTATATTAGT TGTAGAAGAT 
CTAAGGAGAA ATTTGTCCAC AGAGTTTGAT TGTTCAGATT TGCAGGACTC GTCAGACTTG 
AAGGAACATC TGAAGGTGTA TCTTAGGATA AGACCATTTT CAGCAGTTGA GATTGAGAAT 
GGAGAATCAC AGGACTGTGT GACCATTCAG CGTCCAGACA CTGTCCTTCT CAAACCTCCA 
CAAACATCTC TGTCTGCCAG GCTCAGTGAT AAATCAGTGC CAGTTACAGC TCAGCGTTTC 
CAGTTCTCTC AGGTCTATGG TCCAGACACT ACACAGAAGG ATATATTTGA TGGCACAGTA 
AACGGACTTG TCAGAAATGT TCTAGAAGGG CAAAATTCTC TGGTCTTTAC GTATGGTGTC 
ACCAATGCTG GGAAGACCTT TACTTTTTTA GGTCCAGACA CTAATCCGGG CATCTTGCCT 
CGTTCCCTCA ACATGATCTT CAGTAGTCTG GAGGGTCGCA TCTTTACCCA GATGTGCCTG 
AAACCCCAGA GGTGCCGAGA TTTCATCAGA CTTACAAAAG AGCAACAAAA TGAGGAAGCA 
GCCAGCAAAC GCAACCTGCT CAAGCTTTTC AAAGAGTCTG ATGCTCAGAG GAGTATTTCT 
GGCCATTGCT CAAAGTCTGA TATTGAAGAT GGTTCCATGC TCTCTGACGT AAACTCTGCG 
GTTTTGGATG TGGAGATGGA CATGCACACT AGGTTTTCTA TTTGGGTCTC GTTCTGTGAG 
ATCTACAATG AGAACATCCA TGACTTGCTA GAACAGCATG CAAGCAATGC TTCACGGAAA 
ACCAACCTTC GCCTTTGTCA GGATGTCAAA GGAAACTCAT TCATTAAAGA TCTAAAATGG 
GTCCAGGTGA GCAGTGCGGA GGAAGCCCTG AAAGTGATGA AACTGGGCAA AAGAAATCAG 
AGCATCTCTA GCACTAAACT CAACCTGCTC TCCAGCAGAA GCCACAGTAT TTTCTCTGTT 
CGAATACTGA AGGTGGAGGA TGTGGGAATA CCTAGAGTGG AGTCAGTCAG CGAGCTGGCA 
CTGTGTGATC TGGCTGGATC AGAGCGTTGT GCTAAGACTC AAAATAAAGG TGAACGGCTA 
AAAGAGGCTG GCAACATCAA TACTTCTCTG CTCAGTCTTG GAAAGTGCAT TAATGCACTA 
AGGAATAACC AACAGGCTAG ACAGCATGTT CCGTTCAGAG AGAGTAAACT CACTCACTAT 
CTGCAGGGCT ACTTCACAGG CCGGGGCTCA GCCTGTATGA TTGTAAACAT TAATCAGTGC 
TCCTCCATGT ATGATGAGAC GCTGAATGTG CTCAAGTTCT CTGCCGTGGC TCAGAAGGTG 
GTGGTCCTCA CCACTAAGAA CATCCCAGTG GGAGTGAAAA GGAGTGCAAG AGAGGTGTCC 
TTCATAATCA ACAACGCTGA TTGCCGGGAA ATGAGACGCA GCTCATTAGT ACGATGGGAC 
CCAAGTCTTG AGGATGTCCA GGAAGATTCT GACTCAGAGG ACGAGTATGA AGACCAGAGC 
GAAATCGAGG ACACCTTACT AAATTCTGAA GATGAATGTA CAGAGACTGT GCAAATCAAT 
AAAAAGCTTT ATGAGGGCCA GCAGCGGTTA ATAGTGAATA CTCAGCAGCA GTTGACAGCA 
GTACAAGCCG AACTCTTAAC CCTAGAAGCA CGTGTTCGAG ATGAAGTGAC AAAAGAATTC 
TCAGAACTGA TTTCCCAGAT GCAGGAAGAT TACAGTGAAC GTATGTCCAG AGAGCGGGAG 
CTGATAGAGG AACGGTGTGA ACGGAGACTG GAGATTCTCA AAAACCTAGT GGAAAAAACG 
GCTATGGAGG ATGACCGTAT GAATTCAACT GTGGCCAAAT CAGAGGAGGA GAAACGTGTC 
TCTCTAGACA GCCTGTTTGA CTCTATGAGT GGTGACCTGG CAGGAATCAG ACATGATGCC 
CAGGCTGCCC AGTCCTGCTT GGTGACCTCT GATCCCAGAG TCTCAGCGAC ATTGCATGAC 
CTTGAGAAAA AGGTCACAGA ACTTTCAGAA CAGCTCAGCA TATCTCAGAA TCAGCTGTTG 
CTCAAAACAA AGGAGCTTGA AACCCAGAGC CGGCTGTCAG ATGAACTGGA GGAGGTGAAG 
AAGACTCTGG AGAGCCACAG GGAAAGAGAA TTTAATCTGA GTGACATGTG CAGACAGAAG 
GATGAGATGA TTGCCAAACT CCAGGCTACT TTGGACCAGC ATGTAGAAAG AAAGGCTGAG 
GATAGAGCTC AAATGGACTC TGTCCAAAAG GAGGTCATCC AGTGCTGTTG CACCATTAAA 
GAGCCCCTCA GCTCCCGGAA TGAAACCAGA AAGCGACGTC AGGAAGGAGA GGGGCTTGAA 
GGACAGCCCC CGCTCAAGAA AGGCCCCCCG GAGGACTTGA AAATGCCTGA AGATGAAGCA 
GATGATGGGC GGGAAGACTC CGCCCTTCAT GAGGTCAAAG GTCAGCTGGA GTGTTGCCTT 
GCTGAAGCCA AACAAAAAGA CAAGCAGATC ACAGACCTTG AGCAGGAATG CCACAGATTA 
CAAGTGTGCA TTCAGGATCA AAAGTCAAAT CTGGATGAAA GAGTGTCACA GGAACATATT 
CTGAAAGAAG ACGTTTGTCA GACAACATTG GAAAGCAAAC AGATGGCAAC GGCTGTGGAT 
TTTAGCAATC TGAACGTAGC AGTTGCATCC AGATGTAGCA ACACTACAGG TGCGTTTGCT 
GCTAAATCAC TAATGGCTAA GCGAGATCTT GAACTGAGAG AAAAGAACAA ACACATCTTG 
ACTCTTGAAA AGGAAATTGC GCTAATGAGA CAGAAAGCAG AACACACATC TGACTATGAA 
AATCTCAAAA AACTCAACTC TGAACTGCAA GCTGAGGTCA CGAGCTTATG TAAGGTCAAA 
GCCGAACTTG AGGAGAAGAT TACATGCCTT CAGAAGGAGA AGGAGGGGCT TGAAGATAAG 
GAGGTGGGGC TGACAAACCC TAACACTCTT GGATTTAAAA GTAAAGAGAG AGATAACCAA 
ACACACCTCA TGCTGTCTGG AAAGGAGGCT GAACTTGCAC TGAGAGAGAA TGCTCTAACT 
TTAAAGGAAA CTCAGCTTTC AGCACTACAG AAGAGTCTGA AGCAAACACA GGAGCGTCTG 
GAGGAGGAGG AGATGCAGGC GGTGCAGGAG GCGCGCAGGA AGGAGGTGGA GAGGAGGAGA 
GAGATCCTGG CTGTGGCAGA GGAGGCCATC GCTCAGAAAG ATGCAGAGCT ACAGAAGAGA 
CAAGTAGAGA TCAGCCGGTT AAAAGAGGAG ATTAAAACTA GCGAGTTTAA GATGAGCAGT 
CTGACTGTTG ATCTGCGCAG AAGGGAAGAT GATTCATCTG ATCTTCGGGA AAAACTTTGT 
GACTCAAAGA AACAAATACA GCAGGTCCAG AAAGAGATTT CGTCCATGCG TGAAGCTGAG 
AAATCTCTAC GACTGAAGCT CAGCGATCTA GAAAAAACGA AAGTGCAGCT CCAGAATGAG 
ATCGCCAACA GAGATCGCAC CATTAACCAG TTAAAAGCTG AACGATCCTC TGGTTCAAAG 
TCAGATGAGA ATCTGCTGCT CTATCAGAAA GCATGTAAAG ATCTGCAAGA GCGTGAGCGT 
GTTATTGAAG ACATGCGCTT GGCTCTGACA GAACAGGAGG AAACACAGAC ACAGCTGGAC 
CTTGAGTTGG ACAACAGAGA CGCTCGGATT GATGAGCTCA CTCAAGAGCT GGCCAGTTTG 
AGAGAAATGT TGCCTGAAGA GAGGGACAGG AGTGATTCCA GACCAATCTC TAAAGATGTT 
TTACAGGCCA GACAGCAGAT CACTCAGGCC CACGAGAGCT TACAGCTTGC CAATGAGAAG 
CAGCAGGCAG AACGCAAGAA GTGGATGGAG GAGAAGTTGA TTCTGATTGG TCAGGCTAAA 
GAAGCTGAGG AGAGGCGGAA TCAGGACATG AGACGATTTG CAGATGATCG AGAACGGCAT 
GTTCGGCAAC AGGCTGAAAT GGAGTCTCTG GCTGCACGTC TTAATGCCCG TGAGGAAGAG 
ATGGAAAGGT GGAGAAAGGA GAGAGACACG CTTGTCTCTG CTCTGGAGGT TCAGCTGAAG 
AAACTCATCA TGTCCAATGG AGAAAAAGAC CAACAGATCA AGATGCTGCA GTCCAGTAAC 
ACTGCCCAAA CACCAGAGGA GGTCAGTGAG GACAGCAGGA CCGAGGCACT GCAGGCAGAG 
CTGGCTGTAA AAGAGGCTGA GATCCAGAAA CTGAAGGAGC AGCTCAGCAT CAGTGCCACA 
AACAGCAGCA TTCGCAGAAA CTCTACTACA CAGACGATGG AAGTGGAGTT TTCTGATATG 
AAGCCTCTGA ACAGCAGTAC AATCAAGCAC AAAAGCCGAA CCAGAGGATC TGTCATCAGT 
CAGAACTCAT CCGGTAGCGG TCCACTGGTG CTAGATTCAT CAGAGATCTC CACGGAAACA 
GGCAGACGGT CTAGGTTTCC AAGGCCTGAG CTGGAGATTT CCTTCAGTCC TCTACAGCCC 
GATCGCTTCG CTCTCAAGCG TCAGGGTGAA GAATCTGCTG TCACTGTTAA AATCTCCAGA 
CCCACACGCA AGAGGAAGAG CGGCGAGATG GACAAGGATG CGGTGGAAGG TGAAAACATC 
AGAAATACAA GGTGCAGATT GACTCCTCAC CTAACACCTC ATCGGGAAGA GTCCCCAAAT 
TCCCAGGAGT CAAACCAAAG AGGGAAAAAA GACAAAACGC TCCAGAAGAT TGGAGATTTT 
CTTCAGAGCT CTCCCACCTT TCTTGGGAGT AAAGCGAAGA GGATCATGGG ATTGATGAGT 
GGAAAATCCC CTGATGGTGG GTCGACTCCT TCTCTAAAAC TGAAATCCAA ACGTGATAAA 
CGGAAATATG ACAGACCCGA TATCTCCTCA CCAATGGACA TACCGGCTCA TCGGATTATT 
GGCCGAAATC CAGAGGACAA AGAGAGTGAA AACCTTATCA AGAAACGTCT TCGTACAAGA 
ATGGCCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif20ba ( XM_005158528.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158585.1 Danio rerio kinesin family member 20Ba (kif20ba), transcript variant X2, mRNA.

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CloneID	ODa22506
Clone ID Related Accession (Same CDS sequence)	XM_005158528.3 , XM_021473145.1 , XM_021474377.1 , XM_005158528.4
Accession Version	XM_005158528.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF20B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158528.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGT CAGGGCTAAA GGATGATCCA GCAAGACCTG CTATATTAGT TGTAGAAGAT 
CTAAGGAGAA ATTTGTCCAC AGAGTTTGAT TGTTCAGATT TGCAGGACTC GTCAGACTTG 
AAGGAACATC TGAAGGTGTA TCTTAGGATA AGACCATTTT CAGCAGTTGA GATTGAGAAT 
GGAGAATCAC AGGACTGTGT GACCATTCAG CGTCCAGACA CTGTCCTTCT CAAACCTCCA 
CAAACATCTC TGTCTGCCAG GCTCAGTGAT AAATCAGTGC CAGTTACAGC TCAGCGTTTC 
CAGTTCTCTC AGGTCTATGG TCCAGACACT ACACAGAAGG ATATATTTGA TGGCACAGTA 
AACGGACTTG TCAGAAATGT TCTAGAAGGG CAAAATTCTC TGGTCTTTAC GTATGGTGTC 
ACCAATGCTG GGAAGACCTT TACTTTTTTA GGTCCAGACA CTAATCCGGG CATCTTGCCT 
CGTTCCCTCA ACATGATCTT CAGTAGTCTG GAGGGTCGCA TCTTTACCCA GATGTGCCTG 
AAACCCCAGA GGTGCCGAGA TTTCATCAGA CTTACAAAAG AGCAACAAAA TGAGGAAGCA 
GCCAGCAAAC GCAACCTGCT CAAGCTTTTC AAAGAGTCTG ATGCTCAGAG GAGTATTTCT 
GGCCATTGCT CAAAGTCTGA TATTGAAGAT GGTTCCATGC TCTCTGACGT AAACTCTGCG 
GTTTTGGATG TGGAGATGGA CATGCACACT AGGTTTTCTA TTTGGGTCTC GTTCTGTGAG 
ATCTACAATG AGAACATCCA TGACTTGCTA GAACAGCATG CAAGCAATGC TTCACGGAAA 
ACCAACCTTC GCCTTTGTCA GGATGTCAAA GGAAACTCAT TCATTAAAGA TCTAAAATGG 
GTCCAGGTGA GCAGTGCGGA GGAAGCCCTG AAAGTGATGA AACTGGGCAA AAGAAATCAG 
AGCATCTCTA GCACTAAACT CAACCTGCTC TCCAGCAGAA GCCACAGTAT TTTCTCTGTT 
CGAATACTGA AGGTGGAGGA TGTGGGAATA CCTAGAGTGG AGTCAGTCAG CGAGCTGGCA 
CTGTGTGATC TGGCTGGATC AGAGCGTTGT GCTAAGACTC AAAATAAAGG TGAACGGCTA 
AAAGAGGCTG GCAACATCAA TACTTCTCTG CTCAGTCTTG GAAAGTGCAT TAATGCACTA 
AGGAATAACC AACAGGCTAG ACAGCATGTT CCGTTCAGAG AGAGTAAACT CACTCACTAT 
CTGCAGGGCT ACTTCACAGG CCGGGGCTCA GCCTGTATGA TTGTAAACAT TAATCAGTGC 
TCCTCCATGT ATGATGAGAC GCTGAATGTG CTCAAGTTCT CTGCCGTGGC TCAGAAGGTG 
GTGGTCCTCA CCACTAAGAA CATCCCAGTG GGAGTGAAAA GGAGTGCAAG AGAGGTGTCC 
TTCATAATCA ACAACGCTGA TTGCCGGGAA ATGAGACGCA GCTCATTAGT ACGATGGGAC 
CCAAGTCTTG AGGATGTCCA GGAAGATTCT GACTCAGAGG ACGAGTATGA AGACCAGAGC 
GAAATCGAGG ACACCTTACT AAATTCTGAA GATGAATGTA CAGAGACTGT GCAAATCAAT 
AAAAAGCTTT ATGAGGGCCA GCAGCGGTTA ATAGTGAATA CTCAGCAGCA GTTGACAGCA 
GTACAAGCCG AACTCTTAAC CCTAGAAGCA CGTGTTCGAG ATGAAGTGAC AAAAGAATTC 
TCAGAACTGA TTTCCCAGAT GCAGGAAGAT TACAGTGAAC GTATGTCCAG AGAGCGGGAG 
CTGATAGAGG AACGGTGTGA ACGGAGACTG GAGATTCTCA AAAACCTAGT GGAAAAAACG 
GCTATGGAGG ATGACCGTAT GAATTCAACT GTGGCCAAAT CAGAGGAGGA GAAACGTGTC 
TCTCTAGACA GCCTGTTTGA CTCTATGAGT GGTGACCTGG CAGGAATCAG ACATGATGCC 
CAGGCTGCCC AGTCCTGCTT GGTGACCTCT GATCCCAGAG TCTCAGCGAC ATTGCATGAC 
CTTGAGAAAA AGGTCACAGA ACTTTCAGAA CAGCTCAGCA TATCTCAGAA TCAGCTGTTG 
CTCAAAACAA AGGAGCTTGA AACCCAGAGC CGGCTGTCAG ATGAACTGGA GGAGGTGAAG 
AAGACTCTGG AGAGCCACAG GGAAAGAGAA TTTAATCTGA GTGACATGTG CAGACAGAAG 
GATGAGATGA TTGCCAAACT CCAGGCTACT TTGGACCAGC ATGTAGAAAG AAAGGCTGAG 
GATAGAGCTC AAATGGACTC TGTCCAAAAG GAGGTCATCC AGTGCTGTTG CACCATTAAA 
GAGCCCCTCA GCTCCCGGAA TGAAACCAGA AAGCGACGTC AGGAAGGAGA GGGGCTTGAA 
GGACAGCCCC CGCTCAAGAA AGGCCCCCCG GAGGACTTGA AAATGCCTGA AGATGAAGCA 
GATGATGGGC GGGAAGACTC CGCCCTTCAT GAGGTCAAAG GTCAGCTGGA GTGTTGCCTT 
GCTGAAGCCA AACAAAAAGA CAAGCAGATC ACAGACCTTG AGCAGGAATG CCACAGATTA 
CAAGTGTGCA TTCAGGATCA AAAGTCAAAT CTGGATGAAA GAGTGTCACA GGAACATATT 
CTGAAAGAAG ACGTTTGTCA GACAACATTG GAAAGCAAAC AGATGGCAAC GGCTGTGGAT 
TTTAGCAATC TGAACGTAGC AGTTGCATCC AGATGTAGCA ACACTACAGG TGCGTTTGCT 
GCTAAATCAC TAATGGCTAA GCGAGATCTT GAACTGAGAG AAAAGAACAA ACACATCTTG 
ACTCTTGAAA AGGAAATTGC GCTAATGAGA CAGAAAGCAG AACACACATC TGACTATGAA 
AATCTCAAAA AACTCAACTC TGAACTGCAA GCTGAGGTCA CGAGCTTATG TAAGGTCAAA 
GCCGAACTTG AGGAGAAGAT TACATGCCTT CAGAAGGAGA AGGAGGGGCT TGAAGATAAG 
GAGGTGGGGC TGACAAACCC TAACACTCTT GGATTTAAAA GTAAAGAGAG AGATAACCAA 
ACACACCTCA TGCTGTCTGG AAAGGAGGCT GAACTTGCAC TGAGAGAGAA TGCTCTAACT 
TTAAAGGAAA CTCAGCTTTC AGCACTACAG AAGAGTCTGA AGCAAACACA GGAGCGTCTG 
GAGGAGGAGG AGATGCAGGC GGTGCAGGAG GCGCGCAGGA AGGAGGTGGA GAGGAGGAGA 
GAGATCCTGG CTGTGGCAGA GGAGGCCATC GCTCAGAAAG ATGCAGAGCT ACAGAAGAGA 
CAAGTAGAGA TCAGCCGGTT AAAAGAGGAG ATTAAAACTA GCGAGTTTAA GATGAGCAGT 
CTGACTGTTG ATCTGCGCAG AAGGGAAGAT GATTCATCTG ATCTTCGGGA AAAACTTTGT 
GACTCAAAGA AACAAATACA GCAGGTCCAG AAAGAGATTT CGTCCATGCG TGAAGCTGAG 
AAATCTCTAC GACTGAAGCT CAGCGATCTA GAAAAAACGA AAGTGCAGCT CCAGAATGAG 
ATCGCCAACA GAGATCGCAC CATTAACCAG TTAAAAGCTG AACGATCCTC TGGTTCAAAG 
TCAGATGAGA ATCTGCTGCT CTATCAGAAA GCATGTAAAG ATCTGCAAGA GCGTGAGCGT 
GTTATTGAAG ACATGCGCTT GGCTCTGACA GAACAGGAGG AAACACAGAC ACAGCTGGAC 
CTTGAGTTGG ACAACAGAGA CGCTCGGATT GATGAGCTCA CTCAAGAGCT GGCCAGTTTG 
AGAGAAATGT TGCCTGAAGA GAGGGACAGG AGTGATTCCA GACCAATCTC TAAAGATGTT 
TTACAGGCCA GACAGCAGAT CACTCAGGCC CACGAGAGCT TACAGCTTGC CAATGAGAAG 
CAGCAGGCAG AACGCAAGAA GTGGATGGAG GAGAAGTTGA TTCTGATTGG TCAGGCTAAA 
GAAGCTGAGG AGAGGCGGAA TCAGGACATG AGACGATTTG CAGATGATCG AGAACGGCAT 
GTTCGGCAAC AGGCTGAAAT GGAGTCTCTG GCTGCACGTC TTAATGCCCG TGAGGAAGAG 
ATGGAAAGGT GGAGAAAGGA GAGAGACACG CTTGTCTCTG CTCTGGAGGT TCAGCTGAAG 
AAACTCATCA TGTCCAATGG AGAAAAAGAC CAACAGATCA AGATGCTGCA GTCCAGTAAC 
ACTGCCCAAA CACCAGAGGA GGTCAGTGAG GACAGCAGGA CCGAGGCACT GCAGGCAGAG 
CTGGCTGTAA AAGAGGCTGA GATCCAGAAA CTGAAGGAGC AGCTCAGCAT CAGTGCCACA 
AACAGCAGCA TTCGCAGAAA CTCTACTACA CAGACGATGG AAGTGGAGTT TTCTGATATG 
AAGCCTCTGA ACAGCAGTAC AATCAAGCAC AAAAGCCGAA CCAGAGGATC TGTCATCAGT 
CAGAACTCAT CCGGTAGCGG TCCACTGGTG CTAGATTCAT CAGAGATCTC CACGGAAACA 
GGCAGACGGT CTAGGTTTCC AAGGCCTGAG CTGGAGATTT CCTTCAGTCC TCTACAGCCC 
GATCGCTTCG CTCTCAAGCG TCAGGGTGAA GAATCTGCTG TCACTGTTAA AATCTCCAGA 
CCCACACGCA AGAGGAAGAG CGGCGAGATG GACAAGGATG CGGTGGAAGG TGAAAACATC 
AGAAATACAA GGTGCAGATT GACTCCTCAC CTAACACCTC ATCGGGAAGA GTCCCCAAAT 
TCCCAGGAGT CAAACCAAAG AGGGAAAAAA GACAAAACGC TCCAGAAGAT TGGAGATTTT 
CTTCAGAGCT CTCCCACCTT TCTTGGGAGT AAAGCGAAGA GGATCATGGG ATTGATGAGT 
GGAAAATCCC CTGATGGTGG GTCGACTCCT TCTCTAAAAC TGAAATCCAA ACGTGATAAA 
CGGAAATATG ACAGACCCGA TATCTCCTCA CCAATGGACA TACCGGCTCA TCGGATTATT 
GGCCGAAATC CAGAGGACAA AGAGAGTGAA AACCTTATCA AGAAACGTCT TCGTACAAGA 
ATGGCCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158585.1
CDS	149..5200
Translation	MMESGLKDDPARPAILVVEDLRRNLSTEFDCSDLQDSSDLKEHLKVYLRIRPFSAVEIENGESQDCVTIQRPDTVLLKPPQTSLSARLSDKSVPVTAQRFQFSQVYGPDTTQKDIFDGTVNGLVRNVLEGQNSLVFTYGVTNAGKTFTFLGPDTNPGILPRSLNMIFSSLEGRIFTQMCLKPQRCRDFIRLTKEQQNEEAASKRNLLKLFKESDAQRSISGHCSKSDIEDGSMLSDVNSAVLDVEMDMHTRFSIWVSFCEIYNENIHDLLEQHASNASRKTNLRLCQDVKGNSFIKDLKWVQVSSAEEALKVMKLGKRNQSISSTKLNLLSSRSHSIFSVRILKVEDVGIPRVESVSELALCDLAGSERCAKTQNKGERLKEAGNINTSLLSLGKCINALRNNQQARQHVPFRESKLTHYLQGYFTGRGSACMIVNINQCSSMYDETLNVLKFSAVAQKVVVLTTKNIPVGVKRSAREVSFIINNADCREMRRSSLVRWDPSLEDVQEDSDSEDEYEDQSEIEDTLLNSEDECTETVQINKKLYEGQQRLIVNTQQQLTAVQAELLTLEARVRDEVTKEFSELISQMQEDYSERMSRERELIEERCERRLEILKNLVEKTAMEDDRMNSTVAKSEEEKRVSLDSLFDSMSGDLAGIRHDAQAAQSCLVTSDPRVSATLHDLEKKVTELSEQLSISQNQLLLKTKELETQSRLSDELEEVKKTLESHREREFNLSDMCRQKDEMIAKLQATLDQHVERKAEDRAQMDSVQKEVIQCCCTIKEPLSSRNETRKRRQEGEGLEGQPPLKKGPPEDLKMPEDEADDGREDSALHEVKGQLECCLAEAKQKDKQITDLEQECHRLQVCIQDQKSNLDERVSQEHILKEDVCQTTLESKQMATAVDFSNLNVAVASRCSNTTGAFAAKSLMAKRDLELREKNKHILTLEKEIALMRQKAEHTSDYENLKKLNSELQAEVTSLCKVKAELEEKITCLQKEKEGLEDKEVGLTNPNTLGFKSKERDNQTHLMLSGKEAELALRENALTLKETQLSALQKSLKQTQERLEEEEMQAVQEARRKEVERRREILAVAEEAIAQKDAELQKRQVEISRLKEEIKTSEFKMSSLTVDLRRREDDSSDLREKLCDSKKQIQQVQKEISSMREAEKSLRLKLSDLEKTKVQLQNEIANRDRTINQLKAERSSGSKSDENLLLYQKACKDLQERERVIEDMRLALTEQEETQTQLDLELDNRDARIDELTQELASLREMLPEERDRSDSRPISKDVLQARQQITQAHESLQLANEKQQAERKKWMEEKLILIGQAKEAEERRNQDMRRFADDRERHVRQQAEMESLAARLNAREEEMERWRKERDTLVSALEVQLKKLIMSNGEKDQQIKMLQSSNTAQTPEEVSEDSRTEALQAELAVKEAEIQKLKEQLSISATNSSIRRNSTTQTMEVEFSDMKPLNSSTIKHKSRTRGSVISQNSSGSGPLVLDSSEISTETGRRSRFPRPELEISFSPLQPDRFALKRQGEESAVTVKISRPTRKRKSGEMDKDAVEGENIRNTRCRLTPHLTPHREESPNSQESNQRGKKDKTLQKIGDFLQSSPTFLGSKAKRIMGLMSGKSPDGGSTPSLKLKSKRDKRKYDRPDISSPMDIPAHRIIGRNPEDKESENLIKKRLRTRMAK

Target ORF information:

RefSeq Version	XM_005158528.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 20Ba (kif20ba), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158528.4

ORF Insert Sequence:

ATGATGGAGT CAGGGCTAAA GGATGATCCA GCAAGACCTG CTATATTAGT TGTAGAAGAT 
CTAAGGAGAA ATTTGTCCAC AGAGTTTGAT TGTTCAGATT TGCAGGACTC GTCAGACTTG 
AAGGAACATC TGAAGGTGTA TCTTAGGATA AGACCATTTT CAGCAGTTGA GATTGAGAAT 
GGAGAATCAC AGGACTGTGT GACCATTCAG CGTCCAGACA CTGTCCTTCT CAAACCTCCA 
CAAACATCTC TGTCTGCCAG GCTCAGTGAT AAATCAGTGC CAGTTACAGC TCAGCGTTTC 
CAGTTCTCTC AGGTCTATGG TCCAGACACT ACACAGAAGG ATATATTTGA TGGCACAGTA 
AACGGACTTG TCAGAAATGT TCTAGAAGGG CAAAATTCTC TGGTCTTTAC GTATGGTGTC 
ACCAATGCTG GGAAGACCTT TACTTTTTTA GGTCCAGACA CTAATCCGGG CATCTTGCCT 
CGTTCCCTCA ACATGATCTT CAGTAGTCTG GAGGGTCGCA TCTTTACCCA GATGTGCCTG 
AAACCCCAGA GGTGCCGAGA TTTCATCAGA CTTACAAAAG AGCAACAAAA TGAGGAAGCA 
GCCAGCAAAC GCAACCTGCT CAAGCTTTTC AAAGAGTCTG ATGCTCAGAG GAGTATTTCT 
GGCCATTGCT CAAAGTCTGA TATTGAAGAT GGTTCCATGC TCTCTGACGT AAACTCTGCG 
GTTTTGGATG TGGAGATGGA CATGCACACT AGGTTTTCTA TTTGGGTCTC GTTCTGTGAG 
ATCTACAATG AGAACATCCA TGACTTGCTA GAACAGCATG CAAGCAATGC TTCACGGAAA 
ACCAACCTTC GCCTTTGTCA GGATGTCAAA GGAAACTCAT TCATTAAAGA TCTAAAATGG 
GTCCAGGTGA GCAGTGCGGA GGAAGCCCTG AAAGTGATGA AACTGGGCAA AAGAAATCAG 
AGCATCTCTA GCACTAAACT CAACCTGCTC TCCAGCAGAA GCCACAGTAT TTTCTCTGTT 
CGAATACTGA AGGTGGAGGA TGTGGGAATA CCTAGAGTGG AGTCAGTCAG CGAGCTGGCA 
CTGTGTGATC TGGCTGGATC AGAGCGTTGT GCTAAGACTC AAAATAAAGG TGAACGGCTA 
AAAGAGGCTG GCAACATCAA TACTTCTCTG CTCAGTCTTG GAAAGTGCAT TAATGCACTA 
AGGAATAACC AACAGGCTAG ACAGCATGTT CCGTTCAGAG AGAGTAAACT CACTCACTAT 
CTGCAGGGCT ACTTCACAGG CCGGGGCTCA GCCTGTATGA TTGTAAACAT TAATCAGTGC 
TCCTCCATGT ATGATGAGAC GCTGAATGTG CTCAAGTTCT CTGCCGTGGC TCAGAAGGTG 
GTGGTCCTCA CCACTAAGAA CATCCCAGTG GGAGTGAAAA GGAGTGCAAG AGAGGTGTCC 
TTCATAATCA ACAACGCTGA TTGCCGGGAA ATGAGACGCA GCTCATTAGT ACGATGGGAC 
CCAAGTCTTG AGGATGTCCA GGAAGATTCT GACTCAGAGG ACGAGTATGA AGACCAGAGC 
GAAATCGAGG ACACCTTACT AAATTCTGAA GATGAATGTA CAGAGACTGT GCAAATCAAT 
AAAAAGCTTT ATGAGGGCCA GCAGCGGTTA ATAGTGAATA CTCAGCAGCA GTTGACAGCA 
GTACAAGCCG AACTCTTAAC CCTAGAAGCA CGTGTTCGAG ATGAAGTGAC AAAAGAATTC 
TCAGAACTGA TTTCCCAGAT GCAGGAAGAT TACAGTGAAC GTATGTCCAG AGAGCGGGAG 
CTGATAGAGG AACGGTGTGA ACGGAGACTG GAGATTCTCA AAAACCTAGT GGAAAAAACG 
GCTATGGAGG ATGACCGTAT GAATTCAACT GTGGCCAAAT CAGAGGAGGA GAAACGTGTC 
TCTCTAGACA GCCTGTTTGA CTCTATGAGT GGTGACCTGG CAGGAATCAG ACATGATGCC 
CAGGCTGCCC AGTCCTGCTT GGTGACCTCT GATCCCAGAG TCTCAGCGAC ATTGCATGAC 
CTTGAGAAAA AGGTCACAGA ACTTTCAGAA CAGCTCAGCA TATCTCAGAA TCAGCTGTTG 
CTCAAAACAA AGGAGCTTGA AACCCAGAGC CGGCTGTCAG ATGAACTGGA GGAGGTGAAG 
AAGACTCTGG AGAGCCACAG GGAAAGAGAA TTTAATCTGA GTGACATGTG CAGACAGAAG 
GATGAGATGA TTGCCAAACT CCAGGCTACT TTGGACCAGC ATGTAGAAAG AAAGGCTGAG 
GATAGAGCTC AAATGGACTC TGTCCAAAAG GAGGTCATCC AGTGCTGTTG CACCATTAAA 
GAGCCCCTCA GCTCCCGGAA TGAAACCAGA AAGCGACGTC AGGAAGGAGA GGGGCTTGAA 
GGACAGCCCC CGCTCAAGAA AGGCCCCCCG GAGGACTTGA AAATGCCTGA AGATGAAGCA 
GATGATGGGC GGGAAGACTC CGCCCTTCAT GAGGTCAAAG GTCAGCTGGA GTGTTGCCTT 
GCTGAAGCCA AACAAAAAGA CAAGCAGATC ACAGACCTTG AGCAGGAATG CCACAGATTA 
CAAGTGTGCA TTCAGGATCA AAAGTCAAAT CTGGATGAAA GAGTGTCACA GGAACATATT 
CTGAAAGAAG ACGTTTGTCA GACAACATTG GAAAGCAAAC AGATGGCAAC GGCTGTGGAT 
TTTAGCAATC TGAACGTAGC AGTTGCATCC AGATGTAGCA ACACTACAGG TGCGTTTGCT 
GCTAAATCAC TAATGGCTAA GCGAGATCTT GAACTGAGAG AAAAGAACAA ACACATCTTG 
ACTCTTGAAA AGGAAATTGC GCTAATGAGA CAGAAAGCAG AACACACATC TGACTATGAA 
AATCTCAAAA AACTCAACTC TGAACTGCAA GCTGAGGTCA CGAGCTTATG TAAGGTCAAA 
GCCGAACTTG AGGAGAAGAT TACATGCCTT CAGAAGGAGA AGGAGGGGCT TGAAGATAAG 
GAGGTGGGGC TGACAAACCC TAACACTCTT GGATTTAAAA GTAAAGAGAG AGATAACCAA 
ACACACCTCA TGCTGTCTGG AAAGGAGGCT GAACTTGCAC TGAGAGAGAA TGCTCTAACT 
TTAAAGGAAA CTCAGCTTTC AGCACTACAG AAGAGTCTGA AGCAAACACA GGAGCGTCTG 
GAGGAGGAGG AGATGCAGGC GGTGCAGGAG GCGCGCAGGA AGGAGGTGGA GAGGAGGAGA 
GAGATCCTGG CTGTGGCAGA GGAGGCCATC GCTCAGAAAG ATGCAGAGCT ACAGAAGAGA 
CAAGTAGAGA TCAGCCGGTT AAAAGAGGAG ATTAAAACTA GCGAGTTTAA GATGAGCAGT 
CTGACTGTTG ATCTGCGCAG AAGGGAAGAT GATTCATCTG ATCTTCGGGA AAAACTTTGT 
GACTCAAAGA AACAAATACA GCAGGTCCAG AAAGAGATTT CGTCCATGCG TGAAGCTGAG 
AAATCTCTAC GACTGAAGCT CAGCGATCTA GAAAAAACGA AAGTGCAGCT CCAGAATGAG 
ATCGCCAACA GAGATCGCAC CATTAACCAG TTAAAAGCTG AACGATCCTC TGGTTCAAAG 
TCAGATGAGA ATCTGCTGCT CTATCAGAAA GCATGTAAAG ATCTGCAAGA GCGTGAGCGT 
GTTATTGAAG ACATGCGCTT GGCTCTGACA GAACAGGAGG AAACACAGAC ACAGCTGGAC 
CTTGAGTTGG ACAACAGAGA CGCTCGGATT GATGAGCTCA CTCAAGAGCT GGCCAGTTTG 
AGAGAAATGT TGCCTGAAGA GAGGGACAGG AGTGATTCCA GACCAATCTC TAAAGATGTT 
TTACAGGCCA GACAGCAGAT CACTCAGGCC CACGAGAGCT TACAGCTTGC CAATGAGAAG 
CAGCAGGCAG AACGCAAGAA GTGGATGGAG GAGAAGTTGA TTCTGATTGG TCAGGCTAAA 
GAAGCTGAGG AGAGGCGGAA TCAGGACATG AGACGATTTG CAGATGATCG AGAACGGCAT 
GTTCGGCAAC AGGCTGAAAT GGAGTCTCTG GCTGCACGTC TTAATGCCCG TGAGGAAGAG 
ATGGAAAGGT GGAGAAAGGA GAGAGACACG CTTGTCTCTG CTCTGGAGGT TCAGCTGAAG 
AAACTCATCA TGTCCAATGG AGAAAAAGAC CAACAGATCA AGATGCTGCA GTCCAGTAAC 
ACTGCCCAAA CACCAGAGGA GGTCAGTGAG GACAGCAGGA CCGAGGCACT GCAGGCAGAG 
CTGGCTGTAA AAGAGGCTGA GATCCAGAAA CTGAAGGAGC AGCTCAGCAT CAGTGCCACA 
AACAGCAGCA TTCGCAGAAA CTCTACTACA CAGACGATGG AAGTGGAGTT TTCTGATATG 
AAGCCTCTGA ACAGCAGTAC AATCAAGCAC AAAAGCCGAA CCAGAGGATC TGTCATCAGT 
CAGAACTCAT CCGGTAGCGG TCCACTGGTG CTAGATTCAT CAGAGATCTC CACGGAAACA 
GGCAGACGGT CTAGGTTTCC AAGGCCTGAG CTGGAGATTT CCTTCAGTCC TCTACAGCCC 
GATCGCTTCG CTCTCAAGCG TCAGGGTGAA GAATCTGCTG TCACTGTTAA AATCTCCAGA 
CCCACACGCA AGAGGAAGAG CGGCGAGATG GACAAGGATG CGGTGGAAGG TGAAAACATC 
AGAAATACAA GGTGCAGATT GACTCCTCAC CTAACACCTC ATCGGGAAGA GTCCCCAAAT 
TCCCAGGAGT CAAACCAAAG AGGGAAAAAA GACAAAACGC TCCAGAAGAT TGGAGATTTT 
CTTCAGAGCT CTCCCACCTT TCTTGGGAGT AAAGCGAAGA GGATCATGGG ATTGATGAGT 
GGAAAATCCC CTGATGGTGG GTCGACTCCT TCTCTAAAAC TGAAATCCAA ACGTGATAAA 
CGGAAATATG ACAGACCCGA TATCTCCTCA CCAATGGACA TACCGGCTCA TCGGATTATT 
GGCCGAAATC CAGAGGACAA AGAGAGTGAA AACCTTATCA AGAAACGTCT TCGTACAAGA 
ATGGCCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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kif20ba cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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