nos2a cDNA ORF clone, Danio rerio(zebrafish)

The following nos2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nos2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa00438 NM_001104937.1
Latest version!
Danio rerio nitric oxide synthase 2a, inducible (nos2a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa10688 XM_005165295.3
Latest version!
Danio rerio nitric oxide synthase 2a, inducible (nos2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa10688 XM_005165296.4
Latest version!
Danio rerio nitric oxide synthase 2a, inducible (nos2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
ODa10688 XM_005165296.3 Danio rerio nitric oxide synthase 2a, inducible (nos2a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa00438
    Clone ID Related Accession (Same CDS sequence) NM_001104937.1
    Accession Version NM_001104937.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3240bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-20
    Organism Danio rerio(zebrafish)
    Product nitric oxide synthase 2a, inducible
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AM084880.1. On Sep 16, 2007 this sequence version replaced XM_001333956.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AM084880.1, AM749801.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA708836, SAMN01765705 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGAAGAC AAGCACAAAC CACTATCATT GAAAATGAGA AACAGACTGT GCAATCAAAG 
    CGGCCAGTCG ATCGACCAAT TCGATGTCCA TTCGCTGTTC AGTTACGCAA CTACAAGGAT
    GGCTCGACCC ACCAGGACTC ACTTCATCAC AAAGCGGCTA AGAATACGCC CTGCAATTCA
    AAAACTTGTG AGGGCTCCAT TATGAATCCC AAACCTCTGA TCCGCGGCCC ATCCAAAATC
    ACACCTTCCC CAGATGACAT GCTCAGCCAG GCAGAGGATT TCATCGACCA GTACTACAAG
    TCGCTCAAAA TCCCGGGCTC GACGGAGCAG CAAGCGCGGA TTGACAGGAT CGCGCTGGAG
    ATCACTCTGA CCGGGACCTA CTCTCTCACC ACCGAGGAAC TCGCTTTTGG GGCCAAACAG
    GCGTGGAGAA ATGCGCCAAG ATGCATTGGC AGGATCCAGT GGTCCAATTT ACAGCTTTTC
    GATGCCCGTG ATTGTAAAAC CGCTGAGGAC ATGTTTCATG CCTTGTGCAC TCATCTGAAG
    TATGCCACCA ACGGAGGAAA CTTGAGGTCT GCAATCACTG TGTTCCCTCA GAGAACAGAT
    GAAGGGCATG ATTTCCGTGT TTGGAACGGT CAGCTGATAA AGTATGCTGG CTATCAGATG
    GATGATGGTA GTGTGATCGG CGACCCAGCG GGTGTAGACT TTACACAGGT GTGTATTCAG
    CTTGGGTGGA AACCAAAGTA CGGTCGCTTT GATGTGCTTC CACTAGTCCT GCAGGCGAAT
    GGAGAAGACC CTGAAGTTTT TGAAATTCCT CCTGAACTGA TCTTGGAGGT CCCCATTGAA
    CATCCAGAGT ATGAATGGTT CAAAGATCTA GACCTGAAGT GGTACGCGAT TCCTGCAGTG
    GCCAACATGC TCATGGAAGT TGGTGGTCTG GAATTCACAG CATGTCCTTT CAACGGCTGG
    TACATGGGCA CAGAAATTGG AGTTCGGGAC TTCTGTGACA GTCAACGCTA CAACATTTTG
    GAGCGCGTTG GCCAGTTAAT GGGCTTAGAA ACACGCAAGC TCTCATCACT GTGGAAAGAC
    CAGGCACTCG TGGCTATCAA TGTTGCCGTC ATTCACAGCT ATCAGAAAAA TAAGGTCACC
    ATCACAGATC ACCATTCCGC AAGCGAGTCC TTCATGCAGC ATTTGGAGAC GGAGGTTCGT
    TTGCGTGGAG GCTGCCCAGC GGATTGGGTC TGGCTGGTGC CACCGATGTC TGGTTCCCTG
    ACCCCCGTCT ATCATCAGGA GTTGCTCAAT TACATCCTGT CCCCATTCTT CTATTACCAG
    ACTGATCCAT GGCTAACACA CAAGTGGAAA GATGAGAAGA AAGGACTTCG GAGATGCAAG
    GTCAGCTTCA AAGGACTGAT CAGAGCTGTA TTTTTCTCTC AAACACTCAT CAAAGCTGTC
    CTGGCCAAGA GAGTGCGCTG TACTGTTCTC TATGCCACAG AAACAGGGAA GTCACTGAGC
    TTTGCCAAAA AACTGAACAC CATGCTCAAC CGGGCCTTTG ACTCCAGGCT GGTATGCATG
    GAGGAATATG ACTTCAGTGA ACTTGAGACA GAGACCCTTC TGATGGTGGT CACCAGTACC
    TTTGGCAATG GAGAAAGTCC AGGAAATGGA GTGACCTTCA GGAAGCAGCT TTTTAGCTTG
    CAAAAAGTGA AAAACACCAT CAGGTACGGT GTCTTTGGCC TGGGTTCACG TGTATACCCA
    CAGTTCTGTG CCTTTGCCCA TGCTGTGGAT GACAAGCTTG CGGAGCTGGG GGCCAAACGT
    ATGACAGCCA CAGGCGAGGG TGATGAACTA AACGGGCAGG ACGAGGCTTT CTCTGCCTGG
    GCTTGCACTG CTTTTAAGGA TGCTTGTGAG GAGTTTAACA TTAAAAAGCA GCTGAAAGCT
    GTGGAGGAGC AGACGGAAGG CTGGGACCCT CAGAGATACA GAGTCCAGCA GGACAGTTGC
    ACTCTGGATC CCATTACAGC ACTGTCTGCT CTTCACTCTA AAACCGTGTT TCCTATGACT
    CTGAAGAGAA GACAGAAACT GCAAAGTGCT CAGTCAAGTC GTTCTACTAT CCTGGTGGAG
    CTGAAGACCG ATGGAAACAC AGATGCCTTG AAGTTTGCCC CAGGAGATCA TGTGGGGATT
    TTTCCAGAGA ATTCTTCAGA ATTGGTGCAT GGTATCGTGA AGCATCTCCC AGATGCCACT
    CCTGATAACC AGAGCCTTCG TCTGGAGACC CTCTCTGAGT CCAGCCATGA GGGAAAGAGA
    TGGCAGACAG ACGAACGTCT CCCTGCTTGC ACGCTCGTCC AGGCTCTAAC ATACTTCCTG
    GATGTCACGA CTCCACCTTC ACAAAGCCTG CTGCGCAAAC TCTCTACAGC GGCGGATCAG
    GAGGAGGATC GCAAACGACT TGAAGCGCTC GCATCGGACT TCCAGGAATA TTCCACATGG
    AAGGATTTCT ACAGGCCCAC CTTCTTGGAG GTTCTTGAAG AATTTCCCTC TCTGAAAGTC
    CCTTCCGCCT TCATCCTGAG TCAGATTCCT CTGCTGAAGC CCCGTCTGTA CTCCGTCAGC
    TCCTCGCTGG ATCATCATCC CAATGAACTA CACCTAACAA TCTCTGTGGT GGAGTACCAG
    ACCCAGGGTG GAGCAGGCCC AATGCATTTC GGCACTTGCA GCACGTGGTT CAACACCATC
    AAAAGAGGAC ACACAGTTCC CTGCTTTGTT CACCGCTCGG GGGGTTTCCA TCTCCCAGAA
    GACCCCAGCA CTCCTGTTCT CCTGATCGGG GCTGGCAGTG GAATTGCTCC TTTCCGCAGT
    TTCTGGCAGC AGCGCACTCA GGATGTGAAA AAGACAGGTC AGCAAATTAG CCCCATGACC
    CTACTGTTTG GCTGTCGCGA TTCAGAAACA GACCACCTGT ACAAAGTAGA AACCCTGAAG
    TTGAGACTTA ATGGTATTCT GGGAAACGTC AATACAGCTT ATTCACGCCA GCCTGGGAAA
    CCCAAGATGT ACGTCCAGGA TGTTTTGAAG GAGCAGCTTG TTGAAAAGGC TTTGGAGGTT
    CTTCACAAGA GCGCAGGTCA TCTTTATGTC TGCGGTGGAA TGAACATGGC CAAAGATGTG
    GCTCAAACTT TGAAAGATAT CCTGGTCAAC AACACAATCA TGAGCGAGGT AGAAGCTGGA
    GAATATATGG ATATGTTGAA GGTTGAGAAG AGATACCATG AAGACATTTT TGGAGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001098407.1
    CDS83..3322
    Translation

    Target ORF information:

    RefSeq Version NM_001104937.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio nitric oxide synthase 2a, inducible (nos2a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001104937.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGAAGAC AAGCACAAAC CACTATCATT GAAAATGAGA AACAGACTGT GCAATCAAAG 
    CGGCCAGTCG ATCGACCAAT TCGATGTCCA TTCGCTGTTC AGTTACGCAA CTACAAGGAT
    GGCTCGACCC ACCAGGACTC ACTTCATCAC AAAGCGGCTA AGAATACGCC CTGCAATTCA
    AAAACTTGTG AGGGCTCCAT TATGAATCCC AAACCTCTGA TCCGCGGCCC ATCCAAAATC
    ACACCTTCCC CAGATGACAT GCTCAGCCAG GCAGAGGATT TCATCGACCA GTACTACAAG
    TCGCTCAAAA TCCCGGGCTC GACGGAGCAG CAAGCGCGGA TTGACAGGAT CGCGCTGGAG
    ATCACTCTGA CCGGGACCTA CTCTCTCACC ACCGAGGAAC TCGCTTTTGG GGCCAAACAG
    GCGTGGAGAA ATGCGCCAAG ATGCATTGGC AGGATCCAGT GGTCCAATTT ACAGCTTTTC
    GATGCCCGTG ATTGTAAAAC CGCTGAGGAC ATGTTTCATG CCTTGTGCAC TCATCTGAAG
    TATGCCACCA ACGGAGGAAA CTTGAGGTCT GCAATCACTG TGTTCCCTCA GAGAACAGAT
    GAAGGGCATG ATTTCCGTGT TTGGAACGGT CAGCTGATAA AGTATGCTGG CTATCAGATG
    GATGATGGTA GTGTGATCGG CGACCCAGCG GGTGTAGACT TTACACAGGT GTGTATTCAG
    CTTGGGTGGA AACCAAAGTA CGGTCGCTTT GATGTGCTTC CACTAGTCCT GCAGGCGAAT
    GGAGAAGACC CTGAAGTTTT TGAAATTCCT CCTGAACTGA TCTTGGAGGT CCCCATTGAA
    CATCCAGAGT ATGAATGGTT CAAAGATCTA GACCTGAAGT GGTACGCGAT TCCTGCAGTG
    GCCAACATGC TCATGGAAGT TGGTGGTCTG GAATTCACAG CATGTCCTTT CAACGGCTGG
    TACATGGGCA CAGAAATTGG AGTTCGGGAC TTCTGTGACA GTCAACGCTA CAACATTTTG
    GAGCGCGTTG GCCAGTTAAT GGGCTTAGAA ACACGCAAGC TCTCATCACT GTGGAAAGAC
    CAGGCACTCG TGGCTATCAA TGTTGCCGTC ATTCACAGCT ATCAGAAAAA TAAGGTCACC
    ATCACAGATC ACCATTCCGC AAGCGAGTCC TTCATGCAGC ATTTGGAGAC GGAGGTTCGT
    TTGCGTGGAG GCTGCCCAGC GGATTGGGTC TGGCTGGTGC CACCGATGTC TGGTTCCCTG
    ACCCCCGTCT ATCATCAGGA GTTGCTCAAT TACATCCTGT CCCCATTCTT CTATTACCAG
    ACTGATCCAT GGCTAACACA CAAGTGGAAA GATGAGAAGA AAGGACTTCG GAGATGCAAG
    GTCAGCTTCA AAGGACTGAT CAGAGCTGTA TTTTTCTCTC AAACACTCAT CAAAGCTGTC
    CTGGCCAAGA GAGTGCGCTG TACTGTTCTC TATGCCACAG AAACAGGGAA GTCACTGAGC
    TTTGCCAAAA AACTGAACAC CATGCTCAAC CGGGCCTTTG ACTCCAGGCT GGTATGCATG
    GAGGAATATG ACTTCAGTGA ACTTGAGACA GAGACCCTTC TGATGGTGGT CACCAGTACC
    TTTGGCAATG GAGAAAGTCC AGGAAATGGA GTGACCTTCA GGAAGCAGCT TTTTAGCTTG
    CAAAAAGTGA AAAACACCAT CAGGTACGGT GTCTTTGGCC TGGGTTCACG TGTATACCCA
    CAGTTCTGTG CCTTTGCCCA TGCTGTGGAT GACAAGCTTG CGGAGCTGGG GGCCAAACGT
    ATGACAGCCA CAGGCGAGGG TGATGAACTA AACGGGCAGG ACGAGGCTTT CTCTGCCTGG
    GCTTGCACTG CTTTTAAGGA TGCTTGTGAG GAGTTTAACA TTAAAAAGCA GCTGAAAGCT
    GTGGAGGAGC AGACGGAAGG CTGGGACCCT CAGAGATACA GAGTCCAGCA GGACAGTTGC
    ACTCTGGATC CCATTACAGC ACTGTCTGCT CTTCACTCTA AAACCGTGTT TCCTATGACT
    CTGAAGAGAA GACAGAAACT GCAAAGTGCT CAGTCAAGTC GTTCTACTAT CCTGGTGGAG
    CTGAAGACCG ATGGAAACAC AGATGCCTTG AAGTTTGCCC CAGGAGATCA TGTGGGGATT
    TTTCCAGAGA ATTCTTCAGA ATTGGTGCAT GGTATCGTGA AGCATCTCCC AGATGCCACT
    CCTGATAACC AGAGCCTTCG TCTGGAGACC CTCTCTGAGT CCAGCCATGA GGGAAAGAGA
    TGGCAGACAG ACGAACGTCT CCCTGCTTGC ACGCTCGTCC AGGCTCTAAC ATACTTCCTG
    GATGTCACGA CTCCACCTTC ACAAAGCCTG CTGCGCAAAC TCTCTACAGC GGCGGATCAG
    GAGGAGGATC GCAAACGACT TGAAGCGCTC GCATCGGACT TCCAGGAATA TTCCACATGG
    AAGGATTTCT ACAGGCCCAC CTTCTTGGAG GTTCTTGAAG AATTTCCCTC TCTGAAAGTC
    CCTTCCGCCT TCATCCTGAG TCAGATTCCT CTGCTGAAGC CCCGTCTGTA CTCCGTCAGC
    TCCTCGCTGG ATCATCATCC CAATGAACTA CACCTAACAA TCTCTGTGGT GGAGTACCAG
    ACCCAGGGTG GAGCAGGCCC AATGCATTTC GGCACTTGCA GCACGTGGTT CAACACCATC
    AAAAGAGGAC ACACAGTTCC CTGCTTTGTT CACCGCTCGG GGGGTTTCCA TCTCCCAGAA
    GACCCCAGCA CTCCTGTTCT CCTGATCGGG GCTGGCAGTG GAATTGCTCC TTTCCGCAGT
    TTCTGGCAGC AGCGCACTCA GGATGTGAAA AAGACAGGTC AGCAAATTAG CCCCATGACC
    CTACTGTTTG GCTGTCGCGA TTCAGAAACA GACCACCTGT ACAAAGTAGA AACCCTGAAG
    TTGAGACTTA ATGGTATTCT GGGAAACGTC AATACAGCTT ATTCACGCCA GCCTGGGAAA
    CCCAAGATGT ACGTCCAGGA TGTTTTGAAG GAGCAGCTTG TTGAAAAGGC TTTGGAGGTT
    CTTCACAAGA GCGCAGGTCA TCTTTATGTC TGCGGTGGAA TGAACATGGC CAAAGATGTG
    GCTCAAACTT TGAAAGATAT CCTGGTCAAC AACACAATCA TGAGCGAGGT AGAAGCTGGA
    GAATATATGG ATATGTTGAA GGTTGAGAAG AGATACCATG AAGACATTTT TGGAGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa10688
    Clone ID Related Accession (Same CDS sequence) XM_005165295.3 , XM_005165296.3 , XM_005165296.4
    Accession Version XM_005165295.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3240bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product nitric oxide synthase 2a, inducible isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165295.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGAAGAC AAGCACAAAC CACTATCATT GAAAATGAGA AACAGACTGT GCAATCAAAG 
    CGGCCAGTCG ATCGACCAAT TCGATGTCCA TTCGCTGTTC AGTTACGCAA CTACAAGGAT
    GGCTCGACCC ACCAGGACTC ACTTCATCAC AAAGCGGCTA AGAATACGCC CTGCAATTCA
    AAAACTTGTG AGGGCTCCAT TATGAATCCC AAACCTCTGA TCCGCGGCCC ATCCAAAATC
    ACACCTTCCC CAGATGACAT GCTCAGCCAG GCAGAGGATT TCATCGACCA GTACTACAAG
    TCGCTCAAAA TCCCGGGCTC GACGGAGCAG CAAGCGCGGA TTGACAGGAT CGCGCTGGAG
    ATCACTCTGA CCGGGACCTA CTCTCTCACC ACCGAGGAAC TCGCTTTTGG GGCCAAACAG
    GCGTGGAGAA ATGCGCCAAG ATGCATTGGC AGGATCCAGT GGTCCAATTT ACAGCTTTTC
    GATGCCCGTG ATTGTAAAAC CGCTGAGGAC ATGTTTCATG CCTTGTGCAC TCATCTTAAG
    TATGCCACCA ACGGAGGAAA CTTGAGGTCT GCCATCACCG TGTTCCCTCA GAGAACAGAT
    GAAGGGCATG ATTTCCGTGT TTGGAACGGT CAGCTGATAA AGTATGCTGG CTATCAGATG
    GATGATGGTA GTGTGATCGG CGACCCAGCG GGTGTAGACT TTACACAGGT GTGTATTCAG
    CTTGGGTGGA AACCAAAGTA CGGTCGCTTT GATGTGCTTC CACTAGTCCT GCAGGCGAAT
    GGAGAAGACC CTGAAGTTTT TGAAATTCCT CCTGAACTGA TCTTGGAGGT CCCCATTGAA
    CATCCAGAGT ATGAATGGTT CAAAGATCTA GACCTGAAGT GGTACGCGAT TCCTGCAGTG
    GCCAACATGC TCATGGAAGT TGGTGGTCTG GAATTCACAG CATGTCCTTT CAACGGCTGG
    TACATGGGCA CAGAAATTGG AGTTCGGGAC TTCTGTGACA GTCAACGCTA CAACATTTTG
    GAGCGCGTTG GCCAGTTAAT GGGCTTAGAA ACACGCAAGC TCTCATCACT GTGGAAAGAC
    CAGGCACTCG TGGCTATCAA TGTTGCCGTC ATTCACAGCT ATCAGAAAAA TAAGGTCACC
    ATCACAGATC ACCATTCCGC AAGCGAGTCC TTCATGCAGC ATTTGGAGAC GGAGGTTCGT
    TTGCGTGGAG GCTGCCCAGC GGATTGGGTC TGGCTGGTGC CACCGATGTC TGGTTCCCTG
    ACCCCCGTCT ATCATCAGGA GTTGCTCAAT TACATCCTGT CCCCATTCTT CTATTACCAG
    ACTGATCCAT GGCTAACACA CAAGTGGAAA GATGAGAAGA AAGGACTTCG GAGATGCAAG
    GTCAGCTTCA AAGGACTGAT CAGAGCTGTA TTTTTCTCTC AAACACTCAT CAAAGCTGTC
    CTGGCCAAGA GAGTGCGCTG TACTGTTCTC TATGCCACAG AAACAGGGAA GTCACTGAGC
    TTTGCCAAAA AACTGAACAC CATGCTCAAC CGGGCCTTTG ACTCCAGGCT GGTATGCATG
    GAGGAATATG ACTTCAGTGA ACTTGAGACA GAGACCCTTC TGATGGTGGT CACCAGTACC
    TTTGGCAATG GAGAAAGTCC AGGAAATGGA GTGACCTTCA GGAAGCAGCT TTTTAGCTTG
    CAAAAAGTGA AAAACACCAT CAGGTACGGT GTCTTTGGCC TGGGTTCACG TGTATACCCA
    CAGTTCTGTG CCTTTGCCCA TGCTGTGGAT GACAAGCTTG CGGAGCTGGG GGCCAAACGT
    ATGACAGCCA CAGGCGAGGG TGATGAACTA AACGGGCAGG ACGAGGCTTT CTCTGCCTGG
    GCTTGCACTG CTTTTAAGGA TGCTTGTGAG GAGTTTAACA TTAAAAAGCA GCTGAAAGCT
    GTGGAGGAGC AGACGGAAGG CTGGGACCCT CAGAGATACA GAGTCCAGCA GGACAGTTGC
    ACTCTGGATC CCATTACAGC ACTGTCTGCT CTTCACTCTA AAACCGTGTT TCCTATGACT
    CTGAAGAGAA GACAGAAACT GCAAAGTGCT CAGTCAAGTC GTTCTACTAT CCTGGTGGAG
    CTGAAGACCG ATGGAAACAC AGATGCCTTG AAGTTTGCCC CAGGAGATCA TGTGGGGATT
    TTTCCAGAGA ATTCTTCAGA ATTGGTGCAT GGTATCGTGA AGCATCTCCC AGATGCCACT
    CCTGATAACC AGAGCCTTCG TCTGGAGACC CTCTCTGAGT CCAGCCATGA GGGAAAGAGA
    TGGCAGACAG ACGAACGTCT CCCTGCTTGC ACGCTCGTCC AGGCTCTAAC ATACTTCCTG
    GATGTCACGA CTCCACCTTC ACAAAGCCTG CTGCGCAAAC TCTCTACAGT GGCGGATCAG
    GAAGAGGATC GCAAACGACT TGAAGCGCTC GCATCGGACT TCCAGGAATA TTCCACATGG
    AAGGATTTCT ACAGGCCCAC CTTCTTGGAG GTTCTTGAAG AATTTCCCTC TCTGAAAGTC
    CCTTCCGCCT TCATCCTGAG TCAGATTCCT CTGCTGAAGC CCCGTCTGTA CTCCGTCAGC
    TCCTCGCTGG ATCATCATCC CAATGAACTA CACCTAACAA TCTCTGTGGT GGAGTACCAG
    ACCCAGGGTG GAGCAGGCCC AATGCATTTC GGCACTTGCA GCACGTGGTT CAACACCATC
    AAAAGAGGAC ACACAGTTCC CTGCTTTGTT CACCGCTCGG GGGGTTTCCA TCTCCCAGAA
    GACCCCAGCA CTCCTGTTCT CCTGATCGGG GCTGGCAGTG GAATTGCTCC TTTCCGCAGT
    TTCTGGCAGC AGCGCACTCA GGATGTGAAA AAGACAGGTC AGCAAATTAG CCCCATGACC
    CTACTGTTTG GCTGTCGCGA TTCAGAAACA GACCACCTGT ACAAAGTAGA AACCCTGAAG
    TTGAGACTTA ATGGTATTCT GGGAAACGTC AATACAGCTT ATTCACGCCA GCCTGGGAAA
    CCCAAGATGT ACGTCCAGGA TGTTTTGAAG GAGCAGCTTG TTGAAAAGGC TTTGGAGGTT
    CTTCACAAGA GCGCAGGTCA TCTTTATGTC TGCGGTGGAA TGAACATGGC CAAAGATGTG
    GCTCAAACTT TGAAAGATAT CCTGGTCAAC AACACAATCA TGAGCGAGGT AGAAGCTGGA
    GAATATATGG ATATGTTGAA GGTTGAGAAG AGATACCATG AAGACATTTT TGGAGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005165352.1
    CDS255..3494
    Translation

    Target ORF information:

    RefSeq Version XM_005165295.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio nitric oxide synthase 2a, inducible (nos2a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005165295.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGAAGAC AAGCACAAAC CACTATCATT GAAAATGAGA AACAGACTGT GCAATCAAAG 
    CGGCCAGTCG ATCGACCAAT TCGATGTCCA TTCGCTGTTC AGTTACGCAA CTACAAGGAT
    GGCTCGACCC ACCAGGACTC ACTTCATCAC AAAGCGGCTA AGAATACGCC CTGCAATTCA
    AAAACTTGTG AGGGCTCCAT TATGAATCCC AAACCTCTGA TCCGCGGCCC ATCCAAAATC
    ACACCTTCCC CAGATGACAT GCTCAGCCAG GCAGAGGATT TCATCGACCA GTACTACAAG
    TCGCTCAAAA TCCCGGGCTC GACGGAGCAG CAAGCGCGGA TTGACAGGAT CGCGCTGGAG
    ATCACTCTGA CCGGGACCTA CTCTCTCACC ACCGAGGAAC TCGCTTTTGG GGCCAAACAG
    GCGTGGAGAA ATGCGCCAAG ATGCATTGGC AGGATCCAGT GGTCCAATTT ACAGCTTTTC
    GATGCCCGTG ATTGTAAAAC CGCTGAGGAC ATGTTTCATG CCTTGTGCAC TCATCTTAAG
    TATGCCACCA ACGGAGGAAA CTTGAGGTCT GCCATCACCG TGTTCCCTCA GAGAACAGAT
    GAAGGGCATG ATTTCCGTGT TTGGAACGGT CAGCTGATAA AGTATGCTGG CTATCAGATG
    GATGATGGTA GTGTGATCGG CGACCCAGCG GGTGTAGACT TTACACAGGT GTGTATTCAG
    CTTGGGTGGA AACCAAAGTA CGGTCGCTTT GATGTGCTTC CACTAGTCCT GCAGGCGAAT
    GGAGAAGACC CTGAAGTTTT TGAAATTCCT CCTGAACTGA TCTTGGAGGT CCCCATTGAA
    CATCCAGAGT ATGAATGGTT CAAAGATCTA GACCTGAAGT GGTACGCGAT TCCTGCAGTG
    GCCAACATGC TCATGGAAGT TGGTGGTCTG GAATTCACAG CATGTCCTTT CAACGGCTGG
    TACATGGGCA CAGAAATTGG AGTTCGGGAC TTCTGTGACA GTCAACGCTA CAACATTTTG
    GAGCGCGTTG GCCAGTTAAT GGGCTTAGAA ACACGCAAGC TCTCATCACT GTGGAAAGAC
    CAGGCACTCG TGGCTATCAA TGTTGCCGTC ATTCACAGCT ATCAGAAAAA TAAGGTCACC
    ATCACAGATC ACCATTCCGC AAGCGAGTCC TTCATGCAGC ATTTGGAGAC GGAGGTTCGT
    TTGCGTGGAG GCTGCCCAGC GGATTGGGTC TGGCTGGTGC CACCGATGTC TGGTTCCCTG
    ACCCCCGTCT ATCATCAGGA GTTGCTCAAT TACATCCTGT CCCCATTCTT CTATTACCAG
    ACTGATCCAT GGCTAACACA CAAGTGGAAA GATGAGAAGA AAGGACTTCG GAGATGCAAG
    GTCAGCTTCA AAGGACTGAT CAGAGCTGTA TTTTTCTCTC AAACACTCAT CAAAGCTGTC
    CTGGCCAAGA GAGTGCGCTG TACTGTTCTC TATGCCACAG AAACAGGGAA GTCACTGAGC
    TTTGCCAAAA AACTGAACAC CATGCTCAAC CGGGCCTTTG ACTCCAGGCT GGTATGCATG
    GAGGAATATG ACTTCAGTGA ACTTGAGACA GAGACCCTTC TGATGGTGGT CACCAGTACC
    TTTGGCAATG GAGAAAGTCC AGGAAATGGA GTGACCTTCA GGAAGCAGCT TTTTAGCTTG
    CAAAAAGTGA AAAACACCAT CAGGTACGGT GTCTTTGGCC TGGGTTCACG TGTATACCCA
    CAGTTCTGTG CCTTTGCCCA TGCTGTGGAT GACAAGCTTG CGGAGCTGGG GGCCAAACGT
    ATGACAGCCA CAGGCGAGGG TGATGAACTA AACGGGCAGG ACGAGGCTTT CTCTGCCTGG
    GCTTGCACTG CTTTTAAGGA TGCTTGTGAG GAGTTTAACA TTAAAAAGCA GCTGAAAGCT
    GTGGAGGAGC AGACGGAAGG CTGGGACCCT CAGAGATACA GAGTCCAGCA GGACAGTTGC
    ACTCTGGATC CCATTACAGC ACTGTCTGCT CTTCACTCTA AAACCGTGTT TCCTATGACT
    CTGAAGAGAA GACAGAAACT GCAAAGTGCT CAGTCAAGTC GTTCTACTAT CCTGGTGGAG
    CTGAAGACCG ATGGAAACAC AGATGCCTTG AAGTTTGCCC CAGGAGATCA TGTGGGGATT
    TTTCCAGAGA ATTCTTCAGA ATTGGTGCAT GGTATCGTGA AGCATCTCCC AGATGCCACT
    CCTGATAACC AGAGCCTTCG TCTGGAGACC CTCTCTGAGT CCAGCCATGA GGGAAAGAGA
    TGGCAGACAG ACGAACGTCT CCCTGCTTGC ACGCTCGTCC AGGCTCTAAC ATACTTCCTG
    GATGTCACGA CTCCACCTTC ACAAAGCCTG CTGCGCAAAC TCTCTACAGT GGCGGATCAG
    GAAGAGGATC GCAAACGACT TGAAGCGCTC GCATCGGACT TCCAGGAATA TTCCACATGG
    AAGGATTTCT ACAGGCCCAC CTTCTTGGAG GTTCTTGAAG AATTTCCCTC TCTGAAAGTC
    CCTTCCGCCT TCATCCTGAG TCAGATTCCT CTGCTGAAGC CCCGTCTGTA CTCCGTCAGC
    TCCTCGCTGG ATCATCATCC CAATGAACTA CACCTAACAA TCTCTGTGGT GGAGTACCAG
    ACCCAGGGTG GAGCAGGCCC AATGCATTTC GGCACTTGCA GCACGTGGTT CAACACCATC
    AAAAGAGGAC ACACAGTTCC CTGCTTTGTT CACCGCTCGG GGGGTTTCCA TCTCCCAGAA
    GACCCCAGCA CTCCTGTTCT CCTGATCGGG GCTGGCAGTG GAATTGCTCC TTTCCGCAGT
    TTCTGGCAGC AGCGCACTCA GGATGTGAAA AAGACAGGTC AGCAAATTAG CCCCATGACC
    CTACTGTTTG GCTGTCGCGA TTCAGAAACA GACCACCTGT ACAAAGTAGA AACCCTGAAG
    TTGAGACTTA ATGGTATTCT GGGAAACGTC AATACAGCTT ATTCACGCCA GCCTGGGAAA
    CCCAAGATGT ACGTCCAGGA TGTTTTGAAG GAGCAGCTTG TTGAAAAGGC TTTGGAGGTT
    CTTCACAAGA GCGCAGGTCA TCTTTATGTC TGCGGTGGAA TGAACATGGC CAAAGATGTG
    GCTCAAACTT TGAAAGATAT CCTGGTCAAC AACACAATCA TGAGCGAGGT AGAAGCTGGA
    GAATATATGG ATATGTTGAA GGTTGAGAAG AGATACCATG AAGACATTTT TGGAGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa10688
    Clone ID Related Accession (Same CDS sequence) XM_005165295.3 , XM_005165296.3 , XM_005165296.4
    Accession Version XM_005165296.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3240bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product nitric oxide synthase 2a, inducible isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165296.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGAAGAC AAGCACAAAC CACTATCATT GAAAATGAGA AACAGACTGT GCAATCAAAG 
    CGGCCAGTCG ATCGACCAAT TCGATGTCCA TTCGCTGTTC AGTTACGCAA CTACAAGGAT
    GGCTCGACCC ACCAGGACTC ACTTCATCAC AAAGCGGCTA AGAATACGCC CTGCAATTCA
    AAAACTTGTG AGGGCTCCAT TATGAATCCC AAACCTCTGA TCCGCGGCCC ATCCAAAATC
    ACACCTTCCC CAGATGACAT GCTCAGCCAG GCAGAGGATT TCATCGACCA GTACTACAAG
    TCGCTCAAAA TCCCGGGCTC GACGGAGCAG CAAGCGCGGA TTGACAGGAT CGCGCTGGAG
    ATCACTCTGA CCGGGACCTA CTCTCTCACC ACCGAGGAAC TCGCTTTTGG GGCCAAACAG
    GCGTGGAGAA ATGCGCCAAG ATGCATTGGC AGGATCCAGT GGTCCAATTT ACAGCTTTTC
    GATGCCCGTG ATTGTAAAAC CGCTGAGGAC ATGTTTCATG CCTTGTGCAC TCATCTTAAG
    TATGCCACCA ACGGAGGAAA CTTGAGGTCT GCCATCACCG TGTTCCCTCA GAGAACAGAT
    GAAGGGCATG ATTTCCGTGT TTGGAACGGT CAGCTGATAA AGTATGCTGG CTATCAGATG
    GATGATGGTA GTGTGATCGG CGACCCAGCG GGTGTAGACT TTACACAGGT GTGTATTCAG
    CTTGGGTGGA AACCAAAGTA CGGTCGCTTT GATGTGCTTC CACTAGTCCT GCAGGCGAAT
    GGAGAAGACC CTGAAGTTTT TGAAATTCCT CCTGAACTGA TCTTGGAGGT CCCCATTGAA
    CATCCAGAGT ATGAATGGTT CAAAGATCTA GACCTGAAGT GGTACGCGAT TCCTGCAGTG
    GCCAACATGC TCATGGAAGT TGGTGGTCTG GAATTCACAG CATGTCCTTT CAACGGCTGG
    TACATGGGCA CAGAAATTGG AGTTCGGGAC TTCTGTGACA GTCAACGCTA CAACATTTTG
    GAGCGCGTTG GCCAGTTAAT GGGCTTAGAA ACACGCAAGC TCTCATCACT GTGGAAAGAC
    CAGGCACTCG TGGCTATCAA TGTTGCCGTC ATTCACAGCT ATCAGAAAAA TAAGGTCACC
    ATCACAGATC ACCATTCCGC AAGCGAGTCC TTCATGCAGC ATTTGGAGAC GGAGGTTCGT
    TTGCGTGGAG GCTGCCCAGC GGATTGGGTC TGGCTGGTGC CACCGATGTC TGGTTCCCTG
    ACCCCCGTCT ATCATCAGGA GTTGCTCAAT TACATCCTGT CCCCATTCTT CTATTACCAG
    ACTGATCCAT GGCTAACACA CAAGTGGAAA GATGAGAAGA AAGGACTTCG GAGATGCAAG
    GTCAGCTTCA AAGGACTGAT CAGAGCTGTA TTTTTCTCTC AAACACTCAT CAAAGCTGTC
    CTGGCCAAGA GAGTGCGCTG TACTGTTCTC TATGCCACAG AAACAGGGAA GTCACTGAGC
    TTTGCCAAAA AACTGAACAC CATGCTCAAC CGGGCCTTTG ACTCCAGGCT GGTATGCATG
    GAGGAATATG ACTTCAGTGA ACTTGAGACA GAGACCCTTC TGATGGTGGT CACCAGTACC
    TTTGGCAATG GAGAAAGTCC AGGAAATGGA GTGACCTTCA GGAAGCAGCT TTTTAGCTTG
    CAAAAAGTGA AAAACACCAT CAGGTACGGT GTCTTTGGCC TGGGTTCACG TGTATACCCA
    CAGTTCTGTG CCTTTGCCCA TGCTGTGGAT GACAAGCTTG CGGAGCTGGG GGCCAAACGT
    ATGACAGCCA CAGGCGAGGG TGATGAACTA AACGGGCAGG ACGAGGCTTT CTCTGCCTGG
    GCTTGCACTG CTTTTAAGGA TGCTTGTGAG GAGTTTAACA TTAAAAAGCA GCTGAAAGCT
    GTGGAGGAGC AGACGGAAGG CTGGGACCCT CAGAGATACA GAGTCCAGCA GGACAGTTGC
    ACTCTGGATC CCATTACAGC ACTGTCTGCT CTTCACTCTA AAACCGTGTT TCCTATGACT
    CTGAAGAGAA GACAGAAACT GCAAAGTGCT CAGTCAAGTC GTTCTACTAT CCTGGTGGAG
    CTGAAGACCG ATGGAAACAC AGATGCCTTG AAGTTTGCCC CAGGAGATCA TGTGGGGATT
    TTTCCAGAGA ATTCTTCAGA ATTGGTGCAT GGTATCGTGA AGCATCTCCC AGATGCCACT
    CCTGATAACC AGAGCCTTCG TCTGGAGACC CTCTCTGAGT CCAGCCATGA GGGAAAGAGA
    TGGCAGACAG ACGAACGTCT CCCTGCTTGC ACGCTCGTCC AGGCTCTAAC ATACTTCCTG
    GATGTCACGA CTCCACCTTC ACAAAGCCTG CTGCGCAAAC TCTCTACAGT GGCGGATCAG
    GAAGAGGATC GCAAACGACT TGAAGCGCTC GCATCGGACT TCCAGGAATA TTCCACATGG
    AAGGATTTCT ACAGGCCCAC CTTCTTGGAG GTTCTTGAAG AATTTCCCTC TCTGAAAGTC
    CCTTCCGCCT TCATCCTGAG TCAGATTCCT CTGCTGAAGC CCCGTCTGTA CTCCGTCAGC
    TCCTCGCTGG ATCATCATCC CAATGAACTA CACCTAACAA TCTCTGTGGT GGAGTACCAG
    ACCCAGGGTG GAGCAGGCCC AATGCATTTC GGCACTTGCA GCACGTGGTT CAACACCATC
    AAAAGAGGAC ACACAGTTCC CTGCTTTGTT CACCGCTCGG GGGGTTTCCA TCTCCCAGAA
    GACCCCAGCA CTCCTGTTCT CCTGATCGGG GCTGGCAGTG GAATTGCTCC TTTCCGCAGT
    TTCTGGCAGC AGCGCACTCA GGATGTGAAA AAGACAGGTC AGCAAATTAG CCCCATGACC
    CTACTGTTTG GCTGTCGCGA TTCAGAAACA GACCACCTGT ACAAAGTAGA AACCCTGAAG
    TTGAGACTTA ATGGTATTCT GGGAAACGTC AATACAGCTT ATTCACGCCA GCCTGGGAAA
    CCCAAGATGT ACGTCCAGGA TGTTTTGAAG GAGCAGCTTG TTGAAAAGGC TTTGGAGGTT
    CTTCACAAGA GCGCAGGTCA TCTTTATGTC TGCGGTGGAA TGAACATGGC CAAAGATGTG
    GCTCAAACTT TGAAAGATAT CCTGGTCAAC AACACAATCA TGAGCGAGGT AGAAGCTGGA
    GAATATATGG ATATGTTGAA GGTTGAGAAG AGATACCATG AAGACATTTT TGGAGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005165353.1
    CDS169..3408
    Translation

    Target ORF information:

    RefSeq Version XM_005165296.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio nitric oxide synthase 2a, inducible (nos2a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005165296.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGAAGAC AAGCACAAAC CACTATCATT GAAAATGAGA AACAGACTGT GCAATCAAAG 
    CGGCCAGTCG ATCGACCAAT TCGATGTCCA TTCGCTGTTC AGTTACGCAA CTACAAGGAT
    GGCTCGACCC ACCAGGACTC ACTTCATCAC AAAGCGGCTA AGAATACGCC CTGCAATTCA
    AAAACTTGTG AGGGCTCCAT TATGAATCCC AAACCTCTGA TCCGCGGCCC ATCCAAAATC
    ACACCTTCCC CAGATGACAT GCTCAGCCAG GCAGAGGATT TCATCGACCA GTACTACAAG
    TCGCTCAAAA TCCCGGGCTC GACGGAGCAG CAAGCGCGGA TTGACAGGAT CGCGCTGGAG
    ATCACTCTGA CCGGGACCTA CTCTCTCACC ACCGAGGAAC TCGCTTTTGG GGCCAAACAG
    GCGTGGAGAA ATGCGCCAAG ATGCATTGGC AGGATCCAGT GGTCCAATTT ACAGCTTTTC
    GATGCCCGTG ATTGTAAAAC CGCTGAGGAC ATGTTTCATG CCTTGTGCAC TCATCTTAAG
    TATGCCACCA ACGGAGGAAA CTTGAGGTCT GCCATCACCG TGTTCCCTCA GAGAACAGAT
    GAAGGGCATG ATTTCCGTGT TTGGAACGGT CAGCTGATAA AGTATGCTGG CTATCAGATG
    GATGATGGTA GTGTGATCGG CGACCCAGCG GGTGTAGACT TTACACAGGT GTGTATTCAG
    CTTGGGTGGA AACCAAAGTA CGGTCGCTTT GATGTGCTTC CACTAGTCCT GCAGGCGAAT
    GGAGAAGACC CTGAAGTTTT TGAAATTCCT CCTGAACTGA TCTTGGAGGT CCCCATTGAA
    CATCCAGAGT ATGAATGGTT CAAAGATCTA GACCTGAAGT GGTACGCGAT TCCTGCAGTG
    GCCAACATGC TCATGGAAGT TGGTGGTCTG GAATTCACAG CATGTCCTTT CAACGGCTGG
    TACATGGGCA CAGAAATTGG AGTTCGGGAC TTCTGTGACA GTCAACGCTA CAACATTTTG
    GAGCGCGTTG GCCAGTTAAT GGGCTTAGAA ACACGCAAGC TCTCATCACT GTGGAAAGAC
    CAGGCACTCG TGGCTATCAA TGTTGCCGTC ATTCACAGCT ATCAGAAAAA TAAGGTCACC
    ATCACAGATC ACCATTCCGC AAGCGAGTCC TTCATGCAGC ATTTGGAGAC GGAGGTTCGT
    TTGCGTGGAG GCTGCCCAGC GGATTGGGTC TGGCTGGTGC CACCGATGTC TGGTTCCCTG
    ACCCCCGTCT ATCATCAGGA GTTGCTCAAT TACATCCTGT CCCCATTCTT CTATTACCAG
    ACTGATCCAT GGCTAACACA CAAGTGGAAA GATGAGAAGA AAGGACTTCG GAGATGCAAG
    GTCAGCTTCA AAGGACTGAT CAGAGCTGTA TTTTTCTCTC AAACACTCAT CAAAGCTGTC
    CTGGCCAAGA GAGTGCGCTG TACTGTTCTC TATGCCACAG AAACAGGGAA GTCACTGAGC
    TTTGCCAAAA AACTGAACAC CATGCTCAAC CGGGCCTTTG ACTCCAGGCT GGTATGCATG
    GAGGAATATG ACTTCAGTGA ACTTGAGACA GAGACCCTTC TGATGGTGGT CACCAGTACC
    TTTGGCAATG GAGAAAGTCC AGGAAATGGA GTGACCTTCA GGAAGCAGCT TTTTAGCTTG
    CAAAAAGTGA AAAACACCAT CAGGTACGGT GTCTTTGGCC TGGGTTCACG TGTATACCCA
    CAGTTCTGTG CCTTTGCCCA TGCTGTGGAT GACAAGCTTG CGGAGCTGGG GGCCAAACGT
    ATGACAGCCA CAGGCGAGGG TGATGAACTA AACGGGCAGG ACGAGGCTTT CTCTGCCTGG
    GCTTGCACTG CTTTTAAGGA TGCTTGTGAG GAGTTTAACA TTAAAAAGCA GCTGAAAGCT
    GTGGAGGAGC AGACGGAAGG CTGGGACCCT CAGAGATACA GAGTCCAGCA GGACAGTTGC
    ACTCTGGATC CCATTACAGC ACTGTCTGCT CTTCACTCTA AAACCGTGTT TCCTATGACT
    CTGAAGAGAA GACAGAAACT GCAAAGTGCT CAGTCAAGTC GTTCTACTAT CCTGGTGGAG
    CTGAAGACCG ATGGAAACAC AGATGCCTTG AAGTTTGCCC CAGGAGATCA TGTGGGGATT
    TTTCCAGAGA ATTCTTCAGA ATTGGTGCAT GGTATCGTGA AGCATCTCCC AGATGCCACT
    CCTGATAACC AGAGCCTTCG TCTGGAGACC CTCTCTGAGT CCAGCCATGA GGGAAAGAGA
    TGGCAGACAG ACGAACGTCT CCCTGCTTGC ACGCTCGTCC AGGCTCTAAC ATACTTCCTG
    GATGTCACGA CTCCACCTTC ACAAAGCCTG CTGCGCAAAC TCTCTACAGT GGCGGATCAG
    GAAGAGGATC GCAAACGACT TGAAGCGCTC GCATCGGACT TCCAGGAATA TTCCACATGG
    AAGGATTTCT ACAGGCCCAC CTTCTTGGAG GTTCTTGAAG AATTTCCCTC TCTGAAAGTC
    CCTTCCGCCT TCATCCTGAG TCAGATTCCT CTGCTGAAGC CCCGTCTGTA CTCCGTCAGC
    TCCTCGCTGG ATCATCATCC CAATGAACTA CACCTAACAA TCTCTGTGGT GGAGTACCAG
    ACCCAGGGTG GAGCAGGCCC AATGCATTTC GGCACTTGCA GCACGTGGTT CAACACCATC
    AAAAGAGGAC ACACAGTTCC CTGCTTTGTT CACCGCTCGG GGGGTTTCCA TCTCCCAGAA
    GACCCCAGCA CTCCTGTTCT CCTGATCGGG GCTGGCAGTG GAATTGCTCC TTTCCGCAGT
    TTCTGGCAGC AGCGCACTCA GGATGTGAAA AAGACAGGTC AGCAAATTAG CCCCATGACC
    CTACTGTTTG GCTGTCGCGA TTCAGAAACA GACCACCTGT ACAAAGTAGA AACCCTGAAG
    TTGAGACTTA ATGGTATTCT GGGAAACGTC AATACAGCTT ATTCACGCCA GCCTGGGAAA
    CCCAAGATGT ACGTCCAGGA TGTTTTGAAG GAGCAGCTTG TTGAAAAGGC TTTGGAGGTT
    CTTCACAAGA GCGCAGGTCA TCTTTATGTC TGCGGTGGAA TGAACATGGC CAAAGATGTG
    GCTCAAACTT TGAAAGATAT CCTGGTCAAC AACACAATCA TGAGCGAGGT AGAAGCTGGA
    GAATATATGG ATATGTTGAA GGTTGAGAAG AGATACCATG AAGACATTTT TGGAGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa10688
    Clone ID Related Accession (Same CDS sequence) XM_005165295.3 , XM_005165296.3 , XM_005165296.4
    Accession Version XM_005165296.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3240bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product nitric oxide synthase 2a, inducible isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165296.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGAAGAC AAGCACAAAC CACTATCATT GAAAATGAGA AACAGACTGT GCAATCAAAG 
    CGGCCAGTCG ATCGACCAAT TCGATGTCCA TTCGCTGTTC AGTTACGCAA CTACAAGGAT
    GGCTCGACCC ACCAGGACTC ACTTCATCAC AAAGCGGCTA AGAATACGCC CTGCAATTCA
    AAAACTTGTG AGGGCTCCAT TATGAATCCC AAACCTCTGA TCCGCGGCCC ATCCAAAATC
    ACACCTTCCC CAGATGACAT GCTCAGCCAG GCAGAGGATT TCATCGACCA GTACTACAAG
    TCGCTCAAAA TCCCGGGCTC GACGGAGCAG CAAGCGCGGA TTGACAGGAT CGCGCTGGAG
    ATCACTCTGA CCGGGACCTA CTCTCTCACC ACCGAGGAAC TCGCTTTTGG GGCCAAACAG
    GCGTGGAGAA ATGCGCCAAG ATGCATTGGC AGGATCCAGT GGTCCAATTT ACAGCTTTTC
    GATGCCCGTG ATTGTAAAAC CGCTGAGGAC ATGTTTCATG CCTTGTGCAC TCATCTTAAG
    TATGCCACCA ACGGAGGAAA CTTGAGGTCT GCCATCACCG TGTTCCCTCA GAGAACAGAT
    GAAGGGCATG ATTTCCGTGT TTGGAACGGT CAGCTGATAA AGTATGCTGG CTATCAGATG
    GATGATGGTA GTGTGATCGG CGACCCAGCG GGTGTAGACT TTACACAGGT GTGTATTCAG
    CTTGGGTGGA AACCAAAGTA CGGTCGCTTT GATGTGCTTC CACTAGTCCT GCAGGCGAAT
    GGAGAAGACC CTGAAGTTTT TGAAATTCCT CCTGAACTGA TCTTGGAGGT CCCCATTGAA
    CATCCAGAGT ATGAATGGTT CAAAGATCTA GACCTGAAGT GGTACGCGAT TCCTGCAGTG
    GCCAACATGC TCATGGAAGT TGGTGGTCTG GAATTCACAG CATGTCCTTT CAACGGCTGG
    TACATGGGCA CAGAAATTGG AGTTCGGGAC TTCTGTGACA GTCAACGCTA CAACATTTTG
    GAGCGCGTTG GCCAGTTAAT GGGCTTAGAA ACACGCAAGC TCTCATCACT GTGGAAAGAC
    CAGGCACTCG TGGCTATCAA TGTTGCCGTC ATTCACAGCT ATCAGAAAAA TAAGGTCACC
    ATCACAGATC ACCATTCCGC AAGCGAGTCC TTCATGCAGC ATTTGGAGAC GGAGGTTCGT
    TTGCGTGGAG GCTGCCCAGC GGATTGGGTC TGGCTGGTGC CACCGATGTC TGGTTCCCTG
    ACCCCCGTCT ATCATCAGGA GTTGCTCAAT TACATCCTGT CCCCATTCTT CTATTACCAG
    ACTGATCCAT GGCTAACACA CAAGTGGAAA GATGAGAAGA AAGGACTTCG GAGATGCAAG
    GTCAGCTTCA AAGGACTGAT CAGAGCTGTA TTTTTCTCTC AAACACTCAT CAAAGCTGTC
    CTGGCCAAGA GAGTGCGCTG TACTGTTCTC TATGCCACAG AAACAGGGAA GTCACTGAGC
    TTTGCCAAAA AACTGAACAC CATGCTCAAC CGGGCCTTTG ACTCCAGGCT GGTATGCATG
    GAGGAATATG ACTTCAGTGA ACTTGAGACA GAGACCCTTC TGATGGTGGT CACCAGTACC
    TTTGGCAATG GAGAAAGTCC AGGAAATGGA GTGACCTTCA GGAAGCAGCT TTTTAGCTTG
    CAAAAAGTGA AAAACACCAT CAGGTACGGT GTCTTTGGCC TGGGTTCACG TGTATACCCA
    CAGTTCTGTG CCTTTGCCCA TGCTGTGGAT GACAAGCTTG CGGAGCTGGG GGCCAAACGT
    ATGACAGCCA CAGGCGAGGG TGATGAACTA AACGGGCAGG ACGAGGCTTT CTCTGCCTGG
    GCTTGCACTG CTTTTAAGGA TGCTTGTGAG GAGTTTAACA TTAAAAAGCA GCTGAAAGCT
    GTGGAGGAGC AGACGGAAGG CTGGGACCCT CAGAGATACA GAGTCCAGCA GGACAGTTGC
    ACTCTGGATC CCATTACAGC ACTGTCTGCT CTTCACTCTA AAACCGTGTT TCCTATGACT
    CTGAAGAGAA GACAGAAACT GCAAAGTGCT CAGTCAAGTC GTTCTACTAT CCTGGTGGAG
    CTGAAGACCG ATGGAAACAC AGATGCCTTG AAGTTTGCCC CAGGAGATCA TGTGGGGATT
    TTTCCAGAGA ATTCTTCAGA ATTGGTGCAT GGTATCGTGA AGCATCTCCC AGATGCCACT
    CCTGATAACC AGAGCCTTCG TCTGGAGACC CTCTCTGAGT CCAGCCATGA GGGAAAGAGA
    TGGCAGACAG ACGAACGTCT CCCTGCTTGC ACGCTCGTCC AGGCTCTAAC ATACTTCCTG
    GATGTCACGA CTCCACCTTC ACAAAGCCTG CTGCGCAAAC TCTCTACAGT GGCGGATCAG
    GAAGAGGATC GCAAACGACT TGAAGCGCTC GCATCGGACT TCCAGGAATA TTCCACATGG
    AAGGATTTCT ACAGGCCCAC CTTCTTGGAG GTTCTTGAAG AATTTCCCTC TCTGAAAGTC
    CCTTCCGCCT TCATCCTGAG TCAGATTCCT CTGCTGAAGC CCCGTCTGTA CTCCGTCAGC
    TCCTCGCTGG ATCATCATCC CAATGAACTA CACCTAACAA TCTCTGTGGT GGAGTACCAG
    ACCCAGGGTG GAGCAGGCCC AATGCATTTC GGCACTTGCA GCACGTGGTT CAACACCATC
    AAAAGAGGAC ACACAGTTCC CTGCTTTGTT CACCGCTCGG GGGGTTTCCA TCTCCCAGAA
    GACCCCAGCA CTCCTGTTCT CCTGATCGGG GCTGGCAGTG GAATTGCTCC TTTCCGCAGT
    TTCTGGCAGC AGCGCACTCA GGATGTGAAA AAGACAGGTC AGCAAATTAG CCCCATGACC
    CTACTGTTTG GCTGTCGCGA TTCAGAAACA GACCACCTGT ACAAAGTAGA AACCCTGAAG
    TTGAGACTTA ATGGTATTCT GGGAAACGTC AATACAGCTT ATTCACGCCA GCCTGGGAAA
    CCCAAGATGT ACGTCCAGGA TGTTTTGAAG GAGCAGCTTG TTGAAAAGGC TTTGGAGGTT
    CTTCACAAGA GCGCAGGTCA TCTTTATGTC TGCGGTGGAA TGAACATGGC CAAAGATGTG
    GCTCAAACTT TGAAAGATAT CCTGGTCAAC AACACAATCA TGAGCGAGGT AGAAGCTGGA
    GAATATATGG ATATGTTGAA GGTTGAGAAG AGATACCATG AAGACATTTT TGGAGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005165353.1
    CDS151..3390
    Translation

    Target ORF information:

    RefSeq Version XM_005165296.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio nitric oxide synthase 2a, inducible (nos2a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005165296.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGAAGAC AAGCACAAAC CACTATCATT GAAAATGAGA AACAGACTGT GCAATCAAAG 
    CGGCCAGTCG ATCGACCAAT TCGATGTCCA TTCGCTGTTC AGTTACGCAA CTACAAGGAT
    GGCTCGACCC ACCAGGACTC ACTTCATCAC AAAGCGGCTA AGAATACGCC CTGCAATTCA
    AAAACTTGTG AGGGCTCCAT TATGAATCCC AAACCTCTGA TCCGCGGCCC ATCCAAAATC
    ACACCTTCCC CAGATGACAT GCTCAGCCAG GCAGAGGATT TCATCGACCA GTACTACAAG
    TCGCTCAAAA TCCCGGGCTC GACGGAGCAG CAAGCGCGGA TTGACAGGAT CGCGCTGGAG
    ATCACTCTGA CCGGGACCTA CTCTCTCACC ACCGAGGAAC TCGCTTTTGG GGCCAAACAG
    GCGTGGAGAA ATGCGCCAAG ATGCATTGGC AGGATCCAGT GGTCCAATTT ACAGCTTTTC
    GATGCCCGTG ATTGTAAAAC CGCTGAGGAC ATGTTTCATG CCTTGTGCAC TCATCTTAAG
    TATGCCACCA ACGGAGGAAA CTTGAGGTCT GCCATCACCG TGTTCCCTCA GAGAACAGAT
    GAAGGGCATG ATTTCCGTGT TTGGAACGGT CAGCTGATAA AGTATGCTGG CTATCAGATG
    GATGATGGTA GTGTGATCGG CGACCCAGCG GGTGTAGACT TTACACAGGT GTGTATTCAG
    CTTGGGTGGA AACCAAAGTA CGGTCGCTTT GATGTGCTTC CACTAGTCCT GCAGGCGAAT
    GGAGAAGACC CTGAAGTTTT TGAAATTCCT CCTGAACTGA TCTTGGAGGT CCCCATTGAA
    CATCCAGAGT ATGAATGGTT CAAAGATCTA GACCTGAAGT GGTACGCGAT TCCTGCAGTG
    GCCAACATGC TCATGGAAGT TGGTGGTCTG GAATTCACAG CATGTCCTTT CAACGGCTGG
    TACATGGGCA CAGAAATTGG AGTTCGGGAC TTCTGTGACA GTCAACGCTA CAACATTTTG
    GAGCGCGTTG GCCAGTTAAT GGGCTTAGAA ACACGCAAGC TCTCATCACT GTGGAAAGAC
    CAGGCACTCG TGGCTATCAA TGTTGCCGTC ATTCACAGCT ATCAGAAAAA TAAGGTCACC
    ATCACAGATC ACCATTCCGC AAGCGAGTCC TTCATGCAGC ATTTGGAGAC GGAGGTTCGT
    TTGCGTGGAG GCTGCCCAGC GGATTGGGTC TGGCTGGTGC CACCGATGTC TGGTTCCCTG
    ACCCCCGTCT ATCATCAGGA GTTGCTCAAT TACATCCTGT CCCCATTCTT CTATTACCAG
    ACTGATCCAT GGCTAACACA CAAGTGGAAA GATGAGAAGA AAGGACTTCG GAGATGCAAG
    GTCAGCTTCA AAGGACTGAT CAGAGCTGTA TTTTTCTCTC AAACACTCAT CAAAGCTGTC
    CTGGCCAAGA GAGTGCGCTG TACTGTTCTC TATGCCACAG AAACAGGGAA GTCACTGAGC
    TTTGCCAAAA AACTGAACAC CATGCTCAAC CGGGCCTTTG ACTCCAGGCT GGTATGCATG
    GAGGAATATG ACTTCAGTGA ACTTGAGACA GAGACCCTTC TGATGGTGGT CACCAGTACC
    TTTGGCAATG GAGAAAGTCC AGGAAATGGA GTGACCTTCA GGAAGCAGCT TTTTAGCTTG
    CAAAAAGTGA AAAACACCAT CAGGTACGGT GTCTTTGGCC TGGGTTCACG TGTATACCCA
    CAGTTCTGTG CCTTTGCCCA TGCTGTGGAT GACAAGCTTG CGGAGCTGGG GGCCAAACGT
    ATGACAGCCA CAGGCGAGGG TGATGAACTA AACGGGCAGG ACGAGGCTTT CTCTGCCTGG
    GCTTGCACTG CTTTTAAGGA TGCTTGTGAG GAGTTTAACA TTAAAAAGCA GCTGAAAGCT
    GTGGAGGAGC AGACGGAAGG CTGGGACCCT CAGAGATACA GAGTCCAGCA GGACAGTTGC
    ACTCTGGATC CCATTACAGC ACTGTCTGCT CTTCACTCTA AAACCGTGTT TCCTATGACT
    CTGAAGAGAA GACAGAAACT GCAAAGTGCT CAGTCAAGTC GTTCTACTAT CCTGGTGGAG
    CTGAAGACCG ATGGAAACAC AGATGCCTTG AAGTTTGCCC CAGGAGATCA TGTGGGGATT
    TTTCCAGAGA ATTCTTCAGA ATTGGTGCAT GGTATCGTGA AGCATCTCCC AGATGCCACT
    CCTGATAACC AGAGCCTTCG TCTGGAGACC CTCTCTGAGT CCAGCCATGA GGGAAAGAGA
    TGGCAGACAG ACGAACGTCT CCCTGCTTGC ACGCTCGTCC AGGCTCTAAC ATACTTCCTG
    GATGTCACGA CTCCACCTTC ACAAAGCCTG CTGCGCAAAC TCTCTACAGT GGCGGATCAG
    GAAGAGGATC GCAAACGACT TGAAGCGCTC GCATCGGACT TCCAGGAATA TTCCACATGG
    AAGGATTTCT ACAGGCCCAC CTTCTTGGAG GTTCTTGAAG AATTTCCCTC TCTGAAAGTC
    CCTTCCGCCT TCATCCTGAG TCAGATTCCT CTGCTGAAGC CCCGTCTGTA CTCCGTCAGC
    TCCTCGCTGG ATCATCATCC CAATGAACTA CACCTAACAA TCTCTGTGGT GGAGTACCAG
    ACCCAGGGTG GAGCAGGCCC AATGCATTTC GGCACTTGCA GCACGTGGTT CAACACCATC
    AAAAGAGGAC ACACAGTTCC CTGCTTTGTT CACCGCTCGG GGGGTTTCCA TCTCCCAGAA
    GACCCCAGCA CTCCTGTTCT CCTGATCGGG GCTGGCAGTG GAATTGCTCC TTTCCGCAGT
    TTCTGGCAGC AGCGCACTCA GGATGTGAAA AAGACAGGTC AGCAAATTAG CCCCATGACC
    CTACTGTTTG GCTGTCGCGA TTCAGAAACA GACCACCTGT ACAAAGTAGA AACCCTGAAG
    TTGAGACTTA ATGGTATTCT GGGAAACGTC AATACAGCTT ATTCACGCCA GCCTGGGAAA
    CCCAAGATGT ACGTCCAGGA TGTTTTGAAG GAGCAGCTTG TTGAAAAGGC TTTGGAGGTT
    CTTCACAAGA GCGCAGGTCA TCTTTATGTC TGCGGTGGAA TGAACATGGC CAAAGATGTG
    GCTCAAACTT TGAAAGATAT CCTGGTCAAC AACACAATCA TGAGCGAGGT AGAAGCTGGA
    GAATATATGG ATATGTTGAA GGTTGAGAAG AGATACCATG AAGACATTTT TGGAGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.