NOS2 cDNA ORF clone, Canis lupus familiaris(Dog)

The following NOS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe12200 NM_001313848.1
Latest version!
Canis lupus familiaris nitric oxide synthase 2 (NOS2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCe12200 XM_022422531.1
Latest version!
Canis lupus familiaris nitric oxide synthase 2 (NOS2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCe38270 XM_014116464.2
Latest version!
Canis lupus familiaris nitric oxide synthase 2 (NOS2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCe38271 XM_014116465.2
Latest version!
Canis lupus familiaris nitric oxide synthase 2 (NOS2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
View Old Accession Versions >>
OCe38270 XM_014116464.1 Canis lupus familiaris nitric oxide synthase 2, inducible (NOS2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCe38271 XM_014116465.1 Canis lupus familiaris nitric oxide synthase 2, inducible (NOS2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe12200
    Clone ID Related Accession (Same CDS sequence) XM_022422531.1 , NM_001313848.1
    Accession Version NM_001313848.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-13
    Organism Canis lupus familiaris(dog)
    Product nitric oxide synthase, inducible
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03006661.1 and AF032909.1. On Sep 4, 2015 this sequence version replaced XM_005624846.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA2240594 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCTGTC CCTGGCAGTT TCTGTTCAAG GCCAAATCCT ACCAGTACGA CTTGACCGAG 
    AAAACAGACA TTAACAACAA CGTGGGGAAA GCCAGCCAGG CCCCCTCCAG TCTAGTGACA
    CAGGATGACC CCAAGTGTCA CAGCCTCAGC AAGCACCGGG ATGAGTCCCC CCAGTCCCTC
    ACAGGGACAG TAAAGACGTC TCCAGAATCC ACAATCAAGC CAGCTGCGCC CCCACTGGCC
    TGCCCACGGC ATGTGAGGAT CAAAAACTGG GGCAACGGGA TGACCTTCCA AGACACACTT
    CACCACAAGG CCAAAGGGGA TCTCGCTTGC AAGTCCAAGT CTTGTCTGGG AGCCATCATG
    AACCCCAAAA GTTTGACCAG AGAACCCAGG GACAAGCCAA CCCCCCCAGA CGAGCTTCTA
    CCTCAAGCCA TTGAATTTGT CAACCAGTAT TACAGCTCCT TCAAAGAGGC AAAAATAGAG
    GAACATCTGG CCAGGGTGGA AGCAGTAACA AAGGAGATAG AAACAACAGG AACCTATCAG
    CTGACGGGGG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAACGC TCCCCGCTGC
    ATCGGGAGGA TCCAGTGGTC CAACCTCCAG GTCTTTGATG CCCGGAGCTG TTCCACTGCC
    AAGGAAATGT TTGAGCACAT CTGCAGGCAC CTGCGTTATG CCAGCAACAA TGGCAACATC
    AGGTCGGCCA TCACCGTGTT CCCCCAACGA ACTGATGGGA AGCATGACTT CCGAGTCTGG
    AACGCCCAGC TCATCCGCTA TGCTGGCTAC CAGATGCCCG ATGGCACCAT CCTGGGGGAC
    CCTGCCAGTG TGGAGTTCAC CCAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC
    CGCTTTGATG TGGTGCCTCT GGTGCTGCAG GCTGATGGCC AAGACCCTGA GTTCTTCGAA
    ATCCCACCTG ACCTTGTGCT CGAGGTACCC ATGGAACATC CCAAGTATGA GTGGTTCCGG
    GAGCTGGAGC TGAAGTGGTA CGCCCTGCCT GCAGTGGCCA ACATGCTGCT TGAGGTGGGT
    GGCCTTGAGT TCCCAGGGTG CCCCTTCAAC GGCTGGTACA TGGGCACGGA GATTGGGGTC
    CGGGACTTCT GTGATGTTCA GCGCTACAAC ATCCTGGAGG AAGTGGGCAG AAGGATGGGC
    CTGGAAACAC ACAAGCTGGC CTCACTCTGG AAGGACCGGG CTGTCATTGA GATCAATGTC
    GCTGTACTCC ACAGTTTCCA GAAGCAAAAT GTGACCATCA TGGATCACCA CTCAGCTGCA
    GAGTCCTTCA TGAAGTACAT GCAGAGCGAG TACCGCTCCC GAGGGGGCTG CCCTGCCGAC
    TGGATTTGGC TGGTCCCCCC AATTTCTGGG AGCATCACTC CTGTGTTCCA CCAGGAGATG
    TTAAATTATG TCCTGTCCCC TTTCTACTAC TACCAGGTGG AGGCCTGGAA AACCCACGTG
    TGGCAGGATG AGAAGCGGAG ACCCCAGAGG AGAAAGATTC AACTTAAAGT CTTGGTTAAA
    GCTGTGCTTT TTGCCTCCAT GCTGATGCGC AAGACCATGG CATCCCGAGT CAGAGTCACA
    ATCCTCTTTG CGACAGAGAC GGGAAAGTCA GAAACGCTAG CCCGGGACCT AGGGGCCTTG
    TTCAGCTGCG CCTTCCACCC CAAGGTTCTC TGCATGGACG AGTACAAGCT GAGCCACCTG
    GAGGAGGAGC AGCTACTGTT GGTGGTGACC AGCACGTTTG GGAACGGAGA CTCCCCTGGC
    AATGGAGAGA AACTAAAAAA ATCTCTCTTC ATGCTGAAAG AGCTTACCAA CAAGTTCAGG
    TATGCCGTGT TTGGCCTCGG CTCCAGCATG TACCCTCAGT TCTGTGCCTT TGCTCATGAC
    ATCGACCAGA AGCTATCGCA TCTGGGGGCC TCTCAGCTCG CCCCAATAGG GGAAGGGGAT
    GAGCTCAGTG GACAGGAGGA AGCCTTCCGC TGCTGGGCCG TGCAAACCTT CAAGGCAGCC
    TGTGAGACGT TTGATGTGCG AGGCAAACAC TGCATTCAGA TCCCCAGGCT CTACACTTCC
    AATGTGACCT GGGACCCACA CCAATACAGG CTCGTGCAGG ACTCGCAGCC TCTGGACCTC
    AACAAAGCCC TCAGCAGAAT GCATGCCAAG AACGTGTTCA CCCTGAGGCT CAAATCACAA
    CGGAATCTAC AGAGTCCAAA ATCCAGCCGC ACCACTCTCC TGGTCGAGCT CTCCTGTGAG
    GACAGCCAGG AGCTGAGCTA CCTGCCAGGA GAACATCTCG GAGTTTTCCC AGGCAACCAG
    CTGGCCCTGG TGCAAGGCAT CTTGGAGCGA GTAGTAGACA GCCCTGCACC CCACCAGCCT
    GTGCACCTGG AGACCCTCAG CGAGAGGGGA AGCTACTGGG TCAGAGACAA GCGGCTGCCT
    CCGTGCTCAC TCAGCCAGGC CCTCACCTAC TTCCTGGACA TCACCACTCC CCCGACCCAG
    CTGCTGCTCC GAAAGCTGGC ACAGCTGGCC ACAGAAGAGG CCGAGAGGCA GAGGTTGGAG
    ATCCTGTGCC AGCCCTCAGA ATACAACAAG TGGAAGTTAA CCAACAGTCC CACCTTCCTG
    GAGGTGCTGG AGGAGTTCCC ATCCCTGCGG GTGTCTGCTG GCTTCCTGCT CTCCCAGCTC
    CCTATTCTGA AACCGCGGTA CTACTCCATC AGCTCCTCCA GGGACTGCAC ACCCATGGAG
    GTCCACCTCA CGGTGGCTGT GCTCGTGTAC CCCACCCGAG ATGGCCAAGG TCCTTTGCAC
    CATGGTGTCT GTAGCACATG GCTCAGCAAC CTGAAGCCCC AAGACCCAGT ACCCTGCTTT
    GTACGAAGTG CCGGCAACTT CAAGCTCCCC GAGGATCCCT CCCGTCCTTG CATCCTCATT
    GGACCTGGCA CAGGCATCGC CCCCTTCCGC AGTTTCTGGC AGCAGCGGCT CCATGACATC
    AAGCACAAAG GGCTCCGAGG CAGCCGCATG ACCCTGGTGT TTGGGTGCCG CCGCCCAGAT
    GAGGACCACC TGTATCGGGA GGAGATGTTG GAGATGGCCC AGAGTGGGGT GCTGCATGAG
    GTGCACACAG CCTATTCTCG CCTGCCTGGC CAGCCCAAGG TCTATGTTCA AGACATCCTG
    CGGCAGCAGC TGGCCAGCCA GGTGCTCCGC ATGCTCCATG AGGAGCAGGG CCACCTTTAT
    GTCTGTGGGG ATGTGCGTAT GGCCCGGGAT GTGGCCCATA CCCTGAAGCA CCTGGTGGCT
    GCCAAGCTGA GCCTGAGTGA AGAGCAAGTT GAGGACTATT TTTTCCAGCT TAAGAGCCAG
    AAGCGCTATC ATGAAGATAT CTTTGGTGCT GTGTTTCCCT ATGAGGTGAA AAAAGATGGT
    GCAGCAAAAC AGCCCAGCGA TCCCAGAGTT CCAGCAGCCC ACGGAAGAAG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001300777.1
    CDS1..3474
    Translation

    Target ORF information:

    RefSeq Version NM_001313848.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nitric oxide synthase 2 (NOS2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001313848.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCTGTC CCTGGCAGTT TCTGTTCAAG GCCAAATCCT ACCAGTACGA CTTGACCGAG 
    AAAACAGACA TTAACAACAA CGTGGGGAAA GCCAGCCAGG CCCCCTCCAG TCTAGTGACA
    CAGGATGACC CCAAGTGTCA CAGCCTCAGC AAGCACCGGG ATGAGTCCCC CCAGTCCCTC
    ACAGGGACAG TAAAGACGTC TCCAGAATCC ACAATCAAGC CAGCTGCGCC CCCACTGGCC
    TGCCCACGGC ATGTGAGGAT CAAAAACTGG GGCAACGGGA TGACCTTCCA AGACACACTT
    CACCACAAGG CCAAAGGGGA TCTCGCTTGC AAGTCCAAGT CTTGTCTGGG AGCCATCATG
    AACCCCAAAA GTTTGACCAG AGAACCCAGG GACAAGCCAA CCCCCCCAGA CGAGCTTCTA
    CCTCAAGCCA TTGAATTTGT CAACCAGTAT TACAGCTCCT TCAAAGAGGC AAAAATAGAG
    GAACATCTGG CCAGGGTGGA AGCAGTAACA AAGGAGATAG AAACAACAGG AACCTATCAG
    CTGACGGGGG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAACGC TCCCCGCTGC
    ATCGGGAGGA TCCAGTGGTC CAACCTCCAG GTCTTTGATG CCCGGAGCTG TTCCACTGCC
    AAGGAAATGT TTGAGCACAT CTGCAGGCAC CTGCGTTATG CCAGCAACAA TGGCAACATC
    AGGTCGGCCA TCACCGTGTT CCCCCAACGA ACTGATGGGA AGCATGACTT CCGAGTCTGG
    AACGCCCAGC TCATCCGCTA TGCTGGCTAC CAGATGCCCG ATGGCACCAT CCTGGGGGAC
    CCTGCCAGTG TGGAGTTCAC CCAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC
    CGCTTTGATG TGGTGCCTCT GGTGCTGCAG GCTGATGGCC AAGACCCTGA GTTCTTCGAA
    ATCCCACCTG ACCTTGTGCT CGAGGTACCC ATGGAACATC CCAAGTATGA GTGGTTCCGG
    GAGCTGGAGC TGAAGTGGTA CGCCCTGCCT GCAGTGGCCA ACATGCTGCT TGAGGTGGGT
    GGCCTTGAGT TCCCAGGGTG CCCCTTCAAC GGCTGGTACA TGGGCACGGA GATTGGGGTC
    CGGGACTTCT GTGATGTTCA GCGCTACAAC ATCCTGGAGG AAGTGGGCAG AAGGATGGGC
    CTGGAAACAC ACAAGCTGGC CTCACTCTGG AAGGACCGGG CTGTCATTGA GATCAATGTC
    GCTGTACTCC ACAGTTTCCA GAAGCAAAAT GTGACCATCA TGGATCACCA CTCAGCTGCA
    GAGTCCTTCA TGAAGTACAT GCAGAGCGAG TACCGCTCCC GAGGGGGCTG CCCTGCCGAC
    TGGATTTGGC TGGTCCCCCC AATTTCTGGG AGCATCACTC CTGTGTTCCA CCAGGAGATG
    TTAAATTATG TCCTGTCCCC TTTCTACTAC TACCAGGTGG AGGCCTGGAA AACCCACGTG
    TGGCAGGATG AGAAGCGGAG ACCCCAGAGG AGAAAGATTC AACTTAAAGT CTTGGTTAAA
    GCTGTGCTTT TTGCCTCCAT GCTGATGCGC AAGACCATGG CATCCCGAGT CAGAGTCACA
    ATCCTCTTTG CGACAGAGAC GGGAAAGTCA GAAACGCTAG CCCGGGACCT AGGGGCCTTG
    TTCAGCTGCG CCTTCCACCC CAAGGTTCTC TGCATGGACG AGTACAAGCT GAGCCACCTG
    GAGGAGGAGC AGCTACTGTT GGTGGTGACC AGCACGTTTG GGAACGGAGA CTCCCCTGGC
    AATGGAGAGA AACTAAAAAA ATCTCTCTTC ATGCTGAAAG AGCTTACCAA CAAGTTCAGG
    TATGCCGTGT TTGGCCTCGG CTCCAGCATG TACCCTCAGT TCTGTGCCTT TGCTCATGAC
    ATCGACCAGA AGCTATCGCA TCTGGGGGCC TCTCAGCTCG CCCCAATAGG GGAAGGGGAT
    GAGCTCAGTG GACAGGAGGA AGCCTTCCGC TGCTGGGCCG TGCAAACCTT CAAGGCAGCC
    TGTGAGACGT TTGATGTGCG AGGCAAACAC TGCATTCAGA TCCCCAGGCT CTACACTTCC
    AATGTGACCT GGGACCCACA CCAATACAGG CTCGTGCAGG ACTCGCAGCC TCTGGACCTC
    AACAAAGCCC TCAGCAGAAT GCATGCCAAG AACGTGTTCA CCCTGAGGCT CAAATCACAA
    CGGAATCTAC AGAGTCCAAA ATCCAGCCGC ACCACTCTCC TGGTCGAGCT CTCCTGTGAG
    GACAGCCAGG AGCTGAGCTA CCTGCCAGGA GAACATCTCG GAGTTTTCCC AGGCAACCAG
    CTGGCCCTGG TGCAAGGCAT CTTGGAGCGA GTAGTAGACA GCCCTGCACC CCACCAGCCT
    GTGCACCTGG AGACCCTCAG CGAGAGGGGA AGCTACTGGG TCAGAGACAA GCGGCTGCCT
    CCGTGCTCAC TCAGCCAGGC CCTCACCTAC TTCCTGGACA TCACCACTCC CCCGACCCAG
    CTGCTGCTCC GAAAGCTGGC ACAGCTGGCC ACAGAAGAGG CCGAGAGGCA GAGGTTGGAG
    ATCCTGTGCC AGCCCTCAGA ATACAACAAG TGGAAGTTAA CCAACAGTCC CACCTTCCTG
    GAGGTGCTGG AGGAGTTCCC ATCCCTGCGG GTGTCTGCTG GCTTCCTGCT CTCCCAGCTC
    CCTATTCTGA AACCGCGGTA CTACTCCATC AGCTCCTCCA GGGACTGCAC ACCCATGGAG
    GTCCACCTCA CGGTGGCTGT GCTCGTGTAC CCCACCCGAG ATGGCCAAGG TCCTTTGCAC
    CATGGTGTCT GTAGCACATG GCTCAGCAAC CTGAAGCCCC AAGACCCAGT ACCCTGCTTT
    GTACGAAGTG CCGGCAACTT CAAGCTCCCC GAGGATCCCT CCCGTCCTTG CATCCTCATT
    GGACCTGGCA CAGGCATCGC CCCCTTCCGC AGTTTCTGGC AGCAGCGGCT CCATGACATC
    AAGCACAAAG GGCTCCGAGG CAGCCGCATG ACCCTGGTGT TTGGGTGCCG CCGCCCAGAT
    GAGGACCACC TGTATCGGGA GGAGATGTTG GAGATGGCCC AGAGTGGGGT GCTGCATGAG
    GTGCACACAG CCTATTCTCG CCTGCCTGGC CAGCCCAAGG TCTATGTTCA AGACATCCTG
    CGGCAGCAGC TGGCCAGCCA GGTGCTCCGC ATGCTCCATG AGGAGCAGGG CCACCTTTAT
    GTCTGTGGGG ATGTGCGTAT GGCCCGGGAT GTGGCCCATA CCCTGAAGCA CCTGGTGGCT
    GCCAAGCTGA GCCTGAGTGA AGAGCAAGTT GAGGACTATT TTTTCCAGCT TAAGAGCCAG
    AAGCGCTATC ATGAAGATAT CTTTGGTGCT GTGTTTCCCT ATGAGGTGAA AAAAGATGGT
    GCAGCAAAAC AGCCCAGCGA TCCCAGAGTT CCAGCAGCCC ACGGAAGAAG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12200
    Clone ID Related Accession (Same CDS sequence) XM_022422531.1 , NM_001313848.1
    Accession Version XM_022422531.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nitric oxide synthase, inducible isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCTGTC CCTGGCAGTT TCTGTTCAAG GCCAAATCCT ACCAGTACGA CTTGACCGAG 
    AAAACAGACA TTAACAACAA CGTGGGGAAA GCCAGCCAGG CCCCCTCCAG TCTAGTGACA
    CAGGATGACC CCAAGTGTCA CAGCCTCAGC AAGCACCGGG ATGAGTCCCC CCAGTCCCTC
    ACAGGGACAG TAAAGACGTC TCCAGAATCC ACAATCAAGC CAGCTGCGCC CCCACTGGCC
    TGCCCACGGC ATGTGAGGAT CAAAAACTGG GGCAACGGGA TGACCTTCCA AGACACACTT
    CACCACAAGG CCAAAGGGGA TCTCGCTTGC AAGTCCAAGT CTTGTCTGGG AGCCATCATG
    AACCCCAAAA GTTTGACCAG AGAACCCAGG GACAAGCCAA CCCCCCCAGA CGAGCTTCTA
    CCTCAAGCCA TTGAATTTGT CAACCAGTAT TACAGCTCCT TCAAAGAGGC AAAAATAGAG
    GAACATCTGG CCAGGGTGGA AGCAGTAACA AAGGAGATAG AAACAACAGG AACCTATCAG
    CTGACGGGGG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAACGC TCCCCGCTGC
    ATCGGGAGGA TCCAGTGGTC CAACCTCCAG GTCTTTGATG CCCGGAGCTG TTCCACTGCC
    AAGGAAATGT TTGAGCACAT CTGCAGGCAC CTGCGTTATG CCAGCAACAA TGGCAACATC
    AGGTCGGCCA TCACCGTGTT CCCCCAACGA ACTGATGGGA AGCATGACTT CCGAGTCTGG
    AACGCCCAGC TCATCCGCTA TGCTGGCTAC CAGATGCCCG ATGGCACCAT CCTGGGGGAC
    CCTGCCAGTG TGGAGTTCAC CCAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC
    CGCTTTGATG TGGTGCCTCT GGTGCTGCAG GCTGATGGCC AAGACCCTGA GTTCTTCGAA
    ATCCCACCTG ACCTTGTGCT CGAGGTACCC ATGGAACATC CCAAGTATGA GTGGTTCCGG
    GAGCTGGAGC TGAAGTGGTA CGCCCTGCCT GCAGTGGCCA ACATGCTGCT TGAGGTGGGT
    GGCCTTGAGT TCCCAGGGTG CCCCTTCAAC GGCTGGTACA TGGGCACGGA GATTGGGGTC
    CGGGACTTCT GTGATGTTCA GCGCTACAAC ATCCTGGAGG AAGTGGGCAG AAGGATGGGC
    CTGGAAACAC ACAAGCTGGC CTCACTCTGG AAGGACCGGG CTGTCATTGA GATCAATGTC
    GCTGTACTCC ACAGTTTCCA GAAGCAAAAT GTGACCATCA TGGATCACCA CTCAGCTGCA
    GAGTCCTTCA TGAAGTACAT GCAGAGCGAG TACCGCTCCC GAGGGGGCTG CCCTGCCGAC
    TGGATTTGGC TGGTCCCCCC AATTTCTGGG AGCATCACTC CTGTGTTCCA CCAGGAGATG
    TTAAATTATG TCCTGTCCCC TTTCTACTAC TACCAGGTGG AGGCCTGGAA AACCCACGTG
    TGGCAGGATG AGAAGCGGAG ACCCCAGAGG AGAAAGATTC AACTTAAAGT CTTGGTTAAA
    GCTGTGCTTT TTGCCTCCAT GCTGATGCGC AAGACCATGG CATCCCGAGT CAGAGTCACA
    ATCCTCTTTG CGACAGAGAC GGGAAAGTCA GAAACGCTAG CCCGGGACCT AGGGGCCTTG
    TTCAGCTGCG CCTTCCACCC CAAGGTTCTC TGCATGGACG AGTACAAGCT GAGCCACCTG
    GAGGAGGAGC AGCTACTGTT GGTGGTGACC AGCACGTTTG GGAACGGAGA CTCCCCTGGC
    AATGGAGAGA AACTAAAAAA ATCTCTCTTC ATGCTGAAAG AGCTTACCAA CAAGTTCAGG
    TATGCCGTGT TTGGCCTCGG CTCCAGCATG TACCCTCAGT TCTGTGCCTT TGCTCATGAC
    ATCGACCAGA AGCTATCGCA TCTGGGGGCC TCTCAGCTCG CCCCAATAGG GGAAGGGGAT
    GAGCTCAGTG GACAGGAGGA AGCCTTCCGC TGCTGGGCCG TGCAAACCTT CAAGGCAGCC
    TGTGAGACGT TTGATGTGCG AGGCAAACAC TGCATTCAGA TCCCCAGGCT CTACACTTCC
    AATGTGACCT GGGACCCACA CCAATACAGG CTCGTGCAGG ACTCGCAGCC TCTGGACCTC
    AACAAAGCCC TCAGCAGAAT GCATGCCAAG AACGTGTTCA CCCTGAGGCT CAAATCACAA
    CGGAATCTAC AGAGTCCAAA ATCCAGCCGC ACCACTCTCC TGGTCGAGCT CTCCTGTGAG
    GACAGCCAGG AGCTGAGCTA CCTGCCAGGA GAACATCTCG GAGTTTTCCC AGGCAACCAG
    CTGGCCCTGG TGCAAGGCAT CTTGGAGCGA GTAGTAGACA GCCCTGCACC CCACCAGCCT
    GTGCACCTGG AGACCCTCAG CGAGAGGGGA AGCTACTGGG TCAGAGACAA GCGGCTGCCT
    CCGTGCTCAC TCAGCCAGGC CCTCACCTAC TTCCTGGACA TCACCACTCC CCCGACCCAG
    CTGCTGCTCC GAAAGCTGGC ACAGCTGGCC ACAGAAGAGG CCGAGAGGCA GAGGTTGGAG
    ATCCTGTGCC AGCCCTCAGA ATACAACAAG TGGAAGTTAA CCAACAGTCC CACCTTCCTG
    GAGGTGCTGG AGGAGTTCCC ATCCCTGCGG GTGTCTGCTG GCTTCCTGCT CTCCCAGCTC
    CCTATTCTGA AACCGCGGTA CTACTCCATC AGCTCCTCCA GGGACTGCAC ACCCATGGAG
    GTCCACCTCA CGGTGGCTGT GCTCGTGTAC CCCACCCGAG ATGGCCAAGG TCCTTTGCAC
    CATGGTGTCT GTAGCACATG GCTCAGCAAC CTGAAGCCCC AAGACCCAGT ACCCTGCTTT
    GTACGAAGTG CCGGCAACTT CAAGCTCCCC GAGGATCCCT CCCGTCCTTG CATCCTCATT
    GGACCTGGCA CAGGCATCGC CCCCTTCCGC AGTTTCTGGC AGCAGCGGCT CCATGACATC
    AAGCACAAAG GGCTCCGAGG CAGCCGCATG ACCCTGGTGT TTGGGTGCCG CCGCCCAGAT
    GAGGACCACC TGTATCGGGA GGAGATGTTG GAGATGGCCC AGAGTGGGGT GCTGCATGAG
    GTGCACACAG CCTATTCTCG CCTGCCTGGC CAGCCCAAGG TCTATGTTCA AGACATCCTG
    CGGCAGCAGC TGGCCAGCCA GGTGCTCCGC ATGCTCCATG AGGAGCAGGG CCACCTTTAT
    GTCTGTGGGG ATGTGCGTAT GGCCCGGGAT GTGGCCCATA CCCTGAAGCA CCTGGTGGCT
    GCCAAGCTGA GCCTGAGTGA AGAGCAAGTT GAGGACTATT TTTTCCAGCT TAAGAGCCAG
    AAGCGCTATC ATGAAGATAT CTTTGGTGCT GTGTTTCCCT ATGAGGTGAA AAAAGATGGT
    GCAGCAAAAC AGCCCAGCGA TCCCAGAGTT CCAGCAGCCC ACGGAAGAAG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022278239.1
    CDS248..3721
    Translation

    Target ORF information:

    RefSeq Version XM_022422531.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nitric oxide synthase 2 (NOS2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022422531.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCTGTC CCTGGCAGTT TCTGTTCAAG GCCAAATCCT ACCAGTACGA CTTGACCGAG 
    AAAACAGACA TTAACAACAA CGTGGGGAAA GCCAGCCAGG CCCCCTCCAG TCTAGTGACA
    CAGGATGACC CCAAGTGTCA CAGCCTCAGC AAGCACCGGG ATGAGTCCCC CCAGTCCCTC
    ACAGGGACAG TAAAGACGTC TCCAGAATCC ACAATCAAGC CAGCTGCGCC CCCACTGGCC
    TGCCCACGGC ATGTGAGGAT CAAAAACTGG GGCAACGGGA TGACCTTCCA AGACACACTT
    CACCACAAGG CCAAAGGGGA TCTCGCTTGC AAGTCCAAGT CTTGTCTGGG AGCCATCATG
    AACCCCAAAA GTTTGACCAG AGAACCCAGG GACAAGCCAA CCCCCCCAGA CGAGCTTCTA
    CCTCAAGCCA TTGAATTTGT CAACCAGTAT TACAGCTCCT TCAAAGAGGC AAAAATAGAG
    GAACATCTGG CCAGGGTGGA AGCAGTAACA AAGGAGATAG AAACAACAGG AACCTATCAG
    CTGACGGGGG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAACGC TCCCCGCTGC
    ATCGGGAGGA TCCAGTGGTC CAACCTCCAG GTCTTTGATG CCCGGAGCTG TTCCACTGCC
    AAGGAAATGT TTGAGCACAT CTGCAGGCAC CTGCGTTATG CCAGCAACAA TGGCAACATC
    AGGTCGGCCA TCACCGTGTT CCCCCAACGA ACTGATGGGA AGCATGACTT CCGAGTCTGG
    AACGCCCAGC TCATCCGCTA TGCTGGCTAC CAGATGCCCG ATGGCACCAT CCTGGGGGAC
    CCTGCCAGTG TGGAGTTCAC CCAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC
    CGCTTTGATG TGGTGCCTCT GGTGCTGCAG GCTGATGGCC AAGACCCTGA GTTCTTCGAA
    ATCCCACCTG ACCTTGTGCT CGAGGTACCC ATGGAACATC CCAAGTATGA GTGGTTCCGG
    GAGCTGGAGC TGAAGTGGTA CGCCCTGCCT GCAGTGGCCA ACATGCTGCT TGAGGTGGGT
    GGCCTTGAGT TCCCAGGGTG CCCCTTCAAC GGCTGGTACA TGGGCACGGA GATTGGGGTC
    CGGGACTTCT GTGATGTTCA GCGCTACAAC ATCCTGGAGG AAGTGGGCAG AAGGATGGGC
    CTGGAAACAC ACAAGCTGGC CTCACTCTGG AAGGACCGGG CTGTCATTGA GATCAATGTC
    GCTGTACTCC ACAGTTTCCA GAAGCAAAAT GTGACCATCA TGGATCACCA CTCAGCTGCA
    GAGTCCTTCA TGAAGTACAT GCAGAGCGAG TACCGCTCCC GAGGGGGCTG CCCTGCCGAC
    TGGATTTGGC TGGTCCCCCC AATTTCTGGG AGCATCACTC CTGTGTTCCA CCAGGAGATG
    TTAAATTATG TCCTGTCCCC TTTCTACTAC TACCAGGTGG AGGCCTGGAA AACCCACGTG
    TGGCAGGATG AGAAGCGGAG ACCCCAGAGG AGAAAGATTC AACTTAAAGT CTTGGTTAAA
    GCTGTGCTTT TTGCCTCCAT GCTGATGCGC AAGACCATGG CATCCCGAGT CAGAGTCACA
    ATCCTCTTTG CGACAGAGAC GGGAAAGTCA GAAACGCTAG CCCGGGACCT AGGGGCCTTG
    TTCAGCTGCG CCTTCCACCC CAAGGTTCTC TGCATGGACG AGTACAAGCT GAGCCACCTG
    GAGGAGGAGC AGCTACTGTT GGTGGTGACC AGCACGTTTG GGAACGGAGA CTCCCCTGGC
    AATGGAGAGA AACTAAAAAA ATCTCTCTTC ATGCTGAAAG AGCTTACCAA CAAGTTCAGG
    TATGCCGTGT TTGGCCTCGG CTCCAGCATG TACCCTCAGT TCTGTGCCTT TGCTCATGAC
    ATCGACCAGA AGCTATCGCA TCTGGGGGCC TCTCAGCTCG CCCCAATAGG GGAAGGGGAT
    GAGCTCAGTG GACAGGAGGA AGCCTTCCGC TGCTGGGCCG TGCAAACCTT CAAGGCAGCC
    TGTGAGACGT TTGATGTGCG AGGCAAACAC TGCATTCAGA TCCCCAGGCT CTACACTTCC
    AATGTGACCT GGGACCCACA CCAATACAGG CTCGTGCAGG ACTCGCAGCC TCTGGACCTC
    AACAAAGCCC TCAGCAGAAT GCATGCCAAG AACGTGTTCA CCCTGAGGCT CAAATCACAA
    CGGAATCTAC AGAGTCCAAA ATCCAGCCGC ACCACTCTCC TGGTCGAGCT CTCCTGTGAG
    GACAGCCAGG AGCTGAGCTA CCTGCCAGGA GAACATCTCG GAGTTTTCCC AGGCAACCAG
    CTGGCCCTGG TGCAAGGCAT CTTGGAGCGA GTAGTAGACA GCCCTGCACC CCACCAGCCT
    GTGCACCTGG AGACCCTCAG CGAGAGGGGA AGCTACTGGG TCAGAGACAA GCGGCTGCCT
    CCGTGCTCAC TCAGCCAGGC CCTCACCTAC TTCCTGGACA TCACCACTCC CCCGACCCAG
    CTGCTGCTCC GAAAGCTGGC ACAGCTGGCC ACAGAAGAGG CCGAGAGGCA GAGGTTGGAG
    ATCCTGTGCC AGCCCTCAGA ATACAACAAG TGGAAGTTAA CCAACAGTCC CACCTTCCTG
    GAGGTGCTGG AGGAGTTCCC ATCCCTGCGG GTGTCTGCTG GCTTCCTGCT CTCCCAGCTC
    CCTATTCTGA AACCGCGGTA CTACTCCATC AGCTCCTCCA GGGACTGCAC ACCCATGGAG
    GTCCACCTCA CGGTGGCTGT GCTCGTGTAC CCCACCCGAG ATGGCCAAGG TCCTTTGCAC
    CATGGTGTCT GTAGCACATG GCTCAGCAAC CTGAAGCCCC AAGACCCAGT ACCCTGCTTT
    GTACGAAGTG CCGGCAACTT CAAGCTCCCC GAGGATCCCT CCCGTCCTTG CATCCTCATT
    GGACCTGGCA CAGGCATCGC CCCCTTCCGC AGTTTCTGGC AGCAGCGGCT CCATGACATC
    AAGCACAAAG GGCTCCGAGG CAGCCGCATG ACCCTGGTGT TTGGGTGCCG CCGCCCAGAT
    GAGGACCACC TGTATCGGGA GGAGATGTTG GAGATGGCCC AGAGTGGGGT GCTGCATGAG
    GTGCACACAG CCTATTCTCG CCTGCCTGGC CAGCCCAAGG TCTATGTTCA AGACATCCTG
    CGGCAGCAGC TGGCCAGCCA GGTGCTCCGC ATGCTCCATG AGGAGCAGGG CCACCTTTAT
    GTCTGTGGGG ATGTGCGTAT GGCCCGGGAT GTGGCCCATA CCCTGAAGCA CCTGGTGGCT
    GCCAAGCTGA GCCTGAGTGA AGAGCAAGTT GAGGACTATT TTTTCCAGCT TAAGAGCCAG
    AAGCGCTATC ATGAAGATAT CTTTGGTGCT GTGTTTCCCT ATGAGGTGAA AAAAGATGGT
    GCAGCAAAAC AGCCCAGCGA TCCCAGAGTT CCAGCAGCCC ACGGAAGAAG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe38270
    Clone ID Related Accession (Same CDS sequence) XM_014116464.1 , XM_014116464.2
    Accession Version XM_014116464.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nitric oxide synthase, inducible isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCGCGAGG GGCTGGGCGC TGCGTGCGAG GCCGCCCGGC CCCGGCTCTG CCGCCTCCTC 
    CGCGGGATCC TGCGGCCGCA GCCCGCCTGC AGGCCCCCGC GAGCCAGTCT AGTGACACAG
    GATGACCCCA AGTGTCACAG CCTCAGCAAG CACCGGGATG AGTCCCCCCA GTCCCTCACA
    GGGACAGTAA AGACGTCTCC AGAATCCACA ATCAAGCCAG CTGCGCCCCC ACTGGCCTGC
    CCACGGCATG TGAGGATCAA AAACTGGGGC AACGGGATGA CCTTCCAAGA CACACTTCAC
    CACAAGGCCA AAGGGGATCT CGCTTGCAAG TCCAAGTCTT GTCTGGGAGC CATCATGAAC
    CCCAAAAGTT TGACCAGAGA ACCCAGGGAC AAGCCAACCC CCCCAGACGA GCTTCTACCT
    CAAGCCATTG AATTTGTCAA CCAGTATTAC AGCTCCTTCA AAGAGGCAAA AATAGAGGAA
    CATCTGGCCA GGGTGGAAGC AGTAACAAAG GAGATAGAAA CAACAGGAAC CTATCAGCTG
    ACGGGGGATG AGCTCATCTT CGCCACCAAG CAGGCCTGGC GCAACGCTCC CCGCTGCATC
    GGGAGGATCC AGTGGTCCAA CCTCCAGGTC TTTGATGCCC GGAGCTGTTC CACTGCCAAG
    GAAATGTTTG AGCACATCTG CAGGCACCTG CGTTATGCCA GCAACAATGG CAACATCAGG
    TCGGCCATCA CCGTGTTCCC CCAACGAACT GATGGGAAGC ATGACTTCCG AGTCTGGAAC
    GCCCAGCTCA TCCGCTATGC TGGCTACCAG ATGCCCGATG GCACCATCCT GGGGGACCCT
    GCCAGTGTGG AGTTCACCCA GCTGTGCATC GACCTGGGCT GGAAGCCCAA GTACGGCCGC
    TTTGATGTGG TGCCTCTGGT GCTGCAGGCT GATGGCCAAG ACCCTGAGTT CTTCGAAATC
    CCACCTGACC TTGTGCTCGA GGTACCCATG GAACATCCCA AGTATGAGTG GTTCCGGGAG
    CTGGAGCTGA AGTGGTACGC CCTGCCTGCA GTGGCCAACA TGCTGCTTGA GGTGGGTGGC
    CTTGAGTTCC CAGGGTGCCC CTTCAACGGC TGGTACATGG GCACGGAGAT TGGGGTCCGG
    GACTTCTGTG ATGTTCAGCG CTACAACATC CTGGAGGAAG TGGGCAGAAG GATGGGCCTG
    GAAACACACA AGCTGGCCTC ACTCTGGAAG GACCGGGCTG TCATTGAGAT CAATGTCGCT
    GTACTCCACA GTTTCCAGAA GCAAAATGTG ACCATCATGG ATCACCACTC AGCTGCAGAG
    TCCTTCATGA AGTACATGCA GAGCGAGTAC CGCTCCCGAG GGGGCTGCCC TGCCGACTGG
    ATTTGGCTGG TCCCCCCAAT TTCTGGGAGC ATCACTCCTG TGTTCCACCA GGAGATGTTA
    AATTATGTCC TGTCCCCTTT CTACTACTAC CAGGTGGAGG CCTGGAAAAC CCACGTGTGG
    CAGGATGAGA AGCGGAGACC CCAGAGGAGA AAGATTCAAC TTAAAGTCTT GGTTAAAGCT
    GTGCTTTTTG CCTCCATGCT GATGCGCAAG ACCATGGCAT CCCGAGTCAG AGTCACAATC
    CTCTTTGCGA CAGAGACGGG AAAGTCAGAA ACGCTAGCCC GGGACCTAGG GGCCTTGTTC
    AGCTGCGCCT TCCACCCCAA GGTTCTCTGC ATGGACGAGT ACAAGCTGAG CCACCTGGAG
    GAGGAGCAGC TACTGTTGGT GGTGACCAGC ACGTTTGGGA ACGGAGACTC CCCTGGCAAT
    GGAGAGAAAC TAAAAAAATC TCTCTTCATG CTGAAAGAGC TTACCAACAA GTTCAGGTAT
    GCCGTGTTTG GCCTCGGCTC CAGCATGTAC CCTCAGTTCT GTGCCTTTGC TCATGACATC
    GACCAGAAGC TATCGCATCT GGGGGCCTCT CAGCTCGCCC CAATAGGGGA AGGGGATGAG
    CTCAGTGGAC AGGAGGAAGC CTTCCGCTGC TGGGCCGTGC AAACCTTCAA GGCAGCCTGT
    GAGACGTTTG ATGTGCGAGG CAAACACTGC ATTCAGATCC CCAGGCTCTA CACTTCCAAT
    GTGACCTGGG ACCCACACCA ATACAGGCTC GTGCAGGACT CGCAGCCTCT GGACCTCAAC
    AAAGCCCTCA GCAGAATGCA TGCCAAGAAC GTGTTCACCC TGAGGCTCAA ATCACAACGG
    AATCTACAGA GTCCAAAATC CAGCCGCACC ACTCTCCTGG TCGAGCTCTC CTGTGAGGAC
    AGCCAGGAGC TGAGCTACCT GCCAGGAGAA CATCTCGGAG TTTTCCCAGG CAACCAGCTG
    GCCCTGGTGC AAGGCATCTT GGAGCGAGTA GTAGACAGCC CTGCACCCCA CCAGCCTGTG
    CACCTGGAGA CCCTCAGCGA GAGGGGAAGC TACTGGGTCA GAGACAAGCG GCTGCCTCCG
    TGCTCACTCA GCCAGGCCCT CACCTACTTC CTGGACATCA CCACTCCCCC GACCCAGCTG
    CTGCTCCGAA AGCTGGCACA GCTGGCCACA GAAGAGGCCG AGAGGCAGAG GTTGGAGATC
    CTGTGCCAGC CCTCAGAATA CAACAAGTGG AAGTTAACCA ACAGTCCCAC CTTCCTGGAG
    GTGCTGGAGG AGTTCCCATC CCTGCGGGTG TCTGCTGGCT TCCTGCTCTC CCAGCTCCCT
    ATTCTGAAAC CGCGGTACTA CTCCATCAGC TCCTCCAGGG ACTGCACACC CATGGAGGTC
    CACCTCACGG TGGCTGTGCT CGTGTACCCC ACCCGAGATG GCCAAGGTCC TTTGCACCAT
    GGTGTCTGTA GCACATGGCT CAGCAACCTG AAGCCCCAAG ACCCAGTACC CTGCTTTGTA
    CGAAGTGCCG GCAACTTCAA GCTCCCCGAG GATCCCTCCC GTCCTTGCAT CCTCATTGGA
    CCTGGCACAG GCATCGCCCC CTTCCGCAGT TTCTGGCAGC AGCGGCTCCA TGACATCAAG
    CACAAAGGGC TCCGAGGCAG CCGCATGACC CTGGTGTTTG GGTGCCGCCG CCCAGATGAG
    GACCACCTGT ATCGGGAGGA GATGTTGGAG ATGGCCCAGA GTGGGGTGCT GCATGAGGTG
    CACACAGCCT ATTCTCGCCT GCCTGGCCAG CCCAAGGTCT ATGTTCAAGA CATCCTGCGG
    CAGCAGCTGG CCAGCCAGGT GCTCCGCATG CTCCATGAGG AGCAGGGCCA CCTTTATGTC
    TGTGGGGATG TGCGTATGGC CCGGGATGTG GCCCATACCC TGAAGCACCT GGTGGCTGCC
    AAGCTGAGCC TGAGTGAAGA GCAAGTTGAG GACTATTTTT TCCAGCTTAA GAGCCAGAAG
    CGCTATCATG AAGATATCTT TGGTGCTGTG TTTCCCTATG AGGTGAAAAA AGATGGTGCA
    GCAAAACAGC CCAGCGATCC CAGAGTTCCA GCAGCCCACG GAAGAAGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013971939.1
    CDS38..3508
    Translation

    Target ORF information:

    RefSeq Version XM_014116464.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nitric oxide synthase 2, inducible (NOS2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014116464.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCGCGAGG GGCTGGGCGC TGCGTGCGAG GCCGCCCGGC CCCGGCTCTG CCGCCTCCTC 
    CGCGGGATCC TGCGGCCGCA GCCCGCCTGC AGGCCCCCGC GAGCCAGTCT AGTGACACAG
    GATGACCCCA AGTGTCACAG CCTCAGCAAG CACCGGGATG AGTCCCCCCA GTCCCTCACA
    GGGACAGTAA AGACGTCTCC AGAATCCACA ATCAAGCCAG CTGCGCCCCC ACTGGCCTGC
    CCACGGCATG TGAGGATCAA AAACTGGGGC AACGGGATGA CCTTCCAAGA CACACTTCAC
    CACAAGGCCA AAGGGGATCT CGCTTGCAAG TCCAAGTCTT GTCTGGGAGC CATCATGAAC
    CCCAAAAGTT TGACCAGAGA ACCCAGGGAC AAGCCAACCC CCCCAGACGA GCTTCTACCT
    CAAGCCATTG AATTTGTCAA CCAGTATTAC AGCTCCTTCA AAGAGGCAAA AATAGAGGAA
    CATCTGGCCA GGGTGGAAGC AGTAACAAAG GAGATAGAAA CAACAGGAAC CTATCAGCTG
    ACGGGGGATG AGCTCATCTT CGCCACCAAG CAGGCCTGGC GCAACGCTCC CCGCTGCATC
    GGGAGGATCC AGTGGTCCAA CCTCCAGGTC TTTGATGCCC GGAGCTGTTC CACTGCCAAG
    GAAATGTTTG AGCACATCTG CAGGCACCTG CGTTATGCCA GCAACAATGG CAACATCAGG
    TCGGCCATCA CCGTGTTCCC CCAACGAACT GATGGGAAGC ATGACTTCCG AGTCTGGAAC
    GCCCAGCTCA TCCGCTATGC TGGCTACCAG ATGCCCGATG GCACCATCCT GGGGGACCCT
    GCCAGTGTGG AGTTCACCCA GCTGTGCATC GACCTGGGCT GGAAGCCCAA GTACGGCCGC
    TTTGATGTGG TGCCTCTGGT GCTGCAGGCT GATGGCCAAG ACCCTGAGTT CTTCGAAATC
    CCACCTGACC TTGTGCTCGA GGTACCCATG GAACATCCCA AGTATGAGTG GTTCCGGGAG
    CTGGAGCTGA AGTGGTACGC CCTGCCTGCA GTGGCCAACA TGCTGCTTGA GGTGGGTGGC
    CTTGAGTTCC CAGGGTGCCC CTTCAACGGC TGGTACATGG GCACGGAGAT TGGGGTCCGG
    GACTTCTGTG ATGTTCAGCG CTACAACATC CTGGAGGAAG TGGGCAGAAG GATGGGCCTG
    GAAACACACA AGCTGGCCTC ACTCTGGAAG GACCGGGCTG TCATTGAGAT CAATGTCGCT
    GTACTCCACA GTTTCCAGAA GCAAAATGTG ACCATCATGG ATCACCACTC AGCTGCAGAG
    TCCTTCATGA AGTACATGCA GAGCGAGTAC CGCTCCCGAG GGGGCTGCCC TGCCGACTGG
    ATTTGGCTGG TCCCCCCAAT TTCTGGGAGC ATCACTCCTG TGTTCCACCA GGAGATGTTA
    AATTATGTCC TGTCCCCTTT CTACTACTAC CAGGTGGAGG CCTGGAAAAC CCACGTGTGG
    CAGGATGAGA AGCGGAGACC CCAGAGGAGA AAGATTCAAC TTAAAGTCTT GGTTAAAGCT
    GTGCTTTTTG CCTCCATGCT GATGCGCAAG ACCATGGCAT CCCGAGTCAG AGTCACAATC
    CTCTTTGCGA CAGAGACGGG AAAGTCAGAA ACGCTAGCCC GGGACCTAGG GGCCTTGTTC
    AGCTGCGCCT TCCACCCCAA GGTTCTCTGC ATGGACGAGT ACAAGCTGAG CCACCTGGAG
    GAGGAGCAGC TACTGTTGGT GGTGACCAGC ACGTTTGGGA ACGGAGACTC CCCTGGCAAT
    GGAGAGAAAC TAAAAAAATC TCTCTTCATG CTGAAAGAGC TTACCAACAA GTTCAGGTAT
    GCCGTGTTTG GCCTCGGCTC CAGCATGTAC CCTCAGTTCT GTGCCTTTGC TCATGACATC
    GACCAGAAGC TATCGCATCT GGGGGCCTCT CAGCTCGCCC CAATAGGGGA AGGGGATGAG
    CTCAGTGGAC AGGAGGAAGC CTTCCGCTGC TGGGCCGTGC AAACCTTCAA GGCAGCCTGT
    GAGACGTTTG ATGTGCGAGG CAAACACTGC ATTCAGATCC CCAGGCTCTA CACTTCCAAT
    GTGACCTGGG ACCCACACCA ATACAGGCTC GTGCAGGACT CGCAGCCTCT GGACCTCAAC
    AAAGCCCTCA GCAGAATGCA TGCCAAGAAC GTGTTCACCC TGAGGCTCAA ATCACAACGG
    AATCTACAGA GTCCAAAATC CAGCCGCACC ACTCTCCTGG TCGAGCTCTC CTGTGAGGAC
    AGCCAGGAGC TGAGCTACCT GCCAGGAGAA CATCTCGGAG TTTTCCCAGG CAACCAGCTG
    GCCCTGGTGC AAGGCATCTT GGAGCGAGTA GTAGACAGCC CTGCACCCCA CCAGCCTGTG
    CACCTGGAGA CCCTCAGCGA GAGGGGAAGC TACTGGGTCA GAGACAAGCG GCTGCCTCCG
    TGCTCACTCA GCCAGGCCCT CACCTACTTC CTGGACATCA CCACTCCCCC GACCCAGCTG
    CTGCTCCGAA AGCTGGCACA GCTGGCCACA GAAGAGGCCG AGAGGCAGAG GTTGGAGATC
    CTGTGCCAGC CCTCAGAATA CAACAAGTGG AAGTTAACCA ACAGTCCCAC CTTCCTGGAG
    GTGCTGGAGG AGTTCCCATC CCTGCGGGTG TCTGCTGGCT TCCTGCTCTC CCAGCTCCCT
    ATTCTGAAAC CGCGGTACTA CTCCATCAGC TCCTCCAGGG ACTGCACACC CATGGAGGTC
    CACCTCACGG TGGCTGTGCT CGTGTACCCC ACCCGAGATG GCCAAGGTCC TTTGCACCAT
    GGTGTCTGTA GCACATGGCT CAGCAACCTG AAGCCCCAAG ACCCAGTACC CTGCTTTGTA
    CGAAGTGCCG GCAACTTCAA GCTCCCCGAG GATCCCTCCC GTCCTTGCAT CCTCATTGGA
    CCTGGCACAG GCATCGCCCC CTTCCGCAGT TTCTGGCAGC AGCGGCTCCA TGACATCAAG
    CACAAAGGGC TCCGAGGCAG CCGCATGACC CTGGTGTTTG GGTGCCGCCG CCCAGATGAG
    GACCACCTGT ATCGGGAGGA GATGTTGGAG ATGGCCCAGA GTGGGGTGCT GCATGAGGTG
    CACACAGCCT ATTCTCGCCT GCCTGGCCAG CCCAAGGTCT ATGTTCAAGA CATCCTGCGG
    CAGCAGCTGG CCAGCCAGGT GCTCCGCATG CTCCATGAGG AGCAGGGCCA CCTTTATGTC
    TGTGGGGATG TGCGTATGGC CCGGGATGTG GCCCATACCC TGAAGCACCT GGTGGCTGCC
    AAGCTGAGCC TGAGTGAAGA GCAAGTTGAG GACTATTTTT TCCAGCTTAA GAGCCAGAAG
    CGCTATCATG AAGATATCTT TGGTGCTGTG TTTCCCTATG AGGTGAAAAA AGATGGTGCA
    GCAAAACAGC CCAGCGATCC CAGAGTTCCA GCAGCCCACG GAAGAAGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe38270
    Clone ID Related Accession (Same CDS sequence) XM_014116464.1 , XM_014116464.2
    Accession Version XM_014116464.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nitric oxide synthase, inducible isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014116464.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCGCGAGG GGCTGGGCGC TGCGTGCGAG GCCGCCCGGC CCCGGCTCTG CCGCCTCCTC 
    CGCGGGATCC TGCGGCCGCA GCCCGCCTGC AGGCCCCCGC GAGCCAGTCT AGTGACACAG
    GATGACCCCA AGTGTCACAG CCTCAGCAAG CACCGGGATG AGTCCCCCCA GTCCCTCACA
    GGGACAGTAA AGACGTCTCC AGAATCCACA ATCAAGCCAG CTGCGCCCCC ACTGGCCTGC
    CCACGGCATG TGAGGATCAA AAACTGGGGC AACGGGATGA CCTTCCAAGA CACACTTCAC
    CACAAGGCCA AAGGGGATCT CGCTTGCAAG TCCAAGTCTT GTCTGGGAGC CATCATGAAC
    CCCAAAAGTT TGACCAGAGA ACCCAGGGAC AAGCCAACCC CCCCAGACGA GCTTCTACCT
    CAAGCCATTG AATTTGTCAA CCAGTATTAC AGCTCCTTCA AAGAGGCAAA AATAGAGGAA
    CATCTGGCCA GGGTGGAAGC AGTAACAAAG GAGATAGAAA CAACAGGAAC CTATCAGCTG
    ACGGGGGATG AGCTCATCTT CGCCACCAAG CAGGCCTGGC GCAACGCTCC CCGCTGCATC
    GGGAGGATCC AGTGGTCCAA CCTCCAGGTC TTTGATGCCC GGAGCTGTTC CACTGCCAAG
    GAAATGTTTG AGCACATCTG CAGGCACCTG CGTTATGCCA GCAACAATGG CAACATCAGG
    TCGGCCATCA CCGTGTTCCC CCAACGAACT GATGGGAAGC ATGACTTCCG AGTCTGGAAC
    GCCCAGCTCA TCCGCTATGC TGGCTACCAG ATGCCCGATG GCACCATCCT GGGGGACCCT
    GCCAGTGTGG AGTTCACCCA GCTGTGCATC GACCTGGGCT GGAAGCCCAA GTACGGCCGC
    TTTGATGTGG TGCCTCTGGT GCTGCAGGCT GATGGCCAAG ACCCTGAGTT CTTCGAAATC
    CCACCTGACC TTGTGCTCGA GGTACCCATG GAACATCCCA AGTATGAGTG GTTCCGGGAG
    CTGGAGCTGA AGTGGTACGC CCTGCCTGCA GTGGCCAACA TGCTGCTTGA GGTGGGTGGC
    CTTGAGTTCC CAGGGTGCCC CTTCAACGGC TGGTACATGG GCACGGAGAT TGGGGTCCGG
    GACTTCTGTG ATGTTCAGCG CTACAACATC CTGGAGGAAG TGGGCAGAAG GATGGGCCTG
    GAAACACACA AGCTGGCCTC ACTCTGGAAG GACCGGGCTG TCATTGAGAT CAATGTCGCT
    GTACTCCACA GTTTCCAGAA GCAAAATGTG ACCATCATGG ATCACCACTC AGCTGCAGAG
    TCCTTCATGA AGTACATGCA GAGCGAGTAC CGCTCCCGAG GGGGCTGCCC TGCCGACTGG
    ATTTGGCTGG TCCCCCCAAT TTCTGGGAGC ATCACTCCTG TGTTCCACCA GGAGATGTTA
    AATTATGTCC TGTCCCCTTT CTACTACTAC CAGGTGGAGG CCTGGAAAAC CCACGTGTGG
    CAGGATGAGA AGCGGAGACC CCAGAGGAGA AAGATTCAAC TTAAAGTCTT GGTTAAAGCT
    GTGCTTTTTG CCTCCATGCT GATGCGCAAG ACCATGGCAT CCCGAGTCAG AGTCACAATC
    CTCTTTGCGA CAGAGACGGG AAAGTCAGAA ACGCTAGCCC GGGACCTAGG GGCCTTGTTC
    AGCTGCGCCT TCCACCCCAA GGTTCTCTGC ATGGACGAGT ACAAGCTGAG CCACCTGGAG
    GAGGAGCAGC TACTGTTGGT GGTGACCAGC ACGTTTGGGA ACGGAGACTC CCCTGGCAAT
    GGAGAGAAAC TAAAAAAATC TCTCTTCATG CTGAAAGAGC TTACCAACAA GTTCAGGTAT
    GCCGTGTTTG GCCTCGGCTC CAGCATGTAC CCTCAGTTCT GTGCCTTTGC TCATGACATC
    GACCAGAAGC TATCGCATCT GGGGGCCTCT CAGCTCGCCC CAATAGGGGA AGGGGATGAG
    CTCAGTGGAC AGGAGGAAGC CTTCCGCTGC TGGGCCGTGC AAACCTTCAA GGCAGCCTGT
    GAGACGTTTG ATGTGCGAGG CAAACACTGC ATTCAGATCC CCAGGCTCTA CACTTCCAAT
    GTGACCTGGG ACCCACACCA ATACAGGCTC GTGCAGGACT CGCAGCCTCT GGACCTCAAC
    AAAGCCCTCA GCAGAATGCA TGCCAAGAAC GTGTTCACCC TGAGGCTCAA ATCACAACGG
    AATCTACAGA GTCCAAAATC CAGCCGCACC ACTCTCCTGG TCGAGCTCTC CTGTGAGGAC
    AGCCAGGAGC TGAGCTACCT GCCAGGAGAA CATCTCGGAG TTTTCCCAGG CAACCAGCTG
    GCCCTGGTGC AAGGCATCTT GGAGCGAGTA GTAGACAGCC CTGCACCCCA CCAGCCTGTG
    CACCTGGAGA CCCTCAGCGA GAGGGGAAGC TACTGGGTCA GAGACAAGCG GCTGCCTCCG
    TGCTCACTCA GCCAGGCCCT CACCTACTTC CTGGACATCA CCACTCCCCC GACCCAGCTG
    CTGCTCCGAA AGCTGGCACA GCTGGCCACA GAAGAGGCCG AGAGGCAGAG GTTGGAGATC
    CTGTGCCAGC CCTCAGAATA CAACAAGTGG AAGTTAACCA ACAGTCCCAC CTTCCTGGAG
    GTGCTGGAGG AGTTCCCATC CCTGCGGGTG TCTGCTGGCT TCCTGCTCTC CCAGCTCCCT
    ATTCTGAAAC CGCGGTACTA CTCCATCAGC TCCTCCAGGG ACTGCACACC CATGGAGGTC
    CACCTCACGG TGGCTGTGCT CGTGTACCCC ACCCGAGATG GCCAAGGTCC TTTGCACCAT
    GGTGTCTGTA GCACATGGCT CAGCAACCTG AAGCCCCAAG ACCCAGTACC CTGCTTTGTA
    CGAAGTGCCG GCAACTTCAA GCTCCCCGAG GATCCCTCCC GTCCTTGCAT CCTCATTGGA
    CCTGGCACAG GCATCGCCCC CTTCCGCAGT TTCTGGCAGC AGCGGCTCCA TGACATCAAG
    CACAAAGGGC TCCGAGGCAG CCGCATGACC CTGGTGTTTG GGTGCCGCCG CCCAGATGAG
    GACCACCTGT ATCGGGAGGA GATGTTGGAG ATGGCCCAGA GTGGGGTGCT GCATGAGGTG
    CACACAGCCT ATTCTCGCCT GCCTGGCCAG CCCAAGGTCT ATGTTCAAGA CATCCTGCGG
    CAGCAGCTGG CCAGCCAGGT GCTCCGCATG CTCCATGAGG AGCAGGGCCA CCTTTATGTC
    TGTGGGGATG TGCGTATGGC CCGGGATGTG GCCCATACCC TGAAGCACCT GGTGGCTGCC
    AAGCTGAGCC TGAGTGAAGA GCAAGTTGAG GACTATTTTT TCCAGCTTAA GAGCCAGAAG
    CGCTATCATG AAGATATCTT TGGTGCTGTG TTTCCCTATG AGGTGAAAAA AGATGGTGCA
    GCAAAACAGC CCAGCGATCC CAGAGTTCCA GCAGCCCACG GAAGAAGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013971939.1
    CDS37..3507
    Translation

    Target ORF information:

    RefSeq Version XM_014116464.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nitric oxide synthase 2 (NOS2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014116464.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCGCGAGG GGCTGGGCGC TGCGTGCGAG GCCGCCCGGC CCCGGCTCTG CCGCCTCCTC 
    CGCGGGATCC TGCGGCCGCA GCCCGCCTGC AGGCCCCCGC GAGCCAGTCT AGTGACACAG
    GATGACCCCA AGTGTCACAG CCTCAGCAAG CACCGGGATG AGTCCCCCCA GTCCCTCACA
    GGGACAGTAA AGACGTCTCC AGAATCCACA ATCAAGCCAG CTGCGCCCCC ACTGGCCTGC
    CCACGGCATG TGAGGATCAA AAACTGGGGC AACGGGATGA CCTTCCAAGA CACACTTCAC
    CACAAGGCCA AAGGGGATCT CGCTTGCAAG TCCAAGTCTT GTCTGGGAGC CATCATGAAC
    CCCAAAAGTT TGACCAGAGA ACCCAGGGAC AAGCCAACCC CCCCAGACGA GCTTCTACCT
    CAAGCCATTG AATTTGTCAA CCAGTATTAC AGCTCCTTCA AAGAGGCAAA AATAGAGGAA
    CATCTGGCCA GGGTGGAAGC AGTAACAAAG GAGATAGAAA CAACAGGAAC CTATCAGCTG
    ACGGGGGATG AGCTCATCTT CGCCACCAAG CAGGCCTGGC GCAACGCTCC CCGCTGCATC
    GGGAGGATCC AGTGGTCCAA CCTCCAGGTC TTTGATGCCC GGAGCTGTTC CACTGCCAAG
    GAAATGTTTG AGCACATCTG CAGGCACCTG CGTTATGCCA GCAACAATGG CAACATCAGG
    TCGGCCATCA CCGTGTTCCC CCAACGAACT GATGGGAAGC ATGACTTCCG AGTCTGGAAC
    GCCCAGCTCA TCCGCTATGC TGGCTACCAG ATGCCCGATG GCACCATCCT GGGGGACCCT
    GCCAGTGTGG AGTTCACCCA GCTGTGCATC GACCTGGGCT GGAAGCCCAA GTACGGCCGC
    TTTGATGTGG TGCCTCTGGT GCTGCAGGCT GATGGCCAAG ACCCTGAGTT CTTCGAAATC
    CCACCTGACC TTGTGCTCGA GGTACCCATG GAACATCCCA AGTATGAGTG GTTCCGGGAG
    CTGGAGCTGA AGTGGTACGC CCTGCCTGCA GTGGCCAACA TGCTGCTTGA GGTGGGTGGC
    CTTGAGTTCC CAGGGTGCCC CTTCAACGGC TGGTACATGG GCACGGAGAT TGGGGTCCGG
    GACTTCTGTG ATGTTCAGCG CTACAACATC CTGGAGGAAG TGGGCAGAAG GATGGGCCTG
    GAAACACACA AGCTGGCCTC ACTCTGGAAG GACCGGGCTG TCATTGAGAT CAATGTCGCT
    GTACTCCACA GTTTCCAGAA GCAAAATGTG ACCATCATGG ATCACCACTC AGCTGCAGAG
    TCCTTCATGA AGTACATGCA GAGCGAGTAC CGCTCCCGAG GGGGCTGCCC TGCCGACTGG
    ATTTGGCTGG TCCCCCCAAT TTCTGGGAGC ATCACTCCTG TGTTCCACCA GGAGATGTTA
    AATTATGTCC TGTCCCCTTT CTACTACTAC CAGGTGGAGG CCTGGAAAAC CCACGTGTGG
    CAGGATGAGA AGCGGAGACC CCAGAGGAGA AAGATTCAAC TTAAAGTCTT GGTTAAAGCT
    GTGCTTTTTG CCTCCATGCT GATGCGCAAG ACCATGGCAT CCCGAGTCAG AGTCACAATC
    CTCTTTGCGA CAGAGACGGG AAAGTCAGAA ACGCTAGCCC GGGACCTAGG GGCCTTGTTC
    AGCTGCGCCT TCCACCCCAA GGTTCTCTGC ATGGACGAGT ACAAGCTGAG CCACCTGGAG
    GAGGAGCAGC TACTGTTGGT GGTGACCAGC ACGTTTGGGA ACGGAGACTC CCCTGGCAAT
    GGAGAGAAAC TAAAAAAATC TCTCTTCATG CTGAAAGAGC TTACCAACAA GTTCAGGTAT
    GCCGTGTTTG GCCTCGGCTC CAGCATGTAC CCTCAGTTCT GTGCCTTTGC TCATGACATC
    GACCAGAAGC TATCGCATCT GGGGGCCTCT CAGCTCGCCC CAATAGGGGA AGGGGATGAG
    CTCAGTGGAC AGGAGGAAGC CTTCCGCTGC TGGGCCGTGC AAACCTTCAA GGCAGCCTGT
    GAGACGTTTG ATGTGCGAGG CAAACACTGC ATTCAGATCC CCAGGCTCTA CACTTCCAAT
    GTGACCTGGG ACCCACACCA ATACAGGCTC GTGCAGGACT CGCAGCCTCT GGACCTCAAC
    AAAGCCCTCA GCAGAATGCA TGCCAAGAAC GTGTTCACCC TGAGGCTCAA ATCACAACGG
    AATCTACAGA GTCCAAAATC CAGCCGCACC ACTCTCCTGG TCGAGCTCTC CTGTGAGGAC
    AGCCAGGAGC TGAGCTACCT GCCAGGAGAA CATCTCGGAG TTTTCCCAGG CAACCAGCTG
    GCCCTGGTGC AAGGCATCTT GGAGCGAGTA GTAGACAGCC CTGCACCCCA CCAGCCTGTG
    CACCTGGAGA CCCTCAGCGA GAGGGGAAGC TACTGGGTCA GAGACAAGCG GCTGCCTCCG
    TGCTCACTCA GCCAGGCCCT CACCTACTTC CTGGACATCA CCACTCCCCC GACCCAGCTG
    CTGCTCCGAA AGCTGGCACA GCTGGCCACA GAAGAGGCCG AGAGGCAGAG GTTGGAGATC
    CTGTGCCAGC CCTCAGAATA CAACAAGTGG AAGTTAACCA ACAGTCCCAC CTTCCTGGAG
    GTGCTGGAGG AGTTCCCATC CCTGCGGGTG TCTGCTGGCT TCCTGCTCTC CCAGCTCCCT
    ATTCTGAAAC CGCGGTACTA CTCCATCAGC TCCTCCAGGG ACTGCACACC CATGGAGGTC
    CACCTCACGG TGGCTGTGCT CGTGTACCCC ACCCGAGATG GCCAAGGTCC TTTGCACCAT
    GGTGTCTGTA GCACATGGCT CAGCAACCTG AAGCCCCAAG ACCCAGTACC CTGCTTTGTA
    CGAAGTGCCG GCAACTTCAA GCTCCCCGAG GATCCCTCCC GTCCTTGCAT CCTCATTGGA
    CCTGGCACAG GCATCGCCCC CTTCCGCAGT TTCTGGCAGC AGCGGCTCCA TGACATCAAG
    CACAAAGGGC TCCGAGGCAG CCGCATGACC CTGGTGTTTG GGTGCCGCCG CCCAGATGAG
    GACCACCTGT ATCGGGAGGA GATGTTGGAG ATGGCCCAGA GTGGGGTGCT GCATGAGGTG
    CACACAGCCT ATTCTCGCCT GCCTGGCCAG CCCAAGGTCT ATGTTCAAGA CATCCTGCGG
    CAGCAGCTGG CCAGCCAGGT GCTCCGCATG CTCCATGAGG AGCAGGGCCA CCTTTATGTC
    TGTGGGGATG TGCGTATGGC CCGGGATGTG GCCCATACCC TGAAGCACCT GGTGGCTGCC
    AAGCTGAGCC TGAGTGAAGA GCAAGTTGAG GACTATTTTT TCCAGCTTAA GAGCCAGAAG
    CGCTATCATG AAGATATCTT TGGTGCTGTG TTTCCCTATG AGGTGAAAAA AGATGGTGCA
    GCAAAACAGC CCAGCGATCC CAGAGTTCCA GCAGCCCACG GAAGAAGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe38271
    Clone ID Related Accession (Same CDS sequence) XM_014116465.2 , XM_014116465.1
    Accession Version XM_014116465.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3195bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nitric oxide synthase, inducible isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014116465.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCTTCC AAGACACACT TCACCACAAG GCCAAAGGGG ATCTCGCTTG CAAGTCCAAG 
    TCTTGTCTGG GAGCCATCAT GAACCCCAAA AGTTTGACCA GAGAACCCAG GGACAAGCCA
    ACCCCCCCAG ACGAGCTTCT ACCTCAAGCC ATTGAATTTG TCAACCAGTA TTACAGCTCC
    TTCAAAGAGG CAAAAATAGA GGAACATCTG GCCAGGGTGG AAGCAGTAAC AAAGGAGATA
    GAAACAACAG GAACCTATCA GCTGACGGGG GATGAGCTCA TCTTCGCCAC CAAGCAGGCC
    TGGCGCAACG CTCCCCGCTG CATCGGGAGG ATCCAGTGGT CCAACCTCCA GGTCTTTGAT
    GCCCGGAGCT GTTCCACTGC CAAGGAAATG TTTGAGCACA TCTGCAGGCA CCTGCGTTAT
    GCCAGCAACA ATGGCAACAT CAGGTCGGCC ATCACCGTGT TCCCCCAACG AACTGATGGG
    AAGCATGACT TCCGAGTCTG GAACGCCCAG CTCATCCGCT ATGCTGGCTA CCAGATGCCC
    GATGGCACCA TCCTGGGGGA CCCTGCCAGT GTGGAGTTCA CCCAGCTGTG CATCGACCTG
    GGCTGGAAGC CCAAGTACGG CCGCTTTGAT GTGGTGCCTC TGGTGCTGCA GGCTGATGGC
    CAAGACCCTG AGTTCTTCGA AATCCCACCT GACCTTGTGC TCGAGGTACC CATGGAACAT
    CCCAAGTATG AGTGGTTCCG GGAGCTGGAG CTGAAGTGGT ACGCCCTGCC TGCAGTGGCC
    AACATGCTGC TTGAGGTGGG TGGCCTTGAG TTCCCAGGGT GCCCCTTCAA CGGCTGGTAC
    ATGGGCACGG AGATTGGGGT CCGGGACTTC TGTGATGTTC AGCGCTACAA CATCCTGGAG
    GAAGTGGGCA GAAGGATGGG CCTGGAAACA CACAAGCTGG CCTCACTCTG GAAGGACCGG
    GCTGTCATTG AGATCAATGT CGCTGTACTC CACAGTTTCC AGAAGCAAAA TGTGACCATC
    ATGGATCACC ACTCAGCTGC AGAGTCCTTC ATGAAGTACA TGCAGAGCGA GTACCGCTCC
    CGAGGGGGCT GCCCTGCCGA CTGGATTTGG CTGGTCCCCC CAATTTCTGG GAGCATCACT
    CCTGTGTTCC ACCAGGAGAT GTTAAATTAT GTCCTGTCCC CTTTCTACTA CTACCAGGTG
    GAGGCCTGGA AAACCCACGT GTGGCAGGAT GAGAAGCGGA GACCCCAGAG GAGAAAGATT
    CAACTTAAAG TCTTGGTTAA AGCTGTGCTT TTTGCCTCCA TGCTGATGCG CAAGACCATG
    GCATCCCGAG TCAGAGTCAC AATCCTCTTT GCGACAGAGA CGGGAAAGTC AGAAACGCTA
    GCCCGGGACC TAGGGGCCTT GTTCAGCTGC GCCTTCCACC CCAAGGTTCT CTGCATGGAC
    GAGTACAAGC TGAGCCACCT GGAGGAGGAG CAGCTACTGT TGGTGGTGAC CAGCACGTTT
    GGGAACGGAG ACTCCCCTGG CAATGGAGAG AAACTAAAAA AATCTCTCTT CATGCTGAAA
    GAGCTTACCA ACAAGTTCAG GTATGCCGTG TTTGGCCTCG GCTCCAGCAT GTACCCTCAG
    TTCTGTGCCT TTGCTCATGA CATCGACCAG AAGCTATCGC ATCTGGGGGC CTCTCAGCTC
    GCCCCAATAG GGGAAGGGGA TGAGCTCAGT GGACAGGAGG AAGCCTTCCG CTGCTGGGCC
    GTGCAAACCT TCAAGGCAGC CTGTGAGACG TTTGATGTGC GAGGCAAACA CTGCATTCAG
    ATCCCCAGGC TCTACACTTC CAATGTGACC TGGGACCCAC ACCAATACAG GCTCGTGCAG
    GACTCGCAGC CTCTGGACCT CAACAAAGCC CTCAGCAGAA TGCATGCCAA GAACGTGTTC
    ACCCTGAGGC TCAAATCACA ACGGAATCTA CAGAGTCCAA AATCCAGCCG CACCACTCTC
    CTGGTCGAGC TCTCCTGTGA GGACAGCCAG GAGCTGAGCT ACCTGCCAGG AGAACATCTC
    GGAGTTTTCC CAGGCAACCA GCTGGCCCTG GTGCAAGGCA TCTTGGAGCG AGTAGTAGAC
    AGCCCTGCAC CCCACCAGCC TGTGCACCTG GAGACCCTCA GCGAGAGGGG AAGCTACTGG
    GTCAGAGACA AGCGGCTGCC TCCGTGCTCA CTCAGCCAGG CCCTCACCTA CTTCCTGGAC
    ATCACCACTC CCCCGACCCA GCTGCTGCTC CGAAAGCTGG CACAGCTGGC CACAGAAGAG
    GCCGAGAGGC AGAGGTTGGA GATCCTGTGC CAGCCCTCAG AATACAACAA GTGGAAGTTA
    ACCAACAGTC CCACCTTCCT GGAGGTGCTG GAGGAGTTCC CATCCCTGCG GGTGTCTGCT
    GGCTTCCTGC TCTCCCAGCT CCCTATTCTG AAACCGCGGT ACTACTCCAT CAGCTCCTCC
    AGGGACTGCA CACCCATGGA GGTCCACCTC ACGGTGGCTG TGCTCGTGTA CCCCACCCGA
    GATGGCCAAG GTCCTTTGCA CCATGGTGTC TGTAGCACAT GGCTCAGCAA CCTGAAGCCC
    CAAGACCCAG TACCCTGCTT TGTACGAAGT GCCGGCAACT TCAAGCTCCC CGAGGATCCC
    TCCCGTCCTT GCATCCTCAT TGGACCTGGC ACAGGCATCG CCCCCTTCCG CAGTTTCTGG
    CAGCAGCGGC TCCATGACAT CAAGCACAAA GGGCTCCGAG GCAGCCGCAT GACCCTGGTG
    TTTGGGTGCC GCCGCCCAGA TGAGGACCAC CTGTATCGGG AGGAGATGTT GGAGATGGCC
    CAGAGTGGGG TGCTGCATGA GGTGCACACA GCCTATTCTC GCCTGCCTGG CCAGCCCAAG
    GTCTATGTTC AAGACATCCT GCGGCAGCAG CTGGCCAGCC AGGTGCTCCG CATGCTCCAT
    GAGGAGCAGG GCCACCTTTA TGTCTGTGGG GATGTGCGTA TGGCCCGGGA TGTGGCCCAT
    ACCCTGAAGC ACCTGGTGGC TGCCAAGCTG AGCCTGAGTG AAGAGCAAGT TGAGGACTAT
    TTTTTCCAGC TTAAGAGCCA GAAGCGCTAT CATGAAGATA TCTTTGGTGC TGTGTTTCCC
    TATGAGGTGA AAAAAGATGG TGCAGCAAAA CAGCCCAGCG ATCCCAGAGT TCCAGCAGCC
    CACGGAAGAA GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013971940.1
    CDS281..3475
    Translation

    Target ORF information:

    RefSeq Version XM_014116465.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nitric oxide synthase 2 (NOS2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014116465.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCTTCC AAGACACACT TCACCACAAG GCCAAAGGGG ATCTCGCTTG CAAGTCCAAG 
    TCTTGTCTGG GAGCCATCAT GAACCCCAAA AGTTTGACCA GAGAACCCAG GGACAAGCCA
    ACCCCCCCAG ACGAGCTTCT ACCTCAAGCC ATTGAATTTG TCAACCAGTA TTACAGCTCC
    TTCAAAGAGG CAAAAATAGA GGAACATCTG GCCAGGGTGG AAGCAGTAAC AAAGGAGATA
    GAAACAACAG GAACCTATCA GCTGACGGGG GATGAGCTCA TCTTCGCCAC CAAGCAGGCC
    TGGCGCAACG CTCCCCGCTG CATCGGGAGG ATCCAGTGGT CCAACCTCCA GGTCTTTGAT
    GCCCGGAGCT GTTCCACTGC CAAGGAAATG TTTGAGCACA TCTGCAGGCA CCTGCGTTAT
    GCCAGCAACA ATGGCAACAT CAGGTCGGCC ATCACCGTGT TCCCCCAACG AACTGATGGG
    AAGCATGACT TCCGAGTCTG GAACGCCCAG CTCATCCGCT ATGCTGGCTA CCAGATGCCC
    GATGGCACCA TCCTGGGGGA CCCTGCCAGT GTGGAGTTCA CCCAGCTGTG CATCGACCTG
    GGCTGGAAGC CCAAGTACGG CCGCTTTGAT GTGGTGCCTC TGGTGCTGCA GGCTGATGGC
    CAAGACCCTG AGTTCTTCGA AATCCCACCT GACCTTGTGC TCGAGGTACC CATGGAACAT
    CCCAAGTATG AGTGGTTCCG GGAGCTGGAG CTGAAGTGGT ACGCCCTGCC TGCAGTGGCC
    AACATGCTGC TTGAGGTGGG TGGCCTTGAG TTCCCAGGGT GCCCCTTCAA CGGCTGGTAC
    ATGGGCACGG AGATTGGGGT CCGGGACTTC TGTGATGTTC AGCGCTACAA CATCCTGGAG
    GAAGTGGGCA GAAGGATGGG CCTGGAAACA CACAAGCTGG CCTCACTCTG GAAGGACCGG
    GCTGTCATTG AGATCAATGT CGCTGTACTC CACAGTTTCC AGAAGCAAAA TGTGACCATC
    ATGGATCACC ACTCAGCTGC AGAGTCCTTC ATGAAGTACA TGCAGAGCGA GTACCGCTCC
    CGAGGGGGCT GCCCTGCCGA CTGGATTTGG CTGGTCCCCC CAATTTCTGG GAGCATCACT
    CCTGTGTTCC ACCAGGAGAT GTTAAATTAT GTCCTGTCCC CTTTCTACTA CTACCAGGTG
    GAGGCCTGGA AAACCCACGT GTGGCAGGAT GAGAAGCGGA GACCCCAGAG GAGAAAGATT
    CAACTTAAAG TCTTGGTTAA AGCTGTGCTT TTTGCCTCCA TGCTGATGCG CAAGACCATG
    GCATCCCGAG TCAGAGTCAC AATCCTCTTT GCGACAGAGA CGGGAAAGTC AGAAACGCTA
    GCCCGGGACC TAGGGGCCTT GTTCAGCTGC GCCTTCCACC CCAAGGTTCT CTGCATGGAC
    GAGTACAAGC TGAGCCACCT GGAGGAGGAG CAGCTACTGT TGGTGGTGAC CAGCACGTTT
    GGGAACGGAG ACTCCCCTGG CAATGGAGAG AAACTAAAAA AATCTCTCTT CATGCTGAAA
    GAGCTTACCA ACAAGTTCAG GTATGCCGTG TTTGGCCTCG GCTCCAGCAT GTACCCTCAG
    TTCTGTGCCT TTGCTCATGA CATCGACCAG AAGCTATCGC ATCTGGGGGC CTCTCAGCTC
    GCCCCAATAG GGGAAGGGGA TGAGCTCAGT GGACAGGAGG AAGCCTTCCG CTGCTGGGCC
    GTGCAAACCT TCAAGGCAGC CTGTGAGACG TTTGATGTGC GAGGCAAACA CTGCATTCAG
    ATCCCCAGGC TCTACACTTC CAATGTGACC TGGGACCCAC ACCAATACAG GCTCGTGCAG
    GACTCGCAGC CTCTGGACCT CAACAAAGCC CTCAGCAGAA TGCATGCCAA GAACGTGTTC
    ACCCTGAGGC TCAAATCACA ACGGAATCTA CAGAGTCCAA AATCCAGCCG CACCACTCTC
    CTGGTCGAGC TCTCCTGTGA GGACAGCCAG GAGCTGAGCT ACCTGCCAGG AGAACATCTC
    GGAGTTTTCC CAGGCAACCA GCTGGCCCTG GTGCAAGGCA TCTTGGAGCG AGTAGTAGAC
    AGCCCTGCAC CCCACCAGCC TGTGCACCTG GAGACCCTCA GCGAGAGGGG AAGCTACTGG
    GTCAGAGACA AGCGGCTGCC TCCGTGCTCA CTCAGCCAGG CCCTCACCTA CTTCCTGGAC
    ATCACCACTC CCCCGACCCA GCTGCTGCTC CGAAAGCTGG CACAGCTGGC CACAGAAGAG
    GCCGAGAGGC AGAGGTTGGA GATCCTGTGC CAGCCCTCAG AATACAACAA GTGGAAGTTA
    ACCAACAGTC CCACCTTCCT GGAGGTGCTG GAGGAGTTCC CATCCCTGCG GGTGTCTGCT
    GGCTTCCTGC TCTCCCAGCT CCCTATTCTG AAACCGCGGT ACTACTCCAT CAGCTCCTCC
    AGGGACTGCA CACCCATGGA GGTCCACCTC ACGGTGGCTG TGCTCGTGTA CCCCACCCGA
    GATGGCCAAG GTCCTTTGCA CCATGGTGTC TGTAGCACAT GGCTCAGCAA CCTGAAGCCC
    CAAGACCCAG TACCCTGCTT TGTACGAAGT GCCGGCAACT TCAAGCTCCC CGAGGATCCC
    TCCCGTCCTT GCATCCTCAT TGGACCTGGC ACAGGCATCG CCCCCTTCCG CAGTTTCTGG
    CAGCAGCGGC TCCATGACAT CAAGCACAAA GGGCTCCGAG GCAGCCGCAT GACCCTGGTG
    TTTGGGTGCC GCCGCCCAGA TGAGGACCAC CTGTATCGGG AGGAGATGTT GGAGATGGCC
    CAGAGTGGGG TGCTGCATGA GGTGCACACA GCCTATTCTC GCCTGCCTGG CCAGCCCAAG
    GTCTATGTTC AAGACATCCT GCGGCAGCAG CTGGCCAGCC AGGTGCTCCG CATGCTCCAT
    GAGGAGCAGG GCCACCTTTA TGTCTGTGGG GATGTGCGTA TGGCCCGGGA TGTGGCCCAT
    ACCCTGAAGC ACCTGGTGGC TGCCAAGCTG AGCCTGAGTG AAGAGCAAGT TGAGGACTAT
    TTTTTCCAGC TTAAGAGCCA GAAGCGCTAT CATGAAGATA TCTTTGGTGC TGTGTTTCCC
    TATGAGGTGA AAAAAGATGG TGCAGCAAAA CAGCCCAGCG ATCCCAGAGT TCCAGCAGCC
    CACGGAAGAA GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe38271
    Clone ID Related Accession (Same CDS sequence) XM_014116465.2 , XM_014116465.1
    Accession Version XM_014116465.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3195bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nitric oxide synthase, inducible isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCTTCC AAGACACACT TCACCACAAG GCCAAAGGGG ATCTCGCTTG CAAGTCCAAG 
    TCTTGTCTGG GAGCCATCAT GAACCCCAAA AGTTTGACCA GAGAACCCAG GGACAAGCCA
    ACCCCCCCAG ACGAGCTTCT ACCTCAAGCC ATTGAATTTG TCAACCAGTA TTACAGCTCC
    TTCAAAGAGG CAAAAATAGA GGAACATCTG GCCAGGGTGG AAGCAGTAAC AAAGGAGATA
    GAAACAACAG GAACCTATCA GCTGACGGGG GATGAGCTCA TCTTCGCCAC CAAGCAGGCC
    TGGCGCAACG CTCCCCGCTG CATCGGGAGG ATCCAGTGGT CCAACCTCCA GGTCTTTGAT
    GCCCGGAGCT GTTCCACTGC CAAGGAAATG TTTGAGCACA TCTGCAGGCA CCTGCGTTAT
    GCCAGCAACA ATGGCAACAT CAGGTCGGCC ATCACCGTGT TCCCCCAACG AACTGATGGG
    AAGCATGACT TCCGAGTCTG GAACGCCCAG CTCATCCGCT ATGCTGGCTA CCAGATGCCC
    GATGGCACCA TCCTGGGGGA CCCTGCCAGT GTGGAGTTCA CCCAGCTGTG CATCGACCTG
    GGCTGGAAGC CCAAGTACGG CCGCTTTGAT GTGGTGCCTC TGGTGCTGCA GGCTGATGGC
    CAAGACCCTG AGTTCTTCGA AATCCCACCT GACCTTGTGC TCGAGGTACC CATGGAACAT
    CCCAAGTATG AGTGGTTCCG GGAGCTGGAG CTGAAGTGGT ACGCCCTGCC TGCAGTGGCC
    AACATGCTGC TTGAGGTGGG TGGCCTTGAG TTCCCAGGGT GCCCCTTCAA CGGCTGGTAC
    ATGGGCACGG AGATTGGGGT CCGGGACTTC TGTGATGTTC AGCGCTACAA CATCCTGGAG
    GAAGTGGGCA GAAGGATGGG CCTGGAAACA CACAAGCTGG CCTCACTCTG GAAGGACCGG
    GCTGTCATTG AGATCAATGT CGCTGTACTC CACAGTTTCC AGAAGCAAAA TGTGACCATC
    ATGGATCACC ACTCAGCTGC AGAGTCCTTC ATGAAGTACA TGCAGAGCGA GTACCGCTCC
    CGAGGGGGCT GCCCTGCCGA CTGGATTTGG CTGGTCCCCC CAATTTCTGG GAGCATCACT
    CCTGTGTTCC ACCAGGAGAT GTTAAATTAT GTCCTGTCCC CTTTCTACTA CTACCAGGTG
    GAGGCCTGGA AAACCCACGT GTGGCAGGAT GAGAAGCGGA GACCCCAGAG GAGAAAGATT
    CAACTTAAAG TCTTGGTTAA AGCTGTGCTT TTTGCCTCCA TGCTGATGCG CAAGACCATG
    GCATCCCGAG TCAGAGTCAC AATCCTCTTT GCGACAGAGA CGGGAAAGTC AGAAACGCTA
    GCCCGGGACC TAGGGGCCTT GTTCAGCTGC GCCTTCCACC CCAAGGTTCT CTGCATGGAC
    GAGTACAAGC TGAGCCACCT GGAGGAGGAG CAGCTACTGT TGGTGGTGAC CAGCACGTTT
    GGGAACGGAG ACTCCCCTGG CAATGGAGAG AAACTAAAAA AATCTCTCTT CATGCTGAAA
    GAGCTTACCA ACAAGTTCAG GTATGCCGTG TTTGGCCTCG GCTCCAGCAT GTACCCTCAG
    TTCTGTGCCT TTGCTCATGA CATCGACCAG AAGCTATCGC ATCTGGGGGC CTCTCAGCTC
    GCCCCAATAG GGGAAGGGGA TGAGCTCAGT GGACAGGAGG AAGCCTTCCG CTGCTGGGCC
    GTGCAAACCT TCAAGGCAGC CTGTGAGACG TTTGATGTGC GAGGCAAACA CTGCATTCAG
    ATCCCCAGGC TCTACACTTC CAATGTGACC TGGGACCCAC ACCAATACAG GCTCGTGCAG
    GACTCGCAGC CTCTGGACCT CAACAAAGCC CTCAGCAGAA TGCATGCCAA GAACGTGTTC
    ACCCTGAGGC TCAAATCACA ACGGAATCTA CAGAGTCCAA AATCCAGCCG CACCACTCTC
    CTGGTCGAGC TCTCCTGTGA GGACAGCCAG GAGCTGAGCT ACCTGCCAGG AGAACATCTC
    GGAGTTTTCC CAGGCAACCA GCTGGCCCTG GTGCAAGGCA TCTTGGAGCG AGTAGTAGAC
    AGCCCTGCAC CCCACCAGCC TGTGCACCTG GAGACCCTCA GCGAGAGGGG AAGCTACTGG
    GTCAGAGACA AGCGGCTGCC TCCGTGCTCA CTCAGCCAGG CCCTCACCTA CTTCCTGGAC
    ATCACCACTC CCCCGACCCA GCTGCTGCTC CGAAAGCTGG CACAGCTGGC CACAGAAGAG
    GCCGAGAGGC AGAGGTTGGA GATCCTGTGC CAGCCCTCAG AATACAACAA GTGGAAGTTA
    ACCAACAGTC CCACCTTCCT GGAGGTGCTG GAGGAGTTCC CATCCCTGCG GGTGTCTGCT
    GGCTTCCTGC TCTCCCAGCT CCCTATTCTG AAACCGCGGT ACTACTCCAT CAGCTCCTCC
    AGGGACTGCA CACCCATGGA GGTCCACCTC ACGGTGGCTG TGCTCGTGTA CCCCACCCGA
    GATGGCCAAG GTCCTTTGCA CCATGGTGTC TGTAGCACAT GGCTCAGCAA CCTGAAGCCC
    CAAGACCCAG TACCCTGCTT TGTACGAAGT GCCGGCAACT TCAAGCTCCC CGAGGATCCC
    TCCCGTCCTT GCATCCTCAT TGGACCTGGC ACAGGCATCG CCCCCTTCCG CAGTTTCTGG
    CAGCAGCGGC TCCATGACAT CAAGCACAAA GGGCTCCGAG GCAGCCGCAT GACCCTGGTG
    TTTGGGTGCC GCCGCCCAGA TGAGGACCAC CTGTATCGGG AGGAGATGTT GGAGATGGCC
    CAGAGTGGGG TGCTGCATGA GGTGCACACA GCCTATTCTC GCCTGCCTGG CCAGCCCAAG
    GTCTATGTTC AAGACATCCT GCGGCAGCAG CTGGCCAGCC AGGTGCTCCG CATGCTCCAT
    GAGGAGCAGG GCCACCTTTA TGTCTGTGGG GATGTGCGTA TGGCCCGGGA TGTGGCCCAT
    ACCCTGAAGC ACCTGGTGGC TGCCAAGCTG AGCCTGAGTG AAGAGCAAGT TGAGGACTAT
    TTTTTCCAGC TTAAGAGCCA GAAGCGCTAT CATGAAGATA TCTTTGGTGC TGTGTTTCCC
    TATGAGGTGA AAAAAGATGG TGCAGCAAAA CAGCCCAGCG ATCCCAGAGT TCCAGCAGCC
    CACGGAAGAA GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013971940.1
    CDS311..3505
    Translation

    Target ORF information:

    RefSeq Version XM_014116465.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nitric oxide synthase 2, inducible (NOS2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014116465.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCTTCC AAGACACACT TCACCACAAG GCCAAAGGGG ATCTCGCTTG CAAGTCCAAG 
    TCTTGTCTGG GAGCCATCAT GAACCCCAAA AGTTTGACCA GAGAACCCAG GGACAAGCCA
    ACCCCCCCAG ACGAGCTTCT ACCTCAAGCC ATTGAATTTG TCAACCAGTA TTACAGCTCC
    TTCAAAGAGG CAAAAATAGA GGAACATCTG GCCAGGGTGG AAGCAGTAAC AAAGGAGATA
    GAAACAACAG GAACCTATCA GCTGACGGGG GATGAGCTCA TCTTCGCCAC CAAGCAGGCC
    TGGCGCAACG CTCCCCGCTG CATCGGGAGG ATCCAGTGGT CCAACCTCCA GGTCTTTGAT
    GCCCGGAGCT GTTCCACTGC CAAGGAAATG TTTGAGCACA TCTGCAGGCA CCTGCGTTAT
    GCCAGCAACA ATGGCAACAT CAGGTCGGCC ATCACCGTGT TCCCCCAACG AACTGATGGG
    AAGCATGACT TCCGAGTCTG GAACGCCCAG CTCATCCGCT ATGCTGGCTA CCAGATGCCC
    GATGGCACCA TCCTGGGGGA CCCTGCCAGT GTGGAGTTCA CCCAGCTGTG CATCGACCTG
    GGCTGGAAGC CCAAGTACGG CCGCTTTGAT GTGGTGCCTC TGGTGCTGCA GGCTGATGGC
    CAAGACCCTG AGTTCTTCGA AATCCCACCT GACCTTGTGC TCGAGGTACC CATGGAACAT
    CCCAAGTATG AGTGGTTCCG GGAGCTGGAG CTGAAGTGGT ACGCCCTGCC TGCAGTGGCC
    AACATGCTGC TTGAGGTGGG TGGCCTTGAG TTCCCAGGGT GCCCCTTCAA CGGCTGGTAC
    ATGGGCACGG AGATTGGGGT CCGGGACTTC TGTGATGTTC AGCGCTACAA CATCCTGGAG
    GAAGTGGGCA GAAGGATGGG CCTGGAAACA CACAAGCTGG CCTCACTCTG GAAGGACCGG
    GCTGTCATTG AGATCAATGT CGCTGTACTC CACAGTTTCC AGAAGCAAAA TGTGACCATC
    ATGGATCACC ACTCAGCTGC AGAGTCCTTC ATGAAGTACA TGCAGAGCGA GTACCGCTCC
    CGAGGGGGCT GCCCTGCCGA CTGGATTTGG CTGGTCCCCC CAATTTCTGG GAGCATCACT
    CCTGTGTTCC ACCAGGAGAT GTTAAATTAT GTCCTGTCCC CTTTCTACTA CTACCAGGTG
    GAGGCCTGGA AAACCCACGT GTGGCAGGAT GAGAAGCGGA GACCCCAGAG GAGAAAGATT
    CAACTTAAAG TCTTGGTTAA AGCTGTGCTT TTTGCCTCCA TGCTGATGCG CAAGACCATG
    GCATCCCGAG TCAGAGTCAC AATCCTCTTT GCGACAGAGA CGGGAAAGTC AGAAACGCTA
    GCCCGGGACC TAGGGGCCTT GTTCAGCTGC GCCTTCCACC CCAAGGTTCT CTGCATGGAC
    GAGTACAAGC TGAGCCACCT GGAGGAGGAG CAGCTACTGT TGGTGGTGAC CAGCACGTTT
    GGGAACGGAG ACTCCCCTGG CAATGGAGAG AAACTAAAAA AATCTCTCTT CATGCTGAAA
    GAGCTTACCA ACAAGTTCAG GTATGCCGTG TTTGGCCTCG GCTCCAGCAT GTACCCTCAG
    TTCTGTGCCT TTGCTCATGA CATCGACCAG AAGCTATCGC ATCTGGGGGC CTCTCAGCTC
    GCCCCAATAG GGGAAGGGGA TGAGCTCAGT GGACAGGAGG AAGCCTTCCG CTGCTGGGCC
    GTGCAAACCT TCAAGGCAGC CTGTGAGACG TTTGATGTGC GAGGCAAACA CTGCATTCAG
    ATCCCCAGGC TCTACACTTC CAATGTGACC TGGGACCCAC ACCAATACAG GCTCGTGCAG
    GACTCGCAGC CTCTGGACCT CAACAAAGCC CTCAGCAGAA TGCATGCCAA GAACGTGTTC
    ACCCTGAGGC TCAAATCACA ACGGAATCTA CAGAGTCCAA AATCCAGCCG CACCACTCTC
    CTGGTCGAGC TCTCCTGTGA GGACAGCCAG GAGCTGAGCT ACCTGCCAGG AGAACATCTC
    GGAGTTTTCC CAGGCAACCA GCTGGCCCTG GTGCAAGGCA TCTTGGAGCG AGTAGTAGAC
    AGCCCTGCAC CCCACCAGCC TGTGCACCTG GAGACCCTCA GCGAGAGGGG AAGCTACTGG
    GTCAGAGACA AGCGGCTGCC TCCGTGCTCA CTCAGCCAGG CCCTCACCTA CTTCCTGGAC
    ATCACCACTC CCCCGACCCA GCTGCTGCTC CGAAAGCTGG CACAGCTGGC CACAGAAGAG
    GCCGAGAGGC AGAGGTTGGA GATCCTGTGC CAGCCCTCAG AATACAACAA GTGGAAGTTA
    ACCAACAGTC CCACCTTCCT GGAGGTGCTG GAGGAGTTCC CATCCCTGCG GGTGTCTGCT
    GGCTTCCTGC TCTCCCAGCT CCCTATTCTG AAACCGCGGT ACTACTCCAT CAGCTCCTCC
    AGGGACTGCA CACCCATGGA GGTCCACCTC ACGGTGGCTG TGCTCGTGTA CCCCACCCGA
    GATGGCCAAG GTCCTTTGCA CCATGGTGTC TGTAGCACAT GGCTCAGCAA CCTGAAGCCC
    CAAGACCCAG TACCCTGCTT TGTACGAAGT GCCGGCAACT TCAAGCTCCC CGAGGATCCC
    TCCCGTCCTT GCATCCTCAT TGGACCTGGC ACAGGCATCG CCCCCTTCCG CAGTTTCTGG
    CAGCAGCGGC TCCATGACAT CAAGCACAAA GGGCTCCGAG GCAGCCGCAT GACCCTGGTG
    TTTGGGTGCC GCCGCCCAGA TGAGGACCAC CTGTATCGGG AGGAGATGTT GGAGATGGCC
    CAGAGTGGGG TGCTGCATGA GGTGCACACA GCCTATTCTC GCCTGCCTGG CCAGCCCAAG
    GTCTATGTTC AAGACATCCT GCGGCAGCAG CTGGCCAGCC AGGTGCTCCG CATGCTCCAT
    GAGGAGCAGG GCCACCTTTA TGTCTGTGGG GATGTGCGTA TGGCCCGGGA TGTGGCCCAT
    ACCCTGAAGC ACCTGGTGGC TGCCAAGCTG AGCCTGAGTG AAGAGCAAGT TGAGGACTAT
    TTTTTCCAGC TTAAGAGCCA GAAGCGCTAT CATGAAGATA TCTTTGGTGC TGTGTTTCCC
    TATGAGGTGA AAAAAGATGG TGCAGCAAAA CAGCCCAGCG ATCCCAGAGT TCCAGCAGCC
    CACGGAAGAA GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.