bmi1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	bmi1a
Entrez Gene ID	321505
Full Name	bmi1 polycomb ring finger oncogene 1a
Synonyms	bmi1,pcgf4,psc1,wu:fb17g03,wu:fd18f06
General protein information	Preferred Names bmi1 polycomb ring finger oncogene 1a Names polycomb complex protein BMI-1-A B lymphoma Mo-MLV insertion region 1 homolog B lymphoma Mo-MLV insertion region 1a polycomb group RING finger protein 4-A polycomb group ring finger 4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_194366.1	NP_919347.1	polycomb complex protein BMI-1-A

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	bmi1a	NP_919347.1
Homo sapiens (human)	BMI1	NP_005171.4
Pan troglodytes (chimpanzee)	BMI1	XP_001147119.2

Gene Ontology
Bibliography

Related articles in PubMed

TOX Regulates Growth, DNA Repair, and Genomic Instability in T-cell Acute Lymphoblastic Leukemia.
Lobbardi R, Pinder J, Martinez-Pastor B, Theodorou M, Blackburn JS, Abraham BJ, Namiki Y, Mansour M, Abdelfattah NS, Molodtsov A, Alexe G, Toiber D, de Waard M, Jain E, Boukhali M, Lion M, Bhere D, Shah K, Gutierrez A, Stegmaier K, Silverman LB, Sadreyev RI, Asara JM, Oettinger MA, Haas W, Look AT, Young RA, Mostoslavsky R, Dellaire G, Langenau DM
Cancer discovery7(11)1336-1353(2017 11)

Identification and Expression Analysis of the Complete Family of Zebrafish
England SJ, Campbell PC, Banerjee S, Swanson AJ, Lewis KE
Frontiers in cell and developmental biology55(2017)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

The Polycomb Group Protein Pcgf1 Is Dispensable in Zebrafish but Involved in Early Growth and Aging.
Dupret B, V?lkel P, Le Bourhis X, Angrand PO
PloS one11(7)e0158700(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Bmi1 acts immediately downstream of CCAAT enhancer binding protein-alpha to regulate the survival and self-renewal of hematopoietic stem cells and contribute to the erythropoietic dysplasia.
Title: Dominant-negative C/ebp? and polycomb group protein Bmi1 extend short-lived hematopoietic stem/progenitor cell life span and induce lethal dyserythropoiesis.

10 genes were down-regulated following treatment of the T-ALL cells with 0.15 and 1.5 microg/mL of metal ores at 72 h. Cell growth maintenance, segment specification.
Title: Dose-dependent transcriptome changes by metal ores on a human acute lymphoblastic leukemia cell line.

The following bmi1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bmi1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02741	NM_194366.1 Latest version!	Danio rerio bmi1 polycomb ring finger oncogene 1a (bmi1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bmi1a ( NM_194366.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_919347.1 Danio rerio bmi1 polycomb ring finger oncogene 1a (bmi1a), mRNA.

Price & Availability↑

CloneID	ODa02741
Clone ID Related Accession (Same CDS sequence)	NM_194366.1
Accession Version	NM_194366.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-14
Organism	Danio rerio(zebrafish)
Product	polycomb complex protein BMI-1-A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB074153.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB074153.1, EH436779.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGACGATGC ATCGCACAAC AAGGATCAAG ATTACAGAGC TCAATCCTCA TCTGATGTGT 
GTATTATGTG GTGGATATTT CATAGATGCA ACAACAATCA TAGAATGCTT GCACTCATTT 
TGTAAAATGT GCATTGTCCG GTACCTGGAG ACCAGTAAAT ACTGCCCCAT ATGTGATGTG 
CAGGTTCACA AAACAAAGCC ACTTCTCAAT ATACGGTCTG ACAAAACTCT ACAAGACATT 
GTATACAAGT TGGTTCCTGG TCTTTTCAAA AATGAAATGA AGCGTAGGAG AGACTTTTAT 
GCTGAACACC CATCTGTTGA TGCTGCAAAT GGGTCAAATG AGGATCGAGG GGAAGTTGCT 
GACGAGGACA AGCGAATCAT TACAGATGAT GAGATTATCA GTCTGTCAAT TGAGTTTTTC 
GATCATAGGG CGCAACAGCA AGGCTGCACA GAGGAGAGAC AAAAAGAGGA GGTCAATAAC 
AAAAGATATC TACAATGCCC TGCTGCCATG ACTGTGATGC ATTTGAGGAA ATTTCTGAGA 
AGCAAAATGG ATATACCTCC TACTTACCAG ATCGAAGTTA TGTATGAGGA TGAGCCTTTG 
AAGGATTATT ACACATTAAT GGACATTGCC TACATCTACA CGTGGAGAAG AAATGGACCA 
CTGCCGCTAA AATACCGTGT GCGACCCAGC TGTAAAAAAA TGAAGATTAC CCACCCACAA 
GAGGGTTTGA ACAATGCAAA CCGATCTGAA AGCGACTCAG CCAGTGATAA GGCCTGCAGT 
CCTGCTGGAG TTCCATCCAC CTCCTCACCA CTACCGAGTC CGAGCACTCT GGTGCAGCCG 
TCACAACCTC ATTTCACTCA TATTTCCAGT CCTATTAACG GCACGACAAT GACAAGCCCA 
AATCGACAGT TCAACTTCAG CAAAGTGCGA AAGTCAGCAC TGAACGGATC TTCAACATCA 
TCTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_919347.1
CDS	341..1309
Translation	MTMHRTTRIKITELNPHLMCVLCGGYFIDATTIIECLHSFCKMCIVRYLETSKYCPICDVQVHKTKPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYAEHPSVDAANGSNEDRGEVADEDKRIITDDEIISLSIEFFDHRAQQQGCTEERQKEEVNNKRYLQCPAAMTVMHLRKFLRSKMDIPPTYQIEVMYEDEPLKDYYTLMDIAYIYTWRRNGPLPLKYRVRPSCKKMKITHPQEGLNNANRSESDSASDKACSPAGVPSTSSPLPSPSTLVQPSQPHFTHISSPINGTTMTSPNRQFNFSKVRKSALNGSSTSSG

Target ORF information:

RefSeq Version	NM_194366.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio bmi1 polycomb ring finger oncogene 1a (bmi1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_194366.1

ORF Insert Sequence:

ATGACGATGC ATCGCACAAC AAGGATCAAG ATTACAGAGC TCAATCCTCA TCTGATGTGT 
GTATTATGTG GTGGATATTT CATAGATGCA ACAACAATCA TAGAATGCTT GCACTCATTT 
TGTAAAATGT GCATTGTCCG GTACCTGGAG ACCAGTAAAT ACTGCCCCAT ATGTGATGTG 
CAGGTTCACA AAACAAAGCC ACTTCTCAAT ATACGGTCTG ACAAAACTCT ACAAGACATT 
GTATACAAGT TGGTTCCTGG TCTTTTCAAA AATGAAATGA AGCGTAGGAG AGACTTTTAT 
GCTGAACACC CATCTGTTGA TGCTGCAAAT GGGTCAAATG AGGATCGAGG GGAAGTTGCT 
GACGAGGACA AGCGAATCAT TACAGATGAT GAGATTATCA GTCTGTCAAT TGAGTTTTTC 
GATCATAGGG CGCAACAGCA AGGCTGCACA GAGGAGAGAC AAAAAGAGGA GGTCAATAAC 
AAAAGATATC TACAATGCCC TGCTGCCATG ACTGTGATGC ATTTGAGGAA ATTTCTGAGA 
AGCAAAATGG ATATACCTCC TACTTACCAG ATCGAAGTTA TGTATGAGGA TGAGCCTTTG 
AAGGATTATT ACACATTAAT GGACATTGCC TACATCTACA CGTGGAGAAG AAATGGACCA 
CTGCCGCTAA AATACCGTGT GCGACCCAGC TGTAAAAAAA TGAAGATTAC CCACCCACAA 
GAGGGTTTGA ACAATGCAAA CCGATCTGAA AGCGACTCAG CCAGTGATAA GGCCTGCAGT 
CCTGCTGGAG TTCCATCCAC CTCCTCACCA CTACCGAGTC CGAGCACTCT GGTGCAGCCG 
TCACAACCTC ATTTCACTCA TATTTCCAGT CCTATTAACG GCACGACAAT GACAAGCCCA 
AATCGACAGT TCAACTTCAG CAAAGTGCGA AAGTCAGCAC TGAACGGATC TTCAACATCA 
TCTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TOX Regulates Growth, DNA Repair, and Genomic Instability in T-cell Acute Lymphoblastic Leukemia.
Cancer discovery7(11)1336-1353(2017 11)
Lobbardi R,Pinder J,Martinez-Pastor B,Theodorou M,Blackburn JS,Abraham BJ,Namiki Y,Mansour M,Abdelfattah NS,Molodtsov A,Alexe G,Toiber D,de Waard M,Jain E,Boukhali M,Lion M,Bhere D,Shah K,Gutierrez A,Stegmaier K,Silverman LB,Sadreyev RI,Asara JM,Oettinger MA,Haas W,Look AT,Young RA,Mostoslavsky R,Dellaire G,Langenau DM

Identification and Expression Analysis of the Complete Family of Zebrafish
Frontiers in cell and developmental biology55(2017)
England SJ,Campbell PC,Banerjee S,Swanson AJ,Lewis KE

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

The Polycomb Group Protein Pcgf1 Is Dispensable in Zebrafish but Involved in Early Growth and Aging.
PloS one11(7)e0158700(2016)
Dupret B,V?lkel P,Le Bourhis X,Angrand PO

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bmi1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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オンラインフォーム

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