ANO1 cDNA ORF clone, Danio rerio(Zebrafish)

The following ANO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa31017 NM_001161590.1
Latest version!
Danio rerio anoctamin 1, calcium activated chloride channel a (ano1a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa13471 XM_009303471.3
Latest version!
Danio rerio anoctamin 1, calcium activated chloride channel (ano1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa13473 XM_009303473.3
Latest version!
Danio rerio anoctamin 1, calcium activated chloride channel (ano1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa40966 XM_009303472.3
Latest version!
Danio rerio anoctamin 1, calcium activated chloride channel (ano1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa59621 XM_017356841.2
Latest version!
Danio rerio anoctamin 1, calcium activated chloride channel (ano1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
View Old Accession Versions >>
ODa13471 XM_009303471.2 Danio rerio anoctamin 1, calcium activated chloride channel (ano1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa59621 XM_017356841.1 Danio rerio anoctamin 1, calcium activated chloride channel (ano1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa40966 XM_009303472.2 Danio rerio anoctamin 1, calcium activated chloride channel (ano1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa13473 XM_009303473.2 Danio rerio anoctamin 1, calcium activated chloride channel (ano1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa31017
    Clone ID Related Accession (Same CDS sequence) NM_001161590.1
    Accession Version NM_001161590.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2778bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-21
    Organism Danio rerio(zebrafish)
    Product anoctamin-1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR318608.12 and CU928764.9. On Jun 6, 2009 this sequence version replaced XM_001923853.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTGGCGAA ATGAAAAGTA TGAATCTGTC CCGTTGGATG TGAAGGGGAT TGATGAAGAC 
    AATTCTAAAG GCACAAGGGA TTCTTTATAT CCCCACACTA CTCTCCTGAA AAGTCAGCAG
    GCGGATGAGA AACCAAAGGA CCCAAAGCTT TGGCCGTACT TCAAGGATGG TCACAGACGG
    ATAGATTATG TGCTCACCTA CCACATCCAG AAGCCCCAGA ACACTGGCAA ACAGTCATCC
    AAGTCCTGCC TGGGGGGTTG TTTGTGCTGC AAAAAACGAG GCCAGACAAA CCCACCGGTT
    CCGGACCCCG AACTAGCATC TCAGGAGCAG AGGCTGGATT ACCATGATGA CGACCAGCGG
    CTCCGCAGAG AGGAGTTTGA GGAGAACCTG CGTGAAATGG GCCTTGAGAT GGAAAAAGAG
    GAAGGGAATA AAATTCCTGG CGTGGGGTTC TTGAAGATCC ACGCTCCTTG GGAAGTTCTT
    TGTCGAGAAG CAGAGTTTAT GAAACTAAAA ATGCCCACAA TAAAGAAATA TGAAGTAAGC
    CATGGAAACA GCATTGTGGA GAAGGCCAGC AGGTTTATGC AGAAATTCAC AGCACCTTTG
    CAACCTAAGG TTGGGGACCG TCAGATTGAA CACGAGAAAC ACCTCTCATA CCCATTCTCA
    CGAGAAAAGC AGCATCTCTT TGATTTATCA GACAGGAATT CCTTCTTCGA CAGCAAAACC
    AGGAGCTCAA TAGTGTATGA GATTTTAAAG CGAACAAAAT GCACACGGGC GAAGTATATC
    ATGGGAATCA TCAGTCTTCT GGGCAACGGT ATCTACACTT CAGCCTACCC TTTGCATGAT
    GGCGATATTG ATGACGTCCA TGCTGAAATA AATGACAGAA AGATCTTATA TGAAGAGTGG
    GCGAGTTACA GCATATTCTA CAAGTATCAA CCCATCGGAC TGATCAGGAA GTATTTTGGT
    GAGAAAGTGG GCCTGTACTT CGCCTGGCTG GGTGTGTACA CTCAGATGCT CATCCCAGCA
    GCCATTGTGG GCGTGATCGT CTTCCTGTAC GGCTGCGCTA CTGTGGACGA CAACATTCCC
    AGTATGGAGA TCTGCGACGA GAGAAACAAC ATCACCATGT GCCCAATGTG CGACCGGGCC
    TGCAGCTACT GGAAACTGGT GACGGCTTGC GGCACAGCCA GAGCCAGCCA CCTGTTCGAC
    AACGCGGCCA CCGTCTTCTT TGCCATCTTC ATGGCACTAT GGGCCGCCAT GTTTATGGAG
    CACTGGAAGA GACGGCAAGT GAGGCTCAAC TACCTCTGGG ACCTGACGGG TTTTGAGGAC
    ACCGAGAGTC ATCCGAGAGC GGAGTATGAG TTTCATGTCA TGAAGAAGTC ATTGAGGAAA
    GAGAAATCTC CAAAGGAAGC AGGGAATGTG AAGCTGACAT GCACCGACAG AATGCCAGCT
    TACATGACCA TCATCATCAC CATGATTTTT CTGATCTGTG TGACCTTGGC CATCGTGTTT
    GGTGTGGTGC TATACAGGGT GTCTATTATG ACAGCCCTTC ATATGAGTTC CACTCCTACA
    TTCCGTTCTA ATATACGTGC CACCGTTAAA ACCACCGGCG CCATCATCAA CCTCATTATC
    ATCATCATCA TGGATGAGGT CTACGGAGCA GTTGCACGTT GGCTCACTAT TATGGAGGTG
    CCAAAGACTG ACAAGAGTTT TGAGGAGAGG CTGATCTTCA AGACATTCGT ATTGAAGTTT
    GCGAATGCAT TTACTCCTAT TGTGTACCTG GCGTTCTTCA GAGGCAGACT CATTGGACGT
    CCTGGCAAAT ATATCTATGT TATGGGATCT TACAGAATGG AAGAGTGCGC TCATGCCGGC
    TGTCTGATGG AACTGTGCAT TCAGCTCTGC ATCACTATGC TTGGCAAACA GCTCATCCAG
    AATAACCTGT TTGAGATCGG CGTTCCGAAA ATCAAGAAGC TGCTCCGACA ACGCAAGATT
    GACCCAAAGC ACCAGGAAGA GCTGAACAAA ACGCTTCATC GCCATGAGAA GGACCACTTC
    CTAGGACCCT TTGTCGGACT CAACCCAGAG TACATGGAAA TGATCATTCA GTTCGGGATG
    GTGACCTTAT TCGTGGCCTC TTTTCCGCTG GCTCCTCTCT TTGCTCTGCT CAACAACATC
    ATTGAGATCC GTCTTGATGC CAAAAAGTTT GTTACGGAAC TGAGAAGGCC AATCGCTGTA
    AGAGCAAAGG ACATCGGAAT CTGGTACACT CTTCTCAGAG GACTCAGCAA AGTGGCTGTC
    ATTGTCAATG CGTTTGTTAT CTCGTTCACC TCTGACTTCA TCCCGCGGCT GGTCTATCAA
    TACATGTACA GTCCAGACGG GACCCTCCAT GGCTTTGTCA ATCACACGCT CTCGTATTTT
    AACGTCACTC ACTTCCAGCC TGGCACAGAG CCCCTGGAGC CCATGCACCT GGGATACCAA
    GTGGACACGT GCAGATATAA AGATTACAGG GATCCTCCAT GGTCTGATAC ACCGTACGAG
    TTCTCAAGAG AATTCTGGGC CATTCTGGCA GCCCGCTTCG CTTTCGTAAT TCTCTTTCAG
    AATGTGGTGA TGCTGATGAG TGACTTCGTG GACTGGCTCA TCCCAGACAT CCCCAAAGAC
    ATCAGCTTGA AAATCCACAA GGAGAGGAAC CTCATCATTG AGCTGTTCAT GAAGCGGGAA
    CAGGGAAAGA AGTACAGGAA CACCATGAAC GACCCAAGTC CACAACCGCT CCGCTCGCGG
    CCCAGTTCAC AAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001155062.1
    CDS225..3002
    Translation

    Target ORF information:

    RefSeq Version NM_001161590.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio anoctamin 1, calcium activated chloride channel a (ano1a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001161590.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTGGCGAA ATGAAAAGTA TGAATCTGTC CCGTTGGATG TGAAGGGGAT TGATGAAGAC 
    AATTCTAAAG GCACAAGGGA TTCTTTATAT CCCCACACTA CTCTCCTGAA AAGTCAGCAG
    GCGGATGAGA AACCAAAGGA CCCAAAGCTT TGGCCGTACT TCAAGGATGG TCACAGACGG
    ATAGATTATG TGCTCACCTA CCACATCCAG AAGCCCCAGA ACACTGGCAA ACAGTCATCC
    AAGTCCTGCC TGGGGGGTTG TTTGTGCTGC AAAAAACGAG GCCAGACAAA CCCACCGGTT
    CCGGACCCCG AACTAGCATC TCAGGAGCAG AGGCTGGATT ACCATGATGA CGACCAGCGG
    CTCCGCAGAG AGGAGTTTGA GGAGAACCTG CGTGAAATGG GCCTTGAGAT GGAAAAAGAG
    GAAGGGAATA AAATTCCTGG CGTGGGGTTC TTGAAGATCC ACGCTCCTTG GGAAGTTCTT
    TGTCGAGAAG CAGAGTTTAT GAAACTAAAA ATGCCCACAA TAAAGAAATA TGAAGTAAGC
    CATGGAAACA GCATTGTGGA GAAGGCCAGC AGGTTTATGC AGAAATTCAC AGCACCTTTG
    CAACCTAAGG TTGGGGACCG TCAGATTGAA CACGAGAAAC ACCTCTCATA CCCATTCTCA
    CGAGAAAAGC AGCATCTCTT TGATTTATCA GACAGGAATT CCTTCTTCGA CAGCAAAACC
    AGGAGCTCAA TAGTGTATGA GATTTTAAAG CGAACAAAAT GCACACGGGC GAAGTATATC
    ATGGGAATCA TCAGTCTTCT GGGCAACGGT ATCTACACTT CAGCCTACCC TTTGCATGAT
    GGCGATATTG ATGACGTCCA TGCTGAAATA AATGACAGAA AGATCTTATA TGAAGAGTGG
    GCGAGTTACA GCATATTCTA CAAGTATCAA CCCATCGGAC TGATCAGGAA GTATTTTGGT
    GAGAAAGTGG GCCTGTACTT CGCCTGGCTG GGTGTGTACA CTCAGATGCT CATCCCAGCA
    GCCATTGTGG GCGTGATCGT CTTCCTGTAC GGCTGCGCTA CTGTGGACGA CAACATTCCC
    AGTATGGAGA TCTGCGACGA GAGAAACAAC ATCACCATGT GCCCAATGTG CGACCGGGCC
    TGCAGCTACT GGAAACTGGT GACGGCTTGC GGCACAGCCA GAGCCAGCCA CCTGTTCGAC
    AACGCGGCCA CCGTCTTCTT TGCCATCTTC ATGGCACTAT GGGCCGCCAT GTTTATGGAG
    CACTGGAAGA GACGGCAAGT GAGGCTCAAC TACCTCTGGG ACCTGACGGG TTTTGAGGAC
    ACCGAGAGTC ATCCGAGAGC GGAGTATGAG TTTCATGTCA TGAAGAAGTC ATTGAGGAAA
    GAGAAATCTC CAAAGGAAGC AGGGAATGTG AAGCTGACAT GCACCGACAG AATGCCAGCT
    TACATGACCA TCATCATCAC CATGATTTTT CTGATCTGTG TGACCTTGGC CATCGTGTTT
    GGTGTGGTGC TATACAGGGT GTCTATTATG ACAGCCCTTC ATATGAGTTC CACTCCTACA
    TTCCGTTCTA ATATACGTGC CACCGTTAAA ACCACCGGCG CCATCATCAA CCTCATTATC
    ATCATCATCA TGGATGAGGT CTACGGAGCA GTTGCACGTT GGCTCACTAT TATGGAGGTG
    CCAAAGACTG ACAAGAGTTT TGAGGAGAGG CTGATCTTCA AGACATTCGT ATTGAAGTTT
    GCGAATGCAT TTACTCCTAT TGTGTACCTG GCGTTCTTCA GAGGCAGACT CATTGGACGT
    CCTGGCAAAT ATATCTATGT TATGGGATCT TACAGAATGG AAGAGTGCGC TCATGCCGGC
    TGTCTGATGG AACTGTGCAT TCAGCTCTGC ATCACTATGC TTGGCAAACA GCTCATCCAG
    AATAACCTGT TTGAGATCGG CGTTCCGAAA ATCAAGAAGC TGCTCCGACA ACGCAAGATT
    GACCCAAAGC ACCAGGAAGA GCTGAACAAA ACGCTTCATC GCCATGAGAA GGACCACTTC
    CTAGGACCCT TTGTCGGACT CAACCCAGAG TACATGGAAA TGATCATTCA GTTCGGGATG
    GTGACCTTAT TCGTGGCCTC TTTTCCGCTG GCTCCTCTCT TTGCTCTGCT CAACAACATC
    ATTGAGATCC GTCTTGATGC CAAAAAGTTT GTTACGGAAC TGAGAAGGCC AATCGCTGTA
    AGAGCAAAGG ACATCGGAAT CTGGTACACT CTTCTCAGAG GACTCAGCAA AGTGGCTGTC
    ATTGTCAATG CGTTTGTTAT CTCGTTCACC TCTGACTTCA TCCCGCGGCT GGTCTATCAA
    TACATGTACA GTCCAGACGG GACCCTCCAT GGCTTTGTCA ATCACACGCT CTCGTATTTT
    AACGTCACTC ACTTCCAGCC TGGCACAGAG CCCCTGGAGC CCATGCACCT GGGATACCAA
    GTGGACACGT GCAGATATAA AGATTACAGG GATCCTCCAT GGTCTGATAC ACCGTACGAG
    TTCTCAAGAG AATTCTGGGC CATTCTGGCA GCCCGCTTCG CTTTCGTAAT TCTCTTTCAG
    AATGTGGTGA TGCTGATGAG TGACTTCGTG GACTGGCTCA TCCCAGACAT CCCCAAAGAC
    ATCAGCTTGA AAATCCACAA GGAGAGGAAC CTCATCATTG AGCTGTTCAT GAAGCGGGAA
    CAGGGAAAGA AGTACAGGAA CACCATGAAC GACCCAAGTC CACAACCGCT CCGCTCGCGG
    CCCAGTTCAC AAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13471
    Clone ID Related Accession (Same CDS sequence) XM_009303471.3 , XM_009303471.2
    Accession Version XM_009303471.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2901bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product anoctamin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303471.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAGGGTG AAGATGATGT AAATACTGCG AGGAAAGATG ATGAGGCTGA CGGCTCGGGT 
    GAGAAACTCT TGGGTTTCAA CAGCACAGAG GAGCAGCCGT CAGGACAGGA GAGCATGTGG
    CGAAATGAAA AGTATGAATC TGTCCCGTTG GATGTGAAGG GGATTGATGA AGACAATTCT
    AAAGGCACAA GGGATTCTTT ATATCCCCAC ACTACTCTCC TGAAAAGTCA GCAGGCGGAT
    GAGAAACCAA AGGACCCAAA GCTTTGGCCG TACTTCAAGG ATGGTCACAG ACGGATAGAT
    TATGTGCTCA CCTACCACAT CCAGAAGCCC CAGAACACTG GCAAACAGTC ATCCAAGTCC
    TGCCTGGGGG GTTGTTTGTG CTGCAAAAAA CGAGGCCAGA CAAACCCACC GGTTCCGGAC
    CCCGAACTAG CATCTCAGGA GCAGAGGCTG GATTACCATG ATGACGACCA GCGGCTCCGC
    AGAGAGGAGT TTGAGGAGAA CCTGCGTGAA ATGGGCCTTG AGATGGAAAA AGAGGAAGGG
    AATAAAATTC CTGGCGTGGG GTTCTTGAAG ATCCACGCTC CTTGGGAAGT TCTTTGTCGA
    GAAGCAGAGT TTATGAAACT AAAAATGCCC ACAATAAAGA AATATGAAGT AAGCCATGGA
    AACAGCATTG TGGAGAAGGC CAGCAGGTTT ATGCAGAAAT TCACAGCACC TTTGCAACCT
    AAGGTTGGGG ACCGTCAGAT TGAACACGAG AAACACCTCT CATACCCATT CTCACGAGAA
    AAGCAGCATC TCTTTGATTT ATCAGACAGG AATTCCTTCT TCGACAGCAA AACCAGGAGC
    TCAATAGTGT ATGAGATTTT AAAGCGAACA AAATGCACAC GGGCGAAGTA TATCATGGGA
    ATCATCAGTC TTCTGGGCAA CGGTATCTAC ACTTCAGCCT ACCCTTTGCA TGATGGCGAT
    ATTGATGACG TCCATGCTGA AATAAATGAC AGAAAGATCT TATATGAAGA GTGGGCGAGT
    TACAGCATAT TCTACAAGTA TCAACCCATC GGACTGATCA GGAAGTATTT TGGTGAGAAA
    GTGGGCCTGT ACTTCGCCTG GCTGGGTGTG TACACTCAGA TGCTCATCCC AGCAGCCATT
    GTGGGCGTGA TCGTCTTCCT GTACGGCTGC GCTACTGTGG ACGACAACAT TCCCAGTATG
    GAGATCTGCG ACGAGAGAAA CAACATCACC ATGTGCCCAA TGTGCGACCG GGCCTGCAGC
    TACTGGAAAC TGGTGACGGC TTGCGGCACA GCCAGAGCCA GCCACCTGTT CGACAACGCG
    GCCACCGTCT TCTTTGCCAT CTTCATGGCA CTATGGGCCG CCATGTTTAT GGAGCACTGG
    AAGAGACGGC AAGTGAGGCT CAACTACCTC TGGGACCTGA CGGGTTTTGA GGACACCGAG
    GGGAAACAAA AGAGTCATCC GAGAGCGGAG TATGAGTTTC ATGTCATGAA GAAGTCATTG
    AGGAAAGAGA AATCTCCAAA GGCAGGGAAT GTGAAGCTGA CATGCACCGA CAGAATGCCA
    GCTTACATGA CCATCATCAT CACCATGATT TTTCTGATCT GTGTGACCTT GGCCATCGTG
    TTTGGTGTGG TGCTATACAG GGTGTCTATT ATGACAGCCC TTCATATGAG TTCCACTCCT
    ACATTCCGTT CTAATATACG TGCCACCGTT AAAACCACCG GCGCCATCAT CAACCTCATT
    ATCATCATCA TCATGGATGA GGTCTACGGA GCAGTTGCAC GTTGGCTCAC TATTATGGAG
    GTGCCAAAGA CTGACAAGAG TTTTGAGGAG AGGCTGATCT TCAAGACATT CGTATTGAAG
    TTTGCGAATG CATTTACTCC TATTGTGTAC CTGGCGTTCT TCAGAGGCAG ACTCATTGGA
    CGTCCTGGCA AATATATCTA TGTTATGGGA TCTTACAGAA TGGAAGAGTG CGCTCATGCC
    GGCTGTCTGA TGGAACTGTG CATTCAGCTC TGCATCACTA TGCTTGGCAA ACAGCTCATC
    CAGAATAACC TGTTTGAGAT CGGCGTTCCG AAAATCAAGA AGCTGCTCCG ACAACGCAAG
    ATTGACCCAA AGCACCAGGA AGAGCTGAAC AAAACGCTTC ATCGCCATGA GAAGGACCAC
    TTCCTAGGAC CCTTTGTCGG ACTCAACCCA GAGTACATGG AAATGATCAT TCAGTTCGGG
    ATGGTGACCT TATTCGTGGC CTCTTTTCCG CTGGCTCCTC TCTTTGCTCT GCTCAACAAC
    ATCATTGAGA TCCGTCTTGA TGCCAAAAAG TTTGTTACGG AACTGAGAAG GCCAATCGCT
    GTAAGAGCAA AGGACATCGG AATCTGGTAC ACTCTTCTCA GAGGACTCAG CAAAGTGGCT
    GTCATTGTCA ATGCGTTTGT TATCTCGTTC ACCTCTGACT TCATCCCGCG GCTGGTCTAT
    CAATACATGT ACAGTCCAGA CGGGACCCTC CATGGCTTTG TCAATCACAC GCTCTCGTAT
    TTTAACGTCA CTCACTTCCA GCCTGGCACA GAGCCCCTGG AGCCCATGCA CCTGGGATAC
    CAAGTGGACA CGTGCAGATA TAAAGATTAC AGGGATCCTC CATGGTCTGA TACACCGTAC
    GAGTTCTCAA GAGAATTCTG GGCCATTCTG GCAGCCCGCT TCGCTTTCGT AATTCTCTTT
    CAGAATGTGG TGATGCTGAT GAGTGACTTC GTGGACTGGC TCATCCCAGA CATCCCCAAA
    GACATCAGCT TGAAAATCCA CAAGGAGAGG AACCTCATCA TTGAGCTGTT CATGAAGCGG
    GAACAGGGAA AGAAGTACAG GAACACCATG AACGACCCAA GTCCACAACC GCTCCGCTCG
    CGGCCCAGTT CACAAGCGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301746.1
    CDS208..3108
    Translation

    Target ORF information:

    RefSeq Version XM_009303471.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio anoctamin 1, calcium activated chloride channel (ano1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303471.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAGGGTG AAGATGATGT AAATACTGCG AGGAAAGATG ATGAGGCTGA CGGCTCGGGT 
    GAGAAACTCT TGGGTTTCAA CAGCACAGAG GAGCAGCCGT CAGGACAGGA GAGCATGTGG
    CGAAATGAAA AGTATGAATC TGTCCCGTTG GATGTGAAGG GGATTGATGA AGACAATTCT
    AAAGGCACAA GGGATTCTTT ATATCCCCAC ACTACTCTCC TGAAAAGTCA GCAGGCGGAT
    GAGAAACCAA AGGACCCAAA GCTTTGGCCG TACTTCAAGG ATGGTCACAG ACGGATAGAT
    TATGTGCTCA CCTACCACAT CCAGAAGCCC CAGAACACTG GCAAACAGTC ATCCAAGTCC
    TGCCTGGGGG GTTGTTTGTG CTGCAAAAAA CGAGGCCAGA CAAACCCACC GGTTCCGGAC
    CCCGAACTAG CATCTCAGGA GCAGAGGCTG GATTACCATG ATGACGACCA GCGGCTCCGC
    AGAGAGGAGT TTGAGGAGAA CCTGCGTGAA ATGGGCCTTG AGATGGAAAA AGAGGAAGGG
    AATAAAATTC CTGGCGTGGG GTTCTTGAAG ATCCACGCTC CTTGGGAAGT TCTTTGTCGA
    GAAGCAGAGT TTATGAAACT AAAAATGCCC ACAATAAAGA AATATGAAGT AAGCCATGGA
    AACAGCATTG TGGAGAAGGC CAGCAGGTTT ATGCAGAAAT TCACAGCACC TTTGCAACCT
    AAGGTTGGGG ACCGTCAGAT TGAACACGAG AAACACCTCT CATACCCATT CTCACGAGAA
    AAGCAGCATC TCTTTGATTT ATCAGACAGG AATTCCTTCT TCGACAGCAA AACCAGGAGC
    TCAATAGTGT ATGAGATTTT AAAGCGAACA AAATGCACAC GGGCGAAGTA TATCATGGGA
    ATCATCAGTC TTCTGGGCAA CGGTATCTAC ACTTCAGCCT ACCCTTTGCA TGATGGCGAT
    ATTGATGACG TCCATGCTGA AATAAATGAC AGAAAGATCT TATATGAAGA GTGGGCGAGT
    TACAGCATAT TCTACAAGTA TCAACCCATC GGACTGATCA GGAAGTATTT TGGTGAGAAA
    GTGGGCCTGT ACTTCGCCTG GCTGGGTGTG TACACTCAGA TGCTCATCCC AGCAGCCATT
    GTGGGCGTGA TCGTCTTCCT GTACGGCTGC GCTACTGTGG ACGACAACAT TCCCAGTATG
    GAGATCTGCG ACGAGAGAAA CAACATCACC ATGTGCCCAA TGTGCGACCG GGCCTGCAGC
    TACTGGAAAC TGGTGACGGC TTGCGGCACA GCCAGAGCCA GCCACCTGTT CGACAACGCG
    GCCACCGTCT TCTTTGCCAT CTTCATGGCA CTATGGGCCG CCATGTTTAT GGAGCACTGG
    AAGAGACGGC AAGTGAGGCT CAACTACCTC TGGGACCTGA CGGGTTTTGA GGACACCGAG
    GGGAAACAAA AGAGTCATCC GAGAGCGGAG TATGAGTTTC ATGTCATGAA GAAGTCATTG
    AGGAAAGAGA AATCTCCAAA GGCAGGGAAT GTGAAGCTGA CATGCACCGA CAGAATGCCA
    GCTTACATGA CCATCATCAT CACCATGATT TTTCTGATCT GTGTGACCTT GGCCATCGTG
    TTTGGTGTGG TGCTATACAG GGTGTCTATT ATGACAGCCC TTCATATGAG TTCCACTCCT
    ACATTCCGTT CTAATATACG TGCCACCGTT AAAACCACCG GCGCCATCAT CAACCTCATT
    ATCATCATCA TCATGGATGA GGTCTACGGA GCAGTTGCAC GTTGGCTCAC TATTATGGAG
    GTGCCAAAGA CTGACAAGAG TTTTGAGGAG AGGCTGATCT TCAAGACATT CGTATTGAAG
    TTTGCGAATG CATTTACTCC TATTGTGTAC CTGGCGTTCT TCAGAGGCAG ACTCATTGGA
    CGTCCTGGCA AATATATCTA TGTTATGGGA TCTTACAGAA TGGAAGAGTG CGCTCATGCC
    GGCTGTCTGA TGGAACTGTG CATTCAGCTC TGCATCACTA TGCTTGGCAA ACAGCTCATC
    CAGAATAACC TGTTTGAGAT CGGCGTTCCG AAAATCAAGA AGCTGCTCCG ACAACGCAAG
    ATTGACCCAA AGCACCAGGA AGAGCTGAAC AAAACGCTTC ATCGCCATGA GAAGGACCAC
    TTCCTAGGAC CCTTTGTCGG ACTCAACCCA GAGTACATGG AAATGATCAT TCAGTTCGGG
    ATGGTGACCT TATTCGTGGC CTCTTTTCCG CTGGCTCCTC TCTTTGCTCT GCTCAACAAC
    ATCATTGAGA TCCGTCTTGA TGCCAAAAAG TTTGTTACGG AACTGAGAAG GCCAATCGCT
    GTAAGAGCAA AGGACATCGG AATCTGGTAC ACTCTTCTCA GAGGACTCAG CAAAGTGGCT
    GTCATTGTCA ATGCGTTTGT TATCTCGTTC ACCTCTGACT TCATCCCGCG GCTGGTCTAT
    CAATACATGT ACAGTCCAGA CGGGACCCTC CATGGCTTTG TCAATCACAC GCTCTCGTAT
    TTTAACGTCA CTCACTTCCA GCCTGGCACA GAGCCCCTGG AGCCCATGCA CCTGGGATAC
    CAAGTGGACA CGTGCAGATA TAAAGATTAC AGGGATCCTC CATGGTCTGA TACACCGTAC
    GAGTTCTCAA GAGAATTCTG GGCCATTCTG GCAGCCCGCT TCGCTTTCGT AATTCTCTTT
    CAGAATGTGG TGATGCTGAT GAGTGACTTC GTGGACTGGC TCATCCCAGA CATCCCCAAA
    GACATCAGCT TGAAAATCCA CAAGGAGAGG AACCTCATCA TTGAGCTGTT CATGAAGCGG
    GAACAGGGAA AGAAGTACAG GAACACCATG AACGACCCAA GTCCACAACC GCTCCGCTCG
    CGGCCCAGTT CACAAGCGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13471
    Clone ID Related Accession (Same CDS sequence) XM_009303471.3 , XM_009303471.2
    Accession Version XM_009303471.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2901bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product anoctamin-1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303471.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAGGGTG AAGATGATGT AAATACTGCG AGGAAAGATG ATGAGGCTGA CGGCTCGGGT 
    GAGAAACTCT TGGGTTTCAA CAGCACAGAG GAGCAGCCGT CAGGACAGGA GAGCATGTGG
    CGAAATGAAA AGTATGAATC TGTCCCGTTG GATGTGAAGG GGATTGATGA AGACAATTCT
    AAAGGCACAA GGGATTCTTT ATATCCCCAC ACTACTCTCC TGAAAAGTCA GCAGGCGGAT
    GAGAAACCAA AGGACCCAAA GCTTTGGCCG TACTTCAAGG ATGGTCACAG ACGGATAGAT
    TATGTGCTCA CCTACCACAT CCAGAAGCCC CAGAACACTG GCAAACAGTC ATCCAAGTCC
    TGCCTGGGGG GTTGTTTGTG CTGCAAAAAA CGAGGCCAGA CAAACCCACC GGTTCCGGAC
    CCCGAACTAG CATCTCAGGA GCAGAGGCTG GATTACCATG ATGACGACCA GCGGCTCCGC
    AGAGAGGAGT TTGAGGAGAA CCTGCGTGAA ATGGGCCTTG AGATGGAAAA AGAGGAAGGG
    AATAAAATTC CTGGCGTGGG GTTCTTGAAG ATCCACGCTC CTTGGGAAGT TCTTTGTCGA
    GAAGCAGAGT TTATGAAACT AAAAATGCCC ACAATAAAGA AATATGAAGT AAGCCATGGA
    AACAGCATTG TGGAGAAGGC CAGCAGGTTT ATGCAGAAAT TCACAGCACC TTTGCAACCT
    AAGGTTGGGG ACCGTCAGAT TGAACACGAG AAACACCTCT CATACCCATT CTCACGAGAA
    AAGCAGCATC TCTTTGATTT ATCAGACAGG AATTCCTTCT TCGACAGCAA AACCAGGAGC
    TCAATAGTGT ATGAGATTTT AAAGCGAACA AAATGCACAC GGGCGAAGTA TATCATGGGA
    ATCATCAGTC TTCTGGGCAA CGGTATCTAC ACTTCAGCCT ACCCTTTGCA TGATGGCGAT
    ATTGATGACG TCCATGCTGA AATAAATGAC AGAAAGATCT TATATGAAGA GTGGGCGAGT
    TACAGCATAT TCTACAAGTA TCAACCCATC GGACTGATCA GGAAGTATTT TGGTGAGAAA
    GTGGGCCTGT ACTTCGCCTG GCTGGGTGTG TACACTCAGA TGCTCATCCC AGCAGCCATT
    GTGGGCGTGA TCGTCTTCCT GTACGGCTGC GCTACTGTGG ACGACAACAT TCCCAGTATG
    GAGATCTGCG ACGAGAGAAA CAACATCACC ATGTGCCCAA TGTGCGACCG GGCCTGCAGC
    TACTGGAAAC TGGTGACGGC TTGCGGCACA GCCAGAGCCA GCCACCTGTT CGACAACGCG
    GCCACCGTCT TCTTTGCCAT CTTCATGGCA CTATGGGCCG CCATGTTTAT GGAGCACTGG
    AAGAGACGGC AAGTGAGGCT CAACTACCTC TGGGACCTGA CGGGTTTTGA GGACACCGAG
    GGGAAACAAA AGAGTCATCC GAGAGCGGAG TATGAGTTTC ATGTCATGAA GAAGTCATTG
    AGGAAAGAGA AATCTCCAAA GGCAGGGAAT GTGAAGCTGA CATGCACCGA CAGAATGCCA
    GCTTACATGA CCATCATCAT CACCATGATT TTTCTGATCT GTGTGACCTT GGCCATCGTG
    TTTGGTGTGG TGCTATACAG GGTGTCTATT ATGACAGCCC TTCATATGAG TTCCACTCCT
    ACATTCCGTT CTAATATACG TGCCACCGTT AAAACCACCG GCGCCATCAT CAACCTCATT
    ATCATCATCA TCATGGATGA GGTCTACGGA GCAGTTGCAC GTTGGCTCAC TATTATGGAG
    GTGCCAAAGA CTGACAAGAG TTTTGAGGAG AGGCTGATCT TCAAGACATT CGTATTGAAG
    TTTGCGAATG CATTTACTCC TATTGTGTAC CTGGCGTTCT TCAGAGGCAG ACTCATTGGA
    CGTCCTGGCA AATATATCTA TGTTATGGGA TCTTACAGAA TGGAAGAGTG CGCTCATGCC
    GGCTGTCTGA TGGAACTGTG CATTCAGCTC TGCATCACTA TGCTTGGCAA ACAGCTCATC
    CAGAATAACC TGTTTGAGAT CGGCGTTCCG AAAATCAAGA AGCTGCTCCG ACAACGCAAG
    ATTGACCCAA AGCACCAGGA AGAGCTGAAC AAAACGCTTC ATCGCCATGA GAAGGACCAC
    TTCCTAGGAC CCTTTGTCGG ACTCAACCCA GAGTACATGG AAATGATCAT TCAGTTCGGG
    ATGGTGACCT TATTCGTGGC CTCTTTTCCG CTGGCTCCTC TCTTTGCTCT GCTCAACAAC
    ATCATTGAGA TCCGTCTTGA TGCCAAAAAG TTTGTTACGG AACTGAGAAG GCCAATCGCT
    GTAAGAGCAA AGGACATCGG AATCTGGTAC ACTCTTCTCA GAGGACTCAG CAAAGTGGCT
    GTCATTGTCA ATGCGTTTGT TATCTCGTTC ACCTCTGACT TCATCCCGCG GCTGGTCTAT
    CAATACATGT ACAGTCCAGA CGGGACCCTC CATGGCTTTG TCAATCACAC GCTCTCGTAT
    TTTAACGTCA CTCACTTCCA GCCTGGCACA GAGCCCCTGG AGCCCATGCA CCTGGGATAC
    CAAGTGGACA CGTGCAGATA TAAAGATTAC AGGGATCCTC CATGGTCTGA TACACCGTAC
    GAGTTCTCAA GAGAATTCTG GGCCATTCTG GCAGCCCGCT TCGCTTTCGT AATTCTCTTT
    CAGAATGTGG TGATGCTGAT GAGTGACTTC GTGGACTGGC TCATCCCAGA CATCCCCAAA
    GACATCAGCT TGAAAATCCA CAAGGAGAGG AACCTCATCA TTGAGCTGTT CATGAAGCGG
    GAACAGGGAA AGAAGTACAG GAACACCATG AACGACCCAA GTCCACAACC GCTCCGCTCG
    CGGCCCAGTT CACAAGCGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301746.1
    CDS199..3099
    Translation

    Target ORF information:

    RefSeq Version XM_009303471.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio anoctamin 1, calcium activated chloride channel (ano1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303471.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAGGGTG AAGATGATGT AAATACTGCG AGGAAAGATG ATGAGGCTGA CGGCTCGGGT 
    GAGAAACTCT TGGGTTTCAA CAGCACAGAG GAGCAGCCGT CAGGACAGGA GAGCATGTGG
    CGAAATGAAA AGTATGAATC TGTCCCGTTG GATGTGAAGG GGATTGATGA AGACAATTCT
    AAAGGCACAA GGGATTCTTT ATATCCCCAC ACTACTCTCC TGAAAAGTCA GCAGGCGGAT
    GAGAAACCAA AGGACCCAAA GCTTTGGCCG TACTTCAAGG ATGGTCACAG ACGGATAGAT
    TATGTGCTCA CCTACCACAT CCAGAAGCCC CAGAACACTG GCAAACAGTC ATCCAAGTCC
    TGCCTGGGGG GTTGTTTGTG CTGCAAAAAA CGAGGCCAGA CAAACCCACC GGTTCCGGAC
    CCCGAACTAG CATCTCAGGA GCAGAGGCTG GATTACCATG ATGACGACCA GCGGCTCCGC
    AGAGAGGAGT TTGAGGAGAA CCTGCGTGAA ATGGGCCTTG AGATGGAAAA AGAGGAAGGG
    AATAAAATTC CTGGCGTGGG GTTCTTGAAG ATCCACGCTC CTTGGGAAGT TCTTTGTCGA
    GAAGCAGAGT TTATGAAACT AAAAATGCCC ACAATAAAGA AATATGAAGT AAGCCATGGA
    AACAGCATTG TGGAGAAGGC CAGCAGGTTT ATGCAGAAAT TCACAGCACC TTTGCAACCT
    AAGGTTGGGG ACCGTCAGAT TGAACACGAG AAACACCTCT CATACCCATT CTCACGAGAA
    AAGCAGCATC TCTTTGATTT ATCAGACAGG AATTCCTTCT TCGACAGCAA AACCAGGAGC
    TCAATAGTGT ATGAGATTTT AAAGCGAACA AAATGCACAC GGGCGAAGTA TATCATGGGA
    ATCATCAGTC TTCTGGGCAA CGGTATCTAC ACTTCAGCCT ACCCTTTGCA TGATGGCGAT
    ATTGATGACG TCCATGCTGA AATAAATGAC AGAAAGATCT TATATGAAGA GTGGGCGAGT
    TACAGCATAT TCTACAAGTA TCAACCCATC GGACTGATCA GGAAGTATTT TGGTGAGAAA
    GTGGGCCTGT ACTTCGCCTG GCTGGGTGTG TACACTCAGA TGCTCATCCC AGCAGCCATT
    GTGGGCGTGA TCGTCTTCCT GTACGGCTGC GCTACTGTGG ACGACAACAT TCCCAGTATG
    GAGATCTGCG ACGAGAGAAA CAACATCACC ATGTGCCCAA TGTGCGACCG GGCCTGCAGC
    TACTGGAAAC TGGTGACGGC TTGCGGCACA GCCAGAGCCA GCCACCTGTT CGACAACGCG
    GCCACCGTCT TCTTTGCCAT CTTCATGGCA CTATGGGCCG CCATGTTTAT GGAGCACTGG
    AAGAGACGGC AAGTGAGGCT CAACTACCTC TGGGACCTGA CGGGTTTTGA GGACACCGAG
    GGGAAACAAA AGAGTCATCC GAGAGCGGAG TATGAGTTTC ATGTCATGAA GAAGTCATTG
    AGGAAAGAGA AATCTCCAAA GGCAGGGAAT GTGAAGCTGA CATGCACCGA CAGAATGCCA
    GCTTACATGA CCATCATCAT CACCATGATT TTTCTGATCT GTGTGACCTT GGCCATCGTG
    TTTGGTGTGG TGCTATACAG GGTGTCTATT ATGACAGCCC TTCATATGAG TTCCACTCCT
    ACATTCCGTT CTAATATACG TGCCACCGTT AAAACCACCG GCGCCATCAT CAACCTCATT
    ATCATCATCA TCATGGATGA GGTCTACGGA GCAGTTGCAC GTTGGCTCAC TATTATGGAG
    GTGCCAAAGA CTGACAAGAG TTTTGAGGAG AGGCTGATCT TCAAGACATT CGTATTGAAG
    TTTGCGAATG CATTTACTCC TATTGTGTAC CTGGCGTTCT TCAGAGGCAG ACTCATTGGA
    CGTCCTGGCA AATATATCTA TGTTATGGGA TCTTACAGAA TGGAAGAGTG CGCTCATGCC
    GGCTGTCTGA TGGAACTGTG CATTCAGCTC TGCATCACTA TGCTTGGCAA ACAGCTCATC
    CAGAATAACC TGTTTGAGAT CGGCGTTCCG AAAATCAAGA AGCTGCTCCG ACAACGCAAG
    ATTGACCCAA AGCACCAGGA AGAGCTGAAC AAAACGCTTC ATCGCCATGA GAAGGACCAC
    TTCCTAGGAC CCTTTGTCGG ACTCAACCCA GAGTACATGG AAATGATCAT TCAGTTCGGG
    ATGGTGACCT TATTCGTGGC CTCTTTTCCG CTGGCTCCTC TCTTTGCTCT GCTCAACAAC
    ATCATTGAGA TCCGTCTTGA TGCCAAAAAG TTTGTTACGG AACTGAGAAG GCCAATCGCT
    GTAAGAGCAA AGGACATCGG AATCTGGTAC ACTCTTCTCA GAGGACTCAG CAAAGTGGCT
    GTCATTGTCA ATGCGTTTGT TATCTCGTTC ACCTCTGACT TCATCCCGCG GCTGGTCTAT
    CAATACATGT ACAGTCCAGA CGGGACCCTC CATGGCTTTG TCAATCACAC GCTCTCGTAT
    TTTAACGTCA CTCACTTCCA GCCTGGCACA GAGCCCCTGG AGCCCATGCA CCTGGGATAC
    CAAGTGGACA CGTGCAGATA TAAAGATTAC AGGGATCCTC CATGGTCTGA TACACCGTAC
    GAGTTCTCAA GAGAATTCTG GGCCATTCTG GCAGCCCGCT TCGCTTTCGT AATTCTCTTT
    CAGAATGTGG TGATGCTGAT GAGTGACTTC GTGGACTGGC TCATCCCAGA CATCCCCAAA
    GACATCAGCT TGAAAATCCA CAAGGAGAGG AACCTCATCA TTGAGCTGTT CATGAAGCGG
    GAACAGGGAA AGAAGTACAG GAACACCATG AACGACCCAA GTCCACAACC GCTCCGCTCG
    CGGCCCAGTT CACAAGCGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13473
    Clone ID Related Accession (Same CDS sequence) XM_009303473.3 , XM_009303473.2
    Accession Version XM_009303473.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2787bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product anoctamin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303473.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTGGCGAA ATGAAAAGTA TGAATCTGTC CCGTTGGATG TGAAGGGGAT TGATGAAGAC 
    AATTCTAAAG GCACAAGGGA TTCTTTATAT CCCCACACTA CTCTCCTGAA AAGTCAGCAG
    GCGGATGAGA AACCAAAGGA CCCAAAGCTT TGGCCGTACT TCAAGGATGG TCACAGACGG
    ATAGATTATG TGCTCACCTA CCACATCCAG AAGCCCCAGA ACACTGGCAA ACAGTCATCC
    AAGTCCTGCC TGGGGGGTTG TTTGTGCTGC AAAAAACGAG GCCAGACAAA CCCACCGGTT
    CCGGACCCCG AACTAGCATC TCAGGAGCAG AGGCTGGATT ACCATGATGA CGACCAGCGG
    CTCCGCAGAG AGGAGTTTGA GGAGAACCTG CGTGAAATGG GCCTTGAGAT GGAAAAAGAG
    GAAGGGAATA AAATTCCTGG CGTGGGGTTC TTGAAGATCC ACGCTCCTTG GGAAGTTCTT
    TGTCGAGAAG CAGAGTTTAT GAAACTAAAA ATGCCCACAA TAAAGAAATA TGAAGTAAGC
    CATGGAAACA GCATTGTGGA GAAGGCCAGC AGGTTTATGC AGAAATTCAC AGCACCTTTG
    CAACCTAAGG TTGGGGACCG TCAGATTGAA CACGAGAAAC ACCTCTCATA CCCATTCTCA
    CGAGAAAAGC AGCATCTCTT TGATTTATCA GACAGGAATT CCTTCTTCGA CAGCAAAACC
    AGGAGCTCAA TAGTGTATGA GATTTTAAAG CGAACAAAAT GCACACGGGC GAAGTATATC
    ATGGGAATCA TCAGTCTTCT GGGCAACGGT ATCTACACTT CAGCCTACCC TTTGCATGAT
    GGCGATATTG ATGACGTCCA TGCTGAAATA AATGACAGAA AGATCTTATA TGAAGAGTGG
    GCGAGTTACA GCATATTCTA CAAGTATCAA CCCATCGGAC TGATCAGGAA GTATTTTGGT
    GAGAAAGTGG GCCTGTACTT CGCCTGGCTG GGTGTGTACA CTCAGATGCT CATCCCAGCA
    GCCATTGTGG GCGTGATCGT CTTCCTGTAC GGCTGCGCTA CTGTGGACGA CAACATTCCC
    AGTATGGAGA TCTGCGACGA GAGAAACAAC ATCACCATGT GCCCAATGTG CGACCGGGCC
    TGCAGCTACT GGAAACTGGT GACGGCTTGC GGCACAGCCA GAGCCAGCCA CCTGTTCGAC
    AACGCGGCCA CCGTCTTCTT TGCCATCTTC ATGGCACTAT GGGCCGCCAT GTTTATGGAG
    CACTGGAAGA GACGGCAAGT GAGGCTCAAC TACCTCTGGG ACCTGACGGG TTTTGAGGAC
    ACCGAGGGGA AACAAAAGAG TCATCCGAGA GCGGAGTATG AGTTTCATGT CATGAAGAAG
    TCATTGAGGA AAGAGAAATC TCCAAAGGCA GGGAATGTGA AGCTGACATG CACCGACAGA
    ATGCCAGCTT ACATGACCAT CATCATCACC ATGATTTTTC TGATCTGTGT GACCTTGGCC
    ATCGTGTTTG GTGTGGTGCT ATACAGGGTG TCTATTATGA CAGCCCTTCA TATGAGTTCC
    ACTCCTACAT TCCGTTCTAA TATACGTGCC ACCGTTAAAA CCACCGGCGC CATCATCAAC
    CTCATTATCA TCATCATCAT GGATGAGGTC TACGGAGCAG TTGCACGTTG GCTCACTATT
    ATGGAGGTGC CAAAGACTGA CAAGAGTTTT GAGGAGAGGC TGATCTTCAA GACATTCGTA
    TTGAAGTTTG CGAATGCATT TACTCCTATT GTGTACCTGG CGTTCTTCAG AGGCAGACTC
    ATTGGACGTC CTGGCAAATA TATCTATGTT ATGGGATCTT ACAGAATGGA AGAGTGCGCT
    CATGCCGGCT GTCTGATGGA ACTGTGCATT CAGCTCTGCA TCACTATGCT TGGCAAACAG
    CTCATCCAGA ATAACCTGTT TGAGATCGGC GTTCCGAAAA TCAAGAAGCT GCTCCGACAA
    CGCAAGATTG ACCCAAAGCA CCAGGAAGAG CTGAACAAAA CGCTTCATCG CCATGAGAAG
    GACCACTTCC TAGGACCCTT TGTCGGACTC AACCCAGAGT ACATGGAAAT GATCATTCAG
    TTCGGGATGG TGACCTTATT CGTGGCCTCT TTTCCGCTGG CTCCTCTCTT TGCTCTGCTC
    AACAACATCA TTGAGATCCG TCTTGATGCC AAAAAGTTTG TTACGGAACT GAGAAGGCCA
    ATCGCTGTAA GAGCAAAGGA CATCGGAATC TGGTACACTC TTCTCAGAGG ACTCAGCAAA
    GTGGCTGTCA TTGTCAATGC GTTTGTTATC TCGTTCACCT CTGACTTCAT CCCGCGGCTG
    GTCTATCAAT ACATGTACAG TCCAGACGGG ACCCTCCATG GCTTTGTCAA TCACACGCTC
    TCGTATTTTA ACGTCACTCA CTTCCAGCCT GGCACAGAGC CCCTGGAGCC CATGCACCTG
    GGATACCAAG TGGACACGTG CAGATATAAA GATTACAGGG ATCCTCCATG GTCTGATACA
    CCGTACGAGT TCTCAAGAGA ATTCTGGGCC ATTCTGGCAG CCCGCTTCGC TTTCGTAATT
    CTCTTTCAGA ATGTGGTGAT GCTGATGAGT GACTTCGTGG ACTGGCTCAT CCCAGACATC
    CCCAAAGACA TCAGCTTGAA AATCCACAAG GAGAGGAACC TCATCATTGA GCTGTTCATG
    AAGCGGGAAC AGGGAAAGAA GTACAGGAAC ACCATGAACG ACCCAAGTCC ACAACCGCTC
    CGCTCGCGGC CCAGTTCACA AGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301748.1
    CDS298..3084
    Translation

    Target ORF information:

    RefSeq Version XM_009303473.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio anoctamin 1, calcium activated chloride channel (ano1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303473.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTGGCGAA ATGAAAAGTA TGAATCTGTC CCGTTGGATG TGAAGGGGAT TGATGAAGAC 
    AATTCTAAAG GCACAAGGGA TTCTTTATAT CCCCACACTA CTCTCCTGAA AAGTCAGCAG
    GCGGATGAGA AACCAAAGGA CCCAAAGCTT TGGCCGTACT TCAAGGATGG TCACAGACGG
    ATAGATTATG TGCTCACCTA CCACATCCAG AAGCCCCAGA ACACTGGCAA ACAGTCATCC
    AAGTCCTGCC TGGGGGGTTG TTTGTGCTGC AAAAAACGAG GCCAGACAAA CCCACCGGTT
    CCGGACCCCG AACTAGCATC TCAGGAGCAG AGGCTGGATT ACCATGATGA CGACCAGCGG
    CTCCGCAGAG AGGAGTTTGA GGAGAACCTG CGTGAAATGG GCCTTGAGAT GGAAAAAGAG
    GAAGGGAATA AAATTCCTGG CGTGGGGTTC TTGAAGATCC ACGCTCCTTG GGAAGTTCTT
    TGTCGAGAAG CAGAGTTTAT GAAACTAAAA ATGCCCACAA TAAAGAAATA TGAAGTAAGC
    CATGGAAACA GCATTGTGGA GAAGGCCAGC AGGTTTATGC AGAAATTCAC AGCACCTTTG
    CAACCTAAGG TTGGGGACCG TCAGATTGAA CACGAGAAAC ACCTCTCATA CCCATTCTCA
    CGAGAAAAGC AGCATCTCTT TGATTTATCA GACAGGAATT CCTTCTTCGA CAGCAAAACC
    AGGAGCTCAA TAGTGTATGA GATTTTAAAG CGAACAAAAT GCACACGGGC GAAGTATATC
    ATGGGAATCA TCAGTCTTCT GGGCAACGGT ATCTACACTT CAGCCTACCC TTTGCATGAT
    GGCGATATTG ATGACGTCCA TGCTGAAATA AATGACAGAA AGATCTTATA TGAAGAGTGG
    GCGAGTTACA GCATATTCTA CAAGTATCAA CCCATCGGAC TGATCAGGAA GTATTTTGGT
    GAGAAAGTGG GCCTGTACTT CGCCTGGCTG GGTGTGTACA CTCAGATGCT CATCCCAGCA
    GCCATTGTGG GCGTGATCGT CTTCCTGTAC GGCTGCGCTA CTGTGGACGA CAACATTCCC
    AGTATGGAGA TCTGCGACGA GAGAAACAAC ATCACCATGT GCCCAATGTG CGACCGGGCC
    TGCAGCTACT GGAAACTGGT GACGGCTTGC GGCACAGCCA GAGCCAGCCA CCTGTTCGAC
    AACGCGGCCA CCGTCTTCTT TGCCATCTTC ATGGCACTAT GGGCCGCCAT GTTTATGGAG
    CACTGGAAGA GACGGCAAGT GAGGCTCAAC TACCTCTGGG ACCTGACGGG TTTTGAGGAC
    ACCGAGGGGA AACAAAAGAG TCATCCGAGA GCGGAGTATG AGTTTCATGT CATGAAGAAG
    TCATTGAGGA AAGAGAAATC TCCAAAGGCA GGGAATGTGA AGCTGACATG CACCGACAGA
    ATGCCAGCTT ACATGACCAT CATCATCACC ATGATTTTTC TGATCTGTGT GACCTTGGCC
    ATCGTGTTTG GTGTGGTGCT ATACAGGGTG TCTATTATGA CAGCCCTTCA TATGAGTTCC
    ACTCCTACAT TCCGTTCTAA TATACGTGCC ACCGTTAAAA CCACCGGCGC CATCATCAAC
    CTCATTATCA TCATCATCAT GGATGAGGTC TACGGAGCAG TTGCACGTTG GCTCACTATT
    ATGGAGGTGC CAAAGACTGA CAAGAGTTTT GAGGAGAGGC TGATCTTCAA GACATTCGTA
    TTGAAGTTTG CGAATGCATT TACTCCTATT GTGTACCTGG CGTTCTTCAG AGGCAGACTC
    ATTGGACGTC CTGGCAAATA TATCTATGTT ATGGGATCTT ACAGAATGGA AGAGTGCGCT
    CATGCCGGCT GTCTGATGGA ACTGTGCATT CAGCTCTGCA TCACTATGCT TGGCAAACAG
    CTCATCCAGA ATAACCTGTT TGAGATCGGC GTTCCGAAAA TCAAGAAGCT GCTCCGACAA
    CGCAAGATTG ACCCAAAGCA CCAGGAAGAG CTGAACAAAA CGCTTCATCG CCATGAGAAG
    GACCACTTCC TAGGACCCTT TGTCGGACTC AACCCAGAGT ACATGGAAAT GATCATTCAG
    TTCGGGATGG TGACCTTATT CGTGGCCTCT TTTCCGCTGG CTCCTCTCTT TGCTCTGCTC
    AACAACATCA TTGAGATCCG TCTTGATGCC AAAAAGTTTG TTACGGAACT GAGAAGGCCA
    ATCGCTGTAA GAGCAAAGGA CATCGGAATC TGGTACACTC TTCTCAGAGG ACTCAGCAAA
    GTGGCTGTCA TTGTCAATGC GTTTGTTATC TCGTTCACCT CTGACTTCAT CCCGCGGCTG
    GTCTATCAAT ACATGTACAG TCCAGACGGG ACCCTCCATG GCTTTGTCAA TCACACGCTC
    TCGTATTTTA ACGTCACTCA CTTCCAGCCT GGCACAGAGC CCCTGGAGCC CATGCACCTG
    GGATACCAAG TGGACACGTG CAGATATAAA GATTACAGGG ATCCTCCATG GTCTGATACA
    CCGTACGAGT TCTCAAGAGA ATTCTGGGCC ATTCTGGCAG CCCGCTTCGC TTTCGTAATT
    CTCTTTCAGA ATGTGGTGAT GCTGATGAGT GACTTCGTGG ACTGGCTCAT CCCAGACATC
    CCCAAAGACA TCAGCTTGAA AATCCACAAG GAGAGGAACC TCATCATTGA GCTGTTCATG
    AAGCGGGAAC AGGGAAAGAA GTACAGGAAC ACCATGAACG ACCCAAGTCC ACAACCGCTC
    CGCTCGCGGC CCAGTTCACA AGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13473
    Clone ID Related Accession (Same CDS sequence) XM_009303473.3 , XM_009303473.2
    Accession Version XM_009303473.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2787bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product anoctamin-1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303473.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTGGCGAA ATGAAAAGTA TGAATCTGTC CCGTTGGATG TGAAGGGGAT TGATGAAGAC 
    AATTCTAAAG GCACAAGGGA TTCTTTATAT CCCCACACTA CTCTCCTGAA AAGTCAGCAG
    GCGGATGAGA AACCAAAGGA CCCAAAGCTT TGGCCGTACT TCAAGGATGG TCACAGACGG
    ATAGATTATG TGCTCACCTA CCACATCCAG AAGCCCCAGA ACACTGGCAA ACAGTCATCC
    AAGTCCTGCC TGGGGGGTTG TTTGTGCTGC AAAAAACGAG GCCAGACAAA CCCACCGGTT
    CCGGACCCCG AACTAGCATC TCAGGAGCAG AGGCTGGATT ACCATGATGA CGACCAGCGG
    CTCCGCAGAG AGGAGTTTGA GGAGAACCTG CGTGAAATGG GCCTTGAGAT GGAAAAAGAG
    GAAGGGAATA AAATTCCTGG CGTGGGGTTC TTGAAGATCC ACGCTCCTTG GGAAGTTCTT
    TGTCGAGAAG CAGAGTTTAT GAAACTAAAA ATGCCCACAA TAAAGAAATA TGAAGTAAGC
    CATGGAAACA GCATTGTGGA GAAGGCCAGC AGGTTTATGC AGAAATTCAC AGCACCTTTG
    CAACCTAAGG TTGGGGACCG TCAGATTGAA CACGAGAAAC ACCTCTCATA CCCATTCTCA
    CGAGAAAAGC AGCATCTCTT TGATTTATCA GACAGGAATT CCTTCTTCGA CAGCAAAACC
    AGGAGCTCAA TAGTGTATGA GATTTTAAAG CGAACAAAAT GCACACGGGC GAAGTATATC
    ATGGGAATCA TCAGTCTTCT GGGCAACGGT ATCTACACTT CAGCCTACCC TTTGCATGAT
    GGCGATATTG ATGACGTCCA TGCTGAAATA AATGACAGAA AGATCTTATA TGAAGAGTGG
    GCGAGTTACA GCATATTCTA CAAGTATCAA CCCATCGGAC TGATCAGGAA GTATTTTGGT
    GAGAAAGTGG GCCTGTACTT CGCCTGGCTG GGTGTGTACA CTCAGATGCT CATCCCAGCA
    GCCATTGTGG GCGTGATCGT CTTCCTGTAC GGCTGCGCTA CTGTGGACGA CAACATTCCC
    AGTATGGAGA TCTGCGACGA GAGAAACAAC ATCACCATGT GCCCAATGTG CGACCGGGCC
    TGCAGCTACT GGAAACTGGT GACGGCTTGC GGCACAGCCA GAGCCAGCCA CCTGTTCGAC
    AACGCGGCCA CCGTCTTCTT TGCCATCTTC ATGGCACTAT GGGCCGCCAT GTTTATGGAG
    CACTGGAAGA GACGGCAAGT GAGGCTCAAC TACCTCTGGG ACCTGACGGG TTTTGAGGAC
    ACCGAGGGGA AACAAAAGAG TCATCCGAGA GCGGAGTATG AGTTTCATGT CATGAAGAAG
    TCATTGAGGA AAGAGAAATC TCCAAAGGCA GGGAATGTGA AGCTGACATG CACCGACAGA
    ATGCCAGCTT ACATGACCAT CATCATCACC ATGATTTTTC TGATCTGTGT GACCTTGGCC
    ATCGTGTTTG GTGTGGTGCT ATACAGGGTG TCTATTATGA CAGCCCTTCA TATGAGTTCC
    ACTCCTACAT TCCGTTCTAA TATACGTGCC ACCGTTAAAA CCACCGGCGC CATCATCAAC
    CTCATTATCA TCATCATCAT GGATGAGGTC TACGGAGCAG TTGCACGTTG GCTCACTATT
    ATGGAGGTGC CAAAGACTGA CAAGAGTTTT GAGGAGAGGC TGATCTTCAA GACATTCGTA
    TTGAAGTTTG CGAATGCATT TACTCCTATT GTGTACCTGG CGTTCTTCAG AGGCAGACTC
    ATTGGACGTC CTGGCAAATA TATCTATGTT ATGGGATCTT ACAGAATGGA AGAGTGCGCT
    CATGCCGGCT GTCTGATGGA ACTGTGCATT CAGCTCTGCA TCACTATGCT TGGCAAACAG
    CTCATCCAGA ATAACCTGTT TGAGATCGGC GTTCCGAAAA TCAAGAAGCT GCTCCGACAA
    CGCAAGATTG ACCCAAAGCA CCAGGAAGAG CTGAACAAAA CGCTTCATCG CCATGAGAAG
    GACCACTTCC TAGGACCCTT TGTCGGACTC AACCCAGAGT ACATGGAAAT GATCATTCAG
    TTCGGGATGG TGACCTTATT CGTGGCCTCT TTTCCGCTGG CTCCTCTCTT TGCTCTGCTC
    AACAACATCA TTGAGATCCG TCTTGATGCC AAAAAGTTTG TTACGGAACT GAGAAGGCCA
    ATCGCTGTAA GAGCAAAGGA CATCGGAATC TGGTACACTC TTCTCAGAGG ACTCAGCAAA
    GTGGCTGTCA TTGTCAATGC GTTTGTTATC TCGTTCACCT CTGACTTCAT CCCGCGGCTG
    GTCTATCAAT ACATGTACAG TCCAGACGGG ACCCTCCATG GCTTTGTCAA TCACACGCTC
    TCGTATTTTA ACGTCACTCA CTTCCAGCCT GGCACAGAGC CCCTGGAGCC CATGCACCTG
    GGATACCAAG TGGACACGTG CAGATATAAA GATTACAGGG ATCCTCCATG GTCTGATACA
    CCGTACGAGT TCTCAAGAGA ATTCTGGGCC ATTCTGGCAG CCCGCTTCGC TTTCGTAATT
    CTCTTTCAGA ATGTGGTGAT GCTGATGAGT GACTTCGTGG ACTGGCTCAT CCCAGACATC
    CCCAAAGACA TCAGCTTGAA AATCCACAAG GAGAGGAACC TCATCATTGA GCTGTTCATG
    AAGCGGGAAC AGGGAAAGAA GTACAGGAAC ACCATGAACG ACCCAAGTCC ACAACCGCTC
    CGCTCGCGGC CCAGTTCACA AGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301748.1
    CDS299..3085
    Translation

    Target ORF information:

    RefSeq Version XM_009303473.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio anoctamin 1, calcium activated chloride channel (ano1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303473.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTGGCGAA ATGAAAAGTA TGAATCTGTC CCGTTGGATG TGAAGGGGAT TGATGAAGAC 
    AATTCTAAAG GCACAAGGGA TTCTTTATAT CCCCACACTA CTCTCCTGAA AAGTCAGCAG
    GCGGATGAGA AACCAAAGGA CCCAAAGCTT TGGCCGTACT TCAAGGATGG TCACAGACGG
    ATAGATTATG TGCTCACCTA CCACATCCAG AAGCCCCAGA ACACTGGCAA ACAGTCATCC
    AAGTCCTGCC TGGGGGGTTG TTTGTGCTGC AAAAAACGAG GCCAGACAAA CCCACCGGTT
    CCGGACCCCG AACTAGCATC TCAGGAGCAG AGGCTGGATT ACCATGATGA CGACCAGCGG
    CTCCGCAGAG AGGAGTTTGA GGAGAACCTG CGTGAAATGG GCCTTGAGAT GGAAAAAGAG
    GAAGGGAATA AAATTCCTGG CGTGGGGTTC TTGAAGATCC ACGCTCCTTG GGAAGTTCTT
    TGTCGAGAAG CAGAGTTTAT GAAACTAAAA ATGCCCACAA TAAAGAAATA TGAAGTAAGC
    CATGGAAACA GCATTGTGGA GAAGGCCAGC AGGTTTATGC AGAAATTCAC AGCACCTTTG
    CAACCTAAGG TTGGGGACCG TCAGATTGAA CACGAGAAAC ACCTCTCATA CCCATTCTCA
    CGAGAAAAGC AGCATCTCTT TGATTTATCA GACAGGAATT CCTTCTTCGA CAGCAAAACC
    AGGAGCTCAA TAGTGTATGA GATTTTAAAG CGAACAAAAT GCACACGGGC GAAGTATATC
    ATGGGAATCA TCAGTCTTCT GGGCAACGGT ATCTACACTT CAGCCTACCC TTTGCATGAT
    GGCGATATTG ATGACGTCCA TGCTGAAATA AATGACAGAA AGATCTTATA TGAAGAGTGG
    GCGAGTTACA GCATATTCTA CAAGTATCAA CCCATCGGAC TGATCAGGAA GTATTTTGGT
    GAGAAAGTGG GCCTGTACTT CGCCTGGCTG GGTGTGTACA CTCAGATGCT CATCCCAGCA
    GCCATTGTGG GCGTGATCGT CTTCCTGTAC GGCTGCGCTA CTGTGGACGA CAACATTCCC
    AGTATGGAGA TCTGCGACGA GAGAAACAAC ATCACCATGT GCCCAATGTG CGACCGGGCC
    TGCAGCTACT GGAAACTGGT GACGGCTTGC GGCACAGCCA GAGCCAGCCA CCTGTTCGAC
    AACGCGGCCA CCGTCTTCTT TGCCATCTTC ATGGCACTAT GGGCCGCCAT GTTTATGGAG
    CACTGGAAGA GACGGCAAGT GAGGCTCAAC TACCTCTGGG ACCTGACGGG TTTTGAGGAC
    ACCGAGGGGA AACAAAAGAG TCATCCGAGA GCGGAGTATG AGTTTCATGT CATGAAGAAG
    TCATTGAGGA AAGAGAAATC TCCAAAGGCA GGGAATGTGA AGCTGACATG CACCGACAGA
    ATGCCAGCTT ACATGACCAT CATCATCACC ATGATTTTTC TGATCTGTGT GACCTTGGCC
    ATCGTGTTTG GTGTGGTGCT ATACAGGGTG TCTATTATGA CAGCCCTTCA TATGAGTTCC
    ACTCCTACAT TCCGTTCTAA TATACGTGCC ACCGTTAAAA CCACCGGCGC CATCATCAAC
    CTCATTATCA TCATCATCAT GGATGAGGTC TACGGAGCAG TTGCACGTTG GCTCACTATT
    ATGGAGGTGC CAAAGACTGA CAAGAGTTTT GAGGAGAGGC TGATCTTCAA GACATTCGTA
    TTGAAGTTTG CGAATGCATT TACTCCTATT GTGTACCTGG CGTTCTTCAG AGGCAGACTC
    ATTGGACGTC CTGGCAAATA TATCTATGTT ATGGGATCTT ACAGAATGGA AGAGTGCGCT
    CATGCCGGCT GTCTGATGGA ACTGTGCATT CAGCTCTGCA TCACTATGCT TGGCAAACAG
    CTCATCCAGA ATAACCTGTT TGAGATCGGC GTTCCGAAAA TCAAGAAGCT GCTCCGACAA
    CGCAAGATTG ACCCAAAGCA CCAGGAAGAG CTGAACAAAA CGCTTCATCG CCATGAGAAG
    GACCACTTCC TAGGACCCTT TGTCGGACTC AACCCAGAGT ACATGGAAAT GATCATTCAG
    TTCGGGATGG TGACCTTATT CGTGGCCTCT TTTCCGCTGG CTCCTCTCTT TGCTCTGCTC
    AACAACATCA TTGAGATCCG TCTTGATGCC AAAAAGTTTG TTACGGAACT GAGAAGGCCA
    ATCGCTGTAA GAGCAAAGGA CATCGGAATC TGGTACACTC TTCTCAGAGG ACTCAGCAAA
    GTGGCTGTCA TTGTCAATGC GTTTGTTATC TCGTTCACCT CTGACTTCAT CCCGCGGCTG
    GTCTATCAAT ACATGTACAG TCCAGACGGG ACCCTCCATG GCTTTGTCAA TCACACGCTC
    TCGTATTTTA ACGTCACTCA CTTCCAGCCT GGCACAGAGC CCCTGGAGCC CATGCACCTG
    GGATACCAAG TGGACACGTG CAGATATAAA GATTACAGGG ATCCTCCATG GTCTGATACA
    CCGTACGAGT TCTCAAGAGA ATTCTGGGCC ATTCTGGCAG CCCGCTTCGC TTTCGTAATT
    CTCTTTCAGA ATGTGGTGAT GCTGATGAGT GACTTCGTGG ACTGGCTCAT CCCAGACATC
    CCCAAAGACA TCAGCTTGAA AATCCACAAG GAGAGGAACC TCATCATTGA GCTGTTCATG
    AAGCGGGAAC AGGGAAAGAA GTACAGGAAC ACCATGAACG ACCCAAGTCC ACAACCGCTC
    CGCTCGCGGC CCAGTTCACA AGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa40966
    Clone ID Related Accession (Same CDS sequence) XM_009303472.3 , XM_009303472.2
    Accession Version XM_009303472.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product anoctamin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303472.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAGGGTG AAGATGATGT AAATACTGCG AGGAAAGATG ATGAGGCTGA CGGCTCGGGT 
    GAGAAACTCT TGGGTTTCAA CAGCACAGAG GAGCAGCCGT CAGGACAGGA GAGCATGTGG
    CGAAATGAAA AGTATGAATC TGTCCCGTTG GATGTGAAGG GGATTGATGA AGACAATTCT
    AAAGGCACAA GGGATTCTTT ATATCCCCAC ACTACTCTCC TGAAAAGTCA GCAGGCGGAT
    GAGAAACCAA AGGACCCAAA GCTTTGGCCG TACTTCAAGG ATGGTCACAG ACGGATAGAT
    TATGTGCTCA CCTACCACAT CCAGAAGCCC CAGAACACTG GCAAACAGTC ATCCAAGTCC
    TGCCTGGGGG GTTGTTTGTG CTGCAAAAAA CGAGGCCAGA CAAACCCACC GGTTCCGGAC
    CCCGAACTAG CATCTCAGGA GCAGAGGCTG GATTACCATG ATGACGACCA GCGGCTCCGC
    AGAGAGGAGT TTGAGGAGAA CCTGCGTGAA ATGGGCCTTG AGATGGAAAA AGAGGAAGGG
    AATAAAATTC CTGGCGTGGG GTTCTTGAAG ATCCACGCTC CTTGGGAAGT TCTTTGTCGA
    GAAGCAGAGT TTATGAAACT AAAAATGCCC ACAATAAAGA AATATGAAGT AAGCCATGGA
    AACAGCATTG TGGAGAAGGC CAGCAGGTTT ATGCAGAAAT TCACAGCACC TTTGCAACCT
    AAGGTTGGGG ACCGTCAGAT TGAACACGAG AAACACCTCT CATACCCATT CTCACGAGAA
    AAGCAGCATC TCTTTGATTT ATCAGACAGG AATTCCTTCT TCGACAGCAA AACCAGGAGC
    TCAATAGTGT ATGAGATTTT AAAGCGAACA AAATGCACAC GGGCGAAGTA TATCATGGGA
    ATCATCAGTC TTCTGGGCAA CGGTATCTAC ACTTCAGCCT ACCCTTTGCA TGATGGCGAT
    ATTGATGACG TCCATGCTGA AATAAATGAC AGAAAGATCT TATATGAAGA GTGGGCGAGT
    TACAGCATAT TCTACAAGTA TCAACCCATC GGACTGATCA GGAAGTATTT TGGTGAGAAA
    GTGGGCCTGT ACTTCGCCTG GCTGGGTGTG TACACTCAGA TGCTCATCCC AGCAGCCATT
    GTGGGCGTGA TCGTCTTCCT GTACGGCTGC GCTACTGTGG ACGACAACAT TCCCAGTATG
    GAGATCTGCG ACGAGAGAAA CAACATCACC ATGTGCCCAA TGTGCGACCG GGCCTGCAGC
    TACTGGAAAC TGGTGACGGC TTGCGGCACA GCCAGAGCCA GCCACCTGTT CGACAACGCG
    GCCACCGTCT TCTTTGCCAT CTTCATGGCA CTATGGGCCG CCATGTTTAT GGAGCACTGG
    AAGAGACGGC AAGTGAGGCT CAACTACCTC TGGGACCTGA CGGGTTTTGA GGACACCGAG
    AGTCATCCGA GAGCGGAGTA TGAGTTTCAT GTCATGAAGA AGTCATTGAG GAAAGAGAAA
    TCTCCAAAGG CAGGGAATGT GAAGCTGACA TGCACCGACA GAATGCCAGC TTACATGACC
    ATCATCATCA CCATGATTTT TCTGATCTGT GTGACCTTGG CCATCGTGTT TGGTGTGGTG
    CTATACAGGG TGTCTATTAT GACAGCCCTT CATATGAGTT CCACTCCTAC ATTCCGTTCT
    AATATACGTG CCACCGTTAA AACCACCGGC GCCATCATCA ACCTCATTAT CATCATCATC
    ATGGATGAGG TCTACGGAGC AGTTGCACGT TGGCTCACTA TTATGGAGGT GCCAAAGACT
    GACAAGAGTT TTGAGGAGAG GCTGATCTTC AAGACATTCG TATTGAAGTT TGCGAATGCA
    TTTACTCCTA TTGTGTACCT GGCGTTCTTC AGAGGCAGAC TCATTGGACG TCCTGGCAAA
    TATATCTATG TTATGGGATC TTACAGAATG GAAGAGTGCG CTCATGCCGG CTGTCTGATG
    GAACTGTGCA TTCAGCTCTG CATCACTATG CTTGGCAAAC AGCTCATCCA GAATAACCTG
    TTTGAGATCG GCGTTCCGAA AATCAAGAAG CTGCTCCGAC AACGCAAGAT TGACCCAAAG
    CACCAGGAAG AGCTGAACAA AACGCTTCAT CGCCATGAGA AGGACCACTT CCTAGGACCC
    TTTGTCGGAC TCAACCCAGA GTACATGGAA ATGATCATTC AGTTCGGGAT GGTGACCTTA
    TTCGTGGCCT CTTTTCCGCT GGCTCCTCTC TTTGCTCTGC TCAACAACAT CATTGAGATC
    CGTCTTGATG CCAAAAAGTT TGTTACGGAA CTGAGAAGGC CAATCGCTGT AAGAGCAAAG
    GACATCGGAA TCTGGTACAC TCTTCTCAGA GGACTCAGCA AAGTGGCTGT CATTGTCAAT
    GCGTTTGTTA TCTCGTTCAC CTCTGACTTC ATCCCGCGGC TGGTCTATCA ATACATGTAC
    AGTCCAGACG GGACCCTCCA TGGCTTTGTC AATCACACGC TCTCGTATTT TAACGTCACT
    CACTTCCAGC CTGGCACAGA GCCCCTGGAG CCCATGCACC TGGGATACCA AGTGGACACG
    TGCAGATATA AAGATTACAG GGATCCTCCA TGGTCTGATA CACCGTACGA GTTCTCAAGA
    GAATTCTGGG CCATTCTGGC AGCCCGCTTC GCTTTCGTAA TTCTCTTTCA GAATGTGGTG
    ATGCTGATGA GTGACTTCGT GGACTGGCTC ATCCCAGACA TCCCCAAAGA CATCAGCTTG
    AAAATCCACA AGGAGAGGAA CCTCATCATT GAGCTGTTCA TGAAGCGGGA ACAGGGAAAG
    AAGTACAGGA ACACCATGAA CGACCCAAGT CCACAACCGC TCCGCTCGCG GCCCAGTTCA
    CAAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301747.1
    CDS208..3096
    Translation

    Target ORF information:

    RefSeq Version XM_009303472.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio anoctamin 1, calcium activated chloride channel (ano1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303472.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAGGGTG AAGATGATGT AAATACTGCG AGGAAAGATG ATGAGGCTGA CGGCTCGGGT 
    GAGAAACTCT TGGGTTTCAA CAGCACAGAG GAGCAGCCGT CAGGACAGGA GAGCATGTGG
    CGAAATGAAA AGTATGAATC TGTCCCGTTG GATGTGAAGG GGATTGATGA AGACAATTCT
    AAAGGCACAA GGGATTCTTT ATATCCCCAC ACTACTCTCC TGAAAAGTCA GCAGGCGGAT
    GAGAAACCAA AGGACCCAAA GCTTTGGCCG TACTTCAAGG ATGGTCACAG ACGGATAGAT
    TATGTGCTCA CCTACCACAT CCAGAAGCCC CAGAACACTG GCAAACAGTC ATCCAAGTCC
    TGCCTGGGGG GTTGTTTGTG CTGCAAAAAA CGAGGCCAGA CAAACCCACC GGTTCCGGAC
    CCCGAACTAG CATCTCAGGA GCAGAGGCTG GATTACCATG ATGACGACCA GCGGCTCCGC
    AGAGAGGAGT TTGAGGAGAA CCTGCGTGAA ATGGGCCTTG AGATGGAAAA AGAGGAAGGG
    AATAAAATTC CTGGCGTGGG GTTCTTGAAG ATCCACGCTC CTTGGGAAGT TCTTTGTCGA
    GAAGCAGAGT TTATGAAACT AAAAATGCCC ACAATAAAGA AATATGAAGT AAGCCATGGA
    AACAGCATTG TGGAGAAGGC CAGCAGGTTT ATGCAGAAAT TCACAGCACC TTTGCAACCT
    AAGGTTGGGG ACCGTCAGAT TGAACACGAG AAACACCTCT CATACCCATT CTCACGAGAA
    AAGCAGCATC TCTTTGATTT ATCAGACAGG AATTCCTTCT TCGACAGCAA AACCAGGAGC
    TCAATAGTGT ATGAGATTTT AAAGCGAACA AAATGCACAC GGGCGAAGTA TATCATGGGA
    ATCATCAGTC TTCTGGGCAA CGGTATCTAC ACTTCAGCCT ACCCTTTGCA TGATGGCGAT
    ATTGATGACG TCCATGCTGA AATAAATGAC AGAAAGATCT TATATGAAGA GTGGGCGAGT
    TACAGCATAT TCTACAAGTA TCAACCCATC GGACTGATCA GGAAGTATTT TGGTGAGAAA
    GTGGGCCTGT ACTTCGCCTG GCTGGGTGTG TACACTCAGA TGCTCATCCC AGCAGCCATT
    GTGGGCGTGA TCGTCTTCCT GTACGGCTGC GCTACTGTGG ACGACAACAT TCCCAGTATG
    GAGATCTGCG ACGAGAGAAA CAACATCACC ATGTGCCCAA TGTGCGACCG GGCCTGCAGC
    TACTGGAAAC TGGTGACGGC TTGCGGCACA GCCAGAGCCA GCCACCTGTT CGACAACGCG
    GCCACCGTCT TCTTTGCCAT CTTCATGGCA CTATGGGCCG CCATGTTTAT GGAGCACTGG
    AAGAGACGGC AAGTGAGGCT CAACTACCTC TGGGACCTGA CGGGTTTTGA GGACACCGAG
    AGTCATCCGA GAGCGGAGTA TGAGTTTCAT GTCATGAAGA AGTCATTGAG GAAAGAGAAA
    TCTCCAAAGG CAGGGAATGT GAAGCTGACA TGCACCGACA GAATGCCAGC TTACATGACC
    ATCATCATCA CCATGATTTT TCTGATCTGT GTGACCTTGG CCATCGTGTT TGGTGTGGTG
    CTATACAGGG TGTCTATTAT GACAGCCCTT CATATGAGTT CCACTCCTAC ATTCCGTTCT
    AATATACGTG CCACCGTTAA AACCACCGGC GCCATCATCA ACCTCATTAT CATCATCATC
    ATGGATGAGG TCTACGGAGC AGTTGCACGT TGGCTCACTA TTATGGAGGT GCCAAAGACT
    GACAAGAGTT TTGAGGAGAG GCTGATCTTC AAGACATTCG TATTGAAGTT TGCGAATGCA
    TTTACTCCTA TTGTGTACCT GGCGTTCTTC AGAGGCAGAC TCATTGGACG TCCTGGCAAA
    TATATCTATG TTATGGGATC TTACAGAATG GAAGAGTGCG CTCATGCCGG CTGTCTGATG
    GAACTGTGCA TTCAGCTCTG CATCACTATG CTTGGCAAAC AGCTCATCCA GAATAACCTG
    TTTGAGATCG GCGTTCCGAA AATCAAGAAG CTGCTCCGAC AACGCAAGAT TGACCCAAAG
    CACCAGGAAG AGCTGAACAA AACGCTTCAT CGCCATGAGA AGGACCACTT CCTAGGACCC
    TTTGTCGGAC TCAACCCAGA GTACATGGAA ATGATCATTC AGTTCGGGAT GGTGACCTTA
    TTCGTGGCCT CTTTTCCGCT GGCTCCTCTC TTTGCTCTGC TCAACAACAT CATTGAGATC
    CGTCTTGATG CCAAAAAGTT TGTTACGGAA CTGAGAAGGC CAATCGCTGT AAGAGCAAAG
    GACATCGGAA TCTGGTACAC TCTTCTCAGA GGACTCAGCA AAGTGGCTGT CATTGTCAAT
    GCGTTTGTTA TCTCGTTCAC CTCTGACTTC ATCCCGCGGC TGGTCTATCA ATACATGTAC
    AGTCCAGACG GGACCCTCCA TGGCTTTGTC AATCACACGC TCTCGTATTT TAACGTCACT
    CACTTCCAGC CTGGCACAGA GCCCCTGGAG CCCATGCACC TGGGATACCA AGTGGACACG
    TGCAGATATA AAGATTACAG GGATCCTCCA TGGTCTGATA CACCGTACGA GTTCTCAAGA
    GAATTCTGGG CCATTCTGGC AGCCCGCTTC GCTTTCGTAA TTCTCTTTCA GAATGTGGTG
    ATGCTGATGA GTGACTTCGT GGACTGGCTC ATCCCAGACA TCCCCAAAGA CATCAGCTTG
    AAAATCCACA AGGAGAGGAA CCTCATCATT GAGCTGTTCA TGAAGCGGGA ACAGGGAAAG
    AAGTACAGGA ACACCATGAA CGACCCAAGT CCACAACCGC TCCGCTCGCG GCCCAGTTCA
    CAAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa40966
    Clone ID Related Accession (Same CDS sequence) XM_009303472.3 , XM_009303472.2
    Accession Version XM_009303472.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product anoctamin-1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303472.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAGGGTG AAGATGATGT AAATACTGCG AGGAAAGATG ATGAGGCTGA CGGCTCGGGT 
    GAGAAACTCT TGGGTTTCAA CAGCACAGAG GAGCAGCCGT CAGGACAGGA GAGCATGTGG
    CGAAATGAAA AGTATGAATC TGTCCCGTTG GATGTGAAGG GGATTGATGA AGACAATTCT
    AAAGGCACAA GGGATTCTTT ATATCCCCAC ACTACTCTCC TGAAAAGTCA GCAGGCGGAT
    GAGAAACCAA AGGACCCAAA GCTTTGGCCG TACTTCAAGG ATGGTCACAG ACGGATAGAT
    TATGTGCTCA CCTACCACAT CCAGAAGCCC CAGAACACTG GCAAACAGTC ATCCAAGTCC
    TGCCTGGGGG GTTGTTTGTG CTGCAAAAAA CGAGGCCAGA CAAACCCACC GGTTCCGGAC
    CCCGAACTAG CATCTCAGGA GCAGAGGCTG GATTACCATG ATGACGACCA GCGGCTCCGC
    AGAGAGGAGT TTGAGGAGAA CCTGCGTGAA ATGGGCCTTG AGATGGAAAA AGAGGAAGGG
    AATAAAATTC CTGGCGTGGG GTTCTTGAAG ATCCACGCTC CTTGGGAAGT TCTTTGTCGA
    GAAGCAGAGT TTATGAAACT AAAAATGCCC ACAATAAAGA AATATGAAGT AAGCCATGGA
    AACAGCATTG TGGAGAAGGC CAGCAGGTTT ATGCAGAAAT TCACAGCACC TTTGCAACCT
    AAGGTTGGGG ACCGTCAGAT TGAACACGAG AAACACCTCT CATACCCATT CTCACGAGAA
    AAGCAGCATC TCTTTGATTT ATCAGACAGG AATTCCTTCT TCGACAGCAA AACCAGGAGC
    TCAATAGTGT ATGAGATTTT AAAGCGAACA AAATGCACAC GGGCGAAGTA TATCATGGGA
    ATCATCAGTC TTCTGGGCAA CGGTATCTAC ACTTCAGCCT ACCCTTTGCA TGATGGCGAT
    ATTGATGACG TCCATGCTGA AATAAATGAC AGAAAGATCT TATATGAAGA GTGGGCGAGT
    TACAGCATAT TCTACAAGTA TCAACCCATC GGACTGATCA GGAAGTATTT TGGTGAGAAA
    GTGGGCCTGT ACTTCGCCTG GCTGGGTGTG TACACTCAGA TGCTCATCCC AGCAGCCATT
    GTGGGCGTGA TCGTCTTCCT GTACGGCTGC GCTACTGTGG ACGACAACAT TCCCAGTATG
    GAGATCTGCG ACGAGAGAAA CAACATCACC ATGTGCCCAA TGTGCGACCG GGCCTGCAGC
    TACTGGAAAC TGGTGACGGC TTGCGGCACA GCCAGAGCCA GCCACCTGTT CGACAACGCG
    GCCACCGTCT TCTTTGCCAT CTTCATGGCA CTATGGGCCG CCATGTTTAT GGAGCACTGG
    AAGAGACGGC AAGTGAGGCT CAACTACCTC TGGGACCTGA CGGGTTTTGA GGACACCGAG
    AGTCATCCGA GAGCGGAGTA TGAGTTTCAT GTCATGAAGA AGTCATTGAG GAAAGAGAAA
    TCTCCAAAGG CAGGGAATGT GAAGCTGACA TGCACCGACA GAATGCCAGC TTACATGACC
    ATCATCATCA CCATGATTTT TCTGATCTGT GTGACCTTGG CCATCGTGTT TGGTGTGGTG
    CTATACAGGG TGTCTATTAT GACAGCCCTT CATATGAGTT CCACTCCTAC ATTCCGTTCT
    AATATACGTG CCACCGTTAA AACCACCGGC GCCATCATCA ACCTCATTAT CATCATCATC
    ATGGATGAGG TCTACGGAGC AGTTGCACGT TGGCTCACTA TTATGGAGGT GCCAAAGACT
    GACAAGAGTT TTGAGGAGAG GCTGATCTTC AAGACATTCG TATTGAAGTT TGCGAATGCA
    TTTACTCCTA TTGTGTACCT GGCGTTCTTC AGAGGCAGAC TCATTGGACG TCCTGGCAAA
    TATATCTATG TTATGGGATC TTACAGAATG GAAGAGTGCG CTCATGCCGG CTGTCTGATG
    GAACTGTGCA TTCAGCTCTG CATCACTATG CTTGGCAAAC AGCTCATCCA GAATAACCTG
    TTTGAGATCG GCGTTCCGAA AATCAAGAAG CTGCTCCGAC AACGCAAGAT TGACCCAAAG
    CACCAGGAAG AGCTGAACAA AACGCTTCAT CGCCATGAGA AGGACCACTT CCTAGGACCC
    TTTGTCGGAC TCAACCCAGA GTACATGGAA ATGATCATTC AGTTCGGGAT GGTGACCTTA
    TTCGTGGCCT CTTTTCCGCT GGCTCCTCTC TTTGCTCTGC TCAACAACAT CATTGAGATC
    CGTCTTGATG CCAAAAAGTT TGTTACGGAA CTGAGAAGGC CAATCGCTGT AAGAGCAAAG
    GACATCGGAA TCTGGTACAC TCTTCTCAGA GGACTCAGCA AAGTGGCTGT CATTGTCAAT
    GCGTTTGTTA TCTCGTTCAC CTCTGACTTC ATCCCGCGGC TGGTCTATCA ATACATGTAC
    AGTCCAGACG GGACCCTCCA TGGCTTTGTC AATCACACGC TCTCGTATTT TAACGTCACT
    CACTTCCAGC CTGGCACAGA GCCCCTGGAG CCCATGCACC TGGGATACCA AGTGGACACG
    TGCAGATATA AAGATTACAG GGATCCTCCA TGGTCTGATA CACCGTACGA GTTCTCAAGA
    GAATTCTGGG CCATTCTGGC AGCCCGCTTC GCTTTCGTAA TTCTCTTTCA GAATGTGGTG
    ATGCTGATGA GTGACTTCGT GGACTGGCTC ATCCCAGACA TCCCCAAAGA CATCAGCTTG
    AAAATCCACA AGGAGAGGAA CCTCATCATT GAGCTGTTCA TGAAGCGGGA ACAGGGAAAG
    AAGTACAGGA ACACCATGAA CGACCCAAGT CCACAACCGC TCCGCTCGCG GCCCAGTTCA
    CAAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301747.1
    CDS199..3087
    Translation

    Target ORF information:

    RefSeq Version XM_009303472.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio anoctamin 1, calcium activated chloride channel (ano1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303472.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAGGGTG AAGATGATGT AAATACTGCG AGGAAAGATG ATGAGGCTGA CGGCTCGGGT 
    GAGAAACTCT TGGGTTTCAA CAGCACAGAG GAGCAGCCGT CAGGACAGGA GAGCATGTGG
    CGAAATGAAA AGTATGAATC TGTCCCGTTG GATGTGAAGG GGATTGATGA AGACAATTCT
    AAAGGCACAA GGGATTCTTT ATATCCCCAC ACTACTCTCC TGAAAAGTCA GCAGGCGGAT
    GAGAAACCAA AGGACCCAAA GCTTTGGCCG TACTTCAAGG ATGGTCACAG ACGGATAGAT
    TATGTGCTCA CCTACCACAT CCAGAAGCCC CAGAACACTG GCAAACAGTC ATCCAAGTCC
    TGCCTGGGGG GTTGTTTGTG CTGCAAAAAA CGAGGCCAGA CAAACCCACC GGTTCCGGAC
    CCCGAACTAG CATCTCAGGA GCAGAGGCTG GATTACCATG ATGACGACCA GCGGCTCCGC
    AGAGAGGAGT TTGAGGAGAA CCTGCGTGAA ATGGGCCTTG AGATGGAAAA AGAGGAAGGG
    AATAAAATTC CTGGCGTGGG GTTCTTGAAG ATCCACGCTC CTTGGGAAGT TCTTTGTCGA
    GAAGCAGAGT TTATGAAACT AAAAATGCCC ACAATAAAGA AATATGAAGT AAGCCATGGA
    AACAGCATTG TGGAGAAGGC CAGCAGGTTT ATGCAGAAAT TCACAGCACC TTTGCAACCT
    AAGGTTGGGG ACCGTCAGAT TGAACACGAG AAACACCTCT CATACCCATT CTCACGAGAA
    AAGCAGCATC TCTTTGATTT ATCAGACAGG AATTCCTTCT TCGACAGCAA AACCAGGAGC
    TCAATAGTGT ATGAGATTTT AAAGCGAACA AAATGCACAC GGGCGAAGTA TATCATGGGA
    ATCATCAGTC TTCTGGGCAA CGGTATCTAC ACTTCAGCCT ACCCTTTGCA TGATGGCGAT
    ATTGATGACG TCCATGCTGA AATAAATGAC AGAAAGATCT TATATGAAGA GTGGGCGAGT
    TACAGCATAT TCTACAAGTA TCAACCCATC GGACTGATCA GGAAGTATTT TGGTGAGAAA
    GTGGGCCTGT ACTTCGCCTG GCTGGGTGTG TACACTCAGA TGCTCATCCC AGCAGCCATT
    GTGGGCGTGA TCGTCTTCCT GTACGGCTGC GCTACTGTGG ACGACAACAT TCCCAGTATG
    GAGATCTGCG ACGAGAGAAA CAACATCACC ATGTGCCCAA TGTGCGACCG GGCCTGCAGC
    TACTGGAAAC TGGTGACGGC TTGCGGCACA GCCAGAGCCA GCCACCTGTT CGACAACGCG
    GCCACCGTCT TCTTTGCCAT CTTCATGGCA CTATGGGCCG CCATGTTTAT GGAGCACTGG
    AAGAGACGGC AAGTGAGGCT CAACTACCTC TGGGACCTGA CGGGTTTTGA GGACACCGAG
    AGTCATCCGA GAGCGGAGTA TGAGTTTCAT GTCATGAAGA AGTCATTGAG GAAAGAGAAA
    TCTCCAAAGG CAGGGAATGT GAAGCTGACA TGCACCGACA GAATGCCAGC TTACATGACC
    ATCATCATCA CCATGATTTT TCTGATCTGT GTGACCTTGG CCATCGTGTT TGGTGTGGTG
    CTATACAGGG TGTCTATTAT GACAGCCCTT CATATGAGTT CCACTCCTAC ATTCCGTTCT
    AATATACGTG CCACCGTTAA AACCACCGGC GCCATCATCA ACCTCATTAT CATCATCATC
    ATGGATGAGG TCTACGGAGC AGTTGCACGT TGGCTCACTA TTATGGAGGT GCCAAAGACT
    GACAAGAGTT TTGAGGAGAG GCTGATCTTC AAGACATTCG TATTGAAGTT TGCGAATGCA
    TTTACTCCTA TTGTGTACCT GGCGTTCTTC AGAGGCAGAC TCATTGGACG TCCTGGCAAA
    TATATCTATG TTATGGGATC TTACAGAATG GAAGAGTGCG CTCATGCCGG CTGTCTGATG
    GAACTGTGCA TTCAGCTCTG CATCACTATG CTTGGCAAAC AGCTCATCCA GAATAACCTG
    TTTGAGATCG GCGTTCCGAA AATCAAGAAG CTGCTCCGAC AACGCAAGAT TGACCCAAAG
    CACCAGGAAG AGCTGAACAA AACGCTTCAT CGCCATGAGA AGGACCACTT CCTAGGACCC
    TTTGTCGGAC TCAACCCAGA GTACATGGAA ATGATCATTC AGTTCGGGAT GGTGACCTTA
    TTCGTGGCCT CTTTTCCGCT GGCTCCTCTC TTTGCTCTGC TCAACAACAT CATTGAGATC
    CGTCTTGATG CCAAAAAGTT TGTTACGGAA CTGAGAAGGC CAATCGCTGT AAGAGCAAAG
    GACATCGGAA TCTGGTACAC TCTTCTCAGA GGACTCAGCA AAGTGGCTGT CATTGTCAAT
    GCGTTTGTTA TCTCGTTCAC CTCTGACTTC ATCCCGCGGC TGGTCTATCA ATACATGTAC
    AGTCCAGACG GGACCCTCCA TGGCTTTGTC AATCACACGC TCTCGTATTT TAACGTCACT
    CACTTCCAGC CTGGCACAGA GCCCCTGGAG CCCATGCACC TGGGATACCA AGTGGACACG
    TGCAGATATA AAGATTACAG GGATCCTCCA TGGTCTGATA CACCGTACGA GTTCTCAAGA
    GAATTCTGGG CCATTCTGGC AGCCCGCTTC GCTTTCGTAA TTCTCTTTCA GAATGTGGTG
    ATGCTGATGA GTGACTTCGT GGACTGGCTC ATCCCAGACA TCCCCAAAGA CATCAGCTTG
    AAAATCCACA AGGAGAGGAA CCTCATCATT GAGCTGTTCA TGAAGCGGGA ACAGGGAAAG
    AAGTACAGGA ACACCATGAA CGACCCAAGT CCACAACCGC TCCGCTCGCG GCCCAGTTCA
    CAAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59621
    Clone ID Related Accession (Same CDS sequence) XM_017356841.1 , XM_017356841.2
    Accession Version XM_017356841.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2775bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product anoctamin-1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTGGCGAA ATGAAAAGTA TGAATCTGTC CCGTTGGATG TGAAGGGGAT TGATGAAGAC 
    AATTCTAAAG GCACAAGGGA TTCTTTATAT CCCCACACTA CTCTCCTGAA AAGTCAGCAG
    GCGGATGAGA AACCAAAGGA CCCAAAGCTT TGGCCGTACT TCAAGGATGG TCACAGACGG
    ATAGATTATG TGCTCACCTA CCACATCCAG AAGCCCCAGA ACACTGGCAA ACAGTCATCC
    AAGTCCTGCC TGGGGGGTTG TTTGTGCTGC AAAAAACGAG GCCAGACAAA CCCACCGGTT
    CCGGACCCCG AACTAGCATC TCAGGAGCAG AGGCTGGATT ACCATGATGA CGACCAGCGG
    CTCCGCAGAG AGGAGTTTGA GGAGAACCTG CGTGAAATGG GCCTTGAGAT GGAAAAAGAG
    GAAGGGAATA AAATTCCTGG CGTGGGGTTC TTGAAGATCC ACGCTCCTTG GGAAGTTCTT
    TGTCGAGAAG CAGAGTTTAT GAAACTAAAA ATGCCCACAA TAAAGAAATA TGAAGTAAGC
    CATGGAAACA GCATTGTGGA GAAGGCCAGC AGGTTTATGC AGAAATTCAC AGCACCTTTG
    CAACCTAAGG TTGGGGACCG TCAGATTGAA CACGAGAAAC ACCTCTCATA CCCATTCTCA
    CGAGAAAAGC AGCATCTCTT TGATTTATCA GACAGGAATT CCTTCTTCGA CAGCAAAACC
    AGGAGCTCAA TAGTGTATGA GATTTTAAAG CGAACAAAAT GCACACGGGC GAAGTATATC
    ATGGGAATCA TCAGTCTTCT GGGCAACGGT ATCTACACTT CAGCCTACCC TTTGCATGAT
    GGCGATATTG ATGACGTCCA TGCTGAAATA AATGACAGAA AGATCTTATA TGAAGAGTGG
    GCGAGTTACA GCATATTCTA CAAGTATCAA CCCATCGGAC TGATCAGGAA GTATTTTGGT
    GAGAAAGTGG GCCTGTACTT CGCCTGGCTG GGTGTGTACA CTCAGATGCT CATCCCAGCA
    GCCATTGTGG GCGTGATCGT CTTCCTGTAC GGCTGCGCTA CTGTGGACGA CAACATTCCC
    AGTATGGAGA TCTGCGACGA GAGAAACAAC ATCACCATGT GCCCAATGTG CGACCGGGCC
    TGCAGCTACT GGAAACTGGT GACGGCTTGC GGCACAGCCA GAGCCAGCCA CCTGTTCGAC
    AACGCGGCCA CCGTCTTCTT TGCCATCTTC ATGGCACTAT GGGCCGCCAT GTTTATGGAG
    CACTGGAAGA GACGGCAAGT GAGGCTCAAC TACCTCTGGG ACCTGACGGG TTTTGAGGAC
    ACCGAGAGTC ATCCGAGAGC GGAGTATGAG TTTCATGTCA TGAAGAAGTC ATTGAGGAAA
    GAGAAATCTC CAAAGGCAGG GAATGTGAAG CTGACATGCA CCGACAGAAT GCCAGCTTAC
    ATGACCATCA TCATCACCAT GATTTTTCTG ATCTGTGTGA CCTTGGCCAT CGTGTTTGGT
    GTGGTGCTAT ACAGGGTGTC TATTATGACA GCCCTTCATA TGAGTTCCAC TCCTACATTC
    CGTTCTAATA TACGTGCCAC CGTTAAAACC ACCGGCGCCA TCATCAACCT CATTATCATC
    ATCATCATGG ATGAGGTCTA CGGAGCAGTT GCACGTTGGC TCACTATTAT GGAGGTGCCA
    AAGACTGACA AGAGTTTTGA GGAGAGGCTG ATCTTCAAGA CATTCGTATT GAAGTTTGCG
    AATGCATTTA CTCCTATTGT GTACCTGGCG TTCTTCAGAG GCAGACTCAT TGGACGTCCT
    GGCAAATATA TCTATGTTAT GGGATCTTAC AGAATGGAAG AGTGCGCTCA TGCCGGCTGT
    CTGATGGAAC TGTGCATTCA GCTCTGCATC ACTATGCTTG GCAAACAGCT CATCCAGAAT
    AACCTGTTTG AGATCGGCGT TCCGAAAATC AAGAAGCTGC TCCGACAACG CAAGATTGAC
    CCAAAGCACC AGGAAGAGCT GAACAAAACG CTTCATCGCC ATGAGAAGGA CCACTTCCTA
    GGACCCTTTG TCGGACTCAA CCCAGAGTAC ATGGAAATGA TCATTCAGTT CGGGATGGTG
    ACCTTATTCG TGGCCTCTTT TCCGCTGGCT CCTCTCTTTG CTCTGCTCAA CAACATCATT
    GAGATCCGTC TTGATGCCAA AAAGTTTGTT ACGGAACTGA GAAGGCCAAT CGCTGTAAGA
    GCAAAGGACA TCGGAATCTG GTACACTCTT CTCAGAGGAC TCAGCAAAGT GGCTGTCATT
    GTCAATGCGT TTGTTATCTC GTTCACCTCT GACTTCATCC CGCGGCTGGT CTATCAATAC
    ATGTACAGTC CAGACGGGAC CCTCCATGGC TTTGTCAATC ACACGCTCTC GTATTTTAAC
    GTCACTCACT TCCAGCCTGG CACAGAGCCC CTGGAGCCCA TGCACCTGGG ATACCAAGTG
    GACACGTGCA GATATAAAGA TTACAGGGAT CCTCCATGGT CTGATACACC GTACGAGTTC
    TCAAGAGAAT TCTGGGCCAT TCTGGCAGCC CGCTTCGCTT TCGTAATTCT CTTTCAGAAT
    GTGGTGATGC TGATGAGTGA CTTCGTGGAC TGGCTCATCC CAGACATCCC CAAAGACATC
    AGCTTGAAAA TCCACAAGGA GAGGAACCTC ATCATTGAGC TGTTCATGAA GCGGGAACAG
    GGAAAGAAGT ACAGGAACAC CATGAACGAC CCAAGTCCAC AACCGCTCCG CTCGCGGCCC
    AGTTCACAAG CGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212330.1
    CDS299..3073
    Translation

    Target ORF information:

    RefSeq Version XM_017356841.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio anoctamin 1, calcium activated chloride channel (ano1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356841.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTGGCGAA ATGAAAAGTA TGAATCTGTC CCGTTGGATG TGAAGGGGAT TGATGAAGAC 
    AATTCTAAAG GCACAAGGGA TTCTTTATAT CCCCACACTA CTCTCCTGAA AAGTCAGCAG
    GCGGATGAGA AACCAAAGGA CCCAAAGCTT TGGCCGTACT TCAAGGATGG TCACAGACGG
    ATAGATTATG TGCTCACCTA CCACATCCAG AAGCCCCAGA ACACTGGCAA ACAGTCATCC
    AAGTCCTGCC TGGGGGGTTG TTTGTGCTGC AAAAAACGAG GCCAGACAAA CCCACCGGTT
    CCGGACCCCG AACTAGCATC TCAGGAGCAG AGGCTGGATT ACCATGATGA CGACCAGCGG
    CTCCGCAGAG AGGAGTTTGA GGAGAACCTG CGTGAAATGG GCCTTGAGAT GGAAAAAGAG
    GAAGGGAATA AAATTCCTGG CGTGGGGTTC TTGAAGATCC ACGCTCCTTG GGAAGTTCTT
    TGTCGAGAAG CAGAGTTTAT GAAACTAAAA ATGCCCACAA TAAAGAAATA TGAAGTAAGC
    CATGGAAACA GCATTGTGGA GAAGGCCAGC AGGTTTATGC AGAAATTCAC AGCACCTTTG
    CAACCTAAGG TTGGGGACCG TCAGATTGAA CACGAGAAAC ACCTCTCATA CCCATTCTCA
    CGAGAAAAGC AGCATCTCTT TGATTTATCA GACAGGAATT CCTTCTTCGA CAGCAAAACC
    AGGAGCTCAA TAGTGTATGA GATTTTAAAG CGAACAAAAT GCACACGGGC GAAGTATATC
    ATGGGAATCA TCAGTCTTCT GGGCAACGGT ATCTACACTT CAGCCTACCC TTTGCATGAT
    GGCGATATTG ATGACGTCCA TGCTGAAATA AATGACAGAA AGATCTTATA TGAAGAGTGG
    GCGAGTTACA GCATATTCTA CAAGTATCAA CCCATCGGAC TGATCAGGAA GTATTTTGGT
    GAGAAAGTGG GCCTGTACTT CGCCTGGCTG GGTGTGTACA CTCAGATGCT CATCCCAGCA
    GCCATTGTGG GCGTGATCGT CTTCCTGTAC GGCTGCGCTA CTGTGGACGA CAACATTCCC
    AGTATGGAGA TCTGCGACGA GAGAAACAAC ATCACCATGT GCCCAATGTG CGACCGGGCC
    TGCAGCTACT GGAAACTGGT GACGGCTTGC GGCACAGCCA GAGCCAGCCA CCTGTTCGAC
    AACGCGGCCA CCGTCTTCTT TGCCATCTTC ATGGCACTAT GGGCCGCCAT GTTTATGGAG
    CACTGGAAGA GACGGCAAGT GAGGCTCAAC TACCTCTGGG ACCTGACGGG TTTTGAGGAC
    ACCGAGAGTC ATCCGAGAGC GGAGTATGAG TTTCATGTCA TGAAGAAGTC ATTGAGGAAA
    GAGAAATCTC CAAAGGCAGG GAATGTGAAG CTGACATGCA CCGACAGAAT GCCAGCTTAC
    ATGACCATCA TCATCACCAT GATTTTTCTG ATCTGTGTGA CCTTGGCCAT CGTGTTTGGT
    GTGGTGCTAT ACAGGGTGTC TATTATGACA GCCCTTCATA TGAGTTCCAC TCCTACATTC
    CGTTCTAATA TACGTGCCAC CGTTAAAACC ACCGGCGCCA TCATCAACCT CATTATCATC
    ATCATCATGG ATGAGGTCTA CGGAGCAGTT GCACGTTGGC TCACTATTAT GGAGGTGCCA
    AAGACTGACA AGAGTTTTGA GGAGAGGCTG ATCTTCAAGA CATTCGTATT GAAGTTTGCG
    AATGCATTTA CTCCTATTGT GTACCTGGCG TTCTTCAGAG GCAGACTCAT TGGACGTCCT
    GGCAAATATA TCTATGTTAT GGGATCTTAC AGAATGGAAG AGTGCGCTCA TGCCGGCTGT
    CTGATGGAAC TGTGCATTCA GCTCTGCATC ACTATGCTTG GCAAACAGCT CATCCAGAAT
    AACCTGTTTG AGATCGGCGT TCCGAAAATC AAGAAGCTGC TCCGACAACG CAAGATTGAC
    CCAAAGCACC AGGAAGAGCT GAACAAAACG CTTCATCGCC ATGAGAAGGA CCACTTCCTA
    GGACCCTTTG TCGGACTCAA CCCAGAGTAC ATGGAAATGA TCATTCAGTT CGGGATGGTG
    ACCTTATTCG TGGCCTCTTT TCCGCTGGCT CCTCTCTTTG CTCTGCTCAA CAACATCATT
    GAGATCCGTC TTGATGCCAA AAAGTTTGTT ACGGAACTGA GAAGGCCAAT CGCTGTAAGA
    GCAAAGGACA TCGGAATCTG GTACACTCTT CTCAGAGGAC TCAGCAAAGT GGCTGTCATT
    GTCAATGCGT TTGTTATCTC GTTCACCTCT GACTTCATCC CGCGGCTGGT CTATCAATAC
    ATGTACAGTC CAGACGGGAC CCTCCATGGC TTTGTCAATC ACACGCTCTC GTATTTTAAC
    GTCACTCACT TCCAGCCTGG CACAGAGCCC CTGGAGCCCA TGCACCTGGG ATACCAAGTG
    GACACGTGCA GATATAAAGA TTACAGGGAT CCTCCATGGT CTGATACACC GTACGAGTTC
    TCAAGAGAAT TCTGGGCCAT TCTGGCAGCC CGCTTCGCTT TCGTAATTCT CTTTCAGAAT
    GTGGTGATGC TGATGAGTGA CTTCGTGGAC TGGCTCATCC CAGACATCCC CAAAGACATC
    AGCTTGAAAA TCCACAAGGA GAGGAACCTC ATCATTGAGC TGTTCATGAA GCGGGAACAG
    GGAAAGAAGT ACAGGAACAC CATGAACGAC CCAAGTCCAC AACCGCTCCG CTCGCGGCCC
    AGTTCACAAG CGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59621
    Clone ID Related Accession (Same CDS sequence) XM_017356841.1 , XM_017356841.2
    Accession Version XM_017356841.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2775bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product anoctamin-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356841.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTGGCGAA ATGAAAAGTA TGAATCTGTC CCGTTGGATG TGAAGGGGAT TGATGAAGAC 
    AATTCTAAAG GCACAAGGGA TTCTTTATAT CCCCACACTA CTCTCCTGAA AAGTCAGCAG
    GCGGATGAGA AACCAAAGGA CCCAAAGCTT TGGCCGTACT TCAAGGATGG TCACAGACGG
    ATAGATTATG TGCTCACCTA CCACATCCAG AAGCCCCAGA ACACTGGCAA ACAGTCATCC
    AAGTCCTGCC TGGGGGGTTG TTTGTGCTGC AAAAAACGAG GCCAGACAAA CCCACCGGTT
    CCGGACCCCG AACTAGCATC TCAGGAGCAG AGGCTGGATT ACCATGATGA CGACCAGCGG
    CTCCGCAGAG AGGAGTTTGA GGAGAACCTG CGTGAAATGG GCCTTGAGAT GGAAAAAGAG
    GAAGGGAATA AAATTCCTGG CGTGGGGTTC TTGAAGATCC ACGCTCCTTG GGAAGTTCTT
    TGTCGAGAAG CAGAGTTTAT GAAACTAAAA ATGCCCACAA TAAAGAAATA TGAAGTAAGC
    CATGGAAACA GCATTGTGGA GAAGGCCAGC AGGTTTATGC AGAAATTCAC AGCACCTTTG
    CAACCTAAGG TTGGGGACCG TCAGATTGAA CACGAGAAAC ACCTCTCATA CCCATTCTCA
    CGAGAAAAGC AGCATCTCTT TGATTTATCA GACAGGAATT CCTTCTTCGA CAGCAAAACC
    AGGAGCTCAA TAGTGTATGA GATTTTAAAG CGAACAAAAT GCACACGGGC GAAGTATATC
    ATGGGAATCA TCAGTCTTCT GGGCAACGGT ATCTACACTT CAGCCTACCC TTTGCATGAT
    GGCGATATTG ATGACGTCCA TGCTGAAATA AATGACAGAA AGATCTTATA TGAAGAGTGG
    GCGAGTTACA GCATATTCTA CAAGTATCAA CCCATCGGAC TGATCAGGAA GTATTTTGGT
    GAGAAAGTGG GCCTGTACTT CGCCTGGCTG GGTGTGTACA CTCAGATGCT CATCCCAGCA
    GCCATTGTGG GCGTGATCGT CTTCCTGTAC GGCTGCGCTA CTGTGGACGA CAACATTCCC
    AGTATGGAGA TCTGCGACGA GAGAAACAAC ATCACCATGT GCCCAATGTG CGACCGGGCC
    TGCAGCTACT GGAAACTGGT GACGGCTTGC GGCACAGCCA GAGCCAGCCA CCTGTTCGAC
    AACGCGGCCA CCGTCTTCTT TGCCATCTTC ATGGCACTAT GGGCCGCCAT GTTTATGGAG
    CACTGGAAGA GACGGCAAGT GAGGCTCAAC TACCTCTGGG ACCTGACGGG TTTTGAGGAC
    ACCGAGAGTC ATCCGAGAGC GGAGTATGAG TTTCATGTCA TGAAGAAGTC ATTGAGGAAA
    GAGAAATCTC CAAAGGCAGG GAATGTGAAG CTGACATGCA CCGACAGAAT GCCAGCTTAC
    ATGACCATCA TCATCACCAT GATTTTTCTG ATCTGTGTGA CCTTGGCCAT CGTGTTTGGT
    GTGGTGCTAT ACAGGGTGTC TATTATGACA GCCCTTCATA TGAGTTCCAC TCCTACATTC
    CGTTCTAATA TACGTGCCAC CGTTAAAACC ACCGGCGCCA TCATCAACCT CATTATCATC
    ATCATCATGG ATGAGGTCTA CGGAGCAGTT GCACGTTGGC TCACTATTAT GGAGGTGCCA
    AAGACTGACA AGAGTTTTGA GGAGAGGCTG ATCTTCAAGA CATTCGTATT GAAGTTTGCG
    AATGCATTTA CTCCTATTGT GTACCTGGCG TTCTTCAGAG GCAGACTCAT TGGACGTCCT
    GGCAAATATA TCTATGTTAT GGGATCTTAC AGAATGGAAG AGTGCGCTCA TGCCGGCTGT
    CTGATGGAAC TGTGCATTCA GCTCTGCATC ACTATGCTTG GCAAACAGCT CATCCAGAAT
    AACCTGTTTG AGATCGGCGT TCCGAAAATC AAGAAGCTGC TCCGACAACG CAAGATTGAC
    CCAAAGCACC AGGAAGAGCT GAACAAAACG CTTCATCGCC ATGAGAAGGA CCACTTCCTA
    GGACCCTTTG TCGGACTCAA CCCAGAGTAC ATGGAAATGA TCATTCAGTT CGGGATGGTG
    ACCTTATTCG TGGCCTCTTT TCCGCTGGCT CCTCTCTTTG CTCTGCTCAA CAACATCATT
    GAGATCCGTC TTGATGCCAA AAAGTTTGTT ACGGAACTGA GAAGGCCAAT CGCTGTAAGA
    GCAAAGGACA TCGGAATCTG GTACACTCTT CTCAGAGGAC TCAGCAAAGT GGCTGTCATT
    GTCAATGCGT TTGTTATCTC GTTCACCTCT GACTTCATCC CGCGGCTGGT CTATCAATAC
    ATGTACAGTC CAGACGGGAC CCTCCATGGC TTTGTCAATC ACACGCTCTC GTATTTTAAC
    GTCACTCACT TCCAGCCTGG CACAGAGCCC CTGGAGCCCA TGCACCTGGG ATACCAAGTG
    GACACGTGCA GATATAAAGA TTACAGGGAT CCTCCATGGT CTGATACACC GTACGAGTTC
    TCAAGAGAAT TCTGGGCCAT TCTGGCAGCC CGCTTCGCTT TCGTAATTCT CTTTCAGAAT
    GTGGTGATGC TGATGAGTGA CTTCGTGGAC TGGCTCATCC CAGACATCCC CAAAGACATC
    AGCTTGAAAA TCCACAAGGA GAGGAACCTC ATCATTGAGC TGTTCATGAA GCGGGAACAG
    GGAAAGAAGT ACAGGAACAC CATGAACGAC CCAAGTCCAC AACCGCTCCG CTCGCGGCCC
    AGTTCACAAG CGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212330.1
    CDS298..3072
    Translation

    Target ORF information:

    RefSeq Version XM_017356841.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio anoctamin 1, calcium activated chloride channel (ano1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356841.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTGGCGAA ATGAAAAGTA TGAATCTGTC CCGTTGGATG TGAAGGGGAT TGATGAAGAC 
    AATTCTAAAG GCACAAGGGA TTCTTTATAT CCCCACACTA CTCTCCTGAA AAGTCAGCAG
    GCGGATGAGA AACCAAAGGA CCCAAAGCTT TGGCCGTACT TCAAGGATGG TCACAGACGG
    ATAGATTATG TGCTCACCTA CCACATCCAG AAGCCCCAGA ACACTGGCAA ACAGTCATCC
    AAGTCCTGCC TGGGGGGTTG TTTGTGCTGC AAAAAACGAG GCCAGACAAA CCCACCGGTT
    CCGGACCCCG AACTAGCATC TCAGGAGCAG AGGCTGGATT ACCATGATGA CGACCAGCGG
    CTCCGCAGAG AGGAGTTTGA GGAGAACCTG CGTGAAATGG GCCTTGAGAT GGAAAAAGAG
    GAAGGGAATA AAATTCCTGG CGTGGGGTTC TTGAAGATCC ACGCTCCTTG GGAAGTTCTT
    TGTCGAGAAG CAGAGTTTAT GAAACTAAAA ATGCCCACAA TAAAGAAATA TGAAGTAAGC
    CATGGAAACA GCATTGTGGA GAAGGCCAGC AGGTTTATGC AGAAATTCAC AGCACCTTTG
    CAACCTAAGG TTGGGGACCG TCAGATTGAA CACGAGAAAC ACCTCTCATA CCCATTCTCA
    CGAGAAAAGC AGCATCTCTT TGATTTATCA GACAGGAATT CCTTCTTCGA CAGCAAAACC
    AGGAGCTCAA TAGTGTATGA GATTTTAAAG CGAACAAAAT GCACACGGGC GAAGTATATC
    ATGGGAATCA TCAGTCTTCT GGGCAACGGT ATCTACACTT CAGCCTACCC TTTGCATGAT
    GGCGATATTG ATGACGTCCA TGCTGAAATA AATGACAGAA AGATCTTATA TGAAGAGTGG
    GCGAGTTACA GCATATTCTA CAAGTATCAA CCCATCGGAC TGATCAGGAA GTATTTTGGT
    GAGAAAGTGG GCCTGTACTT CGCCTGGCTG GGTGTGTACA CTCAGATGCT CATCCCAGCA
    GCCATTGTGG GCGTGATCGT CTTCCTGTAC GGCTGCGCTA CTGTGGACGA CAACATTCCC
    AGTATGGAGA TCTGCGACGA GAGAAACAAC ATCACCATGT GCCCAATGTG CGACCGGGCC
    TGCAGCTACT GGAAACTGGT GACGGCTTGC GGCACAGCCA GAGCCAGCCA CCTGTTCGAC
    AACGCGGCCA CCGTCTTCTT TGCCATCTTC ATGGCACTAT GGGCCGCCAT GTTTATGGAG
    CACTGGAAGA GACGGCAAGT GAGGCTCAAC TACCTCTGGG ACCTGACGGG TTTTGAGGAC
    ACCGAGAGTC ATCCGAGAGC GGAGTATGAG TTTCATGTCA TGAAGAAGTC ATTGAGGAAA
    GAGAAATCTC CAAAGGCAGG GAATGTGAAG CTGACATGCA CCGACAGAAT GCCAGCTTAC
    ATGACCATCA TCATCACCAT GATTTTTCTG ATCTGTGTGA CCTTGGCCAT CGTGTTTGGT
    GTGGTGCTAT ACAGGGTGTC TATTATGACA GCCCTTCATA TGAGTTCCAC TCCTACATTC
    CGTTCTAATA TACGTGCCAC CGTTAAAACC ACCGGCGCCA TCATCAACCT CATTATCATC
    ATCATCATGG ATGAGGTCTA CGGAGCAGTT GCACGTTGGC TCACTATTAT GGAGGTGCCA
    AAGACTGACA AGAGTTTTGA GGAGAGGCTG ATCTTCAAGA CATTCGTATT GAAGTTTGCG
    AATGCATTTA CTCCTATTGT GTACCTGGCG TTCTTCAGAG GCAGACTCAT TGGACGTCCT
    GGCAAATATA TCTATGTTAT GGGATCTTAC AGAATGGAAG AGTGCGCTCA TGCCGGCTGT
    CTGATGGAAC TGTGCATTCA GCTCTGCATC ACTATGCTTG GCAAACAGCT CATCCAGAAT
    AACCTGTTTG AGATCGGCGT TCCGAAAATC AAGAAGCTGC TCCGACAACG CAAGATTGAC
    CCAAAGCACC AGGAAGAGCT GAACAAAACG CTTCATCGCC ATGAGAAGGA CCACTTCCTA
    GGACCCTTTG TCGGACTCAA CCCAGAGTAC ATGGAAATGA TCATTCAGTT CGGGATGGTG
    ACCTTATTCG TGGCCTCTTT TCCGCTGGCT CCTCTCTTTG CTCTGCTCAA CAACATCATT
    GAGATCCGTC TTGATGCCAA AAAGTTTGTT ACGGAACTGA GAAGGCCAAT CGCTGTAAGA
    GCAAAGGACA TCGGAATCTG GTACACTCTT CTCAGAGGAC TCAGCAAAGT GGCTGTCATT
    GTCAATGCGT TTGTTATCTC GTTCACCTCT GACTTCATCC CGCGGCTGGT CTATCAATAC
    ATGTACAGTC CAGACGGGAC CCTCCATGGC TTTGTCAATC ACACGCTCTC GTATTTTAAC
    GTCACTCACT TCCAGCCTGG CACAGAGCCC CTGGAGCCCA TGCACCTGGG ATACCAAGTG
    GACACGTGCA GATATAAAGA TTACAGGGAT CCTCCATGGT CTGATACACC GTACGAGTTC
    TCAAGAGAAT TCTGGGCCAT TCTGGCAGCC CGCTTCGCTT TCGTAATTCT CTTTCAGAAT
    GTGGTGATGC TGATGAGTGA CTTCGTGGAC TGGCTCATCC CAGACATCCC CAAAGACATC
    AGCTTGAAAA TCCACAAGGA GAGGAACCTC ATCATTGAGC TGTTCATGAA GCGGGAACAG
    GGAAAGAAGT ACAGGAACAC CATGAACGAC CCAAGTCCAC AACCGCTCCG CTCGCGGCCC
    AGTTCACAAG CGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.