LOC103909734 cDNA ORF clone, Danio rerio(Zebrafish)

The following LOC103909734 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103909734 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa52450 XM_009297213.2
Latest version!
Danio rerio E3 ubiquitin-protein ligase HECW1-like (LOC103909734), mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $510.30
$729.00
ODa151754 XM_021470254.1
Latest version!
Danio rerio E3 ubiquitin-protein ligase HECW1-like (LOC103909734), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa52450
    Clone ID Related Accession (Same CDS sequence) XM_009297213.2
    Accession Version XM_009297213.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2805bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HECW1-like
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297213.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGATCCACC GGCGGATCTT TCTGTCTCAG AACCTGTACC AGAGCAGGTT TGTGAGTCTG 
    GCGGCGATGG CGTCTCCGTC GCGCAGTTCT CCGATGCGCC GGCGCTGGAA AGATGGTTCT
    CGATTCGGTT TCGCTCAGAC AGACCGATCG CTCCATCGCT CAACCAGTGA CACCGACCTG
    CTGACCTTAC AGAGCCGCTC CACACTGACC GTAAGCTCCT CCCAATACTG CATCGGCCAA
    TCAGAGGACA TCTGCATCTG CTGGGACATC AAAGAGGAGG TGGAGCCTGG AGACTGGATC
    GGCATGTACC TCATCGATGA GGTTTTGTCT GAGAATTTCC TGGACTATAA GAGTCGTGGT
    GTGAGCGGAT CCCACAAGGG CCAGATCAGA TGGAGGATCG ACGCCAGCCT CTACTTCATG
    GAGGCTGAGA CGAAGATTTG CTTCAAATAT TATCATGGTT TGAGTGGAGC CCTCAGAGCC
    ACAACACCCA GTGTGACGGT CAGGAACACG TCCGCCTCTG CGCTGAAGCC GGTCATCAGT
    CCGGATCTCC CTCAGTCTCT GGGCAGCAGG AGACTCATCA GCTTCTCCCT CTCCGACGTG
    CAGGCGGTGG GTCTGAAGAA GGGGATGTTC TTTAATCCAG ACCCGTATCT GAAGATCTCC
    ATCCAGCCTG GAAAGCAGAG TCTGTACCCT GCGCTTCCAC ACCACGGCCA GGAGAAACGC
    TCTAGCATCG CCTGCAACAC CATCAGCCCC GTCTGGAAGA GAGAGCGGTT TAACTTTGTG
    TCGCTGCCGA CGGATGTTTT GGAGATCGAG GTTAAAGATA AGTTTGCGAA GAGTCGACCC
    ATTATTAAAC GCTTTCTGGG GAAGCTGGTG GTGCCGGTGC AGAGGCTGCT GGAGAAACAC
    GCCATCGGGG ACCGTGTGGT GAGCTACACT TTAGGTCGGC GGCTTCCAAC AGATCATGTC
    AGCGGCCAGC TGCAGTTTCG CTTCGAGATA ACTTCATCAA TCCATCCAGA TGATGAGGAA
    GTGTCTCTCA ATGCAATGCC AGTCTTTGAT GCGGAGCCAG ACCAGCAGAC AGATCCGGAG
    CCAGTTGAAG ATCCACCACC AGCTTCTGAT CCACCAATGG AGGATGCATT GAGTCTAGAC
    GTTCCCGAGC CTCCACCCGA ATGTCCTCCA GAAATCGAGA TGCGGGATGA TGTTGATGTT
    CAGCTTACAT CTCTATCAGA ACCCAGTAAG GAAACCCAAC CCGAAGCAGA TGAGGAGATG
    GTTACAGCTC AAGGAGATAC ACCAATGAAT GATACAACTC TTGGAGAAAC TGCAGAACAA
    GACACTGGTA TAATGGGGGA TCATGGAGAG ACGCTGGAAG AAACTATGGC TGAGACTCCA
    TCGGAAGTAC TGGACAACCG TGATGCTGAA CATACTGATG GAGTAAATGA AGAGCATGAA
    GTAGAAACAA GCTCTGAACA GGTGGATCTA ATGGTAGACG GAGATAATGA ATGTTCCCCA
    AGGGTACGAG TTCAGTTAAA GCGCAAGAAT CGACCGTGCT CGCTTCCAGT ATCTGAATTA
    GAGACGGTTA TCGCCTCGAC GTGTGAAGAA CCCGAAACGC CGCGCTCTCA TTACATCCGC
    ATTCATCATC TACTCCACAG TCTGCCGTCA GCTCAGTGCC ACCGAGATGA AGAGGAAGAT
    GCACCTGATC CCGAATCTTC AGAAGAAATC ACGATCAAAC CTCAAGAAGA TAAAGAAGAC
    GAAGATGAGG TATCCGAAGC TCAGTCTCCG TCAGTTGTTC CTGAGTGTCC GAGACCGTGT
    TGCAGGCGAA TTTTACCACG TAGCCTCTCA ATCGAGCGTC TTTCTGAACT CAACCAGCTT
    CTGGAGGGAG AAAGACCGTC TCCGTCCACC CTCCGGATAT ACAGTGAAGA CGGGGATTCG
    GGAAGAAGAA GGGAGCCTCG GGAAAGCGAG TGTGAATTTT GTGATAACTC CTGCTATAGT
    ACGTCATGTT ACAGCACGTC CTGTTACAGC ACCTCGTGTT ACAGCACAAA CTCGGGTTAC
    GAGGGCCGAA ACCGGCTGTG CAGCCACACA CGCCTGTCCT CGGTGGACAG CACCCGCCTG
    TCGGAGAGCA CGGTTTTCTC TTCGCAAGAG GAAGAGGAGG AGGAAAACAG CGCGTTTGAG
    TCGGTGTCAG ACTCCATGCG GAGCCCAGAT GTGCGGGAGC CAGGCGGAGG CTCGGTCCAG
    TGGAGGGAGT CTTCTTCAGA AGAGAGTCCT CTGGGGGACG CGGAGGTGGA TGAGTCGCCT
    GTGGCGGGAC CTAGTGTTCG GCCAACAGGC AGTTTTCATT TGCACTGCAG TCATCATGCA
    ATCACTCAGA CTCCTGCTTT GCGGCCTGAT CCCCATCATT ACCCCAGTGT TGATCAACCA
    TTACCACCAA ATTGGGAGGC GCGTGTGGAC AGTCACGGTC GTGTTTTTTA CGTGGATCAT
    GTAAACCGGA CAACAACATG GCAGAGACCC ATTCATGCAG CCAAGCGCAC CGGCATGCAG
    AGATCAGGGT CCACACACCA GATGGAGCAG CTCAACCGCA GGTATCAGAA TATTCAGCGG
    ACGATGGCGA GCGAAGACGA GCCTGCAAAC ACTCGCGGAT CTGAGAGGAA CACTGGAGAT
    ACCGAGAGCG AATCCACAAC ACAATCCACA GATCAGAGAA GAGACGGCCC TTCATCTCCG
    GTCAATAACC CGAACGTCTC GCAGCTGCTT CAGTGTCCCG CCGTCAAGTT CATCACTCAC
    CCTGAATTCT TCACTGTCCT CCATGCAAAC TACGAGCAGC TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295488.1
    CDS292..3096
    Translation

    Target ORF information:

    RefSeq Version XM_009297213.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio E3 ubiquitin-protein ligase HECW1-like (LOC103909734), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297213.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGATCCACC GGCGGATCTT TCTGTCTCAG AACCTGTACC AGAGCAGGTT TGTGAGTCTG 
    GCGGCGATGG CGTCTCCGTC GCGCAGTTCT CCGATGCGCC GGCGCTGGAA AGATGGTTCT
    CGATTCGGTT TCGCTCAGAC AGACCGATCG CTCCATCGCT CAACCAGTGA CACCGACCTG
    CTGACCTTAC AGAGCCGCTC CACACTGACC GTAAGCTCCT CCCAATACTG CATCGGCCAA
    TCAGAGGACA TCTGCATCTG CTGGGACATC AAAGAGGAGG TGGAGCCTGG AGACTGGATC
    GGCATGTACC TCATCGATGA GGTTTTGTCT GAGAATTTCC TGGACTATAA GAGTCGTGGT
    GTGAGCGGAT CCCACAAGGG CCAGATCAGA TGGAGGATCG ACGCCAGCCT CTACTTCATG
    GAGGCTGAGA CGAAGATTTG CTTCAAATAT TATCATGGTT TGAGTGGAGC CCTCAGAGCC
    ACAACACCCA GTGTGACGGT CAGGAACACG TCCGCCTCTG CGCTGAAGCC GGTCATCAGT
    CCGGATCTCC CTCAGTCTCT GGGCAGCAGG AGACTCATCA GCTTCTCCCT CTCCGACGTG
    CAGGCGGTGG GTCTGAAGAA GGGGATGTTC TTTAATCCAG ACCCGTATCT GAAGATCTCC
    ATCCAGCCTG GAAAGCAGAG TCTGTACCCT GCGCTTCCAC ACCACGGCCA GGAGAAACGC
    TCTAGCATCG CCTGCAACAC CATCAGCCCC GTCTGGAAGA GAGAGCGGTT TAACTTTGTG
    TCGCTGCCGA CGGATGTTTT GGAGATCGAG GTTAAAGATA AGTTTGCGAA GAGTCGACCC
    ATTATTAAAC GCTTTCTGGG GAAGCTGGTG GTGCCGGTGC AGAGGCTGCT GGAGAAACAC
    GCCATCGGGG ACCGTGTGGT GAGCTACACT TTAGGTCGGC GGCTTCCAAC AGATCATGTC
    AGCGGCCAGC TGCAGTTTCG CTTCGAGATA ACTTCATCAA TCCATCCAGA TGATGAGGAA
    GTGTCTCTCA ATGCAATGCC AGTCTTTGAT GCGGAGCCAG ACCAGCAGAC AGATCCGGAG
    CCAGTTGAAG ATCCACCACC AGCTTCTGAT CCACCAATGG AGGATGCATT GAGTCTAGAC
    GTTCCCGAGC CTCCACCCGA ATGTCCTCCA GAAATCGAGA TGCGGGATGA TGTTGATGTT
    CAGCTTACAT CTCTATCAGA ACCCAGTAAG GAAACCCAAC CCGAAGCAGA TGAGGAGATG
    GTTACAGCTC AAGGAGATAC ACCAATGAAT GATACAACTC TTGGAGAAAC TGCAGAACAA
    GACACTGGTA TAATGGGGGA TCATGGAGAG ACGCTGGAAG AAACTATGGC TGAGACTCCA
    TCGGAAGTAC TGGACAACCG TGATGCTGAA CATACTGATG GAGTAAATGA AGAGCATGAA
    GTAGAAACAA GCTCTGAACA GGTGGATCTA ATGGTAGACG GAGATAATGA ATGTTCCCCA
    AGGGTACGAG TTCAGTTAAA GCGCAAGAAT CGACCGTGCT CGCTTCCAGT ATCTGAATTA
    GAGACGGTTA TCGCCTCGAC GTGTGAAGAA CCCGAAACGC CGCGCTCTCA TTACATCCGC
    ATTCATCATC TACTCCACAG TCTGCCGTCA GCTCAGTGCC ACCGAGATGA AGAGGAAGAT
    GCACCTGATC CCGAATCTTC AGAAGAAATC ACGATCAAAC CTCAAGAAGA TAAAGAAGAC
    GAAGATGAGG TATCCGAAGC TCAGTCTCCG TCAGTTGTTC CTGAGTGTCC GAGACCGTGT
    TGCAGGCGAA TTTTACCACG TAGCCTCTCA ATCGAGCGTC TTTCTGAACT CAACCAGCTT
    CTGGAGGGAG AAAGACCGTC TCCGTCCACC CTCCGGATAT ACAGTGAAGA CGGGGATTCG
    GGAAGAAGAA GGGAGCCTCG GGAAAGCGAG TGTGAATTTT GTGATAACTC CTGCTATAGT
    ACGTCATGTT ACAGCACGTC CTGTTACAGC ACCTCGTGTT ACAGCACAAA CTCGGGTTAC
    GAGGGCCGAA ACCGGCTGTG CAGCCACACA CGCCTGTCCT CGGTGGACAG CACCCGCCTG
    TCGGAGAGCA CGGTTTTCTC TTCGCAAGAG GAAGAGGAGG AGGAAAACAG CGCGTTTGAG
    TCGGTGTCAG ACTCCATGCG GAGCCCAGAT GTGCGGGAGC CAGGCGGAGG CTCGGTCCAG
    TGGAGGGAGT CTTCTTCAGA AGAGAGTCCT CTGGGGGACG CGGAGGTGGA TGAGTCGCCT
    GTGGCGGGAC CTAGTGTTCG GCCAACAGGC AGTTTTCATT TGCACTGCAG TCATCATGCA
    ATCACTCAGA CTCCTGCTTT GCGGCCTGAT CCCCATCATT ACCCCAGTGT TGATCAACCA
    TTACCACCAA ATTGGGAGGC GCGTGTGGAC AGTCACGGTC GTGTTTTTTA CGTGGATCAT
    GTAAACCGGA CAACAACATG GCAGAGACCC ATTCATGCAG CCAAGCGCAC CGGCATGCAG
    AGATCAGGGT CCACACACCA GATGGAGCAG CTCAACCGCA GGTATCAGAA TATTCAGCGG
    ACGATGGCGA GCGAAGACGA GCCTGCAAAC ACTCGCGGAT CTGAGAGGAA CACTGGAGAT
    ACCGAGAGCG AATCCACAAC ACAATCCACA GATCAGAGAA GAGACGGCCC TTCATCTCCG
    GTCAATAACC CGAACGTCTC GCAGCTGCTT CAGTGTCCCG CCGTCAAGTT CATCACTCAC
    CCTGAATTCT TCACTGTCCT CCATGCAAAC TACGAGCAGC TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151754
    Clone ID Related Accession (Same CDS sequence) XM_021470254.1
    Accession Version XM_021470254.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HECW1-like
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) and transcript sequence (GFIL01020822.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 421 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGATCCACC GGCGGATCTT TCTGTCTCAG AACCTGTACC AGAGCAGGTT TGTGAGTCTG 
    GCGGCGATGG CGTCTCCGTC GCGCAGTTCT CCGATGCGCC GGCGCTGGAA AGATGGTTCT
    CGATTCGGTT TCGCTCAGAC AGACCGATCG CTCCATCGCT CAACCAGTGA CACCGACCTG
    CTGACCTTAC AGAGCCGCTC CACACTGACC GTAAGCTCCT CCCAATACTG CATCGGCCAA
    TCAGAGGACA TCTGCATCTG CTGGGACATC AAAGAGGAGG TGGAGCCTGG AGACTGGATC
    GGCATGTACC TCATCGATGA GGTTTTGTCT GAGAATTTCC TGGACTATAA GAGTCGTGGT
    GTGAGCGGAT CCCACAAGGG CCAGATCAGA TGGAGGATCG ACGCCAGCCT CTACTTCATG
    GAGGCTGAGA CGAAGATTTG CTTCAAATAT TATCATGGTT TGAGTGGAGC CCTCAGAGCC
    ACAACACCCA GTGTGACGGT CAGGAACACG TCCGCCTCTG CGCTGAAGCC GGTCATCAGT
    CCGGATCTCC CTCAGTCTCT GGGCAGCAGG AGACTCATCA GCTTCTCCCT CTCCGACGTG
    CAGGCGGTGG GTCTGAAGAA GGGGATGTTC TTTAATCCAG ACCCGTATCT GAAGATCTCC
    ATCCAGCCTG GAAAGCAGAG TCTGTACCCT GCGCTTCCAC ACCACGGCCA GGAGAAACGC
    TCTAGCATCG CCTGCAACAC CATCAGCCCC GTCTGGAAGA GAGAGCGGTT TAACTTTGTG
    TCGCTGCCGA CGGATGTTTT GGAGATCGAG GTTAAAGATA AGTTTGCGAA GAGTCGACCC
    ATTATTAAAC GCTTTCTGGG GAAGCTGGTG GTGCCGGTGC AGAGGCTGCT GGAGAAACAC
    GCCATCGGGG ACCGTGTGGT GAGCTACACT TTAGGTCGGC GGCTTCCAAC AGATCATGTC
    AGCGGCCAGC TGCAGTTTCG CTTCGAGATA ACTTCATCAA TCCATCCAGA TGATGAGGAA
    GTGTCTCTCA ATGCAATGCC AGTCTTTGAT GCGGAGCCAG ACCAGCAGAC AGATCCGGAG
    CCAGTTGAAG ATCCACCACC AGCTTCTGAT CCACCAATGG AGGATGCATT GAGTCTAGAC
    GTTCCCGAGC CTCCACCCGA ATGTCCTCCA GAAATCGAGA TGCGGGATGA TGTTGATGTT
    CAGCTTACAT CTCTATCAGA ACCCAGTAAG GAAACCCAAC CCGAAGCAGA TGAGGAGATG
    GTTACAGCTC AAGGAGATAC ACCAATGAAT GATACAACTC TTGGAGAAAC TGCAGAACAA
    GACACTGGTA TAATGGGGGA TCATGGAGAG ACGCTGGAAG AAACTATGGC TGAGACTCCA
    TCGGAAGTAC TGGACAACCG TGATGCTGAA CATACTGATG GAGTAAATGA AGAGCATGAA
    GTAGAAACAA GCTCTGAACA GGTGGATCTA ATGGTAGACG GAGATAATGA ATGTTCCCCA
    AGGGTACGAG TTCAGTTAAA GCGCAAGAAT CGACCGTGCT CGCTTCCAGT ATCTGAATTA
    GAGACGGTTA TCGCCTCGAC GTGTGAAGAA CCCGAAACGC CGCGCTCTCA TTACATCCGC
    ATTCATCATC TACTCCACAG TCTGCCGTCA GCTCAGTGCC ACCGAGATGA AGAGGAAGAT
    GCACCTGATC CCGAATCTTC AGAAGAAATC ACGATCAAAC CTCAAGAAGA TAAAGAAGAC
    GAAGATGAGG TATCCGAAGC TCAGTCTCCG TCAGTTGTTC CTGAGTGTCC GAGACCGTGT
    TGCAGGCGAA TTTTACCACG TAGCCTCTCA ATCGAGCGTC TTTCTGAACT CAACCAGCTT
    CTGGAGGGAG AAAGACCGTC TCCGTCCACC CTCCGGATAT ACAGTGAAGA CGGGGATTCG
    GGAAGAAGAA GGGAGCCTCG GGAAAGCGAG TGTGAATTTT GTGATAACTC CTGCTATAGT
    ACGTCATGTT ACAGCACGTC CTGTTACAGC ACCTCGTGTT ACAGCACAAA CTCGGGTTAC
    GAGGGCCGAA ACCGGCTGTG CAGCCACACA CGCCTGTCCT CGGTGGACAG CACCCGCCTG
    TCGGAGAGCA CGGTTTTCTC TTCGCAAGAG GAAGAGGAGG AGGAAAACAG CGCGTTTGAG
    TCGGTGTCAG ACTCCATGCG GAGCCCAGAT GTGCGGGAGC CAGGCGGAGG CTCGGTCCAG
    TGGAGGGAGT CTTCTTCAGA AGAGAGTCCT CTGGGGGACG CGGAGGTGGA TGAGTCGCCT
    GTGGCGGGAC CTAGTGTTCG GCCAACAGGC AGTTTTCATT TGCACTGCAG TCATCATGCA
    ATCACTCAGA CTCCTGCTTT GCGGCCTGAT CCCCATCATT ACCCCAGTGT TGATCAACCA
    TTACCACCAA ATTGGGAGGC GCGTGTGGAC AGTCACGGTC GTGTTTTTTA CGTGGATCAT
    GTAAACCGGA CAACAACATG GCAGAGACCC ATTCATGCAG CCAAGCGCAC CGGCATGCAG
    AGATCAGGGT CCACACACCA GATGGAGCAG CTCAACCGCA GGTATCAGAA TATTCAGCGG
    ACGATGGCGA GCGAAGACGA GCCTGCAAAC ACTCGCGGAT CTGAGAGGAA CACTGGAGAT
    ACCGAGAGCG AATCCACAAC ACAATCCACA GATCAGAGAA GAGACGGCCC TTCATCTCCG
    GTCAATAACC CGAACGTCTC GCAGCTGCTT CAGTGTCCCG CCGTCAAGTT CATCACTCAC
    CCTGAATTCT TCACTGTCCT CCATGCAAAC TACGGTGCGT ATCGGATGTT CACTAACAGC
    ACCTGCCTGA AGCACATGGT CCTGAAGATC CGCAGAGACG CACGGAACTT TGAGCGATAT
    CAACACAACC GAGACCTCGT CACCTTCATC AACAAGTTCG CTGACACTCA TCTGGAGCTG
    CCGAGGGGCT GGGAGATCAA AACTGACCCG CAGGGCAAGT CCTTCTTCGT GGACCACAAC
    AGTCGAGCGA CGACCTTCAT GGACCCCCGC ATTCCCCTTC AGAACGGCCG CCTGCCGAAT
    CACCTCATAC ACAGACAACA TCTGCAGAGA CTGCGCAGCT ACAGCGCCGG AGAGGCGTCA
    GAAGTGTGTC GCAGTAGAGG AGCTTCTCTG GTCAACAGAA CTGGTCTAGT GTCAGCCGTC
    AGGAGCCAAA ACCAGCCGGA TTCAGTGCCT CACGCCTACA ATGACAAGAT TGTGGCTTTC
    CTCCGTCAAC CAAACATACT GGACACGCTG CAGGAGCGAC AGCCGAGTCT ATCCAGAAAC
    CACACACTCA GGGAGAAGAT CCATTACATC AGGACTGAAG GAGCTCAAGG TGTGGAAAAG
    CTGTCCTGTG ATGCGGATCT GGTGATGCTG CTGAGTCTGT TTGACGAGGA GATCATGTCG
    TTTGTCCCAC CGCACTCCTT CCACCCGGGA CTCAGCTTCT CTCCACGCTG CTCTCCTGGA
    TCCTCACCGC AGAGCTCACC TGGCTTGCAG AGGGCCAGAG CGCCGGCTCC GTACCGCAGA
    GACTTTGAAG CAAAACTGCG CAATTTCTAC AGGAAACTGG AGGCTAAAGG CTATGGACAG
    GGCCCCGGGA AAATTAAGCT GATCGTCAGA AGGGAGCATC TACTAGAGGG CACGTTTAAT
    CAAGTAATGG CGTACTCGCG CAAAGAGCTG CAGAGAAACA AACTCTACAT CACCTTTGTT
    GGAGAGGAAG GTTTGGACTA CAGCGGTCCG TCTCGGGAGT TCTTCTTCTT GTTGTCTCAA
    GAGCTTTTCA ACCCATACTA CGGTCTGTTC GAGTACTCGG CCAACGACAC GTATACCGTC
    CAGATCAGCC CCATGTCTGC GTTTGTGGAG AATCATCTGG AATGGTTTCG TTTCAGTGGC
    CGTATTTTGG GTCTGGCTCT GATCCATCAG TATCTGCTGG ATGCTTTCTT CACTCGGCCG
    TTCTACAAGG CTCTGCTCAG ACTAGTGACG GATTTAAGTG ATCTGGAGTA TCTGGATGAG
    GAGTTTCACC AGAGCTTACA GTGGATGAAG GACAACGATA TCACTGACAT TCTGGATCTC
    ACATTCACAG TCAACGAGGA GGTCTTCGGA CAGGTGACGG AGAGGGAGCT GAAGTCTGGA
    GGCTCCAACA TTCAGGTGAC AGAGAAGAAC AAGAAAGATT ACATCGAGCG AATGGCACGA
    TGGAGGGTGG AGCGGGGGGT CATGCAGCAA GCCGAAGCCC TTGTTAGGGG TTTCTATGAG
    GTGGTGGACT CGCGTCTGGT GTCAGTGTTT GACGCTCGTG AGCTGGAGTT GGTCATTGCA
    GGAACGGCTG AGATTGATTT GAGCGACTGG AGATCCAACA CAGAATATAG AGGAGGTTAT
    CATGATGGCC ACATGGTGAT GCGCTGGTTC TGGGCGGCAG TGGAGCGCTT CAATAATGAG
    CAGCGGCTGC GTCTGCTGCA GTTTGTGACG GGGACCTCCA GTGTTCCCTA TGAGGGATTC
    GCAGCGCTGC GCGGGAGCAA CGGCCTGAGG CGATTCTGCA TTGAGAAGTG GGGAAAAGTC
    ACTTCTCTGC CCAGGGCGCA CACATGCTTC AACCGTCTGG ATCTGCCTCC ATATCCCTCA
    TACACTATGC TGTATGAGAA ACTCCTGACC GCTGTGGAGG AGACCAGCAC CTTTGGTCTG
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325929.1
    CDS264..4949
    Translation

    Target ORF information:

    RefSeq Version XM_021470254.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio E3 ubiquitin-protein ligase HECW1-like (LOC103909734), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470254.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGATCCACC GGCGGATCTT TCTGTCTCAG AACCTGTACC AGAGCAGGTT TGTGAGTCTG 
    GCGGCGATGG CGTCTCCGTC GCGCAGTTCT CCGATGCGCC GGCGCTGGAA AGATGGTTCT
    CGATTCGGTT TCGCTCAGAC AGACCGATCG CTCCATCGCT CAACCAGTGA CACCGACCTG
    CTGACCTTAC AGAGCCGCTC CACACTGACC GTAAGCTCCT CCCAATACTG CATCGGCCAA
    TCAGAGGACA TCTGCATCTG CTGGGACATC AAAGAGGAGG TGGAGCCTGG AGACTGGATC
    GGCATGTACC TCATCGATGA GGTTTTGTCT GAGAATTTCC TGGACTATAA GAGTCGTGGT
    GTGAGCGGAT CCCACAAGGG CCAGATCAGA TGGAGGATCG ACGCCAGCCT CTACTTCATG
    GAGGCTGAGA CGAAGATTTG CTTCAAATAT TATCATGGTT TGAGTGGAGC CCTCAGAGCC
    ACAACACCCA GTGTGACGGT CAGGAACACG TCCGCCTCTG CGCTGAAGCC GGTCATCAGT
    CCGGATCTCC CTCAGTCTCT GGGCAGCAGG AGACTCATCA GCTTCTCCCT CTCCGACGTG
    CAGGCGGTGG GTCTGAAGAA GGGGATGTTC TTTAATCCAG ACCCGTATCT GAAGATCTCC
    ATCCAGCCTG GAAAGCAGAG TCTGTACCCT GCGCTTCCAC ACCACGGCCA GGAGAAACGC
    TCTAGCATCG CCTGCAACAC CATCAGCCCC GTCTGGAAGA GAGAGCGGTT TAACTTTGTG
    TCGCTGCCGA CGGATGTTTT GGAGATCGAG GTTAAAGATA AGTTTGCGAA GAGTCGACCC
    ATTATTAAAC GCTTTCTGGG GAAGCTGGTG GTGCCGGTGC AGAGGCTGCT GGAGAAACAC
    GCCATCGGGG ACCGTGTGGT GAGCTACACT TTAGGTCGGC GGCTTCCAAC AGATCATGTC
    AGCGGCCAGC TGCAGTTTCG CTTCGAGATA ACTTCATCAA TCCATCCAGA TGATGAGGAA
    GTGTCTCTCA ATGCAATGCC AGTCTTTGAT GCGGAGCCAG ACCAGCAGAC AGATCCGGAG
    CCAGTTGAAG ATCCACCACC AGCTTCTGAT CCACCAATGG AGGATGCATT GAGTCTAGAC
    GTTCCCGAGC CTCCACCCGA ATGTCCTCCA GAAATCGAGA TGCGGGATGA TGTTGATGTT
    CAGCTTACAT CTCTATCAGA ACCCAGTAAG GAAACCCAAC CCGAAGCAGA TGAGGAGATG
    GTTACAGCTC AAGGAGATAC ACCAATGAAT GATACAACTC TTGGAGAAAC TGCAGAACAA
    GACACTGGTA TAATGGGGGA TCATGGAGAG ACGCTGGAAG AAACTATGGC TGAGACTCCA
    TCGGAAGTAC TGGACAACCG TGATGCTGAA CATACTGATG GAGTAAATGA AGAGCATGAA
    GTAGAAACAA GCTCTGAACA GGTGGATCTA ATGGTAGACG GAGATAATGA ATGTTCCCCA
    AGGGTACGAG TTCAGTTAAA GCGCAAGAAT CGACCGTGCT CGCTTCCAGT ATCTGAATTA
    GAGACGGTTA TCGCCTCGAC GTGTGAAGAA CCCGAAACGC CGCGCTCTCA TTACATCCGC
    ATTCATCATC TACTCCACAG TCTGCCGTCA GCTCAGTGCC ACCGAGATGA AGAGGAAGAT
    GCACCTGATC CCGAATCTTC AGAAGAAATC ACGATCAAAC CTCAAGAAGA TAAAGAAGAC
    GAAGATGAGG TATCCGAAGC TCAGTCTCCG TCAGTTGTTC CTGAGTGTCC GAGACCGTGT
    TGCAGGCGAA TTTTACCACG TAGCCTCTCA ATCGAGCGTC TTTCTGAACT CAACCAGCTT
    CTGGAGGGAG AAAGACCGTC TCCGTCCACC CTCCGGATAT ACAGTGAAGA CGGGGATTCG
    GGAAGAAGAA GGGAGCCTCG GGAAAGCGAG TGTGAATTTT GTGATAACTC CTGCTATAGT
    ACGTCATGTT ACAGCACGTC CTGTTACAGC ACCTCGTGTT ACAGCACAAA CTCGGGTTAC
    GAGGGCCGAA ACCGGCTGTG CAGCCACACA CGCCTGTCCT CGGTGGACAG CACCCGCCTG
    TCGGAGAGCA CGGTTTTCTC TTCGCAAGAG GAAGAGGAGG AGGAAAACAG CGCGTTTGAG
    TCGGTGTCAG ACTCCATGCG GAGCCCAGAT GTGCGGGAGC CAGGCGGAGG CTCGGTCCAG
    TGGAGGGAGT CTTCTTCAGA AGAGAGTCCT CTGGGGGACG CGGAGGTGGA TGAGTCGCCT
    GTGGCGGGAC CTAGTGTTCG GCCAACAGGC AGTTTTCATT TGCACTGCAG TCATCATGCA
    ATCACTCAGA CTCCTGCTTT GCGGCCTGAT CCCCATCATT ACCCCAGTGT TGATCAACCA
    TTACCACCAA ATTGGGAGGC GCGTGTGGAC AGTCACGGTC GTGTTTTTTA CGTGGATCAT
    GTAAACCGGA CAACAACATG GCAGAGACCC ATTCATGCAG CCAAGCGCAC CGGCATGCAG
    AGATCAGGGT CCACACACCA GATGGAGCAG CTCAACCGCA GGTATCAGAA TATTCAGCGG
    ACGATGGCGA GCGAAGACGA GCCTGCAAAC ACTCGCGGAT CTGAGAGGAA CACTGGAGAT
    ACCGAGAGCG AATCCACAAC ACAATCCACA GATCAGAGAA GAGACGGCCC TTCATCTCCG
    GTCAATAACC CGAACGTCTC GCAGCTGCTT CAGTGTCCCG CCGTCAAGTT CATCACTCAC
    CCTGAATTCT TCACTGTCCT CCATGCAAAC TACGGTGCGT ATCGGATGTT CACTAACAGC
    ACCTGCCTGA AGCACATGGT CCTGAAGATC CGCAGAGACG CACGGAACTT TGAGCGATAT
    CAACACAACC GAGACCTCGT CACCTTCATC AACAAGTTCG CTGACACTCA TCTGGAGCTG
    CCGAGGGGCT GGGAGATCAA AACTGACCCG CAGGGCAAGT CCTTCTTCGT GGACCACAAC
    AGTCGAGCGA CGACCTTCAT GGACCCCCGC ATTCCCCTTC AGAACGGCCG CCTGCCGAAT
    CACCTCATAC ACAGACAACA TCTGCAGAGA CTGCGCAGCT ACAGCGCCGG AGAGGCGTCA
    GAAGTGTGTC GCAGTAGAGG AGCTTCTCTG GTCAACAGAA CTGGTCTAGT GTCAGCCGTC
    AGGAGCCAAA ACCAGCCGGA TTCAGTGCCT CACGCCTACA ATGACAAGAT TGTGGCTTTC
    CTCCGTCAAC CAAACATACT GGACACGCTG CAGGAGCGAC AGCCGAGTCT ATCCAGAAAC
    CACACACTCA GGGAGAAGAT CCATTACATC AGGACTGAAG GAGCTCAAGG TGTGGAAAAG
    CTGTCCTGTG ATGCGGATCT GGTGATGCTG CTGAGTCTGT TTGACGAGGA GATCATGTCG
    TTTGTCCCAC CGCACTCCTT CCACCCGGGA CTCAGCTTCT CTCCACGCTG CTCTCCTGGA
    TCCTCACCGC AGAGCTCACC TGGCTTGCAG AGGGCCAGAG CGCCGGCTCC GTACCGCAGA
    GACTTTGAAG CAAAACTGCG CAATTTCTAC AGGAAACTGG AGGCTAAAGG CTATGGACAG
    GGCCCCGGGA AAATTAAGCT GATCGTCAGA AGGGAGCATC TACTAGAGGG CACGTTTAAT
    CAAGTAATGG CGTACTCGCG CAAAGAGCTG CAGAGAAACA AACTCTACAT CACCTTTGTT
    GGAGAGGAAG GTTTGGACTA CAGCGGTCCG TCTCGGGAGT TCTTCTTCTT GTTGTCTCAA
    GAGCTTTTCA ACCCATACTA CGGTCTGTTC GAGTACTCGG CCAACGACAC GTATACCGTC
    CAGATCAGCC CCATGTCTGC GTTTGTGGAG AATCATCTGG AATGGTTTCG TTTCAGTGGC
    CGTATTTTGG GTCTGGCTCT GATCCATCAG TATCTGCTGG ATGCTTTCTT CACTCGGCCG
    TTCTACAAGG CTCTGCTCAG ACTAGTGACG GATTTAAGTG ATCTGGAGTA TCTGGATGAG
    GAGTTTCACC AGAGCTTACA GTGGATGAAG GACAACGATA TCACTGACAT TCTGGATCTC
    ACATTCACAG TCAACGAGGA GGTCTTCGGA CAGGTGACGG AGAGGGAGCT GAAGTCTGGA
    GGCTCCAACA TTCAGGTGAC AGAGAAGAAC AAGAAAGATT ACATCGAGCG AATGGCACGA
    TGGAGGGTGG AGCGGGGGGT CATGCAGCAA GCCGAAGCCC TTGTTAGGGG TTTCTATGAG
    GTGGTGGACT CGCGTCTGGT GTCAGTGTTT GACGCTCGTG AGCTGGAGTT GGTCATTGCA
    GGAACGGCTG AGATTGATTT GAGCGACTGG AGATCCAACA CAGAATATAG AGGAGGTTAT
    CATGATGGCC ACATGGTGAT GCGCTGGTTC TGGGCGGCAG TGGAGCGCTT CAATAATGAG
    CAGCGGCTGC GTCTGCTGCA GTTTGTGACG GGGACCTCCA GTGTTCCCTA TGAGGGATTC
    GCAGCGCTGC GCGGGAGCAA CGGCCTGAGG CGATTCTGCA TTGAGAAGTG GGGAAAAGTC
    ACTTCTCTGC CCAGGGCGCA CACATGCTTC AACCGTCTGG ATCTGCCTCC ATATCCCTCA
    TACACTATGC TGTATGAGAA ACTCCTGACC GCTGTGGAGG AGACCAGCAC CTTTGGTCTG
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.