prkcha cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	prkcha
Entrez Gene ID	100144561
Full Name	protein kinase C, eta, a
Synonyms	zgc:172124
General protein information	Preferred Names protein kinase C, eta, a Names protein kinase C eta type
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001123056.1	NP_001116528.1	protein kinase C eta type

Pathways from BioSystems

Homologies

	PRKCH	XP_002805131.1
Rattus norvegicus (Norway rat)	Prkch	NP_112347.1
Xenopus tropicalis (tropical clawed frog)	prkch	XP_002939521.2
Canis lupus familiaris (dog)	PRKCH	XP_547844.2
Gallus gallus (chicken)	PRKCH	XP_421417.3
Danio rerio (zebrafish)	prkcha	NP_001116528.1
Homo sapiens (human)	PRKCH	NP_006246.2
Bos taurus (cattle)	PRKCH	NP_001070331.1
Mus musculus (house mouse)	Prkch	NP_032882.2
Pan troglodytes (chimpanzee)	PRKCH	XP_001168420.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Evolutionary comparison reveals that diverging CTCF sites are signatures of ancestral topological associating domains borders.
G?mez-Mar?n C, Tena JJ, Acemel RD, L?pez-Mayorga M, Naranjo S, de la Calle-Mustienes E, Maeso I, Beccari L, Aneas I, Vielmas E, Bovolenta P, Nobrega MA, Carvajal J, G?mez-Skarmeta JL
Proceedings of the National Academy of Sciences of the United States of America112(24)7542-7(2015 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following prkcha gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prkcha cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa33056	NM_001123056.1 Latest version!	Danio rerio protein kinase C, eta, a (prkcha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prkcha ( NM_001123056.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001116528.1 Danio rerio protein kinase C, eta, a (prkcha), mRNA.

Price & Availability↑

CloneID	ODa33056
Clone ID Related Accession (Same CDS sequence)	NM_001123056.1
Accession Version	NM_001123056.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	protein kinase C eta type
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC160622.1. On Jul 31, 2008 this sequence version replaced XM_001922909.1. ##Evidence-Data-START## Transcript exon combination :: BC160622.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAAGTTTA ACGGATATCT GAAGTTGCGT ATCGGTGAAG CGGTGGACCT GAAGCCCACG 
ACCACCTCCA TGCGCCACGC CGTGATGTTC AACAAGGCGA CCCAAACACT AGACCCCTAC 
ATAGTCGTGA AAGTGGACGA GTATAAGATC GGACAGACGC ACACCAAGCT GAAAACCAAC 
ATGCCCACTT ATAACGAGGA GTTCTCCGTT AACGTAAACA ACGGTAAGCA AGTGGAGCTC 
GCCGTTTTTC ATGACGCACC GATCGGTTAC GATGACTTTG TGGCCAACTG TACTATCCAG 
TTTGACGATC TGATGAAGAC CAACAGCAGA GAGGAGTCTT TTGAAGGATG GGTTGATCTT 
GAACCTGAGG GTAAGGTTTA CATCCTGGTT ACTCTCACAG GCTCATTTAT TGATGAGGAT 
GCTGGAGGAA ACGCGCGCCC CTACAAAGAG TTTAATCGAA AACGGCAACA GGCAGTCAGA 
AGAAAAATTC ACCAAGTCAA CGGGCACAAA TTCATGTCAA CTTTCTTAAA GCAACCAACA 
TTCTGTTTCC ACTGCAAAGA GTTCATATGG GGTGTTTTTG GAAAACAGGG CTACCAATGT 
CAAGTTTGCA CCTGTGTTGT GCACAAGCGA TGCCATCAGC TTGTGGTCAC GGTTTGTCCA 
CGCATGAAAA AACCTGCCAA GGAGCAGAGT ACAACTCAGG GTTTCAGTAT AAACGTTCCC 
CACAAGTTCA GCATCCACAA TTATAAAGTT CCCACCTTCT GTGACCACTG CGGCTCTTTG 
CTGTGGGGCA TCGTACGTCA AGGATTGCAC TGCAAAATTT GTAAAATGAA TGTCCATATA 
CGCTGTAAAG GAAATGTCGC ACCAAACTGT GGAATCAACA ATGTTGAACT GGCAAATAAA 
CTGGCAGAAA TGGGCCTTCA GCCTGGAGGA CTGACCAAAC GCAACTCAGT GGGTCTTGCA 
GGTTTGGACG CTCAGTCCCG GACACTGAGT GTTAGAGGAG AACGAGGGCA GTCTAAAAAA 
GAGCAGTGTC TGAAACTGGG AATCTCCAAC TTCACATTGT TGCAGGTGTT GGGAAAAGGC 
AGCTTTGGCA AGGTGATGTT GGTCCGGCTG AACGGGTCAG ATCAGGTGTT TGCAGTAAAA 
ATCCTGAAGA AAGACATCAT ACTGCAGGAT GATGATGTGG AGTGCACTAT GACAGAAAAA 
CGAGTGTTAT CTCTCGCTTC CACTCATCCT TACCTCACCC AGCTCTACTG CTGCTTCCAG 
ACACCGGAGC GTTTGTTTTT CGTAATGGAG TTTGTGAATG GAGGAGATCT GATGTTTCAT 
ATTCAGAAAT CCAGAAAGTT TGAGGAGAAC CGAGCCAGAT TTTACACAGC GGAGATCACA 
TCTGCGCTCA TATTCCTCCA CAGCAAGGGG ATTGTATACA GGGATCTAAA GCTGGATAAT 
GTTTTGCTGG ATAAGGATGG TCATTGTAAA CTGGCTGATT TCGGCATGTG TAAGGAAGGG 
ATGTTTGACG GTATTGTGAC TGGAACCTTC TGTGGAACCC CAGATTACAT TGCACCAGAG 
ATTCTCCAGG AATTACTGTA CGGGCCGTCG GTTGATTGGT GGGCTCTCGG TGTGCTGCTC 
TATGAGATGC TCTCAGGCCA CGCCCCCTTC GAGGCAGAGA ACGAGGATGA CCTTTTTGAG 
TCCATCCTGA CGGAAGAGAT CATCTACGCA TCCTGGCTCA GCACAAACGC TGTCGACATA 
CTGAAAGGGT TTCTGACAAA GAATCCATCC CGACGCCTGG GTTGTGTAGC TACAGAGGGT 
GGAGAGACTG CTGTAACCGG TCATCCATTC TTTACCGACA TCGACTGGGA CAAACTTGAC 
CAACGAGCGC TTGAACCACC TTTCAAGCCC AGAATTAAAA CTGCAGAAGA TGTGAATAAC 
TTTGATCCAG ACTTCACCCA AGAGGATCCT ACACTTACAC CCATCGATGA GTCCATCCTC 
CCATCCATCA GTCAGGATGA CTTCAGGGGC TTCTCCTTTA CCTCTTCTGA ACTCTTAAAA 
ATGGTTTCCG ATTTCCAAAG AGCTGAAGAA ACAAGTGATG TCAAGAAAGA ATGGACCTCA 
GGAGAATTCT GTGATTTCGT TGGACCAAAT GGATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116528.1
CDS	159..2297
Translation	MKFNGYLKLRIGEAVDLKPTTTSMRHAVMFNKATQTLDPYIVVKVDEYKIGQTHTKLKTNMPTYNEEFSVNVNNGKQVELAVFHDAPIGYDDFVANCTIQFDDLMKTNSREESFEGWVDLEPEGKVYILVTLTGSFIDEDAGGNARPYKEFNRKRQQAVRRKIHQVNGHKFMSTFLKQPTFCFHCKEFIWGVFGKQGYQCQVCTCVVHKRCHQLVVTVCPRMKKPAKEQSTTQGFSINVPHKFSIHNYKVPTFCDHCGSLLWGIVRQGLHCKICKMNVHIRCKGNVAPNCGINNVELANKLAEMGLQPGGLTKRNSVGLAGLDAQSRTLSVRGERGQSKKEQCLKLGISNFTLLQVLGKGSFGKVMLVRLNGSDQVFAVKILKKDIILQDDDVECTMTEKRVLSLASTHPYLTQLYCCFQTPERLFFVMEFVNGGDLMFHIQKSRKFEENRARFYTAEITSALIFLHSKGIVYRDLKLDNVLLDKDGHCKLADFGMCKEGMFDGIVTGTFCGTPDYIAPEILQELLYGPSVDWWALGVLLYEMLSGHAPFEAENEDDLFESILTEEIIYASWLSTNAVDILKGFLTKNPSRRLGCVATEGGETAVTGHPFFTDIDWDKLDQRALEPPFKPRIKTAEDVNNFDPDFTQEDPTLTPIDESILPSISQDDFRGFSFTSSELLKMVSDFQRAEETSDVKKEWTSGEFCDFVGPNGL

Target ORF information:

RefSeq Version	NM_001123056.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein kinase C, eta, a (prkcha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001123056.1

ORF Insert Sequence:

ATGAAGTTTA ACGGATATCT GAAGTTGCGT ATCGGTGAAG CGGTGGACCT GAAGCCCACG 
ACCACCTCCA TGCGCCACGC CGTGATGTTC AACAAGGCGA CCCAAACACT AGACCCCTAC 
ATAGTCGTGA AAGTGGACGA GTATAAGATC GGACAGACGC ACACCAAGCT GAAAACCAAC 
ATGCCCACTT ATAACGAGGA GTTCTCCGTT AACGTAAACA ACGGTAAGCA AGTGGAGCTC 
GCCGTTTTTC ATGACGCACC GATCGGTTAC GATGACTTTG TGGCCAACTG TACTATCCAG 
TTTGACGATC TGATGAAGAC CAACAGCAGA GAGGAGTCTT TTGAAGGATG GGTTGATCTT 
GAACCTGAGG GTAAGGTTTA CATCCTGGTT ACTCTCACAG GCTCATTTAT TGATGAGGAT 
GCTGGAGGAA ACGCGCGCCC CTACAAAGAG TTTAATCGAA AACGGCAACA GGCAGTCAGA 
AGAAAAATTC ACCAAGTCAA CGGGCACAAA TTCATGTCAA CTTTCTTAAA GCAACCAACA 
TTCTGTTTCC ACTGCAAAGA GTTCATATGG GGTGTTTTTG GAAAACAGGG CTACCAATGT 
CAAGTTTGCA CCTGTGTTGT GCACAAGCGA TGCCATCAGC TTGTGGTCAC GGTTTGTCCA 
CGCATGAAAA AACCTGCCAA GGAGCAGAGT ACAACTCAGG GTTTCAGTAT AAACGTTCCC 
CACAAGTTCA GCATCCACAA TTATAAAGTT CCCACCTTCT GTGACCACTG CGGCTCTTTG 
CTGTGGGGCA TCGTACGTCA AGGATTGCAC TGCAAAATTT GTAAAATGAA TGTCCATATA 
CGCTGTAAAG GAAATGTCGC ACCAAACTGT GGAATCAACA ATGTTGAACT GGCAAATAAA 
CTGGCAGAAA TGGGCCTTCA GCCTGGAGGA CTGACCAAAC GCAACTCAGT GGGTCTTGCA 
GGTTTGGACG CTCAGTCCCG GACACTGAGT GTTAGAGGAG AACGAGGGCA GTCTAAAAAA 
GAGCAGTGTC TGAAACTGGG AATCTCCAAC TTCACATTGT TGCAGGTGTT GGGAAAAGGC 
AGCTTTGGCA AGGTGATGTT GGTCCGGCTG AACGGGTCAG ATCAGGTGTT TGCAGTAAAA 
ATCCTGAAGA AAGACATCAT ACTGCAGGAT GATGATGTGG AGTGCACTAT GACAGAAAAA 
CGAGTGTTAT CTCTCGCTTC CACTCATCCT TACCTCACCC AGCTCTACTG CTGCTTCCAG 
ACACCGGAGC GTTTGTTTTT CGTAATGGAG TTTGTGAATG GAGGAGATCT GATGTTTCAT 
ATTCAGAAAT CCAGAAAGTT TGAGGAGAAC CGAGCCAGAT TTTACACAGC GGAGATCACA 
TCTGCGCTCA TATTCCTCCA CAGCAAGGGG ATTGTATACA GGGATCTAAA GCTGGATAAT 
GTTTTGCTGG ATAAGGATGG TCATTGTAAA CTGGCTGATT TCGGCATGTG TAAGGAAGGG 
ATGTTTGACG GTATTGTGAC TGGAACCTTC TGTGGAACCC CAGATTACAT TGCACCAGAG 
ATTCTCCAGG AATTACTGTA CGGGCCGTCG GTTGATTGGT GGGCTCTCGG TGTGCTGCTC 
TATGAGATGC TCTCAGGCCA CGCCCCCTTC GAGGCAGAGA ACGAGGATGA CCTTTTTGAG 
TCCATCCTGA CGGAAGAGAT CATCTACGCA TCCTGGCTCA GCACAAACGC TGTCGACATA 
CTGAAAGGGT TTCTGACAAA GAATCCATCC CGACGCCTGG GTTGTGTAGC TACAGAGGGT 
GGAGAGACTG CTGTAACCGG TCATCCATTC TTTACCGACA TCGACTGGGA CAAACTTGAC 
CAACGAGCGC TTGAACCACC TTTCAAGCCC AGAATTAAAA CTGCAGAAGA TGTGAATAAC 
TTTGATCCAG ACTTCACCCA AGAGGATCCT ACACTTACAC CCATCGATGA GTCCATCCTC 
CCATCCATCA GTCAGGATGA CTTCAGGGGC TTCTCCTTTA CCTCTTCTGA ACTCTTAAAA 
ATGGTTTCCG ATTTCCAAAG AGCTGAAGAA ACAAGTGATG TCAAGAAAGA ATGGACCTCA 
GGAGAATTCT GTGATTTCGT TGGACCAAAT GGATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Evolutionary comparison reveals that diverging CTCF sites are signatures of ancestral topological associating domains borders.
Proceedings of the National Academy of Sciences of the United States of America112(24)7542-7(2015 Jun)
G?mez-Mar?n C,Tena JJ,Acemel RD,L?pez-Mayorga M,Naranjo S,de la Calle-Mustienes E,Maeso I,Beccari L,Aneas I,Vielmas E,Bovolenta P,Nobrega MA,Carvajal J,G?mez-Skarmeta JL

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prkcha cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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