bicra cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	bicra
Entrez Gene ID	386775
Full Name	BRD4 interacting chromatin remodeling complex associated protein
Synonyms	gltscr1,sb:cb888,si:dkey-224e22.6,wu:fc28g11
General protein information	Preferred Names BRD4 interacting chromatin remodeling complex associated protein Names glioma tumor suppressor candidate region gene 1 protein glioma tumor suppressor candidate region gene 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005159252.3	XP_005159309.1	glioma tumor suppressor candidate region gene 1 protein isoform X1
XM_001922789.6	XP_001922824.3	glioma tumor suppressor candidate region gene 1 protein isoform X3
XM_005159254.3	XP_005159311.1	glioma tumor suppressor candidate region gene 1 protein isoform X4
XM_001922789.7	XP_001922824.3	glioma tumor suppressor candidate region gene 1 protein isoform X3
XM_005159253.3	XP_005159310.1	glioma tumor suppressor candidate region gene 1 protein isoform X2
XM_005159252.4	XP_005159309.1	glioma tumor suppressor candidate region gene 1 protein isoform X1
XM_005159253.4	XP_005159310.1	glioma tumor suppressor candidate region gene 1 protein isoform X2
XM_005159254.4	XP_005159311.1	glioma tumor suppressor candidate region gene 1 protein isoform X4

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GLTSCR1	XP_003587347.2
Danio rerio (zebrafish)	gltscr1	XP_005159310.1

Gene Ontology

The following bicra gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bicra cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa23558	XM_005159252.4 Latest version!	Danio rerio BRD4 interacting chromatin remodeling complex associated protein (bicra), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa23559	XM_005159253.4 Latest version!	Danio rerio BRD4 interacting chromatin remodeling complex associated protein (bicra), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa23560	XM_001922789.7 Latest version!	Danio rerio BRD4 interacting chromatin remodeling complex associated protein (bicra), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa23561	XM_005159254.4 Latest version!	Danio rerio BRD4 interacting chromatin remodeling complex associated protein (bicra), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa23560	XM_001922789.6	Danio rerio glioma tumor suppressor candidate region gene 1 (gltscr1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa23558	XM_005159252.3	Danio rerio glioma tumor suppressor candidate region gene 1 (gltscr1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa23559	XM_005159253.3	Danio rerio glioma tumor suppressor candidate region gene 1 (gltscr1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa23561	XM_005159254.3	Danio rerio glioma tumor suppressor candidate region gene 1 (gltscr1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bicra ( XM_005159252.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159309.1 Danio rerio glioma tumor suppressor candidate region gene 1 (gltscr1), transcript variant X1, mRNA.

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CloneID	ODa23558
Clone ID Related Accession (Same CDS sequence)	XM_005159252.3 , XM_005159252.4
Accession Version	XM_005159252.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	glioma tumor suppressor candidate region gene 1 protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159252.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATG AAGATGGCAG GTGCTTGCTA GATGTAATTT GTGACCCACA AGCACTCAAC 
GATTTTCTTC ATGGATCAGA AACACAACTG GACACTGATG ACCTATTGGA TGGCTCAGGT 
GACCCGTCCA GCTCGTTCTT CTCCAGCACT GGGGGCCATG TTCCTGAGGT TCCGGCACAG 
GTTCAGCTGT CGACAAGTGA GCCGGGCCTT CCAAGAGTCA GCGTTGATTT GGACTTCTTG 
GAGGACGATG ACATATTGGG GGGATCGCCA GGAGGAGACA ATGGAAGTAA TGGTGTTGGG 
ACAAATCATG AGCCCTGTGA CATTTTGCAG CAGAGCTTGG CGGAGGCTAA CATCACAGAG 
CAGAGCCTTC AAGAGGCAGA TGCTGAGCTT GACCTCAGTT CCTTTGGGCT AACTGGCCTT 
ACGCAGGTGG TTCAACCCCT GCCTGATGCC GGCCTGTCTG GGGTGGGCAT TGGAGGTGCA 
ACTCAGATTT TTCCAAACCA AGGCACCCCT ACCGCACCCT CAAATCCCAC TCCAGACATG 
CTTGGTTCTG TCCTGGCACA TCCAGGTCTG CAGCTTCAAC AACAGGTCAT GAACAAGGCT 
ATCAGCGTCC AGCCTTTTGT ACAACAAGTC GGACTTGGAA ATGTAACACT TCAGCCAATT 
TCGAGCCTTC AGGCCCTACC GAACGGCAGC CAGTCTGGAC CGTTGGGCAT TGGACAAATT 
CAAGTGGTGG GTCAGCCCAC TGTAATGACT ATAAATCCAT CTGGGCAGCA AATCTTGACG 
AAAACCATGG GTGGCTACCA ACTGCATCAA CCTGGGCCGG AGGCAGCAAG CGCTGGAACG 
CAGGCTGGGC TTGGAAGCTC TGTTTTGAGT TCAGGAGGTG GACTTGTAAT TCAAGGGGGC 
AAGGCCACTC TCGGGTCCCC TGCCTTAAAT GGGCCAGCTG TCTGCTTAGG CAGCACAAAT 
ACCAACAACA GTGCAACTAC AATGGCCACT GCTGGTGGTA TAGTGGGTTT TAGTAATGCC 
TCTTTGGGTG CAGGAATTGG ATCTCAGACC CAGCCTCAAG GCCAAATCAT GCAGAATGTC 
ATCATCCAGC GAACGCCAAC GCCAATACAG CCCAAACCTC CTCAAGGGGG TACAATCCAA 
CCCAAAATCT TCAAGCAACA ACAACAACAG CTTCCTGCCC CACATGCCTT GCCAAATGAT 
GCTAATAAAG CTCTGGGGGT ACAGCAAATC CCAGTGTCAG CTGGTCAGAA TGTAACGTTC 
CTCACCGGGA AGCCTGGCTC TAACGTAGTT TTGAGCACAC AGGCAGCGTC GCAGGGAACA 
CAGTTTTCTC AAGCGCTCTT TAAACAGCAG GGCCCTCAAA CTTCAGGCAA ACCCCTCAGT 
GTTCACTTGT TAAACCAACA GGGCAGCATT GTCATTCCCT CGCAAACTGT CCTGCAGGGC 
CAAAACCACC AGTTCCTCTT ACCAGGGCTT CAAGCAGGAG GCCAGATCCT GGCTCAGCAT 
CCTGGTGGAC ACATCATAAC CAGTCAGGGG CCTGGAGGGC AGATAATCGC TAATCAGATC 
TTGACAGCCA ATCAGAACAT CAACTTGGGA CAGGTGTTGG CTTCACAGGG CCACCCTGGT 
GCAGCCCACA TTCTCTCTGG ACACATCCAG CTCCAGCCTG GTCAGATGGG TCAGCCTGCA 
CTGTTCCAAA TGCCGGTGTC TTTGGCGCAT ACTCAGACAC AATCTCATCC GGTAACCGGG 
CATGTACAAA CGGTCATTCA GGGCATGCCT ATACAGAATT CCCTGTCTGT GGAGAGCCTT 
AGCCCAGCAG TCAGTTTACA GACGTTGCAG CAGTCTGGCG GCGTCCCTAA TAATGGTAGC 
AGTGGATCGA CGGCAATGGC GCCATGCCAG CCAGGAGAGG GTATCACTGT ATTGGGCGGC 
TCTTCTGACT CTGCTGCTCA ACCAGCACAG ACGCAACCTC AGCCCTCAAT TCTCACGGTT 
CAGACGACGC CTCCAGTTTC AGTAGCAGCT TCGGTTCCTT CCTCTTCATC TCCATCCCCA 
ACGATGGCCA CGTCTGCATC CACAATGGTG GGTCTGTGTT CACAGGCCCA GCACAGCCCT 
GGAAAAGTGT TGTTCACGTC ACCTGGTTCC GGCATGATCC TGAGCCAGGA GCCTCTTCAA 
ATGTTCCTGC ACCAGGAGCA ACAGCATCAC GCAGGAAAAG ACCCACCTGC CGCTGTGGGT 
GTACCTGCAT CTGTTATCGT CAGCGGCAGC AGTTCTGGTC CCGTCCCTTC AGGCCATGAC 
AACTTGTTAG CTGAGACTCG GCAGAGGCAG AGTCCCAGTC CCTCCCTTGG TCCTGCCCAC 
ATGGCAACAG TGGTTAACAA GGTTCCATCG TCAGCACATT CACAGTCTAT AAAGATCCAA 
AACGCCTCCC AATCTCAGCC TGTGGTCACC CCTTCGCCAG CACCCACACT GTCCGACAGC 
CCTCAACCTG CTCAAGTTTC CCCTCTCACT CTCGGGCAGC AGATCCAGTC TCCCCATCAA 
CAGTCTCGGC CTCCCTCGCA GCCCCAGCCT CCTTCACAGG CACAGACTCC TTCACGCTCA 
TGCACCCCTT CCTCTCTACC GCCTCTCTTC ATCATTCACA ATCAGATGGG AGGTTCTCCA 
CAGCCGGCTC CACCACCTCA GCAACAGCAG CCTCAGCAGT TACAAGTGCA GCTCCAGCCT 
CAAGTTCTTC CTCAGCCTGC TGCTCTGCAG CCAGACGTAC CTCCTTCCTC GTGCTCCCCA 
AAGCCTCCGC AGCCACTTCC CACACAGTTC CAGTTCCAGC CCCCAGTCTC CAGCTCGTCT 
CCAGCTGCAG CAGTGAAACA GCAGGTGACG GTGGTGCCTG GGCTGACCGC AGAACAGCAG 
CATCACCTAC AGCTGGTCAG TGCGCAGCTG CAGACCATGT CATCAATCAC ACAGCCCTCC 
CCTCAACAAA AGCAGCTCTT GGAAAAACTT CACCAGGTCC AGCAAAACAT ACTTTTGCAG 
GCTAAGCAGC AAGCTCAGGC ACAAGCCCAG GCCTCTCAGC AACAGGCTAC AAACCAGTTC 
AACAAAATGC CAGATCAGCC TTCGGTCCAA GCTGCGACTT CTTCAACGGC CACTGGAGCC 
ATCCAAGCAC CAGTCCAGTC TCTTCTGCCG CAGAAGTCTG TGCTTGTCAA GCCCTCTACT 
ACAGGTGCAA ATGACACACA AGTGTTTTCT GCGGTGTCTG CTGGGGCTAC GGTGAACACG 
GGAATCACAA CACCAAACCT TGCACAGGCT GTCCAGGCAA AGCCAGGCGT CATAAGTTCG 
GTTAGTGGAC TAACTCTGGG CAAAGCAGGT TTGCAGATTC AGGTGTTAGG TGCTGGACTC 
TCTCAAATGC CTGCACCTCC ACTACCTGCT CCCCAAACAC AGACATCAAC ATTAAAAAGG 
CCCTTTAGTA TGGAACCAAG CAAAGAAGCT AGGATGCTGG AACAGCTGCG AAAACAGCAA 
GGCTCAGTTC TCCATCCTGA CTACAGCTCT CCTTTCCGCT CTTTTGAGGA CACACTTCAT 
CGGCTGCTGC CATACCATCT GTACCAAGGC ACGGCTTCAT CTCCGGAAGA CTGTCGTAAA 
GTGGATGATG AATTTGAGAA CGTCTCAAGC CAGCTCTTGA AACGCACACA AGCAATGCTG 
GATAAATATC GTCACTTGCT TTTCGAGGAA TCAAAGCAGA GGCTGGGTCC CTCAGCAGAG 
ATGGTTATGA TTGACCGGAT GTTCATTCAA GAGGAAAAGG TTGCTCTGAG TCAGGACAGG 
GTCCTAGCCA AGGAGAAACC AGAGGAATTT GTGGCCAATT CGTGTTTAGT GGACAGCAGT 
GCGGTGAAGC TTGAAAAGGT GGAGCTAAGT TCTGTAAGAA CTGCATCAAC AATGGTTGCG 
GCTCCGCCTA CAGCAGTTAC GCCTGCTCCT GCCACAACTG TTGCCCCGGC TCCAACCTCT 
ACACCTGCTC CAGCTGCTGC CCCAACTCCT GCTCCTGCTC CTGCCCCTGC ACTCTTCCCT 
CCAACAAAAC TGGTGATCAA GCAAGGTGGA GGAGGAGCTT CAGTCTCCTG GTCCACCAGC 
TCAACCCCTA CGCCTGCTCC GGTGGTGCGA CCGCCTGCTG AGCCTGTCGC ACCAAGTGCC 
TCCTTCAGCC GCACTCCGTC CTCCCGCCCT GCTGACGACG ACGATGACGT TGCACTTCCC 
CAACGGACCA GCAAACCACC CATCAAGACC TACGAGGCAC GTCGGCGGAT AGGTTTGAAG 
CTGAAGATCA AGCAAGAGGC GGGGTTAAGT AAGGTGGTTC ACAACACTGC ATTAGATCCT 
GTCCATTCGC AATCACAACT CACACCACAG ACGCCCACCC CCGAGCAGCC GCAGAAAGTA 
AAGCCACACG TAACTCTTCC CTCCACCGTC ATTAGAACTC AACCCACAAC CTCTCAATCC 
ACCTCCTCCT CCACTGTCAC CACAGTAACC ACACACACAG CCTCCGCCTC CAGTAGCGCA 
ACTTCCTCGA GCGGGGCATC GTCTTCATTC TCCACCTGGT CTTCATCGTC ACCTTCTACG 
TCGACCGCGC AGATGAACGG CACCCTGGAG CACCATGAGG TAGGTGGGGT CAAACGTAAC 
CCAGCGTCCA CAGCAACGCC ACCACTAACA ACGTGCAGGC TCCCGCTCCG AAAGACATAC 
CGTGAGAACA TCAGTCCCCG TCACAGGCCT GGAGTACCAG GAGGTGGTGG AGATACACTA 
CCCATTCTGC CTGCAGGACC TCCTGTTACC TCTTCGCCCC AACCGCAAAG CTCGTCCCCT 
CAACCCGAAC GAACTGTGAT AGCCAGTGTG AAACTGGAGC GGCAAGGTGG ACACGGGCGA 
TCTCACCCCC ATGCGGAGTC ACAAAGCCTG GCAGCCGTTG AAGACGTCCT TTATCGGGGA 
ATAAAAAACG CGTACCAACA TCATCGGGAG TTTGATAAAG ACGATGAGGA TGATATGGAG 
GAAGGTGGTG GTGGTGGTGG TGCTTTGGGG CGGTTAAAGG GCATAGGAAG CAAACACCGG 
GAAGGGGGAC GGGGCGCCTT CAGGATGGAT CAGCATGCCC CAGGGCCGCC CTCTCCCGGA 
GAGTCTTCCT GCACAAGAGA CTCGTCACTT CCTGCCAAAC GCTGCAAGTC GGACTCTCCG 
GACATGGACA ATGCAAGCTT CTCCAGCGGC AGCCCGCCGC CTGACGACTC GCTAAATGAG 
CACCTGCAGT GTGCCATAGA CAGTATACTA AACCTCCAAC AGGGCCCTCC TGGACACGGA 
GGCTCAGGGA GAGGGGATTT AGGGAGGGTT CATGGGGCAA ACCTGCCTAA CCAGCACCAA 
ACCCATCCCT CCTACAGACA GTCCATGCCT CCCCTCTCAA CACAGCCCTC GTCCGCCCCC 
ATGTCCCAGC ATTCACAGGT AGGAGGGCGA GGGCAGAATG GAAATCTGGT TTCACAGACG 
CACAGTAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159309.1
CDS	1149..6620
Translation	MDDEDGRCLLDVICDPQALNDFLHGSETQLDTDDLLDGSGDPSSSFFSSTGGHVPEVPAQVQLSTSEPGLPRVSVDLDFLEDDDILGGSPGGDNGSNGVGTNHEPCDILQQSLAEANITEQSLQEADAELDLSSFGLTGLTQVVQPLPDAGLSGVGIGGATQIFPNQGTPTAPSNPTPDMLGSVLAHPGLQLQQQVMNKAISVQPFVQQVGLGNVTLQPISSLQALPNGSQSGPLGIGQIQVVGQPTVMTINPSGQQILTKTMGGYQLHQPGPEAASAGTQAGLGSSVLSSGGGLVIQGGKATLGSPALNGPAVCLGSTNTNNSATTMATAGGIVGFSNASLGAGIGSQTQPQGQIMQNVIIQRTPTPIQPKPPQGGTIQPKIFKQQQQQLPAPHALPNDANKALGVQQIPVSAGQNVTFLTGKPGSNVVLSTQAASQGTQFSQALFKQQGPQTSGKPLSVHLLNQQGSIVIPSQTVLQGQNHQFLLPGLQAGGQILAQHPGGHIITSQGPGGQIIANQILTANQNINLGQVLASQGHPGAAHILSGHIQLQPGQMGQPALFQMPVSLAHTQTQSHPVTGHVQTVIQGMPIQNSLSVESLSPAVSLQTLQQSGGVPNNGSSGSTAMAPCQPGEGITVLGGSSDSAAQPAQTQPQPSILTVQTTPPVSVAASVPSSSSPSPTMATSASTMVGLCSQAQHSPGKVLFTSPGSGMILSQEPLQMFLHQEQQHHAGKDPPAAVGVPASVIVSGSSSGPVPSGHDNLLAETRQRQSPSPSLGPAHMATVVNKVPSSAHSQSIKIQNASQSQPVVTPSPAPTLSDSPQPAQVSPLTLGQQIQSPHQQSRPPSQPQPPSQAQTPSRSCTPSSLPPLFIIHNQMGGSPQPAPPPQQQQPQQLQVQLQPQVLPQPAALQPDVPPSSCSPKPPQPLPTQFQFQPPVSSSSPAAAVKQQVTVVPGLTAEQQHHLQLVSAQLQTMSSITQPSPQQKQLLEKLHQVQQNILLQAKQQAQAQAQASQQQATNQFNKMPDQPSVQAATSSTATGAIQAPVQSLLPQKSVLVKPSTTGANDTQVFSAVSAGATVNTGITTPNLAQAVQAKPGVISSVSGLTLGKAGLQIQVLGAGLSQMPAPPLPAPQTQTSTLKRPFSMEPSKEARMLEQLRKQQGSVLHPDYSSPFRSFEDTLHRLLPYHLYQGTASSPEDCRKVDDEFENVSSQLLKRTQAMLDKYRHLLFEESKQRLGPSAEMVMIDRMFIQEEKVALSQDRVLAKEKPEEFVANSCLVDSSAVKLEKVELSSVRTASTMVAAPPTAVTPAPATTVAPAPTSTPAPAAAPTPAPAPAPALFPPTKLVIKQGGGGASVSWSTSSTPTPAPVVRPPAEPVAPSASFSRTPSSRPADDDDDVALPQRTSKPPIKTYEARRRIGLKLKIKQEAGLSKVVHNTALDPVHSQSQLTPQTPTPEQPQKVKPHVTLPSTVIRTQPTTSQSTSSSTVTTVTTHTASASSSATSSSGASSSFSTWSSSSPSTSTAQMNGTLEHHEVGGVKRNPASTATPPLTTCRLPLRKTYRENISPRHRPGVPGGGGDTLPILPAGPPVTSSPQPQSSSPQPERTVIASVKLERQGGHGRSHPHAESQSLAAVEDVLYRGIKNAYQHHREFDKDDEDDMEEGGGGGGALGRLKGIGSKHREGGRGAFRMDQHAPGPPSPGESSCTRDSSLPAKRCKSDSPDMDNASFSSGSPPPDDSLNEHLQCAIDSILNLQQGPPGHGGSGRGDLGRVHGANLPNQHQTHPSYRQSMPPLSTQPSSAPMSQHSQVGGRGQNGNLVSQTHSR

Target ORF information:

RefSeq Version	XM_005159252.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio glioma tumor suppressor candidate region gene 1 (gltscr1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159252.3

ORF Insert Sequence:

ATGGATGATG AAGATGGCAG GTGCTTGCTA GATGTAATTT GTGACCCACA AGCACTCAAC 
GATTTTCTTC ATGGATCAGA AACACAACTG GACACTGATG ACCTATTGGA TGGCTCAGGT 
GACCCGTCCA GCTCGTTCTT CTCCAGCACT GGGGGCCATG TTCCTGAGGT TCCGGCACAG 
GTTCAGCTGT CGACAAGTGA GCCGGGCCTT CCAAGAGTCA GCGTTGATTT GGACTTCTTG 
GAGGACGATG ACATATTGGG GGGATCGCCA GGAGGAGACA ATGGAAGTAA TGGTGTTGGG 
ACAAATCATG AGCCCTGTGA CATTTTGCAG CAGAGCTTGG CGGAGGCTAA CATCACAGAG 
CAGAGCCTTC AAGAGGCAGA TGCTGAGCTT GACCTCAGTT CCTTTGGGCT AACTGGCCTT 
ACGCAGGTGG TTCAACCCCT GCCTGATGCC GGCCTGTCTG GGGTGGGCAT TGGAGGTGCA 
ACTCAGATTT TTCCAAACCA AGGCACCCCT ACCGCACCCT CAAATCCCAC TCCAGACATG 
CTTGGTTCTG TCCTGGCACA TCCAGGTCTG CAGCTTCAAC AACAGGTCAT GAACAAGGCT 
ATCAGCGTCC AGCCTTTTGT ACAACAAGTC GGACTTGGAA ATGTAACACT TCAGCCAATT 
TCGAGCCTTC AGGCCCTACC GAACGGCAGC CAGTCTGGAC CGTTGGGCAT TGGACAAATT 
CAAGTGGTGG GTCAGCCCAC TGTAATGACT ATAAATCCAT CTGGGCAGCA AATCTTGACG 
AAAACCATGG GTGGCTACCA ACTGCATCAA CCTGGGCCGG AGGCAGCAAG CGCTGGAACG 
CAGGCTGGGC TTGGAAGCTC TGTTTTGAGT TCAGGAGGTG GACTTGTAAT TCAAGGGGGC 
AAGGCCACTC TCGGGTCCCC TGCCTTAAAT GGGCCAGCTG TCTGCTTAGG CAGCACAAAT 
ACCAACAACA GTGCAACTAC AATGGCCACT GCTGGTGGTA TAGTGGGTTT TAGTAATGCC 
TCTTTGGGTG CAGGAATTGG ATCTCAGACC CAGCCTCAAG GCCAAATCAT GCAGAATGTC 
ATCATCCAGC GAACGCCAAC GCCAATACAG CCCAAACCTC CTCAAGGGGG TACAATCCAA 
CCCAAAATCT TCAAGCAACA ACAACAACAG CTTCCTGCCC CACATGCCTT GCCAAATGAT 
GCTAATAAAG CTCTGGGGGT ACAGCAAATC CCAGTGTCAG CTGGTCAGAA TGTAACGTTC 
CTCACCGGGA AGCCTGGCTC TAACGTAGTT TTGAGCACAC AGGCAGCGTC GCAGGGAACA 
CAGTTTTCTC AAGCGCTCTT TAAACAGCAG GGCCCTCAAA CTTCAGGCAA ACCCCTCAGT 
GTTCACTTGT TAAACCAACA GGGCAGCATT GTCATTCCCT CGCAAACTGT CCTGCAGGGC 
CAAAACCACC AGTTCCTCTT ACCAGGGCTT CAAGCAGGAG GCCAGATCCT GGCTCAGCAT 
CCTGGTGGAC ACATCATAAC CAGTCAGGGG CCTGGAGGGC AGATAATCGC TAATCAGATC 
TTGACAGCCA ATCAGAACAT CAACTTGGGA CAGGTGTTGG CTTCACAGGG CCACCCTGGT 
GCAGCCCACA TTCTCTCTGG ACACATCCAG CTCCAGCCTG GTCAGATGGG TCAGCCTGCA 
CTGTTCCAAA TGCCGGTGTC TTTGGCGCAT ACTCAGACAC AATCTCATCC GGTAACCGGG 
CATGTACAAA CGGTCATTCA GGGCATGCCT ATACAGAATT CCCTGTCTGT GGAGAGCCTT 
AGCCCAGCAG TCAGTTTACA GACGTTGCAG CAGTCTGGCG GCGTCCCTAA TAATGGTAGC 
AGTGGATCGA CGGCAATGGC GCCATGCCAG CCAGGAGAGG GTATCACTGT ATTGGGCGGC 
TCTTCTGACT CTGCTGCTCA ACCAGCACAG ACGCAACCTC AGCCCTCAAT TCTCACGGTT 
CAGACGACGC CTCCAGTTTC AGTAGCAGCT TCGGTTCCTT CCTCTTCATC TCCATCCCCA 
ACGATGGCCA CGTCTGCATC CACAATGGTG GGTCTGTGTT CACAGGCCCA GCACAGCCCT 
GGAAAAGTGT TGTTCACGTC ACCTGGTTCC GGCATGATCC TGAGCCAGGA GCCTCTTCAA 
ATGTTCCTGC ACCAGGAGCA ACAGCATCAC GCAGGAAAAG ACCCACCTGC CGCTGTGGGT 
GTACCTGCAT CTGTTATCGT CAGCGGCAGC AGTTCTGGTC CCGTCCCTTC AGGCCATGAC 
AACTTGTTAG CTGAGACTCG GCAGAGGCAG AGTCCCAGTC CCTCCCTTGG TCCTGCCCAC 
ATGGCAACAG TGGTTAACAA GGTTCCATCG TCAGCACATT CACAGTCTAT AAAGATCCAA 
AACGCCTCCC AATCTCAGCC TGTGGTCACC CCTTCGCCAG CACCCACACT GTCCGACAGC 
CCTCAACCTG CTCAAGTTTC CCCTCTCACT CTCGGGCAGC AGATCCAGTC TCCCCATCAA 
CAGTCTCGGC CTCCCTCGCA GCCCCAGCCT CCTTCACAGG CACAGACTCC TTCACGCTCA 
TGCACCCCTT CCTCTCTACC GCCTCTCTTC ATCATTCACA ATCAGATGGG AGGTTCTCCA 
CAGCCGGCTC CACCACCTCA GCAACAGCAG CCTCAGCAGT TACAAGTGCA GCTCCAGCCT 
CAAGTTCTTC CTCAGCCTGC TGCTCTGCAG CCAGACGTAC CTCCTTCCTC GTGCTCCCCA 
AAGCCTCCGC AGCCACTTCC CACACAGTTC CAGTTCCAGC CCCCAGTCTC CAGCTCGTCT 
CCAGCTGCAG CAGTGAAACA GCAGGTGACG GTGGTGCCTG GGCTGACCGC AGAACAGCAG 
CATCACCTAC AGCTGGTCAG TGCGCAGCTG CAGACCATGT CATCAATCAC ACAGCCCTCC 
CCTCAACAAA AGCAGCTCTT GGAAAAACTT CACCAGGTCC AGCAAAACAT ACTTTTGCAG 
GCTAAGCAGC AAGCTCAGGC ACAAGCCCAG GCCTCTCAGC AACAGGCTAC AAACCAGTTC 
AACAAAATGC CAGATCAGCC TTCGGTCCAA GCTGCGACTT CTTCAACGGC CACTGGAGCC 
ATCCAAGCAC CAGTCCAGTC TCTTCTGCCG CAGAAGTCTG TGCTTGTCAA GCCCTCTACT 
ACAGGTGCAA ATGACACACA AGTGTTTTCT GCGGTGTCTG CTGGGGCTAC GGTGAACACG 
GGAATCACAA CACCAAACCT TGCACAGGCT GTCCAGGCAA AGCCAGGCGT CATAAGTTCG 
GTTAGTGGAC TAACTCTGGG CAAAGCAGGT TTGCAGATTC AGGTGTTAGG TGCTGGACTC 
TCTCAAATGC CTGCACCTCC ACTACCTGCT CCCCAAACAC AGACATCAAC ATTAAAAAGG 
CCCTTTAGTA TGGAACCAAG CAAAGAAGCT AGGATGCTGG AACAGCTGCG AAAACAGCAA 
GGCTCAGTTC TCCATCCTGA CTACAGCTCT CCTTTCCGCT CTTTTGAGGA CACACTTCAT 
CGGCTGCTGC CATACCATCT GTACCAAGGC ACGGCTTCAT CTCCGGAAGA CTGTCGTAAA 
GTGGATGATG AATTTGAGAA CGTCTCAAGC CAGCTCTTGA AACGCACACA AGCAATGCTG 
GATAAATATC GTCACTTGCT TTTCGAGGAA TCAAAGCAGA GGCTGGGTCC CTCAGCAGAG 
ATGGTTATGA TTGACCGGAT GTTCATTCAA GAGGAAAAGG TTGCTCTGAG TCAGGACAGG 
GTCCTAGCCA AGGAGAAACC AGAGGAATTT GTGGCCAATT CGTGTTTAGT GGACAGCAGT 
GCGGTGAAGC TTGAAAAGGT GGAGCTAAGT TCTGTAAGAA CTGCATCAAC AATGGTTGCG 
GCTCCGCCTA CAGCAGTTAC GCCTGCTCCT GCCACAACTG TTGCCCCGGC TCCAACCTCT 
ACACCTGCTC CAGCTGCTGC CCCAACTCCT GCTCCTGCTC CTGCCCCTGC ACTCTTCCCT 
CCAACAAAAC TGGTGATCAA GCAAGGTGGA GGAGGAGCTT CAGTCTCCTG GTCCACCAGC 
TCAACCCCTA CGCCTGCTCC GGTGGTGCGA CCGCCTGCTG AGCCTGTCGC ACCAAGTGCC 
TCCTTCAGCC GCACTCCGTC CTCCCGCCCT GCTGACGACG ACGATGACGT TGCACTTCCC 
CAACGGACCA GCAAACCACC CATCAAGACC TACGAGGCAC GTCGGCGGAT AGGTTTGAAG 
CTGAAGATCA AGCAAGAGGC GGGGTTAAGT AAGGTGGTTC ACAACACTGC ATTAGATCCT 
GTCCATTCGC AATCACAACT CACACCACAG ACGCCCACCC CCGAGCAGCC GCAGAAAGTA 
AAGCCACACG TAACTCTTCC CTCCACCGTC ATTAGAACTC AACCCACAAC CTCTCAATCC 
ACCTCCTCCT CCACTGTCAC CACAGTAACC ACACACACAG CCTCCGCCTC CAGTAGCGCA 
ACTTCCTCGA GCGGGGCATC GTCTTCATTC TCCACCTGGT CTTCATCGTC ACCTTCTACG 
TCGACCGCGC AGATGAACGG CACCCTGGAG CACCATGAGG TAGGTGGGGT CAAACGTAAC 
CCAGCGTCCA CAGCAACGCC ACCACTAACA ACGTGCAGGC TCCCGCTCCG AAAGACATAC 
CGTGAGAACA TCAGTCCCCG TCACAGGCCT GGAGTACCAG GAGGTGGTGG AGATACACTA 
CCCATTCTGC CTGCAGGACC TCCTGTTACC TCTTCGCCCC AACCGCAAAG CTCGTCCCCT 
CAACCCGAAC GAACTGTGAT AGCCAGTGTG AAACTGGAGC GGCAAGGTGG ACACGGGCGA 
TCTCACCCCC ATGCGGAGTC ACAAAGCCTG GCAGCCGTTG AAGACGTCCT TTATCGGGGA 
ATAAAAAACG CGTACCAACA TCATCGGGAG TTTGATAAAG ACGATGAGGA TGATATGGAG 
GAAGGTGGTG GTGGTGGTGG TGCTTTGGGG CGGTTAAAGG GCATAGGAAG CAAACACCGG 
GAAGGGGGAC GGGGCGCCTT CAGGATGGAT CAGCATGCCC CAGGGCCGCC CTCTCCCGGA 
GAGTCTTCCT GCACAAGAGA CTCGTCACTT CCTGCCAAAC GCTGCAAGTC GGACTCTCCG 
GACATGGACA ATGCAAGCTT CTCCAGCGGC AGCCCGCCGC CTGACGACTC GCTAAATGAG 
CACCTGCAGT GTGCCATAGA CAGTATACTA AACCTCCAAC AGGGCCCTCC TGGACACGGA 
GGCTCAGGGA GAGGGGATTT AGGGAGGGTT CATGGGGCAA ACCTGCCTAA CCAGCACCAA 
ACCCATCCCT CCTACAGACA GTCCATGCCT CCCCTCTCAA CACAGCCCTC GTCCGCCCCC 
ATGTCCCAGC ATTCACAGGT AGGAGGGCGA GGGCAGAATG GAAATCTGGT TTCACAGACG 
CACAGTAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bicra ( XM_005159252.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159309.1 Danio rerio BRD4 interacting chromatin remodeling complex associated protein (bicra), transcript variant X1, mRNA.

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CloneID	ODa23558
Clone ID Related Accession (Same CDS sequence)	XM_005159252.3 , XM_005159252.4
Accession Version	XM_005159252.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	glioma tumor suppressor candidate region gene 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159252.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATG AAGATGGCAG GTGCTTGCTA GATGTAATTT GTGACCCACA AGCACTCAAC 
GATTTTCTTC ATGGATCAGA AACACAACTG GACACTGATG ACCTATTGGA TGGCTCAGGT 
GACCCGTCCA GCTCGTTCTT CTCCAGCACT GGGGGCCATG TTCCTGAGGT TCCGGCACAG 
GTTCAGCTGT CGACAAGTGA GCCGGGCCTT CCAAGAGTCA GCGTTGATTT GGACTTCTTG 
GAGGACGATG ACATATTGGG GGGATCGCCA GGAGGAGACA ATGGAAGTAA TGGTGTTGGG 
ACAAATCATG AGCCCTGTGA CATTTTGCAG CAGAGCTTGG CGGAGGCTAA CATCACAGAG 
CAGAGCCTTC AAGAGGCAGA TGCTGAGCTT GACCTCAGTT CCTTTGGGCT AACTGGCCTT 
ACGCAGGTGG TTCAACCCCT GCCTGATGCC GGCCTGTCTG GGGTGGGCAT TGGAGGTGCA 
ACTCAGATTT TTCCAAACCA AGGCACCCCT ACCGCACCCT CAAATCCCAC TCCAGACATG 
CTTGGTTCTG TCCTGGCACA TCCAGGTCTG CAGCTTCAAC AACAGGTCAT GAACAAGGCT 
ATCAGCGTCC AGCCTTTTGT ACAACAAGTC GGACTTGGAA ATGTAACACT TCAGCCAATT 
TCGAGCCTTC AGGCCCTACC GAACGGCAGC CAGTCTGGAC CGTTGGGCAT TGGACAAATT 
CAAGTGGTGG GTCAGCCCAC TGTAATGACT ATAAATCCAT CTGGGCAGCA AATCTTGACG 
AAAACCATGG GTGGCTACCA ACTGCATCAA CCTGGGCCGG AGGCAGCAAG CGCTGGAACG 
CAGGCTGGGC TTGGAAGCTC TGTTTTGAGT TCAGGAGGTG GACTTGTAAT TCAAGGGGGC 
AAGGCCACTC TCGGGTCCCC TGCCTTAAAT GGGCCAGCTG TCTGCTTAGG CAGCACAAAT 
ACCAACAACA GTGCAACTAC AATGGCCACT GCTGGTGGTA TAGTGGGTTT TAGTAATGCC 
TCTTTGGGTG CAGGAATTGG ATCTCAGACC CAGCCTCAAG GCCAAATCAT GCAGAATGTC 
ATCATCCAGC GAACGCCAAC GCCAATACAG CCCAAACCTC CTCAAGGGGG TACAATCCAA 
CCCAAAATCT TCAAGCAACA ACAACAACAG CTTCCTGCCC CACATGCCTT GCCAAATGAT 
GCTAATAAAG CTCTGGGGGT ACAGCAAATC CCAGTGTCAG CTGGTCAGAA TGTAACGTTC 
CTCACCGGGA AGCCTGGCTC TAACGTAGTT TTGAGCACAC AGGCAGCGTC GCAGGGAACA 
CAGTTTTCTC AAGCGCTCTT TAAACAGCAG GGCCCTCAAA CTTCAGGCAA ACCCCTCAGT 
GTTCACTTGT TAAACCAACA GGGCAGCATT GTCATTCCCT CGCAAACTGT CCTGCAGGGC 
CAAAACCACC AGTTCCTCTT ACCAGGGCTT CAAGCAGGAG GCCAGATCCT GGCTCAGCAT 
CCTGGTGGAC ACATCATAAC CAGTCAGGGG CCTGGAGGGC AGATAATCGC TAATCAGATC 
TTGACAGCCA ATCAGAACAT CAACTTGGGA CAGGTGTTGG CTTCACAGGG CCACCCTGGT 
GCAGCCCACA TTCTCTCTGG ACACATCCAG CTCCAGCCTG GTCAGATGGG TCAGCCTGCA 
CTGTTCCAAA TGCCGGTGTC TTTGGCGCAT ACTCAGACAC AATCTCATCC GGTAACCGGG 
CATGTACAAA CGGTCATTCA GGGCATGCCT ATACAGAATT CCCTGTCTGT GGAGAGCCTT 
AGCCCAGCAG TCAGTTTACA GACGTTGCAG CAGTCTGGCG GCGTCCCTAA TAATGGTAGC 
AGTGGATCGA CGGCAATGGC GCCATGCCAG CCAGGAGAGG GTATCACTGT ATTGGGCGGC 
TCTTCTGACT CTGCTGCTCA ACCAGCACAG ACGCAACCTC AGCCCTCAAT TCTCACGGTT 
CAGACGACGC CTCCAGTTTC AGTAGCAGCT TCGGTTCCTT CCTCTTCATC TCCATCCCCA 
ACGATGGCCA CGTCTGCATC CACAATGGTG GGTCTGTGTT CACAGGCCCA GCACAGCCCT 
GGAAAAGTGT TGTTCACGTC ACCTGGTTCC GGCATGATCC TGAGCCAGGA GCCTCTTCAA 
ATGTTCCTGC ACCAGGAGCA ACAGCATCAC GCAGGAAAAG ACCCACCTGC CGCTGTGGGT 
GTACCTGCAT CTGTTATCGT CAGCGGCAGC AGTTCTGGTC CCGTCCCTTC AGGCCATGAC 
AACTTGTTAG CTGAGACTCG GCAGAGGCAG AGTCCCAGTC CCTCCCTTGG TCCTGCCCAC 
ATGGCAACAG TGGTTAACAA GGTTCCATCG TCAGCACATT CACAGTCTAT AAAGATCCAA 
AACGCCTCCC AATCTCAGCC TGTGGTCACC CCTTCGCCAG CACCCACACT GTCCGACAGC 
CCTCAACCTG CTCAAGTTTC CCCTCTCACT CTCGGGCAGC AGATCCAGTC TCCCCATCAA 
CAGTCTCGGC CTCCCTCGCA GCCCCAGCCT CCTTCACAGG CACAGACTCC TTCACGCTCA 
TGCACCCCTT CCTCTCTACC GCCTCTCTTC ATCATTCACA ATCAGATGGG AGGTTCTCCA 
CAGCCGGCTC CACCACCTCA GCAACAGCAG CCTCAGCAGT TACAAGTGCA GCTCCAGCCT 
CAAGTTCTTC CTCAGCCTGC TGCTCTGCAG CCAGACGTAC CTCCTTCCTC GTGCTCCCCA 
AAGCCTCCGC AGCCACTTCC CACACAGTTC CAGTTCCAGC CCCCAGTCTC CAGCTCGTCT 
CCAGCTGCAG CAGTGAAACA GCAGGTGACG GTGGTGCCTG GGCTGACCGC AGAACAGCAG 
CATCACCTAC AGCTGGTCAG TGCGCAGCTG CAGACCATGT CATCAATCAC ACAGCCCTCC 
CCTCAACAAA AGCAGCTCTT GGAAAAACTT CACCAGGTCC AGCAAAACAT ACTTTTGCAG 
GCTAAGCAGC AAGCTCAGGC ACAAGCCCAG GCCTCTCAGC AACAGGCTAC AAACCAGTTC 
AACAAAATGC CAGATCAGCC TTCGGTCCAA GCTGCGACTT CTTCAACGGC CACTGGAGCC 
ATCCAAGCAC CAGTCCAGTC TCTTCTGCCG CAGAAGTCTG TGCTTGTCAA GCCCTCTACT 
ACAGGTGCAA ATGACACACA AGTGTTTTCT GCGGTGTCTG CTGGGGCTAC GGTGAACACG 
GGAATCACAA CACCAAACCT TGCACAGGCT GTCCAGGCAA AGCCAGGCGT CATAAGTTCG 
GTTAGTGGAC TAACTCTGGG CAAAGCAGGT TTGCAGATTC AGGTGTTAGG TGCTGGACTC 
TCTCAAATGC CTGCACCTCC ACTACCTGCT CCCCAAACAC AGACATCAAC ATTAAAAAGG 
CCCTTTAGTA TGGAACCAAG CAAAGAAGCT AGGATGCTGG AACAGCTGCG AAAACAGCAA 
GGCTCAGTTC TCCATCCTGA CTACAGCTCT CCTTTCCGCT CTTTTGAGGA CACACTTCAT 
CGGCTGCTGC CATACCATCT GTACCAAGGC ACGGCTTCAT CTCCGGAAGA CTGTCGTAAA 
GTGGATGATG AATTTGAGAA CGTCTCAAGC CAGCTCTTGA AACGCACACA AGCAATGCTG 
GATAAATATC GTCACTTGCT TTTCGAGGAA TCAAAGCAGA GGCTGGGTCC CTCAGCAGAG 
ATGGTTATGA TTGACCGGAT GTTCATTCAA GAGGAAAAGG TTGCTCTGAG TCAGGACAGG 
GTCCTAGCCA AGGAGAAACC AGAGGAATTT GTGGCCAATT CGTGTTTAGT GGACAGCAGT 
GCGGTGAAGC TTGAAAAGGT GGAGCTAAGT TCTGTAAGAA CTGCATCAAC AATGGTTGCG 
GCTCCGCCTA CAGCAGTTAC GCCTGCTCCT GCCACAACTG TTGCCCCGGC TCCAACCTCT 
ACACCTGCTC CAGCTGCTGC CCCAACTCCT GCTCCTGCTC CTGCCCCTGC ACTCTTCCCT 
CCAACAAAAC TGGTGATCAA GCAAGGTGGA GGAGGAGCTT CAGTCTCCTG GTCCACCAGC 
TCAACCCCTA CGCCTGCTCC GGTGGTGCGA CCGCCTGCTG AGCCTGTCGC ACCAAGTGCC 
TCCTTCAGCC GCACTCCGTC CTCCCGCCCT GCTGACGACG ACGATGACGT TGCACTTCCC 
CAACGGACCA GCAAACCACC CATCAAGACC TACGAGGCAC GTCGGCGGAT AGGTTTGAAG 
CTGAAGATCA AGCAAGAGGC GGGGTTAAGT AAGGTGGTTC ACAACACTGC ATTAGATCCT 
GTCCATTCGC AATCACAACT CACACCACAG ACGCCCACCC CCGAGCAGCC GCAGAAAGTA 
AAGCCACACG TAACTCTTCC CTCCACCGTC ATTAGAACTC AACCCACAAC CTCTCAATCC 
ACCTCCTCCT CCACTGTCAC CACAGTAACC ACACACACAG CCTCCGCCTC CAGTAGCGCA 
ACTTCCTCGA GCGGGGCATC GTCTTCATTC TCCACCTGGT CTTCATCGTC ACCTTCTACG 
TCGACCGCGC AGATGAACGG CACCCTGGAG CACCATGAGG TAGGTGGGGT CAAACGTAAC 
CCAGCGTCCA CAGCAACGCC ACCACTAACA ACGTGCAGGC TCCCGCTCCG AAAGACATAC 
CGTGAGAACA TCAGTCCCCG TCACAGGCCT GGAGTACCAG GAGGTGGTGG AGATACACTA 
CCCATTCTGC CTGCAGGACC TCCTGTTACC TCTTCGCCCC AACCGCAAAG CTCGTCCCCT 
CAACCCGAAC GAACTGTGAT AGCCAGTGTG AAACTGGAGC GGCAAGGTGG ACACGGGCGA 
TCTCACCCCC ATGCGGAGTC ACAAAGCCTG GCAGCCGTTG AAGACGTCCT TTATCGGGGA 
ATAAAAAACG CGTACCAACA TCATCGGGAG TTTGATAAAG ACGATGAGGA TGATATGGAG 
GAAGGTGGTG GTGGTGGTGG TGCTTTGGGG CGGTTAAAGG GCATAGGAAG CAAACACCGG 
GAAGGGGGAC GGGGCGCCTT CAGGATGGAT CAGCATGCCC CAGGGCCGCC CTCTCCCGGA 
GAGTCTTCCT GCACAAGAGA CTCGTCACTT CCTGCCAAAC GCTGCAAGTC GGACTCTCCG 
GACATGGACA ATGCAAGCTT CTCCAGCGGC AGCCCGCCGC CTGACGACTC GCTAAATGAG 
CACCTGCAGT GTGCCATAGA CAGTATACTA AACCTCCAAC AGGGCCCTCC TGGACACGGA 
GGCTCAGGGA GAGGGGATTT AGGGAGGGTT CATGGGGCAA ACCTGCCTAA CCAGCACCAA 
ACCCATCCCT CCTACAGACA GTCCATGCCT CCCCTCTCAA CACAGCCCTC GTCCGCCCCC 
ATGTCCCAGC ATTCACAGGT AGGAGGGCGA GGGCAGAATG GAAATCTGGT TTCACAGACG 
CACAGTAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159309.1
CDS	951..6422
Translation	MDDEDGRCLLDVICDPQALNDFLHGSETQLDTDDLLDGSGDPSSSFFSSTGGHVPEVPAQVQLSTSEPGLPRVSVDLDFLEDDDILGGSPGGDNGSNGVGTNHEPCDILQQSLAEANITEQSLQEADAELDLSSFGLTGLTQVVQPLPDAGLSGVGIGGATQIFPNQGTPTAPSNPTPDMLGSVLAHPGLQLQQQVMNKAISVQPFVQQVGLGNVTLQPISSLQALPNGSQSGPLGIGQIQVVGQPTVMTINPSGQQILTKTMGGYQLHQPGPEAASAGTQAGLGSSVLSSGGGLVIQGGKATLGSPALNGPAVCLGSTNTNNSATTMATAGGIVGFSNASLGAGIGSQTQPQGQIMQNVIIQRTPTPIQPKPPQGGTIQPKIFKQQQQQLPAPHALPNDANKALGVQQIPVSAGQNVTFLTGKPGSNVVLSTQAASQGTQFSQALFKQQGPQTSGKPLSVHLLNQQGSIVIPSQTVLQGQNHQFLLPGLQAGGQILAQHPGGHIITSQGPGGQIIANQILTANQNINLGQVLASQGHPGAAHILSGHIQLQPGQMGQPALFQMPVSLAHTQTQSHPVTGHVQTVIQGMPIQNSLSVESLSPAVSLQTLQQSGGVPNNGSSGSTAMAPCQPGEGITVLGGSSDSAAQPAQTQPQPSILTVQTTPPVSVAASVPSSSSPSPTMATSASTMVGLCSQAQHSPGKVLFTSPGSGMILSQEPLQMFLHQEQQHHAGKDPPAAVGVPASVIVSGSSSGPVPSGHDNLLAETRQRQSPSPSLGPAHMATVVNKVPSSAHSQSIKIQNASQSQPVVTPSPAPTLSDSPQPAQVSPLTLGQQIQSPHQQSRPPSQPQPPSQAQTPSRSCTPSSLPPLFIIHNQMGGSPQPAPPPQQQQPQQLQVQLQPQVLPQPAALQPDVPPSSCSPKPPQPLPTQFQFQPPVSSSSPAAAVKQQVTVVPGLTAEQQHHLQLVSAQLQTMSSITQPSPQQKQLLEKLHQVQQNILLQAKQQAQAQAQASQQQATNQFNKMPDQPSVQAATSSTATGAIQAPVQSLLPQKSVLVKPSTTGANDTQVFSAVSAGATVNTGITTPNLAQAVQAKPGVISSVSGLTLGKAGLQIQVLGAGLSQMPAPPLPAPQTQTSTLKRPFSMEPSKEARMLEQLRKQQGSVLHPDYSSPFRSFEDTLHRLLPYHLYQGTASSPEDCRKVDDEFENVSSQLLKRTQAMLDKYRHLLFEESKQRLGPSAEMVMIDRMFIQEEKVALSQDRVLAKEKPEEFVANSCLVDSSAVKLEKVELSSVRTASTMVAAPPTAVTPAPATTVAPAPTSTPAPAAAPTPAPAPAPALFPPTKLVIKQGGGGASVSWSTSSTPTPAPVVRPPAEPVAPSASFSRTPSSRPADDDDDVALPQRTSKPPIKTYEARRRIGLKLKIKQEAGLSKVVHNTALDPVHSQSQLTPQTPTPEQPQKVKPHVTLPSTVIRTQPTTSQSTSSSTVTTVTTHTASASSSATSSSGASSSFSTWSSSSPSTSTAQMNGTLEHHEVGGVKRNPASTATPPLTTCRLPLRKTYRENISPRHRPGVPGGGGDTLPILPAGPPVTSSPQPQSSSPQPERTVIASVKLERQGGHGRSHPHAESQSLAAVEDVLYRGIKNAYQHHREFDKDDEDDMEEGGGGGGALGRLKGIGSKHREGGRGAFRMDQHAPGPPSPGESSCTRDSSLPAKRCKSDSPDMDNASFSSGSPPPDDSLNEHLQCAIDSILNLQQGPPGHGGSGRGDLGRVHGANLPNQHQTHPSYRQSMPPLSTQPSSAPMSQHSQVGGRGQNGNLVSQTHSR

Target ORF information:

RefSeq Version	XM_005159252.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio BRD4 interacting chromatin remodeling complex associated protein (bicra), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159252.4

ORF Insert Sequence:

ATGGATGATG AAGATGGCAG GTGCTTGCTA GATGTAATTT GTGACCCACA AGCACTCAAC 
GATTTTCTTC ATGGATCAGA AACACAACTG GACACTGATG ACCTATTGGA TGGCTCAGGT 
GACCCGTCCA GCTCGTTCTT CTCCAGCACT GGGGGCCATG TTCCTGAGGT TCCGGCACAG 
GTTCAGCTGT CGACAAGTGA GCCGGGCCTT CCAAGAGTCA GCGTTGATTT GGACTTCTTG 
GAGGACGATG ACATATTGGG GGGATCGCCA GGAGGAGACA ATGGAAGTAA TGGTGTTGGG 
ACAAATCATG AGCCCTGTGA CATTTTGCAG CAGAGCTTGG CGGAGGCTAA CATCACAGAG 
CAGAGCCTTC AAGAGGCAGA TGCTGAGCTT GACCTCAGTT CCTTTGGGCT AACTGGCCTT 
ACGCAGGTGG TTCAACCCCT GCCTGATGCC GGCCTGTCTG GGGTGGGCAT TGGAGGTGCA 
ACTCAGATTT TTCCAAACCA AGGCACCCCT ACCGCACCCT CAAATCCCAC TCCAGACATG 
CTTGGTTCTG TCCTGGCACA TCCAGGTCTG CAGCTTCAAC AACAGGTCAT GAACAAGGCT 
ATCAGCGTCC AGCCTTTTGT ACAACAAGTC GGACTTGGAA ATGTAACACT TCAGCCAATT 
TCGAGCCTTC AGGCCCTACC GAACGGCAGC CAGTCTGGAC CGTTGGGCAT TGGACAAATT 
CAAGTGGTGG GTCAGCCCAC TGTAATGACT ATAAATCCAT CTGGGCAGCA AATCTTGACG 
AAAACCATGG GTGGCTACCA ACTGCATCAA CCTGGGCCGG AGGCAGCAAG CGCTGGAACG 
CAGGCTGGGC TTGGAAGCTC TGTTTTGAGT TCAGGAGGTG GACTTGTAAT TCAAGGGGGC 
AAGGCCACTC TCGGGTCCCC TGCCTTAAAT GGGCCAGCTG TCTGCTTAGG CAGCACAAAT 
ACCAACAACA GTGCAACTAC AATGGCCACT GCTGGTGGTA TAGTGGGTTT TAGTAATGCC 
TCTTTGGGTG CAGGAATTGG ATCTCAGACC CAGCCTCAAG GCCAAATCAT GCAGAATGTC 
ATCATCCAGC GAACGCCAAC GCCAATACAG CCCAAACCTC CTCAAGGGGG TACAATCCAA 
CCCAAAATCT TCAAGCAACA ACAACAACAG CTTCCTGCCC CACATGCCTT GCCAAATGAT 
GCTAATAAAG CTCTGGGGGT ACAGCAAATC CCAGTGTCAG CTGGTCAGAA TGTAACGTTC 
CTCACCGGGA AGCCTGGCTC TAACGTAGTT TTGAGCACAC AGGCAGCGTC GCAGGGAACA 
CAGTTTTCTC AAGCGCTCTT TAAACAGCAG GGCCCTCAAA CTTCAGGCAA ACCCCTCAGT 
GTTCACTTGT TAAACCAACA GGGCAGCATT GTCATTCCCT CGCAAACTGT CCTGCAGGGC 
CAAAACCACC AGTTCCTCTT ACCAGGGCTT CAAGCAGGAG GCCAGATCCT GGCTCAGCAT 
CCTGGTGGAC ACATCATAAC CAGTCAGGGG CCTGGAGGGC AGATAATCGC TAATCAGATC 
TTGACAGCCA ATCAGAACAT CAACTTGGGA CAGGTGTTGG CTTCACAGGG CCACCCTGGT 
GCAGCCCACA TTCTCTCTGG ACACATCCAG CTCCAGCCTG GTCAGATGGG TCAGCCTGCA 
CTGTTCCAAA TGCCGGTGTC TTTGGCGCAT ACTCAGACAC AATCTCATCC GGTAACCGGG 
CATGTACAAA CGGTCATTCA GGGCATGCCT ATACAGAATT CCCTGTCTGT GGAGAGCCTT 
AGCCCAGCAG TCAGTTTACA GACGTTGCAG CAGTCTGGCG GCGTCCCTAA TAATGGTAGC 
AGTGGATCGA CGGCAATGGC GCCATGCCAG CCAGGAGAGG GTATCACTGT ATTGGGCGGC 
TCTTCTGACT CTGCTGCTCA ACCAGCACAG ACGCAACCTC AGCCCTCAAT TCTCACGGTT 
CAGACGACGC CTCCAGTTTC AGTAGCAGCT TCGGTTCCTT CCTCTTCATC TCCATCCCCA 
ACGATGGCCA CGTCTGCATC CACAATGGTG GGTCTGTGTT CACAGGCCCA GCACAGCCCT 
GGAAAAGTGT TGTTCACGTC ACCTGGTTCC GGCATGATCC TGAGCCAGGA GCCTCTTCAA 
ATGTTCCTGC ACCAGGAGCA ACAGCATCAC GCAGGAAAAG ACCCACCTGC CGCTGTGGGT 
GTACCTGCAT CTGTTATCGT CAGCGGCAGC AGTTCTGGTC CCGTCCCTTC AGGCCATGAC 
AACTTGTTAG CTGAGACTCG GCAGAGGCAG AGTCCCAGTC CCTCCCTTGG TCCTGCCCAC 
ATGGCAACAG TGGTTAACAA GGTTCCATCG TCAGCACATT CACAGTCTAT AAAGATCCAA 
AACGCCTCCC AATCTCAGCC TGTGGTCACC CCTTCGCCAG CACCCACACT GTCCGACAGC 
CCTCAACCTG CTCAAGTTTC CCCTCTCACT CTCGGGCAGC AGATCCAGTC TCCCCATCAA 
CAGTCTCGGC CTCCCTCGCA GCCCCAGCCT CCTTCACAGG CACAGACTCC TTCACGCTCA 
TGCACCCCTT CCTCTCTACC GCCTCTCTTC ATCATTCACA ATCAGATGGG AGGTTCTCCA 
CAGCCGGCTC CACCACCTCA GCAACAGCAG CCTCAGCAGT TACAAGTGCA GCTCCAGCCT 
CAAGTTCTTC CTCAGCCTGC TGCTCTGCAG CCAGACGTAC CTCCTTCCTC GTGCTCCCCA 
AAGCCTCCGC AGCCACTTCC CACACAGTTC CAGTTCCAGC CCCCAGTCTC CAGCTCGTCT 
CCAGCTGCAG CAGTGAAACA GCAGGTGACG GTGGTGCCTG GGCTGACCGC AGAACAGCAG 
CATCACCTAC AGCTGGTCAG TGCGCAGCTG CAGACCATGT CATCAATCAC ACAGCCCTCC 
CCTCAACAAA AGCAGCTCTT GGAAAAACTT CACCAGGTCC AGCAAAACAT ACTTTTGCAG 
GCTAAGCAGC AAGCTCAGGC ACAAGCCCAG GCCTCTCAGC AACAGGCTAC AAACCAGTTC 
AACAAAATGC CAGATCAGCC TTCGGTCCAA GCTGCGACTT CTTCAACGGC CACTGGAGCC 
ATCCAAGCAC CAGTCCAGTC TCTTCTGCCG CAGAAGTCTG TGCTTGTCAA GCCCTCTACT 
ACAGGTGCAA ATGACACACA AGTGTTTTCT GCGGTGTCTG CTGGGGCTAC GGTGAACACG 
GGAATCACAA CACCAAACCT TGCACAGGCT GTCCAGGCAA AGCCAGGCGT CATAAGTTCG 
GTTAGTGGAC TAACTCTGGG CAAAGCAGGT TTGCAGATTC AGGTGTTAGG TGCTGGACTC 
TCTCAAATGC CTGCACCTCC ACTACCTGCT CCCCAAACAC AGACATCAAC ATTAAAAAGG 
CCCTTTAGTA TGGAACCAAG CAAAGAAGCT AGGATGCTGG AACAGCTGCG AAAACAGCAA 
GGCTCAGTTC TCCATCCTGA CTACAGCTCT CCTTTCCGCT CTTTTGAGGA CACACTTCAT 
CGGCTGCTGC CATACCATCT GTACCAAGGC ACGGCTTCAT CTCCGGAAGA CTGTCGTAAA 
GTGGATGATG AATTTGAGAA CGTCTCAAGC CAGCTCTTGA AACGCACACA AGCAATGCTG 
GATAAATATC GTCACTTGCT TTTCGAGGAA TCAAAGCAGA GGCTGGGTCC CTCAGCAGAG 
ATGGTTATGA TTGACCGGAT GTTCATTCAA GAGGAAAAGG TTGCTCTGAG TCAGGACAGG 
GTCCTAGCCA AGGAGAAACC AGAGGAATTT GTGGCCAATT CGTGTTTAGT GGACAGCAGT 
GCGGTGAAGC TTGAAAAGGT GGAGCTAAGT TCTGTAAGAA CTGCATCAAC AATGGTTGCG 
GCTCCGCCTA CAGCAGTTAC GCCTGCTCCT GCCACAACTG TTGCCCCGGC TCCAACCTCT 
ACACCTGCTC CAGCTGCTGC CCCAACTCCT GCTCCTGCTC CTGCCCCTGC ACTCTTCCCT 
CCAACAAAAC TGGTGATCAA GCAAGGTGGA GGAGGAGCTT CAGTCTCCTG GTCCACCAGC 
TCAACCCCTA CGCCTGCTCC GGTGGTGCGA CCGCCTGCTG AGCCTGTCGC ACCAAGTGCC 
TCCTTCAGCC GCACTCCGTC CTCCCGCCCT GCTGACGACG ACGATGACGT TGCACTTCCC 
CAACGGACCA GCAAACCACC CATCAAGACC TACGAGGCAC GTCGGCGGAT AGGTTTGAAG 
CTGAAGATCA AGCAAGAGGC GGGGTTAAGT AAGGTGGTTC ACAACACTGC ATTAGATCCT 
GTCCATTCGC AATCACAACT CACACCACAG ACGCCCACCC CCGAGCAGCC GCAGAAAGTA 
AAGCCACACG TAACTCTTCC CTCCACCGTC ATTAGAACTC AACCCACAAC CTCTCAATCC 
ACCTCCTCCT CCACTGTCAC CACAGTAACC ACACACACAG CCTCCGCCTC CAGTAGCGCA 
ACTTCCTCGA GCGGGGCATC GTCTTCATTC TCCACCTGGT CTTCATCGTC ACCTTCTACG 
TCGACCGCGC AGATGAACGG CACCCTGGAG CACCATGAGG TAGGTGGGGT CAAACGTAAC 
CCAGCGTCCA CAGCAACGCC ACCACTAACA ACGTGCAGGC TCCCGCTCCG AAAGACATAC 
CGTGAGAACA TCAGTCCCCG TCACAGGCCT GGAGTACCAG GAGGTGGTGG AGATACACTA 
CCCATTCTGC CTGCAGGACC TCCTGTTACC TCTTCGCCCC AACCGCAAAG CTCGTCCCCT 
CAACCCGAAC GAACTGTGAT AGCCAGTGTG AAACTGGAGC GGCAAGGTGG ACACGGGCGA 
TCTCACCCCC ATGCGGAGTC ACAAAGCCTG GCAGCCGTTG AAGACGTCCT TTATCGGGGA 
ATAAAAAACG CGTACCAACA TCATCGGGAG TTTGATAAAG ACGATGAGGA TGATATGGAG 
GAAGGTGGTG GTGGTGGTGG TGCTTTGGGG CGGTTAAAGG GCATAGGAAG CAAACACCGG 
GAAGGGGGAC GGGGCGCCTT CAGGATGGAT CAGCATGCCC CAGGGCCGCC CTCTCCCGGA 
GAGTCTTCCT GCACAAGAGA CTCGTCACTT CCTGCCAAAC GCTGCAAGTC GGACTCTCCG 
GACATGGACA ATGCAAGCTT CTCCAGCGGC AGCCCGCCGC CTGACGACTC GCTAAATGAG 
CACCTGCAGT GTGCCATAGA CAGTATACTA AACCTCCAAC AGGGCCCTCC TGGACACGGA 
GGCTCAGGGA GAGGGGATTT AGGGAGGGTT CATGGGGCAA ACCTGCCTAA CCAGCACCAA 
ACCCATCCCT CCTACAGACA GTCCATGCCT CCCCTCTCAA CACAGCCCTC GTCCGCCCCC 
ATGTCCCAGC ATTCACAGGT AGGAGGGCGA GGGCAGAATG GAAATCTGGT TTCACAGACG 
CACAGTAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bicra ( XM_005159253.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159310.1 Danio rerio glioma tumor suppressor candidate region gene 1 (gltscr1), transcript variant X2, mRNA.

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CloneID	ODa23559
Clone ID Related Accession (Same CDS sequence)	XM_005159253.3 , XM_005159253.4
Accession Version	XM_005159253.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	glioma tumor suppressor candidate region gene 1 protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159253.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATG AAGATGGCAG GTGCTTGCTA GATGTAATTT GTGACCCACA AGCACTCAAC 
GATTTTCTTC ATGGATCAGA AACACAACTG GACACTGATG ACCTATTGGA TGGCTCAGGT 
GACCCGTCCA GCTCGTTCTT CTCCAGCACT GGGGGCCATG TTCCTGAGGT TCCGGCACAG 
GTTCAGCTGT CGACAAGTGA GCCGGGCCTT CCAAGAGTCA GCGTTGATTT GGACTTCTTG 
GAGGACGATG ACATATTGGG GGGATCGCCA GGAGGAGACA ATGGAAGTAA TGGTGTTGGG 
ACAAATCATG AGCCCTGTGA CATTTTGCAG CAGAGCTTGG CGGAGGCTAA CATCACAGAG 
CAGAGCCTTC AAGAGGCAGA TGCTGAGCTT GACCTCAGTT CCTTTGGGCT AACTGGCCTT 
ACGCAGGTGG TTCAACCCCT GCCTGATGCC GGCCTGTCTG GGGTGGGCAT TGGAGGTGCA 
ACTCAGATTT TTCCAAACCA AGGCACCCCT ACCGCACCCT CAAATCCCAC TCCAGACATG 
CTTGGTTCTG TCCTGGCACA TCCAGGTCTG CAGCTTCAAC AACAGGTCAT GAACAAGGCT 
ATCAGCGTCC AGCCTTTTGT ACAACAAGTC GGACTTGGAA ATGTAACACT TCAGCCAATT 
TCGAGCCTTC AGGCCCTACC GAACGGCAGC CAGTCTGGAC CGTTGGGCAT TGGACAAATT 
CAAGTGGTGG GTCAGCCCAC TGTAATGACT ATAAATCCAT CTGGGCAGCA AATCTTGACG 
AAAACCATGG GTGGCTACCA ACTGCATCAA CCTGGGCCGG AGGCAGCAAG CGCTGGAACG 
CAGGCTGGGC TTGGAAGCTC TGTTTTGAGT TCAGGAGGTG GACTTGTAAT TCAAGGGGGC 
AAGGCCACTC TCGGGTCCCC TGCCTTAAAT GGGCCAGCTG TCTGCTTAGG CAGCACAAAT 
ACCAACAACA GTGCAACTAC AATGGCCACT GCTGGTGGTA TAGTGGGTTT TAGTAATGCC 
TCTTTGGGTG CAGGAATTGG ATCTCAGACC CAGCCTCAAG GCCAAATCAT GCAGAATGTC 
ATCATCCAGC GAACGCCAAC GCCAATACAG CCCAAACCTC CTCAAGGGGG TACAATCCAA 
CCCAAAATCT TCAAGCAACA ACAACAACAG CTTCCTGCCC CACATGCCTT GCCAAATGAT 
GCTAATAAAG CTCTGGGGGT ACAGCAAATC CCAGTGTCAG CTGGTCAGAA TGTAACGTTC 
CTCACCGGGA AGCCTGGCTC TAACGTAGTT TTGAGCACAC AGGCAGCGTC GCAGGGAACA 
CAGTTTTCTC AAGCGCTCTT TAAACAGCAG GGCCCTCAAA CTTCAGGCAA ACCCCTCAGT 
GTTCACTTGT TAAACCAACA GGGCAGCATT GTCATTCCCT CGCAAACTGT CCTGCAGGGC 
CAAAACCACC AGTTCCTCTT ACCAGGGCTT CAAGCAGGAG GCCAGATCCT GGCTCAGCAT 
CCTGGTGGAC ACATCATAAC CAGTCAGGGG CCTGGAGGGC AGATAATCGC TAATCAGATC 
TTGACAGCCA ATCAGAACAT CAACTTGGGA CAGGTGTTGG CTTCACAGGG CCACCCTGGT 
GCAGCCCACA TTCTCTCTGG ACACATCCAG CTCCAGCCTG GTCAGATGGG TCAGCCTGCA 
CTGTTCCAAA TGCCGGTGTC TTTGGCGCAT ACTCAGACAC AATCTCATCC GGTAACCGGG 
CATGTACAAA CGGTCATTCA GGGCATGCCT ATACAGAATT CCCTGTCTGT GGAGAGCCTT 
AGCCCAGCAG TCAGTTTACA GACGTTGCAG CAGTCTGGCG GCGTCCCTAA TAATGGTAGC 
AGTGGATCGA CGGCAATGGC GCCATGCCAG CCAGGAGAGG GTATCACTGT ATTGGGCGGC 
TCTTCTGACT CTGCTGCTCA ACCAGCACAG ACGCAACCTC AGCCCTCAAT TCTCACGGTT 
CAGACGACGC CTCCAGTTTC AGTAGCAGCT TCGGTTCCTT CCTCTTCATC TCCATCCCCA 
ACGATGGCCA CGTCTGCATC CACAATGGTG GGTCTGTGTT CACAGGCCCA GCACAGCCCT 
GGAAAAGTGT TGTTCACGTC ACCTGGTTCC GGCATGATCC TGAGCCAGGA GCCTCTTCAA 
ATGTTCCTGC ACCAGGAGCA ACAGCATCAC GCAGGAAAAG ACCCACCTGC CGCTGTGGGT 
GTACCTGCAT CTGTTATCGT CAGCGGCAGC AGTTCTGGTC CCGTCCCTTC AGGCCATGAC 
AACTTGTTAG CTGAGACTCG GCAGAGGCAG AGTCCCAGTC CCTCCCTTGG TCCTGCCCAC 
ATGGCAACAG TGGTTAACAA GGTTCCATCG TCAGCACATT CACAGTCTAT AAAGATCCAA 
AACGCCTCCC AATCTCAGCC TGTGGTCACC CCTTCGCCAG CACCCACACT GTCCGACAGC 
CCTCAACCTG CTCAAGTTTC CCCTCTCACT CTCGGGCAGC AGATCCAGTC TCCCCATCAA 
CAGTCTCGGC CTCCCTCGCA GCCCCAGCCT CCTTCACAGG CACAGACTCC TTCACGCTCA 
TGCACCCCTT CCTCTCTACC GCCTCTCTTC ATCATTCACA ATCAGATGGG AGGTTCTCCA 
CAGCCGGCTC CACCACCTCA GCAACAGCAG CCTCAGCAGT TACAAGTGCA GCTCCAGCCT 
CAAGTTCTTC CTCAGCCTGC TGCTCTGCAG CCAGACGTAC CTCCTTCCTC GTGCTCCCCA 
AAGCCTCCGC AGCCACTTCC CACACAGTTC CAGTTCCAGC CCCCAGTCTC CAGCTCGTCT 
CCAGCTGCAG CAGTGAAACA GCAGGTGACG GTGGTGCCTG GGCTGACCGC AGAACAGCAG 
CATCACCTAC AGCTGGTCAG TGCGCAGCTG CAGACCATGT CATCAATCAC ACAGCCCTCC 
CCTCAACAAA AGCAGCTCTT GGAAAAACTT CACCAGGTCC AGCAAAACAT ACTTTTGCAG 
GCTAAGCAGC AAGCTCAGGC ACAAGCCCAG GCCTCTCAGC AACAGGCTAC AAACCAGTTC 
AACAAAATGC CAGATCAGCC TTCGGTCCAA GCTGCGACTT CTTCAACGGC CACTGGAGCC 
ATCCAAGCAC CAGTCCAGTC TCTTCTGCCG CAGAAGTCTG TGCTTGTCAA GCCCTCTACT 
ACAGGTGCAA ATGACACACA AGTGTTTTCT GCGGTGTCTG CTGGGGCTAC GGTGAACACG 
GGAATCACAA CACCAAACCT TGCACAGGCT GTCCAGGCAA AGCCAGGCGT CATAAGTTCG 
GTTAGTGGAC TAACTCTGGG CAAAGCAGGT TTGCAGATTC AGGTGTTAGG TGCTGGACTC 
TCTCAAATGC CTGCACCTCC ACTACCTGCT CCCCAAACAC AGACATCAAC ATTAAAAAGG 
CCCTTTAGTA TGGAACCAAG CAAAGAAGCT AGGATGCTGG AACAGCTGCG AAAACAGCAA 
GGCTCAGTTC TCCATCCTGA CTACAGCTCT CCTTTCCGCT CTTTTGAGGA CACACTTCAT 
CGGCTGCTGC CATACCATCT GTACCAAGGC ACGGCTTCAT CTCCGGAAGA CTGTCGTAAA 
GTGGATGATG AATTTGAGAA CGTCTCAAGC CAGCTCTTGA AACGCACACA AGCAATGCTG 
GATAAATATC GTCACTTGCT TTTCGAGGAA TCAAAGAGGC TGGGTCCCTC AGCAGAGATG 
GTTATGATTG ACCGGATGTT CATTCAAGAG GAAAAGGTTG CTCTGAGTCA GGACAGGGTC 
CTAGCCAAGG AGAAACCAGA GGAATTTGTG GCCAATTCGT GTTTAGTGGA CAGCAGTGCG 
GTGAAGCTTG AAAAGGTGGA GCTAAGTTCT GTAAGAACTG CATCAACAAT GGTTGCGGCT 
CCGCCTACAG CAGTTACGCC TGCTCCTGCC ACAACTGTTG CCCCGGCTCC AACCTCTACA 
CCTGCTCCAG CTGCTGCCCC AACTCCTGCT CCTGCTCCTG CCCCTGCACT CTTCCCTCCA 
ACAAAACTGG TGATCAAGCA AGGTGGAGGA GGAGCTTCAG TCTCCTGGTC CACCAGCTCA 
ACCCCTACGC CTGCTCCGGT GGTGCGACCG CCTGCTGAGC CTGTCGCACC AAGTGCCTCC 
TTCAGCCGCA CTCCGTCCTC CCGCCCTGCT GACGACGACG ATGACGTTGC ACTTCCCCAA 
CGGACCAGCA AACCACCCAT CAAGACCTAC GAGGCACGTC GGCGGATAGG TTTGAAGCTG 
AAGATCAAGC AAGAGGCGGG GTTAAGTAAG GTGGTTCACA ACACTGCATT AGATCCTGTC 
CATTCGCAAT CACAACTCAC ACCACAGACG CCCACCCCCG AGCAGCCGCA GAAAGTAAAG 
CCACACGTAA CTCTTCCCTC CACCGTCATT AGAACTCAAC CCACAACCTC TCAATCCACC 
TCCTCCTCCA CTGTCACCAC AGTAACCACA CACACAGCCT CCGCCTCCAG TAGCGCAACT 
TCCTCGAGCG GGGCATCGTC TTCATTCTCC ACCTGGTCTT CATCGTCACC TTCTACGTCG 
ACCGCGCAGA TGAACGGCAC CCTGGAGCAC CATGAGGTAG GTGGGGTCAA ACGTAACCCA 
GCGTCCACAG CAACGCCACC ACTAACAACG TGCAGGCTCC CGCTCCGAAA GACATACCGT 
GAGAACATCA GTCCCCGTCA CAGGCCTGGA GTACCAGGAG GTGGTGGAGA TACACTACCC 
ATTCTGCCTG CAGGACCTCC TGTTACCTCT TCGCCCCAAC CGCAAAGCTC GTCCCCTCAA 
CCCGAACGAA CTGTGATAGC CAGTGTGAAA CTGGAGCGGC AAGGTGGACA CGGGCGATCT 
CACCCCCATG CGGAGTCACA AAGCCTGGCA GCCGTTGAAG ACGTCCTTTA TCGGGGAATA 
AAAAACGCGT ACCAACATCA TCGGGAGTTT GATAAAGACG ATGAGGATGA TATGGAGGAA 
GGTGGTGGTG GTGGTGGTGC TTTGGGGCGG TTAAAGGGCA TAGGAAGCAA ACACCGGGAA 
GGGGGACGGG GCGCCTTCAG GATGGATCAG CATGCCCCAG GGCCGCCCTC TCCCGGAGAG 
TCTTCCTGCA CAAGAGACTC GTCACTTCCT GCCAAACGCT GCAAGTCGGA CTCTCCGGAC 
ATGGACAATG CAAGCTTCTC CAGCGGCAGC CCGCCGCCTG ACGACTCGCT AAATGAGCAC 
CTGCAGTGTG CCATAGACAG TATACTAAAC CTCCAACAGG GCCCTCCTGG ACACGGAGGC 
TCAGGGAGAG GGGATTTAGG GAGGGTTCAT GGGGCAAACC TGCCTAACCA GCACCAAACC 
CATCCCTCCT ACAGACAGTC CATGCCTCCC CTCTCAACAC AGCCCTCGTC CGCCCCCATG 
TCCCAGCATT CACAGGTAGG AGGGCGAGGG CAGAATGGAA ATCTGGTTTC ACAGACGCAC 
AGTAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159310.1
CDS	1149..6617
Translation	MDDEDGRCLLDVICDPQALNDFLHGSETQLDTDDLLDGSGDPSSSFFSSTGGHVPEVPAQVQLSTSEPGLPRVSVDLDFLEDDDILGGSPGGDNGSNGVGTNHEPCDILQQSLAEANITEQSLQEADAELDLSSFGLTGLTQVVQPLPDAGLSGVGIGGATQIFPNQGTPTAPSNPTPDMLGSVLAHPGLQLQQQVMNKAISVQPFVQQVGLGNVTLQPISSLQALPNGSQSGPLGIGQIQVVGQPTVMTINPSGQQILTKTMGGYQLHQPGPEAASAGTQAGLGSSVLSSGGGLVIQGGKATLGSPALNGPAVCLGSTNTNNSATTMATAGGIVGFSNASLGAGIGSQTQPQGQIMQNVIIQRTPTPIQPKPPQGGTIQPKIFKQQQQQLPAPHALPNDANKALGVQQIPVSAGQNVTFLTGKPGSNVVLSTQAASQGTQFSQALFKQQGPQTSGKPLSVHLLNQQGSIVIPSQTVLQGQNHQFLLPGLQAGGQILAQHPGGHIITSQGPGGQIIANQILTANQNINLGQVLASQGHPGAAHILSGHIQLQPGQMGQPALFQMPVSLAHTQTQSHPVTGHVQTVIQGMPIQNSLSVESLSPAVSLQTLQQSGGVPNNGSSGSTAMAPCQPGEGITVLGGSSDSAAQPAQTQPQPSILTVQTTPPVSVAASVPSSSSPSPTMATSASTMVGLCSQAQHSPGKVLFTSPGSGMILSQEPLQMFLHQEQQHHAGKDPPAAVGVPASVIVSGSSSGPVPSGHDNLLAETRQRQSPSPSLGPAHMATVVNKVPSSAHSQSIKIQNASQSQPVVTPSPAPTLSDSPQPAQVSPLTLGQQIQSPHQQSRPPSQPQPPSQAQTPSRSCTPSSLPPLFIIHNQMGGSPQPAPPPQQQQPQQLQVQLQPQVLPQPAALQPDVPPSSCSPKPPQPLPTQFQFQPPVSSSSPAAAVKQQVTVVPGLTAEQQHHLQLVSAQLQTMSSITQPSPQQKQLLEKLHQVQQNILLQAKQQAQAQAQASQQQATNQFNKMPDQPSVQAATSSTATGAIQAPVQSLLPQKSVLVKPSTTGANDTQVFSAVSAGATVNTGITTPNLAQAVQAKPGVISSVSGLTLGKAGLQIQVLGAGLSQMPAPPLPAPQTQTSTLKRPFSMEPSKEARMLEQLRKQQGSVLHPDYSSPFRSFEDTLHRLLPYHLYQGTASSPEDCRKVDDEFENVSSQLLKRTQAMLDKYRHLLFEESKRLGPSAEMVMIDRMFIQEEKVALSQDRVLAKEKPEEFVANSCLVDSSAVKLEKVELSSVRTASTMVAAPPTAVTPAPATTVAPAPTSTPAPAAAPTPAPAPAPALFPPTKLVIKQGGGGASVSWSTSSTPTPAPVVRPPAEPVAPSASFSRTPSSRPADDDDDVALPQRTSKPPIKTYEARRRIGLKLKIKQEAGLSKVVHNTALDPVHSQSQLTPQTPTPEQPQKVKPHVTLPSTVIRTQPTTSQSTSSSTVTTVTTHTASASSSATSSSGASSSFSTWSSSSPSTSTAQMNGTLEHHEVGGVKRNPASTATPPLTTCRLPLRKTYRENISPRHRPGVPGGGGDTLPILPAGPPVTSSPQPQSSSPQPERTVIASVKLERQGGHGRSHPHAESQSLAAVEDVLYRGIKNAYQHHREFDKDDEDDMEEGGGGGGALGRLKGIGSKHREGGRGAFRMDQHAPGPPSPGESSCTRDSSLPAKRCKSDSPDMDNASFSSGSPPPDDSLNEHLQCAIDSILNLQQGPPGHGGSGRGDLGRVHGANLPNQHQTHPSYRQSMPPLSTQPSSAPMSQHSQVGGRGQNGNLVSQTHSR

Target ORF information:

RefSeq Version	XM_005159253.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio glioma tumor suppressor candidate region gene 1 (gltscr1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159253.3

ORF Insert Sequence:

ATGGATGATG AAGATGGCAG GTGCTTGCTA GATGTAATTT GTGACCCACA AGCACTCAAC 
GATTTTCTTC ATGGATCAGA AACACAACTG GACACTGATG ACCTATTGGA TGGCTCAGGT 
GACCCGTCCA GCTCGTTCTT CTCCAGCACT GGGGGCCATG TTCCTGAGGT TCCGGCACAG 
GTTCAGCTGT CGACAAGTGA GCCGGGCCTT CCAAGAGTCA GCGTTGATTT GGACTTCTTG 
GAGGACGATG ACATATTGGG GGGATCGCCA GGAGGAGACA ATGGAAGTAA TGGTGTTGGG 
ACAAATCATG AGCCCTGTGA CATTTTGCAG CAGAGCTTGG CGGAGGCTAA CATCACAGAG 
CAGAGCCTTC AAGAGGCAGA TGCTGAGCTT GACCTCAGTT CCTTTGGGCT AACTGGCCTT 
ACGCAGGTGG TTCAACCCCT GCCTGATGCC GGCCTGTCTG GGGTGGGCAT TGGAGGTGCA 
ACTCAGATTT TTCCAAACCA AGGCACCCCT ACCGCACCCT CAAATCCCAC TCCAGACATG 
CTTGGTTCTG TCCTGGCACA TCCAGGTCTG CAGCTTCAAC AACAGGTCAT GAACAAGGCT 
ATCAGCGTCC AGCCTTTTGT ACAACAAGTC GGACTTGGAA ATGTAACACT TCAGCCAATT 
TCGAGCCTTC AGGCCCTACC GAACGGCAGC CAGTCTGGAC CGTTGGGCAT TGGACAAATT 
CAAGTGGTGG GTCAGCCCAC TGTAATGACT ATAAATCCAT CTGGGCAGCA AATCTTGACG 
AAAACCATGG GTGGCTACCA ACTGCATCAA CCTGGGCCGG AGGCAGCAAG CGCTGGAACG 
CAGGCTGGGC TTGGAAGCTC TGTTTTGAGT TCAGGAGGTG GACTTGTAAT TCAAGGGGGC 
AAGGCCACTC TCGGGTCCCC TGCCTTAAAT GGGCCAGCTG TCTGCTTAGG CAGCACAAAT 
ACCAACAACA GTGCAACTAC AATGGCCACT GCTGGTGGTA TAGTGGGTTT TAGTAATGCC 
TCTTTGGGTG CAGGAATTGG ATCTCAGACC CAGCCTCAAG GCCAAATCAT GCAGAATGTC 
ATCATCCAGC GAACGCCAAC GCCAATACAG CCCAAACCTC CTCAAGGGGG TACAATCCAA 
CCCAAAATCT TCAAGCAACA ACAACAACAG CTTCCTGCCC CACATGCCTT GCCAAATGAT 
GCTAATAAAG CTCTGGGGGT ACAGCAAATC CCAGTGTCAG CTGGTCAGAA TGTAACGTTC 
CTCACCGGGA AGCCTGGCTC TAACGTAGTT TTGAGCACAC AGGCAGCGTC GCAGGGAACA 
CAGTTTTCTC AAGCGCTCTT TAAACAGCAG GGCCCTCAAA CTTCAGGCAA ACCCCTCAGT 
GTTCACTTGT TAAACCAACA GGGCAGCATT GTCATTCCCT CGCAAACTGT CCTGCAGGGC 
CAAAACCACC AGTTCCTCTT ACCAGGGCTT CAAGCAGGAG GCCAGATCCT GGCTCAGCAT 
CCTGGTGGAC ACATCATAAC CAGTCAGGGG CCTGGAGGGC AGATAATCGC TAATCAGATC 
TTGACAGCCA ATCAGAACAT CAACTTGGGA CAGGTGTTGG CTTCACAGGG CCACCCTGGT 
GCAGCCCACA TTCTCTCTGG ACACATCCAG CTCCAGCCTG GTCAGATGGG TCAGCCTGCA 
CTGTTCCAAA TGCCGGTGTC TTTGGCGCAT ACTCAGACAC AATCTCATCC GGTAACCGGG 
CATGTACAAA CGGTCATTCA GGGCATGCCT ATACAGAATT CCCTGTCTGT GGAGAGCCTT 
AGCCCAGCAG TCAGTTTACA GACGTTGCAG CAGTCTGGCG GCGTCCCTAA TAATGGTAGC 
AGTGGATCGA CGGCAATGGC GCCATGCCAG CCAGGAGAGG GTATCACTGT ATTGGGCGGC 
TCTTCTGACT CTGCTGCTCA ACCAGCACAG ACGCAACCTC AGCCCTCAAT TCTCACGGTT 
CAGACGACGC CTCCAGTTTC AGTAGCAGCT TCGGTTCCTT CCTCTTCATC TCCATCCCCA 
ACGATGGCCA CGTCTGCATC CACAATGGTG GGTCTGTGTT CACAGGCCCA GCACAGCCCT 
GGAAAAGTGT TGTTCACGTC ACCTGGTTCC GGCATGATCC TGAGCCAGGA GCCTCTTCAA 
ATGTTCCTGC ACCAGGAGCA ACAGCATCAC GCAGGAAAAG ACCCACCTGC CGCTGTGGGT 
GTACCTGCAT CTGTTATCGT CAGCGGCAGC AGTTCTGGTC CCGTCCCTTC AGGCCATGAC 
AACTTGTTAG CTGAGACTCG GCAGAGGCAG AGTCCCAGTC CCTCCCTTGG TCCTGCCCAC 
ATGGCAACAG TGGTTAACAA GGTTCCATCG TCAGCACATT CACAGTCTAT AAAGATCCAA 
AACGCCTCCC AATCTCAGCC TGTGGTCACC CCTTCGCCAG CACCCACACT GTCCGACAGC 
CCTCAACCTG CTCAAGTTTC CCCTCTCACT CTCGGGCAGC AGATCCAGTC TCCCCATCAA 
CAGTCTCGGC CTCCCTCGCA GCCCCAGCCT CCTTCACAGG CACAGACTCC TTCACGCTCA 
TGCACCCCTT CCTCTCTACC GCCTCTCTTC ATCATTCACA ATCAGATGGG AGGTTCTCCA 
CAGCCGGCTC CACCACCTCA GCAACAGCAG CCTCAGCAGT TACAAGTGCA GCTCCAGCCT 
CAAGTTCTTC CTCAGCCTGC TGCTCTGCAG CCAGACGTAC CTCCTTCCTC GTGCTCCCCA 
AAGCCTCCGC AGCCACTTCC CACACAGTTC CAGTTCCAGC CCCCAGTCTC CAGCTCGTCT 
CCAGCTGCAG CAGTGAAACA GCAGGTGACG GTGGTGCCTG GGCTGACCGC AGAACAGCAG 
CATCACCTAC AGCTGGTCAG TGCGCAGCTG CAGACCATGT CATCAATCAC ACAGCCCTCC 
CCTCAACAAA AGCAGCTCTT GGAAAAACTT CACCAGGTCC AGCAAAACAT ACTTTTGCAG 
GCTAAGCAGC AAGCTCAGGC ACAAGCCCAG GCCTCTCAGC AACAGGCTAC AAACCAGTTC 
AACAAAATGC CAGATCAGCC TTCGGTCCAA GCTGCGACTT CTTCAACGGC CACTGGAGCC 
ATCCAAGCAC CAGTCCAGTC TCTTCTGCCG CAGAAGTCTG TGCTTGTCAA GCCCTCTACT 
ACAGGTGCAA ATGACACACA AGTGTTTTCT GCGGTGTCTG CTGGGGCTAC GGTGAACACG 
GGAATCACAA CACCAAACCT TGCACAGGCT GTCCAGGCAA AGCCAGGCGT CATAAGTTCG 
GTTAGTGGAC TAACTCTGGG CAAAGCAGGT TTGCAGATTC AGGTGTTAGG TGCTGGACTC 
TCTCAAATGC CTGCACCTCC ACTACCTGCT CCCCAAACAC AGACATCAAC ATTAAAAAGG 
CCCTTTAGTA TGGAACCAAG CAAAGAAGCT AGGATGCTGG AACAGCTGCG AAAACAGCAA 
GGCTCAGTTC TCCATCCTGA CTACAGCTCT CCTTTCCGCT CTTTTGAGGA CACACTTCAT 
CGGCTGCTGC CATACCATCT GTACCAAGGC ACGGCTTCAT CTCCGGAAGA CTGTCGTAAA 
GTGGATGATG AATTTGAGAA CGTCTCAAGC CAGCTCTTGA AACGCACACA AGCAATGCTG 
GATAAATATC GTCACTTGCT TTTCGAGGAA TCAAAGAGGC TGGGTCCCTC AGCAGAGATG 
GTTATGATTG ACCGGATGTT CATTCAAGAG GAAAAGGTTG CTCTGAGTCA GGACAGGGTC 
CTAGCCAAGG AGAAACCAGA GGAATTTGTG GCCAATTCGT GTTTAGTGGA CAGCAGTGCG 
GTGAAGCTTG AAAAGGTGGA GCTAAGTTCT GTAAGAACTG CATCAACAAT GGTTGCGGCT 
CCGCCTACAG CAGTTACGCC TGCTCCTGCC ACAACTGTTG CCCCGGCTCC AACCTCTACA 
CCTGCTCCAG CTGCTGCCCC AACTCCTGCT CCTGCTCCTG CCCCTGCACT CTTCCCTCCA 
ACAAAACTGG TGATCAAGCA AGGTGGAGGA GGAGCTTCAG TCTCCTGGTC CACCAGCTCA 
ACCCCTACGC CTGCTCCGGT GGTGCGACCG CCTGCTGAGC CTGTCGCACC AAGTGCCTCC 
TTCAGCCGCA CTCCGTCCTC CCGCCCTGCT GACGACGACG ATGACGTTGC ACTTCCCCAA 
CGGACCAGCA AACCACCCAT CAAGACCTAC GAGGCACGTC GGCGGATAGG TTTGAAGCTG 
AAGATCAAGC AAGAGGCGGG GTTAAGTAAG GTGGTTCACA ACACTGCATT AGATCCTGTC 
CATTCGCAAT CACAACTCAC ACCACAGACG CCCACCCCCG AGCAGCCGCA GAAAGTAAAG 
CCACACGTAA CTCTTCCCTC CACCGTCATT AGAACTCAAC CCACAACCTC TCAATCCACC 
TCCTCCTCCA CTGTCACCAC AGTAACCACA CACACAGCCT CCGCCTCCAG TAGCGCAACT 
TCCTCGAGCG GGGCATCGTC TTCATTCTCC ACCTGGTCTT CATCGTCACC TTCTACGTCG 
ACCGCGCAGA TGAACGGCAC CCTGGAGCAC CATGAGGTAG GTGGGGTCAA ACGTAACCCA 
GCGTCCACAG CAACGCCACC ACTAACAACG TGCAGGCTCC CGCTCCGAAA GACATACCGT 
GAGAACATCA GTCCCCGTCA CAGGCCTGGA GTACCAGGAG GTGGTGGAGA TACACTACCC 
ATTCTGCCTG CAGGACCTCC TGTTACCTCT TCGCCCCAAC CGCAAAGCTC GTCCCCTCAA 
CCCGAACGAA CTGTGATAGC CAGTGTGAAA CTGGAGCGGC AAGGTGGACA CGGGCGATCT 
CACCCCCATG CGGAGTCACA AAGCCTGGCA GCCGTTGAAG ACGTCCTTTA TCGGGGAATA 
AAAAACGCGT ACCAACATCA TCGGGAGTTT GATAAAGACG ATGAGGATGA TATGGAGGAA 
GGTGGTGGTG GTGGTGGTGC TTTGGGGCGG TTAAAGGGCA TAGGAAGCAA ACACCGGGAA 
GGGGGACGGG GCGCCTTCAG GATGGATCAG CATGCCCCAG GGCCGCCCTC TCCCGGAGAG 
TCTTCCTGCA CAAGAGACTC GTCACTTCCT GCCAAACGCT GCAAGTCGGA CTCTCCGGAC 
ATGGACAATG CAAGCTTCTC CAGCGGCAGC CCGCCGCCTG ACGACTCGCT AAATGAGCAC 
CTGCAGTGTG CCATAGACAG TATACTAAAC CTCCAACAGG GCCCTCCTGG ACACGGAGGC 
TCAGGGAGAG GGGATTTAGG GAGGGTTCAT GGGGCAAACC TGCCTAACCA GCACCAAACC 
CATCCCTCCT ACAGACAGTC CATGCCTCCC CTCTCAACAC AGCCCTCGTC CGCCCCCATG 
TCCCAGCATT CACAGGTAGG AGGGCGAGGG CAGAATGGAA ATCTGGTTTC ACAGACGCAC 
AGTAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bicra ( XM_005159253.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159310.1 Danio rerio BRD4 interacting chromatin remodeling complex associated protein (bicra), transcript variant X2, mRNA.

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CloneID	ODa23559
Clone ID Related Accession (Same CDS sequence)	XM_005159253.3 , XM_005159253.4
Accession Version	XM_005159253.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	glioma tumor suppressor candidate region gene 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159253.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATG AAGATGGCAG GTGCTTGCTA GATGTAATTT GTGACCCACA AGCACTCAAC 
GATTTTCTTC ATGGATCAGA AACACAACTG GACACTGATG ACCTATTGGA TGGCTCAGGT 
GACCCGTCCA GCTCGTTCTT CTCCAGCACT GGGGGCCATG TTCCTGAGGT TCCGGCACAG 
GTTCAGCTGT CGACAAGTGA GCCGGGCCTT CCAAGAGTCA GCGTTGATTT GGACTTCTTG 
GAGGACGATG ACATATTGGG GGGATCGCCA GGAGGAGACA ATGGAAGTAA TGGTGTTGGG 
ACAAATCATG AGCCCTGTGA CATTTTGCAG CAGAGCTTGG CGGAGGCTAA CATCACAGAG 
CAGAGCCTTC AAGAGGCAGA TGCTGAGCTT GACCTCAGTT CCTTTGGGCT AACTGGCCTT 
ACGCAGGTGG TTCAACCCCT GCCTGATGCC GGCCTGTCTG GGGTGGGCAT TGGAGGTGCA 
ACTCAGATTT TTCCAAACCA AGGCACCCCT ACCGCACCCT CAAATCCCAC TCCAGACATG 
CTTGGTTCTG TCCTGGCACA TCCAGGTCTG CAGCTTCAAC AACAGGTCAT GAACAAGGCT 
ATCAGCGTCC AGCCTTTTGT ACAACAAGTC GGACTTGGAA ATGTAACACT TCAGCCAATT 
TCGAGCCTTC AGGCCCTACC GAACGGCAGC CAGTCTGGAC CGTTGGGCAT TGGACAAATT 
CAAGTGGTGG GTCAGCCCAC TGTAATGACT ATAAATCCAT CTGGGCAGCA AATCTTGACG 
AAAACCATGG GTGGCTACCA ACTGCATCAA CCTGGGCCGG AGGCAGCAAG CGCTGGAACG 
CAGGCTGGGC TTGGAAGCTC TGTTTTGAGT TCAGGAGGTG GACTTGTAAT TCAAGGGGGC 
AAGGCCACTC TCGGGTCCCC TGCCTTAAAT GGGCCAGCTG TCTGCTTAGG CAGCACAAAT 
ACCAACAACA GTGCAACTAC AATGGCCACT GCTGGTGGTA TAGTGGGTTT TAGTAATGCC 
TCTTTGGGTG CAGGAATTGG ATCTCAGACC CAGCCTCAAG GCCAAATCAT GCAGAATGTC 
ATCATCCAGC GAACGCCAAC GCCAATACAG CCCAAACCTC CTCAAGGGGG TACAATCCAA 
CCCAAAATCT TCAAGCAACA ACAACAACAG CTTCCTGCCC CACATGCCTT GCCAAATGAT 
GCTAATAAAG CTCTGGGGGT ACAGCAAATC CCAGTGTCAG CTGGTCAGAA TGTAACGTTC 
CTCACCGGGA AGCCTGGCTC TAACGTAGTT TTGAGCACAC AGGCAGCGTC GCAGGGAACA 
CAGTTTTCTC AAGCGCTCTT TAAACAGCAG GGCCCTCAAA CTTCAGGCAA ACCCCTCAGT 
GTTCACTTGT TAAACCAACA GGGCAGCATT GTCATTCCCT CGCAAACTGT CCTGCAGGGC 
CAAAACCACC AGTTCCTCTT ACCAGGGCTT CAAGCAGGAG GCCAGATCCT GGCTCAGCAT 
CCTGGTGGAC ACATCATAAC CAGTCAGGGG CCTGGAGGGC AGATAATCGC TAATCAGATC 
TTGACAGCCA ATCAGAACAT CAACTTGGGA CAGGTGTTGG CTTCACAGGG CCACCCTGGT 
GCAGCCCACA TTCTCTCTGG ACACATCCAG CTCCAGCCTG GTCAGATGGG TCAGCCTGCA 
CTGTTCCAAA TGCCGGTGTC TTTGGCGCAT ACTCAGACAC AATCTCATCC GGTAACCGGG 
CATGTACAAA CGGTCATTCA GGGCATGCCT ATACAGAATT CCCTGTCTGT GGAGAGCCTT 
AGCCCAGCAG TCAGTTTACA GACGTTGCAG CAGTCTGGCG GCGTCCCTAA TAATGGTAGC 
AGTGGATCGA CGGCAATGGC GCCATGCCAG CCAGGAGAGG GTATCACTGT ATTGGGCGGC 
TCTTCTGACT CTGCTGCTCA ACCAGCACAG ACGCAACCTC AGCCCTCAAT TCTCACGGTT 
CAGACGACGC CTCCAGTTTC AGTAGCAGCT TCGGTTCCTT CCTCTTCATC TCCATCCCCA 
ACGATGGCCA CGTCTGCATC CACAATGGTG GGTCTGTGTT CACAGGCCCA GCACAGCCCT 
GGAAAAGTGT TGTTCACGTC ACCTGGTTCC GGCATGATCC TGAGCCAGGA GCCTCTTCAA 
ATGTTCCTGC ACCAGGAGCA ACAGCATCAC GCAGGAAAAG ACCCACCTGC CGCTGTGGGT 
GTACCTGCAT CTGTTATCGT CAGCGGCAGC AGTTCTGGTC CCGTCCCTTC AGGCCATGAC 
AACTTGTTAG CTGAGACTCG GCAGAGGCAG AGTCCCAGTC CCTCCCTTGG TCCTGCCCAC 
ATGGCAACAG TGGTTAACAA GGTTCCATCG TCAGCACATT CACAGTCTAT AAAGATCCAA 
AACGCCTCCC AATCTCAGCC TGTGGTCACC CCTTCGCCAG CACCCACACT GTCCGACAGC 
CCTCAACCTG CTCAAGTTTC CCCTCTCACT CTCGGGCAGC AGATCCAGTC TCCCCATCAA 
CAGTCTCGGC CTCCCTCGCA GCCCCAGCCT CCTTCACAGG CACAGACTCC TTCACGCTCA 
TGCACCCCTT CCTCTCTACC GCCTCTCTTC ATCATTCACA ATCAGATGGG AGGTTCTCCA 
CAGCCGGCTC CACCACCTCA GCAACAGCAG CCTCAGCAGT TACAAGTGCA GCTCCAGCCT 
CAAGTTCTTC CTCAGCCTGC TGCTCTGCAG CCAGACGTAC CTCCTTCCTC GTGCTCCCCA 
AAGCCTCCGC AGCCACTTCC CACACAGTTC CAGTTCCAGC CCCCAGTCTC CAGCTCGTCT 
CCAGCTGCAG CAGTGAAACA GCAGGTGACG GTGGTGCCTG GGCTGACCGC AGAACAGCAG 
CATCACCTAC AGCTGGTCAG TGCGCAGCTG CAGACCATGT CATCAATCAC ACAGCCCTCC 
CCTCAACAAA AGCAGCTCTT GGAAAAACTT CACCAGGTCC AGCAAAACAT ACTTTTGCAG 
GCTAAGCAGC AAGCTCAGGC ACAAGCCCAG GCCTCTCAGC AACAGGCTAC AAACCAGTTC 
AACAAAATGC CAGATCAGCC TTCGGTCCAA GCTGCGACTT CTTCAACGGC CACTGGAGCC 
ATCCAAGCAC CAGTCCAGTC TCTTCTGCCG CAGAAGTCTG TGCTTGTCAA GCCCTCTACT 
ACAGGTGCAA ATGACACACA AGTGTTTTCT GCGGTGTCTG CTGGGGCTAC GGTGAACACG 
GGAATCACAA CACCAAACCT TGCACAGGCT GTCCAGGCAA AGCCAGGCGT CATAAGTTCG 
GTTAGTGGAC TAACTCTGGG CAAAGCAGGT TTGCAGATTC AGGTGTTAGG TGCTGGACTC 
TCTCAAATGC CTGCACCTCC ACTACCTGCT CCCCAAACAC AGACATCAAC ATTAAAAAGG 
CCCTTTAGTA TGGAACCAAG CAAAGAAGCT AGGATGCTGG AACAGCTGCG AAAACAGCAA 
GGCTCAGTTC TCCATCCTGA CTACAGCTCT CCTTTCCGCT CTTTTGAGGA CACACTTCAT 
CGGCTGCTGC CATACCATCT GTACCAAGGC ACGGCTTCAT CTCCGGAAGA CTGTCGTAAA 
GTGGATGATG AATTTGAGAA CGTCTCAAGC CAGCTCTTGA AACGCACACA AGCAATGCTG 
GATAAATATC GTCACTTGCT TTTCGAGGAA TCAAAGAGGC TGGGTCCCTC AGCAGAGATG 
GTTATGATTG ACCGGATGTT CATTCAAGAG GAAAAGGTTG CTCTGAGTCA GGACAGGGTC 
CTAGCCAAGG AGAAACCAGA GGAATTTGTG GCCAATTCGT GTTTAGTGGA CAGCAGTGCG 
GTGAAGCTTG AAAAGGTGGA GCTAAGTTCT GTAAGAACTG CATCAACAAT GGTTGCGGCT 
CCGCCTACAG CAGTTACGCC TGCTCCTGCC ACAACTGTTG CCCCGGCTCC AACCTCTACA 
CCTGCTCCAG CTGCTGCCCC AACTCCTGCT CCTGCTCCTG CCCCTGCACT CTTCCCTCCA 
ACAAAACTGG TGATCAAGCA AGGTGGAGGA GGAGCTTCAG TCTCCTGGTC CACCAGCTCA 
ACCCCTACGC CTGCTCCGGT GGTGCGACCG CCTGCTGAGC CTGTCGCACC AAGTGCCTCC 
TTCAGCCGCA CTCCGTCCTC CCGCCCTGCT GACGACGACG ATGACGTTGC ACTTCCCCAA 
CGGACCAGCA AACCACCCAT CAAGACCTAC GAGGCACGTC GGCGGATAGG TTTGAAGCTG 
AAGATCAAGC AAGAGGCGGG GTTAAGTAAG GTGGTTCACA ACACTGCATT AGATCCTGTC 
CATTCGCAAT CACAACTCAC ACCACAGACG CCCACCCCCG AGCAGCCGCA GAAAGTAAAG 
CCACACGTAA CTCTTCCCTC CACCGTCATT AGAACTCAAC CCACAACCTC TCAATCCACC 
TCCTCCTCCA CTGTCACCAC AGTAACCACA CACACAGCCT CCGCCTCCAG TAGCGCAACT 
TCCTCGAGCG GGGCATCGTC TTCATTCTCC ACCTGGTCTT CATCGTCACC TTCTACGTCG 
ACCGCGCAGA TGAACGGCAC CCTGGAGCAC CATGAGGTAG GTGGGGTCAA ACGTAACCCA 
GCGTCCACAG CAACGCCACC ACTAACAACG TGCAGGCTCC CGCTCCGAAA GACATACCGT 
GAGAACATCA GTCCCCGTCA CAGGCCTGGA GTACCAGGAG GTGGTGGAGA TACACTACCC 
ATTCTGCCTG CAGGACCTCC TGTTACCTCT TCGCCCCAAC CGCAAAGCTC GTCCCCTCAA 
CCCGAACGAA CTGTGATAGC CAGTGTGAAA CTGGAGCGGC AAGGTGGACA CGGGCGATCT 
CACCCCCATG CGGAGTCACA AAGCCTGGCA GCCGTTGAAG ACGTCCTTTA TCGGGGAATA 
AAAAACGCGT ACCAACATCA TCGGGAGTTT GATAAAGACG ATGAGGATGA TATGGAGGAA 
GGTGGTGGTG GTGGTGGTGC TTTGGGGCGG TTAAAGGGCA TAGGAAGCAA ACACCGGGAA 
GGGGGACGGG GCGCCTTCAG GATGGATCAG CATGCCCCAG GGCCGCCCTC TCCCGGAGAG 
TCTTCCTGCA CAAGAGACTC GTCACTTCCT GCCAAACGCT GCAAGTCGGA CTCTCCGGAC 
ATGGACAATG CAAGCTTCTC CAGCGGCAGC CCGCCGCCTG ACGACTCGCT AAATGAGCAC 
CTGCAGTGTG CCATAGACAG TATACTAAAC CTCCAACAGG GCCCTCCTGG ACACGGAGGC 
TCAGGGAGAG GGGATTTAGG GAGGGTTCAT GGGGCAAACC TGCCTAACCA GCACCAAACC 
CATCCCTCCT ACAGACAGTC CATGCCTCCC CTCTCAACAC AGCCCTCGTC CGCCCCCATG 
TCCCAGCATT CACAGGTAGG AGGGCGAGGG CAGAATGGAA ATCTGGTTTC ACAGACGCAC 
AGTAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159310.1
CDS	951..6419
Translation	MDDEDGRCLLDVICDPQALNDFLHGSETQLDTDDLLDGSGDPSSSFFSSTGGHVPEVPAQVQLSTSEPGLPRVSVDLDFLEDDDILGGSPGGDNGSNGVGTNHEPCDILQQSLAEANITEQSLQEADAELDLSSFGLTGLTQVVQPLPDAGLSGVGIGGATQIFPNQGTPTAPSNPTPDMLGSVLAHPGLQLQQQVMNKAISVQPFVQQVGLGNVTLQPISSLQALPNGSQSGPLGIGQIQVVGQPTVMTINPSGQQILTKTMGGYQLHQPGPEAASAGTQAGLGSSVLSSGGGLVIQGGKATLGSPALNGPAVCLGSTNTNNSATTMATAGGIVGFSNASLGAGIGSQTQPQGQIMQNVIIQRTPTPIQPKPPQGGTIQPKIFKQQQQQLPAPHALPNDANKALGVQQIPVSAGQNVTFLTGKPGSNVVLSTQAASQGTQFSQALFKQQGPQTSGKPLSVHLLNQQGSIVIPSQTVLQGQNHQFLLPGLQAGGQILAQHPGGHIITSQGPGGQIIANQILTANQNINLGQVLASQGHPGAAHILSGHIQLQPGQMGQPALFQMPVSLAHTQTQSHPVTGHVQTVIQGMPIQNSLSVESLSPAVSLQTLQQSGGVPNNGSSGSTAMAPCQPGEGITVLGGSSDSAAQPAQTQPQPSILTVQTTPPVSVAASVPSSSSPSPTMATSASTMVGLCSQAQHSPGKVLFTSPGSGMILSQEPLQMFLHQEQQHHAGKDPPAAVGVPASVIVSGSSSGPVPSGHDNLLAETRQRQSPSPSLGPAHMATVVNKVPSSAHSQSIKIQNASQSQPVVTPSPAPTLSDSPQPAQVSPLTLGQQIQSPHQQSRPPSQPQPPSQAQTPSRSCTPSSLPPLFIIHNQMGGSPQPAPPPQQQQPQQLQVQLQPQVLPQPAALQPDVPPSSCSPKPPQPLPTQFQFQPPVSSSSPAAAVKQQVTVVPGLTAEQQHHLQLVSAQLQTMSSITQPSPQQKQLLEKLHQVQQNILLQAKQQAQAQAQASQQQATNQFNKMPDQPSVQAATSSTATGAIQAPVQSLLPQKSVLVKPSTTGANDTQVFSAVSAGATVNTGITTPNLAQAVQAKPGVISSVSGLTLGKAGLQIQVLGAGLSQMPAPPLPAPQTQTSTLKRPFSMEPSKEARMLEQLRKQQGSVLHPDYSSPFRSFEDTLHRLLPYHLYQGTASSPEDCRKVDDEFENVSSQLLKRTQAMLDKYRHLLFEESKRLGPSAEMVMIDRMFIQEEKVALSQDRVLAKEKPEEFVANSCLVDSSAVKLEKVELSSVRTASTMVAAPPTAVTPAPATTVAPAPTSTPAPAAAPTPAPAPAPALFPPTKLVIKQGGGGASVSWSTSSTPTPAPVVRPPAEPVAPSASFSRTPSSRPADDDDDVALPQRTSKPPIKTYEARRRIGLKLKIKQEAGLSKVVHNTALDPVHSQSQLTPQTPTPEQPQKVKPHVTLPSTVIRTQPTTSQSTSSSTVTTVTTHTASASSSATSSSGASSSFSTWSSSSPSTSTAQMNGTLEHHEVGGVKRNPASTATPPLTTCRLPLRKTYRENISPRHRPGVPGGGGDTLPILPAGPPVTSSPQPQSSSPQPERTVIASVKLERQGGHGRSHPHAESQSLAAVEDVLYRGIKNAYQHHREFDKDDEDDMEEGGGGGGALGRLKGIGSKHREGGRGAFRMDQHAPGPPSPGESSCTRDSSLPAKRCKSDSPDMDNASFSSGSPPPDDSLNEHLQCAIDSILNLQQGPPGHGGSGRGDLGRVHGANLPNQHQTHPSYRQSMPPLSTQPSSAPMSQHSQVGGRGQNGNLVSQTHSR

Target ORF information:

RefSeq Version	XM_005159253.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio BRD4 interacting chromatin remodeling complex associated protein (bicra), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159253.4

ORF Insert Sequence:

ATGGATGATG AAGATGGCAG GTGCTTGCTA GATGTAATTT GTGACCCACA AGCACTCAAC 
GATTTTCTTC ATGGATCAGA AACACAACTG GACACTGATG ACCTATTGGA TGGCTCAGGT 
GACCCGTCCA GCTCGTTCTT CTCCAGCACT GGGGGCCATG TTCCTGAGGT TCCGGCACAG 
GTTCAGCTGT CGACAAGTGA GCCGGGCCTT CCAAGAGTCA GCGTTGATTT GGACTTCTTG 
GAGGACGATG ACATATTGGG GGGATCGCCA GGAGGAGACA ATGGAAGTAA TGGTGTTGGG 
ACAAATCATG AGCCCTGTGA CATTTTGCAG CAGAGCTTGG CGGAGGCTAA CATCACAGAG 
CAGAGCCTTC AAGAGGCAGA TGCTGAGCTT GACCTCAGTT CCTTTGGGCT AACTGGCCTT 
ACGCAGGTGG TTCAACCCCT GCCTGATGCC GGCCTGTCTG GGGTGGGCAT TGGAGGTGCA 
ACTCAGATTT TTCCAAACCA AGGCACCCCT ACCGCACCCT CAAATCCCAC TCCAGACATG 
CTTGGTTCTG TCCTGGCACA TCCAGGTCTG CAGCTTCAAC AACAGGTCAT GAACAAGGCT 
ATCAGCGTCC AGCCTTTTGT ACAACAAGTC GGACTTGGAA ATGTAACACT TCAGCCAATT 
TCGAGCCTTC AGGCCCTACC GAACGGCAGC CAGTCTGGAC CGTTGGGCAT TGGACAAATT 
CAAGTGGTGG GTCAGCCCAC TGTAATGACT ATAAATCCAT CTGGGCAGCA AATCTTGACG 
AAAACCATGG GTGGCTACCA ACTGCATCAA CCTGGGCCGG AGGCAGCAAG CGCTGGAACG 
CAGGCTGGGC TTGGAAGCTC TGTTTTGAGT TCAGGAGGTG GACTTGTAAT TCAAGGGGGC 
AAGGCCACTC TCGGGTCCCC TGCCTTAAAT GGGCCAGCTG TCTGCTTAGG CAGCACAAAT 
ACCAACAACA GTGCAACTAC AATGGCCACT GCTGGTGGTA TAGTGGGTTT TAGTAATGCC 
TCTTTGGGTG CAGGAATTGG ATCTCAGACC CAGCCTCAAG GCCAAATCAT GCAGAATGTC 
ATCATCCAGC GAACGCCAAC GCCAATACAG CCCAAACCTC CTCAAGGGGG TACAATCCAA 
CCCAAAATCT TCAAGCAACA ACAACAACAG CTTCCTGCCC CACATGCCTT GCCAAATGAT 
GCTAATAAAG CTCTGGGGGT ACAGCAAATC CCAGTGTCAG CTGGTCAGAA TGTAACGTTC 
CTCACCGGGA AGCCTGGCTC TAACGTAGTT TTGAGCACAC AGGCAGCGTC GCAGGGAACA 
CAGTTTTCTC AAGCGCTCTT TAAACAGCAG GGCCCTCAAA CTTCAGGCAA ACCCCTCAGT 
GTTCACTTGT TAAACCAACA GGGCAGCATT GTCATTCCCT CGCAAACTGT CCTGCAGGGC 
CAAAACCACC AGTTCCTCTT ACCAGGGCTT CAAGCAGGAG GCCAGATCCT GGCTCAGCAT 
CCTGGTGGAC ACATCATAAC CAGTCAGGGG CCTGGAGGGC AGATAATCGC TAATCAGATC 
TTGACAGCCA ATCAGAACAT CAACTTGGGA CAGGTGTTGG CTTCACAGGG CCACCCTGGT 
GCAGCCCACA TTCTCTCTGG ACACATCCAG CTCCAGCCTG GTCAGATGGG TCAGCCTGCA 
CTGTTCCAAA TGCCGGTGTC TTTGGCGCAT ACTCAGACAC AATCTCATCC GGTAACCGGG 
CATGTACAAA CGGTCATTCA GGGCATGCCT ATACAGAATT CCCTGTCTGT GGAGAGCCTT 
AGCCCAGCAG TCAGTTTACA GACGTTGCAG CAGTCTGGCG GCGTCCCTAA TAATGGTAGC 
AGTGGATCGA CGGCAATGGC GCCATGCCAG CCAGGAGAGG GTATCACTGT ATTGGGCGGC 
TCTTCTGACT CTGCTGCTCA ACCAGCACAG ACGCAACCTC AGCCCTCAAT TCTCACGGTT 
CAGACGACGC CTCCAGTTTC AGTAGCAGCT TCGGTTCCTT CCTCTTCATC TCCATCCCCA 
ACGATGGCCA CGTCTGCATC CACAATGGTG GGTCTGTGTT CACAGGCCCA GCACAGCCCT 
GGAAAAGTGT TGTTCACGTC ACCTGGTTCC GGCATGATCC TGAGCCAGGA GCCTCTTCAA 
ATGTTCCTGC ACCAGGAGCA ACAGCATCAC GCAGGAAAAG ACCCACCTGC CGCTGTGGGT 
GTACCTGCAT CTGTTATCGT CAGCGGCAGC AGTTCTGGTC CCGTCCCTTC AGGCCATGAC 
AACTTGTTAG CTGAGACTCG GCAGAGGCAG AGTCCCAGTC CCTCCCTTGG TCCTGCCCAC 
ATGGCAACAG TGGTTAACAA GGTTCCATCG TCAGCACATT CACAGTCTAT AAAGATCCAA 
AACGCCTCCC AATCTCAGCC TGTGGTCACC CCTTCGCCAG CACCCACACT GTCCGACAGC 
CCTCAACCTG CTCAAGTTTC CCCTCTCACT CTCGGGCAGC AGATCCAGTC TCCCCATCAA 
CAGTCTCGGC CTCCCTCGCA GCCCCAGCCT CCTTCACAGG CACAGACTCC TTCACGCTCA 
TGCACCCCTT CCTCTCTACC GCCTCTCTTC ATCATTCACA ATCAGATGGG AGGTTCTCCA 
CAGCCGGCTC CACCACCTCA GCAACAGCAG CCTCAGCAGT TACAAGTGCA GCTCCAGCCT 
CAAGTTCTTC CTCAGCCTGC TGCTCTGCAG CCAGACGTAC CTCCTTCCTC GTGCTCCCCA 
AAGCCTCCGC AGCCACTTCC CACACAGTTC CAGTTCCAGC CCCCAGTCTC CAGCTCGTCT 
CCAGCTGCAG CAGTGAAACA GCAGGTGACG GTGGTGCCTG GGCTGACCGC AGAACAGCAG 
CATCACCTAC AGCTGGTCAG TGCGCAGCTG CAGACCATGT CATCAATCAC ACAGCCCTCC 
CCTCAACAAA AGCAGCTCTT GGAAAAACTT CACCAGGTCC AGCAAAACAT ACTTTTGCAG 
GCTAAGCAGC AAGCTCAGGC ACAAGCCCAG GCCTCTCAGC AACAGGCTAC AAACCAGTTC 
AACAAAATGC CAGATCAGCC TTCGGTCCAA GCTGCGACTT CTTCAACGGC CACTGGAGCC 
ATCCAAGCAC CAGTCCAGTC TCTTCTGCCG CAGAAGTCTG TGCTTGTCAA GCCCTCTACT 
ACAGGTGCAA ATGACACACA AGTGTTTTCT GCGGTGTCTG CTGGGGCTAC GGTGAACACG 
GGAATCACAA CACCAAACCT TGCACAGGCT GTCCAGGCAA AGCCAGGCGT CATAAGTTCG 
GTTAGTGGAC TAACTCTGGG CAAAGCAGGT TTGCAGATTC AGGTGTTAGG TGCTGGACTC 
TCTCAAATGC CTGCACCTCC ACTACCTGCT CCCCAAACAC AGACATCAAC ATTAAAAAGG 
CCCTTTAGTA TGGAACCAAG CAAAGAAGCT AGGATGCTGG AACAGCTGCG AAAACAGCAA 
GGCTCAGTTC TCCATCCTGA CTACAGCTCT CCTTTCCGCT CTTTTGAGGA CACACTTCAT 
CGGCTGCTGC CATACCATCT GTACCAAGGC ACGGCTTCAT CTCCGGAAGA CTGTCGTAAA 
GTGGATGATG AATTTGAGAA CGTCTCAAGC CAGCTCTTGA AACGCACACA AGCAATGCTG 
GATAAATATC GTCACTTGCT TTTCGAGGAA TCAAAGAGGC TGGGTCCCTC AGCAGAGATG 
GTTATGATTG ACCGGATGTT CATTCAAGAG GAAAAGGTTG CTCTGAGTCA GGACAGGGTC 
CTAGCCAAGG AGAAACCAGA GGAATTTGTG GCCAATTCGT GTTTAGTGGA CAGCAGTGCG 
GTGAAGCTTG AAAAGGTGGA GCTAAGTTCT GTAAGAACTG CATCAACAAT GGTTGCGGCT 
CCGCCTACAG CAGTTACGCC TGCTCCTGCC ACAACTGTTG CCCCGGCTCC AACCTCTACA 
CCTGCTCCAG CTGCTGCCCC AACTCCTGCT CCTGCTCCTG CCCCTGCACT CTTCCCTCCA 
ACAAAACTGG TGATCAAGCA AGGTGGAGGA GGAGCTTCAG TCTCCTGGTC CACCAGCTCA 
ACCCCTACGC CTGCTCCGGT GGTGCGACCG CCTGCTGAGC CTGTCGCACC AAGTGCCTCC 
TTCAGCCGCA CTCCGTCCTC CCGCCCTGCT GACGACGACG ATGACGTTGC ACTTCCCCAA 
CGGACCAGCA AACCACCCAT CAAGACCTAC GAGGCACGTC GGCGGATAGG TTTGAAGCTG 
AAGATCAAGC AAGAGGCGGG GTTAAGTAAG GTGGTTCACA ACACTGCATT AGATCCTGTC 
CATTCGCAAT CACAACTCAC ACCACAGACG CCCACCCCCG AGCAGCCGCA GAAAGTAAAG 
CCACACGTAA CTCTTCCCTC CACCGTCATT AGAACTCAAC CCACAACCTC TCAATCCACC 
TCCTCCTCCA CTGTCACCAC AGTAACCACA CACACAGCCT CCGCCTCCAG TAGCGCAACT 
TCCTCGAGCG GGGCATCGTC TTCATTCTCC ACCTGGTCTT CATCGTCACC TTCTACGTCG 
ACCGCGCAGA TGAACGGCAC CCTGGAGCAC CATGAGGTAG GTGGGGTCAA ACGTAACCCA 
GCGTCCACAG CAACGCCACC ACTAACAACG TGCAGGCTCC CGCTCCGAAA GACATACCGT 
GAGAACATCA GTCCCCGTCA CAGGCCTGGA GTACCAGGAG GTGGTGGAGA TACACTACCC 
ATTCTGCCTG CAGGACCTCC TGTTACCTCT TCGCCCCAAC CGCAAAGCTC GTCCCCTCAA 
CCCGAACGAA CTGTGATAGC CAGTGTGAAA CTGGAGCGGC AAGGTGGACA CGGGCGATCT 
CACCCCCATG CGGAGTCACA AAGCCTGGCA GCCGTTGAAG ACGTCCTTTA TCGGGGAATA 
AAAAACGCGT ACCAACATCA TCGGGAGTTT GATAAAGACG ATGAGGATGA TATGGAGGAA 
GGTGGTGGTG GTGGTGGTGC TTTGGGGCGG TTAAAGGGCA TAGGAAGCAA ACACCGGGAA 
GGGGGACGGG GCGCCTTCAG GATGGATCAG CATGCCCCAG GGCCGCCCTC TCCCGGAGAG 
TCTTCCTGCA CAAGAGACTC GTCACTTCCT GCCAAACGCT GCAAGTCGGA CTCTCCGGAC 
ATGGACAATG CAAGCTTCTC CAGCGGCAGC CCGCCGCCTG ACGACTCGCT AAATGAGCAC 
CTGCAGTGTG CCATAGACAG TATACTAAAC CTCCAACAGG GCCCTCCTGG ACACGGAGGC 
TCAGGGAGAG GGGATTTAGG GAGGGTTCAT GGGGCAAACC TGCCTAACCA GCACCAAACC 
CATCCCTCCT ACAGACAGTC CATGCCTCCC CTCTCAACAC AGCCCTCGTC CGCCCCCATG 
TCCCAGCATT CACAGGTAGG AGGGCGAGGG CAGAATGGAA ATCTGGTTTC ACAGACGCAC 
AGTAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bicra ( XM_001922789.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001922824.3 Danio rerio glioma tumor suppressor candidate region gene 1 (gltscr1), transcript variant X3, mRNA.

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CloneID	ODa23560
Clone ID Related Accession (Same CDS sequence)	XM_001922789.6 , XM_001922789.7
Accession Version	XM_001922789.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	glioma tumor suppressor candidate region gene 1 protein isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001922789.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATG AAGATGGCAG GTGCTTGCTA GATGTAATTT GTGACCCACA AGCACTCAAC 
GATTTTCTTC ATGGATCAGA AACACAAGGC CATGTTCCTG AGGTTCCGGC ACAGGTTCAG 
CTGTCGACAA GTGAGCCGGG CCTTCCAAGA GTCAGCGTTG ATTTGGACTT CTTGGAGGAC 
GATGACATAT TGGGGGGATC GCCAGGAGGA GACAATGGAA GTAATGGTGT TGGGACAAAT 
CATGAGCCCT GTGACATTTT GCAGCAGAGC TTGGCGGAGG CTAACATCAC AGAGCAGAGC 
CTTCAAGAGG CAGATGCTGA GCTTGACCTC AGTTCCTTTG GGCTAACTGG CCTTACGCAG 
GTGGTTCAAC CCCTGCCTGA TGCCGGCCTG TCTGGGGTGG GCATTGGAGG TGCAACTCAG 
ATTTTTCCAA ACCAAGGCAC CCCTACCGCA CCCTCAAATC CCACTCCAGA CATGCTTGGT 
TCTGTCCTGG CACATCCAGG TCTGCAGCTT CAACAACAGG TCATGAACAA GGCTATCAGC 
GTCCAGCCTT TTGTACAACA AGTCGGACTT GGAAATGTAA CACTTCAGCC AATTTCGAGC 
CTTCAGGCCC TACCGAACGG CAGCCAGTCT GGACCGTTGG GCATTGGACA AATTCAAGTG 
GTGGGTCAGC CCACTGTAAT GACTATAAAT CCATCTGGGC AGCAAATCTT GACGAAAACC 
ATGGGTGGCT ACCAACTGCA TCAACCTGGG CCGGAGGCAG CAAGCGCTGG AACGCAGGCT 
GGGCTTGGAA GCTCTGTTTT GAGTTCAGGA GGTGGACTTG TAATTCAAGG GGGCAAGGCC 
ACTCTCGGGT CCCCTGCCTT AAATGGGCCA GCTGTCTGCT TAGGCAGCAC AAATACCAAC 
AACAGTGCAA CTACAATGGC CACTGCTGGT GGTATAGTGG GTTTTAGTAA TGCCTCTTTG 
GGTGCAGGAA TTGGATCTCA GACCCAGCCT CAAGGCCAAA TCATGCAGAA TGTCATCATC 
CAGCGAACGC CAACGCCAAT ACAGCCCAAA CCTCCTCAAG GGGGTACAAT CCAACCCAAA 
ATCTTCAAGC AACAACAACA ACAGCTTCCT GCCCCACATG CCTTGCCAAA TGATGCTAAT 
AAAGCTCTGG GGGTACAGCA AATCCCAGTG TCAGCTGGTC AGAATGTAAC GTTCCTCACC 
GGGAAGCCTG GCTCTAACGT AGTTTTGAGC ACACAGGCAG CGTCGCAGGG AACACAGTTT 
TCTCAAGCGC TCTTTAAACA GCAGGGCCCT CAAACTTCAG GCAAACCCCT CAGTGTTCAC 
TTGTTAAACC AACAGGGCAG CATTGTCATT CCCTCGCAAA CTGTCCTGCA GGGCCAAAAC 
CACCAGTTCC TCTTACCAGG GCTTCAAGCA GGAGGCCAGA TCCTGGCTCA GCATCCTGGT 
GGACACATCA TAACCAGTCA GGGGCCTGGA GGGCAGATAA TCGCTAATCA GATCTTGACA 
GCCAATCAGA ACATCAACTT GGGACAGGTG TTGGCTTCAC AGGGCCACCC TGGTGCAGCC 
CACATTCTCT CTGGACACAT CCAGCTCCAG CCTGGTCAGA TGGGTCAGCC TGCACTGTTC 
CAAATGCCGG TGTCTTTGGC GCATACTCAG ACACAATCTC ATCCGGTAAC CGGGCATGTA 
CAAACGGTCA TTCAGGGCAT GCCTATACAG AATTCCCTGT CTGTGGAGAG CCTTAGCCCA 
GCAGTCAGTT TACAGACGTT GCAGCAGTCT GGCGGCGTCC CTAATAATGG TAGCAGTGGA 
TCGACGGCAA TGGCGCCATG CCAGCCAGGA GAGGGTATCA CTGTATTGGG CGGCTCTTCT 
GACTCTGCTG CTCAACCAGC ACAGACGCAA CCTCAGCCCT CAATTCTCAC GGTTCAGACG 
ACGCCTCCAG TTTCAGTAGC AGCTTCGGTT CCTTCCTCTT CATCTCCATC CCCAACGATG 
GCCACGTCTG CATCCACAAT GGTGGGTCTG TGTTCACAGG CCCAGCACAG CCCTGGAAAA 
GTGTTGTTCA CGTCACCTGG TTCCGGCATG ATCCTGAGCC AGGAGCCTCT TCAAATGTTC 
CTGCACCAGG AGCAACAGCA TCACGCAGGA AAAGACCCAC CTGCCGCTGT GGGTGTACCT 
GCATCTGTTA TCGTCAGCGG CAGCAGTTCT GGTCCCGTCC CTTCAGGCCA TGACAACTTG 
TTAGCTGAGA CTCGGCAGAG GCAGAGTCCC AGTCCCTCCC TTGGTCCTGC CCACATGGCA 
ACAGTGGTTA ACAAGGTTCC ATCGTCAGCA CATTCACAGT CTATAAAGAT CCAAAACGCC 
TCCCAATCTC AGCCTGTGGT CACCCCTTCG CCAGCACCCA CACTGTCCGA CAGCCCTCAA 
CCTGCTCAAG TTTCCCCTCT CACTCTCGGG CAGCAGATCC AGTCTCCCCA TCAACAGTCT 
CGGCCTCCCT CGCAGCCCCA GCCTCCTTCA CAGGCACAGA CTCCTTCACG CTCATGCACC 
CCTTCCTCTC TACCGCCTCT CTTCATCATT CACAATCAGA TGGGAGGTTC TCCACAGCCG 
GCTCCACCAC CTCAGCAACA GCAGCCTCAG CAGTTACAAG TGCAGCTCCA GCCTCAAGTT 
CTTCCTCAGC CTGCTGCTCT GCAGCCAGAC GTACCTCCTT CCTCGTGCTC CCCAAAGCCT 
CCGCAGCCAC TTCCCACACA GTTCCAGTTC CAGCCCCCAG TCTCCAGCTC GTCTCCAGCT 
GCAGCAGTGA AACAGCAGGT GACGGTGGTG CCTGGGCTGA CCGCAGAACA GCAGCATCAC 
CTACAGCTGG TCAGTGCGCA GCTGCAGACC ATGTCATCAA TCACACAGCC CTCCCCTCAA 
CAAAAGCAGC TCTTGGAAAA ACTTCACCAG GTCCAGCAAA ACATACTTTT GCAGGCTAAG 
CAGCAAGCTC AGGCACAAGC CCAGGCCTCT CAGCAACAGG CTACAAACCA GTTCAACAAA 
ATGCCAGATC AGCCTTCGGT CCAAGCTGCG ACTTCTTCAA CGGCCACTGG AGCCATCCAA 
GCACCAGTCC AGTCTCTTCT GCCGCAGAAG TCTGTGCTTG TCAAGCCCTC TACTACAGGT 
GCAAATGACA CACAAGTGTT TTCTGCGGTG TCTGCTGGGG CTACGGTGAA CACGGGAATC 
ACAACACCAA ACCTTGCACA GGCTGTCCAG GCAAAGCCAG GCGTCATAAG TTCGGTTAGT 
GGACTAACTC TGGGCAAAGC AGGTTTGCAG ATTCAGGTGT TAGGTGCTGG ACTCTCTCAA 
ATGCCTGCAC CTCCACTACC TGCTCCCCAA ACACAGACAT CAACATTAAA AAGGCCCTTT 
AGTATGGAAC CAAGCAAAGA AGCTAGGATG CTGGAACAGC TGCGAAAACA GCAAGGCTCA 
GTTCTCCATC CTGACTACAG CTCTCCTTTC CGCTCTTTTG AGGACACACT TCATCGGCTG 
CTGCCATACC ATCTGTACCA AGGCACGGCT TCATCTCCGG AAGACTGTCG TAAAGTGGAT 
GATGAATTTG AGAACGTCTC AAGCCAGCTC TTGAAACGCA CACAAGCAAT GCTGGATAAA 
TATCGTCACT TGCTTTTCGA GGAATCAAAG CAGAGGCTGG GTCCCTCAGC AGAGATGGTT 
ATGATTGACC GGATGTTCAT TCAAGAGGAA AAGGTTGCTC TGAGTCAGGA CAGGGTCCTA 
GCCAAGGAGA AACCAGAGGA ATTTGTGGCC AATTCGTGTT TAGTGGACAG CAGTGCGGTG 
AAGCTTGAAA AGGTGGAGCT AAGTTCTGTA AGAACTGCAT CAACAATGGT TGCGGCTCCG 
CCTACAGCAG TTACGCCTGC TCCTGCCACA ACTGTTGCCC CGGCTCCAAC CTCTACACCT 
GCTCCAGCTG CTGCCCCAAC TCCTGCTCCT GCTCCTGCCC CTGCACTCTT CCCTCCAACA 
AAACTGGTGA TCAAGCAAGG TGGAGGAGGA GCTTCAGTCT CCTGGTCCAC CAGCTCAACC 
CCTACGCCTG CTCCGGTGGT GCGACCGCCT GCTGAGCCTG TCGCACCAAG TGCCTCCTTC 
AGCCGCACTC CGTCCTCCCG CCCTGCTGAC GACGACGATG ACGTTGCACT TCCCCAACGG 
ACCAGCAAAC CACCCATCAA GACCTACGAG GCACGTCGGC GGATAGGTTT GAAGCTGAAG 
ATCAAGCAAG AGGCGGGGTT AAGTAAGGTG GTTCACAACA CTGCATTAGA TCCTGTCCAT 
TCGCAATCAC AACTCACACC ACAGACGCCC ACCCCCGAGC AGCCGCAGAA AGTAAAGCCA 
CACGTAACTC TTCCCTCCAC CGTCATTAGA ACTCAACCCA CAACCTCTCA ATCCACCTCC 
TCCTCCACTG TCACCACAGT AACCACACAC ACAGCCTCCG CCTCCAGTAG CGCAACTTCC 
TCGAGCGGGG CATCGTCTTC ATTCTCCACC TGGTCTTCAT CGTCACCTTC TACGTCGACC 
GCGCAGATGA ACGGCACCCT GGAGCACCAT GAGGTAGGTG GGGTCAAACG TAACCCAGCG 
TCCACAGCAA CGCCACCACT AACAACGTGC AGGCTCCCGC TCCGAAAGAC ATACCGTGAG 
AACATCAGTC CCCGTCACAG GCCTGGAGTA CCAGGAGGTG GTGGAGATAC ACTACCCATT 
CTGCCTGCAG GACCTCCTGT TACCTCTTCG CCCCAACCGC AAAGCTCGTC CCCTCAACCC 
GAACGAACTG TGATAGCCAG TGTGAAACTG GAGCGGCAAG GTGGACACGG GCGATCTCAC 
CCCCATGCGG AGTCACAAAG CCTGGCAGCC GTTGAAGACG TCCTTTATCG GGGAATAAAA 
AACGCGTACC AACATCATCG GGAGTTTGAT AAAGACGATG AGGATGATAT GGAGGAAGGT 
GGTGGTGGTG GTGGTGCTTT GGGGCGGTTA AAGGGCATAG GAAGCAAACA CCGGGAAGGG 
GGACGGGGCG CCTTCAGGAT GGATCAGCAT GCCCCAGGGC CGCCCTCTCC CGGAGAGTCT 
TCCTGCACAA GAGACTCGTC ACTTCCTGCC AAACGCTGCA AGTCGGACTC TCCGGACATG 
GACAATGCAA GCTTCTCCAG CGGCAGCCCG CCGCCTGACG ACTCGCTAAA TGAGCACCTG 
CAGTGTGCCA TAGACAGTAT ACTAAACCTC CAACAGGGCC CTCCTGGACA CGGAGGCTCA 
GGGAGAGGGG ATTTAGGGAG GGTTCATGGG GCAAACCTGC CTAACCAGCA CCAAACCCAT 
CCCTCCTACA GACAGTCCAT GCCTCCCCTC TCAACACAGC CCTCGTCCGC CCCCATGTCC 
CAGCATTCAC AGGTAGGAGG GCGAGGGCAG AATGGAAATC TGGTTTCACA GACGCACAGT 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001922824.3
CDS	1148..6553
Translation	MDDEDGRCLLDVICDPQALNDFLHGSETQGHVPEVPAQVQLSTSEPGLPRVSVDLDFLEDDDILGGSPGGDNGSNGVGTNHEPCDILQQSLAEANITEQSLQEADAELDLSSFGLTGLTQVVQPLPDAGLSGVGIGGATQIFPNQGTPTAPSNPTPDMLGSVLAHPGLQLQQQVMNKAISVQPFVQQVGLGNVTLQPISSLQALPNGSQSGPLGIGQIQVVGQPTVMTINPSGQQILTKTMGGYQLHQPGPEAASAGTQAGLGSSVLSSGGGLVIQGGKATLGSPALNGPAVCLGSTNTNNSATTMATAGGIVGFSNASLGAGIGSQTQPQGQIMQNVIIQRTPTPIQPKPPQGGTIQPKIFKQQQQQLPAPHALPNDANKALGVQQIPVSAGQNVTFLTGKPGSNVVLSTQAASQGTQFSQALFKQQGPQTSGKPLSVHLLNQQGSIVIPSQTVLQGQNHQFLLPGLQAGGQILAQHPGGHIITSQGPGGQIIANQILTANQNINLGQVLASQGHPGAAHILSGHIQLQPGQMGQPALFQMPVSLAHTQTQSHPVTGHVQTVIQGMPIQNSLSVESLSPAVSLQTLQQSGGVPNNGSSGSTAMAPCQPGEGITVLGGSSDSAAQPAQTQPQPSILTVQTTPPVSVAASVPSSSSPSPTMATSASTMVGLCSQAQHSPGKVLFTSPGSGMILSQEPLQMFLHQEQQHHAGKDPPAAVGVPASVIVSGSSSGPVPSGHDNLLAETRQRQSPSPSLGPAHMATVVNKVPSSAHSQSIKIQNASQSQPVVTPSPAPTLSDSPQPAQVSPLTLGQQIQSPHQQSRPPSQPQPPSQAQTPSRSCTPSSLPPLFIIHNQMGGSPQPAPPPQQQQPQQLQVQLQPQVLPQPAALQPDVPPSSCSPKPPQPLPTQFQFQPPVSSSSPAAAVKQQVTVVPGLTAEQQHHLQLVSAQLQTMSSITQPSPQQKQLLEKLHQVQQNILLQAKQQAQAQAQASQQQATNQFNKMPDQPSVQAATSSTATGAIQAPVQSLLPQKSVLVKPSTTGANDTQVFSAVSAGATVNTGITTPNLAQAVQAKPGVISSVSGLTLGKAGLQIQVLGAGLSQMPAPPLPAPQTQTSTLKRPFSMEPSKEARMLEQLRKQQGSVLHPDYSSPFRSFEDTLHRLLPYHLYQGTASSPEDCRKVDDEFENVSSQLLKRTQAMLDKYRHLLFEESKQRLGPSAEMVMIDRMFIQEEKVALSQDRVLAKEKPEEFVANSCLVDSSAVKLEKVELSSVRTASTMVAAPPTAVTPAPATTVAPAPTSTPAPAAAPTPAPAPAPALFPPTKLVIKQGGGGASVSWSTSSTPTPAPVVRPPAEPVAPSASFSRTPSSRPADDDDDVALPQRTSKPPIKTYEARRRIGLKLKIKQEAGLSKVVHNTALDPVHSQSQLTPQTPTPEQPQKVKPHVTLPSTVIRTQPTTSQSTSSSTVTTVTTHTASASSSATSSSGASSSFSTWSSSSPSTSTAQMNGTLEHHEVGGVKRNPASTATPPLTTCRLPLRKTYRENISPRHRPGVPGGGGDTLPILPAGPPVTSSPQPQSSSPQPERTVIASVKLERQGGHGRSHPHAESQSLAAVEDVLYRGIKNAYQHHREFDKDDEDDMEEGGGGGGALGRLKGIGSKHREGGRGAFRMDQHAPGPPSPGESSCTRDSSLPAKRCKSDSPDMDNASFSSGSPPPDDSLNEHLQCAIDSILNLQQGPPGHGGSGRGDLGRVHGANLPNQHQTHPSYRQSMPPLSTQPSSAPMSQHSQVGGRGQNGNLVSQTHSR

Target ORF information:

RefSeq Version	XM_001922789.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio glioma tumor suppressor candidate region gene 1 (gltscr1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001922789.6

ORF Insert Sequence:

ATGGATGATG AAGATGGCAG GTGCTTGCTA GATGTAATTT GTGACCCACA AGCACTCAAC 
GATTTTCTTC ATGGATCAGA AACACAAGGC CATGTTCCTG AGGTTCCGGC ACAGGTTCAG 
CTGTCGACAA GTGAGCCGGG CCTTCCAAGA GTCAGCGTTG ATTTGGACTT CTTGGAGGAC 
GATGACATAT TGGGGGGATC GCCAGGAGGA GACAATGGAA GTAATGGTGT TGGGACAAAT 
CATGAGCCCT GTGACATTTT GCAGCAGAGC TTGGCGGAGG CTAACATCAC AGAGCAGAGC 
CTTCAAGAGG CAGATGCTGA GCTTGACCTC AGTTCCTTTG GGCTAACTGG CCTTACGCAG 
GTGGTTCAAC CCCTGCCTGA TGCCGGCCTG TCTGGGGTGG GCATTGGAGG TGCAACTCAG 
ATTTTTCCAA ACCAAGGCAC CCCTACCGCA CCCTCAAATC CCACTCCAGA CATGCTTGGT 
TCTGTCCTGG CACATCCAGG TCTGCAGCTT CAACAACAGG TCATGAACAA GGCTATCAGC 
GTCCAGCCTT TTGTACAACA AGTCGGACTT GGAAATGTAA CACTTCAGCC AATTTCGAGC 
CTTCAGGCCC TACCGAACGG CAGCCAGTCT GGACCGTTGG GCATTGGACA AATTCAAGTG 
GTGGGTCAGC CCACTGTAAT GACTATAAAT CCATCTGGGC AGCAAATCTT GACGAAAACC 
ATGGGTGGCT ACCAACTGCA TCAACCTGGG CCGGAGGCAG CAAGCGCTGG AACGCAGGCT 
GGGCTTGGAA GCTCTGTTTT GAGTTCAGGA GGTGGACTTG TAATTCAAGG GGGCAAGGCC 
ACTCTCGGGT CCCCTGCCTT AAATGGGCCA GCTGTCTGCT TAGGCAGCAC AAATACCAAC 
AACAGTGCAA CTACAATGGC CACTGCTGGT GGTATAGTGG GTTTTAGTAA TGCCTCTTTG 
GGTGCAGGAA TTGGATCTCA GACCCAGCCT CAAGGCCAAA TCATGCAGAA TGTCATCATC 
CAGCGAACGC CAACGCCAAT ACAGCCCAAA CCTCCTCAAG GGGGTACAAT CCAACCCAAA 
ATCTTCAAGC AACAACAACA ACAGCTTCCT GCCCCACATG CCTTGCCAAA TGATGCTAAT 
AAAGCTCTGG GGGTACAGCA AATCCCAGTG TCAGCTGGTC AGAATGTAAC GTTCCTCACC 
GGGAAGCCTG GCTCTAACGT AGTTTTGAGC ACACAGGCAG CGTCGCAGGG AACACAGTTT 
TCTCAAGCGC TCTTTAAACA GCAGGGCCCT CAAACTTCAG GCAAACCCCT CAGTGTTCAC 
TTGTTAAACC AACAGGGCAG CATTGTCATT CCCTCGCAAA CTGTCCTGCA GGGCCAAAAC 
CACCAGTTCC TCTTACCAGG GCTTCAAGCA GGAGGCCAGA TCCTGGCTCA GCATCCTGGT 
GGACACATCA TAACCAGTCA GGGGCCTGGA GGGCAGATAA TCGCTAATCA GATCTTGACA 
GCCAATCAGA ACATCAACTT GGGACAGGTG TTGGCTTCAC AGGGCCACCC TGGTGCAGCC 
CACATTCTCT CTGGACACAT CCAGCTCCAG CCTGGTCAGA TGGGTCAGCC TGCACTGTTC 
CAAATGCCGG TGTCTTTGGC GCATACTCAG ACACAATCTC ATCCGGTAAC CGGGCATGTA 
CAAACGGTCA TTCAGGGCAT GCCTATACAG AATTCCCTGT CTGTGGAGAG CCTTAGCCCA 
GCAGTCAGTT TACAGACGTT GCAGCAGTCT GGCGGCGTCC CTAATAATGG TAGCAGTGGA 
TCGACGGCAA TGGCGCCATG CCAGCCAGGA GAGGGTATCA CTGTATTGGG CGGCTCTTCT 
GACTCTGCTG CTCAACCAGC ACAGACGCAA CCTCAGCCCT CAATTCTCAC GGTTCAGACG 
ACGCCTCCAG TTTCAGTAGC AGCTTCGGTT CCTTCCTCTT CATCTCCATC CCCAACGATG 
GCCACGTCTG CATCCACAAT GGTGGGTCTG TGTTCACAGG CCCAGCACAG CCCTGGAAAA 
GTGTTGTTCA CGTCACCTGG TTCCGGCATG ATCCTGAGCC AGGAGCCTCT TCAAATGTTC 
CTGCACCAGG AGCAACAGCA TCACGCAGGA AAAGACCCAC CTGCCGCTGT GGGTGTACCT 
GCATCTGTTA TCGTCAGCGG CAGCAGTTCT GGTCCCGTCC CTTCAGGCCA TGACAACTTG 
TTAGCTGAGA CTCGGCAGAG GCAGAGTCCC AGTCCCTCCC TTGGTCCTGC CCACATGGCA 
ACAGTGGTTA ACAAGGTTCC ATCGTCAGCA CATTCACAGT CTATAAAGAT CCAAAACGCC 
TCCCAATCTC AGCCTGTGGT CACCCCTTCG CCAGCACCCA CACTGTCCGA CAGCCCTCAA 
CCTGCTCAAG TTTCCCCTCT CACTCTCGGG CAGCAGATCC AGTCTCCCCA TCAACAGTCT 
CGGCCTCCCT CGCAGCCCCA GCCTCCTTCA CAGGCACAGA CTCCTTCACG CTCATGCACC 
CCTTCCTCTC TACCGCCTCT CTTCATCATT CACAATCAGA TGGGAGGTTC TCCACAGCCG 
GCTCCACCAC CTCAGCAACA GCAGCCTCAG CAGTTACAAG TGCAGCTCCA GCCTCAAGTT 
CTTCCTCAGC CTGCTGCTCT GCAGCCAGAC GTACCTCCTT CCTCGTGCTC CCCAAAGCCT 
CCGCAGCCAC TTCCCACACA GTTCCAGTTC CAGCCCCCAG TCTCCAGCTC GTCTCCAGCT 
GCAGCAGTGA AACAGCAGGT GACGGTGGTG CCTGGGCTGA CCGCAGAACA GCAGCATCAC 
CTACAGCTGG TCAGTGCGCA GCTGCAGACC ATGTCATCAA TCACACAGCC CTCCCCTCAA 
CAAAAGCAGC TCTTGGAAAA ACTTCACCAG GTCCAGCAAA ACATACTTTT GCAGGCTAAG 
CAGCAAGCTC AGGCACAAGC CCAGGCCTCT CAGCAACAGG CTACAAACCA GTTCAACAAA 
ATGCCAGATC AGCCTTCGGT CCAAGCTGCG ACTTCTTCAA CGGCCACTGG AGCCATCCAA 
GCACCAGTCC AGTCTCTTCT GCCGCAGAAG TCTGTGCTTG TCAAGCCCTC TACTACAGGT 
GCAAATGACA CACAAGTGTT TTCTGCGGTG TCTGCTGGGG CTACGGTGAA CACGGGAATC 
ACAACACCAA ACCTTGCACA GGCTGTCCAG GCAAAGCCAG GCGTCATAAG TTCGGTTAGT 
GGACTAACTC TGGGCAAAGC AGGTTTGCAG ATTCAGGTGT TAGGTGCTGG ACTCTCTCAA 
ATGCCTGCAC CTCCACTACC TGCTCCCCAA ACACAGACAT CAACATTAAA AAGGCCCTTT 
AGTATGGAAC CAAGCAAAGA AGCTAGGATG CTGGAACAGC TGCGAAAACA GCAAGGCTCA 
GTTCTCCATC CTGACTACAG CTCTCCTTTC CGCTCTTTTG AGGACACACT TCATCGGCTG 
CTGCCATACC ATCTGTACCA AGGCACGGCT TCATCTCCGG AAGACTGTCG TAAAGTGGAT 
GATGAATTTG AGAACGTCTC AAGCCAGCTC TTGAAACGCA CACAAGCAAT GCTGGATAAA 
TATCGTCACT TGCTTTTCGA GGAATCAAAG CAGAGGCTGG GTCCCTCAGC AGAGATGGTT 
ATGATTGACC GGATGTTCAT TCAAGAGGAA AAGGTTGCTC TGAGTCAGGA CAGGGTCCTA 
GCCAAGGAGA AACCAGAGGA ATTTGTGGCC AATTCGTGTT TAGTGGACAG CAGTGCGGTG 
AAGCTTGAAA AGGTGGAGCT AAGTTCTGTA AGAACTGCAT CAACAATGGT TGCGGCTCCG 
CCTACAGCAG TTACGCCTGC TCCTGCCACA ACTGTTGCCC CGGCTCCAAC CTCTACACCT 
GCTCCAGCTG CTGCCCCAAC TCCTGCTCCT GCTCCTGCCC CTGCACTCTT CCCTCCAACA 
AAACTGGTGA TCAAGCAAGG TGGAGGAGGA GCTTCAGTCT CCTGGTCCAC CAGCTCAACC 
CCTACGCCTG CTCCGGTGGT GCGACCGCCT GCTGAGCCTG TCGCACCAAG TGCCTCCTTC 
AGCCGCACTC CGTCCTCCCG CCCTGCTGAC GACGACGATG ACGTTGCACT TCCCCAACGG 
ACCAGCAAAC CACCCATCAA GACCTACGAG GCACGTCGGC GGATAGGTTT GAAGCTGAAG 
ATCAAGCAAG AGGCGGGGTT AAGTAAGGTG GTTCACAACA CTGCATTAGA TCCTGTCCAT 
TCGCAATCAC AACTCACACC ACAGACGCCC ACCCCCGAGC AGCCGCAGAA AGTAAAGCCA 
CACGTAACTC TTCCCTCCAC CGTCATTAGA ACTCAACCCA CAACCTCTCA ATCCACCTCC 
TCCTCCACTG TCACCACAGT AACCACACAC ACAGCCTCCG CCTCCAGTAG CGCAACTTCC 
TCGAGCGGGG CATCGTCTTC ATTCTCCACC TGGTCTTCAT CGTCACCTTC TACGTCGACC 
GCGCAGATGA ACGGCACCCT GGAGCACCAT GAGGTAGGTG GGGTCAAACG TAACCCAGCG 
TCCACAGCAA CGCCACCACT AACAACGTGC AGGCTCCCGC TCCGAAAGAC ATACCGTGAG 
AACATCAGTC CCCGTCACAG GCCTGGAGTA CCAGGAGGTG GTGGAGATAC ACTACCCATT 
CTGCCTGCAG GACCTCCTGT TACCTCTTCG CCCCAACCGC AAAGCTCGTC CCCTCAACCC 
GAACGAACTG TGATAGCCAG TGTGAAACTG GAGCGGCAAG GTGGACACGG GCGATCTCAC 
CCCCATGCGG AGTCACAAAG CCTGGCAGCC GTTGAAGACG TCCTTTATCG GGGAATAAAA 
AACGCGTACC AACATCATCG GGAGTTTGAT AAAGACGATG AGGATGATAT GGAGGAAGGT 
GGTGGTGGTG GTGGTGCTTT GGGGCGGTTA AAGGGCATAG GAAGCAAACA CCGGGAAGGG 
GGACGGGGCG CCTTCAGGAT GGATCAGCAT GCCCCAGGGC CGCCCTCTCC CGGAGAGTCT 
TCCTGCACAA GAGACTCGTC ACTTCCTGCC AAACGCTGCA AGTCGGACTC TCCGGACATG 
GACAATGCAA GCTTCTCCAG CGGCAGCCCG CCGCCTGACG ACTCGCTAAA TGAGCACCTG 
CAGTGTGCCA TAGACAGTAT ACTAAACCTC CAACAGGGCC CTCCTGGACA CGGAGGCTCA 
GGGAGAGGGG ATTTAGGGAG GGTTCATGGG GCAAACCTGC CTAACCAGCA CCAAACCCAT 
CCCTCCTACA GACAGTCCAT GCCTCCCCTC TCAACACAGC CCTCGTCCGC CCCCATGTCC 
CAGCATTCAC AGGTAGGAGG GCGAGGGCAG AATGGAAATC TGGTTTCACA GACGCACAGT 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bicra ( XM_001922789.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001922824.3 Danio rerio BRD4 interacting chromatin remodeling complex associated protein (bicra), transcript variant X3, mRNA.

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CloneID	ODa23560
Clone ID Related Accession (Same CDS sequence)	XM_001922789.6 , XM_001922789.7
Accession Version	XM_001922789.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	glioma tumor suppressor candidate region gene 1 protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001922789.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATG AAGATGGCAG GTGCTTGCTA GATGTAATTT GTGACCCACA AGCACTCAAC 
GATTTTCTTC ATGGATCAGA AACACAAGGC CATGTTCCTG AGGTTCCGGC ACAGGTTCAG 
CTGTCGACAA GTGAGCCGGG CCTTCCAAGA GTCAGCGTTG ATTTGGACTT CTTGGAGGAC 
GATGACATAT TGGGGGGATC GCCAGGAGGA GACAATGGAA GTAATGGTGT TGGGACAAAT 
CATGAGCCCT GTGACATTTT GCAGCAGAGC TTGGCGGAGG CTAACATCAC AGAGCAGAGC 
CTTCAAGAGG CAGATGCTGA GCTTGACCTC AGTTCCTTTG GGCTAACTGG CCTTACGCAG 
GTGGTTCAAC CCCTGCCTGA TGCCGGCCTG TCTGGGGTGG GCATTGGAGG TGCAACTCAG 
ATTTTTCCAA ACCAAGGCAC CCCTACCGCA CCCTCAAATC CCACTCCAGA CATGCTTGGT 
TCTGTCCTGG CACATCCAGG TCTGCAGCTT CAACAACAGG TCATGAACAA GGCTATCAGC 
GTCCAGCCTT TTGTACAACA AGTCGGACTT GGAAATGTAA CACTTCAGCC AATTTCGAGC 
CTTCAGGCCC TACCGAACGG CAGCCAGTCT GGACCGTTGG GCATTGGACA AATTCAAGTG 
GTGGGTCAGC CCACTGTAAT GACTATAAAT CCATCTGGGC AGCAAATCTT GACGAAAACC 
ATGGGTGGCT ACCAACTGCA TCAACCTGGG CCGGAGGCAG CAAGCGCTGG AACGCAGGCT 
GGGCTTGGAA GCTCTGTTTT GAGTTCAGGA GGTGGACTTG TAATTCAAGG GGGCAAGGCC 
ACTCTCGGGT CCCCTGCCTT AAATGGGCCA GCTGTCTGCT TAGGCAGCAC AAATACCAAC 
AACAGTGCAA CTACAATGGC CACTGCTGGT GGTATAGTGG GTTTTAGTAA TGCCTCTTTG 
GGTGCAGGAA TTGGATCTCA GACCCAGCCT CAAGGCCAAA TCATGCAGAA TGTCATCATC 
CAGCGAACGC CAACGCCAAT ACAGCCCAAA CCTCCTCAAG GGGGTACAAT CCAACCCAAA 
ATCTTCAAGC AACAACAACA ACAGCTTCCT GCCCCACATG CCTTGCCAAA TGATGCTAAT 
AAAGCTCTGG GGGTACAGCA AATCCCAGTG TCAGCTGGTC AGAATGTAAC GTTCCTCACC 
GGGAAGCCTG GCTCTAACGT AGTTTTGAGC ACACAGGCAG CGTCGCAGGG AACACAGTTT 
TCTCAAGCGC TCTTTAAACA GCAGGGCCCT CAAACTTCAG GCAAACCCCT CAGTGTTCAC 
TTGTTAAACC AACAGGGCAG CATTGTCATT CCCTCGCAAA CTGTCCTGCA GGGCCAAAAC 
CACCAGTTCC TCTTACCAGG GCTTCAAGCA GGAGGCCAGA TCCTGGCTCA GCATCCTGGT 
GGACACATCA TAACCAGTCA GGGGCCTGGA GGGCAGATAA TCGCTAATCA GATCTTGACA 
GCCAATCAGA ACATCAACTT GGGACAGGTG TTGGCTTCAC AGGGCCACCC TGGTGCAGCC 
CACATTCTCT CTGGACACAT CCAGCTCCAG CCTGGTCAGA TGGGTCAGCC TGCACTGTTC 
CAAATGCCGG TGTCTTTGGC GCATACTCAG ACACAATCTC ATCCGGTAAC CGGGCATGTA 
CAAACGGTCA TTCAGGGCAT GCCTATACAG AATTCCCTGT CTGTGGAGAG CCTTAGCCCA 
GCAGTCAGTT TACAGACGTT GCAGCAGTCT GGCGGCGTCC CTAATAATGG TAGCAGTGGA 
TCGACGGCAA TGGCGCCATG CCAGCCAGGA GAGGGTATCA CTGTATTGGG CGGCTCTTCT 
GACTCTGCTG CTCAACCAGC ACAGACGCAA CCTCAGCCCT CAATTCTCAC GGTTCAGACG 
ACGCCTCCAG TTTCAGTAGC AGCTTCGGTT CCTTCCTCTT CATCTCCATC CCCAACGATG 
GCCACGTCTG CATCCACAAT GGTGGGTCTG TGTTCACAGG CCCAGCACAG CCCTGGAAAA 
GTGTTGTTCA CGTCACCTGG TTCCGGCATG ATCCTGAGCC AGGAGCCTCT TCAAATGTTC 
CTGCACCAGG AGCAACAGCA TCACGCAGGA AAAGACCCAC CTGCCGCTGT GGGTGTACCT 
GCATCTGTTA TCGTCAGCGG CAGCAGTTCT GGTCCCGTCC CTTCAGGCCA TGACAACTTG 
TTAGCTGAGA CTCGGCAGAG GCAGAGTCCC AGTCCCTCCC TTGGTCCTGC CCACATGGCA 
ACAGTGGTTA ACAAGGTTCC ATCGTCAGCA CATTCACAGT CTATAAAGAT CCAAAACGCC 
TCCCAATCTC AGCCTGTGGT CACCCCTTCG CCAGCACCCA CACTGTCCGA CAGCCCTCAA 
CCTGCTCAAG TTTCCCCTCT CACTCTCGGG CAGCAGATCC AGTCTCCCCA TCAACAGTCT 
CGGCCTCCCT CGCAGCCCCA GCCTCCTTCA CAGGCACAGA CTCCTTCACG CTCATGCACC 
CCTTCCTCTC TACCGCCTCT CTTCATCATT CACAATCAGA TGGGAGGTTC TCCACAGCCG 
GCTCCACCAC CTCAGCAACA GCAGCCTCAG CAGTTACAAG TGCAGCTCCA GCCTCAAGTT 
CTTCCTCAGC CTGCTGCTCT GCAGCCAGAC GTACCTCCTT CCTCGTGCTC CCCAAAGCCT 
CCGCAGCCAC TTCCCACACA GTTCCAGTTC CAGCCCCCAG TCTCCAGCTC GTCTCCAGCT 
GCAGCAGTGA AACAGCAGGT GACGGTGGTG CCTGGGCTGA CCGCAGAACA GCAGCATCAC 
CTACAGCTGG TCAGTGCGCA GCTGCAGACC ATGTCATCAA TCACACAGCC CTCCCCTCAA 
CAAAAGCAGC TCTTGGAAAA ACTTCACCAG GTCCAGCAAA ACATACTTTT GCAGGCTAAG 
CAGCAAGCTC AGGCACAAGC CCAGGCCTCT CAGCAACAGG CTACAAACCA GTTCAACAAA 
ATGCCAGATC AGCCTTCGGT CCAAGCTGCG ACTTCTTCAA CGGCCACTGG AGCCATCCAA 
GCACCAGTCC AGTCTCTTCT GCCGCAGAAG TCTGTGCTTG TCAAGCCCTC TACTACAGGT 
GCAAATGACA CACAAGTGTT TTCTGCGGTG TCTGCTGGGG CTACGGTGAA CACGGGAATC 
ACAACACCAA ACCTTGCACA GGCTGTCCAG GCAAAGCCAG GCGTCATAAG TTCGGTTAGT 
GGACTAACTC TGGGCAAAGC AGGTTTGCAG ATTCAGGTGT TAGGTGCTGG ACTCTCTCAA 
ATGCCTGCAC CTCCACTACC TGCTCCCCAA ACACAGACAT CAACATTAAA AAGGCCCTTT 
AGTATGGAAC CAAGCAAAGA AGCTAGGATG CTGGAACAGC TGCGAAAACA GCAAGGCTCA 
GTTCTCCATC CTGACTACAG CTCTCCTTTC CGCTCTTTTG AGGACACACT TCATCGGCTG 
CTGCCATACC ATCTGTACCA AGGCACGGCT TCATCTCCGG AAGACTGTCG TAAAGTGGAT 
GATGAATTTG AGAACGTCTC AAGCCAGCTC TTGAAACGCA CACAAGCAAT GCTGGATAAA 
TATCGTCACT TGCTTTTCGA GGAATCAAAG CAGAGGCTGG GTCCCTCAGC AGAGATGGTT 
ATGATTGACC GGATGTTCAT TCAAGAGGAA AAGGTTGCTC TGAGTCAGGA CAGGGTCCTA 
GCCAAGGAGA AACCAGAGGA ATTTGTGGCC AATTCGTGTT TAGTGGACAG CAGTGCGGTG 
AAGCTTGAAA AGGTGGAGCT AAGTTCTGTA AGAACTGCAT CAACAATGGT TGCGGCTCCG 
CCTACAGCAG TTACGCCTGC TCCTGCCACA ACTGTTGCCC CGGCTCCAAC CTCTACACCT 
GCTCCAGCTG CTGCCCCAAC TCCTGCTCCT GCTCCTGCCC CTGCACTCTT CCCTCCAACA 
AAACTGGTGA TCAAGCAAGG TGGAGGAGGA GCTTCAGTCT CCTGGTCCAC CAGCTCAACC 
CCTACGCCTG CTCCGGTGGT GCGACCGCCT GCTGAGCCTG TCGCACCAAG TGCCTCCTTC 
AGCCGCACTC CGTCCTCCCG CCCTGCTGAC GACGACGATG ACGTTGCACT TCCCCAACGG 
ACCAGCAAAC CACCCATCAA GACCTACGAG GCACGTCGGC GGATAGGTTT GAAGCTGAAG 
ATCAAGCAAG AGGCGGGGTT AAGTAAGGTG GTTCACAACA CTGCATTAGA TCCTGTCCAT 
TCGCAATCAC AACTCACACC ACAGACGCCC ACCCCCGAGC AGCCGCAGAA AGTAAAGCCA 
CACGTAACTC TTCCCTCCAC CGTCATTAGA ACTCAACCCA CAACCTCTCA ATCCACCTCC 
TCCTCCACTG TCACCACAGT AACCACACAC ACAGCCTCCG CCTCCAGTAG CGCAACTTCC 
TCGAGCGGGG CATCGTCTTC ATTCTCCACC TGGTCTTCAT CGTCACCTTC TACGTCGACC 
GCGCAGATGA ACGGCACCCT GGAGCACCAT GAGGTAGGTG GGGTCAAACG TAACCCAGCG 
TCCACAGCAA CGCCACCACT AACAACGTGC AGGCTCCCGC TCCGAAAGAC ATACCGTGAG 
AACATCAGTC CCCGTCACAG GCCTGGAGTA CCAGGAGGTG GTGGAGATAC ACTACCCATT 
CTGCCTGCAG GACCTCCTGT TACCTCTTCG CCCCAACCGC AAAGCTCGTC CCCTCAACCC 
GAACGAACTG TGATAGCCAG TGTGAAACTG GAGCGGCAAG GTGGACACGG GCGATCTCAC 
CCCCATGCGG AGTCACAAAG CCTGGCAGCC GTTGAAGACG TCCTTTATCG GGGAATAAAA 
AACGCGTACC AACATCATCG GGAGTTTGAT AAAGACGATG AGGATGATAT GGAGGAAGGT 
GGTGGTGGTG GTGGTGCTTT GGGGCGGTTA AAGGGCATAG GAAGCAAACA CCGGGAAGGG 
GGACGGGGCG CCTTCAGGAT GGATCAGCAT GCCCCAGGGC CGCCCTCTCC CGGAGAGTCT 
TCCTGCACAA GAGACTCGTC ACTTCCTGCC AAACGCTGCA AGTCGGACTC TCCGGACATG 
GACAATGCAA GCTTCTCCAG CGGCAGCCCG CCGCCTGACG ACTCGCTAAA TGAGCACCTG 
CAGTGTGCCA TAGACAGTAT ACTAAACCTC CAACAGGGCC CTCCTGGACA CGGAGGCTCA 
GGGAGAGGGG ATTTAGGGAG GGTTCATGGG GCAAACCTGC CTAACCAGCA CCAAACCCAT 
CCCTCCTACA GACAGTCCAT GCCTCCCCTC TCAACACAGC CCTCGTCCGC CCCCATGTCC 
CAGCATTCAC AGGTAGGAGG GCGAGGGCAG AATGGAAATC TGGTTTCACA GACGCACAGT 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001922824.3
CDS	951..6356
Translation	MDDEDGRCLLDVICDPQALNDFLHGSETQGHVPEVPAQVQLSTSEPGLPRVSVDLDFLEDDDILGGSPGGDNGSNGVGTNHEPCDILQQSLAEANITEQSLQEADAELDLSSFGLTGLTQVVQPLPDAGLSGVGIGGATQIFPNQGTPTAPSNPTPDMLGSVLAHPGLQLQQQVMNKAISVQPFVQQVGLGNVTLQPISSLQALPNGSQSGPLGIGQIQVVGQPTVMTINPSGQQILTKTMGGYQLHQPGPEAASAGTQAGLGSSVLSSGGGLVIQGGKATLGSPALNGPAVCLGSTNTNNSATTMATAGGIVGFSNASLGAGIGSQTQPQGQIMQNVIIQRTPTPIQPKPPQGGTIQPKIFKQQQQQLPAPHALPNDANKALGVQQIPVSAGQNVTFLTGKPGSNVVLSTQAASQGTQFSQALFKQQGPQTSGKPLSVHLLNQQGSIVIPSQTVLQGQNHQFLLPGLQAGGQILAQHPGGHIITSQGPGGQIIANQILTANQNINLGQVLASQGHPGAAHILSGHIQLQPGQMGQPALFQMPVSLAHTQTQSHPVTGHVQTVIQGMPIQNSLSVESLSPAVSLQTLQQSGGVPNNGSSGSTAMAPCQPGEGITVLGGSSDSAAQPAQTQPQPSILTVQTTPPVSVAASVPSSSSPSPTMATSASTMVGLCSQAQHSPGKVLFTSPGSGMILSQEPLQMFLHQEQQHHAGKDPPAAVGVPASVIVSGSSSGPVPSGHDNLLAETRQRQSPSPSLGPAHMATVVNKVPSSAHSQSIKIQNASQSQPVVTPSPAPTLSDSPQPAQVSPLTLGQQIQSPHQQSRPPSQPQPPSQAQTPSRSCTPSSLPPLFIIHNQMGGSPQPAPPPQQQQPQQLQVQLQPQVLPQPAALQPDVPPSSCSPKPPQPLPTQFQFQPPVSSSSPAAAVKQQVTVVPGLTAEQQHHLQLVSAQLQTMSSITQPSPQQKQLLEKLHQVQQNILLQAKQQAQAQAQASQQQATNQFNKMPDQPSVQAATSSTATGAIQAPVQSLLPQKSVLVKPSTTGANDTQVFSAVSAGATVNTGITTPNLAQAVQAKPGVISSVSGLTLGKAGLQIQVLGAGLSQMPAPPLPAPQTQTSTLKRPFSMEPSKEARMLEQLRKQQGSVLHPDYSSPFRSFEDTLHRLLPYHLYQGTASSPEDCRKVDDEFENVSSQLLKRTQAMLDKYRHLLFEESKQRLGPSAEMVMIDRMFIQEEKVALSQDRVLAKEKPEEFVANSCLVDSSAVKLEKVELSSVRTASTMVAAPPTAVTPAPATTVAPAPTSTPAPAAAPTPAPAPAPALFPPTKLVIKQGGGGASVSWSTSSTPTPAPVVRPPAEPVAPSASFSRTPSSRPADDDDDVALPQRTSKPPIKTYEARRRIGLKLKIKQEAGLSKVVHNTALDPVHSQSQLTPQTPTPEQPQKVKPHVTLPSTVIRTQPTTSQSTSSSTVTTVTTHTASASSSATSSSGASSSFSTWSSSSPSTSTAQMNGTLEHHEVGGVKRNPASTATPPLTTCRLPLRKTYRENISPRHRPGVPGGGGDTLPILPAGPPVTSSPQPQSSSPQPERTVIASVKLERQGGHGRSHPHAESQSLAAVEDVLYRGIKNAYQHHREFDKDDEDDMEEGGGGGGALGRLKGIGSKHREGGRGAFRMDQHAPGPPSPGESSCTRDSSLPAKRCKSDSPDMDNASFSSGSPPPDDSLNEHLQCAIDSILNLQQGPPGHGGSGRGDLGRVHGANLPNQHQTHPSYRQSMPPLSTQPSSAPMSQHSQVGGRGQNGNLVSQTHSR

Target ORF information:

RefSeq Version	XM_001922789.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio BRD4 interacting chromatin remodeling complex associated protein (bicra), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001922789.7

ORF Insert Sequence:

ATGGATGATG AAGATGGCAG GTGCTTGCTA GATGTAATTT GTGACCCACA AGCACTCAAC 
GATTTTCTTC ATGGATCAGA AACACAAGGC CATGTTCCTG AGGTTCCGGC ACAGGTTCAG 
CTGTCGACAA GTGAGCCGGG CCTTCCAAGA GTCAGCGTTG ATTTGGACTT CTTGGAGGAC 
GATGACATAT TGGGGGGATC GCCAGGAGGA GACAATGGAA GTAATGGTGT TGGGACAAAT 
CATGAGCCCT GTGACATTTT GCAGCAGAGC TTGGCGGAGG CTAACATCAC AGAGCAGAGC 
CTTCAAGAGG CAGATGCTGA GCTTGACCTC AGTTCCTTTG GGCTAACTGG CCTTACGCAG 
GTGGTTCAAC CCCTGCCTGA TGCCGGCCTG TCTGGGGTGG GCATTGGAGG TGCAACTCAG 
ATTTTTCCAA ACCAAGGCAC CCCTACCGCA CCCTCAAATC CCACTCCAGA CATGCTTGGT 
TCTGTCCTGG CACATCCAGG TCTGCAGCTT CAACAACAGG TCATGAACAA GGCTATCAGC 
GTCCAGCCTT TTGTACAACA AGTCGGACTT GGAAATGTAA CACTTCAGCC AATTTCGAGC 
CTTCAGGCCC TACCGAACGG CAGCCAGTCT GGACCGTTGG GCATTGGACA AATTCAAGTG 
GTGGGTCAGC CCACTGTAAT GACTATAAAT CCATCTGGGC AGCAAATCTT GACGAAAACC 
ATGGGTGGCT ACCAACTGCA TCAACCTGGG CCGGAGGCAG CAAGCGCTGG AACGCAGGCT 
GGGCTTGGAA GCTCTGTTTT GAGTTCAGGA GGTGGACTTG TAATTCAAGG GGGCAAGGCC 
ACTCTCGGGT CCCCTGCCTT AAATGGGCCA GCTGTCTGCT TAGGCAGCAC AAATACCAAC 
AACAGTGCAA CTACAATGGC CACTGCTGGT GGTATAGTGG GTTTTAGTAA TGCCTCTTTG 
GGTGCAGGAA TTGGATCTCA GACCCAGCCT CAAGGCCAAA TCATGCAGAA TGTCATCATC 
CAGCGAACGC CAACGCCAAT ACAGCCCAAA CCTCCTCAAG GGGGTACAAT CCAACCCAAA 
ATCTTCAAGC AACAACAACA ACAGCTTCCT GCCCCACATG CCTTGCCAAA TGATGCTAAT 
AAAGCTCTGG GGGTACAGCA AATCCCAGTG TCAGCTGGTC AGAATGTAAC GTTCCTCACC 
GGGAAGCCTG GCTCTAACGT AGTTTTGAGC ACACAGGCAG CGTCGCAGGG AACACAGTTT 
TCTCAAGCGC TCTTTAAACA GCAGGGCCCT CAAACTTCAG GCAAACCCCT CAGTGTTCAC 
TTGTTAAACC AACAGGGCAG CATTGTCATT CCCTCGCAAA CTGTCCTGCA GGGCCAAAAC 
CACCAGTTCC TCTTACCAGG GCTTCAAGCA GGAGGCCAGA TCCTGGCTCA GCATCCTGGT 
GGACACATCA TAACCAGTCA GGGGCCTGGA GGGCAGATAA TCGCTAATCA GATCTTGACA 
GCCAATCAGA ACATCAACTT GGGACAGGTG TTGGCTTCAC AGGGCCACCC TGGTGCAGCC 
CACATTCTCT CTGGACACAT CCAGCTCCAG CCTGGTCAGA TGGGTCAGCC TGCACTGTTC 
CAAATGCCGG TGTCTTTGGC GCATACTCAG ACACAATCTC ATCCGGTAAC CGGGCATGTA 
CAAACGGTCA TTCAGGGCAT GCCTATACAG AATTCCCTGT CTGTGGAGAG CCTTAGCCCA 
GCAGTCAGTT TACAGACGTT GCAGCAGTCT GGCGGCGTCC CTAATAATGG TAGCAGTGGA 
TCGACGGCAA TGGCGCCATG CCAGCCAGGA GAGGGTATCA CTGTATTGGG CGGCTCTTCT 
GACTCTGCTG CTCAACCAGC ACAGACGCAA CCTCAGCCCT CAATTCTCAC GGTTCAGACG 
ACGCCTCCAG TTTCAGTAGC AGCTTCGGTT CCTTCCTCTT CATCTCCATC CCCAACGATG 
GCCACGTCTG CATCCACAAT GGTGGGTCTG TGTTCACAGG CCCAGCACAG CCCTGGAAAA 
GTGTTGTTCA CGTCACCTGG TTCCGGCATG ATCCTGAGCC AGGAGCCTCT TCAAATGTTC 
CTGCACCAGG AGCAACAGCA TCACGCAGGA AAAGACCCAC CTGCCGCTGT GGGTGTACCT 
GCATCTGTTA TCGTCAGCGG CAGCAGTTCT GGTCCCGTCC CTTCAGGCCA TGACAACTTG 
TTAGCTGAGA CTCGGCAGAG GCAGAGTCCC AGTCCCTCCC TTGGTCCTGC CCACATGGCA 
ACAGTGGTTA ACAAGGTTCC ATCGTCAGCA CATTCACAGT CTATAAAGAT CCAAAACGCC 
TCCCAATCTC AGCCTGTGGT CACCCCTTCG CCAGCACCCA CACTGTCCGA CAGCCCTCAA 
CCTGCTCAAG TTTCCCCTCT CACTCTCGGG CAGCAGATCC AGTCTCCCCA TCAACAGTCT 
CGGCCTCCCT CGCAGCCCCA GCCTCCTTCA CAGGCACAGA CTCCTTCACG CTCATGCACC 
CCTTCCTCTC TACCGCCTCT CTTCATCATT CACAATCAGA TGGGAGGTTC TCCACAGCCG 
GCTCCACCAC CTCAGCAACA GCAGCCTCAG CAGTTACAAG TGCAGCTCCA GCCTCAAGTT 
CTTCCTCAGC CTGCTGCTCT GCAGCCAGAC GTACCTCCTT CCTCGTGCTC CCCAAAGCCT 
CCGCAGCCAC TTCCCACACA GTTCCAGTTC CAGCCCCCAG TCTCCAGCTC GTCTCCAGCT 
GCAGCAGTGA AACAGCAGGT GACGGTGGTG CCTGGGCTGA CCGCAGAACA GCAGCATCAC 
CTACAGCTGG TCAGTGCGCA GCTGCAGACC ATGTCATCAA TCACACAGCC CTCCCCTCAA 
CAAAAGCAGC TCTTGGAAAA ACTTCACCAG GTCCAGCAAA ACATACTTTT GCAGGCTAAG 
CAGCAAGCTC AGGCACAAGC CCAGGCCTCT CAGCAACAGG CTACAAACCA GTTCAACAAA 
ATGCCAGATC AGCCTTCGGT CCAAGCTGCG ACTTCTTCAA CGGCCACTGG AGCCATCCAA 
GCACCAGTCC AGTCTCTTCT GCCGCAGAAG TCTGTGCTTG TCAAGCCCTC TACTACAGGT 
GCAAATGACA CACAAGTGTT TTCTGCGGTG TCTGCTGGGG CTACGGTGAA CACGGGAATC 
ACAACACCAA ACCTTGCACA GGCTGTCCAG GCAAAGCCAG GCGTCATAAG TTCGGTTAGT 
GGACTAACTC TGGGCAAAGC AGGTTTGCAG ATTCAGGTGT TAGGTGCTGG ACTCTCTCAA 
ATGCCTGCAC CTCCACTACC TGCTCCCCAA ACACAGACAT CAACATTAAA AAGGCCCTTT 
AGTATGGAAC CAAGCAAAGA AGCTAGGATG CTGGAACAGC TGCGAAAACA GCAAGGCTCA 
GTTCTCCATC CTGACTACAG CTCTCCTTTC CGCTCTTTTG AGGACACACT TCATCGGCTG 
CTGCCATACC ATCTGTACCA AGGCACGGCT TCATCTCCGG AAGACTGTCG TAAAGTGGAT 
GATGAATTTG AGAACGTCTC AAGCCAGCTC TTGAAACGCA CACAAGCAAT GCTGGATAAA 
TATCGTCACT TGCTTTTCGA GGAATCAAAG CAGAGGCTGG GTCCCTCAGC AGAGATGGTT 
ATGATTGACC GGATGTTCAT TCAAGAGGAA AAGGTTGCTC TGAGTCAGGA CAGGGTCCTA 
GCCAAGGAGA AACCAGAGGA ATTTGTGGCC AATTCGTGTT TAGTGGACAG CAGTGCGGTG 
AAGCTTGAAA AGGTGGAGCT AAGTTCTGTA AGAACTGCAT CAACAATGGT TGCGGCTCCG 
CCTACAGCAG TTACGCCTGC TCCTGCCACA ACTGTTGCCC CGGCTCCAAC CTCTACACCT 
GCTCCAGCTG CTGCCCCAAC TCCTGCTCCT GCTCCTGCCC CTGCACTCTT CCCTCCAACA 
AAACTGGTGA TCAAGCAAGG TGGAGGAGGA GCTTCAGTCT CCTGGTCCAC CAGCTCAACC 
CCTACGCCTG CTCCGGTGGT GCGACCGCCT GCTGAGCCTG TCGCACCAAG TGCCTCCTTC 
AGCCGCACTC CGTCCTCCCG CCCTGCTGAC GACGACGATG ACGTTGCACT TCCCCAACGG 
ACCAGCAAAC CACCCATCAA GACCTACGAG GCACGTCGGC GGATAGGTTT GAAGCTGAAG 
ATCAAGCAAG AGGCGGGGTT AAGTAAGGTG GTTCACAACA CTGCATTAGA TCCTGTCCAT 
TCGCAATCAC AACTCACACC ACAGACGCCC ACCCCCGAGC AGCCGCAGAA AGTAAAGCCA 
CACGTAACTC TTCCCTCCAC CGTCATTAGA ACTCAACCCA CAACCTCTCA ATCCACCTCC 
TCCTCCACTG TCACCACAGT AACCACACAC ACAGCCTCCG CCTCCAGTAG CGCAACTTCC 
TCGAGCGGGG CATCGTCTTC ATTCTCCACC TGGTCTTCAT CGTCACCTTC TACGTCGACC 
GCGCAGATGA ACGGCACCCT GGAGCACCAT GAGGTAGGTG GGGTCAAACG TAACCCAGCG 
TCCACAGCAA CGCCACCACT AACAACGTGC AGGCTCCCGC TCCGAAAGAC ATACCGTGAG 
AACATCAGTC CCCGTCACAG GCCTGGAGTA CCAGGAGGTG GTGGAGATAC ACTACCCATT 
CTGCCTGCAG GACCTCCTGT TACCTCTTCG CCCCAACCGC AAAGCTCGTC CCCTCAACCC 
GAACGAACTG TGATAGCCAG TGTGAAACTG GAGCGGCAAG GTGGACACGG GCGATCTCAC 
CCCCATGCGG AGTCACAAAG CCTGGCAGCC GTTGAAGACG TCCTTTATCG GGGAATAAAA 
AACGCGTACC AACATCATCG GGAGTTTGAT AAAGACGATG AGGATGATAT GGAGGAAGGT 
GGTGGTGGTG GTGGTGCTTT GGGGCGGTTA AAGGGCATAG GAAGCAAACA CCGGGAAGGG 
GGACGGGGCG CCTTCAGGAT GGATCAGCAT GCCCCAGGGC CGCCCTCTCC CGGAGAGTCT 
TCCTGCACAA GAGACTCGTC ACTTCCTGCC AAACGCTGCA AGTCGGACTC TCCGGACATG 
GACAATGCAA GCTTCTCCAG CGGCAGCCCG CCGCCTGACG ACTCGCTAAA TGAGCACCTG 
CAGTGTGCCA TAGACAGTAT ACTAAACCTC CAACAGGGCC CTCCTGGACA CGGAGGCTCA 
GGGAGAGGGG ATTTAGGGAG GGTTCATGGG GCAAACCTGC CTAACCAGCA CCAAACCCAT 
CCCTCCTACA GACAGTCCAT GCCTCCCCTC TCAACACAGC CCTCGTCCGC CCCCATGTCC 
CAGCATTCAC AGGTAGGAGG GCGAGGGCAG AATGGAAATC TGGTTTCACA GACGCACAGT 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bicra ( XM_005159254.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159311.1 Danio rerio glioma tumor suppressor candidate region gene 1 (gltscr1), transcript variant X4, mRNA.

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CloneID	ODa23561
Clone ID Related Accession (Same CDS sequence)	XM_005159254.3 , XM_005159254.4
Accession Version	XM_005159254.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	glioma tumor suppressor candidate region gene 1 protein isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159254.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATG AAGATGGCAG GTGCTTGCTA GATGTAATTT GTGACCCACA AGCACTCAAC 
GATTTTCTTC ATGGATCAGA AACACAACTG GACACTGATG ACCTATTGGA TGGCTCAGGT 
GACCCGTCCA GCTCGTTCTT CTCCAGCACT GGGGGCCATG TTCCTGAGGT TCCGGCACAG 
GTTCAGCTGT CGACAAGTGA GCCGGGCCTT CCAAGAGTCA GCGTTGATTT GGACTTCTTG 
GAGGACGATG ACATATTGGG GGGATCGCCA GGAGGAGACA ATGGAAGTAA TGGTGTTGGG 
ACAAATCATG AGCCCTGTGA CATTTTGCAG CAGAGCTTGG CGGAGGCTAA CATCACAGAG 
CAGAGCCTTC AAGAGGCAGA TGCTGAGCTT GACCTCAGTT CCTTTGGGCT AACTGGCCTT 
ACGCAGGTGG TTCAACCCCT GCCTGATGCC GGCCTGTCTG GGGTGGGCAT TGGAGGTGCA 
ACTCAGATTT TTCCAAACCA AGGCACCCCT ACCGCACCCT CAAATCCCAC TCCAGACATG 
CTTGGTTCTG TCCTGGCACA TCCAGGTCTG CAGCTTCAAC AACAGGTCAT GAACAAGGCT 
ATCAGCGTCC AGCCTTTTGT ACAACAAGTC GGACTTGGAA ATGTAACACT TCAGCCAATT 
TCGAGCCTTC AGGCCCTACC GAACGGCAGC CAGTCTGGAC CGTTGGGCAT TGGACAAATT 
CAAGTGGTGG GTCAGCCCAC TGTAATGACT ATAAATCCAT CTGGGCAGCA AATCTTGACG 
AAAACCATGG GTGGCTACCA ACTGCATCAA CCTGGGCCGG AGGCAGCAAG CGCTGGAACG 
CAGGCTGGGC TTGGAAGCTC TGTTTTGAGT TCAGGAGGTG GACTTGTAAT TCAAGGGGGC 
AAGGCCACTC TCGGGTCCCC TGCCTTAAAT GGGCCAGCTG TCTGCTTAGG CAGCACAAAT 
ACCAACAACA GTGCAACTAC AATGGCCACT GCTGGTGGTA TAGTGGGTTT TAGTAATGCC 
TCTTTGGGTG CAGGAATTGG ATCTCAGACC CAGCCTCAAG GCCAAATCAT GCAGAATGTC 
ATCATCCAGC GAACGCCAAC GCCAATACAG CCCAAACCTC CTCAAGGGGG TACAATCCAA 
CCCAAAATCT TCAAGCAACA ACAACAACAG CTTCCTGCCC CACATGCCTT GCCAAATGAT 
GCTAATAAAG CTCTGGGGGT ACAGCAAATC CCAGTGTCAG CTGGTCAGAA TGTAACGTTC 
CTCACCGGGA AGCCTGGCTC TAACGTAGTT TTGAGCACAC AGGCAGCGTC GCAGGGAACA 
CAGTTTTCTC AAGCGCTCTT TAAACAGCAG GGCCCTCAAA CTTCAGGCAA ACCCCTCAGT 
GTTCACTTGT TAAACCAACA GGGCAGCATT GTCATTCCCT CGCAAACTGT CCTGCAGGGC 
CAAAACCACC AGTTCCTCTT ACCAGGGCTT CAAGCAGGAG GCCAGATCCT GGCTCAGCAT 
CCTGGTGGAC ACATCATAAC CAGTCAGGGG CCTGGAGGGC AGATAATCGC TAATCAGATC 
TTGACAGCCA ATCAGAACAT CAACTTGGGA CAGGTGTTGG CTTCACAGGG CCACCCTGGT 
GCAGCCCACA TTCTCTCTGG ACACATCCAG CTCCAGCCTG GTCAGATGGG TCAGCCTGCA 
CTGTTCCAAA TGCCGGTGTC TTTGGCGCAT ACTCAGACAC AATCTCATCC GGTAACCGGG 
CATGTACAAA CGGTCATTCA GGGCATGCCT ATACAGAATT CCCTGTCTGT GGAGAGCCTT 
AGCCCAGCAG TCAGTTTACA GACGTTGCAG CAGTCTGGCG GCGTCCCTAA TAATGGTAGC 
AGTGGATCGA CGGCAATGGC GCCATGCCAG CCAGGAGAGG GTATCACTGT ATTGGGCGGC 
TCTTCTGACT CTGCTGCTCA ACCAGCACAG ACGCAACCTC AGCCCTCAAT TCTCACGGTT 
CAGACGACGC CTCCAGTTTC AGTAGCAGCT TCGGTTCCTT CCTCTTCATC TCCATCCCCA 
ACGATGGCCA CGTCTGCATC CACAATGGTG GGTCTGTGTT CACAGGCCCA GCACAGCCCT 
GGAAAAGTGT TGTTCACGTC ACCTGGTTCC GGCATGATCC TGAGCCAGGA GCCTCTTCAA 
ATGTTCCTGC ACCAGGTTCC ATCGTCAGCA CATTCACAGT CTATAAAGAT CCAAAACGCC 
TCCCAATCTC AGCCTGTGGT CACCCCTTCG CCAGCACCCA CACTGTCCGA CAGCCCTCAA 
CCTGCTCAAG TTTCCCCTCT CACTCTCGGG CAGCAGATCC AGTCTCCCCA TCAACAGTCT 
CGGCCTCCCT CGCAGCCCCA GCCTCCTTCA CAGGCACAGA CTCCTTCACG CTCATGCACC 
CCTTCCTCTC TACCGCCTCT CTTCATCATT CACAATCAGA TGGGAGGTTC TCCACAGCCG 
GCTCCACCAC CTCAGCAACA GCAGCCTCAG CAGTTACAAG TGCAGCTCCA GCCTCAAGTT 
CTTCCTCAGC CTGCTGCTCT GCAGCCAGAC GTACCTCCTT CCTCGTGCTC CCCAAAGCCT 
CCGCAGCCAC TTCCCACACA GTTCCAGTTC CAGCCCCCAG TCTCCAGCTC GTCTCCAGCT 
GCAGCAGTGA AACAGCAGGT GACGGTGGTG CCTGGGCTGA CCGCAGAACA GCAGCATCAC 
CTACAGCTGG TCAGTGCGCA GCTGCAGACC ATGTCATCAA TCACACAGCC CTCCCCTCAA 
CAAAAGCAGC TCTTGGAAAA ACTTCACCAG GTCCAGCAAA ACATACTTTT GCAGGCTAAG 
CAGCAAGCTC AGGCACAAGC CCAGGCCTCT CAGCAACAGG CTACAAACCA GTTCAACAAA 
ATGCCAGATC AGCCTTCGGT CCAAGCTGCG ACTTCTTCAA CGGCCACTGG AGCCATCCAA 
GCACCAGTCC AGTCTCTTCT GCCGCAGAAG TCTGTGCTTG TCAAGCCCTC TACTACAGGT 
GCAAATGACA CACAAGTGTT TTCTGCGGTG TCTGCTGGGG CTACGGTGAA CACGGGAATC 
ACAACACCAA ACCTTGCACA GGCTGTCCAG GCAAAGCCAG GCGTCATAAG TTCGGTTAGT 
GGACTAACTC TGGGCAAAGC AGGTTTGCAG ATTCAGGTGT TAGGTGCTGG ACTCTCTCAA 
ATGCCTGCAC CTCCACTACC TGCTCCCCAA ACACAGACAT CAACATTAAA AAGGCCCTTT 
AGTATGGAAC CAAGCAAAGA AGCTAGGATG CTGGAACAGC TGCGAAAACA GCAAGGCTCA 
GTTCTCCATC CTGACTACAG CTCTCCTTTC CGCTCTTTTG AGGACACACT TCATCGGCTG 
CTGCCATACC ATCTGTACCA AGGCACGGCT TCATCTCCGG AAGACTGTCG TAAAGTGGAT 
GATGAATTTG AGAACGTCTC AAGCCAGCTC TTGAAACGCA CACAAGCAAT GCTGGATAAA 
TATCGTCACT TGCTTTTCGA GGAATCAAAG CAGAGGCTGG GTCCCTCAGC AGAGATGGTT 
ATGATTGACC GGATGTTCAT TCAAGAGGAA AAGGTTGCTC TGAGTCAGGA CAGGGTCCTA 
GCCAAGGAGA AACCAGAGGA ATTTGTGGCC AATTCGTGTT TAGTGGACAG CAGTGCGGTG 
AAGCTTGAAA AGGTGGAGCT AAGTTCTGTA AGAACTGCAT CAACAATGGT TGCGGCTCCG 
CCTACAGCAG TTACGCCTGC TCCTGCCACA ACTGTTGCCC CGGCTCCAAC CTCTACACCT 
GCTCCAGCTG CTGCCCCAAC TCCTGCTCCT GCTCCTGCCC CTGCACTCTT CCCTCCAACA 
AAACTGGTGA TCAAGCAAGG TGGAGGAGGA GCTTCAGTCT CCTGGTCCAC CAGCTCAACC 
CCTACGCCTG CTCCGGTGGT GCGACCGCCT GCTGAGCCTG TCGCACCAAG TGCCTCCTTC 
AGCCGCACTC CGTCCTCCCG CCCTGCTGAC GACGACGATG ACGTTGCACT TCCCCAACGG 
ACCAGCAAAC CACCCATCAA GACCTACGAG GCACGTCGGC GGATAGGTTT GAAGCTGAAG 
ATCAAGCAAG AGGCGGGGTT AAGTAAGGTG GTTCACAACA CTGCATTAGA TCCTGTCCAT 
TCGCAATCAC AACTCACACC ACAGACGCCC ACCCCCGAGC AGCCGCAGAA AGTAAAGCCA 
CACGTAACTC TTCCCTCCAC CGTCATTAGA ACTCAACCCA CAACCTCTCA ATCCACCTCC 
TCCTCCACTG TCACCACAGT AACCACACAC ACAGCCTCCG CCTCCAGTAG CGCAACTTCC 
TCGAGCGGGG CATCGTCTTC ATTCTCCACC TGGTCTTCAT CGTCACCTTC TACGTCGACC 
GCGCAGATGA ACGGCACCCT GGAGCACCAT GAGGTAGGTG GGGTCAAACG TAACCCAGCG 
TCCACAGCAA CGCCACCACT AACAACGTGC AGGCTCCCGC TCCGAAAGAC ATACCGTGAG 
AACATCAGTC CCCGTCACAG GCCTGGAGTA CCAGGAGGTG GTGGAGATAC ACTACCCATT 
CTGCCTGCAG GACCTCCTGT TACCTCTTCG CCCCAACCGC AAAGCTCGTC CCCTCAACCC 
GAACGAACTG TGATAGCCAG TGTGAAACTG GAGCGGCAAG GTGGACACGG GCGATCTCAC 
CCCCATGCGG AGTCACAAAG CCTGGCAGCC GTTGAAGACG TCCTTTATCG GGGAATAAAA 
AACGCGTACC AACATCATCG GGAGTTTGAT AAAGACGATG AGGATGATAT GGAGGAAGGT 
GGTGGTGGTG GTGGTGCTTT GGGGCGGTTA AAGGGCATAG GAAGCAAACA CCGGGAAGGG 
GGACGGGGCG CCTTCAGGAT GGATCAGCAT GCCCCAGGGC CGCCCTCTCC CGGAGAGTCT 
TCCTGCACAA GAGACTCGTC ACTTCCTGCC AAACGCTGCA AGTCGGACTC TCCGGACATG 
GACAATGCAA GCTTCTCCAG CGGCAGCCCG CCGCCTGACG ACTCGCTAAA TGAGCACCTG 
CAGTGTGCCA TAGACAGTAT ACTAAACCTC CAACAGGGCC CTCCTGGACA CGGAGGCTCA 
GGGAGAGGGG ATTTAGGGAG GGTTCATGGG GCAAACCTGC CTAACCAGCA CCAAACCCAT 
CCCTCCTACA GACAGTCCAT GCCTCCCCTC TCAACACAGC CCTCGTCCGC CCCCATGTCC 
CAGCATTCAC AGGTAGGAGG GCGAGGGCAG AATGGAAATC TGGTTTCACA GACGCACAGT 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159311.1
CDS	1147..6432
Translation	MDDEDGRCLLDVICDPQALNDFLHGSETQLDTDDLLDGSGDPSSSFFSSTGGHVPEVPAQVQLSTSEPGLPRVSVDLDFLEDDDILGGSPGGDNGSNGVGTNHEPCDILQQSLAEANITEQSLQEADAELDLSSFGLTGLTQVVQPLPDAGLSGVGIGGATQIFPNQGTPTAPSNPTPDMLGSVLAHPGLQLQQQVMNKAISVQPFVQQVGLGNVTLQPISSLQALPNGSQSGPLGIGQIQVVGQPTVMTINPSGQQILTKTMGGYQLHQPGPEAASAGTQAGLGSSVLSSGGGLVIQGGKATLGSPALNGPAVCLGSTNTNNSATTMATAGGIVGFSNASLGAGIGSQTQPQGQIMQNVIIQRTPTPIQPKPPQGGTIQPKIFKQQQQQLPAPHALPNDANKALGVQQIPVSAGQNVTFLTGKPGSNVVLSTQAASQGTQFSQALFKQQGPQTSGKPLSVHLLNQQGSIVIPSQTVLQGQNHQFLLPGLQAGGQILAQHPGGHIITSQGPGGQIIANQILTANQNINLGQVLASQGHPGAAHILSGHIQLQPGQMGQPALFQMPVSLAHTQTQSHPVTGHVQTVIQGMPIQNSLSVESLSPAVSLQTLQQSGGVPNNGSSGSTAMAPCQPGEGITVLGGSSDSAAQPAQTQPQPSILTVQTTPPVSVAASVPSSSSPSPTMATSASTMVGLCSQAQHSPGKVLFTSPGSGMILSQEPLQMFLHQVPSSAHSQSIKIQNASQSQPVVTPSPAPTLSDSPQPAQVSPLTLGQQIQSPHQQSRPPSQPQPPSQAQTPSRSCTPSSLPPLFIIHNQMGGSPQPAPPPQQQQPQQLQVQLQPQVLPQPAALQPDVPPSSCSPKPPQPLPTQFQFQPPVSSSSPAAAVKQQVTVVPGLTAEQQHHLQLVSAQLQTMSSITQPSPQQKQLLEKLHQVQQNILLQAKQQAQAQAQASQQQATNQFNKMPDQPSVQAATSSTATGAIQAPVQSLLPQKSVLVKPSTTGANDTQVFSAVSAGATVNTGITTPNLAQAVQAKPGVISSVSGLTLGKAGLQIQVLGAGLSQMPAPPLPAPQTQTSTLKRPFSMEPSKEARMLEQLRKQQGSVLHPDYSSPFRSFEDTLHRLLPYHLYQGTASSPEDCRKVDDEFENVSSQLLKRTQAMLDKYRHLLFEESKQRLGPSAEMVMIDRMFIQEEKVALSQDRVLAKEKPEEFVANSCLVDSSAVKLEKVELSSVRTASTMVAAPPTAVTPAPATTVAPAPTSTPAPAAAPTPAPAPAPALFPPTKLVIKQGGGGASVSWSTSSTPTPAPVVRPPAEPVAPSASFSRTPSSRPADDDDDVALPQRTSKPPIKTYEARRRIGLKLKIKQEAGLSKVVHNTALDPVHSQSQLTPQTPTPEQPQKVKPHVTLPSTVIRTQPTTSQSTSSSTVTTVTTHTASASSSATSSSGASSSFSTWSSSSPSTSTAQMNGTLEHHEVGGVKRNPASTATPPLTTCRLPLRKTYRENISPRHRPGVPGGGGDTLPILPAGPPVTSSPQPQSSSPQPERTVIASVKLERQGGHGRSHPHAESQSLAAVEDVLYRGIKNAYQHHREFDKDDEDDMEEGGGGGGALGRLKGIGSKHREGGRGAFRMDQHAPGPPSPGESSCTRDSSLPAKRCKSDSPDMDNASFSSGSPPPDDSLNEHLQCAIDSILNLQQGPPGHGGSGRGDLGRVHGANLPNQHQTHPSYRQSMPPLSTQPSSAPMSQHSQVGGRGQNGNLVSQTHSR

Target ORF information:

RefSeq Version	XM_005159254.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio glioma tumor suppressor candidate region gene 1 (gltscr1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159254.3

ORF Insert Sequence:

ATGGATGATG AAGATGGCAG GTGCTTGCTA GATGTAATTT GTGACCCACA AGCACTCAAC 
GATTTTCTTC ATGGATCAGA AACACAACTG GACACTGATG ACCTATTGGA TGGCTCAGGT 
GACCCGTCCA GCTCGTTCTT CTCCAGCACT GGGGGCCATG TTCCTGAGGT TCCGGCACAG 
GTTCAGCTGT CGACAAGTGA GCCGGGCCTT CCAAGAGTCA GCGTTGATTT GGACTTCTTG 
GAGGACGATG ACATATTGGG GGGATCGCCA GGAGGAGACA ATGGAAGTAA TGGTGTTGGG 
ACAAATCATG AGCCCTGTGA CATTTTGCAG CAGAGCTTGG CGGAGGCTAA CATCACAGAG 
CAGAGCCTTC AAGAGGCAGA TGCTGAGCTT GACCTCAGTT CCTTTGGGCT AACTGGCCTT 
ACGCAGGTGG TTCAACCCCT GCCTGATGCC GGCCTGTCTG GGGTGGGCAT TGGAGGTGCA 
ACTCAGATTT TTCCAAACCA AGGCACCCCT ACCGCACCCT CAAATCCCAC TCCAGACATG 
CTTGGTTCTG TCCTGGCACA TCCAGGTCTG CAGCTTCAAC AACAGGTCAT GAACAAGGCT 
ATCAGCGTCC AGCCTTTTGT ACAACAAGTC GGACTTGGAA ATGTAACACT TCAGCCAATT 
TCGAGCCTTC AGGCCCTACC GAACGGCAGC CAGTCTGGAC CGTTGGGCAT TGGACAAATT 
CAAGTGGTGG GTCAGCCCAC TGTAATGACT ATAAATCCAT CTGGGCAGCA AATCTTGACG 
AAAACCATGG GTGGCTACCA ACTGCATCAA CCTGGGCCGG AGGCAGCAAG CGCTGGAACG 
CAGGCTGGGC TTGGAAGCTC TGTTTTGAGT TCAGGAGGTG GACTTGTAAT TCAAGGGGGC 
AAGGCCACTC TCGGGTCCCC TGCCTTAAAT GGGCCAGCTG TCTGCTTAGG CAGCACAAAT 
ACCAACAACA GTGCAACTAC AATGGCCACT GCTGGTGGTA TAGTGGGTTT TAGTAATGCC 
TCTTTGGGTG CAGGAATTGG ATCTCAGACC CAGCCTCAAG GCCAAATCAT GCAGAATGTC 
ATCATCCAGC GAACGCCAAC GCCAATACAG CCCAAACCTC CTCAAGGGGG TACAATCCAA 
CCCAAAATCT TCAAGCAACA ACAACAACAG CTTCCTGCCC CACATGCCTT GCCAAATGAT 
GCTAATAAAG CTCTGGGGGT ACAGCAAATC CCAGTGTCAG CTGGTCAGAA TGTAACGTTC 
CTCACCGGGA AGCCTGGCTC TAACGTAGTT TTGAGCACAC AGGCAGCGTC GCAGGGAACA 
CAGTTTTCTC AAGCGCTCTT TAAACAGCAG GGCCCTCAAA CTTCAGGCAA ACCCCTCAGT 
GTTCACTTGT TAAACCAACA GGGCAGCATT GTCATTCCCT CGCAAACTGT CCTGCAGGGC 
CAAAACCACC AGTTCCTCTT ACCAGGGCTT CAAGCAGGAG GCCAGATCCT GGCTCAGCAT 
CCTGGTGGAC ACATCATAAC CAGTCAGGGG CCTGGAGGGC AGATAATCGC TAATCAGATC 
TTGACAGCCA ATCAGAACAT CAACTTGGGA CAGGTGTTGG CTTCACAGGG CCACCCTGGT 
GCAGCCCACA TTCTCTCTGG ACACATCCAG CTCCAGCCTG GTCAGATGGG TCAGCCTGCA 
CTGTTCCAAA TGCCGGTGTC TTTGGCGCAT ACTCAGACAC AATCTCATCC GGTAACCGGG 
CATGTACAAA CGGTCATTCA GGGCATGCCT ATACAGAATT CCCTGTCTGT GGAGAGCCTT 
AGCCCAGCAG TCAGTTTACA GACGTTGCAG CAGTCTGGCG GCGTCCCTAA TAATGGTAGC 
AGTGGATCGA CGGCAATGGC GCCATGCCAG CCAGGAGAGG GTATCACTGT ATTGGGCGGC 
TCTTCTGACT CTGCTGCTCA ACCAGCACAG ACGCAACCTC AGCCCTCAAT TCTCACGGTT 
CAGACGACGC CTCCAGTTTC AGTAGCAGCT TCGGTTCCTT CCTCTTCATC TCCATCCCCA 
ACGATGGCCA CGTCTGCATC CACAATGGTG GGTCTGTGTT CACAGGCCCA GCACAGCCCT 
GGAAAAGTGT TGTTCACGTC ACCTGGTTCC GGCATGATCC TGAGCCAGGA GCCTCTTCAA 
ATGTTCCTGC ACCAGGTTCC ATCGTCAGCA CATTCACAGT CTATAAAGAT CCAAAACGCC 
TCCCAATCTC AGCCTGTGGT CACCCCTTCG CCAGCACCCA CACTGTCCGA CAGCCCTCAA 
CCTGCTCAAG TTTCCCCTCT CACTCTCGGG CAGCAGATCC AGTCTCCCCA TCAACAGTCT 
CGGCCTCCCT CGCAGCCCCA GCCTCCTTCA CAGGCACAGA CTCCTTCACG CTCATGCACC 
CCTTCCTCTC TACCGCCTCT CTTCATCATT CACAATCAGA TGGGAGGTTC TCCACAGCCG 
GCTCCACCAC CTCAGCAACA GCAGCCTCAG CAGTTACAAG TGCAGCTCCA GCCTCAAGTT 
CTTCCTCAGC CTGCTGCTCT GCAGCCAGAC GTACCTCCTT CCTCGTGCTC CCCAAAGCCT 
CCGCAGCCAC TTCCCACACA GTTCCAGTTC CAGCCCCCAG TCTCCAGCTC GTCTCCAGCT 
GCAGCAGTGA AACAGCAGGT GACGGTGGTG CCTGGGCTGA CCGCAGAACA GCAGCATCAC 
CTACAGCTGG TCAGTGCGCA GCTGCAGACC ATGTCATCAA TCACACAGCC CTCCCCTCAA 
CAAAAGCAGC TCTTGGAAAA ACTTCACCAG GTCCAGCAAA ACATACTTTT GCAGGCTAAG 
CAGCAAGCTC AGGCACAAGC CCAGGCCTCT CAGCAACAGG CTACAAACCA GTTCAACAAA 
ATGCCAGATC AGCCTTCGGT CCAAGCTGCG ACTTCTTCAA CGGCCACTGG AGCCATCCAA 
GCACCAGTCC AGTCTCTTCT GCCGCAGAAG TCTGTGCTTG TCAAGCCCTC TACTACAGGT 
GCAAATGACA CACAAGTGTT TTCTGCGGTG TCTGCTGGGG CTACGGTGAA CACGGGAATC 
ACAACACCAA ACCTTGCACA GGCTGTCCAG GCAAAGCCAG GCGTCATAAG TTCGGTTAGT 
GGACTAACTC TGGGCAAAGC AGGTTTGCAG ATTCAGGTGT TAGGTGCTGG ACTCTCTCAA 
ATGCCTGCAC CTCCACTACC TGCTCCCCAA ACACAGACAT CAACATTAAA AAGGCCCTTT 
AGTATGGAAC CAAGCAAAGA AGCTAGGATG CTGGAACAGC TGCGAAAACA GCAAGGCTCA 
GTTCTCCATC CTGACTACAG CTCTCCTTTC CGCTCTTTTG AGGACACACT TCATCGGCTG 
CTGCCATACC ATCTGTACCA AGGCACGGCT TCATCTCCGG AAGACTGTCG TAAAGTGGAT 
GATGAATTTG AGAACGTCTC AAGCCAGCTC TTGAAACGCA CACAAGCAAT GCTGGATAAA 
TATCGTCACT TGCTTTTCGA GGAATCAAAG CAGAGGCTGG GTCCCTCAGC AGAGATGGTT 
ATGATTGACC GGATGTTCAT TCAAGAGGAA AAGGTTGCTC TGAGTCAGGA CAGGGTCCTA 
GCCAAGGAGA AACCAGAGGA ATTTGTGGCC AATTCGTGTT TAGTGGACAG CAGTGCGGTG 
AAGCTTGAAA AGGTGGAGCT AAGTTCTGTA AGAACTGCAT CAACAATGGT TGCGGCTCCG 
CCTACAGCAG TTACGCCTGC TCCTGCCACA ACTGTTGCCC CGGCTCCAAC CTCTACACCT 
GCTCCAGCTG CTGCCCCAAC TCCTGCTCCT GCTCCTGCCC CTGCACTCTT CCCTCCAACA 
AAACTGGTGA TCAAGCAAGG TGGAGGAGGA GCTTCAGTCT CCTGGTCCAC CAGCTCAACC 
CCTACGCCTG CTCCGGTGGT GCGACCGCCT GCTGAGCCTG TCGCACCAAG TGCCTCCTTC 
AGCCGCACTC CGTCCTCCCG CCCTGCTGAC GACGACGATG ACGTTGCACT TCCCCAACGG 
ACCAGCAAAC CACCCATCAA GACCTACGAG GCACGTCGGC GGATAGGTTT GAAGCTGAAG 
ATCAAGCAAG AGGCGGGGTT AAGTAAGGTG GTTCACAACA CTGCATTAGA TCCTGTCCAT 
TCGCAATCAC AACTCACACC ACAGACGCCC ACCCCCGAGC AGCCGCAGAA AGTAAAGCCA 
CACGTAACTC TTCCCTCCAC CGTCATTAGA ACTCAACCCA CAACCTCTCA ATCCACCTCC 
TCCTCCACTG TCACCACAGT AACCACACAC ACAGCCTCCG CCTCCAGTAG CGCAACTTCC 
TCGAGCGGGG CATCGTCTTC ATTCTCCACC TGGTCTTCAT CGTCACCTTC TACGTCGACC 
GCGCAGATGA ACGGCACCCT GGAGCACCAT GAGGTAGGTG GGGTCAAACG TAACCCAGCG 
TCCACAGCAA CGCCACCACT AACAACGTGC AGGCTCCCGC TCCGAAAGAC ATACCGTGAG 
AACATCAGTC CCCGTCACAG GCCTGGAGTA CCAGGAGGTG GTGGAGATAC ACTACCCATT 
CTGCCTGCAG GACCTCCTGT TACCTCTTCG CCCCAACCGC AAAGCTCGTC CCCTCAACCC 
GAACGAACTG TGATAGCCAG TGTGAAACTG GAGCGGCAAG GTGGACACGG GCGATCTCAC 
CCCCATGCGG AGTCACAAAG CCTGGCAGCC GTTGAAGACG TCCTTTATCG GGGAATAAAA 
AACGCGTACC AACATCATCG GGAGTTTGAT AAAGACGATG AGGATGATAT GGAGGAAGGT 
GGTGGTGGTG GTGGTGCTTT GGGGCGGTTA AAGGGCATAG GAAGCAAACA CCGGGAAGGG 
GGACGGGGCG CCTTCAGGAT GGATCAGCAT GCCCCAGGGC CGCCCTCTCC CGGAGAGTCT 
TCCTGCACAA GAGACTCGTC ACTTCCTGCC AAACGCTGCA AGTCGGACTC TCCGGACATG 
GACAATGCAA GCTTCTCCAG CGGCAGCCCG CCGCCTGACG ACTCGCTAAA TGAGCACCTG 
CAGTGTGCCA TAGACAGTAT ACTAAACCTC CAACAGGGCC CTCCTGGACA CGGAGGCTCA 
GGGAGAGGGG ATTTAGGGAG GGTTCATGGG GCAAACCTGC CTAACCAGCA CCAAACCCAT 
CCCTCCTACA GACAGTCCAT GCCTCCCCTC TCAACACAGC CCTCGTCCGC CCCCATGTCC 
CAGCATTCAC AGGTAGGAGG GCGAGGGCAG AATGGAAATC TGGTTTCACA GACGCACAGT 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bicra ( XM_005159254.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159311.1 Danio rerio BRD4 interacting chromatin remodeling complex associated protein (bicra), transcript variant X4, mRNA.

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CloneID	ODa23561
Clone ID Related Accession (Same CDS sequence)	XM_005159254.3 , XM_005159254.4
Accession Version	XM_005159254.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	glioma tumor suppressor candidate region gene 1 protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159254.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATG AAGATGGCAG GTGCTTGCTA GATGTAATTT GTGACCCACA AGCACTCAAC 
GATTTTCTTC ATGGATCAGA AACACAACTG GACACTGATG ACCTATTGGA TGGCTCAGGT 
GACCCGTCCA GCTCGTTCTT CTCCAGCACT GGGGGCCATG TTCCTGAGGT TCCGGCACAG 
GTTCAGCTGT CGACAAGTGA GCCGGGCCTT CCAAGAGTCA GCGTTGATTT GGACTTCTTG 
GAGGACGATG ACATATTGGG GGGATCGCCA GGAGGAGACA ATGGAAGTAA TGGTGTTGGG 
ACAAATCATG AGCCCTGTGA CATTTTGCAG CAGAGCTTGG CGGAGGCTAA CATCACAGAG 
CAGAGCCTTC AAGAGGCAGA TGCTGAGCTT GACCTCAGTT CCTTTGGGCT AACTGGCCTT 
ACGCAGGTGG TTCAACCCCT GCCTGATGCC GGCCTGTCTG GGGTGGGCAT TGGAGGTGCA 
ACTCAGATTT TTCCAAACCA AGGCACCCCT ACCGCACCCT CAAATCCCAC TCCAGACATG 
CTTGGTTCTG TCCTGGCACA TCCAGGTCTG CAGCTTCAAC AACAGGTCAT GAACAAGGCT 
ATCAGCGTCC AGCCTTTTGT ACAACAAGTC GGACTTGGAA ATGTAACACT TCAGCCAATT 
TCGAGCCTTC AGGCCCTACC GAACGGCAGC CAGTCTGGAC CGTTGGGCAT TGGACAAATT 
CAAGTGGTGG GTCAGCCCAC TGTAATGACT ATAAATCCAT CTGGGCAGCA AATCTTGACG 
AAAACCATGG GTGGCTACCA ACTGCATCAA CCTGGGCCGG AGGCAGCAAG CGCTGGAACG 
CAGGCTGGGC TTGGAAGCTC TGTTTTGAGT TCAGGAGGTG GACTTGTAAT TCAAGGGGGC 
AAGGCCACTC TCGGGTCCCC TGCCTTAAAT GGGCCAGCTG TCTGCTTAGG CAGCACAAAT 
ACCAACAACA GTGCAACTAC AATGGCCACT GCTGGTGGTA TAGTGGGTTT TAGTAATGCC 
TCTTTGGGTG CAGGAATTGG ATCTCAGACC CAGCCTCAAG GCCAAATCAT GCAGAATGTC 
ATCATCCAGC GAACGCCAAC GCCAATACAG CCCAAACCTC CTCAAGGGGG TACAATCCAA 
CCCAAAATCT TCAAGCAACA ACAACAACAG CTTCCTGCCC CACATGCCTT GCCAAATGAT 
GCTAATAAAG CTCTGGGGGT ACAGCAAATC CCAGTGTCAG CTGGTCAGAA TGTAACGTTC 
CTCACCGGGA AGCCTGGCTC TAACGTAGTT TTGAGCACAC AGGCAGCGTC GCAGGGAACA 
CAGTTTTCTC AAGCGCTCTT TAAACAGCAG GGCCCTCAAA CTTCAGGCAA ACCCCTCAGT 
GTTCACTTGT TAAACCAACA GGGCAGCATT GTCATTCCCT CGCAAACTGT CCTGCAGGGC 
CAAAACCACC AGTTCCTCTT ACCAGGGCTT CAAGCAGGAG GCCAGATCCT GGCTCAGCAT 
CCTGGTGGAC ACATCATAAC CAGTCAGGGG CCTGGAGGGC AGATAATCGC TAATCAGATC 
TTGACAGCCA ATCAGAACAT CAACTTGGGA CAGGTGTTGG CTTCACAGGG CCACCCTGGT 
GCAGCCCACA TTCTCTCTGG ACACATCCAG CTCCAGCCTG GTCAGATGGG TCAGCCTGCA 
CTGTTCCAAA TGCCGGTGTC TTTGGCGCAT ACTCAGACAC AATCTCATCC GGTAACCGGG 
CATGTACAAA CGGTCATTCA GGGCATGCCT ATACAGAATT CCCTGTCTGT GGAGAGCCTT 
AGCCCAGCAG TCAGTTTACA GACGTTGCAG CAGTCTGGCG GCGTCCCTAA TAATGGTAGC 
AGTGGATCGA CGGCAATGGC GCCATGCCAG CCAGGAGAGG GTATCACTGT ATTGGGCGGC 
TCTTCTGACT CTGCTGCTCA ACCAGCACAG ACGCAACCTC AGCCCTCAAT TCTCACGGTT 
CAGACGACGC CTCCAGTTTC AGTAGCAGCT TCGGTTCCTT CCTCTTCATC TCCATCCCCA 
ACGATGGCCA CGTCTGCATC CACAATGGTG GGTCTGTGTT CACAGGCCCA GCACAGCCCT 
GGAAAAGTGT TGTTCACGTC ACCTGGTTCC GGCATGATCC TGAGCCAGGA GCCTCTTCAA 
ATGTTCCTGC ACCAGGTTCC ATCGTCAGCA CATTCACAGT CTATAAAGAT CCAAAACGCC 
TCCCAATCTC AGCCTGTGGT CACCCCTTCG CCAGCACCCA CACTGTCCGA CAGCCCTCAA 
CCTGCTCAAG TTTCCCCTCT CACTCTCGGG CAGCAGATCC AGTCTCCCCA TCAACAGTCT 
CGGCCTCCCT CGCAGCCCCA GCCTCCTTCA CAGGCACAGA CTCCTTCACG CTCATGCACC 
CCTTCCTCTC TACCGCCTCT CTTCATCATT CACAATCAGA TGGGAGGTTC TCCACAGCCG 
GCTCCACCAC CTCAGCAACA GCAGCCTCAG CAGTTACAAG TGCAGCTCCA GCCTCAAGTT 
CTTCCTCAGC CTGCTGCTCT GCAGCCAGAC GTACCTCCTT CCTCGTGCTC CCCAAAGCCT 
CCGCAGCCAC TTCCCACACA GTTCCAGTTC CAGCCCCCAG TCTCCAGCTC GTCTCCAGCT 
GCAGCAGTGA AACAGCAGGT GACGGTGGTG CCTGGGCTGA CCGCAGAACA GCAGCATCAC 
CTACAGCTGG TCAGTGCGCA GCTGCAGACC ATGTCATCAA TCACACAGCC CTCCCCTCAA 
CAAAAGCAGC TCTTGGAAAA ACTTCACCAG GTCCAGCAAA ACATACTTTT GCAGGCTAAG 
CAGCAAGCTC AGGCACAAGC CCAGGCCTCT CAGCAACAGG CTACAAACCA GTTCAACAAA 
ATGCCAGATC AGCCTTCGGT CCAAGCTGCG ACTTCTTCAA CGGCCACTGG AGCCATCCAA 
GCACCAGTCC AGTCTCTTCT GCCGCAGAAG TCTGTGCTTG TCAAGCCCTC TACTACAGGT 
GCAAATGACA CACAAGTGTT TTCTGCGGTG TCTGCTGGGG CTACGGTGAA CACGGGAATC 
ACAACACCAA ACCTTGCACA GGCTGTCCAG GCAAAGCCAG GCGTCATAAG TTCGGTTAGT 
GGACTAACTC TGGGCAAAGC AGGTTTGCAG ATTCAGGTGT TAGGTGCTGG ACTCTCTCAA 
ATGCCTGCAC CTCCACTACC TGCTCCCCAA ACACAGACAT CAACATTAAA AAGGCCCTTT 
AGTATGGAAC CAAGCAAAGA AGCTAGGATG CTGGAACAGC TGCGAAAACA GCAAGGCTCA 
GTTCTCCATC CTGACTACAG CTCTCCTTTC CGCTCTTTTG AGGACACACT TCATCGGCTG 
CTGCCATACC ATCTGTACCA AGGCACGGCT TCATCTCCGG AAGACTGTCG TAAAGTGGAT 
GATGAATTTG AGAACGTCTC AAGCCAGCTC TTGAAACGCA CACAAGCAAT GCTGGATAAA 
TATCGTCACT TGCTTTTCGA GGAATCAAAG CAGAGGCTGG GTCCCTCAGC AGAGATGGTT 
ATGATTGACC GGATGTTCAT TCAAGAGGAA AAGGTTGCTC TGAGTCAGGA CAGGGTCCTA 
GCCAAGGAGA AACCAGAGGA ATTTGTGGCC AATTCGTGTT TAGTGGACAG CAGTGCGGTG 
AAGCTTGAAA AGGTGGAGCT AAGTTCTGTA AGAACTGCAT CAACAATGGT TGCGGCTCCG 
CCTACAGCAG TTACGCCTGC TCCTGCCACA ACTGTTGCCC CGGCTCCAAC CTCTACACCT 
GCTCCAGCTG CTGCCCCAAC TCCTGCTCCT GCTCCTGCCC CTGCACTCTT CCCTCCAACA 
AAACTGGTGA TCAAGCAAGG TGGAGGAGGA GCTTCAGTCT CCTGGTCCAC CAGCTCAACC 
CCTACGCCTG CTCCGGTGGT GCGACCGCCT GCTGAGCCTG TCGCACCAAG TGCCTCCTTC 
AGCCGCACTC CGTCCTCCCG CCCTGCTGAC GACGACGATG ACGTTGCACT TCCCCAACGG 
ACCAGCAAAC CACCCATCAA GACCTACGAG GCACGTCGGC GGATAGGTTT GAAGCTGAAG 
ATCAAGCAAG AGGCGGGGTT AAGTAAGGTG GTTCACAACA CTGCATTAGA TCCTGTCCAT 
TCGCAATCAC AACTCACACC ACAGACGCCC ACCCCCGAGC AGCCGCAGAA AGTAAAGCCA 
CACGTAACTC TTCCCTCCAC CGTCATTAGA ACTCAACCCA CAACCTCTCA ATCCACCTCC 
TCCTCCACTG TCACCACAGT AACCACACAC ACAGCCTCCG CCTCCAGTAG CGCAACTTCC 
TCGAGCGGGG CATCGTCTTC ATTCTCCACC TGGTCTTCAT CGTCACCTTC TACGTCGACC 
GCGCAGATGA ACGGCACCCT GGAGCACCAT GAGGTAGGTG GGGTCAAACG TAACCCAGCG 
TCCACAGCAA CGCCACCACT AACAACGTGC AGGCTCCCGC TCCGAAAGAC ATACCGTGAG 
AACATCAGTC CCCGTCACAG GCCTGGAGTA CCAGGAGGTG GTGGAGATAC ACTACCCATT 
CTGCCTGCAG GACCTCCTGT TACCTCTTCG CCCCAACCGC AAAGCTCGTC CCCTCAACCC 
GAACGAACTG TGATAGCCAG TGTGAAACTG GAGCGGCAAG GTGGACACGG GCGATCTCAC 
CCCCATGCGG AGTCACAAAG CCTGGCAGCC GTTGAAGACG TCCTTTATCG GGGAATAAAA 
AACGCGTACC AACATCATCG GGAGTTTGAT AAAGACGATG AGGATGATAT GGAGGAAGGT 
GGTGGTGGTG GTGGTGCTTT GGGGCGGTTA AAGGGCATAG GAAGCAAACA CCGGGAAGGG 
GGACGGGGCG CCTTCAGGAT GGATCAGCAT GCCCCAGGGC CGCCCTCTCC CGGAGAGTCT 
TCCTGCACAA GAGACTCGTC ACTTCCTGCC AAACGCTGCA AGTCGGACTC TCCGGACATG 
GACAATGCAA GCTTCTCCAG CGGCAGCCCG CCGCCTGACG ACTCGCTAAA TGAGCACCTG 
CAGTGTGCCA TAGACAGTAT ACTAAACCTC CAACAGGGCC CTCCTGGACA CGGAGGCTCA 
GGGAGAGGGG ATTTAGGGAG GGTTCATGGG GCAAACCTGC CTAACCAGCA CCAAACCCAT 
CCCTCCTACA GACAGTCCAT GCCTCCCCTC TCAACACAGC CCTCGTCCGC CCCCATGTCC 
CAGCATTCAC AGGTAGGAGG GCGAGGGCAG AATGGAAATC TGGTTTCACA GACGCACAGT 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159311.1
CDS	950..6235
Translation	MDDEDGRCLLDVICDPQALNDFLHGSETQLDTDDLLDGSGDPSSSFFSSTGGHVPEVPAQVQLSTSEPGLPRVSVDLDFLEDDDILGGSPGGDNGSNGVGTNHEPCDILQQSLAEANITEQSLQEADAELDLSSFGLTGLTQVVQPLPDAGLSGVGIGGATQIFPNQGTPTAPSNPTPDMLGSVLAHPGLQLQQQVMNKAISVQPFVQQVGLGNVTLQPISSLQALPNGSQSGPLGIGQIQVVGQPTVMTINPSGQQILTKTMGGYQLHQPGPEAASAGTQAGLGSSVLSSGGGLVIQGGKATLGSPALNGPAVCLGSTNTNNSATTMATAGGIVGFSNASLGAGIGSQTQPQGQIMQNVIIQRTPTPIQPKPPQGGTIQPKIFKQQQQQLPAPHALPNDANKALGVQQIPVSAGQNVTFLTGKPGSNVVLSTQAASQGTQFSQALFKQQGPQTSGKPLSVHLLNQQGSIVIPSQTVLQGQNHQFLLPGLQAGGQILAQHPGGHIITSQGPGGQIIANQILTANQNINLGQVLASQGHPGAAHILSGHIQLQPGQMGQPALFQMPVSLAHTQTQSHPVTGHVQTVIQGMPIQNSLSVESLSPAVSLQTLQQSGGVPNNGSSGSTAMAPCQPGEGITVLGGSSDSAAQPAQTQPQPSILTVQTTPPVSVAASVPSSSSPSPTMATSASTMVGLCSQAQHSPGKVLFTSPGSGMILSQEPLQMFLHQVPSSAHSQSIKIQNASQSQPVVTPSPAPTLSDSPQPAQVSPLTLGQQIQSPHQQSRPPSQPQPPSQAQTPSRSCTPSSLPPLFIIHNQMGGSPQPAPPPQQQQPQQLQVQLQPQVLPQPAALQPDVPPSSCSPKPPQPLPTQFQFQPPVSSSSPAAAVKQQVTVVPGLTAEQQHHLQLVSAQLQTMSSITQPSPQQKQLLEKLHQVQQNILLQAKQQAQAQAQASQQQATNQFNKMPDQPSVQAATSSTATGAIQAPVQSLLPQKSVLVKPSTTGANDTQVFSAVSAGATVNTGITTPNLAQAVQAKPGVISSVSGLTLGKAGLQIQVLGAGLSQMPAPPLPAPQTQTSTLKRPFSMEPSKEARMLEQLRKQQGSVLHPDYSSPFRSFEDTLHRLLPYHLYQGTASSPEDCRKVDDEFENVSSQLLKRTQAMLDKYRHLLFEESKQRLGPSAEMVMIDRMFIQEEKVALSQDRVLAKEKPEEFVANSCLVDSSAVKLEKVELSSVRTASTMVAAPPTAVTPAPATTVAPAPTSTPAPAAAPTPAPAPAPALFPPTKLVIKQGGGGASVSWSTSSTPTPAPVVRPPAEPVAPSASFSRTPSSRPADDDDDVALPQRTSKPPIKTYEARRRIGLKLKIKQEAGLSKVVHNTALDPVHSQSQLTPQTPTPEQPQKVKPHVTLPSTVIRTQPTTSQSTSSSTVTTVTTHTASASSSATSSSGASSSFSTWSSSSPSTSTAQMNGTLEHHEVGGVKRNPASTATPPLTTCRLPLRKTYRENISPRHRPGVPGGGGDTLPILPAGPPVTSSPQPQSSSPQPERTVIASVKLERQGGHGRSHPHAESQSLAAVEDVLYRGIKNAYQHHREFDKDDEDDMEEGGGGGGALGRLKGIGSKHREGGRGAFRMDQHAPGPPSPGESSCTRDSSLPAKRCKSDSPDMDNASFSSGSPPPDDSLNEHLQCAIDSILNLQQGPPGHGGSGRGDLGRVHGANLPNQHQTHPSYRQSMPPLSTQPSSAPMSQHSQVGGRGQNGNLVSQTHSR

Target ORF information:

RefSeq Version	XM_005159254.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio BRD4 interacting chromatin remodeling complex associated protein (bicra), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159254.4

ORF Insert Sequence:

ATGGATGATG AAGATGGCAG GTGCTTGCTA GATGTAATTT GTGACCCACA AGCACTCAAC 
GATTTTCTTC ATGGATCAGA AACACAACTG GACACTGATG ACCTATTGGA TGGCTCAGGT 
GACCCGTCCA GCTCGTTCTT CTCCAGCACT GGGGGCCATG TTCCTGAGGT TCCGGCACAG 
GTTCAGCTGT CGACAAGTGA GCCGGGCCTT CCAAGAGTCA GCGTTGATTT GGACTTCTTG 
GAGGACGATG ACATATTGGG GGGATCGCCA GGAGGAGACA ATGGAAGTAA TGGTGTTGGG 
ACAAATCATG AGCCCTGTGA CATTTTGCAG CAGAGCTTGG CGGAGGCTAA CATCACAGAG 
CAGAGCCTTC AAGAGGCAGA TGCTGAGCTT GACCTCAGTT CCTTTGGGCT AACTGGCCTT 
ACGCAGGTGG TTCAACCCCT GCCTGATGCC GGCCTGTCTG GGGTGGGCAT TGGAGGTGCA 
ACTCAGATTT TTCCAAACCA AGGCACCCCT ACCGCACCCT CAAATCCCAC TCCAGACATG 
CTTGGTTCTG TCCTGGCACA TCCAGGTCTG CAGCTTCAAC AACAGGTCAT GAACAAGGCT 
ATCAGCGTCC AGCCTTTTGT ACAACAAGTC GGACTTGGAA ATGTAACACT TCAGCCAATT 
TCGAGCCTTC AGGCCCTACC GAACGGCAGC CAGTCTGGAC CGTTGGGCAT TGGACAAATT 
CAAGTGGTGG GTCAGCCCAC TGTAATGACT ATAAATCCAT CTGGGCAGCA AATCTTGACG 
AAAACCATGG GTGGCTACCA ACTGCATCAA CCTGGGCCGG AGGCAGCAAG CGCTGGAACG 
CAGGCTGGGC TTGGAAGCTC TGTTTTGAGT TCAGGAGGTG GACTTGTAAT TCAAGGGGGC 
AAGGCCACTC TCGGGTCCCC TGCCTTAAAT GGGCCAGCTG TCTGCTTAGG CAGCACAAAT 
ACCAACAACA GTGCAACTAC AATGGCCACT GCTGGTGGTA TAGTGGGTTT TAGTAATGCC 
TCTTTGGGTG CAGGAATTGG ATCTCAGACC CAGCCTCAAG GCCAAATCAT GCAGAATGTC 
ATCATCCAGC GAACGCCAAC GCCAATACAG CCCAAACCTC CTCAAGGGGG TACAATCCAA 
CCCAAAATCT TCAAGCAACA ACAACAACAG CTTCCTGCCC CACATGCCTT GCCAAATGAT 
GCTAATAAAG CTCTGGGGGT ACAGCAAATC CCAGTGTCAG CTGGTCAGAA TGTAACGTTC 
CTCACCGGGA AGCCTGGCTC TAACGTAGTT TTGAGCACAC AGGCAGCGTC GCAGGGAACA 
CAGTTTTCTC AAGCGCTCTT TAAACAGCAG GGCCCTCAAA CTTCAGGCAA ACCCCTCAGT 
GTTCACTTGT TAAACCAACA GGGCAGCATT GTCATTCCCT CGCAAACTGT CCTGCAGGGC 
CAAAACCACC AGTTCCTCTT ACCAGGGCTT CAAGCAGGAG GCCAGATCCT GGCTCAGCAT 
CCTGGTGGAC ACATCATAAC CAGTCAGGGG CCTGGAGGGC AGATAATCGC TAATCAGATC 
TTGACAGCCA ATCAGAACAT CAACTTGGGA CAGGTGTTGG CTTCACAGGG CCACCCTGGT 
GCAGCCCACA TTCTCTCTGG ACACATCCAG CTCCAGCCTG GTCAGATGGG TCAGCCTGCA 
CTGTTCCAAA TGCCGGTGTC TTTGGCGCAT ACTCAGACAC AATCTCATCC GGTAACCGGG 
CATGTACAAA CGGTCATTCA GGGCATGCCT ATACAGAATT CCCTGTCTGT GGAGAGCCTT 
AGCCCAGCAG TCAGTTTACA GACGTTGCAG CAGTCTGGCG GCGTCCCTAA TAATGGTAGC 
AGTGGATCGA CGGCAATGGC GCCATGCCAG CCAGGAGAGG GTATCACTGT ATTGGGCGGC 
TCTTCTGACT CTGCTGCTCA ACCAGCACAG ACGCAACCTC AGCCCTCAAT TCTCACGGTT 
CAGACGACGC CTCCAGTTTC AGTAGCAGCT TCGGTTCCTT CCTCTTCATC TCCATCCCCA 
ACGATGGCCA CGTCTGCATC CACAATGGTG GGTCTGTGTT CACAGGCCCA GCACAGCCCT 
GGAAAAGTGT TGTTCACGTC ACCTGGTTCC GGCATGATCC TGAGCCAGGA GCCTCTTCAA 
ATGTTCCTGC ACCAGGTTCC ATCGTCAGCA CATTCACAGT CTATAAAGAT CCAAAACGCC 
TCCCAATCTC AGCCTGTGGT CACCCCTTCG CCAGCACCCA CACTGTCCGA CAGCCCTCAA 
CCTGCTCAAG TTTCCCCTCT CACTCTCGGG CAGCAGATCC AGTCTCCCCA TCAACAGTCT 
CGGCCTCCCT CGCAGCCCCA GCCTCCTTCA CAGGCACAGA CTCCTTCACG CTCATGCACC 
CCTTCCTCTC TACCGCCTCT CTTCATCATT CACAATCAGA TGGGAGGTTC TCCACAGCCG 
GCTCCACCAC CTCAGCAACA GCAGCCTCAG CAGTTACAAG TGCAGCTCCA GCCTCAAGTT 
CTTCCTCAGC CTGCTGCTCT GCAGCCAGAC GTACCTCCTT CCTCGTGCTC CCCAAAGCCT 
CCGCAGCCAC TTCCCACACA GTTCCAGTTC CAGCCCCCAG TCTCCAGCTC GTCTCCAGCT 
GCAGCAGTGA AACAGCAGGT GACGGTGGTG CCTGGGCTGA CCGCAGAACA GCAGCATCAC 
CTACAGCTGG TCAGTGCGCA GCTGCAGACC ATGTCATCAA TCACACAGCC CTCCCCTCAA 
CAAAAGCAGC TCTTGGAAAA ACTTCACCAG GTCCAGCAAA ACATACTTTT GCAGGCTAAG 
CAGCAAGCTC AGGCACAAGC CCAGGCCTCT CAGCAACAGG CTACAAACCA GTTCAACAAA 
ATGCCAGATC AGCCTTCGGT CCAAGCTGCG ACTTCTTCAA CGGCCACTGG AGCCATCCAA 
GCACCAGTCC AGTCTCTTCT GCCGCAGAAG TCTGTGCTTG TCAAGCCCTC TACTACAGGT 
GCAAATGACA CACAAGTGTT TTCTGCGGTG TCTGCTGGGG CTACGGTGAA CACGGGAATC 
ACAACACCAA ACCTTGCACA GGCTGTCCAG GCAAAGCCAG GCGTCATAAG TTCGGTTAGT 
GGACTAACTC TGGGCAAAGC AGGTTTGCAG ATTCAGGTGT TAGGTGCTGG ACTCTCTCAA 
ATGCCTGCAC CTCCACTACC TGCTCCCCAA ACACAGACAT CAACATTAAA AAGGCCCTTT 
AGTATGGAAC CAAGCAAAGA AGCTAGGATG CTGGAACAGC TGCGAAAACA GCAAGGCTCA 
GTTCTCCATC CTGACTACAG CTCTCCTTTC CGCTCTTTTG AGGACACACT TCATCGGCTG 
CTGCCATACC ATCTGTACCA AGGCACGGCT TCATCTCCGG AAGACTGTCG TAAAGTGGAT 
GATGAATTTG AGAACGTCTC AAGCCAGCTC TTGAAACGCA CACAAGCAAT GCTGGATAAA 
TATCGTCACT TGCTTTTCGA GGAATCAAAG CAGAGGCTGG GTCCCTCAGC AGAGATGGTT 
ATGATTGACC GGATGTTCAT TCAAGAGGAA AAGGTTGCTC TGAGTCAGGA CAGGGTCCTA 
GCCAAGGAGA AACCAGAGGA ATTTGTGGCC AATTCGTGTT TAGTGGACAG CAGTGCGGTG 
AAGCTTGAAA AGGTGGAGCT AAGTTCTGTA AGAACTGCAT CAACAATGGT TGCGGCTCCG 
CCTACAGCAG TTACGCCTGC TCCTGCCACA ACTGTTGCCC CGGCTCCAAC CTCTACACCT 
GCTCCAGCTG CTGCCCCAAC TCCTGCTCCT GCTCCTGCCC CTGCACTCTT CCCTCCAACA 
AAACTGGTGA TCAAGCAAGG TGGAGGAGGA GCTTCAGTCT CCTGGTCCAC CAGCTCAACC 
CCTACGCCTG CTCCGGTGGT GCGACCGCCT GCTGAGCCTG TCGCACCAAG TGCCTCCTTC 
AGCCGCACTC CGTCCTCCCG CCCTGCTGAC GACGACGATG ACGTTGCACT TCCCCAACGG 
ACCAGCAAAC CACCCATCAA GACCTACGAG GCACGTCGGC GGATAGGTTT GAAGCTGAAG 
ATCAAGCAAG AGGCGGGGTT AAGTAAGGTG GTTCACAACA CTGCATTAGA TCCTGTCCAT 
TCGCAATCAC AACTCACACC ACAGACGCCC ACCCCCGAGC AGCCGCAGAA AGTAAAGCCA 
CACGTAACTC TTCCCTCCAC CGTCATTAGA ACTCAACCCA CAACCTCTCA ATCCACCTCC 
TCCTCCACTG TCACCACAGT AACCACACAC ACAGCCTCCG CCTCCAGTAG CGCAACTTCC 
TCGAGCGGGG CATCGTCTTC ATTCTCCACC TGGTCTTCAT CGTCACCTTC TACGTCGACC 
GCGCAGATGA ACGGCACCCT GGAGCACCAT GAGGTAGGTG GGGTCAAACG TAACCCAGCG 
TCCACAGCAA CGCCACCACT AACAACGTGC AGGCTCCCGC TCCGAAAGAC ATACCGTGAG 
AACATCAGTC CCCGTCACAG GCCTGGAGTA CCAGGAGGTG GTGGAGATAC ACTACCCATT 
CTGCCTGCAG GACCTCCTGT TACCTCTTCG CCCCAACCGC AAAGCTCGTC CCCTCAACCC 
GAACGAACTG TGATAGCCAG TGTGAAACTG GAGCGGCAAG GTGGACACGG GCGATCTCAC 
CCCCATGCGG AGTCACAAAG CCTGGCAGCC GTTGAAGACG TCCTTTATCG GGGAATAAAA 
AACGCGTACC AACATCATCG GGAGTTTGAT AAAGACGATG AGGATGATAT GGAGGAAGGT 
GGTGGTGGTG GTGGTGCTTT GGGGCGGTTA AAGGGCATAG GAAGCAAACA CCGGGAAGGG 
GGACGGGGCG CCTTCAGGAT GGATCAGCAT GCCCCAGGGC CGCCCTCTCC CGGAGAGTCT 
TCCTGCACAA GAGACTCGTC ACTTCCTGCC AAACGCTGCA AGTCGGACTC TCCGGACATG 
GACAATGCAA GCTTCTCCAG CGGCAGCCCG CCGCCTGACG ACTCGCTAAA TGAGCACCTG 
CAGTGTGCCA TAGACAGTAT ACTAAACCTC CAACAGGGCC CTCCTGGACA CGGAGGCTCA 
GGGAGAGGGG ATTTAGGGAG GGTTCATGGG GCAAACCTGC CTAACCAGCA CCAAACCCAT 
CCCTCCTACA GACAGTCCAT GCCTCCCCTC TCAACACAGC CCTCGTCCGC CCCCATGTCC 
CAGCATTCAC AGGTAGGAGG GCGAGGGCAG AATGGAAATC TGGTTTCACA GACGCACAGT 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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bicra cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

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