clpxa cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	clpxa
Entrez Gene ID	447842
Full Name	caseinolytic mitochondrial matrix peptidase chaperone subunit a
Synonyms	clpx,fb93b07,wu:fb93b07,zgc:92303
General protein information	Preferred Names caseinolytic mitochondrial matrix peptidase chaperone subunit a Names ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial ClpX caseinolytic peptidase X homolog a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001004581.1	NP_001004581.1	ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial
XM_021477471.1	XP_021333146.1	ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CLPX	NP_006651.2
Canis lupus familiaris (dog)	CLPX	XP_852508.1
Gallus gallus (chicken)	CLPX	NP_001025723.1
Danio rerio (zebrafish)	clpxa	NP_001004581.1
Drosophila melanogaster (fruit fly)	CG4538	NP_650843.1
Mus musculus (house mouse)	Clpx	NP_035932.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002996	XP_003436341.1
Neurospora crassa	NCU05427	XP_963250.2
Bos taurus (cattle)	CLPX	XP_002690567.1
Rattus norvegicus (Norway rat)	Clpx	NP_001007804.1
Pan troglodytes (chimpanzee)	CLPX	XP_001174382.1
	CLPX	XP_001108768.1
Kluyveromyces lactis	KLLA0F09691g	XP_455520.1
Caenorhabditis elegans (roundworm)	D2030.2	NP_001021076.1
Saccharomyces cerevisiae (baker's yeast)	MCX1	NP_009786.1
Magnaporthe oryzae (rice blast fungus)	MGG_03187	XP_003716802.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutation in human
Yien YY, Ducamp S, van der Vorm LN, Kardon JR, Manceau H, Kannengiesser C, Bergonia HA, Kafina MD, Karim Z, Gouya L, Baker TA, Puy H, Phillips JD, Nicolas G, Paw BH
Proceedings of the National Academy of Sciences of the United States of America114(38)E8045-E8052(2017 Sep)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Mitochondrial ClpX Activates a Key Enzyme for Heme Biosynthesis and Erythropoiesis.
Kardon JR, Yien YY, Huston NC, Branco DS, Hildick-Smith GJ, Rhee KY, Paw BH, Baker TA
Cell161(4)858-67(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following clpxa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the clpxa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03328	NM_001004581.1 Latest version!	Danio rerio caseinolytic mitochondrial matrix peptidase chaperone subunit Xa (clpxa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa143241	XM_021477471.1 Latest version!	Danio rerio caseinolytic mitochondrial matrix peptidase chaperone subunit a (clpxa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

clpxa ( NM_001004581.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001004581.1 Danio rerio caseinolytic mitochondrial matrix peptidase chaperone subunit Xa (clpxa), mRNA.

Price & Availability↑

CloneID	ODa03328
Clone ID Related Accession (Same CDS sequence)	NM_001004581.1
Accession Version	NM_001004581.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Danio rerio(zebrafish)
Product	ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081643.1. ##Evidence-Data-START## Transcript exon combination :: BC081643.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTCGTGCA CGTGTGCTAT AGCAGCGAGA AAATTCATAA ATTCAGCTCA TAAAGGAATA 
TCCGGCTCCA GGGTCCAGTT ACTGTCACTG AGCCGTCCAG GGACCCGTGA GGTTCGTCTG 
GCACGCCGCG TCCCTGTGAG ATCCTTCTCA GAGACGGCTG TGTATTATGC ATCAAAGGAT 
GGAGTAAAAG ATGGAGATGG TGGGAAGAAA TCTGTTGGTG AAGGAAGTGG AAAAAGAACC 
AGCTCTGGTA ACTCTGGCAA AGGAGGAAGT CAGCTGAGGT GCCCCAAATG CGGAGATCCC 
TGCACACATG TAGAAACATT TGTTTCATCA ACACGGTTTG TGAAATGTGA GAAATGCCAC 
CACTTCTTCG TCGTGCTGTC AGAGACGGAC ACTAAAAAGA GTCTGAGTAA AGACCCGGAG 
TCTGCGGCAG AAGCTGTCAA GTTGGCTTTC CAACAGAAAC CTCCTCCACC ACCTAAAAAG 
ATCTTTGCTT ATCTTGATAA GTATGTTGTG GGTCAGGACC ATGCTAAGAA GGTATTGTCA 
GTGGCAGTGT ACAATCATTA TAAACGCATC TACAACAACA TGCCGGCGGG CTCCAGGCAG 
CAGCAGGTGG AGGTGGAGAA ACAGGCCTCG CTCACTCCTC GAGAGCTGCT TCAGATCGCA 
GGGATCAGTC CTCATGGAAA TGCACTGGGC GCCTCCATGC AGCAGCAACT GAACCAGCAG 
ACTCCTCCAG AGAAGAGAGG AGGAGAAGTG CTGGACTCCA CACACACCGA CATAAAGCTG 
GAGAAGAGCA ACATCGTCCT GCTCGGACCC ACCGGCTCTG GTAAAACACT TCTGGCTCAA 
ACGCTGGCCA AATGCCTGGA TGTCCCCTTT GCAATCTGTG ACTGCACCAC GCTGACCCAG 
GCAGGGTATG TGGGAGAAGA CATCGAGTCA GTCATCGCAA AACTACTGCA GGACGCCAAC 
TATGTGATCG AGAAAGCTCA GCAAGGTATC GTGTTCCTGG ACGAAGTGGA CAAGATCGGC 
AGTGTTCCCG GAATCCATCA GTTAAGGGAC GTGGGAGGAG AGGGAGTGCA ACAGGGGTTA 
CTGAAGCTGC TGGAAGGCAC TATTGTTAAT GTTCCAGAGA AGAACACCAG GAAGCTGAGA 
GGAGAAACGG TGCAGGTCGA CACCACCAAT ATCCTGTTTG TAGCATCTGG AGCTTTTAAT 
GGACTTGACC GCATCATCAG CAGGAGAAAG AACGAGAAGT ATCTTGGGTT TGGCACACCG 
TCTAACATGG GGAAAGGCAG AAGAGCGGCG GCAGCAGCTG ATCTGGCCAA CACCACCGGT 
GGAGAAGTGG ATGCTGTGGC CGAGATCGAG GAGAAGGACC GGCTTCTGAA ACATGTTGAG 
GCCCGAGACC TTATTGAGTT CGGAATGATC CCTGAGTTTG TGGGTCGTCT CCCTGTTGTG 
GTTCCTCTGC ACAGTCTGGA TGAGGAGACG CTGGTGCGGA TCCTCACAGA GCCACGCAAT 
GCTGTCGTGC CTCAGTATCA GGCTCTCTTC AGCATGGACA AGTGTGAGCT CAACATGACA 
CCTGATGCTC TGAGAGCGAT TGCCAGATTG GCTTTGGAGC GTAAAACTGG AGCACGTGGA 
CTCAGATCCA TAATGGAAAA ACTGCTGCTA GACCCCATGT TCGAGGTGCC ACATTCAGAC 
ATTGTGTCTG TGGACGTTAG CAAGGACGTT GTGCAAGGAA AAGCGCCGCC TCAGTACATA 
AGAGCTGCAG CTAAAGAGTC CTCTGAGGAA GAGTACGACT CAGGAATCGA AGAGGAAAAC 
TGGACAAGAC AAGCCGATGC CGCCAAGAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004581.1
CDS	237..2069
Translation	MSCTCAIAARKFINSAHKGISGSRVQLLSLSRPGTREVRLARRVPVRSFSETAVYYASKDGVKDGDGGKKSVGEGSGKRTSSGNSGKGGSQLRCPKCGDPCTHVETFVSSTRFVKCEKCHHFFVVLSETDTKKSLSKDPESAAEAVKLAFQQKPPPPPKKIFAYLDKYVVGQDHAKKVLSVAVYNHYKRIYNNMPAGSRQQQVEVEKQASLTPRELLQIAGISPHGNALGASMQQQLNQQTPPEKRGGEVLDSTHTDIKLEKSNIVLLGPTGSGKTLLAQTLAKCLDVPFAICDCTTLTQAGYVGEDIESVIAKLLQDANYVIEKAQQGIVFLDEVDKIGSVPGIHQLRDVGGEGVQQGLLKLLEGTIVNVPEKNTRKLRGETVQVDTTNILFVASGAFNGLDRIISRRKNEKYLGFGTPSNMGKGRRAAAAADLANTTGGEVDAVAEIEEKDRLLKHVEARDLIEFGMIPEFVGRLPVVVPLHSLDEETLVRILTEPRNAVVPQYQALFSMDKCELNMTPDALRAIARLALERKTGARGLRSIMEKLLLDPMFEVPHSDIVSVDVSKDVVQGKAPPQYIRAAAKESSEEEYDSGIEEENWTRQADAAKN

Target ORF information:

RefSeq Version	NM_001004581.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio caseinolytic mitochondrial matrix peptidase chaperone subunit Xa (clpxa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004581.1

ORF Insert Sequence:

ATGTCGTGCA CGTGTGCTAT AGCAGCGAGA AAATTCATAA ATTCAGCTCA TAAAGGAATA 
TCCGGCTCCA GGGTCCAGTT ACTGTCACTG AGCCGTCCAG GGACCCGTGA GGTTCGTCTG 
GCACGCCGCG TCCCTGTGAG ATCCTTCTCA GAGACGGCTG TGTATTATGC ATCAAAGGAT 
GGAGTAAAAG ATGGAGATGG TGGGAAGAAA TCTGTTGGTG AAGGAAGTGG AAAAAGAACC 
AGCTCTGGTA ACTCTGGCAA AGGAGGAAGT CAGCTGAGGT GCCCCAAATG CGGAGATCCC 
TGCACACATG TAGAAACATT TGTTTCATCA ACACGGTTTG TGAAATGTGA GAAATGCCAC 
CACTTCTTCG TCGTGCTGTC AGAGACGGAC ACTAAAAAGA GTCTGAGTAA AGACCCGGAG 
TCTGCGGCAG AAGCTGTCAA GTTGGCTTTC CAACAGAAAC CTCCTCCACC ACCTAAAAAG 
ATCTTTGCTT ATCTTGATAA GTATGTTGTG GGTCAGGACC ATGCTAAGAA GGTATTGTCA 
GTGGCAGTGT ACAATCATTA TAAACGCATC TACAACAACA TGCCGGCGGG CTCCAGGCAG 
CAGCAGGTGG AGGTGGAGAA ACAGGCCTCG CTCACTCCTC GAGAGCTGCT TCAGATCGCA 
GGGATCAGTC CTCATGGAAA TGCACTGGGC GCCTCCATGC AGCAGCAACT GAACCAGCAG 
ACTCCTCCAG AGAAGAGAGG AGGAGAAGTG CTGGACTCCA CACACACCGA CATAAAGCTG 
GAGAAGAGCA ACATCGTCCT GCTCGGACCC ACCGGCTCTG GTAAAACACT TCTGGCTCAA 
ACGCTGGCCA AATGCCTGGA TGTCCCCTTT GCAATCTGTG ACTGCACCAC GCTGACCCAG 
GCAGGGTATG TGGGAGAAGA CATCGAGTCA GTCATCGCAA AACTACTGCA GGACGCCAAC 
TATGTGATCG AGAAAGCTCA GCAAGGTATC GTGTTCCTGG ACGAAGTGGA CAAGATCGGC 
AGTGTTCCCG GAATCCATCA GTTAAGGGAC GTGGGAGGAG AGGGAGTGCA ACAGGGGTTA 
CTGAAGCTGC TGGAAGGCAC TATTGTTAAT GTTCCAGAGA AGAACACCAG GAAGCTGAGA 
GGAGAAACGG TGCAGGTCGA CACCACCAAT ATCCTGTTTG TAGCATCTGG AGCTTTTAAT 
GGACTTGACC GCATCATCAG CAGGAGAAAG AACGAGAAGT ATCTTGGGTT TGGCACACCG 
TCTAACATGG GGAAAGGCAG AAGAGCGGCG GCAGCAGCTG ATCTGGCCAA CACCACCGGT 
GGAGAAGTGG ATGCTGTGGC CGAGATCGAG GAGAAGGACC GGCTTCTGAA ACATGTTGAG 
GCCCGAGACC TTATTGAGTT CGGAATGATC CCTGAGTTTG TGGGTCGTCT CCCTGTTGTG 
GTTCCTCTGC ACAGTCTGGA TGAGGAGACG CTGGTGCGGA TCCTCACAGA GCCACGCAAT 
GCTGTCGTGC CTCAGTATCA GGCTCTCTTC AGCATGGACA AGTGTGAGCT CAACATGACA 
CCTGATGCTC TGAGAGCGAT TGCCAGATTG GCTTTGGAGC GTAAAACTGG AGCACGTGGA 
CTCAGATCCA TAATGGAAAA ACTGCTGCTA GACCCCATGT TCGAGGTGCC ACATTCAGAC 
ATTGTGTCTG TGGACGTTAG CAAGGACGTT GTGCAAGGAA AAGCGCCGCC TCAGTACATA 
AGAGCTGCAG CTAAAGAGTC CTCTGAGGAA GAGTACGACT CAGGAATCGA AGAGGAAAAC 
TGGACAAGAC AAGCCGATGC CGCCAAGAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

clpxa ( XM_021477471.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333146.1 Danio rerio caseinolytic mitochondrial matrix peptidase chaperone subunit a (clpxa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa143241
Clone ID Related Accession (Same CDS sequence)	XM_021477471.1
Accession Version	XM_021477471.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTGCA CGTGTGCTAT AGCAGCGAGA AAATTCATAA ATTCAGCTCA TAAAGGAATA 
TCCGGCTCCA GGGTCCAGTT ACTGTCACTG AGCCGTCCAG GGACCCGTGA GGTTCGTCTG 
GCACGCCGCG TCCCTGTGAG ATCCTTCTCA GAGACGGCTG TGTATTATGC ATCAAAGGAT 
GGAGTAAAAG ATGGAGATGG TGGGAAGAAA TCTGTTGGTG AAGGAAGTGG AAAAAGAACC 
AGCTCTGGTA ACTCTGGCAA AGGAGGAAGT CAGCTGAGGT GCCCCAAATG CGGAGATCCC 
TGCACACATG TAGAAACATT TGTTTCATCA ACACGGTTTG TGAAATGTGA GAAATGCCAC 
CACTTCTTCG TCGTGCTGTC AGAGACGGAC ACTAAAAAGA GTCTGAGTAA AGACCCGGAG 
TCTGCGGCAG AAGCTGTCAA GTTGGCTTTC CAACAGAAAC CTCCTCCACC ACCTAAAAAG 
ATCTTTGCTT ATCTTGATAA GTATGTTGTG GGTCAGGACC ATGCTAAGAA GGTATTGTCA 
GTGGCAGTGT ACAATCATTA TAAACGCATC TACAACAACA TGCCGGCGGG CTCCAGGCAG 
CAGCAGGTGG AGGTGGAGAA ACAGGCCTCG CTCACTCCTC GAGAGCTAGA GCTAAGACGG 
CGAGAGGACG AATACAGGTT TACAAAGCTG CTTCAGATCG CAGGGATCAG TCCTCATGGA 
AATGCACTGG GCGCCTCCAT GCAGCAGCAG CTGAACCAGC AGACTCCTCC CGAGAAGAGA 
GGAGGAGAAG TGCTGGACTC CACACACACC GACATAAAGC TGGAGAAGAG CAACATCGTC 
CTGCTCGGAC CCACTGGCTC TGGTAAAACA CTTCTGGCTC AAACGCTGGC CAAATGCCTG 
GATGTCCCCT TTGCAATCTG TGACTGCACC ACGCTGACCC AGGCAGGGTA TGTGGGAGAA 
GACATCGAGT CAGTCATCGC AAAACTACTG CAGGACGCCA ACTATGTGAT CGAGAAAGCT 
CAGCAAGGTA TCGTGTTCCT GGACGAAGTG GACAAGATCG GCAGTGTTCC CGGAATCCAT 
CAGTTAAGGG ACGTGGGAGG AGAGGGAGTG CAGCAGGGGT TACTGAAGCT GCTGGAAGGC 
ACTATTGTTA ATGTTCCAGA GAAGAACACC AGGAAGCTGA GAGGAGAAAC GGTGCAGGTC 
GACACCACCA ATATCCTGTT TGTAGCATCT GGAGCTTTTA ATGGACTTGA CCGCATCATC 
AGCAGGAGAA AGAACGAGAA GTATCTTGGG TTTGGTACAC CGTCTAACAT GGGGAAAGGC 
AGAAGAGCGG CGGCAGCAGC TGATCTGGCC AACACCACCG GTGGAGAAGT GGATGCTGTG 
GCCGAGATCG AGGAGAAGGA CCGGCTTCTG AAACATGTGG AGGCCCGAGA CCTTATTGAG 
TTCGGAATGA TCCCTGAGTT TGTGGGTCGT CTCCCTGTTG TGGTTCCTCT GCACAGTCTG 
GATGAGGAGA CGCTGGTGCG GATCCTCACA GAGCCACGCA ATGCTGTCGT GCCTCAGTAT 
CAGGCTCTCT TCAGCATGGA CAAGTGTGAG CTCAACATGA CACCTGATGC TCTGAGAGCG 
ATTGCCAGAT TGGCTTTGGA GCGTAAAACT GGAGCACGTG GACTCAGATC CATAATGGAA 
AAACTGCTGC TAGACCCCAT GTTCGAGGTG CCACATTCAG ACATTGTGTC TGTGGACGTT 
AGCAAGGACG TTGTGCAAGG AAAAGCGCCG CCTCAGTACA TAAGAGCTGC AGCTAAAGAG 
TCCTCTGAGG AAGAGTACGA CTCAGGAATC GAAGAGGAAA ACTGGACAAG ACAAGCCGAT 
GCCGCCAAGA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333146.1
CDS	305..2179
Translation	MSCTCAIAARKFINSAHKGISGSRVQLLSLSRPGTREVRLARRVPVRSFSETAVYYASKDGVKDGDGGKKSVGEGSGKRTSSGNSGKGGSQLRCPKCGDPCTHVETFVSSTRFVKCEKCHHFFVVLSETDTKKSLSKDPESAAEAVKLAFQQKPPPPPKKIFAYLDKYVVGQDHAKKVLSVAVYNHYKRIYNNMPAGSRQQQVEVEKQASLTPRELELRRREDEYRFTKLLQIAGISPHGNALGASMQQQLNQQTPPEKRGGEVLDSTHTDIKLEKSNIVLLGPTGSGKTLLAQTLAKCLDVPFAICDCTTLTQAGYVGEDIESVIAKLLQDANYVIEKAQQGIVFLDEVDKIGSVPGIHQLRDVGGEGVQQGLLKLLEGTIVNVPEKNTRKLRGETVQVDTTNILFVASGAFNGLDRIISRRKNEKYLGFGTPSNMGKGRRAAAAADLANTTGGEVDAVAEIEEKDRLLKHVEARDLIEFGMIPEFVGRLPVVVPLHSLDEETLVRILTEPRNAVVPQYQALFSMDKCELNMTPDALRAIARLALERKTGARGLRSIMEKLLLDPMFEVPHSDIVSVDVSKDVVQGKAPPQYIRAAAKESSEEEYDSGIEEENWTRQADAAKN

Target ORF information:

RefSeq Version	XM_021477471.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio caseinolytic mitochondrial matrix peptidase chaperone subunit a (clpxa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477471.1

ORF Insert Sequence:

ATGTCGTGCA CGTGTGCTAT AGCAGCGAGA AAATTCATAA ATTCAGCTCA TAAAGGAATA 
TCCGGCTCCA GGGTCCAGTT ACTGTCACTG AGCCGTCCAG GGACCCGTGA GGTTCGTCTG 
GCACGCCGCG TCCCTGTGAG ATCCTTCTCA GAGACGGCTG TGTATTATGC ATCAAAGGAT 
GGAGTAAAAG ATGGAGATGG TGGGAAGAAA TCTGTTGGTG AAGGAAGTGG AAAAAGAACC 
AGCTCTGGTA ACTCTGGCAA AGGAGGAAGT CAGCTGAGGT GCCCCAAATG CGGAGATCCC 
TGCACACATG TAGAAACATT TGTTTCATCA ACACGGTTTG TGAAATGTGA GAAATGCCAC 
CACTTCTTCG TCGTGCTGTC AGAGACGGAC ACTAAAAAGA GTCTGAGTAA AGACCCGGAG 
TCTGCGGCAG AAGCTGTCAA GTTGGCTTTC CAACAGAAAC CTCCTCCACC ACCTAAAAAG 
ATCTTTGCTT ATCTTGATAA GTATGTTGTG GGTCAGGACC ATGCTAAGAA GGTATTGTCA 
GTGGCAGTGT ACAATCATTA TAAACGCATC TACAACAACA TGCCGGCGGG CTCCAGGCAG 
CAGCAGGTGG AGGTGGAGAA ACAGGCCTCG CTCACTCCTC GAGAGCTAGA GCTAAGACGG 
CGAGAGGACG AATACAGGTT TACAAAGCTG CTTCAGATCG CAGGGATCAG TCCTCATGGA 
AATGCACTGG GCGCCTCCAT GCAGCAGCAG CTGAACCAGC AGACTCCTCC CGAGAAGAGA 
GGAGGAGAAG TGCTGGACTC CACACACACC GACATAAAGC TGGAGAAGAG CAACATCGTC 
CTGCTCGGAC CCACTGGCTC TGGTAAAACA CTTCTGGCTC AAACGCTGGC CAAATGCCTG 
GATGTCCCCT TTGCAATCTG TGACTGCACC ACGCTGACCC AGGCAGGGTA TGTGGGAGAA 
GACATCGAGT CAGTCATCGC AAAACTACTG CAGGACGCCA ACTATGTGAT CGAGAAAGCT 
CAGCAAGGTA TCGTGTTCCT GGACGAAGTG GACAAGATCG GCAGTGTTCC CGGAATCCAT 
CAGTTAAGGG ACGTGGGAGG AGAGGGAGTG CAGCAGGGGT TACTGAAGCT GCTGGAAGGC 
ACTATTGTTA ATGTTCCAGA GAAGAACACC AGGAAGCTGA GAGGAGAAAC GGTGCAGGTC 
GACACCACCA ATATCCTGTT TGTAGCATCT GGAGCTTTTA ATGGACTTGA CCGCATCATC 
AGCAGGAGAA AGAACGAGAA GTATCTTGGG TTTGGTACAC CGTCTAACAT GGGGAAAGGC 
AGAAGAGCGG CGGCAGCAGC TGATCTGGCC AACACCACCG GTGGAGAAGT GGATGCTGTG 
GCCGAGATCG AGGAGAAGGA CCGGCTTCTG AAACATGTGG AGGCCCGAGA CCTTATTGAG 
TTCGGAATGA TCCCTGAGTT TGTGGGTCGT CTCCCTGTTG TGGTTCCTCT GCACAGTCTG 
GATGAGGAGA CGCTGGTGCG GATCCTCACA GAGCCACGCA ATGCTGTCGT GCCTCAGTAT 
CAGGCTCTCT TCAGCATGGA CAAGTGTGAG CTCAACATGA CACCTGATGC TCTGAGAGCG 
ATTGCCAGAT TGGCTTTGGA GCGTAAAACT GGAGCACGTG GACTCAGATC CATAATGGAA 
AAACTGCTGC TAGACCCCAT GTTCGAGGTG CCACATTCAG ACATTGTGTC TGTGGACGTT 
AGCAAGGACG TTGTGCAAGG AAAAGCGCCG CCTCAGTACA TAAGAGCTGC AGCTAAAGAG 
TCCTCTGAGG AAGAGTACGA CTCAGGAATC GAAGAGGAAA ACTGGACAAG ACAAGCCGAT 
GCCGCCAAGA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutation in human
Proceedings of the National Academy of Sciences of the United States of America114(38)E8045-E8052(2017 Sep)
Yien YY,Ducamp S,van der Vorm LN,Kardon JR,Manceau H,Kannengiesser C,Bergonia HA,Kafina MD,Karim Z,Gouya L,Baker TA,Puy H,Phillips JD,Nicolas G,Paw BH

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Mitochondrial ClpX Activates a Key Enzyme for Heme Biosynthesis and Erythropoiesis.
Cell161(4)858-67(2015 May)
Kardon JR,Yien YY,Huston NC,Branco DS,Hildick-Smith GJ,Rhee KY,Paw BH,Baker TA

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clpxa cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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