chaf1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	chaf1b
Entrez Gene ID	406285
Full Name	chromatin assembly factor 1, subunit B
Synonyms	wu:fd07c09,wu:fe36g06,wu:fv38g09,zgc:56096
General protein information	Preferred Names chromatin assembly factor 1, subunit B Names chromatin assembly factor 1 subunit B fe36g06 gef
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001328129.1	NP_001315058.1	chromatin assembly factor 1 subunit B

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CHAF1B	NP_001008677.2
Danio rerio (zebrafish)	chaf1b	NP_998177.1
Xenopus tropicalis (tropical clawed frog)	chaf1b	XP_002942374.2
Canis lupus familiaris (dog)	CHAF1B	XP_005638957.1
Mus musculus (house mouse)	Chaf1b	NP_082359.1
Drosophila melanogaster (fruit fly)	Caf1-105	NP_610589.2
Homo sapiens (human)	CHAF1B	NP_005432.1
Pan troglodytes (chimpanzee)	CHAF1B	XP_525468.2
	CHAF1B	XP_002803191.1
Arabidopsis thaliana (thale cress)	FAS2	NP_974991.1
Oryza sativa (rice)	Os08g0108200	NP_001060804.1
Bos taurus (cattle)	CHAF1B	NP_001091483.1
Rattus norvegicus (Norway rat)	Chaf1b	NP_001019912.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007544	XP_308335.3

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The phenotype of the good effort mutant zebrafish is retinal degeneration by cell death and is linked to the chromosome assembly factor 1b gene.
Bailey TJ, Hyde DR
Advances in experimental medicine and biology801535-41(2014)

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Huang HT, Kathrein KL, Barton A, Gitlin Z, Huang YH, Ward TP, Hofmann O, Dibiase A, Song A, Tyekucheva S, Hide W, Zhou Y, Zon LI
Nature cell biology15(12)1516-25(2013 Dec)

Mutation of zebrafish caf-1b results in S phase arrest, defective differentiation, and p53-mediated apoptosis during organogenesis.
Fischer S, Prykhozhij S, Rau MJ, Neumann CJ
Cell cycle (Georgetown, Tex.)6(23)2962-9(2007 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following chaf1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chaf1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa15235	NM_001328129.1 Latest version!	Danio rerio chromatin assembly factor 1, subunit B (chaf1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chaf1b ( NM_001328129.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001315058.1 Danio rerio chromatin assembly factor 1, subunit B (chaf1b), mRNA.

Price & Availability↑

CloneID	ODa15235
Clone ID Related Accession (Same CDS sequence)	NM_001328129.1
Accession Version	NM_001328129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	chromatin assembly factor 1 subunit B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX571809.10 and CT737124.22. On Jun 7, 2016 this sequence version replaced XM_005167689.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01021798.1, GDQH01018889.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAAGGTTG TAACCTGTGA GATTGCCTGG CATAATAAGG AGCCAGTGTA CAGTCTGGAC 
TTTCAACAGA GCGGTGATGG AAAAACACAG CGTCTGGCCA CTGCTGGAGT GGACACCACA 
GTCCGGATGT GGCGTGTGGA TAAAGGGCCT GATGGGAAAG CGGTCGTTGA GTTTTTGTCT 
AACTTGGCAC GACACACGAA AGCAGTGAAT GTAGTGCGTT TCTCACCCAC TGCTGAAGTG 
TTGGCTTCTG GGGGAGATGA TGCGGCCATC TTGCTTTGGA AGCTGAATGA CAATAAGGAG 
CCTGAACAAA CTCCGACCTT TCAGGAAGAG GAAGATGCTC AACTTAATAA AGAGAGCTGG 
AGTGTGGTCA AGACTCTAAG GGGACATATT GAGGATGTAT ATGATATCTC ATGGACAAGT 
GATGGGAATT TCATGGCTTC AGGCTCTGTG GACAACACTG CCATCATGTG GGACGTCAAC 
AAGGGTCAGA AGATGTGCAT CTTTAATGAT CATAAGAGTT ATGTGCAAGG AGTAGCGTGG 
GACCCACTTG GACAGTACAT CAGCACATTA AGCTGTGACA GGGTGATGCG TGTGTACAGT 
GCTCACAACA GAAAAAAAGC TTACAGCATC AGTAAGATGA CATCATCCGC TACTGCAGAT 
GGAGAGGTGA AGAACTACAG AATGTTTCAT GATGACAGCA TGAGATCGTT TTTCAGACGT 
CTCACTTTCA CACCAGATGG CTCATTCCTC CTGGCGCCAG CTGGGTGTGT TGAGGCTGGA 
GAGAATGTCA CAAACACAAC ATATGTGTTC TCTAGAAAAA GCTTTAAGAG GCCTATTGCT 
CATCTGCCCT GTCCATCTAA AGCAACCCTG GCTGTCCGCT GCAGTCCGGT CTACTTTGAA 
CTGAGGACGA AGAGAGCTGA AGATGATTCT CTCAAGCCAG TGGCAAACAC ATTTAATTTG 
CCATATCGAC TGGTGTTTGC AGTTGCATCA GAAGACTCTA TCTTCTTTTA CGACACACAA 
CAGACACTCC CATTCGGATA TGTGTCCAAC ATCCACTATC ACACTTTAAG TGATCTCAGC 
TGGTCTCGTG ATGGCTCCTT CTTGGCAGTT TCATCCACTG ACGGTTACTG CTCCTTCATA 
TCATTTGATG AGGGGGAGCT CGGCACCCCA CTGAAGGAGA AACCACCACT GGAAATGGTG 
ACTCCATCTT CCACCAATGA GAAGAAAGGC AAGAGAGCCT TAGCCAATGG CAGGACTGCT 
TCCCCAGTGC CTCGCCAGGC CAATACACCT GCTGCACAGG ACAAAGAGAA AGAGGGATCT 
GCTGGTCCTG CGTCCTCCAA AACCAGCAGT GTTTCTGGTA CAGAAGAGAA ACGCACACCG 
CAGGCTCTGA AAACCAAAGC TCAGCCCAGA AGAATCACAC TCAACACTCT TGAGGGATGG 
GGCAAACCCA GCACCCCCAA ATCCCCAGCA GCCGCTTCCA GTACTGCCCC CAGCACACCC 
AAATCTGCTC CCTCCACCCC TGGGTCCAGC AGAGCACCGC TAACTCCCCA TACAGCCCCC 
AGCACTCCCC TTGGACCACT CAACTCTAAA AATGGCACAA CACCAAAAGG ACCCACTCCA 
AGACGTATTT CACTGACTCC AGTTGTTTCA AAATCCCCTG CTGCTTTCGT CCCTCCATCA 
TCCACAGAGA AAGCCAAACA TGAGAGACCG TCTCCTTCCA GTGACCCTGC ATGCAAACCT 
CCTGAATCTA AGAGATCCAA GACTGATGTC CCTGATGGTT CTGTCCAGAG CAGCAAGAAC 
AAAGAAGCCA CCGAAATTAC TGTCAAACCC AAGGAACCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315058.1
CDS	167..2008
Translation	MKVVTCEIAWHNKEPVYSLDFQQSGDGKTQRLATAGVDTTVRMWRVDKGPDGKAVVEFLSNLARHTKAVNVVRFSPTAEVLASGGDDAAILLWKLNDNKEPEQTPTFQEEEDAQLNKESWSVVKTLRGHIEDVYDISWTSDGNFMASGSVDNTAIMWDVNKGQKMCIFNDHKSYVQGVAWDPLGQYISTLSCDRVMRVYSAHNRKKAYSISKMTSSATADGEVKNYRMFHDDSMRSFFRRLTFTPDGSFLLAPAGCVEAGENVTNTTYVFSRKSFKRPIAHLPCPSKATLAVRCSPVYFELRTKRAEDDSLKPVANTFNLPYRLVFAVASEDSIFFYDTQQTLPFGYVSNIHYHTLSDLSWSRDGSFLAVSSTDGYCSFISFDEGELGTPLKEKPPLEMVTPSSTNEKKGKRALANGRTASPVPRQANTPAAQDKEKEGSAGPASSKTSSVSGTEEKRTPQALKTKAQPRRITLNTLEGWGKPSTPKSPAAASSTAPSTPKSAPSTPGSSRAPLTPHTAPSTPLGPLNSKNGTTPKGPTPRRISLTPVVSKSPAAFVPPSSTEKAKHERPSPSSDPACKPPESKRSKTDVPDGSVQSSKNKEATEITVKPKEP

Target ORF information:

RefSeq Version	NM_001328129.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chromatin assembly factor 1, subunit B (chaf1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328129.1

ORF Insert Sequence:

ATGAAGGTTG TAACCTGTGA GATTGCCTGG CATAATAAGG AGCCAGTGTA CAGTCTGGAC 
TTTCAACAGA GCGGTGATGG AAAAACACAG CGTCTGGCCA CTGCTGGAGT GGACACCACA 
GTCCGGATGT GGCGTGTGGA TAAAGGGCCT GATGGGAAAG CGGTCGTTGA GTTTTTGTCT 
AACTTGGCAC GACACACGAA AGCAGTGAAT GTAGTGCGTT TCTCACCCAC TGCTGAAGTG 
TTGGCTTCTG GGGGAGATGA TGCGGCCATC TTGCTTTGGA AGCTGAATGA CAATAAGGAG 
CCTGAACAAA CTCCGACCTT TCAGGAAGAG GAAGATGCTC AACTTAATAA AGAGAGCTGG 
AGTGTGGTCA AGACTCTAAG GGGACATATT GAGGATGTAT ATGATATCTC ATGGACAAGT 
GATGGGAATT TCATGGCTTC AGGCTCTGTG GACAACACTG CCATCATGTG GGACGTCAAC 
AAGGGTCAGA AGATGTGCAT CTTTAATGAT CATAAGAGTT ATGTGCAAGG AGTAGCGTGG 
GACCCACTTG GACAGTACAT CAGCACATTA AGCTGTGACA GGGTGATGCG TGTGTACAGT 
GCTCACAACA GAAAAAAAGC TTACAGCATC AGTAAGATGA CATCATCCGC TACTGCAGAT 
GGAGAGGTGA AGAACTACAG AATGTTTCAT GATGACAGCA TGAGATCGTT TTTCAGACGT 
CTCACTTTCA CACCAGATGG CTCATTCCTC CTGGCGCCAG CTGGGTGTGT TGAGGCTGGA 
GAGAATGTCA CAAACACAAC ATATGTGTTC TCTAGAAAAA GCTTTAAGAG GCCTATTGCT 
CATCTGCCCT GTCCATCTAA AGCAACCCTG GCTGTCCGCT GCAGTCCGGT CTACTTTGAA 
CTGAGGACGA AGAGAGCTGA AGATGATTCT CTCAAGCCAG TGGCAAACAC ATTTAATTTG 
CCATATCGAC TGGTGTTTGC AGTTGCATCA GAAGACTCTA TCTTCTTTTA CGACACACAA 
CAGACACTCC CATTCGGATA TGTGTCCAAC ATCCACTATC ACACTTTAAG TGATCTCAGC 
TGGTCTCGTG ATGGCTCCTT CTTGGCAGTT TCATCCACTG ACGGTTACTG CTCCTTCATA 
TCATTTGATG AGGGGGAGCT CGGCACCCCA CTGAAGGAGA AACCACCACT GGAAATGGTG 
ACTCCATCTT CCACCAATGA GAAGAAAGGC AAGAGAGCCT TAGCCAATGG CAGGACTGCT 
TCCCCAGTGC CTCGCCAGGC CAATACACCT GCTGCACAGG ACAAAGAGAA AGAGGGATCT 
GCTGGTCCTG CGTCCTCCAA AACCAGCAGT GTTTCTGGTA CAGAAGAGAA ACGCACACCG 
CAGGCTCTGA AAACCAAAGC TCAGCCCAGA AGAATCACAC TCAACACTCT TGAGGGATGG 
GGCAAACCCA GCACCCCCAA ATCCCCAGCA GCCGCTTCCA GTACTGCCCC CAGCACACCC 
AAATCTGCTC CCTCCACCCC TGGGTCCAGC AGAGCACCGC TAACTCCCCA TACAGCCCCC 
AGCACTCCCC TTGGACCACT CAACTCTAAA AATGGCACAA CACCAAAAGG ACCCACTCCA 
AGACGTATTT CACTGACTCC AGTTGTTTCA AAATCCCCTG CTGCTTTCGT CCCTCCATCA 
TCCACAGAGA AAGCCAAACA TGAGAGACCG TCTCCTTCCA GTGACCCTGC ATGCAAACCT 
CCTGAATCTA AGAGATCCAA GACTGATGTC CCTGATGGTT CTGTCCAGAG CAGCAAGAAC 
AAAGAAGCCA CCGAAATTAC TGTCAAACCC AAGGAACCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The phenotype of the good effort mutant zebrafish is retinal degeneration by cell death and is linked to the chromosome assembly factor 1b gene.
Advances in experimental medicine and biology801535-41(2014)
Bailey TJ,Hyde DR

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Nature cell biology15(12)1516-25(2013 Dec)
Huang HT,Kathrein KL,Barton A,Gitlin Z,Huang YH,Ward TP,Hofmann O,Dibiase A,Song A,Tyekucheva S,Hide W,Zhou Y,Zon LI

Mutation of zebrafish caf-1b results in S phase arrest, defective differentiation, and p53-mediated apoptosis during organogenesis.
Cell cycle (Georgetown, Tex.)6(23)2962-9(2007 Dec)
Fischer S,Prykhozhij S,Rau MJ,Neumann CJ