gtf2h4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gtf2h4
Entrez Gene ID	334839
Full Name	general transcription factor IIH, polypeptide 4
Synonyms	wu:fa04h09,zgc:77721
General protein information	Preferred Names general transcription factor IIH, polypeptide 4 Names general transcription factor IIH subunit 4 p52
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199927.1	NP_956221.1	general transcription factor IIH subunit 4

Pathways from BioSystems

Homologies

	GTF2H4	NP_001040607.1
Danio rerio (zebrafish)	gtf2h4	NP_956221.1
Neurospora crassa	NCU05523	XP_960485.2
Arabidopsis thaliana (thale cress)	AT4G17020	NP_974564.1
Canis lupus familiaris (dog)	GTF2H4	XP_005627183.1
Drosophila melanogaster (fruit fly)	mrn	NP_648780.1
Schizosaccharomyces pombe (fission yeast)	tfb2	NP_596563.1
Kluyveromyces lactis	KLLA0F01056g	XP_455127.1
Eremothecium gossypii	AGOS_ADL274W	NP_983822.1
Xenopus tropicalis (tropical clawed frog)	gtf2h4	NP_001015845.1
Bos taurus (cattle)	GTF2H4	NP_001094527.1
Mus musculus (house mouse)	Gtf2h4	NP_034494.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010984	XP_309710.1
Saccharomyces cerevisiae (baker's yeast)	TFB2	NP_015203.1
Magnaporthe oryzae (rice blast fungus)	MGG_00397	XP_003718679.1
Homo sapiens (human)	GTF2H4	NP_001508.1
Pan troglodytes (chimpanzee)	GTF2H4	NP_001038968.1
Rattus norvegicus (Norway rat)	Gtf2h4	NP_997666.1
Oryza sativa (rice)	Os04g0680000	NP_001054284.1
Xenopus tropicalis (tropical clawed frog)	LOC101731871	XP_004911969.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Evolutionary conservation of TFIIH subunits: implications for the use of zebrafish as a model to study TFIIH function and regulation.
Silva IA, Cox CJ, Leite RB, Cancela ML, Concei??o N
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology172-1739-20(2014 Jun-Jul)

Zebrafish mRNA sequencing deciphers novelties in transcriptome dynamics during maternal to zygotic transition.
Aanes H, Winata CL, Lin CH, Chen JP, Srinivasan KG, Lee SG, Lim AY, Hajan HS, Collas P, Bourque G, Gong Z, Korzh V, Alestr?m P, Mathavan S
Genome research21(8)1328-38(2011 Aug)

A genome-wide survey of Major Histocompatibility Complex (MHC) genes and their paralogues in zebrafish.
Sambrook JG, Figueroa F, Beck S
BMC genomics6152(2005 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gtf2h4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gtf2h4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36939	NM_199927.1 Latest version!	Danio rerio general transcription factor IIH, polypeptide 4 (gtf2h4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gtf2h4 ( NM_199927.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956221.1 Danio rerio general transcription factor IIH, polypeptide 4 (gtf2h4), mRNA.

Price & Availability↑

CloneID	ODa36939
Clone ID Related Accession (Same CDS sequence)	NM_199927.1
Accession Version	NM_199927.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	general transcription factor IIH subunit 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC064301.1. ##Evidence-Data-START## Transcript exon combination :: BC064301.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAAGCTTC GCGTTCAGCT TCAGTGTAAG AACTTGCACG AGTATCTGAA AGAACTGACC 
CCAGAAATCC TCGACAGGCT CTACAATCAC CCAGCAACAT GTCTAGCAGT ATACAGGGAA 
CTTCCACCAC TGGCAAAGAA TTACGTGATG CGCATGCTGT TTTTAGACCA TCCACTCCCC 
CAGGCTGCGG TTGCCCTGTG GGTTAAAAAA GGCAGTCAAA AAGACCATGA TCAATGTGTG 
TCGGTGTTAA CTGGGCTACG GCTCTGGCAC AGTCAGCATC TTCAAGGTGG ACTTCAAGGT 
TTCGTGCTCA ACCCCGTGTT TAAGGACAAC CTGAGAATAG CACTTCTAGG CGGTGGTAAA 
CCGTGGGCAG ACGAGGGTGC CAATCTGGGT CCCGATCGGC ATGCGAGGGA TGTAGAGAGT 
CTGGACCGCT ATGCTATGGA GCGCTGGGAG GTCATCCTTC ACTTCATGGT GGGCTCTCCT 
AGCGCAGCTG TCAGTCAGGA TCTGGCTCAG CTCCTCATAC AGGCCGGGCT CATGAAGAGC 
GAGACTGGTG AGGCTCCATG CATCACATCA GCAGGGTTTC AGTTCCTGCT GCTGGACACG 
GCATCTCAGT TGTGGTACTT CACCCTGCAG TACCTCAAAA CAGCACAGTC GAGAGGAATG 
GACCTGGTGG AGATTCTTTC CTTTTTGTTC CAGCTGAGTT TTTCCACTCT TGGAAGGGAT 
TATTCTGTGG AGGGCATGAG CGAGTCCCTG CTCACATTTT TGCAGCATCT ACGAGAGTTT 
GGTCTGGTTT TTCAAAGAAA GAGGAAGTCA AGAAGGTACT ACCCTACGAG ACTCGCCATA 
ACTCTCGCTG CAGGAGTCAC TGCAAACCCA GCCTCGGGAT CTGCCTCTTC TGCACTGGGA 
GCAATACCAG GAACAGGAGA CACTGGATTC ATCGTTGTAG AAACTAATTA TCGCATATAT 
GCCTATACAA ACTCTGAACT TCAGATTGCA CTGGTCGCTC TGTTTAGTGA GATGTTGTAT 
CGGTTTCCTA ACCTGGTGGT TGCACAGGTC ACCCGAGAGT CTGTGCAGCA GGCCATCAGT 
AACGGCATCA CCGCACAGCA GATTATTCAC TTCTTACGGA CCCGAGCACA CCCAGTTATG 
CTTAAACAGA CCCCCGTTCT CCCCCCAACC ATCACAGATC AGATCAGACT GTGGGAGCTT 
GAGAAAGATC GCTTGCAGTT TACCGAGGGA GTGTTATACA ACCAGTTTTT ATCCCAGGCT 
GATTTCGAAG TGTTGCGGGA CAGGGCTCAG GGTTTAGGAG TGCTGGTGTG GCAGAATCCC 
GCTCACAGGG TCATGGTGGT GACTCCTCAT GGACACAGCG AGGTTAAGAG ATTCTGGAAG 
AGACAAAAAA GCCATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956221.1
CDS	97..1497
Translation	MKLRVQLQCKNLHEYLKELTPEILDRLYNHPATCLAVYRELPPLAKNYVMRMLFLDHPLPQAAVALWVKKGSQKDHDQCVSVLTGLRLWHSQHLQGGLQGFVLNPVFKDNLRIALLGGGKPWADEGANLGPDRHARDVESLDRYAMERWEVILHFMVGSPSAAVSQDLAQLLIQAGLMKSETGEAPCITSAGFQFLLLDTASQLWYFTLQYLKTAQSRGMDLVEILSFLFQLSFSTLGRDYSVEGMSESLLTFLQHLREFGLVFQRKRKSRRYYPTRLAITLAAGVTANPASGSASSALGAIPGTGDTGFIVVETNYRIYAYTNSELQIALVALFSEMLYRFPNLVVAQVTRESVQQAISNGITAQQIIHFLRTRAHPVMLKQTPVLPPTITDQIRLWELEKDRLQFTEGVLYNQFLSQADFEVLRDRAQGLGVLVWQNPAHRVMVVTPHGHSEVKRFWKRQKSHS

Target ORF information:

RefSeq Version	NM_199927.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio general transcription factor IIH, polypeptide 4 (gtf2h4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199927.1

ORF Insert Sequence:

ATGAAGCTTC GCGTTCAGCT TCAGTGTAAG AACTTGCACG AGTATCTGAA AGAACTGACC 
CCAGAAATCC TCGACAGGCT CTACAATCAC CCAGCAACAT GTCTAGCAGT ATACAGGGAA 
CTTCCACCAC TGGCAAAGAA TTACGTGATG CGCATGCTGT TTTTAGACCA TCCACTCCCC 
CAGGCTGCGG TTGCCCTGTG GGTTAAAAAA GGCAGTCAAA AAGACCATGA TCAATGTGTG 
TCGGTGTTAA CTGGGCTACG GCTCTGGCAC AGTCAGCATC TTCAAGGTGG ACTTCAAGGT 
TTCGTGCTCA ACCCCGTGTT TAAGGACAAC CTGAGAATAG CACTTCTAGG CGGTGGTAAA 
CCGTGGGCAG ACGAGGGTGC CAATCTGGGT CCCGATCGGC ATGCGAGGGA TGTAGAGAGT 
CTGGACCGCT ATGCTATGGA GCGCTGGGAG GTCATCCTTC ACTTCATGGT GGGCTCTCCT 
AGCGCAGCTG TCAGTCAGGA TCTGGCTCAG CTCCTCATAC AGGCCGGGCT CATGAAGAGC 
GAGACTGGTG AGGCTCCATG CATCACATCA GCAGGGTTTC AGTTCCTGCT GCTGGACACG 
GCATCTCAGT TGTGGTACTT CACCCTGCAG TACCTCAAAA CAGCACAGTC GAGAGGAATG 
GACCTGGTGG AGATTCTTTC CTTTTTGTTC CAGCTGAGTT TTTCCACTCT TGGAAGGGAT 
TATTCTGTGG AGGGCATGAG CGAGTCCCTG CTCACATTTT TGCAGCATCT ACGAGAGTTT 
GGTCTGGTTT TTCAAAGAAA GAGGAAGTCA AGAAGGTACT ACCCTACGAG ACTCGCCATA 
ACTCTCGCTG CAGGAGTCAC TGCAAACCCA GCCTCGGGAT CTGCCTCTTC TGCACTGGGA 
GCAATACCAG GAACAGGAGA CACTGGATTC ATCGTTGTAG AAACTAATTA TCGCATATAT 
GCCTATACAA ACTCTGAACT TCAGATTGCA CTGGTCGCTC TGTTTAGTGA GATGTTGTAT 
CGGTTTCCTA ACCTGGTGGT TGCACAGGTC ACCCGAGAGT CTGTGCAGCA GGCCATCAGT 
AACGGCATCA CCGCACAGCA GATTATTCAC TTCTTACGGA CCCGAGCACA CCCAGTTATG 
CTTAAACAGA CCCCCGTTCT CCCCCCAACC ATCACAGATC AGATCAGACT GTGGGAGCTT 
GAGAAAGATC GCTTGCAGTT TACCGAGGGA GTGTTATACA ACCAGTTTTT ATCCCAGGCT 
GATTTCGAAG TGTTGCGGGA CAGGGCTCAG GGTTTAGGAG TGCTGGTGTG GCAGAATCCC 
GCTCACAGGG TCATGGTGGT GACTCCTCAT GGACACAGCG AGGTTAAGAG ATTCTGGAAG 
AGACAAAAAA GCCATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Evolutionary conservation of TFIIH subunits: implications for the use of zebrafish as a model to study TFIIH function and regulation.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology172-1739-20(2014 Jun-Jul)
Silva IA,Cox CJ,Leite RB,Cancela ML,Concei??o N

Zebrafish mRNA sequencing deciphers novelties in transcriptome dynamics during maternal to zygotic transition.
Genome research21(8)1328-38(2011 Aug)
Aanes H,Winata CL,Lin CH,Chen JP,Srinivasan KG,Lee SG,Lim AY,Hajan HS,Collas P,Bourque G,Gong Z,Korzh V,Alestr?m P,Mathavan S

A genome-wide survey of Major Histocompatibility Complex (MHC) genes and their paralogues in zebrafish.
BMC genomics6152(2005 Nov)
Sambrook JG,Figueroa F,Beck S