si:ch73-281f12.4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	si:ch73-281f12.4
Entrez Gene ID	102216267
Full Name	si:ch73-281f12.4
Synonyms	zmp:0000000679
General protein information	Preferred Names si:ch73-281f12.4 Names si:ch73-281f12.4 Ras-associated forkhead-associated dilute domain protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001285388.2	NP_001272317.2	si:ch73-281f12.4
NM_001285388.1	NP_001272317.1	si:ch73-281f12.4
XM_021469262.1	XP_021324937.1	si:ch73-281f12.4 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following si:ch73-281f12.4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the si:ch73-281f12.4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa98451	NM_001285388.2 Latest version!	Danio rerio si:ch73-281f12.4 (si:ch73-281f12.4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa141540	XM_021469262.1 Latest version!	Danio rerio si:ch73-281f12.4 (si:ch73-281f12.4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa01836	NM_001285388.1 Latest version!	Danio rerio si:ch73-281f12.4 (si:ch73-281f12.4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

si:ch73-281f12.4 ( NM_001285388.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001272317.2 Danio rerio si:ch73-281f12.4 (si:ch73-281f12.4), mRNA.

Price & Availability↑

CloneID	ODa98451
Clone ID Related Accession (Same CDS sequence)	NM_001285388.2
Accession Version	NM_001285388.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	si:ch73-281f12.4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU651584.9 and CU570691.15. On Mar 9, 2017 this sequence version replaced NM_001285388.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01020808.1, JF519851.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGATTTCGG AGGAAAGGAG CAATCACGTC TCTAAACAGG CTCTGGGCTT TCCTGTCGGA 
CTGCTGATCC GCTCGCCCAA GCGAAAACTA GCGATGCTGG GCAGAAAGGC GAGCAATGGA 
TCTCTACAGT CCAGCACGTC CGACAGCACG TCTCAGTCTG TGGAGAGCAC AGGAGTCCGG 
CAGCCGGCCC GCAGCAAAAT ACGCCGCCAC AACAACCGGC TAACCACCGT CTTCAACCGA 
AGCCCCGTCC TGCAGAGCGG ACGGCAGCGG CAGGATGCCC GGAGCCTGGA CCTGCAGATG 
GAGGAGGACG ACCCTGCAGA GCTGTCCAGC CAGAGGACAG TCCCGGGCAT CCTGAAGATA 
TTCGGCAGTG ACATCTGCCA GGGGACCAAC TATAAGAGTG TGCTGGCCAC CAGTAACTCC 
AGTGCCAACC AGCTGGTCAA AGAGGCTCTG GAGAGGTACT GCTTGGAAAA GGAAGACCCC 
AGCGCTTATG TGCTCTGCGA TGTGATTGGA CGAACTGGAT CCGATCACGA GTGGAAGACT 
GAGTGCTTTC GTGTTGTCGG TGATTATGAG AAGCCGCTCA TGTTGCAGTC ACTCTGGAAA 
CCCAAGGAGG GATTCTCCAG ACGCTTCGAG ATCCAGAAGA GAGCAACTGT AGAGGCGCAA 
AGTGCCAAGG ATACAGACAC CATCACTGCA GGACTCAATG CCCAGGCCCG AAAGCTGCAG 
CATGCCCGTT CACGGGTCAC CTCGCTGTTT GTGGACGGGA CGCACGAGGG AGTGGACGCT 
CTGGGCATCT GGAGGAGCTT AAGTGACATG GACGTCTCCA CTATGGGGAA AGAAGCTAGC 
GTGAGCCGTC CTGCAGCCCC GCGGGATGAT CCCGAAGCAG AGGCGGATAA AGAGGTGCAG 
CGTCTCAATG CGGAGAAAGA GGAGACGGAA AGCAGCGACG ACAACCACAC GCAGTACTCC 
ATCCACCTGC CCTTTGATTT CCCATATTTC CTCCTCCTGC AAGGATACAG CCACAGACAG 
GATTTTCTGA TCTATCTGAT GAGCGGGAAC ACGTGTGTGT TCGGCAGCTC CTCTGAATCC 
TCCACCGGAG TGAACCAGGA GTCACTAAAG GTGGACATTT TACTCTTCGC TCCTGATGTT 
TCACCTCAGC ACTGCTGCGT TCAGCGACAG GATGCCACCG CTGACCAAAC TAAGATGCAA 
ACCAAACTCA GACCACTGCA CAGTGCACAC GTTACGCACA ACGGCGTCCC GCTCCAGGAC 
GAAATCGAGT TGTGTCCAGG CGACCTGATA GGCCTCGGTC AGCACTATCT CTTCATGTTC 
AAAGACCCGA CGGCCACGGG AGCCTTTCAG ACTCCGTCCT GGATGGCTAC ATTGTGTCCC 
CCAGCCACGA CGTCTCCCTG TAAACTGTGT GGGACATCAG TAAGGAGGAG GAGGACTCAA 
AAAATAGCCG CTCGCTGGAG GGATTCTGAT GGTAGGGTGG TTTCGCTGTC CTATCAAATG 
GAGCAGGAGG ATCGGGTGTT GGAGAAGATC GTCAGTATGG TGGATGCAGG AGGAGAAGAT 
CCCAAACTGA CCGCAGCCTT TCTGCTGTGT CTGTGCATCC AACAATCTGC CACTGACTTT 
GAGCTGCTTC ATCTGCGGAG ACTGCTGCTC TCCATTGCCA ATCACATACA GCTCGACATG 
TGGGAAAGAA CAAAGGAACT TGCAGCTCTG CATCCAGACA CTAACTCCAT TGATGGGACA 
GCGGAGCAGC CTGTGCTGAA CATGAAGGAA CTGATCCCAG GCCTGCAGCC CCTTGTGCTG 
TGGATGTCCA ACTCCATTGA GCTGCTGCAC TTCATACAAC ATGAGGTCCC TCTGCTACTA 
ACATGGAGAC AGCAGGACAG ACAGGAACAG GACAAAGAAT GGCTGGATGG TCAGATCCAG 
TGCACTCGTG CTGCCAGTGA GGAGGCCATG ACGGTCCTGG AGGAGGTCAT CATGTTCACC 
TTCCAGCAGT GCGTCTATTA TCTTACCAAA ATTCTGTATG CAGTGTTGTC GGGGCTGCTG 
GACAGTAACC CTTTCTCCGA GAGCGGTCAG CTGCGCATGC CTGCGGGAGT CAGTCAGATC 
CTGGACGTCC TGAAGGAGGC TCTGCATCTG CTTAATACTT TCCAGGTGCA TCGAGAGATC 
ACGTCTCAAC TGCTGACCTA CCTGTTCTTC TTCACCAACG CCTCGCTCTT CAACACACTC 
ATGGAAAGAG GGTCTGGAGG TGGCTTCTAC CAGTGGTCCA GAGGAGTTCA GATCCGAGCC 
AACCTGGACT TACTGATGGA CTGGATTCAG AGCATTGGTC TTGGAGATTT GGCTGCTGAG 
TTCTTCCAGA AGCTCTCTTC AGCTGTGAAC CTGCTGGCAA CGCCCAAAGA GACTCTACTG 
CAGGCCTCGT GGTCCACCCT GAGAGCTGAG TTTGTTCATC TGAACCCTGC GCAGCTTCAT 
CACATGCTGA GAGAGTACAA CACATCTCGC ACATGTCCTC CATGCTGGAC TCCGTCTCCA 
GAGGACGCAG CGGCGGCGCT CCATACCTCT AATATCCTGG AAAGTTTCGA CAACCATCCT 
CCTCTAATCC TGCCCAGCAA CATTTTTCAT CTGGAGCTGG GAAAACCCAT CGCAGAGCCA 
GGGCTGTTCA CTCCTCTCCA GCGTCTGCAG GAGTTTATCC AGACCCTGTC GGACTGCCAG 
ACACAGCCTG ACCATCAGCC CGCACCGGAC TCGTCCAGTA ACCTGACTGA AGAGTCGACA 
TCTAAATCAG TCACCATCCA GAATCACGAA TCAAATCATC AGCATCACGT CTCAAGAACA 
CCTCCTCAGT TTAGGGCGTC TCACAATAAC CTGAGTTCCT GTGAGGCCGT CCTGACCCAG 
AAACTCAAGT CTCTGGAGCT TCAGAACTGC TTGTCTGGGA ACGCAGACCT GGTCTTCCAC 
AAGAGTCTGG CCCTGGACCC ATCCTGCCTG CTGACGCCCC CTAACACCCC ACAGAACCTG 
GACCAGGCCG AGCTGGAGGC CAGTCTGCAG GACGGGGGAC AGCAGAGCAT ACGCCAGCAC 
CACAGAGTAG AGAACGGCCT CTCAGAAAAC ACAGCTCAAA GAAAAGACGA GGATGAGGAT 
GACAGAGATG AAGTCTTCAC TGTTGAGCTG AAGAAAGGCC CTCATGGTCT CGGGCTGGCG 
TTGGTGGATG GACAGAAAAC TGCTCTGCGG GTGAATGGGG TCTATGTGAA GTCAGTGGTG 
CCCGATTCTC CAGCGGCCCA GAGCCAGAGC CTGAGAACAG GAGACCGGAT CCTCGCCGTC 
AACGGAGTCA GTCTGGTGGG AATGGACTAC CACAGTGGAC GAGAGCTCAT CCGCACATCA 
GGAGATTCAC TCCGCTTACT GGTGGCCAAA ACTGAGTCCA TGAGCAGCGC CTGGAGCTGC 
ATCACCAGAT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001272317.2
CDS	1..3435
Translation	MISEERSNHVSKQALGFPVGLLIRSPKRKLAMLGRKASNGSLQSSTSDSTSQSVESTGVRQPARSKIRRHNNRLTTVFNRSPVLQSGRQRQDARSLDLQMEEDDPAELSSQRTVPGILKIFGSDICQGTNYKSVLATSNSSANQLVKEALERYCLEKEDPSAYVLCDVIGRTGSDHEWKTECFRVVGDYEKPLMLQSLWKPKEGFSRRFEIQKRATVEAQSAKDTDTITAGLNAQARKLQHARSRVTSLFVDGTHEGVDALGIWRSLSDMDVSTMGKEASVSRPAAPRDDPEAEADKEVQRLNAEKEETESSDDNHTQYSIHLPFDFPYFLLLQGYSHRQDFLIYLMSGNTCVFGSSSESSTGVNQESLKVDILLFAPDVSPQHCCVQRQDATADQTKMQTKLRPLHSAHVTHNGVPLQDEIELCPGDLIGLGQHYLFMFKDPTATGAFQTPSWMATLCPPATTSPCKLCGTSVRRRRTQKIAARWRDSDGRVVSLSYQMEQEDRVLEKIVSMVDAGGEDPKLTAAFLLCLCIQQSATDFELLHLRRLLLSIANHIQLDMWERTKELAALHPDTNSIDGTAEQPVLNMKELIPGLQPLVLWMSNSIELLHFIQHEVPLLLTWRQQDRQEQDKEWLDGQIQCTRAASEEAMTVLEEVIMFTFQQCVYYLTKILYAVLSGLLDSNPFSESGQLRMPAGVSQILDVLKEALHLLNTFQVHREITSQLLTYLFFFTNASLFNTLMERGSGGGFYQWSRGVQIRANLDLLMDWIQSIGLGDLAAEFFQKLSSAVNLLATPKETLLQASWSTLRAEFVHLNPAQLHHMLREYNTSRTCPPCWTPSPEDAAAALHTSNILESFDNHPPLILPSNIFHLELGKPIAEPGLFTPLQRLQEFIQTLSDCQTQPDHQPAPDSSSNLTEESTSKSVTIQNHESNHQHHVSRTPPQFRASHNNLSSCEAVLTQKLKSLELQNCLSGNADLVFHKSLALDPSCLLTPPNTPQNLDQAELEASLQDGGQQSIRQHHRVENGLSENTAQRKDEDEDDRDEVFTVELKKGPHGLGLALVDGQKTALRVNGVYVKSVVPDSPAAQSQSLRTGDRILAVNGVSLVGMDYHSGRELIRTSGDSLRLLVAKTESMSSAWSCITRC

Target ORF information:

RefSeq Version	NM_001285388.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch73-281f12.4 (si:ch73-281f12.4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001285388.2

ORF Insert Sequence:

ATGATTTCGG AGGAAAGGAG CAATCACGTC TCTAAACAGG CTCTGGGCTT TCCTGTCGGA 
CTGCTGATCC GCTCGCCCAA GCGAAAACTA GCGATGCTGG GCAGAAAGGC GAGCAATGGA 
TCTCTACAGT CCAGCACGTC CGACAGCACG TCTCAGTCTG TGGAGAGCAC AGGAGTCCGG 
CAGCCGGCCC GCAGCAAAAT ACGCCGCCAC AACAACCGGC TAACCACCGT CTTCAACCGA 
AGCCCCGTCC TGCAGAGCGG ACGGCAGCGG CAGGATGCCC GGAGCCTGGA CCTGCAGATG 
GAGGAGGACG ACCCTGCAGA GCTGTCCAGC CAGAGGACAG TCCCGGGCAT CCTGAAGATA 
TTCGGCAGTG ACATCTGCCA GGGGACCAAC TATAAGAGTG TGCTGGCCAC CAGTAACTCC 
AGTGCCAACC AGCTGGTCAA AGAGGCTCTG GAGAGGTACT GCTTGGAAAA GGAAGACCCC 
AGCGCTTATG TGCTCTGCGA TGTGATTGGA CGAACTGGAT CCGATCACGA GTGGAAGACT 
GAGTGCTTTC GTGTTGTCGG TGATTATGAG AAGCCGCTCA TGTTGCAGTC ACTCTGGAAA 
CCCAAGGAGG GATTCTCCAG ACGCTTCGAG ATCCAGAAGA GAGCAACTGT AGAGGCGCAA 
AGTGCCAAGG ATACAGACAC CATCACTGCA GGACTCAATG CCCAGGCCCG AAAGCTGCAG 
CATGCCCGTT CACGGGTCAC CTCGCTGTTT GTGGACGGGA CGCACGAGGG AGTGGACGCT 
CTGGGCATCT GGAGGAGCTT AAGTGACATG GACGTCTCCA CTATGGGGAA AGAAGCTAGC 
GTGAGCCGTC CTGCAGCCCC GCGGGATGAT CCCGAAGCAG AGGCGGATAA AGAGGTGCAG 
CGTCTCAATG CGGAGAAAGA GGAGACGGAA AGCAGCGACG ACAACCACAC GCAGTACTCC 
ATCCACCTGC CCTTTGATTT CCCATATTTC CTCCTCCTGC AAGGATACAG CCACAGACAG 
GATTTTCTGA TCTATCTGAT GAGCGGGAAC ACGTGTGTGT TCGGCAGCTC CTCTGAATCC 
TCCACCGGAG TGAACCAGGA GTCACTAAAG GTGGACATTT TACTCTTCGC TCCTGATGTT 
TCACCTCAGC ACTGCTGCGT TCAGCGACAG GATGCCACCG CTGACCAAAC TAAGATGCAA 
ACCAAACTCA GACCACTGCA CAGTGCACAC GTTACGCACA ACGGCGTCCC GCTCCAGGAC 
GAAATCGAGT TGTGTCCAGG CGACCTGATA GGCCTCGGTC AGCACTATCT CTTCATGTTC 
AAAGACCCGA CGGCCACGGG AGCCTTTCAG ACTCCGTCCT GGATGGCTAC ATTGTGTCCC 
CCAGCCACGA CGTCTCCCTG TAAACTGTGT GGGACATCAG TAAGGAGGAG GAGGACTCAA 
AAAATAGCCG CTCGCTGGAG GGATTCTGAT GGTAGGGTGG TTTCGCTGTC CTATCAAATG 
GAGCAGGAGG ATCGGGTGTT GGAGAAGATC GTCAGTATGG TGGATGCAGG AGGAGAAGAT 
CCCAAACTGA CCGCAGCCTT TCTGCTGTGT CTGTGCATCC AACAATCTGC CACTGACTTT 
GAGCTGCTTC ATCTGCGGAG ACTGCTGCTC TCCATTGCCA ATCACATACA GCTCGACATG 
TGGGAAAGAA CAAAGGAACT TGCAGCTCTG CATCCAGACA CTAACTCCAT TGATGGGACA 
GCGGAGCAGC CTGTGCTGAA CATGAAGGAA CTGATCCCAG GCCTGCAGCC CCTTGTGCTG 
TGGATGTCCA ACTCCATTGA GCTGCTGCAC TTCATACAAC ATGAGGTCCC TCTGCTACTA 
ACATGGAGAC AGCAGGACAG ACAGGAACAG GACAAAGAAT GGCTGGATGG TCAGATCCAG 
TGCACTCGTG CTGCCAGTGA GGAGGCCATG ACGGTCCTGG AGGAGGTCAT CATGTTCACC 
TTCCAGCAGT GCGTCTATTA TCTTACCAAA ATTCTGTATG CAGTGTTGTC GGGGCTGCTG 
GACAGTAACC CTTTCTCCGA GAGCGGTCAG CTGCGCATGC CTGCGGGAGT CAGTCAGATC 
CTGGACGTCC TGAAGGAGGC TCTGCATCTG CTTAATACTT TCCAGGTGCA TCGAGAGATC 
ACGTCTCAAC TGCTGACCTA CCTGTTCTTC TTCACCAACG CCTCGCTCTT CAACACACTC 
ATGGAAAGAG GGTCTGGAGG TGGCTTCTAC CAGTGGTCCA GAGGAGTTCA GATCCGAGCC 
AACCTGGACT TACTGATGGA CTGGATTCAG AGCATTGGTC TTGGAGATTT GGCTGCTGAG 
TTCTTCCAGA AGCTCTCTTC AGCTGTGAAC CTGCTGGCAA CGCCCAAAGA GACTCTACTG 
CAGGCCTCGT GGTCCACCCT GAGAGCTGAG TTTGTTCATC TGAACCCTGC GCAGCTTCAT 
CACATGCTGA GAGAGTACAA CACATCTCGC ACATGTCCTC CATGCTGGAC TCCGTCTCCA 
GAGGACGCAG CGGCGGCGCT CCATACCTCT AATATCCTGG AAAGTTTCGA CAACCATCCT 
CCTCTAATCC TGCCCAGCAA CATTTTTCAT CTGGAGCTGG GAAAACCCAT CGCAGAGCCA 
GGGCTGTTCA CTCCTCTCCA GCGTCTGCAG GAGTTTATCC AGACCCTGTC GGACTGCCAG 
ACACAGCCTG ACCATCAGCC CGCACCGGAC TCGTCCAGTA ACCTGACTGA AGAGTCGACA 
TCTAAATCAG TCACCATCCA GAATCACGAA TCAAATCATC AGCATCACGT CTCAAGAACA 
CCTCCTCAGT TTAGGGCGTC TCACAATAAC CTGAGTTCCT GTGAGGCCGT CCTGACCCAG 
AAACTCAAGT CTCTGGAGCT TCAGAACTGC TTGTCTGGGA ACGCAGACCT GGTCTTCCAC 
AAGAGTCTGG CCCTGGACCC ATCCTGCCTG CTGACGCCCC CTAACACCCC ACAGAACCTG 
GACCAGGCCG AGCTGGAGGC CAGTCTGCAG GACGGGGGAC AGCAGAGCAT ACGCCAGCAC 
CACAGAGTAG AGAACGGCCT CTCAGAAAAC ACAGCTCAAA GAAAAGACGA GGATGAGGAT 
GACAGAGATG AAGTCTTCAC TGTTGAGCTG AAGAAAGGCC CTCATGGTCT CGGGCTGGCG 
TTGGTGGATG GACAGAAAAC TGCTCTGCGG GTGAATGGGG TCTATGTGAA GTCAGTGGTG 
CCCGATTCTC CAGCGGCCCA GAGCCAGAGC CTGAGAACAG GAGACCGGAT CCTCGCCGTC 
AACGGAGTCA GTCTGGTGGG AATGGACTAC CACAGTGGAC GAGAGCTCAT CCGCACATCA 
GGAGATTCAC TCCGCTTACT GGTGGCCAAA ACTGAGTCCA TGAGCAGCGC CTGGAGCTGC 
ATCACCAGAT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch73-281f12.4 ( XM_021469262.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324937.1 Danio rerio si:ch73-281f12.4 (si:ch73-281f12.4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa141540
Clone ID Related Accession (Same CDS sequence)	XM_021469262.1
Accession Version	XM_021469262.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	si:ch73-281f12.4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTCGG AGGAAAGGAG CAATCACGTC TCTAAACAGG CTCTGGGCTT TCCTGTCGGA 
CTGCTGATCC GCTCGCCCAA GCGAAAACTA GCGATGCTGG GCAGAAAGGC GAGCAATGGA 
TCTCTACAGT CCAGCACGTC CGACAGCACG TCTCAGTCTG TGGAGAGCAC AGGAGTCCGG 
CAGCCGGCCC GCAGCAAAAT ACGCCGCCAC AACAACCGGC TAACCACCGT CTTCAACCGA 
AGCCCCGTCC TGCAGAGCGG ACGGCAGCGG CAGGATGCCC GGAGCCTGGA CCTGCAGATG 
GAGGAGGACG ACCCTGCAGA GCTGTCCAGC CAGAGGACAG TCCCGGGCAT CCTGAAGATA 
TTCGGCAGTG ACATCTGCCA GGGGACCAAC TATAAGAGTG TGCTGGCCAC CAGTAACTCC 
AGTGCCAACC AGCTGGTCAA AGAGGCTCTG GAGAGGTACT GCTTGGAAAA GGAAGACCCC 
AGCGCTTATG TGCTCTGCGA TGTGATTGGA CGAACTGGAT CCGATCACGA GTGGAAGACT 
GAGTGCTTTC GTGTTGTCGG TGATTATGAG AAGCCGCTCA TGTTGCAGTC ACTCTGGAAA 
CCCAAGGAGG GATTCTCCAG ACGCTTCGAG ATCCAGAAGA GAGCAACTGT AGAGGCGCAA 
AGTGCCAAGG ATACAGACAC CATCACTGCA GGACTCAATG CCCAGGCCCG AAAGCTGCAG 
CATGCCCGTT CACGGGTCAC CTCGCTGTTT GTGGACGGGA CGCACGAGGG AGTGGACGCT 
CTGGGCATCT GGAGGAGCTT AAGTGACATG GACGTCTCCA CTATGGGGAA AGAAGCTAGC 
GTGAGCCGTC CTGCAGCCCC GCGGGATGAT CCCGAAGCAG AGGCGGATAA AGAGGTGCAG 
CGTCTCAATG CGGAGAAAGA GGAGACGGAA AGCAGCGACG ACAACCACAC GCAGTACTCC 
ATCCACCTGC CCTTTGATTT CCCATATTTC CTCCTCCTGC AAGGATACAG CCACAGACAG 
GATTTTCTGA TCTATCTGAT GAGCGGGAAC ACGTGTGTGT TCGGCAGCTC CTCTGAATCC 
TCCACCGGAG TGAACCAGGA GTCACTAAAG GTGGACATTT TACTCTTCGC TCCTGATGTT 
TCACCTCAGC ACTGCTGCGT TCAGCGACAG GATGCCACCG CTGACCAAAC TAAGATGCAA 
ACCAAACTCA GACCACTGCA CAGTGCACAC GTTACGCACA ACGGCGTCCC GCTCCAGGAC 
GAAATCGAGT TGTGTCCAGG CGACCTGATA GGCCTCGGTC AGCACTATCT CTTCATGTTC 
AAAGACCCGA CGGCCACGGG AGCCTTTCAG ACTCCGTCCT GGATGGCTAC ATTGTGTCCC 
CCAGCCACGA CGTCTCCCTG TAAACTGTGT GGGACATCAG TAAGGAGGAG GAGGACTCAA 
AAAATAGCCG CTCGCTGGAG GGATTCTGAT GGTAGGGTGG TTTCGCTGTC CTATCAAATG 
GAGCAGGAGG ATCGGGTGTT GGAGAAGATC GTCAGTATGG TGGATGCAGG AGGAGAAGAT 
CCCAAACTGA CCGCAGCCTT TCTGCTGTGT CTGTGCATCC AACAATCTGC CACTGACTTT 
GAGCTGCTTC ATCTGCGGAG ACTGCTGCTC TCCATTGCCA ATCACATACA GCTCGACATG 
TGGGAAAGAA CAAAGGAACT TGCAGCTCTG CATCCAGACA CTAACTCCAT TGATGGGACA 
GCGGAGCAGC CTGTGCTGAA CATGAAGGAA CTGATCCCAG GCCTGCAGCC CCTTGTGCTG 
TGGATGTCCA ACTCCATTGA GCTGCTGCAC TTCATACAAC ATGAGGTCCC TCTGCTACTA 
ACATGGAGAC AGCAGGACAG ACAGGAACAG GACAAAGAAT GGCTGGATGG TCAGATCCAG 
TGCACTCGTG CTGCCAGTGA GGAGGCCATG ACGGTCCTGG AGGAGGTCAT CATGTTCACC 
TTCCAGCAGT GCGTCTATTA TCTTACCAAA ATTCTGTATG CAGTGTTGTC GGGGCTGCTG 
GACAGTAACC CTTTCTCCGA GAGCGGTCAG CTGCGCATGC CTGCGGGAGT CAGTCAGATC 
CTGGACGTCC TGAAGGAGGC TCTGCATCTG CTTAATACTT TCCAGGTGCA TCGAGAGATC 
ACGTCTCAAC TGCTGACCTA CCTGTTCTTC TTCACCAACG CCTCGCTCTT CAACACACTC 
ATGGAAAGAG GGTCTGGAGG TGGCTTCTAC CAGTGGTCCA GAGGAGTTCA GATCCGAGCC 
AACCTGGACT TACTGATGGA CTGGATTCAG AGCATTGGTC TTGGAGATTT GGCTGCTGAG 
TTCTTCCAGA AGCTCTCTTC AGCTGTGAAC CTGCTGGCAA CGCCCAAAGA GACTCTACTG 
CAGGCCTCGT GGTCCACCCT GAGAGCTGAG TTTGTTCATC TGAACCCTGC GCAGCTTCAT 
CACATGCTGA GAGAGTACAA CACATCTCGC ACATGTCCTC CATGCTGGAC TCCGTCTCCA 
GAGGACGCAG CGGCGGCGCT CCATACCTCT AATATCCTGG AAAGTTTCGA CAACCATCCT 
CCTCTAATCC TGCCCAGCAA CATTTTTCAT CTGGAGCTGG GAAAACCCAT CGCAGAGCCA 
GGGCTGTTCA CTCCTCTCCA GCGTCTGCAG GAGTTTATCC AGACCCTGTC GGACTGCCAG 
ACACAGCCTG ACCATCAGCC CGCACCGGAC TCGTCCAGTA ACCTGACTGA AGAGTCGACA 
TCTAAATCAG TCACCATCCA GAATCACGAA TCAAATCATC AGCATCACGT CTCAAGAACA 
CCTCCTCAGT TTAGGGCGTC TCACAATAAC CTGAGTTCCT GTGAGGCCGT CCTGACCCAG 
AAACTCAAGT CTCTGGAGCT TCAGAACTGC TTGTCTGGGA ACGCAGACCT GGTCTTCCAC 
AAGAGTCTGG CCCTGGACCC ATCCTGCCTG CTGACGCCCC CTAACACCCC ACAGAACCTG 
GACCAGGCCG AGCTGGAGGC CAGTCTGCAG GACGGGGGAC AGCAGAGCAT ACGCCAGCAC 
CACAGAGAGA ACGGCCTCTC AGAAAACACA GCTCAAAGAA AAGACGAGGA TGAGGATGAC 
AGAGATGAAG TCTTCACTGT TGAGCTGAAG AAAGGCCCTC ATGGTCTCGG GCTGGCGTTG 
GTGGATGGAC AGAAAACTGC TCTGCGGGTG AATGGGGTCT ATGTGAAGTC AGTGGTGCCC 
GATTCTCCAG CGGCCCAGAG CCAGAGCCTG AGAACAGGAG ACCGGATCCT CGCCGTCAAC 
GGAGTCAGTC TGGTGGGAAT GGACTACCAC AGTGGACGAG AGCTCATCCG CACATCAGGA 
GATTCACTCC GCTTACTGGT GGCCAAAACT GAGTCCATGA GCAGCGCCTG GAGCTGCATC 
ACCAGATGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324937.1
CDS	438..3869
Translation	MISEERSNHVSKQALGFPVGLLIRSPKRKLAMLGRKASNGSLQSSTSDSTSQSVESTGVRQPARSKIRRHNNRLTTVFNRSPVLQSGRQRQDARSLDLQMEEDDPAELSSQRTVPGILKIFGSDICQGTNYKSVLATSNSSANQLVKEALERYCLEKEDPSAYVLCDVIGRTGSDHEWKTECFRVVGDYEKPLMLQSLWKPKEGFSRRFEIQKRATVEAQSAKDTDTITAGLNAQARKLQHARSRVTSLFVDGTHEGVDALGIWRSLSDMDVSTMGKEASVSRPAAPRDDPEAEADKEVQRLNAEKEETESSDDNHTQYSIHLPFDFPYFLLLQGYSHRQDFLIYLMSGNTCVFGSSSESSTGVNQESLKVDILLFAPDVSPQHCCVQRQDATADQTKMQTKLRPLHSAHVTHNGVPLQDEIELCPGDLIGLGQHYLFMFKDPTATGAFQTPSWMATLCPPATTSPCKLCGTSVRRRRTQKIAARWRDSDGRVVSLSYQMEQEDRVLEKIVSMVDAGGEDPKLTAAFLLCLCIQQSATDFELLHLRRLLLSIANHIQLDMWERTKELAALHPDTNSIDGTAEQPVLNMKELIPGLQPLVLWMSNSIELLHFIQHEVPLLLTWRQQDRQEQDKEWLDGQIQCTRAASEEAMTVLEEVIMFTFQQCVYYLTKILYAVLSGLLDSNPFSESGQLRMPAGVSQILDVLKEALHLLNTFQVHREITSQLLTYLFFFTNASLFNTLMERGSGGGFYQWSRGVQIRANLDLLMDWIQSIGLGDLAAEFFQKLSSAVNLLATPKETLLQASWSTLRAEFVHLNPAQLHHMLREYNTSRTCPPCWTPSPEDAAAALHTSNILESFDNHPPLILPSNIFHLELGKPIAEPGLFTPLQRLQEFIQTLSDCQTQPDHQPAPDSSSNLTEESTSKSVTIQNHESNHQHHVSRTPPQFRASHNNLSSCEAVLTQKLKSLELQNCLSGNADLVFHKSLALDPSCLLTPPNTPQNLDQAELEASLQDGGQQSIRQHHRENGLSENTAQRKDEDEDDRDEVFTVELKKGPHGLGLALVDGQKTALRVNGVYVKSVVPDSPAAQSQSLRTGDRILAVNGVSLVGMDYHSGRELIRTSGDSLRLLVAKTESMSSAWSCITRC

Target ORF information:

RefSeq Version	XM_021469262.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch73-281f12.4 (si:ch73-281f12.4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469262.1

ORF Insert Sequence:

ATGATTTCGG AGGAAAGGAG CAATCACGTC TCTAAACAGG CTCTGGGCTT TCCTGTCGGA 
CTGCTGATCC GCTCGCCCAA GCGAAAACTA GCGATGCTGG GCAGAAAGGC GAGCAATGGA 
TCTCTACAGT CCAGCACGTC CGACAGCACG TCTCAGTCTG TGGAGAGCAC AGGAGTCCGG 
CAGCCGGCCC GCAGCAAAAT ACGCCGCCAC AACAACCGGC TAACCACCGT CTTCAACCGA 
AGCCCCGTCC TGCAGAGCGG ACGGCAGCGG CAGGATGCCC GGAGCCTGGA CCTGCAGATG 
GAGGAGGACG ACCCTGCAGA GCTGTCCAGC CAGAGGACAG TCCCGGGCAT CCTGAAGATA 
TTCGGCAGTG ACATCTGCCA GGGGACCAAC TATAAGAGTG TGCTGGCCAC CAGTAACTCC 
AGTGCCAACC AGCTGGTCAA AGAGGCTCTG GAGAGGTACT GCTTGGAAAA GGAAGACCCC 
AGCGCTTATG TGCTCTGCGA TGTGATTGGA CGAACTGGAT CCGATCACGA GTGGAAGACT 
GAGTGCTTTC GTGTTGTCGG TGATTATGAG AAGCCGCTCA TGTTGCAGTC ACTCTGGAAA 
CCCAAGGAGG GATTCTCCAG ACGCTTCGAG ATCCAGAAGA GAGCAACTGT AGAGGCGCAA 
AGTGCCAAGG ATACAGACAC CATCACTGCA GGACTCAATG CCCAGGCCCG AAAGCTGCAG 
CATGCCCGTT CACGGGTCAC CTCGCTGTTT GTGGACGGGA CGCACGAGGG AGTGGACGCT 
CTGGGCATCT GGAGGAGCTT AAGTGACATG GACGTCTCCA CTATGGGGAA AGAAGCTAGC 
GTGAGCCGTC CTGCAGCCCC GCGGGATGAT CCCGAAGCAG AGGCGGATAA AGAGGTGCAG 
CGTCTCAATG CGGAGAAAGA GGAGACGGAA AGCAGCGACG ACAACCACAC GCAGTACTCC 
ATCCACCTGC CCTTTGATTT CCCATATTTC CTCCTCCTGC AAGGATACAG CCACAGACAG 
GATTTTCTGA TCTATCTGAT GAGCGGGAAC ACGTGTGTGT TCGGCAGCTC CTCTGAATCC 
TCCACCGGAG TGAACCAGGA GTCACTAAAG GTGGACATTT TACTCTTCGC TCCTGATGTT 
TCACCTCAGC ACTGCTGCGT TCAGCGACAG GATGCCACCG CTGACCAAAC TAAGATGCAA 
ACCAAACTCA GACCACTGCA CAGTGCACAC GTTACGCACA ACGGCGTCCC GCTCCAGGAC 
GAAATCGAGT TGTGTCCAGG CGACCTGATA GGCCTCGGTC AGCACTATCT CTTCATGTTC 
AAAGACCCGA CGGCCACGGG AGCCTTTCAG ACTCCGTCCT GGATGGCTAC ATTGTGTCCC 
CCAGCCACGA CGTCTCCCTG TAAACTGTGT GGGACATCAG TAAGGAGGAG GAGGACTCAA 
AAAATAGCCG CTCGCTGGAG GGATTCTGAT GGTAGGGTGG TTTCGCTGTC CTATCAAATG 
GAGCAGGAGG ATCGGGTGTT GGAGAAGATC GTCAGTATGG TGGATGCAGG AGGAGAAGAT 
CCCAAACTGA CCGCAGCCTT TCTGCTGTGT CTGTGCATCC AACAATCTGC CACTGACTTT 
GAGCTGCTTC ATCTGCGGAG ACTGCTGCTC TCCATTGCCA ATCACATACA GCTCGACATG 
TGGGAAAGAA CAAAGGAACT TGCAGCTCTG CATCCAGACA CTAACTCCAT TGATGGGACA 
GCGGAGCAGC CTGTGCTGAA CATGAAGGAA CTGATCCCAG GCCTGCAGCC CCTTGTGCTG 
TGGATGTCCA ACTCCATTGA GCTGCTGCAC TTCATACAAC ATGAGGTCCC TCTGCTACTA 
ACATGGAGAC AGCAGGACAG ACAGGAACAG GACAAAGAAT GGCTGGATGG TCAGATCCAG 
TGCACTCGTG CTGCCAGTGA GGAGGCCATG ACGGTCCTGG AGGAGGTCAT CATGTTCACC 
TTCCAGCAGT GCGTCTATTA TCTTACCAAA ATTCTGTATG CAGTGTTGTC GGGGCTGCTG 
GACAGTAACC CTTTCTCCGA GAGCGGTCAG CTGCGCATGC CTGCGGGAGT CAGTCAGATC 
CTGGACGTCC TGAAGGAGGC TCTGCATCTG CTTAATACTT TCCAGGTGCA TCGAGAGATC 
ACGTCTCAAC TGCTGACCTA CCTGTTCTTC TTCACCAACG CCTCGCTCTT CAACACACTC 
ATGGAAAGAG GGTCTGGAGG TGGCTTCTAC CAGTGGTCCA GAGGAGTTCA GATCCGAGCC 
AACCTGGACT TACTGATGGA CTGGATTCAG AGCATTGGTC TTGGAGATTT GGCTGCTGAG 
TTCTTCCAGA AGCTCTCTTC AGCTGTGAAC CTGCTGGCAA CGCCCAAAGA GACTCTACTG 
CAGGCCTCGT GGTCCACCCT GAGAGCTGAG TTTGTTCATC TGAACCCTGC GCAGCTTCAT 
CACATGCTGA GAGAGTACAA CACATCTCGC ACATGTCCTC CATGCTGGAC TCCGTCTCCA 
GAGGACGCAG CGGCGGCGCT CCATACCTCT AATATCCTGG AAAGTTTCGA CAACCATCCT 
CCTCTAATCC TGCCCAGCAA CATTTTTCAT CTGGAGCTGG GAAAACCCAT CGCAGAGCCA 
GGGCTGTTCA CTCCTCTCCA GCGTCTGCAG GAGTTTATCC AGACCCTGTC GGACTGCCAG 
ACACAGCCTG ACCATCAGCC CGCACCGGAC TCGTCCAGTA ACCTGACTGA AGAGTCGACA 
TCTAAATCAG TCACCATCCA GAATCACGAA TCAAATCATC AGCATCACGT CTCAAGAACA 
CCTCCTCAGT TTAGGGCGTC TCACAATAAC CTGAGTTCCT GTGAGGCCGT CCTGACCCAG 
AAACTCAAGT CTCTGGAGCT TCAGAACTGC TTGTCTGGGA ACGCAGACCT GGTCTTCCAC 
AAGAGTCTGG CCCTGGACCC ATCCTGCCTG CTGACGCCCC CTAACACCCC ACAGAACCTG 
GACCAGGCCG AGCTGGAGGC CAGTCTGCAG GACGGGGGAC AGCAGAGCAT ACGCCAGCAC 
CACAGAGAGA ACGGCCTCTC AGAAAACACA GCTCAAAGAA AAGACGAGGA TGAGGATGAC 
AGAGATGAAG TCTTCACTGT TGAGCTGAAG AAAGGCCCTC ATGGTCTCGG GCTGGCGTTG 
GTGGATGGAC AGAAAACTGC TCTGCGGGTG AATGGGGTCT ATGTGAAGTC AGTGGTGCCC 
GATTCTCCAG CGGCCCAGAG CCAGAGCCTG AGAACAGGAG ACCGGATCCT CGCCGTCAAC 
GGAGTCAGTC TGGTGGGAAT GGACTACCAC AGTGGACGAG AGCTCATCCG CACATCAGGA 
GATTCACTCC GCTTACTGGT GGCCAAAACT GAGTCCATGA GCAGCGCCTG GAGCTGCATC 
ACCAGATGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch73-281f12.4 ( NM_001285388.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001272317.1 Danio rerio si:ch73-281f12.4 (si:ch73-281f12.4), mRNA.

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CloneID	ODa01836
Clone ID Related Accession (Same CDS sequence)	NM_001285388.1
Accession Version	NM_001285388.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Danio rerio(Zebrafish)
Product	si:ch73-281f12.4
Comment

ATGATTTCGG AGGAAAGGAG CAATCACGTC TCTAAACAGG CTCTGGGCTT TCCTGTCGGA 
CTGCTGATCC GCTCGCCCAA GCGAAAACTA GCGATGCTGG GCAGAAAGGC GAGCAATGGA 
TCTCTACAGT CCAGCACGTC CGACAGCACG TCTCAGTCTG TGGAGAGCAC AGGAGTCCGG 
CAGCCGGCCC GCAGCAAAAT ACGCCGCCAC AACAACCGGC TGACCACCGT CTTCAACCGA 
AGCCCCATCC TGCAGAGCGA ACGGCAGCGG CAGGATGCCC GGAGCCTGGA CCTGCAAATG 
GAGGAGGACG ACCCTGCAGA GCTGTCCAGC CAGAGGACAG TCCCGGGCAT CCTGAAGATA 
TTCGGTAGTG ACATCTGCCA GGGGACCAAC TATAAGAGCG TGCTGGCCAC CAGTAACTCC 
AGTGCCAACC AGCTGGTCAA AGAGGCTCTG GAGAGGTACT GCTTGGAAAA GGAAGACCCC 
AGCGCTTATG TGCTCTGCGA TGTGATTGGA CGAACTGGAT CCGATCACGA GTGGAAGACT 
GAGTGCTTTC GTATTGTTGG TGATTATGAG AAGCCGCTCA TGTTGCAGTC ACTCTGGAAA 
CCCAAGGAGG GATTCTCCAG ACGCTTCGAG ATCCAGAAGA GAGCAACTGT AGAGGCGCAA 
AGTGCCAAGG ATACAGACAC CATCACTGCA GGACTCAATG CCCAGGCCCG AAAGCTGCAG 
CATGCCCGTT CACGGGTCAC CTCCCTGTTT GTGGACGGGA CGCACGAGGG AGTGGACGCT 
CTGGGCATCT GGAGGAGCTT AAGTGACATG GACGTCTCCA CTATGGGAAA AGAAGCCAGC 
ATGAGTCGTC CTGCGGCCCC GCGGGATGAT CCCGAAGCAG AGGCGGATAA AGAGGTGCAG 
CGTCTCAATG CGGAGAAAGA GGAGACGGAA AGCAGCGACG ACAACCACAC GCAGTACTCC 
ATCCACCTTC CCTTTGATTT CCCATATTTC CTCCTCCTGC AAGGATACAG TCACAGACAG 
GATTTTCTGA TCTATCTGAT GAGCGGGAAC ACGTGTGTGT TCGGCAGCTC CTCTGAATCC 
TCCACCGGAG TGAACCAGGA GTCCCTGAAG GTGGACATTT TACTCTTCGC TCCTGATGTT 
TCACCTCAGC ACTGCTGCGT TCAGCGACAG GATGCCACCG CTGACCAAAC TAAGATGCAA 
ACCAAACTCA GACCACTGCA CAGTGCACAC GTTACGCACA ACGGCGTCCC GCTCCAGGAC 
GAAATCGAGT TGTGTCCAGG CGACCTGATA GGCCTCGGTC AGCACTATCT CTTCATGTTC 
AAAGACCCGA CGGCCACGGG AGCCTTTCAG ACTCCGTCCT GGATGGCTAC ATTGTGTCCC 
CCAGCCACGA CGTCTCCCTG TAAACTGTGT GGGACGTCAG TAAGGAGGAG GAGGACTCAA 
AAAATGGCCG CTCGCTGGAG GGATTCTGAT GGTAGGGTGG TTTCGCTGTC CTATCAAATG 
GAGCAGGAGG ATCGGGTGTT GGAGAAGATC GTCAGTATGG TGGATGCAGG AGGAGAAGAT 
CCCAAACTGA CCGCAGCCTT TCTGCTGTGT CTGTGCATCC AACAATCTGC CACTGACTTT 
GAGCTGCTTC ATCTACGGAG ACTGCTGCTC TCCATTGCCA ATCACATACA GCTCGACATG 
TGGGAACGAA CAAAGGAACT TGCAGCTCTG CATCCAGACA CTAACTCCAT TGATGGGACA 
GCGGAGCAGC CTGTGCTGAA CATGAAGGAA CTGATCCCAG GCCTGCAGCC CCTTGTGCTG 
TGGATGTCCA ACTCCATTGA GCTGCTGCAC TTCATACAAC ATGAGGTCCC TCTGCTCTTA 
ACATGGAGAC AGCAGGACAG ACAGGAACAG GACAAAGAAT GGCTGGATGG TCAGATCCAG 
TGCACTCGTG CTGCCAGTGA GGAGGCCATG ACGGTCCTGG AGGAGGTCAT CATGTTCACC 
TTCCAGCAGT GCGTCTACTA TCTTACCAAA ATTCTGTATG CAGTGTTGTC GGGGCTGCTG 
GACAGTAACC CTTTCTCCGA GAGCGGTCAG CTGCGCATGC CTGCGGGAGT CAGTCAGATC 
CTGGACGTCC TGAAGGAGGC TCTGCATCTG CTTAATACTT TCCAGGTGCA TCGAGAGATC 
ACGTCTCAAC TGCTGACCTA CCTGTTCTTC TTCACCAACG CCTCGCTCTT CAACACACTC 
ATGGAAAGAG GGTCTGGAGG TGGCTTCTAC CAGTGGTCCA GAGGAGTGCA GATCCGAGCC 
AACCTGGACT TACTGATGGA CTGGATTCAG AGCATTGGTC TTGGAGATTT GGCTGCTGAG 
TTCTTCCAGA AGCTCTCTTC AGCTGTGAAC CTGCTGGCAA CGCCCAAAGA GACTCTACTG 
CAGGCCTCGT GGTCCACGCT GAGAGCTGAG TTTGTTCATC TGAACCCTGC GCAGCTTCAT 
CACATGCTGA GAGAGTACAA CACATCTCGT ACATGTCCTC CATGCTGGAC TCCGTCTCCA 
GAGGACGCAG CGGCAGCCCT TCATACCTCT AATATCCTGG AAAGTTTCGA CAACCATCCT 
CCTCTAATCC TGCCCAGCAA CATTTTTCAT CTGGAGCTGG GAAAACCCAT CGCAGAGCCA 
GGGCTGTTCA CTCCTCTCCA GCGTCTGCAG GAGTTTATCC AGACCCTGTC GGACTGCCAG 
ACACAGCCTG ACCATCAGCC CGCACCGGAC TCGTCCAATA ACCTGACTGA AGAGTCGACA 
TCTAAATCAG TCACCATCCA GAATCACGAA TCAAACCATC AACATCATGT CTCGACGACG 
CCTCCTCAGT TCAGGGCGTC TCACAATAAC CTGAGCTCCT GTGAGGCCGT CCTGACCCAG 
AAACTCAAGT CCCTGGAGCT CCAGAACTGC TTGTCTGGGA ACGCAGACCT GGTCTTCCAC 
AAGAGTCTGG CCCTGGACCC ATCCTGCCTG CTGACGCCCC CTAACACCCC ACAGAACCTG 
GACCAGGCCG AGCTGGAGGC CAGTCTGCAG GACGGGGCAC AGCAGAGCAT ACGCCAGCAC 
CACAGAGTAG AGAACGGCCT CTCAGAAAAC ACAGCTCAAA GAAAAGACGA GGATGAGGAT 
GACAGAGATG AAGTCTTCAC TGTGGAGCTG AAGAAAGGCC CTCATGGTCT CGGGCTGGCG 
TTGGTGGATG GACAGAAAAC TGCTCTGCGG GTGAATGGGG TCTATGTGAA GTCAGTGGTG 
CCCGATTCTC CAGCGGCCCA GAGCCAGAGC CTGAGAACAG GAGACCGAAT CCTCGCCGTC 
AACGGAGTCA GTCTGGTGGG AATGGACTAT CACAGTGGAC GAGAGCTCAT CCGCACATCA 
GGAGATTCAC TCCGCTTACT GGTGGCCAAA ACTGAGTCCA TGAGCAGCGC CAGGAGCTGC 
ATCACCAGAT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_001272317.1

Translation

MISEERSNHVSKQALGFPVGLLIRSPKRKLAMLGRKASNGSLQSSTSDSTSQSVESTGVRQPARSKIRRHNNRLTTVFNRSPILQSERQRQDARSLDLQMEEDDPAELSSQRTVPGILKIFGSDICQGTNYKSVLATSNSSANQLVKEALERYCLEKEDPSAYVLCDVIGRTGSDHEWKTECFRIVGDYEKPLMLQSLWKPKEGFSRRFEIQKRATVEAQSAKDTDTITAGLNAQARKLQHARSRVTSLFVDGTHEGVDALGIWRSLSDMDVSTMGKEASMSRPAAPRDDPEAEADKEVQRLNAEKEETESSDDNHTQYSIHLPFDFPYFLLLQGYSHRQDFLIYLMSGNTCVFGSSSESSTGVNQESLKVDILLFAPDVSPQHCCVQRQDATADQTKMQTKLRPLHSAHVTHNGVPLQDEIELCPGDLIGLGQHYLFMFKDPTATGAFQTPSWMATLCPPATTSPCKLCGTSVRRRRTQKMAARWRDSDGRVVSLSYQMEQEDRVLEKIVSMVDAGGEDPKLTAAFLLCLCIQQSATDFELLHLRRLLLSIANHIQLDMWERTKELAALHPDTNSIDGTAEQPVLNMKELIPGLQPLVLWMSNSIELLHFIQHEVPLLLTWRQQDRQEQDKEWLDGQIQCTRAASEEAMTVLEEVIMFTFQQCVYYLTKILYAVLSGLLDSNPFSESGQLRMPAGVSQILDVLKEALHLLNTFQVHREITSQLLTYLFFFTNASLFNTLMERGSGGGFYQWSRGVQIRANLDLLMDWIQSIGLGDLAAEFFQKLSSAVNLLATPKETLLQASWSTLRAEFVHLNPAQLHHMLREYNTSRTCPPCWTPSPEDAAAALHTSNILESFDNHPPLILPSNIFHLELGKPIAEPGLFTPLQRLQEFIQTLSDCQTQPDHQPAPDSSNNLTEESTSKSVTIQNHESNHQHHVSTTPPQFRASHNNLSSCEAVLTQKLKSLELQNCLSGNADLVFHKSLALDPSCLLTPPNTPQNLDQAELEASLQDGAQQSIRQHHRVENGLSENTAQRKDEDEDDRDEVFTVELKKGPHGLGLALVDGQKTALRVNGVYVKSVVPDSPAAQSQSLRTGDRILAVNGVSLVGMDYHSGRELIRTSGDSLRLLVAKTESMSSARSCITRC

Target ORF information:

RefSeq Version	NM_001285388.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch73-281f12.4 (si:ch73-281f12.4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001285388.1

ORF Insert Sequence:

ATGATTTCGG AGGAAAGGAG CAATCACGTC TCTAAACAGG CTCTGGGCTT TCCTGTCGGA 
CTGCTGATCC GCTCGCCCAA GCGAAAACTA GCGATGCTGG GCAGAAAGGC GAGCAATGGA 
TCTCTACAGT CCAGCACGTC CGACAGCACG TCTCAGTCTG TGGAGAGCAC AGGAGTCCGG 
CAGCCGGCCC GCAGCAAAAT ACGCCGCCAC AACAACCGGC TGACCACCGT CTTCAACCGA 
AGCCCCATCC TGCAGAGCGA ACGGCAGCGG CAGGATGCCC GGAGCCTGGA CCTGCAAATG 
GAGGAGGACG ACCCTGCAGA GCTGTCCAGC CAGAGGACAG TCCCGGGCAT CCTGAAGATA 
TTCGGTAGTG ACATCTGCCA GGGGACCAAC TATAAGAGCG TGCTGGCCAC CAGTAACTCC 
AGTGCCAACC AGCTGGTCAA AGAGGCTCTG GAGAGGTACT GCTTGGAAAA GGAAGACCCC 
AGCGCTTATG TGCTCTGCGA TGTGATTGGA CGAACTGGAT CCGATCACGA GTGGAAGACT 
GAGTGCTTTC GTATTGTTGG TGATTATGAG AAGCCGCTCA TGTTGCAGTC ACTCTGGAAA 
CCCAAGGAGG GATTCTCCAG ACGCTTCGAG ATCCAGAAGA GAGCAACTGT AGAGGCGCAA 
AGTGCCAAGG ATACAGACAC CATCACTGCA GGACTCAATG CCCAGGCCCG AAAGCTGCAG 
CATGCCCGTT CACGGGTCAC CTCCCTGTTT GTGGACGGGA CGCACGAGGG AGTGGACGCT 
CTGGGCATCT GGAGGAGCTT AAGTGACATG GACGTCTCCA CTATGGGAAA AGAAGCCAGC 
ATGAGTCGTC CTGCGGCCCC GCGGGATGAT CCCGAAGCAG AGGCGGATAA AGAGGTGCAG 
CGTCTCAATG CGGAGAAAGA GGAGACGGAA AGCAGCGACG ACAACCACAC GCAGTACTCC 
ATCCACCTTC CCTTTGATTT CCCATATTTC CTCCTCCTGC AAGGATACAG TCACAGACAG 
GATTTTCTGA TCTATCTGAT GAGCGGGAAC ACGTGTGTGT TCGGCAGCTC CTCTGAATCC 
TCCACCGGAG TGAACCAGGA GTCCCTGAAG GTGGACATTT TACTCTTCGC TCCTGATGTT 
TCACCTCAGC ACTGCTGCGT TCAGCGACAG GATGCCACCG CTGACCAAAC TAAGATGCAA 
ACCAAACTCA GACCACTGCA CAGTGCACAC GTTACGCACA ACGGCGTCCC GCTCCAGGAC 
GAAATCGAGT TGTGTCCAGG CGACCTGATA GGCCTCGGTC AGCACTATCT CTTCATGTTC 
AAAGACCCGA CGGCCACGGG AGCCTTTCAG ACTCCGTCCT GGATGGCTAC ATTGTGTCCC 
CCAGCCACGA CGTCTCCCTG TAAACTGTGT GGGACGTCAG TAAGGAGGAG GAGGACTCAA 
AAAATGGCCG CTCGCTGGAG GGATTCTGAT GGTAGGGTGG TTTCGCTGTC CTATCAAATG 
GAGCAGGAGG ATCGGGTGTT GGAGAAGATC GTCAGTATGG TGGATGCAGG AGGAGAAGAT 
CCCAAACTGA CCGCAGCCTT TCTGCTGTGT CTGTGCATCC AACAATCTGC CACTGACTTT 
GAGCTGCTTC ATCTACGGAG ACTGCTGCTC TCCATTGCCA ATCACATACA GCTCGACATG 
TGGGAACGAA CAAAGGAACT TGCAGCTCTG CATCCAGACA CTAACTCCAT TGATGGGACA 
GCGGAGCAGC CTGTGCTGAA CATGAAGGAA CTGATCCCAG GCCTGCAGCC CCTTGTGCTG 
TGGATGTCCA ACTCCATTGA GCTGCTGCAC TTCATACAAC ATGAGGTCCC TCTGCTCTTA 
ACATGGAGAC AGCAGGACAG ACAGGAACAG GACAAAGAAT GGCTGGATGG TCAGATCCAG 
TGCACTCGTG CTGCCAGTGA GGAGGCCATG ACGGTCCTGG AGGAGGTCAT CATGTTCACC 
TTCCAGCAGT GCGTCTACTA TCTTACCAAA ATTCTGTATG CAGTGTTGTC GGGGCTGCTG 
GACAGTAACC CTTTCTCCGA GAGCGGTCAG CTGCGCATGC CTGCGGGAGT CAGTCAGATC 
CTGGACGTCC TGAAGGAGGC TCTGCATCTG CTTAATACTT TCCAGGTGCA TCGAGAGATC 
ACGTCTCAAC TGCTGACCTA CCTGTTCTTC TTCACCAACG CCTCGCTCTT CAACACACTC 
ATGGAAAGAG GGTCTGGAGG TGGCTTCTAC CAGTGGTCCA GAGGAGTGCA GATCCGAGCC 
AACCTGGACT TACTGATGGA CTGGATTCAG AGCATTGGTC TTGGAGATTT GGCTGCTGAG 
TTCTTCCAGA AGCTCTCTTC AGCTGTGAAC CTGCTGGCAA CGCCCAAAGA GACTCTACTG 
CAGGCCTCGT GGTCCACGCT GAGAGCTGAG TTTGTTCATC TGAACCCTGC GCAGCTTCAT 
CACATGCTGA GAGAGTACAA CACATCTCGT ACATGTCCTC CATGCTGGAC TCCGTCTCCA 
GAGGACGCAG CGGCAGCCCT TCATACCTCT AATATCCTGG AAAGTTTCGA CAACCATCCT 
CCTCTAATCC TGCCCAGCAA CATTTTTCAT CTGGAGCTGG GAAAACCCAT CGCAGAGCCA 
GGGCTGTTCA CTCCTCTCCA GCGTCTGCAG GAGTTTATCC AGACCCTGTC GGACTGCCAG 
ACACAGCCTG ACCATCAGCC CGCACCGGAC TCGTCCAATA ACCTGACTGA AGAGTCGACA 
TCTAAATCAG TCACCATCCA GAATCACGAA TCAAACCATC AACATCATGT CTCGACGACG 
CCTCCTCAGT TCAGGGCGTC TCACAATAAC CTGAGCTCCT GTGAGGCCGT CCTGACCCAG 
AAACTCAAGT CCCTGGAGCT CCAGAACTGC TTGTCTGGGA ACGCAGACCT GGTCTTCCAC 
AAGAGTCTGG CCCTGGACCC ATCCTGCCTG CTGACGCCCC CTAACACCCC ACAGAACCTG 
GACCAGGCCG AGCTGGAGGC CAGTCTGCAG GACGGGGCAC AGCAGAGCAT ACGCCAGCAC 
CACAGAGTAG AGAACGGCCT CTCAGAAAAC ACAGCTCAAA GAAAAGACGA GGATGAGGAT 
GACAGAGATG AAGTCTTCAC TGTGGAGCTG AAGAAAGGCC CTCATGGTCT CGGGCTGGCG 
TTGGTGGATG GACAGAAAAC TGCTCTGCGG GTGAATGGGG TCTATGTGAA GTCAGTGGTG 
CCCGATTCTC CAGCGGCCCA GAGCCAGAGC CTGAGAACAG GAGACCGAAT CCTCGCCGTC 
AACGGAGTCA GTCTGGTGGG AATGGACTAT CACAGTGGAC GAGAGCTCAT CCGCACATCA 
GGAGATTCAC TCCGCTTACT GGTGGCCAAA ACTGAGTCCA TGAGCAGCGC CAGGAGCTGC 
ATCACCAGAT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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si:ch73-281f12.4 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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