C3 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	C3
Entrez Gene ID	396370
Full Name	complement component 3
Synonyms	C3d
General protein information	Preferred Names complement component 3 Names complement C3 complement C3 alpha chain complement component 3d
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_205405.3	NP_990736.3	complement C3
NM_205405.2	NP_990736.2	complement C3 precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	c3c	XP_005168460.1
Danio rerio (zebrafish)	LOC100331492	XP_002660624.3
Danio rerio (zebrafish)	c3a	NP_571317.1
Xenopus tropicalis (tropical clawed frog)	c3	XP_004919147.1
Xenopus tropicalis (tropical clawed frog)	LOC100492293	XP_002940095.2
Xenopus tropicalis (tropical clawed frog)	LOC100497914	XP_002941061.2
Canis lupus familiaris (dog)	C3	XP_005633285.1
Bos taurus (cattle)	C3	NP_001035559.2
Mus musculus (house mouse)	C3	NP_033908.2
Rattus norvegicus (Norway rat)	C3	NP_058690.2
Xenopus tropicalis (tropical clawed frog)	LOC100492966	XP_002940099.2
Homo sapiens (human)	C3	NP_000055.2
Gallus gallus (chicken)	C3	NP_990736.1
Danio rerio (zebrafish)	LOC100331428	XP_002660623.3
Xenopus tropicalis (tropical clawed frog)	LOC100491968	XP_004919148.1
Pan troglodytes (chimpanzee)	C3	XP_512318.4
	C3	XP_001091921.2
Danio rerio (zebrafish)	zgc:103710	NP_001008582.3
Danio rerio (zebrafish)	c3b	NP_571318.1
Xenopus tropicalis (tropical clawed frog)	LOC100492134	XP_002940094.2
Xenopus tropicalis (tropical clawed frog)	LOC100497762	XP_004919229.1

Gene Ontology
Bibliography

Related articles in PubMed

Splenic gene expression profiling in White Leghorn layer inoculated with the Salmonella enterica serovar Enteritidis.
Wu G, Liu L, Qi Y, Sun Y, Yang N, Xu G, Zhou H, Li X
Animal genetics46(6)617-26(2015 Dec)

Complement anaphylatoxin C3a is a potent inducer of embryonic chick retina regeneration.
Haynes T, Luz-Madrigal A, Reis ES, Echeverri Ruiz NP, Grajales-Esquivel E, Tzekou A, Tsonis PA, Lambris JD, Del Rio-Tsonis K
Nature communications42312(2013)

Cloning of a gene fragment encoding chicken complement component C3d with expression and immunogenicity of Newcastle disease virus F gene-C3d fusion protein.
Liu D, Niu ZX
Avian pathology : journal of the W.V.P.A37(5)477-85(2008 Oct)

Anti-ischemia/reperfusion of C1 inhibitor in myocardial cell injury via regulation of local myocardial C3 activity.
Fu J, Lin G, Zeng B, Wu Z, Wu Y, Chu H, Qin G, Liang G, Li J, Gan X, Yu X, Li C, Liu D
Biochemical and biophysical research communications350(1)162-8(2006 Nov)

Receptor-mediated transport and deposition of complement component C3 into developing chicken oocytes.
Recheis B, Rumpler H, Schneider WJ, Nimpf J
Cellular and molecular life sciences : CMLS62(16)1871-80(2005 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

C3a is sufficient to induce complete regeneration of the embryonic chick retina from stem/progenitor cells present in the eye, independent of fibroblast growth factor receptor signaling.
Title: Complement anaphylatoxin C3a is a potent inducer of embryonic chick retina regeneration.

Complement C1 Inactivator Proteins, in addition to inhibition of the systemic complement activation, prevents myocardial cell injury via a direct inhibitory role in the local myocardial C3 activity
Title: Anti-ischemia/reperfusion of C1 inhibitor in myocardial cell injury via regulation of local myocardial C3 activity.

C3 is transported into oocytes by LR8-mediated endocytosis.
Title: Receptor-mediated transport and deposition of complement component C3 into developing chicken oocytes.

The following C3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the C3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa153255	NM_205405.3 Latest version!	Gallus gallus complement component 3 (C3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OGa86378	NM_205405.2 Latest version!	Gallus gallus complement component 3 (C3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

C3 ( NM_205405.3 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990736.3 Gallus gallus complement component 3 (C3), mRNA.

Price & Availability↑

CloneID	OGa153255
Clone ID Related Accession (Same CDS sequence)	NM_205405.3
Accession Version	NM_205405.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-30
Organism	Gallus gallus(chicken)
Product	complement C3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000789.1. On May 31, 2018 this sequence version replaced NM_205405.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: U16848.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201357, SAMEA2201358 [ECO:0000348] ##Evidence-Data-END##

ATGGGCTGCT GCTGCTGCCC CTCCTGCTCG GCGTTCTGCT GCTCCATGCG GTCCCCACCT 
GCACAGATGG TGACCATGGT GACCCCGGCG GTGCTGCGGT TGGACACGGA CGAGAAGGTG 
GTGTTGGAGG CTCCGGGTCT GTCCGCCCCC ACCGAGGCCA ACATCCTGGT GCAGGATTTC 
CCCCAAAAGC GCAAAGTCCT CTTCCAGGTC CGCAAGCAGC TCAACCCCGC AGAGGGGATG 
ATGGCCATCG CCACCGTCAA GGTGCCGGTG AAGCTGCTGC CGCCGGTGGT GGGGAAGCAC 
TTTGTCTCGG TGGTGGCGCG GGTGGGACAG GTGACCCTGG AGAAGGTGCT GTTGGTGTCA 
CTGCAGAGCG GCCACATCTT CCTGCAGACC GACAAACCCA TCTACACCCC CGGCTCCACC 
GTGCTCTGCC GTCTCTTCGC CCTCAGCCAC TTCATGCAGC CTCTGCTGAA GACGGTGATC 
GTGGAGGTCA AGACACCCGA CAACGTCATC ATCAAGCAAG TGCCCGTGTC CTCCCCCATG 
AGGAATGGGA TCTTCTCCAT CAACCACAAC CTGCCGGAGG TGGTCAGCCT GGGGACATGG 
ACTATCACGG CCAAATTTGA AGACTCGCAG GACCAGGTCT TCAGCACACA ATTTGAAGTC 
AAGGAATACG TGCTGCCGAG CTTTGAGGTC ACCCTGGACC CGCAGGAGAA GTTCCTCTAC 
ATTGACCGGC AGGAGGATTT CCGGGTGACC ATCACAGCCA GGTACCTGTA TGGGAAGAAT 
CTGCAGGGGA CCGCCTTCGT CCTCTTCGGT GTGGTGGTGG ACGACGAGAA AAAGACCATC 
CCCCAGTCCC TGCAGCGCGT CAAGGTGACC GATGGGGACG GGGAGGCCGT GCTGCCCATG 
GCCATGCTGC GGCAGCGCTT CGCCAACCTC CAGGAGCTGG TGGGACACTC TCTCTACGTC 
ACCGTCACCG TCCTCACCGA GTCAGGCAGT GACATGGTGG AGGCACAGCG CAGCGGCATC 
CGCATAGTGA CGTCCCCATA CACCATCCAC TTCACCCACA CCCCCAAGTA CTTCAAGCCA 
GGGATGCCCT TCGATCTGAC GGTTTATGTC ACCAACCCCG ATAATTCCCC GGCTCCGCGC 
ATCCCCGTCA AGGCCGACAA CTTCCAGGGC CTCGTCTCCA CGCAGCGAGA TGGCACAGCC 
AAGCTGGTCC TCAACATGCC AGCCAACAAG AACTCCGTCC CCATCACTGT GAGGACAGAC 
CAGAAGGATC TGCCCCCGGA GCGCCAGGCC TCGCGGCAGA TAGTGGCCGA GGCGTATCAA 
AGCCAGGGGA ACTCTGGCAA CTACCTTCAC CTGGCAGTGG GTGCCAGCCA GGTGCAGCCC 
GGGGACAACC TCCCCATCAA CTTCCATCTC AAGAGCAACA GAGATGACGT CCGCAAATCC 
GTTTCCTACT TCACCTACCT GATCCTGAGC AAGGGGCACA TTGTCCACGT GGGACGGCAG 
CCAAGGGAAG GTGACCAGAG CCTGGTCACG ATGTCGCTTC CCGTGACGGC CAACCTCATC 
CCTTCCTTCC GTATCGTGGC CTACTACCAC GTGAAGCCTG GCGAGATCAT TGCTGACTCC 
GTCTGGGTCG ATGTCAAGGA CACCTGCATG GGCAGTCTGG TGGTGAGGGG AGCGTCGGAG 
GCTGACAATC GTGTGCATGA GCCAAGGACC CCCATGCGGC TGCACATCGA GGGCGACCAC 
AAAGCCCACG TGGGGCTGGT GGCTGTGGAC AAGGCTGTCT ATGTCCTCAA CAAGAACAAA 
CTCACTCAGA GTAAGGTGTG GGACACAGTG GAGAACAGCG ACATCGGCTG CACGCCGGGC 
AGTGGGAGGA ACCACGTGGG GGTCTTCGCC GATGCCGGCC TCAGCCTGAC TTCAAACGTG 
AACATCAACA CGGAGCAGAG AAGTGAGGTC CAGTGTGCAA AGCCTGCAAA ACGCAAGCGC 
CGCTCCGTGA GGCTCATCAA GCACAAGGGC ACCAAGATGG CCGAGTACAG CGACAAGAAC 
CTGCGCAAGT GCTGTGAGGA CGGCATGAAG GAAAACCTGA TGGGCTACAG CTGCGAGAAA 
CGGGCCACCT ACGTCCTCGA TGGCAAAGCC TGCACCGAAG CCTTCCTCAG CTGCTGCCTC 
TACATCAAGG GCATCCGCGA TGAGGAGCGC GAGCTGCAAT ACGAGCTGGC TCGAAGTGAG 
GTGGATGACG CCTTCCTGAG TGATGAAGAC ATCACCTCAC GGAGCCTCTT TCCAGAGAGC 
TGGCTATGGC AGGTGGAGGA GCTGACAGAA CCACCCAATG AACAGGGCAT CTCCATGAAG 
ACGCTGCCCA TATACCTGAA AGACTCCATC ACCACCTGGG AGGTTCTGGC TGTCAGTATC 
TCTGAGAACA AGGGTCTGTG CGTGGCCGAC CCCTATGAGA TTACGGTGAT GAAGGAGTTC 
TTCATTGACC TGCGCCTGCC CTACTCGGCA GTGAGGAACG AGCAGGTGGA GGTCCGCGCC 
ATTCTCTACA ACTACTGGAC GAACAAGATC AAGGTGCGCG TGGAGCTGAT GTACAACCCA 
GCCCTGTGCA GCGCATCCAC CACCAAGACG CGCTACCAGC AGATCTTCCA ACTGGAACCT 
CAGTCGTCGC ACGCCGTGCC CTTCGTCATC GTGCCCCTGC AGCTGGGGCA GCATGACGTC 
GAGGTGAAGG CAGCTGTCTG GAACAGCTTT GTGTCTGATG GCGTCAAGAA GAAGCTCAGA 
GTGGTGCCTG AAGGGATGAG GCTGGAGAAG ACAGTGAAGA TAGTGGAGCT TGACCCAAAG 
ACGCTGGGAA ATAACGGTGT GCAAGAAGTG AAGGTGAAAG CAGCAAACCT CTCTGACATC 
GTCCCCAACA CTGAGTCGGA GACCAAAGTC AGCATTCAAG GCAACCCTGT GTCCATCCTG 
GTGGAGAAAG CCACCGATGG GACCAAGCTG AAACACCTCA TTGTGACCCC CTCGGGCTGT 
GGGGAGCAGA ACATGATTGG GATGACGCCC ACCGTCATTG CTGTCCACTA CCTGGACAGC 
ACAATGCAGT GGGAGACCTT CGGTATCAAC CGCCGCACTG AAGCCATCGA ACTGATTAAA 
AAGGGTTACA CCCAACAACT TGCATACCGC AAAGAAGACG GCTCATTTGC TGCCTTCACT 
ACCCGCCCAT CGAGCACCTG GTTGACAGCC TACGTGGCCA AGGTCTTTGC CATGGCCATC 
AACATGGTGG ACATCAAGCC GGAGGTGGTT TGTGGAGCCA TCAAATGGCT CATTCTGGAG 
AAGCAACAGC CAGATGGGCT TTTCCAAGAA GACGCTCCTG TCATCCACAA GGAGATGGTG 
GGAGGCTACC ACGGTGCTGA GCCCAGTGTG TCCCTGACAG CCTTCGTCCT CTCCGCGCTG 
CAGGAATCCC AGAAGATCTG CAAGAACTAC GTGAAAAGCC TGGATGGGAG CATTGCCAAA 
GCCTCCGATT ACCTCTCCCG GAAATACCAA TCTTTGACTC GACCCTACAC GGTGGCCCTG 
ACCTCCTACG CCCTGGCCCT AACGGGGAAA CTCAACAGCG AGAAAGTCCT GATGAAGTTC 
TCCAAAGATG GCACCCACTG GGCGGAACGC AACGCCCACA CCTACAACAT CGAGGGGACG 
TCCTACGCTC TGCTGGCGCT GCTGCAGATG GAGAAGGCCG AGCTGACGGG GCCGGTGGTC 
CGCTGGTTGG CCCAGCAGAA CTACTTCGGT GGTGGCTACG GATCCACCCA GGCCACCATC 
CTGGTGTTCC AGGCTCTGGC TCAGTACCAC GTGGCGCTGC CGCGGCAGCT GGAGCTCAAC 
CTGGACGTGT CGGTGCTGCT GCCGCGCCGC GCCAACGCCA TCACCTACCG CATCGAGAAC 
AACAACGCGC TGGTGGCACG CTCAGCTGAG ACCAAGCTGA ACGAGGACTT CACTGTGAAA 
GCAGAGGGCA CTGGCAAGGG GACAATGACA GTGGTGACCG TCTACAACGC CAAAGTGCCC 
GAGAAGGAAA ACAAGTGTGA CAACTTCGAC CTGCGGGTCA GCGTGGAGGA CGTGAAGGCG 
GGCCGGGAGC TGGAAGGGGT CATCCGCTCT GTCAAGATCA CCATCTGCAC CAGGTTCCTG 
GACACCGTGG ATGCCACCAT GTCCATCCTT GATATCTCCA TGCTCACCGG CTTCTCCCCT 
GACGTCCAGG ACCTGAAGAG TCTCTCGGAG GGAGTGGAGA GGTACATTTC CAAATTTGAG 
ATCGACCACG CGCTGTCGAA CCGCAGCAAC CTCATCATCT ACCTGGACAA GGTCTCCCAC 
CAAGTGGAGG AGTGCATCGC CTTCAGGGCC CACCAGCACT TCCAGGTGGG ACTGATCCAG 
CCCGCCTCCG TCACTGTCTA CAGCTACTAC AAGATCGATG ACCGCTGCAC CCGCTTCTAC 
CACCCGGACA AGGCTGGTGG GCAGCTGAGG AAGATCTGCC ATGGGGAAGT GTGTCGCTGC 
GCTGAAGAAA ACTGCTTCAT ACGGGTGAAG AAGGACAATC CCATCACAGT CAATGAGCGC 
ATCGACCTTG CCTGCAAGCC AGGGGTGGAC TATGTGTACA AAGTGAAGGT GGTGGCAACA 
GAGGAGACGC CATCCCACGA CAACTACATC ATGTCCATCC TCACCGTCAT CAAGATGGGC 
ACTGATGAGA ACCCAGGTGG GAGCAACCGG ACCTTCGTGA GCCATAAGCA GTGCCGGGAT 
GCATTGAGTC TCCAGAAGGG ACAGGACTAC CTGGTGTGGG GGCTGGCGTC CGACCTGTGG 
GTCACCGGCA GCCGCTTCTC CTACCTCATC AGCAAGGACA CGTGGCTGGA AGCGTGGCCC 
TTGGAGGAGT CGTGCCAGGA TGCCGACCTG CAGCCGCTCT GCCAGGACTT CACCGAGTTC 
TCCGACAATA TGGTCTTGTT TGGGTGCCCC ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990736.3
CDS	26..4981
Translation	MGCCCCPSCSAFCCSMRSPPAQMVTMVTPAVLRLDTDEKVVLEAPGLSAPTEANILVQDFPQKRKVLFQVRKQLNPAEGMMAIATVKVPVKLLPPVVGKHFVSVVARVGQVTLEKVLLVSLQSGHIFLQTDKPIYTPGSTVLCRLFALSHFMQPLLKTVIVEVKTPDNVIIKQVPVSSPMRNGIFSINHNLPEVVSLGTWTITAKFEDSQDQVFSTQFEVKEYVLPSFEVTLDPQEKFLYIDRQEDFRVTITARYLYGKNLQGTAFVLFGVVVDDEKKTIPQSLQRVKVTDGDGEAVLPMAMLRQRFANLQELVGHSLYVTVTVLTESGSDMVEAQRSGIRIVTSPYTIHFTHTPKYFKPGMPFDLTVYVTNPDNSPAPRIPVKADNFQGLVSTQRDGTAKLVLNMPANKNSVPITVRTDQKDLPPERQASRQIVAEAYQSQGNSGNYLHLAVGASQVQPGDNLPINFHLKSNRDDVRKSVSYFTYLILSKGHIVHVGRQPREGDQSLVTMSLPVTANLIPSFRIVAYYHVKPGEIIADSVWVDVKDTCMGSLVVRGASEADNRVHEPRTPMRLHIEGDHKAHVGLVAVDKAVYVLNKNKLTQSKVWDTVENSDIGCTPGSGRNHVGVFADAGLSLTSNVNINTEQRSEVQCAKPAKRKRRSVRLIKHKGTKMAEYSDKNLRKCCEDGMKENLMGYSCEKRATYVLDGKACTEAFLSCCLYIKGIRDEERELQYELARSEVDDAFLSDEDITSRSLFPESWLWQVEELTEPPNEQGISMKTLPIYLKDSITTWEVLAVSISENKGLCVADPYEITVMKEFFIDLRLPYSAVRNEQVEVRAILYNYWTNKIKVRVELMYNPALCSASTTKTRYQQIFQLEPQSSHAVPFVIVPLQLGQHDVEVKAAVWNSFVSDGVKKKLRVVPEGMRLEKTVKIVELDPKTLGNNGVQEVKVKAANLSDIVPNTESETKVSIQGNPVSILVEKATDGTKLKHLIVTPSGCGEQNMIGMTPTVIAVHYLDSTMQWETFGINRRTEAIELIKKGYTQQLAYRKEDGSFAAFTTRPSSTWLTAYVAKVFAMAINMVDIKPEVVCGAIKWLILEKQQPDGLFQEDAPVIHKEMVGGYHGAEPSVSLTAFVLSALQESQKICKNYVKSLDGSIAKASDYLSRKYQSLTRPYTVALTSYALALTGKLNSEKVLMKFSKDGTHWAERNAHTYNIEGTSYALLALLQMEKAELTGPVVRWLAQQNYFGGGYGSTQATILVFQALAQYHVALPRQLELNLDVSVLLPRRANAITYRIENNNALVARSAETKLNEDFTVKAEGTGKGTMTVVTVYNAKVPEKENKCDNFDLRVSVEDVKAGRELEGVIRSVKITICTRFLDTVDATMSILDISMLTGFSPDVQDLKSLSEGVERYISKFEIDHALSNRSNLIIYLDKVSHQVEECIAFRAHQHFQVGLIQPASVTVYSYYKIDDRCTRFYHPDKAGGQLRKICHGEVCRCAEENCFIRVKKDNPITVNERIDLACKPGVDYVYKVKVVATEETPSHDNYIMSILTVIKMGTDENPGGSNRTFVSHKQCRDALSLQKGQDYLVWGLASDLWVTGSRFSYLISKDTWLEAWPLEESCQDADLQPLCQDFTEFSDNMVLFGCPT

Target ORF information:

RefSeq Version	NM_205405.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus complement component 3 (C3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205405.3

ORF Insert Sequence:

ATGGGCTGCT GCTGCTGCCC CTCCTGCTCG GCGTTCTGCT GCTCCATGCG GTCCCCACCT 
GCACAGATGG TGACCATGGT GACCCCGGCG GTGCTGCGGT TGGACACGGA CGAGAAGGTG 
GTGTTGGAGG CTCCGGGTCT GTCCGCCCCC ACCGAGGCCA ACATCCTGGT GCAGGATTTC 
CCCCAAAAGC GCAAAGTCCT CTTCCAGGTC CGCAAGCAGC TCAACCCCGC AGAGGGGATG 
ATGGCCATCG CCACCGTCAA GGTGCCGGTG AAGCTGCTGC CGCCGGTGGT GGGGAAGCAC 
TTTGTCTCGG TGGTGGCGCG GGTGGGACAG GTGACCCTGG AGAAGGTGCT GTTGGTGTCA 
CTGCAGAGCG GCCACATCTT CCTGCAGACC GACAAACCCA TCTACACCCC CGGCTCCACC 
GTGCTCTGCC GTCTCTTCGC CCTCAGCCAC TTCATGCAGC CTCTGCTGAA GACGGTGATC 
GTGGAGGTCA AGACACCCGA CAACGTCATC ATCAAGCAAG TGCCCGTGTC CTCCCCCATG 
AGGAATGGGA TCTTCTCCAT CAACCACAAC CTGCCGGAGG TGGTCAGCCT GGGGACATGG 
ACTATCACGG CCAAATTTGA AGACTCGCAG GACCAGGTCT TCAGCACACA ATTTGAAGTC 
AAGGAATACG TGCTGCCGAG CTTTGAGGTC ACCCTGGACC CGCAGGAGAA GTTCCTCTAC 
ATTGACCGGC AGGAGGATTT CCGGGTGACC ATCACAGCCA GGTACCTGTA TGGGAAGAAT 
CTGCAGGGGA CCGCCTTCGT CCTCTTCGGT GTGGTGGTGG ACGACGAGAA AAAGACCATC 
CCCCAGTCCC TGCAGCGCGT CAAGGTGACC GATGGGGACG GGGAGGCCGT GCTGCCCATG 
GCCATGCTGC GGCAGCGCTT CGCCAACCTC CAGGAGCTGG TGGGACACTC TCTCTACGTC 
ACCGTCACCG TCCTCACCGA GTCAGGCAGT GACATGGTGG AGGCACAGCG CAGCGGCATC 
CGCATAGTGA CGTCCCCATA CACCATCCAC TTCACCCACA CCCCCAAGTA CTTCAAGCCA 
GGGATGCCCT TCGATCTGAC GGTTTATGTC ACCAACCCCG ATAATTCCCC GGCTCCGCGC 
ATCCCCGTCA AGGCCGACAA CTTCCAGGGC CTCGTCTCCA CGCAGCGAGA TGGCACAGCC 
AAGCTGGTCC TCAACATGCC AGCCAACAAG AACTCCGTCC CCATCACTGT GAGGACAGAC 
CAGAAGGATC TGCCCCCGGA GCGCCAGGCC TCGCGGCAGA TAGTGGCCGA GGCGTATCAA 
AGCCAGGGGA ACTCTGGCAA CTACCTTCAC CTGGCAGTGG GTGCCAGCCA GGTGCAGCCC 
GGGGACAACC TCCCCATCAA CTTCCATCTC AAGAGCAACA GAGATGACGT CCGCAAATCC 
GTTTCCTACT TCACCTACCT GATCCTGAGC AAGGGGCACA TTGTCCACGT GGGACGGCAG 
CCAAGGGAAG GTGACCAGAG CCTGGTCACG ATGTCGCTTC CCGTGACGGC CAACCTCATC 
CCTTCCTTCC GTATCGTGGC CTACTACCAC GTGAAGCCTG GCGAGATCAT TGCTGACTCC 
GTCTGGGTCG ATGTCAAGGA CACCTGCATG GGCAGTCTGG TGGTGAGGGG AGCGTCGGAG 
GCTGACAATC GTGTGCATGA GCCAAGGACC CCCATGCGGC TGCACATCGA GGGCGACCAC 
AAAGCCCACG TGGGGCTGGT GGCTGTGGAC AAGGCTGTCT ATGTCCTCAA CAAGAACAAA 
CTCACTCAGA GTAAGGTGTG GGACACAGTG GAGAACAGCG ACATCGGCTG CACGCCGGGC 
AGTGGGAGGA ACCACGTGGG GGTCTTCGCC GATGCCGGCC TCAGCCTGAC TTCAAACGTG 
AACATCAACA CGGAGCAGAG AAGTGAGGTC CAGTGTGCAA AGCCTGCAAA ACGCAAGCGC 
CGCTCCGTGA GGCTCATCAA GCACAAGGGC ACCAAGATGG CCGAGTACAG CGACAAGAAC 
CTGCGCAAGT GCTGTGAGGA CGGCATGAAG GAAAACCTGA TGGGCTACAG CTGCGAGAAA 
CGGGCCACCT ACGTCCTCGA TGGCAAAGCC TGCACCGAAG CCTTCCTCAG CTGCTGCCTC 
TACATCAAGG GCATCCGCGA TGAGGAGCGC GAGCTGCAAT ACGAGCTGGC TCGAAGTGAG 
GTGGATGACG CCTTCCTGAG TGATGAAGAC ATCACCTCAC GGAGCCTCTT TCCAGAGAGC 
TGGCTATGGC AGGTGGAGGA GCTGACAGAA CCACCCAATG AACAGGGCAT CTCCATGAAG 
ACGCTGCCCA TATACCTGAA AGACTCCATC ACCACCTGGG AGGTTCTGGC TGTCAGTATC 
TCTGAGAACA AGGGTCTGTG CGTGGCCGAC CCCTATGAGA TTACGGTGAT GAAGGAGTTC 
TTCATTGACC TGCGCCTGCC CTACTCGGCA GTGAGGAACG AGCAGGTGGA GGTCCGCGCC 
ATTCTCTACA ACTACTGGAC GAACAAGATC AAGGTGCGCG TGGAGCTGAT GTACAACCCA 
GCCCTGTGCA GCGCATCCAC CACCAAGACG CGCTACCAGC AGATCTTCCA ACTGGAACCT 
CAGTCGTCGC ACGCCGTGCC CTTCGTCATC GTGCCCCTGC AGCTGGGGCA GCATGACGTC 
GAGGTGAAGG CAGCTGTCTG GAACAGCTTT GTGTCTGATG GCGTCAAGAA GAAGCTCAGA 
GTGGTGCCTG AAGGGATGAG GCTGGAGAAG ACAGTGAAGA TAGTGGAGCT TGACCCAAAG 
ACGCTGGGAA ATAACGGTGT GCAAGAAGTG AAGGTGAAAG CAGCAAACCT CTCTGACATC 
GTCCCCAACA CTGAGTCGGA GACCAAAGTC AGCATTCAAG GCAACCCTGT GTCCATCCTG 
GTGGAGAAAG CCACCGATGG GACCAAGCTG AAACACCTCA TTGTGACCCC CTCGGGCTGT 
GGGGAGCAGA ACATGATTGG GATGACGCCC ACCGTCATTG CTGTCCACTA CCTGGACAGC 
ACAATGCAGT GGGAGACCTT CGGTATCAAC CGCCGCACTG AAGCCATCGA ACTGATTAAA 
AAGGGTTACA CCCAACAACT TGCATACCGC AAAGAAGACG GCTCATTTGC TGCCTTCACT 
ACCCGCCCAT CGAGCACCTG GTTGACAGCC TACGTGGCCA AGGTCTTTGC CATGGCCATC 
AACATGGTGG ACATCAAGCC GGAGGTGGTT TGTGGAGCCA TCAAATGGCT CATTCTGGAG 
AAGCAACAGC CAGATGGGCT TTTCCAAGAA GACGCTCCTG TCATCCACAA GGAGATGGTG 
GGAGGCTACC ACGGTGCTGA GCCCAGTGTG TCCCTGACAG CCTTCGTCCT CTCCGCGCTG 
CAGGAATCCC AGAAGATCTG CAAGAACTAC GTGAAAAGCC TGGATGGGAG CATTGCCAAA 
GCCTCCGATT ACCTCTCCCG GAAATACCAA TCTTTGACTC GACCCTACAC GGTGGCCCTG 
ACCTCCTACG CCCTGGCCCT AACGGGGAAA CTCAACAGCG AGAAAGTCCT GATGAAGTTC 
TCCAAAGATG GCACCCACTG GGCGGAACGC AACGCCCACA CCTACAACAT CGAGGGGACG 
TCCTACGCTC TGCTGGCGCT GCTGCAGATG GAGAAGGCCG AGCTGACGGG GCCGGTGGTC 
CGCTGGTTGG CCCAGCAGAA CTACTTCGGT GGTGGCTACG GATCCACCCA GGCCACCATC 
CTGGTGTTCC AGGCTCTGGC TCAGTACCAC GTGGCGCTGC CGCGGCAGCT GGAGCTCAAC 
CTGGACGTGT CGGTGCTGCT GCCGCGCCGC GCCAACGCCA TCACCTACCG CATCGAGAAC 
AACAACGCGC TGGTGGCACG CTCAGCTGAG ACCAAGCTGA ACGAGGACTT CACTGTGAAA 
GCAGAGGGCA CTGGCAAGGG GACAATGACA GTGGTGACCG TCTACAACGC CAAAGTGCCC 
GAGAAGGAAA ACAAGTGTGA CAACTTCGAC CTGCGGGTCA GCGTGGAGGA CGTGAAGGCG 
GGCCGGGAGC TGGAAGGGGT CATCCGCTCT GTCAAGATCA CCATCTGCAC CAGGTTCCTG 
GACACCGTGG ATGCCACCAT GTCCATCCTT GATATCTCCA TGCTCACCGG CTTCTCCCCT 
GACGTCCAGG ACCTGAAGAG TCTCTCGGAG GGAGTGGAGA GGTACATTTC CAAATTTGAG 
ATCGACCACG CGCTGTCGAA CCGCAGCAAC CTCATCATCT ACCTGGACAA GGTCTCCCAC 
CAAGTGGAGG AGTGCATCGC CTTCAGGGCC CACCAGCACT TCCAGGTGGG ACTGATCCAG 
CCCGCCTCCG TCACTGTCTA CAGCTACTAC AAGATCGATG ACCGCTGCAC CCGCTTCTAC 
CACCCGGACA AGGCTGGTGG GCAGCTGAGG AAGATCTGCC ATGGGGAAGT GTGTCGCTGC 
GCTGAAGAAA ACTGCTTCAT ACGGGTGAAG AAGGACAATC CCATCACAGT CAATGAGCGC 
ATCGACCTTG CCTGCAAGCC AGGGGTGGAC TATGTGTACA AAGTGAAGGT GGTGGCAACA 
GAGGAGACGC CATCCCACGA CAACTACATC ATGTCCATCC TCACCGTCAT CAAGATGGGC 
ACTGATGAGA ACCCAGGTGG GAGCAACCGG ACCTTCGTGA GCCATAAGCA GTGCCGGGAT 
GCATTGAGTC TCCAGAAGGG ACAGGACTAC CTGGTGTGGG GGCTGGCGTC CGACCTGTGG 
GTCACCGGCA GCCGCTTCTC CTACCTCATC AGCAAGGACA CGTGGCTGGA AGCGTGGCCC 
TTGGAGGAGT CGTGCCAGGA TGCCGACCTG CAGCCGCTCT GCCAGGACTT CACCGAGTTC 
TCCGACAATA TGGTCTTGTT TGGGTGCCCC ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C3 ( NM_205405.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990736.2 Gallus gallus complement component 3 (C3), mRNA.

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CloneID	OGa86378
Clone ID Related Accession (Same CDS sequence)	NM_205405.2
Accession Version	NM_205405.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-05
Organism	Gallus gallus(chicken)
Product	complement C3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN04001210.1 and U16848.1. On Jan 6, 2017 this sequence version replaced NM_205405.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

ATGGGGCTGC TGCTGCTGCC CCTCCTGCTC GGCGTTCTGC TGCTCCATGC GGTCCCCACA 
CCTGCACAGA TGGTGACCAT GGTGACCCCG GCGGTGCTGC GGTTGGACAC GGACGAGAAG 
GTGGTGTTGG AGGCTCCGGG TCTGTCCGCC CCCACCGAGG CCAACATCCT GGTGCAGGAT 
TTCCCCCAAA AGCGCAAAGT CCTCTTCCAG GTCCGCAAGC AGCTCAACCC CGCAGAGGGG 
ATGATGGCCA TCGCCACCGT CAAGGTGCCG GTGAAGCTGC TGCCGCCGGT GGTGGGGAAG 
CACTTTGTCT CGGTGGTGGC GCGGGTGGGA CAGGTGACCC TGGAGAAGGT GCTGTTGGTG 
TCACTGCAGA GCGGCCACAT CTTCCTGCAG ACCGACAAAC CCATCTACAC CCCCGGCTCC 
ACCGTGCTCT GCCGTCTCTT CGCCCTCAGC CACTTCATGC AGCCTCTGCT GAAGACGGTG 
ATCGTGGAGG TCAAGACACC CGACAACGTC ATCATCAAGC AAGTGCCCGT GTCCTCCCCC 
ATGAGGAATG GGATCTTCTC CATCAACCAC AACCTGCCGG AGGTGGTCAG CCTGGGGACA 
TGGACTATCA CGGCCAAATT TGAAGACTCG CAGGACCAGG TCTTCAGCAC ACAATTTGAA 
GTCAAGGAAT ACGTGCTGCC GAGCTTTGAG GTCACCCTGG ACCCGCAGGA GAAGTTCCTC 
TACATTGACC GGCAGGAGGA TTTCCGGGTG ACCATCACAG CCAGGTACCT GTATGGGAAG 
AATCTGCAGG GGACCGCCTT CGTCCTCTTC GGTGTGGTGG TGGACGACGA GAAAAAGACC 
ATCCCCCAGT CCCTGCAGCG CGTCAAGGTG ACCGATGGGG ACGGGGAGGC CGTGCTGCCC 
ATGGCCATGC TGCGGCAGCG CTTCGCCAAC CTCCAGGAGC TGGTGGGACA CTCTCTCTAC 
GTCACCGTCA CCGTCCTCAC CGAGTCAGGC AGTGACATGG TGGAGGCACA GCGCAGCGGC 
ATCCGCATAG TGACGTCCCC ATACACCATC CACTTCACCC ACACCCCCAA GTACTTCAAG 
CCAGGGATGC CCTTCGATCT GACGGTTTAT GTCACCAACC CCGATAATTC CCCGGCTCCG 
CGCATCCCCG TCAAGGCCGA CAACTTCCAG GGCCTCGTCT CCACGCAGCG AGATGGCACA 
GCCAAGCTGG TCCTCAACAT GCCAGCCAAC AAGAACTCCG TCCCCATCAC TGTGAGGACA 
GACCAGAAGG ATCTGCCCCC GGAGCGCCAG GCCTCGCGGC AGATAGTGGC CGAGGCGTAT 
CAAAGCCAGG GGAACTCTGG CAACTACCTT CACCTGGCAG TGGGTGCCAG CCAGGTGCAG 
CCCGGGGACA ACCTCCCCAT CAACTTCCAT CTCAAGAGCA ACAGAGATGA CGTCCGCAAA 
TCCGTTTCCT ACTTCACCTA CCTGATCCTG AGCAAGGGGC ACATTGTCCA CGTGGGACGG 
CAGCCAAGGG AAGGTGACCA GAGCCTGGTC ACGATGTCGC TTCCCGTGAC GGCCAACCTC 
ATCCCTTCCT TCCGTATCGT GGCCTACTAC CACGTGAAGC CTGGCGAGAT CATTGCTGAC 
TCCGTCTGGG TCGATGTCAA GGACACCTGC ATGGGCAGTC TGGTGGTGAG GGGAGCGTCG 
GAGGCTGACA ATCGTGTGCA TGAGCCAAGG ACCCCCATGC GGCTGCACAT CGAGGGCGAC 
CACAAAGCCC ACGTGGGGCT GGTGGCTGTG GACAAGGCTG TCTATGTCCT CAACAAGAAC 
AAACTCACTC AGAGTAAGGT GTGGGACACA GTGGAGAACA GCGACATCGG CTGCACGCCG 
GGCAGTGGGA GGAACCACGT GGGGGTCTTC GCCGATGCCG GCCTCAGCCT GACTTCAAAC 
GTGAACATCA ACACGGAGCA GAGAAGTGAG GTCCAGTGTG CAAAGCCTGC AAAACGCAAG 
CGCCGCTCCG TGAGGCTCAT CAAGCACAAG GGCACCAAGA TGGCCGAGTA CAGCGACAAG 
AACCTGCGCA AGTGCTGTGA GGACGGCATG AAGGAAAACC TGATGGGCTA CAGCTGCGAG 
AAACGGGCCA CCTACGTCCT CGATGGCAAA GCCTGCACCG AAGCCTTCCT CAGCTGCTGC 
CTCTACATCA AGGGCATCCG CGATGAGGAG CGCGAGCTGC AATACGAGCT GGCTCGAAGT 
GAGGTGGATG ACGCCTTCCT GAGTGATGAA GACATCACCT CACGGAGCCT CTTTCCAGAG 
AGCTGGCTAT GGCAGGTGGA GGAGCTGACA GAACCACCCA ATGAACAGGG CATCTCCATG 
AAGACGCTGC CCATATACCT GAAAGACTCC ATCACCACCT GGGAGGTTCT GGCTGTCAGT 
ATCTCTGAGA ACAAGGGTCT GTGCGTGGCC GACCCCTATG AGATTACGGT GATGAAGGAG 
TTCTTCATTG ACCTGCGCCT GCCCTACTCG GCAGTGAGGA ACGAGCAGGT GGAGGTCCGC 
GCCATTCTCT ACAACTACTG GACGAACAAG ATCAAGGTGC GCGTGGAGCT GATGTACAAC 
CCAGCCCTGT GCAGCGCATC CACCACCAAG ACGCGCTACC AGCAGATCTT CCAACTGGAA 
CCTCAGTCGT CGCACGCCGT GCCCTTCGTC ATCGTGCCCC TGCAGCTGGG GCAGCATGAC 
GTCGAGGTGA AGGCAGCTGT CTGGAACAGC TTTGTGTCTG ATGGCGTCAA GAAGAAGCTC 
AGAGTGGTGC CTGAAGGGAT GAGGCTGGAG AAGACAGTGA AGATAGTGGA GCTTGACCCA 
AAGACGCTGG GAAATAACGG TGTGCAAGAA GTGAAGGTGA AAGCAGCAAA CCTCTCTGAC 
ATCGTCCCCA ACACTGAGTC GGAGACCAAA GTCAGCATTC AAGGCAACCC TGTGTCCATC 
CTGGTGGAGA AAGCCACCGA TGGGACCAAG CTGAAACACC TCATTGTGAC CCCCTCGGGC 
TGTGGGGAGC AGAACATGAT TGGGATGACG CCCACCGTCA TTGCTGTCCA CTACCTGGAC 
AGCACAATGC AGTGGGAGAC CTTCGGTATC AACCGCCGCA CTGAAGCCAT CGAACTGATT 
AAAAAGGGTT ACACCCAACA ACTTGCATAC CGCAAAGAAG ACGGCTCATT TGCTGCCTTC 
ACTACCCGCC CATCGAGCAC CTGGTTGACA GCCTACGTGG CCAAGGTCTT TGCCATGGCC 
ATCAACATGG TGGACATCAA GCCGGAGGTG GTTTGTGGAG CCATCAAATG GCTCATTCTG 
GAGAAGCAAC AGCCAGATGG GCTTTTCCAA GAAGACGCTC CTGTCATCCA CAAGGAGATG 
GTGGGAGGCT ACCACGGTGC TGAGCCCAGT GTGTCCCTGA CAGCCTTCGT CCTCTCCGCG 
CTGCAGGAAT CCCAGAAGAT CTGCAAGAAC TACGTGAAAA GCCTGGATGG GAGCATTGCC 
AAAGCCTCCG ATTACCTCTC CCGGAAATAC CAATCTTTGA CTCGACCCTA CACGGTGGCC 
CTGACCTCCT ACGCCCTGGC CCTAACGGGG AAACTCAACA GCGAGAAAGT CCTGATGAAG 
TTCTCCAAAG ATGGCACCCA CTGGGCGGAA CGCAACGCCC ACACCTACAA CATCGAGGGG 
ACGTCCTACG CTCTGCTGGC GCTGCTGCAG ATGGAGAAGG CCGAGCTGAC GGGGCCGGTG 
GTCCGCTGGT TGGCCCAGCA GAACTACTTC GGTGGTGGCT ACGGATCCAC CCAGGCCACC 
ATCCTGGTGT TCCAGGCTCT GGCTCAGTAC CACGTGGCGC TGCCGCGGCA GCTGGAGCTC 
AACCTGGACG TGTCGGTGCT GCTGCCGCGC CGCGCCAACG CCATCACCTA CCGCATCGAG 
AACAACAACG CGCTGGTGGC ACGCTCAGCT GAGACCAAGC TGAACGAGGA CTTCACTGTG 
AAAGCAGAGG GCACTGGCAA GGGGACAATG ACAGTGGTGA CCGTCTACAA CGCCAAAGTG 
CCCGAGAAGG AAAACAAGTG TGACAACTTC GACCTGCGGG TCAGCGTGGA GGACGTGAAG 
GCGGGCCGGG AGCTGGAAGG GGTCATCCGC TCTGTCAAGA TCACCATCTG CACCAGGTTC 
CTGGACACCG TGGATGCCAC CATGTCCATC CTTGATATCT CCATGCTCAC CGGCTTCTCC 
CCTGACGTCC AGGACCTGAA GAGTCTCTCG GAGGGAGTGG AGAGGTACAT TTCCAAATTT 
GAGATCGACC ACGCGCTGTC GAACCGCAGC AACCTCATCA TCTACCTGGA CAAGGTCTCC 
CACCAAGTGG AGGAGTGCAT CGCCTTCAGG GCCCACCAGC ACTTCCAGGT GGGACTGATC 
CAGCCCGCCT CCGTCATTGT CTACAGCTAC TACAAGATCG ATGACCGCTG CACCCGCTTC 
TACCACCCGG ACAAGGCTGG TGGGCAGCTG AGGAAGATCT GCCATGGGGA AGTGTGCTGC 
GCTGAAGAAA ACTGCTTCAT ACGGGTGAAG AAGGACAATC CCATCACAGT CAATGAGCGC 
ATCGACCTTG CCTGCAAGCC AGGGGTGGAC TATGTGTACA AAGTGAAGGT GGTGGCAACA 
GAGGAGACGC CATCCCACGA CAACTACATC ATGTCCATCC TCACCGTCAT CAAGATGGGC 
ACTGATGAGA ACCCAGGTGG GAGCAACCGG ACCTTCGTGA GCCATAAGCA GTGCCGGGAT 
GCATTGAGTC TCCAGAAGGG ACAGGACTAC CTGGTGTGGG GGCTGGCGTC CGACCTGTGG 
GTCACCGGCA GCCGCTTCTC CTACCTCATC AGCAAGGACA CGTGGCTGGA AGCGTGGCCC 
TTGGAGGAGT CGTGCCAGGA TGCCGACCTG CAGCCGCTCT GCCAGGACTT CACCGAGTTC 
TCCGACAATC TGGTCTTGTT TGGGTGCCCC ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990736.2
CDS	26..4981
Translation	MGLLLLPLLLGVLLLHAVPTPAQMVTMVTPAVLRLDTDEKVVLEAPGLSAPTEANILVQDFPQKRKVLFQVRKQLNPAEGMMAIATVKVPVKLLPPVVGKHFVSVVARVGQVTLEKVLLVSLQSGHIFLQTDKPIYTPGSTVLCRLFALSHFMQPLLKTVIVEVKTPDNVIIKQVPVSSPMRNGIFSINHNLPEVVSLGTWTITAKFEDSQDQVFSTQFEVKEYVLPSFEVTLDPQEKFLYIDRQEDFRVTITARYLYGKNLQGTAFVLFGVVVDDEKKTIPQSLQRVKVTDGDGEAVLPMAMLRQRFANLQELVGHSLYVTVTVLTESGSDMVEAQRSGIRIVTSPYTIHFTHTPKYFKPGMPFDLTVYVTNPDNSPAPRIPVKADNFQGLVSTQRDGTAKLVLNMPANKNSVPITVRTDQKDLPPERQASRQIVAEAYQSQGNSGNYLHLAVGASQVQPGDNLPINFHLKSNRDDVRKSVSYFTYLILSKGHIVHVGRQPREGDQSLVTMSLPVTANLIPSFRIVAYYHVKPGEIIADSVWVDVKDTCMGSLVVRGASEADNRVHEPRTPMRLHIEGDHKAHVGLVAVDKAVYVLNKNKLTQSKVWDTVENSDIGCTPGSGRNHVGVFADAGLSLTSNVNINTEQRSEVQCAKPAKRKRRSVRLIKHKGTKMAEYSDKNLRKCCEDGMKENLMGYSCEKRATYVLDGKACTEAFLSCCLYIKGIRDEERELQYELARSEVDDAFLSDEDITSRSLFPESWLWQVEELTEPPNEQGISMKTLPIYLKDSITTWEVLAVSISENKGLCVADPYEITVMKEFFIDLRLPYSAVRNEQVEVRAILYNYWTNKIKVRVELMYNPALCSASTTKTRYQQIFQLEPQSSHAVPFVIVPLQLGQHDVEVKAAVWNSFVSDGVKKKLRVVPEGMRLEKTVKIVELDPKTLGNNGVQEVKVKAANLSDIVPNTESETKVSIQGNPVSILVEKATDGTKLKHLIVTPSGCGEQNMIGMTPTVIAVHYLDSTMQWETFGINRRTEAIELIKKGYTQQLAYRKEDGSFAAFTTRPSSTWLTAYVAKVFAMAINMVDIKPEVVCGAIKWLILEKQQPDGLFQEDAPVIHKEMVGGYHGAEPSVSLTAFVLSALQESQKICKNYVKSLDGSIAKASDYLSRKYQSLTRPYTVALTSYALALTGKLNSEKVLMKFSKDGTHWAERNAHTYNIEGTSYALLALLQMEKAELTGPVVRWLAQQNYFGGGYGSTQATILVFQALAQYHVALPRQLELNLDVSVLLPRRANAITYRIENNNALVARSAETKLNEDFTVKAEGTGKGTMTVVTVYNAKVPEKENKCDNFDLRVSVEDVKAGRELEGVIRSVKITICTRFLDTVDATMSILDISMLTGFSPDVQDLKSLSEGVERYISKFEIDHALSNRSNLIIYLDKVSHQVEECIAFRAHQHFQVGLIQPASVIVYSYYKIDDRCTRFYHPDKAGGQLRKICHGEVCCAEENCFIRVKKDNPITVNERIDLACKPGVDYVYKVKVVATEETPSHDNYIMSILTVIKMGTDENPGGSNRTFVSHKQCRDALSLQKGQDYLVWGLASDLWVTGSRFSYLISKDTWLEAWPLEESCQDADLQPLCQDFTEFSDNLVLFGCPT

Target ORF information:

RefSeq Version	NM_205405.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus complement component 3 (C3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205405.2

ORF Insert Sequence:

ATGGGGCTGC TGCTGCTGCC CCTCCTGCTC GGCGTTCTGC TGCTCCATGC GGTCCCCACA 
CCTGCACAGA TGGTGACCAT GGTGACCCCG GCGGTGCTGC GGTTGGACAC GGACGAGAAG 
GTGGTGTTGG AGGCTCCGGG TCTGTCCGCC CCCACCGAGG CCAACATCCT GGTGCAGGAT 
TTCCCCCAAA AGCGCAAAGT CCTCTTCCAG GTCCGCAAGC AGCTCAACCC CGCAGAGGGG 
ATGATGGCCA TCGCCACCGT CAAGGTGCCG GTGAAGCTGC TGCCGCCGGT GGTGGGGAAG 
CACTTTGTCT CGGTGGTGGC GCGGGTGGGA CAGGTGACCC TGGAGAAGGT GCTGTTGGTG 
TCACTGCAGA GCGGCCACAT CTTCCTGCAG ACCGACAAAC CCATCTACAC CCCCGGCTCC 
ACCGTGCTCT GCCGTCTCTT CGCCCTCAGC CACTTCATGC AGCCTCTGCT GAAGACGGTG 
ATCGTGGAGG TCAAGACACC CGACAACGTC ATCATCAAGC AAGTGCCCGT GTCCTCCCCC 
ATGAGGAATG GGATCTTCTC CATCAACCAC AACCTGCCGG AGGTGGTCAG CCTGGGGACA 
TGGACTATCA CGGCCAAATT TGAAGACTCG CAGGACCAGG TCTTCAGCAC ACAATTTGAA 
GTCAAGGAAT ACGTGCTGCC GAGCTTTGAG GTCACCCTGG ACCCGCAGGA GAAGTTCCTC 
TACATTGACC GGCAGGAGGA TTTCCGGGTG ACCATCACAG CCAGGTACCT GTATGGGAAG 
AATCTGCAGG GGACCGCCTT CGTCCTCTTC GGTGTGGTGG TGGACGACGA GAAAAAGACC 
ATCCCCCAGT CCCTGCAGCG CGTCAAGGTG ACCGATGGGG ACGGGGAGGC CGTGCTGCCC 
ATGGCCATGC TGCGGCAGCG CTTCGCCAAC CTCCAGGAGC TGGTGGGACA CTCTCTCTAC 
GTCACCGTCA CCGTCCTCAC CGAGTCAGGC AGTGACATGG TGGAGGCACA GCGCAGCGGC 
ATCCGCATAG TGACGTCCCC ATACACCATC CACTTCACCC ACACCCCCAA GTACTTCAAG 
CCAGGGATGC CCTTCGATCT GACGGTTTAT GTCACCAACC CCGATAATTC CCCGGCTCCG 
CGCATCCCCG TCAAGGCCGA CAACTTCCAG GGCCTCGTCT CCACGCAGCG AGATGGCACA 
GCCAAGCTGG TCCTCAACAT GCCAGCCAAC AAGAACTCCG TCCCCATCAC TGTGAGGACA 
GACCAGAAGG ATCTGCCCCC GGAGCGCCAG GCCTCGCGGC AGATAGTGGC CGAGGCGTAT 
CAAAGCCAGG GGAACTCTGG CAACTACCTT CACCTGGCAG TGGGTGCCAG CCAGGTGCAG 
CCCGGGGACA ACCTCCCCAT CAACTTCCAT CTCAAGAGCA ACAGAGATGA CGTCCGCAAA 
TCCGTTTCCT ACTTCACCTA CCTGATCCTG AGCAAGGGGC ACATTGTCCA CGTGGGACGG 
CAGCCAAGGG AAGGTGACCA GAGCCTGGTC ACGATGTCGC TTCCCGTGAC GGCCAACCTC 
ATCCCTTCCT TCCGTATCGT GGCCTACTAC CACGTGAAGC CTGGCGAGAT CATTGCTGAC 
TCCGTCTGGG TCGATGTCAA GGACACCTGC ATGGGCAGTC TGGTGGTGAG GGGAGCGTCG 
GAGGCTGACA ATCGTGTGCA TGAGCCAAGG ACCCCCATGC GGCTGCACAT CGAGGGCGAC 
CACAAAGCCC ACGTGGGGCT GGTGGCTGTG GACAAGGCTG TCTATGTCCT CAACAAGAAC 
AAACTCACTC AGAGTAAGGT GTGGGACACA GTGGAGAACA GCGACATCGG CTGCACGCCG 
GGCAGTGGGA GGAACCACGT GGGGGTCTTC GCCGATGCCG GCCTCAGCCT GACTTCAAAC 
GTGAACATCA ACACGGAGCA GAGAAGTGAG GTCCAGTGTG CAAAGCCTGC AAAACGCAAG 
CGCCGCTCCG TGAGGCTCAT CAAGCACAAG GGCACCAAGA TGGCCGAGTA CAGCGACAAG 
AACCTGCGCA AGTGCTGTGA GGACGGCATG AAGGAAAACC TGATGGGCTA CAGCTGCGAG 
AAACGGGCCA CCTACGTCCT CGATGGCAAA GCCTGCACCG AAGCCTTCCT CAGCTGCTGC 
CTCTACATCA AGGGCATCCG CGATGAGGAG CGCGAGCTGC AATACGAGCT GGCTCGAAGT 
GAGGTGGATG ACGCCTTCCT GAGTGATGAA GACATCACCT CACGGAGCCT CTTTCCAGAG 
AGCTGGCTAT GGCAGGTGGA GGAGCTGACA GAACCACCCA ATGAACAGGG CATCTCCATG 
AAGACGCTGC CCATATACCT GAAAGACTCC ATCACCACCT GGGAGGTTCT GGCTGTCAGT 
ATCTCTGAGA ACAAGGGTCT GTGCGTGGCC GACCCCTATG AGATTACGGT GATGAAGGAG 
TTCTTCATTG ACCTGCGCCT GCCCTACTCG GCAGTGAGGA ACGAGCAGGT GGAGGTCCGC 
GCCATTCTCT ACAACTACTG GACGAACAAG ATCAAGGTGC GCGTGGAGCT GATGTACAAC 
CCAGCCCTGT GCAGCGCATC CACCACCAAG ACGCGCTACC AGCAGATCTT CCAACTGGAA 
CCTCAGTCGT CGCACGCCGT GCCCTTCGTC ATCGTGCCCC TGCAGCTGGG GCAGCATGAC 
GTCGAGGTGA AGGCAGCTGT CTGGAACAGC TTTGTGTCTG ATGGCGTCAA GAAGAAGCTC 
AGAGTGGTGC CTGAAGGGAT GAGGCTGGAG AAGACAGTGA AGATAGTGGA GCTTGACCCA 
AAGACGCTGG GAAATAACGG TGTGCAAGAA GTGAAGGTGA AAGCAGCAAA CCTCTCTGAC 
ATCGTCCCCA ACACTGAGTC GGAGACCAAA GTCAGCATTC AAGGCAACCC TGTGTCCATC 
CTGGTGGAGA AAGCCACCGA TGGGACCAAG CTGAAACACC TCATTGTGAC CCCCTCGGGC 
TGTGGGGAGC AGAACATGAT TGGGATGACG CCCACCGTCA TTGCTGTCCA CTACCTGGAC 
AGCACAATGC AGTGGGAGAC CTTCGGTATC AACCGCCGCA CTGAAGCCAT CGAACTGATT 
AAAAAGGGTT ACACCCAACA ACTTGCATAC CGCAAAGAAG ACGGCTCATT TGCTGCCTTC 
ACTACCCGCC CATCGAGCAC CTGGTTGACA GCCTACGTGG CCAAGGTCTT TGCCATGGCC 
ATCAACATGG TGGACATCAA GCCGGAGGTG GTTTGTGGAG CCATCAAATG GCTCATTCTG 
GAGAAGCAAC AGCCAGATGG GCTTTTCCAA GAAGACGCTC CTGTCATCCA CAAGGAGATG 
GTGGGAGGCT ACCACGGTGC TGAGCCCAGT GTGTCCCTGA CAGCCTTCGT CCTCTCCGCG 
CTGCAGGAAT CCCAGAAGAT CTGCAAGAAC TACGTGAAAA GCCTGGATGG GAGCATTGCC 
AAAGCCTCCG ATTACCTCTC CCGGAAATAC CAATCTTTGA CTCGACCCTA CACGGTGGCC 
CTGACCTCCT ACGCCCTGGC CCTAACGGGG AAACTCAACA GCGAGAAAGT CCTGATGAAG 
TTCTCCAAAG ATGGCACCCA CTGGGCGGAA CGCAACGCCC ACACCTACAA CATCGAGGGG 
ACGTCCTACG CTCTGCTGGC GCTGCTGCAG ATGGAGAAGG CCGAGCTGAC GGGGCCGGTG 
GTCCGCTGGT TGGCCCAGCA GAACTACTTC GGTGGTGGCT ACGGATCCAC CCAGGCCACC 
ATCCTGGTGT TCCAGGCTCT GGCTCAGTAC CACGTGGCGC TGCCGCGGCA GCTGGAGCTC 
AACCTGGACG TGTCGGTGCT GCTGCCGCGC CGCGCCAACG CCATCACCTA CCGCATCGAG 
AACAACAACG CGCTGGTGGC ACGCTCAGCT GAGACCAAGC TGAACGAGGA CTTCACTGTG 
AAAGCAGAGG GCACTGGCAA GGGGACAATG ACAGTGGTGA CCGTCTACAA CGCCAAAGTG 
CCCGAGAAGG AAAACAAGTG TGACAACTTC GACCTGCGGG TCAGCGTGGA GGACGTGAAG 
GCGGGCCGGG AGCTGGAAGG GGTCATCCGC TCTGTCAAGA TCACCATCTG CACCAGGTTC 
CTGGACACCG TGGATGCCAC CATGTCCATC CTTGATATCT CCATGCTCAC CGGCTTCTCC 
CCTGACGTCC AGGACCTGAA GAGTCTCTCG GAGGGAGTGG AGAGGTACAT TTCCAAATTT 
GAGATCGACC ACGCGCTGTC GAACCGCAGC AACCTCATCA TCTACCTGGA CAAGGTCTCC 
CACCAAGTGG AGGAGTGCAT CGCCTTCAGG GCCCACCAGC ACTTCCAGGT GGGACTGATC 
CAGCCCGCCT CCGTCATTGT CTACAGCTAC TACAAGATCG ATGACCGCTG CACCCGCTTC 
TACCACCCGG ACAAGGCTGG TGGGCAGCTG AGGAAGATCT GCCATGGGGA AGTGTGCTGC 
GCTGAAGAAA ACTGCTTCAT ACGGGTGAAG AAGGACAATC CCATCACAGT CAATGAGCGC 
ATCGACCTTG CCTGCAAGCC AGGGGTGGAC TATGTGTACA AAGTGAAGGT GGTGGCAACA 
GAGGAGACGC CATCCCACGA CAACTACATC ATGTCCATCC TCACCGTCAT CAAGATGGGC 
ACTGATGAGA ACCCAGGTGG GAGCAACCGG ACCTTCGTGA GCCATAAGCA GTGCCGGGAT 
GCATTGAGTC TCCAGAAGGG ACAGGACTAC CTGGTGTGGG GGCTGGCGTC CGACCTGTGG 
GTCACCGGCA GCCGCTTCTC CTACCTCATC AGCAAGGACA CGTGGCTGGA AGCGTGGCCC 
TTGGAGGAGT CGTGCCAGGA TGCCGACCTG CAGCCGCTCT GCCAGGACTT CACCGAGTTC 
TCCGACAATC TGGTCTTGTT TGGGTGCCCC ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Splenic gene expression profiling in White Leghorn layer inoculated with the Salmonella enterica serovar Enteritidis.
Animal genetics46(6)617-26(2015 Dec)
Wu G,Liu L,Qi Y,Sun Y,Yang N,Xu G,Zhou H,Li X

Complement anaphylatoxin C3a is a potent inducer of embryonic chick retina regeneration.
Nature communications42312(2013)
Haynes T,Luz-Madrigal A,Reis ES,Echeverri Ruiz NP,Grajales-Esquivel E,Tzekou A,Tsonis PA,Lambris JD,Del Rio-Tsonis K

Cloning of a gene fragment encoding chicken complement component C3d with expression and immunogenicity of Newcastle disease virus F gene-C3d fusion protein.
Avian pathology : journal of the W.V.P.A37(5)477-85(2008 Oct)
Liu D,Niu ZX

Anti-ischemia/reperfusion of C1 inhibitor in myocardial cell injury via regulation of local myocardial C3 activity.
Biochemical and biophysical research communications350(1)162-8(2006 Nov)
Fu J,Lin G,Zeng B,Wu Z,Wu Y,Chu H,Qin G,Liang G,Li J,Gan X,Yu X,Li C,Liu D

Receptor-mediated transport and deposition of complement component C3 into developing chicken oocytes.
Cellular and molecular life sciences : CMLS62(16)1871-80(2005 Aug)
Recheis B,Rumpler H,Schneider WJ,Nimpf J

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C3 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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