NOS2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOS2
Entrez Gene ID	4843
Full Name	nitric oxide synthase 2
Synonyms	HEP-NOS,INOS,NOS,NOS2A
General protein information	Preferred Names nitric oxide synthase 2 Names nitric oxide synthase, inducible NOS, type II hepatocyte NOS inducible NO synthase inducible NOS nitric oxide synthase 2, inducible nitric oxide synthase 2A (inducible, hepatocytes) nitric oxide synthase, macrophage peptidyl-cysteine S-nitrosylase NOS2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q11.2
Summary	Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008].
Disorder MIM:	163730
Disorder Html:	{Hypertension, susceptibility to}, 145500 (2); {Malaria, resistance to}, 611162 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011524862.1	XP_011523164.1	nitric oxide synthase, inducible isoform X4
XM_011524859.2	XP_011523161.1	nitric oxide synthase, inducible isoform X1
XM_011524861.1	XP_011523163.1	nitric oxide synthase, inducible isoform X3
XM_011524860.2	XP_011523162.1	nitric oxide synthase, inducible isoform X2
NM_000625.4	NP_000616.3	nitric oxide synthase, inducible

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NOS2	NP_001070267.1
Canis lupus familiaris (dog)	NOS2	XP_005624903.1
Xenopus tropicalis (tropical clawed frog)	nos2	XP_002935342.2
	NOS2	XP_001106245.1
Rattus norvegicus (Norway rat)	Nos2	NP_036743.3
Danio rerio (zebrafish)	nos2a	NP_001098407.1
Homo sapiens (human)	NOS2	NP_000616.3
Rattus norvegicus (Norway rat)	LOC497963	XP_006247011.1
Gallus gallus (chicken)	NOS2	NP_990292.1
Pan troglodytes (chimpanzee)	NOS2	XP_001148238.1
Mus musculus (house mouse)	Nos2	NP_035057.1
Danio rerio (zebrafish)	nos2b	NP_001106973.1

Gene Ontology

The following NOS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu59290	XM_011524862.1 Latest version!	Homo sapiens nitric oxide synthase 2 (NOS2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu28036	NM_000625.4 Latest version!	Homo sapiens nitric oxide synthase 2 (NOS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
OHu28036	XM_011524859.2 Latest version!	Homo sapiens nitric oxide synthase 2 (NOS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
OHu59288	XM_011524860.2 Latest version!	Homo sapiens nitric oxide synthase 2 (NOS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu59289	XM_011524861.1 Latest version!	Homo sapiens nitric oxide synthase 2 (NOS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOS2 ( XM_011524862.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523164.1 Homo sapiens nitric oxide synthase 2 (NOS2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu59290
Clone ID Related Accession (Same CDS sequence)	XM_011524862.1
Accession Version	XM_011524862.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nitric oxide synthase, inducible isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGAAC ACATCTGCAG ACACGTGCGT TACTCCACCA ACAATGGCAA CATCAGGTCG 
GCCATCACCG TGTTCCCCCA GCGGAGTGAT GGCAAGCACG ACTTCCGGGT GTGGAATGCT 
CAGCTCATCC GCTATGCTGG CTACCAGATG CCAGATGGCA GCATCAGAGG GGACCCTGCC 
AACGTGGAAT TCACTCAGCT GTGCATCGAC CTGGGCTGGA AGCCCAAGTA CGGCCGCTTC 
GATGTGGTCC CCCTGGTCCT GCAGGCCAAT GGCCGTGACC CTGAGCTCTT CGAAATCCCA 
CCTGACCTTG TGCTTGAGGT GGCCATGGAA CATCCCAAAT ACGAGTGGTT TCGGGAACTG 
GAGCTAAAGT GGTACGCCCT GCCTGCAGTG GCCAACATGC TGCTTGAGGT GGGCGGCCTG 
GAGTTCCCAG GGTGCCCCTT CAATGGCTGG TACATGGGCA CAGAGATCGG AGTCCGGGAC 
TTCTGTGACG TCCAGCGCTA CAACATCCTG GAGGAAGTGG GCAGGAGAAT GGGCCTGGAA 
ACGCACAAGC TGGCCTCGCT CTGGAAAGAC CAGGCTGTCG TTGAGATCAA CATTGCTGTG 
CTCCATAGTT TCCAGAAGCA GAATGTGACC ATCATGGACC ACCACTCGGC TGCAGAATCC 
TTCATGAAGT ACATGCAGAA TGAATACCGG TCCCGTGGGG GCTGCCCGGC AGACTGGATT 
TGGCTGGTCC CTCCCATGTC TGGGAGCATC ACCCCCGTGT TTCACCAGGA GATGCTGAAC 
TACGTCCTGT CCCCTTTCTA CTACTATCAG GTAGAGGCCT GGAAAACCCA TGTCTGGCAG 
GACGAGAAGC GGAGACCCAA GAGAAGAGAG ATTCCATTGA AAGTCTTGGT CAAAGCTGTG 
CTCTTTGCCT GTATGCTGAT GCGCAAGACA ATGGCGTCCC GAGTCAGAGT CACCATCCTC 
TTTGCGACAG AGACAGGAAA ATCAGAGGCG CTGGCCTGGG ACCTGGGGGC CTTATTCAGC 
TGTGCCTTCA ACCCCAAGGT TGTCTGCATG GATAAGTACA GGCTGAGCTG CCTGGAGGAG 
GAACGGCTGC TGTTGGTGGT GACCAGTACG TTTGGCAATG GAGACTGCCC TGGCAATGGA 
GAGAAACTGA AGAAATCGCT CTTCATGCTG AAAGAGCTCA ACAACAAATT CAGGTACGCT 
GTGTTTGGCC TCGGCTCCAG CATGTACCCT CGGTTCTGCG CCTTTGCTCA TGACATTGAT 
CAGAAGCTGT CCCACCTGGG GGCCTCTCAG CTCACCCCGA TGGGAGAAGG GGATGAGCTC 
AGTGGGCAGG AGGACGCCTT CCGCAGCTGG GCCGTGCAAA CCTTCAAGGC AGCCTGTGAG 
ACGTTTGATG TCCGAGGCAA ACAGCACATT CAGATCCCCA AGCTCTACAC CTCCAATGTG 
ACCTGGGACC CGCACCACTA CAGGCTCGTG CAGGACTCAC AGCCTTTGGA CCTCAGCAAA 
GCCCTCAGCA GCATGCATGC CAAGAACGTG TTCACCATGA GGCTCAAATC TCGGCAGAAT 
CTACAAAGTC CGACATCCAG CCGTGCCACC ATCCTGGTGG AACTCTCCTG TGAGGATGGC 
CAAGGCCTGA ACTACCTGCC GGGGGAGCAC CTTGGGGTTT GCCCAGGCAA CCAGCCGGCC 
CTGGTCCAAG GTATCCTGGA GCGAGTGGTG GATGGCCCCA CACCCCACCA GACAGTGCGC 
CTGGAGGCCC TGGATGAGAG TGGCAGCTAC TGGGTCAGTG ACAAGAGGCT GCCCCCCTGC 
TCACTCAGCC AGGCCCTCAC CTACTTCCTG GACATCACCA CACCCCCAAC CCAGCTGCTG 
CTCCAAAAGC TGGCCCAGGT GGCCACAGAA GAGCCTGAGA GACAGAGGCT GGAGGCCCTG 
TGCCAGCCCT CAGAGTACAG CAAGTGGAAG TTCACCAACA GCCCCACATT CCTGGAGGTG 
CTAGAGGAGT TCCCGTCCCT GCGGGTGTCT GCTGGCTTCC TGCTTTCCCA GCTCCCCATT 
CTGAAGCCCA GGTTCTACTC CATCAGCTCC TCCCGGGATC ACACGCCCAC AGAGATCCAC 
CTGACTGTGG CCGTGGTCAC CTACCACACC CGAGATGGCC AGGGTCCCCT GCACCACGGC 
GTCTGCAGCA CATGGCTCAA CAGCCTGAAG CCCCAAGACC CAGTGCCCTG CTTTGTGCGG 
AATGCCAGCG GCTTCCACCT CCCCGAGGAT CCCTCCCATC CTTGCATCCT CATCGGGCCT 
GGCACAGGCA TCGCGCCCTT CCGCAGTTTC TGGCAGCAAC GGCTCCATGA CTCCCAGCAC 
AAGGGAGTGC GGGGAGGCCG CATGACCTTG GTGTTTGGGT GCCGCCGCCC AGATGAGGAC 
CACATCTACC AGGAGGAGAT GCTGGAGATG GCCCAGAAGG GGGTGCTGCA TGCGGTGCAC 
ACAGCCTATT CCCGCCTGCC TGGCAAGCCC AAGGTCTATG TTCAGGACAT CCTGCGGCAG 
CAGCTGGCCA GCGAGGTGCT CCGTGTGCTC CACAAGGAGC CAGGCCACCT CTATGTTTGC 
GGGGATGTGC GCATGGCCCG GGACGTGGCC CACACCCTGA AGCAGCTGGT GGCTGCCAAG 
CTGAAATTGA ATGAGGAGCA GGTCGAGGAC TATTTCTTTC AGCTCAAGAG CCAGAAGCGC 
TATCACGAAG ATATCTTTGG TGCTGTATTT CCTTACGAGG CGAAGAAGGA CAGGGTGGCG 
GTGCAGCCCA GCAGCCTGGA GATGTCAGCG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523164.1
CDS	528..3323
Translation	MFEHICRHVRYSTNNGNIRSAITVFPQRSDGKHDFRVWNAQLIRYAGYQMPDGSIRGDPANVEFTQLCIDLGWKPKYGRFDVVPLVLQANGRDPELFEIPPDLVLEVAMEHPKYEWFRELELKWYALPAVANMLLEVGGLEFPGCPFNGWYMGTEIGVRDFCDVQRYNILEEVGRRMGLETHKLASLWKDQAVVEINIAVLHSFQKQNVTIMDHHSAAESFMKYMQNEYRSRGGCPADWIWLVPPMSGSITPVFHQEMLNYVLSPFYYYQVEAWKTHVWQDEKRRPKRREIPLKVLVKAVLFACMLMRKTMASRVRVTILFATETGKSEALAWDLGALFSCAFNPKVVCMDKYRLSCLEEERLLLVVTSTFGNGDCPGNGEKLKKSLFMLKELNNKFRYAVFGLGSSMYPRFCAFAHDIDQKLSHLGASQLTPMGEGDELSGQEDAFRSWAVQTFKAACETFDVRGKQHIQIPKLYTSNVTWDPHHYRLVQDSQPLDLSKALSSMHAKNVFTMRLKSRQNLQSPTSSRATILVELSCEDGQGLNYLPGEHLGVCPGNQPALVQGILERVVDGPTPHQTVRLEALDESGSYWVSDKRLPPCSLSQALTYFLDITTPPTQLLLQKLAQVATEEPERQRLEALCQPSEYSKWKFTNSPTFLEVLEEFPSLRVSAGFLLSQLPILKPRFYSISSSRDHTPTEIHLTVAVVTYHTRDGQGPLHHGVCSTWLNSLKPQDPVPCFVRNASGFHLPEDPSHPCILIGPGTGIAPFRSFWQQRLHDSQHKGVRGGRMTLVFGCRRPDEDHIYQEEMLEMAQKGVLHAVHTAYSRLPGKPKVYVQDILRQQLASEVLRVLHKEPGHLYVCGDVRMARDVAHTLKQLVAAKLKLNEEQVEDYFFQLKSQKRYHEDIFGAVFPYEAKKDRVAVQPSSLEMSAL

Target ORF information:

RefSeq Version	XM_011524862.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide synthase 2 (NOS2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011524862.1

ORF Insert Sequence:

ATGTTTGAAC ACATCTGCAG ACACGTGCGT TACTCCACCA ACAATGGCAA CATCAGGTCG 
GCCATCACCG TGTTCCCCCA GCGGAGTGAT GGCAAGCACG ACTTCCGGGT GTGGAATGCT 
CAGCTCATCC GCTATGCTGG CTACCAGATG CCAGATGGCA GCATCAGAGG GGACCCTGCC 
AACGTGGAAT TCACTCAGCT GTGCATCGAC CTGGGCTGGA AGCCCAAGTA CGGCCGCTTC 
GATGTGGTCC CCCTGGTCCT GCAGGCCAAT GGCCGTGACC CTGAGCTCTT CGAAATCCCA 
CCTGACCTTG TGCTTGAGGT GGCCATGGAA CATCCCAAAT ACGAGTGGTT TCGGGAACTG 
GAGCTAAAGT GGTACGCCCT GCCTGCAGTG GCCAACATGC TGCTTGAGGT GGGCGGCCTG 
GAGTTCCCAG GGTGCCCCTT CAATGGCTGG TACATGGGCA CAGAGATCGG AGTCCGGGAC 
TTCTGTGACG TCCAGCGCTA CAACATCCTG GAGGAAGTGG GCAGGAGAAT GGGCCTGGAA 
ACGCACAAGC TGGCCTCGCT CTGGAAAGAC CAGGCTGTCG TTGAGATCAA CATTGCTGTG 
CTCCATAGTT TCCAGAAGCA GAATGTGACC ATCATGGACC ACCACTCGGC TGCAGAATCC 
TTCATGAAGT ACATGCAGAA TGAATACCGG TCCCGTGGGG GCTGCCCGGC AGACTGGATT 
TGGCTGGTCC CTCCCATGTC TGGGAGCATC ACCCCCGTGT TTCACCAGGA GATGCTGAAC 
TACGTCCTGT CCCCTTTCTA CTACTATCAG GTAGAGGCCT GGAAAACCCA TGTCTGGCAG 
GACGAGAAGC GGAGACCCAA GAGAAGAGAG ATTCCATTGA AAGTCTTGGT CAAAGCTGTG 
CTCTTTGCCT GTATGCTGAT GCGCAAGACA ATGGCGTCCC GAGTCAGAGT CACCATCCTC 
TTTGCGACAG AGACAGGAAA ATCAGAGGCG CTGGCCTGGG ACCTGGGGGC CTTATTCAGC 
TGTGCCTTCA ACCCCAAGGT TGTCTGCATG GATAAGTACA GGCTGAGCTG CCTGGAGGAG 
GAACGGCTGC TGTTGGTGGT GACCAGTACG TTTGGCAATG GAGACTGCCC TGGCAATGGA 
GAGAAACTGA AGAAATCGCT CTTCATGCTG AAAGAGCTCA ACAACAAATT CAGGTACGCT 
GTGTTTGGCC TCGGCTCCAG CATGTACCCT CGGTTCTGCG CCTTTGCTCA TGACATTGAT 
CAGAAGCTGT CCCACCTGGG GGCCTCTCAG CTCACCCCGA TGGGAGAAGG GGATGAGCTC 
AGTGGGCAGG AGGACGCCTT CCGCAGCTGG GCCGTGCAAA CCTTCAAGGC AGCCTGTGAG 
ACGTTTGATG TCCGAGGCAA ACAGCACATT CAGATCCCCA AGCTCTACAC CTCCAATGTG 
ACCTGGGACC CGCACCACTA CAGGCTCGTG CAGGACTCAC AGCCTTTGGA CCTCAGCAAA 
GCCCTCAGCA GCATGCATGC CAAGAACGTG TTCACCATGA GGCTCAAATC TCGGCAGAAT 
CTACAAAGTC CGACATCCAG CCGTGCCACC ATCCTGGTGG AACTCTCCTG TGAGGATGGC 
CAAGGCCTGA ACTACCTGCC GGGGGAGCAC CTTGGGGTTT GCCCAGGCAA CCAGCCGGCC 
CTGGTCCAAG GTATCCTGGA GCGAGTGGTG GATGGCCCCA CACCCCACCA GACAGTGCGC 
CTGGAGGCCC TGGATGAGAG TGGCAGCTAC TGGGTCAGTG ACAAGAGGCT GCCCCCCTGC 
TCACTCAGCC AGGCCCTCAC CTACTTCCTG GACATCACCA CACCCCCAAC CCAGCTGCTG 
CTCCAAAAGC TGGCCCAGGT GGCCACAGAA GAGCCTGAGA GACAGAGGCT GGAGGCCCTG 
TGCCAGCCCT CAGAGTACAG CAAGTGGAAG TTCACCAACA GCCCCACATT CCTGGAGGTG 
CTAGAGGAGT TCCCGTCCCT GCGGGTGTCT GCTGGCTTCC TGCTTTCCCA GCTCCCCATT 
CTGAAGCCCA GGTTCTACTC CATCAGCTCC TCCCGGGATC ACACGCCCAC AGAGATCCAC 
CTGACTGTGG CCGTGGTCAC CTACCACACC CGAGATGGCC AGGGTCCCCT GCACCACGGC 
GTCTGCAGCA CATGGCTCAA CAGCCTGAAG CCCCAAGACC CAGTGCCCTG CTTTGTGCGG 
AATGCCAGCG GCTTCCACCT CCCCGAGGAT CCCTCCCATC CTTGCATCCT CATCGGGCCT 
GGCACAGGCA TCGCGCCCTT CCGCAGTTTC TGGCAGCAAC GGCTCCATGA CTCCCAGCAC 
AAGGGAGTGC GGGGAGGCCG CATGACCTTG GTGTTTGGGT GCCGCCGCCC AGATGAGGAC 
CACATCTACC AGGAGGAGAT GCTGGAGATG GCCCAGAAGG GGGTGCTGCA TGCGGTGCAC 
ACAGCCTATT CCCGCCTGCC TGGCAAGCCC AAGGTCTATG TTCAGGACAT CCTGCGGCAG 
CAGCTGGCCA GCGAGGTGCT CCGTGTGCTC CACAAGGAGC CAGGCCACCT CTATGTTTGC 
GGGGATGTGC GCATGGCCCG GGACGTGGCC CACACCCTGA AGCAGCTGGT GGCTGCCAAG 
CTGAAATTGA ATGAGGAGCA GGTCGAGGAC TATTTCTTTC AGCTCAAGAG CCAGAAGCGC 
TATCACGAAG ATATCTTTGG TGCTGTATTT CCTTACGAGG CGAAGAAGGA CAGGGTGGCG 
GTGCAGCCCA GCAGCCTGGA GATGTCAGCG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS2 ( NM_000625.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000616.3 Homo sapiens nitric oxide synthase 2 (NOS2), mRNA.

Price & Availability↑

CloneID	OHu28036
Clone ID Related Accession (Same CDS sequence)	XM_011524859.2 , NM_000625.4
Accession Version	NM_000625.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	nitric oxide synthase, inducible
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC130289.11. This sequence is a reference standard in the RefSeqGene project. On Sep 25, 2008 this sequence version replaced NM_000625.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U31511.1, X73029.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000313735.11/ ENSP00000327251.6 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCTGTC CTTGGAAATT TCTGTTCAAG ACCAAATTCC ACCAGTATGC AATGAATGGG 
GAAAAAGACA TCAACAACAA TGTGGAGAAA GCCCCCTGTG CCACCTCCAG TCCAGTGACA 
CAGGATGACC TTCAGTATCA CAACCTCAGC AAGCAGCAGA ATGAGTCCCC GCAGCCCCTC 
GTGGAGACGG GAAAGAAGTC TCCAGAATCT CTGGTCAAGC TGGATGCAAC CCCATTGTCC 
TCCCCACGGC ATGTGAGGAT CAAAAACTGG GGCAGCGGGA TGACTTTCCA AGACACACTT 
CACCATAAGG CCAAAGGGAT TTTAACTTGC AGGTCCAAAT CTTGCCTGGG GTCCATTATG 
ACTCCCAAAA GTTTGACCAG AGGACCCAGG GACAAGCCTA CCCCTCCAGA TGAGCTTCTA 
CCTCAAGCTA TCGAATTTGT CAACCAATAT TACGGCTCCT TCAAAGAGGC AAAAATAGAG 
GAACATCTGG CCAGGGTGGA AGCGGTAACA AAGGAGATAG AAACAACAGG AACCTACCAA 
CTGACGGGAG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAATGC CCCACGCTGC 
ATTGGGAGGA TCCAGTGGTC CAACCTGCAG GTCTTCGATG CCCGCAGCTG TTCCACTGCC 
CGGGAAATGT TTGAACACAT CTGCAGACAC GTGCGTTACT CCACCAACAA TGGCAACATC 
AGGTCGGCCA TCACCGTGTT CCCCCAGCGG AGTGATGGCA AGCACGACTT CCGGGTGTGG 
AATGCTCAGC TCATCCGCTA TGCTGGCTAC CAGATGCCAG ATGGCAGCAT CAGAGGGGAC 
CCTGCCAACG TGGAATTCAC TCAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC 
CGCTTCGATG TGGTCCCCCT GGTCCTGCAG GCCAATGGCC GTGACCCTGA GCTCTTCGAA 
ATCCCACCTG ACCTTGTGCT TGAGGTGGCC ATGGAACATC CCAAATACGA GTGGTTTCGG 
GAACTGGAGC TAAAGTGGTA CGCCCTGCCT GCAGTGGCCA ACATGCTGCT TGAGGTGGGC 
GGCCTGGAGT TCCCAGGGTG CCCCTTCAAT GGCTGGTACA TGGGCACAGA GATCGGAGTC 
CGGGACTTCT GTGACGTCCA GCGCTACAAC ATCCTGGAGG AAGTGGGCAG GAGAATGGGC 
CTGGAAACGC ACAAGCTGGC CTCGCTCTGG AAAGACCAGG CTGTCGTTGA GATCAACATT 
GCTGTGCTCC ATAGTTTCCA GAAGCAGAAT GTGACCATCA TGGACCACCA CTCGGCTGCA 
GAATCCTTCA TGAAGTACAT GCAGAATGAA TACCGGTCCC GTGGGGGCTG CCCGGCAGAC 
TGGATTTGGC TGGTCCCTCC CATGTCTGGG AGCATCACCC CCGTGTTTCA CCAGGAGATG 
CTGAACTACG TCCTGTCCCC TTTCTACTAC TATCAGGTAG AGGCCTGGAA AACCCATGTC 
TGGCAGGACG AGAAGCGGAG ACCCAAGAGA AGAGAGATTC CATTGAAAGT CTTGGTCAAA 
GCTGTGCTCT TTGCCTGTAT GCTGATGCGC AAGACAATGG CGTCCCGAGT CAGAGTCACC 
ATCCTCTTTG CGACAGAGAC AGGAAAATCA GAGGCGCTGG CCTGGGACCT GGGGGCCTTA 
TTCAGCTGTG CCTTCAACCC CAAGGTTGTC TGCATGGATA AGTACAGGCT GAGCTGCCTG 
GAGGAGGAAC GGCTGCTGTT GGTGGTGACC AGTACGTTTG GCAATGGAGA CTGCCCTGGC 
AATGGAGAGA AACTGAAGAA ATCGCTCTTC ATGCTGAAAG AGCTCAACAA CAAATTCAGG 
TACGCTGTGT TTGGCCTCGG CTCCAGCATG TACCCTCGGT TCTGCGCCTT TGCTCATGAC 
ATTGATCAGA AGCTGTCCCA CCTGGGGGCC TCTCAGCTCA CCCCGATGGG AGAAGGGGAT 
GAGCTCAGTG GGCAGGAGGA CGCCTTCCGC AGCTGGGCCG TGCAAACCTT CAAGGCAGCC 
TGTGAGACGT TTGATGTCCG AGGCAAACAG CACATTCAGA TCCCCAAGCT CTACACCTCC 
AATGTGACCT GGGACCCGCA CCACTACAGG CTCGTGCAGG ACTCACAGCC TTTGGACCTC 
AGCAAAGCCC TCAGCAGCAT GCATGCCAAG AACGTGTTCA CCATGAGGCT CAAATCTCGG 
CAGAATCTAC AAAGTCCGAC ATCCAGCCGT GCCACCATCC TGGTGGAACT CTCCTGTGAG 
GATGGCCAAG GCCTGAACTA CCTGCCGGGG GAGCACCTTG GGGTTTGCCC AGGCAACCAG 
CCGGCCCTGG TCCAAGGTAT CCTGGAGCGA GTGGTGGATG GCCCCACACC CCACCAGACA 
GTGCGCCTGG AGGCCCTGGA TGAGAGTGGC AGCTACTGGG TCAGTGACAA GAGGCTGCCC 
CCCTGCTCAC TCAGCCAGGC CCTCACCTAC TTCCTGGACA TCACCACACC CCCAACCCAG 
CTGCTGCTCC AAAAGCTGGC CCAGGTGGCC ACAGAAGAGC CTGAGAGACA GAGGCTGGAG 
GCCCTGTGCC AGCCCTCAGA GTACAGCAAG TGGAAGTTCA CCAACAGCCC CACATTCCTG 
GAGGTGCTAG AGGAGTTCCC GTCCCTGCGG GTGTCTGCTG GCTTCCTGCT TTCCCAGCTC 
CCCATTCTGA AGCCCAGGTT CTACTCCATC AGCTCCTCCC GGGATCACAC GCCCACAGAG 
ATCCACCTGA CTGTGGCCGT GGTCACCTAC CACACCCGAG ATGGCCAGGG TCCCCTGCAC 
CACGGCGTCT GCAGCACATG GCTCAACAGC CTGAAGCCCC AAGACCCAGT GCCCTGCTTT 
GTGCGGAATG CCAGCGGCTT CCACCTCCCC GAGGATCCCT CCCATCCTTG CATCCTCATC 
GGGCCTGGCA CAGGCATCGC GCCCTTCCGC AGTTTCTGGC AGCAACGGCT CCATGACTCC 
CAGCACAAGG GAGTGCGGGG AGGCCGCATG ACCTTGGTGT TTGGGTGCCG CCGCCCAGAT 
GAGGACCACA TCTACCAGGA GGAGATGCTG GAGATGGCCC AGAAGGGGGT GCTGCATGCG 
GTGCACACAG CCTATTCCCG CCTGCCTGGC AAGCCCAAGG TCTATGTTCA GGACATCCTG 
CGGCAGCAGC TGGCCAGCGA GGTGCTCCGT GTGCTCCACA AGGAGCCAGG CCACCTCTAT 
GTTTGCGGGG ATGTGCGCAT GGCCCGGGAC GTGGCCCACA CCCTGAAGCA GCTGGTGGCT 
GCCAAGCTGA AATTGAATGA GGAGCAGGTC GAGGACTATT TCTTTCAGCT CAAGAGCCAG 
AAGCGCTATC ACGAAGATAT CTTTGGTGCT GTATTTCCTT ACGAGGCGAA GAAGGACAGG 
GTGGCGGTGC AGCCCAGCAG CCTGGAGATG TCAGCGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000616.3
CDS	265..3726
Translation	MACPWKFLFKTKFHQYAMNGEKDINNNVEKAPCATSSPVTQDDLQYHNLSKQQNESPQPLVETGKKSPESLVKLDATPLSSPRHVRIKNWGSGMTFQDTLHHKAKGILTCRSKSCLGSIMTPKSLTRGPRDKPTPPDELLPQAIEFVNQYYGSFKEAKIEEHLARVEAVTKEIETTGTYQLTGDELIFATKQAWRNAPRCIGRIQWSNLQVFDARSCSTAREMFEHICRHVRYSTNNGNIRSAITVFPQRSDGKHDFRVWNAQLIRYAGYQMPDGSIRGDPANVEFTQLCIDLGWKPKYGRFDVVPLVLQANGRDPELFEIPPDLVLEVAMEHPKYEWFRELELKWYALPAVANMLLEVGGLEFPGCPFNGWYMGTEIGVRDFCDVQRYNILEEVGRRMGLETHKLASLWKDQAVVEINIAVLHSFQKQNVTIMDHHSAAESFMKYMQNEYRSRGGCPADWIWLVPPMSGSITPVFHQEMLNYVLSPFYYYQVEAWKTHVWQDEKRRPKRREIPLKVLVKAVLFACMLMRKTMASRVRVTILFATETGKSEALAWDLGALFSCAFNPKVVCMDKYRLSCLEEERLLLVVTSTFGNGDCPGNGEKLKKSLFMLKELNNKFRYAVFGLGSSMYPRFCAFAHDIDQKLSHLGASQLTPMGEGDELSGQEDAFRSWAVQTFKAACETFDVRGKQHIQIPKLYTSNVTWDPHHYRLVQDSQPLDLSKALSSMHAKNVFTMRLKSRQNLQSPTSSRATILVELSCEDGQGLNYLPGEHLGVCPGNQPALVQGILERVVDGPTPHQTVRLEALDESGSYWVSDKRLPPCSLSQALTYFLDITTPPTQLLLQKLAQVATEEPERQRLEALCQPSEYSKWKFTNSPTFLEVLEEFPSLRVSAGFLLSQLPILKPRFYSISSSRDHTPTEIHLTVAVVTYHTRDGQGPLHHGVCSTWLNSLKPQDPVPCFVRNASGFHLPEDPSHPCILIGPGTGIAPFRSFWQQRLHDSQHKGVRGGRMTLVFGCRRPDEDHIYQEEMLEMAQKGVLHAVHTAYSRLPGKPKVYVQDILRQQLASEVLRVLHKEPGHLYVCGDVRMARDVAHTLKQLVAAKLKLNEEQVEDYFFQLKSQKRYHEDIFGAVFPYEAKKDRVAVQPSSLEMSAL

Target ORF information:

RefSeq Version	NM_000625.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide synthase 2 (NOS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000625.4

ORF Insert Sequence:

ATGGCCTGTC CTTGGAAATT TCTGTTCAAG ACCAAATTCC ACCAGTATGC AATGAATGGG 
GAAAAAGACA TCAACAACAA TGTGGAGAAA GCCCCCTGTG CCACCTCCAG TCCAGTGACA 
CAGGATGACC TTCAGTATCA CAACCTCAGC AAGCAGCAGA ATGAGTCCCC GCAGCCCCTC 
GTGGAGACGG GAAAGAAGTC TCCAGAATCT CTGGTCAAGC TGGATGCAAC CCCATTGTCC 
TCCCCACGGC ATGTGAGGAT CAAAAACTGG GGCAGCGGGA TGACTTTCCA AGACACACTT 
CACCATAAGG CCAAAGGGAT TTTAACTTGC AGGTCCAAAT CTTGCCTGGG GTCCATTATG 
ACTCCCAAAA GTTTGACCAG AGGACCCAGG GACAAGCCTA CCCCTCCAGA TGAGCTTCTA 
CCTCAAGCTA TCGAATTTGT CAACCAATAT TACGGCTCCT TCAAAGAGGC AAAAATAGAG 
GAACATCTGG CCAGGGTGGA AGCGGTAACA AAGGAGATAG AAACAACAGG AACCTACCAA 
CTGACGGGAG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAATGC CCCACGCTGC 
ATTGGGAGGA TCCAGTGGTC CAACCTGCAG GTCTTCGATG CCCGCAGCTG TTCCACTGCC 
CGGGAAATGT TTGAACACAT CTGCAGACAC GTGCGTTACT CCACCAACAA TGGCAACATC 
AGGTCGGCCA TCACCGTGTT CCCCCAGCGG AGTGATGGCA AGCACGACTT CCGGGTGTGG 
AATGCTCAGC TCATCCGCTA TGCTGGCTAC CAGATGCCAG ATGGCAGCAT CAGAGGGGAC 
CCTGCCAACG TGGAATTCAC TCAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC 
CGCTTCGATG TGGTCCCCCT GGTCCTGCAG GCCAATGGCC GTGACCCTGA GCTCTTCGAA 
ATCCCACCTG ACCTTGTGCT TGAGGTGGCC ATGGAACATC CCAAATACGA GTGGTTTCGG 
GAACTGGAGC TAAAGTGGTA CGCCCTGCCT GCAGTGGCCA ACATGCTGCT TGAGGTGGGC 
GGCCTGGAGT TCCCAGGGTG CCCCTTCAAT GGCTGGTACA TGGGCACAGA GATCGGAGTC 
CGGGACTTCT GTGACGTCCA GCGCTACAAC ATCCTGGAGG AAGTGGGCAG GAGAATGGGC 
CTGGAAACGC ACAAGCTGGC CTCGCTCTGG AAAGACCAGG CTGTCGTTGA GATCAACATT 
GCTGTGCTCC ATAGTTTCCA GAAGCAGAAT GTGACCATCA TGGACCACCA CTCGGCTGCA 
GAATCCTTCA TGAAGTACAT GCAGAATGAA TACCGGTCCC GTGGGGGCTG CCCGGCAGAC 
TGGATTTGGC TGGTCCCTCC CATGTCTGGG AGCATCACCC CCGTGTTTCA CCAGGAGATG 
CTGAACTACG TCCTGTCCCC TTTCTACTAC TATCAGGTAG AGGCCTGGAA AACCCATGTC 
TGGCAGGACG AGAAGCGGAG ACCCAAGAGA AGAGAGATTC CATTGAAAGT CTTGGTCAAA 
GCTGTGCTCT TTGCCTGTAT GCTGATGCGC AAGACAATGG CGTCCCGAGT CAGAGTCACC 
ATCCTCTTTG CGACAGAGAC AGGAAAATCA GAGGCGCTGG CCTGGGACCT GGGGGCCTTA 
TTCAGCTGTG CCTTCAACCC CAAGGTTGTC TGCATGGATA AGTACAGGCT GAGCTGCCTG 
GAGGAGGAAC GGCTGCTGTT GGTGGTGACC AGTACGTTTG GCAATGGAGA CTGCCCTGGC 
AATGGAGAGA AACTGAAGAA ATCGCTCTTC ATGCTGAAAG AGCTCAACAA CAAATTCAGG 
TACGCTGTGT TTGGCCTCGG CTCCAGCATG TACCCTCGGT TCTGCGCCTT TGCTCATGAC 
ATTGATCAGA AGCTGTCCCA CCTGGGGGCC TCTCAGCTCA CCCCGATGGG AGAAGGGGAT 
GAGCTCAGTG GGCAGGAGGA CGCCTTCCGC AGCTGGGCCG TGCAAACCTT CAAGGCAGCC 
TGTGAGACGT TTGATGTCCG AGGCAAACAG CACATTCAGA TCCCCAAGCT CTACACCTCC 
AATGTGACCT GGGACCCGCA CCACTACAGG CTCGTGCAGG ACTCACAGCC TTTGGACCTC 
AGCAAAGCCC TCAGCAGCAT GCATGCCAAG AACGTGTTCA CCATGAGGCT CAAATCTCGG 
CAGAATCTAC AAAGTCCGAC ATCCAGCCGT GCCACCATCC TGGTGGAACT CTCCTGTGAG 
GATGGCCAAG GCCTGAACTA CCTGCCGGGG GAGCACCTTG GGGTTTGCCC AGGCAACCAG 
CCGGCCCTGG TCCAAGGTAT CCTGGAGCGA GTGGTGGATG GCCCCACACC CCACCAGACA 
GTGCGCCTGG AGGCCCTGGA TGAGAGTGGC AGCTACTGGG TCAGTGACAA GAGGCTGCCC 
CCCTGCTCAC TCAGCCAGGC CCTCACCTAC TTCCTGGACA TCACCACACC CCCAACCCAG 
CTGCTGCTCC AAAAGCTGGC CCAGGTGGCC ACAGAAGAGC CTGAGAGACA GAGGCTGGAG 
GCCCTGTGCC AGCCCTCAGA GTACAGCAAG TGGAAGTTCA CCAACAGCCC CACATTCCTG 
GAGGTGCTAG AGGAGTTCCC GTCCCTGCGG GTGTCTGCTG GCTTCCTGCT TTCCCAGCTC 
CCCATTCTGA AGCCCAGGTT CTACTCCATC AGCTCCTCCC GGGATCACAC GCCCACAGAG 
ATCCACCTGA CTGTGGCCGT GGTCACCTAC CACACCCGAG ATGGCCAGGG TCCCCTGCAC 
CACGGCGTCT GCAGCACATG GCTCAACAGC CTGAAGCCCC AAGACCCAGT GCCCTGCTTT 
GTGCGGAATG CCAGCGGCTT CCACCTCCCC GAGGATCCCT CCCATCCTTG CATCCTCATC 
GGGCCTGGCA CAGGCATCGC GCCCTTCCGC AGTTTCTGGC AGCAACGGCT CCATGACTCC 
CAGCACAAGG GAGTGCGGGG AGGCCGCATG ACCTTGGTGT TTGGGTGCCG CCGCCCAGAT 
GAGGACCACA TCTACCAGGA GGAGATGCTG GAGATGGCCC AGAAGGGGGT GCTGCATGCG 
GTGCACACAG CCTATTCCCG CCTGCCTGGC AAGCCCAAGG TCTATGTTCA GGACATCCTG 
CGGCAGCAGC TGGCCAGCGA GGTGCTCCGT GTGCTCCACA AGGAGCCAGG CCACCTCTAT 
GTTTGCGGGG ATGTGCGCAT GGCCCGGGAC GTGGCCCACA CCCTGAAGCA GCTGGTGGCT 
GCCAAGCTGA AATTGAATGA GGAGCAGGTC GAGGACTATT TCTTTCAGCT CAAGAGCCAG 
AAGCGCTATC ACGAAGATAT CTTTGGTGCT GTATTTCCTT ACGAGGCGAA GAAGGACAGG 
GTGGCGGTGC AGCCCAGCAG CCTGGAGATG TCAGCGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS2 ( XM_011524859.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523161.1 Homo sapiens nitric oxide synthase 2 (NOS2), transcript variant X1, mRNA.

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CloneID	OHu28036
Clone ID Related Accession (Same CDS sequence)	XM_011524859.2 , NM_000625.4
Accession Version	XM_011524859.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nitric oxide synthase, inducible isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524859.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGTC CTTGGAAATT TCTGTTCAAG ACCAAATTCC ACCAGTATGC AATGAATGGG 
GAAAAAGACA TCAACAACAA TGTGGAGAAA GCCCCCTGTG CCACCTCCAG TCCAGTGACA 
CAGGATGACC TTCAGTATCA CAACCTCAGC AAGCAGCAGA ATGAGTCCCC GCAGCCCCTC 
GTGGAGACGG GAAAGAAGTC TCCAGAATCT CTGGTCAAGC TGGATGCAAC CCCATTGTCC 
TCCCCACGGC ATGTGAGGAT CAAAAACTGG GGCAGCGGGA TGACTTTCCA AGACACACTT 
CACCATAAGG CCAAAGGGAT TTTAACTTGC AGGTCCAAAT CTTGCCTGGG GTCCATTATG 
ACTCCCAAAA GTTTGACCAG AGGACCCAGG GACAAGCCTA CCCCTCCAGA TGAGCTTCTA 
CCTCAAGCTA TCGAATTTGT CAACCAATAT TACGGCTCCT TCAAAGAGGC AAAAATAGAG 
GAACATCTGG CCAGGGTGGA AGCGGTAACA AAGGAGATAG AAACAACAGG AACCTACCAA 
CTGACGGGAG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAATGC CCCACGCTGC 
ATTGGGAGGA TCCAGTGGTC CAACCTGCAG GTCTTCGATG CCCGCAGCTG TTCCACTGCC 
CGGGAAATGT TTGAACACAT CTGCAGACAC GTGCGTTACT CCACCAACAA TGGCAACATC 
AGGTCGGCCA TCACCGTGTT CCCCCAGCGG AGTGATGGCA AGCACGACTT CCGGGTGTGG 
AATGCTCAGC TCATCCGCTA TGCTGGCTAC CAGATGCCAG ATGGCAGCAT CAGAGGGGAC 
CCTGCCAACG TGGAATTCAC TCAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC 
CGCTTCGATG TGGTCCCCCT GGTCCTGCAG GCCAATGGCC GTGACCCTGA GCTCTTCGAA 
ATCCCACCTG ACCTTGTGCT TGAGGTGGCC ATGGAACATC CCAAATACGA GTGGTTTCGG 
GAACTGGAGC TAAAGTGGTA CGCCCTGCCT GCAGTGGCCA ACATGCTGCT TGAGGTGGGC 
GGCCTGGAGT TCCCAGGGTG CCCCTTCAAT GGCTGGTACA TGGGCACAGA GATCGGAGTC 
CGGGACTTCT GTGACGTCCA GCGCTACAAC ATCCTGGAGG AAGTGGGCAG GAGAATGGGC 
CTGGAAACGC ACAAGCTGGC CTCGCTCTGG AAAGACCAGG CTGTCGTTGA GATCAACATT 
GCTGTGCTCC ATAGTTTCCA GAAGCAGAAT GTGACCATCA TGGACCACCA CTCGGCTGCA 
GAATCCTTCA TGAAGTACAT GCAGAATGAA TACCGGTCCC GTGGGGGCTG CCCGGCAGAC 
TGGATTTGGC TGGTCCCTCC CATGTCTGGG AGCATCACCC CCGTGTTTCA CCAGGAGATG 
CTGAACTACG TCCTGTCCCC TTTCTACTAC TATCAGGTAG AGGCCTGGAA AACCCATGTC 
TGGCAGGACG AGAAGCGGAG ACCCAAGAGA AGAGAGATTC CATTGAAAGT CTTGGTCAAA 
GCTGTGCTCT TTGCCTGTAT GCTGATGCGC AAGACAATGG CGTCCCGAGT CAGAGTCACC 
ATCCTCTTTG CGACAGAGAC AGGAAAATCA GAGGCGCTGG CCTGGGACCT GGGGGCCTTA 
TTCAGCTGTG CCTTCAACCC CAAGGTTGTC TGCATGGATA AGTACAGGCT GAGCTGCCTG 
GAGGAGGAAC GGCTGCTGTT GGTGGTGACC AGTACGTTTG GCAATGGAGA CTGCCCTGGC 
AATGGAGAGA AACTGAAGAA ATCGCTCTTC ATGCTGAAAG AGCTCAACAA CAAATTCAGG 
TACGCTGTGT TTGGCCTCGG CTCCAGCATG TACCCTCGGT TCTGCGCCTT TGCTCATGAC 
ATTGATCAGA AGCTGTCCCA CCTGGGGGCC TCTCAGCTCA CCCCGATGGG AGAAGGGGAT 
GAGCTCAGTG GGCAGGAGGA CGCCTTCCGC AGCTGGGCCG TGCAAACCTT CAAGGCAGCC 
TGTGAGACGT TTGATGTCCG AGGCAAACAG CACATTCAGA TCCCCAAGCT CTACACCTCC 
AATGTGACCT GGGACCCGCA CCACTACAGG CTCGTGCAGG ACTCACAGCC TTTGGACCTC 
AGCAAAGCCC TCAGCAGCAT GCATGCCAAG AACGTGTTCA CCATGAGGCT CAAATCTCGG 
CAGAATCTAC AAAGTCCGAC ATCCAGCCGT GCCACCATCC TGGTGGAACT CTCCTGTGAG 
GATGGCCAAG GCCTGAACTA CCTGCCGGGG GAGCACCTTG GGGTTTGCCC AGGCAACCAG 
CCGGCCCTGG TCCAAGGTAT CCTGGAGCGA GTGGTGGATG GCCCCACACC CCACCAGACA 
GTGCGCCTGG AGGCCCTGGA TGAGAGTGGC AGCTACTGGG TCAGTGACAA GAGGCTGCCC 
CCCTGCTCAC TCAGCCAGGC CCTCACCTAC TTCCTGGACA TCACCACACC CCCAACCCAG 
CTGCTGCTCC AAAAGCTGGC CCAGGTGGCC ACAGAAGAGC CTGAGAGACA GAGGCTGGAG 
GCCCTGTGCC AGCCCTCAGA GTACAGCAAG TGGAAGTTCA CCAACAGCCC CACATTCCTG 
GAGGTGCTAG AGGAGTTCCC GTCCCTGCGG GTGTCTGCTG GCTTCCTGCT TTCCCAGCTC 
CCCATTCTGA AGCCCAGGTT CTACTCCATC AGCTCCTCCC GGGATCACAC GCCCACAGAG 
ATCCACCTGA CTGTGGCCGT GGTCACCTAC CACACCCGAG ATGGCCAGGG TCCCCTGCAC 
CACGGCGTCT GCAGCACATG GCTCAACAGC CTGAAGCCCC AAGACCCAGT GCCCTGCTTT 
GTGCGGAATG CCAGCGGCTT CCACCTCCCC GAGGATCCCT CCCATCCTTG CATCCTCATC 
GGGCCTGGCA CAGGCATCGC GCCCTTCCGC AGTTTCTGGC AGCAACGGCT CCATGACTCC 
CAGCACAAGG GAGTGCGGGG AGGCCGCATG ACCTTGGTGT TTGGGTGCCG CCGCCCAGAT 
GAGGACCACA TCTACCAGGA GGAGATGCTG GAGATGGCCC AGAAGGGGGT GCTGCATGCG 
GTGCACACAG CCTATTCCCG CCTGCCTGGC AAGCCCAAGG TCTATGTTCA GGACATCCTG 
CGGCAGCAGC TGGCCAGCGA GGTGCTCCGT GTGCTCCACA AGGAGCCAGG CCACCTCTAT 
GTTTGCGGGG ATGTGCGCAT GGCCCGGGAC GTGGCCCACA CCCTGAAGCA GCTGGTGGCT 
GCCAAGCTGA AATTGAATGA GGAGCAGGTC GAGGACTATT TCTTTCAGCT CAAGAGCCAG 
AAGCGCTATC ACGAAGATAT CTTTGGTGCT GTATTTCCTT ACGAGGCGAA GAAGGACAGG 
GTGGCGGTGC AGCCCAGCAG CCTGGAGATG TCAGCGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523161.1
CDS	291..3752
Translation	MACPWKFLFKTKFHQYAMNGEKDINNNVEKAPCATSSPVTQDDLQYHNLSKQQNESPQPLVETGKKSPESLVKLDATPLSSPRHVRIKNWGSGMTFQDTLHHKAKGILTCRSKSCLGSIMTPKSLTRGPRDKPTPPDELLPQAIEFVNQYYGSFKEAKIEEHLARVEAVTKEIETTGTYQLTGDELIFATKQAWRNAPRCIGRIQWSNLQVFDARSCSTAREMFEHICRHVRYSTNNGNIRSAITVFPQRSDGKHDFRVWNAQLIRYAGYQMPDGSIRGDPANVEFTQLCIDLGWKPKYGRFDVVPLVLQANGRDPELFEIPPDLVLEVAMEHPKYEWFRELELKWYALPAVANMLLEVGGLEFPGCPFNGWYMGTEIGVRDFCDVQRYNILEEVGRRMGLETHKLASLWKDQAVVEINIAVLHSFQKQNVTIMDHHSAAESFMKYMQNEYRSRGGCPADWIWLVPPMSGSITPVFHQEMLNYVLSPFYYYQVEAWKTHVWQDEKRRPKRREIPLKVLVKAVLFACMLMRKTMASRVRVTILFATETGKSEALAWDLGALFSCAFNPKVVCMDKYRLSCLEEERLLLVVTSTFGNGDCPGNGEKLKKSLFMLKELNNKFRYAVFGLGSSMYPRFCAFAHDIDQKLSHLGASQLTPMGEGDELSGQEDAFRSWAVQTFKAACETFDVRGKQHIQIPKLYTSNVTWDPHHYRLVQDSQPLDLSKALSSMHAKNVFTMRLKSRQNLQSPTSSRATILVELSCEDGQGLNYLPGEHLGVCPGNQPALVQGILERVVDGPTPHQTVRLEALDESGSYWVSDKRLPPCSLSQALTYFLDITTPPTQLLLQKLAQVATEEPERQRLEALCQPSEYSKWKFTNSPTFLEVLEEFPSLRVSAGFLLSQLPILKPRFYSISSSRDHTPTEIHLTVAVVTYHTRDGQGPLHHGVCSTWLNSLKPQDPVPCFVRNASGFHLPEDPSHPCILIGPGTGIAPFRSFWQQRLHDSQHKGVRGGRMTLVFGCRRPDEDHIYQEEMLEMAQKGVLHAVHTAYSRLPGKPKVYVQDILRQQLASEVLRVLHKEPGHLYVCGDVRMARDVAHTLKQLVAAKLKLNEEQVEDYFFQLKSQKRYHEDIFGAVFPYEAKKDRVAVQPSSLEMSAL

Target ORF information:

RefSeq Version	XM_011524859.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide synthase 2 (NOS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011524859.2

ORF Insert Sequence:

ATGGCCTGTC CTTGGAAATT TCTGTTCAAG ACCAAATTCC ACCAGTATGC AATGAATGGG 
GAAAAAGACA TCAACAACAA TGTGGAGAAA GCCCCCTGTG CCACCTCCAG TCCAGTGACA 
CAGGATGACC TTCAGTATCA CAACCTCAGC AAGCAGCAGA ATGAGTCCCC GCAGCCCCTC 
GTGGAGACGG GAAAGAAGTC TCCAGAATCT CTGGTCAAGC TGGATGCAAC CCCATTGTCC 
TCCCCACGGC ATGTGAGGAT CAAAAACTGG GGCAGCGGGA TGACTTTCCA AGACACACTT 
CACCATAAGG CCAAAGGGAT TTTAACTTGC AGGTCCAAAT CTTGCCTGGG GTCCATTATG 
ACTCCCAAAA GTTTGACCAG AGGACCCAGG GACAAGCCTA CCCCTCCAGA TGAGCTTCTA 
CCTCAAGCTA TCGAATTTGT CAACCAATAT TACGGCTCCT TCAAAGAGGC AAAAATAGAG 
GAACATCTGG CCAGGGTGGA AGCGGTAACA AAGGAGATAG AAACAACAGG AACCTACCAA 
CTGACGGGAG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAATGC CCCACGCTGC 
ATTGGGAGGA TCCAGTGGTC CAACCTGCAG GTCTTCGATG CCCGCAGCTG TTCCACTGCC 
CGGGAAATGT TTGAACACAT CTGCAGACAC GTGCGTTACT CCACCAACAA TGGCAACATC 
AGGTCGGCCA TCACCGTGTT CCCCCAGCGG AGTGATGGCA AGCACGACTT CCGGGTGTGG 
AATGCTCAGC TCATCCGCTA TGCTGGCTAC CAGATGCCAG ATGGCAGCAT CAGAGGGGAC 
CCTGCCAACG TGGAATTCAC TCAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC 
CGCTTCGATG TGGTCCCCCT GGTCCTGCAG GCCAATGGCC GTGACCCTGA GCTCTTCGAA 
ATCCCACCTG ACCTTGTGCT TGAGGTGGCC ATGGAACATC CCAAATACGA GTGGTTTCGG 
GAACTGGAGC TAAAGTGGTA CGCCCTGCCT GCAGTGGCCA ACATGCTGCT TGAGGTGGGC 
GGCCTGGAGT TCCCAGGGTG CCCCTTCAAT GGCTGGTACA TGGGCACAGA GATCGGAGTC 
CGGGACTTCT GTGACGTCCA GCGCTACAAC ATCCTGGAGG AAGTGGGCAG GAGAATGGGC 
CTGGAAACGC ACAAGCTGGC CTCGCTCTGG AAAGACCAGG CTGTCGTTGA GATCAACATT 
GCTGTGCTCC ATAGTTTCCA GAAGCAGAAT GTGACCATCA TGGACCACCA CTCGGCTGCA 
GAATCCTTCA TGAAGTACAT GCAGAATGAA TACCGGTCCC GTGGGGGCTG CCCGGCAGAC 
TGGATTTGGC TGGTCCCTCC CATGTCTGGG AGCATCACCC CCGTGTTTCA CCAGGAGATG 
CTGAACTACG TCCTGTCCCC TTTCTACTAC TATCAGGTAG AGGCCTGGAA AACCCATGTC 
TGGCAGGACG AGAAGCGGAG ACCCAAGAGA AGAGAGATTC CATTGAAAGT CTTGGTCAAA 
GCTGTGCTCT TTGCCTGTAT GCTGATGCGC AAGACAATGG CGTCCCGAGT CAGAGTCACC 
ATCCTCTTTG CGACAGAGAC AGGAAAATCA GAGGCGCTGG CCTGGGACCT GGGGGCCTTA 
TTCAGCTGTG CCTTCAACCC CAAGGTTGTC TGCATGGATA AGTACAGGCT GAGCTGCCTG 
GAGGAGGAAC GGCTGCTGTT GGTGGTGACC AGTACGTTTG GCAATGGAGA CTGCCCTGGC 
AATGGAGAGA AACTGAAGAA ATCGCTCTTC ATGCTGAAAG AGCTCAACAA CAAATTCAGG 
TACGCTGTGT TTGGCCTCGG CTCCAGCATG TACCCTCGGT TCTGCGCCTT TGCTCATGAC 
ATTGATCAGA AGCTGTCCCA CCTGGGGGCC TCTCAGCTCA CCCCGATGGG AGAAGGGGAT 
GAGCTCAGTG GGCAGGAGGA CGCCTTCCGC AGCTGGGCCG TGCAAACCTT CAAGGCAGCC 
TGTGAGACGT TTGATGTCCG AGGCAAACAG CACATTCAGA TCCCCAAGCT CTACACCTCC 
AATGTGACCT GGGACCCGCA CCACTACAGG CTCGTGCAGG ACTCACAGCC TTTGGACCTC 
AGCAAAGCCC TCAGCAGCAT GCATGCCAAG AACGTGTTCA CCATGAGGCT CAAATCTCGG 
CAGAATCTAC AAAGTCCGAC ATCCAGCCGT GCCACCATCC TGGTGGAACT CTCCTGTGAG 
GATGGCCAAG GCCTGAACTA CCTGCCGGGG GAGCACCTTG GGGTTTGCCC AGGCAACCAG 
CCGGCCCTGG TCCAAGGTAT CCTGGAGCGA GTGGTGGATG GCCCCACACC CCACCAGACA 
GTGCGCCTGG AGGCCCTGGA TGAGAGTGGC AGCTACTGGG TCAGTGACAA GAGGCTGCCC 
CCCTGCTCAC TCAGCCAGGC CCTCACCTAC TTCCTGGACA TCACCACACC CCCAACCCAG 
CTGCTGCTCC AAAAGCTGGC CCAGGTGGCC ACAGAAGAGC CTGAGAGACA GAGGCTGGAG 
GCCCTGTGCC AGCCCTCAGA GTACAGCAAG TGGAAGTTCA CCAACAGCCC CACATTCCTG 
GAGGTGCTAG AGGAGTTCCC GTCCCTGCGG GTGTCTGCTG GCTTCCTGCT TTCCCAGCTC 
CCCATTCTGA AGCCCAGGTT CTACTCCATC AGCTCCTCCC GGGATCACAC GCCCACAGAG 
ATCCACCTGA CTGTGGCCGT GGTCACCTAC CACACCCGAG ATGGCCAGGG TCCCCTGCAC 
CACGGCGTCT GCAGCACATG GCTCAACAGC CTGAAGCCCC AAGACCCAGT GCCCTGCTTT 
GTGCGGAATG CCAGCGGCTT CCACCTCCCC GAGGATCCCT CCCATCCTTG CATCCTCATC 
GGGCCTGGCA CAGGCATCGC GCCCTTCCGC AGTTTCTGGC AGCAACGGCT CCATGACTCC 
CAGCACAAGG GAGTGCGGGG AGGCCGCATG ACCTTGGTGT TTGGGTGCCG CCGCCCAGAT 
GAGGACCACA TCTACCAGGA GGAGATGCTG GAGATGGCCC AGAAGGGGGT GCTGCATGCG 
GTGCACACAG CCTATTCCCG CCTGCCTGGC AAGCCCAAGG TCTATGTTCA GGACATCCTG 
CGGCAGCAGC TGGCCAGCGA GGTGCTCCGT GTGCTCCACA AGGAGCCAGG CCACCTCTAT 
GTTTGCGGGG ATGTGCGCAT GGCCCGGGAC GTGGCCCACA CCCTGAAGCA GCTGGTGGCT 
GCCAAGCTGA AATTGAATGA GGAGCAGGTC GAGGACTATT TCTTTCAGCT CAAGAGCCAG 
AAGCGCTATC ACGAAGATAT CTTTGGTGCT GTATTTCCTT ACGAGGCGAA GAAGGACAGG 
GTGGCGGTGC AGCCCAGCAG CCTGGAGATG TCAGCGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS2 ( XM_011524860.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523162.1 Homo sapiens nitric oxide synthase 2 (NOS2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu59288
Clone ID Related Accession (Same CDS sequence)	XM_011524860.2
Accession Version	XM_011524860.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nitric oxide synthase, inducible isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524860.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGAGG GGCTGGGCGC GGCGTGCGAG GCCGCCGGGC CCCGGCTGTG CAGGCTGCTC 
TGCAGGATCC TCCGGCCGAA GCCTGACTGC TGCCCGCGCA GAGCCAGTCC AGTGACACAG 
GATGACCTTC AGTATCACAA CCTCAGCAAG CAGCAGAATG AGTCCCCGCA GCCCCTCGTG 
GAGACGGGAA AGAAGTCTCC AGAATCTCTG GTCAAGCTGG ATGCAACCCC ATTGTCCTCC 
CCACGGCATG TGAGGATCAA AAACTGGGGC AGCGGGATGA CTTTCCAAGA CACACTTCAC 
CATAAGGCCA AAGGGATTTT AACTTGCAGG TCCAAATCTT GCCTGGGGTC CATTATGACT 
CCCAAAAGTT TGACCAGAGG ACCCAGGGAC AAGCCTACCC CTCCAGATGA GCTTCTACCT 
CAAGCTATCG AATTTGTCAA CCAATATTAC GGCTCCTTCA AAGAGGCAAA AATAGAGGAA 
CATCTGGCCA GGGTGGAAGC GGTAACAAAG GAGATAGAAA CAACAGGAAC CTACCAACTG 
ACGGGAGATG AGCTCATCTT CGCCACCAAG CAGGCCTGGC GCAATGCCCC ACGCTGCATT 
GGGAGGATCC AGTGGTCCAA CCTGCAGGTC TTCGATGCCC GCAGCTGTTC CACTGCCCGG 
GAAATGTTTG AACACATCTG CAGACACGTG CGTTACTCCA CCAACAATGG CAACATCAGG 
TCGGCCATCA CCGTGTTCCC CCAGCGGAGT GATGGCAAGC ACGACTTCCG GGTGTGGAAT 
GCTCAGCTCA TCCGCTATGC TGGCTACCAG ATGCCAGATG GCAGCATCAG AGGGGACCCT 
GCCAACGTGG AATTCACTCA GCTGTGCATC GACCTGGGCT GGAAGCCCAA GTACGGCCGC 
TTCGATGTGG TCCCCCTGGT CCTGCAGGCC AATGGCCGTG ACCCTGAGCT CTTCGAAATC 
CCACCTGACC TTGTGCTTGA GGTGGCCATG GAACATCCCA AATACGAGTG GTTTCGGGAA 
CTGGAGCTAA AGTGGTACGC CCTGCCTGCA GTGGCCAACA TGCTGCTTGA GGTGGGCGGC 
CTGGAGTTCC CAGGGTGCCC CTTCAATGGC TGGTACATGG GCACAGAGAT CGGAGTCCGG 
GACTTCTGTG ACGTCCAGCG CTACAACATC CTGGAGGAAG TGGGCAGGAG AATGGGCCTG 
GAAACGCACA AGCTGGCCTC GCTCTGGAAA GACCAGGCTG TCGTTGAGAT CAACATTGCT 
GTGCTCCATA GTTTCCAGAA GCAGAATGTG ACCATCATGG ACCACCACTC GGCTGCAGAA 
TCCTTCATGA AGTACATGCA GAATGAATAC CGGTCCCGTG GGGGCTGCCC GGCAGACTGG 
ATTTGGCTGG TCCCTCCCAT GTCTGGGAGC ATCACCCCCG TGTTTCACCA GGAGATGCTG 
AACTACGTCC TGTCCCCTTT CTACTACTAT CAGGTAGAGG CCTGGAAAAC CCATGTCTGG 
CAGGACGAGA AGCGGAGACC CAAGAGAAGA GAGATTCCAT TGAAAGTCTT GGTCAAAGCT 
GTGCTCTTTG CCTGTATGCT GATGCGCAAG ACAATGGCGT CCCGAGTCAG AGTCACCATC 
CTCTTTGCGA CAGAGACAGG AAAATCAGAG GCGCTGGCCT GGGACCTGGG GGCCTTATTC 
AGCTGTGCCT TCAACCCCAA GGTTGTCTGC ATGGATAAGT ACAGGCTGAG CTGCCTGGAG 
GAGGAACGGC TGCTGTTGGT GGTGACCAGT ACGTTTGGCA ATGGAGACTG CCCTGGCAAT 
GGAGAGAAAC TGAAGAAATC GCTCTTCATG CTGAAAGAGC TCAACAACAA ATTCAGGTAC 
GCTGTGTTTG GCCTCGGCTC CAGCATGTAC CCTCGGTTCT GCGCCTTTGC TCATGACATT 
GATCAGAAGC TGTCCCACCT GGGGGCCTCT CAGCTCACCC CGATGGGAGA AGGGGATGAG 
CTCAGTGGGC AGGAGGACGC CTTCCGCAGC TGGGCCGTGC AAACCTTCAA GGCAGCCTGT 
GAGACGTTTG ATGTCCGAGG CAAACAGCAC ATTCAGATCC CCAAGCTCTA CACCTCCAAT 
GTGACCTGGG ACCCGCACCA CTACAGGCTC GTGCAGGACT CACAGCCTTT GGACCTCAGC 
AAAGCCCTCA GCAGCATGCA TGCCAAGAAC GTGTTCACCA TGAGGCTCAA ATCTCGGCAG 
AATCTACAAA GTCCGACATC CAGCCGTGCC ACCATCCTGG TGGAACTCTC CTGTGAGGAT 
GGCCAAGGCC TGAACTACCT GCCGGGGGAG CACCTTGGGG TTTGCCCAGG CAACCAGCCG 
GCCCTGGTCC AAGGTATCCT GGAGCGAGTG GTGGATGGCC CCACACCCCA CCAGACAGTG 
CGCCTGGAGG CCCTGGATGA GAGTGGCAGC TACTGGGTCA GTGACAAGAG GCTGCCCCCC 
TGCTCACTCA GCCAGGCCCT CACCTACTTC CTGGACATCA CCACACCCCC AACCCAGCTG 
CTGCTCCAAA AGCTGGCCCA GGTGGCCACA GAAGAGCCTG AGAGACAGAG GCTGGAGGCC 
CTGTGCCAGC CCTCAGAGTA CAGCAAGTGG AAGTTCACCA ACAGCCCCAC ATTCCTGGAG 
GTGCTAGAGG AGTTCCCGTC CCTGCGGGTG TCTGCTGGCT TCCTGCTTTC CCAGCTCCCC 
ATTCTGAAGC CCAGGTTCTA CTCCATCAGC TCCTCCCGGG ATCACACGCC CACAGAGATC 
CACCTGACTG TGGCCGTGGT CACCTACCAC ACCCGAGATG GCCAGGGTCC CCTGCACCAC 
GGCGTCTGCA GCACATGGCT CAACAGCCTG AAGCCCCAAG ACCCAGTGCC CTGCTTTGTG 
CGGAATGCCA GCGGCTTCCA CCTCCCCGAG GATCCCTCCC ATCCTTGCAT CCTCATCGGG 
CCTGGCACAG GCATCGCGCC CTTCCGCAGT TTCTGGCAGC AACGGCTCCA TGACTCCCAG 
CACAAGGGAG TGCGGGGAGG CCGCATGACC TTGGTGTTTG GGTGCCGCCG CCCAGATGAG 
GACCACATCT ACCAGGAGGA GATGCTGGAG ATGGCCCAGA AGGGGGTGCT GCATGCGGTG 
CACACAGCCT ATTCCCGCCT GCCTGGCAAG CCCAAGGTCT ATGTTCAGGA CATCCTGCGG 
CAGCAGCTGG CCAGCGAGGT GCTCCGTGTG CTCCACAAGG AGCCAGGCCA CCTCTATGTT 
TGCGGGGATG TGCGCATGGC CCGGGACGTG GCCCACACCC TGAAGCAGCT GGTGGCTGCC 
AAGCTGAAAT TGAATGAGGA GCAGGTCGAG GACTATTTCT TTCAGCTCAA GAGCCAGAAG 
CGCTATCACG AAGATATCTT TGGTGCTGTA TTTCCTTACG AGGCGAAGAA GGACAGGGTG 
GCGGTGCAGC CCAGCAGCCT GGAGATGTCA GCGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523162.1
CDS	87..3545
Translation	MREGLGAACEAAGPRLCRLLCRILRPKPDCCPRRASPVTQDDLQYHNLSKQQNESPQPLVETGKKSPESLVKLDATPLSSPRHVRIKNWGSGMTFQDTLHHKAKGILTCRSKSCLGSIMTPKSLTRGPRDKPTPPDELLPQAIEFVNQYYGSFKEAKIEEHLARVEAVTKEIETTGTYQLTGDELIFATKQAWRNAPRCIGRIQWSNLQVFDARSCSTAREMFEHICRHVRYSTNNGNIRSAITVFPQRSDGKHDFRVWNAQLIRYAGYQMPDGSIRGDPANVEFTQLCIDLGWKPKYGRFDVVPLVLQANGRDPELFEIPPDLVLEVAMEHPKYEWFRELELKWYALPAVANMLLEVGGLEFPGCPFNGWYMGTEIGVRDFCDVQRYNILEEVGRRMGLETHKLASLWKDQAVVEINIAVLHSFQKQNVTIMDHHSAAESFMKYMQNEYRSRGGCPADWIWLVPPMSGSITPVFHQEMLNYVLSPFYYYQVEAWKTHVWQDEKRRPKRREIPLKVLVKAVLFACMLMRKTMASRVRVTILFATETGKSEALAWDLGALFSCAFNPKVVCMDKYRLSCLEEERLLLVVTSTFGNGDCPGNGEKLKKSLFMLKELNNKFRYAVFGLGSSMYPRFCAFAHDIDQKLSHLGASQLTPMGEGDELSGQEDAFRSWAVQTFKAACETFDVRGKQHIQIPKLYTSNVTWDPHHYRLVQDSQPLDLSKALSSMHAKNVFTMRLKSRQNLQSPTSSRATILVELSCEDGQGLNYLPGEHLGVCPGNQPALVQGILERVVDGPTPHQTVRLEALDESGSYWVSDKRLPPCSLSQALTYFLDITTPPTQLLLQKLAQVATEEPERQRLEALCQPSEYSKWKFTNSPTFLEVLEEFPSLRVSAGFLLSQLPILKPRFYSISSSRDHTPTEIHLTVAVVTYHTRDGQGPLHHGVCSTWLNSLKPQDPVPCFVRNASGFHLPEDPSHPCILIGPGTGIAPFRSFWQQRLHDSQHKGVRGGRMTLVFGCRRPDEDHIYQEEMLEMAQKGVLHAVHTAYSRLPGKPKVYVQDILRQQLASEVLRVLHKEPGHLYVCGDVRMARDVAHTLKQLVAAKLKLNEEQVEDYFFQLKSQKRYHEDIFGAVFPYEAKKDRVAVQPSSLEMSAL

Target ORF information:

RefSeq Version	XM_011524860.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide synthase 2 (NOS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011524860.2

ORF Insert Sequence:

ATGCGCGAGG GGCTGGGCGC GGCGTGCGAG GCCGCCGGGC CCCGGCTGTG CAGGCTGCTC 
TGCAGGATCC TCCGGCCGAA GCCTGACTGC TGCCCGCGCA GAGCCAGTCC AGTGACACAG 
GATGACCTTC AGTATCACAA CCTCAGCAAG CAGCAGAATG AGTCCCCGCA GCCCCTCGTG 
GAGACGGGAA AGAAGTCTCC AGAATCTCTG GTCAAGCTGG ATGCAACCCC ATTGTCCTCC 
CCACGGCATG TGAGGATCAA AAACTGGGGC AGCGGGATGA CTTTCCAAGA CACACTTCAC 
CATAAGGCCA AAGGGATTTT AACTTGCAGG TCCAAATCTT GCCTGGGGTC CATTATGACT 
CCCAAAAGTT TGACCAGAGG ACCCAGGGAC AAGCCTACCC CTCCAGATGA GCTTCTACCT 
CAAGCTATCG AATTTGTCAA CCAATATTAC GGCTCCTTCA AAGAGGCAAA AATAGAGGAA 
CATCTGGCCA GGGTGGAAGC GGTAACAAAG GAGATAGAAA CAACAGGAAC CTACCAACTG 
ACGGGAGATG AGCTCATCTT CGCCACCAAG CAGGCCTGGC GCAATGCCCC ACGCTGCATT 
GGGAGGATCC AGTGGTCCAA CCTGCAGGTC TTCGATGCCC GCAGCTGTTC CACTGCCCGG 
GAAATGTTTG AACACATCTG CAGACACGTG CGTTACTCCA CCAACAATGG CAACATCAGG 
TCGGCCATCA CCGTGTTCCC CCAGCGGAGT GATGGCAAGC ACGACTTCCG GGTGTGGAAT 
GCTCAGCTCA TCCGCTATGC TGGCTACCAG ATGCCAGATG GCAGCATCAG AGGGGACCCT 
GCCAACGTGG AATTCACTCA GCTGTGCATC GACCTGGGCT GGAAGCCCAA GTACGGCCGC 
TTCGATGTGG TCCCCCTGGT CCTGCAGGCC AATGGCCGTG ACCCTGAGCT CTTCGAAATC 
CCACCTGACC TTGTGCTTGA GGTGGCCATG GAACATCCCA AATACGAGTG GTTTCGGGAA 
CTGGAGCTAA AGTGGTACGC CCTGCCTGCA GTGGCCAACA TGCTGCTTGA GGTGGGCGGC 
CTGGAGTTCC CAGGGTGCCC CTTCAATGGC TGGTACATGG GCACAGAGAT CGGAGTCCGG 
GACTTCTGTG ACGTCCAGCG CTACAACATC CTGGAGGAAG TGGGCAGGAG AATGGGCCTG 
GAAACGCACA AGCTGGCCTC GCTCTGGAAA GACCAGGCTG TCGTTGAGAT CAACATTGCT 
GTGCTCCATA GTTTCCAGAA GCAGAATGTG ACCATCATGG ACCACCACTC GGCTGCAGAA 
TCCTTCATGA AGTACATGCA GAATGAATAC CGGTCCCGTG GGGGCTGCCC GGCAGACTGG 
ATTTGGCTGG TCCCTCCCAT GTCTGGGAGC ATCACCCCCG TGTTTCACCA GGAGATGCTG 
AACTACGTCC TGTCCCCTTT CTACTACTAT CAGGTAGAGG CCTGGAAAAC CCATGTCTGG 
CAGGACGAGA AGCGGAGACC CAAGAGAAGA GAGATTCCAT TGAAAGTCTT GGTCAAAGCT 
GTGCTCTTTG CCTGTATGCT GATGCGCAAG ACAATGGCGT CCCGAGTCAG AGTCACCATC 
CTCTTTGCGA CAGAGACAGG AAAATCAGAG GCGCTGGCCT GGGACCTGGG GGCCTTATTC 
AGCTGTGCCT TCAACCCCAA GGTTGTCTGC ATGGATAAGT ACAGGCTGAG CTGCCTGGAG 
GAGGAACGGC TGCTGTTGGT GGTGACCAGT ACGTTTGGCA ATGGAGACTG CCCTGGCAAT 
GGAGAGAAAC TGAAGAAATC GCTCTTCATG CTGAAAGAGC TCAACAACAA ATTCAGGTAC 
GCTGTGTTTG GCCTCGGCTC CAGCATGTAC CCTCGGTTCT GCGCCTTTGC TCATGACATT 
GATCAGAAGC TGTCCCACCT GGGGGCCTCT CAGCTCACCC CGATGGGAGA AGGGGATGAG 
CTCAGTGGGC AGGAGGACGC CTTCCGCAGC TGGGCCGTGC AAACCTTCAA GGCAGCCTGT 
GAGACGTTTG ATGTCCGAGG CAAACAGCAC ATTCAGATCC CCAAGCTCTA CACCTCCAAT 
GTGACCTGGG ACCCGCACCA CTACAGGCTC GTGCAGGACT CACAGCCTTT GGACCTCAGC 
AAAGCCCTCA GCAGCATGCA TGCCAAGAAC GTGTTCACCA TGAGGCTCAA ATCTCGGCAG 
AATCTACAAA GTCCGACATC CAGCCGTGCC ACCATCCTGG TGGAACTCTC CTGTGAGGAT 
GGCCAAGGCC TGAACTACCT GCCGGGGGAG CACCTTGGGG TTTGCCCAGG CAACCAGCCG 
GCCCTGGTCC AAGGTATCCT GGAGCGAGTG GTGGATGGCC CCACACCCCA CCAGACAGTG 
CGCCTGGAGG CCCTGGATGA GAGTGGCAGC TACTGGGTCA GTGACAAGAG GCTGCCCCCC 
TGCTCACTCA GCCAGGCCCT CACCTACTTC CTGGACATCA CCACACCCCC AACCCAGCTG 
CTGCTCCAAA AGCTGGCCCA GGTGGCCACA GAAGAGCCTG AGAGACAGAG GCTGGAGGCC 
CTGTGCCAGC CCTCAGAGTA CAGCAAGTGG AAGTTCACCA ACAGCCCCAC ATTCCTGGAG 
GTGCTAGAGG AGTTCCCGTC CCTGCGGGTG TCTGCTGGCT TCCTGCTTTC CCAGCTCCCC 
ATTCTGAAGC CCAGGTTCTA CTCCATCAGC TCCTCCCGGG ATCACACGCC CACAGAGATC 
CACCTGACTG TGGCCGTGGT CACCTACCAC ACCCGAGATG GCCAGGGTCC CCTGCACCAC 
GGCGTCTGCA GCACATGGCT CAACAGCCTG AAGCCCCAAG ACCCAGTGCC CTGCTTTGTG 
CGGAATGCCA GCGGCTTCCA CCTCCCCGAG GATCCCTCCC ATCCTTGCAT CCTCATCGGG 
CCTGGCACAG GCATCGCGCC CTTCCGCAGT TTCTGGCAGC AACGGCTCCA TGACTCCCAG 
CACAAGGGAG TGCGGGGAGG CCGCATGACC TTGGTGTTTG GGTGCCGCCG CCCAGATGAG 
GACCACATCT ACCAGGAGGA GATGCTGGAG ATGGCCCAGA AGGGGGTGCT GCATGCGGTG 
CACACAGCCT ATTCCCGCCT GCCTGGCAAG CCCAAGGTCT ATGTTCAGGA CATCCTGCGG 
CAGCAGCTGG CCAGCGAGGT GCTCCGTGTG CTCCACAAGG AGCCAGGCCA CCTCTATGTT 
TGCGGGGATG TGCGCATGGC CCGGGACGTG GCCCACACCC TGAAGCAGCT GGTGGCTGCC 
AAGCTGAAAT TGAATGAGGA GCAGGTCGAG GACTATTTCT TTCAGCTCAA GAGCCAGAAG 
CGCTATCACG AAGATATCTT TGGTGCTGTA TTTCCTTACG AGGCGAAGAA GGACAGGGTG 
GCGGTGCAGC CCAGCAGCCT GGAGATGTCA GCGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS2 ( XM_011524861.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523163.1 Homo sapiens nitric oxide synthase 2 (NOS2), transcript variant X3, mRNA.

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CloneID	OHu59289
Clone ID Related Accession (Same CDS sequence)	XM_011524861.1
Accession Version	XM_011524861.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nitric oxide synthase, inducible isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGTC CTTGGAAATT TCTGTTCAAG ACCAAATTCC ACCAGTATGC AATGAATGGG 
GAAAAAGACA TCAACAACAA TGTGGAGAAA GCCCCCTGTG CCACCTCCAG TCCAGTGACA 
CAGGATGACC TTCAGTATCA CAACCTCAGC AAGCAGCAGA ATGAGTCCCC GCAGCCCCTC 
GTGGAGACGG GAAAGAAGTC TCCAGAATCT CTGGTCAAGC TGGATGCAAC CCCATTGTCC 
TCCCCACGGC ATGTGAGGAT CAAAAACTGG GGCAGCGGGA TGACTTTCCA AGACACACTT 
CACCATAAGG CCAAAGGGAT TTTAACTTGC AGGTCCAAAT CTTGCCTGGG GTCCATTATG 
ACTCCCAAAA GTTTGACCAG AGGACCCAGG GACAAGCCTA CCCCTCCAGA TGAGCTTCTA 
CCTCAAGCTA TCGAATTTGT CAACCAATAT TACGGCTCCT TCAAAGAGGC AAAAATAGAG 
GAACATCTGG CCAGGGTGGA AGCGGTAACA AAGGAGATAG AAACAACAGG AACCTACCAA 
CTGACGGGAG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAATGC CCCACGCTGC 
ATTGGGAGGA TCCAGTGGTC CAACCTGCAG GTCTTCGATG CCCGCAGCTG TTCCACTGCC 
CGGGAAATGT TTGAACACAT CTGCAGACAC GTGCGTTACT CCACCAACAA TGGCAACATC 
AGGTCGGCCA TCACCGTGTT CCCCCAGCGG AGTGATGGCA AGCACGACTT CCGGGTGTGG 
AATGCTCAGC TCATCCGCTA TGCTGGCTAC CAGATGCCAG ATGGCAGCAT CAGAGGGGAC 
CCTGCCAACG TGGAATTCAC TCAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC 
CGCTTCGATG TGGTCCCCCT GGTCCTGCAG GCCAATGGCC GTGACCCTGA GCTCTTCGAA 
ATCCCACCTG ACCTTGTGCT TGAGGTGGCC ATGGAACATC CCAAATACGA GTGGTTTCGG 
GAACTGGAGC TAAAGTGGTA CGCCCTGCCT GCAGTGGCCA ACATGCTGCT TGAGGTGGGC 
GGCCTGGAGT TCCCAGGGTG CCCCTTCAAT GGCTGGTACA TGGGCACAGA GATCGGAGTC 
CGGGACTTCT GTGACGTCCA GCGCTACAAC ATCCTGGAGG AAGTGGGCAG GAGAATGGGC 
CTGGAAACGC ACAAGCTGGC CTCGCTCTGG AAAGACCAGG CTGTCGTTGA GATCAACATT 
GCTGTGCTCC ATAGTTTCCA GAAGCAGAAT GTGACCATCA TGGACCACCA CTCGGCTGCA 
GAATCCTTCA TGAAGTACAT GCAGAATGAA TACCGGTCCC GTGGGGGCTG CCCGGCAGAC 
TGGATTTGGC TGGTCCCTCC CATGTCTGGG AGCATCACCC CCGTGTTTCA CCAGGAGATG 
CTGAACTACG TCCTGTCCCC TTTCTACTAC TATCAGGTAG AGGCCTGGAA AACCCATGTC 
TGGCAGGACG AGAAGCGGAG ACCCAAGAGA AGAGAGATTC CATTGAAAGT CTTGGTCAAA 
GCTGTGCTCT TTGCCTGTAT GCTGATGCGC AAGACAATGG CGTCCCGAGT CAGAGTCACC 
ATCCTCTTTG CGACAGAGAC AGGAAAATCA GAGGCGCTGG CCTGGGACCT GGGGGCCTTA 
TTCAGCTGTG CCTTCAACCC CAAGGTTGTC TGCATGGATA AGTACAGGCT GAGCTGCCTG 
GAGGAGGAAC GGCTGCTGTT GGTGGTGACC AGTACGTTTG GCAATGGAGA CTGCCCTGGC 
AATGGAGAGA AACTGAAGAA ATCGCTCTTC ATGCTGAAAG AGCTCAACAA CAAATTCAGG 
TACGCTGTGT TTGGCCTCGG CTCCAGCATG TACCCTCGGT TCTGCGCCTT TGCTCATGAC 
ATTGATCAGA AGCTGTCCCA CCTGGGGGCC TCTCAGCTCA CCCCGATGGG AGAAGGGGAT 
GAGCTCAGTG GGCAGGAGGA CGCCTTCCGC AGCTGGGCCG TGCAAACCTT CAAGGCAGCC 
TGTGAGACGT TTGATGTCCG AGGCAAACAG CACATTCAGA TCCCCAAGCT CTACACCTCC 
AATGTGACCT GGGACCCGCA CCACTACAGG CTCGTGCAGG ACTCACAGCC TTTGGACCTC 
AGCAAAGCCC TCAGCAGCAT GCATGCCAAG AACGTGTTCA CCATGAGGCT CAAATCTCGG 
CAGAATCTAC AAAGTCCGAC ATCCAGCCGT GCCACCATCC TGGTGGAACT CTCCTGTGAG 
GATGGCCAAG GCCTGAACTA CCTGCCGGGG GAGCACCTTG GGGTTTGCCC AGGCAACCAG 
CCGGCCCTGG TCCAAGGCAG CTACTGGGTC AGTGACAAGA GGCTGCCCCC CTGCTCACTC 
AGCCAGGCCC TCACCTACTT CCTGGACATC ACCACACCCC CAACCCAGCT GCTGCTCCAA 
AAGCTGGCCC AGGTGGCCAC AGAAGAGCCT GAGAGACAGA GGCTGGAGGC CCTGTGCCAG 
CCCTCAGAGT ACAGCAAGTG GAAGTTCACC AACAGCCCCA CATTCCTGGA GGTGCTAGAG 
GAGTTCCCGT CCCTGCGGGT GTCTGCTGGC TTCCTGCTTT CCCAGCTCCC CATTCTGAAG 
CCCAGGTTCT ACTCCATCAG CTCCTCCCGG GATCACACGC CCACAGAGAT CCACCTGACT 
GTGGCCGTGG TCACCTACCA CACCCGAGAT GGCCAGGGTC CCCTGCACCA CGGCGTCTGC 
AGCACATGGC TCAACAGCCT GAAGCCCCAA GACCCAGTGC CCTGCTTTGT GCGGAATGCC 
AGCGGCTTCC ACCTCCCCGA GGATCCCTCC CATCCTTGCA TCCTCATCGG GCCTGGCACA 
GGCATCGCGC CCTTCCGCAG TTTCTGGCAG CAACGGCTCC ATGACTCCCA GCACAAGGGA 
GTGCGGGGAG GCCGCATGAC CTTGGTGTTT GGGTGCCGCC GCCCAGATGA GGACCACATC 
TACCAGGAGG AGATGCTGGA GATGGCCCAG AAGGGGGTGC TGCATGCGGT GCACACAGCC 
TATTCCCGCC TGCCTGGCAA GCCCAAGGTC TATGTTCAGG ACATCCTGCG GCAGCAGCTG 
GCCAGCGAGG TGCTCCGTGT GCTCCACAAG GAGCCAGGCC ACCTCTATGT TTGCGGGGAT 
GTGCGCATGG CCCGGGACGT GGCCCACACC CTGAAGCAGC TGGTGGCTGC CAAGCTGAAA 
TTGAATGAGG AGCAGGTCGA GGACTATTTC TTTCAGCTCA AGAGCCAGAA GCGCTATCAC 
GAAGATATCT TTGGTGCTGT ATTTCCTTAC GAGGCGAAGA AGGACAGGGT GGCGGTGCAG 
CCCAGCAGCC TGGAGATGTC AGCGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523163.1
CDS	263..3652
Translation	MACPWKFLFKTKFHQYAMNGEKDINNNVEKAPCATSSPVTQDDLQYHNLSKQQNESPQPLVETGKKSPESLVKLDATPLSSPRHVRIKNWGSGMTFQDTLHHKAKGILTCRSKSCLGSIMTPKSLTRGPRDKPTPPDELLPQAIEFVNQYYGSFKEAKIEEHLARVEAVTKEIETTGTYQLTGDELIFATKQAWRNAPRCIGRIQWSNLQVFDARSCSTAREMFEHICRHVRYSTNNGNIRSAITVFPQRSDGKHDFRVWNAQLIRYAGYQMPDGSIRGDPANVEFTQLCIDLGWKPKYGRFDVVPLVLQANGRDPELFEIPPDLVLEVAMEHPKYEWFRELELKWYALPAVANMLLEVGGLEFPGCPFNGWYMGTEIGVRDFCDVQRYNILEEVGRRMGLETHKLASLWKDQAVVEINIAVLHSFQKQNVTIMDHHSAAESFMKYMQNEYRSRGGCPADWIWLVPPMSGSITPVFHQEMLNYVLSPFYYYQVEAWKTHVWQDEKRRPKRREIPLKVLVKAVLFACMLMRKTMASRVRVTILFATETGKSEALAWDLGALFSCAFNPKVVCMDKYRLSCLEEERLLLVVTSTFGNGDCPGNGEKLKKSLFMLKELNNKFRYAVFGLGSSMYPRFCAFAHDIDQKLSHLGASQLTPMGEGDELSGQEDAFRSWAVQTFKAACETFDVRGKQHIQIPKLYTSNVTWDPHHYRLVQDSQPLDLSKALSSMHAKNVFTMRLKSRQNLQSPTSSRATILVELSCEDGQGLNYLPGEHLGVCPGNQPALVQGSYWVSDKRLPPCSLSQALTYFLDITTPPTQLLLQKLAQVATEEPERQRLEALCQPSEYSKWKFTNSPTFLEVLEEFPSLRVSAGFLLSQLPILKPRFYSISSSRDHTPTEIHLTVAVVTYHTRDGQGPLHHGVCSTWLNSLKPQDPVPCFVRNASGFHLPEDPSHPCILIGPGTGIAPFRSFWQQRLHDSQHKGVRGGRMTLVFGCRRPDEDHIYQEEMLEMAQKGVLHAVHTAYSRLPGKPKVYVQDILRQQLASEVLRVLHKEPGHLYVCGDVRMARDVAHTLKQLVAAKLKLNEEQVEDYFFQLKSQKRYHEDIFGAVFPYEAKKDRVAVQPSSLEMSAL

Target ORF information:

RefSeq Version	XM_011524861.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nitric oxide synthase 2 (NOS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011524861.1

ORF Insert Sequence:

ATGGCCTGTC CTTGGAAATT TCTGTTCAAG ACCAAATTCC ACCAGTATGC AATGAATGGG 
GAAAAAGACA TCAACAACAA TGTGGAGAAA GCCCCCTGTG CCACCTCCAG TCCAGTGACA 
CAGGATGACC TTCAGTATCA CAACCTCAGC AAGCAGCAGA ATGAGTCCCC GCAGCCCCTC 
GTGGAGACGG GAAAGAAGTC TCCAGAATCT CTGGTCAAGC TGGATGCAAC CCCATTGTCC 
TCCCCACGGC ATGTGAGGAT CAAAAACTGG GGCAGCGGGA TGACTTTCCA AGACACACTT 
CACCATAAGG CCAAAGGGAT TTTAACTTGC AGGTCCAAAT CTTGCCTGGG GTCCATTATG 
ACTCCCAAAA GTTTGACCAG AGGACCCAGG GACAAGCCTA CCCCTCCAGA TGAGCTTCTA 
CCTCAAGCTA TCGAATTTGT CAACCAATAT TACGGCTCCT TCAAAGAGGC AAAAATAGAG 
GAACATCTGG CCAGGGTGGA AGCGGTAACA AAGGAGATAG AAACAACAGG AACCTACCAA 
CTGACGGGAG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAATGC CCCACGCTGC 
ATTGGGAGGA TCCAGTGGTC CAACCTGCAG GTCTTCGATG CCCGCAGCTG TTCCACTGCC 
CGGGAAATGT TTGAACACAT CTGCAGACAC GTGCGTTACT CCACCAACAA TGGCAACATC 
AGGTCGGCCA TCACCGTGTT CCCCCAGCGG AGTGATGGCA AGCACGACTT CCGGGTGTGG 
AATGCTCAGC TCATCCGCTA TGCTGGCTAC CAGATGCCAG ATGGCAGCAT CAGAGGGGAC 
CCTGCCAACG TGGAATTCAC TCAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC 
CGCTTCGATG TGGTCCCCCT GGTCCTGCAG GCCAATGGCC GTGACCCTGA GCTCTTCGAA 
ATCCCACCTG ACCTTGTGCT TGAGGTGGCC ATGGAACATC CCAAATACGA GTGGTTTCGG 
GAACTGGAGC TAAAGTGGTA CGCCCTGCCT GCAGTGGCCA ACATGCTGCT TGAGGTGGGC 
GGCCTGGAGT TCCCAGGGTG CCCCTTCAAT GGCTGGTACA TGGGCACAGA GATCGGAGTC 
CGGGACTTCT GTGACGTCCA GCGCTACAAC ATCCTGGAGG AAGTGGGCAG GAGAATGGGC 
CTGGAAACGC ACAAGCTGGC CTCGCTCTGG AAAGACCAGG CTGTCGTTGA GATCAACATT 
GCTGTGCTCC ATAGTTTCCA GAAGCAGAAT GTGACCATCA TGGACCACCA CTCGGCTGCA 
GAATCCTTCA TGAAGTACAT GCAGAATGAA TACCGGTCCC GTGGGGGCTG CCCGGCAGAC 
TGGATTTGGC TGGTCCCTCC CATGTCTGGG AGCATCACCC CCGTGTTTCA CCAGGAGATG 
CTGAACTACG TCCTGTCCCC TTTCTACTAC TATCAGGTAG AGGCCTGGAA AACCCATGTC 
TGGCAGGACG AGAAGCGGAG ACCCAAGAGA AGAGAGATTC CATTGAAAGT CTTGGTCAAA 
GCTGTGCTCT TTGCCTGTAT GCTGATGCGC AAGACAATGG CGTCCCGAGT CAGAGTCACC 
ATCCTCTTTG CGACAGAGAC AGGAAAATCA GAGGCGCTGG CCTGGGACCT GGGGGCCTTA 
TTCAGCTGTG CCTTCAACCC CAAGGTTGTC TGCATGGATA AGTACAGGCT GAGCTGCCTG 
GAGGAGGAAC GGCTGCTGTT GGTGGTGACC AGTACGTTTG GCAATGGAGA CTGCCCTGGC 
AATGGAGAGA AACTGAAGAA ATCGCTCTTC ATGCTGAAAG AGCTCAACAA CAAATTCAGG 
TACGCTGTGT TTGGCCTCGG CTCCAGCATG TACCCTCGGT TCTGCGCCTT TGCTCATGAC 
ATTGATCAGA AGCTGTCCCA CCTGGGGGCC TCTCAGCTCA CCCCGATGGG AGAAGGGGAT 
GAGCTCAGTG GGCAGGAGGA CGCCTTCCGC AGCTGGGCCG TGCAAACCTT CAAGGCAGCC 
TGTGAGACGT TTGATGTCCG AGGCAAACAG CACATTCAGA TCCCCAAGCT CTACACCTCC 
AATGTGACCT GGGACCCGCA CCACTACAGG CTCGTGCAGG ACTCACAGCC TTTGGACCTC 
AGCAAAGCCC TCAGCAGCAT GCATGCCAAG AACGTGTTCA CCATGAGGCT CAAATCTCGG 
CAGAATCTAC AAAGTCCGAC ATCCAGCCGT GCCACCATCC TGGTGGAACT CTCCTGTGAG 
GATGGCCAAG GCCTGAACTA CCTGCCGGGG GAGCACCTTG GGGTTTGCCC AGGCAACCAG 
CCGGCCCTGG TCCAAGGCAG CTACTGGGTC AGTGACAAGA GGCTGCCCCC CTGCTCACTC 
AGCCAGGCCC TCACCTACTT CCTGGACATC ACCACACCCC CAACCCAGCT GCTGCTCCAA 
AAGCTGGCCC AGGTGGCCAC AGAAGAGCCT GAGAGACAGA GGCTGGAGGC CCTGTGCCAG 
CCCTCAGAGT ACAGCAAGTG GAAGTTCACC AACAGCCCCA CATTCCTGGA GGTGCTAGAG 
GAGTTCCCGT CCCTGCGGGT GTCTGCTGGC TTCCTGCTTT CCCAGCTCCC CATTCTGAAG 
CCCAGGTTCT ACTCCATCAG CTCCTCCCGG GATCACACGC CCACAGAGAT CCACCTGACT 
GTGGCCGTGG TCACCTACCA CACCCGAGAT GGCCAGGGTC CCCTGCACCA CGGCGTCTGC 
AGCACATGGC TCAACAGCCT GAAGCCCCAA GACCCAGTGC CCTGCTTTGT GCGGAATGCC 
AGCGGCTTCC ACCTCCCCGA GGATCCCTCC CATCCTTGCA TCCTCATCGG GCCTGGCACA 
GGCATCGCGC CCTTCCGCAG TTTCTGGCAG CAACGGCTCC ATGACTCCCA GCACAAGGGA 
GTGCGGGGAG GCCGCATGAC CTTGGTGTTT GGGTGCCGCC GCCCAGATGA GGACCACATC 
TACCAGGAGG AGATGCTGGA GATGGCCCAG AAGGGGGTGC TGCATGCGGT GCACACAGCC 
TATTCCCGCC TGCCTGGCAA GCCCAAGGTC TATGTTCAGG ACATCCTGCG GCAGCAGCTG 
GCCAGCGAGG TGCTCCGTGT GCTCCACAAG GAGCCAGGCC ACCTCTATGT TTGCGGGGAT 
GTGCGCATGG CCCGGGACGT GGCCCACACC CTGAAGCAGC TGGTGGCTGC CAAGCTGAAA 
TTGAATGAGG AGCAGGTCGA GGACTATTTC TTTCAGCTCA AGAGCCAGAA GCGCTATCAC 
GAAGATATCT TTGGTGCTGT ATTTCCTTAC GAGGCGAAGA AGGACAGGGT GGCGGTGCAG 
CCCAGCAGCC TGGAGATGTC AGCGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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NOS2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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