NOS2 cDNA ORF clone, Bos taurus(cattle)

The following NOS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa01375 NM_001076799.1
Latest version!
Bos taurus nitric oxide synthase 2 (NOS2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa226012 XM_024979645.1
Latest version!
Bos taurus nitric oxide synthase 2 (NOS2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa226013 XM_024979646.1
Latest version!
Bos taurus nitric oxide synthase 2 (NOS2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa01375
    Clone ID Related Accession (Same CDS sequence) NM_001076799.1
    Accession Version NM_001076799.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Bos taurus(cattle)
    Product nitric oxide synthase, inducible
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ676956.1. On or before Oct 11, 2006 this sequence version replaced XM_588018.2, XM_880927.1, XM_880948.1, XM_880967.1, XM_880990.1, XM_881006.1, XM_881023.1, XM_881043.1, XM_881060.1, XM_867963.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ676956.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCTGTC CCTGGCAGTT TCTGTTCAAA ATCAAATCCC AAAAGGTGGA CTTGGCTACG 
    GAACTGGACA TCAACAACAA TGTGGGAAAA TTCTACCAGC CCCCGTCCAG TCCAGTGACA
    CAGGATGACC CCAAACGTCA CAGCCCGGGC AAGCACGGGA ATGAGTCTCC CCAGCCCCTC
    ACGGGGACAG TAAAGACGTC TCCAGAATCC CTGAGCAAGC TGGACGCGCC CCCGTCAGCC
    TGTCCACGGC ATGTGAGGAT CAAAAACTGG GGCAGCGGAG TGACTTTCCA AGACACGCTT
    CACCAGAAGG CCAAAGGGGA TCTCTCTTGC AAGTCCAAGT CTTGCCTGGC ATCCATCATG
    AACCCCAAAA GTTTGACCAT AGGACCCCGG GACAAGCCAA CCCCCCCAGA CGAGCTTCTA
    CCTCAAGCTA TCGAATTTGT CAACCAGTAT TACGGCTCCT TCAAAGAGGC AAAAATAGAG
    GAACATCTGG CCAGGGTGGA AGCAGTAACA AAGGAGATAG AAACAACAGG AACCTACCAG
    CTGACGGGAG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAACGC CCCCCGCTGC
    ATCGGAAGGA TCCAGTGGTC GAACCTGCAG GTCTTTGACG CCCGGAGCTG TTCCACGGCC
    CAGGAAATGT TCGAACACAT CTGCAGACAC GTGCGTTATG CCACCAACAA CGGCAACATC
    AGGTCGGCCA TCACTGTGTT CCCCCAGCGG AGCGATGGGA AGCATGACTT CCGGGTCTGG
    AACGCCCAGC TCATCCGCTA TGCCGGCTAC CAGATGCCAG ATGGCAGCAT CAGAGGGGAC
    CCCGCCAACG TGGAGTTCAC ACAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC
    CGCTTCGACG TGTTGCCTCT AGTCCTGCAG GCTGACGGCC GTGACCCAGA GCTCTTCGAA
    ATCCCCCCTG ACCTTGTTCT CGAGGTGCCC ATGGAACATC CCAGGTACGA ATGGTTCCGG
    GAGCTGGAGC TCAAGTGGTA CGCCCTGCCA GCCGTGGCCA ACATGCTGCT TGAGGTGGGC
    GGCCTCGAGT TCCCAGGGTG CCCCTTCAAC GGGTGGTACA TGGGCACAGA AGTTGGAGTC
    CGGGACTTCT GCGACGCCCA GCGATACAAC ATCCTGGAGG AAGTGGGCAG AAGGATGGGC
    CTGGAAACGC ACAAGGTGGC CTCACTCTGG AAGGACCGGG CTGTCGTTGA GATCAACGTC
    GCTGTGCTCC ACAGTTTCCA GAAGCAGAAC GTGACCATCA TGGACCACCA CTCCGCTGCA
    GAGTCCTTCA TGAAGTACAT GCAGAATGAG TACCGCTCCC GAGGGGGCTG CCCAGCTGAC
    TGGATTTGGC TGGTCCCCCC GATCTCTGGA AGCATCACCC CCGTGTTCCA CCAGGAGATG
    TTAAATTACG TCCTGTCCCC TTTCTACTAC TACCAGGTGG AGCCCTGGAA AACCCACGTC
    TGGCAGGACG AGAGGCGGAG ACCCCAGAGA AGAGAAATTC GATTCAAAGT CTTGGTCAAA
    GCCGTGTTCT TCGCCTCGGT GCTGATGCAT AAGGCCATGG CATCCCGGGT CAGAGCCACG
    ATCCTCTTTG CAACAGAGAC GGGGAGATCG GAAACGCTGG CCCAGGACCT GGGGGCTTTG
    TTCAGCTGTG CCTTCAACCC CAAGGTTCTC TGCATGGATC AGTACCAGCT GAGCCATCTG
    GAGGAGGARC AGCTGCTGCT GGTGGTGACC AGCACTTTTG GCAACGGAGA CTCCCCTGGC
    AACGGAGAGA AACTGAAGAA GTCCCTCTTG ATGCTGAAAG AACTCACCAA CACGTTCCGG
    TACGCCGTGT TTGGCCTTGG CTCCAGCATG TACCCTCAGT TCTGCGCTTT TGCTCATGAC
    ATTGACCAGA AGTTGTCCCA GCTGGGGGCT TCTCAGCTTG CCCCAACCGG GGAAGGGGAC
    GAACTCAGCG GGCAGGAGGA GGCCTTTCGC AGCTGGGCCG TGCAAACCTT CAAGGCAGCC
    TGTGAGACAT TCGATGTCAG CGGCAAGCAC CACATTGAGA TCCCCAAGCT CTACACTTCC
    AATGTGACCT GGGACCCGCA GCACTACAGG CTCGTGCAGG ACTCAGAGCC TCTGGACCTC
    AACAAAGCCC TGAGCAGTAT GCACGCCAAG CATGTGTTCA CCATGAGGCT CAAATCGCAG
    CAGAATCTGC AGAGTCCGAA ATCCAGCCGC ACCACCCTCC TGGTGGAACT CTCCTGTGAG
    GGCAGCCAAG CTCCAAGCTA CCTGCCGGGA GAGCACCTCG GAGTTTTCCC ATGCAACCAG
    CCGGCCCTGG TTCAAGGCAT CCTTGAGCGA GTGGTGGATG GCCCTGCCCC GCACCAGCCT
    GTGCGCCTGG AGACCCTCTG TGAGAATGGC AGCTATTGGG TCAAGGATAA GCGGCTGCCA
    CCTTGCTCAC TCAGCCAGGC TCTCACCTAT TTCCTGGACA TCACCACCCC GCCAACCCAG
    CTGCTGCTCC GAAAGCTGGC CCAGCTGGCC ACAGAAGAGG CTGAGAAGCA GAGGCTGGAG
    ACCCTATGCC AGCCCTCAGA TTACAACAAG TGGAAGTTCA CCAACAGCCC CACATTTCTG
    GAGGTGCTGG AGGAGTTCCC GTCCCTGCGG GTGTCTGCCA GCTTCCTGCT CTCCCAGCTC
    CCTATTCTGA AGCCCCGGTA CTACTCCATC AGCTCCTCCA GGGACCTCAC ACCCACGGAG
    ATCCACCTCA CTGTGGCCGT GCTCACGTAC CGCACCCGAG ATGGCCAAGG TCCCCTGCAC
    CATGGTGTCT GCAGCACGTG GCTCAGCAGC TTGAAGCCCC AAGACCCGGT GCCCTGCTTT
    GTGCGAAGTG CCAGYGGCTT CCAGCTTCCC GAGGACCGCT CCCGTCCTTG CATCCTCATT
    GGGCCCGGCA CGGGCATCGC CCCCTTCCGC AGTTTCTGGC AGCAGCGGCT CCATGAGGCT
    GAGCACAAAG GGCTCCAGGG CGGCCGCATG ACCCTGGTTT TCGGGTGCCG CCGCCCAGAA
    GAGGACCACC TCTACTGGGA GGAGATGCTG GAGATGGCCC GCAAGGGGGT GCTGCACGAG
    GTGCACACCG CCTATTCCCG CCTGCCTGAC CAGCCCAAGG TCTATGTTCA AGACATCCTG
    CGGCAGCGGC TGGCGGGCGA GGTGCTTCGC GTGCTCCATG AGGAGCAAGG TCACCTCTAT
    GTCTGTGGGG ATGTGCGCAT GGCCCGGGAC GTGGCCCGCA CCCTGAAGCA GCTGATGGCT
    ACTGCGCTGA GCCTGAACGA GGAACAGGTG GAGGACTATT TCTTCCAGCT CAAGAACCAG
    AAGCGCTATC ATGAAGACAT CTTTGGTGCT GTATTTCCCT ATGAGGTGAA AAAGGATGGG
    GCAGCAGGGC TGCCCAGCAA TCCCAGAGCT CCAGGCGCCC ACAGAAGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001070267.1
    CDS1..3471
    Translation

    Target ORF information:

    RefSeq Version NM_001076799.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nitric oxide synthase 2 (NOS2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001076799.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCTGTC CCTGGCAGTT TCTGTTCAAA ATCAAATCCC AAAAGGTGGA CTTGGCTACG 
    GAACTGGACA TCAACAACAA TGTGGGAAAA TTCTACCAGC CCCCGTCCAG TCCAGTGACA
    CAGGATGACC CCAAACGTCA CAGCCCGGGC AAGCACGGGA ATGAGTCTCC CCAGCCCCTC
    ACGGGGACAG TAAAGACGTC TCCAGAATCC CTGAGCAAGC TGGACGCGCC CCCGTCAGCC
    TGTCCACGGC ATGTGAGGAT CAAAAACTGG GGCAGCGGAG TGACTTTCCA AGACACGCTT
    CACCAGAAGG CCAAAGGGGA TCTCTCTTGC AAGTCCAAGT CTTGCCTGGC ATCCATCATG
    AACCCCAAAA GTTTGACCAT AGGACCCCGG GACAAGCCAA CCCCCCCAGA CGAGCTTCTA
    CCTCAAGCTA TCGAATTTGT CAACCAGTAT TACGGCTCCT TCAAAGAGGC AAAAATAGAG
    GAACATCTGG CCAGGGTGGA AGCAGTAACA AAGGAGATAG AAACAACAGG AACCTACCAG
    CTGACGGGAG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAACGC CCCCCGCTGC
    ATCGGAAGGA TCCAGTGGTC GAACCTGCAG GTCTTTGACG CCCGGAGCTG TTCCACGGCC
    CAGGAAATGT TCGAACACAT CTGCAGACAC GTGCGTTATG CCACCAACAA CGGCAACATC
    AGGTCGGCCA TCACTGTGTT CCCCCAGCGG AGCGATGGGA AGCATGACTT CCGGGTCTGG
    AACGCCCAGC TCATCCGCTA TGCCGGCTAC CAGATGCCAG ATGGCAGCAT CAGAGGGGAC
    CCCGCCAACG TGGAGTTCAC ACAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC
    CGCTTCGACG TGTTGCCTCT AGTCCTGCAG GCTGACGGCC GTGACCCAGA GCTCTTCGAA
    ATCCCCCCTG ACCTTGTTCT CGAGGTGCCC ATGGAACATC CCAGGTACGA ATGGTTCCGG
    GAGCTGGAGC TCAAGTGGTA CGCCCTGCCA GCCGTGGCCA ACATGCTGCT TGAGGTGGGC
    GGCCTCGAGT TCCCAGGGTG CCCCTTCAAC GGGTGGTACA TGGGCACAGA AGTTGGAGTC
    CGGGACTTCT GCGACGCCCA GCGATACAAC ATCCTGGAGG AAGTGGGCAG AAGGATGGGC
    CTGGAAACGC ACAAGGTGGC CTCACTCTGG AAGGACCGGG CTGTCGTTGA GATCAACGTC
    GCTGTGCTCC ACAGTTTCCA GAAGCAGAAC GTGACCATCA TGGACCACCA CTCCGCTGCA
    GAGTCCTTCA TGAAGTACAT GCAGAATGAG TACCGCTCCC GAGGGGGCTG CCCAGCTGAC
    TGGATTTGGC TGGTCCCCCC GATCTCTGGA AGCATCACCC CCGTGTTCCA CCAGGAGATG
    TTAAATTACG TCCTGTCCCC TTTCTACTAC TACCAGGTGG AGCCCTGGAA AACCCACGTC
    TGGCAGGACG AGAGGCGGAG ACCCCAGAGA AGAGAAATTC GATTCAAAGT CTTGGTCAAA
    GCCGTGTTCT TCGCCTCGGT GCTGATGCAT AAGGCCATGG CATCCCGGGT CAGAGCCACG
    ATCCTCTTTG CAACAGAGAC GGGGAGATCG GAAACGCTGG CCCAGGACCT GGGGGCTTTG
    TTCAGCTGTG CCTTCAACCC CAAGGTTCTC TGCATGGATC AGTACCAGCT GAGCCATCTG
    GAGGAGGARC AGCTGCTGCT GGTGGTGACC AGCACTTTTG GCAACGGAGA CTCCCCTGGC
    AACGGAGAGA AACTGAAGAA GTCCCTCTTG ATGCTGAAAG AACTCACCAA CACGTTCCGG
    TACGCCGTGT TTGGCCTTGG CTCCAGCATG TACCCTCAGT TCTGCGCTTT TGCTCATGAC
    ATTGACCAGA AGTTGTCCCA GCTGGGGGCT TCTCAGCTTG CCCCAACCGG GGAAGGGGAC
    GAACTCAGCG GGCAGGAGGA GGCCTTTCGC AGCTGGGCCG TGCAAACCTT CAAGGCAGCC
    TGTGAGACAT TCGATGTCAG CGGCAAGCAC CACATTGAGA TCCCCAAGCT CTACACTTCC
    AATGTGACCT GGGACCCGCA GCACTACAGG CTCGTGCAGG ACTCAGAGCC TCTGGACCTC
    AACAAAGCCC TGAGCAGTAT GCACGCCAAG CATGTGTTCA CCATGAGGCT CAAATCGCAG
    CAGAATCTGC AGAGTCCGAA ATCCAGCCGC ACCACCCTCC TGGTGGAACT CTCCTGTGAG
    GGCAGCCAAG CTCCAAGCTA CCTGCCGGGA GAGCACCTCG GAGTTTTCCC ATGCAACCAG
    CCGGCCCTGG TTCAAGGCAT CCTTGAGCGA GTGGTGGATG GCCCTGCCCC GCACCAGCCT
    GTGCGCCTGG AGACCCTCTG TGAGAATGGC AGCTATTGGG TCAAGGATAA GCGGCTGCCA
    CCTTGCTCAC TCAGCCAGGC TCTCACCTAT TTCCTGGACA TCACCACCCC GCCAACCCAG
    CTGCTGCTCC GAAAGCTGGC CCAGCTGGCC ACAGAAGAGG CTGAGAAGCA GAGGCTGGAG
    ACCCTATGCC AGCCCTCAGA TTACAACAAG TGGAAGTTCA CCAACAGCCC CACATTTCTG
    GAGGTGCTGG AGGAGTTCCC GTCCCTGCGG GTGTCTGCCA GCTTCCTGCT CTCCCAGCTC
    CCTATTCTGA AGCCCCGGTA CTACTCCATC AGCTCCTCCA GGGACCTCAC ACCCACGGAG
    ATCCACCTCA CTGTGGCCGT GCTCACGTAC CGCACCCGAG ATGGCCAAGG TCCCCTGCAC
    CATGGTGTCT GCAGCACGTG GCTCAGCAGC TTGAAGCCCC AAGACCCGGT GCCCTGCTTT
    GTGCGAAGTG CCAGYGGCTT CCAGCTTCCC GAGGACCGCT CCCGTCCTTG CATCCTCATT
    GGGCCCGGCA CGGGCATCGC CCCCTTCCGC AGTTTCTGGC AGCAGCGGCT CCATGAGGCT
    GAGCACAAAG GGCTCCAGGG CGGCCGCATG ACCCTGGTTT TCGGGTGCCG CCGCCCAGAA
    GAGGACCACC TCTACTGGGA GGAGATGCTG GAGATGGCCC GCAAGGGGGT GCTGCACGAG
    GTGCACACCG CCTATTCCCG CCTGCCTGAC CAGCCCAAGG TCTATGTTCA AGACATCCTG
    CGGCAGCGGC TGGCGGGCGA GGTGCTTCGC GTGCTCCATG AGGAGCAAGG TCACCTCTAT
    GTCTGTGGGG ATGTGCGCAT GGCCCGGGAC GTGGCCCGCA CCCTGAAGCA GCTGATGGCT
    ACTGCGCTGA GCCTGAACGA GGAACAGGTG GAGGACTATT TCTTCCAGCT CAAGAACCAG
    AAGCGCTATC ATGAAGACAT CTTTGGTGCT GTATTTCCCT ATGAGGTGAA AAAGGATGGG
    GCAGCAGGGC TGCCCAGCAA TCCCAGAGCT CCAGGCGCCC ACAGAAGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226012
    Clone ID Related Accession (Same CDS sequence) XM_024979645.1
    Accession Version XM_024979645.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nitric oxide synthase, inducible isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCTGTC CCTGGCAGTT TCTGTTCAAA ATCAAATCCC AAAAGGTGGA CTTGGCTACG 
    GAACTGGACA TCAACAACAA TGTGGGAAAA TTCTACCAGC CCCCGTCCAG TCCAGTGACA
    CAGGATGACC CCAAACGTCA CAGCCCGGGC AAGCACGGGA ATGAGTCTCC CCAGCCCCTC
    ACGGGGACAG TAAAGACGTC TCCAGAATCC CTGAGCAAGC TGGACGCGCC CCCGTCAGCC
    TGTCCACGGC ATGTGAGGAT CAAAAACTGG GGCAGCGGAG TGACTTTCCA AGACACGCTT
    CACCAGAAGG CCAAAGGGGA TCTCTCTTGC AAGTCCAAGT CTTGCCTGGC ATCCATCATG
    AACCCCAAAA GTTTGACCAT AGGACCCCGG GACAAGCCAA CCCCCCCAGA CGAGCTTCTA
    CCTCAAGCTA TCGAATTTGT CAACCAGTAT TACGGCTCCT TCAAAGAGGC AAAAATAGAG
    GAACATCTGG CCAGGGTGGA AGCAGTAACA AAGGAGATAG AAACAACAGG AACCTACCAG
    CTGACGGGAG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAACGC CCCCCGCTGC
    ATCGGAAGGA TCCAGTGGTC GAACCTGCAG GTCTTTGACG CCCGGAGCTG TTCCACGGCC
    CAGGAAATGT TCGAACACAT CTGCAGACAC GTGCGTTATG CCACCAACAA CGGCAACATC
    AGGTCGGCCA TCACTGTGTT CCCCCAGCGG AGCGATGGGA AGCATGACTT CCGGGTCTGG
    AACGCCCAGC TCATCCGCTA TGCCGGCTAC CAGATGCCAG ATGGCAGCAT CAGAGGGGAC
    CCCGCCAACG TGGAGTTCAC ACAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC
    CGCTTCGACG TGTTGCCTCT AGTCCTGCAG GCTGACGGCC GTGACCCAGA GCTCTTCGAA
    ATCCCCCCTG ACCTTGTTCT CGAGGTGCCC ATGGAACATC CCAGGTACGA ATGGTTCCGG
    GAGCTGGAGC TCAAGTGGTA CGCCCTGCCA GCCGTGGCCA ACATGCTGCT TGAGGTGGGC
    GGCCTCGAGT TCCCAGGGTG CCCCTTCAAC GGGTGGTACA TGGGCACAGA AGTTGGAGTC
    CGGGACTTCT GCGACGCCCA GCGATACAAC ATCCTGGAGG AAGTGGGCAG AAGGATGGGC
    CTGGAAACGC ACAAGGTGGC CTCACTCTGG AAGGACCGGG CTGTCGTTGA GATCAACGTC
    GCTGTGCTCC ACAGTTTCCA GAAGCAGAAC GTGACCATCA TGGACCACCA CTCCGCTGCA
    GAGTCCTTCA TGAAGTACAT GCAGAATGAG TACCGCTCCC GAGGGGGCTG CCCAGCTGAC
    TGGATTTGGC TGGTCCCCCC GATCTCTGGA AGCATCACCC CCGTGTTCCA CCAGGAGATG
    TTAAATTACG TCCTGTCCCC TTTCTACTAC TACCAGGTGG AGCCCTGGAA AACCCACGTC
    TGGCAGGACG AGAGGCGGAG ACCCCAGAGA AGAGAGATTC GATTCAAAGT CTTGGTCAAA
    GCCGTGTTCT TCGCCTCGGT GCTGATGCAT AAGACCATGG CATCCCGGGT CAGAGCCACG
    ATCCTCTTTG CAACAGAGAC GGGGAGATCG GAAACGCTGG CCCAGGACCT GGGGGCTTTG
    TTCAGCTGTG CCTTCAACCC CAAGGTTCTC TGCATGGATC AGTACCAGCT GAGCCATCTG
    GAGGAGGAGC AGCTGCTGCT GGTGGTGACC AGCACTTTTG GCAACGGAGA CTCCCCTGGC
    AACGGAGAGA AACTGAAGAA GTCCCTCTTG ATGCTGAAAG AACTCACCAA CACGTTCCGG
    TACGCCGTGT TTGGCCTTGG CTCCAGCATG TACCCTCAGT TCTGCGCTTT TGCTCATGAC
    ATTGACCAGA AGTTGTCCCA GCTGGGGGCT TCTCAGCTTG TCCCAACCGG GGAAGGGGAC
    GAACTCAGCG GGCAGGAGGA GGCCTTTCGC AGCTGGGCCG TGCAAACCTT CAAGGCAGCC
    TGTGAGACAT TCGATGTCAG CGGCAAGCAC CACATTGAGA TCCCCAAGCT CTACACTTCC
    AATGTGACCT GGGACCCGCA GCACTACAGG CTCGTGCAGG ACTCAGAGCC TCTGGACCTC
    AACAAAGCCC TGAGCAGTAT GCACGCCAAG CATGTGTTCA CCATGAGGCT CAAATCGCAG
    CAGAATCTGC AGAGTCCGAA ATCCAGCCGC ACCACCCTCC TGGTGGAACT CTCCTGTGAG
    GGCAGCCAAG CTCCAAGCTA CCTGCCGGGA GAGCACCTCG GAGTTTTCCC ATGCAACCAG
    CCGGCCCTGG TTCAAGGCAT CCTTGAGCGA GTGGTGGATG GCCCTGCCCC GCACCAGCCT
    GTGCGCCTGG AGACCCTCTG TGAGAATGGC AGCTATTGGG TCAAGGATAA GCGGCTGCCG
    CCTTGCTCAC TCAGCCAGGC TCTCACCTAT TTCCTGGACA TCACCACCCC GCCAACCCAG
    CTGCTGCTCC GAAAGCTGGC CCAGCTGGCC ACAGAAGAGG CTGAGAAGCA GAGGCTGGAG
    ACCCTATGCC AGCCCTCAGA TTACAACAAG TGGAAGTTCA CCAACAGCCC CACATTTCTG
    GAGGTGCTGG AGGAGTTCCC GTCCCTGCGG GTGTCTGCCA GCTTCCTGCT CTCCCAGCTC
    CCTATTCTGA AGCCCCGGTA CTACTCCATC AGCTCCTCCA GGGACCTCAC ACCCACGGAG
    ATCCACCTCA CTGTGGCCGT GCTCACGTAC CGCACCCGAG ATGGCCAAGG TCCCCTGCAC
    CATGGTGTCT GCAGCACGTG GCTCAGCAGC TTGAAGCCCC AAGACCCGGT GCCCTGCTTT
    GTGCGAAGTG CCAGCGGCTT CCAGCTTCCC GAGGACCGCT CCCGTCCTTG CATCCTCATT
    GGGCCCGGCA CGGGCATCGC CCCCTTCCGC AGTTTCTGGC AGCAGCGGCT CCATGAGGCT
    GAGCACAAAG GGCTCCAGGG CGGCCGCATG ACCCTGGTTT TCGGGTGCCG CCGCCCAGAA
    GAGGACCACC TCTACTGGGA GGAGATGCTG GAGATGGCCC GCAAGGGGGT GCTGCACGAG
    GTGCACACCG CCTATTCCCG CCTGCCTGAC CAGCCCAAGG TCTATGTTCA AGACATCCTG
    CGGCAGCGGC TGGCGGGCGA GGTGCTTCGC GTGCTCCATG AGGAGCAAGG TCACCTCTAT
    GTCTGTGGGG ATGTGCGCAT GGCCCGGGAC GTGGCCCGCA CCCTGAAGCA GCTGATGGCT
    ACTGCGCTGA GCCTGAACGA GGAACAGGTG GAGGACTATT TCTTCCAGCT CAAGAACCAG
    AAGCGCTATC ATGAAGACAT CTTTGGTGCT GTATTTCCCT ATGAGGTGAA AAAGGATGGG
    GCAGCAGGGC TGCCCAGCAA TCCCAGAGCT CCAGGCGCCC ACAGAAGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835413.1
    CDS326..3796
    Translation

    Target ORF information:

    RefSeq Version XM_024979645.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nitric oxide synthase 2 (NOS2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979645.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCTGTC CCTGGCAGTT TCTGTTCAAA ATCAAATCCC AAAAGGTGGA CTTGGCTACG 
    GAACTGGACA TCAACAACAA TGTGGGAAAA TTCTACCAGC CCCCGTCCAG TCCAGTGACA
    CAGGATGACC CCAAACGTCA CAGCCCGGGC AAGCACGGGA ATGAGTCTCC CCAGCCCCTC
    ACGGGGACAG TAAAGACGTC TCCAGAATCC CTGAGCAAGC TGGACGCGCC CCCGTCAGCC
    TGTCCACGGC ATGTGAGGAT CAAAAACTGG GGCAGCGGAG TGACTTTCCA AGACACGCTT
    CACCAGAAGG CCAAAGGGGA TCTCTCTTGC AAGTCCAAGT CTTGCCTGGC ATCCATCATG
    AACCCCAAAA GTTTGACCAT AGGACCCCGG GACAAGCCAA CCCCCCCAGA CGAGCTTCTA
    CCTCAAGCTA TCGAATTTGT CAACCAGTAT TACGGCTCCT TCAAAGAGGC AAAAATAGAG
    GAACATCTGG CCAGGGTGGA AGCAGTAACA AAGGAGATAG AAACAACAGG AACCTACCAG
    CTGACGGGAG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAACGC CCCCCGCTGC
    ATCGGAAGGA TCCAGTGGTC GAACCTGCAG GTCTTTGACG CCCGGAGCTG TTCCACGGCC
    CAGGAAATGT TCGAACACAT CTGCAGACAC GTGCGTTATG CCACCAACAA CGGCAACATC
    AGGTCGGCCA TCACTGTGTT CCCCCAGCGG AGCGATGGGA AGCATGACTT CCGGGTCTGG
    AACGCCCAGC TCATCCGCTA TGCCGGCTAC CAGATGCCAG ATGGCAGCAT CAGAGGGGAC
    CCCGCCAACG TGGAGTTCAC ACAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC
    CGCTTCGACG TGTTGCCTCT AGTCCTGCAG GCTGACGGCC GTGACCCAGA GCTCTTCGAA
    ATCCCCCCTG ACCTTGTTCT CGAGGTGCCC ATGGAACATC CCAGGTACGA ATGGTTCCGG
    GAGCTGGAGC TCAAGTGGTA CGCCCTGCCA GCCGTGGCCA ACATGCTGCT TGAGGTGGGC
    GGCCTCGAGT TCCCAGGGTG CCCCTTCAAC GGGTGGTACA TGGGCACAGA AGTTGGAGTC
    CGGGACTTCT GCGACGCCCA GCGATACAAC ATCCTGGAGG AAGTGGGCAG AAGGATGGGC
    CTGGAAACGC ACAAGGTGGC CTCACTCTGG AAGGACCGGG CTGTCGTTGA GATCAACGTC
    GCTGTGCTCC ACAGTTTCCA GAAGCAGAAC GTGACCATCA TGGACCACCA CTCCGCTGCA
    GAGTCCTTCA TGAAGTACAT GCAGAATGAG TACCGCTCCC GAGGGGGCTG CCCAGCTGAC
    TGGATTTGGC TGGTCCCCCC GATCTCTGGA AGCATCACCC CCGTGTTCCA CCAGGAGATG
    TTAAATTACG TCCTGTCCCC TTTCTACTAC TACCAGGTGG AGCCCTGGAA AACCCACGTC
    TGGCAGGACG AGAGGCGGAG ACCCCAGAGA AGAGAGATTC GATTCAAAGT CTTGGTCAAA
    GCCGTGTTCT TCGCCTCGGT GCTGATGCAT AAGACCATGG CATCCCGGGT CAGAGCCACG
    ATCCTCTTTG CAACAGAGAC GGGGAGATCG GAAACGCTGG CCCAGGACCT GGGGGCTTTG
    TTCAGCTGTG CCTTCAACCC CAAGGTTCTC TGCATGGATC AGTACCAGCT GAGCCATCTG
    GAGGAGGAGC AGCTGCTGCT GGTGGTGACC AGCACTTTTG GCAACGGAGA CTCCCCTGGC
    AACGGAGAGA AACTGAAGAA GTCCCTCTTG ATGCTGAAAG AACTCACCAA CACGTTCCGG
    TACGCCGTGT TTGGCCTTGG CTCCAGCATG TACCCTCAGT TCTGCGCTTT TGCTCATGAC
    ATTGACCAGA AGTTGTCCCA GCTGGGGGCT TCTCAGCTTG TCCCAACCGG GGAAGGGGAC
    GAACTCAGCG GGCAGGAGGA GGCCTTTCGC AGCTGGGCCG TGCAAACCTT CAAGGCAGCC
    TGTGAGACAT TCGATGTCAG CGGCAAGCAC CACATTGAGA TCCCCAAGCT CTACACTTCC
    AATGTGACCT GGGACCCGCA GCACTACAGG CTCGTGCAGG ACTCAGAGCC TCTGGACCTC
    AACAAAGCCC TGAGCAGTAT GCACGCCAAG CATGTGTTCA CCATGAGGCT CAAATCGCAG
    CAGAATCTGC AGAGTCCGAA ATCCAGCCGC ACCACCCTCC TGGTGGAACT CTCCTGTGAG
    GGCAGCCAAG CTCCAAGCTA CCTGCCGGGA GAGCACCTCG GAGTTTTCCC ATGCAACCAG
    CCGGCCCTGG TTCAAGGCAT CCTTGAGCGA GTGGTGGATG GCCCTGCCCC GCACCAGCCT
    GTGCGCCTGG AGACCCTCTG TGAGAATGGC AGCTATTGGG TCAAGGATAA GCGGCTGCCG
    CCTTGCTCAC TCAGCCAGGC TCTCACCTAT TTCCTGGACA TCACCACCCC GCCAACCCAG
    CTGCTGCTCC GAAAGCTGGC CCAGCTGGCC ACAGAAGAGG CTGAGAAGCA GAGGCTGGAG
    ACCCTATGCC AGCCCTCAGA TTACAACAAG TGGAAGTTCA CCAACAGCCC CACATTTCTG
    GAGGTGCTGG AGGAGTTCCC GTCCCTGCGG GTGTCTGCCA GCTTCCTGCT CTCCCAGCTC
    CCTATTCTGA AGCCCCGGTA CTACTCCATC AGCTCCTCCA GGGACCTCAC ACCCACGGAG
    ATCCACCTCA CTGTGGCCGT GCTCACGTAC CGCACCCGAG ATGGCCAAGG TCCCCTGCAC
    CATGGTGTCT GCAGCACGTG GCTCAGCAGC TTGAAGCCCC AAGACCCGGT GCCCTGCTTT
    GTGCGAAGTG CCAGCGGCTT CCAGCTTCCC GAGGACCGCT CCCGTCCTTG CATCCTCATT
    GGGCCCGGCA CGGGCATCGC CCCCTTCCGC AGTTTCTGGC AGCAGCGGCT CCATGAGGCT
    GAGCACAAAG GGCTCCAGGG CGGCCGCATG ACCCTGGTTT TCGGGTGCCG CCGCCCAGAA
    GAGGACCACC TCTACTGGGA GGAGATGCTG GAGATGGCCC GCAAGGGGGT GCTGCACGAG
    GTGCACACCG CCTATTCCCG CCTGCCTGAC CAGCCCAAGG TCTATGTTCA AGACATCCTG
    CGGCAGCGGC TGGCGGGCGA GGTGCTTCGC GTGCTCCATG AGGAGCAAGG TCACCTCTAT
    GTCTGTGGGG ATGTGCGCAT GGCCCGGGAC GTGGCCCGCA CCCTGAAGCA GCTGATGGCT
    ACTGCGCTGA GCCTGAACGA GGAACAGGTG GAGGACTATT TCTTCCAGCT CAAGAACCAG
    AAGCGCTATC ATGAAGACAT CTTTGGTGCT GTATTTCCCT ATGAGGTGAA AAAGGATGGG
    GCAGCAGGGC TGCCCAGCAA TCCCAGAGCT CCAGGCGCCC ACAGAAGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226013
    Clone ID Related Accession (Same CDS sequence) XM_024979646.1
    Accession Version XM_024979646.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3468bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nitric oxide synthase, inducible isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCGCGAGG GGCTGGGCGC CGCGTGCGAG GCCGCCCGGC CCCGGCTCTG CCGGCTCCTC 
    CGCAGGATCC TCCGGCCGCA GCCCGCCTGC CGCCTCCGCC GAGCCAGTCC AGTGACACAG
    GATGACCCCA AACGTCACAG CCCGGGCAAG CACGGGAATG AGTCTCCCCA GCCCCTCACG
    GGGACAGTAA AGACGTCTCC AGAATCCCTG AGCAAGCTGG ACGCGCCCCC GTCAGCCTGT
    CCACGGCATG TGAGGATCAA AAACTGGGGC AGCGGAGTGA CTTTCCAAGA CACGCTTCAC
    CAGAAGGCCA AAGGGGATCT CTCTTGCAAG TCCAAGTCTT GCCTGGCATC CATCATGAAC
    CCCAAAAGTT TGACCATAGG ACCCCGGGAC AAGCCAACCC CCCCAGACGA GCTTCTACCT
    CAAGCTATCG AATTTGTCAA CCAGTATTAC GGCTCCTTCA AAGAGGCAAA AATAGAGGAA
    CATCTGGCCA GGGTGGAAGC AGTAACAAAG GAGATAGAAA CAACAGGAAC CTACCAGCTG
    ACGGGAGATG AGCTCATCTT CGCCACCAAG CAGGCCTGGC GCAACGCCCC CCGCTGCATC
    GGAAGGATCC AGTGGTCGAA CCTGCAGGTC TTTGACGCCC GGAGCTGTTC CACGGCCCAG
    GAAATGTTCG AACACATCTG CAGACACGTG CGTTATGCCA CCAACAACGG CAACATCAGG
    TCGGCCATCA CTGTGTTCCC CCAGCGGAGC GATGGGAAGC ATGACTTCCG GGTCTGGAAC
    GCCCAGCTCA TCCGCTATGC CGGCTACCAG ATGCCAGATG GCAGCATCAG AGGGGACCCC
    GCCAACGTGG AGTTCACACA GCTGTGCATC GACCTGGGCT GGAAGCCCAA GTACGGCCGC
    TTCGACGTGT TGCCTCTAGT CCTGCAGGCT GACGGCCGTG ACCCAGAGCT CTTCGAAATC
    CCCCCTGACC TTGTTCTCGA GGTGCCCATG GAACATCCCA GGTACGAATG GTTCCGGGAG
    CTGGAGCTCA AGTGGTACGC CCTGCCAGCC GTGGCCAACA TGCTGCTTGA GGTGGGCGGC
    CTCGAGTTCC CAGGGTGCCC CTTCAACGGG TGGTACATGG GCACAGAAGT TGGAGTCCGG
    GACTTCTGCG ACGCCCAGCG ATACAACATC CTGGAGGAAG TGGGCAGAAG GATGGGCCTG
    GAAACGCACA AGGTGGCCTC ACTCTGGAAG GACCGGGCTG TCGTTGAGAT CAACGTCGCT
    GTGCTCCACA GTTTCCAGAA GCAGAACGTG ACCATCATGG ACCACCACTC CGCTGCAGAG
    TCCTTCATGA AGTACATGCA GAATGAGTAC CGCTCCCGAG GGGGCTGCCC AGCTGACTGG
    ATTTGGCTGG TCCCCCCGAT CTCTGGAAGC ATCACCCCCG TGTTCCACCA GGAGATGTTA
    AATTACGTCC TGTCCCCTTT CTACTACTAC CAGGTGGAGC CCTGGAAAAC CCACGTCTGG
    CAGGACGAGA GGCGGAGACC CCAGAGAAGA GAGATTCGAT TCAAAGTCTT GGTCAAAGCC
    GTGTTCTTCG CCTCGGTGCT GATGCATAAG ACCATGGCAT CCCGGGTCAG AGCCACGATC
    CTCTTTGCAA CAGAGACGGG GAGATCGGAA ACGCTGGCCC AGGACCTGGG GGCTTTGTTC
    AGCTGTGCCT TCAACCCCAA GGTTCTCTGC ATGGATCAGT ACCAGCTGAG CCATCTGGAG
    GAGGAGCAGC TGCTGCTGGT GGTGACCAGC ACTTTTGGCA ACGGAGACTC CCCTGGCAAC
    GGAGAGAAAC TGAAGAAGTC CCTCTTGATG CTGAAAGAAC TCACCAACAC GTTCCGGTAC
    GCCGTGTTTG GCCTTGGCTC CAGCATGTAC CCTCAGTTCT GCGCTTTTGC TCATGACATT
    GACCAGAAGT TGTCCCAGCT GGGGGCTTCT CAGCTTGTCC CAACCGGGGA AGGGGACGAA
    CTCAGCGGGC AGGAGGAGGC CTTTCGCAGC TGGGCCGTGC AAACCTTCAA GGCAGCCTGT
    GAGACATTCG ATGTCAGCGG CAAGCACCAC ATTGAGATCC CCAAGCTCTA CACTTCCAAT
    GTGACCTGGG ACCCGCAGCA CTACAGGCTC GTGCAGGACT CAGAGCCTCT GGACCTCAAC
    AAAGCCCTGA GCAGTATGCA CGCCAAGCAT GTGTTCACCA TGAGGCTCAA ATCGCAGCAG
    AATCTGCAGA GTCCGAAATC CAGCCGCACC ACCCTCCTGG TGGAACTCTC CTGTGAGGGC
    AGCCAAGCTC CAAGCTACCT GCCGGGAGAG CACCTCGGAG TTTTCCCATG CAACCAGCCG
    GCCCTGGTTC AAGGCATCCT TGAGCGAGTG GTGGATGGCC CTGCCCCGCA CCAGCCTGTG
    CGCCTGGAGA CCCTCTGTGA GAATGGCAGC TATTGGGTCA AGGATAAGCG GCTGCCGCCT
    TGCTCACTCA GCCAGGCTCT CACCTATTTC CTGGACATCA CCACCCCGCC AACCCAGCTG
    CTGCTCCGAA AGCTGGCCCA GCTGGCCACA GAAGAGGCTG AGAAGCAGAG GCTGGAGACC
    CTATGCCAGC CCTCAGATTA CAACAAGTGG AAGTTCACCA ACAGCCCCAC ATTTCTGGAG
    GTGCTGGAGG AGTTCCCGTC CCTGCGGGTG TCTGCCAGCT TCCTGCTCTC CCAGCTCCCT
    ATTCTGAAGC CCCGGTACTA CTCCATCAGC TCCTCCAGGG ACCTCACACC CACGGAGATC
    CACCTCACTG TGGCCGTGCT CACGTACCGC ACCCGAGATG GCCAAGGTCC CCTGCACCAT
    GGTGTCTGCA GCACGTGGCT CAGCAGCTTG AAGCCCCAAG ACCCGGTGCC CTGCTTTGTG
    CGAAGTGCCA GCGGCTTCCA GCTTCCCGAG GACCGCTCCC GTCCTTGCAT CCTCATTGGG
    CCCGGCACGG GCATCGCCCC CTTCCGCAGT TTCTGGCAGC AGCGGCTCCA TGAGGCTGAG
    CACAAAGGGC TCCAGGGCGG CCGCATGACC CTGGTTTTCG GGTGCCGCCG CCCAGAAGAG
    GACCACCTCT ACTGGGAGGA GATGCTGGAG ATGGCCCGCA AGGGGGTGCT GCACGAGGTG
    CACACCGCCT ATTCCCGCCT GCCTGACCAG CCCAAGGTCT ATGTTCAAGA CATCCTGCGG
    CAGCGGCTGG CGGGCGAGGT GCTTCGCGTG CTCCATGAGG AGCAAGGTCA CCTCTATGTC
    TGTGGGGATG TGCGCATGGC CCGGGACGTG GCCCGCACCC TGAAGCAGCT GATGGCTACT
    GCGCTGAGCC TGAACGAGGA ACAGGTGGAG GACTATTTCT TCCAGCTCAA GAACCAGAAG
    CGCTATCATG AAGACATCTT TGGTGCTGTA TTTCCCTATG AGGTGAAAAA GGATGGGGCA
    GCAGGGCTGC CCAGCAATCC CAGAGCTCCA GGCGCCCACA GAAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835414.1
    CDS109..3576
    Translation

    Target ORF information:

    RefSeq Version XM_024979646.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nitric oxide synthase 2 (NOS2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979646.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCGCGAGG GGCTGGGCGC CGCGTGCGAG GCCGCCCGGC CCCGGCTCTG CCGGCTCCTC 
    CGCAGGATCC TCCGGCCGCA GCCCGCCTGC CGCCTCCGCC GAGCCAGTCC AGTGACACAG
    GATGACCCCA AACGTCACAG CCCGGGCAAG CACGGGAATG AGTCTCCCCA GCCCCTCACG
    GGGACAGTAA AGACGTCTCC AGAATCCCTG AGCAAGCTGG ACGCGCCCCC GTCAGCCTGT
    CCACGGCATG TGAGGATCAA AAACTGGGGC AGCGGAGTGA CTTTCCAAGA CACGCTTCAC
    CAGAAGGCCA AAGGGGATCT CTCTTGCAAG TCCAAGTCTT GCCTGGCATC CATCATGAAC
    CCCAAAAGTT TGACCATAGG ACCCCGGGAC AAGCCAACCC CCCCAGACGA GCTTCTACCT
    CAAGCTATCG AATTTGTCAA CCAGTATTAC GGCTCCTTCA AAGAGGCAAA AATAGAGGAA
    CATCTGGCCA GGGTGGAAGC AGTAACAAAG GAGATAGAAA CAACAGGAAC CTACCAGCTG
    ACGGGAGATG AGCTCATCTT CGCCACCAAG CAGGCCTGGC GCAACGCCCC CCGCTGCATC
    GGAAGGATCC AGTGGTCGAA CCTGCAGGTC TTTGACGCCC GGAGCTGTTC CACGGCCCAG
    GAAATGTTCG AACACATCTG CAGACACGTG CGTTATGCCA CCAACAACGG CAACATCAGG
    TCGGCCATCA CTGTGTTCCC CCAGCGGAGC GATGGGAAGC ATGACTTCCG GGTCTGGAAC
    GCCCAGCTCA TCCGCTATGC CGGCTACCAG ATGCCAGATG GCAGCATCAG AGGGGACCCC
    GCCAACGTGG AGTTCACACA GCTGTGCATC GACCTGGGCT GGAAGCCCAA GTACGGCCGC
    TTCGACGTGT TGCCTCTAGT CCTGCAGGCT GACGGCCGTG ACCCAGAGCT CTTCGAAATC
    CCCCCTGACC TTGTTCTCGA GGTGCCCATG GAACATCCCA GGTACGAATG GTTCCGGGAG
    CTGGAGCTCA AGTGGTACGC CCTGCCAGCC GTGGCCAACA TGCTGCTTGA GGTGGGCGGC
    CTCGAGTTCC CAGGGTGCCC CTTCAACGGG TGGTACATGG GCACAGAAGT TGGAGTCCGG
    GACTTCTGCG ACGCCCAGCG ATACAACATC CTGGAGGAAG TGGGCAGAAG GATGGGCCTG
    GAAACGCACA AGGTGGCCTC ACTCTGGAAG GACCGGGCTG TCGTTGAGAT CAACGTCGCT
    GTGCTCCACA GTTTCCAGAA GCAGAACGTG ACCATCATGG ACCACCACTC CGCTGCAGAG
    TCCTTCATGA AGTACATGCA GAATGAGTAC CGCTCCCGAG GGGGCTGCCC AGCTGACTGG
    ATTTGGCTGG TCCCCCCGAT CTCTGGAAGC ATCACCCCCG TGTTCCACCA GGAGATGTTA
    AATTACGTCC TGTCCCCTTT CTACTACTAC CAGGTGGAGC CCTGGAAAAC CCACGTCTGG
    CAGGACGAGA GGCGGAGACC CCAGAGAAGA GAGATTCGAT TCAAAGTCTT GGTCAAAGCC
    GTGTTCTTCG CCTCGGTGCT GATGCATAAG ACCATGGCAT CCCGGGTCAG AGCCACGATC
    CTCTTTGCAA CAGAGACGGG GAGATCGGAA ACGCTGGCCC AGGACCTGGG GGCTTTGTTC
    AGCTGTGCCT TCAACCCCAA GGTTCTCTGC ATGGATCAGT ACCAGCTGAG CCATCTGGAG
    GAGGAGCAGC TGCTGCTGGT GGTGACCAGC ACTTTTGGCA ACGGAGACTC CCCTGGCAAC
    GGAGAGAAAC TGAAGAAGTC CCTCTTGATG CTGAAAGAAC TCACCAACAC GTTCCGGTAC
    GCCGTGTTTG GCCTTGGCTC CAGCATGTAC CCTCAGTTCT GCGCTTTTGC TCATGACATT
    GACCAGAAGT TGTCCCAGCT GGGGGCTTCT CAGCTTGTCC CAACCGGGGA AGGGGACGAA
    CTCAGCGGGC AGGAGGAGGC CTTTCGCAGC TGGGCCGTGC AAACCTTCAA GGCAGCCTGT
    GAGACATTCG ATGTCAGCGG CAAGCACCAC ATTGAGATCC CCAAGCTCTA CACTTCCAAT
    GTGACCTGGG ACCCGCAGCA CTACAGGCTC GTGCAGGACT CAGAGCCTCT GGACCTCAAC
    AAAGCCCTGA GCAGTATGCA CGCCAAGCAT GTGTTCACCA TGAGGCTCAA ATCGCAGCAG
    AATCTGCAGA GTCCGAAATC CAGCCGCACC ACCCTCCTGG TGGAACTCTC CTGTGAGGGC
    AGCCAAGCTC CAAGCTACCT GCCGGGAGAG CACCTCGGAG TTTTCCCATG CAACCAGCCG
    GCCCTGGTTC AAGGCATCCT TGAGCGAGTG GTGGATGGCC CTGCCCCGCA CCAGCCTGTG
    CGCCTGGAGA CCCTCTGTGA GAATGGCAGC TATTGGGTCA AGGATAAGCG GCTGCCGCCT
    TGCTCACTCA GCCAGGCTCT CACCTATTTC CTGGACATCA CCACCCCGCC AACCCAGCTG
    CTGCTCCGAA AGCTGGCCCA GCTGGCCACA GAAGAGGCTG AGAAGCAGAG GCTGGAGACC
    CTATGCCAGC CCTCAGATTA CAACAAGTGG AAGTTCACCA ACAGCCCCAC ATTTCTGGAG
    GTGCTGGAGG AGTTCCCGTC CCTGCGGGTG TCTGCCAGCT TCCTGCTCTC CCAGCTCCCT
    ATTCTGAAGC CCCGGTACTA CTCCATCAGC TCCTCCAGGG ACCTCACACC CACGGAGATC
    CACCTCACTG TGGCCGTGCT CACGTACCGC ACCCGAGATG GCCAAGGTCC CCTGCACCAT
    GGTGTCTGCA GCACGTGGCT CAGCAGCTTG AAGCCCCAAG ACCCGGTGCC CTGCTTTGTG
    CGAAGTGCCA GCGGCTTCCA GCTTCCCGAG GACCGCTCCC GTCCTTGCAT CCTCATTGGG
    CCCGGCACGG GCATCGCCCC CTTCCGCAGT TTCTGGCAGC AGCGGCTCCA TGAGGCTGAG
    CACAAAGGGC TCCAGGGCGG CCGCATGACC CTGGTTTTCG GGTGCCGCCG CCCAGAAGAG
    GACCACCTCT ACTGGGAGGA GATGCTGGAG ATGGCCCGCA AGGGGGTGCT GCACGAGGTG
    CACACCGCCT ATTCCCGCCT GCCTGACCAG CCCAAGGTCT ATGTTCAAGA CATCCTGCGG
    CAGCGGCTGG CGGGCGAGGT GCTTCGCGTG CTCCATGAGG AGCAAGGTCA CCTCTATGTC
    TGTGGGGATG TGCGCATGGC CCGGGACGTG GCCCGCACCC TGAAGCAGCT GATGGCTACT
    GCGCTGAGCC TGAACGAGGA ACAGGTGGAG GACTATTTCT TCCAGCTCAA GAACCAGAAG
    CGCTATCATG AAGACATCTT TGGTGCTGTA TTTCCCTATG AGGTGAAAAA GGATGGGGCA
    GCAGGGCTGC CCAGCAATCC CAGAGCTCCA GGCGCCCACA GAAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.