C3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	C3
Entrez Gene ID	12266
Full Name	complement component 3
Synonyms	AI255234,ASP,HSE-MSF,Plp
General protein information	Preferred Names complement component 3 Names complement C3 acylation stimulating protein complement component 3d complement factor 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 D\|17 29.72 cM
Summary	This gene encodes complement protein C3 which plays a central role in the classical, alternative and lectin activation pathways of the complement system. The encoded preproprotein undergoes a multi-step processing to generate various functional peptides. Mice deficient in the encoded protein fail to clear bacteria from the blood stream upon infection, display diminished airway hyperresponsiveness and lung eosinophilia upon allergen-induced pulmonary allergy, and develop severe lung injury after deposition of IgG immune complexes. Deficiency of the homolog of the encoded protein in humans was found to be associated with increased susceptibility to infections, age-related macular degeneration, and atypical hemolytic uremic syndrome. [provided by RefSeq, Mar 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_011246258.1	XP_011244560.1	complement C3 isoform X1
XM_011246258.2	XP_011244560.1	complement C3 isoform X1
NM_009778.3	NP_033908.2	complement C3 preproprotein

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	c3c	XP_005168460.1
Danio rerio (zebrafish)	LOC100331492	XP_002660624.3
Danio rerio (zebrafish)	c3a	NP_571317.1
Xenopus tropicalis (tropical clawed frog)	c3	XP_004919147.1
Xenopus tropicalis (tropical clawed frog)	LOC100492293	XP_002940095.2
Xenopus tropicalis (tropical clawed frog)	LOC100497914	XP_002941061.2
Canis lupus familiaris (dog)	C3	XP_005633285.1
Bos taurus (cattle)	C3	NP_001035559.2
Mus musculus (house mouse)	C3	NP_033908.2
Rattus norvegicus (Norway rat)	C3	NP_058690.2
Xenopus tropicalis (tropical clawed frog)	LOC100492966	XP_002940099.2
Homo sapiens (human)	C3	NP_000055.2
Gallus gallus (chicken)	C3	NP_990736.1
Danio rerio (zebrafish)	LOC100331428	XP_002660623.3
Xenopus tropicalis (tropical clawed frog)	LOC100491968	XP_004919148.1
Pan troglodytes (chimpanzee)	C3	XP_512318.4
	C3	XP_001091921.2
Danio rerio (zebrafish)	zgc:103710	NP_001008582.3
Danio rerio (zebrafish)	c3b	NP_571318.1
Xenopus tropicalis (tropical clawed frog)	LOC100492134	XP_002940094.2
Xenopus tropicalis (tropical clawed frog)	LOC100497762	XP_004919229.1

Gene Ontology
Bibliography

Related articles in PubMed

Acylation-stimulating protein deficiency and altered adipose tissue in alternative complement pathway knockout mice.
Paglialunga S, Fisette A, Yan Y, Deshaies Y, Brouillette JF, Pekna M, Cianflone K
American journal of physiology. Endocrinology and metabolism294(3)E521-9(2008 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Retinal C3 was expressed by microglia/macrophages located in the outer retina in AMD eyes. In rodent photo-oxidative damage, C3-expressing microglia/macrophages and complement activation were located in regions of lesion expansion in the outer retina over 2 months
Title: Retinal Macrophages Synthesize C3 and Activate Complement in AMD and in Models of Focal Retinal Degeneration.

Study found that cancer-cell-derived C3 activates the C3a receptor in the choroid plexus epithelium to disrupt the blood-cerebrospinal fluid (CSF) barrier. This effect allows plasma components, including amphiregulin, and other mitogens to enter the CSF and promote cancer cell growth.
Title: Complement Component 3 Adapts the Cerebrospinal Fluid for Leptomeningeal Metastasis.

Demonstrate local synthesis of complement proteins by both PDGFRbeta-positive pericytes and CD45-positive cells in kidney fibrosis.
Title: Pericytes and immune cells contribute to complement activation in tubulointerstitial fibrosis.

nutrient sensing in the liver is coupled to release of C3 and potentially its metabolic and inflammatory functions.
Title: Liver ubiquitome uncovers nutrient-stress-mediated trafficking and secretion of complement C3.

These data indicated that alpha7-nAChR caused the inhibition of ASPinduced activation of p38 kinase and NFkappa B to inhibit the production of MCP1 and keratinocytederived chemokine.
Title: Nicotinic ?7 receptor inhibits the acylation stimulating protein?induced production of monocyte chemoattractant protein?1 and keratinocyte?derived chemokine in adipocytes by modulating the p38 kinase and nuclear factor??B signaling pathways.

The following C3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the C3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu67230	XM_011246258.2 Latest version!	Mus musculus complement component 3 (C3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu22069	NM_009778.3 Latest version!	Mus musculus complement component 3 (C3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$979.30 $1399.00
View Old Accession Versions >>
OMu67230	XM_011246258.1	Mus musculus complement component 3 (C3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

C3 ( XM_011246258.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244560.1 Mus musculus complement component 3 (C3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu67230
Clone ID Related Accession (Same CDS sequence)	XM_011246258.1 , XM_011246258.2
Accession Version	XM_011246258.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	complement C3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAAC ACAACTGCCC AGTGCTGGGC CTGGCCAGGA GTGAATTGGA GGAAGACATA 
ATTCCAGAAG AAGATATTAT CTCTAGAAGC CACTTCCCAC AGAGCTGGTT GTGGACCATA 
GAAGAGTTGA AAGAACCAGA GAAAAATGGA ATCTCTACGA AGGTCATGAA CATCTTTCTC 
AAAGATTCCA TCACCACCTG GGAGATTCTG GCAGTGAGCT TGTCAGACAA GAAAGGGATC 
TGTGTGGCAG ACCCCTATGA GATCAGAGTG ATGCAGGACT TCTTCATTGA CCTGCGGCTG 
CCCTACTCTG TAGTGCGCAA CGAACAGGTG GAGATCAGAG CTGTGCTCTT CAACTACCGT 
GAACAGGAGG AACTTAAGGT GAGGGTGGAA CTGTTGCATA ATCCAGCCTT CTGCAGCATG 
GCCACCGCCA AGAATCGCTA CTTCCAGACC ATCAAAATCC CTCCCAAGTC CTCGGTGGCT 
GTACCGTATG TCATTGTCCC CTTGAAGATC GGCCAACAAG AGGTGGAGGT CAAGGCTGCT 
GTCTTCAATC ACTTCATCAG TGATGGTGTC AAGAAGACAC TGAAGGTCGT GCCAGAAGGA 
ATGAGAATCA ACAAAACTGT GGCCATCCAT ACACTGGACC CAGAGAAGCT CGGTCAAGGG 
GGAGTGCAGA AGGTGGATGT GCCTGCCGCA GACCTTAGCG ACCAAGTGCC AGACACAGAC 
TCTGAGACCA GAATTATCCT GCAAGGGAGC CCGGTGGTTC AGATGGCTGA AGATGCTGTG 
GACGGGGAGC GGCTGAAACA CCTGATCGTG ACCCCCGCAG GCTGTGGGGA ACAGAACATG 
ATTGGCATGA CACCAACAGT CATTGCGGTA CACTACCTGG ACCAGACCGA ACAGTGGGAG 
AAGTTCGGCA TAGAGAAGAG GCAAGAGGCC CTGGAGCTCA TCAAGAAAGG GTACACCCAG 
CAGCTGGCCT TCAAACAGCC CAGCTCTGCC TATGCTGCCT TCAACAACCG GCCCCCCAGC 
ACCTGGCTGA CAGCCTACGT GGTCAAGGTC TTCTCTCTAG CTGCCAACCT CATCGCCATC 
GACTCTCACG TCCTGTGTGG GGCTGTTAAA TGGTTGATTC TGGAGAAACA GAAGCCGGAT 
GGTGTCTTTC AGGAGGATGG GCCCGTGATT CACCAAGAAA TGATTGGTGG CTTCCGGAAC 
GCCAAGGAGG CAGATGTGTC ACTCACAGCC TTCGTCCTCA TCGCACTGCA GGAAGCCAGG 
GACATCTGTG AGGGGCAGGT CAATAGCCTT CCTGGGAGCA TCAACAAGGC AGGGGAGTAT 
ATTGAAGCCA GTTACATGAA CCTGCAGAGA CCATACACAG TGGCCATTGC TGGGTATGCC 
CTGGCCCTGA TGAACAAACT GGAGGAACCT TACCTCGGCA AGTTTCTGAA CACAGCCAAA 
GATCGGAACC GCTGGGAGGA GCCTGACCAG CAGCTCTACA ACGTAGAGGC CACATCCTAC 
GCCCTCCTGG CCCTGCTGCT GCTGAAAGAC TTTGACTCTG TGCCCCCTGT AGTGCGCTGG 
CTCAATGAGC AAAGATACTA CGGAGGCGGC TATGGCTCCA CCCAGGCTAC CTTCATGGTA 
TTCCAAGCCT TGGCCCAATA TCAAACAGAT GTCCCTGACC ATAAGGACTT GAACATGGAT 
GTGTCCTTCC ACCTCCCCAG CCGTAGCTCT GCAACCACGT TTCGCCTGCT CTGGGAAAAT 
GGCAACCTCC TGCGATCGGA AGAGACCAAG CAAAATGAGG CCTTCTCTCT AACAGCCAAA 
GGAAAAGGCC GAGGCACATT GTCGGTGGTG GCAGTGTATC ATGCCAAACT CAAAAGCAAA 
GTCACCTGCA AGAAGTTTGA CCTCAGGGTC AGCATAAGAC CAGCCCCTGA GACAGCCAAG 
AAGCCCGAGG AAGCCAAGAA TACCATGTTC CTTGAAATCT GCACCAAGTA CTTGGGAGAT 
GTGGACGCCA CTATGTCCAT CCTGGACATC TCCATGATGA CTGGCTTTGC TCCAGACACA 
AAGGACCTGG AACTGCTGGC CTCTGGAGTA GATAGATACA TCTCCAAGTA CGAGATGAAC 
AAAGCCTTCT CCAACAAGAA CACCCTCATC ATCTACCTAG AAAAGATTTC ACACACCGAA 
GAAGACTGCC TGACCTTCAA AGTTCACCAG TACTTTAATG TGGGACTTAT CCAGCCCGGG 
TCGGTCAAGG TCTACTCCTA TTACAACCTC GAGGAATCAT GCACCCGGTT CTATCATCCA 
GAGAAGGACG ATGGGATGCT CAGCAAGCTG TGCCACAGTG AAATGTGCCG GTGTGCTGAA 
GAGAACTGCT TCATGCAACA GTCACAGGAG AAGATCAACC TGAATGTCCG GCTAGACAAG 
GCTTGTGAGC CCGGAGTCGA CTATGTGTAC AAGACCGAGC TAACCAACAT AGAGCTGTTG 
GATGATTTTG ATGAGTACAC CATGACCATC CAGCAGGTCA TCAAGTCAGG CTCAGATGAG 
GTGCAGGCAG GGCAGCAACG CAAGTTCATC AGCCACATCA AGTGCAGAAA CGCCCTGAAG 
CTGCAGAAAG GGAAGAAGTA CCTCATGTGG GGCCTCTCCT CTGACCTCTG GGGAGAAAAG 
CCCAACACCA GCTACATCAT TGGGAAGGAC ACGTGGGTGG AGCACTGGCC TGAGGCAGAA 
GAATGCCAGG ATCAGAAGTA CCAGAAACAG TGCGAAGAAC TTGGGGCATT CACAGAATCT 
ATGGTGGTTT ATGGTTGTCC CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244560.1
CDS	30..2816
Translation	MGEHNCPVLGLARSELEEDIIPEEDIISRSHFPQSWLWTIEELKEPEKNGISTKVMNIFLKDSITTWEILAVSLSDKKGICVADPYEIRVMQDFFIDLRLPYSVVRNEQVEIRAVLFNYREQEELKVRVELLHNPAFCSMATAKNRYFQTIKIPPKSSVAVPYVIVPLKIGQQEVEVKAAVFNHFISDGVKKTLKVVPEGMRINKTVAIHTLDPEKLGQGGVQKVDVPAADLSDQVPDTDSETRIILQGSPVVQMAEDAVDGERLKHLIVTPAGCGEQNMIGMTPTVIAVHYLDQTEQWEKFGIEKRQEALELIKKGYTQQLAFKQPSSAYAAFNNRPPSTWLTAYVVKVFSLAANLIAIDSHVLCGAVKWLILEKQKPDGVFQEDGPVIHQEMIGGFRNAKEADVSLTAFVLIALQEARDICEGQVNSLPGSINKAGEYIEASYMNLQRPYTVAIAGYALALMNKLEEPYLGKFLNTAKDRNRWEEPDQQLYNVEATSYALLALLLLKDFDSVPPVVRWLNEQRYYGGGYGSTQATFMVFQALAQYQTDVPDHKDLNMDVSFHLPSRSSATTFRLLWENGNLLRSEETKQNEAFSLTAKGKGRGTLSVVAVYHAKLKSKVTCKKFDLRVSIRPAPETAKKPEEAKNTMFLEICTKYLGDVDATMSILDISMMTGFAPDTKDLELLASGVDRYISKYEMNKAFSNKNTLIIYLEKISHTEEDCLTFKVHQYFNVGLIQPGSVKVYSYYNLEESCTRFYHPEKDDGMLSKLCHSEMCRCAEENCFMQQSQEKINLNVRLDKACEPGVDYVYKTELTNIELLDDFDEYTMTIQQVIKSGSDEVQAGQQRKFISHIKCRNALKLQKGKKYLMWGLSSDLWGEKPNTSYIIGKDTWVEHWPEAEECQDQKYQKQCEELGAFTESMVVYGCPN

Target ORF information:

RefSeq Version	XM_011246258.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus complement component 3 (C3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246258.1

ORF Insert Sequence:

ATGGGGGAAC ACAACTGCCC AGTGCTGGGC CTGGCCAGGA GTGAATTGGA GGAAGACATA 
ATTCCAGAAG AAGATATTAT CTCTAGAAGC CACTTCCCAC AGAGCTGGTT GTGGACCATA 
GAAGAGTTGA AAGAACCAGA GAAAAATGGA ATCTCTACGA AGGTCATGAA CATCTTTCTC 
AAAGATTCCA TCACCACCTG GGAGATTCTG GCAGTGAGCT TGTCAGACAA GAAAGGGATC 
TGTGTGGCAG ACCCCTATGA GATCAGAGTG ATGCAGGACT TCTTCATTGA CCTGCGGCTG 
CCCTACTCTG TAGTGCGCAA CGAACAGGTG GAGATCAGAG CTGTGCTCTT CAACTACCGT 
GAACAGGAGG AACTTAAGGT GAGGGTGGAA CTGTTGCATA ATCCAGCCTT CTGCAGCATG 
GCCACCGCCA AGAATCGCTA CTTCCAGACC ATCAAAATCC CTCCCAAGTC CTCGGTGGCT 
GTACCGTATG TCATTGTCCC CTTGAAGATC GGCCAACAAG AGGTGGAGGT CAAGGCTGCT 
GTCTTCAATC ACTTCATCAG TGATGGTGTC AAGAAGACAC TGAAGGTCGT GCCAGAAGGA 
ATGAGAATCA ACAAAACTGT GGCCATCCAT ACACTGGACC CAGAGAAGCT CGGTCAAGGG 
GGAGTGCAGA AGGTGGATGT GCCTGCCGCA GACCTTAGCG ACCAAGTGCC AGACACAGAC 
TCTGAGACCA GAATTATCCT GCAAGGGAGC CCGGTGGTTC AGATGGCTGA AGATGCTGTG 
GACGGGGAGC GGCTGAAACA CCTGATCGTG ACCCCCGCAG GCTGTGGGGA ACAGAACATG 
ATTGGCATGA CACCAACAGT CATTGCGGTA CACTACCTGG ACCAGACCGA ACAGTGGGAG 
AAGTTCGGCA TAGAGAAGAG GCAAGAGGCC CTGGAGCTCA TCAAGAAAGG GTACACCCAG 
CAGCTGGCCT TCAAACAGCC CAGCTCTGCC TATGCTGCCT TCAACAACCG GCCCCCCAGC 
ACCTGGCTGA CAGCCTACGT GGTCAAGGTC TTCTCTCTAG CTGCCAACCT CATCGCCATC 
GACTCTCACG TCCTGTGTGG GGCTGTTAAA TGGTTGATTC TGGAGAAACA GAAGCCGGAT 
GGTGTCTTTC AGGAGGATGG GCCCGTGATT CACCAAGAAA TGATTGGTGG CTTCCGGAAC 
GCCAAGGAGG CAGATGTGTC ACTCACAGCC TTCGTCCTCA TCGCACTGCA GGAAGCCAGG 
GACATCTGTG AGGGGCAGGT CAATAGCCTT CCTGGGAGCA TCAACAAGGC AGGGGAGTAT 
ATTGAAGCCA GTTACATGAA CCTGCAGAGA CCATACACAG TGGCCATTGC TGGGTATGCC 
CTGGCCCTGA TGAACAAACT GGAGGAACCT TACCTCGGCA AGTTTCTGAA CACAGCCAAA 
GATCGGAACC GCTGGGAGGA GCCTGACCAG CAGCTCTACA ACGTAGAGGC CACATCCTAC 
GCCCTCCTGG CCCTGCTGCT GCTGAAAGAC TTTGACTCTG TGCCCCCTGT AGTGCGCTGG 
CTCAATGAGC AAAGATACTA CGGAGGCGGC TATGGCTCCA CCCAGGCTAC CTTCATGGTA 
TTCCAAGCCT TGGCCCAATA TCAAACAGAT GTCCCTGACC ATAAGGACTT GAACATGGAT 
GTGTCCTTCC ACCTCCCCAG CCGTAGCTCT GCAACCACGT TTCGCCTGCT CTGGGAAAAT 
GGCAACCTCC TGCGATCGGA AGAGACCAAG CAAAATGAGG CCTTCTCTCT AACAGCCAAA 
GGAAAAGGCC GAGGCACATT GTCGGTGGTG GCAGTGTATC ATGCCAAACT CAAAAGCAAA 
GTCACCTGCA AGAAGTTTGA CCTCAGGGTC AGCATAAGAC CAGCCCCTGA GACAGCCAAG 
AAGCCCGAGG AAGCCAAGAA TACCATGTTC CTTGAAATCT GCACCAAGTA CTTGGGAGAT 
GTGGACGCCA CTATGTCCAT CCTGGACATC TCCATGATGA CTGGCTTTGC TCCAGACACA 
AAGGACCTGG AACTGCTGGC CTCTGGAGTA GATAGATACA TCTCCAAGTA CGAGATGAAC 
AAAGCCTTCT CCAACAAGAA CACCCTCATC ATCTACCTAG AAAAGATTTC ACACACCGAA 
GAAGACTGCC TGACCTTCAA AGTTCACCAG TACTTTAATG TGGGACTTAT CCAGCCCGGG 
TCGGTCAAGG TCTACTCCTA TTACAACCTC GAGGAATCAT GCACCCGGTT CTATCATCCA 
GAGAAGGACG ATGGGATGCT CAGCAAGCTG TGCCACAGTG AAATGTGCCG GTGTGCTGAA 
GAGAACTGCT TCATGCAACA GTCACAGGAG AAGATCAACC TGAATGTCCG GCTAGACAAG 
GCTTGTGAGC CCGGAGTCGA CTATGTGTAC AAGACCGAGC TAACCAACAT AGAGCTGTTG 
GATGATTTTG ATGAGTACAC CATGACCATC CAGCAGGTCA TCAAGTCAGG CTCAGATGAG 
GTGCAGGCAG GGCAGCAACG CAAGTTCATC AGCCACATCA AGTGCAGAAA CGCCCTGAAG 
CTGCAGAAAG GGAAGAAGTA CCTCATGTGG GGCCTCTCCT CTGACCTCTG GGGAGAAAAG 
CCCAACACCA GCTACATCAT TGGGAAGGAC ACGTGGGTGG AGCACTGGCC TGAGGCAGAA 
GAATGCCAGG ATCAGAAGTA CCAGAAACAG TGCGAAGAAC TTGGGGCATT CACAGAATCT 
ATGGTGGTTT ATGGTTGTCC CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C3 ( XM_011246258.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244560.1 Mus musculus complement component 3 (C3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu67230
Clone ID Related Accession (Same CDS sequence)	XM_011246258.1 , XM_011246258.2
Accession Version	XM_011246258.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	complement C3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246258.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAAC ACAACTGCCC AGTGCTGGGC CTGGCCAGGA GTGAATTGGA GGAAGACATA 
ATTCCAGAAG AAGATATTAT CTCTAGAAGC CACTTCCCAC AGAGCTGGTT GTGGACCATA 
GAAGAGTTGA AAGAACCAGA GAAAAATGGA ATCTCTACGA AGGTCATGAA CATCTTTCTC 
AAAGATTCCA TCACCACCTG GGAGATTCTG GCAGTGAGCT TGTCAGACAA GAAAGGGATC 
TGTGTGGCAG ACCCCTATGA GATCAGAGTG ATGCAGGACT TCTTCATTGA CCTGCGGCTG 
CCCTACTCTG TAGTGCGCAA CGAACAGGTG GAGATCAGAG CTGTGCTCTT CAACTACCGT 
GAACAGGAGG AACTTAAGGT GAGGGTGGAA CTGTTGCATA ATCCAGCCTT CTGCAGCATG 
GCCACCGCCA AGAATCGCTA CTTCCAGACC ATCAAAATCC CTCCCAAGTC CTCGGTGGCT 
GTACCGTATG TCATTGTCCC CTTGAAGATC GGCCAACAAG AGGTGGAGGT CAAGGCTGCT 
GTCTTCAATC ACTTCATCAG TGATGGTGTC AAGAAGACAC TGAAGGTCGT GCCAGAAGGA 
ATGAGAATCA ACAAAACTGT GGCCATCCAT ACACTGGACC CAGAGAAGCT CGGTCAAGGG 
GGAGTGCAGA AGGTGGATGT GCCTGCCGCA GACCTTAGCG ACCAAGTGCC AGACACAGAC 
TCTGAGACCA GAATTATCCT GCAAGGGAGC CCGGTGGTTC AGATGGCTGA AGATGCTGTG 
GACGGGGAGC GGCTGAAACA CCTGATCGTG ACCCCCGCAG GCTGTGGGGA ACAGAACATG 
ATTGGCATGA CACCAACAGT CATTGCGGTA CACTACCTGG ACCAGACCGA ACAGTGGGAG 
AAGTTCGGCA TAGAGAAGAG GCAAGAGGCC CTGGAGCTCA TCAAGAAAGG GTACACCCAG 
CAGCTGGCCT TCAAACAGCC CAGCTCTGCC TATGCTGCCT TCAACAACCG GCCCCCCAGC 
ACCTGGCTGA CAGCCTACGT GGTCAAGGTC TTCTCTCTAG CTGCCAACCT CATCGCCATC 
GACTCTCACG TCCTGTGTGG GGCTGTTAAA TGGTTGATTC TGGAGAAACA GAAGCCGGAT 
GGTGTCTTTC AGGAGGATGG GCCCGTGATT CACCAAGAAA TGATTGGTGG CTTCCGGAAC 
GCCAAGGAGG CAGATGTGTC ACTCACAGCC TTCGTCCTCA TCGCACTGCA GGAAGCCAGG 
GACATCTGTG AGGGGCAGGT CAATAGCCTT CCTGGGAGCA TCAACAAGGC AGGGGAGTAT 
ATTGAAGCCA GTTACATGAA CCTGCAGAGA CCATACACAG TGGCCATTGC TGGGTATGCC 
CTGGCCCTGA TGAACAAACT GGAGGAACCT TACCTCGGCA AGTTTCTGAA CACAGCCAAA 
GATCGGAACC GCTGGGAGGA GCCTGACCAG CAGCTCTACA ACGTAGAGGC CACATCCTAC 
GCCCTCCTGG CCCTGCTGCT GCTGAAAGAC TTTGACTCTG TGCCCCCTGT AGTGCGCTGG 
CTCAATGAGC AAAGATACTA CGGAGGCGGC TATGGCTCCA CCCAGGCTAC CTTCATGGTA 
TTCCAAGCCT TGGCCCAATA TCAAACAGAT GTCCCTGACC ATAAGGACTT GAACATGGAT 
GTGTCCTTCC ACCTCCCCAG CCGTAGCTCT GCAACCACGT TTCGCCTGCT CTGGGAAAAT 
GGCAACCTCC TGCGATCGGA AGAGACCAAG CAAAATGAGG CCTTCTCTCT AACAGCCAAA 
GGAAAAGGCC GAGGCACATT GTCGGTGGTG GCAGTGTATC ATGCCAAACT CAAAAGCAAA 
GTCACCTGCA AGAAGTTTGA CCTCAGGGTC AGCATAAGAC CAGCCCCTGA GACAGCCAAG 
AAGCCCGAGG AAGCCAAGAA TACCATGTTC CTTGAAATCT GCACCAAGTA CTTGGGAGAT 
GTGGACGCCA CTATGTCCAT CCTGGACATC TCCATGATGA CTGGCTTTGC TCCAGACACA 
AAGGACCTGG AACTGCTGGC CTCTGGAGTA GATAGATACA TCTCCAAGTA CGAGATGAAC 
AAAGCCTTCT CCAACAAGAA CACCCTCATC ATCTACCTAG AAAAGATTTC ACACACCGAA 
GAAGACTGCC TGACCTTCAA AGTTCACCAG TACTTTAATG TGGGACTTAT CCAGCCCGGG 
TCGGTCAAGG TCTACTCCTA TTACAACCTC GAGGAATCAT GCACCCGGTT CTATCATCCA 
GAGAAGGACG ATGGGATGCT CAGCAAGCTG TGCCACAGTG AAATGTGCCG GTGTGCTGAA 
GAGAACTGCT TCATGCAACA GTCACAGGAG AAGATCAACC TGAATGTCCG GCTAGACAAG 
GCTTGTGAGC CCGGAGTCGA CTATGTGTAC AAGACCGAGC TAACCAACAT AGAGCTGTTG 
GATGATTTTG ATGAGTACAC CATGACCATC CAGCAGGTCA TCAAGTCAGG CTCAGATGAG 
GTGCAGGCAG GGCAGCAACG CAAGTTCATC AGCCACATCA AGTGCAGAAA CGCCCTGAAG 
CTGCAGAAAG GGAAGAAGTA CCTCATGTGG GGCCTCTCCT CTGACCTCTG GGGAGAAAAG 
CCCAACACCA GCTACATCAT TGGGAAGGAC ACGTGGGTGG AGCACTGGCC TGAGGCAGAA 
GAATGCCAGG ATCAGAAGTA CCAGAAACAG TGCGAAGAAC TTGGGGCATT CACAGAATCT 
ATGGTGGTTT ATGGTTGTCC CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244560.1
CDS	30..2816
Translation	MGEHNCPVLGLARSELEEDIIPEEDIISRSHFPQSWLWTIEELKEPEKNGISTKVMNIFLKDSITTWEILAVSLSDKKGICVADPYEIRVMQDFFIDLRLPYSVVRNEQVEIRAVLFNYREQEELKVRVELLHNPAFCSMATAKNRYFQTIKIPPKSSVAVPYVIVPLKIGQQEVEVKAAVFNHFISDGVKKTLKVVPEGMRINKTVAIHTLDPEKLGQGGVQKVDVPAADLSDQVPDTDSETRIILQGSPVVQMAEDAVDGERLKHLIVTPAGCGEQNMIGMTPTVIAVHYLDQTEQWEKFGIEKRQEALELIKKGYTQQLAFKQPSSAYAAFNNRPPSTWLTAYVVKVFSLAANLIAIDSHVLCGAVKWLILEKQKPDGVFQEDGPVIHQEMIGGFRNAKEADVSLTAFVLIALQEARDICEGQVNSLPGSINKAGEYIEASYMNLQRPYTVAIAGYALALMNKLEEPYLGKFLNTAKDRNRWEEPDQQLYNVEATSYALLALLLLKDFDSVPPVVRWLNEQRYYGGGYGSTQATFMVFQALAQYQTDVPDHKDLNMDVSFHLPSRSSATTFRLLWENGNLLRSEETKQNEAFSLTAKGKGRGTLSVVAVYHAKLKSKVTCKKFDLRVSIRPAPETAKKPEEAKNTMFLEICTKYLGDVDATMSILDISMMTGFAPDTKDLELLASGVDRYISKYEMNKAFSNKNTLIIYLEKISHTEEDCLTFKVHQYFNVGLIQPGSVKVYSYYNLEESCTRFYHPEKDDGMLSKLCHSEMCRCAEENCFMQQSQEKINLNVRLDKACEPGVDYVYKTELTNIELLDDFDEYTMTIQQVIKSGSDEVQAGQQRKFISHIKCRNALKLQKGKKYLMWGLSSDLWGEKPNTSYIIGKDTWVEHWPEAEECQDQKYQKQCEELGAFTESMVVYGCPN

Target ORF information:

RefSeq Version	XM_011246258.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus complement component 3 (C3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246258.2

ORF Insert Sequence:

ATGGGGGAAC ACAACTGCCC AGTGCTGGGC CTGGCCAGGA GTGAATTGGA GGAAGACATA 
ATTCCAGAAG AAGATATTAT CTCTAGAAGC CACTTCCCAC AGAGCTGGTT GTGGACCATA 
GAAGAGTTGA AAGAACCAGA GAAAAATGGA ATCTCTACGA AGGTCATGAA CATCTTTCTC 
AAAGATTCCA TCACCACCTG GGAGATTCTG GCAGTGAGCT TGTCAGACAA GAAAGGGATC 
TGTGTGGCAG ACCCCTATGA GATCAGAGTG ATGCAGGACT TCTTCATTGA CCTGCGGCTG 
CCCTACTCTG TAGTGCGCAA CGAACAGGTG GAGATCAGAG CTGTGCTCTT CAACTACCGT 
GAACAGGAGG AACTTAAGGT GAGGGTGGAA CTGTTGCATA ATCCAGCCTT CTGCAGCATG 
GCCACCGCCA AGAATCGCTA CTTCCAGACC ATCAAAATCC CTCCCAAGTC CTCGGTGGCT 
GTACCGTATG TCATTGTCCC CTTGAAGATC GGCCAACAAG AGGTGGAGGT CAAGGCTGCT 
GTCTTCAATC ACTTCATCAG TGATGGTGTC AAGAAGACAC TGAAGGTCGT GCCAGAAGGA 
ATGAGAATCA ACAAAACTGT GGCCATCCAT ACACTGGACC CAGAGAAGCT CGGTCAAGGG 
GGAGTGCAGA AGGTGGATGT GCCTGCCGCA GACCTTAGCG ACCAAGTGCC AGACACAGAC 
TCTGAGACCA GAATTATCCT GCAAGGGAGC CCGGTGGTTC AGATGGCTGA AGATGCTGTG 
GACGGGGAGC GGCTGAAACA CCTGATCGTG ACCCCCGCAG GCTGTGGGGA ACAGAACATG 
ATTGGCATGA CACCAACAGT CATTGCGGTA CACTACCTGG ACCAGACCGA ACAGTGGGAG 
AAGTTCGGCA TAGAGAAGAG GCAAGAGGCC CTGGAGCTCA TCAAGAAAGG GTACACCCAG 
CAGCTGGCCT TCAAACAGCC CAGCTCTGCC TATGCTGCCT TCAACAACCG GCCCCCCAGC 
ACCTGGCTGA CAGCCTACGT GGTCAAGGTC TTCTCTCTAG CTGCCAACCT CATCGCCATC 
GACTCTCACG TCCTGTGTGG GGCTGTTAAA TGGTTGATTC TGGAGAAACA GAAGCCGGAT 
GGTGTCTTTC AGGAGGATGG GCCCGTGATT CACCAAGAAA TGATTGGTGG CTTCCGGAAC 
GCCAAGGAGG CAGATGTGTC ACTCACAGCC TTCGTCCTCA TCGCACTGCA GGAAGCCAGG 
GACATCTGTG AGGGGCAGGT CAATAGCCTT CCTGGGAGCA TCAACAAGGC AGGGGAGTAT 
ATTGAAGCCA GTTACATGAA CCTGCAGAGA CCATACACAG TGGCCATTGC TGGGTATGCC 
CTGGCCCTGA TGAACAAACT GGAGGAACCT TACCTCGGCA AGTTTCTGAA CACAGCCAAA 
GATCGGAACC GCTGGGAGGA GCCTGACCAG CAGCTCTACA ACGTAGAGGC CACATCCTAC 
GCCCTCCTGG CCCTGCTGCT GCTGAAAGAC TTTGACTCTG TGCCCCCTGT AGTGCGCTGG 
CTCAATGAGC AAAGATACTA CGGAGGCGGC TATGGCTCCA CCCAGGCTAC CTTCATGGTA 
TTCCAAGCCT TGGCCCAATA TCAAACAGAT GTCCCTGACC ATAAGGACTT GAACATGGAT 
GTGTCCTTCC ACCTCCCCAG CCGTAGCTCT GCAACCACGT TTCGCCTGCT CTGGGAAAAT 
GGCAACCTCC TGCGATCGGA AGAGACCAAG CAAAATGAGG CCTTCTCTCT AACAGCCAAA 
GGAAAAGGCC GAGGCACATT GTCGGTGGTG GCAGTGTATC ATGCCAAACT CAAAAGCAAA 
GTCACCTGCA AGAAGTTTGA CCTCAGGGTC AGCATAAGAC CAGCCCCTGA GACAGCCAAG 
AAGCCCGAGG AAGCCAAGAA TACCATGTTC CTTGAAATCT GCACCAAGTA CTTGGGAGAT 
GTGGACGCCA CTATGTCCAT CCTGGACATC TCCATGATGA CTGGCTTTGC TCCAGACACA 
AAGGACCTGG AACTGCTGGC CTCTGGAGTA GATAGATACA TCTCCAAGTA CGAGATGAAC 
AAAGCCTTCT CCAACAAGAA CACCCTCATC ATCTACCTAG AAAAGATTTC ACACACCGAA 
GAAGACTGCC TGACCTTCAA AGTTCACCAG TACTTTAATG TGGGACTTAT CCAGCCCGGG 
TCGGTCAAGG TCTACTCCTA TTACAACCTC GAGGAATCAT GCACCCGGTT CTATCATCCA 
GAGAAGGACG ATGGGATGCT CAGCAAGCTG TGCCACAGTG AAATGTGCCG GTGTGCTGAA 
GAGAACTGCT TCATGCAACA GTCACAGGAG AAGATCAACC TGAATGTCCG GCTAGACAAG 
GCTTGTGAGC CCGGAGTCGA CTATGTGTAC AAGACCGAGC TAACCAACAT AGAGCTGTTG 
GATGATTTTG ATGAGTACAC CATGACCATC CAGCAGGTCA TCAAGTCAGG CTCAGATGAG 
GTGCAGGCAG GGCAGCAACG CAAGTTCATC AGCCACATCA AGTGCAGAAA CGCCCTGAAG 
CTGCAGAAAG GGAAGAAGTA CCTCATGTGG GGCCTCTCCT CTGACCTCTG GGGAGAAAAG 
CCCAACACCA GCTACATCAT TGGGAAGGAC ACGTGGGTGG AGCACTGGCC TGAGGCAGAA 
GAATGCCAGG ATCAGAAGTA CCAGAAACAG TGCGAAGAAC TTGGGGCATT CACAGAATCT 
ATGGTGGTTT ATGGTTGTCC CAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C3 ( NM_009778.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033908.2 Mus musculus complement component 3 (C3), mRNA.

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CloneID	OMu22069
Clone ID Related Accession (Same CDS sequence)	NM_009778.3
Accession Version	NM_009778.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	complement C3 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CT025692.15. On Mar 21, 2015 this sequence version replaced NM_009778.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: K02782.1, BC043338.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGACCAG CTTCAGGGTC CCAGCTACTA GTGCTACTGC TGCTGTTGGC CAGCTCCCCA 
TTAGCTCTGG GGATCCCCAT GTATTCCATC ATTACTCCCA ATGTCCTACG GCTGGAGAGC 
GAAGAGACCA TCGTACTGGA GGCCCACGAT GCTCAGGGTG ACATCCCAGT CACAGTCACT 
GTGCAAGACT TCCTAAAGAG GCAAGTGCTG ACCAGTGAGA AGACAGTGTT GACAGGAGCC 
AGTGGACATC TGAGAAGCGT CTCCATCAAG ATTCCAGCCA GTAAGGAATT CAACTCAGAT 
AAGGAGGGGC ACAAGTACGT GACAGTGGTG GCAAACTTCG GGGAAACGGT GGTGGAGAAA 
GCAGTGATGG TAAGCTTCCA GAGTGGGTAC CTCTTCATCC AGACAGACAA GACCATCTAC 
ACCCCTGGCT CCACTGTCTT ATATCGGATC TTCACTGTGG ACAACAACCT ACTGCCCGTG 
GGCAAGACAG TCGTCATCCT CATTGAGACC CCCGATGGCA TTCCTGTCAA GAGAGACATT 
CTGTCTTCCA ACAACCAACA CGGCATCTTG CCTTTGTCTT GGAACATTCC TGAACTGGTC 
AACATGGGGC AGTGGAAGAT CCGAGCCTTT TACGAACATG CGCCGAAGCA GATCTTCTCC 
GCAGAGTTTG AGGTGAAGGA ATACGTGCTG CCCAGTTTTG AGGTCCGGGT GGAGCCCACA 
GAGACATTTT ATTACATCGA TGACCCAAAT GGCCTGGAAG TTTCCATCAT AGCCAAGTTC 
CTGTACGGGA AAAACGTGGA CGGGACAGCC TTCGTGATTT TTGGGGTCCA GGATGGCGAT 
AAGAAGATTT CTCTGGCCCA CTCCCTCACG CGCGTAGTGA TTGAGGATGG TGTGGGGGAT 
GCAGTGCTGA CCCGGAAGGT GCTGATGGAG GGGGTACGGC CTTCCAACGC CGACGCCCTG 
GTGGGGAAGT CCCTGTATGT CTCCGTCACT GTCATCCTGC ACTCAGGTAG TGACATGGTA 
GAGGCAGAGC GCAGTGGGAT CCCGATTGTC ACTTCCCCGT ACCAGATCCA CTTCACCAAG 
ACACCCAAAT TCTTCAAGCC AGCCATGCCC TTTGACCTCA TGGTGTTCGT GACCAACCCC 
GATGGCTCTC CGGCCAGCAA AGTGCTGGTG GTCACTCAGG GATCTAATGC AAAGGCTCTC 
ACCCAAGATG ATGGCGTGGC CAAGCTAAGC ATCAACACAC CCAACAGCCG CCAACCCCTG 
ACCATCACAG TCCGCACCAA GAAGGACACT CTCCCAGAAT CACGGCAGGC CACCAAGACA 
ATGGAGGCCC ATCCCTACAG CACTATGCAC AACTCCAACA ACTACCTACA CTTGTCAGTG 
TCACGAATGG AGCTCAAGCC GGGGGACAAC CTCAATGTCA ACTTCCACCT GCGCACAGAC 
CCAGGCCATG AGGCCAAGAT CCGATACTAC ACCTACCTGG TTATGAACAA GGGGAAGCTC 
CTGAAGGCAG GCCGCCAGGT TCGGGAGCCT GGCCAGGACC TGGTGGTCTT GTCCCTGCCC 
ATCACTCCAG AGTTTATTCC TTCATTTCGC CTGGTGGCTT ACTACACCCT GATTGGAGCT 
AGTGGCCAGA GGGAGGTGGT GGCTGACTCT GTGTGGGTGG ATGTGAAGGA TTCCTGTATT 
GGCACGCTGG TGGTGAAGGG TGACCCAAGA GATAACCATC TCGCACCTGG GCAACAAACG 
ACACTCAGGA TTGAAGGAAA CCAGGGGGCC CGAGTGGGGC TAGTGGCTGT GGACAAGGGA 
GTGTTTGTGC TGAACAAGAA GAACAAACTC ACACAGAGCA AGATCTGGGA TGTGGTAGAG 
AAGGCAGACA TTGGCTGCAC CCCAGGCAGT GGGAAGAACT ATGCTGGTGT CTTCATGGAT 
GCAGGCCTGG CCTTCAAGAC AAGCCAAGGA CTGCAGACTG AACAGAGAGC AGATCTTGAG 
TGCACCAAGC CAGCAGCCCG CCGCCGTCGC TCAGTACAGT TGATGGAAAG AAGGATGGAC 
AAAGCTGGTC AGTACACTGA CAAGGGTCTT CGGAAGTGTT GTGAGGATGG TATGCGGGAT 
ATCCCTATGA GATACAGCTG CCAGCGCCGG GCACGCCTCA TCACCCAGGG CGAGAACTGC 
ATAAAGGCCT TCATAGACTG CTGCAACCAC ATCACCAAGC TGCGTGAACA ACACAGAAGA 
GACCACGTGC TGGGCCTGGC CAGGAGTGAA TTGGAGGAAG ACATAATTCC AGAAGAAGAT 
ATTATCTCTA GAAGCCACTT CCCACAGAGC TGGTTGTGGA CCATAGAAGA GTTGAAAGAA 
CCAGAGAAAA ATGGAATCTC TACGAAGGTC ATGAACATCT TTCTCAAAGA TTCCATCACC 
ACCTGGGAGA TTCTGGCAGT GAGCTTGTCA GACAAGAAAG GGATCTGTGT GGCAGACCCC 
TATGAGATCA GAGTGATGCA GGACTTCTTC ATTGACCTGC GGCTGCCCTA CTCTGTAGTG 
CGCAACGAAC AGGTGGAGAT CAGAGCTGTG CTCTTCAACT ACCGTGAACA GGAGGAACTT 
AAGGTGAGGG TGGAACTGTT GCATAATCCA GCCTTCTGCA GCATGGCCAC CGCCAAGAAT 
CGCTACTTCC AGACCATCAA AATCCCTCCC AAGTCCTCGG TGGCTGTACC GTATGTCATT 
GTCCCCTTGA AGATCGGCCA ACAAGAGGTG GAGGTCAAGG CTGCTGTCTT CAATCACTTC 
ATCAGTGATG GTGTCAAGAA GACACTGAAG GTCGTGCCAG AAGGAATGAG AATCAACAAA 
ACTGTGGCCA TCCATACACT GGACCCAGAG AAGCTCGGTC AAGGGGGAGT GCAGAAGGTG 
GATGTGCCTG CCGCAGACCT TAGCGACCAA GTGCCAGACA CAGACTCTGA GACCAGAATT 
ATCCTGCAAG GGAGCCCGGT GGTTCAGATG GCTGAAGATG CTGTGGACGG GGAGCGGCTG 
AAACACCTGA TCGTGACCCC CGCAGGCTGT GGGGAACAGA ACATGATTGG CATGACACCA 
ACAGTCATTG CGGTACACTA CCTGGACCAG ACCGAACAGT GGGAGAAGTT CGGCATAGAG 
AAGAGGCAAG AGGCCCTGGA GCTCATCAAG AAAGGGTACA CCCAGCAGCT GGCCTTCAAA 
CAGCCCAGCT CTGCCTATGC TGCCTTCAAC AACCGGCCCC CCAGCACCTG GCTGACAGCC 
TACGTGGTCA AGGTCTTCTC TCTAGCTGCC AACCTCATCG CCATCGACTC TCACGTCCTG 
TGTGGGGCTG TTAAATGGTT GATTCTGGAG AAACAGAAGC CGGATGGTGT CTTTCAGGAG 
GATGGGCCCG TGATTCACCA AGAAATGATT GGTGGCTTCC GGAACGCCAA GGAGGCAGAT 
GTGTCACTCA CAGCCTTCGT CCTCATCGCA CTGCAGGAAG CCAGGGACAT CTGTGAGGGG 
CAGGTCAATA GCCTTCCTGG GAGCATCAAC AAGGCAGGGG AGTATATTGA AGCCAGTTAC 
ATGAACCTGC AGAGACCATA CACAGTGGCC ATTGCTGGGT ATGCCCTGGC CCTGATGAAC 
AAACTGGAGG AACCTTACCT CGGCAAGTTT CTGAACACAG CCAAAGATCG GAACCGCTGG 
GAGGAGCCTG ACCAGCAGCT CTACAACGTA GAGGCCACAT CCTACGCCCT CCTGGCCCTG 
CTGCTGCTGA AAGACTTTGA CTCTGTGCCC CCTGTAGTGC GCTGGCTCAA TGAGCAAAGA 
TACTACGGAG GCGGCTATGG CTCCACCCAG GCTACCTTCA TGGTATTCCA AGCCTTGGCC 
CAATATCAAA CAGATGTCCC TGACCATAAG GACTTGAACA TGGATGTGTC CTTCCACCTC 
CCCAGCCGTA GCTCTGCAAC CACGTTTCGC CTGCTCTGGG AAAATGGCAA CCTCCTGCGA 
TCGGAAGAGA CCAAGCAAAA TGAGGCCTTC TCTCTAACAG CCAAAGGAAA AGGCCGAGGC 
ACATTGTCGG TGGTGGCAGT GTATCATGCC AAACTCAAAA GCAAAGTCAC CTGCAAGAAG 
TTTGACCTCA GGGTCAGCAT AAGACCAGCC CCTGAGACAG CCAAGAAGCC CGAGGAAGCC 
AAGAATACCA TGTTCCTTGA AATCTGCACC AAGTACTTGG GAGATGTGGA CGCCACTATG 
TCCATCCTGG ACATCTCCAT GATGACTGGC TTTGCTCCAG ACACAAAGGA CCTGGAACTG 
CTGGCCTCTG GAGTAGATAG ATACATCTCC AAGTACGAGA TGAACAAAGC CTTCTCCAAC 
AAGAACACCC TCATCATCTA CCTAGAAAAG ATTTCACACA CCGAAGAAGA CTGCCTGACC 
TTCAAAGTTC ACCAGTACTT TAATGTGGGA CTTATCCAGC CCGGGTCGGT CAAGGTCTAC 
TCCTATTACA ACCTCGAGGA ATCATGCACC CGGTTCTATC ATCCAGAGAA GGACGATGGG 
ATGCTCAGCA AGCTGTGCCA CAGTGAAATG TGCCGGTGTG CTGAAGAGAA CTGCTTCATG 
CAACAGTCAC AGGAGAAGAT CAACCTGAAT GTCCGGCTAG ACAAGGCTTG TGAGCCCGGA 
GTCGACTATG TGTACAAGAC CGAGCTAACC AACATAGAGC TGTTGGATGA TTTTGATGAG 
TACACCATGA CCATCCAGCA GGTCATCAAG TCAGGCTCAG ATGAGGTGCA GGCAGGGCAG 
CAACGCAAGT TCATCAGCCA CATCAAGTGC AGAAACGCCC TGAAGCTGCA GAAAGGGAAG 
AAGTACCTCA TGTGGGGCCT CTCCTCTGAC CTCTGGGGAG AAAAGCCCAA CACCAGCTAC 
ATCATTGGGA AGGACACGTG GGTGGAGCAC TGGCCTGAGG CAGAAGAATG CCAGGATCAG 
AAGTACCAGA AACAGTGCGA AGAACTTGGG GCATTCACAG AATCTATGGT GGTTTATGGT 
TGTCCCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033908.2
CDS	102..5093
Translation	MGPASGSQLLVLLLLLASSPLALGIPMYSIITPNVLRLESEETIVLEAHDAQGDIPVTVTVQDFLKRQVLTSEKTVLTGASGHLRSVSIKIPASKEFNSDKEGHKYVTVVANFGETVVEKAVMVSFQSGYLFIQTDKTIYTPGSTVLYRIFTVDNNLLPVGKTVVILIETPDGIPVKRDILSSNNQHGILPLSWNIPELVNMGQWKIRAFYEHAPKQIFSAEFEVKEYVLPSFEVRVEPTETFYYIDDPNGLEVSIIAKFLYGKNVDGTAFVIFGVQDGDKKISLAHSLTRVVIEDGVGDAVLTRKVLMEGVRPSNADALVGKSLYVSVTVILHSGSDMVEAERSGIPIVTSPYQIHFTKTPKFFKPAMPFDLMVFVTNPDGSPASKVLVVTQGSNAKALTQDDGVAKLSINTPNSRQPLTITVRTKKDTLPESRQATKTMEAHPYSTMHNSNNYLHLSVSRMELKPGDNLNVNFHLRTDPGHEAKIRYYTYLVMNKGKLLKAGRQVREPGQDLVVLSLPITPEFIPSFRLVAYYTLIGASGQREVVADSVWVDVKDSCIGTLVVKGDPRDNHLAPGQQTTLRIEGNQGARVGLVAVDKGVFVLNKKNKLTQSKIWDVVEKADIGCTPGSGKNYAGVFMDAGLAFKTSQGLQTEQRADLECTKPAARRRRSVQLMERRMDKAGQYTDKGLRKCCEDGMRDIPMRYSCQRRARLITQGENCIKAFIDCCNHITKLREQHRRDHVLGLARSELEEDIIPEEDIISRSHFPQSWLWTIEELKEPEKNGISTKVMNIFLKDSITTWEILAVSLSDKKGICVADPYEIRVMQDFFIDLRLPYSVVRNEQVEIRAVLFNYREQEELKVRVELLHNPAFCSMATAKNRYFQTIKIPPKSSVAVPYVIVPLKIGQQEVEVKAAVFNHFISDGVKKTLKVVPEGMRINKTVAIHTLDPEKLGQGGVQKVDVPAADLSDQVPDTDSETRIILQGSPVVQMAEDAVDGERLKHLIVTPAGCGEQNMIGMTPTVIAVHYLDQTEQWEKFGIEKRQEALELIKKGYTQQLAFKQPSSAYAAFNNRPPSTWLTAYVVKVFSLAANLIAIDSHVLCGAVKWLILEKQKPDGVFQEDGPVIHQEMIGGFRNAKEADVSLTAFVLIALQEARDICEGQVNSLPGSINKAGEYIEASYMNLQRPYTVAIAGYALALMNKLEEPYLGKFLNTAKDRNRWEEPDQQLYNVEATSYALLALLLLKDFDSVPPVVRWLNEQRYYGGGYGSTQATFMVFQALAQYQTDVPDHKDLNMDVSFHLPSRSSATTFRLLWENGNLLRSEETKQNEAFSLTAKGKGRGTLSVVAVYHAKLKSKVTCKKFDLRVSIRPAPETAKKPEEAKNTMFLEICTKYLGDVDATMSILDISMMTGFAPDTKDLELLASGVDRYISKYEMNKAFSNKNTLIIYLEKISHTEEDCLTFKVHQYFNVGLIQPGSVKVYSYYNLEESCTRFYHPEKDDGMLSKLCHSEMCRCAEENCFMQQSQEKINLNVRLDKACEPGVDYVYKTELTNIELLDDFDEYTMTIQQVIKSGSDEVQAGQQRKFISHIKCRNALKLQKGKKYLMWGLSSDLWGEKPNTSYIIGKDTWVEHWPEAEECQDQKYQKQCEELGAFTESMVVYGCPN

Target ORF information:

RefSeq Version	NM_009778.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus complement component 3 (C3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009778.3

ORF Insert Sequence:

ATGGGACCAG CTTCAGGGTC CCAGCTACTA GTGCTACTGC TGCTGTTGGC CAGCTCCCCA 
TTAGCTCTGG GGATCCCCAT GTATTCCATC ATTACTCCCA ATGTCCTACG GCTGGAGAGC 
GAAGAGACCA TCGTACTGGA GGCCCACGAT GCTCAGGGTG ACATCCCAGT CACAGTCACT 
GTGCAAGACT TCCTAAAGAG GCAAGTGCTG ACCAGTGAGA AGACAGTGTT GACAGGAGCC 
AGTGGACATC TGAGAAGCGT CTCCATCAAG ATTCCAGCCA GTAAGGAATT CAACTCAGAT 
AAGGAGGGGC ACAAGTACGT GACAGTGGTG GCAAACTTCG GGGAAACGGT GGTGGAGAAA 
GCAGTGATGG TAAGCTTCCA GAGTGGGTAC CTCTTCATCC AGACAGACAA GACCATCTAC 
ACCCCTGGCT CCACTGTCTT ATATCGGATC TTCACTGTGG ACAACAACCT ACTGCCCGTG 
GGCAAGACAG TCGTCATCCT CATTGAGACC CCCGATGGCA TTCCTGTCAA GAGAGACATT 
CTGTCTTCCA ACAACCAACA CGGCATCTTG CCTTTGTCTT GGAACATTCC TGAACTGGTC 
AACATGGGGC AGTGGAAGAT CCGAGCCTTT TACGAACATG CGCCGAAGCA GATCTTCTCC 
GCAGAGTTTG AGGTGAAGGA ATACGTGCTG CCCAGTTTTG AGGTCCGGGT GGAGCCCACA 
GAGACATTTT ATTACATCGA TGACCCAAAT GGCCTGGAAG TTTCCATCAT AGCCAAGTTC 
CTGTACGGGA AAAACGTGGA CGGGACAGCC TTCGTGATTT TTGGGGTCCA GGATGGCGAT 
AAGAAGATTT CTCTGGCCCA CTCCCTCACG CGCGTAGTGA TTGAGGATGG TGTGGGGGAT 
GCAGTGCTGA CCCGGAAGGT GCTGATGGAG GGGGTACGGC CTTCCAACGC CGACGCCCTG 
GTGGGGAAGT CCCTGTATGT CTCCGTCACT GTCATCCTGC ACTCAGGTAG TGACATGGTA 
GAGGCAGAGC GCAGTGGGAT CCCGATTGTC ACTTCCCCGT ACCAGATCCA CTTCACCAAG 
ACACCCAAAT TCTTCAAGCC AGCCATGCCC TTTGACCTCA TGGTGTTCGT GACCAACCCC 
GATGGCTCTC CGGCCAGCAA AGTGCTGGTG GTCACTCAGG GATCTAATGC AAAGGCTCTC 
ACCCAAGATG ATGGCGTGGC CAAGCTAAGC ATCAACACAC CCAACAGCCG CCAACCCCTG 
ACCATCACAG TCCGCACCAA GAAGGACACT CTCCCAGAAT CACGGCAGGC CACCAAGACA 
ATGGAGGCCC ATCCCTACAG CACTATGCAC AACTCCAACA ACTACCTACA CTTGTCAGTG 
TCACGAATGG AGCTCAAGCC GGGGGACAAC CTCAATGTCA ACTTCCACCT GCGCACAGAC 
CCAGGCCATG AGGCCAAGAT CCGATACTAC ACCTACCTGG TTATGAACAA GGGGAAGCTC 
CTGAAGGCAG GCCGCCAGGT TCGGGAGCCT GGCCAGGACC TGGTGGTCTT GTCCCTGCCC 
ATCACTCCAG AGTTTATTCC TTCATTTCGC CTGGTGGCTT ACTACACCCT GATTGGAGCT 
AGTGGCCAGA GGGAGGTGGT GGCTGACTCT GTGTGGGTGG ATGTGAAGGA TTCCTGTATT 
GGCACGCTGG TGGTGAAGGG TGACCCAAGA GATAACCATC TCGCACCTGG GCAACAAACG 
ACACTCAGGA TTGAAGGAAA CCAGGGGGCC CGAGTGGGGC TAGTGGCTGT GGACAAGGGA 
GTGTTTGTGC TGAACAAGAA GAACAAACTC ACACAGAGCA AGATCTGGGA TGTGGTAGAG 
AAGGCAGACA TTGGCTGCAC CCCAGGCAGT GGGAAGAACT ATGCTGGTGT CTTCATGGAT 
GCAGGCCTGG CCTTCAAGAC AAGCCAAGGA CTGCAGACTG AACAGAGAGC AGATCTTGAG 
TGCACCAAGC CAGCAGCCCG CCGCCGTCGC TCAGTACAGT TGATGGAAAG AAGGATGGAC 
AAAGCTGGTC AGTACACTGA CAAGGGTCTT CGGAAGTGTT GTGAGGATGG TATGCGGGAT 
ATCCCTATGA GATACAGCTG CCAGCGCCGG GCACGCCTCA TCACCCAGGG CGAGAACTGC 
ATAAAGGCCT TCATAGACTG CTGCAACCAC ATCACCAAGC TGCGTGAACA ACACAGAAGA 
GACCACGTGC TGGGCCTGGC CAGGAGTGAA TTGGAGGAAG ACATAATTCC AGAAGAAGAT 
ATTATCTCTA GAAGCCACTT CCCACAGAGC TGGTTGTGGA CCATAGAAGA GTTGAAAGAA 
CCAGAGAAAA ATGGAATCTC TACGAAGGTC ATGAACATCT TTCTCAAAGA TTCCATCACC 
ACCTGGGAGA TTCTGGCAGT GAGCTTGTCA GACAAGAAAG GGATCTGTGT GGCAGACCCC 
TATGAGATCA GAGTGATGCA GGACTTCTTC ATTGACCTGC GGCTGCCCTA CTCTGTAGTG 
CGCAACGAAC AGGTGGAGAT CAGAGCTGTG CTCTTCAACT ACCGTGAACA GGAGGAACTT 
AAGGTGAGGG TGGAACTGTT GCATAATCCA GCCTTCTGCA GCATGGCCAC CGCCAAGAAT 
CGCTACTTCC AGACCATCAA AATCCCTCCC AAGTCCTCGG TGGCTGTACC GTATGTCATT 
GTCCCCTTGA AGATCGGCCA ACAAGAGGTG GAGGTCAAGG CTGCTGTCTT CAATCACTTC 
ATCAGTGATG GTGTCAAGAA GACACTGAAG GTCGTGCCAG AAGGAATGAG AATCAACAAA 
ACTGTGGCCA TCCATACACT GGACCCAGAG AAGCTCGGTC AAGGGGGAGT GCAGAAGGTG 
GATGTGCCTG CCGCAGACCT TAGCGACCAA GTGCCAGACA CAGACTCTGA GACCAGAATT 
ATCCTGCAAG GGAGCCCGGT GGTTCAGATG GCTGAAGATG CTGTGGACGG GGAGCGGCTG 
AAACACCTGA TCGTGACCCC CGCAGGCTGT GGGGAACAGA ACATGATTGG CATGACACCA 
ACAGTCATTG CGGTACACTA CCTGGACCAG ACCGAACAGT GGGAGAAGTT CGGCATAGAG 
AAGAGGCAAG AGGCCCTGGA GCTCATCAAG AAAGGGTACA CCCAGCAGCT GGCCTTCAAA 
CAGCCCAGCT CTGCCTATGC TGCCTTCAAC AACCGGCCCC CCAGCACCTG GCTGACAGCC 
TACGTGGTCA AGGTCTTCTC TCTAGCTGCC AACCTCATCG CCATCGACTC TCACGTCCTG 
TGTGGGGCTG TTAAATGGTT GATTCTGGAG AAACAGAAGC CGGATGGTGT CTTTCAGGAG 
GATGGGCCCG TGATTCACCA AGAAATGATT GGTGGCTTCC GGAACGCCAA GGAGGCAGAT 
GTGTCACTCA CAGCCTTCGT CCTCATCGCA CTGCAGGAAG CCAGGGACAT CTGTGAGGGG 
CAGGTCAATA GCCTTCCTGG GAGCATCAAC AAGGCAGGGG AGTATATTGA AGCCAGTTAC 
ATGAACCTGC AGAGACCATA CACAGTGGCC ATTGCTGGGT ATGCCCTGGC CCTGATGAAC 
AAACTGGAGG AACCTTACCT CGGCAAGTTT CTGAACACAG CCAAAGATCG GAACCGCTGG 
GAGGAGCCTG ACCAGCAGCT CTACAACGTA GAGGCCACAT CCTACGCCCT CCTGGCCCTG 
CTGCTGCTGA AAGACTTTGA CTCTGTGCCC CCTGTAGTGC GCTGGCTCAA TGAGCAAAGA 
TACTACGGAG GCGGCTATGG CTCCACCCAG GCTACCTTCA TGGTATTCCA AGCCTTGGCC 
CAATATCAAA CAGATGTCCC TGACCATAAG GACTTGAACA TGGATGTGTC CTTCCACCTC 
CCCAGCCGTA GCTCTGCAAC CACGTTTCGC CTGCTCTGGG AAAATGGCAA CCTCCTGCGA 
TCGGAAGAGA CCAAGCAAAA TGAGGCCTTC TCTCTAACAG CCAAAGGAAA AGGCCGAGGC 
ACATTGTCGG TGGTGGCAGT GTATCATGCC AAACTCAAAA GCAAAGTCAC CTGCAAGAAG 
TTTGACCTCA GGGTCAGCAT AAGACCAGCC CCTGAGACAG CCAAGAAGCC CGAGGAAGCC 
AAGAATACCA TGTTCCTTGA AATCTGCACC AAGTACTTGG GAGATGTGGA CGCCACTATG 
TCCATCCTGG ACATCTCCAT GATGACTGGC TTTGCTCCAG ACACAAAGGA CCTGGAACTG 
CTGGCCTCTG GAGTAGATAG ATACATCTCC AAGTACGAGA TGAACAAAGC CTTCTCCAAC 
AAGAACACCC TCATCATCTA CCTAGAAAAG ATTTCACACA CCGAAGAAGA CTGCCTGACC 
TTCAAAGTTC ACCAGTACTT TAATGTGGGA CTTATCCAGC CCGGGTCGGT CAAGGTCTAC 
TCCTATTACA ACCTCGAGGA ATCATGCACC CGGTTCTATC ATCCAGAGAA GGACGATGGG 
ATGCTCAGCA AGCTGTGCCA CAGTGAAATG TGCCGGTGTG CTGAAGAGAA CTGCTTCATG 
CAACAGTCAC AGGAGAAGAT CAACCTGAAT GTCCGGCTAG ACAAGGCTTG TGAGCCCGGA 
GTCGACTATG TGTACAAGAC CGAGCTAACC AACATAGAGC TGTTGGATGA TTTTGATGAG 
TACACCATGA CCATCCAGCA GGTCATCAAG TCAGGCTCAG ATGAGGTGCA GGCAGGGCAG 
CAACGCAAGT TCATCAGCCA CATCAAGTGC AGAAACGCCC TGAAGCTGCA GAAAGGGAAG 
AAGTACCTCA TGTGGGGCCT CTCCTCTGAC CTCTGGGGAG AAAAGCCCAA CACCAGCTAC 
ATCATTGGGA AGGACACGTG GGTGGAGCAC TGGCCTGAGG CAGAAGAATG CCAGGATCAG 
AAGTACCAGA AACAGTGCGA AGAACTTGGG GCATTCACAG AATCTATGGT GGTTTATGGT 
TGTCCCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Acylation-stimulating protein deficiency and altered adipose tissue in alternative complement pathway knockout mice.
American journal of physiology. Endocrinology and metabolism294(3)E521-9(2008 Mar)
Paglialunga S,Fisette A,Yan Y,Deshaies Y,Brouillette JF,Pekna M,Cianflone K

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C3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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