c3a.2 cDNA ORF clone, Danio rerio(zebrafish)

The following c3a.2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the c3a.2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa147196 XM_021478306.1
Latest version!
Danio rerio complement component c3a, duplicate 2 (c3a.2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa32081 NM_131243.1
Latest version!
Danio rerio complement component c3a, duplicate 2 (c3a.2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa147196
    Clone ID Related Accession (Same CDS sequence) XM_021478306.1
    Accession Version XM_021478306.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2904bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product complement component c3a, duplicate 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGAGGTGA AGCTGCTGTT TCTCTCTGCT GTTCTTCTCT CCTCAGCGCT TCTCACACTG 
    TGTGACCCGC TGTATGTTCT GATGGCTCCT AATCTGCTGA GAGTGGGTTC ATCAGAGAAC
    GTGTTTGTGG AGGCGCAGGA TTATTCTGGA GAACCCCTAA GAGTGAAGAT CTCTGTGAAG
    AACTTCCCAG CCAAGAATGT GGAGATGATG CAAAAAACAG TGACTCTGAC TGGAGAAAAA
    TATCAGATCC TCACAGACAT AGAGATTCCG GATGATAAAA ACTTCTTCTC TGATGATGAG
    AAGAAGCAGT ATGTGTATCT GCAAGCTCAC TTTCCCTCCG TCACTCTGGA GAAAGTGGTC
    ATGGTGTCTT TTCAGTCTGG ATATTTGTTT GTACAGACGG ACAAGCCCAT CTACACGCCT
    GGCAGCAATG TCCAGTACAG GATTTTTTCT ATGACACCCA ACTTGAAACC ACTCAGTCAG
    CCTGGAATCA CAGTGGATAT CATGAATCCA CAAGGCATCA CGGTAGCATC AGATGCAATA
    AAGGCAGAGA AAGGTGTGAA GTCTTCAATC TACAACATCC CTGATGTTAC TAGTTTAGGG
    ATGTGGAAAG TGGTCACGCG GTACACAAAC ACCCCTCTGA AGACATTTAC TGCAGAGTTT
    GAGGTCAAAG AATACGTGCT GCCGACTTTT GAAGTGAAAT TAACACCCAG TAGTTTTTTC
    TACTACGTGG GAGACAACAA ACAAGAGAAC GACGAGGAAG ACAGTCTGAC AGTTGACATC
    GAAGCCAAGT ATCTGTTTGG AAAGAAGATG GATGGAAATG CATTTGTAGT GTTTGGTGTG
    ATGGATGGTG CGGCGAAAAC AAGTATTGTG AACTCTCTGC AAAAAGTTCC GGTTGTGAAT
    GGAGTAGGAA CTGCAGAACT GACCAGGGAG ATGATCACTA AGACATTTAA AAACATAAAA
    GATCTGGTTG GGAAGTCAAT CTATGTTTCA GTCAACCTTT TAACTGAAAG TGGCAGTGAA
    ATGGTGGAAG CAGAAAAGAG AGGCATTCAG ATTGTGACGT CTCCGTACAC CATCCATTAC
    AAAAGAACAT CACAGTTCTT CAAACCTGGA ATGCCTCTGG GTGTCTCGGT TTACGTAACA
    AATCCTGATG AGACGCCTGC AAAAGATGTG GAAGTGGAGG TGCTGGTTGA TGGAAAGGGA
    GGAGTCAGTG GTAAAACCAG AGACAATGGC ATTGTGAAAG TGAAAGTCAA CACTCCGGGA
    GGAGCCTCCA CATTGAAGAT CACTGCGAAG ACCAAAAATA CAGAATACAC CAGTGAACAG
    CAGGCAGTGA AAACAATGAC AGCTCAGGCC TACAAAACCA AACACGACTC GAAAAACTAC
    CTGCATATCA ACATCGAGTC TGCAGAGTTT GAGATTGGTG ATCAAATGAC GGTTAATCTG
    ATCACTGGAC AAAGTTCAGG AGACAGAGAT CAAGATTACA CATACATGAT CTTGAGTAAA
    GGTCAGATTG TTCTAGCAGA AAGATTTAAG AGGCAAGGAC AAACTCTAGT CACTCTGTCT
    TTAACTATCA CTAAAGAAAT GGTGCCGTCC TTCCGCTTTG TGGCTTATTA CCATGTGGGC
    AAAGATGAGG TGGTGTCTGA TTCAGTCTGG GTCGATGTGA AGGACACATG CATGGGAAAG
    CTCAACGTCG AGGTGAAGGA AAAGAAGAAT ACCTATGAAA CAGGAAATGA TGTCAATCTT
    CACATAACTG GAGATCCTGG AGCCAGAGTT GGTCTGGTGG TGGTTGACAA GGCAGTGCAG
    GTCCTTAACA AGAACAGACT CACTCAGACA AAGGTCTGGG ATGTCATTGA GAAGCATGAT
    ACTGGTTGTA CGGGTGGAGG TGGAAAAGAC AGTATGGGGG TTTTTAGTGA TGCTGGTTTG
    ATTTTTGAGT CCAACACCGC AGGAGGAACT GACACCAGAA CAGTGCCTGA GTGTCCTGCC
    CCTCTGAAGA GAAAACGTAG ATCTGAGAGT CTTCAGACAA TCACTACAAC TCTCGCTGGC
    CAATACACTG AAAAAATCCG GCCATGCTGC TATGATGGGA TGAGGAATAA CAGAATTGGC
    TACACATGTG AACGGCGAGC ATCATACGTC ATAGATGGAG AAGAGTGTGT CAAGGCCTTC
    TTGCACTGCT GCAAAGAGGT GAAAAACCAC AAAGAGAAAG AGACTGAAGA GGAAGAGTTG
    ATTCTGGCTC GCAGTGATGA AGACGAGGAA GGGGATTATA AAGACTATGA TGACATTACA
    TCACGCACGC AGTTTCCTGA GAGCTGGCTT TGGGAGGAGT TTGATCTCTG TGACAAGTGT
    TCAAAACCAA GCAAGGACAA AGTGATTTAC CTAAAAGACT CTATTACTAC CTGGCAGATC
    TTGGCTATCA GCCTCTCACC AACACACGGC ATCTGTGTGG CAGAACCTGA GGAGATCGTC
    GTTTTTAAGA GTTTTTTCAT TGACCTCAAA GTGCCTTACT CAGCTGTACG AGGTGAACAA
    GTGGAAATCA AGGCCATTAT TCACAACTAC ACCCCCAAAC ACCTGAAGAA GGTTCGTGTG
    GAGTTTTTGG AGACAGCAGG TGTTTGCAGT GCTGCCAGTA AGAAAGGGAA ATACAGAACA
    ACAGTAAATG TTGACAAGGA CTCCAGCGTA GCAGTTTCCT TTGTGATCGT TCCAATGACA
    CTGGAAAGTC AGCATATTGA GGTGAAAGCT TCAATATCAG ATTATGAAGA TGGAGTTAGA
    AAGACCCTGA AGGTGGTGTC TGAAGGTGTG CTGACTAAAT TTCGAACTGA AAAACTGGAG
    CTTAACCCAG GCAAAAAAAG GGGAAAAACC CTTAGTTCTT CGATCTGGTA TACCAGCAGA
    TCAGGTTCCA GGCACACCAG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333981.1
    CDS32..2935
    Translation

    Target ORF information:

    RefSeq Version XM_021478306.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio complement component c3a, duplicate 2 (c3a.2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021478306.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGAGGTGA AGCTGCTGTT TCTCTCTGCT GTTCTTCTCT CCTCAGCGCT TCTCACACTG 
    TGTGACCCGC TGTATGTTCT GATGGCTCCT AATCTGCTGA GAGTGGGTTC ATCAGAGAAC
    GTGTTTGTGG AGGCGCAGGA TTATTCTGGA GAACCCCTAA GAGTGAAGAT CTCTGTGAAG
    AACTTCCCAG CCAAGAATGT GGAGATGATG CAAAAAACAG TGACTCTGAC TGGAGAAAAA
    TATCAGATCC TCACAGACAT AGAGATTCCG GATGATAAAA ACTTCTTCTC TGATGATGAG
    AAGAAGCAGT ATGTGTATCT GCAAGCTCAC TTTCCCTCCG TCACTCTGGA GAAAGTGGTC
    ATGGTGTCTT TTCAGTCTGG ATATTTGTTT GTACAGACGG ACAAGCCCAT CTACACGCCT
    GGCAGCAATG TCCAGTACAG GATTTTTTCT ATGACACCCA ACTTGAAACC ACTCAGTCAG
    CCTGGAATCA CAGTGGATAT CATGAATCCA CAAGGCATCA CGGTAGCATC AGATGCAATA
    AAGGCAGAGA AAGGTGTGAA GTCTTCAATC TACAACATCC CTGATGTTAC TAGTTTAGGG
    ATGTGGAAAG TGGTCACGCG GTACACAAAC ACCCCTCTGA AGACATTTAC TGCAGAGTTT
    GAGGTCAAAG AATACGTGCT GCCGACTTTT GAAGTGAAAT TAACACCCAG TAGTTTTTTC
    TACTACGTGG GAGACAACAA ACAAGAGAAC GACGAGGAAG ACAGTCTGAC AGTTGACATC
    GAAGCCAAGT ATCTGTTTGG AAAGAAGATG GATGGAAATG CATTTGTAGT GTTTGGTGTG
    ATGGATGGTG CGGCGAAAAC AAGTATTGTG AACTCTCTGC AAAAAGTTCC GGTTGTGAAT
    GGAGTAGGAA CTGCAGAACT GACCAGGGAG ATGATCACTA AGACATTTAA AAACATAAAA
    GATCTGGTTG GGAAGTCAAT CTATGTTTCA GTCAACCTTT TAACTGAAAG TGGCAGTGAA
    ATGGTGGAAG CAGAAAAGAG AGGCATTCAG ATTGTGACGT CTCCGTACAC CATCCATTAC
    AAAAGAACAT CACAGTTCTT CAAACCTGGA ATGCCTCTGG GTGTCTCGGT TTACGTAACA
    AATCCTGATG AGACGCCTGC AAAAGATGTG GAAGTGGAGG TGCTGGTTGA TGGAAAGGGA
    GGAGTCAGTG GTAAAACCAG AGACAATGGC ATTGTGAAAG TGAAAGTCAA CACTCCGGGA
    GGAGCCTCCA CATTGAAGAT CACTGCGAAG ACCAAAAATA CAGAATACAC CAGTGAACAG
    CAGGCAGTGA AAACAATGAC AGCTCAGGCC TACAAAACCA AACACGACTC GAAAAACTAC
    CTGCATATCA ACATCGAGTC TGCAGAGTTT GAGATTGGTG ATCAAATGAC GGTTAATCTG
    ATCACTGGAC AAAGTTCAGG AGACAGAGAT CAAGATTACA CATACATGAT CTTGAGTAAA
    GGTCAGATTG TTCTAGCAGA AAGATTTAAG AGGCAAGGAC AAACTCTAGT CACTCTGTCT
    TTAACTATCA CTAAAGAAAT GGTGCCGTCC TTCCGCTTTG TGGCTTATTA CCATGTGGGC
    AAAGATGAGG TGGTGTCTGA TTCAGTCTGG GTCGATGTGA AGGACACATG CATGGGAAAG
    CTCAACGTCG AGGTGAAGGA AAAGAAGAAT ACCTATGAAA CAGGAAATGA TGTCAATCTT
    CACATAACTG GAGATCCTGG AGCCAGAGTT GGTCTGGTGG TGGTTGACAA GGCAGTGCAG
    GTCCTTAACA AGAACAGACT CACTCAGACA AAGGTCTGGG ATGTCATTGA GAAGCATGAT
    ACTGGTTGTA CGGGTGGAGG TGGAAAAGAC AGTATGGGGG TTTTTAGTGA TGCTGGTTTG
    ATTTTTGAGT CCAACACCGC AGGAGGAACT GACACCAGAA CAGTGCCTGA GTGTCCTGCC
    CCTCTGAAGA GAAAACGTAG ATCTGAGAGT CTTCAGACAA TCACTACAAC TCTCGCTGGC
    CAATACACTG AAAAAATCCG GCCATGCTGC TATGATGGGA TGAGGAATAA CAGAATTGGC
    TACACATGTG AACGGCGAGC ATCATACGTC ATAGATGGAG AAGAGTGTGT CAAGGCCTTC
    TTGCACTGCT GCAAAGAGGT GAAAAACCAC AAAGAGAAAG AGACTGAAGA GGAAGAGTTG
    ATTCTGGCTC GCAGTGATGA AGACGAGGAA GGGGATTATA AAGACTATGA TGACATTACA
    TCACGCACGC AGTTTCCTGA GAGCTGGCTT TGGGAGGAGT TTGATCTCTG TGACAAGTGT
    TCAAAACCAA GCAAGGACAA AGTGATTTAC CTAAAAGACT CTATTACTAC CTGGCAGATC
    TTGGCTATCA GCCTCTCACC AACACACGGC ATCTGTGTGG CAGAACCTGA GGAGATCGTC
    GTTTTTAAGA GTTTTTTCAT TGACCTCAAA GTGCCTTACT CAGCTGTACG AGGTGAACAA
    GTGGAAATCA AGGCCATTAT TCACAACTAC ACCCCCAAAC ACCTGAAGAA GGTTCGTGTG
    GAGTTTTTGG AGACAGCAGG TGTTTGCAGT GCTGCCAGTA AGAAAGGGAA ATACAGAACA
    ACAGTAAATG TTGACAAGGA CTCCAGCGTA GCAGTTTCCT TTGTGATCGT TCCAATGACA
    CTGGAAAGTC AGCATATTGA GGTGAAAGCT TCAATATCAG ATTATGAAGA TGGAGTTAGA
    AAGACCCTGA AGGTGGTGTC TGAAGGTGTG CTGACTAAAT TTCGAACTGA AAAACTGGAG
    CTTAACCCAG GCAAAAAAAG GGGAAAAACC CTTAGTTCTT CGATCTGGTA TACCAGCAGA
    TCAGGTTCCA GGCACACCAG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa32081
    Clone ID Related Accession (Same CDS sequence) NM_131243.1
    Accession Version NM_131243.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-21
    Organism Danio rerio(zebrafish)
    Product complement component c3a, duplicate 2 precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB235997.1. On Nov 23, 2005 this sequence version replaced XM_688299.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB235997.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505386, SAMEA4476730 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGGTGA AGCTGCTGTT TCTCTCTGCT GTTCTTCTCT CCTCAGCGCT CCTCACACTG 
    TGTGACCCGC TGTATGTTCT GATGGCTCCT AATCTGCTGA GAGTGGGTTC ATCAGAGAAC
    GTGTTTGTGG AGGCGCAGGA TTACTCTGGA GAACCCCTAA GAGTGAAGAT CTCTGTGAAG
    AACTTCCCAG CCAAGAATGT GGAGATGATG CAAAAAACAG TGACTCTGAC TGGAGAAAAA
    TATCAGATCC TCACAGACAT AGAGATTCCG GATGATAAAA ACTTTTTCTC TGATGATGAG
    AAGAAGCAGT ATGTGTATCT GCAAGCTCAC TTTCCCTCCG TCACTCTGGA GAAAGTGGTC
    ATGGTGTCTT TTCAGTCTGG ATATTTGTTT GTACAGACGG ACAAGCCCAT CTACACGCCT
    GGCAGCAATG TCCAGTACAG GATTTTCTCT ATGACACCCA ACTTGAAACC ACTCAGTCAG
    CCTGGAATCA CAGTGGATAT CATGAATCCA CAAGGCATCA CGGTAGCATC AGACGCAATA
    AAGGCAGAGA AAGGTGTGAA GTCTTCAATC TACAACATCC CTGATGTTAC TAGTTTGGGG
    ATGTGGAAAG TGGTCACGCG GTACACAAAC ACCCCTCTGA AGACATTTAC TGCAGAGTTT
    GAGGTCAAAG AATACGTGCT GCCGACTTTT GAAGTGAAAT TAACACCCAG TAGTTTTTTC
    TACTACGTGG GAGACAACAA ACAAGAGAAC GATGAGGAAG ACAGTCTGAC AGTTGACATC
    GAAGCCAAGT ATCTGTTTGG AAAGAAGATG GATGGAAATG CATTTGTAGT GTTTGGTGTG
    ATGGATGGTG CGGCGAAAAC AAGTATTGTG AACTCTCTGC AAAAAGTTCC GGTTGTGAAT
    GGAGTAGGAA CTGCAGAATT GACCAGGGAG ATGATCACTA AGACATTTAA AAACATAAAA
    GATCTGGTTG GGAAATCAAT CTACGTTTCA GTCAACCTTT TAACTGAAAG TGGCAGTGAA
    ATGGTGGAAG CAGAAAAGAG AGGCATTCAG ATTGTGACGT CTCCGTACAC CATCCATTAC
    AAAAGAACAT CACAGTTCTT CAAACCTGGA ATGCCCCTGG GTGTCTCGGT TTACGTAACA
    AATCCTGATG AGACGCCTGC AAAAGATGTG GAAGTGGAGG TGCTGGTTGA TGGAAAGGGA
    GGAGTCAGTG GTAAAACCAG AGACAATGGC ATTGCGAAAG TGAAAGTCAA CACTCCGGGA
    GGAGCCTCCA CATTGAAGAT CACTGCGAAG ACCAAAAATA CAGAATACAC CAGTGAACAG
    CAGGCAGTGA AAACAATGAC AGCTCAGGCC TACAAAACCA AACACGACTC GAAAAACTAC
    CTGCATATCA ACATCGAGTC TGCAGAGTTT GAGATTGGTG ATCAAATGAC GGTTAATCTG
    ATCACTGGAC AAAGTTCAGG AGACAGAGAT CAAGATTACA CATACATGAT CTTGAGTAAA
    GGTCAGATTG TTCTAGCAGA AAGATTTAAG AGGCAAGGAC AAACTCTAGT CACTCTGTCT
    TTAACTATCA CTAAAGAAAT GGTGCCGTCC TTCCGCTTTG TGGCTTATTA CCATGTGGGC
    AAAGATGAGG TGGTGTCTGA TTCAGTCTGG GTCGATGTGA AGGACACATG CATGGGAAAG
    CTCAACGTCG AGGTGAAGGA AAAGAAGAAT ACCTATGAAA CAGGAAATGA TGTCAATCTT
    CACATAACTG GAGATCCTGG AGCCAGAGTT GGTCTGGTGG TGGTTGACAA GGCAGTGCAG
    GTCCTTAACA AGAACAGACT CACTCAGTCG AAGGTCTGGG ATGTCATTGA GAAGCATGAT
    ACTGGTTGTA CGGGTGGAGG TGGAAAAGAC AGTATGGGGG TTTTTAGTGA TGCTGGTTTG
    ATTTTTGAGT CCAACACCGC AGGAGGAACT GACACCAGAA CAGTGCCTGA GTGTCCTGCC
    CCTCTGAAGA GAAAACGGAG ATCTGAGAGT CTTCAGACAA TCACTACAAC TCTCGCTGGC
    CAATACACTG AAAAAATCCG GCCATGCTGC TATGACGGGA TGAGGAATAA CAGAATTGGC
    TACACATGTG AACGGCGAGC ATCATACGTC ATAGATGGAG AAGAGTGTGT CAAGGCCTTC
    TTGCACTGCT GCAAAGAGGT GAAAAACCAC AAAGAGAAAG AGACTGAAGA GGAAGAGCTG
    ATTCTGGCTC GTAGTGATGA AGACGAGGAA GGGGATTATA AAGACTATGA TGACATTACA
    TCACGCACGC AGTTTCCTGA GAGCTGGCTT TGGGAGGAGT TTGATCTCTG TGACAAGTGT
    TCAAAACCAA GCAAGGACAA AGTGATTTAC CTAAAAGACT CTATTACTAC CTGGCAGATC
    TTGGCTATCA GCCTCTCACC AACACACGGC ATCTGTGTGG CAGAACCTGA GGAGATCGTC
    GTTTTTAAGA GTTTTTTCAT TGACCTCAAA GTGCCTTACT CAGCTGTACG AGGTGAACAA
    GTGGAAATCA AGGCCATTAT TCACAACTAC ACCCCCAAAC ACCTGAAGAA GGTTCGTGTG
    GAGTTTTTGG AGACAGCAGG GGTTTGCAGT GCTGCCAGTA AGAAAGGGAA ATACAGAACA
    ACAGTAAATG TTGACAAGGA CTCCAGCGTA GCAGTTTCCT TTGTGATCGT TCCAATGACA
    CTGGAAAGTC AGCATATTGA GGTGAAAGCT TCAATATCAG ATTATGAAGA TGGAGTTAGA
    AAGACCCTGA AGGTGGTGTC TGAAGGTGTG CTGACTAAAT TTCGAACTGA AAAACTGGAG
    CTTAACCCAG GCAAAAAAGG GGAAAAACCC TTAGTTCTTC GATCTGGTAT ACCAGCAGAT
    CAGGTTCCAG GCACACCAGT TAACTCGTAC ATCACAGTTA CTGGTGAAGA AATCAGTCAG
    TCGATTGAGC AGGCCATCAA TGGAAGCTTC ATGGGAGAAC TTATCATCCA GCCTCATGGA
    TGTGGAGAGC AGAATATGGT CACCATGACT TTACCTTTCA TTGCCACTCA TTATCTGGAC
    AACACCAATC AGTGGGAATA TGTTGGCATT GACCGCCGTA AAGTTGCCAT TAACCATATC
    AAAACAGGTT ATGAAGGGCA GCTAAAGTTT CGAAAACAAG ATGGATCTTA CGCTCTCTGG
    ATTGAAACAG ACAGTAGCAC ATGGCTGACA GCATATGTGG CAAAAGTCTT CGCCATGGCA
    AGTAAGCTTA TTGCTATAGA GGAAAATGTG ATCTGCAGCG CCCTCAAGTG GCTAGTTATG
    CACAAACAAC TACCAGATGG CTCTTTTAAA GAGGATAAAC CTGTATATCA CCGGGAAATT
    ATGGGTGGCA TAGAAGGCCA AAACACTGAT GTCTCTCTGA CAGCCTTTGT CTTGATTGCC
    ATGCAAGAAG GCAAAGAGAT CTGTGGGGGA GCAGTTGGAA GTCTGCATGA AAGTATAAGG
    AAGTCAGTTG CTTTCTTGAA AAATCAGCTC ACAAAACTGA CCAATCCTTA TGCAGCTGCG
    TTGACGTCCT ACGCCCTGGC CAATGCCGAC AGTCTTGATA AAGAAATCTT GATGAAGCAT
    TCCACCAAAG GAAACGATGG AAGGTTCTGG AATGTCCCTG GATGGCATTA TCACTCAATA
    GAGGCGACGG CCTATGCTGT GCTGGCACTC GTGAAGGCCA AAGACTATGA TACAGCAGGA
    GAGGCAGTGC ATTGGCTGGC CAAACAAAAA TCTAGTCATG GTGGTTATGA CACTACACAG
    GTGACCATCA TGGTTTTCCA GGCTGTGGCA GAGTACCGTG CTCAGGTGCA AGACCTAAAA
    AACTTTGACC TGAATGTGGA GCTGGTTGTG CCAGGAAGAC GAAAAACTGA CAGATGGACC
    ATTCAAAGAA GTAATGTGCA TCTTACGCGG TCAGGCAGTG TGGATATGAA TAAGGACTTC
    ACTGTGATTG CTAAAGGAAC CGGATCAGCC ATTCTCTCAG TTCTGACGCT GTACTACGCC
    AGACCGTTTG AGAAGAAGAC TGACTGTACT TTCTTTGATC TGAGTGTCAA ATTGGATAAA
    ATCCCTGAAA CAAAACCAGG AACCAATGGA ACGTACAACT TCATCCTGGA TTTCTTCTAC
    AAAAACAAAA CAAATGACGC CACCATGACG ATTCTGGACA TTGGCTTGCC AACAGGATTT
    GATATCGATG AAGCTGATCT TAAACAGTTG TCCACAGGGA AGGAGAGATA CATTCAGAAA
    TATGAAAAGA ATAAAGTGCT CTCAGAGCGA GGATCCCTCA TCATCTACTT AAACAAGGTT
    TCTCATAAGG AATCAGAAAC TCTTGTCTTC AGAATGCACA AGACTTTTAA CGTGGGTTTG
    CTTCAACCTG CTTCCGTCAC CATATATGAG TATTACTCAC CCGATGCCCG CTGTAAAAAG
    TACTACCACC CTGAAAGGGA AGATTCCGCC CTATACAGAC TGTGTAAAGG AGACCTGTGT
    GTGTGTGCTG AAGAAAACTG CAGCTACCAG AAGAAAAACC AAGTCAATGA GTCTGATCGC
    TTGATTAAAG CCTGTGAACC CCATCTGGAT TACATTTATA AAGCGAAATT AGTGAAGATG
    GACCTGAAAC AAGACTCCGA TATCTATCAC ATGAAGGTGG AGAAGACGGT GAAAGAAGGA
    ACTGATATGG ATGTTGGAGG GAAAGTGAAA CCATTTTTGG CTCGTCCCAG TTGCCGAGTG
    AGTTTAGGAC TGGTTGAAGG AAAGTCGTAT CTCATCATGG GAAACTCTGT TGATCTGCCG
    ACCCTTGGGG GAAGTCTGCA GTACATCATA GGAGAGCAGA CCTGGATTGA ATACTGGCCT
    ACAAGTGACG AAAGTAAATC TGCTGAACAC AGAGATCGCT ACATCGGCAT GACTGCAGTC
    GAGAATATGT TTACCACAGG AGGATGTTTG ACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_571318.1
    CDS22..4977
    Translation

    Target ORF information:

    RefSeq Version NM_131243.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio complement component c3a, duplicate 2 (c3a.2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_131243.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGGTGA AGCTGCTGTT TCTCTCTGCT GTTCTTCTCT CCTCAGCGCT CCTCACACTG 
    TGTGACCCGC TGTATGTTCT GATGGCTCCT AATCTGCTGA GAGTGGGTTC ATCAGAGAAC
    GTGTTTGTGG AGGCGCAGGA TTACTCTGGA GAACCCCTAA GAGTGAAGAT CTCTGTGAAG
    AACTTCCCAG CCAAGAATGT GGAGATGATG CAAAAAACAG TGACTCTGAC TGGAGAAAAA
    TATCAGATCC TCACAGACAT AGAGATTCCG GATGATAAAA ACTTTTTCTC TGATGATGAG
    AAGAAGCAGT ATGTGTATCT GCAAGCTCAC TTTCCCTCCG TCACTCTGGA GAAAGTGGTC
    ATGGTGTCTT TTCAGTCTGG ATATTTGTTT GTACAGACGG ACAAGCCCAT CTACACGCCT
    GGCAGCAATG TCCAGTACAG GATTTTCTCT ATGACACCCA ACTTGAAACC ACTCAGTCAG
    CCTGGAATCA CAGTGGATAT CATGAATCCA CAAGGCATCA CGGTAGCATC AGACGCAATA
    AAGGCAGAGA AAGGTGTGAA GTCTTCAATC TACAACATCC CTGATGTTAC TAGTTTGGGG
    ATGTGGAAAG TGGTCACGCG GTACACAAAC ACCCCTCTGA AGACATTTAC TGCAGAGTTT
    GAGGTCAAAG AATACGTGCT GCCGACTTTT GAAGTGAAAT TAACACCCAG TAGTTTTTTC
    TACTACGTGG GAGACAACAA ACAAGAGAAC GATGAGGAAG ACAGTCTGAC AGTTGACATC
    GAAGCCAAGT ATCTGTTTGG AAAGAAGATG GATGGAAATG CATTTGTAGT GTTTGGTGTG
    ATGGATGGTG CGGCGAAAAC AAGTATTGTG AACTCTCTGC AAAAAGTTCC GGTTGTGAAT
    GGAGTAGGAA CTGCAGAATT GACCAGGGAG ATGATCACTA AGACATTTAA AAACATAAAA
    GATCTGGTTG GGAAATCAAT CTACGTTTCA GTCAACCTTT TAACTGAAAG TGGCAGTGAA
    ATGGTGGAAG CAGAAAAGAG AGGCATTCAG ATTGTGACGT CTCCGTACAC CATCCATTAC
    AAAAGAACAT CACAGTTCTT CAAACCTGGA ATGCCCCTGG GTGTCTCGGT TTACGTAACA
    AATCCTGATG AGACGCCTGC AAAAGATGTG GAAGTGGAGG TGCTGGTTGA TGGAAAGGGA
    GGAGTCAGTG GTAAAACCAG AGACAATGGC ATTGCGAAAG TGAAAGTCAA CACTCCGGGA
    GGAGCCTCCA CATTGAAGAT CACTGCGAAG ACCAAAAATA CAGAATACAC CAGTGAACAG
    CAGGCAGTGA AAACAATGAC AGCTCAGGCC TACAAAACCA AACACGACTC GAAAAACTAC
    CTGCATATCA ACATCGAGTC TGCAGAGTTT GAGATTGGTG ATCAAATGAC GGTTAATCTG
    ATCACTGGAC AAAGTTCAGG AGACAGAGAT CAAGATTACA CATACATGAT CTTGAGTAAA
    GGTCAGATTG TTCTAGCAGA AAGATTTAAG AGGCAAGGAC AAACTCTAGT CACTCTGTCT
    TTAACTATCA CTAAAGAAAT GGTGCCGTCC TTCCGCTTTG TGGCTTATTA CCATGTGGGC
    AAAGATGAGG TGGTGTCTGA TTCAGTCTGG GTCGATGTGA AGGACACATG CATGGGAAAG
    CTCAACGTCG AGGTGAAGGA AAAGAAGAAT ACCTATGAAA CAGGAAATGA TGTCAATCTT
    CACATAACTG GAGATCCTGG AGCCAGAGTT GGTCTGGTGG TGGTTGACAA GGCAGTGCAG
    GTCCTTAACA AGAACAGACT CACTCAGTCG AAGGTCTGGG ATGTCATTGA GAAGCATGAT
    ACTGGTTGTA CGGGTGGAGG TGGAAAAGAC AGTATGGGGG TTTTTAGTGA TGCTGGTTTG
    ATTTTTGAGT CCAACACCGC AGGAGGAACT GACACCAGAA CAGTGCCTGA GTGTCCTGCC
    CCTCTGAAGA GAAAACGGAG ATCTGAGAGT CTTCAGACAA TCACTACAAC TCTCGCTGGC
    CAATACACTG AAAAAATCCG GCCATGCTGC TATGACGGGA TGAGGAATAA CAGAATTGGC
    TACACATGTG AACGGCGAGC ATCATACGTC ATAGATGGAG AAGAGTGTGT CAAGGCCTTC
    TTGCACTGCT GCAAAGAGGT GAAAAACCAC AAAGAGAAAG AGACTGAAGA GGAAGAGCTG
    ATTCTGGCTC GTAGTGATGA AGACGAGGAA GGGGATTATA AAGACTATGA TGACATTACA
    TCACGCACGC AGTTTCCTGA GAGCTGGCTT TGGGAGGAGT TTGATCTCTG TGACAAGTGT
    TCAAAACCAA GCAAGGACAA AGTGATTTAC CTAAAAGACT CTATTACTAC CTGGCAGATC
    TTGGCTATCA GCCTCTCACC AACACACGGC ATCTGTGTGG CAGAACCTGA GGAGATCGTC
    GTTTTTAAGA GTTTTTTCAT TGACCTCAAA GTGCCTTACT CAGCTGTACG AGGTGAACAA
    GTGGAAATCA AGGCCATTAT TCACAACTAC ACCCCCAAAC ACCTGAAGAA GGTTCGTGTG
    GAGTTTTTGG AGACAGCAGG GGTTTGCAGT GCTGCCAGTA AGAAAGGGAA ATACAGAACA
    ACAGTAAATG TTGACAAGGA CTCCAGCGTA GCAGTTTCCT TTGTGATCGT TCCAATGACA
    CTGGAAAGTC AGCATATTGA GGTGAAAGCT TCAATATCAG ATTATGAAGA TGGAGTTAGA
    AAGACCCTGA AGGTGGTGTC TGAAGGTGTG CTGACTAAAT TTCGAACTGA AAAACTGGAG
    CTTAACCCAG GCAAAAAAGG GGAAAAACCC TTAGTTCTTC GATCTGGTAT ACCAGCAGAT
    CAGGTTCCAG GCACACCAGT TAACTCGTAC ATCACAGTTA CTGGTGAAGA AATCAGTCAG
    TCGATTGAGC AGGCCATCAA TGGAAGCTTC ATGGGAGAAC TTATCATCCA GCCTCATGGA
    TGTGGAGAGC AGAATATGGT CACCATGACT TTACCTTTCA TTGCCACTCA TTATCTGGAC
    AACACCAATC AGTGGGAATA TGTTGGCATT GACCGCCGTA AAGTTGCCAT TAACCATATC
    AAAACAGGTT ATGAAGGGCA GCTAAAGTTT CGAAAACAAG ATGGATCTTA CGCTCTCTGG
    ATTGAAACAG ACAGTAGCAC ATGGCTGACA GCATATGTGG CAAAAGTCTT CGCCATGGCA
    AGTAAGCTTA TTGCTATAGA GGAAAATGTG ATCTGCAGCG CCCTCAAGTG GCTAGTTATG
    CACAAACAAC TACCAGATGG CTCTTTTAAA GAGGATAAAC CTGTATATCA CCGGGAAATT
    ATGGGTGGCA TAGAAGGCCA AAACACTGAT GTCTCTCTGA CAGCCTTTGT CTTGATTGCC
    ATGCAAGAAG GCAAAGAGAT CTGTGGGGGA GCAGTTGGAA GTCTGCATGA AAGTATAAGG
    AAGTCAGTTG CTTTCTTGAA AAATCAGCTC ACAAAACTGA CCAATCCTTA TGCAGCTGCG
    TTGACGTCCT ACGCCCTGGC CAATGCCGAC AGTCTTGATA AAGAAATCTT GATGAAGCAT
    TCCACCAAAG GAAACGATGG AAGGTTCTGG AATGTCCCTG GATGGCATTA TCACTCAATA
    GAGGCGACGG CCTATGCTGT GCTGGCACTC GTGAAGGCCA AAGACTATGA TACAGCAGGA
    GAGGCAGTGC ATTGGCTGGC CAAACAAAAA TCTAGTCATG GTGGTTATGA CACTACACAG
    GTGACCATCA TGGTTTTCCA GGCTGTGGCA GAGTACCGTG CTCAGGTGCA AGACCTAAAA
    AACTTTGACC TGAATGTGGA GCTGGTTGTG CCAGGAAGAC GAAAAACTGA CAGATGGACC
    ATTCAAAGAA GTAATGTGCA TCTTACGCGG TCAGGCAGTG TGGATATGAA TAAGGACTTC
    ACTGTGATTG CTAAAGGAAC CGGATCAGCC ATTCTCTCAG TTCTGACGCT GTACTACGCC
    AGACCGTTTG AGAAGAAGAC TGACTGTACT TTCTTTGATC TGAGTGTCAA ATTGGATAAA
    ATCCCTGAAA CAAAACCAGG AACCAATGGA ACGTACAACT TCATCCTGGA TTTCTTCTAC
    AAAAACAAAA CAAATGACGC CACCATGACG ATTCTGGACA TTGGCTTGCC AACAGGATTT
    GATATCGATG AAGCTGATCT TAAACAGTTG TCCACAGGGA AGGAGAGATA CATTCAGAAA
    TATGAAAAGA ATAAAGTGCT CTCAGAGCGA GGATCCCTCA TCATCTACTT AAACAAGGTT
    TCTCATAAGG AATCAGAAAC TCTTGTCTTC AGAATGCACA AGACTTTTAA CGTGGGTTTG
    CTTCAACCTG CTTCCGTCAC CATATATGAG TATTACTCAC CCGATGCCCG CTGTAAAAAG
    TACTACCACC CTGAAAGGGA AGATTCCGCC CTATACAGAC TGTGTAAAGG AGACCTGTGT
    GTGTGTGCTG AAGAAAACTG CAGCTACCAG AAGAAAAACC AAGTCAATGA GTCTGATCGC
    TTGATTAAAG CCTGTGAACC CCATCTGGAT TACATTTATA AAGCGAAATT AGTGAAGATG
    GACCTGAAAC AAGACTCCGA TATCTATCAC ATGAAGGTGG AGAAGACGGT GAAAGAAGGA
    ACTGATATGG ATGTTGGAGG GAAAGTGAAA CCATTTTTGG CTCGTCCCAG TTGCCGAGTG
    AGTTTAGGAC TGGTTGAAGG AAAGTCGTAT CTCATCATGG GAAACTCTGT TGATCTGCCG
    ACCCTTGGGG GAAGTCTGCA GTACATCATA GGAGAGCAGA CCTGGATTGA ATACTGGCCT
    ACAAGTGACG AAAGTAAATC TGCTGAACAC AGAGATCGCT ACATCGGCAT GACTGCAGTC
    GAGAATATGT TTACCACAGG AGGATGTTTG ACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.