ANO1 cDNA ORF clone, Homo sapiens(human)

The following ANO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu26085 NM_018043.6
Latest version!
Homo sapiens anoctamin 1 (ANO1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$469.50-$657.30
$939.00
OHu68992 XM_011545131.2
Latest version!
Homo sapiens anoctamin 1 (ANO1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu45395 XM_006718602.2
Latest version!
Homo sapiens anoctamin 1 (ANO1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu45397 XM_006718604.2
Latest version!
Homo sapiens anoctamin 1 (ANO1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu45398 XM_006718605.2
Latest version!
Homo sapiens anoctamin 1 (ANO1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu68987 XM_011545125.2
Latest version!
Homo sapiens anoctamin 1 (ANO1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu68988 XM_011545126.2
Latest version!
Homo sapiens anoctamin 1 (ANO1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu68989 XM_011545127.2
Latest version!
Homo sapiens anoctamin 1 (ANO1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu68990 XM_011545128.2
Latest version!
Homo sapiens anoctamin 1 (ANO1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu68991 XM_011545129.2
Latest version!
Homo sapiens anoctamin 1 (ANO1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu92337 XM_017017956.1
Latest version!
Homo sapiens anoctamin 1 (ANO1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu92338 XM_017017957.1
Latest version!
Homo sapiens anoctamin 1 (ANO1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu68983 XM_011545121.2
Latest version!
Homo sapiens anoctamin 1 (ANO1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu68985 XM_011545123.2
Latest version!
Homo sapiens anoctamin 1 (ANO1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu68986 XM_011545124.2
Latest version!
Homo sapiens anoctamin 1 (ANO1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
View Old Accession Versions >>
OHu26085 NM_018043.5 Homo sapiens anoctamin 1 (ANO1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$469.50-$657.30
$939.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu26085
    Clone ID Related Accession (Same CDS sequence) NM_018043.5 , NM_018043.6
    Accession Version NM_018043.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-17
    Organism Homo sapiens(human)
    Product anoctamin-1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY728143.1, AP000879.4, AK001123.1 and AI694083.1. On Jul 18, 2008 this sequence version replaced NM_018043.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY728143.1, AL833582.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTATCTGTGG ACCCTGATGC CGAGTGCAAG TATGGCCTGT ACTTCAGGGA CGGCCGGCGC
    AAGGTGGACT ACATCCTGGT GTACCATCAC AAGAGGCCCT CGGGCAACCG GACCCTGGTC
    AGGAGGGTGC AGCACAGCGA CACCCCCTCT GGGGCTCGCA GCGTCAAGCA GGACCACCCC
    CTGCCGGGCA AGGGGGCGTC GCTGGATGCA GGCTCGGGGG AGCCCCCGAT GGACTACCAC
    GAGGATGACA AGCGCTTCCG CAGGGAGGAG TACGAGGGCA ACCTCCTGGA GGCGGGCCTG
    GAGCTGGAGC GGGACGAGGA CACTAAAATC CACGGAGTCG GGTTTGTGAA AATCCATGCC
    CCCTGGAACG TGCTGTGCAG AGAGGCCGAG TTTCTGAAAC TGAAGATGCC GACGAAGAAG
    ATGTACCACA TTAATGAGAC CCGTGGCCTC CTGAAAAAAA TCAACTCTGT GCTCCAGAAA
    ATCACAGATC CCATCCAGCC CAAAGTGGCT GAGCACAGGC CCCAGACCAT GAAGAGACTC
    TCCTATCCCT TCTCCCGGGA GAAGCAGCAT CTATTTGACT TGTCTGATAA GGATTCCTTT
    TTCGACAGCA AAACCCGGAG CACGATTGTC TATGAGATCT TGAAGAGAAC GACGTGTACA
    AAGGCCAAGT ACAGCATGGG CATCACGAGC CTGCTGGCCA ATGGTGTGTA CGCGGCTGCA
    TACCCACTGC ACGATGGAGA CTACAACGGT GAAAACGTCG AGTTCAACGA CAGAAAACTC
    CTGTACGAAG AGTGGGCACG CTATGGAGTT TTCTATAAGT ACCAGCCCAT CGACCTGGTC
    AGGAAGTATT TTGGGGAGAA GATCGGCCTG TACTTCGCCT GGCTGGGCGT GTACACCCAG
    ATGCTCATCC CTGCCTCCAT CGTGGGAATC ATTGTCTTCC TGTACGGATG CGCCACCATG
    GATGAAAACA TCCCCAGCAT GGAGATGTGT GACCAGAGAC ACAATATCAC CATGTGCCCG
    CTTTGCGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGCGCCAC GGCCCGCGCC
    AGCCACCTCT TCGACAACCC CGCCACGGTC TTCTTCTCTG TCTTCATGGC CCTCTGGGCT
    GCCACCTTCA TGGAGCACTG GAAGCGGAAA CAGATGCGAC TCAACTACCG CTGGGACCTC
    ACGGGCTTTG AAGAGGAAGA GGAGGCTGTC AAGGATCATC CTAGAGCTGA ATACGAAGCC
    AGAGTCTTGG AGAAGTCTCT GAAGAAAGAG TCCAGAAACA AAGAGAAGCG CCGGCATATT
    CCAGAGGAGT CAACAAACAA ATGGAAGCAG AGGGTTAAGA CAGCCATGGC GGGGGTGAAA
    TTGACTGACA AAGTGAAGCT GACATGGAGA GATCGGTTCC CAGCCTACCT CACTAACTTG
    GTCTCCATCA TCTTCATGAT TGCAGTGACG TTTGCCATCG TCCTCGGCGT CATCATCTAC
    AGAATCTCCA TGGCCGCCGC CTTGGCCATG AACTCCTCCC CCTCCGTGCG GTCCAACATC
    CGGGTCACAG TCACAGCCAC CGCAGTCATC ATCAACCTAG TGGTCATCAT CCTCCTGGAC
    GAGGTGTATG GCTGCATAGC CCGATGGCTC ACCAAGATCG AGGTCCCAAA GACGGAGAAA
    AGCTTTGAGG AGAGGCTGAT CTTCAAGGCT TTCCTGCTGA AGTTTGTGAA TTCCTACACC
    CCCATCTTTT ACGTGGCGTT CTTCAAAGGC CGGTTTGTTG GACGCCCGGG CGACTACGTG
    TACATTTTCC GTTCCTTCCG AATGGAAGAG TGTGCGCCAG GGGGCTGCCT GATGGAGCTA
    TGCATCCAGC TCAGCATCAT CATGCTGGGG AAACAGCTGA TCCAGAACAA CCTGTTCGAG
    ATCGGCATCC CGAAGATGAA GAAGCTCATC CGCTACCTGA AGCTGAAGCA GCAGAGCCCC
    CCTGACCACG AGGAGTGTGT GAAGAGGAAA CAGCGGTACG AGGTGGATTA CAACCTGGAG
    CCCTTCGCGG GCCTCACCCC AGAGTACATG GAAATGATCA TCCAGTTTGG CTTCGTCACC
    CTGTTTGTCG CCTCCTTCCC CCTGGCCCCA CTGTTTGCGC TGCTGAACAA CATCATCGAG
    ATCCGCCTGG ACGCCAAAAA GTTTGTCACT GAGCTCCGAA GGCCGGTAGC TGTCAGAGCC
    AAAGACATCG GAATCTGGTA CAATATCCTC AGAGGCATTG GGAAGCTTGC TGTCATCATC
    AATGCCTTCG TGATCTCCTT CACGTCTGAC TTCATCCCGC GCCTGGTGTA CCTCTACATG
    TACAGTAAGA ACGGGACCAT GCACGGCTTC GTCAACCACA CCCTCTCCTC CTTCAACGTC
    AGTGACTTCC AGAACGGCAC GGCCCCCAAT GACCCCCTGG ACCTGGGCTA CGAGGTGCAG
    ATCTGCAGGT ATAAAGACTA CCGAGAGCCG CCGTGGTCGG AAAACAAGTA CGACATCTCC
    AAGGACTTCT GGGCCGTCCT GGCAGCCCGG CTGGCGTTTG TCATCGTCTT CCAGAACCTG
    GTCATGTTCA TGAGCGACTT TGTGGACTGG GTCATCCCGG ACATCCCCAA GGACATCAGC
    CAGCAGATCC ACAAGGAGAA GGTGCTCATG GTGGAGCTGT TCATGCGGGA GGAGCAAGAC
    AAGCAGCAGC TGCTGGAAAC CTGGATGGAG AAGGAGCGGC AGAAGGACGA GCCGCCGTGC
    AACCACCACA ACACCAAAGC CTGCCCAGAC AGCCTCGGCA GCCCAGCCCC CAGCCATGCC
    TACCACGGGG GCGTCCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060513.5
    CDS306..3266
    Translation

    Target ORF information:

    RefSeq Version NM_018043.5
    Organism Homo sapiens(human)
    Definition Homo sapiens anoctamin 1 (ANO1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_018043.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTATCTGTGG ACCCTGATGC CGAGTGCAAG TATGGCCTGT ACTTCAGGGA CGGCCGGCGC
    AAGGTGGACT ACATCCTGGT GTACCATCAC AAGAGGCCCT CGGGCAACCG GACCCTGGTC
    AGGAGGGTGC AGCACAGCGA CACCCCCTCT GGGGCTCGCA GCGTCAAGCA GGACCACCCC
    CTGCCGGGCA AGGGGGCGTC GCTGGATGCA GGCTCGGGGG AGCCCCCGAT GGACTACCAC
    GAGGATGACA AGCGCTTCCG CAGGGAGGAG TACGAGGGCA ACCTCCTGGA GGCGGGCCTG
    GAGCTGGAGC GGGACGAGGA CACTAAAATC CACGGAGTCG GGTTTGTGAA AATCCATGCC
    CCCTGGAACG TGCTGTGCAG AGAGGCCGAG TTTCTGAAAC TGAAGATGCC GACGAAGAAG
    ATGTACCACA TTAATGAGAC CCGTGGCCTC CTGAAAAAAA TCAACTCTGT GCTCCAGAAA
    ATCACAGATC CCATCCAGCC CAAAGTGGCT GAGCACAGGC CCCAGACCAT GAAGAGACTC
    TCCTATCCCT TCTCCCGGGA GAAGCAGCAT CTATTTGACT TGTCTGATAA GGATTCCTTT
    TTCGACAGCA AAACCCGGAG CACGATTGTC TATGAGATCT TGAAGAGAAC GACGTGTACA
    AAGGCCAAGT ACAGCATGGG CATCACGAGC CTGCTGGCCA ATGGTGTGTA CGCGGCTGCA
    TACCCACTGC ACGATGGAGA CTACAACGGT GAAAACGTCG AGTTCAACGA CAGAAAACTC
    CTGTACGAAG AGTGGGCACG CTATGGAGTT TTCTATAAGT ACCAGCCCAT CGACCTGGTC
    AGGAAGTATT TTGGGGAGAA GATCGGCCTG TACTTCGCCT GGCTGGGCGT GTACACCCAG
    ATGCTCATCC CTGCCTCCAT CGTGGGAATC ATTGTCTTCC TGTACGGATG CGCCACCATG
    GATGAAAACA TCCCCAGCAT GGAGATGTGT GACCAGAGAC ACAATATCAC CATGTGCCCG
    CTTTGCGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGCGCCAC GGCCCGCGCC
    AGCCACCTCT TCGACAACCC CGCCACGGTC TTCTTCTCTG TCTTCATGGC CCTCTGGGCT
    GCCACCTTCA TGGAGCACTG GAAGCGGAAA CAGATGCGAC TCAACTACCG CTGGGACCTC
    ACGGGCTTTG AAGAGGAAGA GGAGGCTGTC AAGGATCATC CTAGAGCTGA ATACGAAGCC
    AGAGTCTTGG AGAAGTCTCT GAAGAAAGAG TCCAGAAACA AAGAGAAGCG CCGGCATATT
    CCAGAGGAGT CAACAAACAA ATGGAAGCAG AGGGTTAAGA CAGCCATGGC GGGGGTGAAA
    TTGACTGACA AAGTGAAGCT GACATGGAGA GATCGGTTCC CAGCCTACCT CACTAACTTG
    GTCTCCATCA TCTTCATGAT TGCAGTGACG TTTGCCATCG TCCTCGGCGT CATCATCTAC
    AGAATCTCCA TGGCCGCCGC CTTGGCCATG AACTCCTCCC CCTCCGTGCG GTCCAACATC
    CGGGTCACAG TCACAGCCAC CGCAGTCATC ATCAACCTAG TGGTCATCAT CCTCCTGGAC
    GAGGTGTATG GCTGCATAGC CCGATGGCTC ACCAAGATCG AGGTCCCAAA GACGGAGAAA
    AGCTTTGAGG AGAGGCTGAT CTTCAAGGCT TTCCTGCTGA AGTTTGTGAA TTCCTACACC
    CCCATCTTTT ACGTGGCGTT CTTCAAAGGC CGGTTTGTTG GACGCCCGGG CGACTACGTG
    TACATTTTCC GTTCCTTCCG AATGGAAGAG TGTGCGCCAG GGGGCTGCCT GATGGAGCTA
    TGCATCCAGC TCAGCATCAT CATGCTGGGG AAACAGCTGA TCCAGAACAA CCTGTTCGAG
    ATCGGCATCC CGAAGATGAA GAAGCTCATC CGCTACCTGA AGCTGAAGCA GCAGAGCCCC
    CCTGACCACG AGGAGTGTGT GAAGAGGAAA CAGCGGTACG AGGTGGATTA CAACCTGGAG
    CCCTTCGCGG GCCTCACCCC AGAGTACATG GAAATGATCA TCCAGTTTGG CTTCGTCACC
    CTGTTTGTCG CCTCCTTCCC CCTGGCCCCA CTGTTTGCGC TGCTGAACAA CATCATCGAG
    ATCCGCCTGG ACGCCAAAAA GTTTGTCACT GAGCTCCGAA GGCCGGTAGC TGTCAGAGCC
    AAAGACATCG GAATCTGGTA CAATATCCTC AGAGGCATTG GGAAGCTTGC TGTCATCATC
    AATGCCTTCG TGATCTCCTT CACGTCTGAC TTCATCCCGC GCCTGGTGTA CCTCTACATG
    TACAGTAAGA ACGGGACCAT GCACGGCTTC GTCAACCACA CCCTCTCCTC CTTCAACGTC
    AGTGACTTCC AGAACGGCAC GGCCCCCAAT GACCCCCTGG ACCTGGGCTA CGAGGTGCAG
    ATCTGCAGGT ATAAAGACTA CCGAGAGCCG CCGTGGTCGG AAAACAAGTA CGACATCTCC
    AAGGACTTCT GGGCCGTCCT GGCAGCCCGG CTGGCGTTTG TCATCGTCTT CCAGAACCTG
    GTCATGTTCA TGAGCGACTT TGTGGACTGG GTCATCCCGG ACATCCCCAA GGACATCAGC
    CAGCAGATCC ACAAGGAGAA GGTGCTCATG GTGGAGCTGT TCATGCGGGA GGAGCAAGAC
    AAGCAGCAGC TGCTGGAAAC CTGGATGGAG AAGGAGCGGC AGAAGGACGA GCCGCCGTGC
    AACCACCACA ACACCAAAGC CTGCCCAGAC AGCCTCGGCA GCCCAGCCCC CAGCCATGCC
    TACCACGGGG GCGTCCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26085
    Clone ID Related Accession (Same CDS sequence) NM_018043.5 , NM_018043.6
    Accession Version NM_018043.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-10
    Organism Homo sapiens(human)
    Product anoctamin-1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP000879.4. On Jun 2, 2019 this sequence version replaced NM_018043.5. Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY728143.1, AL833582.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTATCTGTGG ACCCTGATGC CGAGTGCAAG TATGGCCTGT ACTTCAGGGA CGGCCGGCGC
    AAGGTGGACT ACATCCTGGT GTACCATCAC AAGAGGCCCT CGGGCAACCG GACCCTGGTC
    AGGAGGGTGC AGCACAGCGA CACCCCCTCT GGGGCTCGCA GCGTCAAGCA GGACCACCCC
    CTGCCGGGCA AGGGGGCGTC GCTGGATGCA GGCTCGGGGG AGCCCCCGAT GGACTACCAC
    GAGGATGACA AGCGCTTCCG CAGGGAGGAG TACGAGGGCA ACCTCCTGGA GGCGGGCCTG
    GAGCTGGAGC GGGACGAGGA CACTAAAATC CACGGAGTCG GGTTTGTGAA AATCCATGCC
    CCCTGGAACG TGCTGTGCAG AGAGGCCGAG TTTCTGAAAC TGAAGATGCC GACGAAGAAG
    ATGTACCACA TTAATGAGAC CCGTGGCCTC CTGAAAAAAA TCAACTCTGT GCTCCAGAAA
    ATCACAGATC CCATCCAGCC CAAAGTGGCT GAGCACAGGC CCCAGACCAT GAAGAGACTC
    TCCTATCCCT TCTCCCGGGA GAAGCAGCAT CTATTTGACT TGTCTGATAA GGATTCCTTT
    TTCGACAGCA AAACCCGGAG CACGATTGTC TATGAGATCT TGAAGAGAAC GACGTGTACA
    AAGGCCAAGT ACAGCATGGG CATCACGAGC CTGCTGGCCA ATGGTGTGTA CGCGGCTGCA
    TACCCACTGC ACGATGGAGA CTACAACGGT GAAAACGTCG AGTTCAACGA CAGAAAACTC
    CTGTACGAAG AGTGGGCACG CTATGGAGTT TTCTATAAGT ACCAGCCCAT CGACCTGGTC
    AGGAAGTATT TTGGGGAGAA GATCGGCCTG TACTTCGCCT GGCTGGGCGT GTACACCCAG
    ATGCTCATCC CTGCCTCCAT CGTGGGAATC ATTGTCTTCC TGTACGGATG CGCCACCATG
    GATGAAAACA TCCCCAGCAT GGAGATGTGT GACCAGAGAC ACAATATCAC CATGTGCCCG
    CTTTGCGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGCGCCAC GGCCCGCGCC
    AGCCACCTCT TCGACAACCC CGCCACGGTC TTCTTCTCTG TCTTCATGGC CCTCTGGGCT
    GCCACCTTCA TGGAGCACTG GAAGCGGAAA CAGATGCGAC TCAACTACCG CTGGGACCTC
    ACGGGCTTTG AAGAGGAAGA GGAGGCTGTC AAGGATCATC CTAGAGCTGA ATACGAAGCC
    AGAGTCTTGG AGAAGTCTCT GAAGAAAGAG TCCAGAAACA AAGAGAAGCG CCGGCATATT
    CCAGAGGAGT CAACAAACAA ATGGAAGCAG AGGGTTAAGA CAGCCATGGC GGGGGTGAAA
    TTGACTGACA AAGTGAAGCT GACATGGAGA GATCGGTTCC CAGCCTACCT CACTAACTTG
    GTCTCCATCA TCTTCATGAT TGCAGTGACG TTTGCCATCG TCCTCGGCGT CATCATCTAC
    AGAATCTCCA TGGCCGCCGC CTTGGCCATG AACTCCTCCC CCTCCGTGCG GTCCAACATC
    CGGGTCACAG TCACAGCCAC CGCAGTCATC ATCAACCTAG TGGTCATCAT CCTCCTGGAC
    GAGGTGTATG GCTGCATAGC CCGATGGCTC ACCAAGATCG AGGTCCCAAA GACGGAGAAA
    AGCTTTGAGG AGAGGCTGAT CTTCAAGGCT TTCCTGCTGA AGTTTGTGAA TTCCTACACC
    CCCATCTTTT ACGTGGCGTT CTTCAAAGGC CGGTTTGTTG GACGCCCGGG CGACTACGTG
    TACATTTTCC GTTCCTTCCG AATGGAAGAG TGTGCGCCAG GGGGCTGCCT GATGGAGCTA
    TGCATCCAGC TCAGCATCAT CATGCTGGGG AAACAGCTGA TCCAGAACAA CCTGTTCGAG
    ATCGGCATCC CGAAGATGAA GAAGCTCATC CGCTACCTGA AGCTGAAGCA GCAGAGCCCC
    CCTGACCACG AGGAGTGTGT GAAGAGGAAA CAGCGGTACG AGGTGGATTA CAACCTGGAG
    CCCTTCGCGG GCCTCACCCC AGAGTACATG GAAATGATCA TCCAGTTTGG CTTCGTCACC
    CTGTTTGTCG CCTCCTTCCC CCTGGCCCCA CTGTTTGCGC TGCTGAACAA CATCATCGAG
    ATCCGCCTGG ACGCCAAAAA GTTTGTCACT GAGCTCCGAA GGCCGGTAGC TGTCAGAGCC
    AAAGACATCG GAATCTGGTA CAATATCCTC AGAGGCATTG GGAAGCTTGC TGTCATCATC
    AATGCCTTCG TGATCTCCTT CACGTCTGAC TTCATCCCGC GCCTGGTGTA CCTCTACATG
    TACAGTAAGA ACGGGACCAT GCACGGCTTC GTCAACCACA CCCTCTCCTC CTTCAACGTC
    AGTGACTTCC AGAACGGCAC GGCCCCCAAT GACCCCCTGG ACCTGGGCTA CGAGGTGCAG
    ATCTGCAGGT ATAAAGACTA CCGAGAGCCG CCGTGGTCGG AAAACAAGTA CGACATCTCC
    AAGGACTTCT GGGCCGTCCT GGCAGCCCGG CTGGCGTTTG TCATCGTCTT CCAGAACCTG
    GTCATGTTCA TGAGCGACTT TGTGGACTGG GTCATCCCGG ACATCCCCAA GGACATCAGC
    CAGCAGATCC ACAAGGAGAA GGTGCTCATG GTGGAGCTGT TCATGCGGGA GGAGCAAGAC
    AAGCAGCAGC TGCTGGAAAC CTGGATGGAG AAGGAGCGGC AGAAGGACGA GCCGCCGTGC
    AACCACCACA ACACCAAAGC CTGCCCAGAC AGCCTCGGCA GCCCAGCCCC CAGCCATGCC
    TACCACGGGG GCGTCCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060513.5
    CDS68..3028
    Translation

    Target ORF information:

    RefSeq Version NM_018043.6
    Organism Homo sapiens(human)
    Definition Homo sapiens anoctamin 1 (ANO1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_018043.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTATCTGTGG ACCCTGATGC CGAGTGCAAG TATGGCCTGT ACTTCAGGGA CGGCCGGCGC
    AAGGTGGACT ACATCCTGGT GTACCATCAC AAGAGGCCCT CGGGCAACCG GACCCTGGTC
    AGGAGGGTGC AGCACAGCGA CACCCCCTCT GGGGCTCGCA GCGTCAAGCA GGACCACCCC
    CTGCCGGGCA AGGGGGCGTC GCTGGATGCA GGCTCGGGGG AGCCCCCGAT GGACTACCAC
    GAGGATGACA AGCGCTTCCG CAGGGAGGAG TACGAGGGCA ACCTCCTGGA GGCGGGCCTG
    GAGCTGGAGC GGGACGAGGA CACTAAAATC CACGGAGTCG GGTTTGTGAA AATCCATGCC
    CCCTGGAACG TGCTGTGCAG AGAGGCCGAG TTTCTGAAAC TGAAGATGCC GACGAAGAAG
    ATGTACCACA TTAATGAGAC CCGTGGCCTC CTGAAAAAAA TCAACTCTGT GCTCCAGAAA
    ATCACAGATC CCATCCAGCC CAAAGTGGCT GAGCACAGGC CCCAGACCAT GAAGAGACTC
    TCCTATCCCT TCTCCCGGGA GAAGCAGCAT CTATTTGACT TGTCTGATAA GGATTCCTTT
    TTCGACAGCA AAACCCGGAG CACGATTGTC TATGAGATCT TGAAGAGAAC GACGTGTACA
    AAGGCCAAGT ACAGCATGGG CATCACGAGC CTGCTGGCCA ATGGTGTGTA CGCGGCTGCA
    TACCCACTGC ACGATGGAGA CTACAACGGT GAAAACGTCG AGTTCAACGA CAGAAAACTC
    CTGTACGAAG AGTGGGCACG CTATGGAGTT TTCTATAAGT ACCAGCCCAT CGACCTGGTC
    AGGAAGTATT TTGGGGAGAA GATCGGCCTG TACTTCGCCT GGCTGGGCGT GTACACCCAG
    ATGCTCATCC CTGCCTCCAT CGTGGGAATC ATTGTCTTCC TGTACGGATG CGCCACCATG
    GATGAAAACA TCCCCAGCAT GGAGATGTGT GACCAGAGAC ACAATATCAC CATGTGCCCG
    CTTTGCGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGCGCCAC GGCCCGCGCC
    AGCCACCTCT TCGACAACCC CGCCACGGTC TTCTTCTCTG TCTTCATGGC CCTCTGGGCT
    GCCACCTTCA TGGAGCACTG GAAGCGGAAA CAGATGCGAC TCAACTACCG CTGGGACCTC
    ACGGGCTTTG AAGAGGAAGA GGAGGCTGTC AAGGATCATC CTAGAGCTGA ATACGAAGCC
    AGAGTCTTGG AGAAGTCTCT GAAGAAAGAG TCCAGAAACA AAGAGAAGCG CCGGCATATT
    CCAGAGGAGT CAACAAACAA ATGGAAGCAG AGGGTTAAGA CAGCCATGGC GGGGGTGAAA
    TTGACTGACA AAGTGAAGCT GACATGGAGA GATCGGTTCC CAGCCTACCT CACTAACTTG
    GTCTCCATCA TCTTCATGAT TGCAGTGACG TTTGCCATCG TCCTCGGCGT CATCATCTAC
    AGAATCTCCA TGGCCGCCGC CTTGGCCATG AACTCCTCCC CCTCCGTGCG GTCCAACATC
    CGGGTCACAG TCACAGCCAC CGCAGTCATC ATCAACCTAG TGGTCATCAT CCTCCTGGAC
    GAGGTGTATG GCTGCATAGC CCGATGGCTC ACCAAGATCG AGGTCCCAAA GACGGAGAAA
    AGCTTTGAGG AGAGGCTGAT CTTCAAGGCT TTCCTGCTGA AGTTTGTGAA TTCCTACACC
    CCCATCTTTT ACGTGGCGTT CTTCAAAGGC CGGTTTGTTG GACGCCCGGG CGACTACGTG
    TACATTTTCC GTTCCTTCCG AATGGAAGAG TGTGCGCCAG GGGGCTGCCT GATGGAGCTA
    TGCATCCAGC TCAGCATCAT CATGCTGGGG AAACAGCTGA TCCAGAACAA CCTGTTCGAG
    ATCGGCATCC CGAAGATGAA GAAGCTCATC CGCTACCTGA AGCTGAAGCA GCAGAGCCCC
    CCTGACCACG AGGAGTGTGT GAAGAGGAAA CAGCGGTACG AGGTGGATTA CAACCTGGAG
    CCCTTCGCGG GCCTCACCCC AGAGTACATG GAAATGATCA TCCAGTTTGG CTTCGTCACC
    CTGTTTGTCG CCTCCTTCCC CCTGGCCCCA CTGTTTGCGC TGCTGAACAA CATCATCGAG
    ATCCGCCTGG ACGCCAAAAA GTTTGTCACT GAGCTCCGAA GGCCGGTAGC TGTCAGAGCC
    AAAGACATCG GAATCTGGTA CAATATCCTC AGAGGCATTG GGAAGCTTGC TGTCATCATC
    AATGCCTTCG TGATCTCCTT CACGTCTGAC TTCATCCCGC GCCTGGTGTA CCTCTACATG
    TACAGTAAGA ACGGGACCAT GCACGGCTTC GTCAACCACA CCCTCTCCTC CTTCAACGTC
    AGTGACTTCC AGAACGGCAC GGCCCCCAAT GACCCCCTGG ACCTGGGCTA CGAGGTGCAG
    ATCTGCAGGT ATAAAGACTA CCGAGAGCCG CCGTGGTCGG AAAACAAGTA CGACATCTCC
    AAGGACTTCT GGGCCGTCCT GGCAGCCCGG CTGGCGTTTG TCATCGTCTT CCAGAACCTG
    GTCATGTTCA TGAGCGACTT TGTGGACTGG GTCATCCCGG ACATCCCCAA GGACATCAGC
    CAGCAGATCC ACAAGGAGAA GGTGCTCATG GTGGAGCTGT TCATGCGGGA GGAGCAAGAC
    AAGCAGCAGC TGCTGGAAAC CTGGATGGAG AAGGAGCGGC AGAAGGACGA GCCGCCGTGC
    AACCACCACA ACACCAAAGC CTGCCCAGAC AGCCTCGGCA GCCCAGCCCC CAGCCATGCC
    TACCACGGGG GCGTCCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu68992
    Clone ID Related Accession (Same CDS sequence) XM_011545131.2
    Accession Version XM_011545131.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product anoctamin-1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545131.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCTGACCA GGCCCTCCCA GGTGCTGCTG AACTCCTTAT CTGTGGACCC TGATGCCGAG 
    TGCAAGTATG GCCTGTACTT CAGGGACGGC CGGCGCAAGG TGGACTACAT CCTGGTGTAC
    CATCACAAGA GGCCCTCGGG CAACCGGACC CTGGTCAGGA GGGTGCAGCA CAGCGACACC
    CCCTCTGGGG CTCGCAGCGT CAAGCAGGAC CACCCCCTGC CGGGCAAGGG GGCGTCGCTG
    GATGCAGGCT CGGGGGAGCC CCCGATGGAC TACCACGAGG ATGACAAGCG CTTCCGCAGG
    GAGGAGTACG AGGGCAACCT CCTGGAGGCG GGCCTGGAGC TGGAGCGGGA CGAGGACACT
    AAAATCCACG GAGTCGGGTT TGTGAAAATC CATGCCCCCT GGAACGTGCT GTGCAGAGAG
    GCCGAGTTTC TGAAACTGAA GATGCCGACG AAGAAGATGT ACCACATTAA TGAGACCCGT
    GGCCTCCTGA AAAAAATCAA CTCTGTGCTC CAGAAAATCA CAGATCCCAT CCAGCCCAAA
    GTGGCTGAGC ACAGGCCCCA GACCATGAAG AGACTCTCCT ATCCCTTCTC CCGGGAGAAG
    CAGCATCTAT TTGACTTGTC TGATAAGGAT TCCTTTTTCG ACAGCAAAAC CCGGAGCACG
    ATTGTCTATG AGATCTTGAA GAGAACGACG TGTACAAAGG CCAAGTACAG CATGGGCATC
    ACGAGCCTGC TGGCCAATGG TGTGTACGCG GCTGCATACC CACTGCACGA TGGAGACTAC
    AACGGTGAAA ACGTCGAGTT CAACGACAGA AAACTCCTGT ACGAAGAGTG GGCACGCTAT
    GGAGTTTTCT ATAAGTACCA GCCCATCGAC CTGGTCAGGA AGTATTTTGG GGAGAAGATC
    GGCCTGTACT TCGCCTGGCT GGGCGTGTAC ACCCAGATGC TCATCCCTGC CTCCATCGTG
    GGAATCATTG TCTTCCTGTA CGGATGCGCC ACCATGGATG AAAACATCCC CAGCATGGAG
    ATGTGTGACC AGAGACACAA TATCACCATG TGCCCGCTTT GCGACAAGAC CTGCAGCTAC
    TGGAAGATGA GCTCAGCCTG CGCCACGGCC CGCGCCAGCC ACCTCTTCGA CAACCCCGCC
    ACGGTCTTCT TCTCTGTCTT CATGGCCCTC TGGGCTGCCA CCTTCATGGA GCACTGGAAG
    CGGAAACAGA TGCGACTCAA CTACCGCTGG GACCTCACGG GCTTTGAAGA GGAAGAGGAT
    CATCCTAGAG CTGAATACGA AGCCAGAGTC TTGGAGAAGT CTCTGAAGAA AGAGTCCAGA
    AACAAAGAGA CTGACAAAGT GAAGCTGACA TGGAGAGATC GGTTCCCAGC CTACCTCACT
    AACTTGGTCT CCATCATCTT CATGATTGCA GTGACGTTTG CCATCGTCCT CGGCGTCATC
    ATCTACAGAA TCTCCATGGC CGCCGCCTTG GCCATGAACT CCTCCCCCTC CGTGCGGTCC
    AACATCCGGG TCACAGTCAC AGCCACCGCA GTCATCATCA ACCTAGTGGT CATCATCCTC
    CTGGACGAGG TGTATGGCTG CATAGCCCGA TGGCTCACCA AGATCGAGGT CCCAAAGACG
    GAGAAAAGCT TTGAGGAGAG GCTGATCTTC AAGGCTTTCC TGCTGAAGTT TGTGAATTCC
    TACACCCCCA TCTTTTACGT GGCGTTCTTC AAAGGCCGGT TTGTTGGACG CCCGGGCGAC
    TACGTGTACA TTTTCCGTTC CTTCCGAATG GAAGAGGTAA CCGAAATTTT ATTCATCTCT
    GGCAGCCCCT TCTGTCTTGC TTACGATTTG TCTACACCAT GCACTTGGGA GAAGCAGCTG
    CAGCACATTT GTTCAGCAAA GAGCAGCAGG TTTCTTTCCT TCTTGCTGGA AACTTTTCTG
    TTCCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011543433.1
    CDS106..2034
    Translation

    Target ORF information:

    RefSeq Version XM_011545131.2
    Organism Homo sapiens(human)
    Definition Homo sapiens anoctamin 1 (ANO1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011545131.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCTGACCA GGCCCTCCCA GGTGCTGCTG AACTCCTTAT CTGTGGACCC TGATGCCGAG 
    TGCAAGTATG GCCTGTACTT CAGGGACGGC CGGCGCAAGG TGGACTACAT CCTGGTGTAC
    CATCACAAGA GGCCCTCGGG CAACCGGACC CTGGTCAGGA GGGTGCAGCA CAGCGACACC
    CCCTCTGGGG CTCGCAGCGT CAAGCAGGAC CACCCCCTGC CGGGCAAGGG GGCGTCGCTG
    GATGCAGGCT CGGGGGAGCC CCCGATGGAC TACCACGAGG ATGACAAGCG CTTCCGCAGG
    GAGGAGTACG AGGGCAACCT CCTGGAGGCG GGCCTGGAGC TGGAGCGGGA CGAGGACACT
    AAAATCCACG GAGTCGGGTT TGTGAAAATC CATGCCCCCT GGAACGTGCT GTGCAGAGAG
    GCCGAGTTTC TGAAACTGAA GATGCCGACG AAGAAGATGT ACCACATTAA TGAGACCCGT
    GGCCTCCTGA AAAAAATCAA CTCTGTGCTC CAGAAAATCA CAGATCCCAT CCAGCCCAAA
    GTGGCTGAGC ACAGGCCCCA GACCATGAAG AGACTCTCCT ATCCCTTCTC CCGGGAGAAG
    CAGCATCTAT TTGACTTGTC TGATAAGGAT TCCTTTTTCG ACAGCAAAAC CCGGAGCACG
    ATTGTCTATG AGATCTTGAA GAGAACGACG TGTACAAAGG CCAAGTACAG CATGGGCATC
    ACGAGCCTGC TGGCCAATGG TGTGTACGCG GCTGCATACC CACTGCACGA TGGAGACTAC
    AACGGTGAAA ACGTCGAGTT CAACGACAGA AAACTCCTGT ACGAAGAGTG GGCACGCTAT
    GGAGTTTTCT ATAAGTACCA GCCCATCGAC CTGGTCAGGA AGTATTTTGG GGAGAAGATC
    GGCCTGTACT TCGCCTGGCT GGGCGTGTAC ACCCAGATGC TCATCCCTGC CTCCATCGTG
    GGAATCATTG TCTTCCTGTA CGGATGCGCC ACCATGGATG AAAACATCCC CAGCATGGAG
    ATGTGTGACC AGAGACACAA TATCACCATG TGCCCGCTTT GCGACAAGAC CTGCAGCTAC
    TGGAAGATGA GCTCAGCCTG CGCCACGGCC CGCGCCAGCC ACCTCTTCGA CAACCCCGCC
    ACGGTCTTCT TCTCTGTCTT CATGGCCCTC TGGGCTGCCA CCTTCATGGA GCACTGGAAG
    CGGAAACAGA TGCGACTCAA CTACCGCTGG GACCTCACGG GCTTTGAAGA GGAAGAGGAT
    CATCCTAGAG CTGAATACGA AGCCAGAGTC TTGGAGAAGT CTCTGAAGAA AGAGTCCAGA
    AACAAAGAGA CTGACAAAGT GAAGCTGACA TGGAGAGATC GGTTCCCAGC CTACCTCACT
    AACTTGGTCT CCATCATCTT CATGATTGCA GTGACGTTTG CCATCGTCCT CGGCGTCATC
    ATCTACAGAA TCTCCATGGC CGCCGCCTTG GCCATGAACT CCTCCCCCTC CGTGCGGTCC
    AACATCCGGG TCACAGTCAC AGCCACCGCA GTCATCATCA ACCTAGTGGT CATCATCCTC
    CTGGACGAGG TGTATGGCTG CATAGCCCGA TGGCTCACCA AGATCGAGGT CCCAAAGACG
    GAGAAAAGCT TTGAGGAGAG GCTGATCTTC AAGGCTTTCC TGCTGAAGTT TGTGAATTCC
    TACACCCCCA TCTTTTACGT GGCGTTCTTC AAAGGCCGGT TTGTTGGACG CCCGGGCGAC
    TACGTGTACA TTTTCCGTTC CTTCCGAATG GAAGAGGTAA CCGAAATTTT ATTCATCTCT
    GGCAGCCCCT TCTGTCTTGC TTACGATTTG TCTACACCAT GCACTTGGGA GAAGCAGCTG
    CAGCACATTT GTTCAGCAAA GAGCAGCAGG TTTCTTTCCT TCTTGCTGGA AACTTTTCTG
    TTCCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45395
    Clone ID Related Accession (Same CDS sequence) XM_006718602.2
    Accession Version XM_006718602.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2943bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product anoctamin-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006718602.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCTGACCA GGCCCTCCCA GGTGCTGCTG AACTCCTTAT CTGTGGACCC TGATGCCGAG 
    TGCAAGTATG GCCTGTACTT CAGGGACGGC CGGCGCAAGG TGGACTACAT CCTGGTGTAC
    CATCACAAGA GGCCCTCGGG CAACCGGACC CTGGTCAGGA GGGTGCAGCA CAGCGACACC
    CCCTCTGGGG CTCGCAGCGT CAAGCAGGAC CACCCCCTGC CGGGCAAGGG GGCGTCGCTG
    GATGCAGGCT CGGGGGAGCC CCCGATGGAC TACCACGAGG ATGACAAGCG CTTCCGCAGG
    GAGGAGTACG AGGGCAACCT CCTGGAGGCG GGCCTGGAGC TGGAGCGGGA CGAGGACACT
    AAAATCCACG GAGTCGGGTT TGTGAAAATC CATGCCCCCT GGAACGTGCT GTGCAGAGAG
    GCCGAGTTTC TGAAACTGAA GATGCCGACG AAGAAGATGT ACCACATTAA TGAGACCCGT
    GGCCTCCTGA AAAAAATCAA CTCTGTGCTC CAGAAAATCA CAGATCCCAT CCAGCCCAAA
    GTGGCTGAGC ACAGGCCCCA GACCATGAAG AGACTCTCCT ATCCCTTCTC CCGGGAGAAG
    CAGCATCTAT TTGACTTGTC TGATAAGGAT TCCTTTTTCG ACAGCAAAAC CCGGAGCACG
    ATTGTCTATG AGATCTTGAA GAGAACGACG TGTACAAAGG CCAAGTACAG CATGGGGCAA
    GGCGAGGGAA GAAAGAAGGA CTCCGCCCTT CTAAGTAAAA GGCGGAAATG TGGGAAGTAT
    GGCATCACGA GCCTGCTGGC CAATGGTGTG TACGCGGCTG CATACCCACT GCACGATGGA
    GACTACAACG GTGAAAACGT CGAGTTCAAC GACAGAAAAC TCCTGTACGA AGAGTGGGCA
    CGCTATGGAG TTTTCTATAA GTACCAGCCC ATCGACCTGG TCAGGAAGTA TTTTGGGGAG
    AAGATCGGCC TGTACTTCGC CTGGCTGGGC GTGTACACCC AGATGCTCAT CCCTGCCTCC
    ATCGTGGGAA TCATTGTCTT CCTGTACGGA TGCGCCACCA TGGATGAAAA CATCCCCAGC
    ATGGAGATGT GTGACCAGAG ACACAATATC ACCATGTGCC CGCTTTGCGA CAAGACCTGC
    AGCTACTGGA AGATGAGCTC AGCCTGCGCC ACGGCCCGCG CCAGCCACCT CTTCGACAAC
    CCCGCCACGG TCTTCTTCTC TGTCTTCATG GCCCTCTGGG CTGCCACCTT CATGGAGCAC
    TGGAAGCGGA AACAGATGCG ACTCAACTAC CGCTGGGACC TCACGGGCTT TGAAGAGGAA
    GAGGAGGCTG TCAAGGATCA TCCTAGAGCT GAATACGAAG CCAGAGTCTT GGAGAAGTCT
    CTGAAGAAAG AGTCCAGAAA CAAAGAGAAG CGCCGGCATA TTCCAGAGGA GTCAACAAAC
    AAATGGAAGC AGAGGGTTAA GACAGCCATG GCGGGGGTGA AATTGACTGA CAAAGTGAAG
    CTGACATGGA GAGATCGGTT CCCAGCCTAC CTCACTAACT TGGTCTCCAT CATCTTCATG
    ATTGCAGTGA CGTTTGCCAT CGTCCTCGGC GTCATCATCT ACAGAATCTC CATGGCCGCC
    GCCTTGGCCA TGAACTCCTC CCCCTCCGTG CGGTCCAACA TCCGGGTCAC AGTCACAGCC
    ACCGCAGTCA TCATCAACCT AGTGGTCATC ATCCTCCTGG ACGAGGTGTA TGGCTGCATA
    GCCCGATGGC TCACCAAGAT CGAGGTCCCA AAGACGGAGA AAAGCTTTGA GGAGAGGCTG
    ATCTTCAAGG CTTTCCTGCT GAAGTTTGTG AATTCCTACA CCCCCATCTT TTACGTGGCG
    TTCTTCAAAG GCCGGTTTGT TGGACGCCCG GGCGACTACG TGTACATTTT CCGTTCCTTC
    CGAATGGAAG AGTGTGCGCC AGGGGGCTGC CTGATGGAGC TATGCATCCA GCTCAGCATC
    ATCATGCTGG GGAAACAGCT GATCCAGAAC AACCTGTTCG AGATCGGCAT CCCGAAGATG
    AAGAAGCTCA TCCGCTACCT GAAGCTGAAG CAGCAGAGCC CCCCTGACCA CGAGGAGTGT
    GTGAAGAGGA AACAGCGGTA CGAGGTGGAT TACAACCTGG AGCCCTTCGC GGGCCTCACC
    CCAGAGTACA TGGAAATGAT CATCCAGTTT GGCTTCGTCA CCCTGTTTGT CGCCTCCTTC
    CCCCTGGCCC CACTGTTTGC GCTGCTGAAC AACATCATCG AGATCCGCCT GGACGCCAAA
    AAGTTTGTCA CTGAGCTCCG AAGGCCGGTA GCTGTCAGAG CCAAAGACAT CGGAATCTGG
    TACAATATCC TCAGAGGCAT TGGGAAGCTT GCTGTCATCA TCAATGCCTT CGTGATCTCC
    TTCACGTCTG ACTTCATCCC GCGCCTGGTG TACCTCTACA TGTACAGTAA GAACGGGACC
    ATGCACGGCT TCGTCAACCA CACCCTCTCC TCCTTCAACG TCAGTGACTT CCAGAACGGC
    ACGGCCCCCA ATGACCCCCT GGACCTGGGC TACGAGGTGC AGATCTGCAG GTATAAAGAC
    TACCGAGAGC CGCCGTGGTC GGAAAACAAG TACGACATCT CCAAGGACTT CTGGGCCGTC
    CTGGCAGCCC GGCTGGCGTT TGTCATCGTC TTCCAGAACC TGGTCATGTT CATGAGCGAC
    TTTGTGGACT GGGTCATCCC GGACATCCCC AAGGACATCA GCCAGCAGAT CCACAAGGAG
    AAGGTGCTCA TGGTGGAGCT GTTCATGCGG GAGGAGCAAG ACAAGCAGCA GCTGCTGGAA
    ACCTGGATGG AGAAGGAGCG GCAGAAGGAC GAGCCGCCGT GCAACCACCA CAACACCAAA
    GCCTGCCCAG ACAGCCTCGG CAGCCCAGCC CCCAGCCATG CCTACCACGG GGGCGTCCTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006718665.1
    CDS106..3048
    Translation

    Target ORF information:

    RefSeq Version XM_006718602.2
    Organism Homo sapiens(human)
    Definition Homo sapiens anoctamin 1 (ANO1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006718602.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCTGACCA GGCCCTCCCA GGTGCTGCTG AACTCCTTAT CTGTGGACCC TGATGCCGAG 
    TGCAAGTATG GCCTGTACTT CAGGGACGGC CGGCGCAAGG TGGACTACAT CCTGGTGTAC
    CATCACAAGA GGCCCTCGGG CAACCGGACC CTGGTCAGGA GGGTGCAGCA CAGCGACACC
    CCCTCTGGGG CTCGCAGCGT CAAGCAGGAC CACCCCCTGC CGGGCAAGGG GGCGTCGCTG
    GATGCAGGCT CGGGGGAGCC CCCGATGGAC TACCACGAGG ATGACAAGCG CTTCCGCAGG
    GAGGAGTACG AGGGCAACCT CCTGGAGGCG GGCCTGGAGC TGGAGCGGGA CGAGGACACT
    AAAATCCACG GAGTCGGGTT TGTGAAAATC CATGCCCCCT GGAACGTGCT GTGCAGAGAG
    GCCGAGTTTC TGAAACTGAA GATGCCGACG AAGAAGATGT ACCACATTAA TGAGACCCGT
    GGCCTCCTGA AAAAAATCAA CTCTGTGCTC CAGAAAATCA CAGATCCCAT CCAGCCCAAA
    GTGGCTGAGC ACAGGCCCCA GACCATGAAG AGACTCTCCT ATCCCTTCTC CCGGGAGAAG
    CAGCATCTAT TTGACTTGTC TGATAAGGAT TCCTTTTTCG ACAGCAAAAC CCGGAGCACG
    ATTGTCTATG AGATCTTGAA GAGAACGACG TGTACAAAGG CCAAGTACAG CATGGGGCAA
    GGCGAGGGAA GAAAGAAGGA CTCCGCCCTT CTAAGTAAAA GGCGGAAATG TGGGAAGTAT
    GGCATCACGA GCCTGCTGGC CAATGGTGTG TACGCGGCTG CATACCCACT GCACGATGGA
    GACTACAACG GTGAAAACGT CGAGTTCAAC GACAGAAAAC TCCTGTACGA AGAGTGGGCA
    CGCTATGGAG TTTTCTATAA GTACCAGCCC ATCGACCTGG TCAGGAAGTA TTTTGGGGAG
    AAGATCGGCC TGTACTTCGC CTGGCTGGGC GTGTACACCC AGATGCTCAT CCCTGCCTCC
    ATCGTGGGAA TCATTGTCTT CCTGTACGGA TGCGCCACCA TGGATGAAAA CATCCCCAGC
    ATGGAGATGT GTGACCAGAG ACACAATATC ACCATGTGCC CGCTTTGCGA CAAGACCTGC
    AGCTACTGGA AGATGAGCTC AGCCTGCGCC ACGGCCCGCG CCAGCCACCT CTTCGACAAC
    CCCGCCACGG TCTTCTTCTC TGTCTTCATG GCCCTCTGGG CTGCCACCTT CATGGAGCAC
    TGGAAGCGGA AACAGATGCG ACTCAACTAC CGCTGGGACC TCACGGGCTT TGAAGAGGAA
    GAGGAGGCTG TCAAGGATCA TCCTAGAGCT GAATACGAAG CCAGAGTCTT GGAGAAGTCT
    CTGAAGAAAG AGTCCAGAAA CAAAGAGAAG CGCCGGCATA TTCCAGAGGA GTCAACAAAC
    AAATGGAAGC AGAGGGTTAA GACAGCCATG GCGGGGGTGA AATTGACTGA CAAAGTGAAG
    CTGACATGGA GAGATCGGTT CCCAGCCTAC CTCACTAACT TGGTCTCCAT CATCTTCATG
    ATTGCAGTGA CGTTTGCCAT CGTCCTCGGC GTCATCATCT ACAGAATCTC CATGGCCGCC
    GCCTTGGCCA TGAACTCCTC CCCCTCCGTG CGGTCCAACA TCCGGGTCAC AGTCACAGCC
    ACCGCAGTCA TCATCAACCT AGTGGTCATC ATCCTCCTGG ACGAGGTGTA TGGCTGCATA
    GCCCGATGGC TCACCAAGAT CGAGGTCCCA AAGACGGAGA AAAGCTTTGA GGAGAGGCTG
    ATCTTCAAGG CTTTCCTGCT GAAGTTTGTG AATTCCTACA CCCCCATCTT TTACGTGGCG
    TTCTTCAAAG GCCGGTTTGT TGGACGCCCG GGCGACTACG TGTACATTTT CCGTTCCTTC
    CGAATGGAAG AGTGTGCGCC AGGGGGCTGC CTGATGGAGC TATGCATCCA GCTCAGCATC
    ATCATGCTGG GGAAACAGCT GATCCAGAAC AACCTGTTCG AGATCGGCAT CCCGAAGATG
    AAGAAGCTCA TCCGCTACCT GAAGCTGAAG CAGCAGAGCC CCCCTGACCA CGAGGAGTGT
    GTGAAGAGGA AACAGCGGTA CGAGGTGGAT TACAACCTGG AGCCCTTCGC GGGCCTCACC
    CCAGAGTACA TGGAAATGAT CATCCAGTTT GGCTTCGTCA CCCTGTTTGT CGCCTCCTTC
    CCCCTGGCCC CACTGTTTGC GCTGCTGAAC AACATCATCG AGATCCGCCT GGACGCCAAA
    AAGTTTGTCA CTGAGCTCCG AAGGCCGGTA GCTGTCAGAG CCAAAGACAT CGGAATCTGG
    TACAATATCC TCAGAGGCAT TGGGAAGCTT GCTGTCATCA TCAATGCCTT CGTGATCTCC
    TTCACGTCTG ACTTCATCCC GCGCCTGGTG TACCTCTACA TGTACAGTAA GAACGGGACC
    ATGCACGGCT TCGTCAACCA CACCCTCTCC TCCTTCAACG TCAGTGACTT CCAGAACGGC
    ACGGCCCCCA ATGACCCCCT GGACCTGGGC TACGAGGTGC AGATCTGCAG GTATAAAGAC
    TACCGAGAGC CGCCGTGGTC GGAAAACAAG TACGACATCT CCAAGGACTT CTGGGCCGTC
    CTGGCAGCCC GGCTGGCGTT TGTCATCGTC TTCCAGAACC TGGTCATGTT CATGAGCGAC
    TTTGTGGACT GGGTCATCCC GGACATCCCC AAGGACATCA GCCAGCAGAT CCACAAGGAG
    AAGGTGCTCA TGGTGGAGCT GTTCATGCGG GAGGAGCAAG ACAAGCAGCA GCTGCTGGAA
    ACCTGGATGG AGAAGGAGCG GCAGAAGGAC GAGCCGCCGT GCAACCACCA CAACACCAAA
    GCCTGCCCAG ACAGCCTCGG CAGCCCAGCC CCCAGCCATG CCTACCACGG GGGCGTCCTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45397
    Clone ID Related Accession (Same CDS sequence) XM_006718604.2
    Accession Version XM_006718604.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2679bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product anoctamin-1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006718604.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACTACC ACGAGGATGA CAAGCGCTTC CGCAGGGAGG AGTACGAGGG CAACCTCCTG 
    GAGGCGGGCC TGGAGCTGGA GCGGGACGAG GACACTAAAA TCCACGGAGT CGGGTTTGTG
    AAAATCCATG CCCCCTGGAA CGTGCTGTGC AGAGAGGCCG AGTTTCTGAA ACTGAAGATG
    CCGACGAAGA AGATGTACCA CATTAATGAG ACCCGTGGCC TCCTGAAAAA AATCAACTCT
    GTGCTCCAGA AAATCACAGA TCCCATCCAG CCCAAAGTGG CTGAGCACAG GCCCCAGACC
    ATGAAGAGAC TCTCCTATCC CTTCTCCCGG GAGAAGCAGC ATCTATTTGA CTTGTCTGAT
    AAGGATTCCT TTTTCGACAG CAAAACCCGG AGCACGATTG TCTATGAGAT CTTGAAGAGA
    ACGACGTGTA CAAAGGCCAA GTACAGCATG GGGCAAGGCG AGGGAAGAAA GAAGGACTCC
    GCCCTTCTAA GTAAAAGGCG GAAATGTGGG AAGTATGGCA TCACGAGCCT GCTGGCCAAT
    GGTGTGTACG CGGCTGCATA CCCACTGCAC GATGGAGACT ACAACGGTGA AAACGTCGAG
    TTCAACGACA GAAAACTCCT GTACGAAGAG TGGGCACGCT ATGGAGTTTT CTATAAGTAC
    CAGCCCATCG ACCTGGTCAG GAAGTATTTT GGGGAGAAGA TCGGCCTGTA CTTCGCCTGG
    CTGGGCGTGT ACACCCAGAT GCTCATCCCT GCCTCCATCG TGGGAATCAT TGTCTTCCTG
    TACGGATGCG CCACCATGGA TGAAAACATC CCCAGCATGG AGATGTGTGA CCAGAGACAC
    AATATCACCA TGTGCCCGCT TTGCGACAAG ACCTGCAGCT ACTGGAAGAT GAGCTCAGCC
    TGCGCCACGG CCCGCGCCAG CCACCTCTTC GACAACCCCG CCACGGTCTT CTTCTCTGTC
    TTCATGGCCC TCTGGGCTGC CACCTTCATG GAGCACTGGA AGCGGAAACA GATGCGACTC
    AACTACCGCT GGGACCTCAC GGGCTTTGAA GAGGAAGAGG AGGCTGTCAA GGATCATCCT
    AGAGCTGAAT ACGAAGCCAG AGTCTTGGAG AAGTCTCTGA AGAAAGAGTC CAGAAACAAA
    GAGAAGCGCC GGCATATTCC AGAGGAGTCA ACAAACAAAT GGAAGCAGAG GGTTAAGACA
    GCCATGGCGG GGGTGAAATT GACTGACAAA GTGAAGCTGA CATGGAGAGA TCGGTTCCCA
    GCCTACCTCA CTAACTTGGT CTCCATCATC TTCATGATTG CAGTGACGTT TGCCATCGTC
    CTCGGCGTCA TCATCTACAG AATCTCCATG GCCGCCGCCT TGGCCATGAA CTCCTCCCCC
    TCCGTGCGGT CCAACATCCG GGTCACAGTC ACAGCCACCG CAGTCATCAT CAACCTAGTG
    GTCATCATCC TCCTGGACGA GGTGTATGGC TGCATAGCCC GATGGCTCAC CAAGATCGAG
    GTCCCAAAGA CGGAGAAAAG CTTTGAGGAG AGGCTGATCT TCAAGGCTTT CCTGCTGAAG
    TTTGTGAATT CCTACACCCC CATCTTTTAC GTGGCGTTCT TCAAAGGCCG GTTTGTTGGA
    CGCCCGGGCG ACTACGTGTA CATTTTCCGT TCCTTCCGAA TGGAAGAGTG TGCGCCAGGG
    GGCTGCCTGA TGGAGCTATG CATCCAGCTC AGCATCATCA TGCTGGGGAA ACAGCTGATC
    CAGAACAACC TGTTCGAGAT CGGCATCCCG AAGATGAAGA AGCTCATCCG CTACCTGAAG
    CTGAAGCAGC AGAGCCCCCC TGACCACGAG GAGTGTGTGA AGAGGAAACA GCGGTACGAG
    GTGGATTACA ACCTGGAGCC CTTCGCGGGC CTCACCCCAG AGTACATGGA AATGATCATC
    CAGTTTGGCT TCGTCACCCT GTTTGTCGCC TCCTTCCCCC TGGCCCCACT GTTTGCGCTG
    CTGAACAACA TCATCGAGAT CCGCCTGGAC GCCAAAAAGT TTGTCACTGA GCTCCGAAGG
    CCGGTAGCTG TCAGAGCCAA AGACATCGGA ATCTGGTACA ATATCCTCAG AGGCATTGGG
    AAGCTTGCTG TCATCATCAA TGCCTTCGTG ATCTCCTTCA CGTCTGACTT CATCCCGCGC
    CTGGTGTACC TCTACATGTA CAGTAAGAAC GGGACCATGC ACGGCTTCGT CAACCACACC
    CTCTCCTCCT TCAACGTCAG TGACTTCCAG AACGGCACGG CCCCCAATGA CCCCCTGGAC
    CTGGGCTACG AGGTGCAGAT CTGCAGGTAT AAAGACTACC GAGAGCCGCC GTGGTCGGAA
    AACAAGTACG ACATCTCCAA GGACTTCTGG GCCGTCCTGG CAGCCCGGCT GGCGTTTGTC
    ATCGTCTTCC AGAACCTGGT CATGTTCATG AGCGACTTTG TGGACTGGGT CATCCCGGAC
    ATCCCCAAGG ACATCAGCCA GCAGATCCAC AAGGAGAAGG TGCTCATGGT GGAGCTGTTC
    ATGCGGGAGG AGCAAGACAA GCAGCAGCTG CTGGAAACCT GGATGGAGAA GGAGCGGCAG
    AAGGACGAGC CGCCGTGCAA CCACCACAAC ACCAAAGCCT GCCCAGACAG CCTCGGCAGC
    CCAGCCCCCA GCCATGCCTA CCACGGGGGC GTCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006718667.1
    CDS485..3163
    Translation

    Target ORF information:

    RefSeq Version XM_006718604.2
    Organism Homo sapiens(human)
    Definition Homo sapiens anoctamin 1 (ANO1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006718604.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACTACC ACGAGGATGA CAAGCGCTTC CGCAGGGAGG AGTACGAGGG CAACCTCCTG 
    GAGGCGGGCC TGGAGCTGGA GCGGGACGAG GACACTAAAA TCCACGGAGT CGGGTTTGTG
    AAAATCCATG CCCCCTGGAA CGTGCTGTGC AGAGAGGCCG AGTTTCTGAA ACTGAAGATG
    CCGACGAAGA AGATGTACCA CATTAATGAG ACCCGTGGCC TCCTGAAAAA AATCAACTCT
    GTGCTCCAGA AAATCACAGA TCCCATCCAG CCCAAAGTGG CTGAGCACAG GCCCCAGACC
    ATGAAGAGAC TCTCCTATCC CTTCTCCCGG GAGAAGCAGC ATCTATTTGA CTTGTCTGAT
    AAGGATTCCT TTTTCGACAG CAAAACCCGG AGCACGATTG TCTATGAGAT CTTGAAGAGA
    ACGACGTGTA CAAAGGCCAA GTACAGCATG GGGCAAGGCG AGGGAAGAAA GAAGGACTCC
    GCCCTTCTAA GTAAAAGGCG GAAATGTGGG AAGTATGGCA TCACGAGCCT GCTGGCCAAT
    GGTGTGTACG CGGCTGCATA CCCACTGCAC GATGGAGACT ACAACGGTGA AAACGTCGAG
    TTCAACGACA GAAAACTCCT GTACGAAGAG TGGGCACGCT ATGGAGTTTT CTATAAGTAC
    CAGCCCATCG ACCTGGTCAG GAAGTATTTT GGGGAGAAGA TCGGCCTGTA CTTCGCCTGG
    CTGGGCGTGT ACACCCAGAT GCTCATCCCT GCCTCCATCG TGGGAATCAT TGTCTTCCTG
    TACGGATGCG CCACCATGGA TGAAAACATC CCCAGCATGG AGATGTGTGA CCAGAGACAC
    AATATCACCA TGTGCCCGCT TTGCGACAAG ACCTGCAGCT ACTGGAAGAT GAGCTCAGCC
    TGCGCCACGG CCCGCGCCAG CCACCTCTTC GACAACCCCG CCACGGTCTT CTTCTCTGTC
    TTCATGGCCC TCTGGGCTGC CACCTTCATG GAGCACTGGA AGCGGAAACA GATGCGACTC
    AACTACCGCT GGGACCTCAC GGGCTTTGAA GAGGAAGAGG AGGCTGTCAA GGATCATCCT
    AGAGCTGAAT ACGAAGCCAG AGTCTTGGAG AAGTCTCTGA AGAAAGAGTC CAGAAACAAA
    GAGAAGCGCC GGCATATTCC AGAGGAGTCA ACAAACAAAT GGAAGCAGAG GGTTAAGACA
    GCCATGGCGG GGGTGAAATT GACTGACAAA GTGAAGCTGA CATGGAGAGA TCGGTTCCCA
    GCCTACCTCA CTAACTTGGT CTCCATCATC TTCATGATTG CAGTGACGTT TGCCATCGTC
    CTCGGCGTCA TCATCTACAG AATCTCCATG GCCGCCGCCT TGGCCATGAA CTCCTCCCCC
    TCCGTGCGGT CCAACATCCG GGTCACAGTC ACAGCCACCG CAGTCATCAT CAACCTAGTG
    GTCATCATCC TCCTGGACGA GGTGTATGGC TGCATAGCCC GATGGCTCAC CAAGATCGAG
    GTCCCAAAGA CGGAGAAAAG CTTTGAGGAG AGGCTGATCT TCAAGGCTTT CCTGCTGAAG
    TTTGTGAATT CCTACACCCC CATCTTTTAC GTGGCGTTCT TCAAAGGCCG GTTTGTTGGA
    CGCCCGGGCG ACTACGTGTA CATTTTCCGT TCCTTCCGAA TGGAAGAGTG TGCGCCAGGG
    GGCTGCCTGA TGGAGCTATG CATCCAGCTC AGCATCATCA TGCTGGGGAA ACAGCTGATC
    CAGAACAACC TGTTCGAGAT CGGCATCCCG AAGATGAAGA AGCTCATCCG CTACCTGAAG
    CTGAAGCAGC AGAGCCCCCC TGACCACGAG GAGTGTGTGA AGAGGAAACA GCGGTACGAG
    GTGGATTACA ACCTGGAGCC CTTCGCGGGC CTCACCCCAG AGTACATGGA AATGATCATC
    CAGTTTGGCT TCGTCACCCT GTTTGTCGCC TCCTTCCCCC TGGCCCCACT GTTTGCGCTG
    CTGAACAACA TCATCGAGAT CCGCCTGGAC GCCAAAAAGT TTGTCACTGA GCTCCGAAGG
    CCGGTAGCTG TCAGAGCCAA AGACATCGGA ATCTGGTACA ATATCCTCAG AGGCATTGGG
    AAGCTTGCTG TCATCATCAA TGCCTTCGTG ATCTCCTTCA CGTCTGACTT CATCCCGCGC
    CTGGTGTACC TCTACATGTA CAGTAAGAAC GGGACCATGC ACGGCTTCGT CAACCACACC
    CTCTCCTCCT TCAACGTCAG TGACTTCCAG AACGGCACGG CCCCCAATGA CCCCCTGGAC
    CTGGGCTACG AGGTGCAGAT CTGCAGGTAT AAAGACTACC GAGAGCCGCC GTGGTCGGAA
    AACAAGTACG ACATCTCCAA GGACTTCTGG GCCGTCCTGG CAGCCCGGCT GGCGTTTGTC
    ATCGTCTTCC AGAACCTGGT CATGTTCATG AGCGACTTTG TGGACTGGGT CATCCCGGAC
    ATCCCCAAGG ACATCAGCCA GCAGATCCAC AAGGAGAAGG TGCTCATGGT GGAGCTGTTC
    ATGCGGGAGG AGCAAGACAA GCAGCAGCTG CTGGAAACCT GGATGGAGAA GGAGCGGCAG
    AAGGACGAGC CGCCGTGCAA CCACCACAAC ACCAAAGCCT GCCCAGACAG CCTCGGCAGC
    CCAGCCCCCA GCCATGCCTA CCACGGGGGC GTCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45398
    Clone ID Related Accession (Same CDS sequence) XM_006718605.2
    Accession Version XM_006718605.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2523bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product anoctamin-1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006718605.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGACTACC ACGAGGATGA CAAGCGCTTC CGCAGGGAGG AGTACGAGGG CAACCTCCTG 
    GAGGCGGGCC TGGAGCTGGA GCGGGACGAG GACACTAAAA TCCACGGAGT CGGGTTTGTG
    AAAATCCATG CCCCCTGGAA CGTGCTGTGC AGAGAGGCCG AGTTTCTGAA ACTGAAGATG
    CCGACGAAGA AGATGTACCA CATTAATGAG ACCCGTGGCC TCCTGAAAAA AATCAACTCT
    GTGCTCCAGA AAATCACAGA TCCCATCCAG CCCAAAGTGG CTGAGCACAG GCCCCAGACC
    ATGAAGAGAC TCTCCTATCC CTTCTCCCGG GAGAAGCAGC ATCTATTTGA CTTGTCTGAT
    AAGGATTCCT TTTTCGACAG CAAAACCCGG AGCACGATTG TCTATGAGAT CTTGAAGAGA
    ACGACGTGTA CAAAGGCCAA GTACAGCATG GGCATCACGA GCCTGCTGGC CAATGGTGTG
    TACGCGGCTG CATACCCACT GCACGATGGA GACTACAACG GTGAAAACGT CGAGTTCAAC
    GACAGAAAAC TCCTGTACGA AGAGTGGGCA CGCTATGGAG TTTTCTATAA GTACCAGCCC
    ATCGACCTGG TCAGGAAGTA TTTTGGGGAG AAGATCGGCC TGTACTTCGC CTGGCTGGGC
    GTGTACACCC AGATGCTCAT CCCTGCCTCC ATCGTGGGAA TCATTGTCTT CCTGTACGGA
    TGCGCCACCA TGGATGAAAA CATCCCCAGC ATGGAGATGT GTGACCAGAG ACACAATATC
    ACCATGTGCC CGCTTTGCGA CAAGACCTGC AGCTACTGGA AGATGAGCTC AGCCTGCGCC
    ACGGCCCGCG CCAGCCACCT CTTCGACAAC CCCGCCACGG TCTTCTTCTC TGTCTTCATG
    GCCCTCTGGG CTGCCACCTT CATGGAGCAC TGGAAGCGGA AACAGATGCG ACTCAACTAC
    CGCTGGGACC TCACGGGCTT TGAAGAGGAA GAGGATCATC CTAGAGCTGA ATACGAAGCC
    AGAGTCTTGG AGAAGTCTCT GAAGAAAGAG TCCAGAAACA AAGAGACTGA CAAAGTGAAG
    CTGACATGGA GAGATCGGTT CCCAGCCTAC CTCACTAACT TGGTCTCCAT CATCTTCATG
    ATTGCAGTGA CGTTTGCCAT CGTCCTCGGC GTCATCATCT ACAGAATCTC CATGGCCGCC
    GCCTTGGCCA TGAACTCCTC CCCCTCCGTG CGGTCCAACA TCCGGGTCAC AGTCACAGCC
    ACCGCAGTCA TCATCAACCT AGTGGTCATC ATCCTCCTGG ACGAGGTGTA TGGCTGCATA
    GCCCGATGGC TCACCAAGAT CGAGGTCCCA AAGACGGAGA AAAGCTTTGA GGAGAGGCTG
    ATCTTCAAGG CTTTCCTGCT GAAGTTTGTG AATTCCTACA CCCCCATCTT TTACGTGGCG
    TTCTTCAAAG GCCGGTTTGT TGGACGCCCG GGCGACTACG TGTACATTTT CCGTTCCTTC
    CGAATGGAAG AGTGTGCGCC AGGGGGCTGC CTGATGGAGC TATGCATCCA GCTCAGCATC
    ATCATGCTGG GGAAACAGCT GATCCAGAAC AACCTGTTCG AGATCGGCAT CCCGAAGATG
    AAGAAGCTCA TCCGCTACCT GAAGCTGAAG CAGCAGAGCC CCCCTGACCA CGAGGAGTGT
    GTGAAGAGGA AACAGCGGTA CGAGGTGGAT TACAACCTGG AGCCCTTCGC GGGCCTCACC
    CCAGAGTACA TGGAAATGAT CATCCAGTTT GGCTTCGTCA CCCTGTTTGT CGCCTCCTTC
    CCCCTGGCCC CACTGTTTGC GCTGCTGAAC AACATCATCG AGATCCGCCT GGACGCCAAA
    AAGTTTGTCA CTGAGCTCCG AAGGCCGGTA GCTGTCAGAG CCAAAGACAT CGGAATCTGG
    TACAATATCC TCAGAGGCAT TGGGAAGCTT GCTGTCATCA TCAATGCCTT CGTGATCTCC
    TTCACGTCTG ACTTCATCCC GCGCCTGGTG TACCTCTACA TGTACAGTAA GAACGGGACC
    ATGCACGGCT TCGTCAACCA CACCCTCTCC TCCTTCAACG TCAGTGACTT CCAGAACGGC
    ACGGCCCCCA ATGACCCCCT GGACCTGGGC TACGAGGTGC AGATCTGCAG GTATAAAGAC
    TACCGAGAGC CGCCGTGGTC GGAAAACAAG TACGACATCT CCAAGGACTT CTGGGCCGTC
    CTGGCAGCCC GGCTGGCGTT TGTCATCGTC TTCCAGAACC TGGTCATGTT CATGAGCGAC
    TTTGTGGACT GGGTCATCCC GGACATCCCC AAGGACATCA GCCAGCAGAT CCACAAGGAG
    AAGGTGCTCA TGGTGGAGCT GTTCATGCGG GAGGAGCAAG ACAAGCAGCA GCTGCTGGAA
    ACCTGGATGG AGAAGGAGCG GCAGAAGGAC GAGCCGCCGT GCAACCACCA CAACACCAAA
    GCCTGCCCAG ACAGCCTCGG CAGCCCAGCC CCCAGCCATG CCTACCACGG GGGCGTCCTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006718668.1
    CDS497..3019
    Translation

    Target ORF information:

    RefSeq Version XM_006718605.2
    Organism Homo sapiens(human)
    Definition Homo sapiens anoctamin 1 (ANO1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006718605.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGACTACC ACGAGGATGA CAAGCGCTTC CGCAGGGAGG AGTACGAGGG CAACCTCCTG 
    GAGGCGGGCC TGGAGCTGGA GCGGGACGAG GACACTAAAA TCCACGGAGT CGGGTTTGTG
    AAAATCCATG CCCCCTGGAA CGTGCTGTGC AGAGAGGCCG AGTTTCTGAA ACTGAAGATG
    CCGACGAAGA AGATGTACCA CATTAATGAG ACCCGTGGCC TCCTGAAAAA AATCAACTCT
    GTGCTCCAGA AAATCACAGA TCCCATCCAG CCCAAAGTGG CTGAGCACAG GCCCCAGACC
    ATGAAGAGAC TCTCCTATCC CTTCTCCCGG GAGAAGCAGC ATCTATTTGA CTTGTCTGAT
    AAGGATTCCT TTTTCGACAG CAAAACCCGG AGCACGATTG TCTATGAGAT CTTGAAGAGA
    ACGACGTGTA CAAAGGCCAA GTACAGCATG GGCATCACGA GCCTGCTGGC CAATGGTGTG
    TACGCGGCTG CATACCCACT GCACGATGGA GACTACAACG GTGAAAACGT CGAGTTCAAC
    GACAGAAAAC TCCTGTACGA AGAGTGGGCA CGCTATGGAG TTTTCTATAA GTACCAGCCC
    ATCGACCTGG TCAGGAAGTA TTTTGGGGAG AAGATCGGCC TGTACTTCGC CTGGCTGGGC
    GTGTACACCC AGATGCTCAT CCCTGCCTCC ATCGTGGGAA TCATTGTCTT CCTGTACGGA
    TGCGCCACCA TGGATGAAAA CATCCCCAGC ATGGAGATGT GTGACCAGAG ACACAATATC
    ACCATGTGCC CGCTTTGCGA CAAGACCTGC AGCTACTGGA AGATGAGCTC AGCCTGCGCC
    ACGGCCCGCG CCAGCCACCT CTTCGACAAC CCCGCCACGG TCTTCTTCTC TGTCTTCATG
    GCCCTCTGGG CTGCCACCTT CATGGAGCAC TGGAAGCGGA AACAGATGCG ACTCAACTAC
    CGCTGGGACC TCACGGGCTT TGAAGAGGAA GAGGATCATC CTAGAGCTGA ATACGAAGCC
    AGAGTCTTGG AGAAGTCTCT GAAGAAAGAG TCCAGAAACA AAGAGACTGA CAAAGTGAAG
    CTGACATGGA GAGATCGGTT CCCAGCCTAC CTCACTAACT TGGTCTCCAT CATCTTCATG
    ATTGCAGTGA CGTTTGCCAT CGTCCTCGGC GTCATCATCT ACAGAATCTC CATGGCCGCC
    GCCTTGGCCA TGAACTCCTC CCCCTCCGTG CGGTCCAACA TCCGGGTCAC AGTCACAGCC
    ACCGCAGTCA TCATCAACCT AGTGGTCATC ATCCTCCTGG ACGAGGTGTA TGGCTGCATA
    GCCCGATGGC TCACCAAGAT CGAGGTCCCA AAGACGGAGA AAAGCTTTGA GGAGAGGCTG
    ATCTTCAAGG CTTTCCTGCT GAAGTTTGTG AATTCCTACA CCCCCATCTT TTACGTGGCG
    TTCTTCAAAG GCCGGTTTGT TGGACGCCCG GGCGACTACG TGTACATTTT CCGTTCCTTC
    CGAATGGAAG AGTGTGCGCC AGGGGGCTGC CTGATGGAGC TATGCATCCA GCTCAGCATC
    ATCATGCTGG GGAAACAGCT GATCCAGAAC AACCTGTTCG AGATCGGCAT CCCGAAGATG
    AAGAAGCTCA TCCGCTACCT GAAGCTGAAG CAGCAGAGCC CCCCTGACCA CGAGGAGTGT
    GTGAAGAGGA AACAGCGGTA CGAGGTGGAT TACAACCTGG AGCCCTTCGC GGGCCTCACC
    CCAGAGTACA TGGAAATGAT CATCCAGTTT GGCTTCGTCA CCCTGTTTGT CGCCTCCTTC
    CCCCTGGCCC CACTGTTTGC GCTGCTGAAC AACATCATCG AGATCCGCCT GGACGCCAAA
    AAGTTTGTCA CTGAGCTCCG AAGGCCGGTA GCTGTCAGAG CCAAAGACAT CGGAATCTGG
    TACAATATCC TCAGAGGCAT TGGGAAGCTT GCTGTCATCA TCAATGCCTT CGTGATCTCC
    TTCACGTCTG ACTTCATCCC GCGCCTGGTG TACCTCTACA TGTACAGTAA GAACGGGACC
    ATGCACGGCT TCGTCAACCA CACCCTCTCC TCCTTCAACG TCAGTGACTT CCAGAACGGC
    ACGGCCCCCA ATGACCCCCT GGACCTGGGC TACGAGGTGC AGATCTGCAG GTATAAAGAC
    TACCGAGAGC CGCCGTGGTC GGAAAACAAG TACGACATCT CCAAGGACTT CTGGGCCGTC
    CTGGCAGCCC GGCTGGCGTT TGTCATCGTC TTCCAGAACC TGGTCATGTT CATGAGCGAC
    TTTGTGGACT GGGTCATCCC GGACATCCCC AAGGACATCA GCCAGCAGAT CCACAAGGAG
    AAGGTGCTCA TGGTGGAGCT GTTCATGCGG GAGGAGCAAG ACAAGCAGCA GCTGCTGGAA
    ACCTGGATGG AGAAGGAGCG GCAGAAGGAC GAGCCGCCGT GCAACCACCA CAACACCAAA
    GCCTGCCCAG ACAGCCTCGG CAGCCCAGCC CCCAGCCATG CCTACCACGG GGGCGTCCTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu68987
    Clone ID Related Accession (Same CDS sequence) XM_011545125.2
    Accession Version XM_011545125.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product anoctamin-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545125.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTATCTGTGG ACCCTGATGC CGAGTGCAAG TATGGCCTGT ACTTCAGGGA CGGCCGGCGC
    AAGGTGGACT ACATCCTGGT GTACCATCAC AAGAGGCCCT CGGGCAACCG GACCCTGGTC
    AGGAGGGTGC AGCACAGCGA CACCCCCTCT GGGGCTCGCA GCGTCAAGCA GGACCACCCC
    CTGCCGGGCA AGGGGGCGTC GCTGGATGCA GGCTCGGGGG AGCCCCCGAT GGACTACCAC
    GAGGATGACA AGCGCTTCCG CAGGGAGGAG TACGAGGGCA ACCTCCTGGA GGCGGGCCTG
    GAGCTGGAGC GGGACGAGGA CACTAAAATC CACGGAGTCG GGTTTGTGAA AATCCATGCC
    CCCTGGAACG TGCTGTGCAG AGAGGCCGAG TTTCTGAAAC TGAAGATGCC GACGAAGAAG
    ATGTACCACA TTAATGAGAC CCGTGGCCTC CTGAAAAAAA TCAACTCTGT GCTCCAGAAA
    ATCACAGATC CCATCCAGCC CAAAGTGGCT GAGCACAGGC CCCAGACCAT GAAGAGACTC
    TCCTATCCCT TCTCCCGGGA GAAGCAGCAT CTATTTGACT TGTCTGATAA GGATTCCTTT
    TTCGACAGCA AAACCCGGAG CACGATTGTC TATGAGATCT TGAAGAGAAC GACGTGTACA
    AAGGCCAAGT ACAGCATGGG GCAAGGCGAG GGAAGAAAGA AGGACTCCGC CCTTCTAAGT
    AAAAGGCGGA AATGTGGGAA GTATGGCATC ACGAGCCTGC TGGCCAATGG TGTGTACGCG
    GCTGCATACC CACTGCACGA TGGAGACTAC AACGGTGAAA ACGTCGAGTT CAACGACAGA
    AAACTCCTGT ACGAAGAGTG GGCACGCTAT GGAGTTTTCT ATAAGTACCA GCCCATCGAC
    CTGGTCAGGA AGTATTTTGG GGAGAAGATC GGCCTGTACT TCGCCTGGCT GGGCGTGTAC
    ACCCAGATGC TCATCCCTGC CTCCATCGTG GGAATCATTG TCTTCCTGTA CGGATGCGCC
    ACCATGGATG AAAACATCCC CAGCATGGAG ATGTGTGACC AGAGACACAA TATCACCATG
    TGCCCGCTTT GCGACAAGAC CTGCAGCTAC TGGAAGATGA GCTCAGCCTG CGCCACGGCC
    CGCGCCAGCC ACCTCTTCGA CAACCCCGCC ACGGTCTTCT TCTCTGTCTT CATGGCCCTC
    TGGGCTGCCA CCTTCATGGA GCACTGGAAG CGGAAACAGA TGCGACTCAA CTACCGCTGG
    GACCTCACGG GCTTTGAAGA GGAAGAGGAG GCTGTCAAGG ATCATCCTAG AGCTGAATAC
    GAAGCCAGAG TCTTGGAGAA GTCTCTGAAG AAAGAGTCCA GAAACAAAGA GACTGACAAA
    GTGAAGCTGA CATGGAGAGA TCGGTTCCCA GCCTACCTCA CTAACTTGGT CTCCATCATC
    TTCATGATTG CAGTGACGTT TGCCATCGTC CTCGGCGTCA TCATCTACAG AATCTCCATG
    GCCGCCGCCT TGGCCATGAA CTCCTCCCCC TCCGTGCGGT CCAACATCCG GGTCACAGTC
    ACAGCCACCG CAGTCATCAT CAACCTAGTG GTCATCATCC TCCTGGACGA GGTGTATGGC
    TGCATAGCCC GATGGCTCAC CAAGATCGAG GTCCCAAAGA CGGAGAAAAG CTTTGAGGAG
    AGGCTGATCT TCAAGGCTTT CCTGCTGAAG TTTGTGAATT CCTACACCCC CATCTTTTAC
    GTGGCGTTCT TCAAAGGCCG GTTTGTTGGA CGCCCGGGCG ACTACGTGTA CATTTTCCGT
    TCCTTCCGAA TGGAAGAGTG TGCGCCAGGG GGCTGCCTGA TGGAGCTATG CATCCAGCTC
    AGCATCATCA TGCTGGGGAA ACAGCTGATC CAGAACAACC TGTTCGAGAT CGGCATCCCG
    AAGATGAAGA AGCTCATCCG CTACCTGAAG CTGAAGCAGC AGAGCCCCCC TGACCACGAG
    GAGTGTGTGA AGAGGAAACA GCGGTACGAG GTGGATTACA ACCTGGAGCC CTTCGCGGGC
    CTCACCCCAG AGTACATGGA AATGATCATC CAGTTTGGCT TCGTCACCCT GTTTGTCGCC
    TCCTTCCCCC TGGCCCCACT GTTTGCGCTG CTGAACAACA TCATCGAGAT CCGCCTGGAC
    GCCAAAAAGT TTGTCACTGA GCTCCGAAGG CCGGTAGCTG TCAGAGCCAA AGACATCGGA
    ATCTGGTACA ATATCCTCAG AGGCATTGGG AAGCTTGCTG TCATCATCAA TGCCTTCGTG
    ATCTCCTTCA CGTCTGACTT CATCCCGCGC CTGGTGTACC TCTACATGTA CAGTAAGAAC
    GGGACCATGC ACGGCTTCGT CAACCACACC CTCTCCTCCT TCAACGTCAG TGACTTCCAG
    AACGGCACGG CCCCCAATGA CCCCCTGGAC CTGGGCTACG AGGTGCAGAT CTGCAGGTAT
    AAAGACTACC GAGAGCCGCC GTGGTCGGAA AACAAGTACG ACATCTCCAA GGACTTCTGG
    GCCGTCCTGG CAGCCCGGCT GGCGTTTGTC ATCGTCTTCC AGAACCTGGT CATGTTCATG
    AGCGACTTTG TGGACTGGGT CATCCCGGAC ATCCCCAAGG ACATCAGCCA GCAGATCCAC
    AAGGAGAAGG TGCTCATGGT GGAGCTGTTC ATGCGGGAGG AGCAAGACAA GCAGCAGCTG
    CTGGAAACCT GGATGGAGAA GGAGCGGCAG AAGGACGAGC CGCCGTGCAA CCACCACAAC
    ACCAAAGCCT GCCCAGACAG CCTCGGCAGC CCAGCCCCCA GCCATGCCTA CCACGGGGGC
    GTCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011543427.1
    CDS14..2962
    Translation

    Target ORF information:

    RefSeq Version XM_011545125.2
    Organism Homo sapiens(human)
    Definition Homo sapiens anoctamin 1 (ANO1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011545125.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTATCTGTGG ACCCTGATGC CGAGTGCAAG TATGGCCTGT ACTTCAGGGA CGGCCGGCGC
    AAGGTGGACT ACATCCTGGT GTACCATCAC AAGAGGCCCT CGGGCAACCG GACCCTGGTC
    AGGAGGGTGC AGCACAGCGA CACCCCCTCT GGGGCTCGCA GCGTCAAGCA GGACCACCCC
    CTGCCGGGCA AGGGGGCGTC GCTGGATGCA GGCTCGGGGG AGCCCCCGAT GGACTACCAC
    GAGGATGACA AGCGCTTCCG CAGGGAGGAG TACGAGGGCA ACCTCCTGGA GGCGGGCCTG
    GAGCTGGAGC GGGACGAGGA CACTAAAATC CACGGAGTCG GGTTTGTGAA AATCCATGCC
    CCCTGGAACG TGCTGTGCAG AGAGGCCGAG TTTCTGAAAC TGAAGATGCC GACGAAGAAG
    ATGTACCACA TTAATGAGAC CCGTGGCCTC CTGAAAAAAA TCAACTCTGT GCTCCAGAAA
    ATCACAGATC CCATCCAGCC CAAAGTGGCT GAGCACAGGC CCCAGACCAT GAAGAGACTC
    TCCTATCCCT TCTCCCGGGA GAAGCAGCAT CTATTTGACT TGTCTGATAA GGATTCCTTT
    TTCGACAGCA AAACCCGGAG CACGATTGTC TATGAGATCT TGAAGAGAAC GACGTGTACA
    AAGGCCAAGT ACAGCATGGG GCAAGGCGAG GGAAGAAAGA AGGACTCCGC CCTTCTAAGT
    AAAAGGCGGA AATGTGGGAA GTATGGCATC ACGAGCCTGC TGGCCAATGG TGTGTACGCG
    GCTGCATACC CACTGCACGA TGGAGACTAC AACGGTGAAA ACGTCGAGTT CAACGACAGA
    AAACTCCTGT ACGAAGAGTG GGCACGCTAT GGAGTTTTCT ATAAGTACCA GCCCATCGAC
    CTGGTCAGGA AGTATTTTGG GGAGAAGATC GGCCTGTACT TCGCCTGGCT GGGCGTGTAC
    ACCCAGATGC TCATCCCTGC CTCCATCGTG GGAATCATTG TCTTCCTGTA CGGATGCGCC
    ACCATGGATG AAAACATCCC CAGCATGGAG ATGTGTGACC AGAGACACAA TATCACCATG
    TGCCCGCTTT GCGACAAGAC CTGCAGCTAC TGGAAGATGA GCTCAGCCTG CGCCACGGCC
    CGCGCCAGCC ACCTCTTCGA CAACCCCGCC ACGGTCTTCT TCTCTGTCTT CATGGCCCTC
    TGGGCTGCCA CCTTCATGGA GCACTGGAAG CGGAAACAGA TGCGACTCAA CTACCGCTGG
    GACCTCACGG GCTTTGAAGA GGAAGAGGAG GCTGTCAAGG ATCATCCTAG AGCTGAATAC
    GAAGCCAGAG TCTTGGAGAA GTCTCTGAAG AAAGAGTCCA GAAACAAAGA GACTGACAAA
    GTGAAGCTGA CATGGAGAGA TCGGTTCCCA GCCTACCTCA CTAACTTGGT CTCCATCATC
    TTCATGATTG CAGTGACGTT TGCCATCGTC CTCGGCGTCA TCATCTACAG AATCTCCATG
    GCCGCCGCCT TGGCCATGAA CTCCTCCCCC TCCGTGCGGT CCAACATCCG GGTCACAGTC
    ACAGCCACCG CAGTCATCAT CAACCTAGTG GTCATCATCC TCCTGGACGA GGTGTATGGC
    TGCATAGCCC GATGGCTCAC CAAGATCGAG GTCCCAAAGA CGGAGAAAAG CTTTGAGGAG
    AGGCTGATCT TCAAGGCTTT CCTGCTGAAG TTTGTGAATT CCTACACCCC CATCTTTTAC
    GTGGCGTTCT TCAAAGGCCG GTTTGTTGGA CGCCCGGGCG ACTACGTGTA CATTTTCCGT
    TCCTTCCGAA TGGAAGAGTG TGCGCCAGGG GGCTGCCTGA TGGAGCTATG CATCCAGCTC
    AGCATCATCA TGCTGGGGAA ACAGCTGATC CAGAACAACC TGTTCGAGAT CGGCATCCCG
    AAGATGAAGA AGCTCATCCG CTACCTGAAG CTGAAGCAGC AGAGCCCCCC TGACCACGAG
    GAGTGTGTGA AGAGGAAACA GCGGTACGAG GTGGATTACA ACCTGGAGCC CTTCGCGGGC
    CTCACCCCAG AGTACATGGA AATGATCATC CAGTTTGGCT TCGTCACCCT GTTTGTCGCC
    TCCTTCCCCC TGGCCCCACT GTTTGCGCTG CTGAACAACA TCATCGAGAT CCGCCTGGAC
    GCCAAAAAGT TTGTCACTGA GCTCCGAAGG CCGGTAGCTG TCAGAGCCAA AGACATCGGA
    ATCTGGTACA ATATCCTCAG AGGCATTGGG AAGCTTGCTG TCATCATCAA TGCCTTCGTG
    ATCTCCTTCA CGTCTGACTT CATCCCGCGC CTGGTGTACC TCTACATGTA CAGTAAGAAC
    GGGACCATGC ACGGCTTCGT CAACCACACC CTCTCCTCCT TCAACGTCAG TGACTTCCAG
    AACGGCACGG CCCCCAATGA CCCCCTGGAC CTGGGCTACG AGGTGCAGAT CTGCAGGTAT
    AAAGACTACC GAGAGCCGCC GTGGTCGGAA AACAAGTACG ACATCTCCAA GGACTTCTGG
    GCCGTCCTGG CAGCCCGGCT GGCGTTTGTC ATCGTCTTCC AGAACCTGGT CATGTTCATG
    AGCGACTTTG TGGACTGGGT CATCCCGGAC ATCCCCAAGG ACATCAGCCA GCAGATCCAC
    AAGGAGAAGG TGCTCATGGT GGAGCTGTTC ATGCGGGAGG AGCAAGACAA GCAGCAGCTG
    CTGGAAACCT GGATGGAGAA GGAGCGGCAG AAGGACGAGC CGCCGTGCAA CCACCACAAC
    ACCAAAGCCT GCCCAGACAG CCTCGGCAGC CCAGCCCCCA GCCATGCCTA CCACGGGGGC
    GTCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu68988
    Clone ID Related Accession (Same CDS sequence) XM_011545126.2
    Accession Version XM_011545126.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2937bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product anoctamin-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545126.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTATCTGTGG ACCCTGATGC CGAGTGCAAG TATGGCCTGT ACTTCAGGGA CGGCCGGCGC
    AAGGTGGACT ACATCCTGGT GTACCATCAC AAGAGGCCCT CGGGCAACCG GACCCTGGTC
    AGGAGGGTGC AGCACAGCGA CACCCCCTCT GGGGCTCGCA GCGTCAAGCA GGACCACCCC
    CTGCCGGGCA AGGGGGCGTC GCTGGATGCA GGCTCGGGGG AGCCCCCGAT GGACTACCAC
    GAGGATGACA AGCGCTTCCG CAGGGAGGAG TACGAGGGCA ACCTCCTGGA GGCGGGCCTG
    GAGCTGGAGC GGGACGAGGA CACTAAAATC CACGGAGTCG GGTTTGTGAA AATCCATGCC
    CCCTGGAACG TGCTGTGCAG AGAGGCCGAG TTTCTGAAAC TGAAGATGCC GACGAAGAAG
    ATGTACCACA TTAATGAGAC CCGTGGCCTC CTGAAAAAAA TCAACTCTGT GCTCCAGAAA
    ATCACAGATC CCATCCAGCC CAAAGTGGCT GAGCACAGGC CCCAGACCAT GAAGAGACTC
    TCCTATCCCT TCTCCCGGGA GAAGCAGCAT CTATTTGACT TGTCTGATAA GGATTCCTTT
    TTCGACAGCA AAACCCGGAG CACGATTGTC TATGAGATCT TGAAGAGAAC GACGTGTACA
    AAGGCCAAGT ACAGCATGGG GCAAGGCGAG GGAAGAAAGA AGGACTCCGC CCTTCTAAGT
    AAAAGGCGGA AATGTGGGAA GTATGGCATC ACGAGCCTGC TGGCCAATGG TGTGTACGCG
    GCTGCATACC CACTGCACGA TGGAGACTAC AACGGTGAAA ACGTCGAGTT CAACGACAGA
    AAACTCCTGT ACGAAGAGTG GGCACGCTAT GGAGTTTTCT ATAAGTACCA GCCCATCGAC
    CTGGTCAGGA AGTATTTTGG GGAGAAGATC GGCCTGTACT TCGCCTGGCT GGGCGTGTAC
    ACCCAGATGC TCATCCCTGC CTCCATCGTG GGAATCATTG TCTTCCTGTA CGGATGCGCC
    ACCATGGATG AAAACATCCC CAGCATGGAG ATGTGTGACC AGAGACACAA TATCACCATG
    TGCCCGCTTT GCGACAAGAC CTGCAGCTAC TGGAAGATGA GCTCAGCCTG CGCCACGGCC
    CGCGCCAGCC ACCTCTTCGA CAACCCCGCC ACGGTCTTCT TCTCTGTCTT CATGGCCCTC
    TGGGCTGCCA CCTTCATGGA GCACTGGAAG CGGAAACAGA TGCGACTCAA CTACCGCTGG
    GACCTCACGG GCTTTGAAGA GGAAGAGGAT CATCCTAGAG CTGAATACGA AGCCAGAGTC
    TTGGAGAAGT CTCTGAAGAA AGAGTCCAGA AACAAAGAGA CTGACAAAGT GAAGCTGACA
    TGGAGAGATC GGTTCCCAGC CTACCTCACT AACTTGGTCT CCATCATCTT CATGATTGCA
    GTGACGTTTG CCATCGTCCT CGGCGTCATC ATCTACAGAA TCTCCATGGC CGCCGCCTTG
    GCCATGAACT CCTCCCCCTC CGTGCGGTCC AACATCCGGG TCACAGTCAC AGCCACCGCA
    GTCATCATCA ACCTAGTGGT CATCATCCTC CTGGACGAGG TGTATGGCTG CATAGCCCGA
    TGGCTCACCA AGATCGAGGT CCCAAAGACG GAGAAAAGCT TTGAGGAGAG GCTGATCTTC
    AAGGCTTTCC TGCTGAAGTT TGTGAATTCC TACACCCCCA TCTTTTACGT GGCGTTCTTC
    AAAGGCCGGT TTGTTGGACG CCCGGGCGAC TACGTGTACA TTTTCCGTTC CTTCCGAATG
    GAAGAGTGTG CGCCAGGGGG CTGCCTGATG GAGCTATGCA TCCAGCTCAG CATCATCATG
    CTGGGGAAAC AGCTGATCCA GAACAACCTG TTCGAGATCG GCATCCCGAA GATGAAGAAG
    CTCATCCGCT ACCTGAAGCT GAAGCAGCAG AGCCCCCCTG ACCACGAGGA GTGTGTGAAG
    AGGAAACAGC GGTACGAGGT GGATTACAAC CTGGAGCCCT TCGCGGGCCT CACCCCAGAG
    TACATGGAAA TGATCATCCA GTTTGGCTTC GTCACCCTGT TTGTCGCCTC CTTCCCCCTG
    GCCCCACTGT TTGCGCTGCT GAACAACATC ATCGAGATCC GCCTGGACGC CAAAAAGTTT
    GTCACTGAGC TCCGAAGGCC GGTAGCTGTC AGAGCCAAAG ACATCGGAAT CTGGTACAAT
    ATCCTCAGAG GCATTGGGAA GCTTGCTGTC ATCATCAATG CCTTCGTGAT CTCCTTCACG
    TCTGACTTCA TCCCGCGCCT GGTGTACCTC TACATGTACA GTAAGAACGG GACCATGCAC
    GGCTTCGTCA ACCACACCCT CTCCTCCTTC AACGTCAGTG ACTTCCAGAA CGGCACGGCC
    CCCAATGACC CCCTGGACCT GGGCTACGAG GTGCAGATCT GCAGGTATAA AGACTACCGA
    GAGCCGCCGT GGTCGGAAAA CAAGTACGAC ATCTCCAAGG ACTTCTGGGC CGTCCTGGCA
    GCCCGGCTGG CGTTTGTCAT CGTCTTCCAG AACCTGGTCA TGTTCATGAG CGACTTTGTG
    GACTGGGTCA TCCCGGACAT CCCCAAGGAC ATCAGCCAGC AGATCCACAA GGAGAAGGTG
    CTCATGGTGG AGCTGTTCAT GCGGGAGGAG CAAGACAAGC AGCAGCTGCT GGAAACCTGG
    ATGGAGAAGG AGCGGCAGAA GGACGAGCCG CCGTGCAACC ACCACAACAC CAAAGCCTGC
    CCAGACAGCC TCGGCAGCCC AGCCCCCAGC CATGCCTACC ACGGGGGCGT CCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011543428.1
    CDS14..2950
    Translation

    Target ORF information:

    RefSeq Version XM_011545126.2
    Organism Homo sapiens(human)
    Definition Homo sapiens anoctamin 1 (ANO1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011545126.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTATCTGTGG ACCCTGATGC CGAGTGCAAG TATGGCCTGT ACTTCAGGGA CGGCCGGCGC
    AAGGTGGACT ACATCCTGGT GTACCATCAC AAGAGGCCCT CGGGCAACCG GACCCTGGTC
    AGGAGGGTGC AGCACAGCGA CACCCCCTCT GGGGCTCGCA GCGTCAAGCA GGACCACCCC
    CTGCCGGGCA AGGGGGCGTC GCTGGATGCA GGCTCGGGGG AGCCCCCGAT GGACTACCAC
    GAGGATGACA AGCGCTTCCG CAGGGAGGAG TACGAGGGCA ACCTCCTGGA GGCGGGCCTG
    GAGCTGGAGC GGGACGAGGA CACTAAAATC CACGGAGTCG GGTTTGTGAA AATCCATGCC
    CCCTGGAACG TGCTGTGCAG AGAGGCCGAG TTTCTGAAAC TGAAGATGCC GACGAAGAAG
    ATGTACCACA TTAATGAGAC CCGTGGCCTC CTGAAAAAAA TCAACTCTGT GCTCCAGAAA
    ATCACAGATC CCATCCAGCC CAAAGTGGCT GAGCACAGGC CCCAGACCAT GAAGAGACTC
    TCCTATCCCT TCTCCCGGGA GAAGCAGCAT CTATTTGACT TGTCTGATAA GGATTCCTTT
    TTCGACAGCA AAACCCGGAG CACGATTGTC TATGAGATCT TGAAGAGAAC GACGTGTACA
    AAGGCCAAGT ACAGCATGGG GCAAGGCGAG GGAAGAAAGA AGGACTCCGC CCTTCTAAGT
    AAAAGGCGGA AATGTGGGAA GTATGGCATC ACGAGCCTGC TGGCCAATGG TGTGTACGCG
    GCTGCATACC CACTGCACGA TGGAGACTAC AACGGTGAAA ACGTCGAGTT CAACGACAGA
    AAACTCCTGT ACGAAGAGTG GGCACGCTAT GGAGTTTTCT ATAAGTACCA GCCCATCGAC
    CTGGTCAGGA AGTATTTTGG GGAGAAGATC GGCCTGTACT TCGCCTGGCT GGGCGTGTAC
    ACCCAGATGC TCATCCCTGC CTCCATCGTG GGAATCATTG TCTTCCTGTA CGGATGCGCC
    ACCATGGATG AAAACATCCC CAGCATGGAG ATGTGTGACC AGAGACACAA TATCACCATG
    TGCCCGCTTT GCGACAAGAC CTGCAGCTAC TGGAAGATGA GCTCAGCCTG CGCCACGGCC
    CGCGCCAGCC ACCTCTTCGA CAACCCCGCC ACGGTCTTCT TCTCTGTCTT CATGGCCCTC
    TGGGCTGCCA CCTTCATGGA GCACTGGAAG CGGAAACAGA TGCGACTCAA CTACCGCTGG
    GACCTCACGG GCTTTGAAGA GGAAGAGGAT CATCCTAGAG CTGAATACGA AGCCAGAGTC
    TTGGAGAAGT CTCTGAAGAA AGAGTCCAGA AACAAAGAGA CTGACAAAGT GAAGCTGACA
    TGGAGAGATC GGTTCCCAGC CTACCTCACT AACTTGGTCT CCATCATCTT CATGATTGCA
    GTGACGTTTG CCATCGTCCT CGGCGTCATC ATCTACAGAA TCTCCATGGC CGCCGCCTTG
    GCCATGAACT CCTCCCCCTC CGTGCGGTCC AACATCCGGG TCACAGTCAC AGCCACCGCA
    GTCATCATCA ACCTAGTGGT CATCATCCTC CTGGACGAGG TGTATGGCTG CATAGCCCGA
    TGGCTCACCA AGATCGAGGT CCCAAAGACG GAGAAAAGCT TTGAGGAGAG GCTGATCTTC
    AAGGCTTTCC TGCTGAAGTT TGTGAATTCC TACACCCCCA TCTTTTACGT GGCGTTCTTC
    AAAGGCCGGT TTGTTGGACG CCCGGGCGAC TACGTGTACA TTTTCCGTTC CTTCCGAATG
    GAAGAGTGTG CGCCAGGGGG CTGCCTGATG GAGCTATGCA TCCAGCTCAG CATCATCATG
    CTGGGGAAAC AGCTGATCCA GAACAACCTG TTCGAGATCG GCATCCCGAA GATGAAGAAG
    CTCATCCGCT ACCTGAAGCT GAAGCAGCAG AGCCCCCCTG ACCACGAGGA GTGTGTGAAG
    AGGAAACAGC GGTACGAGGT GGATTACAAC CTGGAGCCCT TCGCGGGCCT CACCCCAGAG
    TACATGGAAA TGATCATCCA GTTTGGCTTC GTCACCCTGT TTGTCGCCTC CTTCCCCCTG
    GCCCCACTGT TTGCGCTGCT GAACAACATC ATCGAGATCC GCCTGGACGC CAAAAAGTTT
    GTCACTGAGC TCCGAAGGCC GGTAGCTGTC AGAGCCAAAG ACATCGGAAT CTGGTACAAT
    ATCCTCAGAG GCATTGGGAA GCTTGCTGTC ATCATCAATG CCTTCGTGAT CTCCTTCACG
    TCTGACTTCA TCCCGCGCCT GGTGTACCTC TACATGTACA GTAAGAACGG GACCATGCAC
    GGCTTCGTCA ACCACACCCT CTCCTCCTTC AACGTCAGTG ACTTCCAGAA CGGCACGGCC
    CCCAATGACC CCCTGGACCT GGGCTACGAG GTGCAGATCT GCAGGTATAA AGACTACCGA
    GAGCCGCCGT GGTCGGAAAA CAAGTACGAC ATCTCCAAGG ACTTCTGGGC CGTCCTGGCA
    GCCCGGCTGG CGTTTGTCAT CGTCTTCCAG AACCTGGTCA TGTTCATGAG CGACTTTGTG
    GACTGGGTCA TCCCGGACAT CCCCAAGGAC ATCAGCCAGC AGATCCACAA GGAGAAGGTG
    CTCATGGTGG AGCTGTTCAT GCGGGAGGAG CAAGACAAGC AGCAGCTGCT GGAAACCTGG
    ATGGAGAAGG AGCGGCAGAA GGACGAGCCG CCGTGCAACC ACCACAACAC CAAAGCCTGC
    CCAGACAGCC TCGGCAGCCC AGCCCCCAGC CATGCCTACC ACGGGGGCGT CCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu68989
    Clone ID Related Accession (Same CDS sequence) XM_011545127.2
    Accession Version XM_011545127.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product anoctamin-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545127.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTATCTGTGG ACCCTGATGC CGAGTGCAAG TATGGCCTGT ACTTCAGGGA CGGCCGGCGC
    AAGGTGGACT ACATCCTGGT GTACCATCAC AAGAGGCCCT CGGGCAACCG GACCCTGGTC
    AGGAGGGTGC AGCACAGCGA CACCCCCTCT GGGGCTCGCA GCGTCAAGCA GGACCACCCC
    CTGCCGGGCA AGGGGGCGTC GCTGGATGCA GGCTCGGGGG AGCCCCCGAT GGACTACCAC
    GAGGATGACA AGCGCTTCCG CAGGGAGGAG TACGAGGGCA ACCTCCTGGA GGCGGGCCTG
    GAGCTGGAGC GGGACGAGGA CACTAAAATC CACGGAGTCG GGTTTGTGAA AATCCATGCC
    CCCTGGAACG TGCTGTGCAG AGAGGCCGAG TTTCTGAAAC TGAAGATGCC GACGAAGAAG
    ATGTACCACA TTAATGAGAC CCGTGGCCTC CTGAAAAAAA TCAACTCTGT GCTCCAGAAA
    ATCACAGATC CCATCCAGCC CAAAGTGGCT GAGCACAGGC CCCAGACCAT GAAGAGACTC
    TCCTATCCCT TCTCCCGGGA GAAGCAGCAT CTATTTGACT TGTCTGATAA GGATTCCTTT
    TTCGACAGCA AAACCCGGAG CACGATTGTC TATGAGATCT TGAAGAGAAC GACGTGTACA
    AAGGCCAAGT ACAGCATGGG CATCACGAGC CTGCTGGCCA ATGGTGTGTA CGCGGCTGCA
    TACCCACTGC ACGATGGAGA CTACAACGGT GAAAACGTCG AGTTCAACGA CAGAAAACTC
    CTGTACGAAG AGTGGGCACG CTATGGAGTT TTCTATAAGT ACCAGCCCAT CGACCTGGTC
    AGGAAGTATT TTGGGGAGAA GATCGGCCTG TACTTCGCCT GGCTGGGCGT GTACACCCAG
    ATGCTCATCC CTGCCTCCAT CGTGGGAATC ATTGTCTTCC TGTACGGATG CGCCACCATG
    GATGAAAACA TCCCCAGCAT GGAGATGTGT GACCAGAGAC ACAATATCAC CATGTGCCCG
    CTTTGCGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGCGCCAC GGCCCGCGCC
    AGCCACCTCT TCGACAACCC CGCCACGGTC TTCTTCTCTG TCTTCATGGC CCTCTGGGCT
    GCCACCTTCA TGGAGCACTG GAAGCGGAAA CAGATGCGAC TCAACTACCG CTGGGACCTC
    ACGGGCTTTG AAGAGGAAGA GGAGGCTGTC AAGGATCATC CTAGAGCTGA ATACGAAGCC
    AGAGTCTTGG AGAAGTCTCT GAAGAAAGAG TCCAGAAACA AAGAGACTGA CAAAGTGAAG
    CTGACATGGA GAGATCGGTT CCCAGCCTAC CTCACTAACT TGGTCTCCAT CATCTTCATG
    ATTGCAGTGA CGTTTGCCAT CGTCCTCGGC GTCATCATCT ACAGAATCTC CATGGCCGCC
    GCCTTGGCCA TGAACTCCTC CCCCTCCGTG CGGTCCAACA TCCGGGTCAC AGTCACAGCC
    ACCGCAGTCA TCATCAACCT AGTGGTCATC ATCCTCCTGG ACGAGGTGTA TGGCTGCATA
    GCCCGATGGC TCACCAAGAT CGAGGTCCCA AAGACGGAGA AAAGCTTTGA GGAGAGGCTG
    ATCTTCAAGG CTTTCCTGCT GAAGTTTGTG AATTCCTACA CCCCCATCTT TTACGTGGCG
    TTCTTCAAAG GCCGGTTTGT TGGACGCCCG GGCGACTACG TGTACATTTT CCGTTCCTTC
    CGAATGGAAG AGTGTGCGCC AGGGGGCTGC CTGATGGAGC TATGCATCCA GCTCAGCATC
    ATCATGCTGG GGAAACAGCT GATCCAGAAC AACCTGTTCG AGATCGGCAT CCCGAAGATG
    AAGAAGCTCA TCCGCTACCT GAAGCTGAAG CAGCAGAGCC CCCCTGACCA CGAGGAGTGT
    GTGAAGAGGA AACAGCGGTA CGAGGTGGAT TACAACCTGG AGCCCTTCGC GGGCCTCACC
    CCAGAGTACA TGGAAATGAT CATCCAGTTT GGCTTCGTCA CCCTGTTTGT CGCCTCCTTC
    CCCCTGGCCC CACTGTTTGC GCTGCTGAAC AACATCATCG AGATCCGCCT GGACGCCAAA
    AAGTTTGTCA CTGAGCTCCG AAGGCCGGTA GCTGTCAGAG CCAAAGACAT CGGAATCTGG
    TACAATATCC TCAGAGGCAT TGGGAAGCTT GCTGTCATCA TCAATGCCTT CGTGATCTCC
    TTCACGTCTG ACTTCATCCC GCGCCTGGTG TACCTCTACA TGTACAGTAA GAACGGGACC
    ATGCACGGCT TCGTCAACCA CACCCTCTCC TCCTTCAACG TCAGTGACTT CCAGAACGGC
    ACGGCCCCCA ATGACCCCCT GGACCTGGGC TACGAGGTGC AGATCTGCAG GTATAAAGAC
    TACCGAGAGC CGCCGTGGTC GGAAAACAAG TACGACATCT CCAAGGACTT CTGGGCCGTC
    CTGGCAGCCC GGCTGGCGTT TGTCATCGTC TTCCAGAACC TGGTCATGTT CATGAGCGAC
    TTTGTGGACT GGGTCATCCC GGACATCCCC AAGGACATCA GCCAGCAGAT CCACAAGGAG
    AAGGTGCTCA TGGTGGAGCT GTTCATGCGG GAGGAGCAAG ACAAGCAGCA GCTGCTGGAA
    ACCTGGATGG AGAAGGAGCG GCAGAAGGAC GAGCCGCCGT GCAACCACCA CAACACCAAA
    GCCTGCCCAG ACAGCCTCGG CAGCCCAGCC CCCAGCCATG CCTACCACGG GGGCGTCCTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011543429.1
    CDS14..2896
    Translation

    Target ORF information:

    RefSeq Version XM_011545127.2
    Organism Homo sapiens(human)
    Definition Homo sapiens anoctamin 1 (ANO1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011545127.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTATCTGTGG ACCCTGATGC CGAGTGCAAG TATGGCCTGT ACTTCAGGGA CGGCCGGCGC
    AAGGTGGACT ACATCCTGGT GTACCATCAC AAGAGGCCCT CGGGCAACCG GACCCTGGTC
    AGGAGGGTGC AGCACAGCGA CACCCCCTCT GGGGCTCGCA GCGTCAAGCA GGACCACCCC
    CTGCCGGGCA AGGGGGCGTC GCTGGATGCA GGCTCGGGGG AGCCCCCGAT GGACTACCAC
    GAGGATGACA AGCGCTTCCG CAGGGAGGAG TACGAGGGCA ACCTCCTGGA GGCGGGCCTG
    GAGCTGGAGC GGGACGAGGA CACTAAAATC CACGGAGTCG GGTTTGTGAA AATCCATGCC
    CCCTGGAACG TGCTGTGCAG AGAGGCCGAG TTTCTGAAAC TGAAGATGCC GACGAAGAAG
    ATGTACCACA TTAATGAGAC CCGTGGCCTC CTGAAAAAAA TCAACTCTGT GCTCCAGAAA
    ATCACAGATC CCATCCAGCC CAAAGTGGCT GAGCACAGGC CCCAGACCAT GAAGAGACTC
    TCCTATCCCT TCTCCCGGGA GAAGCAGCAT CTATTTGACT TGTCTGATAA GGATTCCTTT
    TTCGACAGCA AAACCCGGAG CACGATTGTC TATGAGATCT TGAAGAGAAC GACGTGTACA
    AAGGCCAAGT ACAGCATGGG CATCACGAGC CTGCTGGCCA ATGGTGTGTA CGCGGCTGCA
    TACCCACTGC ACGATGGAGA CTACAACGGT GAAAACGTCG AGTTCAACGA CAGAAAACTC
    CTGTACGAAG AGTGGGCACG CTATGGAGTT TTCTATAAGT ACCAGCCCAT CGACCTGGTC
    AGGAAGTATT TTGGGGAGAA GATCGGCCTG TACTTCGCCT GGCTGGGCGT GTACACCCAG
    ATGCTCATCC CTGCCTCCAT CGTGGGAATC ATTGTCTTCC TGTACGGATG CGCCACCATG
    GATGAAAACA TCCCCAGCAT GGAGATGTGT GACCAGAGAC ACAATATCAC CATGTGCCCG
    CTTTGCGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGCGCCAC GGCCCGCGCC
    AGCCACCTCT TCGACAACCC CGCCACGGTC TTCTTCTCTG TCTTCATGGC CCTCTGGGCT
    GCCACCTTCA TGGAGCACTG GAAGCGGAAA CAGATGCGAC TCAACTACCG CTGGGACCTC
    ACGGGCTTTG AAGAGGAAGA GGAGGCTGTC AAGGATCATC CTAGAGCTGA ATACGAAGCC
    AGAGTCTTGG AGAAGTCTCT GAAGAAAGAG TCCAGAAACA AAGAGACTGA CAAAGTGAAG
    CTGACATGGA GAGATCGGTT CCCAGCCTAC CTCACTAACT TGGTCTCCAT CATCTTCATG
    ATTGCAGTGA CGTTTGCCAT CGTCCTCGGC GTCATCATCT ACAGAATCTC CATGGCCGCC
    GCCTTGGCCA TGAACTCCTC CCCCTCCGTG CGGTCCAACA TCCGGGTCAC AGTCACAGCC
    ACCGCAGTCA TCATCAACCT AGTGGTCATC ATCCTCCTGG ACGAGGTGTA TGGCTGCATA
    GCCCGATGGC TCACCAAGAT CGAGGTCCCA AAGACGGAGA AAAGCTTTGA GGAGAGGCTG
    ATCTTCAAGG CTTTCCTGCT GAAGTTTGTG AATTCCTACA CCCCCATCTT TTACGTGGCG
    TTCTTCAAAG GCCGGTTTGT TGGACGCCCG GGCGACTACG TGTACATTTT CCGTTCCTTC
    CGAATGGAAG AGTGTGCGCC AGGGGGCTGC CTGATGGAGC TATGCATCCA GCTCAGCATC
    ATCATGCTGG GGAAACAGCT GATCCAGAAC AACCTGTTCG AGATCGGCAT CCCGAAGATG
    AAGAAGCTCA TCCGCTACCT GAAGCTGAAG CAGCAGAGCC CCCCTGACCA CGAGGAGTGT
    GTGAAGAGGA AACAGCGGTA CGAGGTGGAT TACAACCTGG AGCCCTTCGC GGGCCTCACC
    CCAGAGTACA TGGAAATGAT CATCCAGTTT GGCTTCGTCA CCCTGTTTGT CGCCTCCTTC
    CCCCTGGCCC CACTGTTTGC GCTGCTGAAC AACATCATCG AGATCCGCCT GGACGCCAAA
    AAGTTTGTCA CTGAGCTCCG AAGGCCGGTA GCTGTCAGAG CCAAAGACAT CGGAATCTGG
    TACAATATCC TCAGAGGCAT TGGGAAGCTT GCTGTCATCA TCAATGCCTT CGTGATCTCC
    TTCACGTCTG ACTTCATCCC GCGCCTGGTG TACCTCTACA TGTACAGTAA GAACGGGACC
    ATGCACGGCT TCGTCAACCA CACCCTCTCC TCCTTCAACG TCAGTGACTT CCAGAACGGC
    ACGGCCCCCA ATGACCCCCT GGACCTGGGC TACGAGGTGC AGATCTGCAG GTATAAAGAC
    TACCGAGAGC CGCCGTGGTC GGAAAACAAG TACGACATCT CCAAGGACTT CTGGGCCGTC
    CTGGCAGCCC GGCTGGCGTT TGTCATCGTC TTCCAGAACC TGGTCATGTT CATGAGCGAC
    TTTGTGGACT GGGTCATCCC GGACATCCCC AAGGACATCA GCCAGCAGAT CCACAAGGAG
    AAGGTGCTCA TGGTGGAGCT GTTCATGCGG GAGGAGCAAG ACAAGCAGCA GCTGCTGGAA
    ACCTGGATGG AGAAGGAGCG GCAGAAGGAC GAGCCGCCGT GCAACCACCA CAACACCAAA
    GCCTGCCCAG ACAGCCTCGG CAGCCCAGCC CCCAGCCATG CCTACCACGG GGGCGTCCTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu68990
    Clone ID Related Accession (Same CDS sequence) XM_011545128.2
    Accession Version XM_011545128.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2871bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product anoctamin-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545128.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTATCTGTGG ACCCTGATGC CGAGTGCAAG TATGGCCTGT ACTTCAGGGA CGGCCGGCGC
    AAGGTGGACT ACATCCTGGT GTACCATCAC AAGAGGCCCT CGGGCAACCG GACCCTGGTC
    AGGAGGGTGC AGCACAGCGA CACCCCCTCT GGGGCTCGCA GCGTCAAGCA GGACCACCCC
    CTGCCGGGCA AGGGGGCGTC GCTGGATGCA GGCTCGGGGG AGCCCCCGAT GGACTACCAC
    GAGGATGACA AGCGCTTCCG CAGGGAGGAG TACGAGGGCA ACCTCCTGGA GGCGGGCCTG
    GAGCTGGAGC GGGACGAGGA CACTAAAATC CACGGAGTCG GGTTTGTGAA AATCCATGCC
    CCCTGGAACG TGCTGTGCAG AGAGGCCGAG TTTCTGAAAC TGAAGATGCC GACGAAGAAG
    ATGTACCACA TTAATGAGAC CCGTGGCCTC CTGAAAAAAA TCAACTCTGT GCTCCAGAAA
    ATCACAGATC CCATCCAGCC CAAAGTGGCT GAGCACAGGC CCCAGACCAT GAAGAGACTC
    TCCTATCCCT TCTCCCGGGA GAAGCAGCAT CTATTTGACT TGTCTGATAA GGATTCCTTT
    TTCGACAGCA AAACCCGGAG CACGATTGTC TATGAGATCT TGAAGAGAAC GACGTGTACA
    AAGGCCAAGT ACAGCATGGG CATCACGAGC CTGCTGGCCA ATGGTGTGTA CGCGGCTGCA
    TACCCACTGC ACGATGGAGA CTACAACGGT GAAAACGTCG AGTTCAACGA CAGAAAACTC
    CTGTACGAAG AGTGGGCACG CTATGGAGTT TTCTATAAGT ACCAGCCCAT CGACCTGGTC
    AGGAAGTATT TTGGGGAGAA GATCGGCCTG TACTTCGCCT GGCTGGGCGT GTACACCCAG
    ATGCTCATCC CTGCCTCCAT CGTGGGAATC ATTGTCTTCC TGTACGGATG CGCCACCATG
    GATGAAAACA TCCCCAGCAT GGAGATGTGT GACCAGAGAC ACAATATCAC CATGTGCCCG
    CTTTGCGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGCGCCAC GGCCCGCGCC
    AGCCACCTCT TCGACAACCC CGCCACGGTC TTCTTCTCTG TCTTCATGGC CCTCTGGGCT
    GCCACCTTCA TGGAGCACTG GAAGCGGAAA CAGATGCGAC TCAACTACCG CTGGGACCTC
    ACGGGCTTTG AAGAGGAAGA GGATCATCCT AGAGCTGAAT ACGAAGCCAG AGTCTTGGAG
    AAGTCTCTGA AGAAAGAGTC CAGAAACAAA GAGACTGACA AAGTGAAGCT GACATGGAGA
    GATCGGTTCC CAGCCTACCT CACTAACTTG GTCTCCATCA TCTTCATGAT TGCAGTGACG
    TTTGCCATCG TCCTCGGCGT CATCATCTAC AGAATCTCCA TGGCCGCCGC CTTGGCCATG
    AACTCCTCCC CCTCCGTGCG GTCCAACATC CGGGTCACAG TCACAGCCAC CGCAGTCATC
    ATCAACCTAG TGGTCATCAT CCTCCTGGAC GAGGTGTATG GCTGCATAGC CCGATGGCTC
    ACCAAGATCG AGGTCCCAAA GACGGAGAAA AGCTTTGAGG AGAGGCTGAT CTTCAAGGCT
    TTCCTGCTGA AGTTTGTGAA TTCCTACACC CCCATCTTTT ACGTGGCGTT CTTCAAAGGC
    CGGTTTGTTG GACGCCCGGG CGACTACGTG TACATTTTCC GTTCCTTCCG AATGGAAGAG
    TGTGCGCCAG GGGGCTGCCT GATGGAGCTA TGCATCCAGC TCAGCATCAT CATGCTGGGG
    AAACAGCTGA TCCAGAACAA CCTGTTCGAG ATCGGCATCC CGAAGATGAA GAAGCTCATC
    CGCTACCTGA AGCTGAAGCA GCAGAGCCCC CCTGACCACG AGGAGTGTGT GAAGAGGAAA
    CAGCGGTACG AGGTGGATTA CAACCTGGAG CCCTTCGCGG GCCTCACCCC AGAGTACATG
    GAAATGATCA TCCAGTTTGG CTTCGTCACC CTGTTTGTCG CCTCCTTCCC CCTGGCCCCA
    CTGTTTGCGC TGCTGAACAA CATCATCGAG ATCCGCCTGG ACGCCAAAAA GTTTGTCACT
    GAGCTCCGAA GGCCGGTAGC TGTCAGAGCC AAAGACATCG GAATCTGGTA CAATATCCTC
    AGAGGCATTG GGAAGCTTGC TGTCATCATC AATGCCTTCG TGATCTCCTT CACGTCTGAC
    TTCATCCCGC GCCTGGTGTA CCTCTACATG TACAGTAAGA ACGGGACCAT GCACGGCTTC
    GTCAACCACA CCCTCTCCTC CTTCAACGTC AGTGACTTCC AGAACGGCAC GGCCCCCAAT
    GACCCCCTGG ACCTGGGCTA CGAGGTGCAG ATCTGCAGGT ATAAAGACTA CCGAGAGCCG
    CCGTGGTCGG AAAACAAGTA CGACATCTCC AAGGACTTCT GGGCCGTCCT GGCAGCCCGG
    CTGGCGTTTG TCATCGTCTT CCAGAACCTG GTCATGTTCA TGAGCGACTT TGTGGACTGG
    GTCATCCCGG ACATCCCCAA GGACATCAGC CAGCAGATCC ACAAGGAGAA GGTGCTCATG
    GTGGAGCTGT TCATGCGGGA GGAGCAAGAC AAGCAGCAGC TGCTGGAAAC CTGGATGGAG
    AAGGAGCGGC AGAAGGACGA GCCGCCGTGC AACCACCACA ACACCAAAGC CTGCCCAGAC
    AGCCTCGGCA GCCCAGCCCC CAGCCATGCC TACCACGGGG GCGTCCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011543430.1
    CDS14..2884
    Translation

    Target ORF information:

    RefSeq Version XM_011545128.2
    Organism Homo sapiens(human)
    Definition Homo sapiens anoctamin 1 (ANO1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011545128.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTATCTGTGG ACCCTGATGC CGAGTGCAAG TATGGCCTGT ACTTCAGGGA CGGCCGGCGC
    AAGGTGGACT ACATCCTGGT GTACCATCAC AAGAGGCCCT CGGGCAACCG GACCCTGGTC
    AGGAGGGTGC AGCACAGCGA CACCCCCTCT GGGGCTCGCA GCGTCAAGCA GGACCACCCC
    CTGCCGGGCA AGGGGGCGTC GCTGGATGCA GGCTCGGGGG AGCCCCCGAT GGACTACCAC
    GAGGATGACA AGCGCTTCCG CAGGGAGGAG TACGAGGGCA ACCTCCTGGA GGCGGGCCTG
    GAGCTGGAGC GGGACGAGGA CACTAAAATC CACGGAGTCG GGTTTGTGAA AATCCATGCC
    CCCTGGAACG TGCTGTGCAG AGAGGCCGAG TTTCTGAAAC TGAAGATGCC GACGAAGAAG
    ATGTACCACA TTAATGAGAC CCGTGGCCTC CTGAAAAAAA TCAACTCTGT GCTCCAGAAA
    ATCACAGATC CCATCCAGCC CAAAGTGGCT GAGCACAGGC CCCAGACCAT GAAGAGACTC
    TCCTATCCCT TCTCCCGGGA GAAGCAGCAT CTATTTGACT TGTCTGATAA GGATTCCTTT
    TTCGACAGCA AAACCCGGAG CACGATTGTC TATGAGATCT TGAAGAGAAC GACGTGTACA
    AAGGCCAAGT ACAGCATGGG CATCACGAGC CTGCTGGCCA ATGGTGTGTA CGCGGCTGCA
    TACCCACTGC ACGATGGAGA CTACAACGGT GAAAACGTCG AGTTCAACGA CAGAAAACTC
    CTGTACGAAG AGTGGGCACG CTATGGAGTT TTCTATAAGT ACCAGCCCAT CGACCTGGTC
    AGGAAGTATT TTGGGGAGAA GATCGGCCTG TACTTCGCCT GGCTGGGCGT GTACACCCAG
    ATGCTCATCC CTGCCTCCAT CGTGGGAATC ATTGTCTTCC TGTACGGATG CGCCACCATG
    GATGAAAACA TCCCCAGCAT GGAGATGTGT GACCAGAGAC ACAATATCAC CATGTGCCCG
    CTTTGCGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGCGCCAC GGCCCGCGCC
    AGCCACCTCT TCGACAACCC CGCCACGGTC TTCTTCTCTG TCTTCATGGC CCTCTGGGCT
    GCCACCTTCA TGGAGCACTG GAAGCGGAAA CAGATGCGAC TCAACTACCG CTGGGACCTC
    ACGGGCTTTG AAGAGGAAGA GGATCATCCT AGAGCTGAAT ACGAAGCCAG AGTCTTGGAG
    AAGTCTCTGA AGAAAGAGTC CAGAAACAAA GAGACTGACA AAGTGAAGCT GACATGGAGA
    GATCGGTTCC CAGCCTACCT CACTAACTTG GTCTCCATCA TCTTCATGAT TGCAGTGACG
    TTTGCCATCG TCCTCGGCGT CATCATCTAC AGAATCTCCA TGGCCGCCGC CTTGGCCATG
    AACTCCTCCC CCTCCGTGCG GTCCAACATC CGGGTCACAG TCACAGCCAC CGCAGTCATC
    ATCAACCTAG TGGTCATCAT CCTCCTGGAC GAGGTGTATG GCTGCATAGC CCGATGGCTC
    ACCAAGATCG AGGTCCCAAA GACGGAGAAA AGCTTTGAGG AGAGGCTGAT CTTCAAGGCT
    TTCCTGCTGA AGTTTGTGAA TTCCTACACC CCCATCTTTT ACGTGGCGTT CTTCAAAGGC
    CGGTTTGTTG GACGCCCGGG CGACTACGTG TACATTTTCC GTTCCTTCCG AATGGAAGAG
    TGTGCGCCAG GGGGCTGCCT GATGGAGCTA TGCATCCAGC TCAGCATCAT CATGCTGGGG
    AAACAGCTGA TCCAGAACAA CCTGTTCGAG ATCGGCATCC CGAAGATGAA GAAGCTCATC
    CGCTACCTGA AGCTGAAGCA GCAGAGCCCC CCTGACCACG AGGAGTGTGT GAAGAGGAAA
    CAGCGGTACG AGGTGGATTA CAACCTGGAG CCCTTCGCGG GCCTCACCCC AGAGTACATG
    GAAATGATCA TCCAGTTTGG CTTCGTCACC CTGTTTGTCG CCTCCTTCCC CCTGGCCCCA
    CTGTTTGCGC TGCTGAACAA CATCATCGAG ATCCGCCTGG ACGCCAAAAA GTTTGTCACT
    GAGCTCCGAA GGCCGGTAGC TGTCAGAGCC AAAGACATCG GAATCTGGTA CAATATCCTC
    AGAGGCATTG GGAAGCTTGC TGTCATCATC AATGCCTTCG TGATCTCCTT CACGTCTGAC
    TTCATCCCGC GCCTGGTGTA CCTCTACATG TACAGTAAGA ACGGGACCAT GCACGGCTTC
    GTCAACCACA CCCTCTCCTC CTTCAACGTC AGTGACTTCC AGAACGGCAC GGCCCCCAAT
    GACCCCCTGG ACCTGGGCTA CGAGGTGCAG ATCTGCAGGT ATAAAGACTA CCGAGAGCCG
    CCGTGGTCGG AAAACAAGTA CGACATCTCC AAGGACTTCT GGGCCGTCCT GGCAGCCCGG
    CTGGCGTTTG TCATCGTCTT CCAGAACCTG GTCATGTTCA TGAGCGACTT TGTGGACTGG
    GTCATCCCGG ACATCCCCAA GGACATCAGC CAGCAGATCC ACAAGGAGAA GGTGCTCATG
    GTGGAGCTGT TCATGCGGGA GGAGCAAGAC AAGCAGCAGC TGCTGGAAAC CTGGATGGAG
    AAGGAGCGGC AGAAGGACGA GCCGCCGTGC AACCACCACA ACACCAAAGC CTGCCCAGAC
    AGCCTCGGCA GCCCAGCCCC CAGCCATGCC TACCACGGGG GCGTCCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu68991
    Clone ID Related Accession (Same CDS sequence) XM_011545129.2
    Accession Version XM_011545129.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2940bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product anoctamin-1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545129.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCTGACCA GGCCCTCCCA GCTGCTGAAC TCCTTATCTG TGGACCCTGA TGCCGAGTGC 
    AAGTATGGCC TGTACTTCAG GGACGGCCGG CGCAAGGTGG ACTACATCCT GGTGTACCAT
    CACAAGAGGC CCTCGGGCAA CCGGACCCTG GTCAGGAGGG TGCAGCACAG CGACACCCCC
    TCTGGGGCTC GCAGCGTCAA GCAGGACCAC CCCCTGCCGG GCAAGGGGGC GTCGCTGGAT
    GCAGGCTCGG GGGAGCCCCC GATGGACTAC CACGAGGATG ACAAGCGCTT CCGCAGGGAG
    GAGTACGAGG GCAACCTCCT GGAGGCGGGC CTGGAGCTGG AGCGGGACGA GGACACTAAA
    ATCCACGGAG TCGGGTTTGT GAAAATCCAT GCCCCCTGGA ACGTGCTGTG CAGAGAGGCC
    GAGTTTCTGA AACTGAAGAT GCCGACGAAG AAGATGTACC ACATTAATGA GACCCGTGGC
    CTCCTGAAAA AAATCAACTC TGTGCTCCAG AAAATCACAG ATCCCATCCA GCCCAAAGTG
    GCTGAGCACA GGCCCCAGAC CATGAAGAGA CTCTCCTATC CCTTCTCCCG GGAGAAGCAG
    CATCTATTTG ACTTGTCTGA TAAGGATTCC TTTTTCGACA GCAAAACCCG GAGCACGATT
    GTCTATGAGA TCTTGAAGAG AACGACGTGT ACAAAGGCCA AGTACAGCAT GGGGCAAGGC
    GAGGGAAGAA AGAAGGACTC CGCCCTTCTA AGTAAAAGGC GGAAATGTGG GAAGTATGGC
    ATCACGAGCC TGCTGGCCAA TGGTGTGTAC GCGGCTGCAT ACCCACTGCA CGATGGAGAC
    TACAACGGTG AAAACGTCGA GTTCAACGAC AGAAAACTCC TGTACGAAGA GTGGGCACGC
    TATGGAGTTT TCTATAAGTA CCAGCCCATC GACCTGGTCA GGAAGTATTT TGGGGAGAAG
    ATCGGCCTGT ACTTCGCCTG GCTGGGCGTG TACACCCAGA TGCTCATCCC TGCCTCCATC
    GTGGGAATCA TTGTCTTCCT GTACGGATGC GCCACCATGG ATGAAAACAT CCCCAGCATG
    GAGATGTGTG ACCAGAGACA CAATATCACC ATGTGCCCGC TTTGCGACAA GACCTGCAGC
    TACTGGAAGA TGAGCTCAGC CTGCGCCACG GCCCGCGCCA GCCACCTCTT CGACAACCCC
    GCCACGGTCT TCTTCTCTGT CTTCATGGCC CTCTGGGCTG CCACCTTCAT GGAGCACTGG
    AAGCGGAAAC AGATGCGACT CAACTACCGC TGGGACCTCA CGGGCTTTGA AGAGGAAGAG
    GAGGCTGTCA AGGATCATCC TAGAGCTGAA TACGAAGCCA GAGTCTTGGA GAAGTCTCTG
    AAGAAAGAGT CCAGAAACAA AGAGAAGCGC CGGCATATTC CAGAGGAGTC AACAAACAAA
    TGGAAGCAGA GGGTTAAGAC AGCCATGGCG GGGGTGAAAT TGACTGACAA AGTGAAGCTG
    ACATGGAGAG ATCGGTTCCC AGCCTACCTC ACTAACTTGG TCTCCATCAT CTTCATGATT
    GCAGTGACGT TTGCCATCGT CCTCGGCGTC ATCATCTACA GAATCTCCAT GGCCGCCGCC
    TTGGCCATGA ACTCCTCCCC CTCCGTGCGG TCCAACATCC GGGTCACAGT CACAGCCACC
    GCAGTCATCA TCAACCTAGT GGTCATCATC CTCCTGGACG AGGTGTATGG CTGCATAGCC
    CGATGGCTCA CCAAGATCGA GGTCCCAAAG ACGGAGAAAA GCTTTGAGGA GAGGCTGATC
    TTCAAGGCTT TCCTGCTGAA GTTTGTGAAT TCCTACACCC CCATCTTTTA CGTGGCGTTC
    TTCAAAGGCC GGTTTGTTGG ACGCCCGGGC GACTACGTGT ACATTTTCCG TTCCTTCCGA
    ATGGAAGAGT GTGCGCCAGG GGGCTGCCTG ATGGAGCTAT GCATCCAGCT CAGCATCATC
    ATGCTGGGGA AACAGCTGAT CCAGAACAAC CTGTTCGAGA TCGGCATCCC GAAGATGAAG
    AAGCTCATCC GCTACCTGAA GCTGAAGCAG CAGAGCCCCC CTGACCACGA GGAGTGTGTG
    AAGAGGAAAC AGCGGTACGA GGTGGATTAC AACCTGGAGC CCTTCGCGGG CCTCACCCCA
    GAGTACATGG AAATGATCAT CCAGTTTGGC TTCGTCACCC TGTTTGTCGC CTCCTTCCCC
    CTGGCCCCAC TGTTTGCGCT GCTGAACAAC ATCATCGAGA TCCGCCTGGA CGCCAAAAAG
    TTTGTCACTG AGCTCCGAAG GCCGGTAGCT GTCAGAGCCA AAGACATCGG AATCTGGTAC
    AATATCCTCA GAGGCATTGG GAAGCTTGCT GTCATCATCA ATGCCTTCGT GATCTCCTTC
    ACGTCTGACT TCATCCCGCG CCTGGTGTAC CTCTACATGT ACAGTAAGAA CGGGACCATG
    CACGGCTTCG TCAACCACAC CCTCTCCTCC TTCAACGTCA GTGACTTCCA GAACGGCACG
    GCCCCCAATG ACCCCCTGGA CCTGGGCTAC GAGGTGCAGA TCTGCAGGTA TAAAGACTAC
    CGAGAGCCGC CGTGGTCGGA AAACAAGTAC GACATCTCCA AGGACTTCTG GGCCGTCCTG
    GCAGCCCGGC TGGCGTTTGT CATCGTCTTC CAGAACCTGG TCATGTTCAT GAGCGACTTT
    GTGGACTGGG TCATCCCGGA CATCCCCAAG GACATCAGCC AGCAGATCCA CAAGGAGAAG
    GTGCTCATGG TGGAGCTGTT CATGCGGGAG GAGCAAGACA AGCAGCAGCT GCTGGAAACC
    TGGATGGAGA AGGAGCGGCA GAAGGACGAG CCGCCGTGCA ACCACCACAA CACCAAAGCC
    TGCCCAGACA GCCTCGGCAG CCCAGCCCCC AGCCATGCCT ACCACGGGGG CGTCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011543431.1
    CDS16..2955
    Translation

    Target ORF information:

    RefSeq Version XM_011545129.2
    Organism Homo sapiens(human)
    Definition Homo sapiens anoctamin 1 (ANO1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011545129.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCTGACCA GGCCCTCCCA GCTGCTGAAC TCCTTATCTG TGGACCCTGA TGCCGAGTGC 
    AAGTATGGCC TGTACTTCAG GGACGGCCGG CGCAAGGTGG ACTACATCCT GGTGTACCAT
    CACAAGAGGC CCTCGGGCAA CCGGACCCTG GTCAGGAGGG TGCAGCACAG CGACACCCCC
    TCTGGGGCTC GCAGCGTCAA GCAGGACCAC CCCCTGCCGG GCAAGGGGGC GTCGCTGGAT
    GCAGGCTCGG GGGAGCCCCC GATGGACTAC CACGAGGATG ACAAGCGCTT CCGCAGGGAG
    GAGTACGAGG GCAACCTCCT GGAGGCGGGC CTGGAGCTGG AGCGGGACGA GGACACTAAA
    ATCCACGGAG TCGGGTTTGT GAAAATCCAT GCCCCCTGGA ACGTGCTGTG CAGAGAGGCC
    GAGTTTCTGA AACTGAAGAT GCCGACGAAG AAGATGTACC ACATTAATGA GACCCGTGGC
    CTCCTGAAAA AAATCAACTC TGTGCTCCAG AAAATCACAG ATCCCATCCA GCCCAAAGTG
    GCTGAGCACA GGCCCCAGAC CATGAAGAGA CTCTCCTATC CCTTCTCCCG GGAGAAGCAG
    CATCTATTTG ACTTGTCTGA TAAGGATTCC TTTTTCGACA GCAAAACCCG GAGCACGATT
    GTCTATGAGA TCTTGAAGAG AACGACGTGT ACAAAGGCCA AGTACAGCAT GGGGCAAGGC
    GAGGGAAGAA AGAAGGACTC CGCCCTTCTA AGTAAAAGGC GGAAATGTGG GAAGTATGGC
    ATCACGAGCC TGCTGGCCAA TGGTGTGTAC GCGGCTGCAT ACCCACTGCA CGATGGAGAC
    TACAACGGTG AAAACGTCGA GTTCAACGAC AGAAAACTCC TGTACGAAGA GTGGGCACGC
    TATGGAGTTT TCTATAAGTA CCAGCCCATC GACCTGGTCA GGAAGTATTT TGGGGAGAAG
    ATCGGCCTGT ACTTCGCCTG GCTGGGCGTG TACACCCAGA TGCTCATCCC TGCCTCCATC
    GTGGGAATCA TTGTCTTCCT GTACGGATGC GCCACCATGG ATGAAAACAT CCCCAGCATG
    GAGATGTGTG ACCAGAGACA CAATATCACC ATGTGCCCGC TTTGCGACAA GACCTGCAGC
    TACTGGAAGA TGAGCTCAGC CTGCGCCACG GCCCGCGCCA GCCACCTCTT CGACAACCCC
    GCCACGGTCT TCTTCTCTGT CTTCATGGCC CTCTGGGCTG CCACCTTCAT GGAGCACTGG
    AAGCGGAAAC AGATGCGACT CAACTACCGC TGGGACCTCA CGGGCTTTGA AGAGGAAGAG
    GAGGCTGTCA AGGATCATCC TAGAGCTGAA TACGAAGCCA GAGTCTTGGA GAAGTCTCTG
    AAGAAAGAGT CCAGAAACAA AGAGAAGCGC CGGCATATTC CAGAGGAGTC AACAAACAAA
    TGGAAGCAGA GGGTTAAGAC AGCCATGGCG GGGGTGAAAT TGACTGACAA AGTGAAGCTG
    ACATGGAGAG ATCGGTTCCC AGCCTACCTC ACTAACTTGG TCTCCATCAT CTTCATGATT
    GCAGTGACGT TTGCCATCGT CCTCGGCGTC ATCATCTACA GAATCTCCAT GGCCGCCGCC
    TTGGCCATGA ACTCCTCCCC CTCCGTGCGG TCCAACATCC GGGTCACAGT CACAGCCACC
    GCAGTCATCA TCAACCTAGT GGTCATCATC CTCCTGGACG AGGTGTATGG CTGCATAGCC
    CGATGGCTCA CCAAGATCGA GGTCCCAAAG ACGGAGAAAA GCTTTGAGGA GAGGCTGATC
    TTCAAGGCTT TCCTGCTGAA GTTTGTGAAT TCCTACACCC CCATCTTTTA CGTGGCGTTC
    TTCAAAGGCC GGTTTGTTGG ACGCCCGGGC GACTACGTGT ACATTTTCCG TTCCTTCCGA
    ATGGAAGAGT GTGCGCCAGG GGGCTGCCTG ATGGAGCTAT GCATCCAGCT CAGCATCATC
    ATGCTGGGGA AACAGCTGAT CCAGAACAAC CTGTTCGAGA TCGGCATCCC GAAGATGAAG
    AAGCTCATCC GCTACCTGAA GCTGAAGCAG CAGAGCCCCC CTGACCACGA GGAGTGTGTG
    AAGAGGAAAC AGCGGTACGA GGTGGATTAC AACCTGGAGC CCTTCGCGGG CCTCACCCCA
    GAGTACATGG AAATGATCAT CCAGTTTGGC TTCGTCACCC TGTTTGTCGC CTCCTTCCCC
    CTGGCCCCAC TGTTTGCGCT GCTGAACAAC ATCATCGAGA TCCGCCTGGA CGCCAAAAAG
    TTTGTCACTG AGCTCCGAAG GCCGGTAGCT GTCAGAGCCA AAGACATCGG AATCTGGTAC
    AATATCCTCA GAGGCATTGG GAAGCTTGCT GTCATCATCA ATGCCTTCGT GATCTCCTTC
    ACGTCTGACT TCATCCCGCG CCTGGTGTAC CTCTACATGT ACAGTAAGAA CGGGACCATG
    CACGGCTTCG TCAACCACAC CCTCTCCTCC TTCAACGTCA GTGACTTCCA GAACGGCACG
    GCCCCCAATG ACCCCCTGGA CCTGGGCTAC GAGGTGCAGA TCTGCAGGTA TAAAGACTAC
    CGAGAGCCGC CGTGGTCGGA AAACAAGTAC GACATCTCCA AGGACTTCTG GGCCGTCCTG
    GCAGCCCGGC TGGCGTTTGT CATCGTCTTC CAGAACCTGG TCATGTTCAT GAGCGACTTT
    GTGGACTGGG TCATCCCGGA CATCCCCAAG GACATCAGCC AGCAGATCCA CAAGGAGAAG
    GTGCTCATGG TGGAGCTGTT CATGCGGGAG GAGCAAGACA AGCAGCAGCT GCTGGAAACC
    TGGATGGAGA AGGAGCGGCA GAAGGACGAG CCGCCGTGCA ACCACCACAA CACCAAAGCC
    TGCCCAGACA GCCTCGGCAG CCCAGCCCCC AGCCATGCCT ACCACGGGGG CGTCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92337
    Clone ID Related Accession (Same CDS sequence) XM_017017956.1
    Accession Version XM_017017956.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2958bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product anoctamin-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTATCTGTGG ACCCTGATGC CGAGTGCAAG TATGGCCTGT ACTTCAGGGA CGGCCGGCGC
    AAGGTGGACT ACATCCTGGT GTACCATCAC AAGAGGCCCT CGGGCAACCG GACCCTGGTC
    AGGAGGGTGC AGCACAGCGA CACCCCCTCT GGGGCTCGCA GCGTCAAGCA GGACCACCCC
    CTGCCGGGCA AGGGGGCGTC GCTGGATGCA GGCTCGGGGG AGCCCCCGAT GGACTACCAC
    GAGGATGACA AGCGCTTCCG CAGGGAGGAG TACGAGGGCA ACCTCCTGGA GGCGGGCCTG
    GAGCTGGAGC GGGACGAGGA CACTAAAATC CACGGAGTCG GGTTTGTGAA AATCCATGCC
    CCCTGGAACG TGCTGTGCAG AGAGGCCGAG TTTCTGAAAC TGAAGATGCC GACGAAGAAG
    ATGTACCACA TTAATGAGAC CCGTGGCCTC CTGAAAAAAA TCAACTCTGT GCTCCAGAAA
    ATCACAGATC CCATCCAGCC CAAAGTGGCT GAGCACAGGC CCCAGACCAT GAAGAGACTC
    TCCTATCCCT TCTCCCGGGA GAAGCAGCAT CTATTTGACT TGTCTGATAA GGATTCCTTT
    TTCGACAGCA AAACCCGGAG CACGATTGTC TATGAGATCT TGAAGAGAAC GACGTGTACA
    AAGGCCAAGT ACAGCATGGG CATCACGAGC CTGCTGGCCA ATGGTGTGTA CGCGGCTGCA
    TACCCACTGC ACGATGGAGA CTACAACGGT GAAAACGTCG AGTTCAACGA CAGAAAACTC
    CTGTACGAAG AGTGGGCACG CTATGGAGTT TTCTATAAGT ACCAGCCCAT CGACCTGGTC
    AGGAAGTATT TTGGGGAGAA GATCGGCCTG TACTTCGCCT GGCTGGGCGT GTACACCCAG
    ATGCTCATCC CTGCCTCCAT CGTGGGAATC ATTGTCTTCC TGTACGGATG CGCCACCATG
    GATGAAAACA TCCCCAGCAT GGAGATGTGT GACCAGAGAC ACAATATCAC CATGTGCCCG
    CTTTGCGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGCGCCAC GGCCCGCGCC
    AGCCACCTCT TCGACAACCC CGCCACGGTC TTCTTCTCTG TCTTCATGGC CCTCTGGGCT
    GCCACCTTCA TGGAGCACTG GAAGCGGAAA CAGATGCGAC TCAACTACCG CTGGGACCTC
    ACGGGCTTTG AAGAGGAAGA GGCTGTCAAG GATCATCCTA GAGCTGAATA CGAAGCCAGA
    GTCTTGGAGA AGTCTCTGAA GAAAGAGTCC AGAAACAAAG AGAAGCGCCG GCATATTCCA
    GAGGAGTCAA CAAACAAATG GAAGCAGAGG GTTAAGACAG CCATGGCGGG GGTGAAATTG
    ACTGACAAAG TGAAGCTGAC ATGGAGAGAT CGGTTCCCAG CCTACCTCAC TAACTTGGTC
    TCCATCATCT TCATGATTGC AGTGACGTTT GCCATCGTCC TCGGCGTCAT CATCTACAGA
    ATCTCCATGG CCGCCGCCTT GGCCATGAAC TCCTCCCCCT CCGTGCGGTC CAACATCCGG
    GTCACAGTCA CAGCCACCGC AGTCATCATC AACCTAGTGG TCATCATCCT CCTGGACGAG
    GTGTATGGCT GCATAGCCCG ATGGCTCACC AAGATCGAGG TCCCAAAGAC GGAGAAAAGC
    TTTGAGGAGA GGCTGATCTT CAAGGCTTTC CTGCTGAAGT TTGTGAATTC CTACACCCCC
    ATCTTTTACG TGGCGTTCTT CAAAGGCCGG TTTGTTGGAC GCCCGGGCGA CTACGTGTAC
    ATTTTCCGTT CCTTCCGAAT GGAAGAGTGT GCGCCAGGGG GCTGCCTGAT GGAGCTATGC
    ATCCAGCTCA GCATCATCAT GCTGGGGAAA CAGCTGATCC AGAACAACCT GTTCGAGATC
    GGCATCCCGA AGATGAAGAA GCTCATCCGC TACCTGAAGC TGAAGCAGCA GAGCCCCCCT
    GACCACGAGG AGTGTGTGAA GAGGAAACAG CGGTACGAGG TGGATTACAA CCTGGAGCCC
    TTCGCGGGCC TCACCCCAGA GTACATGGAA ATGATCATCC AGTTTGGCTT CGTCACCCTG
    TTTGTCGCCT CCTTCCCCCT GGCCCCACTG TTTGCGCTGC TGAACAACAT CATCGAGATC
    CGCCTGGACG CCAAAAAGTT TGTCACTGAG CTCCGAAGGC CGGTAGCTGT CAGAGCCAAA
    GACATCGGAA TCTGGTACAA TATCCTCAGA GGCATTGGGA AGCTTGCTGT CATCATCAAT
    GCCTTCGTGA TCTCCTTCAC GTCTGACTTC ATCCCGCGCC TGGTGTACCT CTACATGTAC
    AGTAAGAACG GGACCATGCA CGGCTTCGTC AACCACACCC TCTCCTCCTT CAACGTCAGT
    GACTTCCAGA ACGGCACGGC CCCCAATGAC CCCCTGGACC TGGGCTACGA GGTGCAGATC
    TGCAGGTATA AAGACTACCG AGAGCCGCCG TGGTCGGAAA ACAAGTACGA CATCTCCAAG
    GACTTCTGGG CCGTCCTGGC AGCCCGGCTG GCGTTTGTCA TCGTCTTCCA GAACCTGGTC
    ATGTTCATGA GCGACTTTGT GGACTGGGTC ATCCCGGACA TCCCCAAGGA CATCAGCCAG
    CAGATCCACA AGGAGAAGGT GCTCATGGTG GAGCTGTTCA TGCGGGAGGA GCAAGACAAG
    CAGCAGCTGC TGGAAACCTG GATGGAGAAG GAGCGGCAGA AGGACGAGCC GCCGTGCAAC
    CACCACAACA CCAAAGCCTG CCCAGACAGC CTCGGCAGCC CAGCCCCCAG CCATGCCTAC
    CACGGGGGCG TCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016873445.1
    CDS14..2971
    Translation

    Target ORF information:

    RefSeq Version XM_017017956.1
    Organism Homo sapiens(human)
    Definition Homo sapiens anoctamin 1 (ANO1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017017956.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTATCTGTGG ACCCTGATGC CGAGTGCAAG TATGGCCTGT ACTTCAGGGA CGGCCGGCGC
    AAGGTGGACT ACATCCTGGT GTACCATCAC AAGAGGCCCT CGGGCAACCG GACCCTGGTC
    AGGAGGGTGC AGCACAGCGA CACCCCCTCT GGGGCTCGCA GCGTCAAGCA GGACCACCCC
    CTGCCGGGCA AGGGGGCGTC GCTGGATGCA GGCTCGGGGG AGCCCCCGAT GGACTACCAC
    GAGGATGACA AGCGCTTCCG CAGGGAGGAG TACGAGGGCA ACCTCCTGGA GGCGGGCCTG
    GAGCTGGAGC GGGACGAGGA CACTAAAATC CACGGAGTCG GGTTTGTGAA AATCCATGCC
    CCCTGGAACG TGCTGTGCAG AGAGGCCGAG TTTCTGAAAC TGAAGATGCC GACGAAGAAG
    ATGTACCACA TTAATGAGAC CCGTGGCCTC CTGAAAAAAA TCAACTCTGT GCTCCAGAAA
    ATCACAGATC CCATCCAGCC CAAAGTGGCT GAGCACAGGC CCCAGACCAT GAAGAGACTC
    TCCTATCCCT TCTCCCGGGA GAAGCAGCAT CTATTTGACT TGTCTGATAA GGATTCCTTT
    TTCGACAGCA AAACCCGGAG CACGATTGTC TATGAGATCT TGAAGAGAAC GACGTGTACA
    AAGGCCAAGT ACAGCATGGG CATCACGAGC CTGCTGGCCA ATGGTGTGTA CGCGGCTGCA
    TACCCACTGC ACGATGGAGA CTACAACGGT GAAAACGTCG AGTTCAACGA CAGAAAACTC
    CTGTACGAAG AGTGGGCACG CTATGGAGTT TTCTATAAGT ACCAGCCCAT CGACCTGGTC
    AGGAAGTATT TTGGGGAGAA GATCGGCCTG TACTTCGCCT GGCTGGGCGT GTACACCCAG
    ATGCTCATCC CTGCCTCCAT CGTGGGAATC ATTGTCTTCC TGTACGGATG CGCCACCATG
    GATGAAAACA TCCCCAGCAT GGAGATGTGT GACCAGAGAC ACAATATCAC CATGTGCCCG
    CTTTGCGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGCGCCAC GGCCCGCGCC
    AGCCACCTCT TCGACAACCC CGCCACGGTC TTCTTCTCTG TCTTCATGGC CCTCTGGGCT
    GCCACCTTCA TGGAGCACTG GAAGCGGAAA CAGATGCGAC TCAACTACCG CTGGGACCTC
    ACGGGCTTTG AAGAGGAAGA GGCTGTCAAG GATCATCCTA GAGCTGAATA CGAAGCCAGA
    GTCTTGGAGA AGTCTCTGAA GAAAGAGTCC AGAAACAAAG AGAAGCGCCG GCATATTCCA
    GAGGAGTCAA CAAACAAATG GAAGCAGAGG GTTAAGACAG CCATGGCGGG GGTGAAATTG
    ACTGACAAAG TGAAGCTGAC ATGGAGAGAT CGGTTCCCAG CCTACCTCAC TAACTTGGTC
    TCCATCATCT TCATGATTGC AGTGACGTTT GCCATCGTCC TCGGCGTCAT CATCTACAGA
    ATCTCCATGG CCGCCGCCTT GGCCATGAAC TCCTCCCCCT CCGTGCGGTC CAACATCCGG
    GTCACAGTCA CAGCCACCGC AGTCATCATC AACCTAGTGG TCATCATCCT CCTGGACGAG
    GTGTATGGCT GCATAGCCCG ATGGCTCACC AAGATCGAGG TCCCAAAGAC GGAGAAAAGC
    TTTGAGGAGA GGCTGATCTT CAAGGCTTTC CTGCTGAAGT TTGTGAATTC CTACACCCCC
    ATCTTTTACG TGGCGTTCTT CAAAGGCCGG TTTGTTGGAC GCCCGGGCGA CTACGTGTAC
    ATTTTCCGTT CCTTCCGAAT GGAAGAGTGT GCGCCAGGGG GCTGCCTGAT GGAGCTATGC
    ATCCAGCTCA GCATCATCAT GCTGGGGAAA CAGCTGATCC AGAACAACCT GTTCGAGATC
    GGCATCCCGA AGATGAAGAA GCTCATCCGC TACCTGAAGC TGAAGCAGCA GAGCCCCCCT
    GACCACGAGG AGTGTGTGAA GAGGAAACAG CGGTACGAGG TGGATTACAA CCTGGAGCCC
    TTCGCGGGCC TCACCCCAGA GTACATGGAA ATGATCATCC AGTTTGGCTT CGTCACCCTG
    TTTGTCGCCT CCTTCCCCCT GGCCCCACTG TTTGCGCTGC TGAACAACAT CATCGAGATC
    CGCCTGGACG CCAAAAAGTT TGTCACTGAG CTCCGAAGGC CGGTAGCTGT CAGAGCCAAA
    GACATCGGAA TCTGGTACAA TATCCTCAGA GGCATTGGGA AGCTTGCTGT CATCATCAAT
    GCCTTCGTGA TCTCCTTCAC GTCTGACTTC ATCCCGCGCC TGGTGTACCT CTACATGTAC
    AGTAAGAACG GGACCATGCA CGGCTTCGTC AACCACACCC TCTCCTCCTT CAACGTCAGT
    GACTTCCAGA ACGGCACGGC CCCCAATGAC CCCCTGGACC TGGGCTACGA GGTGCAGATC
    TGCAGGTATA AAGACTACCG AGAGCCGCCG TGGTCGGAAA ACAAGTACGA CATCTCCAAG
    GACTTCTGGG CCGTCCTGGC AGCCCGGCTG GCGTTTGTCA TCGTCTTCCA GAACCTGGTC
    ATGTTCATGA GCGACTTTGT GGACTGGGTC ATCCCGGACA TCCCCAAGGA CATCAGCCAG
    CAGATCCACA AGGAGAAGGT GCTCATGGTG GAGCTGTTCA TGCGGGAGGA GCAAGACAAG
    CAGCAGCTGC TGGAAACCTG GATGGAGAAG GAGCGGCAGA AGGACGAGCC GCCGTGCAAC
    CACCACAACA CCAAAGCCTG CCCAGACAGC CTCGGCAGCC CAGCCCCCAG CCATGCCTAC
    CACGGGGGCG TCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92338
    Clone ID Related Accession (Same CDS sequence) XM_017017957.1
    Accession Version XM_017017957.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product anoctamin-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTATCTGTGG ACCCTGATGC CGAGTGCAAG TATGGCCTGT ACTTCAGGGA CGGCCGGCGC
    AAGGTGGACT ACATCCTGGT GTACCATCAC AAGAGGCCCT CGGGCAACCG GACCCTGGTC
    AGGAGGGTGC AGCACAGCGA CACCCCCTCT GGGGCTCGCA GCGTCAAGCA GGACCACCCC
    CTGCCGGGCA AGGGGGCGTC GCTGGATGCA GGCTCGGGGG AGCCCCCGAT GGACTACCAC
    GAGGATGACA AGCGCTTCCG CAGGGAGGAG TACGAGGGCA ACCTCCTGGA GGCGGGCCTG
    GAGCTGGAGC GGGACGAGGA CACTAAAATC CACGGAGTCG GGTTTGTGAA AATCCATGCC
    CCCTGGAACG TGCTGTGCAG AGAGGCCGAG TTTCTGAAAC TGAAGATGCC GACGAAGAAG
    ATGTACCACA TTAATGAGAC CCGTGGCCTC CTGAAAAAAA TCAACTCTGT GCTCCAGAAA
    ATCACAGATC CCATCCAGCC CAAAGTGGCT GAGCACAGGC CCCAGACCAT GAAGAGACTC
    TCCTATCCCT TCTCCCGGGA GAAGCAGCAT CTATTTGACT TGTCTGATAA GGATTCCTTT
    TTCGACAGCA AAACCCGGAG CACGATTGTC TATGAGATCT TGAAGAGAAC GACGTGTACA
    AAGGCCAAGT ACAGCATGGG CATCACGAGC CTGCTGGCCA ATGGTGTGTA CGCGGCTGCA
    TACCCACTGC ACGATGGAGA CTACAACGGT GAAAACGTCG AGTTCAACGA CAGAAAACTC
    CTGTACGAAG AGTGGGCACG CTATGGAGTT TTCTATAAGT ACCAGCCCAT CGACCTGGTC
    AGGAAGTATT TTGGGGAGAA GATCGGCCTG TACTTCGCCT GGCTGGGCGT GTACACCCAG
    ATGCTCATCC CTGCCTCCAT CGTGGGAATC ATTGTCTTCC TGTACGGATG CGCCACCATG
    GATGAAAACA TCCCCAGCAT GGAGATGTGT GACCAGAGAC ACAATATCAC CATGTGCCCG
    CTTTGCGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGCGCCAC GGCCCGCGCC
    AGCCACCTCT TCGACAACCC CGCCACGGTC TTCTTCTCTG TCTTCATGGC CCTCTGGGCT
    GCCACCTTCA TGGAGCACTG GAAGCGGAAA CAGATGCGAC TCAACTACCG CTGGGACCTC
    ACGGGCTTTG AAGAGGAAGA GGATCATCCT AGAGCTGAAT ACGAAGCCAG AGTCTTGGAG
    AAGTCTCTGA AGAAAGAGTC CAGAAACAAA GAGAAGCGCC GGCATATTCC AGAGGAGTCA
    ACAAACAAAT GGAAGCAGAG GGTTAAGACA GCCATGGCGG GGGTGAAATT GACTGACAAA
    GTGAAGCTGA CATGGAGAGA TCGGTTCCCA GCCTACCTCA CTAACTTGGT CTCCATCATC
    TTCATGATTG CAGTGACGTT TGCCATCGTC CTCGGCGTCA TCATCTACAG AATCTCCATG
    GCCGCCGCCT TGGCCATGAA CTCCTCCCCC TCCGTGCGGT CCAACATCCG GGTCACAGTC
    ACAGCCACCG CAGTCATCAT CAACCTAGTG GTCATCATCC TCCTGGACGA GGTGTATGGC
    TGCATAGCCC GATGGCTCAC CAAGATCGAG GTCCCAAAGA CGGAGAAAAG CTTTGAGGAG
    AGGCTGATCT TCAAGGCTTT CCTGCTGAAG TTTGTGAATT CCTACACCCC CATCTTTTAC
    GTGGCGTTCT TCAAAGGCCG GTTTGTTGGA CGCCCGGGCG ACTACGTGTA CATTTTCCGT
    TCCTTCCGAA TGGAAGAGTG TGCGCCAGGG GGCTGCCTGA TGGAGCTATG CATCCAGCTC
    AGCATCATCA TGCTGGGGAA ACAGCTGATC CAGAACAACC TGTTCGAGAT CGGCATCCCG
    AAGATGAAGA AGCTCATCCG CTACCTGAAG CTGAAGCAGC AGAGCCCCCC TGACCACGAG
    GAGTGTGTGA AGAGGAAACA GCGGTACGAG GTGGATTACA ACCTGGAGCC CTTCGCGGGC
    CTCACCCCAG AGTACATGGA AATGATCATC CAGTTTGGCT TCGTCACCCT GTTTGTCGCC
    TCCTTCCCCC TGGCCCCACT GTTTGCGCTG CTGAACAACA TCATCGAGAT CCGCCTGGAC
    GCCAAAAAGT TTGTCACTGA GCTCCGAAGG CCGGTAGCTG TCAGAGCCAA AGACATCGGA
    ATCTGGTACA ATATCCTCAG AGGCATTGGG AAGCTTGCTG TCATCATCAA TGCCTTCGTG
    ATCTCCTTCA CGTCTGACTT CATCCCGCGC CTGGTGTACC TCTACATGTA CAGTAAGAAC
    GGGACCATGC ACGGCTTCGT CAACCACACC CTCTCCTCCT TCAACGTCAG TGACTTCCAG
    AACGGCACGG CCCCCAATGA CCCCCTGGAC CTGGGCTACG AGGTGCAGAT CTGCAGGTAT
    AAAGACTACC GAGAGCCGCC GTGGTCGGAA AACAAGTACG ACATCTCCAA GGACTTCTGG
    GCCGTCCTGG CAGCCCGGCT GGCGTTTGTC ATCGTCTTCC AGAACCTGGT CATGTTCATG
    AGCGACTTTG TGGACTGGGT CATCCCGGAC ATCCCCAAGG ACATCAGCCA GCAGATCCAC
    AAGGAGAAGG TGCTCATGGT GGAGCTGTTC ATGCGGGAGG AGCAAGACAA GCAGCAGCTG
    CTGGAAACCT GGATGGAGAA GGAGCGGCAG AAGGACGAGC CGCCGTGCAA CCACCACAAC
    ACCAAAGCCT GCCCAGACAG CCTCGGCAGC CCAGCCCCCA GCCATGCCTA CCACGGGGGC
    GTCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016873446.1
    CDS14..2962
    Translation

    Target ORF information:

    RefSeq Version XM_017017957.1
    Organism Homo sapiens(human)
    Definition Homo sapiens anoctamin 1 (ANO1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017017957.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTATCTGTGG ACCCTGATGC CGAGTGCAAG TATGGCCTGT ACTTCAGGGA CGGCCGGCGC
    AAGGTGGACT ACATCCTGGT GTACCATCAC AAGAGGCCCT CGGGCAACCG GACCCTGGTC
    AGGAGGGTGC AGCACAGCGA CACCCCCTCT GGGGCTCGCA GCGTCAAGCA GGACCACCCC
    CTGCCGGGCA AGGGGGCGTC GCTGGATGCA GGCTCGGGGG AGCCCCCGAT GGACTACCAC
    GAGGATGACA AGCGCTTCCG CAGGGAGGAG TACGAGGGCA ACCTCCTGGA GGCGGGCCTG
    GAGCTGGAGC GGGACGAGGA CACTAAAATC CACGGAGTCG GGTTTGTGAA AATCCATGCC
    CCCTGGAACG TGCTGTGCAG AGAGGCCGAG TTTCTGAAAC TGAAGATGCC GACGAAGAAG
    ATGTACCACA TTAATGAGAC CCGTGGCCTC CTGAAAAAAA TCAACTCTGT GCTCCAGAAA
    ATCACAGATC CCATCCAGCC CAAAGTGGCT GAGCACAGGC CCCAGACCAT GAAGAGACTC
    TCCTATCCCT TCTCCCGGGA GAAGCAGCAT CTATTTGACT TGTCTGATAA GGATTCCTTT
    TTCGACAGCA AAACCCGGAG CACGATTGTC TATGAGATCT TGAAGAGAAC GACGTGTACA
    AAGGCCAAGT ACAGCATGGG CATCACGAGC CTGCTGGCCA ATGGTGTGTA CGCGGCTGCA
    TACCCACTGC ACGATGGAGA CTACAACGGT GAAAACGTCG AGTTCAACGA CAGAAAACTC
    CTGTACGAAG AGTGGGCACG CTATGGAGTT TTCTATAAGT ACCAGCCCAT CGACCTGGTC
    AGGAAGTATT TTGGGGAGAA GATCGGCCTG TACTTCGCCT GGCTGGGCGT GTACACCCAG
    ATGCTCATCC CTGCCTCCAT CGTGGGAATC ATTGTCTTCC TGTACGGATG CGCCACCATG
    GATGAAAACA TCCCCAGCAT GGAGATGTGT GACCAGAGAC ACAATATCAC CATGTGCCCG
    CTTTGCGACA AGACCTGCAG CTACTGGAAG ATGAGCTCAG CCTGCGCCAC GGCCCGCGCC
    AGCCACCTCT TCGACAACCC CGCCACGGTC TTCTTCTCTG TCTTCATGGC CCTCTGGGCT
    GCCACCTTCA TGGAGCACTG GAAGCGGAAA CAGATGCGAC TCAACTACCG CTGGGACCTC
    ACGGGCTTTG AAGAGGAAGA GGATCATCCT AGAGCTGAAT ACGAAGCCAG AGTCTTGGAG
    AAGTCTCTGA AGAAAGAGTC CAGAAACAAA GAGAAGCGCC GGCATATTCC AGAGGAGTCA
    ACAAACAAAT GGAAGCAGAG GGTTAAGACA GCCATGGCGG GGGTGAAATT GACTGACAAA
    GTGAAGCTGA CATGGAGAGA TCGGTTCCCA GCCTACCTCA CTAACTTGGT CTCCATCATC
    TTCATGATTG CAGTGACGTT TGCCATCGTC CTCGGCGTCA TCATCTACAG AATCTCCATG
    GCCGCCGCCT TGGCCATGAA CTCCTCCCCC TCCGTGCGGT CCAACATCCG GGTCACAGTC
    ACAGCCACCG CAGTCATCAT CAACCTAGTG GTCATCATCC TCCTGGACGA GGTGTATGGC
    TGCATAGCCC GATGGCTCAC CAAGATCGAG GTCCCAAAGA CGGAGAAAAG CTTTGAGGAG
    AGGCTGATCT TCAAGGCTTT CCTGCTGAAG TTTGTGAATT CCTACACCCC CATCTTTTAC
    GTGGCGTTCT TCAAAGGCCG GTTTGTTGGA CGCCCGGGCG ACTACGTGTA CATTTTCCGT
    TCCTTCCGAA TGGAAGAGTG TGCGCCAGGG GGCTGCCTGA TGGAGCTATG CATCCAGCTC
    AGCATCATCA TGCTGGGGAA ACAGCTGATC CAGAACAACC TGTTCGAGAT CGGCATCCCG
    AAGATGAAGA AGCTCATCCG CTACCTGAAG CTGAAGCAGC AGAGCCCCCC TGACCACGAG
    GAGTGTGTGA AGAGGAAACA GCGGTACGAG GTGGATTACA ACCTGGAGCC CTTCGCGGGC
    CTCACCCCAG AGTACATGGA AATGATCATC CAGTTTGGCT TCGTCACCCT GTTTGTCGCC
    TCCTTCCCCC TGGCCCCACT GTTTGCGCTG CTGAACAACA TCATCGAGAT CCGCCTGGAC
    GCCAAAAAGT TTGTCACTGA GCTCCGAAGG CCGGTAGCTG TCAGAGCCAA AGACATCGGA
    ATCTGGTACA ATATCCTCAG AGGCATTGGG AAGCTTGCTG TCATCATCAA TGCCTTCGTG
    ATCTCCTTCA CGTCTGACTT CATCCCGCGC CTGGTGTACC TCTACATGTA CAGTAAGAAC
    GGGACCATGC ACGGCTTCGT CAACCACACC CTCTCCTCCT TCAACGTCAG TGACTTCCAG
    AACGGCACGG CCCCCAATGA CCCCCTGGAC CTGGGCTACG AGGTGCAGAT CTGCAGGTAT
    AAAGACTACC GAGAGCCGCC GTGGTCGGAA AACAAGTACG ACATCTCCAA GGACTTCTGG
    GCCGTCCTGG CAGCCCGGCT GGCGTTTGTC ATCGTCTTCC AGAACCTGGT CATGTTCATG
    AGCGACTTTG TGGACTGGGT CATCCCGGAC ATCCCCAAGG ACATCAGCCA GCAGATCCAC
    AAGGAGAAGG TGCTCATGGT GGAGCTGTTC ATGCGGGAGG AGCAAGACAA GCAGCAGCTG
    CTGGAAACCT GGATGGAGAA GGAGCGGCAG AAGGACGAGC CGCCGTGCAA CCACCACAAC
    ACCAAAGCCT GCCCAGACAG CCTCGGCAGC CCAGCCCCCA GCCATGCCTA CCACGGGGGC
    GTCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu68983
    Clone ID Related Accession (Same CDS sequence) XM_011545121.2
    Accession Version XM_011545121.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3027bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product anoctamin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545121.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTATCTGTGG ACCCTGATGC CGAGTGCAAG TATGGCCTGT ACTTCAGGGA CGGCCGGCGC
    AAGGTGGACT ACATCCTGGT GTACCATCAC AAGAGGCCCT CGGGCAACCG GACCCTGGTC
    AGGAGGGTGC AGCACAGCGA CACCCCCTCT GGGGCTCGCA GCGTCAAGCA GGACCACCCC
    CTGCCGGGCA AGGGGGCGTC GCTGGATGCA GGCTCGGGGG AGCCCCCGAT GGACTACCAC
    GAGGATGACA AGCGCTTCCG CAGGGAGGAG TACGAGGGCA ACCTCCTGGA GGCGGGCCTG
    GAGCTGGAGC GGGACGAGGA CACTAAAATC CACGGAGTCG GGTTTGTGAA AATCCATGCC
    CCCTGGAACG TGCTGTGCAG AGAGGCCGAG TTTCTGAAAC TGAAGATGCC GACGAAGAAG
    ATGTACCACA TTAATGAGAC CCGTGGCCTC CTGAAAAAAA TCAACTCTGT GCTCCAGAAA
    ATCACAGATC CCATCCAGCC CAAAGTGGCT GAGCACAGGC CCCAGACCAT GAAGAGACTC
    TCCTATCCCT TCTCCCGGGA GAAGCAGCAT CTATTTGACT TGTCTGATAA GGATTCCTTT
    TTCGACAGCA AAACCCGGAG CACGATTGTC TATGAGATCT TGAAGAGAAC GACGTGTACA
    AAGGCCAAGT ACAGCATGGG GCAAGGCGAG GGAAGAAAGA AGGACTCCGC CCTTCTAAGT
    AAAAGGCGGA AATGTGGGAA GTATGGCATC ACGAGCCTGC TGGCCAATGG TGTGTACGCG
    GCTGCATACC CACTGCACGA TGGAGACTAC AACGGTGAAA ACGTCGAGTT CAACGACAGA
    AAACTCCTGT ACGAAGAGTG GGCACGCTAT GGAGTTTTCT ATAAGTACCA GCCCATCGAC
    CTGGTCAGGA AGTATTTTGG GGAGAAGATC GGCCTGTACT TCGCCTGGCT GGGCGTGTAC
    ACCCAGATGC TCATCCCTGC CTCCATCGTG GGAATCATTG TCTTCCTGTA CGGATGCGCC
    ACCATGGATG AAAACATCCC CAGCATGGAG ATGTGTGACC AGAGACACAA TATCACCATG
    TGCCCGCTTT GCGACAAGAC CTGCAGCTAC TGGAAGATGA GCTCAGCCTG CGCCACGGCC
    CGCGCCAGCC ACCTCTTCGA CAACCCCGCC ACGGTCTTCT TCTCTGTCTT CATGGCCCTC
    TGGGCTGCCA CCTTCATGGA GCACTGGAAG CGGAAACAGA TGCGACTCAA CTACCGCTGG
    GACCTCACGG GCTTTGAAGA GGAAGAGGAG GCTGTCAAGG ATCATCCTAG AGCTGAATAC
    GAAGCCAGAG TCTTGGAGAA GTCTCTGAAG AAAGAGTCCA GAAACAAAGA GAAGCGCCGG
    CATATTCCAG AGGAGTCAAC AAACAAATGG AAGCAGAGGG TTAAGACAGC CATGGCGGGG
    GTGAAATTGA CTGACAAAGT GAAGCTGACA TGGAGAGATC GGTTCCCAGC CTACCTCACT
    AACTTGGTCT CCATCATCTT CATGATTGCA GTGACGTTTG CCATCGTCCT CGGCGTCATC
    ATCTACAGAA TCTCCATGGC CGCCGCCTTG GCCATGAACT CCTCCCCCTC CGTGCGGTCC
    AACATCCGGG TCACAGTCAC AGCCACCGCA GTCATCATCA ACCTAGTGGT CATCATCCTC
    CTGGACGAGG TGTATGGCTG CATAGCCCGA TGGCTCACCA AGATCGAGGT CCCAAAGACG
    GAGAAAAGCT TTGAGGAGAG GCTGATCTTC AAGGCTTTCC TGCTGAAGTT TGTGAATTCC
    TACACCCCCA TCTTTTACGT GGCGTTCTTC AAAGGCCGGT TTGTTGGACG CCCGGGCGAC
    TACGTGTACA TTTTCCGTTC CTTCCGAATG GAAGAGTGTG CGCCAGGGGG CTGCCTGATG
    GAGCTATGCA TCCAGCTCAG CATCATCATG CTGGGGAAAC AGCTGATCCA GAACAACCTG
    TTCGAGATCG GCATCCCGAA GATGAAGAAG CTCATCCGCT ACCTGAAGCT GAAGCAGCAG
    AGCCCCCCTG ACCACGAGGA GTGTGTGAAG AGGAAACAGC GGTACGAGGT GGATTACAAC
    CTGGAGCCCT TCGCGGGCCT CACCCCAGAG TACATGGAAA TGATCATCCA GTTTGGCTTC
    GTCACCCTGT TTGTCGCCTC CTTCCCCCTG GCCCCACTGT TTGCGCTGCT GAACAACATC
    ATCGAGATCC GCCTGGACGC CAAAAAGTTT GTCACTGAGC TCCGAAGGCC GGTAGCTGTC
    AGAGCCAAAG ACATCGGAAT CTGGTACAAT ATCCTCAGAG GCATTGGGAA GCTTGCTGTC
    ATCATCAATG CCTTCGTGAT CTCCTTCACG TCTGACTTCA TCCCGCGCCT GGTGTACCTC
    TACATGTACA GTAAGAACGG GACCATGCAC GGCTTCGTCA ACCACACCCT CTCCTCCTTC
    AACGTCAGTG ACTTCCAGAA CGGCACGGCC CCCAATGACC CCCTGGACCT GGGCTACGAG
    GTGCAGATCT GCAGGTATAA AGACTACCGA GAGCCGCCGT GGTCGGAAAA CAAGTACGAC
    ATCTCCAAGG ACTTCTGGGC CGTCCTGGCA GCCCGGCTGG CGTTTGTCAT CGTCTTCCAG
    AACCTGGTCA TGTTCATGAG CGACTTTGTG GACTGGGTCA TCCCGGACAT CCCCAAGGAC
    ATCAGCCAGC AGATCCACAA GGAGAAGGTG CTCATGGTGG AGCTGTTCAT GCGGGAGGAG
    CAAGACAAGC AGCAGCTGCT GGAAACCTGG ATGGAGAAGG AGCGGCAGAA GGACGAGCCG
    CCGTGCAACC ACCACAACAC CAAAGCCTGC CCAGACAGCC TCGGCAGCCC AGCCCCCAGC
    CATGCCTACC ACGGGGGCGT CCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011543423.1
    CDS14..3040
    Translation

    Target ORF information:

    RefSeq Version XM_011545121.2
    Organism Homo sapiens(human)
    Definition Homo sapiens anoctamin 1 (ANO1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011545121.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTATCTGTGG ACCCTGATGC CGAGTGCAAG TATGGCCTGT ACTTCAGGGA CGGCCGGCGC
    AAGGTGGACT ACATCCTGGT GTACCATCAC AAGAGGCCCT CGGGCAACCG GACCCTGGTC
    AGGAGGGTGC AGCACAGCGA CACCCCCTCT GGGGCTCGCA GCGTCAAGCA GGACCACCCC
    CTGCCGGGCA AGGGGGCGTC GCTGGATGCA GGCTCGGGGG AGCCCCCGAT GGACTACCAC
    GAGGATGACA AGCGCTTCCG CAGGGAGGAG TACGAGGGCA ACCTCCTGGA GGCGGGCCTG
    GAGCTGGAGC GGGACGAGGA CACTAAAATC CACGGAGTCG GGTTTGTGAA AATCCATGCC
    CCCTGGAACG TGCTGTGCAG AGAGGCCGAG TTTCTGAAAC TGAAGATGCC GACGAAGAAG
    ATGTACCACA TTAATGAGAC CCGTGGCCTC CTGAAAAAAA TCAACTCTGT GCTCCAGAAA
    ATCACAGATC CCATCCAGCC CAAAGTGGCT GAGCACAGGC CCCAGACCAT GAAGAGACTC
    TCCTATCCCT TCTCCCGGGA GAAGCAGCAT CTATTTGACT TGTCTGATAA GGATTCCTTT
    TTCGACAGCA AAACCCGGAG CACGATTGTC TATGAGATCT TGAAGAGAAC GACGTGTACA
    AAGGCCAAGT ACAGCATGGG GCAAGGCGAG GGAAGAAAGA AGGACTCCGC CCTTCTAAGT
    AAAAGGCGGA AATGTGGGAA GTATGGCATC ACGAGCCTGC TGGCCAATGG TGTGTACGCG
    GCTGCATACC CACTGCACGA TGGAGACTAC AACGGTGAAA ACGTCGAGTT CAACGACAGA
    AAACTCCTGT ACGAAGAGTG GGCACGCTAT GGAGTTTTCT ATAAGTACCA GCCCATCGAC
    CTGGTCAGGA AGTATTTTGG GGAGAAGATC GGCCTGTACT TCGCCTGGCT GGGCGTGTAC
    ACCCAGATGC TCATCCCTGC CTCCATCGTG GGAATCATTG TCTTCCTGTA CGGATGCGCC
    ACCATGGATG AAAACATCCC CAGCATGGAG ATGTGTGACC AGAGACACAA TATCACCATG
    TGCCCGCTTT GCGACAAGAC CTGCAGCTAC TGGAAGATGA GCTCAGCCTG CGCCACGGCC
    CGCGCCAGCC ACCTCTTCGA CAACCCCGCC ACGGTCTTCT TCTCTGTCTT CATGGCCCTC
    TGGGCTGCCA CCTTCATGGA GCACTGGAAG CGGAAACAGA TGCGACTCAA CTACCGCTGG
    GACCTCACGG GCTTTGAAGA GGAAGAGGAG GCTGTCAAGG ATCATCCTAG AGCTGAATAC
    GAAGCCAGAG TCTTGGAGAA GTCTCTGAAG AAAGAGTCCA GAAACAAAGA GAAGCGCCGG
    CATATTCCAG AGGAGTCAAC AAACAAATGG AAGCAGAGGG TTAAGACAGC CATGGCGGGG
    GTGAAATTGA CTGACAAAGT GAAGCTGACA TGGAGAGATC GGTTCCCAGC CTACCTCACT
    AACTTGGTCT CCATCATCTT CATGATTGCA GTGACGTTTG CCATCGTCCT CGGCGTCATC
    ATCTACAGAA TCTCCATGGC CGCCGCCTTG GCCATGAACT CCTCCCCCTC CGTGCGGTCC
    AACATCCGGG TCACAGTCAC AGCCACCGCA GTCATCATCA ACCTAGTGGT CATCATCCTC
    CTGGACGAGG TGTATGGCTG CATAGCCCGA TGGCTCACCA AGATCGAGGT CCCAAAGACG
    GAGAAAAGCT TTGAGGAGAG GCTGATCTTC AAGGCTTTCC TGCTGAAGTT TGTGAATTCC
    TACACCCCCA TCTTTTACGT GGCGTTCTTC AAAGGCCGGT TTGTTGGACG CCCGGGCGAC
    TACGTGTACA TTTTCCGTTC CTTCCGAATG GAAGAGTGTG CGCCAGGGGG CTGCCTGATG
    GAGCTATGCA TCCAGCTCAG CATCATCATG CTGGGGAAAC AGCTGATCCA GAACAACCTG
    TTCGAGATCG GCATCCCGAA GATGAAGAAG CTCATCCGCT ACCTGAAGCT GAAGCAGCAG
    AGCCCCCCTG ACCACGAGGA GTGTGTGAAG AGGAAACAGC GGTACGAGGT GGATTACAAC
    CTGGAGCCCT TCGCGGGCCT CACCCCAGAG TACATGGAAA TGATCATCCA GTTTGGCTTC
    GTCACCCTGT TTGTCGCCTC CTTCCCCCTG GCCCCACTGT TTGCGCTGCT GAACAACATC
    ATCGAGATCC GCCTGGACGC CAAAAAGTTT GTCACTGAGC TCCGAAGGCC GGTAGCTGTC
    AGAGCCAAAG ACATCGGAAT CTGGTACAAT ATCCTCAGAG GCATTGGGAA GCTTGCTGTC
    ATCATCAATG CCTTCGTGAT CTCCTTCACG TCTGACTTCA TCCCGCGCCT GGTGTACCTC
    TACATGTACA GTAAGAACGG GACCATGCAC GGCTTCGTCA ACCACACCCT CTCCTCCTTC
    AACGTCAGTG ACTTCCAGAA CGGCACGGCC CCCAATGACC CCCTGGACCT GGGCTACGAG
    GTGCAGATCT GCAGGTATAA AGACTACCGA GAGCCGCCGT GGTCGGAAAA CAAGTACGAC
    ATCTCCAAGG ACTTCTGGGC CGTCCTGGCA GCCCGGCTGG CGTTTGTCAT CGTCTTCCAG
    AACCTGGTCA TGTTCATGAG CGACTTTGTG GACTGGGTCA TCCCGGACAT CCCCAAGGAC
    ATCAGCCAGC AGATCCACAA GGAGAAGGTG CTCATGGTGG AGCTGTTCAT GCGGGAGGAG
    CAAGACAAGC AGCAGCTGCT GGAAACCTGG ATGGAGAAGG AGCGGCAGAA GGACGAGCCG
    CCGTGCAACC ACCACAACAC CAAAGCCTGC CCAGACAGCC TCGGCAGCCC AGCCCCCAGC
    CATGCCTACC ACGGGGGCGT CCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu68985
    Clone ID Related Accession (Same CDS sequence) XM_011545123.2
    Accession Version XM_011545123.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3024bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product anoctamin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545123.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTATCTGTGG ACCCTGATGC CGAGTGCAAG TATGGCCTGT ACTTCAGGGA CGGCCGGCGC
    AAGGTGGACT ACATCCTGGT GTACCATCAC AAGAGGCCCT CGGGCAACCG GACCCTGGTC
    AGGAGGGTGC AGCACAGCGA CACCCCCTCT GGGGCTCGCA GCGTCAAGCA GGACCACCCC
    CTGCCGGGCA AGGGGGCGTC GCTGGATGCA GGCTCGGGGG AGCCCCCGAT GGACTACCAC
    GAGGATGACA AGCGCTTCCG CAGGGAGGAG TACGAGGGCA ACCTCCTGGA GGCGGGCCTG
    GAGCTGGAGC GGGACGAGGA CACTAAAATC CACGGAGTCG GGTTTGTGAA AATCCATGCC
    CCCTGGAACG TGCTGTGCAG AGAGGCCGAG TTTCTGAAAC TGAAGATGCC GACGAAGAAG
    ATGTACCACA TTAATGAGAC CCGTGGCCTC CTGAAAAAAA TCAACTCTGT GCTCCAGAAA
    ATCACAGATC CCATCCAGCC CAAAGTGGCT GAGCACAGGC CCCAGACCAT GAAGAGACTC
    TCCTATCCCT TCTCCCGGGA GAAGCAGCAT CTATTTGACT TGTCTGATAA GGATTCCTTT
    TTCGACAGCA AAACCCGGAG CACGATTGTC TATGAGATCT TGAAGAGAAC GACGTGTACA
    AAGGCCAAGT ACAGCATGGG GCAAGGCGAG GGAAGAAAGA AGGACTCCGC CCTTCTAAGT
    AAAAGGCGGA AATGTGGGAA GTATGGCATC ACGAGCCTGC TGGCCAATGG TGTGTACGCG
    GCTGCATACC CACTGCACGA TGGAGACTAC AACGGTGAAA ACGTCGAGTT CAACGACAGA
    AAACTCCTGT ACGAAGAGTG GGCACGCTAT GGAGTTTTCT ATAAGTACCA GCCCATCGAC
    CTGGTCAGGA AGTATTTTGG GGAGAAGATC GGCCTGTACT TCGCCTGGCT GGGCGTGTAC
    ACCCAGATGC TCATCCCTGC CTCCATCGTG GGAATCATTG TCTTCCTGTA CGGATGCGCC
    ACCATGGATG AAAACATCCC CAGCATGGAG ATGTGTGACC AGAGACACAA TATCACCATG
    TGCCCGCTTT GCGACAAGAC CTGCAGCTAC TGGAAGATGA GCTCAGCCTG CGCCACGGCC
    CGCGCCAGCC ACCTCTTCGA CAACCCCGCC ACGGTCTTCT TCTCTGTCTT CATGGCCCTC
    TGGGCTGCCA CCTTCATGGA GCACTGGAAG CGGAAACAGA TGCGACTCAA CTACCGCTGG
    GACCTCACGG GCTTTGAAGA GGAAGAGGCT GTCAAGGATC ATCCTAGAGC TGAATACGAA
    GCCAGAGTCT TGGAGAAGTC TCTGAAGAAA GAGTCCAGAA ACAAAGAGAA GCGCCGGCAT
    ATTCCAGAGG AGTCAACAAA CAAATGGAAG CAGAGGGTTA AGACAGCCAT GGCGGGGGTG
    AAATTGACTG ACAAAGTGAA GCTGACATGG AGAGATCGGT TCCCAGCCTA CCTCACTAAC
    TTGGTCTCCA TCATCTTCAT GATTGCAGTG ACGTTTGCCA TCGTCCTCGG CGTCATCATC
    TACAGAATCT CCATGGCCGC CGCCTTGGCC ATGAACTCCT CCCCCTCCGT GCGGTCCAAC
    ATCCGGGTCA CAGTCACAGC CACCGCAGTC ATCATCAACC TAGTGGTCAT CATCCTCCTG
    GACGAGGTGT ATGGCTGCAT AGCCCGATGG CTCACCAAGA TCGAGGTCCC AAAGACGGAG
    AAAAGCTTTG AGGAGAGGCT GATCTTCAAG GCTTTCCTGC TGAAGTTTGT GAATTCCTAC
    ACCCCCATCT TTTACGTGGC GTTCTTCAAA GGCCGGTTTG TTGGACGCCC GGGCGACTAC
    GTGTACATTT TCCGTTCCTT CCGAATGGAA GAGTGTGCGC CAGGGGGCTG CCTGATGGAG
    CTATGCATCC AGCTCAGCAT CATCATGCTG GGGAAACAGC TGATCCAGAA CAACCTGTTC
    GAGATCGGCA TCCCGAAGAT GAAGAAGCTC ATCCGCTACC TGAAGCTGAA GCAGCAGAGC
    CCCCCTGACC ACGAGGAGTG TGTGAAGAGG AAACAGCGGT ACGAGGTGGA TTACAACCTG
    GAGCCCTTCG CGGGCCTCAC CCCAGAGTAC ATGGAAATGA TCATCCAGTT TGGCTTCGTC
    ACCCTGTTTG TCGCCTCCTT CCCCCTGGCC CCACTGTTTG CGCTGCTGAA CAACATCATC
    GAGATCCGCC TGGACGCCAA AAAGTTTGTC ACTGAGCTCC GAAGGCCGGT AGCTGTCAGA
    GCCAAAGACA TCGGAATCTG GTACAATATC CTCAGAGGCA TTGGGAAGCT TGCTGTCATC
    ATCAATGCCT TCGTGATCTC CTTCACGTCT GACTTCATCC CGCGCCTGGT GTACCTCTAC
    ATGTACAGTA AGAACGGGAC CATGCACGGC TTCGTCAACC ACACCCTCTC CTCCTTCAAC
    GTCAGTGACT TCCAGAACGG CACGGCCCCC AATGACCCCC TGGACCTGGG CTACGAGGTG
    CAGATCTGCA GGTATAAAGA CTACCGAGAG CCGCCGTGGT CGGAAAACAA GTACGACATC
    TCCAAGGACT TCTGGGCCGT CCTGGCAGCC CGGCTGGCGT TTGTCATCGT CTTCCAGAAC
    CTGGTCATGT TCATGAGCGA CTTTGTGGAC TGGGTCATCC CGGACATCCC CAAGGACATC
    AGCCAGCAGA TCCACAAGGA GAAGGTGCTC ATGGTGGAGC TGTTCATGCG GGAGGAGCAA
    GACAAGCAGC AGCTGCTGGA AACCTGGATG GAGAAGGAGC GGCAGAAGGA CGAGCCGCCG
    TGCAACCACC ACAACACCAA AGCCTGCCCA GACAGCCTCG GCAGCCCAGC CCCCAGCCAT
    GCCTACCACG GGGGCGTCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011543425.1
    CDS14..3037
    Translation

    Target ORF information:

    RefSeq Version XM_011545123.2
    Organism Homo sapiens(human)
    Definition Homo sapiens anoctamin 1 (ANO1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011545123.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTATCTGTGG ACCCTGATGC CGAGTGCAAG TATGGCCTGT ACTTCAGGGA CGGCCGGCGC
    AAGGTGGACT ACATCCTGGT GTACCATCAC AAGAGGCCCT CGGGCAACCG GACCCTGGTC
    AGGAGGGTGC AGCACAGCGA CACCCCCTCT GGGGCTCGCA GCGTCAAGCA GGACCACCCC
    CTGCCGGGCA AGGGGGCGTC GCTGGATGCA GGCTCGGGGG AGCCCCCGAT GGACTACCAC
    GAGGATGACA AGCGCTTCCG CAGGGAGGAG TACGAGGGCA ACCTCCTGGA GGCGGGCCTG
    GAGCTGGAGC GGGACGAGGA CACTAAAATC CACGGAGTCG GGTTTGTGAA AATCCATGCC
    CCCTGGAACG TGCTGTGCAG AGAGGCCGAG TTTCTGAAAC TGAAGATGCC GACGAAGAAG
    ATGTACCACA TTAATGAGAC CCGTGGCCTC CTGAAAAAAA TCAACTCTGT GCTCCAGAAA
    ATCACAGATC CCATCCAGCC CAAAGTGGCT GAGCACAGGC CCCAGACCAT GAAGAGACTC
    TCCTATCCCT TCTCCCGGGA GAAGCAGCAT CTATTTGACT TGTCTGATAA GGATTCCTTT
    TTCGACAGCA AAACCCGGAG CACGATTGTC TATGAGATCT TGAAGAGAAC GACGTGTACA
    AAGGCCAAGT ACAGCATGGG GCAAGGCGAG GGAAGAAAGA AGGACTCCGC CCTTCTAAGT
    AAAAGGCGGA AATGTGGGAA GTATGGCATC ACGAGCCTGC TGGCCAATGG TGTGTACGCG
    GCTGCATACC CACTGCACGA TGGAGACTAC AACGGTGAAA ACGTCGAGTT CAACGACAGA
    AAACTCCTGT ACGAAGAGTG GGCACGCTAT GGAGTTTTCT ATAAGTACCA GCCCATCGAC
    CTGGTCAGGA AGTATTTTGG GGAGAAGATC GGCCTGTACT TCGCCTGGCT GGGCGTGTAC
    ACCCAGATGC TCATCCCTGC CTCCATCGTG GGAATCATTG TCTTCCTGTA CGGATGCGCC
    ACCATGGATG AAAACATCCC CAGCATGGAG ATGTGTGACC AGAGACACAA TATCACCATG
    TGCCCGCTTT GCGACAAGAC CTGCAGCTAC TGGAAGATGA GCTCAGCCTG CGCCACGGCC
    CGCGCCAGCC ACCTCTTCGA CAACCCCGCC ACGGTCTTCT TCTCTGTCTT CATGGCCCTC
    TGGGCTGCCA CCTTCATGGA GCACTGGAAG CGGAAACAGA TGCGACTCAA CTACCGCTGG
    GACCTCACGG GCTTTGAAGA GGAAGAGGCT GTCAAGGATC ATCCTAGAGC TGAATACGAA
    GCCAGAGTCT TGGAGAAGTC TCTGAAGAAA GAGTCCAGAA ACAAAGAGAA GCGCCGGCAT
    ATTCCAGAGG AGTCAACAAA CAAATGGAAG CAGAGGGTTA AGACAGCCAT GGCGGGGGTG
    AAATTGACTG ACAAAGTGAA GCTGACATGG AGAGATCGGT TCCCAGCCTA CCTCACTAAC
    TTGGTCTCCA TCATCTTCAT GATTGCAGTG ACGTTTGCCA TCGTCCTCGG CGTCATCATC
    TACAGAATCT CCATGGCCGC CGCCTTGGCC ATGAACTCCT CCCCCTCCGT GCGGTCCAAC
    ATCCGGGTCA CAGTCACAGC CACCGCAGTC ATCATCAACC TAGTGGTCAT CATCCTCCTG
    GACGAGGTGT ATGGCTGCAT AGCCCGATGG CTCACCAAGA TCGAGGTCCC AAAGACGGAG
    AAAAGCTTTG AGGAGAGGCT GATCTTCAAG GCTTTCCTGC TGAAGTTTGT GAATTCCTAC
    ACCCCCATCT TTTACGTGGC GTTCTTCAAA GGCCGGTTTG TTGGACGCCC GGGCGACTAC
    GTGTACATTT TCCGTTCCTT CCGAATGGAA GAGTGTGCGC CAGGGGGCTG CCTGATGGAG
    CTATGCATCC AGCTCAGCAT CATCATGCTG GGGAAACAGC TGATCCAGAA CAACCTGTTC
    GAGATCGGCA TCCCGAAGAT GAAGAAGCTC ATCCGCTACC TGAAGCTGAA GCAGCAGAGC
    CCCCCTGACC ACGAGGAGTG TGTGAAGAGG AAACAGCGGT ACGAGGTGGA TTACAACCTG
    GAGCCCTTCG CGGGCCTCAC CCCAGAGTAC ATGGAAATGA TCATCCAGTT TGGCTTCGTC
    ACCCTGTTTG TCGCCTCCTT CCCCCTGGCC CCACTGTTTG CGCTGCTGAA CAACATCATC
    GAGATCCGCC TGGACGCCAA AAAGTTTGTC ACTGAGCTCC GAAGGCCGGT AGCTGTCAGA
    GCCAAAGACA TCGGAATCTG GTACAATATC CTCAGAGGCA TTGGGAAGCT TGCTGTCATC
    ATCAATGCCT TCGTGATCTC CTTCACGTCT GACTTCATCC CGCGCCTGGT GTACCTCTAC
    ATGTACAGTA AGAACGGGAC CATGCACGGC TTCGTCAACC ACACCCTCTC CTCCTTCAAC
    GTCAGTGACT TCCAGAACGG CACGGCCCCC AATGACCCCC TGGACCTGGG CTACGAGGTG
    CAGATCTGCA GGTATAAAGA CTACCGAGAG CCGCCGTGGT CGGAAAACAA GTACGACATC
    TCCAAGGACT TCTGGGCCGT CCTGGCAGCC CGGCTGGCGT TTGTCATCGT CTTCCAGAAC
    CTGGTCATGT TCATGAGCGA CTTTGTGGAC TGGGTCATCC CGGACATCCC CAAGGACATC
    AGCCAGCAGA TCCACAAGGA GAAGGTGCTC ATGGTGGAGC TGTTCATGCG GGAGGAGCAA
    GACAAGCAGC AGCTGCTGGA AACCTGGATG GAGAAGGAGC GGCAGAAGGA CGAGCCGCCG
    TGCAACCACC ACAACACCAA AGCCTGCCCA GACAGCCTCG GCAGCCCAGC CCCCAGCCAT
    GCCTACCACG GGGGCGTCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu68986
    Clone ID Related Accession (Same CDS sequence) XM_011545124.2
    Accession Version XM_011545124.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3015bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product anoctamin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545124.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTATCTGTGG ACCCTGATGC CGAGTGCAAG TATGGCCTGT ACTTCAGGGA CGGCCGGCGC
    AAGGTGGACT ACATCCTGGT GTACCATCAC AAGAGGCCCT CGGGCAACCG GACCCTGGTC
    AGGAGGGTGC AGCACAGCGA CACCCCCTCT GGGGCTCGCA GCGTCAAGCA GGACCACCCC
    CTGCCGGGCA AGGGGGCGTC GCTGGATGCA GGCTCGGGGG AGCCCCCGAT GGACTACCAC
    GAGGATGACA AGCGCTTCCG CAGGGAGGAG TACGAGGGCA ACCTCCTGGA GGCGGGCCTG
    GAGCTGGAGC GGGACGAGGA CACTAAAATC CACGGAGTCG GGTTTGTGAA AATCCATGCC
    CCCTGGAACG TGCTGTGCAG AGAGGCCGAG TTTCTGAAAC TGAAGATGCC GACGAAGAAG
    ATGTACCACA TTAATGAGAC CCGTGGCCTC CTGAAAAAAA TCAACTCTGT GCTCCAGAAA
    ATCACAGATC CCATCCAGCC CAAAGTGGCT GAGCACAGGC CCCAGACCAT GAAGAGACTC
    TCCTATCCCT TCTCCCGGGA GAAGCAGCAT CTATTTGACT TGTCTGATAA GGATTCCTTT
    TTCGACAGCA AAACCCGGAG CACGATTGTC TATGAGATCT TGAAGAGAAC GACGTGTACA
    AAGGCCAAGT ACAGCATGGG GCAAGGCGAG GGAAGAAAGA AGGACTCCGC CCTTCTAAGT
    AAAAGGCGGA AATGTGGGAA GTATGGCATC ACGAGCCTGC TGGCCAATGG TGTGTACGCG
    GCTGCATACC CACTGCACGA TGGAGACTAC AACGGTGAAA ACGTCGAGTT CAACGACAGA
    AAACTCCTGT ACGAAGAGTG GGCACGCTAT GGAGTTTTCT ATAAGTACCA GCCCATCGAC
    CTGGTCAGGA AGTATTTTGG GGAGAAGATC GGCCTGTACT TCGCCTGGCT GGGCGTGTAC
    ACCCAGATGC TCATCCCTGC CTCCATCGTG GGAATCATTG TCTTCCTGTA CGGATGCGCC
    ACCATGGATG AAAACATCCC CAGCATGGAG ATGTGTGACC AGAGACACAA TATCACCATG
    TGCCCGCTTT GCGACAAGAC CTGCAGCTAC TGGAAGATGA GCTCAGCCTG CGCCACGGCC
    CGCGCCAGCC ACCTCTTCGA CAACCCCGCC ACGGTCTTCT TCTCTGTCTT CATGGCCCTC
    TGGGCTGCCA CCTTCATGGA GCACTGGAAG CGGAAACAGA TGCGACTCAA CTACCGCTGG
    GACCTCACGG GCTTTGAAGA GGAAGAGGAT CATCCTAGAG CTGAATACGA AGCCAGAGTC
    TTGGAGAAGT CTCTGAAGAA AGAGTCCAGA AACAAAGAGA AGCGCCGGCA TATTCCAGAG
    GAGTCAACAA ACAAATGGAA GCAGAGGGTT AAGACAGCCA TGGCGGGGGT GAAATTGACT
    GACAAAGTGA AGCTGACATG GAGAGATCGG TTCCCAGCCT ACCTCACTAA CTTGGTCTCC
    ATCATCTTCA TGATTGCAGT GACGTTTGCC ATCGTCCTCG GCGTCATCAT CTACAGAATC
    TCCATGGCCG CCGCCTTGGC CATGAACTCC TCCCCCTCCG TGCGGTCCAA CATCCGGGTC
    ACAGTCACAG CCACCGCAGT CATCATCAAC CTAGTGGTCA TCATCCTCCT GGACGAGGTG
    TATGGCTGCA TAGCCCGATG GCTCACCAAG ATCGAGGTCC CAAAGACGGA GAAAAGCTTT
    GAGGAGAGGC TGATCTTCAA GGCTTTCCTG CTGAAGTTTG TGAATTCCTA CACCCCCATC
    TTTTACGTGG CGTTCTTCAA AGGCCGGTTT GTTGGACGCC CGGGCGACTA CGTGTACATT
    TTCCGTTCCT TCCGAATGGA AGAGTGTGCG CCAGGGGGCT GCCTGATGGA GCTATGCATC
    CAGCTCAGCA TCATCATGCT GGGGAAACAG CTGATCCAGA ACAACCTGTT CGAGATCGGC
    ATCCCGAAGA TGAAGAAGCT CATCCGCTAC CTGAAGCTGA AGCAGCAGAG CCCCCCTGAC
    CACGAGGAGT GTGTGAAGAG GAAACAGCGG TACGAGGTGG ATTACAACCT GGAGCCCTTC
    GCGGGCCTCA CCCCAGAGTA CATGGAAATG ATCATCCAGT TTGGCTTCGT CACCCTGTTT
    GTCGCCTCCT TCCCCCTGGC CCCACTGTTT GCGCTGCTGA ACAACATCAT CGAGATCCGC
    CTGGACGCCA AAAAGTTTGT CACTGAGCTC CGAAGGCCGG TAGCTGTCAG AGCCAAAGAC
    ATCGGAATCT GGTACAATAT CCTCAGAGGC ATTGGGAAGC TTGCTGTCAT CATCAATGCC
    TTCGTGATCT CCTTCACGTC TGACTTCATC CCGCGCCTGG TGTACCTCTA CATGTACAGT
    AAGAACGGGA CCATGCACGG CTTCGTCAAC CACACCCTCT CCTCCTTCAA CGTCAGTGAC
    TTCCAGAACG GCACGGCCCC CAATGACCCC CTGGACCTGG GCTACGAGGT GCAGATCTGC
    AGGTATAAAG ACTACCGAGA GCCGCCGTGG TCGGAAAACA AGTACGACAT CTCCAAGGAC
    TTCTGGGCCG TCCTGGCAGC CCGGCTGGCG TTTGTCATCG TCTTCCAGAA CCTGGTCATG
    TTCATGAGCG ACTTTGTGGA CTGGGTCATC CCGGACATCC CCAAGGACAT CAGCCAGCAG
    ATCCACAAGG AGAAGGTGCT CATGGTGGAG CTGTTCATGC GGGAGGAGCA AGACAAGCAG
    CAGCTGCTGG AAACCTGGAT GGAGAAGGAG CGGCAGAAGG ACGAGCCGCC GTGCAACCAC
    CACAACACCA AAGCCTGCCC AGACAGCCTC GGCAGCCCAG CCCCCAGCCA TGCCTACCAC
    GGGGGCGTCC TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011543426.1
    CDS14..3028
    Translation

    Target ORF information:

    RefSeq Version XM_011545124.2
    Organism Homo sapiens(human)
    Definition Homo sapiens anoctamin 1 (ANO1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011545124.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTATCTGTGG ACCCTGATGC CGAGTGCAAG TATGGCCTGT ACTTCAGGGA CGGCCGGCGC
    AAGGTGGACT ACATCCTGGT GTACCATCAC AAGAGGCCCT CGGGCAACCG GACCCTGGTC
    AGGAGGGTGC AGCACAGCGA CACCCCCTCT GGGGCTCGCA GCGTCAAGCA GGACCACCCC
    CTGCCGGGCA AGGGGGCGTC GCTGGATGCA GGCTCGGGGG AGCCCCCGAT GGACTACCAC
    GAGGATGACA AGCGCTTCCG CAGGGAGGAG TACGAGGGCA ACCTCCTGGA GGCGGGCCTG
    GAGCTGGAGC GGGACGAGGA CACTAAAATC CACGGAGTCG GGTTTGTGAA AATCCATGCC
    CCCTGGAACG TGCTGTGCAG AGAGGCCGAG TTTCTGAAAC TGAAGATGCC GACGAAGAAG
    ATGTACCACA TTAATGAGAC CCGTGGCCTC CTGAAAAAAA TCAACTCTGT GCTCCAGAAA
    ATCACAGATC CCATCCAGCC CAAAGTGGCT GAGCACAGGC CCCAGACCAT GAAGAGACTC
    TCCTATCCCT TCTCCCGGGA GAAGCAGCAT CTATTTGACT TGTCTGATAA GGATTCCTTT
    TTCGACAGCA AAACCCGGAG CACGATTGTC TATGAGATCT TGAAGAGAAC GACGTGTACA
    AAGGCCAAGT ACAGCATGGG GCAAGGCGAG GGAAGAAAGA AGGACTCCGC CCTTCTAAGT
    AAAAGGCGGA AATGTGGGAA GTATGGCATC ACGAGCCTGC TGGCCAATGG TGTGTACGCG
    GCTGCATACC CACTGCACGA TGGAGACTAC AACGGTGAAA ACGTCGAGTT CAACGACAGA
    AAACTCCTGT ACGAAGAGTG GGCACGCTAT GGAGTTTTCT ATAAGTACCA GCCCATCGAC
    CTGGTCAGGA AGTATTTTGG GGAGAAGATC GGCCTGTACT TCGCCTGGCT GGGCGTGTAC
    ACCCAGATGC TCATCCCTGC CTCCATCGTG GGAATCATTG TCTTCCTGTA CGGATGCGCC
    ACCATGGATG AAAACATCCC CAGCATGGAG ATGTGTGACC AGAGACACAA TATCACCATG
    TGCCCGCTTT GCGACAAGAC CTGCAGCTAC TGGAAGATGA GCTCAGCCTG CGCCACGGCC
    CGCGCCAGCC ACCTCTTCGA CAACCCCGCC ACGGTCTTCT TCTCTGTCTT CATGGCCCTC
    TGGGCTGCCA CCTTCATGGA GCACTGGAAG CGGAAACAGA TGCGACTCAA CTACCGCTGG
    GACCTCACGG GCTTTGAAGA GGAAGAGGAT CATCCTAGAG CTGAATACGA AGCCAGAGTC
    TTGGAGAAGT CTCTGAAGAA AGAGTCCAGA AACAAAGAGA AGCGCCGGCA TATTCCAGAG
    GAGTCAACAA ACAAATGGAA GCAGAGGGTT AAGACAGCCA TGGCGGGGGT GAAATTGACT
    GACAAAGTGA AGCTGACATG GAGAGATCGG TTCCCAGCCT ACCTCACTAA CTTGGTCTCC
    ATCATCTTCA TGATTGCAGT GACGTTTGCC ATCGTCCTCG GCGTCATCAT CTACAGAATC
    TCCATGGCCG CCGCCTTGGC CATGAACTCC TCCCCCTCCG TGCGGTCCAA CATCCGGGTC
    ACAGTCACAG CCACCGCAGT CATCATCAAC CTAGTGGTCA TCATCCTCCT GGACGAGGTG
    TATGGCTGCA TAGCCCGATG GCTCACCAAG ATCGAGGTCC CAAAGACGGA GAAAAGCTTT
    GAGGAGAGGC TGATCTTCAA GGCTTTCCTG CTGAAGTTTG TGAATTCCTA CACCCCCATC
    TTTTACGTGG CGTTCTTCAA AGGCCGGTTT GTTGGACGCC CGGGCGACTA CGTGTACATT
    TTCCGTTCCT TCCGAATGGA AGAGTGTGCG CCAGGGGGCT GCCTGATGGA GCTATGCATC
    CAGCTCAGCA TCATCATGCT GGGGAAACAG CTGATCCAGA ACAACCTGTT CGAGATCGGC
    ATCCCGAAGA TGAAGAAGCT CATCCGCTAC CTGAAGCTGA AGCAGCAGAG CCCCCCTGAC
    CACGAGGAGT GTGTGAAGAG GAAACAGCGG TACGAGGTGG ATTACAACCT GGAGCCCTTC
    GCGGGCCTCA CCCCAGAGTA CATGGAAATG ATCATCCAGT TTGGCTTCGT CACCCTGTTT
    GTCGCCTCCT TCCCCCTGGC CCCACTGTTT GCGCTGCTGA ACAACATCAT CGAGATCCGC
    CTGGACGCCA AAAAGTTTGT CACTGAGCTC CGAAGGCCGG TAGCTGTCAG AGCCAAAGAC
    ATCGGAATCT GGTACAATAT CCTCAGAGGC ATTGGGAAGC TTGCTGTCAT CATCAATGCC
    TTCGTGATCT CCTTCACGTC TGACTTCATC CCGCGCCTGG TGTACCTCTA CATGTACAGT
    AAGAACGGGA CCATGCACGG CTTCGTCAAC CACACCCTCT CCTCCTTCAA CGTCAGTGAC
    TTCCAGAACG GCACGGCCCC CAATGACCCC CTGGACCTGG GCTACGAGGT GCAGATCTGC
    AGGTATAAAG ACTACCGAGA GCCGCCGTGG TCGGAAAACA AGTACGACAT CTCCAAGGAC
    TTCTGGGCCG TCCTGGCAGC CCGGCTGGCG TTTGTCATCG TCTTCCAGAA CCTGGTCATG
    TTCATGAGCG ACTTTGTGGA CTGGGTCATC CCGGACATCC CCAAGGACAT CAGCCAGCAG
    ATCCACAAGG AGAAGGTGCT CATGGTGGAG CTGTTCATGC GGGAGGAGCA AGACAAGCAG
    CAGCTGCTGG AAACCTGGAT GGAGAAGGAG CGGCAGAAGG ACGAGCCGCC GTGCAACCAC
    CACAACACCA AAGCCTGCCC AGACAGCCTC GGCAGCCCAG CCCCCAGCCA TGCCTACCAC
    GGGGGCGTCC TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.