pkmb cDNA ORF clone, Danio rerio(zebrafish)

The following pkmb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pkmb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa01489 NM_001003488.1
Latest version!
Danio rerio pyruvate kinase M1/2b (pkmb), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ODa57796 XM_005163113.4
Latest version!
Danio rerio pyruvate kinase, muscle, b (pkmb), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
View Old Accession Versions >>
ODa57796 XM_005163113.3 Danio rerio pyruvate kinase, muscle, b (pkmb), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa01489
    Clone ID Related Accession (Same CDS sequence) NM_001003488.1
    Accession Version NM_001003488.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1593bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-02
    Organism Danio rerio(zebrafish)
    Product pyruvate kinase isozymes M1/M2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC076497.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC076497.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505371 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCTCAGA CAAAGACTAC GGCCTCTGGC TTCATTCAGA CACAGCAGAT GCCTGCTGCA 
    ATGGCGGAAA CCTTCTTGGA GCATCTGTGC CTGCTGGACA TCGACTCTGA ACCTACAGTG
    TCTCGAAACA CCGGCATCGT CTGCACACTC GGACCTGCTT CACGTTCTTT GGAGACACTG
    AGAGAGATGA TCTTGTCCGG CATGAACGTC GCACGCCTGA ACTTCTCTCA CGGAACTCAT
    GAGTACCATG CTGAAACCAT CAAGAGTGTC CGTGAAGCTA TTGAGAGTTT TGGAGCTGGT
    ACCATTGATT ACCGACCTGT TGCCATTGCC CTGGATACCA AGGGCCCTGA GATCAGAACT
    GGGCTTATTA AGGGCAGTGG CACTGAGGAG GTCAAGCTTG TCAAAGGTAA CATAATCAAA
    CTGACTTTGG ATGACAAGTT CATGGACAAC TGTGATGAAA ATACCCTGTG GTTGGACTAC
    AAGAACATCA CAAAGGTGGT GCAGCAGGGC AGCCATATTT ACGTGGACGA TGGTCTGATT
    TCACTCAAGG TTAAGGAAAT CGGATCTGAC TTCCTGAACT GTGAGATTGA GAACGGAGGC
    ATGTTGGGCA GCAAGAAAGG TGTGAACCTG CCTGGAGCCA ATGTTGACCT GCCCGCAGTG
    TCTGAGAAGG ACATCAAGGA CCTTCAGTTT GGTGTGGAAC AGGGCGTGGA CATGGTTTTT
    GCCTCCTTTA TCCGCAAAGC TGCTGATGTG CACGCTGTCA GGAAGGTGCT CGGAGAGAAA
    GGGAAGGATA TCAGGATCAT CAGTAAGCTG GAGAACCATG AGGGTGTGCG CAAGTTTGAT
    GAGATCCTTG AGGCCAGTGA TGGTATCATG GTCGCCCGTG GAGATCTGGG TATTGAAATC
    CCTACTGAAA AGGTGTTCCT AGCTCAGAAG ATGATGATTA GCCGTTGCAA TAGGATTGGC
    AAACCAATCA TCTGTGCCAC ACAGATGCTG GAGAGCATGA TAAAGAAGCC TCGTCCTACC
    CGTGCCGAGA GCAGTGATGT GGCCAATGCT GTGCTCGACG GTGCCGACTG CATCATGCTG
    AGCGGAGAGA CGGCAAAAGG AGAATACCCA ATAGAGTCTG TGCTCACACA GCACCTGATC
    GCCCGTGAAG CCGAAGCCGC CATGTTCCAC AGGCAGTTGT TCGAAGAGCT TCGTCGTACC
    TCCCATCTGA CCCGTGATCC CACTGAGTCT GTGGCCATTG GCGCAGTGGA AGCCTCCTTC
    AAGTGCTGTG CCAGCGCCAT TATTTGCCTC ACCAAGACTG GCAGGTCTGC CCAGCTGCTG
    TCCAGATACA GGCCACGTGC TCCTATCATG GCTGTGACCC GTAACGGTCA GACATCTCGG
    CAGCTGCACC TGTATCGTGG CGTCATCCCC ATCCTCTACA CCAAGCCTGC CAATGACATA
    TGGGCTGAGG ATGTGGATCT CCGTGTCAGC TTCGCACTGG AGATCGGCAA GCATCGGAAG
    TACTTCAAGT CCGGTGATGT TATCATCGTC GTCACAGGCT GGCGTCCAGG ATCTGGATAC
    ACCAACACCA TGCGCATCGT TGTGGTGCCT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001003488.1
    CDS25..1617
    Translation

    Target ORF information:

    RefSeq Version NM_001003488.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pyruvate kinase M1/2b (pkmb), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001003488.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCTCAGA CAAAGACTAC GGCCTCTGGC TTCATTCAGA CACAGCAGAT GCCTGCTGCA 
    ATGGCGGAAA CCTTCTTGGA GCATCTGTGC CTGCTGGACA TCGACTCTGA ACCTACAGTG
    TCTCGAAACA CCGGCATCGT CTGCACACTC GGACCTGCTT CACGTTCTTT GGAGACACTG
    AGAGAGATGA TCTTGTCCGG CATGAACGTC GCACGCCTGA ACTTCTCTCA CGGAACTCAT
    GAGTACCATG CTGAAACCAT CAAGAGTGTC CGTGAAGCTA TTGAGAGTTT TGGAGCTGGT
    ACCATTGATT ACCGACCTGT TGCCATTGCC CTGGATACCA AGGGCCCTGA GATCAGAACT
    GGGCTTATTA AGGGCAGTGG CACTGAGGAG GTCAAGCTTG TCAAAGGTAA CATAATCAAA
    CTGACTTTGG ATGACAAGTT CATGGACAAC TGTGATGAAA ATACCCTGTG GTTGGACTAC
    AAGAACATCA CAAAGGTGGT GCAGCAGGGC AGCCATATTT ACGTGGACGA TGGTCTGATT
    TCACTCAAGG TTAAGGAAAT CGGATCTGAC TTCCTGAACT GTGAGATTGA GAACGGAGGC
    ATGTTGGGCA GCAAGAAAGG TGTGAACCTG CCTGGAGCCA ATGTTGACCT GCCCGCAGTG
    TCTGAGAAGG ACATCAAGGA CCTTCAGTTT GGTGTGGAAC AGGGCGTGGA CATGGTTTTT
    GCCTCCTTTA TCCGCAAAGC TGCTGATGTG CACGCTGTCA GGAAGGTGCT CGGAGAGAAA
    GGGAAGGATA TCAGGATCAT CAGTAAGCTG GAGAACCATG AGGGTGTGCG CAAGTTTGAT
    GAGATCCTTG AGGCCAGTGA TGGTATCATG GTCGCCCGTG GAGATCTGGG TATTGAAATC
    CCTACTGAAA AGGTGTTCCT AGCTCAGAAG ATGATGATTA GCCGTTGCAA TAGGATTGGC
    AAACCAATCA TCTGTGCCAC ACAGATGCTG GAGAGCATGA TAAAGAAGCC TCGTCCTACC
    CGTGCCGAGA GCAGTGATGT GGCCAATGCT GTGCTCGACG GTGCCGACTG CATCATGCTG
    AGCGGAGAGA CGGCAAAAGG AGAATACCCA ATAGAGTCTG TGCTCACACA GCACCTGATC
    GCCCGTGAAG CCGAAGCCGC CATGTTCCAC AGGCAGTTGT TCGAAGAGCT TCGTCGTACC
    TCCCATCTGA CCCGTGATCC CACTGAGTCT GTGGCCATTG GCGCAGTGGA AGCCTCCTTC
    AAGTGCTGTG CCAGCGCCAT TATTTGCCTC ACCAAGACTG GCAGGTCTGC CCAGCTGCTG
    TCCAGATACA GGCCACGTGC TCCTATCATG GCTGTGACCC GTAACGGTCA GACATCTCGG
    CAGCTGCACC TGTATCGTGG CGTCATCCCC ATCCTCTACA CCAAGCCTGC CAATGACATA
    TGGGCTGAGG ATGTGGATCT CCGTGTCAGC TTCGCACTGG AGATCGGCAA GCATCGGAAG
    TACTTCAAGT CCGGTGATGT TATCATCGTC GTCACAGGCT GGCGTCCAGG ATCTGGATAC
    ACCAACACCA TGCGCATCGT TGTGGTGCCT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa57796
    Clone ID Related Accession (Same CDS sequence) XM_005163113.3 , XM_005163113.4
    Accession Version XM_005163113.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1818bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pyruvate kinase isozymes M1/M2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163113.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTCATTAT ACTCGATAAA TCGTGTTATA AATGTATTTC TACACAATAC ATGCAAAATG 
    TTCAACTGTG AAGTCAACCC AGTCCTGGCG GAAGAACGCA TTGCTGTGGG AAGTAGTTCC
    GGTCGGTATA GAGTGGATAA GTTAAGGCAG TGGCGGATGA AGAGCTCAGA AGTTCCTGCA
    GGCGTTCATC AACAGAGGAC TCTTTTAGCA GACGCAGCTC CAGCCATGTC TCAGACAAAG
    ACTACGGCCT CTGGCTTCAT TCAGACACAG CAGATGCCTG CTGCAATGGC GGAAACCTTC
    TTGGAGCATC TTTGCCTGCT GGACATCGAC TCTGAACCTA CAGTGTCTCG AAACACTGGC
    ATCGTCTGCA CACTCGGACC TGCTTCACGT TCTTTGGAGA CACTGAGAGA GATGATCTTG
    TCCGGCATGA ACGTCGCACG CCTGAACTTC TCTCACGGAA CTCACGAGTA CCATGCTGAA
    ACCATCAAGA GTGTCCGTGA AGCTATTGAG AGTTTTGGAG CTGGTACCAT TGATTACCGA
    CCTGTTGCCA TTGCCCTGGA TACCAAGGGC CCTGAGATCA GAACTGGGCT TATTAAGGGC
    AGTGGCACTG AGGAGGTCAA GCTTGTCAAA GGTAACATAA TCAAACTGAC TTTGGATGAC
    AAGTTCATGG ACAACTGTGA TGAAAATACC CTGTGGTTGG ACTACAAGAA CATCACAAAG
    GTGGTGCAGC AGGGCAGCCA TATTTACGTG GACGATGGTC TGATTTCACT CAAGGTTAAG
    GAAATCGGAT CTGACTTCCT GAACTGTGAG ATTGAGAACG GAGGCATGTT GGGCAGCAAG
    AAAGGTGTGA ACCTGCCTGG AGCCAATGTT GACCTGCCCG CAGTGTCTGA GAAGGACATC
    AAGGACCTTC AGTTTGGTGT GGAACAGGGC GTGGACATGG TTTTTGCCTC CTTTATCCGC
    AAAGCTGCTG ATGTGCACGC TGTCAGGAAG GTGCTCGGAG AGAAAGGGAA GGATATCAGG
    ATCATCAGTA AGCTGGAGAA CCATGAGGGT GTGCGCAAGT TTGATGAGAT CCTTGAGGCC
    AGTGATGGTA TCATGGTCGC CCGTGGAGAT CTGGGTATTG AAATCCCTAC TGAAAAGGTG
    TTCCTAGCTC AGAAGATGAT GATTAGCCGT TGCAATAGGA TTGGCAAACC AATCATCTGT
    GCCACACAGA TGCTGGAGAG CATGATAAAG AAGCCTCGTC CTACCCGTGC CGAGAGCAGT
    GATGTGGCCA ATGCTGTGCT CGACGGTGCC GACTGCATCA TGCTGAGCGG AGAGACGGCA
    AAAGGAGAAT ACCCAATAGA GTCTGTGCTC ACACAGCACC TGATCGCCCG TGAAGCCGAA
    GCCGCCATGT TCCACAGGCA GTTGTTCGAA GAGCTTCGTC GTACCTCCCA TCTGACCCGT
    GATCCCACTG AGTCTGTGGC CGTTGGCGCA GTGGAAGCCT CCTTCAAGTG CTGTGCCAGC
    GCCATTATTT GCCTCACCAA GACTGGCAGG TCTGCCCAGC TGCTGTCCAG ATACAGGCCT
    CGTGCTCCTA TCATGGCTGT GACCCGTAAC GGTCAGACAT CTCGGCAGCT GCACCTGTAT
    CGTGGCGTCA TCCCCATCCT CTACACCAAG CCTGCCAATG ACATATGGGC TGAGGATGTG
    GATCTCCGTG TCAGCTTCGC ACTGGAGATC GGCAAGCATC GGAAGTACTT CAAGTCCGGT
    GATGTTATCA TCGTCGTCAC AGGCTGGCGT CCAGGATCTG GATACACCAA CACCATGCGC
    ATCGTTGTGG TGCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005163170.3
    CDS307..2124
    Translation

    Target ORF information:

    RefSeq Version XM_005163113.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pyruvate kinase, muscle, b (pkmb), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005163113.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTCATTAT ACTCGATAAA TCGTGTTATA AATGTATTTC TACACAATAC ATGCAAAATG 
    TTCAACTGTG AAGTCAACCC AGTCCTGGCG GAAGAACGCA TTGCTGTGGG AAGTAGTTCC
    GGTCGGTATA GAGTGGATAA GTTAAGGCAG TGGCGGATGA AGAGCTCAGA AGTTCCTGCA
    GGCGTTCATC AACAGAGGAC TCTTTTAGCA GACGCAGCTC CAGCCATGTC TCAGACAAAG
    ACTACGGCCT CTGGCTTCAT TCAGACACAG CAGATGCCTG CTGCAATGGC GGAAACCTTC
    TTGGAGCATC TTTGCCTGCT GGACATCGAC TCTGAACCTA CAGTGTCTCG AAACACTGGC
    ATCGTCTGCA CACTCGGACC TGCTTCACGT TCTTTGGAGA CACTGAGAGA GATGATCTTG
    TCCGGCATGA ACGTCGCACG CCTGAACTTC TCTCACGGAA CTCACGAGTA CCATGCTGAA
    ACCATCAAGA GTGTCCGTGA AGCTATTGAG AGTTTTGGAG CTGGTACCAT TGATTACCGA
    CCTGTTGCCA TTGCCCTGGA TACCAAGGGC CCTGAGATCA GAACTGGGCT TATTAAGGGC
    AGTGGCACTG AGGAGGTCAA GCTTGTCAAA GGTAACATAA TCAAACTGAC TTTGGATGAC
    AAGTTCATGG ACAACTGTGA TGAAAATACC CTGTGGTTGG ACTACAAGAA CATCACAAAG
    GTGGTGCAGC AGGGCAGCCA TATTTACGTG GACGATGGTC TGATTTCACT CAAGGTTAAG
    GAAATCGGAT CTGACTTCCT GAACTGTGAG ATTGAGAACG GAGGCATGTT GGGCAGCAAG
    AAAGGTGTGA ACCTGCCTGG AGCCAATGTT GACCTGCCCG CAGTGTCTGA GAAGGACATC
    AAGGACCTTC AGTTTGGTGT GGAACAGGGC GTGGACATGG TTTTTGCCTC CTTTATCCGC
    AAAGCTGCTG ATGTGCACGC TGTCAGGAAG GTGCTCGGAG AGAAAGGGAA GGATATCAGG
    ATCATCAGTA AGCTGGAGAA CCATGAGGGT GTGCGCAAGT TTGATGAGAT CCTTGAGGCC
    AGTGATGGTA TCATGGTCGC CCGTGGAGAT CTGGGTATTG AAATCCCTAC TGAAAAGGTG
    TTCCTAGCTC AGAAGATGAT GATTAGCCGT TGCAATAGGA TTGGCAAACC AATCATCTGT
    GCCACACAGA TGCTGGAGAG CATGATAAAG AAGCCTCGTC CTACCCGTGC CGAGAGCAGT
    GATGTGGCCA ATGCTGTGCT CGACGGTGCC GACTGCATCA TGCTGAGCGG AGAGACGGCA
    AAAGGAGAAT ACCCAATAGA GTCTGTGCTC ACACAGCACC TGATCGCCCG TGAAGCCGAA
    GCCGCCATGT TCCACAGGCA GTTGTTCGAA GAGCTTCGTC GTACCTCCCA TCTGACCCGT
    GATCCCACTG AGTCTGTGGC CGTTGGCGCA GTGGAAGCCT CCTTCAAGTG CTGTGCCAGC
    GCCATTATTT GCCTCACCAA GACTGGCAGG TCTGCCCAGC TGCTGTCCAG ATACAGGCCT
    CGTGCTCCTA TCATGGCTGT GACCCGTAAC GGTCAGACAT CTCGGCAGCT GCACCTGTAT
    CGTGGCGTCA TCCCCATCCT CTACACCAAG CCTGCCAATG ACATATGGGC TGAGGATGTG
    GATCTCCGTG TCAGCTTCGC ACTGGAGATC GGCAAGCATC GGAAGTACTT CAAGTCCGGT
    GATGTTATCA TCGTCGTCAC AGGCTGGCGT CCAGGATCTG GATACACCAA CACCATGCGC
    ATCGTTGTGG TGCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa57796
    Clone ID Related Accession (Same CDS sequence) XM_005163113.3 , XM_005163113.4
    Accession Version XM_005163113.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1818bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pyruvate kinase isozymes M1/M2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163113.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTCATTAT ACTCGATAAA TCGTGTTATA AATGTATTTC TACACAATAC ATGCAAAATG 
    TTCAACTGTG AAGTCAACCC AGTCCTGGCG GAAGAACGCA TTGCTGTGGG AAGTAGTTCC
    GGTCGGTATA GAGTGGATAA GTTAAGGCAG TGGCGGATGA AGAGCTCAGA AGTTCCTGCA
    GGCGTTCATC AACAGAGGAC TCTTTTAGCA GACGCAGCTC CAGCCATGTC TCAGACAAAG
    ACTACGGCCT CTGGCTTCAT TCAGACACAG CAGATGCCTG CTGCAATGGC GGAAACCTTC
    TTGGAGCATC TTTGCCTGCT GGACATCGAC TCTGAACCTA CAGTGTCTCG AAACACTGGC
    ATCGTCTGCA CACTCGGACC TGCTTCACGT TCTTTGGAGA CACTGAGAGA GATGATCTTG
    TCCGGCATGA ACGTCGCACG CCTGAACTTC TCTCACGGAA CTCACGAGTA CCATGCTGAA
    ACCATCAAGA GTGTCCGTGA AGCTATTGAG AGTTTTGGAG CTGGTACCAT TGATTACCGA
    CCTGTTGCCA TTGCCCTGGA TACCAAGGGC CCTGAGATCA GAACTGGGCT TATTAAGGGC
    AGTGGCACTG AGGAGGTCAA GCTTGTCAAA GGTAACATAA TCAAACTGAC TTTGGATGAC
    AAGTTCATGG ACAACTGTGA TGAAAATACC CTGTGGTTGG ACTACAAGAA CATCACAAAG
    GTGGTGCAGC AGGGCAGCCA TATTTACGTG GACGATGGTC TGATTTCACT CAAGGTTAAG
    GAAATCGGAT CTGACTTCCT GAACTGTGAG ATTGAGAACG GAGGCATGTT GGGCAGCAAG
    AAAGGTGTGA ACCTGCCTGG AGCCAATGTT GACCTGCCCG CAGTGTCTGA GAAGGACATC
    AAGGACCTTC AGTTTGGTGT GGAACAGGGC GTGGACATGG TTTTTGCCTC CTTTATCCGC
    AAAGCTGCTG ATGTGCACGC TGTCAGGAAG GTGCTCGGAG AGAAAGGGAA GGATATCAGG
    ATCATCAGTA AGCTGGAGAA CCATGAGGGT GTGCGCAAGT TTGATGAGAT CCTTGAGGCC
    AGTGATGGTA TCATGGTCGC CCGTGGAGAT CTGGGTATTG AAATCCCTAC TGAAAAGGTG
    TTCCTAGCTC AGAAGATGAT GATTAGCCGT TGCAATAGGA TTGGCAAACC AATCATCTGT
    GCCACACAGA TGCTGGAGAG CATGATAAAG AAGCCTCGTC CTACCCGTGC CGAGAGCAGT
    GATGTGGCCA ATGCTGTGCT CGACGGTGCC GACTGCATCA TGCTGAGCGG AGAGACGGCA
    AAAGGAGAAT ACCCAATAGA GTCTGTGCTC ACACAGCACC TGATCGCCCG TGAAGCCGAA
    GCCGCCATGT TCCACAGGCA GTTGTTCGAA GAGCTTCGTC GTACCTCCCA TCTGACCCGT
    GATCCCACTG AGTCTGTGGC CGTTGGCGCA GTGGAAGCCT CCTTCAAGTG CTGTGCCAGC
    GCCATTATTT GCCTCACCAA GACTGGCAGG TCTGCCCAGC TGCTGTCCAG ATACAGGCCT
    CGTGCTCCTA TCATGGCTGT GACCCGTAAC GGTCAGACAT CTCGGCAGCT GCACCTGTAT
    CGTGGCGTCA TCCCCATCCT CTACACCAAG CCTGCCAATG ACATATGGGC TGAGGATGTG
    GATCTCCGTG TCAGCTTCGC ACTGGAGATC GGCAAGCATC GGAAGTACTT CAAGTCCGGT
    GATGTTATCA TCGTCGTCAC AGGCTGGCGT CCAGGATCTG GATACACCAA CACCATGCGC
    ATCGTTGTGG TGCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005163170.3
    CDS308..2125
    Translation

    Target ORF information:

    RefSeq Version XM_005163113.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pyruvate kinase, muscle, b (pkmb), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005163113.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTCATTAT ACTCGATAAA TCGTGTTATA AATGTATTTC TACACAATAC ATGCAAAATG 
    TTCAACTGTG AAGTCAACCC AGTCCTGGCG GAAGAACGCA TTGCTGTGGG AAGTAGTTCC
    GGTCGGTATA GAGTGGATAA GTTAAGGCAG TGGCGGATGA AGAGCTCAGA AGTTCCTGCA
    GGCGTTCATC AACAGAGGAC TCTTTTAGCA GACGCAGCTC CAGCCATGTC TCAGACAAAG
    ACTACGGCCT CTGGCTTCAT TCAGACACAG CAGATGCCTG CTGCAATGGC GGAAACCTTC
    TTGGAGCATC TTTGCCTGCT GGACATCGAC TCTGAACCTA CAGTGTCTCG AAACACTGGC
    ATCGTCTGCA CACTCGGACC TGCTTCACGT TCTTTGGAGA CACTGAGAGA GATGATCTTG
    TCCGGCATGA ACGTCGCACG CCTGAACTTC TCTCACGGAA CTCACGAGTA CCATGCTGAA
    ACCATCAAGA GTGTCCGTGA AGCTATTGAG AGTTTTGGAG CTGGTACCAT TGATTACCGA
    CCTGTTGCCA TTGCCCTGGA TACCAAGGGC CCTGAGATCA GAACTGGGCT TATTAAGGGC
    AGTGGCACTG AGGAGGTCAA GCTTGTCAAA GGTAACATAA TCAAACTGAC TTTGGATGAC
    AAGTTCATGG ACAACTGTGA TGAAAATACC CTGTGGTTGG ACTACAAGAA CATCACAAAG
    GTGGTGCAGC AGGGCAGCCA TATTTACGTG GACGATGGTC TGATTTCACT CAAGGTTAAG
    GAAATCGGAT CTGACTTCCT GAACTGTGAG ATTGAGAACG GAGGCATGTT GGGCAGCAAG
    AAAGGTGTGA ACCTGCCTGG AGCCAATGTT GACCTGCCCG CAGTGTCTGA GAAGGACATC
    AAGGACCTTC AGTTTGGTGT GGAACAGGGC GTGGACATGG TTTTTGCCTC CTTTATCCGC
    AAAGCTGCTG ATGTGCACGC TGTCAGGAAG GTGCTCGGAG AGAAAGGGAA GGATATCAGG
    ATCATCAGTA AGCTGGAGAA CCATGAGGGT GTGCGCAAGT TTGATGAGAT CCTTGAGGCC
    AGTGATGGTA TCATGGTCGC CCGTGGAGAT CTGGGTATTG AAATCCCTAC TGAAAAGGTG
    TTCCTAGCTC AGAAGATGAT GATTAGCCGT TGCAATAGGA TTGGCAAACC AATCATCTGT
    GCCACACAGA TGCTGGAGAG CATGATAAAG AAGCCTCGTC CTACCCGTGC CGAGAGCAGT
    GATGTGGCCA ATGCTGTGCT CGACGGTGCC GACTGCATCA TGCTGAGCGG AGAGACGGCA
    AAAGGAGAAT ACCCAATAGA GTCTGTGCTC ACACAGCACC TGATCGCCCG TGAAGCCGAA
    GCCGCCATGT TCCACAGGCA GTTGTTCGAA GAGCTTCGTC GTACCTCCCA TCTGACCCGT
    GATCCCACTG AGTCTGTGGC CGTTGGCGCA GTGGAAGCCT CCTTCAAGTG CTGTGCCAGC
    GCCATTATTT GCCTCACCAA GACTGGCAGG TCTGCCCAGC TGCTGTCCAG ATACAGGCCT
    CGTGCTCCTA TCATGGCTGT GACCCGTAAC GGTCAGACAT CTCGGCAGCT GCACCTGTAT
    CGTGGCGTCA TCCCCATCCT CTACACCAAG CCTGCCAATG ACATATGGGC TGAGGATGTG
    GATCTCCGTG TCAGCTTCGC ACTGGAGATC GGCAAGCATC GGAAGTACTT CAAGTCCGGT
    GATGTTATCA TCGTCGTCAC AGGCTGGCGT CCAGGATCTG GATACACCAA CACCATGCGC
    ATCGTTGTGG TGCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.