BMI1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BMI1
Entrez Gene ID	648
Full Name	BMI1 proto-oncogene, polycomb ring finger
Synonyms	FLVI2/BMI1,PCGF4,RNF51,flvi-2/bmi-1
General protein information	Preferred Names BMI1 proto-oncogene, polycomb ring finger Names polycomb complex protein BMI-1 B lymphoma Mo-MLV insertion region 1 homolog BMI1 polycomb ring finger oncogene BMI1 polycomb ring finger proto-oncogene murine leukemia viral (bmi-1) oncogene homolog polycomb group RING finger protein 4 polycomb group protein Bmi1 ring finger protein 51
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10p12.2
Summary	This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene. [provided by RefSeq, Sep 2015].
Disorder MIM:	164831

mRNA and Protein(s)

mRNA	Protein	Name
NM_005180.8	NP_005171.4	polycomb complex protein BMI-1
NM_005180.9	NP_005171.4	polycomb complex protein BMI-1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	bmi1a	NP_919347.1
Homo sapiens (human)	BMI1	NP_005171.4
Pan troglodytes (chimpanzee)	BMI1	XP_001147119.2

Gene Ontology
Bibliography

Related articles in PubMed

Targeting glioma stem cells through combined BMI1 and EZH2 inhibition.
Jin X, Kim LJY, Wu Q, Wallace LC, Prager BC, Sanvoranart T, Gimple RC, Wang X, Mack SC, Miller TE, Huang P, Valentim CL, Zhou QG, Barnholtz-Sloan JS, Bao S, Sloan AE, Rich JN
Nature medicine23(11)1352-1361(2017 Nov)

Clinical Significance of Bmi1 Expression in Inflammatory Bowel Disease.
Yamada M, Sakurai T, Komeda Y, Nagai T, Kamata K, Minaga K, Yamao K, Takenaka M, Hagiwara S, Matsui S, Watanabe T, Nishida N, Kashida H, Kudo M
Oncology93 Suppl 120-26(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study not only uncovers a novel functional PTMs of Bmi-1 but also reveals a unique oncogenic role of O-GlcNAcylation in prostate cancer.
Title: O-GlcNAcylation modulates Bmi-1 protein stability and potential oncogenic function in prostate cancer.

Given that glioblastomas contain both proneural and mesenchymal GSCs, combined EZH2 and BMI1 targeting proved more effective than either agent alone both in culture and in vivo, suggesting that strategies that simultaneously target multiple epigenetic regulators within glioblastomas may be effective in overcoming therapy resistance caused by intratumoral heterogeneity
Title: Targeting glioma stem cells through combined BMI1 and EZH2 inhibition.

miR-139-5p expression also blocked self-renewal of bladder cancer stem cells by inhibiting Bmi1. In summary, our study supports that miR-139-5p acts as a tumor suppressor in bladder cancer development and suppresses cancer stem cell property of bladder cancer. Our study also suggests that miR-139-5p has the potential to be used as a therapeutic molecule for bladder cancer treatment.
Title: MicroRNA-139-5p inhibits bladder cancer proliferation and self-renewal by targeting the Bmi1 oncogene.

Meta-analysis suggested that Bmi-1 overexpression was correlated with tumor size, poor differentiation, distant metastasis, and worse overall survival in nonsmall cell lung cancer. Therefore, Bmi-1 could be recommended as an efficient prognostic marker for nonsmall cell lung cancer.
Title: Bmi-1 overexpression as an efficient prognostic marker in patients with nonsmall cell lung cancer.

Molecular mechanism studies confirmed that Bmi-1 mediated these effects of miR-495 on MSCs. Taken together, our data demonstrated that miR-495 induced senescence of MSCs may be involved in the pathogenesis of pre-eclampsia (PE)
Title: MiR-495 Promotes Senescence of Mesenchymal Stem Cells by Targeting Bmi-1.

The following BMI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BMI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28592	NM_005180.9 Latest version!	Homo sapiens BMI1 proto-oncogene, polycomb ring finger (BMI1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu28592	NM_005180.8	Homo sapiens BMI1 proto-oncogene, polycomb ring finger (BMI1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BMI1 ( NM_005180.8 ) cDNA ORF clone, Homo sapiens(human) -> NP_005171.4 Homo sapiens BMI1 proto-oncogene, polycomb ring finger (BMI1), mRNA.

Price & Availability↑

CloneID	OHu28592
Clone ID Related Accession (Same CDS sequence)	NM_005180.8 , NM_005180.9
Accession Version	NM_005180.8	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-31
Organism	Homo sapiens(human)
Product	polycomb complex protein BMI-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB225488.1, BC011652.2, BE008203.1 and AI274830.1. On Feb 16, 2011 this sequence version replaced NM_005180.7. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.264561.1, SRR1660805.241287.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATCGAA CAACGAGAAT CAAGATCACT GAGCTAAATC CCCACCTGAT GTGTGTGCTT 
TGTGGAGGGT ACTTCATTGA TGCCACAACC ATAATAGAAT GTCTACATTC CTTCTGTAAA 
ACGTGTATTG TTCGTTACCT GGAGACCAGC AAGTATTGTC CTATTTGTGA TGTCCAAGTT 
CACAAGACCA GACCACTACT GAATATAAGG TCAGATAAAA CTCTCCAAGA TATTGTATAC 
AAATTAGTTC CAGGGCTTTT CAAAAATGAA ATGAAGAGAA GAAGGGATTT TTATGCAGCT 
CATCCTTCTG CTGATGCTGC CAATGGCTCT AATGAAGATA GAGGAGAGGT TGCAGATGAA 
GATAAGAGAA TTATAACTGA TGATGAGATA ATAAGCTTAT CCATTGAATT CTTTGACCAG 
AACAGATTGG ATCGGAAAGT AAACAAAGAC AAAGAGAAAT CTAAGGAGGA GGTGAATGAT 
AAAAGATACT TACGATGCCC AGCAGCAATG ACTGTGATGC ACTTAAGAAA GTTTCTCAGA 
AGTAAAATGG ACATACCTAA TACTTTCCAG ATTGATGTCA TGTATGAGGA GGAACCTTTA 
AAGGATTATT ATACACTAAT GGATATTGCC TACATTTATA CCTGGAGAAG GAATGGTCCA 
CTTCCATTGA AATACAGAGT TCGACCTACT TGTAAAAGAA TGAAGATCAG TCACCAGAGA 
GATGGACTGA CAAATGCTGG AGAACTGGAA AGTGACTCTG GGAGTGACAA GGCCAACAGC 
CCAGCAGGAG GTATTCCCTC CACCTCTTCT TGTTTGCCTA GCCCCAGTAC TCCAGTGCAG 
TCTCCTCATC CACAGTTTCC TCACATTTCC AGTACTATGA ATGGAACCAG CAACAGCCCC 
AGCGGTAACC ACCAATCTTC TTTTGCCAAT AGACCTCGAA AATCATCAGT AAATGGGTCA 
TCAGCAACTT CTTCTGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005171.4
CDS	507..1487
Translation	MHRTTRIKITELNPHLMCVLCGGYFIDATTIIECLHSFCKTCIVRYLETSKYCPICDVQVHKTRPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYAAHPSADAANGSNEDRGEVADEDKRIITDDEIISLSIEFFDQNRLDRKVNKDKEKSKEEVNDKRYLRCPAAMTVMHLRKFLRSKMDIPNTFQIDVMYEEEPLKDYYTLMDIAYIYTWRRNGPLPLKYRVRPTCKRMKISHQRDGLTNAGELESDSGSDKANSPAGGIPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTSNSPSGNHQSSFANRPRKSSVNGSSATSSG

Target ORF information:

RefSeq Version	NM_005180.8
Organism	Homo sapiens(human)
Definition	Homo sapiens BMI1 proto-oncogene, polycomb ring finger (BMI1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005180.8

ORF Insert Sequence:

ATGCATCGAA CAACGAGAAT CAAGATCACT GAGCTAAATC CCCACCTGAT GTGTGTGCTT 
TGTGGAGGGT ACTTCATTGA TGCCACAACC ATAATAGAAT GTCTACATTC CTTCTGTAAA 
ACGTGTATTG TTCGTTACCT GGAGACCAGC AAGTATTGTC CTATTTGTGA TGTCCAAGTT 
CACAAGACCA GACCACTACT GAATATAAGG TCAGATAAAA CTCTCCAAGA TATTGTATAC 
AAATTAGTTC CAGGGCTTTT CAAAAATGAA ATGAAGAGAA GAAGGGATTT TTATGCAGCT 
CATCCTTCTG CTGATGCTGC CAATGGCTCT AATGAAGATA GAGGAGAGGT TGCAGATGAA 
GATAAGAGAA TTATAACTGA TGATGAGATA ATAAGCTTAT CCATTGAATT CTTTGACCAG 
AACAGATTGG ATCGGAAAGT AAACAAAGAC AAAGAGAAAT CTAAGGAGGA GGTGAATGAT 
AAAAGATACT TACGATGCCC AGCAGCAATG ACTGTGATGC ACTTAAGAAA GTTTCTCAGA 
AGTAAAATGG ACATACCTAA TACTTTCCAG ATTGATGTCA TGTATGAGGA GGAACCTTTA 
AAGGATTATT ATACACTAAT GGATATTGCC TACATTTATA CCTGGAGAAG GAATGGTCCA 
CTTCCATTGA AATACAGAGT TCGACCTACT TGTAAAAGAA TGAAGATCAG TCACCAGAGA 
GATGGACTGA CAAATGCTGG AGAACTGGAA AGTGACTCTG GGAGTGACAA GGCCAACAGC 
CCAGCAGGAG GTATTCCCTC CACCTCTTCT TGTTTGCCTA GCCCCAGTAC TCCAGTGCAG 
TCTCCTCATC CACAGTTTCC TCACATTTCC AGTACTATGA ATGGAACCAG CAACAGCCCC 
AGCGGTAACC ACCAATCTTC TTTTGCCAAT AGACCTCGAA AATCATCAGT AAATGGGTCA 
TCAGCAACTT CTTCTGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BMI1 ( NM_005180.9 ) cDNA ORF clone, Homo sapiens(human) -> NP_005171.4 Homo sapiens BMI1 proto-oncogene, polycomb ring finger (BMI1), mRNA.

Price & Availability↑

CloneID	OHu28592
Clone ID Related Accession (Same CDS sequence)	NM_005180.8 , NM_005180.9
Accession Version	NM_005180.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	polycomb complex protein BMI-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL158211.29, DB225488.1, BC011652.2, BE008203.1 and AI274830.1. On Nov 22, 2018 this sequence version replaced NM_005180.8. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.241287.1, SRR1660805.206485.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000376663.8/ ENSP00000365851.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCATCGAA CAACGAGAAT CAAGATCACT GAGCTAAATC CCCACCTGAT GTGTGTGCTT 
TGTGGAGGGT ACTTCATTGA TGCCACAACC ATAATAGAAT GTCTACATTC CTTCTGTAAA 
ACGTGTATTG TTCGTTACCT GGAGACCAGC AAGTATTGTC CTATTTGTGA TGTCCAAGTT 
CACAAGACCA GACCACTACT GAATATAAGG TCAGATAAAA CTCTCCAAGA TATTGTATAC 
AAATTAGTTC CAGGGCTTTT CAAAAATGAA ATGAAGAGAA GAAGGGATTT TTATGCAGCT 
CATCCTTCTG CTGATGCTGC CAATGGCTCT AATGAAGATA GAGGAGAGGT TGCAGATGAA 
GATAAGAGAA TTATAACTGA TGATGAGATA ATAAGCTTAT CCATTGAATT CTTTGACCAG 
AACAGATTGG ATCGGAAAGT AAACAAAGAC AAAGAGAAAT CTAAGGAGGA GGTGAATGAT 
AAAAGATACT TACGATGCCC AGCAGCAATG ACTGTGATGC ACTTAAGAAA GTTTCTCAGA 
AGTAAAATGG ACATACCTAA TACTTTCCAG ATTGATGTCA TGTATGAGGA GGAACCTTTA 
AAGGATTATT ATACACTAAT GGATATTGCC TACATTTATA CCTGGAGAAG GAATGGTCCA 
CTTCCATTGA AATACAGAGT TCGACCTACT TGTAAAAGAA TGAAGATCAG TCACCAGAGA 
GATGGACTGA CAAATGCTGG AGAACTGGAA AGTGACTCTG GGAGTGACAA GGCCAACAGC 
CCAGCAGGAG GTATTCCCTC CACCTCTTCT TGTTTGCCTA GCCCCAGTAC TCCAGTGCAG 
TCTCCTCATC CACAGTTTCC TCACATTTCC AGTACTATGA ATGGAACCAG CAACAGCCCC 
AGCGGTAACC ACCAATCTTC TTTTGCCAAT AGACCTCGAA AATCATCAGT AAATGGGTCA 
TCAGCAACTT CTTCTGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005171.4
CDS	618..1598
Translation	MHRTTRIKITELNPHLMCVLCGGYFIDATTIIECLHSFCKTCIVRYLETSKYCPICDVQVHKTRPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYAAHPSADAANGSNEDRGEVADEDKRIITDDEIISLSIEFFDQNRLDRKVNKDKEKSKEEVNDKRYLRCPAAMTVMHLRKFLRSKMDIPNTFQIDVMYEEEPLKDYYTLMDIAYIYTWRRNGPLPLKYRVRPTCKRMKISHQRDGLTNAGELESDSGSDKANSPAGGIPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTSNSPSGNHQSSFANRPRKSSVNGSSATSSG

Target ORF information:

RefSeq Version	NM_005180.9
Organism	Homo sapiens(human)
Definition	Homo sapiens BMI1 proto-oncogene, polycomb ring finger (BMI1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005180.9

ORF Insert Sequence:

ATGCATCGAA CAACGAGAAT CAAGATCACT GAGCTAAATC CCCACCTGAT GTGTGTGCTT 
TGTGGAGGGT ACTTCATTGA TGCCACAACC ATAATAGAAT GTCTACATTC CTTCTGTAAA 
ACGTGTATTG TTCGTTACCT GGAGACCAGC AAGTATTGTC CTATTTGTGA TGTCCAAGTT 
CACAAGACCA GACCACTACT GAATATAAGG TCAGATAAAA CTCTCCAAGA TATTGTATAC 
AAATTAGTTC CAGGGCTTTT CAAAAATGAA ATGAAGAGAA GAAGGGATTT TTATGCAGCT 
CATCCTTCTG CTGATGCTGC CAATGGCTCT AATGAAGATA GAGGAGAGGT TGCAGATGAA 
GATAAGAGAA TTATAACTGA TGATGAGATA ATAAGCTTAT CCATTGAATT CTTTGACCAG 
AACAGATTGG ATCGGAAAGT AAACAAAGAC AAAGAGAAAT CTAAGGAGGA GGTGAATGAT 
AAAAGATACT TACGATGCCC AGCAGCAATG ACTGTGATGC ACTTAAGAAA GTTTCTCAGA 
AGTAAAATGG ACATACCTAA TACTTTCCAG ATTGATGTCA TGTATGAGGA GGAACCTTTA 
AAGGATTATT ATACACTAAT GGATATTGCC TACATTTATA CCTGGAGAAG GAATGGTCCA 
CTTCCATTGA AATACAGAGT TCGACCTACT TGTAAAAGAA TGAAGATCAG TCACCAGAGA 
GATGGACTGA CAAATGCTGG AGAACTGGAA AGTGACTCTG GGAGTGACAA GGCCAACAGC 
CCAGCAGGAG GTATTCCCTC CACCTCTTCT TGTTTGCCTA GCCCCAGTAC TCCAGTGCAG 
TCTCCTCATC CACAGTTTCC TCACATTTCC AGTACTATGA ATGGAACCAG CAACAGCCCC 
AGCGGTAACC ACCAATCTTC TTTTGCCAAT AGACCTCGAA AATCATCAGT AAATGGGTCA 
TCAGCAACTT CTTCTGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Targeting glioma stem cells through combined BMI1 and EZH2 inhibition.
Nature medicine23(11)1352-1361(2017 Nov)
Jin X,Kim LJY,Wu Q,Wallace LC,Prager BC,Sanvoranart T,Gimple RC,Wang X,Mack SC,Miller TE,Huang P,Valentim CL,Zhou QG,Barnholtz-Sloan JS,Bao S,Sloan AE,Rich JN

Clinical Significance of Bmi1 Expression in Inflammatory Bowel Disease.
Oncology93 Suppl 120-26(2017)
Yamada M,Sakurai T,Komeda Y,Nagai T,Kamata K,Minaga K,Yamao K,Takenaka M,Hagiwara S,Matsui S,Watanabe T,Nishida N,Kashida H,Kudo M

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BMI1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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